WO2024092878A1 - 一种自动化核酸试剂成品制作装置 - Google Patents

一种自动化核酸试剂成品制作装置 Download PDF

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Publication number
WO2024092878A1
WO2024092878A1 PCT/CN2022/131934 CN2022131934W WO2024092878A1 WO 2024092878 A1 WO2024092878 A1 WO 2024092878A1 CN 2022131934 W CN2022131934 W CN 2022131934W WO 2024092878 A1 WO2024092878 A1 WO 2024092878A1
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Prior art keywords
liquid
nucleic acid
module
integrated module
finished product
Prior art date
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Ceased
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PCT/CN2022/131934
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English (en)
French (fr)
Inventor
胡松
曾钊宇
麦梅珍
李新民
李政江
冯进旺
彭幼鹏
李明坚
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Hanjia Xinghua Technology Foshan Co Ltd
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Hanjia Xinghua Technology Foshan Co Ltd
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Priority claimed from CN202211357126.5A external-priority patent/CN115518705A/zh
Priority claimed from CN202222896337.8U external-priority patent/CN221907169U/zh
Application filed by Hanjia Xinghua Technology Foshan Co Ltd filed Critical Hanjia Xinghua Technology Foshan Co Ltd
Publication of WO2024092878A1 publication Critical patent/WO2024092878A1/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes

Definitions

  • the utility model relates to the technical field of molecular diagnosis and biomedical equipment, and in particular is an intelligent automatic nucleic acid reagent finished product production device.
  • Nucleic acid testing commonly known as molecular testing, and real-time fluorescence quantitative polymerase chain reaction technology are emerging industrial fields that have rapidly emerged in the past decade along with genomic technology.
  • the mixing effect of the reagents will also affect the final test results. Therefore, the reagent mixing device plays an important role in the entire reaction process.
  • Conventional mixing methods include stirring, oscillation and ultrasound. Stirring and ultrasound affect the reliability and accuracy of the test results. Generally, oscillation mixing is used to mix the reagents, but the compatibility of adding a temperature control device is poor.
  • the utility model aims to provide an intelligent automatic nucleic acid reagent finished product production device, which achieves the purpose of one-step and efficient production of nucleic acid reagent finished products while ensuring the performance of nucleic acid detection reagents.
  • An automated nucleic acid reagent finished product production device comprises a housing, wherein two or more work area platforms, a cover closing module and a control system for controlling operation are installed in the housing, and a transmission integrated module for conveying a carrier is arranged across the work area platforms and the cover closing module;
  • the work area platform includes a lifting and moving module that can be lifted and lowered, a liquid adding integrated module for adding liquid, and a liquid control integrated module for controlling the amount of liquid added and the temperature;
  • the liquid adding integrated module is driven to rise or fall by the lifting module to add liquid.
  • the liquid adding integrated module is connected to the hydraulic control integrated module.
  • the control system controls the transmission integrated module to transport the carrier to the working area platform to add liquid and transport it to the capping module to complete the capping and packaging after the liquid adding is completed.
  • the liquid control integrated module is provided with liquid flow pipelines for heat preservation and mixing.
  • the container is provided with two openings, the two openings of the container are respectively connected to the liquid adding pipe and the liquid flow pipeline; the liquid adding pipeline is connected and installed with a precision pump, and the liquid flow inlet of the liquid adding integrated module is connected to the liquid flow pipeline.
  • the two openings of the container are connected to the liquid adding pipe and the liquid flow pipeline respectively through a straight joint; the outer periphery of the liquid flow pipeline is wrapped with insulation material, and the liquid flow pipeline and the liquid adding channel are both provided with liquid level alarms; a barrel is installed at the bottom of the shell, and the container is placed in the barrel.
  • the transmission integrated module includes a sensor, a motor, a conveyor belt, and a slide rail.
  • the slide rail crosses the middle of the shell, the conveyor belt is laid on the slide rail, the carrier equipped with the sensor is installed on the conveyor belt, and the conveyor belt is driven by the motor to make the carrier on the conveyor belt move along the slide rail.
  • the shell includes a higher boss in the middle and lower platforms at both ends, the working area platform and the lid closing module are arranged in sequence in the boss, the slide rail is arranged horizontally in a straight line, the two ends of the conveyor belt are laid on the top of the platform, and the middle part crosses the boss and passes through the working area platform and the lid closing module to perform liquid adding and lid closing operations.
  • the liquid adding integrated module includes a liquid adding needle and a liquid outlet port at the bottom of the liquid adding needle for discharging liquid
  • the lifting and moving module includes a needle holder mechanism, a mounting frame, a lifting mechanism and a motor; the mounting frame is provided with a lifting mechanism, the needle holder mechanism can be lifted and installed in the lifting mechanism, the liquid adding needle is installed on the needle holder mechanism, the top of the liquid adding needle is connected to the liquid adding tube, and the lifting mechanism is driven by a motor to make the liquid adding needle on the needle holder mechanism rise and fall for adding liquid.
  • the closing module includes a closing key plate, a mounting frame and a lifting mechanism, the closing key plate can be slidably lifted and lowered on the mounting frame through the lifting mechanism, and a protruding key strip for closing is provided at the bottom of the closing key plate.
  • the mounting frame includes a mounting plate, guide rods vertically fixed on the mounting plate and a limit plate fixed between the guide rods, the needle holder mechanism is fixed on the limit plate, and the motor drives the limit plate to slide and rise and fall along the guide rods, and the mounting frame is equipped with a sensor for sensing the reaction tube on the carrier and a calibration groove for liquid level calibration and measuring the liquid level height of the reaction tube.
  • control system includes sensors, signal transmission modules, signal processing modules and displays, the working area platform, the device and the carrier are all equipped with sensors, the position of the reaction tube on the carrier is sensed by the sensors, the signal transmission module transmits the signal to the signal processing module for analysis and processing, and then controls the working area platform to add liquid and release, the closing module to close and release, and the transmission integrated module to transport the carrier to complete the liquid adding and closing processes according to a preset program.
  • cabinet doors are movably connected to the left and right sides of the front surface of the shell through hinges, door buckles are recessed on the surfaces of the cabinet doors, and universal wheels are installed at the bottom of the shell.
  • a further measure taken is that the bottom surface of the protruding key strip is horizontal, and one side in the same direction is arc-shaped.
  • the precision pump can be one of a micro gear pump, a picoliter pump, a syringe pump, a plunger pump, a peristaltic pump, a piezoelectric valve, and a solenoid valve.
  • control system adopts PLC, which can be divided into closed-loop control system and program control system, which can give full play to the function of PLC and meet the control requirements of the controlled object to the greatest extent.
  • a calibration tank for liquid level calibration and measuring the liquid level height of the reaction tube is installed below the liquid adding integrated module, and a sensor for sensing the reaction tube on the carrier is also installed below the liquid adding integrated module.
  • the calibration tank is connected to the container through a tank pipe, the tank pipe is connected to a precision pump, the tank pipe is placed in the container through a straight-through joint, the calibration tank receives the test liquid flowing out of the liquid adding integrated module by motor-driven displacement and measures and calibrates through a precision pump.
