WO2024239299A1 - 一种用于双光子记录的脊髓夹持器 - Google Patents

一种用于双光子记录的脊髓夹持器 Download PDF

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Publication number
WO2024239299A1
WO2024239299A1 PCT/CN2023/096157 CN2023096157W WO2024239299A1 WO 2024239299 A1 WO2024239299 A1 WO 2024239299A1 CN 2023096157 W CN2023096157 W CN 2023096157W WO 2024239299 A1 WO2024239299 A1 WO 2024239299A1
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WIPO (PCT)
Prior art keywords
bracket
spinal cord
base
clamping
cord clamp
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Ceased
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PCT/CN2023/096157
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English (en)
French (fr)
Inventor
崔光富
黄天文
柯进
文善煜
钱申
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Priority to PCT/CN2023/096157 priority Critical patent/WO2024239299A1/zh
Publication of WO2024239299A1 publication Critical patent/WO2024239299A1/zh
Anticipated expiration legal-status Critical
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00—Arrangements for supporting or fettering animals for veterinary purposes

Definitions

  • the present application relates to the technical field of animal experimental equipment, and in particular to a spinal cord clamp for two-photon recording.
  • Dorsal root ganglia are important peripheral nerve tissues for humans and vertebrates to perceive peripheral stimuli, and are also an important station for transmitting peripheral sensory codes to the spinal cord. To study the neural coding mechanism of perceptions such as pain, itch, touch, cold and heat, it is necessary to study the differences in the responses of dorsal root ganglion neurons under different peripheral conditions.
  • One purpose of the present application is to provide a spinal cord clamp for two-photon recording with an adjustable clamping range, high clamping strength and high structural stability, so as to solve the technical problem that the existing spinal cord fixation equipment has poor stability and durability, resulting in low spinal cord fixation firmness, which easily causes field of view shaking and affects the imaging quality.
  • the present application provides a spinal cord clamp for two-photon recording, comprising a base and a A spinal cord clamping module of a base, the spinal cord clamping module includes a clamping member and a mounting assembly for mounting the clamping member on the base, the clamping member includes a first bracket mounted on the base and a second bracket slidably disposed on the first bracket, the first bracket and the second bracket both have clamping arms extending toward the base, and a spinal cord clamping space is formed between the clamping arms of the first bracket and the second bracket.
  • both the first bracket and the second bracket are L-shaped brackets, and the first bracket and the second bracket are connected by a structure matching screw holes and screws.
  • the first bracket includes a connecting portion for connecting to the base, two support rods extending from the connecting portion at intervals, two first clamping arms extending vertically from the corresponding support rods, and a limiting beam transversely connecting the two support rods.
  • the second bracket includes a base, two second clamping arms extending vertically from the base, and a protrusion extending protruding from the back of the base, and the protrusion is slidably disposed between two support rods.
  • both the first clamping arm and the second clamping arm are provided with tooth structures.
  • the second bracket further includes a first screw hole penetrating the base and the protruding portion, for forming a connection with the first bracket through a first screw.
  • a rectangular opening is formed between the support rods to limit the range of movement of the second bracket.
  • the first screw hole is a 2.5 mm screw hole.
  • the base includes a bottom plate and a mounting block disposed on the bottom plate, the side of the mounting block is provided with a groove for mounting the connecting portion of the first bracket and two second screw holes respectively disposed on both sides of the groove, and the mounting assembly includes a mounting assembly for mounting the first bracket.
  • the bracket is fixed to a cover plate on the mounting block and two second screws used to connect with the second screw holes to fix the cover plate on the mounting block.
  • the base plate is provided with four third screw holes for forming a connection with the two-photon microscope translation stage via screws.
  • the base is provided with a heating device.
  • the second screw hole is a 2.5 mm screw hole
  • the third screw hole is a 6 mm screw hole.
  • the spinal cord clamp of the present application can adjust the clamping range and clamping force through the slidable connection between the two brackets, which is conducive to improving the flexibility of use and the scope of application;
  • the present application provides a limited cross beam on the two support rods of the first bracket, which can ensure the stability and strength of the open end of the first bracket and ensure the clamping stability;
  • the present application provides a toothed structure on the two clamping arms, which can increase the clamping friction and ensure the firmness of the spinal cord clamp and the spine;
  • the present application provides a plurality of screw holes on the base, which can facilitate a firm connection between the spinal cord clamp and the two-photon microscope translation stage.
