ZA200308519B - Method of colorimetry and reagent for use therein. - Google Patents
Method of colorimetry and reagent for use therein. Download PDFInfo
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- ZA200308519B ZA200308519B ZA200308519A ZA200308519A ZA200308519B ZA 200308519 B ZA200308519 B ZA 200308519B ZA 200308519 A ZA200308519 A ZA 200308519A ZA 200308519 A ZA200308519 A ZA 200308519A ZA 200308519 B ZA200308519 B ZA 200308519B
- Authority
- ZA
- South Africa
- Prior art keywords
- group
- compound
- complex
- reagent
- colorimetric method
- Prior art date
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 141
- 238000004737 colorimetric analysis Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title abstract description 8
- 238000004040 coloring Methods 0.000 claims abstract description 58
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 20
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 20
- 239000012491 analyte Substances 0.000 claims abstract description 12
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000001301 oxygen Substances 0.000 claims abstract description 8
- 238000004451 qualitative analysis Methods 0.000 claims abstract description 4
- 238000004445 quantitative analysis Methods 0.000 claims abstract description 4
- -1 bipyridyl compound Chemical class 0.000 claims description 99
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 78
- 239000003446 ligand Substances 0.000 claims description 54
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 52
- 239000008103 glucose Substances 0.000 claims description 48
- 125000001424 substituent group Chemical group 0.000 claims description 31
- 229910052762 osmium Inorganic materials 0.000 claims description 23
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 21
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 21
- 150000001875 compounds Chemical class 0.000 claims description 21
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 claims description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 150000001413 amino acids Chemical class 0.000 claims description 17
- 150000004699 copper complex Chemical class 0.000 claims description 16
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 14
- 239000012327 Ruthenium complex Substances 0.000 claims description 13
- 125000003118 aryl group Chemical group 0.000 claims description 12
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 12
- 125000005843 halogen group Chemical group 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 12
- 125000001174 sulfone group Chemical group 0.000 claims description 12
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 claims description 11
- 125000002252 acyl group Chemical group 0.000 claims description 11
- 125000003545 alkoxy group Chemical group 0.000 claims description 11
- 125000000217 alkyl group Chemical group 0.000 claims description 11
- 125000003368 amide group Chemical group 0.000 claims description 11
- 125000003277 amino group Chemical group 0.000 claims description 11
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims description 11
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 11
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 11
- 125000000018 nitroso group Chemical group N(=O)* 0.000 claims description 11
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 11
- 150000003141 primary amines Chemical class 0.000 claims description 11
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 claims description 11