  • the calibration tank and its related components are used to detect the liquid of the dosing integrated module before refilling to ensure the accuracy and precision of the dosing.
  • the calibration tank also has a liquid collection and recovery function.
  • the sensor detects the status of all dosing needles and plays a role in calibrating the liquid discharge status of the dosing needles. That is, automatic calibration is performed when the machine is turned on.
  • the equipment starts initialization.
  • the calibration tank is driven by the motor to move under the dosing needle.
  • the dosing needle injects liquid into the calibration tank.
  • the precision pump connected to the calibration tank starts to pump the liquid into the container.
  • the sensor detects the status of all dosing needles. If the status of the dosing needle meets the requirements, the calibration tank returns to its original position and enters the working state.
  • control system includes sensors, signal transmission modules, signal processing modules and displays, the working area platform, the device and the carrier are all equipped with sensors, the position of the reaction tube on the carrier is sensed by the sensors, the signal transmission module transmits the signal to the signal processing module for analysis and processing, and then controls the working area platform to add liquid and release, the closing module to close and release, and the transmission integrated module to transport the carrier to complete the liquid adding and closing processes according to a preset program.
  • cabinet doors are movably connected to the left and right sides of the front surface of the shell through hinges, door buckles are recessed on the surface of the cabinet doors, and universal wheels are installed at the bottom of the shell;
  • the bottom surface of the protruding key strip is horizontal, and the same side is arc-shaped;
  • the precision pump can adopt one of a micro gear pump, a picoliter pump, a syringe pump, a plunger pump, a peristaltic pump, a piezoelectric valve, and a solenoid valve;
  • the control system adopts a PLC system.
  • the number of the workspace platforms is three, including a first workspace platform, a second workspace platform and a third workspace platform:
  • the main indicators and parameters of the nucleic acid reagent finished product production device are as follows: working power supply: AC 220V, input power: about 2 KW, ambient temperature: 21-25°C (no freezing), relative humidity: ⁇ 65% (no condensation), temperature range of the first working area platform: 1-5°C (no freezing), temperature range of the second working area platform: 10-35°C, temperature range of the third working area platform: -20°C-5°C, liquid addition range: 1-50 ⁇ L, liquid addition accuracy: 1-5 ⁇ L ⁇ 2%, 6-50 ⁇ L ⁇ 1%, working mode: S2, production efficiency; 4800-96000PCS/H, bottle sealing model: PCR reaction tube, positioning accuracy: 0.05MM.
  • a conveying integrated module for conveying a carrier is arranged across the working area platform and the cover closing module, so that the reaction tube can be placed on the carrier, and the control system controls the conveying integrated module to convey the reaction tube on the carrier to the working area platform for adding various raw material liquids, and after the liquid addition is completed, it continues to be conveyed to the cover closing module to complete the cover closing and packaging, thereby automatically completing the configuration of nucleic acid detection reagent materials and directly producing the finished product, realizing one-step production of nucleic acid reagent finished products, without the participation of too many instruments and equipment and tedious and rigorous preparation operations; at the same time, the production is completed in the shell, avoiding the risks of exposure and contamination, and the production is protected from light, which better guarantees the performance of the nucleic acid detection reagent.
  • the technical solution is easy to operate, automated, and efficient, and can also achieve large-scale (million-
  • FIG1 is a perspective view of the external structure of the utility model
  • FIG2 is a bottom view of the structure of the utility model
  • FIG3 is a top view of the internal structure of the utility model
  • FIG4 is a front view of the internal structure of the utility model
  • FIG5 is a front view of the working area platform structure of the utility model
  • FIG6 is a front view of the cover closing module structure of the utility model
  • FIG. 7 is a flowchart of a nucleic acid reagent finished product production device according to an embodiment of the present invention.
  • An automated nucleic acid reagent finished product production device includes a shell 1, in which are installed more than two working area platforms 2, a lid closing module 3 and a control system for controlling the operation of the operation.
  • a conveying integrated module 4 for conveying a carrier 402 is arranged across the working area platform 2 and the lid closing module 3;
  • the working area platform 2 includes a liftable lifting and moving module 5, a liquid adding integrated module 6 for adding liquid and a liquid control integrated module 7 for controlling the amount of liquid added and the temperature;
  • the liquid adding integrated module 6 is driven to rise or fall by the lifting module to add liquid, and the liquid adding integrated module 6 is connected to the liquid control integrated module 7, and the carrier 402 is conveyed to the working area platform 2 through the conveying integrated module 4 to add liquid and is conveyed to the lid closing module 3 to complete the lid closing and packaging after the liquid adding is completed.
  • a conveying integrated module 4 for conveying a carrier 402 is arranged across the working area platform 2 and the lid closing module 3, so that the reaction tube can be placed on the carrier 402, and the control system controls the conveying integrated module 4 to convey the reaction tube on the carrier 402 to the working area platform 2 for adding various raw material liquids, and after the liquid addition is completed, it continues to be conveyed to the lid closing module 3 to complete the lid closing and packaging, thereby automatically completing the configuration of nucleic acid detection reagent materials and directly making it into a finished product, realizing the one-step production of nucleic acid reagent finished products without the involvement of too many instruments and equipment and cumbersome and rigorous preparation, and completing the production in the shell 1 at the same time, avoiding the risk of exposure and contamination, and better ensuring the performance of the nucleic acid detection reagent.
  • the technical solution is easy to operate, automated, and efficient, and
  • the liquid control integrated module 7 is provided with liquid flow pipelines for heat preservation and mixing. Meanwhile, the liquid flow pipelines utilize liquid flow and circulation flow to achieve the purpose of mixing the reagents and raw materials.
  • liquid flow pipeline By laying a liquid flow pipeline for insulation in the liquid control integrated module 7, this ingenious design allows the liquid flowing in the liquid adding tube 702 to also be insulated, thereby ensuring the temperature of the liquid flow during the liquid adding process.
  • the form of the liquid flow pipeline avoids the introduction of complex heating control devices and has high compatibility. Different working area platforms 2 can be completely controlled in different temperature ranges to ensure the preparation process and performance of nucleic acid reagents.
  • the liquid control integrated module 7 includes a precision pump 701, a liquid adding pipe 702 and a temperature control device.
  • the liquid adding pipe 702 is connected to the precision pump 701, so that the amount of liquid added can be accurately controlled.
  • the two ends of the liquid adding pipe 702 are respectively connected to the liquid flow inlet of the liquid adding integrated module 6 and the container 8.
  • the liquid flow pipeline is wrapped and laid close to the liquid adding pipe 702, so that the thermal insulation effect is better and will not affect the temperature of other working areas.
  • the container 8 is equipped with a temperature control device for adjusting the temperature of the liquid in the container 8. The temperature of the liquid can be better adjusted through the container 8, which is convenient for heating or cooling temperature control.
  • the container 8 is provided with two openings, and the two openings of the container 8 are respectively connected to the liquid adding pipe 702 and the liquid flow pipeline; the liquid adding pipe 702 is connected and installed with a precision pump 701, and the liquid flow inlet of the liquid adding integrated module 6 is connected to the liquid flow pipeline.
