WO2022190605A1 - 自動分析装置の制御方法 - Google Patents
自動分析装置の制御方法 Download PDFInfo
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- WO2022190605A1 WO2022190605A1 PCT/JP2022/000207 JP2022000207W WO2022190605A1 WO 2022190605 A1 WO2022190605 A1 WO 2022190605A1 JP 2022000207 W JP2022000207 W JP 2022000207W WO 2022190605 A1 WO2022190605 A1 WO 2022190605A1
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- 238000004458 analytical method Methods 0.000 title claims abstract description 291
- 238000000034 method Methods 0.000 title claims abstract description 144
- 238000000926 separation method Methods 0.000 claims abstract description 222
- 239000002904 solvent Substances 0.000 claims abstract description 188
- 230000008569 process Effects 0.000 claims abstract description 70
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 238000001514 detection method Methods 0.000 claims description 22
- 238000011282 treatment Methods 0.000 claims description 7
- 230000006866 deterioration Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 57
- 238000010586 diagram Methods 0.000 description 10
- 239000012472 biological sample Substances 0.000 description 3
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- 230000007246 mechanism Effects 0.000 description 3
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- 239000000203 mixture Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
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- G01N35/00584—Control arrangements for automatic analysers
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- G01N2035/0094—Scheduling optimisation; experiment design
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
- G01N30/20—Injection using a sampling valve
Definitions
- the present invention relates to a method of controlling an automatic analyzer for quantitative or qualitative analysis of biological samples such as blood and urine.
- Some automated analyzers use a chromatograph to separate the analyte component from the biological sample.
- Chromatography involves passing a sample together with a mobile phase (solvent) through a separation column packed with a stationary phase, thereby separating the components to be analyzed through interactions between the components in the sample and the stationary and mobile phases. It is an analyzer.
- the separated components can be detected by a detector to identify the components of the sample.
- a chromatograph using a liquid mobile phase is called a liquid chromatograph.
- the liquid chromatograph unit is provided with multiple analysis channels each having a column in parallel, and these are connected to a single detector to improve the operating rate of the detector and increase the throughput.
- an improved automatic analyzer In an automatic analyzer having such a plurality of analysis channels in parallel, it is necessary to select an analysis channel for executing separation processing for each analysis when creating an analysis schedule.
- a liquid chromatograph has at least a column for separating the components to be analyzed and a liquid delivery device for injecting liquid into the column in one analysis channel.
- Patent Document 1 proposes a technique for creating an analysis schedule in which the order of analysis is changed so that multiple samples are analyzed continuously under the same separation conditions.
- the time required for analysis is shortened by reducing the number of times the analysis channel is washed (including solvent replacement).
- An object of the present invention is to solve the above-mentioned problems, and in a control method and automatic analyzer for an automatic analyzer having a liquid chromatograph equipped with a plurality of analysis channels in parallel, decrease in throughput and solvent consumption due to solvent replacement
- An object of the present invention is to provide a control method and an automatic analyzer that avoid an increase in the
- the present invention is configured as follows in order to achieve the above objectives.
- a liquid chromatograph having a pretreatment part for pretreatment of a sample and a plurality of analysis channels arranged in parallel with each other separates the sample treated in the pretreatment part using a plurality of types of solvents.
- a separation unit that performs a separation process, a detection unit that detects the sample separated by the separation unit, and a control unit, wherein the control unit creates an analysis schedule before executing analysis, and analyzes the sample.
- the method for controlling an automatic analyzer it is determined which of the plurality of analysis channels should be used to introduce a plurality of separation treatments using solvents of different types with a time difference from each other,
- the solvent used in the next separation process which is performed at the timing after the start of the plurality of separation processes in which it is determined whether to introduce them with a time lag, and each of the solvents used in the plurality of separation processes to determine whether or not the solvent replacement process is necessary for the analysis channels used for the plurality of separation processes, and according to the determination, for the next separation process among the plurality of analysis channels
- the analysis flow path to be used is determined, and the analysis schedule is created.
- a liquid chromatograph having a pretreatment part for pretreatment of a sample and a plurality of analysis channels arranged in parallel with each other separates the sample treated in the pretreatment part using a plurality of types of solvents.
