WO2023165323A1

WO2023165323A1 - Nutritional composition, formula food product and use

Info

Publication number: WO2023165323A1
Application number: PCT/CN2023/075719
Authority: WO
Inventors: 刘洋; 解庆刚; 崔东影; 陆思宇; 蒋士龙; 张永久; 蔡方良; 冷友斌
Original assignee: 黑龙江飞鹤乳业有限公司
Priority date: 2022-03-02
Filing date: 2023-02-13
Publication date: 2023-09-07
Also published as: CN114431478A

Abstract

The present invention relates to a nutritional composition for use in a formula food product, wherein the nutritional composition comprises or consists of the following: (1) compound vitamins, which comprise vitamin A, which is partly or wholly derived from retinyl palmitate; vitamin E, which is partly or wholly derived from d-α-tocopheryl acetate and/or d-α-tocopherol; and vitamin B1, which is partly or wholly derived from thiamine hydrochloride; (2) compound minerals, which comprise iron, which is partly or wholly derived from ferrous gluconate; and zinc, which is partly or wholly derived from zinc gluconate and/or zinc citrate; and (3) calcium citrate. The present invention further relates to a formula food product containing the nutritional composition, and the non-therapeutic use of the nutritional composition or formula food product for enhancing the immunity of the body and improving the bioavailability of trace elements.

Description

Nutritional composition, formula food and use

technical field

The present invention generally belongs to the field of food. Specifically, the present invention relates to a nutritional composition for formula food, formula food comprising the nutritional composition, and uses of the nutritional composition or food. More specifically, the present invention relates to a nutritional composition comprising compound vitamins, compound minerals and calcium citrate, a formula food comprising the nutritional composition, especially infant formula food, preferably infant formula milk powder, Formulated milk or milk powder for children, or formulated milk or milk powder for adults, and the use of the nutritional composition or food for non-therapeutic purposes in enhancing immunity and promoting digestion and absorption.

Background technique

Healthy infants and children are the future and hope of a country and nation. Among the factors (heredity, nutrition, environment, exercise or training) that determine physical fitness and health, nutrition contributes about 70% and acts on the whole life course. A number of studies and evidence have shown that the nutritional status of infants and young children initiates and shapes the metabolic patterns of individuals throughout their lives, affecting their health status now and in adulthood. At the same time, nutritional interventions in infants and young children have great impact, good benefits, and high returns. If missed, it is difficult to make up for it. But when sufficient breastfeeding cannot be carried out due to some reasons, the infant needs to take the infant formula powder designed as the "gold standard" with breastmilk composition as food. Although infant formula milk powder is relatively close to breast milk, it cannot completely replace the nutritional value of breast milk. Nutrients are inevitably added during its production process, and the raw materials used in formula milk powder are subject to strict supervision. The vitamins and minerals in formula milk powder are crucial to the growth and immunity of fetuses, infants, and children, and usually consist of one or more combinations. For example, more than 90% of the zinc in infant formula milk powder comes from external additions, and the zinc (Zn) compounds that can be used to add infant milk powder are zinc sulfate, zinc gluconate, zinc oxide, zinc lactate, zinc citrate, zinc chloride and zinc acetate, their ratio and content are critical for optimal infant growth, as elements in different conjugated forms have different rates of digestion and absorption in the body. Nutritional fortifiers such as vitamins and minerals to be added in the production process of infant formula milk powder in my country must follow the source of compounds specified in GB 14880 "National Food Safety Standards for the Use of Food Nutritional Fortifiers", but the digestion and absorption rates of different compounds There are certain differences. Therefore, in order to improve the bioavailability of vitamins and minerals in formula The utilization rate, how to choose the form of nutrient compounds close to the ingredients in breast milk and its formula composition are worthy of further study.

Iron is an essential micronutrient for cells. Iron is mainly involved in the transport of oxygen in the body and the transfer process of the tissue respiratory chain. Iron participates in the formation of hemoglobin, myoglobin and cytochrome, and is an important element to maintain the normal hematopoietic function of infants. In infant formula milk powder More than 95% iron comes from external additions.

Zinc participates in the composition of various hormones, vitamins, proteins and enzymes, and participates in the catalytic, structural and regulatory functions in the body. Zinc plays an important role in the growth and development of infants. It is an activator of enzymes in the body, which can improve the immune function of infants, maintain the integrity of the cell membrane structure, and at the same time protect the skin and vision. 90% of infant formula milk powder The above zinc is added from outside.

Calcium is the main component of the bones and teeth of infants. It participates in the activation of various enzymes in the body, maintains the excitability of nerves and muscles, and participates in the transmission of nerve impulses. Calcium is also involved in blood coagulation, hormone secretion, maintenance of acid-base balance and regulation of body fluids. The normal physiological function of cells is involved in the regulation of the integrity and permeability of the biofilm. More than 50% of calcium in infant formula milk powder comes from external addition.

Vitamin A plays a vital role in many life processes in the body and has a wide range of physiological functions, including participation in visual processes, cell proliferation and differentiation, intercellular information exchange, organ and tissue growth, reproduction, and immune system functions, which can affect infants More than 99% of vitamin A in infant formula comes from external addition

The main physiological function of vitamin E is to act as a strong antioxidant to protect cell membranes from peroxidative damage; vitamin E is stored as a lipophilic compound in circulating lipoproteins, cell membranes, and adipose tissue; it reacts with free radicals and molecular oxygen, Protect polyunsaturated fatty acids and lipoproteins from peroxidation; at the same time, vitamin E also has the function of maintaining fertility, which is very important in the early stages of life and participates in the development from conception to postnatal development. More than 99% of infant formula milk powder Vitamin E is added externally.

In addition to playing an important physiological function as a coenzyme, vitamin _B1 also plays a significant role in maintaining the normal physiological functions of nerves, muscles, especially myocardium, maintaining normal appetite, gastrointestinal motility, and secretion of digestive juices. In infant formula milk powder More than 90% vitamin _B1 comes from external addition.

At present, in the existing infant formula milk powder, retinyl acetate is mostly used for vitamin A, dl-α-tocopheryl acetate is mostly used for vitamin E, thiamine nitrate is mostly used for vitamin _B1 , ferrous sulfate and zinc are mostly used for iron Zinc sulfate is used, and calcium carbonate is mostly used for calcium. However, there are some disadvantages and deficiencies in the above-mentioned nutritional components. For example, although ferrous sulfate is cost-effective, its bioavailability is low and it is harmful to the stomach of infants and young children. With severe irritation, the bioavailability of zinc sulfate and calcium carbonate is low.

Contents of the invention

An object of the present invention is to provide a nutritional composition for formula food, which can overcome the existing formula food, especially infant formula food, such as infant formula milk powder, children's formula modified milk or milk powder, or adult formula modified milk. Or the above-mentioned defects of milk powder, enhance the body's immunity and promote digestion and absorption.

Another object of the present invention is to provide a formula food containing the nutritional composition, especially infant formula food, preferably infant formula milk powder, children's formula milk or milk powder, or adult formula milk or milk powder.

Another object of the present invention is to provide the nutritional composition or food in enhancing the body's immunity and promoting digestion and absorption (or improving the bioavailability of trace elements), especially vitamin A, vitamin E and vitamin _B1 and iron, zinc and non-therapeutic purposes in the digestion and absorption of calcium.

In particular, the present invention is achieved by:

1. A nutritional composition for formula food, comprising or consisting of the following:

(1) Compound vitamins, which include:

Vitamin A, part or all of which is derived from retinyl palmitate, for example all of which is derived from retinyl palmitate;

Vitamin E, partly or wholly derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, e.g. wholly derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, e.g. whole source in d-alpha-tocopheryl acetate; and

Vitamin _B1 , partly or wholly derived from thiamine hydrochloride, for example entirely derived from thiamine hydrochloride,

(2) Compound minerals, which include:

Iron, a part or all of which is derived from ferrous gluconate, for example, iron is derived from ferrous gluconate or a combination of ferrous gluconate and one or more of ferrous fumarate, ferric citrate, and ferric pyrophosphate, such as all derived from ferrous gluconate, and

Zinc, partly or wholly derived from zinc gluconate and/or zinc citrate, such as wholly derived from zinc gluconate and/or zinc citrate, such as zinc derived from zinc gluconate, zinc citrate, or zinc citrate and glucose Zinc acid,

and

(3) Calcium citrate.

2. The nutritional composition according to item 1, wherein:

With respect to 1 mass part of calcium citrate,

The amount of vitamin A based on the active ingredient RE is 0.00008-0.007, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass;

The amount of vitamin E based on the active ingredient is 0.001-0.1, preferably 0.002-0.08, preferably 0.01-0.05 parts by mass; and

The amount of vitamin B1 in terms of active ingredients is 0.0001-0.01, preferably 0.0002-0.008, preferably 0.001-0.005 parts by mass.

3. The nutritional composition according to any one of items 1-2, wherein:

The compound vitamin further comprises one or more of the following: vitamin D, vitamin K ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , niacin, folic acid, pantothenic acid, vitamin C, and biotin,

Preferably, the compound vitamin further comprises vitamin D, vitamin K ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , niacin, folic acid, pantothenic acid, vitamin C, and biotin;

Preferably, when present,

Some or all of the vitamin D is derived from cholecalciferol, for example all from cholecalciferol, and/or

part or all of the vitamin _K1 is derived from phytonadiones, for example all of which are derived from phytonadiones, and/or

Some or all of the vitamin _B2 is derived from riboflavin, for example entirely from riboflavin, and/or

A part or all of vitamin _B6 is derived from pyridoxine hydrochloride and/or 5'-pyridoxal phosphate, for example all is derived from pyridoxine hydrochloride and/or 5'-pyridoxal phosphate, for example all is derived from pyridoxine hydrochloride; and / or

Some or all of the vitamin _B12 is derived from cyanocobalamin, for example entirely from cyanocobalamin; and/or

Some or all of the niacin is derived from niacinamide, for example all of it is derived from niacinamide; and/or

Some or all of the folic acid is derived from folic acid, such as entirely derived from folic acid; and/or

Some or all of the pantothenic acid is derived from calcium D-pantothenate, for example all is derived from D-calcium pantothenate; and/or

Part or all of vitamin C is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate; and/or

Part or all of biotin is derived from D-biotin, such as all is derived from D-biotin.

4. The nutritional composition according to item 3, wherein, when present, calculated as active ingredient relative to 1 part by mass of calcium citrate,

The amount of vitamin D is 0.000002-0.0002, preferably 0.000004-0.00015, preferably 0.00002-0.0001 parts by mass;

The amount of vitamin _K1 is 0.00001-0.001, preferably 0.00005-0.0008, preferably 0.0001-0.0005 parts by mass;

The amount of vitamin _B2 is 0.00008-0.005, preferably 0.0001-0.003, preferably 0.0005-0.002 parts by mass;

The amount of vitamin _B6 is 0.00006-0.007, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass;

The amount of vitamin _B12 is 0.0000002-0.00003, preferably 0.000001-0.00001, preferably 0.000002-0.000008 parts by mass;

The amount of niacin is 0.0006-0.05, preferably 0.001-0.04, preferably 0.005-0.02 parts by mass;

The amount of folic acid is 0.00001-0.002, preferably 0.00002-0.001, preferably 0.0001-0.0005 parts by mass;

The amount of pantothenic acid is 0.0005-0.03, preferably 0.001-0.02, preferably 0.005-0.01 parts by mass;

The amount of vitamin C is 0.015-1.2, preferably 0.02-1.0, preferably 0.10-0.50 parts by mass; and

The amount of biotin is 0.000001-0.0002, preferably 0.00001-0.0001, preferably 0.00001-0.00008 parts by mass.

5. The nutritional composition according to any one of items 1-4, wherein, relative to 1 part by mass of calcium citrate,

The amount of iron element is 0.0005-0.15, preferably 0.001-0.10, preferably 0.005-0.02 parts by mass; and

The amount of zinc element is 0.0005-0.10, preferably 0.001-0.05, preferably 0.005-0.02 parts by mass.

6. The nutritional composition according to any one of items 1-5, wherein:

The compound minerals further include one or more of the following elements: magnesium, manganese, iodine, copper, selenium;

Preferably, the compound minerals further include magnesium, manganese, iodine, copper, and selenium;

Preferably, when present,

Some or all of the magnesium is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate; and/or

Some or all of the manganese is derived from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all is derived from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all is derived from magnesium sulphate; and/or or

Some or all of the iodine is derived from potassium iodate, for example entirely from potassium iodate; and/or

Some or all of the copper is derived from copper sulphate and/or copper citrate and/or copper gluconate, for example all is derived from copper sulphate and/or copper citrate and/or copper gluconate, for example all is derived from copper sulphate; and/or or

Part or all of selenium is derived from sodium selenite, for example, all of it is derived from sodium selenite.

7. The composition according to any one of items 1-6, wherein, when present, relative to 1 part by mass of calcium citrate,

The amount of magnesium element is 0.001-0.2, preferably 0.005-0.1, preferably 0.01-0.05 parts by mass;

The amount of manganese element is 0.000006-0.0006, preferably 0.00001-0.0004, preferably 0.00005-0.0002 parts by mass;

The amount of iodine element is 0.000003-0.0015, preferably 0.00005-0.001, preferably 0.0001-0.0005 parts by mass;

The amount of copper element is 0.00004-0.006, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass; and

The amount of selenium element is 0.000001-0.0002, preferably 0.000002-0.0002, preferably 0.00001-0.0001 parts by mass.

