AU2001236839A1 - Multi-gene expression constructs containing modified inteins - Google Patents

Multi-gene expression constructs containing modified inteins

Info

Publication number
AU2001236839A1
AU2001236839A1 AU2001236839A AU3683901A AU2001236839A1 AU 2001236839 A1 AU2001236839 A1 AU 2001236839A1 AU 2001236839 A AU2001236839 A AU 2001236839A AU 3683901 A AU3683901 A AU 3683901A AU 2001236839 A1 AU2001236839 A1 AU 2001236839A1
Authority
AU
Australia
Prior art keywords
protein
plants
fused
constructs
splicing unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001236839A
Inventor
Kristi D. Snell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yield10 Bioscience Inc
Original Assignee
Metabolix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolix Inc filed Critical Metabolix Inc
Publication of AU2001236839A1 publication Critical patent/AU2001236839A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Abstract

Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5' promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5'-terminus of the coding regions for the second protein to form a construct for expression of more than two proteins. A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and/or value added products, illustrated by the production of polyhydroxyalkanoates in plants.
AU2001236839A 2000-02-11 2001-02-09 Multi-gene expression constructs containing modified inteins Abandoned AU2001236839A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18173900P 2000-02-11 2000-02-11
US60181739 2000-02-11
PCT/US2001/004254 WO2001059091A2 (en) 2000-02-11 2001-02-09 Multi-gene expression constructs containing modified inteins

Publications (1)

Publication Number Publication Date
AU2001236839A1 true AU2001236839A1 (en) 2001-08-20

Family

ID=22665581

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001236839A Abandoned AU2001236839A1 (en) 2000-02-11 2001-02-09 Multi-gene expression constructs containing modified inteins

Country Status (7)

Country Link
US (3) US7026526B2 (en)
EP (3) EP1255846B1 (en)
AT (1) ATE454456T1 (en)
AU (1) AU2001236839A1 (en)
DE (1) DE60140996D1 (en)
ES (1) ES2336641T3 (en)
WO (1) WO2001059091A2 (en)

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US10407742B2 (en) 2009-11-06 2019-09-10 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US8420387B2 (en) 2009-11-06 2013-04-16 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
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JP6262217B2 (en) 2012-06-07 2018-01-17 ダウ アグロサイエンシィズ エルエルシー Constructs and methods for expressing transgenes using Brassica bi-directional constitutive promoters
WO2014055778A2 (en) 2012-10-03 2014-04-10 Agrivida, Inc. Multiprotein expression cassettes
RU2601126C2 (en) * 2014-09-17 2016-10-27 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России Method of producing biologically active peptides
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Also Published As

Publication number Publication date
EP2159287A2 (en) 2010-03-03
EP2159288A3 (en) 2014-02-12
WO2001059091A2 (en) 2001-08-16
US7741530B2 (en) 2010-06-22
EP2159287A3 (en) 2014-02-12
US20100251418A1 (en) 2010-09-30
WO2001059091A3 (en) 2001-12-20
US20050283850A1 (en) 2005-12-22
US20020129400A1 (en) 2002-09-12
US7026526B2 (en) 2006-04-11
US8293971B2 (en) 2012-10-23
ES2336641T3 (en) 2010-04-15
EP1255846A2 (en) 2002-11-13
DE60140996D1 (en) 2010-02-25
EP1255846B1 (en) 2010-01-06
ATE454456T1 (en) 2010-01-15
EP2159288A2 (en) 2010-03-03

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