  • the temperature of the liquid flow in the liquid adding pipe 702 can be better maintained, and at the same time, there is no need to introduce other liquid flows through the liquid flow pipeline, and the thermal insulation effect is better, and the liquid flow can be conveniently replenished when necessary.
  • the two openings of the container 8 are connected to the liquid adding pipe 702 and the liquid flow pipeline respectively through straight joints; the outer periphery of the liquid flow pipeline is wrapped with insulation material to achieve a better insulation effect, and the liquid flow pipeline and the liquid adding channel are both provided with liquid level alarms to remind the liquid flow level; a barrel 9 is installed at the bottom of the shell 1, and the container 8 is placed in the barrel 9.
  • the conveying integrated module 4 includes a sensor, a motor 506, a conveyor belt, and a slide rail 401.
  • the slide rail 401 runs across the middle of the shell 1.
  • the conveyor belt is laid on the slide rail 401.
  • the carrier 402 equipped with a sensor is installed on the conveyor belt.
  • the conveyor belt is driven by the motor 506 so that the carrier 402 on the conveyor belt moves along the slide rail 401, thereby realizing the transportation of the carrier 402 and realizing automatic assembly line operation to prepare the finished product.
  • the shell 1 includes a higher boss 101 in the middle and lower platforms 102 at both ends.
  • the working area platform 2 and the lid closing module 3 are arranged in sequence in the boss 101.
  • the slide rail 401 is horizontally arranged in a straight line.
  • the two ends of the conveyor belt are laid on the top of the platform 102, so as to facilitate the operation of placing the reaction tube on the carrier 402 and taking out the finished reaction tube packaged with lids at the end.
  • the middle part crosses the boss 101 and passes through the working area platform 2 and the lid closing module 3 to perform liquid adding and lid closing operations, which ensures light protection during the intermediate liquid adding process and provides a relatively closed space.
  • the liquid adding integrated module 6 includes a liquid adding needle 601 and a liquid outlet port for discharging liquid at the bottom of the liquid adding needle 601;
  • the lifting and moving module 5 includes a needle holder mechanism, a mounting frame 501, a lifting mechanism 505 and a motor 506;
  • the mounting frame 501 is provided with a lifting mechanism 505, the needle holder mechanism can be lifted and installed in the lifting mechanism 505, the liquid adding needle 601 is installed on the needle holder mechanism, the top of the liquid adding needle 601 is connected to the liquid adding tube 702, and the lifting mechanism 505 is driven by the motor 506 to make the liquid adding needle 601 on the needle holder mechanism rise and fall for adding liquid, so that liquid addition can be performed flexibly and automatically.
  • the lid closing module 3 includes a lid closing key plate 301, a mounting frame 501 and a lifting mechanism 505.
  • the lid closing key plate 301 can be slidably lifted and lowered on the mounting frame 501 through the lifting mechanism 505.
  • a protruding key strip for lid closing is provided at the bottom of the lid closing key plate 301, so as to facilitate pressing to close the lid of the reaction tube.
  • the mounting frame 501 includes a mounting plate 502, a guide rod 503 vertically fixed on the mounting plate 502, and a limit plate 504 fixed between the guide rods 503.
  • the needle holder mechanism is fixed on the limit plate 504, and the limit plate 504 is driven by the motor 506 to slide and rise and fall along the guide rod 503.
  • the mounting frame 501 is equipped with a sensor for sensing the reaction tube on the carrier 402 and a calibration groove 10 for liquid level calibration and measuring the liquid level height of the reaction tube, so that when the liquid filling level reaches the set value, the liquid filling is automatically stopped, and the integrated module 4 is transported to the next work area platform 2 for operation and transported to the cover closing module 3 for cover closing and packaging after the liquid filling is completed.
  • a calibration tank 10 for liquid level calibration and measuring the liquid level height of the reaction tube is installed below the liquid adding integrated module 6, and a sensor for sensing the reaction tube on the carrier 402 is also installed below the liquid adding integrated module 6.
  • the calibration tank 10 is connected to the container 8 through a tank pipe, and the tank pipe is connected to a precision pump 701.
  • the tank pipe is placed in the container 8 through a straight-through joint.
  • the calibration tank 10 receives the test liquid flowing out of the liquid adding integrated module 6 by driving the motor 506 to move and measure and calibrate through the precision pump 701.
  • the calibration tank 10 and its related components are used to detect the liquid of the liquid adding integrated module 6 before the re-adding operation to ensure the accuracy and precision of the liquid adding.
  • the calibration tank 10 also has a liquid receiving and recycling function.
  • the sensor detects the status of all the liquid adding needles 601, and plays the role of calibrating the liquid discharge status of the liquid adding needles 601. That is, the machine is automatically calibrated when it is turned on, and the equipment is initialized when it is started.
  • the calibration tank 10 is driven by the motor 506 to move under the liquid adding needle 601, and the liquid adding needle 601 injects liquid into the calibration tank 10.
  • the precision pump 701 connected to the calibration tank 10 is started to pump the liquid into the container 8.
  • the sensor detects the status of all the liquid adding needles 601. If the status of the liquid adding needle 601 meets the requirements, the calibration tank 10 returns to its original position and enters the working state.
  • the control system includes a sensor, a signal transmission module, a signal processing module and a display.
  • the working area platform 2, the device and the carrier 402 are all provided with sensors.
  • the position of the reaction tube on the carrier 402 is sensed by the sensor.
  • the signal transmission module transmits the signal to the signal processing module for analysis and processing, and then controls the working area platform 2 to add liquid and release, the capping module 3 to close and release, and the conveying integrated module 4 to transport the carrier 402 according to a preset program to complete the liquid adding and capping process, thereby realizing the automation, controllable and operable effect of the overall process.
  • the left and right sides of the front surface of the shell 1 are movably connected with cabinet doors through hinges, which are convenient to open and store items.
  • the cabinet door surface is recessed with door buckles, and universal wheels 11 are installed at the bottom of the shell 1 to facilitate moving and carrying the equipment.
  • the bottom surface of the protruding key strip is horizontal, and the same side is arc-shaped, which is convenient for closing the cover and packaging.
  • the precision pump 701 can be one of a micro gear pump, a picoliter pump, a syringe pump, a plunger pump, a peristaltic pump, a piezoelectric valve, and a solenoid valve.
  • the control system adopts PLC, which can be divided into closed-loop control system and program control system. It can give full play to the function of PLC and meet the control requirements of the controlled object to the greatest extent.
  • PLC input/output module, I/O points and memory capacity
  • the optimal embodiment is that the number of the working area platforms 2 is three, including a first working area platform, a second working area platform and a third working area platform.
