WO2020120808A1 - Dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires - Google Patents

Dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires Download PDF

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Publication number
WO2020120808A1
WO2020120808A1 PCT/ES2018/070797 ES2018070797W WO2020120808A1 WO 2020120808 A1 WO2020120808 A1 WO 2020120808A1 ES 2018070797 W ES2018070797 W ES 2018070797W WO 2020120808 A1 WO2020120808 A1 WO 2020120808A1
Authority
WO
WIPO (PCT)
Prior art keywords
sensor
support body
detection device
food product
layers
Prior art date
Application number
PCT/ES2018/070797
Other languages
English (en)
Spanish (es)
Inventor
Sergio Arana Alonso
Eva PÉREZ LORENZO
Maite MUJIKA GARMENDIA
Andreina Alexandra MORALES RONDÓN
Luís María SANCHO SEUMA
Javier GARCÍA GOIKOETXEA
Original Assignee
Asociacion Centro Tecnologico Ceit-Ik4
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asociacion Centro Tecnologico Ceit-Ik4 filed Critical Asociacion Centro Tecnologico Ceit-Ik4
Priority to PCT/ES2018/070797 priority Critical patent/WO2020120808A1/fr
Publication of WO2020120808A1 publication Critical patent/WO2020120808A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance

Definitions

  • the present invention is related to a device for detecting bacterial bi-layers in a food product manufacturing pipeline, comprising a conduit section for receiving the food product, in which a sensor is arranged that detects the formation of bacterial bi-layers, so that when the bacterial bi-layers are detected, the food product pipeline is cleaned.
  • Bacterial biolayers are complex and heterogeneous biological structures made up of one or more species of bacteria housed within an extracellular matrix. Although there are cases in which the biolayers are formed by a single bacterial species, in nature they generally develop from the aggregation of different bacterial types. Likewise, other types of organisms also act in the formation, such as fungi, algae, protozoa, yeasts, wastes and corrosive products.
  • EPS Extracellular Polymeric Substances
  • the invention relates to a device for detecting bacterial bi-layers in a pipe for manufacturing a food product, such as an edible paste, milk, water, or beverages of any kind, without being these limiting examples.
  • the bacterial biolayer detection device comprises a conduit section to receive the food product, which has ends configured to be coupled to the food product pipeline, so that, in use, the food product circulates inside the conduction section of the detection device.
  • the detection device comprises a sensor that allows the detection of bacterial bi-layers.
  • Said sensor is an impedimetric sensor in which the bacteria present in the food product are fixed, which modifies the variation of the electrical impedance of the sensor and thus allows detecting the formation of bacterial bi-layers in the sensor. For this reason, the cleaning of the interior of the food product pipeline is only carried out when bacterial bi-layers are detected, which entails significant economic savings since the production cycle is stopped only when it is really necessary to undertake the cleaning of the pipeline.
  • the sensor is arranged in a housing of the conduction section, such that in use the sensor is arranged in contact with the food product, so that the bacteria present in the food product can become attached to the sensor and thus detect the formation of bacterial bi-layers .
  • the sensor is flush or in line with an interior wall of the conduction section, since the formation of bacterial bi-layers is more prone to the points closest to the interior wall of the conduction section.
  • the conduit section comprises a branch in which a support body for the sensor arrangement is arranged, which allows the sensor to be stably arranged in the conduction section and can also be easily removed by removing the support body of branching.
  • the support body has a first end in which the sensor is arranged, housed in the housing, and having a cylindrical concave configuration identical to the shape of the inner wall of the conduit section. Thanks to this configuration, section changes or elements that at least partially block the flow of the food product inside the conduit section are avoided, since the formation of bacterial bi-layers is more prone to points close to section changes in the pipeline or to elements that partially block the flow of the food product.
  • the support body includes through openings for the arrangement inside of cables that are attached to the sensor, so that these cables transmit signals of the electrical impedance of the sensor to a processor where these signals are translated into digital values and the formation or not of bacterial bio-layers can be interpreted through these values.
  • the support body comprises a handle element to facilitate the removal of the support body from inside the branch when the support body is in its use position, which is only necessary to replace the sensor at the end of its useful life or when a failure is detected in the detection device and it is necessary to remove the support body and try to remedy the failure produced in the elements that are arranged on the support body.
  • the detection device comprises a cover that is arranged to close a second end of the branch, an end that is not coupled to the conduit section.
  • Said cover comprises at least one through hole in correspondence with a blind hole in the support body, through which a pin is inserted. In this way, when the pin is inserted between the through hole and the blind hole in correspondence, the rotation of the support body is impossible, so that the end of the support body with a cylindrical concave shape is flush with the inner wall of the section conduction and there are no elements that partially block the passage of the food product.
  • the cover comprises two through holes and the support body two blind holes in correspondence with the through holes of the cover, through which two pins are inserted, one for each through hole and blind hole, providing greater security and precision. to the stopping device.
  • the detection device comprises a temperature sensor to determine the temperature of the food product.