8. The nutritional composition according to any one of items 1-7, wherein each gram of multivitamin comprises:

Vitamin A: 550-4600 μg RE, preferably 1080-3080 μg RE,

Vitamin E: 9.0-73.0mg α-TE, preferably 18-49mg α-TE,

Vitamin _B1 : 1.0-7.0mg, preferably 1.9-4.8mg,

Vitamin D: 18.0-136.0 μg, preferably 36.6-91.0 μg,

Vitamin _K1 : 70-670 μg, preferably 138-448 μg,

Vitamin _B2 : 550-3500 μg, preferably 890-2600 μg,

Vitamin _B6 : 0.45-5.0mg, preferably 0.8-3.4mg,

Vitamin _B12 : 1.8-16.0 μg, preferably 3.6-11.0 μg,

Niacin: 4.5-37.5mg, preferably 8.0-26.0mg,

Folic acid: 90-1100μg, preferably 180-790μg,

Pantothenic acid: 3.5-25.0mg, preferably 6.0-17.6mg,

Vitamin C: 105-900mg, preferably 185-650mg, and

Biotin: 12.0-130.0 μg, preferably 21.0-86.8 μg;

and / or

Each gram of mixed minerals includes the following elements:

Iron: 6.0-89.0mg, preferably 12.0-59.5mg,

Zinc: 3.0-75.0mg, preferably 7.0-55.2mg,

Magnesium: 10.0-120mg, preferably 19.8-79.0mg,

Manganese: 45-400 μg, preferably 84-294 μg,

Iodine: 20-900 μg, preferably 40-560 μg,

Copper: 300-4200 μg, preferably 600-2800 μg, and

Selenium: 10-130 μg, preferably 24-98 μg.

9. The nutritional composition according to any one of items 1-8, wherein:

Relative to 1 mass part of calcium citrate, the amount of compound vitamins is 0.15-1.33 mass parts, preferably 0.2-1.1 mass parts; and

The amount of the compound mineral is 0.15-1.33 parts by mass, preferably 0.2-1.1 parts by mass, relative to 1 part by mass of calcium citrate.

10. A formula food, preferably infant formula food, preferably infant formula milk powder, children's formula modified milk or milk powder, adult formula modified milk or milk powder, which contains the nutrition described in any one of items 1-9 Composition, preferably the added amount of the nutritional composition in the formula food is 0.6-1.8 mass%, preferably 0.7-1.4 mass%.

11. The nutritional composition as described in any one of items 1-9 or the formula food as described in item 10 can enhance immunity and promote digestion and absorption, especially for vitamin A, vitamin E and vitamin B1 as well as iron, zinc and calcium Non-therapeutic purposes in digestion and absorption.

The present invention evaluates the effects of the nutritional composition containing the compound vitamins and compound minerals of the present invention on enhancing body immunity and promoting digestion and absorption. The inventors have found through a large number of long-term experimental studies that by using special vitamin A (retinyl palmitate), vitamin E (d-α-acetic acid) tocopherol and/or d-alpha-tocopherol) and vitamin B1 (thiamine hydrochloride) and iron (ferrous gluconate), zinc (zinc citrate and/or zinc gluconate) and calcium (calcium citrate), Compared with vitamin A (retinyl acetate), vitamin E (dl-α-tocopheryl acetate) and vitamin _B1 (thiamine nitrate) and iron (sulfuric acid) commonly used in existing formula foods, especially infant formula foods ferrous), zinc (zinc sulfate) and calcium (calcium carbonate), the present invention has the functions of obviously enhancing the immunity of the body, reducing the risk of various diseases, and promoting digestion and absorption at the same time, especially for vitamin A, vitamin E and vitamin _B1 And the function of the digestion and absorption of iron, zinc and calcium, thereby overcome the disadvantage and deficiency in the existing formula food especially infant formula food, this is the effect that those skilled in the art can't expect from the prior art, for improving Existing formulas, especially formulas for infants and young children, are of great significance.

Detailed ways

definition

Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, but in case of conflict, the definitions in this specification shall prevail.

As used herein, the following terms have the following meanings.

The term "infant" refers to the population of 0 to 6 months of age.

The term "older infant" refers to the population of 6 to 12 months of age.

The term "young child" refers to the population of 12 to 36 months of age.

The term "infant" refers to the population of 0-36 months of age.

The term "children" refers to people aged 4 to 18 years.

The term "adult" refers to people over the age of 18, including pregnant women and middle-aged and elderly people.

The term "infant formula" as used herein encompasses infant formula, follow-on formula and toddler formula. Generally, infant formula is used as a breast milk substitute from the birth of a baby, formula for older infants is used as a breast milk substitute from 6-12 months after birth, and formula for young children is used as a substitute for breast milk from 12-36 months after birth. Breastmilk substitutes.

The term "infant formula" refers to milk and milk protein products or soybeans and soybean protein products as the main raw materials, adding appropriate amount of vitamins, minerals and/or other ingredients, and produced and processed by only physical methods in liquid or powder form product. It is suitable for normal infants, and its energy and nutrients can meet the normal nutritional needs of infants aged 0-6 months.

The term "following-up formula" refers to milk and milk protein products or soybeans and soybean protein products as the main raw materials, adding appropriate amount of vitamins, minerals and/or other ingredients, produced only by physical methods Liquid or powder products made by production and processing. It is suitable for older babies, and its energy and nutrients can meet part of the nutritional needs of normal older babies aged 6-12 months.

The term "infant formula food" refers to milk and milk protein products or soybeans and soybean protein products as the main raw materials, adding appropriate amount of vitamins, minerals and/or other ingredients, and produced and processed by only physical methods in liquid or powder form product. Suitable for young children, its energy and nutrients can meet part of the nutritional needs of normal children aged 12'-36 months.

The term "Children's Formula Modified Milk or Milk Powder" refers to milk and milk protein products as the main raw materials, adding appropriate amount of vitamins, minerals and/or other ingredients, which are produced and processed by physical methods and are suitable for children. or powdered products.

The term "adult formula modified milk or milk powder" refers to a liquid product suitable for adults that is produced and processed by physical methods, with milk and milk protein products as the main raw materials, adding appropriate amounts of vitamins, minerals and/or other ingredients. or powdered products.

The term "breast milk" is to be understood as mother's breast milk or colostrum.

The term "exclusively breastfed infant or young child" has its usual meaning and refers to an infant whose nutritional and/or energy is derived substantially from human breast milk.

The term "infant/older infant/toddler mainly fed with infant formula" has the usual meaning, referring to infant formula, older Infant milk or growing-up milk for infants or young children. The term "mainly" means at least 50%, such as at least 75%, of those nutrients and/or energy. Unless otherwise specified, the percentages (%) in this specification are all percentages by weight (% by weight).

All numbers or expressions referring to amounts of components, process conditions, etc. used in the specification and claims are to be understood as modified by "about" in all instances. The term "about" when referring to an amount or a numerical range means that the indicated amount or numerical range is an approximation within experimental variability (or within statistical experimental error) such that the amount or numerical range may be within the stated amount or numerical range varies between eg ±5.

All ranges referring to the same component or property are inclusive of endpoints, which are independently combinable. Since these ranges are continuous, they include every value between the minimum and maximum values. It should also be understood that any numerical range recited herein is intended to include all subranges within that range.

Where the invention is directed to ranges of physical properties such as molecular weight or to chemical properties, all combinations and subcombinations of ranges and embodiments therein are intended to be included. The term "comprising" (and related terms such as "comprising" or "containing" or "having" or "including") includes embodiments that An embodiment is, for example, any combination of matter, component, method, or process, etc., that "consists of" or "consists essentially of" the described features.

The "and/or" used in the specification and claims should be understood as "either or both" of the associated components, that is, the components exist jointly in some cases and exist separately in other cases. Multiple components listed with "and/or" should be construed in the same fashion, ie "one or more" of the associated components. Other components may optionally be present other than the components specifically identified by the "and/or" clause, whether related or unrelated to those components specifically identified. Thus, as a non-limiting example, reference to "A and/or B", when used in conjunction with an open-ended word such as "comprising", may, in one embodiment, refer to only A (optionally including in addition to B In another embodiment, it may refer to only B (optionally including components other than A); in yet another embodiment, it may refer to A and B (optionally including other components) and so on.

It should be understood that in any method claimed herein that includes more than one step or act, the order of the method steps and acts is not necessarily limited to the order of the method steps and acts recited, unless expressly indicated to the contrary.

Abbreviations used herein have their usual meanings in the fields of food, biology and chemistry.

The invention will now be described in more detail. It should be noted that the various aspects, features, embodiments, examples and advantages described in this application may be compatible and/or may be combined together.

nutritional composition

In one aspect, the present invention provides a nutritional composition comprising or consisting of:

(1) Compound vitamins, which include:

Vitamin A, part or all of which is derived from retinyl palmitate;

Vitamin E derived in part or in whole from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol; and

Vitamin B ₁ , part or all of which is derived from thiamine hydrochloride,

(2) Compound minerals, which include:

Iron, some or all of which is derived from ferrous gluconate, and

Zinc, part or all of which is derived from zinc gluconate and/or zinc citrate,

and

(3) Calcium citrate.

The inventors found that compared to the existing formula foods, especially vitamins and minerals commonly used in infant formula foods, the nutritional composition of the present invention has (but is not limited to) the following functions: promote the perfection of the body's immune system and enhance the body's immunity , reduce the risk of various diseases, etc., and at the same time promote the digestion and absorption of nutritional components:

1) Enhance the immunity of infants and young children: due to the immature immune system of infants and young children, and lack of awareness of hygiene and disease, the ability to resist diseases is weaker than that of adults, and the resistance to unknown diseases is even worse. Therefore, to strengthen the immune system, It is especially important for infants to provide resistance, and the nutritional composition can enhance the immunity of infants and improve the defense ability of infants against diseases.

2) Promote digestion and absorption of infants and young children: The degree of absorption of nutritional components by infants directly affects the development of various organs, mental development, physical health, and the strength of the body's immunity. Therefore, each nutritional group The digestion and absorption of food is of great significance to the healthy development of infants and young children. The nutritional composition is more suitable for infants to absorb and digest nutritional components, and has better bioavailability.

Each component of the nutritional composition will be described in detail below.

multivitamins

The compound vitamins include vitamin A, vitamin E and vitamin B ₁ .

Part or all of vitamin A is derived from retinyl palmitate, for example, all vitamin A is derived from retinyl palmitate.

Preferably, relative to 1 mass part of calcium citrate, the amount of vitamin A in terms of active ingredients (i.e. RE) can be 0.00008-0.007 in mass parts (i.e., when calcium citrate is 1 gram, the amount of vitamin A Can be 80-7000 μ g RE), preferably 0.0001-0.005, preferably 0.0005-0.002, for example can be 0.00008, 0.00009, 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.00 09, 0.0010, 0.0015, 0.0020, 0.0025 , 0.0030, 0.0035, 0.0040, 0.0045, 0.0050, 0.0055, 0.0060, 0.0065, 0.0070, or a range defined by any two thereof and any values and subranges subsumed within that range.

A part or all of vitamin E is derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, for example all is derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, for example all is derived from d-alpha-tocopheryl acetate.

Preferably, with respect to 1 mass part of calcium citrate, the amount of vitamin E in terms of active ingredients can be 0.001-0.1, preferably 0.002-0.08, preferably 0.01-0.05, such as 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.010, 0.015, 0.020, 0.025, 0.030, 0.035, 0.040, 0.045, 0.050, 0.055, 0.060, 0.065, 0 .070, 0.075, 0.080, 0.085, 0.090, 0.095, 0.10, or ranges bounded by any two thereof and any values and subranges subsumed within that range.

Part or all of vitamin _B1 is derived from thiamine hydrochloride, for example, all of it is derived from thiamine hydrochloride.

Preferably, relative to 1 mass part of calcium citrate, the amount of vitamin B1 in terms of active ingredients can be 0.0001-0.01, preferably 0.0002-0.008, preferably 0.001-0.005, such as 0.0001, 0.00015, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.0010, 0.0015, 0.0020, 0.0025, 0.0030, 0.0035, 0.0040, 0.0045, 0.0050, 0.0055, 0. 0060, 0.0065, 0.0070, 0.0075, 0.0080, 0.0085, 0.0090, 0.0095, 0.010, or a range bounded by any two thereof, and any values and subranges subsumed within that range.

The present inventors have found that when the amount of defined vitamin A, vitamin E and vitamin B1 is within the above range, the nutritional composition of the present invention can better promote the perfection of the body's immune system, enhance the body's immunity, and reduce the risk of various diseases. risks, etc., while promoting the digestion and absorption of nutritional components.

In addition to vitamin A (i.e. retinyl palmitate), vitamin E (i.e. d-alpha-tocopheryl acetate and/or d-alpha-tocopherol) and vitamin _B1 (i.e. thiamine hydrochloride) from defined sources , the multivitamins can optionally further comprise vitamin A (such as retinyl acetate), vitamin E (dl-α-tocopheryl acetate) and vitamin B1 (that is, thiamine nitrate) from other sources commonly used in the art. white).

The compound vitamin may further include one or more of the following: vitamin D, vitamin K ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , niacin, folic acid, pantothenic acid, vitamin C, and biotin. For example, in one embodiment, in addition to the defined vitamin A, vitamin E and vitamin _B1 , the compound vitamin further comprises vitamin D, vitamin _K1 , vitamin _B2 , vitamin _B6 , vitamin _B12 , niacin, folic acid, pantothenic acid, vitamin C, and biotin.

In one embodiment, the multivitamins may further include vitamin D.

Preferably, part or all of the vitamin D is derived from cholecalciferol, for example all of it is derived from cholecalciferol.

Preferably, when present, the amount of vitamin D in terms of active ingredient may be 0.000002-0.0002, preferably 0.000004-0.00015, preferably 0.000004-0.00015, preferably 0.00002-0.0001, such as 0.000002, 0.000003, 0.000004, 0.000005, 0.000006, 0.000007, 0.000008, 0.000009, 0.000010, 0.000015, 0.000020, 0.00 0025, 0.000030, 0.000035, 0.000040, 0.000045, 0.000050, 0.000055, 0.000060, 0.000065, 0.000070, 0.000075 , 0.000080, 0.000085, 0.000090, 0.000095, 0.00010, 0.00011, 0.00012, 0.00013, 0.00014, 0.00015, 0.00016, 0.00017, 0.00018, 0.00019, 0.00 020, or the range defined by any two of them and any value and sub- scope.

In one embodiment, the compound vitamin may further include vitamin K ₁ .

Preferably, part or all of vitamin _K1 is derived from phytonadione, for example all is derived from phytonadione.

Preferably, when present, the amount of vitamin K ₁ in terms of active ingredient may be 0.00001-0.001 in parts by mass, preferably 0.00005-0.0008, preferably 0.0001-0.0005, for example may be 0.00001, relative to 1 part by mass of calcium citrate ,0.00002,0.00003,0.00004,0.00005,0.00006,0.00007,0.00008,0.00009,0.00010,0.00015,0.00020,0.00025,0.00030,0.00035,0.00040, 0.00045, 0.00050, 0.00055, 0.00060, 0.00065, 0.00070, 0.00075, 0.00080, 0.00085, 0.00090 , 0.00095, 0.0010, or ranges defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the compound vitamin may further include vitamin B ₂ .

Preferably, a part or all of vitamin _B2 is derived from riboflavin, for example all of it is derived from riboflavin.

Preferably, when present, the amount of vitamin _B2 in terms of active ingredient may be 0.00008-0.005, preferably 0.0001-0.003, preferably 0.0005-0.002, for example may be 0.00008, relative to 1 mass part of calcium citrate ,0.00009,0.0001,0.0002,0.0003,0.0004,0.0005,0.0006,0.0007,0.0008,0.0009,0.0010,0.0015,0.0020,0.0025,0.0030,0.0035,0.0040, 0.0045, 0.0050, or a range defined by any two thereof and including Any value within that range and subranges.

In one embodiment, the compound vitamin may further include vitamin B ₆ .

Preferably, part or all of vitamin _B6 is derived from pyridoxine hydrochloride and/or pyridoxal 5'-phosphate, for example all is derived from pyridoxine hydrochloride and/or pyridoxal 5'-phosphate, for example all is derived from pyridoxine hydrochloride Pyridoxine.

Preferably, when present, the amount of vitamin _B6 in terms of active ingredient may be 0.00006-0.007, preferably 0.0001-0.005, preferably 0.0005-0.002, for example may be 0.00006, relative to 1 mass part of calcium citrate , 0.00007, 0.00008, 0.00009, 0.0001, 0.0002, 0.0003, 0.0004, 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.0010, 0.0015, 0.0020, 0.0025, 0.003 0, 0.0035, 0.0040, 0.0045, 0.0050, 0.0055, 0.0060, 0.0065, 0.0070 , or a range defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the compound vitamins may further include vitamin B ₁₂ .

Optionally, part or all of the vitamin _B12 is derived from cyanocobalamin, for example entirely derived from cyanocobalamin.

Preferably, when present, the amount of vitamin B ₁₂ in terms of active ingredient may be 0.0000002-0.00003 parts by mass, preferably 0.000001-0.00001, preferably 0.000002-0.000008, for example may be 0.0000002, relative to 1 part by mass of calcium citrate , 0.0000003, 0.0000004, 0.0000005, 0.0000006, 0.0000007, 0.0000008, 0.0000009, 0.0000010, 0.0000015, 0.0000020, 0.0000025, 0.0000030, 0.0000035, 0.0000040, 0.0000045, 0.0000050, 0.0000055, 0.0000060, 0.0000065, 0.0000070, 0.0000075, 0.0000080, 0.0000085, 0.0000090, 0.0 000095 , 0.000010, 0.000011, 0.000012, 0.000013, 0.000014, 0.000015, 0.000016, 0.000017, 0.000018, 0.000019, 0.000020, 0.000025, 0.000030, or Any two defined ranges and any values and subranges subsumed within that range.

In one embodiment, the multivitamins may further include niacin.

Preferably, part or all of the niacin is derived from niacinamide, for example all of it is derived from niacinamide.

Preferably, when present, the amount of niacin in terms of active ingredient may be 0.0006-0.05, preferably 0.001-0.04, preferably 0.005-0.02, for example may be 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.010, 0.015, 0.020, 0.025, 0.030, 0.035, 0.040, 0.04 5, 0.050, or limited by any two of them range and any values and subranges subsumed within that range.

In one embodiment, the multivitamin may further comprise folic acid.

Preferably, some or all of the folic acid is derived from folic acid (pteroylglutamic acid), for example entirely derived from acid (pteroylglutamic acid).

Preferably, when present, the amount of folic acid in terms of active ingredient may be 0.00001-0.002, preferably 0.00002-0.001, preferably 0.0001-0.0005, for example may be 0.00001, 0.00002 in parts by mass relative to 1 part by mass of calcium citrate ,0.00003,0.00004,0.00005,0.00006,0.00007,0.00008,0.00009,0.00010,0.00015,0.00020,0.00025,0.00030,0.00035,0.00040,0.00045, 0.00050, 0.00055, 0.00060, 0.00065, 0.00070, 0.00075, 0.00080, 0.00085, 0.00090, 0.00095 , 0.0010, 0.0011, 0.0012, 0.0013, 0.0014, 0.0015, 0.0016, 0.0017, 0.0018, 0.0019, 0.0020, or ranges defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the multivitamins may further include pantothenic acid.

Preferably, part or all of the pantothenic acid is derived from calcium D-pantothenate, for example, all of it is derived from calcium D-pantothenate.

Preferably, when present, the amount of pantothenic acid in terms of active ingredient may be 0.0005-0.03, preferably 0.001-0.02, preferably 0.005-0.01 in parts by mass relative to 1 part by mass of calcium citrate, for example may be 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.0010, 0.0015, 0.0020, 0.0025, 0.0030, 0.0035, 0.0040, 0.0045, 0.0050, 0.0055, 0.0060, 0.0065, 0. 0070, 0.0075, 0.0080, 0.0085, 0.0090, 0.0095, 0.010, 0.011, 0.012, 0.013, 0.014, 0.015, 0.016, 0.017, 0.018, 0.019, 0.020, 0.021, 0.022, 0.023, 0.024, 0.025, 0.026, 0.027, 0.028, 0.029, 0.030, or any two of them range and include Arbitrary values and subranges within the range.

In one embodiment, the multivitamins may further include vitamin C.

Preferably, part or all of vitamin C is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate.

Preferably, when present, the amount of vitamin C in terms of active ingredient may be 0.015-1.2, preferably 0.02-1.0, preferably 0.10-0.50, for example may be 0.015, relative to 1 mass part of calcium citrate , 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, 0.40, 0.45, 0.50, 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85, 0.90 , 0.95, 1.0, 1.10, 1.15, 1.20, or a range defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the multivitamins may further contain biotin.

Preferably, part or all of the biotin is derived from D-biotin, such as all is derived from D-biotin.

Preferably, when present, the amount of biotin in terms of active ingredient may be 0.000001-0.0002, preferably 0.00001-0.0001, preferably 0.00001-0.00008, for example may be 0.000001, 0.0000015, 0.000002, 0.000003, 0.000004, 0.000005, 0.000006, 0.000007, 0.000008, 0.000009, 0.000010, 0.000015, 0.000020, 0.000025, 0.0 00030, 0.000035, 0.000040, 0.000045, 0.000050, 0.000055, 0.000060, 0.000065, 0.000070, 0.000075, 0.000080, 0.000085, 0.000090, 0.000095, 0.00010, 0.00011, 0.00012, 0.00013, 0.00014, 0.00015, 0.00016, 0.00017, 0.00018, 0.00019, 0.00020, or a range defined by any two thereof and encompassed within such range Arbitrary values and subranges of .

Optionally, the compound vitamin may further contain other nutritional components, such as taurine, L-carnitine, inositol, choline chloride and the like.

For example, in one embodiment, the multivitamins may further include taurine.

Preferably, when present, the amount of taurine in terms of active ingredient may be 0.01-1.0, preferably 0.05-0.2 by mass, for example may be 0.01, 0.02, 0.03, 0.04, relative to 1 part by mass of calcium citrate 0.05, 0.06, 0.07, 0.08, 0.09, 0.10, 0.15, 0.25, 0.30, 0.35, 0.40, 0.50, 0.55, 0.65, 0.70, 0.80, 0.90, 0.95, 1.0, or or, or or A range defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the compound vitamin further includes auxiliary materials.

Preferably, the auxiliary material can be lactose or maltodextrin or other food raw materials that meet regulatory requirements.

The excipients are present in an amount to aid in the effective dispersion of the vitamin components mentioned. Preferably, when present, relative to 1 mass part of calcium citrate, the amount of the excipient contained in the compound vitamin can be 0.01-10 mass parts, for example, it can be 0.01, 0.02, 0.03, 0.04, 0.05, 0.06 0.07, 0.08, 0.10, 0.10, 0.15, 0.20, 0.0.30, 0.35, 0.40, 0.50, 0.55, 0.60, 0.70, 0.75, 0.85, 0.95, 1.0, 1.0, 2.0, 2.5, 2.5 , 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, or the range defined by any two thereof and any value and sub- scope.

In a preferred embodiment, the compound vitamins include the following:

Vitamin A, part or all of which is derived from retinyl palmitate, preferably all of which is derived from retinyl palmitate;

Vitamin E, part or all of which is derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, preferably all of it is derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol;

Vitamin B ₁ , part or all of which is derived from thiamine hydrochloride, preferably all of which is derived from thiamine hydrochloride;

Vitamin D, preferably derived from cholecalciferol;

Vitamin K ₁ , preferably derived from phytonadione;

Vitamin _B2 , preferably derived from riboflavin;

Vitamin _B6 , preferably derived from pyridoxine hydrochloride and/or pyridoxal 5'-phosphate;

Vitamin B ₁₂ , preferably derived from cyanocobalamin;

Niacin, preferably derived from niacinamide;

Folic acid, preferably derived from folic acid (pteroylglutamic acid);

Pantothenic acid, preferably derived from calcium D-pantothenate;

Vitamin C, preferably derived from sodium L-ascorbate and/or L-ascorbic acid;

biotin, preferably derived from D-biotin; and

Optionally, taurine.

In a further preferred embodiment, the compound vitamins include the following:

Vitamin A, all derived from retinyl palmitate;

Vitamin E, all derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol;

Vitamin B ₁ , all derived from thiamine hydrochloride;

Vitamin D, which is derived from cholecalciferol;

Vitamin K ₁ derived from phytonadione;

Vitamin _B2 , which is derived from riboflavin;

Vitamin _B6 derived from pyridoxine hydrochloride and/or pyridoxal 5'-phosphate;

Vitamin B ₁₂ derived from cyanocobalamin;

Niacin, which is derived from niacinamide;

Folic acid, which is derived from folic acid (pteroylglutamate);

Pantothenic acid, which is derived from calcium D-pantothenate;

Vitamin C derived from sodium L-ascorbate and/or L-ascorbic acid;

Biotin, derived from D-biotin; and

Optionally, taurine.

In one embodiment, each gram of compound vitamin in the nutritional composition includes the following ingredients (calculated as active ingredients):

Vitamin A: 550-4600 μg RE, preferably 1080-3080 μg RE, e.g. 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400 , 1450, 1500, 1550, 1600, 1650, 1700, 1750, 1800, 1850, 1900, 1950, 2000, 2050, 2100, 2150, 2200, 2250, 2300, 2350, 2400, 2450, 2500, 2550, 2600, 2650 ,2700,2750,2800,2850,2900,2950,3000,3050,3100,3150,3200,3250,3300,3350,3400,3450,3500,3550,3600,3650,3700,3750,3800,3850, 3900 , 3950, 4000, 4050, 4100, 4150, 4200, 4250, 4300, 4350, 4400, 4450, 4500, 4550, 4600, or ranges defined by any two thereof and any values and subranges subsumed within that range;

Vitamin E: 9.0-73.0 mg alpha-TE, preferably 18-49 mg alpha-TE, eg 9.0, 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0, 29.0, 30.0, 31.0, 32.0, 33.0, 34.0, 35.0, 36.0, 37.0, 38.0, 39.0, 40.0, 41.0, 42.0, 43.0, 44.0, 45.0, 46.0, 47.0, 4 8.0, 49.0, 50.0, 51.0, 52.0, 53.0, 54.0, 55.0, 56.0, 57.0, 58.0, 59.0, 60.0, 61.0, 62.0, 63.0, 64.0, 65.0, 66.0, 67.0, 68.0, 69.0, 70.0, 71.0, 72.0, 7 3.0mg α-TE, or a range defined by any two thereof, and any values and subranges subsumed within that range;

Vitamin _B1 : 1.0-7.0 mg, preferably 1.9-4.8 mg, eg 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0 mg or ranges defined by any two thereof and encompassed within any value within that range and subranges;

Vitamin D: 18.0-136.0 μg, preferably 36.6-91.0 μg, eg 18.0, 18.5, 19.0, 19.5, 20.0, 20.5, 21.0, 21.5, 22.0, 22.5, 23.0, 23.5, 24.0, 24.5, 25.0, 25.5, 26.0, 26.5 ,27.0,27.5,28.0,28.5,29.0,29.5,30.0,30.5,31.0,32.0,33.0,34.0,35.0,36.0,37.0,38.0,39.0,40.0,41.0,42.0,43.0,44.0,45.0,46.0, 47.0 , 48.0, 49.0, 50.0, 51.0, 52.0, 53.0, 54.0, 55.0, 56.0, 57.0, 58.0, 59.0, 60.0, 61.0, 62.0, 63.0, 64.0, 65.0, 66.0, 67.0, 68.0, 69.0, 70.0, 71.0, 72.0, 73.0, 74.0, 75.0, 76.0, 77.0, 78.0, 79.0, 80.0, 8 1.0, 82.0, 83.0, 84.0, 85.0, 86.0, 87.0, 88.0, 89.0, 90.0, 91.0, 92.0, 93.0, 94.0, 95.0, 96.0, 97.0, 97.5, 98.0, 98.5, 99.0, 99.5, 100.0, 100.5, 101. 0, 101.5, 102.0, 102.5, 103.0, 103.5, 104.0, 104.5, 105.0, 105.5, 106.0, 106.5, 107.0, 107.5, 108.0, 108.5, 109.0, 109.5, 110.0, 110.5, 111.0, 111.5, 1 12.0, 112.5, 113.0, 113.5, 114.0, 114.5, 115.0, 115.5, 116.0, 116.5, 117.0, 117.5, 118.0, 118.5, 119.0, 119.5, 120.0, 120.5, 121.0, 121.5, 122.0, 122.5, 123.0, 123.5, 124.0, 1 24.5, 125.0, 125.5, 126.0, 126.5, 127.0, 127.5, 128.0, 128.5, 129.0, 129.5, 130.0, 130.5, 131.0, 132.0, 133.0, 134.0, 135.0, 136.0 μg, or ranges defined by any two thereof and any values and subranges subsumed therein;

Vitamin _K1 : 70-670 μg, preferably 138-448 μg, for example 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380, 385, 390, 395, 400, 405, 410, 415, 420, 425, 430, 435, 440, 445, 450, 455, 460, 465, 470, 475, 480, 485, 490, 495, 500, 505, 510, 515, 520, 525, 530, 535, 540, 545, 550, 555, 560, 565, 570, 575, 580, 585, 590, 595, 600, 605, 610, 615, 620, 625, 630, 635, 640, 645, 650, 655, 660, 665, 670 μg, or a range defined by any two thereof and any values and subranges subsumed within that range;

Vitamin _B2 : 550-3500 μg, preferably 890-2600 μg, for example 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500, 1550, 1600, 1650, 1700, 1750, 1800, 1850, 1900, 1950, 2000, 2050, 2100, 2150, 2200, 2250, 2300, 2350, 2400, 2450, 2500, 2550, 2600, 2 650, 2700, 2750, 2800, 2850, 2900, 2950, 3000, 3050, 3100, 3150, 3200, 3250, 3300, 3350, 3400, 3450, 3500 μg, or the range defined by any two thereof and included within the range Arbitrary values and subranges;

Vitamin _B6 : 0.45-5.0 mg, preferably 0.8-3.4 mg, eg 0.45, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0 mg, or a range defined by any two thereof and any values and subranges subsumed within that range;

Vitamin _B12 : 1.8-16.0 μg, preferably 3.6-11.0 μg, eg 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11.0, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, 12.0, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 12.7, 12.8, 12.9, 13.0, 13.1, 13.2, 13.3, 1 3.4、 13.5, 13.6, 13.7, 13.8, 13.9, 14.0, 14.1, 14.2, 14.3, 14.4, 14.5, 14.6, 14.7, 14.8, 14.9, 15.0, 15.1, 15.2, 15.3, 15.4, 15.5, 15.6, 15.7, 15.8, 1 5.9, 16.0 μg, or the range defined by any two thereof and any value and subrange subsumed within that range;

Niacin: 4.5-37.5 mg, preferably 8.0-26.0 mg, eg 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, 10.5, 11.0, 11.5, 12.0, 12.5, 13.0 ,13.5,14.0,14.5,15.0,15.5,16.0,16.5,17.0,17.5,18.0,18.5,19.0,19.5,20.0,20.5,21.0,21.5,22.0,22.5,23.0,23.5,24.0,24.5,25.0, 25.5 , 26.0, 26.5, 27.0, 27.5, 28.0, 28.5, 29.0, 29.5, 30.0, 30.5, 31.0, 31.5, 32.0, 32.5, 33.0, 33.5, 34.0, 34.5, 35.0, 35.5, 36.0, 36.5, 37.0, 37.5mg , or a range defined by any two thereof and any values and subranges subsumed within that range;

Folic acid: 90-1100 μg, preferably 180-790 μg, eg 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380, 385, 390, 395, 400, 405, 410, 415, 420, 425, 430, 435, 440, 445, 450, 455, 460, 465, 470, 475, 480, 485, 490, 495, 500, 505, 510, 515, 520, 525, 530, 535, 540, 545, 550, 555, 560, 565, 570, 575, 580, 585, 590, 595, 600, 605, 610, 615, 620, 625, 630, 635, 640, 645, 650, 655, 660, 665, 670, 675, 680, 685, 690, 695, 700, 705, 710, 715, 720, 725, 730, 735, 740, 745, 750, 755, 760, 765, 770, 775, 780, 785, 790, 795, 800, 805, 810, 815, 820, 825, 830, 835, 840, 845, 850, 855, 860, 865, 870, 875, 880, 885, 890, 895, 900, 905, 910, 915, 920, 925, 930, 935, 940, 945, 950, 955, 960, 965, 970, 975, 980, 985, 990, 995, 1005, 1010, 1015, 1020, 1025, 1030, 1035, 1045, 1050, 1055, 1060, 1065, 1075, 1085, 1095, 1100 μg, or from ranges defined by any two thereof and any values and subranges subsumed within that range;

Pantothenic acid: 3.5-25.0 mg, preferably 6.0-17.6 mg, eg 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11.0, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, 12.0, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 1 2.7, 12.8, 12.9, 13.0, 13.1, 13.2, 13.3, 13.4, 13.5, 13.6, 13.7, 13.8, 13.9, 14.0, 14.1, 14.2, 14.3, 14.4, 14.5, 14.6, 14.7, 14.8, 14.9, 15.0, 15.1, 1 5.2、 15.3, 15.4, 15.5, 15.6, 15.7, 15.8, 15.9, 16.0, 16.1, 16.2, 16.3, 16.4, 16.5, 16.6, 16.7, 16.8, 16.9, 17.0, 17.1, 17.2, 17.3, 17.4, 17.5, 17.6, 1 7.7, 17.8, 17.9, 18.0, 18.1, 18.2, 18.3, 18.4, 18.5, 18.6, 18.7, 18.8, 18.9, 19.0, 19.1, 19.2, 19.3, 19.4, 19.5, 19.6, 19.7, 19.8, 19.9, 20.0, 20.1, 2 0.2, 20.3, 20.4, 20.5, 20.6, 20.7, 20.8, 20.9, 21.0, 21.1, 21.2, 21.3, 21.4, 21.5, 21.6, 21.7, 21.8, 21.9, 22.0, 22.1, 22.2, 22.3, 22.4, 22.5, 22.6, 2 2.7, 22.8, 22.9, 23.0, 23.1, 23.2, 23.3, 23.4, 23.5, 23.6, 23.7, 23.8, 23.9, 24.0, 24.1, 24.2, 24.3, 24.4, 24.5, 24.6, 24.7, 24.8, 24.9, 25.0 mg, or a combination thereof any range defined by both and any values and subranges subsumed within that range;

Vitamin C: 105-900 mg, preferably 185-650 mg, eg 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195 , 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380, 385, 390, 395, 400, 405, 410, 415, 420, 425, 430, 435, 440, 445, 450, 455, 460, 465, 470, 475, 480, 485, 490, 495, 500, 505, 510, 515, 520, 525, 530, 535, 540, 545, 550, 555, 560, 565, 570, 575, 580, 585, 590, 595, 600, 605, 610, 615, 620, 625, 630, 635, 640, 645, 650, 655, 660, 665, 670, 675, 685, 690, 695, 700, 705, 710, 715, 720, 725, 730, 735, 740, 745, 750, 755, 760, 765, 770, 775, 780, 785, 790, 795, 800, 805, 810, 815, 820, 825, 830, 835, 840, 845, 850, 855, 860, 865, 870, 875, 880, 885, 890, 895, 900 mg, or a range defined by any two thereof and any values and subranges subsumed within that range; and

Biotin: 12.0-130.0 μg, preferably 21.0-86.8 μg, such as 12.0, 13.0, 14.0, 15.0, 16.0, 17.0, 17.5, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0 ,29.0,30.0,31.0,32.0,33.0,34.0,35.0,36.0,37.0,38.0,39.0,40.0,41.0,42.0,43.0,44.0,45.0,46.0,47.0,48.0,49.0,50.0,51.0,52.0, 53.0 ,54.0,55.0,56.0,57.0,58.0,59.0,60.0,61.0,62.0,63.0,64.0,65.0,66.0,67.0,68.0,69.0,70.0,71.0,72.0,73.0,74.0,75.0,76.0,77.0, 78.0 . .0, 103.0 ,104.0,105.0,106.0,107.0,108.0,109.0,110.0,111.0,112.0,113.0,114.0,115.0,116.0,117.0,118.0,119.0,120.0,121.0,122.0,123.0, 124.0, 125.0, 126.0, 127.0, 128.0 , 129.0, 130.0 μg, or a range defined by any two thereof and any values and subranges subsumed within that range.

In a preferred embodiment, each gram of compound vitamin composition in the nutritional composition comprises the following components: vitamin A: 1080-3080 μg RE, vitamin E: 18-49 mg α-TE, vitamin _B1 : 1.9-4.8 mg, Vitamin D: 36.6-91.0μg, Vitamin _K1 : 138-448μg, Vitamin _B2 : 890-2600μg, Vitamin _B6 : 0.8-3.4mg, Vitamin _B12 : 3.6-11.0μg, Niacin: 8.0-26.0 mg, folic acid: 180-790μg, pantothenic acid: 6.0-17.6mg, vitamin C: 185-650mg, biotin: 21.0-86.8μg.

When the nutritional composition of the present invention further includes one of vitamin D, vitamin K ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , niacin, folic acid, pantothenic acid, vitamin C, and biotin or more, especially all of them, it can more balance the nutrition needed by the human body, and better promote the improvement of the body's immune system, enhance the body's immunity, reduce the risk of various diseases, etc., and at the same time promote the nutrition components Digestion and absorption.

Compound Minerals

The mineral complex contains iron and zinc.

A part or all of iron is derived from ferrous gluconate, for example, iron is derived from ferrous gluconate or a combination of ferrous gluconate and one or more of ferrous fumarate, ferric citrate, and ferric pyrophosphate, such as All derived from ferrous gluconate.

Preferably, relative to 1 mass part of calcium citrate, the amount of iron element can be 0.0005-0.15, preferably 0.001-0.10, preferably 0.005-0.02, for example, can be 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.010, 0.015, 0.020, 0.025, 0.030, 0.035, 0.040, 0.045, 0.050, 0.055, 0.060, 0 .065, 0.070, 0.075, 0.080, 0.085, Ranges defined by 0.090, 0.095, 0.10, 0.11, 0.12, 0.13, 0.14, 0.15, or any two thereof and any values and subranges subsumed within that range.

Some or all of the zinc is derived from zinc gluconate and/or zinc citrate, for example all is derived from zinc gluconate and/or zinc citrate, such as zinc is derived from zinc gluconate, zinc citrate, or zinc citrate and gluconic acid Zinc, such as zinc derived from zinc citrate, or zinc citrate and zinc gluconate. For example, in one embodiment, the compound minerals include zinc citrate and zinc gluconate, wherein the mass ratio of zinc derived from zinc citrate to zinc derived from zinc gluconate can be (0.1-10) :1, preferably (0.5-2):1, such as 0.1:1, 0.2:1, 0.25:1, 0.3:1, 0.4:1, 0.5:1, 0.6:1, 0.7:1, 0.8:1 , 0.9:1, 1.0:1, 2.0:1, 2.5:1, 3.0:1, 4.0:1, 5.0:1, 6.0:1, 7.0:1, 8.0:1, 9.0:1, 10.0:1, or A range defined by any two thereof and any values and subranges subsumed within that range.

Preferably, relative to 1 mass part of calcium citrate, the amount of zinc element can be 0.0005-0.10 in terms of mass parts, preferably 0.001-0.05, preferably 0.005-0.02, for example can be 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.0010,0.0015,0.0020,0.0025,0.0030,0.0035,0.0040,0.0045,0.0050,0.0055,0.0060,0.0065,0.0070,0.0075,0.0080,0.0085,0.0090,0. 0095, 0.010, 0.011, 0.012, 0.013, 0.014, 0.015, 0.016, 0.017, 0.018, 0.019, 0.020, 0.021, 0.022, 0.023, 0.024, 0.025, 0.026, 0.027, 0.028, 0.029, 0.030, 0.031, 0.032, 0.033, 0.034, 0.035, 0.036, 0.037, 0.038, 0.039, 0.040, 0.041, 0.042, 0.043, 0.044, 0.045, 0.046, 0.047, 0 .048, 0.049, 0.050, 0.051, 0.052, 0.053, 0.054, 0.055, 0.056, 0.057, 0.058, 0.059, 0.060, 0.061, 0.062, 0.063, 0.064, 0.065, 0.066, 0.067, 0.068, 0.069, 0.070, 0.071, 0.072, 0 .073, 0.074, 0.075, 0.076, 0.077, 0.078, 0.079, 0.080, 0.081, 0.082, 0.083, 0.084, 0.085, 0.086, 0.087, 0.088, 0.089, 0.090, 0.091, 0.092, 0.093, 0.094, 0.095, 0.096, 0.097, 0 .098, 0.099, 0.10, or any of these Both defined ranges and any values and subranges subsumed within that range.

The inventors found that when a part or all of the iron is especially all derived from ferrous gluconate, a part or all of the zinc is especially all derived from zinc gluconate and/or zinc citrate, and the amount of iron and zinc elements is within the above-mentioned Within the range, the nutritional composition of the present invention can better improve the immune system of the body, enhance the immunity of the body, reduce the risk of various diseases, etc., and at the same time promote the digestion and absorption of nutritional components.

In addition, the present inventors have also found that in the nutritional composition of the present invention, when zinc citrate is used to partially or completely replace zinc gluconate, for example, it can enhance the body's immunity, reduce the risk of various diseases, and at the same time promote digestion. Better results can be achieved in terms of absorption. Furthermore, the inventors also found that when zinc citrate and zinc gluconate are used in combination, there is a synergistic effect between the two, and the effect can be improved synergistically.

In addition to the defined sources of iron (ferrous gluconate) and zinc (zinc gluconate and/or zinc citrate), the complex minerals may optionally further comprise iron from other sources commonly used in the art (such as ferrous sulfate) and zinc (such as zinc sulfate).

In addition to iron and zinc, the compound minerals may further include one or more of the following elements: magnesium, manganese, iodine, copper, selenium. For example, in one embodiment, the mineral complex may further include magnesium, manganese, iodine, copper, and selenium.

In one embodiment, the mineral complex may further comprise magnesium.

Preferably, part or all of the magnesium is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate.

Preferably, when present, relative to 1 mass part of calcium citrate, the amount of magnesium element can be 0.001-0.2, preferably 0.005-0.1, preferably 0.01-0.05, such as 0.001, 0.0015, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.010, 0.015, 0.020, 0.025, 0.030, 0.035, 0.040, 0.045, 0.050, 0.055, 0.060, 0.065, 0.070, 0.075, 0.080, 0.085, 0.090, 0.095, 0.10, 0.11, 0.12, 0.13, 0.14, 0.15, 0 .16, 0.17, 0.18, 0.19, 0.20, or a range defined by any two thereof, and any values and subranges subsumed within that range.

In one embodiment, the complex minerals may further comprise manganese.

Preferably, part or all of the manganese is derived from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all from magnesium sulphate .

Preferably, when present, with respect to 1 mass part of calcium citrate, the amount of manganese element can be 0.000006-0.0006, preferably 0.00001-0.0004, preferably 0.00005-0.0002, such as 0.000006, 0.000007, 0.000008, 0.000009, 0.00001, 0.00002, 0.00003, 0.00004, 0.00005, 0.00006, 0.00007, 0.00008, 0.00009, 0.00010, 0.00015, 0.00020, 0.00025, 0.00030, 0.00035, 0.00040, 0.00045, 0.00050, 0.00055, 0.00060, or any two of them range and any values and subranges subsumed within that range.

In one embodiment, the complex minerals may further comprise iodine.

Preferably, part or all of iodine is derived from potassium iodate, for example all is derived from potassium iodate.

Preferably, when present, relative to 1 mass part of calcium citrate, the amount of iodine element can be 0.000003-0.0015, preferably 0.00005-0.001, preferably 0.0001-0.0005, such as 0.000003, 0.000004, 0.000005, 0.000006, 0.000007, 0.000008, 0.000009, 0.000010, 0.000015, 0.000020, 0.000025, 0.000030, 0.000035, 0.000040, 0.000045, 0.000050, 0.00 0055, 0.000060, 0.000065, 0.000070, 0.000075, 0.000080, 0.000085, 0.000090, 0.000095, 0.00010, 0.00015, 0.00020, 0.00025, 0.00030, 0.00035, 0.00040, 0.00045, 0.00050, 0.00055, 0.00060, 0.00065, 0.00070, 0.00075, 0.00080, 0.00085, 0.00090, 0.00095, 0 .0010, 0.0011, 0.0012, 0.0013, 0.0014, 0.0015, or any combination thereof range and any values and subranges subsumed within that range.

In one embodiment, the complex minerals may further comprise copper.

Preferably, part or all of the copper is derived from copper sulfate and/or copper citrate and/or copper gluconate, for example entirely derived from copper sulfate and/or copper citrate and/or copper gluconate, for example entirely derived from copper sulfate .

Preferably, when present, relative to 1 mass part of calcium citrate, the amount of copper element can be 0.00004-0.006, preferably 0.0001-0.005, preferably 0.0005-0.002, such as 0.00004, 0.00005, 0.00006, 0.00007, 0.00008, 0.00009, 0.00010, 0.00015, 0.00020, 0.00025, 0.00030, 0.00035, 0.00040, 0.00045, 0.00050, 0.00055, 0.00060, 0.00065, 0 .00070, 0.00075, 0.00080, 0.00085, 0.00090, 0.00095, 0.0010, 0.0015, 0.0020, 0.0025, 0.0030, 0.0035, 0.0040, 0.0045, 0.0050, 0.0055, 0.0060, or a range defined by any two thereof and any values and subranges subsumed within that range.

In one embodiment, the complex minerals may further comprise selenium.

Preferably, part or all of the selenium is derived from sodium selenite, for example all of it is derived from sodium selenite.

Preferably, when present, relative to 1 mass part of calcium citrate, the amount of selenium in terms of parts by mass can be 0.000001-0.0002, preferably 0.000002-0.0002, preferably 0.00001-0.0001, for example can be 0.000001, 0.000002, 0.000003, 0.000004, 0.000005, 0.000006, 0.000007, 0.000008, 0.000009, 0.000010, 0.000015, 0.000020, 0.000025, 0.000030, 0.000035, 0.000040, 0.00 0045, 0.000050, 0.000055, 0.000060, 0.000065, 0.000070, 0.000075, 0.000080, 0.000085, 0.000090, 0.000095, 0.00010, Ranges defined by 0.00011, 0.00012, 0.00013, 0.00014, 0.00015, 0.00016, 0.00017, 0.00018, 0.00019, 0.00020, or any two thereof and any values and subranges subsumed within that range.

The compound minerals may optionally further contain other mineral components, such as calcium carbonate, sodium citrate, potassium chloride, calcium hydrogen phosphate and the like.

In one embodiment, the compound minerals further include auxiliary materials.

The adjuncts are present in amounts to aid in the effective dispersion of the mineral components mentioned. Preferably, when present, relative to 1 mass part of calcium citrate, the amount of the auxiliary material contained in the compound mineral may be 0.01-10 mass parts, such as 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, 0.40, 0.45, 0.50, 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85, 0.90, 0.95, 1.0, 1.5, 2.0 , 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, or the range defined by any two thereof and any value included in the range and subranges.

In a preferred embodiment, the compound minerals include the following:

Iron, part or all of which is derived from ferrous gluconate, preferably derived from ferrous gluconate or a combination of ferrous gluconate and one or more of ferrous fumarate, ferric citrate, and ferric pyrophosphate , preferably all of which are derived from ferrous gluconate;

Zinc, part or all of which is derived from zinc citrate and/or zinc gluconate, preferably entirely derived from zinc citrate and/or zinc gluconate;

Magnesium, preferably derived from magnesium sulfate and/or magnesium gluconate;

Manganese, preferably derived from manganese sulfate and/or manganese citrate and/or manganese gluconate;

Iodine, preferably derived from potassium iodate;

Copper, preferably derived from copper sulfate and/or copper citrate and/or copper gluconate; and

Selenium, preferably derived from sodium selenite.

In a further preferred embodiment, the compound minerals include the following:

Iron, all derived from ferrous gluconate;

Zinc, all derived from zinc citrate and/or zinc gluconate;

Magnesium, derived from magnesium sulfate and/or magnesium gluconate;

Manganese, derived from manganese sulfate and/or manganese citrate and/or manganese gluconate;

Iodine, which is derived from potassium iodate;

Copper, derived from copper sulfate and/or copper citrate and/or copper gluconate; and

Selenium, which is derived from sodium selenite.

In one embodiment, in the nutritional composition, each gram of compound minerals includes the following elements:

Iron: 6.0-89.0 mg, preferably 12.0-59.5 mg, eg 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0, 29.0, 30.0, 31.0, 32.0, 33.0, 34.0, 35.0, 36.0, 37.0, 38.0, 39.0, 40.0, 41.0, 42.0, 43.0, 44.0, 45.0, 46.0, 47.0, 4 8.0, 49.0, 50.0, 51.0, 52.0, 53.0, 54.0, 55.0, 56.0, 57.0, 58.0, 59.0, 60.0, 61.0, 62.0, 63.0, 64.0, 65.0, 66.0, 67.0, 68.0, 69.0, 70.0, 71.0, 72.0, 7 3.0, 74.0, 75.0, 76.0, 77.0, 78.0, 79.0, 80.0, 81.0, 82.0, 83.0, 84.0, 85.0, 86.0, 87.0, 88.0, 89.0 mg, or the range defined by any two thereof and any values and subranges;

Zinc: 3.5-75.0 mg, preferably 7.0-55.2 mg, eg 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0, 29.0, 30.0, 31.0, 32.0, 33.0, 34.0, 35.0, 36.0, 37.0, 38.0, 39.0, 40.0, 41.0, 42.0, 4 3.0, 44.0, 45.0, 46.0, 47.0, 48.0, 49.0, 50.0, 51.0, 52.0, 53.0, 54.0, 55.0, 56.0, 57.0, 58.0, 59.0, 60.0, 61.0, 62.0, 63.0, 64.0, 65.0, 66.0, 67.0, 6 8.0, 69.0, 70.0, 71.0, 72.0, 73.0, 74.0, 75.0 mg, or a range defined by any two thereof and any values and subranges subsumed within that range;

Magnesium: 10.0-120.0 mg, preferably 19.8-79.0 mg, eg 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 16.5, 17.0, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0, 29.0, 30.0, 31.0, 32.0, 33.0, 34.0, 35.0, 36.0, 37.0, 38.0, 39.0, 40.0, 41.0, 42.0, 43.0, 44.0, 45.0, 46.0, 47.0, 48.0, 49.0, 50.0, 5 1.0, 52.0, 53.0, 54.0, 55.0, 56.0, 57.0, 58.0, 59.0, 60.0, 61.0, 62.0, 63.0, 64.0, 65.0, 66.0, 67.0, 68.0, 69.0, 70.0, 71.0, 72.0, 73.0, 74.0, 75.0, 7 6.0, 77.0, 78.0, 79.0, 80.0, 81.0, 82.0, 83.0, 84.0, 85.0, 86.0, 87.0, 88.0, 89.0, 90.0, 91.0, 92.0, 93.0, 94.0, 95.0, 96.0, 97.0, 98.0, 99.0, 100.0, 101.0, or limited by any two of them range and include Any value within that range and subranges;

Manganese: 45-400 μg, preferably 84-294 μg, such as 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380, 385, 390, 395, 400 μg, or a range defined by any two thereof and any values and subranges subsumed within that range;

Iodine: 20-900 μg, preferably 40-560 μg, for example 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 345, 350, 355, 360, 365, 370, 375, 380, 385, 390, 395, 400, 405, 410, 415, 420, 425, 430, 435, 440, 445, 450, 455, 460, 465, 470, 475, 480, 485, 490, 495, 500, 505, 510, 515, 520, 525, 530, 535, 540, 545, 550, 555, 560, 565, 570, 575, 580, 585, 590, 595, 600, 605, 610, 615, 620, 625, 630, 635, 640, 645, 650, 655, 660, 665, 670, 675, 680, 685, 690, 695, 700, 705, 710, 715, 720, 725, 730, 735, 740, 745, 750, 755, 760, 765, 770, 775, 780, 785, 790, 795, 800, 805, 810, 815, 820, 825, 830, 835, 840, 845, 850, 855, 860, 865, 870, 875, 880, 885, 890, 895, 900 μg, or the range defined by any two thereof and any value and subrange subsumed within that range;

Copper: 300-4200 μg, preferably 600-2800 μg, for example 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500, 1550, 1600, 1650, 1700, 1750, 1800, 1850, 1900, 1950, 2000, 2050, 2100, 2150, 2200, 2250, 2300, 2350, 2400, 2 450、 2500, 2550, 2600, 2650, 2700, 2750, 2800, 2850, 2900, 2950, 3000, 3050, 3100, 3150, 3200, 3250, 3300, 3350, 3400, 3450, 3500, 3550, 3600, 3650, 3 700, 3750, 3800, 3850, 3900, 3950, 4000, 4050, 4100, 4150, 4200 μg, or ranges defined by any two thereof and any values and subranges subsumed within such ranges;

Selenium: 10-130 μg, preferably 24-98 μg, for example 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130 μg, or a range defined by any two thereof and any value and subrange subsumed within that range.

In a preferred embodiment, in the nutritional composition, each gram of compound minerals includes the following elements: iron: 12.0-59.5mg, zinc: 7.0-55.2mg, magnesium: 19.8-79.0mg, manganese: 84- 294μg, iodine: 40-560μg, copper: 600-2800μg, selenium: 24-98μg.

When the compound minerals further include magnesium, manganese, iodine, copper, selenium in addition to a part or all of iron derived from ferrous gluconate and a part or all of zinc derived from zinc gluconate and/or zinc citrate One or more of them, especially all of them, can more balance the nutrition needed by the human body, better promote the improvement of the body's immune system, enhance the body's immunity, reduce the risk of various diseases, etc., and at the same time can promote nutrition. Partial digestion and absorption.

In the nutritional composition of the present invention, relative to 1 mass part of calcium citrate, the amount of compound vitamins can be 0.15-1.33, preferably 0.2-1.1, such as 0.15, 0.16, 0.17, 0.18, 0.19, 0.20, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.30, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.40, 0.41, 0.42, 0.43, 0 .44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.50, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.60, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0 .69, 0.70, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.80, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.90, 0.91, 0.92, 0.93, 0 .94, 0.95, 0.96, 0.97, 0.98, 0.99, 1.00, 1.01, 1.02, 1.03, 1.04, 1.05, 1.06, 1.07, 1.08, 1.09, 1.10, 1.11, 1.12, 1.13, 1.14, 1.15, 1.16, 1.17, 1.18, 1 .19, 1.20, 1.21, 1.22, 1.23, 1.24, 1.25, 1.26, 1.27, 1.28, 1.29, 1.30, 1.31, 1.32, 1.33, or a range defined by any two thereof and any values and subranges subsumed within that range.

