WO2023210597A1 - 試料採取装置 - Google Patents

試料採取装置 Download PDF

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Publication number
WO2023210597A1
WO2023210597A1 PCT/JP2023/016157 JP2023016157W WO2023210597A1 WO 2023210597 A1 WO2023210597 A1 WO 2023210597A1 JP 2023016157 W JP2023016157 W JP 2023016157W WO 2023210597 A1 WO2023210597 A1 WO 2023210597A1
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WIPO (PCT)
Prior art keywords
sample
sewage
section
absorber
sample collection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2023/016157
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English (en)
French (fr)
Japanese (ja)
Inventor
誠 八十島
公昭 嶽盛
ふみ 醍醐
基 柴山
聖人 本田
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Shimadzu Techno Research Inc
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Shimadzu Techno Research Inc
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Priority to JP2024517322A priority Critical patent/JP7782776B2/ja
Publication of WO2023210597A1 publication Critical patent/WO2023210597A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

Definitions

  • the present invention relates to a sample collection device.
  • sewage epidemiology attempts to understand trends in infectious disease outbreaks by measuring virus concentrations in sewage, including human urine and feces.
  • Many results from sewage epidemiology have been reported in the prevention of infections caused by norovirus and poliovirus, and furthermore, the results of sewage epidemiology have been reported in the prevention of infections caused by the novel coronavirus (SARS-CoV-2), which causes the novel coronavirus disease (COVID-19), which has become increasingly widespread in recent years. 2) is also considered to be applicable.
  • SARS-CoV-2 novel coronavirus
  • COVID-19 novel coronavirus disease
  • samples are collected from sewage discharged from facilities where many people stay, such as hospitals or nursing homes, and tested for the presence of infectious pathogens such as viruses in the samples. This is also used to identify infected or potentially infected persons within the facility.
  • the present invention has been made in view of the above points, and its purpose is to collect a sample from sewage and examine whether or not a detection target such as a pathogen exists in the sample.
  • the object of the present invention is to reduce the effort and cost involved in collecting samples from sewage.
  • the sample collection device which has been made to solve the above problems, includes: a sample collection unit that collects samples from sewage facilities located at the sampling site; a pretreatment unit that performs predetermined pretreatment on the sample; a refrigerator that refrigerates a sample container containing the sample after the pretreatment; a transfer unit that transfers the sample container to the refrigerator; It has the following.
  • the sewage inspection method according to the present invention includes: a step of collecting a sample from a sewage facility located at the sampling site; a step of subjecting the sample to a predetermined pretreatment at the sampling location; refrigerating the sample after the pretreatment at the collection site; transporting the refrigerated sample from the collection site to a testing institution; an inspection step of inspecting whether a detection target exists in the sample at the inspection institution; It has the following.
  • sample collection device and sewage testing method of the present invention having the above-described configuration, when collecting a sample from sewage and testing whether a detection target such as a pathogen exists in the sample, The effort and cost associated with sample collection can be reduced.
  • FIG. 1 is a schematic diagram showing a state in which a sample collection device according to an embodiment of the present invention is installed at a sampling site.
  • FIG. 3 is a schematic diagram showing the internal structure of the sample collection device.
  • FIG. 3 is a block diagram showing a control section of the sample collecting device and parts controlled by the control section.
  • FIG. 3 is a schematic diagram showing a configuration near a water sampling port and a drain port of the sample collection device.
  • 1 is a flowchart showing each process from sample collection to inspection of the sample in this embodiment.
  • FIG. 3 is a first schematic diagram showing the operation of the sampling section in this embodiment.
  • FIG. 6 is a second schematic diagram showing the operation of the collection unit in this embodiment.
  • FIG. 7 is a third schematic diagram showing the operation of the sampling section in this embodiment.
  • FIG. 7 is a diagram showing the configuration of a sample collecting section in a sample collecting device according to a second embodiment of the present invention, and is a schematic diagram showing a state in which sewage is absorbed into an absorber.
  • FIG. 7 is a schematic diagram showing a state in which a sample squeezed from an absorber is being collected in a sample collection device according to a second embodiment.
  • FIG. 7 is a diagram showing the configuration of a sample collecting section in a sample collecting device according to a third embodiment of the present invention, and is a schematic diagram showing a state in which sewage is absorbed into an absorber.
  • FIG. 7 is a diagram showing the configuration of a sample collecting section in a sample collecting device according to a third embodiment of the present invention, and is a schematic diagram showing a state in which sewage is absorbed into an absorber.
  • FIG. 7 is an exploded perspective view showing the structure of an absorbent holding tank and an absorbent throttle part in a third embodiment.
  • FIG. 3 is a plan view of the main body of the absorbent holding tank. A sectional view taken along the line AA in FIG. 16.
  • FIG. 7 is a schematic diagram showing a state in which a sample squeezed from an absorber is being collected in a sample collection device according to a third embodiment.
  • FIG. 7 is a schematic diagram showing a state in which a sample collection section is being disinfected in a sample collection device according to a third embodiment.
  • FIG. 7 is a perspective view showing the configuration of a water collection/drainage unit in a third embodiment.
  • FIG. 1 is a diagram showing a sample collection device according to an embodiment of the present invention installed at a sampling site
  • FIG. 2 is a diagram schematically showing the internal structure of the sample collection device
  • FIG. 3 is a block diagram showing a control section of the sample collecting device and parts controlled by the control section.
  • the sample collection device includes a housing 100, a sample collection section 200, a pretreatment section 300, a refrigeration section 400, and a cleaning section 500.
  • the sample collection device further includes a control section 600 that controls the operations of each section (see FIG. 3).
  • the casing 100 is made of plastic or metal and includes a door 101 (see FIG. 1) that can be opened and closed.
  • the sample collection section 200 includes a water sampling pipe 201, a water spray head 202, a first absorbent holder 210, a second absorbent holder 220, and a first funnel section 230 disposed below the first absorbent holder 210. , a second funnel section 240 disposed below the second absorbent holder 220, a third funnel section 260 disposed below the second funnel section 240, and a third funnel section 260 disposed above the second absorbent holder 220. It includes a pressing part 270, a first liquid pump 203, an absorbent holder drive mechanism 223 (see FIG. 3), and a pressing part driving mechanism 274 (see FIG. 3).
  • the water sampling pipe 201 is made of a vinyl hose or a vinyl chloride pipe, and one end thereof is connected to the water sprinkling head 202. The other end of the water sampling pipe 201 is led out of the housing 100 through a through hole provided in the side wall of the housing 100.
  • a first liquid feeding pump 203 is disposed midway through the water sampling pipe 201 .
  • a collection cup arrangement section 250 which is a space in which a sample collection cup 290 is arranged, is provided below the second funnel section 240, and a third funnel section 260 is provided below the collection cup arrangement section 250.
  • the lower portions of the first funnel section 230 and the third funnel section 260 are connected to a first drain pipe 309, which will be described later.
  • Both the first absorbent holder 210 and the second absorbent holder 220 have a cup-like shape with an open top, and a plurality of holes 211 and 221 of a size that the absorbent body 280 cannot pass through are provided on the bottom surface. ing.
  • the upper peripheral edge of the first absorbent holder 210 and the upper peripheral edge of the second absorbent holder 220 are connected by a hinge 212, and an absorbent holder drive mechanism 223 including a motor etc. (see FIG. 3) This allows the first absorbent body holder 210 to rotate around the hinge 212.
  • the pressing unit 270 includes a plate-shaped main body 271, a plurality of needles 272 provided on the lower surface of the main body 271, and a rod 273 connected to the upper surface of the main body 271, and includes a pressing force including a motor etc.
  • the needle 272 has a diameter smaller than the holes 211 and 221 provided in the bottom of the first absorbent holder 210 and the second absorbent holder 220, either in its entirety or in a predetermined length from its tip. and is provided at a position where it can be inserted into holes 211 and 221 provided in the first absorbent body holder 210 and the second absorbent body holder.
  • a used absorbent material storage section 711 which is a container for storing the absorbent material 280 after being used in the sample collection section 200, and a container for storing the unused absorbent material 280.
  • An unused absorbent body storage section 712 which is a container, and an absorbent body transfer mechanism 713 (see FIG. 3) are provided.
  • the absorbent body transfer mechanism 713 includes, for example, a robot hand, a rotary telescoping arm, a motor for driving them, and the like, and transports the absorbent body 280 from the first absorbent body holder 210 to the used absorbent body storage section 711.
  • the absorbent body 280 is also transferred from the unused absorbent body storage section 712 to the first absorbent body holder 210.
  • the preprocessing section 300 includes a collection cup moving mechanism 301 (see FIG. 3), a first cartridge holding section 302, a drain bottle 304, a suction pump 307, a liquid supply nozzle 311, a water supply section 313, and a solvent supply system. section 314, liquid supply nozzle drive mechanism 316 (see FIG. 3), cartridge transfer mechanism 310 (see FIG. 3), second cartridge holding section 321, piston 323, and piston drive mechanism 324 (see FIG. 3). , is equipped with.
