WO2020092315A1 - Enzymatic production of hexoses - Google Patents

Enzymatic production of hexoses Download PDF

Info

Publication number
WO2020092315A1
WO2020092315A1 PCT/US2019/058483 US2019058483W WO2020092315A1 WO 2020092315 A1 WO2020092315 A1 WO 2020092315A1 US 2019058483 W US2019058483 W US 2019058483W WO 2020092315 A1 WO2020092315 A1 WO 2020092315A1
Authority
WO
WIPO (PCT)
Prior art keywords
phosphate
seq
converting
pgm
catalyzed
Prior art date
Application number
PCT/US2019/058483
Other languages
French (fr)
Inventor
Daniel Joseph WICHELECKI
Original Assignee
Bonumose Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bonumose Llc filed Critical Bonumose Llc
Priority to AU2019373147A priority Critical patent/AU2019373147A1/en
Priority to BR112021008314-6A priority patent/BR112021008314A2/en
Priority to MX2021004525A priority patent/MX2021004525A/en
Priority to EP19878605.5A priority patent/EP3874032A4/en
Priority to KR1020217016119A priority patent/KR20210096104A/en
Priority to CN201980086138.9A priority patent/CN113383072A/en
Priority to US17/287,240 priority patent/US20210381014A1/en
Priority to CA3118101A priority patent/CA3118101A1/en
Priority to JP2021523430A priority patent/JP2022512857A/en
Publication of WO2020092315A1 publication Critical patent/WO2020092315A1/en
Priority to ZA2021/02826A priority patent/ZA202102826B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01001—Phosphorylase (2.4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01025—4-Alpha-glucanotransferase (2.4.1.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)

Definitions

  • the invention relates to methods for the preparation of hexose monosaccharides. More specifically, the invention provides improved methods of preparation of an intermediate, glucose 6- phosphate (G6P), using enzymes with higher activities than those previously reported.
  • G6P glucose 6- phosphate
  • Hexoses are monosaccharides with six carbon atoms. Hexoses can be classified by functional group, with aldohexoses having an aldehyde at position 1, and ketohexoses having that ketone at position 2.
  • Aldohexoses (or aldoses) include allose, altrose, glucose, gulose, galactose, idose, talose, and mannose.
  • Ketohexoses (or ketoses) include psicose (allulose), fructose, tagatose, and sorbose.
  • Inositol is a hexose with no aldehyde or ketose group, and is characterized as a carbocyclic sugar.
  • the inventions described herein generally relate to improved processes for preparing a hexose by enzymatic conversion from various saccharide starting materials.
  • the saccharides may be chosen from starch or starch derivatives, cellulose or cellulose derivatives, or sucrose.
  • the enzymes used for the process steps, as indicated have improved activities over enzymes previously disclosed for the preparation of hexoses.
  • the improvement includes at least one of: a) a step of converting glucose 1- phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphoryiase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of transglycosylating a starch derivative, catalyzed by a 4- alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one
  • the improvement includes a step of converting G1P to glucose 6- phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • G6P glucose 6- phosphate
  • PGM phosphogl ucom utase
  • the improvement includes at least one of a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose phosphoryiase, wherein the sucrose phosphoryiase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose.
  • Some improved processes of the invention further include a step of dephosphorylating the hexose-phosphate using a hexose phosphate phosphatase.
  • the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one reaction vessels. In some improved process of the invention, the process steps are conducted ATP-free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction.
  • the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37’C to about 85’C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours. In some improved process of the invention, the process steps are conducted as a continuous reaction.
  • FIG. 1 is a schematic diagram showing an enzymatic pathway converting sucrose to G6P.
  • the following abbreviations are used: SP, sucrose phosphate; and PGM, phosphoglucomutase.
  • SP sucrose phosphate
  • PGM phosphoglucomutase.
  • the improved processes of the invention contain one or more of the following improvements: SP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25, and PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • FIG. 2 is a schematic diagram showing an enzymatic pathway converting a starch derivative, maltodextrin, to G6P.
  • I A isoamylase
  • PA pullulanase
  • aGP alpha- glucan phosphorylase or starch phosphorylase
  • 4GT 4-glucan transferase
  • PGM PGM
  • FIG. 3 is a schematic diagram showing an enzymatic pathway converting cellulose to G6P. The following abbreviations are used: CDP, cellodextrin phosphorylase; and PGM, phosphoglucomutase.
  • PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • FIG. 4 is a schematic diagram showing an enzymatic pathway converting G6P to allose.
  • PGI phosphoglucoisomerase
  • P6PE psicose 6-phosphate 3-epimerase
  • A6PI allose 6-phosphate isomerase
  • A6PP allose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 5 is a schematic diagram showing an enzymatic pathway converting G6P to mannose.
  • PGI phosphoglucoisomerase
  • PMI phosphomannose isomerase
  • M6PP mannose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 6 is a schematic diagram showing an enzymatic pathway converting G6P to galactose.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate isomerase
  • Gal6PI galactose 6-phosphate isomerase
  • Gal6PP galactose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 7 is a schematic diagram showing an enzymatic pathway converting G6P to fructose.
  • PGI phosphoglucoisomerase
  • F6PP fructose 6-phosphate
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 8 is a schematic diagram showing an enzymatic pathway converting G6P to altrose.
  • the following abbreviations are used: PGI, phosphoglucoisomerase; P6PE, psicose 6-phosphate epimerase; Alt6PI, altrose 6-phosphate isomerase; Alt6PP, altrose 6-phosphate phosphatase.
  • PGI phosphoglucoisomerase
  • P6PE psicose 6-phosphate epimerase
  • Alt6PI altrose 6-phosphate isomerase
  • Alt6PP altrose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 9 is a schematic diagram showing an enzymatic pathway converting G6P to talose.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate epimerase
  • Tal6PI talose 6-phosphate isomerase
  • Tal6PP talose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 10 is a schematic diagram showing an enzymatic pathway converting G6P to sorbose.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate epimerase
  • S6PE sorbose 6-phosphate epimerase
  • S6PP sorbose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 11 is a schematic diagram showing an enzymatic pathway converting G6P to gulose.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate epimerase
  • S6PE sorbose 6-phosphate epimerase
  • Gul6PI gulose 6-phosphate isomerase
  • Gul6PP gulose 6- phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 12 is a schematic diagram showing an enzymatic pathway converting G6P to idose.
  • the following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; S6PE, sorbose 6-phosphate epimerase; I6PI, idose 6-phosphate isomerase; I6PP, idose 6-phosphate phosphatase.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate epimerase
  • S6PE sorbose 6-phosphate epimerase
  • I6PI idose 6-phosphate isomerase
  • I6PP idose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 13 is a schematic diagram showing an enzymatic pathway converting G6P to tagatose.
  • the following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; T6PP, tagatose 6-phosphate phosphatase.
  • PGI phosphoglucoisomerase
  • F6PE fructose 6-phosphate epimerase
  • T6PP tagatose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 14 a schematic diagram showing an enzymatic pathway converting G6P to inositol.
  • the following abbreviations are used: IPS, inositol -phosphate synthase; IMP, inositol monophosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or 3.
  • FIG. 15 is a schematic diagram illustrating an enzymatic pathway converting G6P to psicose (allulose).
  • PGI phosphoglucoisomerase
  • P6PE psicose 6- phosphate epimerase
  • P6PP psicose 6-phosphate phosphatase.
  • this pathway is combined with the pathway of FIG. 1, 2, or
  • FIG. 16 shows the Reaction Gibbs Energy between intermediates based on formation Gibbs energy for the conversion of glucose 1-phosphate to another hexose.
  • FIG. 17 shows the conversion of maltodextrin to G6P via HPLC chromatogram.
  • (Dashed line) 0 hour chromatogram;
  • (dotted line) 30 min process with previously disclosed aGP (Uniprot ID G4FEH8) and PGM (Uniprot ID Q68BJ6);
  • (solid line) 30 min “improved” process with higher activity aGP (Uniprot ID D1B926) and higher activity PGM (Uniprot ID A0A150LLZ1).
  • FIG. 18 shows chromatograms of sucrose phosphorylase activities.
  • the top chromatogram shows reactions with Uniprot ID D9TT09 (reference SP) compared to the higher activity SP, Uniprot ID F6BJS0.
  • the 0 hour reaction sucrose to fructose
  • 2 hour reactions using identical amounts of SP and other enzymes are shown for both reference (solid line) and higher activity SP (dotted line).
  • the higher activity SP creates approximately 150% the amount of fructose as the reference SP.
  • the bottom chromatogram compares Uniprot ID D9TT09 (reference SP) to the higher activity Uniprot ID F6BJS0.
  • the 0 hour reaction sucrose to fructose
  • 6 hour reactions using identical amounts of SP and other enzymes are shown for both reference SP (solid line) and higher activity SP (dotted line).
  • the higher activity SP creates approximately 130% the amount of fructose as the reference SP.
  • FIG. 19 is a chromatogram of reactions with Uniprot ID A0A150LLZ1 compared to even higher activity PGMs.
  • the chromatogram shows the level of fructose made from glucose 1-phosphate after incubation with limiting PGM, excess phosphoglucoisomerase, and excess fructose 6-phosphate phosphatase. It is clearly seen that the use of all other PGMs, in equal amounts, results in improved fructose production and therefore enhanced PGM activity since it is the limiting enzyme of the enzymatic process.
  • Uniprot ID A0A0P6YKY9 solid line
  • Uniprot ID E8N4Y6 dashed line
  • Uniprot ID R7RR04 dotted line
  • Uniprot ID A0A023DI95 line of circles
  • UniParc ID UPI0001D17AE3 line of triangles
  • Uniprot ID A0A150LLZ1 line of squares.
  • the inventions described herein provide enzymatic pathways or processes for synthesizing hexoses with a high product yield, while greatly decreasing the product separation costs and hexose production costs.
  • the enyzmatic processes described herein generally relate to improved processes for preparing hexose monosaccharides from saccharides by enzymatic conversion. Artificial (non-natural) ATP-free enzymatic pathways are provided to convert saccharides to a hexose using cell-free enzyme cocktails.
  • the enzymatic processes of the invention involve cell-free preparation of hexoses, have relatively high reaction rates due to the elimination of the cell membrane, which often slows down the transport of substrate/product into and out of the cell.
  • the processes also have a final product free of nutrient-rich fermentation media/cellular metabolites.
  • the saccharides may be chosen from starch or starch derivatives, cellulose or cellulose derivatives, or sucrose.
  • the enzymes used for the process steps, as indicated have improved activities over enzymes previously disclosed for the preparation of hexoses.
  • the invention relates to improved processes for converting starch and its derivatives to a hexose using at least one of an aGP, PGM and 4GT with higher activities instead of aGPs, PGMs and 4GT previously disclosed.
  • aGP Alpha-glucan phosphorylase
  • PGM Phosphoglucomutase
  • Thermococcus kodakaraensis Uniprot ID Q68BJ6
  • 4-alpha- glucanoltransferase from Thermococcus litoralis
  • improvement includes at least one of: a) a step of converting glucose 1-phosphate (G1P) to glucose 6- phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphorylase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of transglycosylating a starch derivative, catalyzed by a 4-alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • G1P glucose 1-phosphate
  • G6P glucose 6- phosphate
  • PGM phosphoglucomutase
  • the process includes: a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphorylase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of
  • transglycosylating a starch derivative catalyzed by a 4-alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs:
  • the invention relates to improved processes for converting cellulose and its derivatives to a hexose using a PGM with higher activity instead of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6).
  • the improvement includes a step of converting G1P to glucose 6-phosphate (G6P), catalyzed by a PGM, wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • the invention relates to improved processes for converting sucrose to a hexose using at least one of a SP with a higher activity instead of the previously disclosed SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09) and a PGM with a higher activity instead of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6).
  • the improvement includes at least one of a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • the process includes a) a step of converting glucose 1- phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose
  • sucrose phosphorylase wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose.
  • Some improved processes of the invention further include a step of dephosphorylating the hexose-phosphate using a hexose phosphate phosphatase,
  • Some improved processes according to the invention for the enzymatic production of a hexose from starch or a starch derivative, cellulose or a cellulose derivative, or sucrose include a a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8. More preferably, the PGM comprises the amino acid sequence of SEQ ID NO: 8.
  • Phosphoglucomutase (EC S.4.2.2) catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate.
  • PGM Phosphoglucomutase
  • the reaction proceeds in the direction of G6P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
  • the PGM has a higher activity relative to the previously described PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6), with the amino acid sequence as listed in SEQ ID NO: 1.
  • PGMs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 100%, at least 200%, at least 500%, at least 1000%, at least 2000%, at least 2500%, at least 3000%, at least 3500%, at least 4000%, at least 4500%, at least 5000%, or at least 5500% over the activity of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6).
  • PGMs for use in processes of the invention have improved activity over PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6): PGM from
  • Geobacillus stearothermophilus NUB3621 (Uniprot ID A0A023CRS6) has enzymatic activity improved by approximately 700%; PGM from Caldibacillus debilis (Uniprot ID A0A150LLZ1) has enzymatic activity improved by approximately 1900%; PGM from Geobacillus thermoglucosidasius (UniParc ID
  • UPI0001D17AE3 has enzymatic activity improved by approximately 2100%; PGM from Parageobacillus caldoxylosilyticus NBRC 107762 (Uniprot ID A0A023DI95) has enzymatic activity improved by approximately 1980%; PGM from Thermobrachium celere DSM 8682 (Uniprot ID R7RR04) has enzymatic activity improved by approximately 5100%; PGM from Anaerolinea thermophila (Uniprot ID E8N4Y6) has enzymatic activity improved by approximately 5800%; and PGM from Thermanaerothrix daxensis (Uniprot ID A0A0P6YKY9) has enzymatic activity improved by approximately 6500%.
  • PGMs for use in the improved process of the invention include but are not limited to the following proteins: PGM from Geobacillus stearothermophilus NUB3621 (Uniprot ID
  • A0A023CRS6 with the amino acid sequence as listed in SEQ ID NO: 2; PGM from Caldibacillus debilis (Uniprot ID A0A150LLZ1) with the amino acid sequence as listed in SEQ ID NO: 3; PGM from Geobacillus thermoglucosidasius (UniParc ID UPI0001D17AE3) with the amino acid sequence as listed in SEQ ID NO: 4; PGM from Parageobacillus caldoxylosilyticus NBRC 107762 (Uniprot ID A0A023DI95) with the amino acid sequence as listed in SEQ ID NO: 5; PGM from Thermobrachium celere DSM 8682 (Uniprot ID R7RR04) with the amino acid sequence as listed in SEQ ID NO: 6; PGM from Anaerolinea thermophila (Uniprot ID E8N4Y6) with the amino acid sequence as listed in SEQ ID NO: 7; and PGM from
  • Thermanaerothrix daxensis (Uniprot ID A0A0P6YKY9) with the amino acid sequence as listed in SEQ ID NO: 8, and PGMs comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to any one of SEQ ID NOs: 2-8.
  • PGMs for use in the improved processes of the invention generally are part of the Alpha-D- phosphohexomutase superfamily (IPR005841) and contain four domains that form the active site of the enzyme.
  • the first three domains consist of an ab core and the forth domain contains a TATA box-binding protein-like fold (Mehra-Chaudhary et al. Proteins 79(4): 1215-29, 2011).
  • the first domain contributes a conserved serine residue for phosphoryl transfer to the active site (Serl47 of SEQ ID NO: 3).
  • the second domain contributes conserved Mg2+ binding residues to the active site (Asp306, Asp308, and Asp310 of SEQ ID NO: 3).
  • the third domain contributes conserved residues for substrate specificity to the active site (Glu406 and Ser408 of SEQ ID NO: 3).
  • the forth domain contributes conserved residues for phosphate binding to the active site (Arg538 of SEQ ID NO: 3).
  • a positively charged residue (Lys/Arg) is conserved with a role in catalysis at Lys420 of SEQ ID No: 3.
  • Some improved processes of the invention for the production of hexose from starch or a starch derivative according to the invention include the step of converting the starch derivative to G1P catalyzed by aGP, wherein the aGP comprises an amino acid sequence having at least 90% amino acid sequence identity with any one of SEQ ID NOs: 10-13.
  • the aGP comprises the amino acid sequence of any one of SEQ ID NOs: 10-13.
  • the aGP comprises the amino acid sequence of SEQ ID NO: 11.
  • Alpha-glucan phosphorylase or starch phosphorylase (aGP) (EC 2.4.1.1) phosphorolytically cleaves maltooligosaccharides to yield G1P.
  • Starch phosphorylase also catalyzes the reverse reaction, that is, the transfer of glucosyl units from G1P to the nonreducing end of alpha-1, 4-D-glucan chains with the release of phosphate.
  • the degree of polymerization of the oligosaccharide chains is 4 or more.
  • the reaction proceeds in the direction of G1P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
  • the aGP has a higher activity relative to the previously described alpha-glucan phosphorylase (aGP) from Thermotoga maritima fUniprot ID
  • aGPs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 50%, at least 100%, at least 150%, or at least 200%, over the activity of aGP from Thermotoga maritima fUniprot ID G4FEH8).
  • aGP from Thermus thermophilus (Uniprot ID Q5SJ42) has enzymatic activity improved by approximately 71% over aGP from Thermotoga maritima fUniprot ID G4FEH8); aGP from Thermus sp.
  • CCB_US3_UF1 (Uniprot ID G8NCC0) has enzymatic activity improved by approximately 186% over aGP from Thermotoga maritima fUniprot ID G4FEH8); aGP from
  • Thermoanaerobacterpseudethanolicus strain ATCC 33223 (Uniprot ID B0K7V8) has enzymatic activity improved by approximately 128% over aGP from Thermotoga maritima fUniprot ID G4FEH8); and aGP from Thermanaerovibrio acidaminovorans strain ATCC 49978 (Uniprot ID D1B926) has enzymatic activity improved by approximately 111% over aGP from Thermotoga maritima fUniprot ID G4FEH8).
  • CCB_US3_UF1 (Uniprot ID G8NCC0), with the amino acid sequence as listed in SEQ ID NO: 11; aGP from Thermoanaerobacter pseudethanolicus strain ATCC 33223 (Uniprot ID B0K7V8), with the amino acid sequence as listed in SEQ ID NO: 12; aGP from Thermanaerovibrio acidaminovorans strain ATCC 49978 (Uniprot ID D1B926), with the amino acid sequence as listed in SEQ ID NO: 13; and aGPs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, or SEQ ID NO: 13.
  • Alpha-glucan phosphorylases for use in the improved processes of the invention generally are part of the Glycosyl transferase, family 35 (IPR000811) and contain the 'alpha glucan phosphoryiase' domain (IPR011834).
  • Some aGP have conserved residues for PLP binding, PLP stabilization, and phosphate binding.
  • LysSSS is conserved for PLP binding
  • Arg484 and ThrSSl are conserved for PLP stabilization
  • GlyllO, Arg485, and Lys490 are conserved for phosphate binding (conserved residues cited from Watson et al. The EM BO Journal Vol.16 No.l pp. 1-14, 1997).
  • the improvement includes a step of transglycosylating the starch derivative using a 4- alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identitiy with any one of SEQ ID Nos: 15-17.
  • the 4GT comprises the amino acid sequence of any one of SEQ ID NOs: 15-17.
  • the aGP comprises the amino acid sequence of SEQ ID NO: 17.
  • maltooligosaccharides yielding maltooligosaccharides of various lengths and glucose.
  • Maltose and glucose can be used as acceptors in the transfer reaction.
  • Some processes of the invention for producing a hexose include an energetically favorable dephosphorylation step converting the hexose phosphate to the hexose.
  • the phosphate is recycled and used upstream in the process, for example, in the reaction catalyzed by aGP converting amylodextrin to G1P.
  • aGP does not react with high activity toward amylodextrins with degrees of polymerization (DP) less than 4.
  • DP degrees of polymerization
  • 4GT is added to the reaction to improve the overall yield.
  • 4GT transglycosylates amylodextrin such that DPs smaller than 4, e.g., maltotriose, are converted into longer chain amylodextrins that can be degraded by aGP. Without 4GT, maltotriose would build up as an end product of the process rather than being recycled back into longer chain amylodextrins that can participate in the process again to enhance yields. 4GT increases yields when amylodextrin is sufficiently degraded by aGP to produce significant amounts of maltotriose. This occurs due to the energetically favorable dephosphorylation step and phosphate recycling.
  • the 4GT has a higher activity relative to the previously described 4-alpha-glucan transferase (4GT) from Thermococcus litoralis (Uniprot ID 032462), with the amino acid sequence as recited in SEQ ID NO:14.
  • 4GT used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 50%, at least 100%, at least 150%, or at least 200%, over the activity of 4GT from Thermococcus litoralis (Uniprot ID 032462).
  • 4GT from Oceanithermus profundus DSM 14977 (Uniprot ID E4U8S9) has enzymatic activity improved by approximately 128% over 4GT from Thermococcus litoralis (Uniprot ID 032462); 4GT from Meiothermus silvanus strain ATCC 700542 (Uniprot ID D7BF07) has enzymatic activity improved by approximately 212% over 4GT from Thermococcus litoralis (Uniprot ID 032462); and 4GT from Anaerolinea thermophila strain DSM 14523 (Uniprot ID E8MXP8) has enzymatic activity improved by approximately 184% over 4GT from Thermococcus litoralis (Uniprot ID 032462).
  • Examples of 4GTs for use in the improved process of the invention include but are not limited to the following proteins: 4GT from Oceanithermus profundus DSM 14977 (Uniprot ID E4U8S9), with the amino acid sequence as listed in SEQ ID NO: 15; 4GT from Meiothermus silvanus strain ATCC 700542 (Uniprot ID D7BF07), with the amino acid sequence as listed in SEQ ID NO: 16; 4GT from Anaerolinea thermophila strain DSM 14523 (Uniprot ID E8MXP8), with the amino acid sequence as listed in SEQ ID NO: 17; and 4GTs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to SEQ ID NO: 15, SEQ ID NO: 16, or SEQ ID NO: 17.
  • 4-glucan transferases for use in the improved processes of the invention geneally are part of the glycoside transferase superfamily (IPR017853), more specifically the glycoside hydrolase, family 77 (IPR003385).
  • 4-glucan transferases contain an ⁇ /a)s-barrel catalytic domain where the active site is at the c-terminal end of the barrel b-strands. The active site contains a conserved catalytic tryad.
  • SEQ ID NO:17 these conserved residues correspond with Asp298 (nucleophile), Glu345 (proton donor), and Asp398 (transition state stabilizer). 4GTs for use in the improved processes of the invention also contain conserved residues associated with substrate binding. For example, in SEQ ID NO: 17, these conserved residues correspond with Tyr60, Asp218, Arg296, and His397. See Przylas et al. Journal of Molecular Biology 296(3): 873-886, 2000.
  • the improvement includes a step of converting sucrose to glucose 1-phosphate (G1P) catalyzed by a sucrose phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identitiy with any one of SEQ ID NOs: 19-25.
  • the SP comprises the amino acid sequence of any one of SEQ ID NOs: 19-25. More preferably, the SP comprises the amino acid sequence of SEQ ID NO: 21.
  • Sucrose phosphorylase (EC:2.4.1.7), catalyzes the conversion of sucrose and inorganic phosphate to fructose and G1P.
