WO2023098392A1 - 一种流水全自动免疫检测系统及其检测方法 - Google Patents

一种流水全自动免疫检测系统及其检测方法 Download PDF

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Publication number
WO2023098392A1
WO2023098392A1 PCT/CN2022/129718 CN2022129718W WO2023098392A1 WO 2023098392 A1 WO2023098392 A1 WO 2023098392A1 CN 2022129718 W CN2022129718 W CN 2022129718W WO 2023098392 A1 WO2023098392 A1 WO 2023098392A1
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Prior art keywords
unit
reagent
arm
sample
needle
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PCT/CN2022/129718
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English (en)
French (fr)
Inventor
王国锋
孟雄
洪龙斌
庞建强
张玉
孙辰晛
周旭一
Original Assignee
中翰盛泰生物技术股份有限公司
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Publication of WO2023098392A1 publication Critical patent/WO2023098392A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00594—Quality control, including calibration or testing of components of the analyser
    • G01N35/00613—Quality control
    • G01N35/00663—Quality control of consumables
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/0092—Scheduling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes
    • G01N2035/00752—Type of codes bar codes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0418—Plate elements with several rows of samples
    • G01N2035/0425—Stacks, magazines or elevators for plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0474—Details of actuating means for conveyors or pipettes
    • G01N2035/0491—Position sensing, encoding; closed-loop control
    • G01N2035/0494—Detecting or compensating piositioning errors

Definitions

  • the invention relates to a fully automatic immune detection system, in particular to a running water fully automatic immune detection system and a detection method thereof.
  • This device is an instrument-dependent POCT automatic quantitative system. It realizes the identification of various sample types and the possible pretreatment of samples through a unique sampling unit. Strip stack unit), complete the sample addition and the entire dry fluorescence reaction process, and finally complete the detection. It realizes the automation, informatization, integration, individualization and modular rapid detection of POCT. Various sample types can be directly tested on the machine, which meets the development requirements of hospital informatization and the multi-purpose requirements and quality assurance of clinical emergency. . He has the following characteristics:
  • the temperature is controllable throughout the reaction process
  • the device can perform multi-indicator detection at the same time, and the project covers other cardiovascular diseases, infectious diseases, women's health, infection, embolism and other rapid detection and has obtained demonstration applications.
  • This device is a modular device that can be used alone or online. It is also scalable and can be interconnected with the same type of equipment (meeting the interconnection requirements).
  • This device is a rare immune detection assembly line in the industry. The detection speed of a single unit is above 120T/H, and the speed should exceed that of the same dry-type immune analyzer in the same industry. It ensures that JS3000 can adapt to various sample types and multi-indicator detection , while reflecting the advantages of full-process temperature control, it greatly improves the detection speed and application flexibility, can adapt to the application needs of more customers, reduces the waiting time of customers, and further increases the convenience of use.
  • the object of the present invention is to provide a fully automatic flow immunological detection system and a detection method thereof for the defects of the prior art.
  • a fully automatic immunoassay system for flowing water comprising an area to be tested, a barcode identification area, a transmission area, a plurality of detection areas, a placement area, a controller and a power supply, the detection area is composed of a sampling/recovery unit, a conveyor belt unit, a shaking Uniform unit, lath stack unit, cuvette transfer unit, sample arm, reagent arm, reagent chamber unit, incubation unit, liquid circuit unit, waste recovery unit, detection unit;
  • a number of samples to be tested are placed in the area to be tested, and the samples to be tested are placed in test tubes on the test tube holder.
  • the test tube is provided with an identification barcode corresponding to the information of the current sample to be tested.
  • the sampling area is connected with the sampling/recovery unit of the detection area, the reset area and the detection area, and one side of the sampling/recovery unit is connected with the transfer area, which is used for the transfer of the samples to be tested and the samples that have been tested between the transfer area and the detection area. Transfer, the other side of the sampling/recovery unit is provided with a conveyor belt unit, which is used to transfer the samples to be tested to the shaking unit one by one.
  • the channel of the delivery area is provided with a barcode identification area, and the conveyor belt unit is connected to the shaking unit.
  • a sample arm is provided above the shaking unit, a slat stacking unit is provided on one side of the sample arm, a slat transfer mechanism is provided on the slat stacking unit, and a cuvette is arranged above the slat stacking unit
  • the cuvette transfer unit is provided with a cuvette transfer device, the cuvette transfer device is adjacent to the reagent arm, a waste recovery unit is provided below the cuvette transfer unit;
  • a reagent arm is provided on one side A warehouse unit, the reagent arm is connected to the liquid circuit unit, an incubation unit is provided on the other side of the reagent arm, a detection unit is provided on one side of the incubation unit, the lath stack unit, incubation unit and A push mechanism is provided between the detection units, and the push mechanism is a push rod. Through the movement of the push rod, the slats are a
  • a sensor and a push rod are provided between the transfer area, the reset area, and the sample injection/recovery unit.
  • the sensor is used to detect whether the rack carrying the sample is in place.
  • the push rod is activated to push the rack into the unit.
  • the pulling tabs are retractable movable tabs, and the tabs are equipped with electronic switches. In the process, the guide rails on both sides are fully extended, and after the sample holder is pushed and pulled to the designated position, the pull tabs retract to both sides of the channel.
  • the shaking unit includes a test tube clamp, an anti-off strip, a shaking motor, a Z-axis motor, and a Y-axis motor.
  • An anti-off strip is provided above the test tube clamp to prevent the test tubes in the test tube clamp from falling off during the shaking process.
  • the shaker motor is movably connected with the test tube clamp, and the test tube clamp is shaken with the rotation of the shaker motor, and the Z-axis motor is movably connected with the shaker motor through the belt on the Z-axis motor bracket and the runner.
  • the Y-axis motor is movably connected with the Z-axis motor through the belt and the runner on the Y-axis motor bracket, so that the test tube clamp can move back and forth, up and down, and realize the action of lifting and lowering the test tube from the bracket.
  • the slat stacking unit includes a card frame, a card taking arm, a push rod, a card frame scanner and a card frame scanner bracket, and the slats (cards) are placed in the card frame, and different card frames are placed in different card frames.
  • the slats of different testing items there is a card scanner on the rear side of the card holder, which is used to distinguish the test items and the number of slats in the card holder.
  • the card holder scanner is set on the card holder On the bracket, a card-taking arm bracket is provided below the card frame scanner bracket, a push rod and a card-taking arm are arranged on the card-taking arm bracket, and a motor is provided under the card-taking arm to make the card-taking arm rotate.
  • the card-taking arm is provided with a card seat, and a card-taking telescopic rod is provided under the card seat.
  • One end of the card-taking telescopic rod is provided with a card-taking hook, which is a telescopic movable hook.
  • the telescopic rod for taking out the card extends forward along the bottom guide rail of the card holder, and the hook for taking out the card is pushed against the bottom of the card holder and extends forward. , the hook slides smoothly into the groove, hooks the bottom slat (card) of the card holder, and the height of the hook is consistent with the thickness of the slat (card).
  • the reaction cup transfer unit includes a drawer, a reaction cup holder, a gripper, a reaction cup holder, a movable cup holder, a gripper moving unit, and a reaction cup holder guide rail, and the one and/or multiple reaction cup holders are arranged in the drawer, Empty cuvettes are stored in the cuvette holder, the gripper is arranged above the cuvette holder, the gripper is connected to the gripper moving unit through the gripper bracket, and the cuvette holder is connected to the reaction cup holder through the movable cup holder.
  • the cup holder guide rail is connected, the gripper moving unit includes a gripper bracket and a movable bracket, and the movable bracket is controlled by a motor to move forward and backward, and the gripper bracket is set on the movable bracket and the X-direction motor passes through a belt and a transmission wheel Movably connected to control the left and right movement of the gripper.
  • the gripper bracket is provided with a Z-direction motor.
  • the cup holders are movably connected, and the movable cup holder can move along the guide rail.
  • the reaction cup holder is provided with a reaction cup holder, the reaction cup holder is provided with a return spring, and the gripper is provided with a solenoid valve and a gripper.
  • the reaction cup guide rail is arranged on the guide rail support, and the reagent arm is movably connected to the guide rail support through a motor, a belt and a transmission wheel.
  • the reagent arm is provided with a double-needle support, and the double-needle support is provided with a reagent needle. and a waste liquid needle, the reagent needle and the waste liquid needle are connected with a liquid circuit unit.
  • the sample arm includes a sample needle, a stirring device, a movable rod, a motor bracket, an arm group rotation motor and an arm group lifting motor
  • the sample needle is arranged on a sample needle base
  • the sample needle base is connected with a movable rod
  • the A stirring device is provided on the movable rod
  • the movable rod is connected with the motor bracket of the arm group.
  • the motor bracket of the arm group is provided with an arm group rotating motor and an arm group lifting motor
  • the sample needle seat is provided with an anti-collision device.
  • the anti-collision device is arranged on the top of the sample needle, and a buffer space is artificially set to prevent the hard damage caused by the contact of the needle head when the sample needle moves up and down.
  • the stirring device is provided with a DC motor, and the DC motor is connected with an eccentric
  • a sample needle hole is provided on the eccentric sheet, and a needle washing pool is arranged below the sample needle hole.
  • the sample needle moves up and down through the sample needle through hole.
  • the through hole is set on the eccentric plate.
