WO2023065299A1 - 显微制样装置及细胞表型控制装置 - Google Patents

显微制样装置及细胞表型控制装置 Download PDF

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Publication number
WO2023065299A1
WO2023065299A1 PCT/CN2021/125686 CN2021125686W WO2023065299A1 WO 2023065299 A1 WO2023065299 A1 WO 2023065299A1 CN 2021125686 W CN2021125686 W CN 2021125686W WO 2023065299 A1 WO2023065299 A1 WO 2023065299A1
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Prior art keywords
plate
sample
forming
preparation device
holes
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Ceased
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PCT/CN2021/125686
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English (en)
French (fr)
Inventor
王雷
张荣荣
郦野
金帆
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Priority to PCT/CN2021/125686 priority Critical patent/WO2023065299A1/zh
Publication of WO2023065299A1 publication Critical patent/WO2023065299A1/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/22—Petri dishes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C12M1/32—Inoculator or sampler multiple field or continuous type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters

Definitions

  • the invention relates to the technical field of micro-experiments, in particular to a micro-sample preparation device and a cell phenotype control device.
  • the following sample preparation process is often used: drop the bacteria/cell suspension on the cut solid medium (such as agar block); etc. After the agar surface is slightly dry, press the side of the agar block dripping the suspension onto a glass coverslip; then place it on an inverted microscope to observe bacteria or cells.
  • the process described above may be referred to as the tableting process.
  • the above experimental process requires manual operations such as cutting the agar block, dropping the bacteria/cell suspension on the agar block, pressing the agar block dripped with the suspension on the glass cover slip, etc.
  • the experimenter must operate carefully to get better results. For example, the glass coverslip may be crushed.
  • the whole experimental process is time-consuming, inefficient, and consumes a lot of time and energy of the experimenter. Operation errors occur, seriously affecting the progress of scientific research or medical diagnosis.
  • an embodiment of the present invention provides a micro sample preparation device and a cell phenotype control device.
  • a micro-sample preparation device comprising: a forming plate having a plurality of forming holes passing through two opposite surfaces of the forming plate for forming a plurality of solid culture media; a well plate having a plurality of sample wells extending across two opposing surfaces of said sample well plate for containing a plurality of said solid media; a cover slip for placement on one surface of said sample well plate; a press plate , a plurality of pressing columns are provided for pressing the plurality of solid culture media in the plurality of shaped holes into the plurality of sample wells and onto the cover glass.
  • a cell phenotype control device including a cell culture plate and the micro-sample preparation device of the foregoing embodiments.
  • the surface of the solid medium is guaranteed to be smooth, and a medium with a higher surface quality is provided for the drop suspension;
  • the liquid medium can be accommodated, and the forming plate can be well supported when the solid medium is prepared, and the forming plate, the first glass sheet and the second glass sheet can also be supported. Play the role of positioning and alignment;
  • the embodiment of the present invention basically dries the solid medium before dropping the suspension by setting a drying rack, shortens the drying time in the process of dropping the suspension, prevents a large amount of water from being produced in the subsequent tableting process, and then avoids the suspension of the suspension.
  • the microorganisms in the medium swim with the water and fail to meet the experimental requirements;
  • the solid culture medium in the sample pool plate is moisturized by setting a moisturizing member to prevent the solid culture gene from being dehydrated and deformed and separated from the cover glass, which is convenient for the experimenter to perform microscopic observation for a long time;
  • the forming plate and the cell culture plate are detachably connected by setting a connecting plate to form a set of cell phenotype control devices that are easy to disassemble and assemble, and are convenient for optogenetics experiments.
  • FIG. 1 is a schematic diagram of an example of some components of a microsample preparation device according to an embodiment of the present invention
  • FIG. 2 is a schematic diagram of an example of a forming plate of a microsample preparation device according to an embodiment of the present invention
  • FIG. 3 is a schematic diagram of an example of a sample pool plate of a microsample preparation device according to an embodiment of the present invention
  • FIG. 4 is a schematic diagram of an example of a choke ring of a microsample preparation device according to an embodiment of the present invention.
  • Fig. 5 is a schematic diagram of an example of a storage box of a microsample preparation device according to an embodiment of the present invention.
  • FIG. 6 is a schematic diagram of an example of a sample preparation component of a micro sample preparation device according to an embodiment of the present invention.
  • FIGS. 7 to 10 are schematic diagrams of the cooperation of components of the microsample preparation device in the sample preparation process according to the embodiment of the present invention.
  • Figure 11 is a cross-sectional view of the assembly in Figure 10.
  • Fig. 12 and Fig. 13 are the schematic diagrams of an example of the drying rack of the microsample preparation device of the embodiment of the present invention.
  • FIGS. 14 to 16 are schematic diagrams of an example of a sample cell plate assembly of a microsample preparation device according to an embodiment of the present invention.
  • Fig. 17 is a schematic diagram of placing the viscous pad between the sample pool plate and the cover glass of the microsample preparation device according to the embodiment of the present invention.
  • Fig. 18 is a schematic diagram of an example of a positioning tray of a microsample preparation device according to an embodiment of the present invention.
  • Fig. 19 and Fig. 20 are schematic diagrams of placing the sample cell plate assembly on the positioning tray of the microsample preparation device according to the embodiment of the present invention.
  • Fig. 21 and Fig. 22 are schematic diagrams of placing the sample cell plate assembly and the forming plate on the positioning tray of the micro sample preparation device according to the embodiment of the present invention.
  • Fig. 23 is a schematic diagram of an example of a platen of a microsample preparation device according to an embodiment of the present invention.
  • Fig. 24 and Fig. 25 are schematic diagrams of the cooperation of the components of the microsample preparation device in the embodiment of the present invention during the tableting process;
  • Fig. 26 and Fig. 27 are schematic diagrams of the cooperation of components of the microsample preparation device of the embodiment of the present invention after tableting;
  • Fig. 28 is a schematic diagram of an example of a moisture-retaining member of a microsample preparation device according to an embodiment of the present invention.
  • Fig. 29 and Fig. 30 are schematic diagrams of cooperation between the moisture-retaining element and the sample pool plate of the microsample preparation device according to the embodiment of the present invention.
  • Fig. 31 is a schematic diagram of an example of the connecting plate of the micro-sample preparation device according to the embodiment of the present invention.
  • Fig. 32 and Fig. 33 are schematic diagrams of an example of a cell phenotype control device according to an embodiment of the present invention.
  • the terms “first”, “second”, etc. are used to distinguish different elements from the name, but do not indicate the spatial arrangement or time order of these elements, and these elements should not be referred to by these Terminology limited.
  • the term “and/or” includes any and all combinations of one or more of the associated listed items.
