ATE147860T1 - METHOD FOR IMMOBILIZING NUCLEIC ACID TO A SOLID SURFACE FOR USE IN NUCLEIC ACID HYBRIDIZATION TESTS - Google Patents

METHOD FOR IMMOBILIZING NUCLEIC ACID TO A SOLID SURFACE FOR USE IN NUCLEIC ACID HYBRIDIZATION TESTS

Info

Publication number
ATE147860T1
ATE147860T1 AT90313007T AT90313007T ATE147860T1 AT E147860 T1 ATE147860 T1 AT E147860T1 AT 90313007 T AT90313007 T AT 90313007T AT 90313007 T AT90313007 T AT 90313007T AT E147860 T1 ATE147860 T1 AT E147860T1
Authority
AT
Austria
Prior art keywords
nucleic acid
solid surface
immobilizing
acid strand
modified
Prior art date
Application number
AT90313007T
Other languages
German (de)
Inventor
Chander Bahl
Rhonda Lang
Leo Mendoza
Original Assignee
Ortho Diagnostic Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Diagnostic Systems Inc filed Critical Ortho Diagnostic Systems Inc
Application granted granted Critical
Publication of ATE147860T1 publication Critical patent/ATE147860T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.
AT90313007T 1989-11-30 1990-11-29 METHOD FOR IMMOBILIZING NUCLEIC ACID TO A SOLID SURFACE FOR USE IN NUCLEIC ACID HYBRIDIZATION TESTS ATE147860T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/444,031 US5215882A (en) 1989-11-30 1989-11-30 Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays

Publications (1)

Publication Number Publication Date
ATE147860T1 true ATE147860T1 (en) 1997-02-15

Family

ID=23763208

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90313007T ATE147860T1 (en) 1989-11-30 1990-11-29 METHOD FOR IMMOBILIZING NUCLEIC ACID TO A SOLID SURFACE FOR USE IN NUCLEIC ACID HYBRIDIZATION TESTS

Country Status (7)

