ZA202308025B - Gene transcription framework, vector system, genome sequence editing method and application - Google Patents

Gene transcription framework, vector system, genome sequence editing method and application

Info

Publication number
ZA202308025B
ZA202308025B ZA2023/08025A ZA202308025A ZA202308025B ZA 202308025 B ZA202308025 B ZA 202308025B ZA 2023/08025 A ZA2023/08025 A ZA 2023/08025A ZA 202308025 A ZA202308025 A ZA 202308025A ZA 202308025 B ZA202308025 B ZA 202308025B
Authority
ZA
South Africa
Prior art keywords
sequence
framework
editing method
genome
gene transcription
Prior art date
Application number
ZA2023/08025A
Other languages
English (en)
Inventor
Shuanghong Peng
Yunpeng Sui
Original Assignee
Shuanghong Peng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shuanghong Peng filed Critical Shuanghong Peng
Publication of ZA202308025B publication Critical patent/ZA202308025B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Hospice & Palliative Care (AREA)
  • Epidemiology (AREA)
  • Psychiatry (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Psychology (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Management Or Editing Of Information On Record Carriers (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ZA2023/08025A 2021-01-22 2023-08-18 Gene transcription framework, vector system, genome sequence editing method and application ZA202308025B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110089068.1A CN112708636A (zh) 2021-01-22 2021-01-22 基因转录框架、载体系统、基因组序列编辑方法及应用
PCT/CN2021/134710 WO2022156378A1 (zh) 2021-01-22 2021-12-01 基因转录框架、载体系统、基因组序列编辑方法及应用

Publications (1)

Publication Number Publication Date
ZA202308025B true ZA202308025B (en) 2024-03-27

Family

ID=75550451

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2023/08025A ZA202308025B (en) 2021-01-22 2023-08-18 Gene transcription framework, vector system, genome sequence editing method and application

Country Status (11)

Country Link
US (1) US20250263737A1 (https=)
EP (1) EP4282968A4 (https=)
JP (1) JP2024504592A (https=)
KR (1) KR20230135630A (https=)
CN (1) CN112708636A (https=)
AU (1) AU2021422067A1 (https=)
BR (1) BR112023014382A2 (https=)
CA (1) CA3206081A1 (https=)
MX (1) MX2023008635A (https=)
WO (1) WO2022156378A1 (https=)
ZA (1) ZA202308025B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112481259B (zh) * 2020-11-24 2022-09-16 南昌大学 两种甘薯U6基因启动子IbU6的克隆与应用
CN115044583A (zh) * 2022-03-21 2022-09-13 隋云鹏 用于基因编辑的rna框架和基因编辑方法
CN115312121B (zh) * 2022-09-29 2023-03-24 北京齐碳科技有限公司 靶基因位点检测方法、装置、设备及计算机存储介质

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2281050B1 (en) * 2008-04-14 2014-04-02 Sangamo BioSciences, Inc. Linear donor constructs for targeted integration
CN102080101B (zh) * 2010-11-25 2013-01-09 北京济福霖生物技术有限公司 一种定点整合外源基因的方法
WO2016030501A1 (en) * 2014-08-28 2016-03-03 Centre National De La Recherche Scientifique - Cnrs - Synthetic alu-retrotransposon vectors for gene therapy
US11339427B2 (en) * 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
CN113286880A (zh) * 2018-08-28 2021-08-20 旗舰先锋创新Vi有限责任公司 调控基因组的方法和组合物
US20210340562A1 (en) * 2018-10-19 2021-11-04 Board Of Regents, The University Of Texas System Engineered long interspersed element (line) transposons and methods of use
JP7831937B2 (ja) * 2019-09-03 2026-03-17 クリエイト・メディシンズ，インコーポレーテッド ゲノム組込みのための方法および組成物
CN112210573B (zh) * 2020-10-14 2024-02-06 浙江大学 一种用基因编辑改造原代细胞的dna模板及定点插入方法

Also Published As

Publication number Publication date
EP4282968A4 (en) 2024-12-04
JP2024504592A (ja) 2024-02-01
BR112023014382A2 (pt) 2023-10-31
CN112708636A (zh) 2021-04-27
US20250263737A1 (en) 2025-08-21
CA3206081A1 (en) 2022-07-28
AU2021422067A1 (en) 2023-09-07
KR20230135630A (ko) 2023-09-25
MX2023008635A (es) 2023-10-17
EP4282968A1 (en) 2023-11-29
WO2022156378A1 (zh) 2022-07-28

Similar Documents

Publication Publication Date Title
ZA202308025B (en) Gene transcription framework, vector system, genome sequence editing method and application
Cullot et al. Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations
Wienert et al. In vitro–transcribed guide RNAs trigger an innate immune response via the RIG-I pathway
US10760081B2 (en) Compositions and methods for enhancing CRISPR activity by POLQ inhibition
Del Campo et al. Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function
Howden et al. A Cas9 variant for efficient generation of indel-free knockin or gene-corrected human pluripotent stem cells
Hartenian et al. Feedback to the central dogma: cytoplasmic mRNA decay and transcription are interdependent processes
CA2954791C (en) CRISPR/CAS TRANSCRIPTIONAL MODULATION
Subtelny et al. Poly (A)-tail profiling reveals an embryonic switch in translational control
Standage-Beier et al. A transient reporter for editing enrichment (TREE) in human cells
Nguyen et al. The internal loops in the lower stem of primary microRNA transcripts facilitate single cleavage of human Microprocessor
Joberty et al. A tandem guide RNA-based strategy for efficient CRISPR gene editing of cell populations with low heterogeneity of edited alleles
Cervera et al. Therapeutic potential of ewsr1–fli1 inactivation by crispr/cas9 in ewing sarcoma
Cheng et al. CRISPR/Cas9-mediated chicken TBK1 gene knockout and its essential role in STING-mediated IFN-β induction in chicken cells
Daume et al. RIP-Seq suggests translational regulation by L7Ae in Archaea
US20250376677A1 (en) Type i crispr system as a tool for genome editing
Hwang et al. Precise editing of pathogenic nucleotide repeat expansions in iPSCs using paired prime editor
Shen et al. Self-splicing RNA circularization facilitated by intact group I and II introns
Yu et al. Circle-seq based method for eccDNA synthesis and its application as a canonical promoter independent vector for robust microRNA overexpression
CN110684736A (zh) 一种基于CRISPR-Cas9编辑技术的敲除鸡Shp-2基因的细胞系及其构建方法
Shang et al. The destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
Spiewla et al. PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA
Choi et al. Development of a rapid, simple and efficient one-pot cloning method for a reverse genetics system of broad subtypes of influenza A virus
US20250136961A1 (en) Isolated nuclease and use thereof
KR102667508B1 (ko) 프라임 에디팅 시스템을 이용한 게놈 편집의 과정에서 발생 가능한 오프 타겟을 예측하는 방법