ATE491811T1 - METHOD FOR DETECTING MUTANT ALLELES USING A COMBINATION OF REAL-TIME PCR AND REMS PCR - Google Patents

METHOD FOR DETECTING MUTANT ALLELES USING A COMBINATION OF REAL-TIME PCR AND REMS PCR

Info

Publication number
ATE491811T1
ATE491811T1 AT07820522T AT07820522T ATE491811T1 AT E491811 T1 ATE491811 T1 AT E491811T1 AT 07820522 T AT07820522 T AT 07820522T AT 07820522 T AT07820522 T AT 07820522T AT E491811 T1 ATE491811 T1 AT E491811T1
Authority
AT
Austria
Prior art keywords
pcr
real
combination
rems
mutant alleles
Prior art date
Application number
AT07820522T
Other languages
German (de)
Inventor
Daniel Adlerstein
Erlet Shehi
Giulia Amicarelli
Original Assignee
Diasorin S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diasorin S P A filed Critical Diasorin S P A
Application granted granted Critical
Publication of ATE491811T1 publication Critical patent/ATE491811T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The instant invention refers to a method for simoultaneous selective amplification and detection of mutant DNA sequences, to oligonucleotides to be used for said method and to kit for the same. The invention refers aldo to a method for identifying a specific mutated DNA sequence, to oligonucleotides to be used for said method and to kit for the same.
AT07820522T 2006-09-26 2007-09-24 METHOD FOR DETECTING MUTANT ALLELES USING A COMBINATION OF REAL-TIME PCR AND REMS PCR ATE491811T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06020128A EP1905842A1 (en) 2006-09-26 2006-09-26 Method for detection of mutant alleles combining Real Time PCR and REMS-PCR
PCT/EP2007/060116 WO2008037694A1 (en) 2006-09-26 2007-09-24 Method for detection of mutant alleles combining real time pcr and rems-pcr

Publications (1)

Publication Number Publication Date
ATE491811T1 true ATE491811T1 (en) 2011-01-15

Family

ID=37687373

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07820522T ATE491811T1 (en) 2006-09-26 2007-09-24 METHOD FOR DETECTING MUTANT ALLELES USING A COMBINATION OF REAL-TIME PCR AND REMS PCR

Country Status (7)

Country Link
US (1) US8329405B2 (en)
EP (2) EP1905842A1 (en)
AT (1) ATE491811T1 (en)
CA (1) CA2666442A1 (en)
DE (1) DE602007011301D1 (en)
ES (1) ES2354745T3 (en)
WO (1) WO2008037694A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
US20100285478A1 (en) 2009-03-27 2010-11-11 Life Technologies Corporation Methods, Compositions, and Kits for Detecting Allelic Variants
WO2010077324A2 (en) * 2008-12-17 2010-07-08 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
CN102115782B (en) * 2009-12-30 2014-06-18 北京雅康博生物科技有限公司 Kit for quantitative detection of K-ras mutation
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
CN103571947B (en) * 2013-10-09 2015-03-25 武汉百泰基因工程有限公司 Kit for detecting hotspot mutation of KRAS gene
US10433030B2 (en) 2014-10-09 2019-10-01 Thuuz, Inc. Generating a customized highlight sequence depicting multiple events
CN106048015A (en) * 2016-06-07 2016-10-26 中国疾病预防控制中心传染病预防控制所 ET-PCR (endonuclease restriction-mediated real-time polymerase chain reaction) nucleic acid testing technology
CN107937524A (en) * 2017-12-05 2018-04-20 中源协和基因科技有限公司 Mankind's KRAS gene mutation detection kit and detection method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US20060188902A1 (en) * 2005-01-03 2006-08-24 The Gov. of the USA as represented by the Secretary of the Dept. of Health and Human Primer for nucleic acid detection
EP1969140A2 (en) 2005-11-23 2008-09-17 DiaSorin S.p.A. Reagents and method for simultaneous nucleic acid amplification and detection

Also Published As

Publication number Publication date
EP2066804B1 (en) 2010-12-15
US8329405B2 (en) 2012-12-11
CA2666442A1 (en) 2008-04-03
EP2066804A1 (en) 2009-06-10
ES2354745T3 (en) 2011-03-17
DE602007011301D1 (en) 2011-01-27
US20100190157A1 (en) 2010-07-29
WO2008037694A1 (en) 2008-04-03
EP1905842A1 (en) 2008-04-02

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