  • the DC motor drives the eccentric plate, the sample needle around the sample needle through the hole rotates, and at the same time it drives the sample needle to stir; the needle washing pool under the sample needle A cleaning fluid is connected to wash the outer wall of the sample needle.
  • the arm group rotating motor and the arm group lifting motor on the arm group motor bracket are connected with the movable rod through the transmission wheel and the belt.
  • the lifting and rotation of the movable rod is realized through the positive and negative rotation of the motor, and at the same time, the lifting and rotation of the sample needle seat is driven.
  • the reagent warehouse unit includes a heat dissipation fan, a heat sink, a reagent bottle, a warehouse body, a thermal insulation layer, a window, a bar code machine, a sensor, a cooling plate, and an air guiding device.
  • the bottom of the plate is connected with a bearing, and the bearing is connected with a stepping motor and a stepping sensor.
  • the motor is connected with the bearing to drive the entire warehouse body to rotate.
  • the reagent plate is provided with a gap corresponding to the barcode on the reagent bottle.
  • a bar code machine is provided on one side of the window, and when the bin body is rotated, the bar code machine can read the information of each reagent bottle through the rotation of the bin body, and the center of the reagent plate is provided with a heat sink.
  • a heat dissipation fan is arranged above the heat sink, and a refrigerating sheet is arranged below the warehouse body, and the refrigerating sheet is connected with an air guiding device, and a stepping motor and a stepping sensor are connected under the reagent plate, and the motor connecting bearing can drive
  • the entire bin body rotates, and an insulation layer is provided outside the bin body, and the insulation layer is thermal insulation cotton, and the bottom of the bin body is connected with a liquid circuit unit for discharging condensed water generated during refrigeration.
  • the liquid circuit unit includes a reagent needle inner and outer cleaning liquid circuit, a sample needle inner clean water cleaning liquid circuit, a sample needle outer lotion cleaning liquid circuit, and a waste liquid discharge liquid circuit;
  • the internal and external cleaning liquid path of the reagent needle includes a clean water bucket, a first pump, a first valve and a first needle washing pool connected in sequence, the reagent needle is arranged in the first needle washing pool, and the first valve is connected with the first needle washing pool all the way.
  • the plunger pump is connected to the inside of the reagent needle through the reagent needle interface for cleaning the inner wall of the reagent needle, and the first valve is connected to the first needle washing pool for cleaning the outer wall of the reagent needle.
  • the first plunger pump can perform Extraction and discharge of reagent solutions;
  • the clean water cleaning liquid path inside the sample needle includes a clean water bucket, a second pump, a second valve, a second plunger pump and a fourth valve connected in sequence, the second plunger pump is connected to the inside of the sample needle through the sample needle interface, and the fourth plunger pump is connected to the inside of the sample needle.
  • the valve When the valve is selected, the clean water inside the sample needle cleans the inner wall of the reagent needle, and the second plunger pump can extract and discharge the sample solution through the sample needle;
  • the washing liquid cleaning liquid circuit outside the sample needle includes a washing liquid barrel, a third pump, and a third valve connected in sequence.
  • the sample needle is arranged in the second needle washing pool, and the third valve is connected to the outer wall of the second needle washing pool. When the three valves are selected, the cleaning fluid path outside the sample needle cleans the outer wall of the sample needle.
  • the liquid circuit system also includes a condensate and waste liquid discharge circuit.
  • the condensate and waste liquid discharge liquid circuit includes a waste liquid needle connected in sequence, a fifth pump and a fifth valve, and the fifth valve passes through the reagent all the way.
  • the disk interface is connected to the reagent disk, and the other is connected to the waste liquid needle through the waste liquid needle interface.
  • the fifth pump is used to discharge the condensate in the reagent disk and the waste liquid sucked by the waste liquid needle into the waste liquid bucket.
  • the bottom of the first needle washing pool is connected to the fourth pump, and the fourth pump is used to discharge the waste liquid from the first needle washing pool to the waste liquid barrel.
  • the bottom of the second needle washing pool is connected to the sixth pump, and the sixth pump is used to discharge the waste liquid from the second needle washing pool to the waste liquid bucket.
  • Filters and connection joints are also provided between the clean water bucket and the first pump and the second pump.
  • a filter and a connecting joint are also provided between the washing liquid bucket and the third pump.
  • a filter is provided between the reagent disc and the fifth valve.
  • the incubation unit includes an incubation rack, a thermostat, and an incubation sensor.
  • the incubation rack is provided with a plurality of spaces for placing slats (cards), and the thermostat is used to heat problems in the incubation rack.
  • the sensor detects the ambient temperature in the incubation frame at all times, and the incubation unit controls the movement of the incubation frame through the incubation frame motor and the incubation frame guide rail to correspond to the horizontal position of the slats (cards) pushed by the push rod.
  • the detection unit includes a detection deck, a detection deck bracket, a detector arranged above the detection deck, the detection deck bracket moves along the guide rail through a motor, a belt and a transmission wheel, and the push rod meets the temperature of the incubation unit.
  • the slats (cards) of the incubation time are pushed into the detection card holder, and the detection card holder bracket drives the detection card holder to move under the detector for detection.
  • the push rod pushes the slats (cards) to the waste hole, and the waste hole and The waste recovery unit is connected.
  • a flowing water automatic immunoassay method comprising a test area, a barcode identification area, a transmission area, a plurality of detection areas, a placement area, a controller and a power supply
  • the detection area is composed of a sampling/recovery unit, a conveyor belt unit, a shaker
  • the system composed of uniform unit, strip stacking unit, cuvette transfer unit, sample arm, reagent arm, reagent chamber unit, incubation unit, liquid circuit unit, waste recycling unit and detection unit is realized through the following steps:
  • Step 1 Transfer the samples in the area to be tested through the transfer area. During the transfer, scan the code to obtain the current sample information to be tested.
  • the test tube where the sample is located is placed on the sample holder, and the corresponding barcode is pasted on the test tube arm;
  • Step 2 obtains an empty cuvette from the cuvette transfer unit
  • Step 3 Transfer the current sample to be tested to the sample injection/recovery unit in the corresponding detection area according to the obtained sample information to be tested, the slat stacking unit prepares the corresponding slats, and the reagent compartment unit prepares the corresponding reagents;
  • Step 4 The sample to be tested enters the detection area, and the shaking unit grabs the samples to be tested on the bracket in turn and shakes them well, and puts them back into the brackets in sequence after shaking well;
  • Step 5 The reagent arm draws the prepared reagent from the reagent chamber unit through the reagent needle and injects it into the cuvette. After completion, the reagent needle on the reagent arm passes through the liquid circuit unit to clean the inside and body of the needle;
  • Step 6 The sample arm draws the shaken sample to be tested through the sample needle, pours it into the cuvette and stirs it. After all the samples to be tested on the bracket are completed, the sample to be tested is sent to the storage area through the sample injection/recovery unit;
  • Step 7 The sample arm draws the stirred mixed solution through the sample needle and adds it to the prepared slat. After completion, the sample needle passes through the liquid circuit unit to clean the inside and the needle body, and the reagent arm uses the waste liquid needle to extract the residual solution in the cuvette. Solution, and then push the empty cuvette to the waste recycling unit through the waste liquid needle itself. After completion, the waste liquid needle cleans the waste liquid needle itself through the liquid circuit unit;
  • step 8 the strips to which the mixed solution is added are sent to the incubation unit and the incubation time is set simultaneously;
  • Step 9 Send the slats that meet the incubation time into the detection unit for detection, and discard the slats through the waste hole on the detection unit after completion;
  • Step 10 obtains the final detection information.
  • the beneficial effects of the present invention are: adapting to various sample types, multi-indicators and multi-threads, pipeline asynchronous execution detection, greatly improving detection speed and application flexibility, and being able to adapt to more customer applications requirements, while reducing the detection waiting time, further increasing the convenience of use.
  • Fig. 1 is a schematic diagram of the structure of the detection area in a flowing water automatic immunoassay system provided by the present invention
  • Fig. 2 is a schematic structural diagram of the shaking unit provided by the present invention.
  • Fig. 3 is a schematic structural diagram of a cuvette transfer unit provided by the present invention.
  • Fig. 4 is a schematic structural diagram of a sample arm provided by the present invention.
  • Fig. 5 is a schematic structural diagram of the reagent arm and part of the cuvette transfer unit provided by the present invention.
  • Fig. 6 is a schematic diagram of the structure of the reagent chamber unit provided by the present invention.
  • Fig. 7 is a schematic diagram of the liquid circuit structure in the detection area provided by the present invention.
  • Fig. 8 is a structural schematic diagram of a slat stacking unit provided by the present invention.
  • Fig. 9 is a schematic diagram of the structure of the incubation unit provided by the present invention.
  • Fig. 10 is a schematic structural diagram of a detection unit provided by the present invention.
  • Fig. 11 is a schematic structural diagram of a flow-through automatic immunoassay system provided by the present invention.