Country Link
US (1) US5215882A (en)
EP (1) EP0435470B1 (en)
AT (1) ATE147860T1 (en)
CA (1) CA2031026A1 (en)
DE (1) DE69029717T2 (en)
GR (1) GR1001043B (en)
HK (1) HK95097A (en)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6309822B1 (en) * 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6406844B1 (en) 1989-06-07 2002-06-18 Affymetrix, Inc. Very large scale immobilized polymer synthesis
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US6506558B1 (en) 1990-03-07 2003-01-14 Affymetrix Inc. Very large scale immobilized polymer synthesis
IE914007A1 (en) * 1990-11-19 1992-05-20 Univ Illinois A protein being genetically engineered to provide¹orientability and a substrate having an oriented layer of¹proteins
EP1231282A3 (en) 1990-12-06 2005-05-18 Affymetrix, Inc. Methods and compositions for identification of polymers
DE69233501T2 (en) * 1991-11-22 2006-02-23 Affymetrix, Inc. (n.d.Ges.d.Staates Delaware), Santa Clara Combinatorial strategies for polymer synthesis
US6943034B1 (en) 1991-11-22 2005-09-13 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
US6468740B1 (en) 1992-11-05 2002-10-22 Affymetrix, Inc. Cyclic and substituted immobilized molecular synthesis
EP0647719A4 (en) * 1992-03-19 1997-08-20 Nippon Gene Kk Solid-phase anchor carrying nucleic acid and method of adjusting the same.
FR2716263B1 (en) * 1994-02-11 1997-01-17 Pasteur Institut Method for aligning macromolecules by passing a meniscus and applications in a method for highlighting, separating and / or assaying a macromolecule in a sample.
US7378236B1 (en) 1994-06-17 2008-05-27 The Board Of Trustees Of The Leland Stanford Junior University Method for analyzing gene expression patterns
US7323298B1 (en) 1994-06-17 2008-01-29 The Board Of Trustees Of The Leland Stanford Junior University Microarray for determining the relative abundances of polynuceotide sequences
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US20030104361A1 (en) * 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5922534A (en) * 1995-03-28 1999-07-13 Hewlett-Packard Company Dry biochemical assay plate and method for making the same
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc Nucleic acid analysis techniques
US6881571B1 (en) * 1998-03-11 2005-04-19 Exonhit Therapeutics S.A. Qualitative differential screening
US6103535A (en) * 1996-05-31 2000-08-15 University Of Maryland Optical fiber evanescent field excited fluorosensor and method of manufacture
ATE335845T1 (en) * 1997-05-16 2006-09-15 Exact Sciences Corp ELECTROPHORETIC ANALYSIS OF MOLECULES USING IMMOBILIZED PROBE
JP2002505846A (en) 1997-11-06 2002-02-26 モザイク テクノロジーズ Multiple sequential polynucleotide displacement reactions for signal amplification and processing
US6979728B2 (en) * 1998-05-04 2005-12-27 Baylor College Of Medicine Articles of manufacture and methods for array based analysis of biological molecules
US6048695A (en) * 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
WO1999066078A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
JP2002518026A (en) * 1998-06-19 2002-06-25 エムティー テクノロジー， インコーポレイテッド Detection of non-viral organisms using SRPRNA
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
DK1157264T3 (en) * 1999-02-26 2004-08-16 Exact Sciences Corp Biochemical purification devices with immobilized capture probes and applications thereof
US6194563B1 (en) * 1999-03-26 2001-02-27 Vysis, Inc. Solid phase nucleic acid labeling by transamination
US6248537B1 (en) 1999-05-28 2001-06-19 Institut Pasteur Use of the combing process for the identification of DNA origins of replication
FR2798673B1 (en) * 1999-09-16 2004-05-28 Exonhit Therapeutics Sa METHODS AND COMPOSITIONS FOR DETECTION OF PATHOLOGICAL EVENTS
KR100382703B1 (en) * 2000-03-15 2003-05-09 주식회사 바이오메드랩 diagnosis kit for genotyping of Human Papillomavirus and manufacturing method for thereof
JP2004500867A (en) * 2000-06-07 2004-01-15 ベイラー　カレッジ　オブ　メディシン Novel compositions and methods for array-based nucleic acid hybridization
KR100420785B1 (en) * 2000-09-29 2004-03-02 한국과학기술원 Process for Preparing Probe Using Codon Scanning Algorithm
ZA200303132B (en) * 2000-10-20 2004-09-23 Expression Diagnostics Inc Leukocyte expression profiling.
US6861214B1 (en) 2000-10-23 2005-03-01 Beckman Coulter, Inc. Immobilization of biopolymers to aminated substrates by direct adsorption
US6500921B1 (en) * 2000-11-07 2002-12-31 Amersham Biosciences Ab Schiff base reductant co-dispense process
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
WO2003016574A1 (en) * 2001-08-16 2003-02-27 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
AU2002367886B8 (en) * 2001-10-12 2008-08-14 Perkinelmer Las, Inc. Compilations of nucleic acids and arrays and methods of using them