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 claims description 11
- 150000003512 tertiary amines Chemical class 0.000 claims description 11
- XAZMYOQLNVTRPS-UHFFFAOYSA-L disodium;2-[[4-[2-(1,3-benzothiazol-2-yl)-3-[4-[4-[3-(1,3-benzothiazol-2-yl)-5-[4-[bis(2-sulfonatoethyl)carbamoyl]phenyl]tetrazol-3-ium-2-yl]-3-methoxyphenyl]-2-methoxyphenyl]tetrazol-2-ium-5-yl]benzoyl]-(2-sulfonatoethyl)amino]ethanesulfonate Chemical compound [Na+].[Na+].COC1=CC(C=2C=C(OC)C(=CC=2)N2[N+](=NC(=N2)C=2C=CC(=CC=2)C(=O)N(CCS([O-])(=O)=O)CCS([O-])(=O)=O)C=2SC3=CC=CC=C3N=2)=CC=C1N([N+](=N1)C=2SC3=CC=CC=C3N=2)N=C1C1=CC=C(C(=O)N(CCS([O-])(=O)=O)CCS([O-])(=O)=O)C=C1 XAZMYOQLNVTRPS-UHFFFAOYSA-L 0.000 claims description 10
- 150000004698 iron complex Chemical class 0.000 claims description 9
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 9
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Natural products CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 claims description 7
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- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 claims description 7
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- 229940107161 cholesterol Drugs 0.000 claims description 7
- IOJUPLGTWVMSFF-UHFFFAOYSA-N cyclobenzothiazole Natural products C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 claims description 7
- QDLAGTHXVHQKRE-UHFFFAOYSA-N lichenxanthone Natural products COC1=CC(O)=C2C(=O)C3=C(C)C=C(OC)C=C3OC2=C1 QDLAGTHXVHQKRE-UHFFFAOYSA-N 0.000 claims description 7
- 150000002832 nitroso derivatives Chemical class 0.000 claims description 7
- 235000006408 oxalic acid Nutrition 0.000 claims description 7
- 101710088194 Dehydrogenase Proteins 0.000 claims description 6
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims description 6
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 claims description 6
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 claims description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- VSIVTUIKYVGDCX-UHFFFAOYSA-M sodium;4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].COC1=CC([N+]([O-])=O)=CC=C1[N+]1=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=NN1C1=CC=C([N+]([O-])=O)C=C1 VSIVTUIKYVGDCX-UHFFFAOYSA-M 0.000 claims description 5
- YBCKCPQYXKLDQB-UHFFFAOYSA-N 2-(2,5-diphenyl-1h-tetrazol-1-ium-3-yl)-1,3-benzothiazole;bromide Chemical compound [Br-].[NH2+]1N(C=2SC3=CC=CC=C3N=2)N(C=2C=CC=CC=2)N=C1C1=CC=CC=C1 YBCKCPQYXKLDQB-UHFFFAOYSA-N 0.000 claims description 4
- QCMGHDUWICBHPO-UHFFFAOYSA-N 2-[3-(4-nitrophenyl)-5-phenyl-1h-tetrazol-1-ium-2-yl]-1,3-benzothiazole;bromide Chemical compound [Br-].C1=CC([N+](=O)[O-])=CC=C1N1N(C=2SC3=CC=CC=C3N=2)[NH2+]C(C=2C=CC=CC=2)=N1 QCMGHDUWICBHPO-UHFFFAOYSA-N 0.000 claims description 4
- VVTIATCVLPUOPW-UHFFFAOYSA-N 4-[(4-ethylpiperazin-1-yl)methyl]aniline Chemical compound C1CN(CC)CCN1CC1=CC=C(N)C=C1 VVTIATCVLPUOPW-UHFFFAOYSA-N 0.000 claims description 4
- HYIKCRFBXGSHOM-UHFFFAOYSA-N 5-methyl-2-(3-nitrophenyl)-3-phenyl-1h-tetrazol-1-ium;chloride Chemical compound [Cl-].[NH2+]1C(C)=NN(C=2C=C(C=CC=2)[N+]([O-])=O)N1C1=CC=CC=C1 HYIKCRFBXGSHOM-UHFFFAOYSA-N 0.000 claims description 4
- IKQYOSGGDFNMHI-UHFFFAOYSA-N 5-methyl-2-(4-nitrophenyl)-3-phenyl-1h-tetrazol-1-ium;chloride Chemical compound [Cl-].[NH2+]1C(C)=NN(C=2C=CC(=CC=2)[N+]([O-])=O)N1C1=CC=CC=C1 IKQYOSGGDFNMHI-UHFFFAOYSA-N 0.000 claims description 4
- 125000004429 atom Chemical group 0.000 claims description 4