  • FIG1 is a schematic diagram of the three-dimensional structure of a spinal cord clamp for two-photon recording according to a preferred embodiment of the present application.
  • FIG. 2 is a schematic diagram of the three-dimensional structure of the spinal cord clamp for two-photon recording shown in FIG. 1 at another viewing angle.
  • FIG. 3 is an exploded view of the spinal cord clamp shown in FIG. 1 for two-photon recording.
  • FIG. 4 is a schematic diagram of the three-dimensional structure of the first bracket of the spinal cord clamp for two-photon recording shown in FIG. 1 .
  • FIG. 5 is a schematic diagram of the three-dimensional structure of the second bracket of the spinal cord clamp for two-photon recording shown in FIG. 1 .
  • spinal cord clamp 100 spinal cord clamp 100; base 10; bottom plate 11; third screw hole 111; mounting block 12; groove 121; second screw hole 122; spinal cord clamp module 20; clamping member 21; first bracket 211; connecting portion 2111; support rod 2112; first clamping arm 2113; limiting beam 2114; rectangular opening 2115; second bracket 212; base 2121; second clamping arm 2122; protrusion 2123; first screw hole 2124; spinal cord clamping space 213; toothed structure 214; first screw 215; mounting assembly 22; cover plate 221; second screw 222.
  • one should be understood as “at least one” or “one or more”, that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the element may be multiple, and the term “one” should not be understood as a limitation on the quantity.
  • the terms “installed”, “connected”, and “connected” should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements.
  • installed should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements.
  • FIG. 1 to FIG. 5 the specific structure of a spinal cord clamp 100 for two-photon recording according to a preferred embodiment of the present application is illustrated.
  • the spinal cord clamp 100 includes a base 10 and a spinal cord clamping module 20 installed on the base 10, the spinal cord clamping module 20 includes a clamping member 21 and a mounting assembly 22 for mounting the clamping member 21 on the base 10, the clamping member 21 includes a first bracket 211 installed on the base 10 and a second bracket 212 slidably disposed on the first bracket 211, the first bracket 211 and the second bracket both have clamping arms extending toward the base 10, and a spinal cord clamping space 213 is formed between the clamping arms of the first bracket 211 and the second bracket 212.
  • the second bracket 212 can slide on the first bracket 211, the distance between the clamping arms of the two brackets can be adjusted, thereby enabling adjustment of the clamping range and clamping force, which is beneficial to improving the flexibility of use and scope of application of the spinal cord clamp 100.
  • first bracket 211 and the second bracket 212 are The brackets 212 can be slidably connected by a screw and a guide rail.
  • the second bracket 212 can slide on the inner guide rail of the first bracket 211 by rotating the screw, which facilitates the adjustment of the clamping force.
  • the present application does not limit the specific method of achieving the sliding connection between the first bracket 211 and the second bracket 212.
  • first bracket 211 and the second bracket 212 are both L-shaped brackets, and the first bracket 211 and the second bracket 212 are connected by a structure in which screw holes and screws match.
  • the two can be locked by screws.
  • the positions of the first bracket 211 and the second bracket 212 can be adjusted again by loosening the screws, thereby facilitating the use of the spinal cord clamp 100.
  • the first bracket 211 includes a connecting portion 2111 for connecting to the base 10, two support rods 2112 extending from the connecting portion 2111 at intervals, two first clamping arms 2113 extending vertically from the corresponding support rods 2112, and a limiting beam 2114 transversely connecting the two support rods 2112.
  • the second bracket 212 includes a base 2121 , two second clamping arms 2122 vertically extending from the base 2121 , and a protrusion 2123 protruding from the back of the base 2121 , and the protrusion 2123 is slidably disposed between the two support rods 2112 .
  • the space from the first clamping arm 2113 of the first bracket 211 to the base 10 is the sliding space where the second bracket 212 can slide between the two support rods 2112 of the first bracket 211.
  • the second clamping arm 2122 of the second bracket 212 moves away from or close to the first clamping arm 2113, thereby adjusting the spinal cord clamping space 213, thereby adjusting the spinal cord clamping range and strength.