  • the collection cup moving mechanism 301 includes, for example, a robot hand, a rotary telescoping arm, a motor for driving them, and the like.
  • the first cartridge holding section 302 is a block-shaped structure having openings on the top surface and side surfaces, and a flow path is formed inside thereof that connects the openings provided on the top surface and side surfaces with each other. .
  • the tip of the solid phase cartridge 340 used for sample pretreatment is inserted into the opening provided on the top surface of the first cartridge holding section 302, so that the solid phase cartridge 340 is in a state with the tip facing down. The cartridge is held in an upright position on the first cartridge holding section 302.
  • one end of a liquid suction pipe 303 is connected to an opening provided on the side surface of the first cartridge holding section 302 .
  • the other end of the liquid suction pipe 303 passes through a sealing plug 305 attached to the drain bottle 304 and is disposed near the inner bottom surface of the drain bottle 304.
  • a suction pipe 306 is further inserted through the sealing plug 305 , one end of the suction pipe 306 is disposed above the internal space of the drain bottle 304 , and the other end is connected to a suction pump 307 .
  • One end of a first drain pipe 309 is connected to the bottom of the drain bottle 304, and the other end of the first drain pipe 309 is connected to the outside of the casing 100 through a through hole provided in the side wall of the casing 100. being guided.
  • a drain is provided at the connection between the drain bottle 304 and the first drain pipe 309 to switch between a state in which the liquid in the drain bottle 304 is drained through the first drain pipe 309 and a state in which the liquid is not drained.
  • a liquid solenoid valve 308 is provided.
  • the other end of the first drain pipe 309 is connected to a second drain pipe 102 and a common drain pipe 332, which will be described later, via a first switching valve 331.
  • a second liquid feeding pump 333 is attached.
  • a liquid supply nozzle 311 is arranged above the first cartridge holding part 302 , and the base end side of the liquid supply nozzle 311 is connected to a water supply part 313 and a solvent supply part 314 via a first liquid supply pipe 312 . It is connected.
  • the water supply unit 313 and the solvent supply unit 314 each include a tank for storing liquid, and a liquid supply mechanism including a syringe pump or the like for delivering a specified amount of liquid from the tank to the first liquid supply pipe 312. .
  • water for example, pure water or distilled water
  • water for example, pure water or distilled water
  • water for example, pure water or distilled water
  • water that has been adsorbed on the solid phase 341 (for example, polymer) in the solid phase cartridge 340 is stored in the tank of the water supply unit 313.
  • An elution solvent for eluting the detection target is stored.
  • a second switching valve 315 is provided on the first liquid supply pipe 312 to selectively connect either the water supply section 313 or the solvent supply section 314 to the liquid supply nozzle 311.
  • a second cartridge holder 321 is provided near the first cartridge holder 302 and is capable of holding the solid phase cartridge 340 with its tip facing downward. Further, between the first cartridge holding section 302 and the second cartridge holding section 321, there is a cartridge transfer mechanism 310 (see FIG. 3) that includes a robot hand, a rotary telescoping arm, a motor for driving them, and the like. It is provided. A piston 323 is provided above the second cartridge holding portion 321, and the piston 323 is connected to a piston drive mechanism 324 (see FIG. 3) that includes a motor and the like.
  • a sample container placement portion 322, which is a space in which a sample container 350 is placed, is provided below the second cartridge holding portion 321.
  • a used cartridge storage section 714 which is a container for storing the solid phase cartridge 340 after being used in the pretreatment section 300, and an unused solid phase cartridge 340 are stored.
  • An unused cartridge storage section 715 which is a container for storing the unused cartridges, is provided.
  • the refrigerator section 400 includes a refrigerator 410, a refrigerator door opening/closing mechanism 412 (see FIG. 3) including a motor, etc. for opening and closing the refrigerator door (hereinafter referred to as refrigerator door 411), and a refrigerator door opening/closing mechanism 412 (see FIG. 3) disposed inside the refrigerator 410.
  • a sample container holder 420, a holder rotation mechanism 423 (see FIG. 3), and a sample container transfer mechanism 430 (see FIG. 3) are provided.
  • the sample container holder 420 is a cylindrical structure having a plurality of recesses 421 on its upper surface capable of accommodating the sample containers 350, and is rotated around a central axis extending vertically by a holder rotation mechanism 423 having a motor or the like.
  • the sample container transfer mechanism 430 is a mechanism for transferring the sample container 350 from the sample container placement section 322 to the recess 421 of the sample container holder 420, and includes, for example, a robot hand, a rotary telescoping arm, and a mechanism for driving them. Equipped with a motor, etc.
  • the cleaning unit 500 includes a cleaning nozzle 501 attached to the ceiling surface of the housing 100, a cleaning nozzle rotation mechanism 502, a disinfectant supply unit 503 for supplying a disinfectant or a rinse liquid to the cleaning nozzle 501, and a rinse liquid. It includes a supply section 504 and a second drain pipe 102 for discharging the disinfectant and rinsing liquid sprayed into the housing 100 to the outside.
  • the cleaning nozzle 501 is configured to rotate around a central axis extending vertically by a cleaning nozzle rotating mechanism 502 equipped with a motor or the like while jetting liquid in multiple directions from right side to right below.
  • the cleaning nozzle 501 is connected to a disinfectant liquid supply section 503 and a rinse liquid supply section 504 via a second liquid supply pipe 505.
  • the disinfectant solution supply section 503 and the rinsing solution supply section 504 each include a tank that stores liquid and a liquid supply mechanism that includes a pump or the like for sending the liquid in the tank to the second liquid supply pipe 505.
  • a third switching valve 506 is provided on the second liquid supply pipe 505 to selectively connect either the disinfecting liquid supply section 503 or the rinsing liquid supply section 504 to the cleaning nozzle 501.
  • the ends of the water sampling pipe 201 and the common drain pipe 332 guided outside the housing 100 are inserted into a sewage facility 900 provided at a predetermined sampling site.
  • the sampling location may be, for example, within the premises of the facility to be inspected or in its vicinity.
  • Facilities to be inspected may be facilities such as nursing care facilities, hospitals, schools, companies, etc., where the main residents (employees, residents, users, etc.) can be identified, on or near their premises. , but is not limited to this, and may be, for example, a commercial facility or an apartment complex.
  • the sewage equipment 900 into which the ends of the water sampling pipe 201 and the common drain pipe 332 are inserted has access to the sewage from the ground on the route for the sewage discharged from the facility to flow into the public sewage pipe.
  • Equipment such as sewage basins can be suitably used.
  • a predetermined area may be the subject of inspection.
  • the predetermined collection site is a point where sewage discharged from a predetermined area is collected. Examples of such collection sites include manholes and sewage treatment plants in the city.
  • the water sampling pipe 201 and the common drain pipe 332 are fixed along a single rod 801, and the tip of the water sampling pipe 201 (i.e., the water sampling port) is located near the lower end of the rod 801. ) and the tip of the common drain pipe 332 (that is, the drain port) are bent in a direction perpendicular to the rod 801 and facing in opposite directions.
  • a metal pipe can be suitably used as the rod 801, but the present invention is not limited to this, and a plastic or wooden rod can also be used.
  • the water sampling pipe 201 and the common drain pipe 332 are arranged such that the water sampling port faces the upstream side of the flow of sewage and the drain port faces the downstream side of the flow of sewage.
  • a hollow water sampling cup 802 in which a large number of small holes 803 with a diameter smaller than the water sampling port are formed is attached to the end of the water sampling pipe 201.
  • a metal lattice 804 is erected so as to be perpendicular to the plane on which 803 is formed.
  • a grid 804 for example, a commercially available wire net (also called a mesh panel) can be used.
  • a lid 910 made of plastic or metal is attached to the sewage equipment 900 into which the water sampling pipe 201 and the common drain pipe 332 are inserted.
  • the lid 910 has a through hole 911 through which the water sampling tube 201, the common drain tube 332, and the rod 801 are inserted.
  • the housing 100 is placed on the ground near the sewage facility 900. Between the sewage equipment 900 and the casing 100, a water sampling pipe 201 and a common drain pipe 332 are arranged so as to run along the ground surface, and are protected by a commercially available cable protector 912 made of rubber or the like. 332 is coated.
  • the control unit 600 controls the operations of the above-mentioned units, and is configured by a computer such as a microcomputer housed in the housing 100, for example.
  • a computer such as a microcomputer housed in the housing 100, for example.
  • the refrigerator 410, the drain electromagnetic valve 308, the first switching valve 331, the second switching valve 315, and the third switching valve 506 are also controlled by the control unit 600.
  • an installation person installs the sample collection device according to this embodiment at a sampling site, and then inserts the tips of the water sampling pipe 201 and the common drain pipe 332 (i.e., the water sampling port and the drain port) into the sewage equipment 900. Then, by turning on a power switch (not shown) attached to the control unit 600, the control unit 600 is activated.