  • the terms “comprising”, “including”, “having” and the like refer to the presence of stated features, elements, elements or components, but do not exclude the presence or addition of one or more other features, elements, elements or components.
  • Embodiments of the first aspect of the present invention provide a microscopic sample preparation device.
  • the microscopic sample preparation device of the embodiment of the present invention includes a sample preparation assembly and a tablet pressing assembly.
  • a plurality of forming holes 102 are used for forming a plurality of solid medium 200 (as shown in Figure 11), realizes a single preparation of multiple solid medium 200, such as solid medium 200 is an agar block;
  • the pressing assembly includes a sample pool plate 103, a cover glass 104 and a pressing plate 105.
  • the sample pool plate 103 has a plurality of sample holes 106 that run through two opposite surfaces of the sample pool plate 103.
  • the plurality of sample holes 106 can Corresponding to a plurality of forming holes 102 one by one, the cover glass 104 is used to be placed on a surface of the sample cell plate 103, and the pressing plate 105 is provided with a plurality of pressing columns 107, and the plurality of pressing columns 107 can be matched with the plurality of forming holes 102 one by one.
  • a handle 108, handle 108 and pressing column are also provided on the pressing plate 105 107 are respectively arranged on opposite sides of the pressing plate 105 so as to hold the handle 108 to press down the pressing plate 105 .
  • Step S100 Prepare solid medium (such as agar block): as shown in Figure 11, pour the liquid medium (such as agar liquid) into a plurality of forming holes 102 of the forming plate 101, and the liquid medium becomes solid culture after cooling and solidifying Base 200;
  • solid medium such as agar block
  • Step S200 Dropping: drop the bacteria/cell suspension on the surfaces of multiple solid culture media 200 (called the front side);
  • Step S300 sheet pressing: as shown in Figure 24 to Figure 27, place the cover glass 104 on the bottom surface of the sample pool plate 103, place the forming plate 101 on the top surface of the sample pool plate 103 (the front side of the solid medium 200 faces Next), the pressing plate 105 is placed on the forming plate 101, at this time, the pressing column 107, the forming hole 102 and the sample hole 106 correspond to each other from top to bottom, and the pressing plate 105 is pressed down until the pressing column 107 covers the plurality of forming holes 102.
  • a plurality of solid culture media 200 are pressed into a plurality of sample wells 106 and pressed on the cover glass 104 to obtain a plurality of microscopic samples in which bacteria/cell suspension droplets are in contact with the cover glass 104, and the samples can be subsequently
  • the pool plate 103 is placed on the microscope platform, and the hanging drop on the solid medium 200 is observed through the cover glass 104 .
  • the microscopic sample preparation device of this embodiment can realize the preparation of multiple solid culture media at one time, complete the tabletting operation of multiple samples at one time, save time, effectively improve the experimental efficiency, simple and convenient operation, and less prone to operational errors , not easy to crush the coverslip.
  • the height of the pressing column 107 should not be smaller than the height of the forming hole 102 , so as to press the solid culture medium 200 out of the forming hole 102 and into the sample hole 106 .
  • the aperture of the sample hole 106 is larger than the aperture of the forming hole 102, so that when the solid medium 200 is pressed into the sample hole 106, the edge of the solid medium 200 is prevented from contacting the hole wall of the sample hole 106 and damaged, and in addition, it is prevented from being damaged. There is trapped gas under the solid medium 200 pressed into the sample hole 106 to cause pressure suppression.
  • the forming plate 101, the sample pool plate 103 and the pressing plate 105 can all be square plates, and the length dimensions of the forming plate 101, the sample pool plate 103 and the pressing plate 105 can be Same, the width dimension can be the same.
  • a plurality of forming holes 102 on the forming plate 101 can be arranged in multiple rows and columns, such as being arranged in 8 rows and 12 columns, that is, the number of forming holes 102 can be 96, the arrangement and quantity of sample holes 106, the number of pressing columns 107 The arrangement and quantity are consistent with the arrangement and quantity of the forming holes 102, so as to form a one-to-one correspondence.
  • hole position marks can be set on the two vertical sides of the forming board 101, such as marking with numbers (such as 1 to 12) in the length direction and marking with letters (such as A ⁇ H), in order to describe the position of each forming hole 102, that is, to describe the positions of multiple samples.
  • positioning pin installation holes 109 can be respectively provided at two opposite sides of the sample cell plate 103 to fix the positioning pin 144, correspondingly, as shown in Fig. 2, Fig. 20 and Fig. 21
  • positioning holes 110 can be respectively provided at the two opposite side plates of the forming plate 101 to be inserted and positioned with the positioning pins 144, so that in the tablet pressing step of step S300, the alignment of the sample cell plate 103 and the forming plate 101 The relative position is positioned so that the two are centered and aligned.
  • the sample preparation assembly can also include a first glass slide 111, a choke ring 112 and a second glass slide 113, for example, the thickness of the first glass slide 111 and the second glass slide 113 is less than 0.2mm ultra-thin glass, the first glass sheet 111 is used to be placed on the bottom surface of the forming plate 101 to close the lower end of the forming hole 102, and the choke ring 112 is used to be placed on the top surface of the forming plate 101, that is, the choke ring 112 Around the outside of a plurality of forming holes 102, together with the top surface of the forming plate 101, it forms a pouring groove for pouring liquid culture medium, and the pouring groove communicates with a plurality of forming holes 102; the second glass sheet 113 is used for removing The choke ring 112 is placed on the top surface of the forming plate 101 to form the sample together with the first glass plate and the forming hole 102 to ensure that the surface of the solid
  • step S100 When using the microscopic sample preparation device of this embodiment to prepare samples, in the step of preparing a solid medium in step S100, it may specifically include:
  • Step S110 place the first glass sheet 111 on the bottom surface of the forming plate 101, and place the choke ring 112 on the top surface of the forming plate 101;
  • Step S120 pour the liquid culture medium into the pouring groove inside the choke ring 112 (such as using a large-volume pipette gun), and the liquid culture medium flows into the plurality of forming holes 102 from the pouring groove, wherein the injection amount of the liquid culture medium It is necessary to ensure that after the plurality of forming holes 102 are filled with liquid medium, the liquid level of the liquid medium is still higher than the top surface of the forming plate 101. After pouring is completed, observe whether each forming hole 102 is filled with liquid medium. If it is not full, you can gently shake the forming plate 101 to distribute the liquid medium evenly, ensuring that the multiple forming holes 102 are filled with liquid medium;
  • Step S130 After the multiple forming holes 102 are filled with liquid culture medium, remove the choke ring 112 from the top surface of the forming plate 101;
  • Step S140 As shown in Figure 9, slowly put down the second glass sheet 113 attached to one side of the forming plate 101, so that the second glass sheet 113 contacts and infiltrates the liquid culture medium on the top surface of the forming plate 101 to ensure that there is no Bubbles, until the second glass sheet 113 is integrally attached to the top surface of the forming plate 101;
  • Step S150 As shown in Fig. 10 and Fig. 11 , press down the second glass sheet 113 uniformly and forcefully to squeeze out a layer of liquid culture medium remaining between the second glass sheet 113 and the top surface of the forming plate 101 to form There is as little residual liquid medium as possible between the plate 101 and the second glass sheet 113 to ensure that the two ends of the solid medium 200 formed in each forming hole 102 are smooth;
  • Step S160 After the liquid culture medium in the forming hole 102 is cooled and solidified, clean up the residual agar on the edge of the forming plate 101, and then place the first glass sheet 111 and the second glass sheet 113 along the bottom surface of the forming plate 101 and the The top surface is translated and pushed away to obtain a cylindrical solid culture medium 200 with flat ends.
  • the choke ring 112 and the forming plate 101 can be fixed and bonded by magnetic attraction, which is very convenient for disassembly and assembly.
  • at least two opposite sides of the choke ring 112 are provided with first magnet installation holes 114 for installing the first magnet;
  • a second magnet installation hole 115 is provided at the two opposite side edges for installing the second magnet.
  • the second magnet corresponds to the first magnet.
  • side ears (as shown in Fig. 4 and Fig. 8 ) can also be respectively provided at two opposite sides of the choke ring 112, when the choke ring 112 is placed on the top surface of the forming plate 101 At this time, the two side ears are located on the outside of the forming board 101 instead of being attached to the forming board 101 .
  • the shapes of the first glass sheet 111 , the choke ring 112 and the second glass sheet 113 can all be squares consistent with the shape of the forming plate 101 .
  • the microsample preparation device can also include a storage box 116 for supporting and positioning the forming plate 101 when preparing the solid medium 200, and for accommodating the overflow liquid culture medium, the storage box 116 has an open top and is used to place the receiving cavity 117 of the forming board 101, the bottom support frame 118 for supporting the forming board 101, and the outer peripheral limit for the forming board 101.
  • Side limit frame 119 in the example of Fig.
  • bottom support frame 118 and side portion limit frame 119 are all arranged in the storage cavity 117, for example, bottom support frame 118 and side portion limit frame 119 are all ribs,
  • An operation avoidance groove 120 may be provided on the outer peripheral wall of the storage box 116, so as to facilitate putting in components such as the forming board 101 .
  • step S110 when using the microscopic sample preparation device of this embodiment to prepare samples, in step S110, as shown in Figure 6 to Figure 8, the first glass sheet 111, the forming plate 101 and the choke ring 112 can be stacked sequentially from bottom to top
  • the side limit frame 119 limits the horizontal positions of the first glass sheet 111 , the forming plate 101 and the choke ring 112 , so that the three are centered and aligned.
  • step S150 as shown in FIG. 9 , when the second glass sheet 113 is pressed down, the extruded liquid culture medium flows into the storage cavity 117 .
  • the microsample preparation device may further include a pressure ring 121, which is used to press the cover glass 104 onto the surface of the sample cell plate 103 so that the cover glass The glass slide 104 and the surface of the sample pool plate 103 are closely attached.
  • the cover glass 104 can be placed on the bottom surface of the sample cell plate 103, and the pressure ring 121 can be placed on the cover glass 104.
  • the bottom surface is used to fix the cover glass 104 and the sample cell plate 103 through the pressure ring 121 .
  • the pressure ring 121 and the sample cell plate 103 are fixed by magnetic attraction.
  • the material of the pressure ring 121 is a metal that can be attracted by a magnet, and at least two opposite sides of the sample pool plate 103 are provided with a third magnet mounting hole 122 (as shown in Figure 3 ) for installing a third magnet.
  • the third magnet and the pressure ring 121 are magnetically attracted (as shown in Figure 15), so that the cover glass 104 and the sample pool plate 103 Tight fit, easy to disassemble.
  • two limiting ribs 123 can be set on the surface of the sample pool plate 103 for mounting the cover glass 104 , and the two limiting ribs 123 are respectively arranged on two sides of the sample pool plate 103 .
  • the cover glass 104 is limited left and right, and on the other hand, the pressure ring 121 is limited left and right.
  • the microsample preparation device can also include a black sticky pad 143, which is used to stick the cover glass 104 on the surface of the sample cell plate 103, and the sticky pad 143 has A plurality of openings run through two opposite surfaces of the adhesive pad 143 , and when the adhesive pad 143 is pasted on the surface of the sample cell plate 103 , the plurality of openings communicate with the plurality of sample wells 106 in one-to-one correspondence.
  • a black sticky pad 143 which is used to stick the cover glass 104 on the surface of the sample cell plate 103
  • the sticky pad 143 has A plurality of openings run through two opposite surfaces of the adhesive pad 143 , and when the adhesive pad 143 is pasted on the surface of the sample cell plate 103 , the plurality of openings communicate with the plurality of sample wells 106 in one-to-one correspondence.
  • the sticky pad 143 is covered with the sample cell plate 103, which can be well attached to the cover glass 104 and the sample cell plate 103 with a smooth surface.
  • the pad 143 is easily separated from the surface of the cover glass 104 and the surface of the sample cell plate 103 after encountering water, and can be pasted repeatedly.
  • the thickness of the sticky pad 143 should be as thin as possible, and the color is black, so as to ensure that no light leaks between the holes when performing optogenetics experiments.
  • the present invention is not limited thereto, and the cover glass 104 and the sample cell plate 103 can also be fixed by other existing connection methods that are easy to disassemble.
  • the micro-sample preparation device may further include a positioning tray 124, the positioning tray 124 includes an accommodating cavity 125 with an open top for placing the sample cell plate 103, and a holding chamber for supporting the cover glass.
  • the pressure ring 121 is in a suspended state and is located outside the middle support protrusion 126, rather than being connected to the middle support protrusion. 126 fit.
  • the surface of the middle supporting protrusion 126 is a plane, so as to have a good supporting effect on the cover glass 104 .
  • the above-mentioned sample cell assembly can be placed on the middle support protrusion 126 of the positioning tray 124 with the pressure ring 121 facing down (as shown in Figures 18 to 20), and then the forming plate 101 is placed on the top surface of the sample cell plate 103 (as shown in Figure 21 and Figure 22 ) with the front side of the solid medium 200 facing down, at this time, the outer limit protrusion 127 of the positioning tray 124 is opposite to the sample cell assembly and The outer peripheral edge of the forming plate 101 is limited, so that the sample pool plate 103 is aligned with the forming plate 101 to prevent their misalignment. Since the cover glass 104 is supported by the middle support protrusion 126, when the pressing plate 105 is pressed down, the cover glass 104 is not easily deformed and damaged.
  • the microsample preparation device can also include a drying rack 128 for drying the solid medium 200 on the forming plate 101, and the drying rack 128 has at least one left side support block 129 and at least one right side support block 130, the left side support block 129 and the right side support block 130 are arranged at intervals and correspond to each other, and the sides facing each other of the left side support block 129 and the right side support block 130 are respectively provided with a
  • the slot 131 into which the molding board 101 is inserted makes the molding board 101 air in a suspended state on the drying rack 128, preventing the solid medium 200 in the molding hole 102 from contacting with the drying rack 128 and deforming.
  • step S170 may also be included: as shown in Figure 13, the forming plate 101 is placed on the drying rack 128 to Dry the solid medium for 200 g. After the solid medium 200 is basically dried, drop the suspension on the solid medium 200, thereby shortening the drying time in the process of dropping the suspension, preventing a large amount of water from being produced in the subsequent tabletting process, and preventing the microorganisms in the suspension from randomly The water swims away and fails to meet the experimental requirements.
  • the drying rack 128 can have a plurality of left side support blocks 129 and a plurality of right side support blocks 130, and a plurality of left side support blocks 129 are spaced apart in the height direction of the drying rack 128
  • the plurality of right support blocks 130 are arranged at intervals in the height direction of the drying rack 128 , and correspond one-to-one to the plurality of left support blocks 129 in the horizontal direction.
  • the drying rack 128 also includes a lower support plate 132, an upper support plate 133, and a plurality of columns 134 (such as copper columns) connecting the lower support plate 132 and the upper support plate 133, for example
  • the lower support plate 132 and the upper support plate 133 are square hollow frames, and there are four columns 134, which are respectively connected and supported from the four corners of the lower support plate 132 and the upper support plate 133.
  • the left support block 129 and the right side The support block 130 can be fixed on the column 134 .
  • the micro-sample preparation device can also include a moisture-retaining element 135, the moisture-retaining element 135 includes a moisture-retaining plate 136 and a transparent cover plate 137, and the moisture-retaining plate 136 has a penetrating moisture-retaining plate A plurality of through-holes 138 on two opposite surfaces of 136, a plurality of through-holes 138 are in order to communicate with a plurality of sample holes 106 in one-to-one correspondence, a surface of the moisturizing board 136 is provided with a water storage tank 139, a plurality of through-holes 138 and a storage tank
  • the water tank 139 is connected to allow the water vapor in the water storage tank 139 to enter the through hole 138, and the plurality of through holes 138 and the water storage tank 139 are separated by a flow blocking ring wall 140 to limit the water in the water storage tank 139 from flowing into the through hole 138
  • the transparent cover 137 can be
  • the solid medium 200 in the sample pool plate 103 can be moisturized by using the moisturizing member 135, so as to prevent the solid medium 200 from dehydrating and deforming (such as shrinking to the middle part) and separated from the cover glass 104 .
  • step S400 when using the microscopic sample preparation device of this embodiment to prepare samples, it may also include step S400: as shown in Figure 29 and Figure 30, the lower surface of the moisturizing board 136 facing away from the water storage tank 139 is opposite to the back of the sample pool plate 103 The surface of the cover glass 104 is attached and placed, and now the water storage tank 139 is facing upwards, water is added to the water storage tank 139, and then the transparent cover plate 137 is covered on the upper surface of the moisturizing board 136, so that the water storage tank 139 is closed to obtain an airtight In the moisturizing space, the water in the water storage tank 139 evaporates to maintain a certain humidity in the moisturizing space, effectively preventing the solid medium 200 in the sample pool plate 103 from being dehydrated.
  • the transparent cover plate 137 is made of transparent material, it can ensure that the light source above the microscope device is irradiated on the solid medium 200 smoothly, without affecting the normal operation of the optical experiment.
  • the micro-sample preparation device further includes an intermediate plate 141.
  • the intermediate plate 141 is provided with a plurality of through holes 142 corresponding to the plurality of forming holes 102.
  • the intermediate plate 141 The forming plate 101 and the cell culture plate 300 of the cell phenotype control device are detachably connected (as shown in FIG. 32 ), so that the microsample preparation device and the cell phenotype control device constitute a set, which is easy to assemble and disassemble.
  • the multiple forming holes 102 on the forming plate 101, the multiple sample holes 106 of the sample pool plate 103, and the multiple through holes 142 on the connecting plate 141 correspond to the multiple microwells 301 on the cell culture plate 300 .
  • the connecting plate 141 and the forming plate 101 may be connected by magnetic attraction, and the connecting plate 141 and the cell culture plate 300 may be connected by an interference fit.
  • two opposite sides of the connecting plate 141 are provided with magnet installation holes for installing a fourth magnet, and the fourth magnet corresponds to the second magnet of the forming plate 101, so as to connect the connecting plate 141 and the forming plate.
  • the board 101 is magnetically fixed.
  • the embodiment of the second aspect of the present invention provides a cell phenotype control device, as shown in Figure 32 and Figure 33, the cell phenotype control device includes a cell culture plate 300, a light source control box 400 and Describe the microsample preparation setup. Since in the embodiment of the first aspect, the structure of the microsample preparation device has been described in detail, the content thereof is incorporated here, and the description is omitted here.

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Abstract

一种显微制样装置及细胞表型控制装置，包括：成型板（101），具有贯穿成型板（101）的两个相对表面的多个成型孔（102），用于成型多个固体培养基；样品池板（103），具有贯穿样品池板（103）的两个相对表面的多个样品孔（106），用于容纳多个固体培养基；盖玻片（104），用于放置在样品池板（103）的一表面；压板（105），设有多个压柱（107），用于将多个成型孔（102）内的多个固体培养基压入多个样品孔（106）内并压在盖玻片（104）上。可以实现一次制备多个固体培养基，一次性完成多个样品的压片操作，节省时间，有效提高实验效率，不容易出现操作失误的情况，不容易压碎盖玻片。

Description

显微制样装置及细胞表型控制装置 技术领域
本发明涉及显微实验技术领域，尤其是一种显微制样装置及细胞表型控制装置。
背景技术
生物或医学实验过程中，为了更好的在显微镜上观察单个细菌或细胞，经常会采用以下制样流程：将细菌/细胞悬液滴在切好的固体培养基(例如琼脂块)上；等琼脂表面稍微晾干后，将琼脂块的滴有悬液的一面压在玻璃盖玻片上；然后再放置在倒置显微镜上观察细菌或细胞。上述描述的过程可称为压片过程。
上述实验过程需要切割琼脂块、在琼脂块上滴细菌/细胞悬液、将滴有悬液的琼脂块压在玻璃盖玻片上等手工操作，实验人员必须小心谨慎的操作，才能得到效果较好的制片样品，比如可能压碎玻璃盖玻片，另外，在对大量实验样品进行压片时，需要逐一操作，整个实验过程耗时长，效率低，耗费实验人员的大量时间和精力，而且容易出现操作失误的情况，严重影响了科研或医学诊断的进度。
发明内容
为了解决上述背景技术指出的问题，本发明实施例提供一种显微制样装置及细胞表型控制装置。
根据本发明实施例的一方面，提供一种显微制样装置，包括：成型板，具有贯穿所述成型板的两个相对表面的多个成型孔，用于成型多个固体培养基；样品池板，具有贯穿所述样品池板的两个相对表面的多个样品孔，用于容纳多个所述固体培养基；盖玻片，用于放置在所述样品池板的一表面；压板，设有多个压柱，用于将多个所述成型孔内的多个固体培养基压入多个所述样品孔内并压在所述盖玻片上。
根据本发明实施例的另一方面，提供一种细胞表型控制装置，包括细胞培养板和前述实施例的显微制样装置。
本发明实施例的有益效果至少包括：
1、本发明实施例通过设置具有多个成型孔的成型板、具有多个样品孔的样品池板和具有多个压柱的压板，可以实现一次制备多个固体培养基，一次性完成多个样品的压 片操作，节省时间，有效提高实验效率，操作简单、方便、快捷，不容易出现操作失误的情况，不容易压碎盖玻片；
2、本发明实施例通过设置第一玻璃片和第二玻璃片与成型板共同成型固体培养基，保证固体培养基的表面平整，为滴悬液提供表面质量较高的培养基；
3、本发明实施例通过设置收纳盒，既可以收纳液体培养基，又可以在制备固体培养基时对成型板起到良好的支撑效果，还对成型板、第一玻璃片和第二玻璃片起到定位对正作用；
4、本发明实施例通过设置晾干架将固体培养基在滴悬液之前基本晾干，缩短滴悬液过程中的晾干时间，防止后续压片过程中产生大量的水，进而避免悬液中微生物随水游走而达不到实验要求；
5、本发明实施例通过设置保湿件对样品池板内的固体培养基进行保湿，防止固体培养基因脱水变形而与盖玻片分离，便于实验人员长时间进行显微观察；
6、本发明实施例通过设置中接板将成型板与细胞培养板可拆卸连接，形成一套便于拆装的细胞表型控制装置，便于进行光遗传学实验。
参照后文的说明和附图，详细公开了本发明的特定实施方式，指明了本发明的原理可以被采用的方式。应该理解，本发明的实施方式在范围上并不因而受到限制。在所附权利要求的精神和条款的范围内，本发明的实施方式包括许多改变、修改和等同。
附图说明
以下附图仅旨在于对本发明做示意性说明和解释，并不限定本发明的范围。其中：
所包括的附图用来提供对本发明实施例的进一步的理解，其构成了说明书的一部分，用于例示本发明的实施方式，并与文字描述一起来阐述本发明的原理。显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。在附图中：
图1是本发明实施例的显微制样装置的部分部件的一个示例的示意图；
图2是本发明实施例的显微制样装置的成型板的一个示例的示意图；
图3是本发明实施例的显微制样装置的样品池板的一个示例的示意图；
图4是本发明实施例的显微制样装置的阻流圈的一个示例的示意图；
图5是本发明实施例的显微制样装置的收纳盒的一个示例的示意图；
图6是本发明实施例的显微制样装置的制样组件的一个示例的示意图；
图7至图10是本发明实施例的显微制样装置在制样过程中的部件配合示意图；
图11是图10中组件的剖视图；
图12和图13是本发明实施例的显微制样装置的晾干架的一个示例的示意图；
图14至图16是本发明实施例的显微制样装置的样品池板组件的一个示例的示意图；
图17是本发明实施例的显微制样装置的粘性垫放置于样品池板与盖玻片之间的示意图；
图18是本发明实施例的显微制样装置的定位托盘的一个示例的示意图；
图19和图20是本发明实施例的显微制样装置的样品池板组件放置于定位托盘的示意图；
图21和图22是本发明实施例的显微制样装置的样品池板组件和成型板放置于定位托盘的示意图；
图23是本发明实施例的显微制样装置的压板的一个示例的示意图；
图24和图25是本发明实施例的显微制样装置在压片过程中的部件配合示意图；
图26和图27是本发明实施例的显微制样装置的在压片后的部件配合示意图；
图28是本发明实施例的显微制样装置的保湿件的一个示例的示意图；
图29和图30是本发明实施例的显微制样装置的保湿件与样品池板配合的示意图；
图31是本发明实施例的显微制样装置的中接板的一个示例的示意图；
图32和图33是本发明实施例的细胞表型控制装置的一个示例的示意图。
具体实施方式
为了对本发明的技术特征、目的和效果有更加清楚的理解，现对照附图说明本发明的具体实施方式。在本发明的描述中，除非另有说明，“多个”的含义是两个或两个以上。
参照附图，通过下面的说明书，本发明的前述以及其它特征将变得明显。在说明书和附图中，具体公开了本发明的特定实施方式，其表明了其中可以采用本发明的原则的部分实施方式，应了解的是，本发明并不限于所描述的实施方式，相反，本发明包括落入所附权利要求的范围内的全部修改、变型以及等同物。
在本发明实施例中，术语“第一”、“第二”等用于对不同元素从称为上进行区分，但并不表示这些元素的空间排列或时间顺序等，这些元素不应被这些术语所限制。术语 “和/或”包括相关联列出的术语的一种或多个中的任何一个和所有组合。术语“包含”、“包括”、“具有”等是指所陈述的特征、元素、元件或组件的存在，但并不排除存在或添加一个或多个其它特征、元素、元件或组件。