- a separation unit that performs separation processing, a detection unit that detects the sample separated by the separation unit, and a control unit, wherein the control unit creates an analysis schedule before executing the analysis, and the created analysis
- the control unit performs a plurality of separation processes using solvents of different types using any one of the plurality of analysis channels at different time intervals.
- the solvent used in the next separation treatment which is performed at the timing after the start of the plurality of separation treatments in which it was decided whether to introduce with a time difference from each other, and the plurality of comparing each of the solvents used in the separation process, determining whether or not the solvent replacement process is required for the analysis channels used in the plurality of separation processes, and determining whether the solvent replacement process is required; According to the determination of whether or not, the analysis channel to be used for the next separation process among the plurality of analysis channels is determined, and the analysis schedule is created.
- a control method for an automatic analyzer having a liquid chromatograph equipped with a plurality of analysis channels in parallel and a control method for avoiding a decrease in throughput and an increase in solvent consumption due to solvent replacement in the automatic analyzer.
- an automatic analyzer can be provided.
- FIG. 1 is a schematic diagram showing the configuration of an automatic analyzer to which the present invention is applied;
- FIG. 4 is a flow chart showing the processing procedure of scheduling of the entire automatic analyzer in the example.
- FIG. 3 is a flow chart showing in more detail a scheduling processing procedure in the demultiplexer in FIG. 2;
- FIG. 4 is a schematic diagram of scheduling in Example 1.
- FIG. FIG. 10 is a schematic diagram of scheduling in Example 2;
- FIG. 10 is a flow chart showing in more detail the scheduling processing procedure in the demultiplexer in FIG. 2 when the scheduling method according to the third embodiment can be selected from two methods;
- FIG. FIG. 11 is a schematic diagram of scheduling in Example 3;
- liquid chromatograph is used as a chromatograph in the examples of the present invention
- present invention can also be applied using other chromatographs, such as gas chromatographs.
- a pretreatment unit 101 for pretreatment of a sample and a chromatograph having a plurality of analysis flow paths 105 to 107 arranged in parallel with each other are processed in the pretreatment unit 101.
- a separation unit 102 that performs a separation process of separating a sample using a plurality of types of solvents
- a detection unit 103 that detects the sample separated by the separation unit 102
- a pretreatment unit 102 that a separation unit 102, and a detection unit 103
- a control unit 104 that controls the control unit 104 to create an analysis schedule before executing analysis, and to analyze a sample based on the created analysis schedule. .
- the time difference is set.
- the solvent used in the next separation treatment which is performed at the timing after the start of the plurality of separation treatments determined to be introduced, and the solvent used in the plurality of separation treatments decided to be introduced with a time difference from each other. , and determines whether solvent replacement processing is necessary for the analysis channels 105 to 107 used for multiple separation processing. Then, according to the determination as to whether or not the solvent replacement process is necessary, the analysis flow paths 105 to 107 to be used for the next separation process among the plurality of analysis flow paths 105 to 107 are determined, and an analysis schedule is created.
- FIG. 1 is a schematic diagram showing the configuration of an automatic analyzer to which the present invention is applied.
- the automatic analyzer has a pretreatment unit 101 for pretreatment of a sample, and a liquid chromatograph having a plurality of parallel analysis channels to separate the sample treated in the pretreatment unit 101.
- a separation unit 102 a detection unit 103 for detecting the sample separated by the separation unit 102 , and a control unit 104 for controlling the pretreatment unit 101 , the separation unit 102 and the detection unit 103 are provided.
- the input/output device 126 displays information and inputs commands from the user to the control unit 104 .
- the separation unit 102 includes an analysis channel 105, an analysis channel 106, and an analysis channel 107 arranged in parallel, and a valve for switching the analysis channel through which the sample processed in the pretreatment unit 101 is introduced. 108, a valve 109 for switching analysis channels connected to the detection unit 103, and tanks 110, 111, 112, and 113 each containing different solvents.
- the analysis channel 105 includes an analysis column 114, a liquid delivery device 115 that delivers a solvent to the analysis column 114 at high pressure, an injection valve 116 that is connected to the liquid delivery device 115 and introduces a sample into the analysis column 114, and a liquid delivery method. and a valve 117 for switching the solvent introduced into the device 115 .
- Valve 117 is connected to solvent tank 110, solvent tank 111, solvent tank 112, and solvent tank 113, respectively.
- the analysis channel 106 and the analysis channel 107 are configured similarly to the analysis channel 105 . That is, the analysis channel 106 includes an analysis column 118, a liquid delivery device 119 that delivers a solvent to the analysis column 118 at high pressure, an injection valve 120 that is connected to the liquid delivery device 119 and introduces the sample into the analysis column 118, and a valve 121 for switching the solvent to be introduced into the liquid delivery device 119 .
- Valve 121 is connected to solvent tank 110, solvent tank 111, solvent tank 112, and solvent tank 113, respectively.
- the analysis channel 107 includes an analysis column 122, a liquid delivery device 123 that delivers a solvent to the analysis column 122 at high pressure, an injection valve 124 that is connected to the liquid delivery device 123 and introduces the sample into the analysis column 122, and a valve 125 for switching the solvent to be introduced into the liquid delivery device 1239 .
- Valve 125 is connected to solvent tank 110, solvent tank 111, solvent tank 112, and solvent tank 113, respectively.
- the pretreatment unit 101 introduces the sample into the separation unit 102 after pretreating the sample.
- the separation unit 102 introduces the sample pretreated by the pretreatment unit 101 into any one of the analysis channels 105, 106, and 107 via the valve 108 to perform separation processing.
- the sample subjected to separation processing is introduced into the detection section 103 via the valve 109 .
- the detection unit 103 detects an analyte component from the sample introduced from the separation unit 102 .
- FIG. 1 shows an analysis channel 105 connecting a valve 108 to a detection unit 103 to introduce a sample processed in a pretreatment unit 101 into each analysis channel 105, analysis channel 106, and analysis channel 107;
- a valve 109 is shown for switching between analysis channels 106 and 107 .
- the analysis channel 105, the analysis channel 106, and the analysis channel 107 each show one liquid transfer device and one solvent switching valve.
- any valve and liquid delivery device are not limited to one.
- the valve 108 may be provided with a nozzle in each analysis channel so that the sample can be introduced into each analysis channel without having a valve.
- FIG. 2 is a flowchart showing the analysis scheduling processing procedure for the entire automatic analyzer.
- the outline of the processing in each step of the flowchart shown in FIG. 2 is as follows.
- control unit 104 starts scheduling analysis.
- control unit 104 calculates an empty cycle in which the preprocessing for the analysis can be started next in the current analysis schedule.
- the control unit 104 determines whether or not the detection unit 103 can detect the sample when the analysis is started at the cycle calculated at step 202.
- the sample separation unit 102 has a plurality of analysis channels 105, 106, and 107, whereas the detection unit 103 has only one detector. It is necessary to schedule the timing of introducing the sample from 105 to 107 to the detector so as not to overlap.
- the process proceeds to step 205 . If it is determined in step 203 that detection of the sample by the detection unit 103 can be executed, the process proceeds to step 204 .
- step 204 the control unit 104 determines whether the sample separation process in the separation unit 102 can be executed when the analysis is started in the cycle calculated in step 202. If it is determined in step 204 that the sample separation process in the separation section 102 is not executable, the process proceeds to step 205 . If it is determined in step 204 that the sample separation process in the separation unit 102 can be executed, the process proceeds to step 206 .
- step 205 the control unit 104 delays the analysis start cycle by one cycle if it is determined that either step 203 or step 204 is unexecutable. The process then returns to step 203 .
- step 206 when the analysis is started in the cycle calculated in step 202, if both the sample separation unit 102 and the detection unit 103 can execute the analysis, the control unit 104 performs the analysis in the cycle. sign up.
- control unit 104 ends the above analysis scheduling.
- FIG. 3 is a flow chart showing the scheduling processing procedure for the sample separation unit 102, which corresponds to step 204 in the flow chart shown in FIG.
- the outline of the processing in each step is as follows.
- step 301 the control unit 104 starts scheduling the sample separation unit 102 for sample separation processing.
- control unit 104 determines whether or not there is an analysis channel capable of executing sample separation processing among the analysis channels 105-107. If the analysis channel does not exist, proceed to step 303 . Also, if there is an analysis channel, the process proceeds to step 304 .
- step 303 the control unit 104 determines that the sample separation process cannot be registered in the analysis schedule if there is no analysis channel capable of executing the sample separation process among the analysis channels 105-107. The process then proceeds to step 309 .
- step 304 the control unit 104 determines whether or not there are two or more analysis channels capable of executing sample separation processing among the analysis channels 105-107. If two or more analysis channels do not exist, proceed to step 305 . Also, if there are two or more analysis channels, the process proceeds to step 306 .
- step 305 if there is only one analysis channel capable of executing the sample separation process, the control unit 104 registers the sample separation process in the analysis schedule of that analysis channel. The process then proceeds to step 309 .
- step 306 when there are a plurality of analysis channels capable of executing sample separation processing, the control unit 104 determines whether or not there is an analysis channel that does not require solvent replacement among those analysis channels. . If there is no analysis channel that does not require solvent replacement, proceed to step 307 . If there is an analysis channel that does not require solvent replacement, proceed to step 308 .
- step 307 if there is no analysis channel that does not require solvent replacement, the control unit 104 performs sample separation processing according to the analysis schedule of the analysis channel that minimizes (shortest) processing cycles added for solvent replacement. to register. The process then proceeds to step 309 .
- step 308 if there is an analysis channel that does not require solvent replacement, the control unit 104 registers the sample separation process in the analysis schedule for that analysis channel. The process then proceeds to step 309 .
- control unit 104 terminates the scheduling of the separation unit 102 for sample separation processing.
- FIG. 4 is a diagram showing an example of scheduling for the separating unit 102.
- FIG. (a) of FIG. 4 is a diagram showing an overview of the analysis schedule of each of the analysis channels 105, 106, and 107 at the start of scheduling.
- the control unit 104 registers a separation process 404 using solvent A in the analysis schedule 401 for the analysis channel 105, and registers the separation process 404 in the analysis schedule 402 for the analysis channel 106.
- a separation process 405 using solvent B is registered, and a separation process 406 using solvent C is registered in the analysis schedule 403 for the analysis channel 107 .
- the next separation process 408 using the solvent C is scheduled. A processing procedure at this time will be described along each step of the flowchart in FIG.
- control unit 104 starts scheduling the separation unit 102 for the separation processing 408 . Then, go to step 302 .
- step 302 the control unit 104 is in a state in which the separation process can be executed in the three analysis channels 105, 106, and 107, so that there is an analysis channel capable of executing the separation process. Then, go to step 304.
- step 304 the control unit 104 is in a state in which the separation process can be executed in the three analysis channels 105, 106, and 107, so there are a plurality of analysis channels capable of executing the separation process. , and the process moves to step 306 .
- step 306 when registering the separation process 408 in the analysis schedule 401, since the solvents used in the separation process 404 and the separation process 408 performed immediately before in the analysis channel 105 are different, , inserting the solvent replacement process 409 before the separation process 408 is required.
- the separation process 408 is registered in the analysis schedule 402
- the separation process 405 performed immediately before in the analysis channel 106 and the solvent used in the separation process 408 are different.
- the insertion of a solvent replacement process 410 is required before the separation process 408 .
- the control unit 104 determines that there is an analysis channel that does not require solvent replacement, and proceeds to step 308 .
- control unit 104 registers the separation process 408 in the analysis schedule 403 of the analysis channel 107 that does not require solvent replacement. Then, go to step 309 .
- control unit 104 terminates the scheduling of the separation unit 102 for the separation processing 408 .
- the solvent C used in the next separation process 408 executed after the timing 407 and the solvents A, B and C used in the separation processes 404, 405 and 406 executed before the timing 407 are In comparison, by selecting the analysis channel 107 in which the separation process 406 using the solvent C has been performed and performing the separation process 408 after the timing 407, the solvent replacement process is not necessary and the solvent replacement process can be performed. can be avoided, and the decrease in throughput and solvent consumption can be suppressed.
- control method and automatic analyzer for an automatic analyzer having a liquid chromatograph equipped with a plurality of analysis channels in parallel a control method and an automatic analyzer for avoiding a decrease in throughput and an increase in solvent consumption due to solvent replacement are provided. can provide.
- Example 2 Next, Example 2 will be described.
- the second embodiment is an example of scheduling different from the first embodiment for the separation unit 102 in the same device configuration as the first embodiment.
- Example 2 The configuration of the automatic analyzer in Example 2 is the same as that of Example 1 shown in FIG. 1, so illustration and detailed description are omitted.