  • the second bracket 212 further includes a first screw hole 2124 penetrating the base 2121 and the protrusion 2123, and is used to form a connection with the first bracket 211 through a first screw 215. It can be understood that after the second bracket 212 is slid to the target position, the first bracket 211 and the second bracket 212 can be locked by tightening the first screw 215.
  • the first screw hole 2124 is a 2.5 mm screw hole.
  • the first screw hole 2124 may also be of other sizes and may be set according to actual needs, which is not limited by the present application.
  • a rectangular opening 2115 is formed between the two support rods 2112 of the first bracket 211, and the raised portion 2123 of the second bracket 212 is a rectangular block structure.
  • the present application limits the range of motion of the second bracket 212 through the rectangular opening 2115 between the two support rods 2112, and prevents the first bracket 211 from being deformed at the connection, having a loose connection, or having loose teeth after being used multiple times.
  • the present application also connects the two support rods 2112 through the limiting crossbeam 2114, which can ensure the stability and strength of the rectangular opening 2115 between the two support rods 2112, ensure the structural stability of the entire spinal cord clamping module 20, and is beneficial to avoid causing field of view shaking and thus improve imaging clarity when the spinal cord clamp 100 is used for two-photon recording.
  • first clamping arm 2113 and the second clamping arm 2122 are both provided with a tooth structure 214 .
  • toothed structure 214 is provided on the first clamping arm 2113 and the second clamping arm 2122 in the present application, so as to increase the clamping friction force and ensure the firmness of the spinal cord clamp 100 and the spine.
  • the base 10 includes a bottom plate 11 and a mounting block 12 disposed on the bottom plate 11, and a side of the mounting block 12 is provided with a connection portion 2111 for mounting the first bracket 211.
  • this specific example of the present application shows a base 10 adapted to a specific two-photon microscope model, and the base 10 can be adapted to be designed according to different types of microscope translation stages, and the present application does not impose any limitation on this.
  • the size of the groove 121 is 0.5*8*10 mm.
  • the bottom plate 11 is provided with four third screw holes 111 for connecting with the two-photon microscope translation stage through screws.
  • the second screw hole 122 is a 2.5 mm screw hole
  • the third screw hole 111 is a 6 mm screw hole.
  • the second screw hole 122, the third screw hole 111 and the groove 121 may also adopt other sizes and can be set according to actual needs, which is not limited by the present application.
  • the spinal cord clamp 100 can also be equipped with a heating device on the base 10 to keep the body temperature of the mouse constant during the recording process under anesthesia.
  • the first bracket 211 and the second bracket 212 of the present application are connected by screws, and the second bracket 212 can slide inside the first bracket 211, and the clamping arms of the first bracket 211 and the second bracket 212 are provided with relative tooth structures 214 that can be clamped on both sides of the mouse spine. By sliding the second bracket 212, the clamping range and clamping tightness can be adjusted.
  • the base 10 and the cover plate 221 are connected by screws, and the side of the base 10 is provided with the groove 121 for By clamping the first bracket 211 and tightening the second screw 222, the second bracket 212 and the base 10 can be firmly combined to ensure the stability of the overall connection.
  • 6mm screw holes are set at the four corners of the base 10, which can be tightly combined with the two-photon microscope translation stage, which is conducive to ensuring the stability of the connection between the spinal cord clamp 100 and the two-photon microscope translation stage, reducing vibration, and thus ensuring good imaging quality.
  • the spinal cord clamp 100 of the present application uses the rectangular opening 2115 in the first bracket 211 to limit the range of motion of the second bracket 212, and prevents the first bracket 211 from deforming the connection, loose connection, loose teeth, etc. after repeated use. Long-term use can also firmly restrain the mouse in a lateral position on the two-photon microscope translation stage to prevent the mouse's heartbeat, breathing and activity from affecting the imaging quality.
  • the present application provides a spinal cord clamp 100 for two-photon recording with an adjustable clamping range, high clamping strength, and high structural stability, which can solve the technical problem that the existing spinal cord fixation devices have poor stability and durability, resulting in low spinal cord fixation firmness, which easily causes field of view shaking and affects the imaging quality.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
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Abstract

一种用于双光子记录的脊髓夹持器，包括基座（10）和安装于基座（10）的脊髓夹持模块（20），脊髓夹持模块（20）包括夹持件（21）和用于将夹持件（21）安装在基座上的安装组件（22），夹持件（21）包括安装在基座（10）上的第一支架（211）和可滑动地设置于第一支架（211）的第二支架（212），第一支架（211）和二支架（212）均具有向基座（10）延伸的夹持臂，第一支架（211）和第二支架（212）的夹持臂之间形成脊髓夹持空间（213）。脊髓夹持器（100）的夹持范围可调，夹持强度高，并具有较高的结构稳定性和持久性，能够避免引起双光子显微镜的视野晃动，确保良好的成像质量。

Description

一种用于双光子记录的脊髓夹持器 技术领域
本申请涉及动物实验设备技术领域，特别是涉及一种用于双光子记录的脊髓夹持器。
背景技术
背根神经节是人和脊椎动物感知外周刺激的重要周围神经组织，也是将外周感觉编码传递到脊髓的重要驿站。研究感知觉如痛、痒、触、冷和热等感知觉的神经编码机制就需要研究在不同外周条件刺激情况下背根神经节神经元的反应差异。
目前双光子显微镜用于记录背根神经元活动解析外周感觉的编码机制属于神经科学的前沿领域，但目前用于双光子记录的脊髓固定设备发展还不完善，由于现有的脊髓固定设备的稳定性和持久性较差，会导致脊髓固定牢固度不高，因此容易引起视野晃动从而影响成像质量。
发明内容
本申请的一目的是，提供一种夹持范围可调、夹持强度高、结构稳定性高的用于双光子记录的脊髓夹持器，以解决现有的脊髓固定设备的稳定性和持久性较差，会导致脊髓固定牢固度不高，从而容易引起视野晃动从而影响成像质量的技术问题。
本申请提供了一种用于双光子记录的脊髓夹持器，包括基座和安装于所述 基座的脊髓夹持模块，所述脊髓夹持模块包括夹持件和用于将所述夹持件安装在所述基座上的安装组件，所述夹持件包括安装在所述基座上的第一支架和可滑动地设置于所述第一支架的第二支架，所述第一支架和所述二支架均具有向所述基座延伸的夹持臂，所述第一支架和所述第二支架的夹持臂之间形成脊髓夹持空间。
在本申请的一实施例中，所述第一支架和所述第二支架均为L形支架，所述第一支架和所述第二支架通过螺丝孔和螺钉相匹配的结构形成连接。
在本申请的一实施例中，所述第一支架包括用于与所述基座连接的连接部、间隔延伸自所述连接部的两个支杆、分别垂直延伸自对应的支杆的两个第一夹持臂以及横向连接两个支杆的限位横梁。
在本申请的一实施例中，所述第二支架包括基部、垂直延伸自所述基部的两个第二夹持臂以及凸出延伸自所述基部的背部的凸起部，所述凸起部可滑动地设置在两个支杆之间。
在本申请的一实施例中，所述第一夹持臂和所述第二夹持臂上均设置有齿状结构。
在本申请的一实施例中，所述第二支架还包括贯穿所述基部和所述凸起部的第一螺丝孔，用于通过第一螺钉与所述第一支架形成连接。
在本申请的一实施例中，所述支杆之间形成矩形开口，用于限制所述第二支架的活动范围。
在本申请的一实施例中，所述第一螺丝孔为2.5mm螺丝孔。