WO2003042697A1 (en) * 2001-11-14 2003-05-22 Genospectra, Inc. Biochemical analysis system with combinatorial chemistry applications
US20030124542A1 (en) * 2001-12-28 2003-07-03 Spectral Genomics, Inc. Methods for mapping the chromosomal loci of genes expressed by a cell
US6987180B2 (en) * 2002-02-07 2006-01-17 President And Fellows Of Harvard College Uridine analogs and techniques for making and using
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20070248978A1 (en) * 2006-04-07 2007-10-25 Expression Diagnostics, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US20060263813A1 (en) * 2005-05-11 2006-11-23 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20070092886A1 (en) * 2005-03-22 2007-04-26 Raymond Tabibiazar Methods and compositions for diagnosis, monitoring and development of therapeutics for treatment of atherosclerotic disease
CN1908189A (en) * 2005-08-02 2007-02-07 博奥生物有限公司 Method of external assistant identifying intestinal-type gastric cancer and differentiation degree thereof and special reagent case
DE102005039726B3 (en) * 2005-08-19 2007-01-11 Universität Rostock Detecting nucleic acid sequence, comprises hybridizing single stranded target strand with protected strand, reacting part of strand with redoxmarker, hybridizing surface with immobilized probe and hybridizing by electro analytical process
WO2008021431A2 (en) * 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) * 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
AR084757A1 (en) * 2010-12-30 2013-06-05 Lfb Biotechnologies PROCEDURE FOR IMMOBILIZATION OF NUCLEIC LIGANDS
US10100092B2 (en) 2011-11-03 2018-10-16 Vca, Inc. Compositions and methods to detect various infectious organisms
JP6408380B2 (en) 2011-12-19 2018-10-17 ヴァリー ヘルス システム Method and kit for diagnosing a subject at risk of having cancer
EP3182126A3 (en) 2012-06-15 2017-08-02 Wayne State University Biomarker test for prediction or early detection of preeclampsia and/or hellp syndrome
EP3339861B8 (en) 2012-06-15 2023-11-01 Genesis Theranostix Korlatolt Felelossegu Tarsasag Biomarker test for prediction or early detection of preeclampsia
AU2013302861A1 (en) 2012-08-13 2015-03-05 The Rockefeller University Treatment and diagnosis of melanoma
JP6109951B2 (en) 2012-10-31 2017-04-05 ザ ロックフェラー ユニバーシティー Treatment and diagnosis of colon cancer
US20160370356A1 (en) * 2014-02-24 2016-12-22 President And Fellows Of Harvard College Paper-based immunoassay with polymerization-based signal amplification
CN106795557A (en) 2014-06-02 2017-05-31 瓦利保健系统公司 For the method and system of pulmonary cancer diagnosis
FR3035794B1 (en) 2015-05-06 2017-05-05 Elicityl PROCESS FOR THE PURIFICATION OF TOTAL BLOOD OR A PRODUCT FROM BLOOD
FR3035799B1 (en) 2015-05-06 2017-05-05 Elicityl SUPPORT FOR THE PURIFICATION OF BIOLOGICAL LIQUIDS
CA3078981A1 (en) 2017-11-21 2019-05-31 Rgenix, Inc. Polymorphs and uses thereof
US11884980B2 (en) 2018-11-28 2024-01-30 Bioscreening & Diagnostics Llc Method for detection of traumatic brain injury
AU2020401286A1 (en) 2019-12-13 2022-06-23 Inspirna, Inc. Metal salts and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE361631C (en) * 1922-10-17 Karl Kramb Row chair for theaters, cinemas and concert halls
US3594037A (en) * 1970-04-09 1971-07-20 Mc Donnell Douglas Corp Cabin attendant seat
US4423208A (en) * 1982-02-24 1983-12-27 Peter Grandics Affinity gel-adsorbent
US4687808A (en) * 1982-08-12 1987-08-18 Biospecific Technologies, Inc. Activation of biocompatible polymers with biologicals whose binding complements are pathological effectors
IT1218348B (en) * 1983-05-13 1990-04-12 Farmaceutico Lofarma S A S Ora METHOD FOR STABLY LINKING ANTIGENTS AND ALLERGENS TO SOLID SUPPORTS
US4549011A (en) * 1983-09-19 1985-10-22 Orgenics Ltd. Modified sheet of material and method of making and using same in connection with biochemical procedures
JPS61501746A (en) * 1984-04-05 1986-08-21 ライフ　テクノロジ−ズ，インコ−ポレイテツド Nucleic acid immobilization method
FR2567523B1 (en) * 1984-07-12 1987-11-13 Pasteur Institut COVALENTLY BONDED POLYNUCLEOTIDES TO A SOLID SUPPORT, AND METHOD FOR THEIR PRODUCTION
JPS622164A (en) * 1985-06-25 1987-01-08 シスカ・ダイアグノステイツクス・インコ−ポレ−テツド Nucleic acid probe containing n4-(substituted amino) cytidine
DE8521459U1 (en) * 1985-07-25 1985-09-19 Hohenloher Spezialmöbelwerk Schaffitzel GmbH + Co, 7110 Öhringen Lecture hall chairs
JP2641880B2 (en) * 1986-08-11 1997-08-20 シスカ・ダイアグノスティックス・インコーポレーテッド Nucleic acid probe assays and compositions
GB2197720A (en) * 1986-11-20 1988-05-25 Nat Res Dev Immobilisation of polynucleotides
US4824870A (en) * 1987-09-14 1989-04-25 Gelman Sciences, Inc. Polyaldehyde activated membranes