  • test tube clamp 201
  • anti-off strip 203
  • shaking motor 204
  • Z-axis motor 205
  • Y-axis motor
  • reaction cup holder, 302 gripper, 303, reaction cup holder, 304, spring, 305, X direction motor, 306, Z direction motor, 307, movable cup holder, 308, reaction cup holder guide rail, 309, drawer, 310, gripper support, 311, movable support,
  • cooling fan 602, heat sink, 603, reagent bottle, 604, reagent plate, 605, bearing, 606, warehouse body, 607, thermal insulation cotton, 608, window, 609, bar code machine, 610, sensor, 611, refrigeration Sheet, 612, air guiding device,
  • card holder 801, card holder, 802, card taking arm, 803, card taking hook, 804, card taking telescopic rod, 805, card seat, 806, card taking arm bracket, 807, push rod, 808, card holder code scanner, 809 , Card Holder Scanner Bracket,
  • a kind of flowing water automatic immunoassay detection method comprises to-be-tested area 15, barcode identification area 16, transmission area 17, a plurality of detection areas 13, placement area 14, controller and power supply, described detection area 13 is controlled by sampling/recovery Unit 11, Conveyor Belt Unit 12, Shaking Unit 2, Plate Stacking Unit 8, Reaction Cup Transfer Unit 3, Sample Arm 4, Reagent Arm 5, Reagent Chamber Unit 6, Incubation Unit 9, Liquid Circuit Unit 7, Waste Recovery Unit , detection unit 10 in the system formed by the following steps to achieve:
  • Step 1 The sample in the area to be tested is transmitted through the transmission area 17, and the current sample information to be tested is obtained through the barcode identification area 16 during transmission.
  • the test tube where the sample is located is placed on the sample holder, and the corresponding test tube arm is pasted. barcode identification;
  • Step 2 obtains an empty cuvette from the cuvette transfer unit 3;
  • Step 3 transmits the current sample to be tested to the sample injection/recovery unit 11 corresponding to the detection area 13 according to the obtained sample information to be tested. Due to the wide variety of detection items, blank slats corresponding to different detections are stored in different detection areas, and at the same time Each detection area is implemented to detect the remaining number of slats, and when the remaining items detect that the blank slats are insufficient, the system automatically assigns the current sample to be tested to the detection area 13 with the corresponding blank slats, and the slat stacking unit 8 prepares the corresponding slats. The reagent compartment unit 6 prepares corresponding reagents;
  • Step 4 The sample to be tested enters the designated detection area 13, and the shaking unit 2 sequentially grabs the sample to be tested on the bracket and shakes it well, and puts it back into the bracket in sequence after shaking well;
  • Step 5 The reagent arm 5 extracts the prepared reagent from the reagent chamber unit 6 through the reagent needle and injects it into the cuvette, and after completion, the reagent needle on the reagent arm 5 passes through the liquid circuit unit 7 to clean the inside of the needle and the needle body;
  • Step 6 The sample arm 4 extracts the shaken sample to be tested through the sample needle and pours it into the cuvette and stirs it. After all the samples to be tested on the bracket are completed, the sample to be tested is sent to the storage area 14 through the sample injection/recovery unit 11;
  • Step 7 The sample arm 4 extracts the stirred mixed solution through the sample needle and adds it to the prepared slat. After completion, the sample needle passes through the liquid circuit unit 7 to clean the inside of the needle and the needle body, and the reagent arm 5 extracts the reaction through the waste liquid needle. The remaining solution in the cup, and then push the empty reaction cup to the waste recycling unit through the waste liquid needle itself. After completion, the waste liquid needle cleans the waste liquid needle itself through the liquid circuit unit 7;
  • Step 8 sends the slat to which the mixed solution is added into the incubation unit 9 and sets the incubation time at the same time;
  • Step 9 Send the slats meeting the incubation time into the detection unit 10 for detection, and discard the slats through the waste hole 1004 on the detection unit after completion;
  • Step 10 obtains the final detection information.
  • a kind of running water automatic immunoassay system comprising a region to be tested 15, a barcode identification region 16, a transfer region 17, a plurality of detection regions 13, a placement region 14, a controller and a power supply, and the detection region 13 is controlled by sample injection/recovery Unit 11, Conveyor Belt Unit 12, Shaking Unit 2, Plate Stacking Unit 8, Reaction Cup Transfer Unit 3, Sample Arm 4, Reagent Arm 5, Reagent Chamber Unit 6, Incubation Unit 9, Liquid Circuit Unit 7, Waste Recovery Unit , detection unit 10 is formed;
  • a number of samples to be tested are placed in the area to be tested 15, and the samples to be tested are placed in the test tubes on the test tube support, and the test tubes are provided with identification barcodes corresponding to the current sample information to be tested.
  • the sample injection/recovery unit 11 of the detection area 13, the placement area 15 and the detection area 13 is connected through the transfer area 17, and one side of the sample introduction/recovery unit 11 is connected with the transfer area 17 for the transfer area 17 and the detection area 13 to transfer the sample to be tested and the sample to be tested, the other side of the sampling/recovery unit 11 is provided with a conveyor belt unit 12, which is used to transfer the samples to be tested to the shaking unit 2 one by one, on the channel of the transfer area 17
  • a barcode identification area 16 is provided, the conveyor belt unit 12 is adjacent to the shaking unit 2, a sample arm 4 is arranged above the shaking unit 2, and a slat stacking unit 8 is arranged on one side of the sample arm 4.
  • the slat stacking unit 8 is provided with a slat transfer mechanism, and a cuvette transfer unit 3 is arranged above the slat stack unit 8, and the cuvette transfer unit 3 is provided with a cuvette transfer device, and the cuvette transfer device and the reagent
  • the arms 5 are adjacent, and a waste recovery unit is arranged below the cuvette transfer unit 3; a reagent chamber unit 6 is arranged on one side of the reagent arm 5, and the reagent arm 5 is connected with a liquid circuit unit 7, and the reagent arm 5, an incubation unit 9 is provided on the other side, and a detection unit 10 is provided on one side of the incubation unit 9, and a pushing mechanism is provided between the slat stacking unit 8, the incubation unit 9 and the detection unit 10, and the The pushing mechanism is a push rod, and the strip is pushed from the strip stacking unit 8 to the incubation unit 9 and the detection unit 10 through the movement of the push rod, and the sample arm 4 and the reagent arm 5 are
  • a sensor and a push rod are arranged between the transfer area 17, the reset area 14, and the sample injection/recovery unit 11.
  • the sensor is used to detect whether the support that currently carries the sample is in place. After it is in place, the push rod is activated to push the support into the sample injection unit.
  • the area to be tested 15, the reset area 14, and both sides of the inner channel of the sampling/recovery unit 11 are respectively provided with pull tabs.
  • the pull tabs are retractable movable pull tabs.
  • the pull tabs are provided with electronic switches. During the stroke movement of the push-pull sample holder, it is fully extended along the guide rails on both sides. After the sample holder is pushed and pulled to the designated position, the pull tab retracts to both sides of the channel.
  • the shaking unit 2 includes a test tube clamp 201, an anti-off strip 202, a shaking motor 203, a Z-axis motor 204, and a Y-axis motor 205.
  • An anti-off strip 202 is provided above the test tube clamp 201 to prevent the test tube clamp from shaking during the shaking process.
  • the test tube in 201 falls off, and the shaker motor 203 is flexibly connected with the test tube holder 201, and the test tube holder 201 shakes evenly with the rotation of the shaker motor 203, and the Z-axis motor 204 passes through the belt on the Z-axis motor support.
  • test tube holder 201 It is movably connected with the runner and the shaking motor 203, and the Y-axis motor 205 is movably connected with the runner and the Z-axis motor 204 through the belt on the Y-axis motor bracket, so that the test tube holder 201 can move back and forth, up and down, so that the test tube can be moved from The action of lifting and lowering on the bracket.
  • the slat stacking unit 8 includes a card frame 801, a card-taking arm 802, a push rod 807, a card frame scanner 808 and a card frame scanner bracket 809, and a slat (card) is placed in the card frame 801, and different There are slats corresponding to different testing items placed in the card frame 801, and a card frame scanner 808 is provided on the rear side of the card frame 801, which is used to distinguish the items and the number of slats tested by the slats in the card frame.
  • the device 808 is arranged on the card frame scanner bracket 809, the card frame scanner bracket 809 is provided with a card removal arm bracket 806, and the card removal arm bracket 806 is provided with a push rod 807 and a card removal arm 802,
  • a motor is provided under the card-taking arm 802 to rotate the card-taking arm 802.
  • a card holder 805 is provided on the card-taking arm 802.
  • a card-taking telescopic rod 804 is arranged below the card-taking arm 805.
  • the card-taking telescopic rod 804 One end of 804 is provided with a grab hook 803, and the grab hook 803 is a telescopic movable hook.
  • the telescopic rod 804 for taking a card stretches forward along the guide rail at the bottom of the card holder, and the grab hook 803 is pushed against the card holder As the bottom extends forward, the bottom of the card holder 801 is provided with a card removal groove. When the removal hook 803 reaches the card removal notch at the bottom of the card holder 801, the hook slides smoothly into the groove, and the hook at the bottom of the card holder 801 is hooked. Slats (cards), the height of the hooks is consistent with the thickness of the slats (cards).
  • the reaction cup transfer unit 3 includes a drawer 309, a reaction cup holder 301, a handle 302, a reaction cup holder 303, a movable cup holder 307, a gripper moving unit, and a reaction cup holder guide rail 308.
  • the one and/or more reaction cup holders 301 is set in the drawer 309, the cuvette rack 301 stores empty cuvettes, the gripper 302 is arranged above the cuvette rack 301, and the gripper 302 is connected to the gripper mobile unit through the gripper bracket 310 , the cuvette holder 303 is connected to the cuvette holder guide rail 308 through the movable cup holder 307, the gripper moving unit includes a gripper bracket 310 and a movable bracket 311, and the movable bracket 311 moves back and forth through the motor control movable bracket 311,
  • the grip bracket 310 is arranged on the movable bracket 311 and is movably connected with the X-direction motor 305 through a belt and the transmission wheel to control the left and right movement of the gripper 302.
