WO2024182295A3 - Targeted spatial sequencing - Google Patents

Targeted spatial sequencing Download PDF

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Publication number
WO2024182295A3
WO2024182295A3 PCT/US2024/017299 US2024017299W WO2024182295A3 WO 2024182295 A3 WO2024182295 A3 WO 2024182295A3 US 2024017299 W US2024017299 W US 2024017299W WO 2024182295 A3 WO2024182295 A3 WO 2024182295A3
Authority
WO
WIPO (PCT)
Prior art keywords
targeted spatial
spatial sequencing
sequencing
targeted
amplifying
Prior art date
Application number
PCT/US2024/017299
Other languages
French (fr)
Other versions
WO2024182295A2 (en
Inventor
Martin Maria FABANI
Michael Lawson
Original Assignee
Singular Genomics Systems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Singular Genomics Systems, Inc. filed Critical Singular Genomics Systems, Inc.
Publication of WO2024182295A2 publication Critical patent/WO2024182295A2/en
Publication of WO2024182295A3 publication Critical patent/WO2024182295A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed herein, inter alia, are oligonucleotide probes, methods, and kits useful for amplifying and detecting target nucleic acids in cells and tissues.
PCT/US2024/017299 2023-02-27 2024-02-26 Targeted spatial sequencing WO2024182295A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202363487200P 2023-02-27 2023-02-27
US63/487,200 2023-02-27
US202463621418P 2024-01-16 2024-01-16
US63/621,418 2024-01-16

Publications (2)

Publication Number Publication Date
WO2024182295A2 WO2024182295A2 (en) 2024-09-06
WO2024182295A3 true WO2024182295A3 (en) 2024-10-24

Family

ID=92590413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/017299 WO2024182295A2 (en) 2023-02-27 2024-02-26 Targeted spatial sequencing

Country Status (1)

Country Link
WO (1) WO2024182295A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060166245A1 (en) * 2003-07-17 2006-07-27 Potter S S Rolling circle amplification of micro-RNA samples
US20230031996A1 (en) * 2021-07-30 2023-02-02 10X Genomics, Inc. Circularizable probes for in situ analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060166245A1 (en) * 2003-07-17 2006-07-27 Potter S S Rolling circle amplification of micro-RNA samples
US20230031996A1 (en) * 2021-07-30 2023-02-02 10X Genomics, Inc. Circularizable probes for in situ analysis

Also Published As

Publication number Publication date
WO2024182295A2 (en) 2024-09-06

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