  • Step 1 Pumping up sewage and supplying it to the absorber
  • the computer constituting the control unit 600 stores information such as the number of sample collection times, the sample collection period for each time (for example, the time of day during which the sample is collected), and the flow rate at the time of sample collection (i.e., the first liquid pump 203). flow rate) etc. are set and stored in advance by the person in charge of installation, etc., and when the timing to start the first sample collection arrives after the control unit 600 is activated, the first sample collection is started under the control of the control unit 600.
  • the liquid feed pump 203 is activated. As a result, sewage is drawn up into the water sampling pipe 201 and sprayed in a shower form from the water spray head 202 (see the left side of FIG.
  • a portion of the sewage sprayed from the sprinkler head 202 is absorbed by the absorber 280 housed in the first absorber holder 210 .
  • absorbent cotton can be suitably used as the absorbent body 280, but a sponge, sponge, or the like can also be used instead.
  • the remaining part of the sewage sprayed from the sprinkler head 202 passes through the absorber 280 and falls into the first funnel part 230 through the hole 211 formed in the bottom surface of the first absorber holder 210. Further, some of the solid content such as feces contained in the sewage cannot enter into the absorber 280 and remains attached to the upper surface of the absorber 280.
  • control unit 600 starts the second liquid feeding pump 333 simultaneously with starting the first liquid feeding pump 203 (or after a predetermined period of time has elapsed thereafter).
  • the first drain pipe 309 and the common drain pipe 332 are connected by the first switching valve 331, and the sewage that has fallen into the first funnel part 230 is transferred to the first drain pipe. 309 , the first switching valve 331 , and the common drain pipe 332 to return to the sewage facility 900 .
  • Step 2 Inversion of absorber and sample collection
  • a predetermined sampling period for example, several hours to several days
  • the control unit 600 stops the first liquid feeding pump 203 and absorbs the absorbent material.
  • the first absorbent body holder 210 is rotated around the hinge 212 by the holder drive mechanism 223 (see the right side of FIG. 6).
  • the first absorbent holder 210 is turned upside down and placed over the second absorbent holder 220.
  • the pressing section drive mechanism 274 lowers the pressing section 270.
  • the needle 272 provided in the pressing part 270 comes into contact with the absorbent body 280 in the first absorbent body holder 210 through the hole 211 provided in the bottom surface of the first absorbent body holder 210, and thereby the absorbent body 280 is pushed downward and falls into the second absorbent body holder 220 (see the right side of FIG. 7).
  • the absorbent body 280 is turned upside down, and the solid content described above is attached to the lower surface of the absorbent body 280.
  • the pressing part 270 is retracted upward by the pressing part drive mechanism 274, and the first absorbent holder 210 is rotated around the hinge 212, thereby returning the first absorbent holder 210 to its original position (first (see left side of FIG. 8).
  • the pressing part 270 is lowered again, and the body part 271 of the pressing part 270 presses the absorbent body 280 from above, thereby squeezing out the sewage absorbed in the absorbent body 280 (see the right side of FIG. 8).
  • the squeezed sewage passes through the hole 221 formed on the bottom surface of the second absorbent holder 220 and falls into the second funnel part 240 together with the solid content attached to the bottom surface of the absorbent body 280.
  • a sample collection cup 290 that can accommodate a specified amount of liquid is arranged in the collection cup arrangement part 250 located below the second funnel part 240, and the sewage and solids that have fallen into the second funnel part 240 are The sample collection cup 290 accommodates the sample. Further, at this time, the liquid overflowing from the sample collection cup 290 falls into the third funnel part 260 and is returned to the sewage facility 900 via the first drain pipe 309.
  • Step 3 Sample pretreatment
  • a cartridge solid phase cartridge 340 filled with a solid phase 341 for adsorbing a detection target (ie, a pathogen or chemical substance in sewage) is used.
  • a detection target ie, a pathogen or chemical substance in sewage
  • a solid phase made of a polymer having a hydrophobic group is used as the solid phase 341.
  • an HLB solid phase hydrophilic lipophilic balanced solid phase
  • an appropriate solid phase can be used depending on the substance to be detected. be able to.
  • the solid phase 341 is activated by flowing water through the solid phase cartridge 340 in advance.
  • the control unit 600 connects the liquid supply nozzle 311 and the water supply unit 313 using the second switching valve 315, and connects the liquid supply nozzle 311 to the first cartridge using the liquid supply nozzle drive mechanism 316. It is moved above the solid phase cartridge 340 held on the holding part 302. In this state, the control unit 600 controls the liquid supply mechanism of the water supply unit 313 to send water from the water supply unit 313 to the first liquid supply pipe 312. As a result, a predetermined amount of water is supplied into the solid phase cartridge 340.
  • the intake pump 307 is activated by the control unit 600, whereby the air inside the drain bottle 304 is discharged to the outside through the intake pipe 306, and the inside of the drain bottle 304 is brought into a reduced pressure state.
  • the water in the solid phase cartridge 340 is sucked into the drain bottle 304 via the flow path in the first cartridge holding section 302 and the liquid suction pipe 303.
  • control section 600 controls the collection cup moving mechanism 301 to move the sample collection cup 290 placed in the collection cup placement section 250 of the sample collection section 200 into the solid phase cartridge held in the first cartridge holding section 302. 340 and further tilt the sample collection cup 290 to pour the sample in the sample collection cup 290 into the solid phase cartridge 340 (see the left side of FIG. 9). Thereafter, the sample in the solid phase cartridge 340 is sucked by driving the suction pump 307 again (see the right side of FIG. 9). As a result, the sample passes through the solid phase 341, and in the process, the object to be detected in the sample is adsorbed to the solid phase 341.
  • the impurities in the sample flow out from the solid phase cartridge 340 without being adsorbed by the solid phase 341 and are stored in the drain bottle 304.
  • Contaminants are powders or fine particles, or chemicals or viruses other than the detection target, and such contaminants inhibit the detection of the detection target in subsequent tests (e.g., virus detection by PCR, etc.) there is a possibility.
  • the sample collection device in the process of adsorbing the detection target to the solid phase 341 (and the process of eluting the detection target from the solid phase 341, which will be described later), it is possible to separate impurities from the detection target. Therefore, it is expected that the detection accuracy of the final object to be detected will be improved.
  • the control unit 600 controls the second switching valve 315 to connect the liquid supply nozzle 311 and the solvent supply unit 314, and then causes the solvent supply unit 314 to send out a predetermined amount of the elution solvent.
  • the elution solvent is discharged from the liquid supply nozzle 311 into the solid phase cartridge 340 (see the left side of FIG. 10).
  • the control section 600 controls the cartridge transfer mechanism 310 to move the solid phase cartridge 340 from the first cartridge holding section 302 to the second cartridge holding section 321.
  • the piston 323 is lowered by the piston drive mechanism 324 to pressurize the inside of the solid phase cartridge 340 (see the right side of FIG. 10).
  • the elution solvent in the solid phase cartridge 340 passes through the solid phase 341, and in the process, the detection target substance adsorbed on the solid phase 341 is eluted from the solid phase cartridge 340.
  • the eluate from the solid phase cartridge 340 (that is, the sample after pretreatment) is accommodated in the sample container 350 arranged in the sample container arrangement section 322.
  • the amount of elution solvent used in this step is smaller than the amount of the sample poured from the sample collection cup 290 into the solid phase cartridge 340 (for example, about 1/100 to 1/20 of the amount of the sample). ).
  • the sample after pretreatment contains the detection target substance at a higher concentration than the sample before pretreatment.
  • impurities that are not eluted by the elution solvent in this step remain in the solid phase cartridge 340 while being adsorbed to the solid phase 341.
  • the detection target is a virus
  • an organic solvent such as an alcohol solvent
  • isopropanol, ethanol, methanol, or the like can be suitably used. It is known that viruses are destroyed when they are reacted with an organic solvent such as alcohol.
  • the present inventor has found that even when the virus adsorbed to the solid phase 341 is eluted with an organic solvent, the virus nucleic acid (DNA or RNA) necessary for virus detection by PCR etc., which will be described later, is It was discovered that it is possible to elute up to 100% without destroying the material.
  • viruses to be detected in this embodiment include, but are not limited to, coronaviruses (e.g., novel coronavirus (SARS-CoV-2)) and enveloped viruses such as influenza viruses.
  • coronaviruses e.g., novel coronavirus (SARS-CoV-2)
  • enveloped viruses such as influenza viruses.
  • a non-enveloped virus such as norovirus or poliovirus may be used as the detection target.
  • the same type of liquid i.e., water supplied from the water supply unit 313
  • water supplied from the water supply unit 313 is used in both the step of activating the solid phase 341 and the step of washing away impurities from the solid phase 341.
  • Different liquids may be used in both steps.
  • a liquid is used that elutes the impurities but does not elute the object to be detected.
  • a solvent supplied from the solvent supply section 314 may be made to flow into the solid phase cartridge 340.