  • the Z-direction motor 306 is arranged on the gripper bracket 310, and the Z direction
  • the motor 306 is movably connected with the gripper 302 through a belt and a drive wheel to control the up and down movement of the gripper 302.
  • the guide rail is movably connected with the movable cup holder 307, and the movable cup holder 307 can move along the guide rail.
  • the movable cup holder 307 There is a cuvette seat 303 on it, the cuvette seat 303 is provided with a return spring 304, the handle 302 is provided with a solenoid valve and grippers, the cuvette guide rail 308 is arranged on a guide rail bracket, and on the guide rail bracket
  • the reagent arm 5 is movably connected to the reagent arm 5 through a motor, a belt, and a transmission wheel.
  • a double-needle support 508 is provided on the reagent arm 5.
  • a reagent needle 501 and a waste liquid needle 502 are provided on the double-needle support 508. The reagent The needle 501 and the waste liquid needle 502 are connected with the liquid circuit unit 7 .
  • the sample arm 4 includes a sample needle 401, a stirring device 403, a movable rod 410, an arm group motor support 409, an arm group rotating motor 407 and an arm group lifting motor 408, the sample needle 401 is arranged on a sample needle seat 411, and the sample Needle seat 411 is connected with movable rod 410, and described movable rod 410 is provided with stirring device 403, and described movable rod 410 is connected with arm group motor support 409, and described arm group motor support 409 is provided with arm group rotating motor 407 and
  • the arm group lifting motor 408, the sample needle seat 411 is provided with an anti-collision device 402, and the anti-collision device 402 is arranged on the top of the sample needle 401, and a buffer space is artificially set to prevent the sample needle 401 from being touched by the needle when it is moving up and down.
  • the stirring device 403 is provided with a DC motor 404, the DC motor 404 is connected with an eccentric plate, the eccentric plate is provided with a sample needle through hole, and a needle washing needle is provided below the sample needle through hole pool.
  • the sample needle 401 moves up and down through the sample needle through hole.
  • the through hole is set on the eccentric plate.
  • the arm group rotating motor 407 and the arm group lifting motor 408 on the arm group motor bracket are connected with the movable rod through the transmission wheel and the belt.
  • the lifting and rotation of the movable rod is realized through the positive and negative rotation of the motor, and at the same time, the lifting and rotation of the sample needle seat is driven.
  • a code disc 406 is provided between the movable rod 410 and the arm group rotation motor 407, and the code disc 406 is used for calibration. Sample pin rocker arm rotation in degrees.
  • Reagent warehouse unit 6 comprises radiating fan 601, cooling fin 602, reagent bottle 603,606 storehouse body, insulation layer, window 608, bar code machine 609, sensor 610, cooling sheet 611 and air guiding device 612, described multiple reagent bottle 603
  • the fan-shaped distribution is arranged in the reagent tray 604, the bottom of the reagent tray 604 is connected with a bearing, the bearing is connected with a stepping motor and a stepping sensor, and the motor is connected to the bearing to drive the entire warehouse body 606 to rotate.
  • the reagent tray 604 is provided with a The gap corresponding to the bar code on the reagent bottle 603, the warehouse body 606 is provided with a window corresponding to the gap, and a bar code machine 609 is provided on one side of the window, and the warehouse body 606 is rotated, and the bar code machine 609 can pass through the rotation of the warehouse body 606.
  • the center of the reagent plate 604 is provided with a cooling fin 602
  • the top of the cooling fin 602 is provided with a cooling fan 601
  • the bottom of the warehouse body 606 is provided with a cooling sheet 611
  • the The refrigerating plate 611 is connected with an air guiding device 612
  • a stepping motor and a stepping sensor are connected under the reagent plate 604, and the motor is connected to a bearing to drive the entire bin body 606 to rotate
  • the bin body 606 is provided with an insulating layer outside
  • the The insulation layer is insulation cotton 607
  • the bottom of the bin body 606 is connected to the liquid circuit unit 7 for discharging condensed water generated during refrigeration.
  • the liquid path unit 7 includes a cleaning liquid path inside and outside the reagent needle, a clear water cleaning liquid path inside the sample needle, a washing liquid cleaning liquid path outside the sample needle, and a waste liquid discharge liquid path;
  • the internal and external cleaning fluid circuit of the reagent needle 501 includes a clear water bucket 720, a first pump 701, a first valve 702 and a first needle washing pool connected in sequence, the reagent needle 501 is arranged in the first needle washing pool, and the first needle washing pool
  • One way of the valve 702 is connected to the first plunger pump 703 through the reagent needle interface 709 to clean the inner wall of the reagent needle 502, and the other way of the first valve 702 is connected to the first needle washing pool to clean the outer wall of the reagent needle 501.
  • the first plunger pump 703 can extract and discharge the reagent solution through the reagent needle 501;
  • the clean water cleaning liquid path inside the sample needle 401 includes a clean water bucket 720, a second pump 704, a second valve 705, a second plunger pump 706, and a fourth valve 711 connected in sequence, and the second plunger pump 706 passes through the sample needle interface. 715 is connected to the inside of the sample needle 401.
  • the fourth valve 711 is selected, the clean water cleaning liquid path inside the sample needle 401 cleans the inner wall of the sample needle 401, and the second plunger pump 706 can extract and discharge the sample solution through the sample needle;
  • the washing liquid cleaning liquid circuit outside the sample needle 401 includes a washing liquid barrel 721, a third pump 707 and a third valve 708 connected in sequence.
  • the sample needle 401 is set in the second needle washing pool, and the third valve 708 is connected to the second The outer wall of the needle washing pool, when the third valve 708 is selected, the outer wall of the sample needle 401 is cleaned by the washing liquid cleaning liquid path outside the sample needle 401 .
  • the liquid circuit system also includes a condensate and waste liquid discharge circuit.
  • the condensate and waste liquid discharge circuit includes a waste liquid needle 502 connected in sequence, a fifth pump 712 and a fifth valve 713.
  • the fifth valve 713 one way is connected to the reagent plate through the reagent plate interface 716, and the other way is connected to the waste liquid needle 502 through the waste liquid needle interface 717, and the fifth pump 712 is used to discharge the condensate in the reagent plate and the waste liquid absorbed by the waste liquid needle 502 to the waste bucket 722.
  • the bottom of the first needle washing pool is connected to the fourth pump 710 , and the fourth pump 710 is used to discharge the waste liquid from the first needle washing pool to the waste liquid bucket 722 .
  • the bottom of the second needle washing pool is connected to the sixth pump 715 , and the sixth pump 715 is used to discharge the waste liquid from the second needle washing pool to the waste liquid barrel 722 .
  • a filter 719 and a connecting joint 718 are also provided between the clean water bucket 720 and the first pump 701 and the second pump 704 .
  • a filter and a connection joint are also provided between the washing liquid bucket 721 and the third pump 707 .
  • a filter is provided between the reagent disc and the fifth valve 713 .
  • the incubation unit includes an incubation rack 901, a thermostat 902, and an incubation sensor 903.
  • the incubation rack 901 is provided with a plurality of spaces for placing slats (cards), and the thermostat 902 is used to heat the incubation rack.
  • the incubation sensor 903 detects the ambient temperature in the incubation frame 901 at all times, and the incubation unit 9 controls the movement of the incubation frame 901 through the incubation frame motor 904 and the incubation frame guide rail 905 to correspond to the push rod 807.
  • the detection unit 10 comprises a detection deck 1001, a detection deck support 1002, a detector 1003 arranged above the detection deck 1001, the detection deck support 1002 moves along the guide rail through a motor, a belt and a drive wheel, and the push rod 807 will Incubation unit 9 pushes the slats (cards) that meet the incubation time into the detection deck 1001, and the detection deck bracket 1002 drives the detection deck 1001 to move under the detector 1003 for detection. After the detection, the push rod 807 pushes the slats (card) is pushed to the waste material hole 1004, and the waste material hole 1004 is connected with the waste material recovery unit.