在本申请的一实施例中，所述基座包括底板和设置在所述底板上的安装块，所述安装块的侧面设置有用于安装所述第一支架的所述连接部的凹槽和分别设置于所述凹槽两侧的两个第二螺丝孔，所述安装组件包括用于将所述第一 支架固定在所述安装块上的盖板和用于与所述第二螺丝孔连接以将所述盖板固定在所述安装块上的两个第二螺钉。
在本申请的一实施例中，所述底板设置有四个第三螺丝孔，用于通过螺钉和双光子显微镜位移台形成连接。
在本申请的一实施例中，所述基座设置有加热装置。
在本申请的一实施例中，所述第二螺丝孔为2.5mm螺丝孔，所述第三螺丝孔为6mm螺丝孔。
本申请具有以下有益效果：
(1)本申请的脊髓夹持器通过两个支架之间的可滑动连接，能够实现夹持范围和夹持力度的调节，有利于提高使用灵活性和适用范围；
(2)本申请在第一支架的两个支杆上设置有限位横梁，能够保证第一支架开口端的稳定性和强度，确保夹持稳定性；
(3)本申请在两个夹持臂上设置齿状结构，能够增大夹持摩擦力，确保脊髓夹持器与脊柱夹持的牢固性；
(4)本申请在基座上设置有多个螺丝孔，能够便于脊髓夹持器与双光子显微镜位移台形成牢固连接。
通过对随后的描述和附图的理解，本申请进一步的目的和优势将得以充分体现。
附图说明
图1为本申请的一优选实施例的用于双光子记录的脊髓夹持器的立体结构示意图。
图2为图1所示的用于双光子记录的脊髓夹持器在另一视角下的立体结构示意图。
图3为图1所示的用于双光子记录的脊髓夹持器的爆炸图。
图4为图1所示的用于双光子记录的脊髓夹持器的第一支架的立体结构示意图。
图5为图1所示的用于双光子记录的脊髓夹持器的第二支架的立体结构示意图。
附图标号说明：脊髓夹持器100；基座10；底板11；第三螺丝孔111；安装块12；凹槽121；第二螺丝孔122；脊髓夹持模块20；夹持件21；第一支架211；连接部2111；支杆2112；第一夹持臂2113；限位横梁2114；矩形开口2115；第二支架212；基部2121；第二夹持臂2122；凸起部2123；第一螺丝孔2124；脊髓夹持空间213；齿状结构214；第一螺钉215；安装组件22；盖板221；第二螺钉222。
具体实施方式
以下描述用于揭露本申请以使本领域技术人员能够实现本申请。以下描述中的优选实施例只作为举例，本领域技术人员可以想到其他显而易见的变型。在以下描述中界定的本申请的基本原理可以应用于其他实施方案、形变方案、改进方案、等同方案以及没有背离本申请的精神和范围的其他技术方案。
本领域技术人员应理解的是，在本申请的揭露中，术语“竖向”、“横向”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”“内”、“外”等指示的方位或位置关系是基于附图所示的方位或位置关系，其仅是为了便于描述本申请和简化描述，而不是指示 或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此上述术语不能理解为对本申请的限制。
可以理解的是，术语“一”应理解为“至少一”或“一个或多个”，即在一个实施例中，一个元件的数量可以为一个，而在另外的实施例中，该元件的数量可以为多个，术语“一”不能理解为对数量的限制。
在本申请的描述中，需要说明的是，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接或可以相互通讯；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本申请中的具体含义。
如图1至图5所示，根据本申请的一优选实施例的一种用于双光子记录的脊髓夹持器100的具体结构被阐明。
如图1至图3所示，所述脊髓夹持器100包括基座10和安装于所述基座10的脊髓夹持模块20，所述脊髓夹持模块20包括夹持件21和用于将所述夹持件21安装在所述基座10上的安装组件22，所述夹持件21包括安装在所述基座10上的第一支架211和可滑动地设置于所述第一支架211的第二支架212，所述第一支架211和所述二支架均具有向所述基座10延伸的夹持臂，所述第一支架211和所述第二支架212的夹持臂之间形成脊髓夹持空间213。
可以理解的是，由于所述第二支架212能够在所述第一支架211上滑动，使得两个支架之间的夹持臂的距离能够被调节，从而能够实现夹持范围和夹持力度的调节，有利于提高所述脊髓夹持器100的使用灵活性和适用范围。
