WO2023212852A2 - 一种利用腹腔神经节损毁术在提高机体抗压力上的应用 - Google Patents

一种利用腹腔神经节损毁术在提高机体抗压力上的应用 Download PDF

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Publication number
WO2023212852A2
WO2023212852A2 PCT/CN2022/090900 CN2022090900W WO2023212852A2 WO 2023212852 A2 WO2023212852 A2 WO 2023212852A2 CN 2022090900 W CN2022090900 W CN 2022090900W WO 2023212852 A2 WO2023212852 A2 WO 2023212852A2
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Prior art keywords
stress
surgery
celiac
destruction
mice
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PCT/CN2022/090900
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English (en)
French (fr)
Inventor
刘欣安
景晓源
巨富荣
蒋蓝萍
贾淑慧
王立平
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M21/00—Other devices or methods to cause a change in the state of consciousness; Devices for producing or ending sleep by mechanical, optical, or acoustical means, e.g. for hypnosis

Definitions

  • the invention belongs to the field of biomedical technology, and specifically relates to the application of celiac ganglion destruction surgery to improve the body's resistance to stress.
  • NCPB Celiac plexus block
  • NCPB used a blind puncture method, which had poor results.
  • imaging techniques has become increasingly widespread in recent years, but the impact of celiac ganglion destruction on emotion-related mental behaviors is still unclear.
  • the application is characterized in that the use of celiac ganglion destruction surgery significantly increases the body's pressure stress adaptability.
  • the application is characterized in that the use of celiac ganglion destruction surgery significantly increases the stress level of the body.
  • the present invention can greatly improve the body's resistance to pressure through celiac ganglion destruction surgery.
  • Celiac plexus destruction has the potential to relieve stress-related anxiety.
  • Figure 1 shows the location of the mouse celiac ganglion
  • Figure 2 shows the results of mouse behavioral tests
  • FIG. 3 shows the comparison results of stress hormone levels in mice.
  • Celiac ganglion damage surgery physically destroy the celiac ganglion of mice and interrupt the nerve connection between the brain and intestines;
  • Celiac ganglion damage surgery Weigh the mice and anesthetize them with sodium pentobarbital (80 mg/kg). Prepare sterilized surgical instruments, remove the hair from the abdomen of the mouse, and place it on a sterilized surgical pad. Use small scissors to open the mouse's abdominal cavity, cover the abdominal cavity with a surgical drape, and pull out the intestines and other organs and place them on the On a moist surgical drape, search for the celiac ganglion under a dissecting microscope so that the celiac ganglion is clearly exposed. Use small scissors to cut the ganglion. Then, use absorbable sutures to sew the lining of the mouse's abdominal cavity and skin. For the mice in the sham operation group, except for the destruction of the celiac ganglia, other procedures remained unchanged. After the operation, the mice were returned to the cage to recover for about 10 days before conducting other experiments.
  • mice After 10 days, the mice were subjected to open field experiments and black and white box experiments to observe the stress resistance performance of the mice. At the same time, we also paid attention to the changes in hormone levels in the two groups of mice.
  • Figure 1 shows the location of the mouse celiac ganglion.
  • the mouse celiac ganglion is removed and the behavioral test is performed after the mouse recovers for about 10 days.
  • the results are shown in Figure 2. It was found that the stress adaptability of mice has changed significantly.
  • the number of entries in the middle area of mice with celiac ganglia removed was significantly increased compared with mice in the control group, indicating that Mice with celiac ganglia removed showed a pronounced anxiolytic state.
  • the moving distance of mice with celiac ganglia removed in the white box increased significantly compared with mice in the control group, and showed a decrease in anxiety levels.

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  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Acoustics & Sound (AREA)
  • Psychology (AREA)
  • Engineering & Computer Science (AREA)
  • Anesthesiology (AREA)
  • Physics & Mathematics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Description