- JUJBNYBVVQSIOU-UHFFFAOYSA-M sodium;4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 JUJBNYBVVQSIOU-UHFFFAOYSA-M 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims description 3
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- 125000001164 benzothiazolyl group Chemical group S1C(=NC2=C1C=CC=C2)* 0.000 claims description 3
- 229960003624 creatine Drugs 0.000 claims description 3
- 239000006046 creatine Substances 0.000 claims description 3
- 229940109239 creatinine Drugs 0.000 claims description 3
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- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 3
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- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims 1
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- 238000006243 chemical reaction Methods 0.000 abstract description 18
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- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 8
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- RJDOZRNNYVAULJ-UHFFFAOYSA-L [O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[F-].[F-].[Mg++].[Mg++].[Mg++].[Al+3].[Si+4].[Si+4].[Si+4].[K+] Chemical compound [O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[F-].[F-].[Mg++].[Mg++].[Mg++].[Al+3].[Si+4].[Si+4].[Si+4].[K+] RJDOZRNNYVAULJ-UHFFFAOYSA-L 0.000 description 2
- 239000002280 amphoteric surfactant Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 235000010290 biphenyl Nutrition 0.000 description 2
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- 239000008280 blood Substances 0.000 description 2
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 2
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- 239000004744 fabric Substances 0.000 description 2
- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine hydrate Chemical compound O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 2
- 239000010445 mica Substances 0.000 description 2
- 229910052618 mica group Inorganic materials 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- SBWGZAXBCCNRTM-CTHBEMJXSA-N n-methyl-n-[(2s,3r,4r,5r)-2,3,4,5,6-pentahydroxyhexyl]octanamide Chemical compound CCCCCCCC(=O)N(C)C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO SBWGZAXBCCNRTM-CTHBEMJXSA-N 0.000 description 2
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
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- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000012088 reference solution Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- ZLLMYPVFZJLNKV-UHFFFAOYSA-M sodium 3-[3-hydroxy-4-[(5-nitropyridin-2-yl)diazenyl]-N-propylanilino]propane-1-sulfonate Chemical compound [Na+].CCCN(CCCS([O-])(=O)=O)c1ccc(N=Nc2ccc(cn2)[N+]([O-])=O)c(O)c1 ZLLMYPVFZJLNKV-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- WSNJABVSHLCCOX-UHFFFAOYSA-J trilithium;trimagnesium;trisodium;dioxido(oxo)silane;tetrafluoride Chemical compound [Li+].[Li+].[Li+].[F-].[F-].[F-].[F-].[Na+].[Na+].[Na+].[Mg+2].[Mg+2].[Mg+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O WSNJABVSHLCCOX-UHFFFAOYSA-J 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical class C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 description 1
- VXQPHDBRWYMWKH-UHFFFAOYSA-M 1,2-diphenyltetrazol-1-ium;bromide Chemical compound [Br-].C=1C=CC=CC=1[N+]1=CN=NN1C1=CC=CC=C1 VXQPHDBRWYMWKH-UHFFFAOYSA-M 0.000 description 1
- VYMPLPIFKRHAAC-UHFFFAOYSA-N 1,2-ethanedithiol Chemical compound SCCS VYMPLPIFKRHAAC-UHFFFAOYSA-N 0.000 description 1
- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
- LZEZZYPQKOSMQJ-UHFFFAOYSA-N 1h-imidazole;iron Chemical compound [Fe].C1=CNC=N1 LZEZZYPQKOSMQJ-UHFFFAOYSA-N 0.000 description 1
- QKUFVAFAVFCMBS-UHFFFAOYSA-N 1h-imidazole;osmium Chemical compound [Os].C1=CNC=N1 QKUFVAFAVFCMBS-UHFFFAOYSA-N 0.000 description 1
- YLJKGLUZYGCARD-UHFFFAOYSA-N 1h-imidazole;ruthenium Chemical compound [Ru].C1=CNC=N1 YLJKGLUZYGCARD-UHFFFAOYSA-N 0.000 description 1
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 1
- POPSGTLBIDPIDC-UHFFFAOYSA-N 2,3-bis(4-nitrophenyl)-1h-tetrazol-1-ium;perchlorate Chemical compound [O-]Cl(=O)(=O)=O.C1=CC([N+](=O)[O-])=CC=C1N1N(C=2C=CC(=CC=2)[N+]([O-])=O)N=C[NH2+]1 POPSGTLBIDPIDC-UHFFFAOYSA-N 0.000 description 1
- NSMJMUQZRGZMQC-UHFFFAOYSA-N 2-naphthalen-1-yl-1H-imidazo[4,5-f][1,10]phenanthroline Chemical compound C12=CC=CN=C2C2=NC=CC=C2C2=C1NC(C=1C3=CC=CC=C3C=CC=1)=N2 NSMJMUQZRGZMQC-UHFFFAOYSA-N 0.000 description 1
- INWFLBXPENDFJW-UHFFFAOYSA-N 3,4-dimethyl-2-pyridin-2-ylpyridine Chemical group CC1=CC=NC(C=2N=CC=CC=2)=C1C INWFLBXPENDFJW-UHFFFAOYSA-N 0.000 description 1
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
- BQGXXEGJJMEZMZ-UHFFFAOYSA-N 3-(n-ethyl-3-hydroxy-4-nitrosoanilino)propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN(CC)C1=CC=C(N=O)C(O)=C1 BQGXXEGJJMEZMZ-UHFFFAOYSA-N 0.000 description 1
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- DSOBYSHRIJISFE-UHFFFAOYSA-N 3-[3-hydroxy-4-[(5-nitropyridin-2-yl)diazenyl]-N-propylanilino]propane-1-sulfonic acid Chemical compound OC1=CC(N(CCCS(O)(=O)=O)CCC)=CC=C1N=NC1=CC=C([N+]([O-])=O)C=N1 DSOBYSHRIJISFE-UHFFFAOYSA-N 0.000 description 1
- QZHPOWUOLXLXGX-UHFFFAOYSA-N 4-[2-(1,3-benzothiazol-2-yl)-5-[4-(2-sulfoethylcarbamoyl)phenyl]-1h-tetrazol-3-yl]-3-methoxybenzoic acid Chemical compound COC1=CC(C(O)=O)=CC=C1N1N(C=2SC3=CC=CC=C3N=2)NC(C=2C=CC(=CC=2)C(=O)NCCS(O)(=O)=O)=N1 QZHPOWUOLXLXGX-UHFFFAOYSA-N 0.000 description 1
- DBOOMZAMPJBDOE-UHFFFAOYSA-N 4-[2-(2,4-dinitrophenyl)-3-(4-iodophenyl)-1h-tetrazol-5-yl]benzene-1,3-disulfonic acid Chemical compound OS(=O)(=O)C1=CC(S(=O)(=O)O)=CC=C1C1=NN(C=2C=CC(I)=CC=2)N(C=2C(=CC(=CC=2)[N+]([O-])=O)[N+]([O-])=O)N1 DBOOMZAMPJBDOE-UHFFFAOYSA-N 0.000 description 1
- KIIHBDSNVJRWFY-UHFFFAOYSA-N 4-bromo-2-(4-bromopyridin-2-yl)pyridine Chemical group BrC1=CC=NC(C=2N=CC=C(Br)C=2)=C1 KIIHBDSNVJRWFY-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 1
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000006173 Good's buffer Substances 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 229910019891 RuCl3 Inorganic materials 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- YBCVMFKXIKNREZ-UHFFFAOYSA-N acoh acetic acid Chemical compound CC(O)=O.CC(O)=O YBCVMFKXIKNREZ-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
- QQACEAMJCJRFPS-UHFFFAOYSA-N copper;1h-imidazole;2-pyridin-2-ylpyridine Chemical compound [Cu].C1=CNC=N1.N1=CC=CC=C1C1=CC=CC=N1 QQACEAMJCJRFPS-UHFFFAOYSA-N 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- JFVUMQYWYNDZDK-UHFFFAOYSA-N ferrozine free acid Chemical compound C1=CC(S(=O)(=O)O)=CC=C1C1=NN=C(C=2N=CC=CC=2)N=C1C1=CC=C(S(O)(=O)=O)C=C1 JFVUMQYWYNDZDK-UHFFFAOYSA-N 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 235000012209 glucono delta-lactone Nutrition 0.000 description 1
- 229960003681 gluconolactone Drugs 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- UKVIEHSSVKSQBA-UHFFFAOYSA-N methane;palladium Chemical compound C.[Pd] UKVIEHSSVKSQBA-UHFFFAOYSA-N 0.000 description 1