In the nutritional composition of the present invention, relative to 1 mass part of calcium citrate, the amount of compound minerals can be 0.15-1.33 in terms of mass parts, preferably 0.2-1.1, such as 0.15, 0.16, 0.17, 0.18, 0.19 , 0.20, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.30, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.40, 0.41, 0.42, 0.43, 0.44 , 0.45, 0.46, 0.47, 0.48, 0.49, 0.50, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.60, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69 , 0.70, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.80, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.90, 0.91, 0.92, 0.93, 0.94 , 0.95, 0.96, 0.97, 0.98, 0.99, 1.00, 1.01, 1.02, 1.03, 1.04, 1.05, 1.06, 1.07, 1.08, 1.09, 1.10, 1.11, 1.12, 1.13, 1.14, 1.15, 1.16, 1.17, 1.18, 1.19 , 1.20, 1.21, 1.22, 1.23, 1.24, 1.25, 1.26, 1.27, 1.28, 1.29, 1.30, 1.31, 1.32, 1.33, or a range defined by any two thereof and any values and subranges subsumed within that range.

For example, in one embodiment, in the nutritional composition, the mass ratio of compound vitamins: compound minerals: calcium citrate may be (0.15-1.33): (0.15-1.33): 1, preferably ( 0.2-1.1):(0.2-1.1):1.

In an exemplary nutritional composition of the present invention,

Each g of compound vitamins contains the following ingredients: vitamin A (retinyl palmitate): 900-3300μg RE, vitamin E (d-α-tocopheryl acetate): 15-52.5mg α-TE, vitamin _B1 (sulfur hydrochloride amine): 1.6-5.1mg, vitamin D (cholecalciferol): 30.5-97.5μg, vitamin K ₁ (phytonadione): 115-480μg, vitamin B ₂ (riboflavin): 850-2700μg, vitamin B ₆ (pyridoxine hydrochloride): 0.7-3.6mg, vitamin B ₁₂ (cyanocobalamin): 3-12μg, niacin (nicotinamide): 7-27mg, folic acid (folic acid): 150-825μg, pantothenic acid (D - calcium pantothenate): 5.5-18 mg, vitamin C (sodium L-ascorbate): 175-675 mg, biotin (D-biotin): 17.5-93 μg, taurine (taurine): 85-270 mg, balance as an excipient (lactose);

Each gram of compound minerals contains the following ingredients: iron (ferrous gluconate): 10-63.75mg, zinc (zinc citrate): 6-27mg, magnesium (magnesium sulfate): 16.5-79mg, manganese (manganese sulfate): 70 -315μg, iodine (potassium iodate): 33.5-600μg, copper (copper sulfate): 500-3000μg, selenium (sodium selenite): 20-105μg, and the balance is excipients (maltodextrin).

The mass ratio of compound vitamins: compound minerals: calcium citrate is 0.15-1.33:0.15-1.33:1.

formula food

The nutritional composition of the present invention can be added to formula foods such as infant formula (such as infant formula milk powder), children's formula modified milk or milk powder, or adult formula modified milk or milk powder to prepare specific functions (such as Compared with existing formula foods such as formula foods for infants and young children, the present invention has obvious advantages and makes up for the nutritional composition of the market in this respect. points of demand.

Therefore, in another aspect, the present invention relates to a formula comprising a nutritional composition according to any embodiment of the present invention.

In one embodiment, the formula is an infant formula.

In one embodiment, the formula food is modulated formula milk or milk powder for children.

In one embodiment, the formula food is adult formula modulated milk or milk powder.

In a further embodiment, the infant formula is infant formula milk powder.

In one embodiment, in the formula food such as infant formula food, such as infant formula milk powder, children's formula modified milk or milk powder, adult formula modified milk or milk powder, the nutritional composition in terms of mass Usage amount (addition amount) can be 0.6-1.8%, preferably 0.7-1.4%, such as 0.60%, 0.65%, 0.70%, 0.75%, 0.80%, 0.85%, 0.90%, 0.95%, 1.0%, 1.05%, 1.10%, 1.15%, 1.20%, 1.25%, 1.30%, 1.35%, 1.40%, 1.45%, 1.50%, 1.55%, 1.60%, A range defined by 1.65%, 1.70%, 1.75%, 1.80%, or any two thereof and any values and subranges subsumed within that range.

In one embodiment, in the formula food such as infant formula food, such as infant formula milk powder, children formula modified milk or milk powder, adult formula modified milk or milk powder, the compound vitamin (derived from the The mass content of the nutritional composition) can be about 0.1-1%, preferably about 0.3-0.5%, such as 0.10%, 0.15%, 0.20%, 0.25%, 0.30%, 0.35%, 0.40%, 0.45%, 0.50% , 0.55%, 0.60%, 0.65%, 0.70%, 0.75%, 0.80%, 0.85%, 0.90%, 0.95%, 1.0%, or the range defined by any two thereof and any value and sub- scope.

In one embodiment, in the formula food such as infant formula food, such as infant formula milk powder, children's formula modified milk or milk powder, adult formula modified milk or milk powder, the compound mineral (derived from The mass content of the nutritional composition) can be about 0.1-1%, preferably about 0.3-0.5%, such as 0.10%, 0.15%, 0.20%, 0.25%, 0.30%, 0.35%, 0.40%, 0.45%, 0.50 %, 0.55%, 0.60%, 0.65%, 0.70%, 0.75%, 0.80%, 0.85%, 0.90%, 0.95%, 1.0%, or the range defined by any two thereof and any value included in the range and subranges.

When the nutritional composition is added to formula food such as infant formula food, such as infant formula milk powder, children's formula modified milk or milk powder, adult formula modified milk or milk powder, the nutritional composition can balance the human body's needs. All aspects of nutrition, while enhancing the body's immunity, and promoting the digestion and absorption of nutritional components.

use

In another aspect, the present invention also relates to the above-mentioned nutritional composition or the above-mentioned food in enhancing the body's immunity, promoting digestion and absorption (or improving the bioavailability of trace elements), especially vitamin A, vitamin E and vitamin _B1 and iron , zinc and calcium digestion and absorption for non-therapeutic purposes.

Example

In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

The "parts" in the following application examples are all parts by weight, and the % contents of ingredients are all % by weight.

In addition, in the following application examples, unless otherwise specified, the following sources of raw materials used as follows.

Raw milk: Heilongjiang Feihe Dairy Co., Ltd.

Skimmed milk powder: Kerry, Ireland

Concentrated Whey Protein Powder: Danish Allah

Choline chloride (choline content 72.5%): Beijing Jinkangpu Food Technology Co., Ltd.

Desalted whey powder: France Euroserum (Red Bird)

Galactooligosaccharide: Baolingbao Biological Co., Ltd.

Mixed vegetable oil (containing 1,3-dioleic acid 2-palmitic acid triglyceride): Bangelodes (Xiamen) Oil Technology Co., Ltd.

Fructo-oligosaccharide: Baolingbao Biological Co., Ltd.

Calcium citrate: JUNGBUNZLAUER, Germany

Vitamins, Minerals, Taurine: DSM

Potassium chloride: Jiangsu Province Diligent Pharmaceutical Co., Ltd.

Sodium citrate: Rizhao Jinhe Biochemical Group Co., Ltd.

Example 1

A nutritional composition for infant formula, consisting of compound vitamins, compound minerals and calcium citrate, the ratio of compound vitamins: compound minerals: calcium citrate is 0.5:0.5:1, the present invention The usage amount of the nutritional composition in the infant formula is 1.2% by weight.

Wherein: each gram of compound vitamin composition includes the following components: vitamin A: 2000 μg RE, vitamin D: 60 μg, vitamin E: 40 mg α-TE, vitamin _K1 : 350 μg, vitamin _B1 : 3.5 mg, vitamin _B2 : 1600μg, vitamin _B6 : 2.5mg, vitamin _B12 : 8μg, niacin: 17mg, folic acid: 530μg, pantothenic acid: 11mg, vitamin C: 500mg, biotin: 60μg, taurine 180mg, and the rest is lactose.

Wherein: each gram of the compound mineral composition includes the following elements: magnesium: 60mg, iron: 20mg, zinc: 18mg, manganese: 200μg, iodine: 420μg, copper: 1500μg, selenium: 66μg, and the rest is lactose.

The compound sources of the vitamins are: vitamin A is derived from retinyl palmitate, vitamin D is derived from cholecalciferol, vitamin E is derived from d-alpha-tocopheryl acetate, vitamin _K1 is derived from phytonadione, vitamin B ₁ is derived from thiamine hydrochloride, vitamin B ₂ is derived from riboflavin, vitamin B ₆ is derived from pyridoxine hydrochloride, vitamin B ₁₂ is derived from cyanocobalamin, niacin is derived from niacinamide, folic acid is derived from folic acid (pteroyl glutamic acid), pantothenic acid from D-calcium pantothenate, vitamin C from L-sodium ascorbate, and biotin from D-biotin.

The compound sources of the mineral elements are: magnesium is derived from magnesium sulfate, iron is derived from ferrous gluconate, zinc is derived from zinc citrate, manganese is derived from manganese sulfate, copper is derived from copper sulfate, iodine is derived from potassium iodate, selenium Derived from sodium selenite.

Example 2

A nutritional composition for formula food for older infants, consisting of compound vitamins, compound minerals and calcium citrate, the ratio of compound vitamins: compound minerals: calcium citrate is 0.35:0.35:1, The usage amount of the nutritional composition of the present invention in formula food for older infants is 1.45% by weight.

Wherein: each gram of compound vitamin composition includes the following components: vitamin A: 2300 μg RE, vitamin D: 60 μg _, vitamin E: 40 mg α-TE, vitamin _K1 : 250 μg, vitamin B1: 3.3 mg, vitamin _B2 : 1700μg, vitamin _B6 : 2.0mg, vitamin _B12 : 7μg, niacin: 15mg, folic acid: 400μg, pantothenic acid: 11mg, vitamin C: 400mg, biotin: 35μg, taurine 180mg, and the rest is lactose.

Wherein: each gram of the compound mineral composition includes the following elements: magnesium: 50mg, iron: 26mg, zinc: 13.5mg, manganese: 150μg, iodine: 220μg, copper: 1200μg, selenium: 40μg, and the rest is lactose.

Example 3

A nutritional composition for formula food for infants, consisting of compound vitamins, compound minerals and calcium citrate, the ratio of compound vitamins: compound minerals: calcium citrate is 0.35:0.35:1, the present invention The usage amount of the nutritional composition in the formula food for older infants is 1.45% by weight.

Wherein: _each gram of compound vitamin composition includes the following components: vitamin A: 2300 μg RE, vitamin D: 60 μg, vitamin E: 40 mg α-TE, vitamin _K1 : 250 μg, vitamin B1: 3.3 mg, vitamin _B2 : 1700μg, vitamin _B6 : 2.0mg, vitamin _B12 : 7μg, niacin: 15mg, folic acid: 400μg, pantothenic acid: 11mg, vitamin C: 400mg, biotin: 35μg, Taurine 180mg, the rest is lactose.

Wherein: each gram of the compound mineral composition includes the following elements: magnesium: 35mg, iron: 28mg, zinc: 12mg, manganese: 150μg, iodine: 80μg, copper: 1300μg, selenium: 50μg, and the rest is lactose.

Example 4

A nutritional composition for children's formula food, which consists of compound vitamins, compound minerals and calcium citrate, the ratio of compound vitamins: compound minerals: calcium citrate is 0.2:0.2:1, the present invention The usage amount of the nutritional composition in the infant formula is 1.4% by weight.

Wherein: each gram of compound vitamin composition includes the following components: vitamin A: 2000 μg RE, vitamin D: 45 μg, vitamin E: 35 mg α-TE, vitamin _B1 : 4.0 mg, vitamin C: 350 mg, and the rest is lactose.

Wherein: each gram of the compound mineral composition comprises the following elements: magnesium: 20mg, iron: 46mg, zinc: 50mg, and the rest is lactose.

The source of the vitamin compound is: vitamin A is derived from retinyl palmitate, vitamin D is derived from cholecalciferol, vitamin E is derived from d-α-tocopheryl acetate, vitamin B1 is derived from thiamine hydrochloride, and vitamin C is derived from In sodium L-ascorbate, biotin is derived from D-biotin.

The compound sources of the mineral elements are: magnesium is derived from magnesium sulfate, iron is derived from ferrous gluconate, zinc is derived from zinc citrate and zinc gluconate, wherein zinc derived from zinc citrate and zinc derived from zinc gluconate The zinc ratio is 1:1.

Application example 1:

An application of the nutritional composition of infant formula food in infant formula milk powder, every 1000 parts of infant formula milk powder is made of the following components by weight:

The used raw materials of the milk powder of the present invention include: 200 parts of raw milk (on a dry basis), 30 parts of concentrated whey protein powder, 400 parts of desalted whey powder, 127.5 parts of lactose, 25 parts of fructooligosaccharides, 200 parts of mixed vegetable oil, 1.5 parts of choline chloride, 2 parts of sodium citrate, 2 parts of potassium chloride, 12 parts of nutritional composition of embodiment 1 (percentage by weight 1.2%); Concentrate and spray-dry to form a powdery semi-finished product, and carry out nitrogen-filled packaging to obtain the final product.