  • the reaction proceeds in the direction of G1P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
  • the SP has a higher activity relative to the previously described SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), with the amino acid sequence as listed in SEQ ID NO: 18.
  • SPs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 100% or at least 200%, over the activity of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09).
  • SPs used in the processes of the invention show an improved maximum yield of hexose, in particular fructose.
  • SP from Thermoanaerobacterium sp. PSU-2 (Uniprot ID A0A1X2FWC2) has an enzymatic activity improved by 11% over the activity of of SP from
  • Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 9% higher.
  • SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID L0IL15) has an enzymatic activity improved by 50% over the activity of of SP from Thermoanaerobacterium
  • thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 1% higher.
  • SP from Thermoanaerobacterium xylanolyticum (Uniprot ID F6BJS0) has an enzymatic activity improved by 49% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 32% higher.
  • ZCTH02-B1 (Uniprot ID A0A1Y3Q6Q6) has an enzymatic activity improved by 50% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 3% higher.
  • SP from Bifidobacterium adolescentis (Uniprot ID Q84HQ2) has an enzymatic activity improved by 31% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 37% higher.
  • SP from Paenibacillus thermophilus (Uniprot ID A0A388NK91) has an enzymatic activity improved by 22% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 19% higher.
  • SP from Tepidibacillus decaturensis (Uniprot ID A0A135L6L9) has an enzymatic activity improved by 44% over the activity of of SP from Thermoanaerobacterium
  • thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 9% higher.
  • the increase in maximum yield is significant in a commercial process and comments on the maximum achievable yield of fructose by each SP.
  • contributing factors include product inhibition of sucrose phosphorylase by fructose, the rate of the reverse reaction (G1P + fructose O sucrose + Pi), and more broadly the equilibrium between the formation of fructose and the degradation of fructose at late stages of the reaction.
  • SPs for use in the improved process of the invention include but are not limited to the following proteins: SP from Thermoanaerobacterium sp. PSU-2 (Uniprot ID A0A1X2FWC2), with the amino acid sequence as listed in SEQ ID NO: 19; SP from Thermoanaerobacterium
  • thermosaccharolyticum (Uniprot ID L0IL15), with the amino acid sequence as listed in SEQ ID NO: 20; SP from Thermoanaerobacterium xylanolyticum (Uniprot ID F6BJS0), with the amino acid sequence as listed in SEQ ID NO: 21; SP from Thermobacillus sp.
  • ZCTH02-B1 (Uniprot ID A0A1Y3Q6Q6), with the amino acid sequence as listed in SEQ ID NO: 22; SP from Bifidobacterium adolescentis (Uniprot ID Q84HQ2), with the amino acid sequence as listed in SEQ ID NO: 23; SP from Paenibacillus thermophilus (Uniprot ID A0A388NK91), with the amino acid sequence as listed in SEQ ID NO: 24; SP from Tepidibacillus decaturensis (Uniprot ID A0A135L6L9), with the amino acid sequence as listed in SEQ ID NO: 25; and SPs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to any one of SEQ ID Nos: 19-25.
  • SPs for use in the improved processes of the invention generally are part of the Glycoside hydrolase superfamily (IPR017853), more specifically the sucrose phosphorylase family (IPR022527), and contain the Glycosyl hydrolase, family 13, catalytic domain (IPR006047).
  • the glycosyl hydrolase domain consists of a ⁇ /a) 8 -barrel and is the catalystic domain.
  • sucrose phosphorylases consist of four domains, the N-terminal domain, the glycosyl hydrolyase domain, the B domain (formed from a large loop in the glycosyl domain), and the C-terminal domain (Sprogpe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK (February 2004). "Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis". Biochemistry. 43 (5): 1156-62. doi:10.1021/bi0356395. PMID
  • Leuconostoc mesenteroides (Uniprot Q59495), conservation of the catalytic residues Aspl96 (Schwarz A, Nidetzky B (July 2006). "Asp-196-->Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate". FEBS Letters. 580 (16): 3905-10. doi:10.1016/j.febslet.2006.06.020. PMID 16797542.), Glu237 (Schwarz A, Brecker L, Nidetzky B (May 2007). "Acid-base catalysis in Leuconostoc
  • Starch is the most widely used energy storage compound in nature and is mostly stored in plant seeds. Natural starch contains linear amylose and branched amylopectin. Examples of starch derivatives include amylose, amylopectin, soluble starch, amylodextrin, maltodextrin, maltose, fructose, and glucose.
  • the derivatives of starch can be prepared by enzymatic hydrolysis of starch or by acid hydrolysis of starch. Specifically, the enzymatic hydrolysis of starch can be catalyzed or enhanced by isoamylase (IA, EC.
  • IA isoamylase
  • Isoamylase and pullulanase cleave alpha-1, 6-glycosidic bonds, which allows for more complete degradation of starch by alpha-glucan phosphorylase.
  • Alpha-amylase cleaves alpha-1, 4-glycosidic bonds, therefore alpha-amylase is used to degrade starch into fragments for quicker conversion to a hexose and enhanced solubility.
  • Cellulose is the most abundant bio resource and is the primary component of plant cell walls.
  • Non-food lignocellulosic biomass contains cellulose, hemicellulose, and lignin as well as other minor components.
  • Pure cellulose including Avicel (microcrystalline cellulose), regenerated amorphous cellulose, bacterial cellulose, filter paper, and so on, can be prepared via a series of treatments.
  • the partially hydrolyzed cellulosic substrates include water-insoluble cellodextrins whose degree of polymerization is more than 7, water-soluble cellodextrins with degree of polymerization of 3-6, cellobiose, glucose, and fructose.
  • cellulose derivatives include pretreated biomass, regenerated amorphous cellulose, cellodextrin, cellobiose, fructose, and glucose. Furthermore, derivatives of cellulose can be prepared by enzymatic hydrolysis of cellulose catalyzed by cellulase mixtures, by acids, or by pretreatment of biomass. In some processes of the invention G1P is generated from cellulose by cellulose phosphorylase. In some processes, G1P is generated from cellodextrin and cellobiose and free phosphate catalyzed by cellodextrin phosphorylase (CDP) and cellobiose
  • CDP cellodextrin phosphorylase
  • CBP phosphorylase
  • the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose.
  • Hexoses are monosaccharides with six carbon atoms. Hexoses can be classified by functional group, with
  • aldohexoses having an aldehyde at position 1, and ketohexoses having that ketone at position 2.
  • Aldohexoses include allose, altrose, glucose, gulose, galactose, idose, talose, and mannose.
  • Ketohexoses include psicose (allulose), fructose, tagatose, and sorbose. Inositol has no aldehyde nor ketose group and is characterized as a carbocyclic hexose.
  • Improved processes of the invention can be used to convert starch and its derivatives to hexoses selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose, tagatose, allulose, inositol and idose.
  • hexoses selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose, tagatose, allulose, inositol and idose.
  • an additional step of dephosphorylating a hexose phosphate using a hexose phosphate phosphatase is included.
  • the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one single reaction vessels.
  • the process steps are conducted ATP- free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction.
  • the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37"C to about 85"C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours.
  • the process steps are conducted as a continuous reaction.
  • an additional step of dephosphorylating a hexose phosphate using a hexose phosphate phosphatase is included.
  • the phosphatase used in the processes of the invention is specific for the hexose phosphate.
  • allose 6-phosphate is converted to allose by allose 6-phosphate phosphatase; mannose 6-phosphate is converted to mannose by mannose 6-phosphate phosphatase; galactose 6-phosphate is converted to galactose by galactose 6-phosphate phosphatase; fructose 6-phosphate is converted to fructose by fructose 6-phosphate phosphatase; altrose 6-phosphate is converted to altrose by altrose 6-phosphate phosphatase; talose 6-phosphate is converted to talose by talose 6-phosphate phosphatase; sorbose 6- phosphate is converted to sorbose by sorbose 6-phosphate phosphatase; gulose 6-phosphate is converted to gulose by gulose 6-phosphate phosphatase; tagatose 6-phosphate is converted to tagatose by tagatose 6-phosphate phosphatase; psicose 6-phosphate is converted
  • allose 6-phosphate phosphatase has a higher specific activity on allose 6-phosphate than, for example, sorbose 6-phosphate or talose 6-phosphate.
  • the hexose-phosphate phosphatase has a higher activity for the indicated hexose phosphate compared to other hexose phosphate intermediates in the process.
  • the allose 6-phosphate phosphatase in a process for converting maltodextrin to allose, has a higher activity for allose 6-phosphate compared to the other hexose phosphate intermediates in the process, such as G1P, G6P, F6P, and psicose 6-phospahte. See FIG. 2 and FiG. 4.
  • Sugar alcohols can be made from the various hexose sugars produced by the improved processes of the invention.
  • mannitol and sorbitol could be made and are currently of commercial interest to the medical and food industries.
  • the ketose or aldose product of these improved enzymatic processes can be reduced to the sugar alcohol form using a reducing agent such as hydrogen gas or sodium borohydride.
  • hydrogen gas would be used as the reducing agent as previously described in U.S. Patent Nos. 6,570,043 B2; US 8,816,068 B2; or US 5,466,795 A.
  • the improved process of the invention is directed to the production of allose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to psicose 6-phosphate (P6P) catalyzed by psicose 6-phosphate 3-epimerase (P6PE); converting the P6P to allose 6-phosphate (A6P) catalyzed by allose 6-phosphate isomerase (A6PI); and converting the A6P to allose catalyzed by allose 6-phosphate phosphatase (A6PP).
  • G6P fructose 6- phosphate
  • P6P psicose 6-phosphate
  • P6PE psicose 6-phosphate 3-epimerase
  • A6P allose 6-phosphate
  • A6PI allose 6-phosphate isomerase
  • A6PP allose catalyzed by allose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of mannose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to mannose 6- phosphate (M6P) catalyzed by mannose 6-phosphate isomerase (M6PI) or
  • PGPMI phosphoglucose/phosphomannose isomerase
  • M6PP mannose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of galactose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to tagatose 6- phosphate (T6P) catalyzed by fructose 6-phosphate 4-epimerase (F6PE); converting the T6P to galactose 6-phosphate (Gal6P) catalyzed by galactose 6-phosphate isomerase (Gal6PI); and converting the Gal6P to galactose catalyzed by galactose 6-phosphate phosphatase (Gal6PP).
  • F6P fructose 6- phosphate
  • PKI phosphoglucoisomerase
  • F6PE tagatose 6- phosphate
  • F6PE fructose 6-phosphate 4-epimerase
  • Gal6P galactose 6-phosphate
  • Gal6PP galactose catalyzed by
  • the improved process of the invention is directed to the production of fructose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); and converting the F6P to fructose catalyzed by fructose 6-phosphate phosphatase (F6PP).
  • F6P fructose 6- phosphate
  • PKI phosphoglucoisomerase
  • the improved process of the invention is directed to the production of altrose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to converting the F6P to P6P catalyzed by P6PE; converting the P6P to altrose 6-phosphate (Alt6P) catalyzed by altrose 6- phosphate isomerase (Alt6PI); and converting the Alt6P produced to altrose catalyzed by altrose 6- phosphate phosphatase (Alt6PP).
  • F6P fructose 6- phosphate
  • P6PE phosphoglucoisomerase
  • Alt6P altrose 6-phosphate
  • Alt6PI altrose 6-phosphate isomerase
  • Alt6PP Alt6P produced to altrose catalyzed by altrose 6- phosphate phosphatase
  • the improved process of the invention is directed to the production of talose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to talose 6-phosphate (Tal6P) catalyzed by talose 6-phosphate isomerase (Tal6PI); and converting the Tal6P to talose catalyzed by talose 6-phosphate phosphatase (Tal6PP).
  • F6P fructose 6- phosphate
  • PKI phosphoglucoisomerase
  • T6P talose 6-phosphate
  • Tal6PI talose 6-phosphate isomerase
  • Tal6P converting the Tal6P to talose catalyzed by talose 6-phosphate phosphatase (Tal6PP).
  • the improved process of the invention is directed to the production of sorbose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to sorbose 6-phosphate (S6P) catalyzed by sorbose 6-phosphate epimerase (S6PE); and converting the S6P to sorbose catalyzed by sorbose 6-phosphate phosphatase (S6PP).
  • G6P fructose 6- phosphate
  • PKI phosphoglucoisomerase
  • S6P sorbose 6-phosphate
  • S6PE sorbose 6-phosphate epimerase
  • S6PP sorbose catalyzed by sorbose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of gulose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the S6P to gulose 6-phosphate (Gul6P) catalyzed by gulose 6-phosphate isomerase (Gul6PI); and converting the Gul6P to gulose catalyzed by gulose 6-phosphate phosphatase (Gul6PP).
  • F6P fructose 6- phosphate
  • T6P phosphoglucoisomerase
  • F6PE phosphoglucoisomerase
  • Gul6P gulose 6-phosphate
  • Gul6PI gulose 6-phosphate isomerase
  • Gul6PP gulose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of idose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to sorbose 6-phosphate (S6P) catalyzed by sorbose 6-phosphate epimerase (S6PE); converting the S6P to idose 6-phosphate (I6P) catalyzed by idose 6-phosphate isomerase (I6PI); and converting the I6P to idose catalyzed by idose 6-phosphate phosphatase (I6PP).
  • F6P fructose 6- phosphate
  • T6P catalyzed by F6PE
  • S6P sorbose 6-phosphate
  • S6PE sorbose 6-phosphate epimerase
  • I6PI idose 6-phosphate isomerase
  • I6PP i
  • the improved process of the invention is directed to the production of tagatose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to tagatose 6- phosphate (T6P) catalyzed by fructose 6-phosphate epimerase (F6PE); converting the T6P to tagatose catalyzed by tagatose 6-phosphate phosphatase (T6PP).
  • F6P fructose 6- phosphate
  • PKI phosphoglucoisomerase
  • T6P tagatose 6- phosphate
  • F6PE fructose 6-phosphate epimerase
  • T6PP tagatose catalyzed by tagatose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of psicose.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to psicose 6-phosphate (P6P) catalyzed by psicose 6-phosphate epimerase (P6PE); converting the P6P to psicose catalyzed by psicose 6-phosphate phosphatase (P6PP).
  • G6P fructose 6- phosphate
  • P6P psicose 6-phosphate
  • P6PE psicose 6-phosphate epimerase
  • P6PP psicose catalyzed by psicose 6-phosphate phosphatase
  • the improved process of the invention is directed to the production of inositol.
  • the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • sucrose is the starting material
  • the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
  • Said process further comprises the steps of converting G6P to inositol 3- phosphate (I3P) using inositol phosphate synthase and converting I3P to inositol using inositol monophosphatase.
  • the improved process of the invention is directed to the production of inositol from sucrose.
  • FIG. 1 shows an enzymatic pathway for converting sucrose to the G6P intermediate.
  • the improved processes of the invention contain one or more of the following improvements: SP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25, and PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • the SP comprises the amino acid sequence of any one of SEQ ID NOs: 19-25 and the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8.
  • the SP comprises the amino acid sequence of SEQ ID NO: 21 and the PGM comprises the amino acid sequence of SEQ ID NO: 8.
  • FIG. 2 is a schematic diagram showing an enzymatic pathway converting a starch derivative, maltodextrin, to the G6P intermediate.
  • IA isoamylase
  • PA pullulanase
  • aGP alpha-glucan phosphorylase or starch phosphorylase
  • 4GT 4-glucan transferase
  • PGM phosphoglucomutase
  • PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8
  • aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13
  • 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
  • the PGM comprises the amino acid sequence of any one of SEQ ID NOs: 2-8
  • the aGP comprises an amino acid sequence of any one of SEQ ID NOs: 10-13
  • the 4GT comprises an amino acid sequence of any one of SEQ ID NOs: 15-17.