  • the liquid contained in the drain bottle 304 is transferred to the first liquid by the control unit 600 opening the drain electromagnetic valve 308 at a predetermined timing and operating the second liquid sending pump 333. It is returned to the sewage facility 900 via the drain pipe 309 and the common drain pipe 332.
  • the control unit 600 controls the refrigerator door opening/closing mechanism 412 to open the refrigerator door 411, and also controls the holder rotation mechanism 423 to open the plurality of holes provided on the sample container holder 420.
  • the sample container holder 420 is rotated so that one of the recesses 421 (the one in which the sample container 350 is not held) comes to a standby position near the refrigerator door 411.
  • the control section 600 further controls the sample container transfer mechanism 430 to move the sample container 350 from the sample container placement section 322 above the standby position, and then lowers the sample container 350, thereby moving the sample container 350. It is housed in the recess 421.
  • the refrigerator door 411 is closed by the refrigerator door opening/closing mechanism 412. Since the inside of the refrigerator 410 is maintained at a lower temperature (for example, 4° C.) than the outside air, by storing the sample container 350 inside the refrigerator 410, detection targets (such as viruses or bacteria) in the sample due to high temperature can be removed. can prevent degeneration and deterioration.
  • detection targets such as viruses or bacteria
  • Step 5 Removal of used absorber and cartridge
  • the absorbent body 280 in the first absorbent body holder 210 is transferred to the used absorbent body storage section 711 by the absorbent body transfer mechanism 713, and further, the absorbent body 280 in the first absorbent body holder 210 is transferred to the used absorbent body storage section 711 by the cartridge transfer mechanism 310.
  • Solid phase cartridge 340 is transferred to used cartridge storage section 714.
  • Step 6 Cleaning inside the housing
  • the inside of the casing 100 is cleaned by the cleaning section 500.
  • the cleaning nozzle 501 and the disinfectant supply section 503 are connected by the third switching valve 506, and the disinfectant solution is stored in the tank of the disinfectant supply section 503 by the supply mechanism provided in the disinfectant supply section 503.
  • a disinfectant solution (for example, hypochlorous acid water) is supplied to the second liquid supply pipe 505.
  • disinfectant is sprayed from the cleaning nozzle 501.
  • the cleaning nozzle rotation mechanism 502 the disinfectant can be evenly sprayed into the housing 100.
  • the inner bottom surface of the casing 100 has an inclined shape, and the disinfectant liquid that is injected into the casing 100 and reaches the inner bottom surface flows into the second drain pipe 102 along the slope.
  • the disinfectant liquid that has flowed into the second drain pipe 102 is drawn into the common drain pipe 332 by the action of the second liquid pump 333 and is discharged to the sewage facility 900.
  • the third switching valve 506 is switched under the control of the control unit 600, and the rinsing liquid supply unit 504 is connected to the cleaning nozzle 501. Furthermore, by operating the liquid supply mechanism provided in the rinse liquid supply unit 504 under the control of the control unit 600, the rinse liquid stored in the tank of the rinse liquid supply unit 504 (e.g. tap water) is sprayed from the cleaning nozzle 501 over a predetermined period of time. The rinsing liquid injected into the housing 100 at this time also flows into the second drain pipe 102 like the disinfectant, and is discharged to the sewage facility 900 via the common drain pipe 332.
  • the rinse liquid stored in the tank of the rinse liquid supply unit 504 e.g. tap water
  • the disinfectant and rinsing liquid that have flowed into the second drain pipe 102 are discharged to the sewage facility 900 via the common drain pipe 332, but instead A drain tank may be provided, and the disinfectant and disinfectant liquid discharged from the second drain pipe 102 may be stored in the drain tank.
  • Step 7 Set new absorber and cartridge
  • the unused absorbent body 280 in the unused absorbent body storage section 712 is transferred and set in the first absorbent body holder 210 by the absorbent body transfer mechanism 713, and then , the unused solid phase cartridge 340 in the unused cartridge storage section 715 is transferred and set on the first cartridge holding section 302 by the cartridge transfer mechanism 310 .
  • Step 8 Determine whether all sample collection has been completed. Subsequently, the control unit 600 determines whether or not sample collection has been completed a preset number of times. If it is determined in step 8 that the preset number of sample collections has been completed, the process proceeds to step 10, which will be described later.
  • Step 9 Wait until the next collection timing
  • the process waits until the next sample collection start timing. Thereafter, the process returns to step 1, and steps 1 to 9 are repeatedly executed until it is determined in step 8 that the preset number of sample collections have been completed.
  • Step 10 Collection and shipping of sample containers
  • the person in charge of collection for example, a staff member of the facility to be inspected
  • the refrigerator door 411 at a predetermined timing.
  • the collected sample containers are shipped to a predetermined testing institution using, for example, a refrigerated delivery service provided by a general delivery company.
  • the refrigerated home delivery service is a service that delivers packages using vehicles and equipment equipped with a refrigerated function.
  • Step 11 Inspecting the sample
  • the sample container 350 delivered to the predetermined testing agency is opened by a person in charge of testing at the testing agency, and the sample inside the sample container 350 is subjected to a predetermined test.
  • the object to be tested is bacteria, fungi, or DNA viruses
  • the sample is subjected to DNA extraction processing, and then primers specific to the bacteria, fungi, or virus to be tested are used.
  • DNA is amplified and detected by polymerase chain reaction (PCR).
  • PCR polymerase chain reaction
  • the object to be tested is an RNA virus
  • the sample is subjected to RNA extraction processing and then subjected to reverse transcription PCR (RT-) using primers specific to the virus to be detected.
  • DNA complementary DNA: cDNA
  • RNA complementary DNA
  • PCR complementary DNA
  • Real-time PCR is a method for measuring DNA concentration by detecting in real time a fluorescent signal generated by the interaction between an amplified DNA fragment and a reagent, and it is a method for measuring the amplification product by PCR or RT-PCR in a timely manner. Can be done.
  • mass spectrometry is performed as the predetermined test, and based on the mass spectrum obtained by the mass spectrometry, it is determined whether the test object exists in the sample. will be judged.
  • sample collection, pretreatment, and refrigeration are automatically performed at the collection site. Therefore, there is no need for a specialist to regularly visit the sampling site and collect samples, and the effort and cost associated with sample collection can be reduced. Furthermore, since the sample can be concentrated by the pretreatment, the volume of the sample to be transported to the testing institution can be reduced, thereby reducing transportation costs. Furthermore, according to the sample collection device according to the present embodiment, the absorber 280 and the solid phase cartridge 340 are automatically replaced each time sample collection and pretreatment are completed, reducing the effort involved in replacing them. At the same time, it is possible to avoid the risk of infection of the worker due to touching the used absorber 280 and solid phase cartridge 340.
  • the absorbent body 280 Furthermore, by inverting the absorbent body 280 and collecting the sample as described above, the sewage that has permeated into the absorbent body 280 and the solid matter such as feces that has adhered to the absorbent body 280 can be collected together. be able to.
  • a plurality of needles 272 are provided on the lower surface of the pressing part 270 for pressurizing the absorber 280, but such needles 272 do not necessarily need to be provided. Further, the pressing part 270 is not provided, and by flowing a predetermined eluate (for example, an alkaline eluent) to the absorber 280, the sample held in the absorber 280 is eluted and collected in the sample collection cup 290. Good too.
  • a predetermined eluate for example, an alkaline eluent
  • the absorber 280 in addition to the above-mentioned absorbent cotton, sponge, or sponge, a water-absorbing polymer or the like can also be used.
  • the first absorbent body holder 210 and the second absorbent body holder 220 are connected to each other by the hinge 212, but the sample collection unit 200 in this embodiment turns the absorbent body 280 upside down.
  • the structure may be any structure as long as the sample held in the absorber 280 can be squeezed out (or eluted) after the sample is absorbed, and the hinge 212 as described above does not necessarily need to be provided.
  • the sample collection device may not perform the above-described reversal of the absorber 280.
  • the second absorbent holder 220, the second funnel part 240, and the absorbent holder drive mechanism 223 are not provided, but the collection cup placement part 250 and the third funnel part 260 are provided below the first funnel part 230. shall be.
  • the pumped sewage may be allowed to flow into the solid phase cartridge 340 without being absorbed by the absorber 280.
  • the sewage pumped up through the water sampling pipe 201 may be made to flow directly into the solid phase cartridge 340, or the pumped sewage water may be collected into the sample collection cup 290 and then placed in the sample collection cup 290. Sewage may be poured from 290 into the solid phase cartridge 340.
  • the sample is concentrated by solid phase extraction in the pretreatment section 300, but instead of this, components for inactivating pathogens are added to the sample (without performing solid phase extraction). It may also be added to.
  • the pretreatment unit 300 transfers the sample collected in the sample collection cup 290 to a sample container 350 (one with a larger capacity than the sample collection cup 290), and then inactivates the pathogen in the sample container 350. It shall be equipped with a mechanism for adding ingredients.
  • a component for inactivating pathogens for example, an organic solvent can be used, and in particular, an alcohol solvent such as isopropanol, ethanol, or methanol can be suitably used.
  • the pretreatment unit 300 uses the sample in a medium for growing the bacterium or fungus to be detected, instead of solid-phase extraction as described above. may be added.
  • the preprocessing section 300 is, for example, equipped with a mechanism for collecting a predetermined amount of sample from the sample collection cup 290 and adding it to the sample container 350 in which the medium is previously stored.
  • the sample and culture medium in the sample container 350 correspond to the pretreated sample in the present invention.
  • the absorber and the solid phase cartridge are replaced automatically, but the replacement work may be performed manually by a user (for example, an employee of the facility to be inspected).
  • a user for example, an employee of the facility to be inspected.
  • only the removal of the used absorbent body 280 and the solid phase cartridge 340 is performed automatically on the apparatus side, and a new absorbent body and the solid phase cartridge 340 are automatically removed.
  • the solid phase cartridge may be attached manually by the user.
  • the absorbent body 280 that has absorbed sewage is pressed from above by the pressing part 270 to squeeze out the sewage from the absorbent body 280.
  • a configuration may be adopted in which the absorber 280 is squeezed by two rollers to squeeze out the sewage.
  • An embodiment (hereinafter referred to as a second embodiment) of a sample collection device according to the present invention having such a configuration will be described with reference to FIGS. 11 and 12.
  • 11 and 12 are diagrams showing the configuration of a sample collection section (corresponding to the sample collection section 200 in the first embodiment) in the sample collection device according to the present embodiment.
  • the configuration other than the sample collection section is the same as that of the first embodiment, so the description thereof will be omitted here.
  • components that are the same as or correspond to those shown in FIGS. 1 to 10 are designated by the same reference numerals having the same last three digits, and the description thereof will be omitted as appropriate.
  • the sample collection section in this embodiment includes an absorber holding tank 1901 in which the absorber 1280 is accommodated, and an absorber constricting section 1902 (corresponding to the absorber pressurizing section in the present invention) provided at the upper part of the absorber holding tank 1901. ) and a used absorbent body receiving part 1903 in which the absorbent body 1280 squeezed by the absorbent body constricting part 1902 is accommodated. Furthermore, in the sample collection section of this embodiment, the end (outlet end) of the water sampling tube 1201 is placed directly above the absorber holding tank 1901. Note that in FIG. 12, illustration of the water sampling pipe 1201 is omitted for convenience of drawing.
  • the absorbent holding tank 1901 has a generally rectangular shape when viewed from above, and a rotating shaft 1904 parallel to the side is attached to one side of the tank. Further, a holding tank driving mechanism 1905 (not shown in FIG. 11) is attached to the side opposite to the one side (hereinafter referred to as the opposite side), and the holding tank driving mechanism 1905 lifts the opposite side. This allows the absorbent holding tank 1901 to be rotated around the rotation axis 1904 and tilted. In this embodiment, as shown in FIG. 12, a cable 1906 is attached to the opposite side, and a motor (not shown) provided in the holding tank drive mechanism 1905 winds up the cable 1906, thereby driving the absorber holding tank.
  • the holding tank drive mechanism 1905 is configured to rotate the holding tank 1901, the structure of the holding tank drive mechanism 1905 is not limited to this, for example, it may be configured to push up the opposite side of the absorbent holding tank 1901 from below.
  • a drain port 1907 which is a circular or polygonal opening, is formed on the bottom surface of the absorbent holding tank 1901 near the one side (the side near the rotation axis 1904). It communicates with a tubular holding tank drain pipe 1908 connected to the lower part of the holding tank 1901.
  • the sample collection unit in this embodiment further includes a drain valve 1909 for opening and closing this drain port 1907.
  • the drain valve 1909 includes a cylindrical portion 1910 whose cross section has substantially the same shape and dimensions as the drain port 1907, and a cylindrical (or polygonal column) surplus water inflow portion 1911 connected to the upper end of the cylindrical portion 1910. It is equipped with.
  • the surplus water inflow section 1911 has a hollow structure.
  • the outer diameter of the surplus water inlet 1911 (if the surplus water inlet 1911 is polygonal, the diameter of its circumscribed circle) is preferably larger than the diameter of the cylindrical part 1910, but is not limited to this. do not have.
  • the internal space of the surplus water inflow part 1911 communicates with the inside of the cylindrical part 1910, and surplus water inflow ports 1912, which are a plurality of openings communicating with the internal space, are formed on the circumferential surface of the surplus water inflow part 1911. ing.
  • This drain valve 1909 is movable in the vertical direction by a drain valve drive mechanism 1913 that includes a motor, a rotation-to-linear conversion mechanism, and the like.
  • the drain port 1907 When the drain valve 1909 is lowered and the cylindrical portion 1910 is inserted into the drain port 1907, the drain port 1907 is closed, and when the drain valve 1909 is raised and the cylindrical portion 1910 is pulled out from the drain port 1907, the drain port 1907 is opened. be done.
  • An L-shaped pipe 1914 is arranged below the absorbent holding tank 1901 with one end facing upward and the other end facing sideways.
  • the one end of the L-shaped pipe 1914 is connected to the lower end of the holding tank drain pipe 1908 via a flexible pipe 1915, and the other end of the L-shaped pipe 1914 is connected to the side via a rotary joint 1916.
  • It is connected to one end of a side extension pipe 1917, which is a straight pipe extending in the direction.
  • a first outlet pipe 1918 extending in a direction substantially perpendicular to the extending direction of the lateral extending pipe 1917 is connected to the other end of the lateral extending pipe 1917.
  • a second outlet pipe 1919 extending parallel to and in the opposite direction to the outlet pipe 1918 is connected.
  • a funnel portion 1920 is provided below the side extension pipe 1917 and directly below the position where the first outlet pipe 1918 is connected.
  • the lower part of the funnel part 1920 is connected to a drain pipe 1309 (first drain pipe 309 in the first embodiment) extending outside the casing (corresponding to the casing 100 in the first embodiment) of the sample collection device according to the present embodiment. (equivalent to).
  • a collection container arrangement section 1250 is provided below the side extension pipe 1917 and directly below the position where the second outlet pipe 1919 is connected, which is a space in which a collection container 1290 is arranged.
  • a drain pipe driving mechanism 1921 equipped with a motor etc.
  • the rotary joint 1916, the lateral extension pipe 1917, the first outlet pipe 1918, the second outlet pipe 1919, and the drain pipe drive mechanism 1921 correspond to the flow path switching section in the present invention.
  • the absorbent body squeezing unit 1902 includes two rollers 1922 having a length greater than the width of the absorbent body 1280, and a roller drive mechanism 1923 (not shown in FIG. 12) for rotating these rollers 1922. ing.
  • the two rollers 1922 are arranged parallel to each other with a gap of about 0.1 mm to several millimeters (for example, 0.3 mm to 2 mm, preferably 0.5 mm to 1.5 mm). By rotating each roller 1922 in opposite directions with one end of the absorbent body 1280 sandwiched between them, the absorbent body 1280 from one end to the other end can be sequentially fed between both rollers 1922 and compressed.
  • the holding tank drive mechanism 1905, drain valve drive mechanism 1913, drain pipe drive mechanism 1921, and roller drive mechanism 1923 described above are all controlled by a control unit (corresponding to the control unit 600 of the first embodiment) not shown.
  • the absorber holding tank 1901 when absorbing sewage into the absorber 1280, the absorber holding tank 1901 is held horizontally, the drain port 1907 is closed by the drain valve 1909, and the absorber 1280 is Set it in the holding tank 1901. At this time, one end of the absorber 1280 is held between two rollers 1922 of the absorber squeezing section 1902. Further, the lateral extension pipe 1917 is in the above-mentioned drain position. In this state, when sewage is pumped up from the sewage equipment by the first liquid pump 1203 and discharged from the end of the water sampling pipe 1201, the sewage accumulates in the absorber holding tank 1901.
  • sewage that exceeds a predetermined water level flows into the surplus water inlet 1911 from the surplus water inlet 1912 provided in the drain valve 1909, passes through the cylindrical part 1910, and is discharged from the absorber holding tank 1901. Therefore, the sewage in the absorber holding tank 1901 does not exceed the predetermined water level.
  • the sewage discharged from the absorber holding tank 1901 at this time passes through the holding tank drain pipe 1908, flexible pipe 1915, L-shaped pipe 1914, side extension pipe 1917, and first outlet pipe 1918 to the funnel part. 1920 and is returned to the sewage facility through a drain pipe 1309 and a common drain pipe (not shown; corresponds to the common drain pipe 332 in the first embodiment).
  • the first liquid feeding pump 1203 is stopped to end the supply of sewage to the absorber holding tank 1901.
  • the drain valve 1909 is raised to open the drain port 1907, and the absorbent holding tank 1901 is tilted to drain the sewage accumulated in the absorbent holding tank 1901.