  • a kind of running water automatic immunoassay system and its detection method of the present invention can be used for the determination of various detection items, such as: C-reactive protein determination, neutrophil gelatinase-associated lipocalin determination, ⁇ -human chorion Gonadotropin assay, D-dimer assay, procalcitonin assay, heparin-binding protein assay, whole-course C-reactive protein assay, troponin I assay, creatine kinase isoenzyme assay, amino-terminal brain natriuretic peptide precursor Determination, progesterone determination, serum amyloid A determination, heart-type fatty acid binding protein determination, troponin I determination, B-type natriuretic peptide determination, myoglobin determination, soluble growth-stimulating expression gene 2 protein determination, neutrophil Determination of cell gelatinase-associated lipocalin, lipoprotein-associated phospholipase A2, urine microalbumin, anti-Müllerian hormone

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Abstract

一种流水全自动免疫检测系统及其检测方法，系统包括待测区（15）、条码识别区（16）、传送区（17）、多个检测区（13）、归置区（14）、控制器和电源，检测区（13）由进样/回收单元（11）、传送带单元（12）、摇匀单元（2）、板条堆栈单元（8）、反应杯转移单元（3）、样本臂（4）、试剂臂（5）、试剂仓单元（6）、温育单元（9）、液路单元（7）、废料回收单元、检测单元（10）组成；该流水全自动免疫检测系统及其检测方法，可适应多种样本类型、多指标多线程，流水式异步执行检测，极大的提高了检测速度和应用灵活性，能够适应更多的客户的应用需求，同时减少了检测等待时间，进一步的增加了使用便利性。

Description

一种流水全自动免疫检测系统及其检测方法 技术领域
本发明涉及全自动免疫检测系统，尤其涉及一种流水全自动免疫检测系统及其检测方法。
背景技术
本装置是依赖于仪器的POCT全自动定量系统，通过独特的进样单元实现了多种样本类型的识别以及样本可能需要的前处理，同时通过加样单元和耗材单元(反应杯转移单元和反应板条堆栈单元)，完成样本的添加以及整个干式荧光反应的过程，最终完成检测。它实现了POCT的自动化、信息化、集成化、个性化和模块化的快速检测，多种样本类型可直接上机进行检测，满足医院信息化的发展要求和临床急诊的多用途需求和质量保证。他具有以下特点：
可以支持多种样本直接上机检测，原始管直接上机检测，无需开盖，规避了生物安全风险，并且无需繁琐的人工样本上机前处理；
整个反应过程，温度可控；
本装置可以进行多指标同时检测，项目覆盖别心血管疾病、感染性疾病、妇女健康、传染、栓塞等快速检测上获得示范应用。
本装置是模块化设备，可以单独使用，也可以联机使用，同时具有可扩展性，可以和同类型设备(满足互联要求的)互联使用。本装置是行业内少有的免疫检测流水线，检测速度单台120T/H以上，速率应同样的干式免疫分析仪超过同行的，在保证了JS3000的适应多种样本类型、多指标上机检测，反应全程控温的优点的同时，极大的提高了检测速度和应用灵活性，能够适应更多的客户的应用需求，同时减少了客户的等待时间，进一步的增加了使用便利性。
发明内容
本发明的目的是针对现有技术的缺陷，提供了全自动流水免疫检测系统及其检测方法。
为了实现以上目的，本发明采用以下技术方案：
一种流水全自动免疫检测系统，包括待测区、条码识别区、传送区、多个检测区、归置区、控制器和电源，所述检测区由进样/回收单元、传送带单元、摇匀单元、板条堆栈单元、反应杯转移单元、样本臂、试剂臂、试剂仓单元、温育单元、液路单元、废料回收单元、检测单元组成；
所述待测区内放置有若干待测样本，所述待测样本置于试管支架上的试管内，所述试管上设有当前待测样本信息对应的识别条码，所述待测区通过传送区与检测区、归置区和检测区的进样/回收单元连接，所述进样/回收单元一侧与传送区相连，用于传送区与检测区之间待测样本和已测样本的传递，进样/回收单元的另一侧设有传送带单元，用于逐一传递待测样本给摇匀单元，所述传送区的通道上设有条码识别区，所述传送带单元与摇匀单元相邻，所述摇匀单元上方设有样本臂，所述样本臂一侧设有板条堆栈单元，所述板条堆栈单元设有板条传送机构，所述板条堆栈单元上方设有反应杯转移单元，所述反应杯转移单元设有反应杯传送装置，所述反应杯传送装置与试剂臂相邻，所述反应杯转移单元下方设有废料回收单元；所述试剂臂一侧设有试剂仓单元，所述试剂臂上与液路单元连接，所述试剂臂另一侧设有温育单元，所述温育单元一侧设有检测单元，所述板条堆栈单元、温育单元和检测单元之间设有推送机构，所述推送机构为推送杆，通过推送杆的移动，将板条从板条堆栈单元推送至温育单元和检测单元，所述样本臂和试剂臂与液路单元相连。
进一步，传送区与归置区、进样/回收单元之间设有传感器和推杆，传感器用于检测当前承载样本的支架是否到位，到位后启动推杆，推送支架进入单元，所述待测区、归置区和进样/回收单元内通道两侧分别设有拉片，所述拉片为可伸缩的活动拉片，拉片设有电子开关，开启后拉片推拉样本支架的行程移动中，沿着两侧内的导轨完全展开，将样本支架 推拉到指定位置后，拉片缩回通道两侧。
进一步，摇匀单元包括试管夹、防脱条、摇匀电机、Z轴电机、Y轴电机，所述试管夹上方设有防脱条，防止在摇匀过程中试管夹内的试管脱落，所述摇匀电机与试管夹活动连接，试管夹随着摇匀电机的转动进行摇匀的活动，所述Z轴电机通过Z轴电机支架上的皮带与转轮与摇匀电机活动连接，所述Y轴电机通过Y轴电机支架上的皮带与转轮与Z轴电机活动连接，使试管夹能前后，上下移动，实现将试管从支架上提起，放下的动作。
进一步，板条堆栈单元包括卡架、取卡臂、推杆、卡架扫码器和卡架扫码器支架，所述卡架内放置有板条(卡)，不同的卡架内放置有对应不同检测项目的板条，卡架后侧设有卡架扫码器，用于区分卡架内板条测试的项目及板条数量，所述卡架扫码器设置在卡架扫码器支架上，所述卡架扫码器支架下方设有取卡臂支架，所述取卡臂支架上设有推杆和取卡臂，取卡臂下设有电机，可使取卡臂转动，所述取卡臂上设有卡座，所述卡座下方设有取卡伸缩杆，所述取卡伸缩杆一端设有取卡钩，取卡钩为伸缩活动卡钩，取板条时，取卡伸缩杆沿着卡架底部导轨向前伸展，取卡钩抵住卡架底部随着向前延伸，卡架底部设有取卡槽，当取卡钩抵达卡架底部的取卡槽口时，卡钩顺利滑入槽内，钩取卡架最底部的板条(卡)，卡钩高度与板条(卡)厚度相符。
进一步，反应杯转移单元包括抽屉、反应杯架、抓手、反应杯座、活动杯架、抓手移动单元、反应杯座导轨，所述一个和/或多个反应杯架设置于抽屉内，反应杯架内存放有空的反应杯，所述反应杯架上方设有所述抓手，所述抓手通过抓手支架与抓手移动单元连接，所述反应杯座通过活动杯架与反应杯座导轨连接，所述抓手移动单元包括抓手支架和活动支架，所述活动支架通过电机控制活动支架前后移动，所述抓手支架设置在活动支架上与X向电机通过皮带与传动轮活动连接，控制抓手的左右移动，所述抓手支架上设置有Z向电机，所述Z向电机与抓手通过皮带和传动轮活动连接，控制抓手的上下移动，所述导轨与活动杯架活动相连，活动杯架可沿着导轨移动，所述活动杯架上设有反应杯座，所述反 应杯座设有复位弹簧，所述抓手设有电磁阀和抓钳，所述反应杯导轨设置在导轨支架上，所述导轨支架上通过电机、皮带和传动轮活动连接有所述试剂臂，所述试剂臂上设有双针支架，所述双针支架上设有试剂针和废液针，所述试剂针和废液针连接有液路单元。