- 150000002907 osmium Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010405 reoxidation reaction Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 150000003303 ruthenium Chemical class 0.000 description 1
- 229910052707 ruthenium Inorganic materials 0.000 description 1
- YBCAZPLXEGKKFM-UHFFFAOYSA-K ruthenium(iii) chloride Chemical compound [Cl-].[Cl-].[Cl-].[Ru+3] YBCAZPLXEGKKFM-UHFFFAOYSA-K 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910000275 saponite Inorganic materials 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- AZJYLVAUMGUUBL-UHFFFAOYSA-A u1qj22mc8e Chemical compound [F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[F-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].O=[Si]=O.O=[Si]=O.O=[Si]=O.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3 AZJYLVAUMGUUBL-UHFFFAOYSA-A 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plasma & Fusion (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Plural Heterocyclic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Spectrometry And Color Measurement (AREA)
- Cosmetics (AREA)
Applications Claiming Priority (1)
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JP2001400380 | 2001-12-28 |
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ZA200308519A ZA200308519B (en) | 2001-12-28 | 2003-10-31 | Method of colorimetry and reagent for use therein. |
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US (2) | US7550273B2 (fr) |
EP (2) | EP1942195B1 (fr) |
JP (1) | JP4045323B2 (fr) |
KR (2) | KR100693899B1 (fr) |
CN (1) | CN1261588C (fr) |
AT (2) | ATE482285T1 (fr) |
AU (1) | AU2003201901B2 (fr) |
CA (1) | CA2448128C (fr) |
DE (2) | DE60334333D1 (fr) |
ES (2) | ES2350507T3 (fr) |
NZ (1) | NZ529279A (fr) |
PL (1) | PL213316B1 (fr) |
WO (1) | WO2003057905A1 (fr) |
ZA (1) | ZA200308519B (fr) |
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DE60334333D1 (de) | 2001-12-28 | 2010-11-04 | Arkray Inc | Kolorimetrisches Verfahren und dafür verwendetes Reagens |
CN1306039C (zh) * | 2001-12-28 | 2007-03-21 | 爱科来株式会社 | 比色分析方法及其使用的试剂 |
WO2005066638A1 (fr) * | 2004-01-07 | 2005-07-21 | Arkray, Inc. | Instrument analytique a agencement ameliore de section de reactif et procede analytique correspondant |
EP2228645B1 (fr) * | 2008-05-16 | 2013-09-04 | Panasonic Corporation | Procédé de mesure, dispositif de mesure et appareil de mesure de la concentration en créatinine et procédé de mesure, dispositif de mesure et appareil de mesure de la quantité de salinité à l'aide de ceux-ci |
EP2636750A1 (fr) | 2012-03-06 | 2013-09-11 | Roche Diagniostics GmbH | Ectoïne en soluté compatible ainsi que ses dérivés pour la stabilisation d'enzymes |
WO2014180939A1 (fr) | 2013-05-08 | 2014-11-13 | Roche Diagnostics Gmbh | Stabilisation d'enzymes à l'aide d'acide nicotinique |
EP2927319A1 (fr) | 2014-03-31 | 2015-10-07 | Roche Diagnostics GmbH | Immobilisation d'enzyme à charge élevée par réticulation |
ES2756714T3 (es) | 2014-08-25 | 2020-04-27 | Hoffmann La Roche | Tira reactiva de dos electrodos que compensan la interferencia |
CN109804240A (zh) | 2016-10-05 | 2019-05-24 | 豪夫迈·罗氏有限公司 | 用于多分析物诊断测试元件的检测试剂和电极布置以及其使用方法 |
WO2018165180A1 (fr) | 2017-03-06 | 2018-09-13 | Medtronic Minimed, Inc. | Capteur colorimétrique pour la détection non invasive du glucose dans la sueur dans le pre-diabète et diabète de type 2 |
US20180249935A1 (en) | 2017-03-06 | 2018-09-06 | Medtronic Minimed, Inc. | Colorometric sensor for the non-invasive screening of glucose in sweat in pre and type 2 diabetes |