应该理解的是，在本申请的一些实施例中，所述第一支架211和所述第二 支架212之间可以通过丝杆加导轨的连接方式实现滑动连接，通过转动丝杆使所述第二支架212在所述第一支架211内侧导轨上滑动，方便夹持力度的调节，本申请对所述第一支架211和所述第二支架212实现滑动连接的具体方式不作限制。
特别地，在本申请的这一具体实施例中，所述第一支架211和所述第二支架212均为L形支架，所述第一支架211和所述第二支架212通过螺丝孔和螺钉相匹配的结构形成连接。
可以理解的是，通过将所述第二支架212在所述第一支架211上滑动到目标位置时，通过螺钉可以将两者锁定，在针对不同夹持需求时，可以通过拧松螺钉再次调节所述第一支架211和所述第二支架212的位置，以此方便所述脊髓夹持器100的使用。
具体地，如图4所示，所述第一支架211包括用于与所述基座10连接的连接部2111、间隔延伸自所述连接部2111的两个支杆2112、分别垂直延伸自对应的支杆2112的两个第一夹持臂2113以及横向连接两个支杆2112的限位横梁2114。
如图5所示，所述第二支架212包括基部2121、垂直延伸自所述基部2121的两个第二夹持臂2122以及凸出延伸自所述基部2121的背部的凸起部2123，所述凸起部2123可滑动地设置在两个支杆2112之间。
可以理解的是，所述第一支架211的所述第一夹持臂2113至所述基座10的空间即为所述第二支架212可以在所述第一支架211两个支杆2112之间的滑动空间，在所述二支架滑动时，所述第二支架212的所述第二夹持臂2122远离或者靠近所述第一夹持臂2113，从而实现对所述脊髓夹持空间213的调节，以此实现对脊髓夹持范围与力度的调节。
进一步地，所述第二支架212还包括贯穿所述基部2121和所述凸起部2123的第一螺丝孔2124，用于通过第一螺钉215与所述第一支架211形成连接。可以理解的是，在将所述第二支架212滑动至目标位置后，通过拧紧所述第一螺钉215，即可以将所述第一支架211和所述第二支架212锁定。
值得一提的是，在本申请的这一具体实施例中，所述第一螺丝孔2124采用2.5mm螺丝孔。在本申请的其他的一些实施例中，所述第一螺丝孔2124也可以采用其他尺寸，可根据实际需要进行设置，本申请对此不作限制。
还可以理解的是，所述第一支架211的两个支杆2112之间形成矩形开口2115，所述第二支架212的所述凸起部2123为矩形块状结构，本申请通过两个支杆2112之间的矩形开口2115来限制所述第二支架212的活动范围，并防止所述第一支架211多次使用后出现的连接处变形、结合不牢固、对齿不严密等问题。
特别地，本申请还通过所述限位横梁2114来连接两个所述支杆2112，能够保证两个所述支杆2112之间的矩形开口2115端的稳定性和强度，确保整个脊髓夹持模块20的结构稳定性，有利于在所述脊髓夹持器100应用于双光子记录时，能够避免引起视野晃动从而改善成像清晰度。
进一步地，所述第一夹持臂2113和所述第二夹持臂2122上均设置有齿状结构214。
可以理解的是，本申请在所述第一夹持臂2113和所述第二夹持臂2122上设置齿状结构214，能够增大夹持摩擦力，确保脊髓夹持器100与脊柱夹持的牢固性。
进一步地，所述基座10包括底板11和设置在所述底板11上的安装块12，所述安装块12的侧面设置有用于安装所述第一支架211的所述连接部2111的 凹槽121和分别设置于所述凹槽121两侧的两个第二螺丝孔122，所述安装组件22包括用于将所述第一支架211固定在所述安装块12上的盖板221和用于与所述第二螺丝孔122连接以将所述盖板221固定在所述安装块12上的两个第二螺钉222。
应该理解的是，本申请的这一具体实例展示的是与特定双光子显微镜型号适配的基座10，可根据不同显微镜位移台类型设计适配基座10，本申请对此不作限制。
值得一提的是，在本申请的这一具体实施例中，所述凹槽121的尺寸为0.5*8*10mm。
特别地，所述底板11设置有四个第三螺丝孔111，用于通过螺钉和双光子显微镜位移台形成连接。
值得一提的是，在本申请的这一具体实施例中，所述第二螺丝孔122为2.5mm螺丝孔，所述第三螺丝孔111为6mm螺丝孔。
在本申请的其他的一些实施例中，所述第二螺丝孔122、所述第三螺丝孔111以及所述凹槽121也可以采用其他尺寸，可根据实际需要进行设置，本申请对此不作限制。
还值得一提的是，所述脊髓夹持器100还可以在所述基座10增设加热装置，使小鼠在麻醉状态下的记录过程中保持体温恒定。