  • the electrical impedance in the sensor for the detection of bacterial bi-layers is also modified by alterations in the temperature of the food product, so the temperature sensor allows knowing the temperature in the food product and applying a correction factor to the values of electrical impedance obtained from the sensor in case of changes in the temperature of the food product.
  • the temperature sensor is a thermocouple housed in a through hole from the support body, a first end of the temperature sensor being in contact with the food product and a second end of the temperature sensor connected to the processor, where the signals obtained from the temperature of the food product are translated into digital values.
  • a sealing gasket that prevents the food product from flowing out through the conduit section through the branch.
  • the invention is a device for detecting bacterial bi-layers in food product pipes, which allows the cleaning of the interior of these pipes to be carried out only when the formation of bacterial bi-layers is detected.
  • Figure 1 shows a perspective view of an embodiment of the bacterial biolayer detection device.
  • Figure 2 shows a sectional view of the detection device object of the invention.
  • Figure 3 shows an exploded perspective view of the detection device of the previous figures.
  • the invention relates to a device for detecting bacterial bi-layers for its arrangement in a food product pipeline, comprising a conduit section (1) for receiving the food product, said conduit section comprising (1) ends (1.1, 1.2) configured to be coupled to the food products pipeline.
  • Said coupling can be produced by any joining means, such as welding, as long as the coupling between the conduit section (1) and the pipe is a watertight coupling that prevents part of the food product from escaping.
  • the bacterial biolayer detection device object of the invention It comprises a sensor (2) capable of detecting a colony of bacteria that can lead to the formation of bacterial biofilms, harmful to human consumption.
  • the sensor (2) is an impedimetric sensor on which bacteria from the food product are attached and which is used to detect variations in electrical impedance in the sensor (2) due to the deposition of bacteria on the sensor (2) .
  • the senor (2) comprises a matrix of interdigitated microelectrodes (2.1) and two contact pins (2.2), arranged on the sides of said matrix of interdigitated microelectrodes (2.1).
  • the sensor (2) is arranged in a housing (3) in the conduit section (1), so that the sensor (2) is in contact with the food product and the bacteria in the food product can become attached to the sensor (2), so that the formation of bacterial bi-layers is detected.
  • the sensor (2) located in its housing (3), is flush or in line with an interior wall (1.3) of the conduit section (1), so that the presence of the sensor (2) inside the conduction section (1) does not modify the flow of the food product through conduction section (1).
  • a change of section or an element that gets in the flow of the food product would modify this flow and create a turbulent flow, at least in the points near the change of section or blocking element, which would cause the formation of bacterial bi-layers.
  • the detection device object of the invention comprises a branch (4) projecting from the conduit section (1), preferably in an inclined manner, with a first end (4.1) being connected to the conduction section (1) and a second extreme (4.2) opposite free.
  • a support body (5) is arranged, which in turn comprises a first end (5.1) in which the housing (3) for the arrangement of the sensor (2) is arranged and a second opposite end (5.2).
  • the support body (5) is arranged inside the branch (4) so that the support body (5) occupies the entire interior section of the branch (4), preventing the outflow of food product by the branch ( 4).
  • the first end (5.1) of the support body (5) has a cylindrical concave configuration identical to the shape of the interior wall (1.3) of the conduction section (1). Thanks to this configuration, changes of section or elements that at least partially block the flow of the food product, since, as previously mentioned, the formation of bacterial bi-layers is more prone to points close to section changes or elements that block the flow.
  • the support body (5) includes through openings (5.3), inside which cables (6) are attached to the contact pins (2.2) of the sensor (2).
  • the cables (6) are attached by soldering with tin to said contact pins (2.2) of the sensor (2).
  • the cables (6) are joined at the other end to a processor where signals received from the electrical impedance in the sensor (2) are translated into digital values.
  • the support body (5) comprises a handle element (7) that allows the removal of the support body (5) from the interior of the branch (4).
  • the handle element (7) is threaded to the end (5.2) of the support body (5), so that by pulling the handle element (7) it is possible to extract the support body (5) from inside the branch (4).
  • the detection device additionally comprises a cover (8) that allows the closure of the free end (4.2) of the branch (4), attaching the cover (8) to said end (4.2) of the branch (4).
  • a cover (8) that allows the closure of the free end (4.2) of the branch (4), attaching the cover (8) to said end (4.2) of the branch (4).
  • the connection of the cover (8) to the branch (4) is carried out by means of screws (8.1).
  • Said cover (8) comprises at least one through hole (8.2) through which a pin (9) is inserted.
  • the support body (5) comprises at least one blind hole (5.4) in correspondence with said through hole (8.2), in which the pin (9) is also inserted.
  • the rotation of the support body (5) is impossible, so that the end (5.1) of the Support body (5) with a cylindrical concave configuration is flush with the interior wall (1.3) of the conduction section (1) and there are no elements that partially block the passage of the food product.