  • the PGM comprises the amino acid sequence of SEQ ID NO: 8
  • the aGP comprises the amino acid sequence of SEQ ID NO: 11
  • the 4GT comprises the amino acid sequence of SEQ ID NO: 17.
  • FIG. 3 shows an enzymatic pathway for converting cellulose to the G6P intermediate.
  • PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
  • the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8.
  • the PGM comprises the amino acid sequence of SEQ ID NO: 8.ln some improved processes of the invention for the production of a hexose, the one or more of the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one reaction vessels.
  • Phosphate ions produced by dephosphorylation of the hexose phosphate can then be recycled in the process step of converting the starch derivative to G1P, particularly when all process steps are conducted in a single bioreactor or reaction vessel.
  • the ability to recycle phosphate allows for non-stoichiometric amounts of phosphate to be used, which keeps reaction phosphate concentrations low. This affects the overall pathway and the overall rate of the processes, but does not limit the activity of the individual enzymes and allows for overall efficiency of the hexose making processes.
  • the process steps are conducted ATP-free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction.
  • the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37"C to about 85"C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours, or as a continuous process.
  • reaction phosphate concentrations in each of the processes can range from about 0 mM to about 300 mM, from about 0.1 mM to about 150 mM, from about 1 mM to about 50 mM, preferably from about 5 mM to about 50 mM, or more preferably from about 10 mM to about 50 mM.
  • the reaction phosphate concentration in each of the porcesses can be about 0.1 mM, about 0.5 mM, about 1 mM, about 1.5 mM, about 2 mM, about 2.5 mM, about 5 mM, about 6 mM, about 7 mM, about 8 mM, about 9 mM, about 10 mM, about 15 mM, about 20 mM, about 25 mM, about 30 mM, about 35 mM, about 40 mM, about 45 mM, about 50 mM, or about 55 mM.
  • Low phosphate concentration results in decreased production costs due to low total phosphate and thus lowered cost of phosphate removal. It also prevents inhibition of phosphatases or other enzymes in the process by high concentrations of free phosphate and decreases the potential for phosphate pollution.
  • each of the processes disclosed herein can be conducted without added ATP as a source of phosphate, i.e., ATP-free.
  • Each of the processes can also be conducted without having to add NAD(P)(H), I.e., NAD(P)(H)-free.
  • reaction buffer for the processes according to the invention can have a pH ranging from 5.0-8.0. More preferably, the reaction buffer pH can range from about 6.0 to about 7.3.
  • the reaction buffer pH can be 6.0, 6.2, 6.4, 6.6, 6.8, 7.0, 7.2, or 7.3.
  • the reaction buffer can also contain divalent metal cations.
  • the steps are conducted in the presence of a divalent metal cation selected from the group consisting of Mg 2 *, Zn 24 , Ca 24 , Co 24 , Mn 24 , and combinations thereof.
  • suitable salts may be used to introduce the desired metal cation.
  • the reaction temperature at which the process steps are conducted can range from 37-85"C. More preferably, the steps can be conducted at a temperature ranging from about 40"C to about 80"C.
  • the temperature can be, for example, about 40"C, about 45"C, about 50"C, about 55"C, about 60"C, about 65"C, about 70"C, about 75"C, about 80"C, or about 85"C.
  • the reaction temperature is about 40"C, about 45"C, about 50"C, about 55"C, about 60"C, or about 65”C. More preferably the reaction temperature ranges from about about 50"C to about 55"C.
  • the reaction time can be adjusted as necessary, and can range from about 0.5 hours to about 48 hours.
  • the reaction time can be about 1 hour, about 2 hours, about 4 hours, about 8 hours, about 12 hours, about 16 hours, about 18 hours, about 20 hours, about 22 hours, about 24 hours, about 26 hours, about 28 hours, about 30 hours, about 32 hours, about 34 hours, about 36 hours, about 38 hours, about 40 hours, about 42 hours, about 44 hours, about 46 hours, or about 48 hours. More preferably, the reaction time is about 24 hours.
  • the process can also be conducted in one or more reactors as a continuous process, with no set time limit.
  • a solution maltodextrin would be pumped through a bed of immobilized enzyme at such a rate that conversion to tagatose would be complete when the solution leaves one or more columns for downstream processing.
  • 200 g/L of maltodextrin can be pumped through a column packed with immobilized enzymes (maintained at, for example, 50"C) such that when the maltodextrin leaves the column maximum tagatose yield is achieved.
  • This methodology offers greater volumetric productivity over batch methods. This limits the time the product is in contact with the column and reaction conditions, which decreases chances of product degradation (e.g., potential hydroxymethylfurfural formation). It also increases automation in production and therefore reduces operating expenses.
  • the enzymes used in the steps of invention may take the form of soluble, immobilized, assembled, entrapped, or aggregated proteins. These enzymes could be adsorbed on insoluble organic or inorganic supports commonly used to improve functionality, as known in the art. These include polymeric supports such as agarose, methacrylate, polystyrene, or dextran, as well as inorganic supports such as glass, metal, or carbon-based materials. These materials are often produced with large surface- to-volume ratios and specialized surfaces that promote attachment and activity of immobilized enzymes. The enzymes might be affixed to these solid supports through covalent, ionic, or hydrophobic interactions.
  • the enzymes could also be affixed through genetically engineered interactions such as covalent fusion to another protein or peptide sequence with affinity to the solid support, most often a polyhistidine sequence.
  • the enzymes might be affixed either directly to the surface or surface coating, or they might be affixed to other proteins already present on the surface or surface coating.
  • the enzymes can be immobilized all on one carrier, on individual carriers, or a combination of the two (e.g., two enzyme per carrier then mix those carriers). These variations can be mixed evenly or in defined layers to optimize turnover in a continuous process. For example, the beginning of the reactor may have a layer of aGP to ensure a high initial G1P increase. Enzymes may be immobilized all on one carrier, on individual carriers, or in groups. These enzymes may be mixed evenly or in defined layers or zones to optimize turnover.
  • Each of the processes according to the invention can achieve high yields due to the very favorable equilibrium constant for the overall reaction. Theoretically, up to 99% yields can be achieved if the starting material is completely converted to a phosphorylated intermediate.
  • Processes of the invention use low-cost starting materials and reduce production costs by decreasing costs associated with the feedstock and product separation.
  • Starch and its derivatives are less expensive feedstocks than, for example, lactose.
  • glucose and other hexose(s) are separated via chromatography, which leads to higher production costs.
  • the step of hexose dephosphorylation by a phosphatase is an irreversible phosphatase reaction, regardless of the feedstock. Therefore, hexose is produced with a very high yield while effectively minimizing the subsequent product separation costs.
  • Processes according to the invention allow for easy recovery of hexose, and separation costs are minimized.
  • the recovery of the desired hexose is performed without chromatographic separation.
  • the product is instead passed through microfiltration, ion exchange (cation then anion, possible mixed bed for polishing), concentration, crystallization, crystal isolation, and drying. Due to high yields of the hexose, the crystallization step is all that is needed to purify the hexose.
  • hexose prior to crystallization, one can employ nanofiltration to eliminate the risk of enzyme being present in the crystallization process and to remove any unconverted dextrins that may co-crystallize with the hexose or limit the recyclability of the mother liquor (maltodextrin, maltotetraose, maltotriose, maltose, etc.).
  • Improved processes of the invention also include the production of G6P from sucrose, starch or a starch derivative, or cellulose or a cellulose derivative.
  • G6P produced from the processes of the invention may be isolated and purified by techniques known in the art, such as for example, chromatography.
  • ZYM-5052 media including 50 mg L-l kanamycin was used for E. coli cell growth and recombinant protein expression.