Application example 2:

An application of a nutritional composition of formula food for older infants in infant formula milk powder, wherein every 1000 parts of formula milk powder for older infants is prepared from the following components by weight:

The raw materials used in the milk powder of the present invention include: 200 parts of raw milk (on a dry basis), 30 parts of concentrated whey protein powder, 250 parts of desalted whey powder, 75 parts of skimmed milk powder, 250 parts of lactose, and 25 parts of fructooligosaccharides , 150 parts of mixed vegetable oil, 1.5 parts of choline chloride, 2 parts of sodium citrate, 2 parts of potassium chloride, 14.5 parts of the nutritional composition of Example 2 (1.45% by weight); , pasteurization, evaporative concentration and spray drying into a powdery semi-finished product, which is packed with nitrogen to obtain the final product.

Application example 3:

An application of a nutritional composition of infant formula food in infant formula milk powder, every 1000 parts of infant formula milk powder is made of the following components by weight:

The raw materials used in the milk powder of the present invention include: 260 parts of raw milk (on a dry basis), 20 parts of concentrated whey protein powder, 250 parts of desalted whey powder, 125 parts of skimmed milk powder, 175 parts of lactose, and 25 parts of fructooligosaccharides , 125 parts of mixed vegetable oil, 1.5 parts of choline chloride, 2 parts of sodium citrate, 2 parts of potassium chloride, 14.5 parts of nutritional composition of embodiment 3 (1.45% by weight); , pasteurization, evaporative concentration and spray drying into a powdery semi-finished product, which is packed with nitrogen to obtain the final product.

Application example 4:

A kind of application of the nutritional composition of children's formula food in children's formula milk powder, every 1000 parts of children's formula milk powder are made of the following components by weight:

The raw materials used in the milk powder of the present invention include: 360 parts of raw milk (on a dry basis), 30 parts of concentrated whey protein powder, 300 parts of desalted whey powder, 150 parts of skimmed milk powder, 50 parts of maltodextrin, fructooligosaccharide 30 parts, 60 parts of mixed vegetable oil, 14 parts of nutritional composition of embodiment 4 (1.4% by weight); Nitrogen packaging is the final product.

Animal experiment example

Animal experiments mainly verify the differences in immune function and digestion and absorption rate between the preferred composition of vitamin A, vitamin E, vitamin B ₁ , calcium, iron and zinc in this program and the original composition, and enhance the immune function of the preferred components. Evaluate the bioavailability of energy and trace elements, and conduct animal experiments to verify it, and provide support for product development.

In this study, primary weaned rats were selected and fed with nutrient packs (containing vitamins A, E, B1, zinc, iron, and calcium in different compound forms and doses) for 5 weeks, and the contents of the above six nutrients in the body were compared and analyzed. Evaluate the effects of different nutrient packages on the immunity, growth and development of rats in each group. Through the implementation of this project, it is possible to screen out the combination of compound nutritional fortifiers whose ingredients are close to breast milk or whose bioavailability is relatively high, which will be alienated from the existing compound nutritional fortifiers in the market. Through animal experiments, it is verified that differentiated nutrients have certain advantages in certain physiological functions, and provide a scientific basis for screening the optimal nutrient formula combination.

1 Materials and methods

1.1 Main reagent consumables

BCA method protein content determination kit; rat interleukin 2 (IL-2) ELISA kit, rat interleukin 4 (IL-4) ELISA kit, rat interferon gamma (IFN-γ) ELISA kit, Rat Immunoglobulin A (IgA) ELISA Kit, Rat Immunoglobulin G (IgG) ELISA Kit, Rat Immunoglobulin M (IgM) ELISA Kit. Nitric acid (65%) was purchased from Merck, Germany, high-purity water was provided by Milli-Q Advantage ultrapure water system, acetonitrile and methanol (Fisher, USA).

1.2 Main instruments and equipment

Microplate reader: Labsystems Multiskan MS (Finland); electronic balance: BS-110S, Beijing Sartorius Balance Co., Ltd.; TG16W micro high-speed centrifuge; desktop centrifuge: type 5424R, Eppendorf (Germany); electronic balance: Mettler XS105DU (Switzerland); AC8 plate washer: Thermo Labsystems (Finland); GNP-9080 water-proof constant temperature incubator; vortex instrument, water bath, tissue homogenizer.

Atomic absorption spectrometer: PinA Acle 900T, Perkin Elmer Company; UPLC chromatograph: Accucore Vanquish series (including high-pressure binary system pump, automatic sampler, diode array detector, fluorescence detector, American Thermo Fisher Scientific Co., Ltd. .Waters Alliance 2695 high performance liquid chromatography system (including high-pressure binary system pump, autosampler, fluorescence detector and Empower chromatography data system, etc.).Mars classic 6 automatic high-throughput microwave digestion and extraction system (PYNN, USA Paian Company), EHD-40 adjustable electric furnace, chromatographic column: Agilent ZORBAOX SB-C18 column (250mm×4.6mm×5μm); Thermo scientific Hypersil GOLD AQ column (250mm×4.6mm×5μm).

1.3 Experimental animals

SPF primary weaned male Sprague-Dawley (SD) rats, weighing 50-70 g, were purchased from the Department of Experimental Animal Science, Peking University Health Science Center, license number SCXK (Beijing) 2016-0012. All rats were raised in an environment of 25±2°C and humidity of 55±2%, free to eat and drink, and naturally alternate day and night. The animal experiments complied with the ethical requirements of experimental animals, and after adaptive feeding, they were given custom-made feeds to start the formal experiment. Rats were divided into random groups, 8 in each group, each mouse was housed in a single cage, and fed freely.

1.4 Experimental grouping

Randomly divided into blank control group (without adding vitamins and mineral packs), the sources of vitamin A, vitamin E, vitamin B ₁ , iron and zinc in the nutritional composition of control group 1 and 2 were retinyl acetate, dl - α-tocopherol acetate, thiamine nitrate, iron pyrophosphate, zinc sulfate, the calcium used in combination is calcium carbonate; in the nutritional composition of experimental groups 1 and 2, the details of the addition of each nutrient in the control group and experimental group per 10kg of feed See Table 1 and Table 2. Wherein, the active ingredient content of each nutrient in the control group 1 and the experimental group 1, the control group 2 and the experimental group 2 is the same. The sources of vitamin A, vitamin E, vitamin B ₁ , and iron compounds in the nutritional compositions of experimental groups 3, 4, and 5 were retinyl palmitate, d-α-tocopheryl acetate, thiamine hydrochloride, and gluconate, respectively. The sources of iron and zinc are zinc gluconate, zinc citrate, zinc gluconate+zinc citrate (1:1), and the calcium used in combination is calcium citrate. The active ingredients of each nutrient have the same content. See Table 3 for the addition details.

Table 1 Addition details of each nutrient in every 10kg feed in the control group

Table 2 Addition details of each nutrient in every 10kg feed in experimental group 1-2

Table 3 Addition details of each nutrient in every 10kg feed in experimental group 3-5

1.5 Experimental method

Rat feed in each experimental group was provided by Beijing HFK Bioscience CO., LTD according to the nutrient formula of each group. Newly weaned rats were adaptively fed After breeding, they were randomly divided into groups of 8, and were given custom-made sample feeds, and the formal experiment began.

Weigh every week, and record the body weight and feed consumption of the mice.

Rats in each group took blood from the orbital venous plexus at 1, 2, 3, 4 and 5 weeks respectively, 1.2-1.5mL per rat, let stand for 30min after blood collection, centrifuged at 3000r/min for 10min, and took serum in -80℃ refrigerator Stored for the detection of immune factors IL-2, IL-4, IFN-γ and immunoglobulins IgM, IgA, IgG in rat serum.

The animal experiment was carried out until the end of the fifth week, and the rats were given anesthesia, blood was collected from the heart, left to stand for 30 minutes, 3000r/min, centrifuged for 10 minutes, and the serum was stored at -80°C for vitamin A, E and B1 in the serum, as well as immune factors Immediately after the blood was collected, the heart, liver, kidney, spleen, and femurs on both sides were dissected, and the heart, liver, kidney, and spleen were weighed and recorded. The liver and kidneys were bagged and placed in an ice box. Re-record, vernier caliper to measure the length and record the diameter at the midpoint of the femur. The measured femur was also bagged and stored in an ice box. After all the experiments were completed, it was stored in a -80°C refrigerator. The liver, kidney and bones were used for the analysis and detection of Ca, Fe, and Zn elements.

1.6 Detection indicators

1.6.1 Detection of immune factors and immunoglobulins in serum

immune factor

IL-2 is one of the earliest discovered cytokines, also known as T cell growth factor, which can promote the proliferation of T cells, induce their differentiation and apoptosis.

IL-4 is mainly secreted by activated T cells and mononuclear cells. It can stimulate the growth and proliferation of B cells, promote and regulate the production of IgE, promote the growth and proliferation of T cells, but inhibit the function of Th1 cells and other biological functions.

IFN-γ is a multifunctional active protein produced by monocytes and lymphocytes. It activates phagocytes to kill antigens and tumor cells, and strengthens neutrophils to enhance the killing activity of NK cells, so as to achieve the goal of protecting the body. The lethality of tumor cells, resulting in the apoptosis of tumor cells. Therefore, this experiment compares and evaluates the influence of the composition of the present invention on the immune function of rats by measuring the secretion levels of IL-2, IL-4 and IFN-γ in rat serum.

Blood was collected from the orbital venous plexus of the rats in each group at 1, 2, 3, 4, and 5 weeks respectively, and 1.2-1.5 mL of blood was collected from each rat. After blood collection, it was left to stand for 30 minutes, centrifuged at 3000 r/min for 10 minutes, and the serum was collected in a -80°C refrigerator. Stored, and ELISA kits were used to detect immune factors IL-2, IL-4, and IFN-γ in serum.

Immunoglobulin

Immunoglobulin refers to a globulin with antibody activity or a chemical structure similar to an antibody. Immunoglobulin is a receptor on the surface of B lymphocytes, which plays an important role in various cell activities such as cell activation and differentiation. Immunoglobulins are secreted into blood or other body fluids and can bind to the corresponding antigen, which results in direct neutralization of foreign antigens, or as an initial trigger to initiate and recruit effector systems, such as activation of complement or mononuclear phagocyte-mediated Antibody-dependent cell lysis. This experiment compares and evaluates the effect of breastmilk nutrition on the immune function of rats by measuring the secretion content of immunoglobulin IgM, IgA and IgG in rat serum.

Blood was collected from the orbital venous plexus of the rats in each group at 1, 2, 3, 4, and 5 weeks respectively, and 1.2-1.5 mL of blood was collected from each rat. After blood collection, it was left to stand for 30 minutes, centrifuged at 3000 r/min for 10 minutes, and the serum was collected in a -80°C refrigerator. Stored, and ELISA kits were used to detect the immunoglobulins IgM, IgA, and IgG in the serum.

1.6.2 Detection of vitamin A, vitamin E and _B1 in blood

Vitamin A, E serum sample processing method: thaw the frozen serum naturally at room temperature and in the dark, shake and mix. Take 1ml of rat serum, put it in a 10ml centrifuge tube, add 3ml of methanol-acetonitrile 2:1 mixed solvent, mix well, vortex for 30s, ultrasonic for 1min, stand at room temperature for 15min to precipitate protein, then add 4.5ml of n-hexane, Vortex, extract for 1min, centrifuge at 10000r for 5min, take 4ml of the supernatant organic layer and place it in a 5ml EP tube, dry it with nitrogen at room temperature, add 500ul of methanol to the residue to redissolve, ultrasonically dissolve, and perform HPLC determination.

Vitamin B ₁ sample processing method: take 500 μL of serum and place it in an EP tube, add 50 μL of 0.5 mmol/L sodium hydroxide solution, and vortex for 30 seconds. Add 5 mL of ethyl acetate, vortex for 3 min, centrifuge at 5000 r/min at 4°C for 5 min, remove 4 mL of supernatant, and blow dry with nitrogen in a water bath at 37°C. Take 200 μL of the residue for reconstitution, and take 20 μL for HPLC analysis.

1.6.3 Calcium, zinc and iron content detection in liver, kidney and bone tissue

Ca, Fe, Zn sample processing: Accurately weigh 0.3g-0.5g of liver, kidney and bone samples into a polytetrafluoroethylene digestion tube, add 8mL of nitric acid, and digest the samples according to the operation steps of microwave digestion. The digestion conditions are shown in the table 3. After cooling, take out the digestion tube and concentrate to about 1 mL on an electric furnace at 160°C. After cooling the digestion tube, transfer the digestion solution to a 10mL volumetric flask, wash the digestion tube 2 to 3 times with a small amount of water, put the washing solution in the volumetric flask and dilute to the mark with water. Dilute according to actual measurement needs, mix well for later use, and use flame atomic absorption spectrometry for detection.

Table 3 Reference conditions of microwave digestion heating program

2 results

2.1 Contents of immune factors and immunoglobulins in serum

The impact of each group of test substances on serum IL-4 content (mean ± SE, n=10, pg/ml) after 1-5 weeks in each group of table 4

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Table 5. The impact of each group of test substances on serum IFN-γ content (mean ± SE, pg/ml) for 1-5 weeks

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Table 6. Effects of each group of test substances on serum IL-2 content (mean ± SE, pg/ml) after 1-5 weeks

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Table 7. The impact of each group of test substances on serum IgA content (mean ± SE, μg/ml) after 1-5 weeks

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Table 8. Effects of each group of test substances on serum IgG content (mean ± SE, mg/ml) after 1-5 weeks

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Table 9. Effects of each group of test substances on serum IgM content (mean ± SE, μg/ml) after 1-5 weeks

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

Regarding the immune-related studies, we assessed the levels of immunoglobulin and cytokine secretion for 5 consecutive weeks. Interleukin-2 and interferon-γ mediate cellular immunity. IL-2 stimulates the proliferation of activated B cells to secrete immunoglobulin, and at the same time induces the growth and differentiation of T cells to produce IFN-γ. In addition, interleukin-2 enhanced the cytotoxicity of T lymphocytes and natural killer cells. INF-γ activates macrophages to engulf antigens and specifically kill pathogenic microorganisms. IL-4 is mainly produced by activated T-nuclear monocytes, stimulates B-cell proliferation, promotes and regulates IgE production, stimulates T-cell growth, and induces many inflammatory responses.