  • the cover (8) comprises two through holes (8.2) and the support body (5) two blind holes (5.4) in correspondence with the through holes (8.2), for the introduction of two pins (9), one for each through hole (8.2) and blind hole (5.4), providing greater security and precision to the arresting device.
  • the detection device comprises stop means so that the support body (5) enters the branch (4) to a specific depth in which the end (5.1) of the support body (5) remains flush or in line with the interior wall (1.3) of the conduit section (1), so that the passage of the food product is not partially blocked.
  • Said means may be a stop arranged between the support body (5) and the interior of the branch (4) (not represented in the figures), without this limiting embodiment of the invention.
  • the detection device comprises a temperature sensor (10) that allows determining the temperature of the food product that circulates inside the conduit section (1).
  • the temperature sensor (10) is a thermocouple that is arranged in a through hole (5.5) of the support body (5), with one end of the temperature sensor (10) being in contact with the food product and the end Opposite connected to a processor where signals received by the temperature sensor (10) are convertible into digital values.
  • the sealing gasket (11) is arranged as close as possible to the end (5.1) of the support body (5), so that the minimum amount of food product is introduced inside the branch (4) .
  • the support body (5) is arranged inside the branch (4), the sensor (2) remaining in the housing (3) and in contact with the food product that circulates inside the conduction section (1).
  • An electrical signal is passed through the sensor (2) without interruption, obtaining a reference value of electrical impedance.
  • bacteria begin to exist in the flow of the food product, some of these bacteria remain stuck in the matrix of interdigitated microelectrodes (2.1), beginning to form a bacterial biolayer.
  • the bacterial biolayer formed in the matrix of interdigitated microelectrodes (2.1) modifies the value of the electrical impedance obtained in the sensor (2), thus being able to detect the presence of bacterial bi-layers by a variation in the electrical impedance of the sensor (2).
  • the temperature sensor (10) allows the temperature in the food product to be known, so that in case of changes in temperature of the food product, in the processor a correction factor can be applied to the electrical impedance values obtained from the sensor (2).
  • the cleaning of the interior of this pipe is carried out by the CIP method (“Clean in place); In other words, cleaning is done without disassembling or opening the parts that make up the pipe.
  • the production cycle of the food product is paralyzed and water and different chemical agents are passed through the pipeline.
  • the CIP cleaning method comprises firstly washing the pipeline by introducing water under pressure, as a second step, introducing a chemical agent (such as caustic soda, nitric acid or a detergent) and as a third step, again washing the pipeline with water at pressure to remove the chemical agents introduced in the second step.
  • a chemical agent such as caustic soda, nitric acid or a detergent
  • the said second and third steps can be repeated by modifying the chemical agent introduced.
  • the sensor (2) is washed after cleaning by the CIP method described above, since in this way the bacteria attached to the matrix of interdigitated microelectrodes (2.1) are eliminated.
  • the support body (5) is reintroduced inside the branch (4). So that the first end (5.1) of the support body (5) with a cylindrical concave is flush with the inner wall (1.3) of the conduction section (1) and said first end (5.1) does not partially block the passage of the food product , the pins (9) are inserted between the through holes (8.2) of the cover (8) and the blind holes (5.4) of the support body (5), leaving the support body (5) in its correct position and making it impossible the rotation of the support body (5).
  • the stop means will cause the support body (5) to enter the branch (4) up to the depth at which the end (5.1) of the support body (5) is flush or in line with the inner wall (1.3) of the driving section (1).
  • the detection device object of the invention is arranged in the pipe after a section change thereof (such as an elbow) or after an element that partially blocks the passage of the food fluid, since as already has commented previously, in these places it is where the formation of bacterial bi-layers is more prone.
  • a section change thereof such as an elbow
  • an element that partially blocks the passage of the food fluid since as already has commented previously, in these places it is where the formation of bacterial bi-layers is more prone.
  • the detection device object of the invention is attachable to an already installed and operational food processing pipeline, for which the ends (1.1, 1.2) of the conduction section (1) must be coupled to the pipeline, so that the flow of food product circulates through the detection device.
  • the detection device can be installed as a starting point in a new installation of a food processing pipeline, also coupling the ends (1.1, 1.2) of the conduit section (1) to the pipeline during the initial installation.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires, comme par exemple une pâte comestible, du lait, de l'eau ou des boissons de tout type, qui comprend un segment de conduite (1) pour recevoir le produit alimentaire, qui comporte deux extrémités (1.1, 1.2) configurées pour accoupler le segment de conduite (1) au tube, et un capteur (2) pour la détection de biocouches bactériennes préjudiciables à la consommation par l'Homme, ce qui permet de réaliser le nettoyage de l'intérieur du tube uniquement losque la formation de biocouches bactériennes est détectée.
PCT/ES2018/070797 2018-12-11 2018-12-11 Dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires WO2020120808A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/ES2018/070797 WO2020120808A1 (fr) 2018-12-11 2018-12-11 Dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2018/070797 WO2020120808A1 (fr) 2018-12-11 2018-12-11 Dispositif de détection de biocouches bactériennes sur un tube de production de produits alimentaires