  • the lateral stretching pipe 1917 is brought to the above-mentioned collection position, and the roller 1922 of the absorbent body squeezing part 1902 is rotated.
  • the absorbent body 1280 is sent from the absorbent body holding tank 1901 to the used absorbent body receiving section 1903, and the sewage that has been absorbed in the absorbent body 1280 is squeezed out into the absorbent body holding tank 1901.
  • the sewage squeezed out into the absorber holding tank 1901 passes through a drain port 1907, a holding tank drain pipe 1908, a flexible pipe 1915, an L-shaped pipe 1914, a side extension pipe 1917, and a second outlet pipe 1919. It is accommodated in a collection container 1290.
  • the sewage (i.e., sample) contained in the collection container 1290 undergoes a predetermined pretreatment in a pretreatment section (not shown; corresponds to the pretreatment section 300 in the first embodiment), and then is sent to a refrigeration section (not shown). (corresponding to the refrigeration unit 400 of the first embodiment) and then subjected to inspection.
  • a pretreatment section not shown; corresponds to the pretreatment section 300 in the first embodiment
  • a refrigeration section not shown
  • the end of the water sampling pipe 1201 is arranged above the absorbent body holding tank 1901, and the sewage is discharged from above the absorbent body holding tank 1901 to be supplied to the absorbent body 1280.
  • the end of the water sampling pipe 1201 may be connected to the bottom or lower side of the absorber holding tank 1901, and sewage may be supplied into the absorber holding tank 1901 from the lower part of the absorber holding tank 1901.
  • the sample collection section does not necessarily have to include such a drain valve 1909.
  • an embodiment referred to as a third embodiment having such a configuration will be described with reference to FIGS. 13 to 19.
  • FIGS. 13, 17, and 18 are schematic diagrams showing the configuration of a sample collection section (corresponding to the sample collection section 200 in Embodiment 1) in the sample collection device according to the present embodiment.
  • FIG. 14 is an exploded perspective view showing the structure of an absorbent holding tank 2901 and an absorbent squeezing section 2902 provided in the sample collection section.
  • FIG. 15 is a view of the main body 2930 of the absorbent holding tank 2901 viewed from above
  • FIG. 16 is a sectional view taken along the line AA in FIG.
  • front, rear, left, and right will be defined with the left side in FIG. 15 as the front, and the upper side in the same figure as the right side.
  • FIG. 14 is an exploded perspective view showing the structure of an absorbent holding tank 2901 and an absorbent squeezing section 2902 provided in the sample collection section.
  • FIG. 15 is a view of the main body 2930 of the absorbent holding tank 2901 viewed from above
  • FIG. 16 is a sectional view taken along the line
  • FIGS. 13 to 19 is a schematic diagram showing the configuration of a water sampling/draining unit 2931 (described later) in the same embodiment. Note that in this embodiment, the configuration other than the sample collection section and the water sampling/drainage unit 2931 is the same as that of the first embodiment, so illustration and description thereof will be omitted here.
  • constituent elements that are the same as or correspond to those shown in FIGS. 1 to 12 are designated by the same reference numerals having the same last three digits, and the description thereof will be omitted as appropriate.
  • the sample collection section in this embodiment includes an absorber holding tank 2901 in which an absorber 2280 is accommodated, and an absorber aperture section 2902 (in the present invention) provided at the upper part of the absorber holding tank 2901. (corresponding to an absorber pressing section) and a used absorbent body receiving section 2903 in which the absorbent body 2280 squeezed by the absorbent body squeezing section 2902 is accommodated.
  • the absorber squeezing part 1902 in the second embodiment is arranged on the side of the absorber holding tank 1901 (right side in FIG. 11) while squeezing the absorber 1280 pulled up from the absorber holding tank 1901.
  • the absorbent squeezing unit 2902 in this embodiment sends the absorbent body 2280 pulled up from the absorbent body holding tank 2901 to the upper part of the absorbent body holding tank 2901 while compressing it.
  • the used absorbent body receiving portion 2903 is configured to send it out.
  • the upper opening of the absorbent body holding tank 2901 is covered with a lid 2932 that partially covers the top opening, and the used absorbent body receiving part 2903 is placed on top of this lid 2932 (note that 14 to 16, illustration of the lid 2932 is omitted).
  • the absorber holding tank 2901 in this embodiment has a substantially rectangular shape when viewed from above, as in the second embodiment, and is driven by a holding tank drive mechanism 2905 (see FIG. 17; omitted in FIGS. 13 and 18). It can be rotated around the rotation axis 2904 and tilted.
  • a drain port 2907 is provided at the bottom of the side surface (referred to as the first side surface 2933) of the absorber holding tank 2901 near the rotating shaft 2904, and the drain port 2907 is connected to the flexible pipe 2915, the L-shaped pipe 2914 and a lateral extension pipe 2917 via a rotary joint 2916.
  • the configuration of the lateral extension pipe 2917 is the same as that in the second embodiment, and by rotating the lateral extension pipe 2917 around its axis by the drain pipe drive mechanism 2921, the sewage flowing out from the absorber holding tank 2901 is A state where liquid is discharged from the first outlet pipe 2918 to the funnel part 2920 (the above-mentioned drain position) and a state where the liquid is discharged from the second outlet pipe 2919 to the collection container 2290 arranged in the collection container arrangement part 2250 (the above-mentioned state) collection position).
  • the rotary joint 2916, the lateral extension pipe 2917, the first outlet pipe 2918, the second outlet pipe 2919, and the drain pipe drive mechanism 2921 correspond to the flow path switching unit in the present invention.
  • a pair of left and right disinfectant supply ports 2935 are provided at the upper part of the side surface (referred to as a second side surface 2934) opposite to the first side surface 2933 of the absorbent holding tank 2901.
  • the disinfectant supply port 2935 is connected to a disinfectant supply pipe 2937 via a flexible pipe 2936.
  • the disinfectant supply pipe 2937 is provided with a disinfectant supply pump 2938, and the pump 2938 sucks disinfectant (for example, hypochlorous acid water) from a predetermined tank, etc. , and the disinfectant solution supply port 2935 into the absorbent holding tank 2901 .
  • a piping 2936 and a disinfectant supply pipe 2937 are respectively connected to the pair of disinfectant supply ports 2935, but in FIGS. 13, 17, and 18, one disinfectant supply port is connected for convenience of drawing. 2935, piping 2936, disinfectant supply pipe 2937, and disinfectant supply pump 2938 are shown.
  • a sewage supply port 2941 is provided at the bottom of the remaining two side surfaces (referred to as a third side surface 2939 and a fourth side surface 2940) of the absorber holding tank 2901.
  • the sewage supply port 2941 is placed at the bottom of the absorbent holding tank 2901).
  • the sample collection device includes two water sampling pipes 2201, and each water sampling pipe 2201 is provided with a sewage liquid pump 2203 for pumping up sewage from the sewage equipment.
  • One end of each water sampling pipe 2201 is connected to a water sampling/drainage unit 2931 (described later) arranged in a sewage facility, and the other end is connected to a sewage supply port 2941 of the absorbent holding tank 2901.
  • the absorbent holding tank 2901 in this embodiment includes a main body 2930 and a perforated plate 2942, as shown in FIG.
  • a substantially U-shaped cable attachment member 2943 is fixed to the lower part of the rear side of the main body part 2930, for example, by screwing, and when the absorber holding tank 2901 is tilted by the holding tank drive mechanism 2905, The lower end of a cable 2906 used for this is fixed to a cable attachment hole 2944 provided in this cable attachment member 2943.
  • the main body portion 2930 includes a concave absorbent body storage portion 2945 provided at the center and a pair of primary water storage portions 2946 provided on the left and right sides thereof.
  • Each primary water storage portion 2946 is a slit-shaped recess extending in the front-rear direction, and the main body portion 2930 and each primary water storage portion 2946 are partitioned by a partition wall 2947 extending in the front-rear direction.
  • the height of the partition wall 2947 is lower than the height of the peripheral wall of the main body portion 2930, and a plurality of protrusions 2948 are provided on the upper end surface of the partition wall 2947 at predetermined intervals.
  • the perforated plate 2942 is a flat plate with a thickness of approximately several mm and includes a large number of circular (or polygonal) small holes 2949 and a rectangular notch 2950 through which the absorbent body 2280 passes. It is fitted into the upper opening of 2945. Note that the perforated plate 2942 is not limited to such a flat plate with a large number of small holes 2949, but may also be a net made of fibers arranged in a lattice shape (in this case, the mesh of the said net is small). (corresponds to hole 2949).
  • the size of the small hole 2949 provided in the perforated plate 2942 (if the small hole is circular, its diameter; if the small hole is polygonal, the diameter of its circumscribed circle) is, for example, 3 mm to 10 mm (more preferably 5 mm to 9 mm). ). Note that small holes 2949 having different sizes may be mixed.
