WO2024064962A3 - Immuno-pcr and next generation sequencing for precise antigen quantitation - Google Patents

Immuno-pcr and next generation sequencing for precise antigen quantitation Download PDF

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Publication number
WO2024064962A3
WO2024064962A3 PCT/US2023/075041 US2023075041W WO2024064962A3 WO 2024064962 A3 WO2024064962 A3 WO 2024064962A3 US 2023075041 W US2023075041 W US 2023075041W WO 2024064962 A3 WO2024064962 A3 WO 2024064962A3
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WO
WIPO (PCT)
Prior art keywords
immuno
pcr
next generation
generation sequencing
antigen quantitation
Prior art date
Application number
PCT/US2023/075041
Other languages
French (fr)
Other versions
WO2024064962A2 (en
Inventor
Solomon STONEBLOOM
Original Assignee
Slingshot Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Slingshot Biosciences, Inc. filed Critical Slingshot Biosciences, Inc.
Publication of WO2024064962A2 publication Critical patent/WO2024064962A2/en
Publication of WO2024064962A3 publication Critical patent/WO2024064962A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to polymer beads comprising (i) biomarkers and (ii) nucleic acids comprising: (a) a primer binding site; and (b) a unique molecular identifier; wherein the nucleic acids are bound to the biomarker; and wherein each nucleic acid on the polymer bead comprises a different UMI. The present disclosure also provides methods for quantitatively measuring biomarker concentration in a sample.
PCT/US2023/075041 2022-09-23 2023-09-25 Immuno-pcr and next generation sequencing for precise antigen quantitation WO2024064962A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263376965P 2022-09-23 2022-09-23
US63/376,965 2022-09-23

Publications (2)

Publication Number Publication Date
WO2024064962A2 WO2024064962A2 (en) 2024-03-28
WO2024064962A3 true WO2024064962A3 (en) 2024-05-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/075041 WO2024064962A2 (en) 2022-09-23 2023-09-25 Immuno-pcr and next generation sequencing for precise antigen quantitation

Country Status (1)

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WO (1) WO2024064962A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180088112A1 (en) * 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10328160B2 (en) * 2007-08-14 2019-06-25 The Regents Of The University Of California Hollow silica nanospheres and methods of making same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10328160B2 (en) * 2007-08-14 2019-06-25 The Regents Of The University Of California Hollow silica nanospheres and methods of making same
US20180088112A1 (en) * 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DIDIER FALCONNET, SHE JOSEPH, TORNAY RAPHAËL, LEIMGRUBER ELISA, BERNASCONI DAVID, LAGOPOULOS LUCIENNE, RENAUD PHILIPPE, DEMIERRE N: "Rapid, Sensitive and Real-Time Multiplexing Platform for the Analysis of Protein and Nucleic-Acid Biomarkers", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 87, no. 3, 3 February 2015 (2015-02-03), US , pages 1582 - 1589, XP055254544, ISSN: 0003-2700, DOI: 10.1021/ac502741c *
NUR FAEZAH IBADAT: "Synthesis and Characterization of Polymeric Microspheres Template for a Homogeneous and Porous Monolith", POLYMERS, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (M DP I) AG., CH, vol. 13, no. 21, CH , pages 3639, XP093167777, ISSN: 2073-4360, DOI: 10.3390/polym13213639 *
VIGNESHWARAN MANI, BHASKARA V CHIKKAVEERAIAH, JAMES F RUSLING: "Magnetic particles in ultrasensitive biomarker protein measurements for cancer detection and monitoring", EXPERT OPINION ON MEDICAL DIAGNOSTICS, INFORMA HEALTHCARE, GB, vol. 5, no. 5, 1 September 2011 (2011-09-01), GB , pages 381 - 391, XP055277201, ISSN: 1753-0059, DOI: 10.1517/17530059.2011.607161 *

Also Published As

Publication number Publication date
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