  • the main indicators and parameters of the nucleic acid reagent finished product production device are as follows: working power supply: AC 220V, input power: about 2 KW, ambient temperature: 21-25°C (no icing), relative humidity: ⁇ 65% (no condensation), temperature range of the first working area platform: 1-5°C (no icing), temperature range of the second working area platform: 10-35°C, temperature range of the third working area platform: -20°C-5°C, liquid addition range: 1-50 ⁇ L, liquid addition accuracy: 1-5 ⁇ L ⁇ 2%, 6-50 ⁇ L ⁇ 1%, working mode: S2, production efficiency: 4800-96000PCS/H, bottle sealing model: PCR reaction tube, positioning accuracy: 0.05MM; the PCR reaction tube used in the bottle sealing model can be 0.1, 0.2, 0.3, etc., and a single tube or multi-tube of the corresponding rule can be selected according to needs.
  • the liquid flow in the liquid adding tube 702 and the liquid flow pipeline is utilized to make the storage temperature of the reagents and raw materials scientific and reasonable; the liquid flow and the circulating flow are utilized to make the reagents and raw materials respectively achieve the purpose of mixing, the reagents have good stability, and the mixing effect is better.
  • the problem of excessive temperature difference and precipitation between the reagents and raw materials stored in the container 8 and the pipeline is solved.
  • a liquid level alarm is installed outside the pipeline for liquid flow and circulating flow to achieve the purpose of liquid level prompting.
  • the combination of the temperature control device and the liquid flow pipeline design makes the overall compatibility good.
  • the liquid adding pipe 702 enters the working state when adding liquid, and can also be in the standby state (not working) at other times; the liquid level alarm installed on the liquid flow pipeline is mainly used to play the role of liquid level prompt, prompting whether timely liquid replenishment is needed.
  • the liquid flow pipeline has three functions. The first function (starting the liquid circulation flow) is heat preservation, the second function (starting the liquid circulation flow) is mixing, and the third function (starting the liquid circulation flow) uses the pipe position difference to detect the water level in the container 8 to achieve the early warning water level, that is, to prompt the effect of liquid replenishment.
  • the heat preservation, mixing and liquid level reminder are realized through the liquid flow pipeline.
  • the tank tube of the calibration tank 10 is mainly calibrated when the machine is started or when calibration is required, and is stationary at other times; at the same time, the three tubes in this embodiment have different main functions, the liquid adding tube 702 adds liquid, the liquid flow pipeline is insulated, and the tank tube of the calibration tank 10 is used to calibrate the liquid level and recover the liquid, and the three tubes work together, especially when they are all placed in the container 8, they can further circulate and mix fully; on the other hand, the sensor is not easily contaminated if it is not placed in the container 8, and is not attached to the outer wall of the container 8 (the sensor is close to the outer wall of the container 8, which not only increases the space, but also is not conducive to temperature control).
  • the calibration tank 10 and its related components are used to detect the liquid of the liquid adding integrated module 6 before the re-adding operation to ensure the accuracy and precision of the liquid adding.
  • the calibration tank 10 also has the function of receiving and recycling liquid.
  • the sensor detects the status of all the liquid adding needles 601, and plays the role of calibrating the liquid discharge status of the liquid adding needles 601. That is, the machine is automatically calibrated, the equipment is initialized, the calibration tank 10 is driven by the motor 506 to move under the liquid adding needle 601, the liquid adding needle 601 injects liquid into the calibration tank 10, the precision pump 701 connected to the calibration tank 10 is started, and the liquid is pumped into the container 8.
  • the sensor detects the status of all the liquid adding needles 601.
  • the status of the liquid adding needle 601 meets the requirements, and the calibration tank 10 returns to the original position and enters the working state, so it has the functions of receiving liquid, flushing, and recycling. Avoid waste, save costs, no need to dismantle the machine to change tubes, save time, and improve production efficiency; the production area uses a dark room to avoid light, so that the storage temperature of reagents and raw materials is scientific and reasonable, with good stability, good mixing effect, high liquid addition accuracy, and good uniformity of the finished products, which improves the production efficiency of nucleic acid testing reagents and shortens the production time.
  • the method for using the above-mentioned automated nucleic acid reagent finished product production device comprises the following steps:
  • A Use the external power cord to connect to the power socket 104, so that the production equipment is in a powered state, press the control button 103 to control each component to enter the working state in turn, and at the same time, the data of the working state of each power-consuming component is fed back to the display screen 106 for display:
  • the transmission integrated module 4 drives the carrier 402 to move and enter the working area platform 2 in the shell 1.
  • the transmission integrated module 4 stops running, and the precision pump 701 draws the liquid in the container 8 and adds it to the reaction tube through the liquid adding needle 601 of the liquid adding integrated module 6.
  • the calibration tank 10 detects that the liquid level is at the set height, it stops adding liquid, and then the transmission integrated module 4 continues to run;
  • the whole process is controlled by the control system and operates according to the preset program. After the finished product is completed, it is automatically released to allow the carrier 402 with PCR reaction tubes at the back to automatically fill the empty position, so that no reaction tube is added without liquid.
  • the PCR reaction tube enters the lower position of the liquid adding needle 601, other working sections continue to work.
  • the liquid adding needle 601 rises and stops working, and the slide rail 401 part of the conveying integrated module 4 works.
  • the utility model adheres to scientific, regular and standardized principles, which is conducive to further improving the product quality of nucleic acid detection reagents, accelerating the production efficiency of nucleic acid detection reagent finished products, shortening the production time, and reducing the requirements for experimental facilities and equipment and personnel. It solves the common problems of difficulty in producing finished nucleic acid detection reagents in the fields of molecular diagnosis and biomedicine, solves the problem of large-scale and stable production of finished products, and further improves the automation process of nucleic acid detection reagent finished product production.

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Abstract

一种自动化核酸试剂成品制作装置，属于分子诊断、生物医药设备技术领域，包括壳体（1），壳体（1）内安装有两个以上的工作区平台（2）、合盖模块（3）和控制作业运行的控制系统，横穿工作区平台（2）和合盖模块（3）设有用于输送载具（402）的传送集成模块（4）；工作区平台（2）包括可升降的升降移动模块（5）、用于加液的加液集成模块（6）和控制加液量和温度的液控集成模块（7）；加液集成模块（6）通过升降移动模块（5）带动上升或下降进行加液，加液集成模块（6）与液控集成模块（7）连接。在保障核酸检测试剂的性能的情况下，实现了一步到位，高效制作得到核酸试剂成品的目的，减少了核酸试剂后续经过的设备，大大提高了核酸试剂的生产效率。

Description

一种自动化核酸试剂成品制作装置 技术领域
本实用新型涉及分子诊断、生物医药设备技术领域，具体为一种智能自动化核酸试剂成品制作装置。
 背景技术
核酸检测即通常所说的分子检测，实时荧光定量聚合酶链式反应技术，是最近十几年随着基因组技术而迅速崛起的新兴产业领域。
目前，核酸检测，世界各国大部分试剂生产企业提供的是大包装（半成品），生产企业利用灌装机，将核酸检测试剂灌装成大包装规格，使用时需解冻，振荡器预混后，医疗机构常用移液器或移液器工作站分装成单人份，偶见少数医疗机构采用灌装机分装成单人份，如需保持灵敏度，还需现配现用，不能反复冻融，不可长时间暴露在光线下，大包装存在的问题，显而易见，制作工艺有待提高，远远达不到生物战疫中，精准快速反应的要求，终端使用限制多。
试剂的混匀效果也会影响最终的检测结果，因此用于试剂混匀装置在整个反应过程中起着重要的作用，常规混匀方式包括搅拌、振荡和超声三种方式，其中搅拌和超声影响检测结果的可靠性和准确性，一般多采用振荡混匀的方式对试剂进行混匀，但加装温控装置兼容性差。
成品制作工艺要求极高，人工制作时稳定性差，难以量产，现实中灌装机、移液器、移液器工作站，功能单一，需要多种仪器设备的配合，难以达到检测试剂成品制作的基本要求。而且来回转移到不同的仪器设备中进行加液，增加了污染、暴露的风险，而在同一仪器中又难以实现准确加液混合，以及控制不同原材料在不同的温度，也是疑难点。
此外，基于技术局限，以不降低性能为前提，核酸检测试剂成品制作难是分子诊断、生物医药领域存在的共性问题，世界各国都缺少自动化核酸试剂成品制作装置无法规模化（百万级日产量）生产。
因此，亟待研发出一种智能自动化核酸试剂成品制作装置来解决上述问题。
 技术解决方案
本实用新型的目的在于提供一种智能自动化核酸试剂成品制作装置，在保障核酸检测试剂的性能的情况下，实现了一步到位，高效制作得到核酸试剂成品的目的。
为实现上述目的，本实用新型提供如下技术方案：
一种自动化核酸试剂成品制作装置，包括壳体，所述壳体内安装有两个以上的工作区平台、合盖模块和控制作业运行的控制系统，横穿所述工作区平台和合盖模块设有用于输送载具的传送集成模块；
所述工作区平台包括可升降的升降移动模块、用于加液的加液集成模块和控制加液量和温度的液控集成模块；
所述加液集成模块通过升降模块带动上升或下降进行加液，所述加液集成模块与所述液控集成模块连接，通过控制系统控制所述传送集成模块输送载具至工作区平台加液并在加液完成后输送至合盖模块完成合盖封装。
进一步所采取的措施是：所述液控集成模块铺设有用于保温和混匀的液流管道。
进一步所采取的措施是：所述液控集成模块包括精密泵、加液管道和温控装置，所述加液管道连通安装精密泵，所述加液管的两端分别与加液集成模块的液流入口和容器连接相通，所述液流管道紧贴加液管缠绕铺设，所述容器安装有调节容器内液体温度的温控装置。
进一步所采取的措施是：所述容器设有两个开口，所述容器的两个开口分别与加液管和液流管道连通；所述加液管道连通安装有精密泵，所述加液集成模块的液流入口与液流管道连接相通。
进一步所采取的措施是：所述容器的两个开口通过直通接头分别与加液管和液流管道连通；所述液流管道外围包裹保温材料，所述液流管道和加液通道均设有液位警示器；所述壳体底部安装有料筒，所述容器放置于料筒中。
进一步所采取的措施是：所述传送集成模块包括感应器、电机、传送带、滑轨，所述滑轨横穿壳体中部，所述传送带铺设于滑轨上，设有感应器的所述载具安装于传送带上，通过电机驱动传送带使得传送带上的载具沿滑轨移动。
进一步所采取的措施是：所述壳体包括中间较高的凸台和两端较低的平台，所述工作区平台和合盖模块依次排列位于凸台内，所述滑轨水平呈直线设置，所述传送带的两端铺设于平台顶部，其中部横穿凸台经过工作区平台和合盖模块进行加液及合盖作业。
进一步所采取的措施是：所述加液集成模块包括加液针和加液针底部用于出液的出液端口；所述升降移动模块包括针架机构、一个安装架、一个升降机构和电机；所述安装架上设有升降机构，所述针架机构可升降安装在升降机构内，所述针架机构上安装加液针，所述加液针的顶端与加液管连接，通过电机驱动所述升降机构使得针架机构上的加液针上升、下降进行加液。
进一步所采取的措施是：所述合盖模块包括合盖键板、一个安装架和一个升降机构，所述合盖键板通过升降机构可滑动升降安装在安装架上，所述合盖键板底部设有用于合盖的突出键条。
进一步所采取的措施是：所述安装架包括安装板、竖直固接在安装板上的导杆和固接在导杆之间的限位板，所述针架机构固装在限位板上，通过所述电机驱动限位板沿着导杆滑动升降，所述安装架安装有用于感应载具上的反应管的感应器和用于液面校准和测量反应管液面高度的校准槽。
进一步所采取的措施是：所述控制系统包括感应器，信号传输模块、信号处理模块和显示器，所述工作区平台、和该装置和载具上均设有感应器，通过感应器感应载具上反应管的位置，信号传输模块传输信号至信号处理模块进行分析处理后按预设程序控制工作区平台进行加液和放行、合盖模块的合盖和放行以及传送集成模块输送载具完成加液和合盖流程。
进一步所采取的措施是：所述壳体正表面的左右两侧均通过合页活动连接有柜门，所述柜门表面凹设有门扣，所述壳体底部安装有万向轮。
进一步所采取的措施是：所述突出键条的底面呈水平，同向一侧呈弧形。
进一步所采取的措施是：所述精密泵可采用微型齿轮泵、皮升泵、注射泵、柱塞泵、蠕动泵、压电阀、电磁阀中的一种。
进一步所采取的措施是：所述控制系统采用PLC，可分为闭环控制系统和程序控制系统，可充分发挥PLC的功能，最大限度地满足被控对象的控制要求，选择PLC、输入/输出模块、I/O点数和内存容量时，已经适当留有裕量。
进一步所采取的措施是：所述加液集成模块下方安装有用于液面校准和测量反应管液面高度的校准槽，所述加液集成模块下方还安装有用于感应载具上的反应管的感应器。
进一步所采取的措施是：所述校准槽通过槽管与容器连通，所述槽管衔接有精密泵，所述槽管通过直通接头放入容器中，所述校准槽以电机驱动位移接收加液集成模块流出的测试液并通过精密泵测量校准。
通过校准槽及其相关部件对加液集成模块的液体再加液作业前进行检测，保障加液的准确和精密性，同时校准槽还具有接液回收功能，感应器检测所有加液针状态，起到校准加液针出液状态的作用。即开机自动校准，设备启动初始化，校准槽受电机驱动位移至加液针下，加液针注液进入校准槽，校准槽连接的精密泵启动，将液体抽至容器内，感应器检测所有加液针状态，加液针状态符合要求，校准槽返回原位，进入工作状态。
进一步所采取的措施是：所述控制系统包括感应器，信号传输模块、信号处理模块和显示器，所述工作区平台、和该装置和载具上均设有感应器，通过感应器感应载具上反应管的位置，信号传输模块传输信号至信号处理模块进行分析处理后按预设程序控制工作区平台进行加液和放行、合盖模块的合盖和放行以及传送集成模块输送载具完成加液和合盖流程。
进一步所采取的措施是：所述壳体正表面的左右两侧均通过合页活动连接有柜门，所述柜门表面凹设有门扣，所述壳体底部安装有万向轮；所述突出键条的底面呈水平，同向一侧呈弧形；所述精密泵可采用微型齿轮泵、皮升泵、注射泵、柱塞泵、蠕动泵、压电阀、电磁阀中的一种；所述控制系统采用PLC系统。
进一步所采取的措施是：其中所述工作区平台的数量为三个，包括第一工作区平台、第二工作区平台和第三工作区平台：
进一步所采取的措施是：该核酸试剂成品制作装置的主要指标和参数如下，工作电源:AC 220V，输入功率:约2 KW，环境温度:21～25℃ (无结冰)，相对湿度:≤65%(无结露)，第一工作区平台温度范围:1～5℃(无结冰)，第二工作区平台温度范围:10～35℃，第三工作区平台温度范围: -20℃～5℃，加液范围:1～50μL，加液精度:1～5μL±2%，6～50μL±1%，工作制式:S2，生产效率;4800～96000PCS/H，封瓶型号:PCR反应管，定位精度:0.05MM。
 有益效果
与现有技术相比，本实用新型的有益效果如下：
通过在壳体内安装有工作区平台、合盖模块和控制作业运行的控制系统，横穿所述工作区平台和合盖模块设置用于输送载具的传送集成模块，使得反应管可以放置在载具上，通过控制系统控制传送集成模块输送载具上的反应管到工作区平台进行添加各种原材料液体，并在完成加液后继续输送至合盖模块完成合盖封装，从而自动完成核酸检测试剂材料的配置，直接制作得到成品，实现了一步到位制作核酸试剂成品，不需要过多仪器设备的参与和繁琐严苛的制备操作；同时在壳体内完成制作，避免了暴露、污染的风险，避光制作，更好的保障核酸检测试剂的性能，还有本技术方案操作简便，自动化，效率高，还能够实现规模化（百万级日产量）生产。
 附图说明
图1为本实用新型外部结构的立体图；
图2为本实用新型结构的仰视图；
图3为本实用新型内部结构的俯视图；
图4为本实用新型内部结构的正视图；
图5为本实用新型工作区平台结构的正视图；
图6为本实用新型合盖模块结构的正视图；
图7为本实用新型一实施例的核酸试剂成品制作装置的工作流程图。
图中：1、壳体；101、凸台；102、平台；103、控制按键；104、电源插口；105、警示灯；106、显示屏；2、工作区平台；3、合盖模块；301、合盖键板；4、传送集成模块；401、滑轨；402、载具；5、升降移动模块；501、安装架；502、安装板；503、导杆；504、限位板；505、升降机构；506、电机；6、加液集成模块；601、加液针；7、液控集成模块；701、精密泵；702、加液管；8、容器；9、料筒；10、校准槽；11、万向轮。
 本发明的最佳实施方式
下面结合附图和具体实施例对本实用新型作进一步的详细说明。本说明书中所引用的如“上”、“内”、“中”、“左”、“右”及“一”等的用语，亦仅为便于叙述的明了，而非用以限定本实用新型可实施的范围，其相对关系的改变或调整，在无实质变更技术内容下，当亦视为本实用新型可实施的范畴。
以下实施例请参阅图1-图6，一种自动化核酸试剂成品制作装置，包括壳体1，所述壳体1内安装有两个以上的工作区平台2、合盖模块3和控制作业运行的控制系统，横穿所述工作区平台2和合盖模块3设有用于输送载具402的传送集成模块4；所述工作区平台2包括可升降的升降移动模块5、用于加液的加液集成模块6和控制加液量和温度的液控集成模块7；所述加液集成模块6通过升降模块带动上升或下降进行加液，所述加液集成模块6与所述液控集成模块7连接，通过所述传送集成模块4输送载具402至工作区平台2加液并在加液完成后输送至合盖模块3完成合盖封装。
通过在壳体1内安装有工作区平台2、合盖模块3和控制作业运行的控制系统，横穿所述工作区平台2和合盖模块3设置用于输送载具402的传送集成模块4，使得反应管可以放置在载具402上，通过控制系统控制传送集成模块4输送载具402上的反应管到工作区平台2进行添加各种原材料液体，并在完成加液后继续输送至合盖模块3完成合盖封装，从而自动完成核酸检测试剂材料的配置，直接制作成成品，实现了一步到位制作核酸试剂成品过多仪器设备的参与和繁琐严苛的制备，同时在壳体1内完成制作，避免了暴露、污染的风险，更能保障核酸检测试剂的性能，还有本技术方案操作简便，自动化，效率高，还能够实现规模化（百万级日产量）生产。
所述液控集成模块7铺设有用于保温和混匀的液流管道，同时液流管道利用液体流动、循环流动，使试剂和原材料各自达到混匀目的。
通过在液控集成模块7铺设用于保温的液流管道，该巧妙设计使得加液管702中流动的液体也能够得到保温的效果，实现了加液过程中也能够保障液流的温度，而且液流管道的形式避免引入复杂的加温控制装置，而且兼容性高，不同的工作区平台2完全可以控制在不同的温度区间，保障核酸试剂的配备过程和性能。
所述液控集成模块7包括精密泵701、加液管702道和温控装置，所述加液管702道连通安装精密泵701，从而可精准控制加液量，所述加液管702的两端分别与加液集成模块6的液流入口和容器8连接相通，所述液流管道紧贴加液管702缠绕铺设，使得保温效果更佳，而且不会影响其他工作区的温度，所述容器8安装有调节容器8内液体温度的温控装置，通过容器8更好的调节液体的温度，方便加热或制冷控温。
所述容器8设有两个开口，所述容器8的两个开口分别与加液管702和液流管道连通；所述加液管702道连通安装有精密泵701，所述加液集成模块6的液流入口与液流管道连接相通，这样一来，既能够更好的保持加液管702内液流的温度，同时不需要额外再液流管道通入其他的液流，且保温效果更佳，而再有需要的情况下还能够方便补充液流。
所述容器8的两个开口通过直通接头分别与加液管702和液流管道连通；所述液流管道外围包裹保温材料，实现更佳的保温效果，所述液流管道和加液通道均设有液位警示器，从而可提醒液流液位；所述壳体1底部安装有料筒9，所述容器8放置于料筒9中。
所述传送集成模块4包括感应器、电机506、传送带、滑轨401，所述滑轨401横穿壳体1中部，所述传送带铺设于滑轨401上，设有感应器的所述载具402安装于传送带上，通过电机506驱动传送带使得传送带上的载具402沿滑轨401移动，从而实现载具402的输送，实现自动流水作业制备成成品。
所述壳体1包括中间较高的凸台101和两端较低的平台102，所述工作区平台2和合盖模块3依次排列位于凸台101内，所述滑轨401水平呈直线设置，所述传送带的两端铺设于平台102顶部，从而方便操作放置反应管到载具402上以及在末端取出合盖封装好的反应管成品，其中部横穿凸台101经过工作区平台2和合盖模块3进行加液及合盖作业，保障了中间加液过程中避光，有相对闭合的空间。
所述加液集成模块6包括加液针601和加液针601底部用于出液的出液端口；所述升降移动模块5包括针架机构、一个安装架501、一个升降机构505和电机506；所述安装架501上设有升降机构505，所述针架机构可升降安装在升降机构505内，所述针架机构上安装加液针601，所述加液针601的顶端与加液管702连接，通过电机506驱动所述升降机构505使得针架机构上的加液针601上升、下降进行加液，从而可灵活自动的进行加液。
所述合盖模块3包括合盖键板301、一个安装架501和一个升降机构505，所述合盖键板301通过升降机构505可滑动升降安装在安装架501上，所述合盖键板301底部设有用于合盖的突出键条，从而方便按压，将反应管的盖子合上。
所述安装架501包括安装板502、竖直固接在安装板502上的导杆503和固接在导杆503之间的限位板504，所述针架机构固装在限位板504上，通过所述电机506驱动限位板504沿着导杆503滑动升降，所述安装架501安装有用于感应载具402上的反应管的感应器和用于液面校准和测量反应管液面高度的校准槽10，从而在加液位达到设定值时，自动停止加液，传送集成模块4输送到下一工作区平台2进行作业以及完成加液后输送到合盖模块3进行合盖封装。
所述加液集成模块6下方安装有用于液面校准和测量反应管液面高度的校准槽10，所述加液集成模块6下方还安装有用于感应载具402上的反应管的感应器。其中，所述校准槽10通过槽管与容器8连通，所述槽管衔接有精密泵701，所述槽管通过直通接头放入容器8中，所述校准槽10以电机506驱动位移接收加液集成模块6流出的测试液并通过精密泵701测量校准。
通过校准槽10及其相关部件对加液集成模块6的液体再加液作业前进行检测，保障加液的准确和精密性，同时校准槽10还具有接液回收功能，感应器检测所有加液针601状态，起到校准加液针601出液状态的作用。即开机自动校准，设备启动初始化，校准槽10受电机506驱动位移至加液针601下，加液针601注液进入校准槽10，校准槽10连接的精密泵701启动，将液体抽至容器8内，感应器检测所有加液针601状态，加液针601状态符合要求，校准槽10返回原位，进入工作状态。
所述控制系统包括感应器，信号传输模块、信号处理模块和显示器，所述工作区平台2、和该装置和载具402上均设有感应器，通过感应器感应载具402上反应管的位置，信号传输模块传输信号至信号处理模块进行分析处理后按预设程序控制工作区平台2进行加液和放行、合盖模块3的合盖和放行以及传送集成模块4输送载具402完成加液和合盖流程，实现整体过程的自动化和可控可操作的效果。
所述壳体1正表面的左右两侧均通过合页活动连接有柜门，方便打开，存放物品，所述柜门表面凹设有门扣，所述壳体1底部安装有万向轮11，方便移动搬运该设备。
所述突出键条的底面呈水平，同向一侧呈弧形，方便合盖封装。所述精密泵701可采用微型齿轮泵、皮升泵、注射泵、柱塞泵、蠕动泵、压电阀、电磁阀中的一种。
所述控制系统采用PLC，可分为闭环控制系统和程序控制系统，可充分发挥PLC的功能，最大限度地满足被控对象的控制要求，选择PLC、输入/输出模块、I/O点数和内存容量时，已经适当留有裕量。
其中，最优实施例是，所述工作区平台2的数量为三个，包括第一工作区平台、第二工作区平台和第三工作区平台。
该核酸试剂成品制作装置的主要指标和参数如下，工作电源:AC 220V，输入功率:约2 KW，环境温度:21～25℃ (无结冰)，相对湿度:≤65%(无结露)，第一工作区平台温度范围:1～5℃(无结冰)，第二工作区平台温度范围:10～35℃，第三工作区平台温度范围: -20℃～5℃，加液范围:1～50μL，加液精度:1～5μL±2%，6～50μL±1%，工作制式:S2，生产效率;4800～96000PCS/H，封瓶型号:PCR反应管，定位精度:0.05MM；其中封瓶型号采用的PCR反应管可以为0.1、0.2、0.3等，根据需要选择相应规则的单联管或者多联管即可。
本实施例，利用加液管702和液流管道内的液体流动，特别是实现了循环流动，使试剂和原材料贮存温度科学合理；利用液体流动、循环流动，使试剂和原材料各自达到混匀目的，试剂的稳定性好，混匀效果更佳，同时解决了试剂、原材料贮存在容器8内至管道温差过大和沉淀难题，在液体流动、循环流动的管道外加装液位警示器，达到液位提示目的，加装温控装置和液流管道设计结合使得整体兼容性好。
其中，加液管702道，加液进入工作状态，其它时间也可以是待机状态（不工作的）；液流管道上安装的液位警示器，主要用于起到液位提示作用，提示是否需要及时补液，液流管道具有3种作用，第一个功能（开机液体循环流动）保温，第二个功能（开机液体循环流动）混匀，第三个功能（开机液体循环流动）利用管位落差，探测容器8内的水位，达到预警水位，即提示补液的效果，通过液流管道实现了保温，混匀和液位提示的目的；而校准槽10的槽管，主要在开机启动时进行校准的或者需要校准时启动，其它时间是静止状态；同时本实施例中的三种管主要功能不同，加液管702加液，液流管道保温，校准槽10的槽管用于校准液面和回收液体，而三种管共同作用特别是都放在容器8内时，又能起到进一步充分循环混匀；另而感应器不放入容器8不易污染，不贴在容器8外壁（感应器紧贴容器8外壁，除增加空间外，还不利于温度的控制）。
本实施例通过校准槽10及其相关部件对加液集成模块6的液体再加液作业前进行检测，保障加液的准确和精密性，同时校准槽10还具有接液回收功能，感应器检测所有加液针601状态，起到校准加液针601出液状态的作用。即开机自动校准，设备启动初始化，校准槽10受电机506驱动位移至加液针601下，加液针601注液进入校准槽10，校准槽10连接的精密泵701启动，将液体抽至容器8内，感应器检测所有加液针601状态，加液针601状态符合要求，校准槽10返回原位，进入工作状态，因此具有接液，冲冼，回收的作用。避免浪费，节约了成本，无需拆机换管，节约了时间，提高了生产效率；制作区采用暗室避光，使试剂和原材料贮存温度科学合理，稳定性好、混匀状态效果好、加液精度高，制作的成品均一性好，提高了核酸检测试剂成品制作效率，缩短了制作时间。
参阅图7的工作流程：上述一种自动化核酸试剂成品制作装置的使用方法，包括如下步骤：
A：利用外界电源线与电源插口104进行对接，使得制作设备处于有电状态，按下控制按键103控制各部件依次进入工作状态，同时各用电部件工作状态下的数据反馈给显示屏106进行显示：
B：将PCR反应管放置于传送集成模块4起始段的载具402内，通过传送集成模块4的运行，带动载具402移动，进入壳体1内的工作区平台2，当工作区平台2内的感应器检测到PCR反应管后，传送集成模块4停止运行，精密泵701将容器8内的液体抽出经过加液集成模块6的加液针601加入反应管内，而校准槽10，检测到液位处于设定高度时，停止加液，接着传送集成模块4继续运行；
C：当加液完成，反应管到达合盖装置工位时，合盖模块3运行，合盖键板301下移，对反应管进行合盖密封，密封结束后传送集成模块4继续运行，送出合盖模块3至传送集成模块4末尾平台102处，即可取出成品。
整个过程通过控制系统控制操作，按预设程序流水作业，完成制作成品后，自动放行，让后面的装有PCR反应管载具402自动补空位，实现无反应管不加液，当PCR反应管进入加液针601下位置时，其他工作段继续工作，当PCR反应管离开时，加液针601升起停止工作，传送集成模块4的滑轨401部分工作。
本实用新型坚持科学化、规范化、标准化，有利于进一步提升核酸检测试剂产品质量，加快核酸检测试剂成品制作效率，缩短制作时间，能够降低实验设施设备和人员要求，解决了分子诊断、生物医药领域核酸检测试剂成品制作难的共性问题，解决了大规模稳定制作成品难题，进一步提升了核酸检测试剂成品制作自动化进程，有利于促进中游核酸检测试剂生产企业，具备成品制作能力，具备（百万级）大规模量产能力，全面满足生物战疫，对核酸检测试剂成品用量的需求。
本实用新型可以以多种不同的方案来实现，并不限于本文所描述的实施方案，尽管已经示出和描述了本技术的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本技术的原理和精神的情况可以对这些实施例进行多种变化、修改、替换和变型，均落在本实用新型权利保护范围之内。

Claims (15)

  1. 一种自动化核酸试剂成品制作装置，其特征在于：包括壳体，所述壳体内安装有两个以上的工作区平台、合盖模块和控制作业运行的控制系统，横穿所述工作区平台和合盖模块设有用于输送载具的传送集成模块；
    所述工作区平台包括可升降的升降移动模块、用于加液的加液集成模块和控制加液量和温度的液控集成模块；
    所述加液集成模块通过升降模块带动上升或下降进行加液，所述加液集成模块与所述液控集成模块连接，通过控制系统控制所述传送集成模块输送载具至工作区平台加液并在加液完成后输送至合盖模块完成合盖封装。
  2. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述液控集成模块铺设有用于保温和混匀的液流管道；所述液控集成模块包括精密泵、加液管道和温控装置，所述加液管道连通安装精密泵，所述加液管的两端分别与加液集成模块的液流入口和容器连接相通，所述液流管道紧贴加液管缠绕铺设，所述容器安装有调节容器内液体温度的温控装置。
  3. 根据权利要求2所述的一种自动化核酸试剂成品制作装置，其特征在于：所述容器设有两个开口，所述容器的两个开口分别与加液管和液流管道连通；所述加液管道连通安装有精密泵，所述加液集成模块的液流入口与液流管道连接相通。
  4. 根据权利要求3所述的一种自动化核酸试剂成品制作装置，其特征在于：所述容器的两个开口通过直通接头分别与加液管和液流管道连通；所述液流管道外围包裹保温材料，所述液流管道和加液通道均设有液位警示器；所述壳体底部安装有料筒，所述容器放置于料筒中。
  5. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述传送集成模块包括感应器、电机、传送带、滑轨，所述滑轨横穿壳体中部，所述传送带铺设于滑轨上，设有感应器的所述载具安装于传送带上，通过电机驱动传送带使得传送带上的载具沿滑轨移动。
  6. 根据权利要求5所述的一种自动化核酸试剂成品制作装置，其特征在于：所述壳体包括中间较高的凸台和两端较低的平台，所述工作区平台和合盖模块依次排列位于凸台内，所述滑轨水平呈直线设置，所述传送带的两端铺设于平台顶部，其中部横穿凸台经过工作区平台和合盖模块进行加液及合盖作业。
  7. 根据权利要求2所述的一种自动化核酸试剂成品制作装置，其特征在于：所述加液集成模块包括加液针和加液针底部用于出液的出液端口；所述升降移动模块包括针架机构、一个安装架、一个升降机构和电机；所述安装架上设有升降机构，所述针架机构可升降安装在升降机构内，所述针架机构上安装加液针，所述加液针的顶端与加液管连接，通过电机驱动所述升降机构使得针架机构上的加液针上升、下降进行加液。
  8. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述合盖模块包括合盖键板、一个安装架和一个升降机构，所述合盖键板通过升降机构可滑动升降安装在安装架上，所述合盖键板底部设有用于合盖的突出键条。
  9. 根据权利要求8所述的一种自动化核酸试剂成品制作装置，其特征在于：所述安装架包括安装板、竖直固接在安装板上的导杆和固接在导杆之间的限位板，所述针架机构固装在限位板上，通过所述电机驱动限位板沿着导杆滑动升降。
  10. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述加液集成模块下方安装有用于液面校准和测量反应管液面高度的校准槽，所述加液集成模块下方还安装有用于感应载具上的反应管的感应器。
  11. 根据权利要求10所述的一种自动化核酸试剂成品制作装置，其特征在于：所述校准槽通过槽管与容器连通，所述槽管衔接有精密泵，所述槽管通过直通接头放入容器中，所述校准槽以电机驱动位移接收加液集成模块流出的测试液并通过精密泵测量校准。
  12. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述控制系统包括感应器，信号传输模块、信号处理模块和显示器，所述工作区平台、和该装置和载具上均设有感应器，通过感应器感应载具上反应管的位置，信号传输模块传输信号至信号处理模块进行分析处理后按预设程序控制工作区平台进行加液和放行、合盖模块的合盖和放行以及传送集成模块输送载具完成加液和合盖流程。
  13. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述壳体正表面的左右两侧均通过合页活动连接有柜门，所述柜门表面凹设有门扣，所述壳体底部安装有万向轮；所述突出键条的底面呈水平，同向一侧呈弧形；所述精密泵可采用微型齿轮泵、皮升泵、注射泵、柱塞泵、蠕动泵、压电阀、电磁阀中的一种；所述控制系统采用PLC系统。
  14. 根据权利要求1所述的一种自动化核酸试剂成品制作装置，其特征在于：所述工作区平台的数量为三个，包括第一工作区平台、第二工作区平台和第三工作区平台。
  15. 根据权利要求14所述的一种自动化核酸试剂成品制作装置，其特征在于：该核酸试剂成品制作装置的主要指标和参数如下，工作电源:AC 220V，输入功率:约2 KW，环境温度:21～25℃ (无结冰)，相对湿度:≤65%(无结露)，第一工作区平台温度范围:1～5℃(无结冰)，第二工作区平台温度范围:10～35℃，第三工作区平台温度范围: -20℃～5℃，加液范围:1～50μL，加液精度:1～5μL±2%，6～50μL±1%，工作制式:S2，生产效率;4800～96000PCS/H，封瓶型号:PCR反应管，定位精度:0.05MM。
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