  • Convex portions 2951 for supporting the perforated plate 2942 from below are provided at each of the four corners of the absorbent housing portion 2945, and when the perforated plate 2942 is fitted into the upper opening, the upper surface of the perforated plate 2942 and the partition wall 2947 It is configured so that the upper end surface of the holder is flush with the upper end surface of the holder. As shown in FIG.
  • the bottom surface of the primary water storage section 2946 is tapered toward the center in the front-rear direction, and a sewage supply port 2941 is opened on the side surface near the bottom of the primary water storage section 2946.
  • a rectangular parallelepiped block-shaped protrusion 2952 is provided on the front surface of the absorbent holding tank 2901 (that is, on the outside of the first side surface 2933), and the drain port 2907 passes through this block-shaped protrusion 2952 in the front-rear direction. It is formed like this.
  • the block-shaped protrusion 2952 is provided with a through hole 2953 extending in the left-right direction, and the above-mentioned rotation shaft 2904 is inserted through the through hole 2953.
  • an absorbent body damming portion 2954 is provided on the inner bottom surface of the absorbent body storage portion 2945 near the drain port 2907, which is a protrusion for preventing the drain port 2907 from being blocked by the absorbent body 2280.
  • a roller drive mechanism 2923 (see FIGS. 13 and 14; omitted in FIGS. 17 and 18), a drain pipe drive mechanism 2921, a holding tank drive mechanism 2905, a sewage liquid supply pump 2203, and a disinfectant supply pump 2938 is also controlled by a control unit (corresponding to the control unit 600 in the first embodiment) not shown.
  • the absorber 2280 when absorbing sewage into the absorber 2280, first, with the absorber holding tank 2901 horizontal, the absorber 2280 is placed in the absorber storage part of the absorber holding tank 2901. 2945, the perforated plate 2942 is attached to the upper opening of the absorbent body housing part 2945. At this time, one end of the absorbent body 2280 is pulled out above the perforated plate 2942 through a notch 2950 provided in the perforated plate 2942 and held between the two rollers 2922 of the absorbent body squeezing part 2902. Subsequently, as shown in FIG.
  • the sewage liquid feed pump 2203 pumps up sewage into the water sampling pipe 2201, and the sewage is poured into the left and right sides of the absorbent holding tank 2901. Sewage is supplied from the provided sewage supply port 2941 to the primary water storage section 2946.
  • the primary water storage section 2946 is formed in a tapered shape, as the water level of the sewage in the primary water storage section 2946 rises, the water surface of the sewage extends in the front-rear direction.
  • the sewage that has passed through the small holes 2949 falls onto the absorber 2280 placed below the perforated plate 2942 and is absorbed by the absorber 2280 .
  • the drain port 2907 of the absorber holding tank 2901 is always open, the sewage that cannot be completely absorbed by the absorber 2280 is immediately discharged without accumulating in the absorber accommodating part 2945. It is discharged into the funnel part 2920 through the sex pipe 2915, the L-shaped pipe 2914, the side extension pipe 2917, and the first outlet pipe 2918, and is discharged into the sewer through the drain pipe 2309 and the common drain pipe 2332 (see FIG. 19). Returned to equipment.
  • the absorbent holding tank 2901 in this embodiment stretches the water surface of sewage in the front-rear direction in the primary water storage part 2946, and then flows the sewage onto the perforated plate 2942 from the left and right sides of the perforated plate 2942. Furthermore, since the sewage is spread over almost the entire area of the perforated plate 2942 and then dropped from each small hole 2949, the sewage can be spread over a wide area of the absorbent body 2280 without storing the sewage inside the absorbent body housing part 2945. Sewage can be absorbed evenly throughout the area.
  • the sewage liquid sending pump 2203 is stopped to end the supply of sewage to the absorber holding tank 2901. Subsequently, as shown in FIG. 17, the absorbent holding tank 2901 is tilted, the lateral extension tube 2917 is placed in the above-mentioned collection position, and the roller 2922 of the absorbent squeezing section 2902 is rotated.
  • the absorbent body 2280 is drawn out from the absorbent body holding tank 2901 to the used absorbent body receiving part 2903, and the sewage that had been absorbed in the absorbent body 2280 is squeezed out into the absorbent body housing part 2945, and the drain port 2907 , a flexible pipe 2915 , an L-shaped pipe 2914 , a lateral extension pipe 2917 , and a second outlet pipe 2919 .
  • the subsequent treatment of the sewage (i.e., sample) contained in the collection container is the same as that in the second embodiment, and therefore will not be described here.
  • the sample collection device After collecting the sample in the collection container 2290, by supplying disinfectant to the absorber holding tank 2901, various piping provided in the absorber holding tank 2901 and the sample collecting section The inside of the can be disinfected. The procedure at this time will be explained with reference to FIG. 18. First, after moving the collection container 2290 from the collection container placement section 2250 to a preprocessing section (not shown) (corresponding to the preprocessing section 300 in the first embodiment), a predetermined waste liquid container 2955 is placed in the collection container placement section 2250. do.
  • the disinfectant supply pump 2938 is operated to supply disinfectant into the absorbent holding tank 2901 from the disinfectant supply port 2935.
  • the disinfectant solution flows into the primary water storage section 2946 and the absorbent body housing section 2945, thereby disinfecting the inside of the primary water storage section 2946 and the absorbent body housing section 2945.
  • the disinfectant solution that has flowed into the absorbent storage section 2945 passes through the drain port 2907, the flexible pipe 2915, the L-shaped pipe 2914, the lateral extension pipe 2917, and the second outlet pipe 2919, and then enters the waste liquid container 2955 described above. Exhaled inward.
  • the drain port 2907, the flexible pipe 2915, the L-shaped pipe 2914, the lateral extension pipe 2917, and the second outlet pipe 2919 are disinfected.
  • the disinfecting solution that has flowed into the primary water storage section 2946 is fed by the sewage liquid feeding pump 2203 in the opposite direction to the direction in which sewage is supplied (for example, the sewage liquid feeding pump 2203 is a tube pump, and the tube pump (in the opposite direction to that when supplying sewage), the water is discharged to the sewage equipment through the sewage supply port 2941 and the water sampling pipe 2201. This disinfects the inside of the sewage supply port 2941 and the water sampling pipe 2201.
  • the tips of the two water sampling pipes 2201 and the common drain pipe 2332 are connected to a water sampling/drainage unit 2931 (corresponding to the water sampling unit in the present invention) as shown in FIG. ).
  • This water sampling/drainage unit 2931 is installed by being fitted into an invert tank, which is a type of sewage equipment, that is, a sewage tank equipped with a groove (invert) having a substantially semicircular cross section on the bottom surface. It includes a hollow water sampling section 2956 and a tubular drainage section 2957.
  • the water sampling portion 2956 has a shape (typically a semi-cylindrical shape) obtained by cutting a cylinder along a plane parallel to its axis.
  • a rectangular area (hereinafter referred to as the upper surface plate 2958) that is a part of the wall surface corresponding to the plane (this is referred to as the upper surface of the water sampling section 2956) has a large number of small holes 2959 over almost the entire area.
  • the internal space 2960 of the water sampling section 2956 communicates with the outside via each small hole 2959.
  • the diameter of each small hole 2959 is preferably about the same as the inner diameter of the water sampling tube 2201 (for example, about 7 to 9 mm) or smaller.
  • the top plate 2958 (corresponding to the filter in the present invention) is removable from the remaining portion of the water sampling section 2956 (hereinafter referred to as the main body section 2965).
  • the upper plate 2958 is not limited to such a flat plate with a large number of small holes 2959, but may also be a net made of fibers arranged in a lattice shape (in this case, the mesh of the net is small). (corresponds to hole 2959).
  • one wall surface is a surface perpendicular to the axial direction of the main body section 2965 (hereinafter, this wall surface is referred to as a vertical wall 2961), and the other wall surface is a surface perpendicular to the axial direction of the main body section 2965.
  • the end surface is a surface that is sloped so that its outer surface faces upward (hereinafter, this sloped wall surface will be referred to as a slope wall 2962).
  • this sloped wall surface will be referred to as a slope wall 2962.
  • the side of the water sampling/drainage unit 2931 where the inclined wall 2962 is located is defined as the front
  • the side where the vertical wall 2961 is located is defined as the rear
  • the side where the top plate 2958 is located is defined as the upper side.
  • the angle formed by the axial direction of the main body portion 2965 and the inclined wall 2962 is not particularly limited, but may be, for example, 20° to 70°, preferably 30° to 60°.
  • the drainage section 2957 is formed integrally with the top plate 2958 of the water sampling section 2956 and extends in a direction parallel to the axial direction of the main body section 2965 of the water sampling section 2956 (ie, in the front-rear direction).
  • one end i.e., the front end on the side closer to the inclined wall 2962 is bent upward, and the upper end thereof is a common drain pipe attachment part to which the common drain pipe 2332 is attached. It is 2964. Further, the other end (that is, the rear end) of the drainage section 2957 protrudes from the rear end of the water sampling section 2956 by about several millimeters to several centimeters (for example, 5 mm to 5 cm, more preferably 1 cm to 3 cm).