Also Published As

Publication number Publication date
DE69029717D1 (en) 1997-02-27
US5215882A (en) 1993-06-01
GR900100828A (en) 1992-04-17
GR1001043B (en) 1993-03-31
CA2031026A1 (en) 1991-05-31
DE69029717T2 (en) 1997-06-05
HK95097A (en) 1997-08-01
EP0435470B1 (en) 1997-01-15
EP0435470A1 (en) 1991-07-03

Similar Documents

Publication Publication Date Title
ATE147860T1 (en) METHOD FOR IMMOBILIZING NUCLEIC ACID TO A SOLID SURFACE FOR USE IN NUCLEIC ACID HYBRIDIZATION TESTS
DE3689412D1 (en) METHOD FOR CARRYING OUT HYBRIDIZATION TESTS OF NUCLEIC ACIDS.
DE68915202T2 (en) Methods for the propagation and detection of nucleic acid sequences.
DE3784374T2 (en) METHOD FOR RECOVERY OF HYDROCARBON DAMPERS.
DE68907407T2 (en) METHOD FOR SEQUENCE ANALYSIS OF DNA.
ATA376785A (en) METHOD FOR IDENTIFYING NUCLEIC ACIDS
DE69003104T2 (en) METHOD FOR DETECTING SPECIFIC SEQUENCES OF NUCLEIC ACIDS AND THEIR USE.
DE3774360D1 (en) METHOD FOR THE HYDRORAFFINATION OF HYDROCARBON USED.
DE3879253T2 (en) METHOD FOR AMMOXIDATION AND CATALYST COMPOSITION THEREFOR.
DE3882075D1 (en) METHOD FOR AMMOXIDATION AND CATALYST COMPOSITION THEREFOR.
DE3880857D1 (en) CATALYST COMPOSITIONS AND METHOD FOR COPOLYMERIZING CO WITH ONE OR MORE UNSATURATED OLEFINIC COMPOUNDS.
DE69231400D1 (en) Method of distinguishing nucleic acid base types
DE68914372D1 (en) Methods and equipment for identifying DNA bases.
DE3688965D1 (en) METHOD AND STRUCTURE OF A NUCLEIC ACID FOR GENERATING REAGENT COMPLEXES FOR USE IN DETERMINING TARGET NUCLEOTIDE SEQUENCES.
DE68906768D1 (en) METHOD AND SYSTEM FOR THE PRODUCTION OF CURVED HARDENED AUTOMOTIVE DISC.
DE69030804T2 (en) REAGENTS FOR IMPROVING HYBRIDIZATION
DE3765881D1 (en) METHOD FOR POLYMERIZING ALPHA OLEFINS.
DE68910096T2 (en) Method and device for forming letter outlines.
DE3685815D1 (en) METHOD FOR IDENTIFYING LYMPHOCYT SUBPOPULATIONS COLORED WITH POLYMETHINE, AND COMPOSITIONS THEREOF.
DE68917116T3 (en) Process for the production of steel sheet with excellent deep drawability.
DE3881551D1 (en) METHOD FOR THE AMMONOXIDATION OF PARAFFINS AND CATALYST THEREFOR.
ATE98701T1 (en) PROCEDURE FOR PERFORMING HYBRIDIZATION TESTS OF NUCLEIC ACIDS.
DE3675905D1 (en) METHOD FOR THE BIOLOGICAL DENITRIFICATION OF WATER AND DEVICE FOR CARRYING OUT THIS METHOD.
ATE118823T1 (en) RAPID METHOD FOR IDENTIFYING A SPECIFIC SEQUENCE OF NUCLEIC ACID.
DE58906290D1 (en) Methods of identifying ships.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties