WO2021255263A3 - Procédé multiplex pour détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon - Google Patents

Procédé multiplex pour détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon Download PDF

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Publication number
WO2021255263A3
WO2021255263A3 PCT/EP2021/066668 EP2021066668W WO2021255263A3 WO 2021255263 A3 WO2021255263 A3 WO 2021255263A3 EP 2021066668 W EP2021066668 W EP 2021066668W WO 2021255263 A3 WO2021255263 A3 WO 2021255263A3
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Prior art keywords
analyte
variations
sample
different
multiplex method
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PCT/EP2021/066668
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English (en)
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WO2021255263A2 (fr
Inventor
Andreas Geipel
Frank Reinecke
Christian Korfhage
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Resolve Biosciences Gmbh
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Priority claimed from PCT/EP2020/067010 external-priority patent/WO2020254519A1/fr
Application filed by Resolve Biosciences Gmbh filed Critical Resolve Biosciences Gmbh
Priority to CN202180041893.2A priority Critical patent/CN115702249A/zh
Priority to AU2021294274A priority patent/AU2021294274A1/en
Priority to JP2022575771A priority patent/JP2023530889A/ja
Priority to KR1020227043398A priority patent/KR20230023647A/ko
Priority to EP21733989.4A priority patent/EP4168576A2/fr
Priority to CA3181737A priority patent/CA3181737A1/fr
Priority to US18/001,930 priority patent/US20240279736A1/en
Priority to US17/554,093 priority patent/US20220205028A1/en
Publication of WO2021255263A2 publication Critical patent/WO2021255263A2/fr
Publication of WO2021255263A3 publication Critical patent/WO2021255263A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

La technologie de la présente invention concerne des procédés multiplex et des kits permettant de détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon, ainsi que des procédés in vitro pour cribler, identifier et/ou tester une substance et/ou un médicament et des procédés in vitro pour le diagnostic d'une maladie, ainsi qu'un système de multiplexage optique.
PCT/EP2021/066668 2020-06-18 2021-06-18 Procédé multiplex pour détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon WO2021255263A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CN202180041893.2A CN115702249A (zh) 2020-06-18 2021-06-18 用于检测样品中的不同分析物以及分析物的不同亚组/变异体的多重方法
AU2021294274A AU2021294274A1 (en) 2020-06-18 2021-06-18 Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample
JP2022575771A JP2023530889A (ja) 2020-06-18 2021-06-18 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法
KR1020227043398A KR20230023647A (ko) 2020-06-18 2021-06-18 샘플에서 상이한 분석물 및 분석물의 상이한 하위군/변형을 검출하기 위한 멀티플렉스 방법
EP21733989.4A EP4168576A2 (fr) 2020-06-18 2021-06-18 Procédé multiplex pour détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon
CA3181737A CA3181737A1 (fr) 2020-06-18 2021-06-18 Procede multiplex pour detecter differents analytes et differents sous-groupes/variations d'un analyte dans un echantillon
US18/001,930 US20240279736A1 (en) 2020-06-18 2021-06-18 Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample
US17/554,093 US20220205028A1 (en) 2020-12-18 2021-12-17 Multiplex method for detecting different analytes in a sample

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
PCT/EP2020/067010 WO2020254519A1 (fr) 2019-06-18 2020-06-18 Procédé de codage de signal d'analytes dans un échantillon
EPPCT/EP2020/067010 2020-06-18
US202063127910P 2020-12-18 2020-12-18
US63/127,910 2020-12-18
US202063129942P 2020-12-23 2020-12-23
US63/129,942 2020-12-23

Related Child Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2021/066620 Continuation WO2021255244A1 (fr) 2020-06-18 2021-06-18 Procédé multiplex de détection d'analytes différents dans un échantillon

Publications (2)

Publication Number Publication Date
WO2021255263A2 WO2021255263A2 (fr) 2021-12-23
WO2021255263A3 true WO2021255263A3 (fr) 2022-02-03

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PCT/EP2021/066668 WO2021255263A2 (fr) 2020-06-18 2021-06-18 Procédé multiplex pour détecter différents analytes et différents sous-groupes/variations d'un analyte dans un échantillon
PCT/EP2021/066620 WO2021255244A1 (fr) 2020-06-18 2021-06-18 Procédé multiplex de détection d'analytes différents dans un échantillon

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/EP2021/066620 WO2021255244A1 (fr) 2020-06-18 2021-06-18 Procédé multiplex de détection d'analytes différents dans un échantillon

Country Status (8)

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US (3) US20240279736A1 (fr)
EP (2) EP4168576A2 (fr)
JP (2) JP2023530889A (fr)
KR (2) KR20230023646A (fr)
CN (2) CN115702249A (fr)
AU (2) AU2021291051A1 (fr)
CA (2) CA3181737A1 (fr)
WO (2) WO2021255263A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022131441A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131448A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131451A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131445A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131444A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131446A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131442A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Zuordnen von Bildbereichen einer Bildfolge zu Ergebnisklassen mittels eines Analytdaten-Auswertesystems mit einem Verarbeitungsmodells
DE102022131450A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131449A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
WO2024179837A1 (fr) * 2023-03-01 2024-09-06 Resolve Biosciences Gmbh Formation complexe pour la détection parallèle de multiples analytes dans de multiples applications
WO2024256715A1 (fr) * 2023-06-16 2024-12-19 Resolve Biosciences Gmbh Réactif pour le contrôle qualité de l'hybridation in situ

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017189525A1 (fr) * 2016-04-25 2017-11-02 President And Fellows Of Harvard College Procédés de réaction en chaîne d'hybridation pour la détection moléculaire in situ

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1228811A (fr) 1983-05-05 1987-11-03 Robert G. Pergolizzi Methode d'analyse utilisant des sequences de polynucleotides
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
EP2992115B1 (fr) 2013-04-30 2020-03-04 California Institute of Technology Marquage multiplex de molécules par marquage par code-barres à hybridation séquentielle
CN109804084A (zh) * 2016-08-01 2019-05-24 加州理工学院 基于具有嵌入纠错机制的伪彩色条形码的分子靶的顺序探测
EP3894583A4 (fr) * 2018-12-13 2022-11-09 President and Fellows of Harvard College Systèmes et procédés d'amplification pour merfish et d'autres applications

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017189525A1 (fr) * 2016-04-25 2017-11-02 President And Fellows Of Harvard College Procédés de réaction en chaîne d'hybridation pour la détection moléculaire in situ

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HARRY M. T. CHOI ET AL: "Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust", DEVELOPMENT (SPECIAL ISSUE ON DEVELOPMENT AT THE SINGLE CELL LEVEL), vol. 145, no. 12, 15 June 2018 (2018-06-15), GB, XP055590305, ISSN: 0950-1991, DOI: 10.1242/dev.165753 *
KARUNANAYAKE MUDIYANSELAGE ARUNI P K K ET AL: ""Second-generation" fluorogenic RNA-based sensors", METHODS, vol. 161, 17 January 2019 (2019-01-17), pages 24 - 34, XP085713798, ISSN: 1046-2023, DOI: 10.1016/J.YMETH.2019.01.008 *
SUN Z, NGUYEN T, MCAULIFFE K, YOU M: "Intracellular Imaging with Genetically Encoded RNA-based Molecular Sensors.", NANOMATERIALS (BASEL), vol. 9, 8 February 2019 (2019-02-08), XP002795696 *

Also Published As

Publication number Publication date
CN115698322A (zh) 2023-02-03
WO2021255244A1 (fr) 2021-12-23
KR20230023647A (ko) 2023-02-17
US20230227907A1 (en) 2023-07-20
KR20230023646A (ko) 2023-02-17
EP4168576A2 (fr) 2023-04-26
CA3181737A1 (fr) 2021-12-23
US20220205028A1 (en) 2022-06-30
AU2021294274A1 (en) 2022-12-08
EP4168575A1 (fr) 2023-04-26
CN115702249A (zh) 2023-02-14
US20240279736A1 (en) 2024-08-22
JP2023529682A (ja) 2023-07-11
AU2021291051A1 (en) 2022-12-08
JP2023530889A (ja) 2023-07-20
CA3181725A1 (fr) 2021-12-23
WO2021255263A2 (fr) 2021-12-23

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