在本发明实施例中，单数形式“一”、“该”等可以包括复数形式，应广义地理解为“一种”或“一类”而并不是限定为“一个”的含义；此外术语“所述”应理解为既包括单数形式也包括复数形式，除非上下文另外明确地指出；此外术语“根据”应理解为“至少部分根据……”，术语“基于”应理解为“至少部分基于……”，除非上下文另外明确指出；此外术语“多个”的含义是两个或两个以上，除非另有说明。
在本发明实施例中，形容词性或副词性修饰语“上”和“下”、“顶”和“底”、“内”和“外”、“正面”和“背面”的使用仅是为了便于多组术语之间的相对参考，且并非描述对经修饰术语的任何特定的方向限制。
下面参照附图对本发明实施例的实施方式进行说明。
第一方面的实施例
本发明第一方面的实施例提供一种显微制样装置。
本发明实施例的显微制样装置包括制样组件和压片组件，如图1所示，制样组件包括成型板101，成型板101具有分别贯穿成型板101的两个相对表面的多个成型孔102，多个成型孔102用以成型多个固体培养基200(如图11所示)，实现单次制备多个固体培养基200，例如固体培养基200为琼脂块；如图14、图23所示，压片组件包括样品池板103、盖玻片104和压板105，样品池板103具有贯穿样品池板103的两个相对表面的多个样品孔106，多个样品孔106能与多个成型孔102一一对应，盖玻片104用于放置在样品池板103的一表面，压板105设有多个压柱107，多个压柱107能与多个成型孔102一一对应，用以将多个成型孔102内的多个固体培养基200压入多个样品孔106内并压在盖玻片104上，例如压板105上还设有把手108，把手108和压柱107分别设于压板105的相对两侧面上，以便于手持把手108下压压板105。
采用本实施例的显微制样装置制样，可以包括以下步骤：
步骤S100：制备固体培养基(例如琼脂块)：如图11所示，将液体培养基(例如琼脂液)浇注到成型板101的多个成型孔102内，液体培养基冷却凝固后成为固体培养基200；
步骤S200：滴液：将细菌/细胞悬液滴在多个固体培养基200的表面(称为正面)；
步骤S300：压片：如图24至图27所示，将盖玻片104置于样品池板103的底面， 将成型板101置于样品池板103的顶面(固体培养基200的正面朝下)，将压板105置于成型板101上，此时压柱107、成型孔102和样品孔106由上至下一一对应，下压压板105，直至压柱107将多个成型孔102内的多个固体培养基200压入多个样品孔106内并压在盖玻片104上，即可得到细菌/细胞悬液滴与盖玻片104接触的多个显微样品，后续可以将样品池板103放置在显微镜平台上，透过盖玻片104对固体培养基200上的悬滴液进行观察。
采用本实施例的显微制样装置可以实现一次制备多个固体培养基，一次性完成多个样品的压片操作，节省时间，有效提高实验效率，操作简单方便，不容易出现操作失误的情况，不容易压碎盖玻片。
进一步，压柱107的高度不应小于成型孔102的高度，以将固体培养基200从成型孔102压出，压入样品孔106。
进一步，样品孔106的孔径大于成型孔102的孔径，以在固体培养基200被压入样品孔106内时，防止固体培养基200的边缘与样品孔106的孔壁接触而破损，另外还防止被压入样品孔106内的固体培养基200下方有存留气体造成憋压。
在一些实施例中，如图2、图3和图23所示，成型板101、样品池板103和压板105可以均为方形板，成型板101、样品池板103和压板105的长度尺寸可以相同，宽度尺寸可以相同。成型板101上的多个成型孔102可以排列为多行多列，比如排列为8行12列，即成型孔102的数量可以是96个，样品孔106的排列方式和数量、压柱107的排列方式和数量均与成型孔102的排列方式和数量一致，以便形成一一对应关系。
进一步，如图2所示，可以在成型板101的两个垂直侧边处设置孔位标记，比如长度方向上以数字字符标记(比如1～12)，宽度方向上以字母字符标记(比如A～H)，以便于描述各成型孔102的位置，亦即便于描述多个样品的位置。
进一步，如图3、图20所示，在样品池板103的两个相对侧边处可以分别设置定位销安装孔109，以固定定位销144，相应地，如图2、图20、图21所示，在成型板101的两个相对侧板处可以分别设置定位孔110，以与定位销144插接定位，从而在步骤S300的压片步骤中，对样品池板103和成型板101的相对位置进行定位，使二者居中对正。
在一些实施例中，如图6所示，制样组件还可以包括第一玻璃片111、阻流圈112和第二玻璃片113，例如第一玻璃片111和第二玻璃片113是厚度小于0.2毫米的超薄玻璃，第一玻璃片111用于放置在成型板101的底面，以封闭成型孔102的下端，阻流圈112用于放置在成型板101的顶面，即阻流圈112围绕在多个成型孔102外侧，以与 成型板101的顶面共同围成用于浇注液体培养基的浇注槽，浇注槽与多个成型孔102连通；第二玻璃片113用于在移除阻流圈112后放置在成型板101的顶面，以与第一玻璃板和成型孔102共同成型样品，保证固体培养基的表面平整，为滴悬液提供表面质量较高的培养基。
采用本实施例的显微制样装置制样时，在步骤S100的制备固体培养基步骤中，具体可以包括：
步骤S110：如图7、图8所示，将第一玻璃片111置于成型板101的底面，将阻流圈112置于成型板101顶面；
步骤S120：将液体培养基浇注到阻流圈112内侧的浇注槽内(比如采用大容积的移液枪)，液体培养基由浇注槽流入多个成型孔102内，其中液体培养基的注入量需保证多个成型孔102内充满液体培养基后，液体培养基的液面仍高于成型板101的顶面，待浇注完成后，观察每个成型孔102内是否充满液体培养基，若存在未充满的情况，可以轻轻晃动成型板101，使液体培养基均匀分布，保证多个成型孔102内都充满液体培养基；
步骤S130：待多个成型孔102内都充满液体培养基后，将阻流圈112从成型板101的顶面上取走；
步骤S140：如图9所示，将第二玻璃片113贴合着成型板101的一侧边缓慢放下，使第二玻璃片113接触并浸润成型板101顶面的液体培养基，确保不存在气泡，直至第二玻璃片113整体贴合在成型板101的顶面；
步骤S150：如图10、图11所示，均匀用力下压第二玻璃片113，以将第二玻璃片113与成型板101的顶面之间残留的一层液体培养基挤出，使成型板101与第二玻璃片113之间尽可能少的残留液体培养基，以保证各成型孔102内成型的固体培养基200的两端面平整；
步骤S160：待成型孔102内的液体培养基冷却凝固后，将成型板101边缘的残留的琼脂清理干净，再将第一玻璃片111和第二玻璃片113分别沿着成型板101的底面和顶面平移推走，即得到两端面平整的柱形的固体培养基200。
进一步，阻流圈112与成型板101可以通过磁吸的方式固定贴合，拆装非常方便。例如，具体是，如图4所示，阻流圈112的至少两个相对侧边设有第一磁铁安装孔114，用于安装第一磁铁；如图2所示，成型板101的至少两个相对侧缘处设有第二磁铁安装孔115，用于安装第二磁铁，第二磁铁与第一磁铁对应，当阻流圈112置于成型板101 的顶面时，第一磁铁和第二磁铁磁吸，以使阻流圈112和成型板101贴合固定，磁铁和磁铁安装孔可以通过过盈配合的方式固定。
为了便于取放阻流圈112，还可以在阻流圈112的两个相对侧边处分别设置侧耳(如图4、图8所示)，当阻流圈112置于成型板101的顶面时，两个侧耳位于成型板101的外侧，而非与成型板101贴合。
进一步，第一玻璃片111、阻流圈112和第二玻璃片113的形状均可以是与成型板101形状一致的方形。
在一些实施例中，如图5所示，显微制样装置还可以包括收纳盒116，用于在制备固体培养基200时对成型板101起到支撑、定位作用，以及用于收纳溢流的液体培养基，收纳盒116内部具有顶部敞口且用于放置成型板101的收纳腔117、用于支撑成型板101的底部支撑架118、以及用于对成型板101的外周缘限位的侧部限位架119，在图5的示例中，底部支撑架118和侧部限位架119均设于收纳腔117内，例如底部支撑架118和侧部限位架119均为筋板，可以在收纳盒116的外周壁上设置操作避让槽120，以便于放入成型板101等部件。
采用本实施例的显微制样装置制样时，在步骤S110中，如图6至图8所示，可以将第一玻璃片111、成型板101和阻流圈112由下至上依次叠置于收纳腔117内的底部支撑架118上，侧部限位架119限制第一玻璃片111、成型板101和阻流圈112的水平位置，使三者居中对正。在步骤S150中，如图9所示，当下压第二玻璃片113时，被挤出的液体培养基流入收纳腔117内。
在一些实施例中，如图14至图16所示，显微制样装置还可以包括压环121，压环121用于将盖玻片104压紧在样品池板103的表面，以使盖玻片104和样品池板103的表面紧密贴合。
采用本实施例的显微制样装置制样时，在步骤S300的压片步骤中，可以将盖玻片104置于样品池板103的底面后，将压环121置于盖玻片104的底面，以通过压环121将盖玻片104和样品池板103固定。
在一种可行的技术方案中，压环121与样品池板103通过磁吸的方式固定。具体是，压环121的材质为能被磁铁吸引的金属，样品池板103的至少两个相对侧边处设有第三磁铁安装孔122(如图3所示)，用于安装第三磁铁，当压环121置于盖玻片104的背对样品池板103的表面时，第三磁铁和压环121磁吸(如图15所示)，以使盖玻片104和样品池板103紧密贴合，拆装方便。
进一步，如图3所示，在样品池板103的用于安装盖玻片104的表面可以设置两个限位凸棱123，两个限位凸棱123分别设于样品池板103的两个相对侧边处，一方面对盖玻片104进行左右限位，另一方面对压环121进行左右限位。
在另一些实施例中，如图17所示，显微制样装置还可以包括黑色的粘性垫143，粘性垫143用于将盖玻片104粘贴于样品池板103的表面，粘性垫143具有贯穿粘性垫143的两个相对表面的多个开孔，当粘性垫143粘贴于样品池板103的表面时，多个开孔与多个样品孔106一一对应连通。
本实施例与前述实施例中压环固定的方式相比，粘性垫143布满样品池板103，能够很好地与盖玻片104和表面光滑的样品池板103紧密贴合在一起，粘性垫143在遇到水之后很容易与盖玻片104的表面和样品池板103的表面分离，可以反复黏贴。粘性垫143的厚度应尽可能的薄，颜色为黑色，在进行光遗传学实验时，能够保证孔之间不漏光。
但本发明并不以此为限，盖玻片104与样品池板103还可以通过其它现有的便于拆装的连接方式固定。
在一些实施例中，如图18所示，显微制样装置还可以包括定位托盘124，定位托盘124包括顶部敞口且用于放置样品池板103的容纳腔125、用于支撑盖玻片104和样品池板103的中部支撑凸起126、以及用于对盖玻片104和样品池板103的外周缘限位的外侧限位凸起127，当盖玻片104、样品池板103和压环121构成的样品池组件放置在中部支撑凸起126上时(如图19、图20所示)，压环121呈悬空状态位于中部支撑凸起126的外侧，而非与中部支撑凸起126贴合。其中中部支撑凸起126的表面为平面，以对盖玻片104起到良好支撑效果。
在步骤S300的压片步骤中，可以将上述样品池组件以压环121朝下的状态置于定位托盘124的中部支撑凸起126上(如图18至图20所示)，再将成型板101以固体培养基200的正面朝下的状态置于样品池板103的顶面上(如图21、图22所示)，此时定位托盘124的外侧限位凸起127对样品池组件和成型板101的外周缘限位，使样品池板103与成型板101对正，防止二者错位。由于盖玻片104由中部支撑凸起126支撑，当压板105下压时，盖玻片104不容易发生变形损坏。
在一些实施例中，如图12所示，显微制样装置还可以包括晾干架128，用于晾干成型板101上的固体培养基200，晾干架128具有至少一个左侧支撑块129和至少一个右侧支撑块130，左侧支撑块129和右侧支撑块130左右间隔设置且一一对应，左侧支撑 块129和右侧支撑块130的朝向彼此的一侧分别设有供成型板101插入的插槽131，以使成型板101呈悬空状态晾晒于晾干架128上，防止成型孔102内的固体培养基200与晾干架128接触而发生变形。
采用本实施例的显微制样装置制样时，在步骤S100的制备固体培养基步骤中，还可以包括步骤S170：如图13所示，将成型板101放置于晾干架128上，以晾干固体培养基200。待固体培养基200基本晾干后，在固体培养基200上滴悬液，从而缩短滴悬液过程中的晾干时间，防止后续压片过程中产生大量的水，进而避免悬液中微生物随水游走而达不到实验要求。
进一步，如图12、图13所示，晾干架128可以具有多个左侧支撑块129和多个右侧支撑块130，多个左侧支撑块129在晾干架128的高度方向上间隔设置，相应地，多个右侧支撑块130在晾干架128的高度方向上间隔设置，且在水平方向上与多个左侧支撑块129一一对应。
进一步，如图12、图13所示，晾干架128还包括下部支撑板132、上部支撑板133、以及连接下部支撑板132和上部支撑板133的多个立柱134(比如铜柱)，例如下部支撑板132和上部支撑板133均为方形镂空架，立柱134为四个，分别从下部支撑板132和上部支撑板133的四个角处进行连接和支撑，左侧支撑块129和右侧支撑块130可以固定在立柱134上。
在一些实施例中，如图28、图29、图30所示，显微制样装置还可以包括保湿件135，保湿件135包括保湿板136和透明盖板137，保湿板136具有贯穿保湿板136的两个相对表面的多个透孔138，多个透孔138用以与多个样品孔106一一对应连通，保湿板136的一表面设有蓄水槽139，多个透孔138和蓄水槽139连通，以允许蓄水槽139内的水蒸气进入透孔138，多个透孔138与蓄水槽139之间通过阻流环壁140隔开，以限制蓄水槽139内的水流入透孔138；透明盖板137可以是玻璃盖板，用于盖在保湿板136的表面，以封闭蓄水槽139。