  • This water sampling/drainage unit 2931 is installed in the groove of the invert cell with the inclined wall 2962 of the water sampling section 2956 facing the flow of sewage (white arrow in FIG. 19).
  • the sewage flowing in the groove rides on the upper surface of the water sampling section 2956 along the inclined wall 2962 and flows into the internal space of the water sampling section 2956 through the small hole 2959 formed in the upper surface plate 2958.
  • the sewage liquid sending pump 2203 see FIG. 13
  • the sewage in the water sampling section 2956 is pumped up to each of the two water sampling pipes 2201 via the two water sampling pipe attachment sections 2963.
  • the diameter of the small hole 2959 provided in the water sampling section 2956 is the same as or smaller than the inner diameter of the water sampling tube 2201, so that solids of a size that cannot pass through the water sampling tube 2201 may be inside the water sampling section 2956. can be prevented from entering.
  • the liquid that has flowed through the common drain pipe 2332 that is, the sewage that has not been absorbed by the absorber 2280 in the sample collection section
  • the waste liquid discharged from the drainage part 2957 is transferred to the water sampling part. 2956 can be prevented from flowing into the small holes 2959.
  • one of the two walls located before and after the main body part 2965 of the water sampling part 2956 is a vertical wall 2961, and the other is a sloped wall 2962, but depending on the shape and dimensions of the installation location.
  • both of the two wall surfaces may be vertical walls 2961.
  • small holes 2959 similar to those provided in the top plate 2958 may be provided in the inclined wall 2962 or the vertical wall 2961 located on the front side of the water sampling portion 2956.
  • a water sampling/drainage unit 2931 as described above may be provided in place of the water sampling cup 802 as shown in FIG.
  • the sample collection device according to the first embodiment or the second embodiment is provided with two sets of water sampling pipes 1201 and 2201 and the first liquid pump 1203 or the sewage liquid pump 2203, or
  • the water sampling/draining unit 2931 has only one water sampling pipe attachment part 2963.
  • a sample collection device includes: a sample collection unit that collects samples from sewage facilities located at the sampling site; a pretreatment unit that performs predetermined pretreatment on the sample; a refrigerator that refrigerates a sample container containing the sample after the pretreatment; a transfer unit that transfers the sample container to the refrigerator; It has the following.
  • the sample collection device since the sample is automatically collected at the sampling site, there is no need for a specialist to regularly visit the sampling site to collect the sample, and the time and effort involved in sample collection is eliminated. and costs can be reduced. Furthermore, by pre-processing the collected sample, it is possible to save time and effort when performing a predetermined test on the sample. Furthermore, since the sample after pretreatment is automatically refrigerated, it is possible to prevent the sample from deteriorating due to high temperatures.
  • the sample collection device according to Paragraph 2 is the sample collection device according to Paragraph 1,
  • the pretreatment section concentrates the substance to be detected in the sample using a solid phase extraction method.
  • the sample collection device since the sample can be concentrated, the volume of the sample to be transported to the testing institution can be reduced, thereby reducing transportation costs.
  • the sample collection device according to Paragraph 3 is the sample collection device according to Paragraph 1,
  • the pretreatment section adds a component that inactivates pathogens to the sample.
  • sample collection device According to the sample collection device according to item 3, it is possible to reduce the risk of infection to workers when collecting samples from the refrigerator and transporting them to a testing institution for testing.
  • the sample collection device according to Paragraph 4 is the sample collection device according to Paragraph 3,
  • the ingredient that inactivates the pathogen is alcohol.
  • the pathogen can be effectively inactivated without decomposing the nucleic acid contained in the pathogen in the sample.
  • the sample collection device according to Paragraph 5 is the sample collection device according to Paragraph 1,
  • the pretreatment section adds the sample to a predetermined culture medium.
  • the sample collection device is the sample collection device according to any one of Paragraphs 1 to 5,
  • the sample collection section pumps up sewage from the sewage equipment over a predetermined sampling period, passes the sewage through an absorber, and then removes the components in the sewage absorbed by the absorber from the absorber.
  • the sample is collected by discharging it.
  • samples can be collected from sewage over a predetermined collection period, so even if the concentration of the detection target substance in sewage changes over time, A sample suitable for testing whether a detection target exists in the sewage can be collected.
  • the sample collection device according to Paragraph 7 is the sample collection device according to Paragraph 6,
  • the sample collection section squeezes out components in the sewage that have been absorbed by the absorber by pressurizing the absorber.
  • the components in the sewage that have been absorbed by the absorber can be easily eluted in a short time.
  • the sewage inspection method includes: a step of collecting a sample from a sewage facility located at the sampling site; a step of subjecting the sample to a predetermined pretreatment at the sampling location; refrigerating the sample after the pretreatment at the collection site; transporting the refrigerated sample from the collection site to a testing institution; an inspection step of inspecting whether a detection target exists in the sample at the inspection institution; It has the following.
  • the sewage testing method according to Section 8 by performing sample collection, pretreatment, and refrigeration at the sampling site, there is no need for workers to frequently visit the sampling site for sample collection. The effort and cost involved can be reduced. Furthermore, by pre-treating the sample at the collection site, it is possible to save time and effort during testing at a testing institution.
  • the sample collection device (Paragraph 9) The sample collection device according to Paragraph 9: an absorber holding tank that accommodates the absorber; a sewage supply unit that pumps up sewage from a sewage facility located at a sampling site over a predetermined sampling period and supplies the sewage to an absorber holding tank; After the collection period has elapsed, an absorber pressurizing section squeezes the absorber to squeeze out the sewage absorbed in the absorber; a sample collection unit that collects the sewage squeezed out by the absorber pressurization unit into a predetermined container as a sample; It has the following.
  • the sample collection device according to Section 10 is the sample collection device according to Section 9,
  • the absorbent holding tank holds substantially horizontally a perforated plate having a large number of small holes capable of holding the sewage water by surface tension in an upper opening of a concave absorbent holding part in which the absorbent body is accommodated;
  • the sewage water supplied from the sewage supply section is supplied to the upper surface of the perforated plate.
  • the sample collection device is the sample collection device according to paragraph 10, which includes: The upper opening of the absorbent body housing part and the porous plate are rectangular, The absorbent holding tank further has a concave shape for temporarily storing the sewage supplied from the sewage supply section, and has a rectangular upper opening, and one side of the upper opening is parallel to the upper opening of the absorbent storage section. A primary water storage part is provided opposite to one side, and the sewage supplied from the sewage supply part and overflowing from the upper opening of the primary water storage part is transmitted from the one side of the upper opening of the absorbent housing part to the perforated plate. It is supplied above.
  • the sample collection device according to Paragraph 12 is the sample collection device according to any one of Paragraphs 9 to 11,
  • the absorbent holding tank has a drain port for discharging the liquid in the absorbent holding tank, While the sewage is being supplied from the sewage supply unit to the absorber holding tank, the sample collection unit causes the liquid discharged from the drain port to flow down into the channel leading to the sewage equipment, While the sewage is being squeezed out from the absorber by the pressurizing section, the flow path is switched so that the liquid discharged from the drain port flows down into the flow path leading to the predetermined container. It is equipped with a section.
  • the sample collection device is the sample collection device according to any one of Paragraphs 9 to 12,
  • the sewage supply unit has a water sampling unit disposed within the sewage equipment,
  • the water sampling unit has a hollow main body, and a water sampling pipe connecting portion to which a water sampling pipe for sending sewage taken into the internal space of the main body to the absorber holding tank is connected,
  • the main body has a filter on a surface parallel to the flow direction of the sewage in the sewage equipment when installed in the sewage equipment, and the main body has a filter in the internal space through a large number of small holes provided in the filter. This is where the sewage flows into.

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PCT/JP2023/016157 2022-04-25 2023-04-24 試料採取装置 Ceased WO2023210597A1 (ja)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09101243A (ja) * 1995-10-03 1997-04-15 Yokogawa Electric Corp 採水器
JP2022003311A (ja) * 2020-06-23 2022-01-11 草野産業株式会社 水不溶性懸濁微粒子の濃縮方法及び下水中のウイルスの測定方法
JP7031957B1 (ja) * 2021-03-25 2022-03-08 株式会社島津テクノリサーチ 感染症検査方法
WO2022054878A1 (ja) * 2020-09-11 2022-03-17 日産化学株式会社 エンベロープを有するウイルスの付着が抑制された器具

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09101243A (ja) * 1995-10-03 1997-04-15 Yokogawa Electric Corp 採水器
JP2022003311A (ja) * 2020-06-23 2022-01-11 草野産業株式会社 水不溶性懸濁微粒子の濃縮方法及び下水中のウイルスの測定方法
WO2022054878A1 (ja) * 2020-09-11 2022-03-17 日産化学株式会社 エンベロープを有するウイルスの付着が抑制された器具
JP7031957B1 (ja) * 2021-03-25 2022-03-08 株式会社島津テクノリサーチ 感染症検査方法

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