进一步，样本臂包括样本针、搅拌装置、活动杆、电机支架、臂组旋转电机和臂组升降电机，所述样本针设置在样本针座上，所述样本针座连接有活动杆，所述活动杆上设有搅拌装置，所述活动杆与臂组电机支架连接，所述臂组电机支架上设有臂组旋转电机和臂组升降电机，所述样本针座上设有防撞装置，所述防撞装置设与样本针的顶部，人为设置缓冲空间，防止样本针在升降活动时，针头触抵，产生的硬损伤，所述搅拌装置上设直流电机，所述直流电机连接有偏心片，所述偏心片上设有样本针过孔，所述样本针过孔下方设有洗针池。样本针通过样本针过孔进行升降活动，过孔设于偏心片上，当直流电机带动偏心片，使得环绕于样本针的样本针过孔转动，同时带动样本针搅动；样本针下方的洗针池连接有清洗液，能对样本针外壁进行冲洗，所述臂组电机支架上的臂组旋转电机和臂组升降电机通过传动轮与皮带与活动杆连接。通过电机的正反转来实现活动杆的升降与旋转，同时带动样本针座的升降与旋转，所述活动杆与臂组旋转电机之间设有码盘，通过码盘来校准样本针摇臂旋转度数。
进一步，试剂仓单元包括散热风扇、散热片、试剂瓶、仓体、保温层、视窗、条码机、传感器、制冷片和导风装置，所述试剂瓶扇形分布设置在试剂盘内，所述试剂盘底部连接有轴承，轴承连接有步进电机和步进传感器，电机连接轴承可带动整个仓体转动，所述试剂盘上设有与试剂瓶上条码对应的缺口，所述仓体上设有与该缺口对应的视窗，所述视窗一侧设有条码机，转动仓体，条码机可以通过仓体的转动，读取到每一个试剂瓶的信息，所述试剂盘中心设有散热片，所述散热片上方设有散热风扇，所述仓体下方设有制冷片，所述制冷片连接有导风装置，所述试剂盘下方连接有步进电机和步进传感器，电机连接轴承可带动整个仓体转动，所述仓体外设有保温层，所述保温层为保温棉，仓体底部与液路 单元连接，用于排放制冷时所产生的冷凝水。
进一步，所述液路单元包括试剂针内外清洗液路、样本针内侧清水清洗液路、样本针外侧洗液清洗液路和废液排放液路；
所述试剂针内外清洗液路包括依次连通的清水桶、第一泵、第一阀及第一洗针池，所述试剂针设于第一洗针池内，所述第一阀一路与第一柱塞泵通过试剂针接口连接试剂针内部用于清洗试剂针的内壁，第一阀另一路连接第一洗针池用于清洗试剂针的外壁，所述第一柱塞泵通过试剂针可进行试剂溶液的抽取和排放；
所述样本针内侧清水清洗液路包括依次连通的清水桶、第二泵、第二阀、第二柱塞泵及第四阀，第二柱塞泵通过样本针接口连接样本针内部，第四阀选通时，样本针内侧清水清洗液路清洗试剂针的内壁，所述第二柱塞泵通过样本针可进行样本溶液的抽取和排放；
所述样本针外侧洗液清洗液路包括依次连通的洗液桶、第三泵及第三阀，所述样本针设于第二洗针池内，第三阀连接第二洗针池外壁，第三阀选通时，样本针外侧洗液清洗液路清洗样本针的外壁。
所述液路系统还包括冷凝液、废液排放液路，所述冷凝液、废液排放液路包括依次连通的废液针，第五泵及第五阀，所述第五阀一路通过试剂盘接口连接试剂盘，另一路通过废液针接口连接废液针，所述第五泵用于将试剂盘内的冷凝液及废液针吸取的废液排放至废液桶中。
所述第一洗针池底部连接第四泵，第四泵用于将第一洗针池的废液排放至废液桶。
所述第二洗针池底部连接第六泵，第六泵用于将第二洗针池的废液排放至废液桶。
所述清水桶与第一泵和第二泵之间还设有过滤器及连接接头。
所述洗液桶与第三泵之间还设有过滤器及连接接头。
所述试剂盘与第五阀之间设有过滤器。
进一步，温育单元包括温育架，恒温器、温育传感器，所述温育架设置有多个安放板 条(卡)的空间，所述恒温器用于加热温育架内的问题，温育传感器时刻检测温育架内的环境温度，温育单元通过温育架电机和温育架导轨控制温育架的移动，来对应推杆所推送过来的板条(卡)的水平位置。
进一步，检测单元包括检测卡座、检测卡座支架，设置在检测卡座上方的检测器，所述检测卡座支架通过电机、皮带和传动轮沿着导轨移动，推杆将温育单元满足温育时间的板条(卡)推送至检测卡座内，检测卡座支架带动检测卡座移动到检测器下进行检测，检测完毕后推杆将板条(卡)推送至废料孔，废料孔与废料回收单元相连。
一种流水全自动免疫检测方法，包括待测区、条码识别区、传送区、多个检测区、归置区、控制器和电源，所述检测区由进样/回收单元、传送带单元、摇匀单元、板条堆栈单元、反应杯转移单元、样本臂、试剂臂、试剂仓单元、温育单元、液路单元、废料回收单元、检测单元组成的系统中通过以下步骤实现：
步骤1.将待测区内的样本通过传送区进行传送，在传送时通过扫码得到当前待测样本信息，样本所在的试管置于样本支架上，试管臂上黏贴有对应的条码标识；
步骤2从反应杯转移单元获取空的反应杯；
步骤3根据得到的待测样本信息将当前待测样本传送到对应检测区的进样/回收单元，板条堆栈单元准备对应的板条，试剂仓单元准备对应的试剂；
步骤4待测样本进入检测区，摇匀单元依次抓取支架上的待测样本摇匀，摇匀后依次放回支架；
步骤5试剂臂通过试剂针从试剂仓单元内抽取准备好的试剂注入反应杯，完成后对试剂臂上的试剂针通过液路单元对针内和针体进行清洗；
步骤6样本臂通过样本针抽取摇匀后的待测样本注入反应杯并搅拌，支架上的待测样本都完成后，通过进样/回收单元将已测样本送至归置区；
步骤7样本臂通过样本针抽取搅拌后的混合溶液添加到准备好的板条上，完成后样本 针通过液路单元对针内和针体进行清洗，试剂臂通过废液针抽取反应杯内残余溶液，然后通过废液针自身将空反应杯推送至废料回收单元，完成后，废液针通过液路单元对废液针自身进行清洗；
步骤8将添加混合溶液的板条送入温育单元同时设置温育时间；
步骤9将满足温育时间的板条送入检测单元进行检测，完成后通过检测单元上的废料孔丢弃板条；
步骤10获得最终检测信息。
采用本发明技术方案，本发明的有益效果为：适应多种样本类型、多指标多线程，流水式异步执行检测，极大的提高了检测速度和应用灵活性，能够适应更多的客户的应用需求，同时减少了检测等待时间，进一步的增加了使用便利性。
附图说明
图1是本发明提供的一种流水全自动免疫检测系统中检测区结构示意图；
图2是本发明提供的摇匀单元结构示意图；
图3是本发明提供的反应杯转移单元结构示意图；
图4是本发明提供的样本臂结构示意图；
图5是本发明提供的试剂臂和部分反应杯转移单元结构示意图；
图6是本发明提供的试剂仓单元结构示意图；
图7是本发明提供的检测区液路结构示意图；
图8是本发明提供的板条堆栈单元结构示意图；
图9是本发明提供的温育单元结构示意图；
图10是本发明提供的检测单元结构示意图；
图11是本发明提供的一种流水全自动免疫检测系统结构示意图。
其中，1、设备支架，2、摇匀单元，3、反应杯转移单元，4、样本臂，5、试剂臂，6、 试剂仓单元，7、液路单元，8、板条堆栈单元，9、温育单元，10、检测单元，11、进样/回收单元，12、传送带单元，13、检测区，14、归置区，15、待测区，16、条码识别区，17、传送区
201、试管夹，202、防脱条，203、摇匀电机，204、Z轴电机，205、Y轴电机，
301、反应杯架，302、抓手，303、反应杯座，304、弹簧，305、X向电机，306、Z向电机，307、活动杯架，308、反应杯座导轨，309、抽屉，310、抓手支架，311、活动支架，
401、样本针，402、防撞装置，403、搅拌装置，404、直流电机，405、洗针池，406、码盘，407、臂组旋转电机，408、臂组升降电机，409、臂组电机支架，410、活动杆，411、样本针座，
501、试剂针，502、废液针，503、双针电机，504、双针电机支架，505、导轨电机，506、试剂针泵，507、废液针泵，508、双针支架，509、导轨支架，
601、散热风扇，602、散热片，603、试剂瓶，604、试剂盘，605、轴承，606、仓体，607、保温棉，608、视窗，609、条码机，610、传感器，611、制冷片，612、导风装置，
701、第一泵，702、第一阀，703、第一柱塞泵，704、第二泵，705、第二阀，706、第二柱塞泵，707、第三泵，708、第三阀，709、试剂针接口，710、第四泵，711、第四阀，712、第五泵，713、第五阀，714、第六泵，715、样本针接口，716、试剂盘接口，717、废液针接口，718、接头，719、过滤器，720、清水桶，721、洗液桶，722、废液桶，
801、卡架，802、取卡臂，803、取卡钩，804、取卡伸缩杆，805、卡座，806、取卡臂支架，807、推杆，808、卡架扫码器，809、卡架扫码器支架，
901、温育架，902、恒温器，903、温育传感器，904、温育架电机，905温育架导轨，
1001、检测卡座，1002、检测卡座支架，1003、检测器，1004、废料孔。
具体实施方式
下面结合附图和具体实施方式对本发明作进一步详细说明。
一种流水全自动免疫检测方法，包括待测区15、条码识别区16、传送区17、多个检测区13、归置区14、控制器和电源，所述检测区13由进样/回收单元11、传送带单元12、摇匀单元2、板条堆栈单元8、反应杯转移单元3、样本臂4、试剂臂5、试剂仓单元6、温育单元9、液路单元7、废料回收单元、检测单元10组成的系统中通过以下步骤实现：
步骤1.将待测区内的样本通过传送区17进行传送，在传送时通过条码识别区16得到当前待测样本信息，样本所在的试管置于样本支架上，试管臂上黏贴有对应的条码标识；
步骤2从反应杯转移单元3获取空的反应杯；
步骤3根据得到的待测样本信息将当前待测样本传送到对应检测区13的进样/回收单元11，由于检测项目种类繁多，不同的检测区内存放有对应不同检测的空白板条，同时各检测区实施检测板条所剩数量，所剩项目检测空白板条不足时，系统自动分配当前待测样本去有对应空白板条的检测区13，板条堆栈单元8准备对应的板条，试剂仓单元6准备对应的试剂；