JP7072797B2 (ja) * | 2018-02-20 | 2022-05-23 | 公立大学法人山陽小野田市立山口東京理科大学 | 化合物及び光触媒用助触媒 |
CN116018084A (zh) | 2020-09-18 | 2023-04-25 | 美敦力迷你迈德公司 | 酮体感测装置和方法 |
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US4233402A (en) * | 1978-04-05 | 1980-11-11 | Syva Company | Reagents and method employing channeling |
JPS57192483A (en) | 1981-05-21 | 1982-11-26 | Mitsubishi Electric Corp | Electrochromic display element consisting of organic metal complex |
EP0100217B1 (fr) * | 1982-07-23 | 1987-10-07 | Wako Pure Chemical Industries, Ltd. | Procédé pour la détermination quantitative d'un substrat traité avec oxidase |
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- 2003-01-06 DE DE60334333T patent/DE60334333D1/de not_active Expired - Lifetime
- 2003-01-06 PL PL369143A patent/PL213316B1/pl unknown
- 2003-01-06 AT AT08006461T patent/ATE482285T1/de active
- 2003-01-06 CA CA002448128A patent/CA2448128C/fr not_active Expired - Fee Related
- 2003-01-06 WO PCT/JP2003/000027 patent/WO2003057905A1/fr active Application Filing
- 2003-01-06 EP EP08006461A patent/EP1942195B1/fr not_active Expired - Lifetime
- 2003-01-06 EP EP03700461A patent/EP1457572B1/fr not_active Expired - Lifetime
- 2003-01-06 KR KR1020067001158A patent/KR100693899B1/ko active IP Right Grant
- 2003-01-06 AU AU2003201901A patent/AU2003201901B2/en not_active Ceased
- 2003-01-06 DE DE60326814T patent/DE60326814D1/de not_active Expired - Lifetime
- 2003-01-06 AT AT03700461T patent/ATE426676T1/de active
- 2003-01-06 NZ NZ529279A patent/NZ529279A/en not_active IP Right Cessation
- 2003-01-06 JP JP2003558198A patent/JP4045323B2/ja not_active Expired - Lifetime
- 2003-01-06 KR KR1020037015691A patent/KR100557899B1/ko active IP Right Grant
- 2003-01-06 ES ES08006461T patent/ES2350507T3/es not_active Expired - Lifetime
- 2003-01-06 ES ES03700461T patent/ES2322990T3/es not_active Expired - Lifetime
- 2003-01-06 CN CNB038006049A patent/CN1261588C/zh not_active Expired - Lifetime
- 2003-01-06 US US10/476,298 patent/US7550273B2/en not_active Expired - Lifetime
- 2003-10-31 ZA ZA200308519A patent/ZA200308519B/en unknown
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2009
- 2009-05-20 US US12/469,403 patent/US8574896B2/en active Active
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Publication number | Publication date |
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KR100693899B1 (ko) | 2007-03-12 |
JPWO2003057905A1 (ja) | 2005-05-19 |
ES2322990T3 (es) | 2009-07-03 |
PL369143A1 (en) | 2005-04-18 |
WO2003057905A1 (fr) | 2003-07-17 |
KR20060010872A (ko) | 2006-02-02 |
ES2350507T3 (es) | 2011-01-24 |
AU2003201901B2 (en) | 2006-09-21 |
US8574896B2 (en) | 2013-11-05 |
EP1942195A1 (fr) | 2008-07-09 |
AU2003201901A1 (en) | 2003-07-24 |
EP1457572B1 (fr) | 2009-03-25 |
DE60326814D1 (de) | 2009-05-07 |
US20040142406A1 (en) | 2004-07-22 |
CN1522302A (zh) | 2004-08-18 |
NZ529279A (en) | 2005-12-23 |
US7550273B2 (en) | 2009-06-23 |
DE60334333D1 (de) | 2010-11-04 |
JP4045323B2 (ja) | 2008-02-13 |
US20090233322A1 (en) | 2009-09-17 |
CA2448128A1 (fr) | 2003-06-28 |
PL213316B1 (pl) | 2013-02-28 |
EP1457572A4 (fr) | 2006-04-05 |
EP1942195B1 (fr) | 2010-09-22 |
ATE426676T1 (de) | 2009-04-15 |
CN1261588C (zh) | 2006-06-28 |
ATE482285T1 (de) | 2010-10-15 |
EP1457572A1 (fr) | 2004-09-15 |
CA2448128C (fr) | 2009-07-14 |
KR20040010671A (ko) | 2004-01-31 |
KR100557899B1 (ko) | 2006-03-10 |
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