WO2022011346A1 - System and method of treating food products - Google Patents

System and method of treating food products Download PDF

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Publication number
WO2022011346A1
WO2022011346A1 PCT/US2021/041291 US2021041291W WO2022011346A1 WO 2022011346 A1 WO2022011346 A1 WO 2022011346A1 US 2021041291 W US2021041291 W US 2021041291W WO 2022011346 A1 WO2022011346 A1 WO 2022011346A1
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WIPO (PCT)
Prior art keywords
ozone
heating
food product
duration
treatment
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PCT/US2021/041291
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French (fr)
Inventor
Zhongli Pan
Ragab KHIR
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University of California Berkeley
University of California San Diego UCSD
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University of California Berkeley
University of California San Diego UCSD
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Publication of WO2022011346A1 publication Critical patent/WO2022011346A1/en
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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/70Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/704Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/05Preservation of foods or foodstuffs, in general by heating using irradiation or electric treatment
    • A23B2/055Preservation of foods or foodstuffs, in general by heating using irradiation or electric treatment with infrared rays
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/90Preservation of foods or foodstuffs, in general by drying or kilning; Subsequent reconstitution
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B7/00Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
    • A23B7/005Preserving by heating
    • A23B7/01Preserving by heating by irradiation or electric treatment
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B7/00Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
    • A23B7/02Dehydrating; Subsequent reconstitution
    • A23B7/0215Post-treatment of dried fruits or vegetables
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B7/00Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
    • A23B7/144Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor

Definitions

  • the present invention is directed generally to processing food products, and in particular to systems and methods of sanitizing and drying food products.
  • SO2 sulfur dioxide
  • SO2 is widely used in fruit drying process for maintaining light product color and ensuring microbial safety.
  • SO2 may cause health problems in humans and may further result in environmental damage if not disposed of properly. Therefore, it would be desirable to identify an alternative to the utilization of SO2.
  • Figure 1 is a block diagram of a sequential ozone treatment and Infrared (IR) heating process according to some embodiments.
  • Figure 2 is a block diagram of an ozone treatment apparatus according to some embodiments.
  • FIG. 3 is a block diagram of an infrared (IR) heating apparatus according to some embodiments.
  • Figures 4A and 4B are graphs illustrating the effect of ozone treatment for various time periods and concentration levels on microorganism levels according to some embodiments.
  • Figure 5 is a photograph illustrating the color of dried grapes produced using ozone treatment according to some embodiments.
  • Figure 6 is a graph illustrating surface temperature and internal temperature of a food product heated using IR heating methods according to some embodiments.
  • Figures 7A and 7B are graphs illustrating the effect of IR heating on microorganism levels according to some embodiments.
  • Figure 8 is a photograph illustrating the color of dried grapes produced using IR heating for various durations of time according to some embodiments.
  • Figure 9 is a chart illustrating the effect of IR heating on PPO activity according to some embodiments.
  • Figures 10A and 10B are graphs illustrating the effect of sequential use of ozone treatment and IR heating on microorganism levels according to some embodiments.
  • Figure 11 is a photograph illustrating the color of dried grapes produced using sequential application of ozone treatment and IR heating according to some embodiments.
  • the present invention is directed generally to the treatment of food products.
  • the treatment process utilizes the sequential application of ozone gas to the food product followed by infrared (IR) heating of the food product.
  • IR infrared
  • the combination of ozone treatment and IR heating acts to sanitize the food products (i.e., reduce the microbial load) while inactivating browning enzymes that cause the browning of food products.
  • the utilization of IR heating as a pre treatment method to hot air drying results in a reduction of hot air drying time. In some embodiments, the reduction in hot air drying time is 30% or more.
  • the sequential use of ozone treatment and IR heating provides a synergetic effect on the reduction of microbial load and product color change during drying process.
  • sequential ozone treatment and IR heating provides functionality commensurate with that of traditional Sulfur dioxide (SO2) treatment without the corresponding health and environmental concerns associated with SO2.
  • FIG. 1 is a block diagram of a sequential ozone treatment and Infrared (IR) heating process 100 according to some embodiments.
  • grapes 102 are utilizes as an exemplary food product to be dried.
  • other types of fruits and or vegetables may be utilized (e.g., apples, bananas, beans, etc.).
  • food products may include other food products, such as meats (e.g., fish, chicken).
  • O3 treatment relies on the exposure of the grapes (or other food product) to ozone (O3).
  • the ozone in a gaseous state is applied to the grape 102.
  • the concentration of the gaseous ozone applied to the food product may be varied according to the application.
  • the ozone concentration is less than 200 parts-per- million (ppm).
  • the ozone concentration may be between 200-400 ppm, between 400-600 ppm, between 600-800 ppm, or greater than 800 ppm.
  • the exposure time of the zone treatment may be varied according to the application.
  • the exposure time of the food product to the ozone treatment is approximately 30 minutes.
  • the exposure time is between 30 minutes and 60 minutes.
  • the exposure time is between 60 minutes and 90 minutes.
  • the exposure time is between 90 minutes and 120 minutes.
  • the exposure time is greater than 120 minutes.
  • ozone treatment acts to reduce the microbial load of the food product.
  • the reduction in microbial load caused by the ozone treatment may be a result of oxidation action initiated by the ozone treatment with respect to the glycoproteins and glycolipids of the cell membrane which causes changes to the permeability of the cell, resulting in the leakage of cellular material.
  • ozone treatment causes the oxidizing of polyunsaturated fatty acid to acid peroxides, which modifies the cellular activities of the treated microbial.
  • ozone treatment may also act on some sensitive components, such as thymine, cytosine and uracil, causing a decrease in the transcription activity of DNA. The net result though, is an overall reduction in microbial load associated with the food product.
  • the ozone treatment may cause an oxidation of cellular components of amino acids in enzymes. In some embodiments, this results in a reduction of activity of browning enzymes and therefore may result in less color change of the food product.
  • the color lightness of the grapes may be maintained via ozone treatment of the grapes.
  • the ozone treated food product e.g., grapes, in the embodiment shown in Figure 1 are heated using infrared (IR) heating.
  • Heating times and temperatures may be varied according to the application. For example, in some embodiments heating times may be between approximately one and five minutes. In other embodiments, heating times may be increased as necessary.
  • heating intensity may be varied according to application.
  • the IR emitter (shown in more detail in Figure 4) is located at a known distance (e.g., 15 cm) from the food product and operates at a known radiation intensity (e.g., 4685 W/m 2 ). In other embodiments, the IR radiation intensity may be higher or lower - for example, between 3,500-6,000 W/m 2 .
  • IR heating is controlled to heat the food product to a desired temperature.
  • the heating is controlled based on monitored surface temperatures of the food product (e.g., grapes).
  • the duration and intensity of IR heating is selected to increase the surface temperature of the food product to a desired temperature.
  • the IR heating process ends when the threshold temperature is reached.
  • the IR heating process maintains the food product at the desired temperature for a duration of time.
  • the surface temperature is heated to a temperature equal to or greater than 60° Celsius (C); in some embodiments equal to or greater than 70°C; in some embodiments equal to or greater than 80°C and in some embodiments equal to or greater than 90°C.
  • the duration and intensity of IR heating is selected to increase the interior temperature of the food product to threshold temperature. Once again, in some embodiments IR heating continues until the threshold internal temperature is reach, while in other embodiments the IR heating continues for a duration of time after the internal threshold temperature is reach.
  • the threshold internal temperature is greater than or equal to 50°C; in some embodiments the internal temperature is greater than or equal to 60°C, in some embodiments the internal temperature is greater than or equal to 70°C, and in some embodiments the internal temperature is greater than or equal to 80°C.
  • IR heating at step 106 causes a reduction in microbial load of the food product.
  • IR heating acts to thermally inactivate the microorganisms by damaging DNA, RNA, ribosome, cytoplasm, cell envelope, and protein in the microorganisms located on the food product (e.g., grapes).
  • IR heating causes an inactivation of browning enzymes
  • processing at step 108 includes additional drying of the food product utilizing a hot air drying method to dehydrate the food product.
  • processing at step 108 includes additional drying of the food product utilizing a hot air drying method to dehydrate the food product.
  • the grapes 102 are dehydrated to produce raisins.
  • the sequential application of ozone treatment followed by IR heating provides several unexpected benefits. As discussed in more detail with respect to the results of experiments conducted, the sequential application of ozone treatment and IR heating provides a synergistic effect on both reduction of microbial loads and deactivation of browning enzymes responsible for browning of certain food products (e.g., grapes, apples, fish, etc.).
  • the reduction in microbial load utilizing the sequential application of ozone treatment and IR heating is greater than the reduction achieved with either ozone-only treatment and IR heating-only treatment.
  • the sequential application of ozone treatment and IR heating provided a 4- 5 log reduction of microbial load, which is greater than the microbial load reduction achievable via ozone-only treatment or IR-only heating.
  • the sequential application of ozone treatment and IR heating reduced browning of the food product as compared with food product that received ozone-only treatment and food product that received IR heating-only treatment prior to drying.
  • the sequential application of ozone-treatment following by IR heating reduced the drying time required for the food product. In some embodiments, the drying time (utilizing a hot air drying method described with respect to step 108) was reduced by over 33% when utilizing the sequential application of ozone treatment and IR heating of the food products.
  • FIG. 2 is a block diagram of an ozone treatment apparatus 200 according to some embodiments.
  • ozone treatment apparatus 200 includes an air cylinder 202, ozone generator 204, ozone chamber 206, and ozone analyzer 208.
  • ambient air may be provided to ozone generator 204.
  • dry air i.e., low-humidity
  • ozone generator 204 generates ozone (O3) that is provided in a gaseous state to ozone chamber 206.
  • ozone analyzer 208 and associated sensor 210 is utilized to monitor the concentration of ozone (O3) within the ozone chamber 206 and provides feedback to ozone generator 204 to either increase or decrease the flow of ozone into the ozone chamber 206.
  • the food product is placed within the ozone chamber 206 on a tray.
  • the food product is arranged in a single layer that allows exposure of the food product to the ozone environment. As described in more detail below, the ozone concentration and duration of exposure may be modified depending on the application.
  • temperature measuring device 306 monitors the surface temperature of the food product or sample being heated. In some embodiments, temperatures measuring device 306 monitors the internal temperature of the food product or sample being heated. In some embodiments, temperature measuring device 306 may monitor a combination of chamber temperature, surface temperature, and/or internal temperature of the food product. Feedback from the temperature measuring device 306 is provided to data acquisition device 308, which may be utilized to control (i.e., increase or decrease) the IR irradiation. For example, in the embodiment shown in Figure 3, data acquisition 308 controls a gas valve 304 to increase or decrease the flow of gas (e.g., natural gas) to the IR heating elements.
  • gas e.g., natural gas
  • Figure 4a is a graph illustrating microorganism population as a result of various concentrations of ozone treatment and various durations of ozone treatment according to some embodiments; and Figure 4b is a graph illustrating the reduction in microorganism population as a result of various concentrations of ozone treatment and various durations of ozone treatment according to some embodiments.
  • Figure 5 illustrates the effect of ozone concentration on the color change of grapes according to some embodiments. The results illustrated in Figures 4a-4b and 5 were obtained by first inoculating grape samples with E. faecium NRRL-B2354 to initial concentration of 10 7 CFU/g to provide the samples with a known microbial load.
  • ozone concentrations ranging from 200 ppm to 800 ppm and treatment durations ranging from 30 min to 120 min were tested to determine microorganism reduction and color change of grape.
  • the samples were spread as single layer in a stainless- steel basket and treated with ozone under aforementioned concentrations and treatment times.
  • the effect of each treatment condition on the microbial load reduction was evaluated right after each ozone treatment.
  • the treated grapes were dried using hot air at 60°C to a finial moisture content of 10+1% (wet basis). Then the color indices (L, a, b) of dried grapes was measured using a color meter.
  • the populations of E E.
  • faecium significantly decreased with the increase of ozone concentration and exposure time.
  • the ozone treatments at 200, 400, 600, and 800 ppm for 120 min achieved 1.96, 2.85, 3.07, and 3.60 log reductions of E. faecium, respectively ( Figure 4b). This means that the ozone treatment is effective in reducing microbial loads, but the maximum reduction was less than 4 log.
  • color testing indicates that ozone treatment acts to at least partially reduce the browning enzyme activity of the samples tested as illustrated visually in Figure 5 and quantitatively in table 1, shown below.
  • ozone treatment resulted in an increase to the L* value and a decrease in the a* value.
  • the L* value increased from 28.78+1.64 to 31.01+1.1
  • the a* value decreased from 9.41+1.08 to 8.73+2.00 (Table 1).
  • FIG. 5 is a graph illustrating temperature profile of grapes during infrared heating according to some embodiments.
  • the grape samples were spread as a single layer on a stainless- steel wire mesh placed at distance of 15 cm from the IR emitter and treated under radiation intensity of 4685 W/m 2 for 1 , 2, 3, 4, and 5 minutes, respectively.
  • Measured surface temperature is illustrated by line 600 and internal temperature is illustrated by line 602.
  • no ozone treatment was applied to the grapes prior to IR treatment.
  • the center and surface temperatures, color change, and polyphenol oxidase (PPO) enzyme activity were determined.
  • the samples were dried using a hot air dryer at 60°C.
  • the IR heating produced very light color dried product (p ⁇ 0.05) ( Figure 8).
  • the F* value of dried grapes was significantly (p ⁇ 0.05) higher than that of the untreated grapes.
  • the F* value of untreated grapes was 27.7+3.6 (Table 2).
  • the a* values of dried grapes were much lower than those of the untreated samples.
  • the a* value of treated grapes was 2.9+0.5, while the a* value of untreated grapes was 7.9+2.8 (Table 2).
  • Figures 10a, 10b, 11, and 12 illustrate the results of experiments that utilized sequential treatments of food products utilizing ozone treatment and IR heating. Experimental results of the sequential utilization of ozone treatment and IR heating with the use of ozone treatment only and IR heating only.
  • the food product e.g., grapes
  • ozone under different gaseous ozone concentrations ranging from 200 to 800 ppm for durations of time ranging from 60 and 120 min, respectively.
  • the samples were heated using infrared (IR) for 5 min.
  • IR infrared
  • the samples were dried using hot air drying at 60 °C to a final moisture content of 10+1.0% (w.b). The drying time was recorded and compared with that of untreated samples.
  • the microbial load was evaluated right after sequential ozone and IR heating treatment. The color of the dried grapes was measured after hot air drying.
  • the embodiments of the present technology of using sequential ozone and IR heating treatment as a pre-treatment method of hot air drying provide the following alternative variants to the previous arts.
  • Ozone treatment has high antimicrobial capacity and can effectively destroy microorganisms and slightly improve the color of treated product by reducing the activities of enzymes causing browning.
  • IR has high heating rate and powerful thermal inactivation capability of microorganisms and the enzymes causing browning and significantly enhances the lightness of the treated product and reduces the drying time for the hot air drying.
  • Separate ozone treatment or IR heating alone cannot achieve 4 to 5 log reduction of microbial load and cannot produce the dried products with very light color.
  • the sequential ozone and IR heating treatment as a pre-treatment method of hot air had positive synergetic effect on reduction of microbial load and product color change during drying process. It can achieve more than 5 log reduction of microbial load that ensured food safety, and produce the dried products with very light color, the new processing method also can reduce the drying time of hot air drying. Sequential ozone treatment and IR heating can achieve similar functions of Sulfur dioxide (SO2) treatment in fruit drying process and produce dried fruits with ensured safety and light color without any health and environmental concerns.
  • SO2 sulfur dioxide
  • a method of treating food products includes applying an ozone treatment to the food product, wherein the ozone treatment includes exposing the food product to a gaseous ozone environment.
  • the method further includes applying infrared (IR) heating to the ozone treated food product.
  • IR infrared
  • the method of the preceding paragraph ca optionally include, additionally and/or alternatively any, one or more of the following features, configurations and/or additional components
  • the method may include drying the food product following infrared heating utilizing a hot air dryer, wherein IR heating of the ozone treated food product reduces the drying time of the food product.
  • the method may further include wherein the gaseous ozone environment has an ozone concentration greater than 200 parts per million (ppm).
  • the method may further include wherein the gaseous ozone environment has an ozone concentration greater than 400 ppm.
  • the method may further include wherein the gaseous ozone environment has an ozone concentration greater than 600 ppm.
  • the method may further include wherein the gaseous ozone environment has an ozone concentration greater than 800 ppm.
  • the method may further include wherein the ozone treatment is applied for a duration of at least 30 minutes.
  • the method may further include wherein the ozone treatment is applied for a duration of at least 60 minutes.
  • the method may further include wherein the ozone treatment is applied for a duration of at least 90 minutes.
  • the method may further include wherein the ozone treatment is applied for a duration of at least 120 minutes.
  • the method may further include wherein the IR heating is applied for a duration of at least one minute. [0043] The method may further include wherein the IR heating is applied for a duration of at least two minutes.
  • the method may further include wherein the IR heating is applied for a duration of at least three minutes.
  • the method may further include wherein the IR heating is applied for a duration of at least four minutes.
  • the method may further include wherein the IR heating is applied for a duration of at least five minutes.
  • the method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 50° Celsius (C).
  • the method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 60° Celsius (C).
  • the method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 70° Celsius (C).
  • a food processing apparatus may include an ozone treatment apparatus including an ozone chamber for exposing a food product to a gaseous ozone environment and an infrared (IR) heating apparatus including infrared heating elements for heating the ozone treated food product.
  • IR infrared
  • the food processing apparatus of the preceding paragraph can optionally include, additionally and/or alternatively any, one or more of the following features, configurations and/or additional components.
  • the apparatus may further include a hot air drying apparatus configured to dry the ozone treated and IR heated food product.
  • the ozone treatment apparatus may include an ozone analyzer that measures ozone concentrations in the ozone chamber.
  • the IR heating element includes a temperature monitoring device that measures one of IR heating apparatus air temperature, food product surface temperature, and/or interior temperature of the food product.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Storage Of Fruits Or Vegetables (AREA)
  • Freezing, Cooling And Drying Of Foods (AREA)

Abstract

A method of treating food products utilizes the sequential application of ozone treatment and infrared (IR) heating. The ozone treatment includes exposing the food product to a gaseous ozone environment for a duration of time. The IR heating includes applying IR irradiation to increase the temperature of the food product.

Description

SYSTEM AND METHOD OF TREATING FOOD PRODUCTS
CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Provisional Patent Application No. 63/050,472, filed July 10, 2020, which is incorporated by reference herein in its entirety.
BACKGROUND
[0002] The present invention is directed generally to processing food products, and in particular to systems and methods of sanitizing and drying food products.
[0003] A number of food products - in particular fruits and vegetables - utilize drying processes. Sulfur dioxide (SO2) is widely used in fruit drying process for maintaining light product color and ensuring microbial safety. However, if not properly handled SO2 may cause health problems in humans and may further result in environmental damage if not disposed of properly. Therefore, it would be desirable to identify an alternative to the utilization of SO2. In addition, it would be beneficial if the alternative provided microbial safety while maintaining the desired light color change associated with SO2 utilization.
DESCRIPTION OF THE DRAWINGS
[0001] Figure 1 is a block diagram of a sequential ozone treatment and Infrared (IR) heating process according to some embodiments.
[0002] Figure 2 is a block diagram of an ozone treatment apparatus according to some embodiments.
[0003] Figure 3 is a block diagram of an infrared (IR) heating apparatus according to some embodiments.
[0004] Figures 4A and 4B are graphs illustrating the effect of ozone treatment for various time periods and concentration levels on microorganism levels according to some embodiments.
[0005] Figure 5 is a photograph illustrating the color of dried grapes produced using ozone treatment according to some embodiments.
[0006] Figure 6 is a graph illustrating surface temperature and internal temperature of a food product heated using IR heating methods according to some embodiments. [0007] Figures 7A and 7B are graphs illustrating the effect of IR heating on microorganism levels according to some embodiments.
[0008] Figure 8 is a photograph illustrating the color of dried grapes produced using IR heating for various durations of time according to some embodiments.
[0009] Figure 9 is a chart illustrating the effect of IR heating on PPO activity according to some embodiments.
[0010] Figures 10A and 10B are graphs illustrating the effect of sequential use of ozone treatment and IR heating on microorganism levels according to some embodiments.
[0011] Figure 11 is a photograph illustrating the color of dried grapes produced using sequential application of ozone treatment and IR heating according to some embodiments.
DETAILED DESCIRPTION
[0012] The present invention is directed generally to the treatment of food products. The treatment process utilizes the sequential application of ozone gas to the food product followed by infrared (IR) heating of the food product. The combination of ozone treatment and IR heating acts to sanitize the food products (i.e., reduce the microbial load) while inactivating browning enzymes that cause the browning of food products. In addition, the utilization of IR heating as a pre treatment method to hot air drying results in a reduction of hot air drying time. In some embodiments, the reduction in hot air drying time is 30% or more. In particular, the sequential use of ozone treatment and IR heating provides a synergetic effect on the reduction of microbial load and product color change during drying process. In addition, it has been found that sequential ozone treatment and IR heating provides functionality commensurate with that of traditional Sulfur dioxide (SO2) treatment without the corresponding health and environmental concerns associated with SO2.
[0013] Figure 1 is a block diagram of a sequential ozone treatment and Infrared (IR) heating process 100 according to some embodiments. In the embodiment showing in Figure 1, grapes 102 are utilizes as an exemplary food product to be dried. In other embodiments, other types of fruits and or vegetables may be utilized (e.g., apples, bananas, beans, etc.). In some embodiments, food products may include other food products, such as meats (e.g., fish, chicken). At step 104, the grapes 102 receive ozone (O3) treatment. As described in more detail with respect to Figure 2, ozone treatment relies on the exposure of the grapes (or other food product) to ozone (O3). In some embodiments, the ozone in a gaseous state is applied to the grape 102. The concentration of the gaseous ozone applied to the food product may be varied according to the application. For example, in some embodiments the ozone concentration is less than 200 parts-per- million (ppm). In other embodiments, the ozone concentration may be between 200-400 ppm, between 400-600 ppm, between 600-800 ppm, or greater than 800 ppm. In some embodiments, the exposure time of the zone treatment may be varied according to the application. For example, in some embodiments the exposure time of the food product to the ozone treatment is approximately 30 minutes. In some embodiments the exposure time is between 30 minutes and 60 minutes. In some embodiments the exposure time is between 60 minutes and 90 minutes. In some embodiments the exposure time is between 90 minutes and 120 minutes. In some embodiments the exposure time is greater than 120 minutes.
[0014] As described in more detail below, ozone treatment acts to reduce the microbial load of the food product. In some embodiments, the reduction in microbial load caused by the ozone treatment may be a result of oxidation action initiated by the ozone treatment with respect to the glycoproteins and glycolipids of the cell membrane which causes changes to the permeability of the cell, resulting in the leakage of cellular material. In some embodiments, ozone treatment causes the oxidizing of polyunsaturated fatty acid to acid peroxides, which modifies the cellular activities of the treated microbial. In some embodiments, ozone treatment may also act on some sensitive components, such as thymine, cytosine and uracil, causing a decrease in the transcription activity of DNA. The net result though, is an overall reduction in microbial load associated with the food product.
[0015] In some embodiments, the ozone treatment may cause an oxidation of cellular components of amino acids in enzymes. In some embodiments, this results in a reduction of activity of browning enzymes and therefore may result in less color change of the food product. For example, in embodiments in which the food product being dried is grapes, the color lightness of the grapes may be maintained via ozone treatment of the grapes.
[0016] At step 106 the ozone treated food product (e.g., grapes, in the embodiment shown in Figure 1) are heated using infrared (IR) heating. Heating times and temperatures may be varied according to the application. For example, in some embodiments heating times may be between approximately one and five minutes. In other embodiments, heating times may be increased as necessary. In addition, heating intensity may be varied according to application. In some embodiments, the IR emitter (shown in more detail in Figure 4) is located at a known distance (e.g., 15 cm) from the food product and operates at a known radiation intensity (e.g., 4685 W/m2). In other embodiments, the IR radiation intensity may be higher or lower - for example, between 3,500-6,000 W/m2. Both the distance to the food product and the radiation intensity may be varied depending on the application. In some embodiments, IR heating is controlled to heat the food product to a desired temperature. In some embodiments the heating is controlled based on monitored surface temperatures of the food product (e.g., grapes). For example, in some embodiments the duration and intensity of IR heating is selected to increase the surface temperature of the food product to a desired temperature. In some embodiments the IR heating process ends when the threshold temperature is reached. In some embodiments, the IR heating process maintains the food product at the desired temperature for a duration of time. In some embodiments, the surface temperature is heated to a temperature equal to or greater than 60° Celsius (C); in some embodiments equal to or greater than 70°C; in some embodiments equal to or greater than 80°C and in some embodiments equal to or greater than 90°C. In some embodiments, the duration and intensity of IR heating is selected to increase the interior temperature of the food product to threshold temperature. Once again, in some embodiments IR heating continues until the threshold internal temperature is reach, while in other embodiments the IR heating continues for a duration of time after the internal threshold temperature is reach. In some embodiments, the threshold internal temperature is greater than or equal to 50°C; in some embodiments the internal temperature is greater than or equal to 60°C, in some embodiments the internal temperature is greater than or equal to 70°C, and in some embodiments the internal temperature is greater than or equal to 80°C.
[0017] As described in more detail below, IR heating at step 106 causes a reduction in microbial load of the food product. In some embodiments, IR heating acts to thermally inactivate the microorganisms by damaging DNA, RNA, ribosome, cytoplasm, cell envelope, and protein in the microorganisms located on the food product (e.g., grapes). In addition, IR heating causes an inactivation of browning enzymes
[0018] At step 108 the ozone-treated and IR-heated food product is further processed. For example, in the embodiment shown in Figure 1, processing at step 108 includes additional drying of the food product utilizing a hot air drying method to dehydrate the food product. For example, in the example illustrated the grapes 102 are dehydrated to produce raisins. The sequential application of ozone treatment followed by IR heating provides several unexpected benefits. As discussed in more detail with respect to the results of experiments conducted, the sequential application of ozone treatment and IR heating provides a synergistic effect on both reduction of microbial loads and deactivation of browning enzymes responsible for browning of certain food products (e.g., grapes, apples, fish, etc.). In particular, the reduction in microbial load utilizing the sequential application of ozone treatment and IR heating is greater than the reduction achieved with either ozone-only treatment and IR heating-only treatment. In some embodiments (described in more detail below), the sequential application of ozone treatment and IR heating provided a 4- 5 log reduction of microbial load, which is greater than the microbial load reduction achievable via ozone-only treatment or IR-only heating. Furthermore, the sequential application of ozone treatment and IR heating reduced browning of the food product as compared with food product that received ozone-only treatment and food product that received IR heating-only treatment prior to drying. Finally, the sequential application of ozone-treatment following by IR heating reduced the drying time required for the food product. In some embodiments, the drying time (utilizing a hot air drying method described with respect to step 108) was reduced by over 33% when utilizing the sequential application of ozone treatment and IR heating of the food products.
[0019] Figure 2 is a block diagram of an ozone treatment apparatus 200 according to some embodiments. In some embodiments, ozone treatment apparatus 200 includes an air cylinder 202, ozone generator 204, ozone chamber 206, and ozone analyzer 208. In some embodiments, ambient air may be provided to ozone generator 204. However, in the embodiment shown in Figure 2, dry air (i.e., low-humidity) stored by air cylinder 202 is provided to ozone generator 204. In response, ozone generator 204 generates ozone (O3) that is provided in a gaseous state to ozone chamber 206. In some embodiments, ozone analyzer 208 and associated sensor 210 is utilized to monitor the concentration of ozone (O3) within the ozone chamber 206 and provides feedback to ozone generator 204 to either increase or decrease the flow of ozone into the ozone chamber 206. In some embodiments, the food product is placed within the ozone chamber 206 on a tray. In some embodiments the food product is arranged in a single layer that allows exposure of the food product to the ozone environment. As described in more detail below, the ozone concentration and duration of exposure may be modified depending on the application.
[0020] Figure 3 is a block diagram of an infrared (IR) heating apparatus 300 according to some embodiments. In the embodiment shown in Figure 3, the IR heating apparatus 300 includes IR heating chamber 302. In some embodiments, IR heating chamber 302 is single-sided or double sided. The embodiment shown in Figure 3 illustrates double-sided heating, in which the food product (i.e., sample 303) is placed within the chamber 302 and is irradiated from both sides. In other embodiments, single-sided IR irradiation may be utilized. In some embodiments, a temperature measuring device 306 is utilized to monitor the temperature within the IR heating chamber 302. In some embodiments, temperature measuring device 306 monitors temperature within the IR heating chamber 302. In some embodiments, temperature measuring device 306 monitors the surface temperature of the food product or sample being heated. In some embodiments, temperatures measuring device 306 monitors the internal temperature of the food product or sample being heated. In some embodiments, temperature measuring device 306 may monitor a combination of chamber temperature, surface temperature, and/or internal temperature of the food product. Feedback from the temperature measuring device 306 is provided to data acquisition device 308, which may be utilized to control (i.e., increase or decrease) the IR irradiation. For example, in the embodiment shown in Figure 3, data acquisition 308 controls a gas valve 304 to increase or decrease the flow of gas (e.g., natural gas) to the IR heating elements. [0021] Figure 4a is a graph illustrating microorganism population as a result of various concentrations of ozone treatment and various durations of ozone treatment according to some embodiments; and Figure 4b is a graph illustrating the reduction in microorganism population as a result of various concentrations of ozone treatment and various durations of ozone treatment according to some embodiments. Figure 5 illustrates the effect of ozone concentration on the color change of grapes according to some embodiments. The results illustrated in Figures 4a-4b and 5 were obtained by first inoculating grape samples with E. faecium NRRL-B2354 to initial concentration of 107 CFU/g to provide the samples with a known microbial load.
[0022] As shown in Figures 4a and 4b, ozone concentrations ranging from 200 ppm to 800 ppm and treatment durations ranging from 30 min to 120 min were tested to determine microorganism reduction and color change of grape. The samples were spread as single layer in a stainless- steel basket and treated with ozone under aforementioned concentrations and treatment times. The effect of each treatment condition on the microbial load reduction was evaluated right after each ozone treatment. After ozone treatment, the treated grapes were dried using hot air at 60°C to a finial moisture content of 10+1% (wet basis). Then the color indices (L, a, b) of dried grapes was measured using a color meter. The populations of E. faecium significantly decreased with the increase of ozone concentration and exposure time. The ozone treatments at 200, 400, 600, and 800 ppm for 120 min achieved 1.96, 2.85, 3.07, and 3.60 log reductions of E. faecium, respectively (Figure 4b). This means that the ozone treatment is effective in reducing microbial loads, but the maximum reduction was less than 4 log.
[0023] In addition, color testing (shown in Figure 5) indicates that ozone treatment acts to at least partially reduce the browning enzyme activity of the samples tested as illustrated visually in Figure 5 and quantitatively in table 1, shown below. In general, ozone treatment resulted in an increase to the L* value and a decrease in the a* value. In particular, when the ozone concentration increased from 200 ppm to 800 ppm, and treatment time from 30 min to 120 min, the L* value increased from 28.78+1.64 to 31.01+1.1, and the a* value decreased from 9.41+1.08 to 8.73+2.00 (Table 1). This indicates that ozone treatment partially reduces the browning enzyme activity and slightly improves the color of dried grapes. In some embodiments, this may be a result of the ability of ozone to oxidize the cellular components of amino acids in enzymes, resulting in reduced activities.
Figure imgf000008_0001
TABLE 1
[0024] Figure 5 is a graph illustrating temperature profile of grapes during infrared heating according to some embodiments. In particular, in this experiment, the grape samples were spread as a single layer on a stainless- steel wire mesh placed at distance of 15 cm from the IR emitter and treated under radiation intensity of 4685 W/m2 for 1 , 2, 3, 4, and 5 minutes, respectively. Measured surface temperature is illustrated by line 600 and internal temperature is illustrated by line 602. In this experiment, no ozone treatment was applied to the grapes prior to IR treatment. The center and surface temperatures, color change, and polyphenol oxidase (PPO) enzyme activity were determined. After IR treatment, the samples were dried using a hot air dryer at 60°C. The effect of each IR heating treatment on the microbial load reduction was evaluated after the treatment. The color of IR treated grapes were measured after hot air drying. The obtained results revealed the IR heating increased the temperate of grape in a short time. It took only about 1.3 minute to heat the grape surface to temperature of 90 °C (as shown by line 600), while the center temperature (line 602) reached to 60°C and 70°C after 3.3 and 4.6 min of IR heating. The IR heating for 1, 2, 3, 4, and 5 min reduced the E. faecium population size by 0.26, 0.37, 0.70, 1.02, and 1.58 log CFU/g, respectively (Figure 7B), which were less than 5 log as normally required. As described above, in some embodiments the IR heating thermally inactivated the microorganisms by damaging DNA, RNA, ribosome, cytoplasm, cell envelope, and protein in the treated microorganism.
[0025] In addition, the IR heating produced very light color dried product (p<0.05) (Figure 8). After IR heating for 5 min, the F* value of dried grapes was significantly (p<0.05) higher than that of the untreated grapes. By increasing the IR heating time from 1 to 5 min, the F* value increased from 29.2+2.2 to 37.3+1.9, while the F* value of untreated grapes was 27.7+3.6 (Table 2). The a* values of dried grapes were much lower than those of the untreated samples. After 5 min of IR heating, the a* value of treated grapes was 2.9+0.5, while the a* value of untreated grapes was 7.9+2.8 (Table 2). This might be attributed to the effective thermal inactivation of browning enzymes (polyphenol oxidase (PPO) and peroxidase (POD)) caused by IR heating, which decreased the darkening of grapes. After IR heating for 5 min, the PPO residual activity in the grapes was lower than 10%. This means that a significant thermal inactivation of PPO occurred and most of the PPO activity was destroyed when the center temperature reached to above 60 °C (Figure 9).
Blanching time Parameters
(min) F* a* b*
0 27.7+3.6b 7.9+2.8ab 13.8+4.5a
1 29.2+2.2b 9.5+0.5a 14.5+2. la 2 28.2±4.2b 6.9+1.9ab 14.2+1.4a
3 29.7+3. lb 6.5+1.0ab 16.3+2.9a
4 33.7+2.4ab 5.2+l.Obc 18.1+2.2a
5 37.3+1.9a 2.9+0.5c 17.3+2.3a
TABLE 2
[0026] The obtained results clearly revealed that IR heating resulted in a powerful inactivation of microorganisms and the browning enzymes, and significantly enhance the lightness of the treated product. However, the IR heating alone could not achieve the 5 log reduction and very light color of dried products.
[0027] Figures 10a, 10b, 11, and 12 illustrate the results of experiments that utilized sequential treatments of food products utilizing ozone treatment and IR heating. Experimental results of the sequential utilization of ozone treatment and IR heating with the use of ozone treatment only and IR heating only.
[0028] In the experiments conducted, the food product (e.g., grapes) were treated with ozone under different gaseous ozone concentrations ranging from 200 to 800 ppm for durations of time ranging from 60 and 120 min, respectively. After ozone treatment, the samples were heated using infrared (IR) for 5 min. After sequential ozone treatment and IR heating, the samples were dried using hot air drying at 60 °C to a final moisture content of 10+1.0% (w.b). The drying time was recorded and compared with that of untreated samples. The microbial load was evaluated right after sequential ozone and IR heating treatment. The color of the dried grapes was measured after hot air drying. The combination of ozone treatment at 800 ppm for 60 and 120 min with IR heating for 5 min reduced the E. faecium by 4.5 and 5.18 log CFU/g, respectively (Figure 10B). Additionally, the sequential ozone and IR heating treatment significantly brightened the color of dried grapes. The values of L* as an indicator of brightness increased from 36.54+3.74 to 41.49+3.69 by increasing the zone concentration from 200 to 800 ppm and treatment time from 60 min to 120 min, respectively, (Table 3, below). The results indicate the sequential ozone treatment and IR heating was able to effectively inactivate the browning enzymes and produce dried grape with light color (Figure 11). It took 48 hr for the hot air drying to dry the slices treated with sequential ozone treatment at 800 ppm for 120 min and IR heating for 5 min, while the corresponding hot air drying for untreated samples was 72 hr. This indicates that the use of IR heating reduced the hot air drying time by 33%.
Figure imgf000011_0001
TABLE 3
[0029] Based on the discovered findings, the embodiments of the present technology of using sequential ozone and IR heating treatment as a pre-treatment method of hot air drying provide the following alternative variants to the previous arts. Ozone treatment has high antimicrobial capacity and can effectively destroy microorganisms and slightly improve the color of treated product by reducing the activities of enzymes causing browning. IR has high heating rate and powerful thermal inactivation capability of microorganisms and the enzymes causing browning and significantly enhances the lightness of the treated product and reduces the drying time for the hot air drying. Separate ozone treatment or IR heating alone cannot achieve 4 to 5 log reduction of microbial load and cannot produce the dried products with very light color. The sequential ozone and IR heating treatment as a pre-treatment method of hot air had positive synergetic effect on reduction of microbial load and product color change during drying process. It can achieve more than 5 log reduction of microbial load that ensured food safety, and produce the dried products with very light color, the new processing method also can reduce the drying time of hot air drying. Sequential ozone treatment and IR heating can achieve similar functions of Sulfur dioxide (SO2) treatment in fruit drying process and produce dried fruits with ensured safety and light color without any health and environmental concerns.
Discussion of Possible Embodiments [0030] The following are non-exclusive descriptions of possible embodiments of the present invention
[0031] A method of treating food products includes applying an ozone treatment to the food product, wherein the ozone treatment includes exposing the food product to a gaseous ozone environment. The method further includes applying infrared (IR) heating to the ozone treated food product.
[0032] The method of the preceding paragraph ca optionally include, additionally and/or alternatively any, one or more of the following features, configurations and/or additional components
[0033] For example, the method may include drying the food product following infrared heating utilizing a hot air dryer, wherein IR heating of the ozone treated food product reduces the drying time of the food product.
[0034] The method may further include wherein the gaseous ozone environment has an ozone concentration greater than 200 parts per million (ppm).
[0035] The method may further include wherein the gaseous ozone environment has an ozone concentration greater than 400 ppm.
[0036] The method may further include wherein the gaseous ozone environment has an ozone concentration greater than 600 ppm.
[0037] The method may further include wherein the gaseous ozone environment has an ozone concentration greater than 800 ppm.
[0038] The method may further include wherein the ozone treatment is applied for a duration of at least 30 minutes.
[0039] The method may further include wherein the ozone treatment is applied for a duration of at least 60 minutes.
[0040] The method may further include wherein the ozone treatment is applied for a duration of at least 90 minutes.
[0041] The method may further include wherein the ozone treatment is applied for a duration of at least 120 minutes.
[0042] The method may further include wherein the IR heating is applied for a duration of at least one minute. [0043] The method may further include wherein the IR heating is applied for a duration of at least two minutes.
[0044] The method may further include wherein the IR heating is applied for a duration of at least three minutes.
[0045] The method may further include wherein the IR heating is applied for a duration of at least four minutes.
[0046] The method may further include wherein the IR heating is applied for a duration of at least five minutes.
[0047] The method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 50° Celsius (C).
[0048] The method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 60° Celsius (C).
[0049] The method may further include wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 70° Celsius (C).
[0050] According to another aspect, a food processing apparatus may include an ozone treatment apparatus including an ozone chamber for exposing a food product to a gaseous ozone environment and an infrared (IR) heating apparatus including infrared heating elements for heating the ozone treated food product.
[0051] The food processing apparatus of the preceding paragraph can optionally include, additionally and/or alternatively any, one or more of the following features, configurations and/or additional components.
[0052] For example, the apparatus may further include a hot air drying apparatus configured to dry the ozone treated and IR heated food product.
[0053] In some embodiments, the ozone treatment apparatus may include an ozone analyzer that measures ozone concentrations in the ozone chamber.
[0054] In some embodiments, the IR heating element includes a temperature monitoring device that measures one of IR heating apparatus air temperature, food product surface temperature, and/or interior temperature of the food product.

Claims

1. A method of treating food products, the method comprising: applying an ozone treatment to the food product, wherein the ozone treatment includes exposing the food product to a gaseous ozone environment; and applying infrared (IR) heating to the ozone treated food product.
2. The method of claim 1, further including: drying the food product following infrared heating utilizing a hot air dryer, wherein IR heating of the ozone treated food product reduces the drying time of the food product.
3. The method of any preceding claim, wherein the gaseous ozone environment has an ozone concentration greater than 200 parts per million (ppm).
4. The method of any preceding claim, wherein the gaseous ozone environment has an ozone concentration greater than 400 ppm.
5. The method of any preceding claim, wherein the gaseous ozone environment has an ozone concentration greater than 600 ppm.
6. The method of any preceding claim, wherein the gaseous ozone environment has an ozone concentration greater than 800 ppm.
7. The method of any preceding claim, wherein the ozone treatment is applied for a duration of at least 30 minutes.
8. The method of any preceding claim, wherein the ozone treatment is applied for a duration of at least 60 minutes.
9. The method of any preceding claim, wherein the ozone treatment is applied for a duration of at least 90 minutes.
10. The method of any preceding claim, wherein the ozone treatment is applied for a duration of at least 120 minutes.
11. The method of any preceding claim, wherein the IR heating is applied for a duration of at least one minute.
12. The method of any preceding claim, wherein the IR heating is applied for a duration of at least two minutes.
13. The method of any preceding claim, wherein the IR heating is applied for a duration of at least three minutes.
14. The method of any preceding claim, wherein the IR heating is applied for a duration of at least four minutes.
15. The method of any preceding claim, wherein the IR heating is applied for a duration of at least five minutes.
16. The method of any preceding claim, wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 50° Celsius (C).
17. The method of any preceding claim, wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 60° Celsius (C).
18. The method of any preceding claim, wherein the IR heating includes heating an internal temperature of the food product to a temperature of greater than 70° Celsius (C).
19. A food processing apparatus comprising: an ozone treatment apparatus including an ozone chamber for exposing a food product to a gaseous ozone environment; and an infrared (IR) heating apparatus including infrared heating elements for heating the ozone treated food product.
20. The food processing apparatus of claim 19, further including: a hot air drying apparatus configured to dry the ozone treated and IR heated food product.
21. The food drying apparatus of claim 19, wherein the ozone treatment apparatus includes an ozone analyzer that measures ozone concentrations in the ozone chamber.
22. The food processing apparatus of claim 19, wherein the IR heating element includes a temperature monitoring device that measures one of IR heating apparatus air temperature, food product surface temperature, and/or interior temperature of the food product.
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