在实验过程中，当需要对显微样品进行长时间观察时，可以采用保湿件135对样品池板103内的固体培养基200进行保湿，防止固体培养基200因脱水变形(比如向中部萎缩)而与盖玻片104分离。
采用本实施例的显微制样装置制样时，还可以包括步骤S400：如图29、图30所示，将保湿板136的背对蓄水槽139的下表面与样品池板103的背对盖玻片104的表面贴合放置，此时蓄水槽139朝上，向蓄水槽139内加水，再将透明盖板137盖在保湿板136 的上表面，以将蓄水槽139封闭，得到密闭的保湿空间，蓄水槽139内的水分蒸发，使保湿空间内保持一定湿度，有效防止样品池板103中的固体培养基200发生脱水。
另外，由于多个透孔138与多个样品孔106一一对应，且透明盖板137为透明材质，能够保证显微镜装置上方的光源顺利照射在固体培养基200上，不影响光学实验正常进行。
在一些实施例中，如图31所示，显微制样装置还包括中接板141，中接板141设有与多个成型孔102一一对应的多个通孔142，中接板141用于将成型板101和细胞表型控制装置的细胞培养板300可拆卸连接(如图32所示)，以使显微制样装置和细胞表型控制装置构成一套装置，拆装方便。当成型板101和细胞培养板300通过中接板141连接时，成型板101上的多个成型孔102、样品池板103的多个样品孔106、中接板141上的多个通孔142和细胞培养板300上的多个微孔301一一对应。
其中，中接板141与成型板101可以通过磁吸的方式连接，中接板141与细胞培养板300可以通过过盈配合的方式连接。例如，具体是，中接板141的两个相对侧边处设有磁铁安装孔，用于安装第四磁铁，第四磁铁与成型板101的第二磁铁对应，以将中接板141和成型板101磁吸固定。
第二方面的实施例
本发明第二方面的实施例提供一种细胞表型控制装置，如图32、图33所示，该细胞表型控制装置包括细胞培养板300、光源控制盒400和第一方面的实施例所描述的显微制样装置。由于在第一方面的实施例中，已经对该显微制样装置的结构进行了详细说明，其内容被合并于此，此处省略说明。
在本发明实施例中，关于该细胞表型控制装置的其它结构，可以参考相关技术，此处省略说明。
以上结合具体的实施方式对本发明进行了描述，但本领域技术人员应该清楚，这些描述都是示例性的，并不是对本发明保护范围的限制。本领域技术人员可以根据本发明的精神和原理对本发明做出各种变型和修改，这些变型和修改也在本发明的范围内。
以上参照附图描述了本发明的优选实施方式。这些实施方式的许多特征和优点根据该详细的说明书是清楚的，因此所附权利要求旨在覆盖这些实施方式的落入其真实精神和范围内的所有这些特征和优点。此外，由于本领域的技术人员容易想到很多修改和改变，因此不是要将本发明的实施方式限于所例示和描述的精确结构和操作，而是可以涵盖落入其范围内的所有合适修改和等同物。

Claims (12)

  1. 一种显微制样装置，其特征在于，包括：
    成型板，具有贯穿所述成型板的两个相对表面的多个成型孔，用于成型多个固体培养基；
    样品池板，具有贯穿所述样品池板的两个相对表面的多个样品孔，用于容纳多个所述固体培养基；
    盖玻片，用于放置在所述样品池板的一表面；
    压板，设有多个压柱，用于将多个所述成型孔内的多个固体培养基压入多个所述样品孔内并压在所述盖玻片上。
  2. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    第一玻璃片，用于放置在所述成型板的底面；
    阻流圈，用于放置在所述成型板的顶面，以与所述成型板的顶面共同围成用于浇注液体培养基的浇注槽，所述浇注槽与多个所述成型孔连通；
    第二玻璃片，用于在移除所述阻流圈后放置在所述成型板的顶面，以与所述第一玻璃板和所述成型孔共同成型所述固体培养基。
  3. 如权利要求2所述的显微制样装置，其特征在于，所述显微制样装置还包括：
    收纳盒，包括顶部敞口且用于放置所述成型板的收纳腔、用于支撑所述成型板的底部支撑架、以及用于对所述成型板的外周缘限位的侧部限位架。
  4. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    压环，用于将所述盖玻片压紧在所述样品池板的表面。
  5. 如权利要求4所述的显微制样装置，其特征在于，所述压环与所述样品池板通过磁吸的方式固定。
  6. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    黑色的粘性垫，用于将所述盖玻片粘贴于所述样品池板的表面，所述粘性垫具有贯穿所述粘性垫的两个相对表面的多个开孔，多个所述开孔用于与多个所述样品孔一一对应。
  7. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    定位托盘，包括顶部敞口且用于放置所述样品池板的容纳腔、用于支撑所述盖玻片和所述样品池板的中部支撑凸起、以及用于对所述盖玻片和所述样品池板的外周缘限位的外侧限位凸起。
  8. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    晾干架，具有至少一个左侧支撑块和至少一个右侧支撑块，所述左侧支撑块和右侧支撑块左右间隔设置且一一对应，所述左侧支撑块和所述右侧支撑块的朝向彼此的一侧分别设有供所述成型板插入的插槽。
  9. 如权利要求1所述的显微制样装置，其特征在于，所述压板上还设有把手，所述把手和所述压柱分别设于所述压板的两个相对表面上。
  10. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    保湿板，设有多个透孔，用于与多个所述样品孔一一对应连通，所述保湿板的一表面设有蓄水槽，多个所述透孔和所述蓄水槽连通，多个所述透孔与所述蓄水槽之间通过阻流环壁隔开；
    透明盖板，用于盖在所述保湿板的表面，以封闭所述蓄水槽。
  11. 如权利要求1所述的显微制样装置，其特征在于，还包括：
    中接板，设有用于与多个成型孔一一对应的多个通孔，所述中接板用于将所述成型板和细胞表型控制装置的细胞培养板可拆卸连接。
  12. 一种细胞表型控制装置，其特征在于，包括如权利要求1至11任一项所述的显微制样装置和细胞培养板。
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CN101126688A (zh) * 2007-09-11 2008-02-20 深圳职业技术学院 一种规模化制备细胞显微样品的方法
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