WO2021087551A1 - A method to produce targeted gene editing constructs - Google Patents
A method to produce targeted gene editing constructs Download PDFInfo
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- WO2021087551A1 WO2021087551A1 PCT/AU2020/051102 AU2020051102W WO2021087551A1 WO 2021087551 A1 WO2021087551 A1 WO 2021087551A1 AU 2020051102 W AU2020051102 W AU 2020051102W WO 2021087551 A1 WO2021087551 A1 WO 2021087551A1
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- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/28—Cannabaceae, e.g. cannabis
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Definitions
- Figure 9 is a photographic representation of mechanically filtrated, digested leaf protoplasts and associated successive filtration steps to remove remaining plant debris.
- Cannabinoid biosynthesis genes were accessed from a variety of sources and public databases (Table 3). Sequences were downloaded and used as a query for BLAST analysis against the genome assembly with an e-value threshold set at ⁇ 10-10. Identified scaffold regions of interest from the reference genome were annotated and visualised using FGENESH (Solovyev etal., 2006, Genome Biology, 7(1): S10) and MEGANTE (Numa and Itoh, 2013, Plant and Cell Physiology, 55(1): e2-e2).
- the transformation buffer was prepared fresh for every transformation. 30mL aliquots of the transformation buffer were prepared, pH balanced and fdter sterilised through 0.22 pm fdter syringe.
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Abstract
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WO2023035035A1 (en) * | 2021-09-09 | 2023-03-16 | Agriculture Victoria Services Pty Ltd | Methods for the modification of cells, modified cells and uses thereof |
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WO2018035450A1 (en) * | 2016-08-18 | 2018-02-22 | Ebbu, LLC | Plants and methods for increasing and decreasing synthesis of cannabinoids |
WO2020035869A1 (en) * | 2018-08-17 | 2020-02-20 | Canbreed Ltd. | Modulation of cannabinoid profile in cannabis |
WO2020170251A1 (en) * | 2019-02-23 | 2020-08-27 | Canbreed Ltd. | Powdery mildew resistant cannabis plants |
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US9095554B2 (en) * | 2013-03-15 | 2015-08-04 | Biotech Institute LLC | Breeding, production, processing and use of specialty cannabis |
RU2017146186A (en) * | 2015-05-28 | 2019-06-28 | Твид Инк. | KONOPLI PLANTS WITH MODIFIED THCA-SYNTHASE EXPRESSION |
US20200347394A1 (en) * | 2017-11-02 | 2020-11-05 | Yield10 Bioscience, Inc. | Genes and gene combinations for enhanced crops |
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WO2018035450A1 (en) * | 2016-08-18 | 2018-02-22 | Ebbu, LLC | Plants and methods for increasing and decreasing synthesis of cannabinoids |
WO2020035869A1 (en) * | 2018-08-17 | 2020-02-20 | Canbreed Ltd. | Modulation of cannabinoid profile in cannabis |
WO2020170251A1 (en) * | 2019-02-23 | 2020-08-27 | Canbreed Ltd. | Powdery mildew resistant cannabis plants |
Non-Patent Citations (3)
Title |
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ARNDELL, T. ET AL.: "gRNA validation for wheat genome editing with the CRISPR-Cas9 system", BMC BIOTECHNOLOGY, vol. 19, no. 71, 30 October 2019 (2019-10-30), pages 1 - 12, XP055822047 * |
LI, J. ET AL.: "Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants", PLANT BIOTECHNOLOGY JOURNAL, vol. 17, no. 5, 6 October 2018 (2018-10-06), pages 858 - 868, XP055822051 * |
See also references of EP4055172A4 * |
Cited By (1)
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WO2023035035A1 (en) * | 2021-09-09 | 2023-03-16 | Agriculture Victoria Services Pty Ltd | Methods for the modification of cells, modified cells and uses thereof |
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IL292540A (en) | 2022-06-01 |
CA3155707A1 (en) | 2021-05-14 |
US20230125534A1 (en) | 2023-04-27 |
AU2020378910A1 (en) | 2022-06-16 |
EP4055172A1 (en) | 2022-09-14 |
EP4055172A4 (en) | 2024-06-12 |
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