  • E. coli BL21 (DE3) strain harboring a protein expression plasmid (pET28a) was incubated in a 1-L Erlenmeyer flask with 100 mL of ZYM-5052 media containing 50 mg L-l kanamycin. Cells were grown at 30"C with rotary shaking at 220 rpm for 16-24 hours. The cells were harvested by
  • Example 1 Improved processes with higher activity PGMs.
  • PGMs phosphogl ucom utases
  • fructose was evaluated using a Hi-Plex H+ column and refractive index detector.
  • the sample was run in 5 mM H2SO4 at 0.6 ml/min for 15.5 min at 65"C.
  • the amount of fructose made in 30 minutes was used to determine the relative efficiencies of each PGM in the enzymatic process.
  • the increase in activity was determined using an average of the increase in fructose peak area and peak height.
  • FIG. 19 is a chromatogram showing activity of various PGMs.
  • the chromatogram shows the level of fructose made from glucose 1-phosphate after incubation with various PGMs, excess phosphoglucoisomerase, and excess fructose 6-phosphate phosphatase.
  • PGM is the limiting enzyme of the cascade.
  • Table 1 shows that PGMs for use in the improved processes of the invention have improved activity relative to the previously disclosed PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6). See International Patent Application Publication WO2017/059278.
  • Example 2 Improved processes with higher activity aGPs.
  • the relative activity of each aGP was measured in the following manner. A 200 m ⁇ reaction of 25 mM sodium phosphate pH 7.2, 5 mM MgCI 2 , 20 g/L maltodextrin DE 4-7, 9 pg of aGP, and 0.1 g/L PGM was prepared and incubated at 50"C. Samples were mixed with 1.5 mM NAD* and 3U/mL glucose 6-phosphate dehydrogenase at various time points. The absorbance at 340 nm at each time point was used to get the rate of reaction. This rate was used to acertain the specific activity of each aGP, which was used for relative activity comparisons.
  • Table 2 shows that aGPs for use in the improved processes of the invention have improved activity relative to the previously disclosed aGP from Thermotoga maritima (Uniprot ID G4FEH8). See International Patent Application Publication WQ2017/059278.
  • Example 3 Improved processes with higher activity 4GTs.
  • the relative activity of each 4GT was measured in the following manner. A 200 pL reaction of 50 mM sodium phosphate pH 7.2, 5 mM MgCI2, 20 g/L maltotriose, and 9 pg of 4GT was prepared and incubated at 50"C. Samples were mixed with 1.5 mM NAD+, 1 mM ATP, 1 U/mL hexokinase, and 1 U/mL glucose 6-phosphate dehydrogenase at various time points. The absorbance at 340 nm at each time point was used to get the rate of reaction. This rate was used to a certain the specific activity of each 4GT, which was used for relative activity comparisons.
  • Table 3 shows that 4GTs for use in the improved processes of the invention have improved activity relative to the previously disclosed 4GT from Thermococcus litoralis (Uniprot ID 032462). See International Patent Application Publication WO2018/169957.
  • Example 4 Improved processes with higher activity SPs.
  • sucrose phosphorylases assumed by homology to Uniprot ID D9TT09 (Verhaeghe et al. The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity. Appl Microbiol Biotechnol. 2014 Aug;98(16):7027-37), were isolated and assayed for converting sucrose to glucose 6-phosphate.
  • the relative efficiency of each SP was measured in the following manner. A reaction of 25 mM sodium phosphate pH 7.2, 5 mM MgCI ⁇ 200 g/L sucrose, 0.15 g/L SP, 0.1 g/L PGM, 0.1 g/L PGI, and 0.3 g/L F6PP was prepared and incubated at 50"C. Samples were taken at 0 hrs 2 hrs, 6 hrs, and 8 hrs. The reactions were stopped via filtration of enzyme with a Vivaspin 2 concentrator (10,000 MWCO). The product, fructose, was evaluated using a Supel Cogel Pb column and refractive index detector.
  • the sample was run in ultrapure water at 0.6 mL/min for 25 min at 80"C.
  • the amount of fructose made at 2 hours is used to determine the relative activities of each SP.
  • the amount of fructose made at 6 hrs shows the differences in maximum achievable yield for each SP.
  • FIG. 18 shows chromatograms of sucrose phosphorylase activities, comparing Uniprot ID D9TT09 (reference SP) to Uniprot ID F6BJS0. At 2 hours, the higher activity SP creates approximately 150% the amount of fructose as the reference SP. The bottom chromatogram compares Uniprot ID D9TT09 (reference SP) to Uniprot ID F6BJS0 in terms of maximum yield.
  • the higher activity SP creates approximately 130% the amount of fructose as the reference SP.
  • the relative yields do not directly correlate with relative activities.
  • contributing factors include product inhibition of sucrose phosphorylase by fructose, the rate of the reverse reaction (G1P + fructose O sucrose + Pi), and more broadly the equilibrium between the formation of fructose and the degradation of fructose at late stages of the reaction.
  • the comparative SP from Thermanaerothrix daxensis (Uniprot ID A0A0N8GPZ6), with amino acid sequence as indicated in SEQ ID NO: 26, showed lower relative activity and lower meaximal yield of fructose compared to the previously disclosed SP.
  • Example 5 Improved enzymatic production of G6P.
  • the reaction was stopped via filtration of enzyme with a Vivaspin 2 concentrator (30,000 MWCO) and analyzed via HPLC (Agilent 1100 series) using an Agilent Hi-Plex H-column and refractive index detector.
  • the sample was run in 5 mM H2SO4 at 0.6 ml/min for 15.5 minutes at 65"C. Results were not quantified as the peaks for the void, maltodextrin, and G6P are too close to quantify any individual component reliably (FIG. 17), but clearly much more G6P is created with the enzymes aGP (Uniprot ID D1B926) and PGM (Uniprot ID A0A150LLZ1).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Disclosed herein are methods of producing hexoses from saccharides by improved enzymatic processes. The improved processes utilize enzymes with higher activities than those previously reported to convert starch or a starch derivative, cellulose or a cellulose derivative, or sucrose to a glucose 6-phosphate (G6P) intermediate.

Description

Enzymatic Production of Hexoses
Cross Reference to Related Applications
[0001] This application claims priority to U.S. Application No. 62/752,061, filed on October 29, 2018, and to U.S. Application No. 62/857,543, filed on June 5, 2019, each herein incorporated by reference.
Field of the Invention
[0002] The invention relates to methods for the preparation of hexose monosaccharides. More specifically, the invention provides improved methods of preparation of an intermediate, glucose 6- phosphate (G6P), using enzymes with higher activities than those previously reported.
Background
[0003] Hexoses are monosaccharides with six carbon atoms. Hexoses can be classified by functional group, with aldohexoses having an aldehyde at position 1, and ketohexoses having that ketone at position 2. Aldohexoses (or aldoses) include allose, altrose, glucose, gulose, galactose, idose, talose, and mannose. Ketohexoses (or ketoses) include psicose (allulose), fructose, tagatose, and sorbose. Inositol is a hexose with no aldehyde or ketose group, and is characterized as a carbocyclic sugar.
[0004] International Patent Application Publication No. WO2018/169957, herein incorporated by reference in its entirety, describes processes for preparing hexoses from saccharides by enzymatic conversion. International Patent Application Publication Nos. WO2017/059278 and W02018/004310 herein incorporated by reference in their entirety, describes processes for preparing tagatose from saccharides by enzymatic conversion. International Patent Application Publication No. WO2018/112139 herein incorporated by reference in its entirety, describes processes for preparing allulose from saccharides by enzymatic conversion. KR Application Publication Number KR20040098757A herein incorporated by reference in its entirety, describes processes for preparing fructose 6-phosphate from saccharides by enzymatic conversion. CN Application Publication Number CN106148425B herein incorporated by reference in its entirety, describes processes for preparing inositol from saccharides by enzymatic conversion. In each of these processes, glucose 6-phosphate (G6P) is an intermediate in the enzymatic pathway.
