WO2021093885A9 - 同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒 - Google Patents

同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒 Download PDF

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WO2021093885A9
WO2021093885A9 PCT/CN2020/129073 CN2020129073W WO2021093885A9 WO 2021093885 A9 WO2021093885 A9 WO 2021093885A9 CN 2020129073 W CN2020129073 W CN 2020129073W WO 2021093885 A9 WO2021093885 A9 WO 2021093885A9
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diacetoxyscirpenol
sterigmatocystin
aflatoxin
detection
time
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WO2021093885A1 (zh
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李培武
李慧
姜俊
张文
张奇
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中国农业科学院油料作物研究所
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Publication of WO2021093885A1 publication Critical patent/WO2021093885A1/zh
Publication of WO2021093885A9 publication Critical patent/WO2021093885A9/zh

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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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Abstract

同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素B1、杂色曲霉素的时间分辨荧光试剂盒，包括免疫层析时间分辨荧光试纸条和含有铕标记的抗二乙酸镳草镰刀菌烯醇、黄曲霉毒素B1、杂色曲霉素单克隆抗体的样品反应瓶，免疫层析时间分辨荧光试纸条的一面从上至下依次设置吸水垫（1）、检测垫（2）和样品垫（3），相邻各垫于连接处交叠相接，检测垫（2）以硝酸纤维素膜为基垫，硝酸纤维素膜上自上而下横向设有质控线（4）和检测线（5,6,7），质控线（4）包被有兔抗鼠多克隆抗体，检测线（5,6,7）个数为三条，分别包被各毒素-蛋白偶联物。能实现对二乙酸镳草镰刀菌烯醇、黄曲霉毒素B1、杂色曲霉素三种真菌毒素的快速、同步检测。
PCT/CN2020/129073 2019-11-15 2020-11-16 同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒 WO2021093885A1 (zh)

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US17/777,269 US11971403B2 (en) 2019-11-15 2020-11-16 Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1, and sterigmatocystin

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CN201911121513.7A CN110806481B (zh) 2019-11-15 2019-11-15 同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒
CN201911121513.7 2019-11-15

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WO2021093885A9 true WO2021093885A9 (zh) 2021-06-17

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CN110806481B (zh) * 2019-11-15 2021-09-21 中国农业科学院油料作物研究所 同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒
CN111505293B (zh) * 2020-04-03 2022-11-18 北京望尔生物技术有限公司 一种检测杂色曲霉素的试纸条及其应用
CN115166231A (zh) * 2021-11-26 2022-10-11 国家食品安全风险评估中心 桔青霉素的侧流免疫层析方法

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US20110229914A1 (en) * 2009-10-21 2011-09-22 Frances Eun-Hyung Lee Use of Antibody Secreting Cell Elispot To Assess Antibody Responses Following Antigen Exposure
CN101930006B (zh) * 2010-08-05 2012-08-22 中国农业科学院油料作物研究所 快速检测黄曲霉毒素总量的高灵敏度免疫层析试纸条及其制备方法
CN101993855B (zh) 2010-08-05 2012-06-27 中国农业科学院油料作物研究所 杂交瘤细胞株1c11、其产生的抗黄曲霉毒素通用单克隆抗体及其应用
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CN110806481B (zh) 2019-11-15 2021-09-21 中国农业科学院油料作物研究所 同步检测二乙酸镳草镰刀菌烯醇、黄曲霉毒素b1、杂色曲霉素的时间分辨荧光试剂盒
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CN110806481B (zh) 2021-09-21
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CN110806481A (zh) 2020-02-18
WO2021093885A1 (zh) 2021-05-20

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