步骤4待测样本进入指定检测区13，摇匀单元2依次抓取支架上的待测样本摇匀，摇匀后依次放回支架；
步骤5试剂臂5通过试剂针从试剂仓单元6内抽取准备好的试剂注入反应杯，完成后对试剂臂5上的试剂针通过液路单元7对针内和针体进行清洗；
步骤6样本臂4通过样本针抽取摇匀后的待测样本注入反应杯并搅拌，支架上的待测样本都完成后，通过进样/回收单元11将已测样本送至归置区14；
步骤7样本臂4通过样本针抽取搅拌后的混合溶液添加到准备好的板条上，完成后样本针通过液路单元7对针内和针体进行清洗，试剂臂5通过废液针抽取反应杯内残余溶液，然后通过废液针自身将空反应杯推送至废料回收单元，完成后，废液针通过液路单元7对废液针自身进行清洗；
步骤8将添加混合溶液的板条送入温育单元9同时设置温育时间；
步骤9将满足温育时间的板条送入检测单元10进行检测，完成后通过检测单元上的废料孔1004丢弃板条；
步骤10获得最终检测信息。
一种流水全自动免疫检测系统，包括待测区15、条码识别区16、传送区17、多个检测区13、归置区14、控制器和电源，所述检测区13由进样/回收单元11、传送带单元12、摇匀单元2、板条堆栈单元8、反应杯转移单元3、样本臂4、试剂臂5、试剂仓单元6、温育单元9、液路单元7、废料回收单元、检测单元10组成；
所述待测区15内放置有若干待测样本，所述待测样本置于试管支架上的试管内，所述试管上设有当前待测样本信息对应的识别条码，所述待测区15通过传送区17与检测区13、归置区15和检测区13的进样/回收单元11连接，所述进样/回收单元11一侧与传送区17相连，用于传送区17与检测区13之间待测样本和已测样本的传递，进样/回收单元11的另一侧设有传送带单元12，用于逐一传递待测样本给摇匀单元2，所述传送区17的通道上设有条码识别区16，所述传送带单元12与摇匀单元2相邻，所述摇匀单元2上方设有样本臂4，所述样本臂4一侧设有板条堆栈单元8，所述板条堆栈单元8设有板条传送机构，所述板条堆栈单元8上方设有反应杯转移单元3，所述反应杯转移单元3设有反应杯传送装置，所述反应杯传送装置与试剂臂5相邻，所述反应杯转移单元3下方设有废料回收单元；所述试剂臂5一侧设有试剂仓单元6，所述试剂臂5上与液路单元7连接，所述试剂臂5另一侧设有温育单元9，所述温育单元9一侧设有检测单元10，所述板条堆栈单元8、温育单元9和检测单元10之间设有推送机构，所述推送机构为推送杆，通过推送杆的移动，将板条从板条堆栈单元8推送至温育单元9和检测单元10，所述样本臂4和试剂臂5与液路单元7相连。
传送区17与归置区14、进样/回收单元11之间设有传感器和推杆，传感器用于检测当 前承载样本的支架是否到位，到位后启动推杆，推送支架进入进样单元，所述待测区15、归置区14和进样/回收单元11内通道两侧分别设有拉片，所述拉片为可伸缩的活动拉片，拉片设有电子开关，开启后拉片推拉样本支架的行程移动中，沿着两侧内的导轨完全展开，将样本支架推拉到指定位置后，拉片缩回通道两侧。
摇匀单元2包括试管夹201、防脱条202、摇匀电机203、Z轴电机204、Y轴电机205，所述试管夹201上方设有防脱条202，防止在摇匀过程中试管夹201内的试管脱落，所述摇匀电机203与试管夹201活动连接，试管夹201随着摇匀电机203的转动进行摇匀的活动，所述Z轴电机204通过Z轴电机支架上的皮带与转轮与摇匀电机203活动连接，所述Y轴电机205通过Y轴电机支架上的皮带与转轮与Z轴电机204活动连接，使试管夹201能前后，上下移动，实现将试管从支架上提起，放下的动作。
板条堆栈单元8包括卡架801、取卡臂802、推杆807、卡架扫码器808和卡架扫码器支架809，所述卡架801内放置有板条(卡)，不同的卡架801内放置有对应不同检测项目的板条，卡架801后侧设有卡架扫码器808，用于区分卡架内板条测试的项目及板条数量，所述卡架扫码器808设置在卡架扫码器支架809上，所述卡架扫码器支架809下方设有取卡臂支架806，所述取卡臂支架806上设有推杆807和取卡臂802，取卡臂802下设有电机，可使取卡臂802转动，所述取卡臂802上设有卡座805，所述卡座805下方设有取卡伸缩杆804，所述取卡伸缩杆804一端设有取卡钩803，取卡钩803为伸缩活动卡钩，取板条(卡)时，取卡伸缩杆804沿着卡架底部导轨向前伸展，取卡钩803抵住卡架底部随着向前延伸，卡架801底部设有取卡槽，当取卡钩803抵达卡架801底部的取卡槽口时，卡钩顺利滑入槽内，钩取卡架801最底部的板条(卡)，卡钩高度与板条(卡)厚度相符。
反应杯转移单元3包括抽屉309、反应杯架301、抓手302、反应杯座303、活动杯架307、抓手移动单元、反应杯座导轨308，所述一个和/或多个反应杯架301设置于抽屉309内，反应杯架301内存放有空的反应杯，所述反应杯架301上方设有所述抓手302，所述 抓手302通过抓手支架310与抓手移动单元连接，所述反应杯座303通过活动杯架307与反应杯座导轨308连接，所述抓手移动单元包括抓手支架310和活动支架311，所述活动支架311通过电机控制活动支架311前后移动，所述抓手支架310设置在活动支架311上与X向电机305通过皮带与传动轮活动连接，控制抓手302的左右移动，所述抓手支架310上设置有Z向电机306，所述Z向电机306与抓手302通过皮带和传动轮活动连接，控制抓手302的上下移动，所述导轨与活动杯架307活动相连，活动杯架307可沿着导轨移动，所述活动杯架307上设有反应杯座303，所述反应杯座303设有复位弹簧304，所述抓手302设有电磁阀和抓钳，所述反应杯导轨308设置在导轨支架上，所述导轨支架上通过电机、皮带和传动轮活动连接有所述试剂臂5，所述试剂臂5上设有双针支架508，所述双针支架508上设有试剂针501和废液针502，所述试剂针501和废液针502连接有液路单元7。
样本臂4包括样本针401、搅拌装置403、活动杆410、臂组电机支架409、臂组旋转电机407和臂组升降电机408，所述样本针401设置在样本针座411上，所述样本针座411连接有活动杆410，所述活动杆410上设有搅拌装置403，所述活动杆410与臂组电机支架409连接，所述臂组电机支架409上设有臂组旋转电机407和臂组升降电机408，所述样本针座411上设有防撞装置402，所述防撞装置402设与样本针401的顶部，人为设置缓冲空间，防止样本针401在升降活动时，针头触抵，产生的硬损伤，所述搅拌装置403上设直流电机404，所述直流电机404连接有偏心片，所述偏心片上设有样本针过孔，所述样本针过孔下方设有洗针池。样本针401通过样本针过孔进行升降活动，过孔设于偏心片上，当直流电机404带动偏心片，使得环绕于样本针401的样本针过孔转动，同时带动样本针401搅动；样本针401下方的洗针池连接有清洗液，能对样本针外壁进行冲洗，所述臂组电机支架上的臂组旋转电机407和臂组升降电机408通过传动轮与皮带与活动杆连接。通过电机的正反转来实现活动杆的升降与旋转，同时带动样本针座的升降与旋转，所述活动杆410与臂组旋转电机407之间设有码盘406，通过码盘406来校准样本针摇臂旋转度数。
试剂仓单元6包括散热风扇601、散热片602、试剂瓶603、606仓体、保温层、视窗608、条码机609、传感器610、制冷片611和导风装置612，所述多个试剂瓶603扇形分布设置在试剂盘604内，所述试剂盘604底部连接有轴承，轴承连接有步进电机和步进传感器，电机连接轴承可带动整个仓体606转动，所述试剂盘604上设有与试剂瓶603上条码对应的缺口，所述仓体606上设有与该缺口对应的视窗，所述视窗一侧设有条码机609，转动仓体606，条码机609可以通过仓体606的转动，读取到每一个试剂瓶603的信息，所述试剂盘604中心设有散热片602，所述散热片602上方设有散热风扇601，所述仓体606下方设有制冷片611，所述制冷片611连接有导风装置612，所述试剂盘604下方连接有步进电机和步进传感器，电机连接轴承可带动整个仓体606转动，所述仓体606外设有保温层，所述保温层为保温棉607，仓体606底部与液路单元7连接，用于排放制冷时所产生的冷凝水。
所述液路单元7包括试剂针内外清洗液路、样本针内侧清水清洗液路、样本针外侧洗液清洗液路和废液排放液路；
所述试剂针501内外清洗液路包括依次连通的清水桶720、第一泵701、第一阀702及第一洗针池，所述试剂针501设于第一洗针池内，所述第一阀702一路与第一柱塞泵703通过试剂针接口709连接试剂针502内部用于清洗试剂针的内壁，第一阀702另一路连接第一洗针池用于清洗试剂针501的外壁，所述第一柱塞泵703通过试剂针501可进行试剂溶液的抽取和排放；
所述样本针401内侧清水清洗液路包括依次连通的清水桶720、第二泵704、第二阀705、第二柱塞泵706及第四阀711，第二柱塞泵706通过样本针接口715连接样本针401内部，第四阀711选通时，样本针401内侧清水清洗液路清洗样本针401的内壁，所述第二柱塞泵706通过样本针可进行样本溶液的抽取和排放；
所述样本针401外侧洗液清洗液路包括依次连通的洗液桶721、第三泵707及第三阀 708，所述样本针401设于第二洗针池内，第三阀708连接第二洗针池外壁，第三阀708选通时，样本针401外侧洗液清洗液路清洗样本针401的外壁。