As can be seen from Tables 4 to 9, the contents of immune factors IL-2, IL-4, IFN-γ, Ig-A, IgG and Ig-M in the experimental animal serum of the experimental group and the control group were between 1 and 4. week showed a gradually increasing trend, and reached the peak in the 4th week, and the contents of the above indicators in the experimental group 1 were higher than that of the control group 1, and the contents of the experimental group 2 were higher than that of the control group 2; better than the control group. Supplementing new nutrients keeps enhancing the immune function, which can significantly increase the level of immunoglobulin in the blood.

In order to obtain the best immune effect, it is necessary to provide adequate and balanced nutrition. Different physiological stages require different elements, resulting from differences in bioavailability, metabolic levels, and degrees of loss. But for the control group 1, from the 1st, 2nd week to the 5th week of feeding, the content of immune indexes began to increase, while in the other groups, after the 1st week of feeding, the level of immune factors increased significantly, indicating that the immunity was significantly improved. high. Comparing the experimental groups 3, 4, and 5, it can be seen that the zinc citrate group, the zinc citrate and zinc gluconate (calculated as zinc) ratio of 1:1 group has a good level of 6 immune indicators, which is better than that of the zinc gluconate group . It shows that the nutrients of simulated human milk are more easily absorbed.

In addition, the contents of the active ingredients in the experimental groups 3-5 are equal, and the experimental group 5 is equivalent to half of the sum of the experimental groups 3 and 4 in terms of active ingredients. As can be seen from Table 5 and 8-9, from the first week to the fifth week, the results of the experimental group 5 are generally better than half of the sum of the results of the experimental group 3 and the experimental group 4, showing that in zinc gluconate and lemon There is a synergistic effect between zinc and acid zinc.

2.2 The content of vitamin A, vitamin E and vitamin _B1 in serum

Table 10 Contents of Vitamin A, Vitamin E, and Vitamin _B1

Note: *p<0.05 compared with the blank group, **p<0.01 compared with the blank group, ***p<0.001 compared with the blank group

As can _be seen from Table 10, the contents of vitamin A, vitamin E and vitamin _B1 in the serum of the experimental group rats are all higher than the corresponding matched group; The rate is better than that of vitamin A, vitamin E and vitamin B ₁ used in the control group.

In addition, it can also be seen from the above table that in terms of the contents of vitamin A, vitamin E, and vitamin _B1 in the serum of rats, from the first week to the fifth week, the results of the experimental group 4 were better than those of the experimental group 3. The results show that the effect of zinc citrate is better than that of zinc gluconate in promoting the absorption of vitamin A, vitamin E, and vitamin _B1 .

In addition, it can also be seen from the above table that the results of experimental group 5 are better than half of the sum of the results of experimental group 3 and experimental group 4, indicating that there is a synergistic effect between zinc gluconate and zinc citrate, which can synergistically Promote the absorption of vitamin A, vitamin E, and vitamin _B1 .

It can be seen from Table 11 that the contents of calcium, zinc and iron in the liver, kidney and bone of the rats in the experimental group are higher than those in the corresponding control group, indicating that the calcium, zinc and iron used in the nutrients in the experimental group are superior in digestion and absorption. used in the control group.

In addition, it can also be seen from the above table that in terms of the contents of calcium, zinc and iron in the liver, kidney and bone of the rats, the results of the experimental group 4 were better than those of the experimental group 3 from the first week to the fifth week The results showed that the effect of zinc citrate was better than that of zinc gluconate.

In addition, it can also be seen from the above table that the results of experimental group 5 are better than half of the sum of the results of experimental group 3 and experimental group 4, indicating that there is a synergistic effect between zinc gluconate and zinc citrate, which can synergistically Promotes the absorption of calcium, zinc, iron in the liver, kidneys and bones.

In summary, retinyl palmitate, d-alpha-tocopheryl acetate, thiamine hydrochloride, ferrous gluconate, gluconic acid Zinc and/or zinc citrate, and the combination of calcium citrate will obviously be better than retinyl acetate, retinyl acetate, Combination of dl-alpha-tocopheryl acetate, thiamine nitrate, ferrous sulfate, zinc sulfate, calcium carbonate.

Claims

A nutritional composition for formula food, which comprises or consists of the following:

(1) Compound vitamins, which include:

Vitamin A, part or all of which is derived from retinyl palmitate, for example all of which is derived from retinyl palmitate;

Vitamin E, partly or wholly derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, e.g. wholly derived from d-alpha-tocopheryl acetate and/or d-alpha-tocopherol, e.g. whole source in d-alpha-tocopheryl acetate; and

Vitamin B1 , partly or wholly derived from thiamine hydrochloride, for example entirely derived from thiamine hydrochloride,

(2) Compound minerals, which include:

Iron, a part or all of which is derived from ferrous gluconate, for example, iron is derived from ferrous gluconate or a combination of ferrous gluconate and one or more of ferrous fumarate, ferric citrate, and ferric pyrophosphate, such as all derived from ferrous gluconate, and

Zinc, partly or wholly derived from zinc gluconate and/or zinc citrate, such as wholly derived from zinc gluconate and/or zinc citrate, such as zinc derived from zinc gluconate, zinc citrate, or zinc citrate and glucose Zinc acid,

and

(3) Calcium citrate.
The nutritional composition of claim 1, wherein:

With respect to 1 mass part of calcium citrate,

The amount of vitamin A based on the active ingredient RE is 0.00008-0.007, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass;

The amount of vitamin E based on the active ingredient is 0.001-0.1, preferably 0.002-0.08, preferably 0.01-0.05 parts by mass; and

The amount of vitamin B1 in terms of active ingredients is 0.0001-0.01, preferably 0.0002-0.008, preferably 0.001-0.005 parts by mass,

and / or

With respect to 1 mass part of calcium citrate,

The amount of iron element is 0.0005-0.15, preferably 0.001-0.10, preferably 0.005-0.02 parts by mass; and

The amount of zinc element is 0.0005-0.10, preferably 0.001-0.05, preferably 0.005-0.02 parts by mass.
The nutritional composition according to any one of claims 1-2, wherein:

The compound vitamin further comprises one or more of the following: vitamin D, vitamin K 1 , vitamin B 2 , vitamin B 6 , vitamin B 12 , niacin, folic acid, pantothenic acid, vitamin C, and biotin,

Preferably, the compound vitamin further comprises vitamin D, vitamin K 1 , vitamin B 2 , vitamin B 6 , vitamin B 12 , niacin, folic acid, pantothenic acid, vitamin C, and biotin;

Preferably, when present,

Some or all of the vitamin D is derived from cholecalciferol, for example all from cholecalciferol, and/or

part or all of the vitamin K1 is derived from phytonadiones, for example all of which are derived from phytonadiones, and/or

Some or all of the vitamin B2 is derived from riboflavin, for example entirely from riboflavin, and/or

A part or all of vitamin B6 is derived from pyridoxine hydrochloride and/or 5'-pyridoxal phosphate, for example all is derived from pyridoxine hydrochloride and/or 5'-pyridoxal phosphate, for example all is derived from pyridoxine hydrochloride; and / or

Some or all of the vitamin B12 is derived from cyanocobalamin, for example entirely from cyanocobalamin; and/or

Some or all of the niacin is derived from niacinamide, for example all of it is derived from niacinamide; and/or

Some or all of the folic acid is derived from folic acid, such as entirely derived from folic acid; and/or

Some or all of the pantothenic acid is derived from calcium D-pantothenate, for example all is derived from D-calcium pantothenate; and/or

Part or all of vitamin C is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate and/or L-ascorbic acid, for example all is derived from L-sodium ascorbate; and/or

Part or all of biotin is derived from D-biotin, such as all is derived from D-biotin.
The nutritional composition of claim 3, wherein, when present, relative to 1 part by mass of calcium citrate, in terms of active ingredient,

The amount of vitamin D is 0.000002-0.0002, preferably 0.000004-0.00015, preferably 0.00002- 0.0001 parts by mass;

The amount of vitamin K1 is 0.00001-0.001, preferably 0.00005-0.0008, preferably 0.0001-0.0005 parts by mass;

The amount of vitamin B2 is 0.00008-0.005, preferably 0.0001-0.003, preferably 0.0005-0.002 parts by mass;

The amount of vitamin B6 is 0.00006-0.007, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass;

The amount of vitamin B12 is 0.0000002-0.00003, preferably 0.000001-0.00001, preferably 0.000002-0.000008 parts by mass;

The amount of niacin is 0.0006-0.05, preferably 0.001-0.04, preferably 0.005-0.02 parts by mass;

The amount of folic acid is 0.00001-0.002, preferably 0.00002-0.001, preferably 0.0001-0.0005 parts by mass;

The amount of pantothenic acid is 0.0005-0.03, preferably 0.001-0.02, preferably 0.005-0.01 parts by mass;

The amount of vitamin C is 0.015-1.2, preferably 0.02-1.0, preferably 0.10-0.50 parts by mass; and

The amount of biotin is 0.000001-0.0002, preferably 0.00001-0.0001, preferably 0.00001-0.00008 parts by mass.
The nutritional composition according to any one of claims 1-4, wherein:

The compound minerals further include one or more of the following elements: magnesium, manganese, iodine, copper, selenium;

Preferably, the compound minerals further include magnesium, manganese, iodine, copper, and selenium;

Preferably, when present,

Some or all of the magnesium is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate and/or magnesium gluconate, for example all is derived from magnesium sulphate; and/or

Some or all of the manganese is derived from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all is derived from manganese sulphate and/or manganese citrate and/or manganese gluconate, e.g. all is derived from magnesium sulphate; and/or or

Some or all of the iodine is derived from potassium iodate, for example entirely from potassium iodate; and/or

Some or all of the copper is derived from copper sulphate and/or copper citrate and/or copper gluconate, for example all is derived from copper sulphate and/or copper citrate and/or copper gluconate, for example all is derived from copper sulphate; and/or or

Part or all of selenium is derived from sodium selenite, for example, all of it is derived from sodium selenite.
The composition of any one of claims 1-5, wherein, when present, relative to 1 part by mass of calcium citrate,

The amount of magnesium element is 0.001-0.2, preferably 0.005-0.1, preferably 0.01-0.05 parts by mass;

The amount of manganese element is 0.000006-0.0006, preferably 0.00001-0.0004, preferably 0.00005-0.0002 parts by mass;

The amount of iodine element is 0.000003-0.0015, preferably 0.00005-0.001, preferably 0.0001-0.0005 parts by mass;

The amount of copper element is 0.00004-0.006, preferably 0.0001-0.005, preferably 0.0005-0.002 parts by mass; and

The amount of selenium element is 0.000001-0.0002, preferably 0.000002-0.0002, preferably 0.00001-0.0001 parts by mass.
The nutritional composition according to any one of claims 1-6, wherein every gram of compound vitamin comprises:

Vitamin A: 550-4600 μg RE, preferably 1080-3080 μg RE,

Vitamin E: 9.0-73.0mg α-TE, preferably 18-49mg α-TE,

Vitamin B1 : 1.0-7.0mg, preferably 1.9-4.8mg,

Vitamin D: 18.0-136.0 μg, preferably 36.6-91.0 μg,

Vitamin K1 : 70-670 μg, preferably 138-448 μg,

Vitamin B2 : 550-3500 μg, preferably 890-2600 μg,

Vitamin B6 : 0.45-5.0mg, preferably 0.8-3.4mg,

Vitamin B12 : 1.8-16.0 μg, preferably 3.6-11.0 μg,

Niacin: 4.5-37.5mg, preferably 8.0-26.0mg,

Folic acid: 90-1100μg, preferably 180-790μg,

Pantothenic acid: 3.5-25.0mg, preferably 6.0-17.6mg,

Vitamin C: 105-900mg, preferably 185-650mg, and

Biotin: 12.0-130.0 μg, preferably 21.0-86.8 μg;

and / or

Each gram of mixed minerals includes the following elements:

Iron: 6.0-89.0mg, preferably 12.0-59.5mg,

Zinc: 3.0-75.0mg, preferably 7.0-55.2mg,

Magnesium: 10.0-120mg, preferably 19.8-79.0mg,

Manganese: 45-400 μg, preferably 84-294 μg,

Iodine: 20-900 μg, preferably 40-560 μg,

Copper: 300-4200 μg, preferably 600-2800 μg, and

Selenium: 10-130 μg, preferably 24-98 μg.
The nutritional composition according to any one of claims 1-7, wherein:

Relative to 1 mass part of calcium citrate, the amount of compound vitamins is 0.15-1.33 mass parts, preferably 0.2-1.1 mass parts; and

The amount of the compound mineral is 0.15-1.33 parts by mass, preferably 0.2-1.1 parts by mass, relative to 1 part by mass of calcium citrate.
A formula food, preferably infant formula food, preferably infant formula milk powder, children's formula modulated milk or milk powder, or adult formula modulated milk or milk powder, comprising the nutritional combination according to any one of claims 1-8 The nutritional composition is preferably added in an amount of 0.6-1.8% by mass, preferably 0.7-1.4% by mass in the formula food.
The nutritional composition as claimed in any one of claims 1-8 or the formula food as claimed in claim 9 can enhance the immunity of the body and promote digestion and absorption, especially for vitamin A, vitamin E and vitamin B1 and iron, zinc and Non-therapeutic use in digestion and absorption of calcium.