AR039501A1 - N-ACETIL TRANSFERASE GLYPHOSATE GENES (GAT) - Google Patents

N-ACETIL TRANSFERASE GLYPHOSATE GENES (GAT)

Info

Publication number
AR039501A1
AR039501A1 ARP030101509A ARP030101509A AR039501A1 AR 039501 A1 AR039501 A1 AR 039501A1 AR P030101509 A ARP030101509 A AR P030101509A AR P030101509 A ARP030101509 A AR P030101509A AR 039501 A1 AR039501 A1 AR 039501A1
Authority
AR
Argentina
Prior art keywords
new
proteins
glyphosate
compounds
seq
Prior art date
Application number
ARP030101509A
Other languages
Spanish (es)
Inventor
Dan Siehl
Lorraine J Giver
Jeremy Minshull
Christina Ivy
Yong Hong Chen
Phillip A Patten
Rebecca Gorton
Nicholas B Duck
Billy F Mccutchen
Roger Kemble
Linda A Castle
Original Assignee
Verdia Inc
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verdia Inc, Pioneer Hi Bred Int filed Critical Verdia Inc
Publication of AR039501A1 publication Critical patent/AR039501A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Abstract

La presente brinda nuevas proteínas, que incluyen proteínas capaces de catalizar la acetilación de glifosato y otras proteínas estructuralmente relacionadas. Asimismo, la presente brinda nuevos polinucleótidos capaces de codificar estas proteínas, composiciones que incluyen una o más de estas nuevas proteínas y/o polinucleótidos, células recombinantes y plantas transgénicas que comprenden estos nuevos compuestos, métodos de diversificación que comprenden los nuevos compuestos, y métodos para utilizar los compuestos. Algunos de los nuevos métodos y compuestos que brinda la presente pueden utilizarse para tornar un organismo, como ser una planta, resistente al glifosato. Reivindicación 1: Un polinucleótido aislado o recombinante que comprende: (a) una secuencia de nucleótidos que codifica una secuencia de aminoácidos que puede estar óptimamente alineada con una secuencia seleccionada entre el grupo que comprende SEQ ID N°: 300, SEQ ID N°: 445 y SEQ ID N°: 457 para generar una puntuación de similitud al menos de 430, utilizando la matriz BLOSUM62, una penalización de existencia de espacios de 11 y una penalización de extensión de espacios de 1; o (b) una secuencia de nucleótidos complementaria de la misma.This provides new proteins, which include proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Likewise, the present provides new polynucleotides capable of encoding these proteins, compositions that include one or more of these new proteins and / or polynucleotides, recombinant cells and transgenic plants comprising these new compounds, diversification methods comprising the new compounds, and methods To use the compounds. Some of the new methods and compounds provided by this can be used to make an organism, such as a plant, resistant to glyphosate. Claim 1: An isolated or recombinant polynucleotide comprising: (a) a nucleotide sequence encoding an amino acid sequence that may be optimally aligned with a sequence selected from the group comprising SEQ ID N °: 300, SEQ ID N °: 445 and SEQ ID N °: 457 to generate a similarity score of at least 430, using the BLOSUM62 matrix, a penalty of the existence of spaces of 11 and a penalty of extension of spaces of 1; or (b) a complementary nucleotide sequence thereof.

ARP030101509A 2002-04-30 2003-04-30 N-ACETIL TRANSFERASE GLYPHOSATE GENES (GAT) AR039501A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37771902P 2002-04-30 2002-04-30
US37717502P 2002-05-01 2002-05-01

Publications (1)

Publication Number Publication Date
AR039501A1 true AR039501A1 (en) 2005-02-23

Family

ID=29406784

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP030101509A AR039501A1 (en) 2002-04-30 2003-04-30 N-ACETIL TRANSFERASE GLYPHOSATE GENES (GAT)
ARP080105196A AR069485A2 (en) 2002-04-30 2008-11-28 GLYPHOSATE-N-ACETYLTRANSPHERASE (GAT) GENES

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP080105196A AR069485A2 (en) 2002-04-30 2008-11-28 GLYPHOSATE-N-ACETYLTRANSPHERASE (GAT) GENES

Country Status (4)

Country Link
AR (2) AR039501A1 (en)
AU (1) AU2003234328A1 (en)
CL (2) CL2009000280A1 (en)
WO (1) WO2003092360A2 (en)

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