所述液路系统还包括冷凝液、废液排放液路，所述冷凝液、废液排放液路包括依次连通的废液针502，第五泵712及第五阀713，所述第五阀713一路通过试剂盘接口716连接试剂盘，另一路通过废液针接口717连接废液针502，所述第五泵712用于将试剂盘内的冷凝液及废液针502吸取的废液排放至废液桶722中。
所述第一洗针池底部连接第四泵710，第四泵710用于将第一洗针池的废液排放至废液桶722。
所述第二洗针池底部连接第六泵715，第六泵715用于将第二洗针池的废液排放至废液桶722。
所述清水桶720与第一泵701和第二泵704之间还设有过滤器719及连接接头718。
所述洗液桶721与第三泵707之间还设有过滤器及连接接头。
所述试剂盘与第五阀713之间设有过滤器。
温育单元包9括温育架901，恒温器902、温育传感器903，所述温育架901设置有多个安放板条(卡)的空间，所述恒温器902用于加热温育架901内的问题，温育传感器903时刻检测温育架901内的环境温度，温育单元9通过温育架电机904和温育架导轨905控制温育架901的移动，来对应推杆807所推送过来的板条(卡)的水平位置。
检测单元10包括检测卡座1001、检测卡座支架1002，设置在检测卡座1001上方的检测器1003，所述检测卡座支架1002通过电机、皮带和传动轮沿着导轨移动，推杆807将温育单元9满足温育时间的板条(卡)推送至检测卡座1001内，检测卡座支架1002带动检测卡座1001移动到检测器1003下进行检测，检测完毕后推杆807将板条(卡)推送至废料孔1004，废料孔1004与废料回收单元相连。
采用本发明的一种流水全自动免疫检测系统及其检测方法可进行多种检测项目的测 定，如：C反应蛋白测定、中性粒细胞明胶酶相关脂质运载蛋白测定、β-人绒毛膜促性腺激素测定、D-二聚体测定、降钙素原测定、肝素结合蛋白测定、全程C反应蛋白测定、肌钙蛋白I测定、肌酸激酶同工酶测定、氨基末端脑钠肽前体测定、孕酮测定、血清淀粉样蛋白A测定、心型脂肪酸结合蛋白测定、肌钙蛋白I测定、B型利钠肽测定、肌红蛋白测定、可溶性生长刺激表达基因2蛋白测定、中性粒细胞明胶酶相关脂质运载蛋白测定、脂蛋白相关磷脂酶A2测定、尿微量白蛋白测定、抗缪勒管激素测定、β2微球测定、白介素6测定、胃蛋白酶1/2测定等，根据具体项目对摇匀震荡速度和温育反应时间等参数相应调整，皆可通过本发明所述技术来实现。
注意，上述仅为本发明的较佳实施例及所运用技术原理。本领域技术人员会理解，本发明不限于这里所述的特定实施例，对本领域技术人员来说能够进行各种明显的变化、重新调整和替代而不会脱离本发明的保护范围。因此，虽然通过以上实施例对本发明进行了较为详细的说明，但是本发明不仅仅限于以上实施例，在不脱离本发明构思的情况下，还可以包括更多其他等效实施例，而本发明的范围由所附的权利要求范围决定。

Claims (9)

  1. 一种流水全自动免疫检测系统，其特征在于，包括待测区、条码识别区、传送区、多个检测区、归置区、控制器和电源，所述检测区由进样/回收单元、传送带单元、摇匀单元、板条堆栈单元、反应杯转移单元、样本臂、试剂臂、试剂仓单元、温育单元、液路单元、废料回收单元、检测单元组成；
    所述待测区通过传送区与检测区、归置区连接，所述传送区上设有条码识别区，所述检测区设有进样/回收单元，所述进样/回收单元一侧与传送区相连，另一侧设有传送带单元，所述传送带单元与摇匀单元相邻，所述摇匀单元上方设有样本臂，所述样本臂一侧设有板条堆栈单元，所述板条堆栈单元上方设有反应杯转移单元，所述反应杯转移单元与试剂臂相邻，所述反应杯转移单元下方设有废料回收单元，所述试剂臂一侧设有试剂仓单元，所述试剂臂另一侧设有温育单元，所述温育单元一侧设有检测单元，所述板条堆栈单元、温育单元和检测单元之间设有推送机构，所述样本臂和试剂臂与液路单元相连。
  2. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述传送区与归置区、进样/回收单元之间设有传感器和推杆，所述待测区、归置区和进样/回收单元内通道两侧设有拉片，所述拉片为可伸缩的活动拉片。
  3. 如权利要求1所述的一种全自动免疫检测流水系统，其特征在于，所述摇匀单元包括试管夹、防脱条、摇匀电机、Z轴电机、Y轴电机，所述试管夹上方设有防脱条，所述摇匀电机与试管夹活动连接，所述Z轴电机通过Z轴电机支架与摇匀电机活动连接，所述Y轴电机通过Y轴电机支架与Z轴电机活动连接。
  4. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述板条堆栈单元包括卡架、取卡臂、推杆、卡架扫码器和卡架扫码器支架，所述卡架内放置有板条，卡架后侧设有卡架扫码器，所述卡架扫码器设置在卡架扫码器支架上，所述卡架扫码器支架下方设有取卡臂支架，所述取卡臂支架上设有推杆和取卡臂，所述取卡臂上设有卡座，所述卡座下方设有取卡伸缩杆，所述取卡伸缩杆一端设有取卡钩。
  5. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述反应杯转移单元包括抽屉、反应杯架、抓手、反应杯座、活动杯架、抓手移动单元、反应杯座导轨，所述一个和/或多个反应杯架设置于抽屉内，所述反应杯架上方设有所述抓手，所述抓手通过抓手支架与抓手移动单元连接，所述反应杯座通过活动杯架与反应杯座导轨连接，所述抓手移动单元包括抓手支架和活动支架，所述抓手支架设置在活动支架上与X向电机活动连接，所述抓手支架上设置有Z向电机，所述Z向电机与抓手活动连接，所述导轨与活动杯架活动相连，所述活动杯架上设有反应杯座，所述反应杯座设有复位弹簧，所述抓手设有电磁阀和抓钳，所述反应杯导轨设置在导轨支架上，所述导轨支架上活动连接有所述试剂臂，所述试剂臂上设有双针支架，所述双针支架上设有试剂针和废液针，所述试剂针和废液针连接有液路单元。
  6. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述样本臂包括样本针、搅拌装置、活动杆、电机支架、臂组旋转电机和臂组升降电机，所述样本针设置在样本针座上，所述样本针座连接有活动杆，所述活动杆上设有搅拌装置，所述活动杆与电机支架连接，所述电机支架上设有臂组旋转电机和臂组升降电机，所述样本针座上设有防撞装置，所述搅拌装置上设直流电机，所述直流电机连接有偏心片，所述偏心片上设有样本针过孔，所述样本针过孔下方设有洗针池，所述电机支架上的臂组旋转电机和臂组升降电机通过传动轮与皮带与活动杆连接，所述活动杆与臂组旋转电机之间设有码盘，所述样本臂连接有液路单元。
  7. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述试剂仓单元包括散热风扇、散热片、试剂瓶、仓体、保温层、视窗、条码机、传感器、制冷片和导风装置，所述试剂瓶扇形分布设置在剂盘内，所述试剂盘底部连接有轴承，所述试剂盘上设有与试剂瓶上条码对应的缺口，所述仓体上设有与该缺口对应的视窗，所述视窗一侧设有条码机，所述试剂盘中心设有散热片，所述散热片上方设有散热风扇，所述仓体下方设有 制冷片，所述制冷片连接有导风装置，所述试剂盘下方连接有步进电机和步进传感器，所述仓体外设有保温层。
  8. 如权利要求1所述的一种流水全自动免疫检测系统，其特征在于，所述液路单元包括试剂针内外清洗液路、样本针内侧清水清洗液路、样本针外侧洗液清洗液路和废液排放液路。
  9. 一种流水全自动免疫检测方法，其特征在于，包括待测区、条码识别区、传送区、多个检测区、归置区、控制器和电源，所述检测区由进样/回收单元、传送带单元、摇匀单元、板条堆栈单元、反应杯转移单元、样本臂、试剂臂、试剂仓单元、温育单元、液路单元、废料回收单元、检测单元组成的系统中通过以下步骤实现：
    步骤1.将待测区内的样本通过传送区传送，在传送时通过扫码得到当前待测样本信息；
    步骤2从反应杯转移单元获取空的反应杯；
    步骤3根据得到的待测样本信息将当前待测样本传送到对应检测区，板条堆栈单元准备对应的板条，试剂仓单元准备对应的试剂；
    步骤4待测样本进入检测区，摇匀单元将待测样本摇匀；
    步骤5试剂臂从试剂仓单元内抽取准备好的试剂注入反应杯，完成后对试剂臂自身进行清洗；
    步骤6样本臂抽取摇匀后的待测样本注入反应杯并搅拌，完成后，已测样本送至归置区。
    步骤7样本臂抽取搅拌后的混合溶液添加到准备好的板条上，完成后样本臂对自身进行清洗，试剂臂抽取反应杯内残余溶液，将空反应杯推送至废料回收单元，并对自身进行清洗；
    步骤8将添加混合溶液的板条送入温育单元同时设置温育时间；
    步骤9将满足温育时间的板条送入检测单元进行检测，完成后丢弃板条；
    步骤10获得检测信息。
PCT/CN2022/129718 2021-12-03 2022-11-04 一种流水全自动免疫检测系统及其检测方法 WO2023098392A1 (zh)

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