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WO2020120808A1 true WO2020120808A1 (fr) 2020-06-18

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040228953A1 (en) * 2001-12-14 2004-11-18 Bigalke Darreli Lee Continuous fluid sampler and method
US20050059105A1 (en) * 2003-07-25 2005-03-17 Board Of Trustees Of Michigan State University Impedimetric biosensor and its use for rapid detection of bacterial pathogens in solution
EP1972198A1 (fr) * 2007-03-12 2008-09-24 Ali SpA Machine et procédé pour la production et la distribution de produits alimentaires liquides et semi-liquides
ITBO20100233A1 (it) * 2010-04-15 2011-10-16 Carpigiani Group Ali Spa Dispositivo di controllo della carica batterica di un prodotto alimentare liquido o semiliquido.
EP2491792A1 (fr) * 2011-02-28 2012-08-29 ALI S.p.A. - Carpigiani Group Machine pour la fabrication et la distribution de produits alimentaires liquides, semi-liquides et/ou semi-solides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040228953A1 (en) * 2001-12-14 2004-11-18 Bigalke Darreli Lee Continuous fluid sampler and method
US20050059105A1 (en) * 2003-07-25 2005-03-17 Board Of Trustees Of Michigan State University Impedimetric biosensor and its use for rapid detection of bacterial pathogens in solution
EP1972198A1 (fr) * 2007-03-12 2008-09-24 Ali SpA Machine et procédé pour la production et la distribution de produits alimentaires liquides et semi-liquides
ITBO20100233A1 (it) * 2010-04-15 2011-10-16 Carpigiani Group Ali Spa Dispositivo di controllo della carica batterica di un prodotto alimentare liquido o semiliquido.
EP2491792A1 (fr) * 2011-02-28 2012-08-29 ALI S.p.A. - Carpigiani Group Machine pour la fabrication et la distribution de produits alimentaires liquides, semi-liquides et/ou semi-solides

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