- the outline of the scheduling processing procedure for the entire automatic analyzer (FIG. 2) and the scheduling processing procedure for the separation unit 102 (FIG. 3) are the same as those in the first and second embodiments. Illustration is omitted.
- FIG. 5 is a diagram showing an example of scheduling for the separating unit 102.
- FIG. (a) of FIG. 5 is a diagram showing an overview of the analysis schedule of each of the analysis channels 105, 106, and 107 at the start of scheduling.
- the control unit 104 registers a separation process 504 using solvent A in the analysis schedule 501 for the analysis channel 105, and registers in the analysis schedule 502 for the analysis channel 106.
- a separation process 505 using solvent B is registered, and a separation process 506 using solvent C is registered in the analysis schedule 503 for the analysis channel 107 .
- the next separation process 508 using the solvent D is scheduled.
- the processing cycle required to execute solvent replacement from each of solvent A, solvent B, and solvent C to solvent D is By nature, let solvent A be defined as 2 cycles, solvent B as 2 cycles, and solvent C as 1 cycle. A processing procedure at this time will be described along each step of the flowchart in FIG.
- control unit 104 starts scheduling the separation unit 102 for the separation processing 508 .
- the process then moves to step 302 .
- step 302 the control unit 104 is in a state in which the separation process can be executed in the three analysis channels 105, 106, and 107, so that there is an analysis channel capable of executing the separation process. Then, go to step 304.
- step 304 the control unit 104 is in a state in which the separation process can be executed in the three analysis channels 105, 106, and 107, so there are a plurality of analysis channels capable of executing the separation process. , and the process moves to step 306 .
- control unit 104 determines that solvent replacement is necessary in any of the analysis channels 105 , 106 and 107 , and proceeds to step 307 .
- step 307 when the separation process 508 using solvent D is registered in the analysis schedule 501, the processing cycle added for solvent replacement is two cycles, so the solvent replacement process is performed as shown in FIG. 509 is added. Similarly, when the separation process 508 is registered in the analysis schedule 502, the process cycle added for solvent replacement is two cycles, so the solvent replacement process 510 is added as shown in FIG. 5(c). .
- control unit 104 determines to register the separation process 508 in the analysis schedule 503 of the analysis channel 107 that has the fewest process cycles added for solvent replacement, and registers it together with the solvent replacement process 511 . Processing then proceeds to step 309 .
- control unit 104 terminates the scheduling of the separation unit 102 for the separation processing 508 .
- the solvent D used in the separation process 508 executed after the timing 507 and the solvents A, B and C used in the separation processes 504, 505 and 506 executed before the timing 507 are compared.
- the analysis channel 107 in which the separation process 506 of the minimum cycle has been performed among the cycles required for solvent replacement with the solvent D, and the separation process 508 after the timing 507 is performed. Time can be minimized, reducing throughput loss and solvent consumption.
- Example 2 similarly to Example 1, the control method of an automatic analyzer having a liquid chromatograph equipped with a plurality of analysis channels in parallel and the automatic analyzer reduced throughput and solvent consumption due to solvent replacement. It is possible to provide a control method and an automatic analyzer that avoid an increase in
- Example 3 shows an example of scheduling different from Example 1 and Example 2 for the separation unit 102 in the same device configuration as Example 1.
- FIG. 3 shows an example of scheduling different from Example 1 and Example 2 for the separation unit 102 in the same device configuration as Example 1.
- Example 3 The configuration of the automatic analyzer in Example 3 is the same as that of Example 1 shown in FIG. 1, so illustration and detailed description are omitted. Also, since the outline of the processing procedure (FIG. 2) of the entire scheduling of the automatic analyzer is the same as that of the first embodiment, the illustration is omitted.
- Example 3 the first scheduling method that prioritizes no solvent replacement or the shortest additional processing cycle due to solvent replacement, and the throughput is prioritized over the shortest additional processing cycle due to solvent replacement. and a second scheduling method to be used. That is, the analysis schedule has a first scheduling scheme and a second scheduling scheme. The user can use the input/output device 126 to select either the first scheduling method or the second scheduling method and instruct the control unit 104 .
- FIG. 6 is a flowchart when the scheduling method can be selected from the first scheduling method and the second scheduling method.
- the flowchart shown in FIG. 6 corresponds to step 204 in the flowchart shown in FIG.
- FIG. 7 is an example of scheduling for the separating unit 102.
- FIG. (a) of FIG. 7 is a diagram showing an overview of the analysis schedule of each of the analysis channels 105, 106, and 107 at the start of scheduling.
- a separation process 704 using solvent A is registered in the analysis schedule 701 for the analysis channel 105
- a separation process 705 using solvent B is registered in the analysis schedule 702 for the analysis channel 106.
- a separation process 706 using solvent C is registered in the analysis schedule 703 for the analysis channel 107 .
- the next separation processing 708 using the solvent C is scheduled.
- the processing cycle required to replace solvent A and solvent B with solvent C is defined as one cycle due to the properties of the solvents. shall be
- step 601 the control unit 104 starts scheduling the separation unit for the separation process 708 . Processing moves to step 602 .
- step 602 the control unit 104 determines whether or not there is an analysis channel capable of executing separation processing among the three analysis channels 105, 106, and 107. If there is no analysis flow path in which separation processing can be executed, the process proceeds to step 603 . Also, if there is an analysis flow path that can be subjected to separation processing, the process proceeds to step 604 . In the example shown in FIG. 7, the three analysis channels 105, 106, and 107 are in a state where the separation process can be executed, so the process moves to step 604.
- FIG. 7 the three analysis channels 105, 106, and 107 are in a state where the separation process can be executed, so the process moves to step 604.
- step 603 the control unit 104 determines that the sample separation process cannot be registered in the analysis schedule if there is no analysis channel capable of executing the sample separation process among the analysis channels 105-107. The process then proceeds to step 611 .
- step 604 the control unit 104 determines whether or not there are two or more analysis channels capable of executing the separation process among the three analysis channels 105, 106, and 107. judge. If it is determined that there are not two or more analysis channels in which separation processing can be performed, the process proceeds to step 605 . If it is determined that there are two or more analysis channels in which separation processing can be performed, the process proceeds to step 606 . In the example shown in FIG. 7, there are two or more analysis flow paths capable of executing separation processing, so the process moves to step 606 .
- step 605 if there is only one analysis channel capable of executing the sample separation process, the control unit 104 registers the sample separation process in the analysis schedule of that analysis channel. Processing then proceeds to step 611 .
- control unit 104 determines whether or not the first scheduling method is being selected. If the first scheduling method has not been selected, go to step 607 . If it is determined that the first scheduling method is being selected, the process moves to step 608 .
- step 608 the process moves to step 608 because the first scheduling method is being selected.
- step 607 the control unit 104 registers the analysis schedule of the analysis channel whose analysis is completed earliest, including solvent replacement, in the analysis schedule. Processing then proceeds to step 611 .
- control unit 104 determines whether or not there is an analysis channel that does not require solvent replacement. If it is determined that there is no analysis channel that does not require solvent replacement, the process proceeds to step 609 . If it is determined that there is an analysis channel that does not require solvent replacement, the process proceeds to step 610 .
- the control unit 104 determines that there is an analysis channel that does not require solvent replacement, and proceeds to step 610 .
- step 609 if there is no analysis channel that does not require solvent replacement, the control unit 104 performs sample separation processing according to the analysis schedule of the analysis channel that minimizes (shortest) processing cycles added for solvent replacement. to register. The process then proceeds to step 611 .
- control unit 104 registers the separation process 708 in the analysis schedule 703 of the analysis channel 107 that does not require solvent replacement. The process then moves to step 611 .
- control unit 104 terminates the scheduling of the separation unit 102 for the separation process 708 .
- step 606 the second scheduling method is selected instead of the first scheduling, so the process moves to step 607.
- separation processing 708 is scheduled to be completed at timing 711 .
- the separation process 708 is scheduled to be completed at the timing 712 after the timing 711 .
- the separation process 708 is registered in the analysis schedule 703
- the solvent used in the separation process 706 performed immediately before in the analysis channel 107 is the same as the solvent used in the separation process 708, so (d ), the separation process 708 is scheduled to be completed at timing 712 because there is no need to perform a solvent replacement process.
- the timing 712 is the timing after the timing 711. Therefore, the control unit 104 determines to register the separation process 708 in the analysis schedule 70 1 of the analysis channel 105 scheduled to complete the separation process 708 earliest, and registers it together with the solvent replacement process 709 . The process then moves to step 611 .
- control unit 104 terminates the scheduling of the separation unit for the separation process 708 .
- the first scheduling method it is possible to minimize the amount of solvent consumption by minimizing the additional processing due to solvent replacement.
- the second scheduling method the analysis can be completed more quickly, and the throughput of the automatic analyzer can be improved.
- the analysis schedule is such that the analysis flow path used for the next separation process 708 among the plurality of analysis flow paths 105 to 107 does not require the solvent replacement process.
- the first scheduling method for determining the flow path and the analysis flow path to be used for the next separation process 708 among the plurality of analysis flow paths 105 to 107 is the analysis flow path 107 determined not to require the solvent replacement process.
- separation processing completion timing 712, and separation processing completion timings 711 and 712 of analysis flow channels 709 and 710 determined to require solvent replacement processing. and a second scheduling scheme determined as 105 .
- the automatic analyzer has an input/output device 126, and either the first scheduling method or the second scheduling method is selected by the input device 126.
- Example 3 similarly to Examples 1 and 2, in the control method and the automatic analyzer of an automatic analyzer having a liquid chromatograph having a plurality of analysis channels in parallel, the decrease in throughput due to solvent replacement And it is possible to provide a control method and an automatic analyzer that avoid an increase in solvent consumption.
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Abstract
Description
図1は、本発明が適用される自動分析装置の構成を示す概略図である。
つまり、分析流路106は、分析カラム118と、分析カラム118へ溶媒を高圧送液する送液装置119と、送液装置119に接続され、試料を分析カラム118へ導入する注入バルブ120と、送液装置119へ導入する溶媒を切り替えるバルブ121とを有する。バルブ121は溶媒タンク110と、溶媒タンク111と、溶媒タンク112と、溶媒タンク113とのそれぞれに接続されている。
次に、実施例2について説明する。
次に、実施例3について説明する。実施例3は、実施例1と同様の装置構成において、分離部102に対して、実施例1及び実施例2とは異なるスケジューリングの例を示すものである。
Claims (8)
- 試料の前処理を行う前処理部と、互いに並列に配置された複数の分析流路を有する液体クロマトグラフにより、前記前処理部で処理された試料を、複数種類の溶媒を使用して分離する分離処理を行う分離部と、前記分離部で分離された試料を検出する検出部と、前記前処理部、前記分離部及び前記検出部を制御する制御部と、を備え、前記制御部にて分析実行前に分析スケジュールを作成し、作成された前記分析スケジュールに基づいて前記試料を分析する自動分析装置の制御方法において、
互いに種類が異なる溶媒を用いた複数の分離処理を、前記複数の分析流路のうちのいずれを使用して、互いに時間差を設けて導入するかを決定し、
前記互いに時間差を設けて導入するかが決定された前記複数の分離処理の開始後のタイミングに行われる、次の分離処理に用いられる溶媒と、前記互いに時間差を設けて導入するかが決定された前記複数の分離処理に用いられるそれぞれの前記溶媒とを比較し、
前記複数の分離処理に使用された前記分析流路に対して溶媒置換処理が必要か否かを判定し、
前記溶媒置換処理が必要か否かの前記判定に従って、前記複数の分析流路のうちの前記次の分離処理に使用する分析流路を決定し、前記分析スケジュールを作成する、