本申请的所述第一支架211和所述第二支架212之间通过螺丝结合，所述第二支架212可以在所述第一支架211内侧滑动，且所述第一支架211和所述第二支架212的夹持臂上设置有相对的齿状结构214可以卡在小鼠脊柱两侧，通过滑动所述第二支架212即可调节夹持范围和夹持松紧度。所述的基座10和所述盖板221通过螺丝连接，所述基座10的侧面设置有所述凹槽121用于 卡住所述第一支架211，通过拧紧所述第二螺钉222则可以将所述第二支架212和所述基座10牢固结合，确保整体连接的稳定性。另外，所述基座10四角处设置有6mm螺丝孔，可以与双光子显微镜位移台紧密结合，有利于确保所述脊髓夹持器100与双光子显微镜位移台连接的稳定性，减少震动，从而确保良好的成像质量。
本申请的所述脊髓夹持器100利用所述第一支架211内的矩形开口2115限制了所述第二支架212的活动范围，并防止所述第一支架211多次使用后出现的连接处变形、结合不牢固、对齿不严密等问题。长期使用也能够将小鼠以侧卧位牢固地束缚在双光子显微镜位移台上，避免小鼠的心跳、呼吸和活动对成像质量影响。
总的来讲，本申请提供了一种夹持范围可调、夹持强度高、结构稳定性高的用于双光子记录的脊髓夹持器100，能够解决现有的脊髓固定设备的稳定性和持久性较差，会导致脊髓固定牢固度不高，从而容易引起视野晃动从而影响成像质量的技术问题。
以上实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上实施例仅表达了本申请的优选的实施方式，其描述较为具体和详细，但并不能因此而理解为对本申请专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本申请构思的前提下，还可以做出若干变形和改进，这些都属于本申请的保护范围。因此，本申请专利的保护范围应以所附权利要求为准。

Claims (10)

  1. 一种用于双光子记录的脊髓夹持器，其特征在于，包括基座和安装于所述基座的脊髓夹持模块，所述脊髓夹持模块包括夹持件和用于将所述夹持件安装在所述基座上的安装组件，所述夹持件包括安装在所述基座上的第一支架和可滑动地设置于所述第一支架的第二支架，所述第一支架和所述二支架均具有向所述基座延伸的夹持臂，所述第一支架和所述第二支架的夹持臂之间形成脊髓夹持空间。
  2. 根据权利要求1所述的用于双光子记录的脊髓夹持器，其特征在于，所述第一支架和所述第二支架均为L形支架，所述第一支架和所述第二支架通过螺丝孔和螺钉相匹配的结构形成连接。
  3. 根据权利要求1所述的用于双光子记录的脊髓夹持器，其特征在于，所述第一支架包括用于与所述基座连接的连接部、间隔延伸自所述连接部的两个支杆、分别垂直延伸自对应的支杆的两个第一夹持臂以及横向连接两个支杆的限位横梁。
  4. 根据权利要求3所述的用于双光子记录的脊髓夹持器，其特征在于，所述第二支架包括基部、垂直延伸自所述基部的两个第二夹持臂以及凸出延伸自所述基部的背部的凸起部，所述凸起部可滑动地设置在两个支杆之间。
  5. 根据权利要求4所述的用于双光子记录的脊髓夹持器，其特征在于，所述支杆之间形成矩形开口，用于限制所述第二支架的活动范围。
  6. 根据权利要求4所述的用于双光子记录的脊髓夹持器，其特征在于，所述第一夹持臂和所述第二夹持臂上均设置有齿状结构。
  7. 根据权利要求4所述的用于双光子记录的脊髓夹持器，其特征在于，所述第二支架还包括贯穿所述基部和所述凸起部的第一螺丝孔，用于通过第一 螺钉与所述第一支架形成连接。
  8. 根据权利要求3至7中任一项所述的用于双光子记录的脊髓夹持器，其特征在于，所述基座包括底板和设置在所述底板上的安装块，所述安装块的侧面设置有用于安装所述第一支架的所述连接部的凹槽和分别设置于所述凹槽两侧的两个第二螺丝孔，所述安装组件包括用于将所述第一支架固定在所述安装块上的盖板和用于与所述第二螺丝孔连接以将所述盖板固定在所述安装块上的两个第二螺钉。
  9. 根据权利要求8所述的用于双光子记录的脊髓夹持器，其特征在于，所述底板设置有四个第三螺丝孔，用于通过螺钉和双光子显微镜位移台形成连接。
  10. 根据权利要求9所述的用于双光子记录的脊髓夹持器，其特征在于，所述基座设置有加热装置。
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