一种利用腹腔神经节损毁术在提高机体抗压力上的应用 技术领域
本发明属于生物医学技术领域，具体涉及一种利用腹腔神经节损毁术在提高机体抗压力上的应用。
背景技术
顽固性腹痛严重影响上腹部中晚期癌症及慢性疼痛患者的生活质量，按照WHO的镇痛阶梯原则，药物镇痛治疗是最常见的缓解方法，但药物止痛效果差、成瘾性及不良反应等可能降低患者生活质量。腹腔神经丛毁损术(neurolyticc eliac plexus block，NCPB)通过对腹腔神经丛注射神经破坏药以破环神经，阻断疼痛传导，可有效缓解疼痛并减少镇痛药物的使用。近年来，世界卫生组织癌症缓解计划已经推荐NCPB位缓解上腹部癌症患者疼痛的方式。NCPB可通过化学方法或物理方法毁损腹腔神经丛，从而缓解疼痛。该方法适用于合理药物治疗疼痛效果欠佳或无效的上腹部中晚期癌症患者或慢性疼痛的患者。早期NCPB采用盲刺方法，效果差。近几年采用影像学技术日益广泛，但是腹腔神经节损毁术对于情绪相关的精神行为的影响尚不清楚。
目前研究通过腹腔神经节去除手术，观察是否可以缓解小鼠的焦虑情绪还没有探讨过；我们通过实施腹腔神经节损毁手术后，对小鼠进行焦虑相关行为学测试，探究小鼠的压力应激适应力的改变。
发明内容
针对上述现有技术中存在的缺陷，本发明的目的在于设计提供一种利用腹腔神经节损毁术在提高机体抗压力上的应用。
为了实现上述目的，本发明采用了以下技术方案：
一种利用腹腔神经节损毁术在提高机体抗压力上的应用。
所述的应用，其特征在于所述利用腹腔神经节损毁术使机体的压力应激适应力显著增加。
所述的应用，其特征在于所述利用腹腔神经节损毁术使机体的应激水平显著增加。
利用腹腔神经节损毁术在缓解机体压力及压力相关情绪上的应用。
本发明应用通过腹腔神经节损毁术可以大大提高机体抗压力。腹腔神经丛毁损术有潜力用于缓解压力相关的焦虑情绪。
附图说明
图1为小鼠腹腔神经节位置图；
图2为小鼠行为学测试结果；
图3为小鼠应激激素水平比较结果。
具体实施方式
以下将通过附图和实施例对本发明作进一步说明。
实施例1：
1、腹腔神经节(Celiac ganglion)损毁手术：物理损毁小鼠腹腔神经节，打断脑与肠道的神经连接；
2、待小鼠恢复10天左右进行行为学测试，观察行为的变化情况。
腹腔神经节(Celiac ganglion)损毁手术：称量小鼠体重，使用戊巴比妥钠(80mg/kg)麻醉小鼠。准备好灭菌的手术器械，将小鼠腹部的毛发脱除干净，放在灭菌手术垫上，使用小剪刀将小鼠腹腔打开，腹腔周围盖上手术单，将肠道等器官拉出放置于湿润的手术单上，在解剖显微镜下寻找腹腔神经节，使腹腔神经节清晰得暴露出来，用小剪刀剪断神经节，然后，使用可吸收缝合针线将小鼠腹腔内层和皮肤进行缝合。对于假手术组小鼠，除了腹腔神经节损毁外，其他流程保持不变，手术结束后，将小鼠放回笼中恢复10天左右再进行其他实验。
10天后小鼠进行旷场实验以及黑白箱实验测试，观察小鼠的抗压表现。同时也关注到两组小鼠体内的激素水平的变化。
如图1所示为小鼠腹腔神经节位置，本发明通过去除小鼠腹腔神经节，待小鼠恢复10天左右进行行为学测试。结果如图2所示发现小鼠的压力应激适应力发生显著改变，在旷场实验中，去除腹腔神经节的小鼠与对照组小鼠相比，在中间区域进入次数显著性增加，表明去除腹腔神经节的小鼠表现出了明显的抗焦虑状态。黑白箱实验测试中，去除腹腔神经节的小鼠在白箱的移动距离与对照组小鼠相比显著性增加，表现出焦虑水平下降。同时，如图3可看出去除腹腔神经节的小鼠与对照组小鼠相比，体内的应激激素水平显著性增加。证明了在小鼠模型上腹腔神经节损毁术可以大大提高机体抗压力。腹腔神经丛毁损术有潜力用于缓解压力相关的焦虑情绪。

Claims (4)

  1. 一种利用腹腔神经节损毁术在提高机体抗压力上的应用。
  2. 如权利要求1所述的应用，其特征在于所述利用腹腔神经节损毁术使机体的压力应激适应力显著增加。
  3. 如权利要求1所述的应用，其特征在于所述利用腹腔神经节损毁术使机体的应激水平显著增加。
  4. 利用腹腔神经节损毁术在缓解机体压力及压力相关情绪上的应用。
PCT/CN2022/090900 2022-05-05 2022-05-05 一种利用腹腔神经节损毁术在提高机体抗压力上的应用 Ceased WO2023212852A2 (zh)

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