ことを特徴とする自動分析装置の制御方法。 - 請求項1に記載の自動分析装置の制御方法において、
前記複数の分析流路のうちの前記次の分離処理に使用する分析流路は、前記溶媒置換処理が必要ではないと判定した分析流路であることを特徴とする自動分析装置の制御方法。 - 請求項1に記載の自動分析装置の制御方法において、
前記複数の分離処理に使用された前記分析流路に対して溶媒置換処理が必要か否かを判定し、前記分析流路のすべてに対して前記溶媒置換処理が必要と判定したときは、前記複数の分析流路のうち前記溶媒置換処理に必要なサイクルが最小である分析流路を前記次の分離処理に使用する分析流路と決定することを特徴とする自動分析装置の制御方法。 - 請求項1に記載の自動分析装置の制御方法において、
前記分析スケジュールは、
前記複数の分析流路のうちの前記次の分離処理に使用する分析流路は、前記溶媒置換処理が必要ではないと判定した分析流路と決定する第一のスケジューリング方式と、
前記複数の分析流路のうちの前記次の分離処理に使用する分析流路は、
前記溶媒置換処理が必要でないと判定された分析流路の分離処理完了タイミングと、前記溶媒置換処理が必要であると判定された分析流路の分離処理完了タイミングと、のうちの最も早く分離処理を完了すると判定した分析流路と決定する第二のスケジューリング方式と、
を有し、
前記自動分析装置は、入出力装置を備え、前記入出力装置により前記第一のスケジューリング方式と、前記第二のスケジューリング方式と、のうちのいずれかが選択されることを特徴とする自動分析装置の制御方法。 - 試料の前処理を行う前処理部と、互いに並列に配置された複数の分析流路を有する液体クロマトグラフにより、前記前処理部で処理された試料を、複数種類の溶媒を使用して分離する分離処理を行う分離部と、前記分離部で分離された試料を検出する検出部と、前記前処理部、前記分離部及び前記検出部を制御する制御部と、を備え、前記制御部にて分析実行前に分析スケジュールを作成し、作成された前記分析スケジュールに基づいて前記試料を分析する自動分析装置において、
前記制御部は、互いに種類が異なる溶媒を用いた複数の分離処理を、前記複数の分析流路のうちのいずれを使用して、互いに時間差を設けて導入するかを決定し、
前記互いに時間差を設けて導入するかが決定された前記複数の分離処理の開始後のタイミングに行われる、次の分離処理に用いられる溶媒と、前記互いに時間差を設けて導入するかが決定された前記複数の分離処理に用いられるそれぞれの前記溶媒とを比較し、
前記複数の分離処理に使用された前記分析流路に対して溶媒置換処理が必要か否かを判定し、
前記溶媒置換処理が必要か否かの前記判定に従って、前記複数の分析流路のうちの前記次の分離処理に使用する分析流路を決定し、前記分析スケジュールを作成する、
ことを特徴とする自動分析装置。 - 請求項5に記載の自動分析装置において、
前記制御部は、前記複数の分析流路のうちの前記次の分離処理に使用する分析流路を、前記溶媒置換処理が必要ではないと判定した分析流路と決定することを特徴とする自動分析装置。 - 請求項5に記載の自動分析装置において、
前記制御部は、
前記複数の分離処理に使用された前記分析流路に対して溶媒置換処理が必要か否かを判定し、前記分析流路のすべてに対して前記溶媒置換処理が必要と判定したときは、前記複数の分析流路のうち前記溶媒置換処理に必要なサイクルが最小である分析流路を前記次の分離処理に使用する分析流路と決定することを特徴とする自動分析装置。 - 請求項5に記載の自動分析装置において、
前記分析スケジュールは、
前記複数の分析流路のうちの前記次の分離処理に使用する分析流路は、前記溶媒置換処理が必要ではないと判定した分析流路と決定する第一のスケジューリング方式と、
前記複数の分析流路のうちの前記次の分離処理に使用する分析流路は、
前記溶媒置換処理が必要でないと判定された分析流路の分離処理完了タイミングと、前記溶媒置換処理が必要であると判定された分析流路の分離処理完了タイミングと、のうちの最も早く分離処理を完了すると判定した分析流路と決定する第二のスケジューリング方式と、
を有し、
前記自動分析装置は、入出力装置を備え、前記制御部は、前記入出力装置により前記第一のスケジューリング方式と、前記第二のスケジューリング方式と、のうちのいずれか選択されたスケジューリング方式に従って、前記分析スケジュールを作成することを特徴とする自動分析装置。
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JPS6332367A (ja) * | 1986-07-25 | 1988-02-12 | Japan Spectroscopic Co | 前処理による待ち時間をなくしたオ−トサンプラ |
JP2006258732A (ja) * | 2005-03-18 | 2006-09-28 | Shimadzu Corp | 液体クロマトグラフ分析装置 |
US20130014566A1 (en) * | 2011-07-15 | 2013-01-17 | Marks Aaron N | Method for Automatic Optimization Of Liquid Chromatography Autosampler |
WO2014068786A1 (ja) | 2012-11-05 | 2014-05-08 | 株式会社島津製作所 | クロマトグラフ分析装置 |
WO2017216934A1 (ja) * | 2016-06-16 | 2017-12-21 | 株式会社日立ハイテクノロジーズ | クロマトグラフ質量分析装置、及び制御方法 |
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