[0005] Despite developments in enzymatic hexose production with high yields, there is still a need for providing further improved processes of producing hexoses that can, e.g., provide a higher yield with lower amounts of enzymes. There is a strong industrial and commercial interest in decreasing the cost of hexose production, and this decrease involves the use of a reduced amount of enzymes, enzymes with higher activity, and use of combinations of enzymes that are more effective in converting saccharides to the G6P intermediate.
Summary of the Invention
[0006] The inventions described herein generally relate to improved processes for preparing a hexose by enzymatic conversion from various saccharide starting materials. The saccharides may be chosen from starch or starch derivatives, cellulose or cellulose derivatives, or sucrose. In improved processes of the invention, the enzymes used for the process steps, as indicated, have improved activities over enzymes previously disclosed for the preparation of hexoses.
[0007] In some improved processes of the invention for the enzymatic production of a hexose from starch or starch derivative, the improvement includes at least one of: a) a step of converting glucose 1- phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphoryiase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of transglycosylating a starch derivative, catalyzed by a 4- alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
[0008] In some improved processes of the invention for the enzymatic production of a hexose from cellulose or cellulose derivative, the improvement includes a step of converting G1P to glucose 6- phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
[0009] In some improved processes of the invention for the enzymatic production of a hexose from sucrose, the improvement includes at least one of a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphogl ucom utase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose phosphoryiase, wherein the sucrose phosphoryiase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. [0010] In some improved processes of the invention the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose. Some improved processes of the invention further include a step of dephosphorylating the hexose-phosphate using a hexose phosphate phosphatase.
[0011] In some improved processes of the invention, the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one reaction vessels. In some improved process of the invention, the process steps are conducted ATP-free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction. In some improved processes of the invention, the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37’C to about 85’C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours. In some improved process of the invention, the process steps are conducted as a continuous reaction.
Brief Description of the Figures
[0012] FIG. 1 is a schematic diagram showing an enzymatic pathway converting sucrose to G6P. The following abbreviations are used: SP, sucrose phosphate; and PGM, phosphoglucomutase. The improved processes of the invention contain one or more of the following improvements: SP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25, and PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
[0013] FIG. 2 is a schematic diagram showing an enzymatic pathway converting a starch derivative, maltodextrin, to G6P. The following abbreviations are used: I A, isoamylase; PA, pullulanase; aGP, alpha- glucan phosphorylase or starch phosphorylase; 4GT, 4-glucan transferase; and PGM,
phosphoglucomutase. The improved processes of the invention contain one or more of the following improvements: PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. [0014] FIG. 3 is a schematic diagram showing an enzymatic pathway converting cellulose to G6P. The following abbreviations are used: CDP, cellodextrin phosphorylase; and PGM, phosphoglucomutase. In improved processes of the invention, PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
[0015] FIG. 4 is a schematic diagram showing an enzymatic pathway converting G6P to allose. The following abbreviations are used: PGI, phosphoglucoisomerase; P6PE, psicose 6-phosphate 3-epimerase; A6PI, allose 6-phosphate isomerase; A6PP, allose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0016] FIG. 5 is a schematic diagram showing an enzymatic pathway converting G6P to mannose. The following abbreviations are used: PGI, phosphoglucoisomerase; PMI, phosphomannose isomerase; and M6PP, mannose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0017] FIG. 6 is a schematic diagram showing an enzymatic pathway converting G6P to galactose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate isomerase; Gal6PI, galactose 6-phosphate isomerase; Gal6PP, galactose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0018] FIG. 7 is a schematic diagram showing an enzymatic pathway converting G6P to fructose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PP, fructose 6-phosphate
phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0019] FIG. 8 is a schematic diagram showing an enzymatic pathway converting G6P to altrose. The following abbreviations are used: PGI, phosphoglucoisomerase; P6PE, psicose 6-phosphate epimerase; Alt6PI, altrose 6-phosphate isomerase; Alt6PP, altrose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0020] FIG. 9 is a schematic diagram showing an enzymatic pathway converting G6P to talose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; Tal6PI, talose 6-phosphate isomerase; Tal6PP, talose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0021] FIG. 10 is a schematic diagram showing an enzymatic pathway converting G6P to sorbose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; S6PE, sorbose 6-phosphate epimerase; S6PP, sorbose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0022] FIG. 11 is a schematic diagram showing an enzymatic pathway converting G6P to gulose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; S6PE, sorbose 6-phosphate epimerase; Gul6PI, gulose 6-phosphate isomerase; Gul6PP, gulose 6- phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0023] FIG. 12 is a schematic diagram showing an enzymatic pathway converting G6P to idose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; S6PE, sorbose 6-phosphate epimerase; I6PI, idose 6-phosphate isomerase; I6PP, idose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0024] FIG. 13 is a schematic diagram showing an enzymatic pathway converting G6P to tagatose. The following abbreviations are used: PGI, phosphoglucoisomerase; F6PE, fructose 6-phosphate epimerase; T6PP, tagatose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0025] FIG. 14 a schematic diagram showing an enzymatic pathway converting G6P to inositol. The following abbreviations are used: IPS, inositol -phosphate synthase; IMP, inositol monophosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or 3.
[0026] FIG. 15 is a schematic diagram illustrating an enzymatic pathway converting G6P to psicose (allulose). The following abbreviations are used: PGI, phosphoglucoisomerase; P6PE, psicose 6- phosphate epimerase; P6PP, psicose 6-phosphate phosphatase. For an enzymatic pathway for an improved process according to the invention, this pathway is combined with the pathway of FIG. 1, 2, or
3. [0027] FIG. 16 shows the Reaction Gibbs Energy between intermediates based on formation Gibbs energy for the conversion of glucose 1-phosphate to another hexose.
[0028] FIG. 17 shows the conversion of maltodextrin to G6P via HPLC chromatogram. (Dashed line) 0 hour chromatogram; (dotted line) 30 min process with previously disclosed aGP (Uniprot ID G4FEH8) and PGM (Uniprot ID Q68BJ6); and (solid line) 30 min "improved" process with higher activity aGP (Uniprot ID D1B926) and higher activity PGM (Uniprot ID A0A150LLZ1). (1) Void and maltodextrins, (2) G1P and G6P, (3) maltotriose, and (4) maltose.
[0029] FIG. 18 shows chromatograms of sucrose phosphorylase activities. The top chromatogram shows reactions with Uniprot ID D9TT09 (reference SP) compared to the higher activity SP, Uniprot ID F6BJS0. The 0 hour reaction (sucrose to fructose) is in shown as the dashed line and 2 hour reactions using identical amounts of SP and other enzymes are shown for both reference (solid line) and higher activity SP (dotted line). At 2 hours, the higher activity SP creates approximately 150% the amount of fructose as the reference SP. The bottom chromatogram compares Uniprot ID D9TT09 (reference SP) to the higher activity Uniprot ID F6BJS0. The 0 hour reaction (sucrose to fructose) is in is shown as a dashed line and 6 hour reactions using identical amounts of SP and other enzymes are shown for both reference SP (solid line) and higher activity SP (dotted line). At 6 hours (maximum yield for both reactions), the higher activity SP creates approximately 130% the amount of fructose as the reference SP.
[0030] FIG. 19 is a chromatogram of reactions with Uniprot ID A0A150LLZ1 compared to even higher activity PGMs. The chromatogram shows the level of fructose made from glucose 1-phosphate after incubation with limiting PGM, excess phosphoglucoisomerase, and excess fructose 6-phosphate phosphatase. It is clearly seen that the use of all other PGMs, in equal amounts, results in improved fructose production and therefore enhanced PGM activity since it is the limiting enzyme of the enzymatic process. In the figure, Uniprot ID A0A0P6YKY9 = solid line; Uniprot ID E8N4Y6 = dashed line; Uniprot ID R7RR04 = dotted line; Uniprot ID A0A023DI95 = line of circles; UniParc ID UPI0001D17AE3 = line of triangles; and Uniprot ID A0A150LLZ1 = line of squares. The activity of the reference PGM Uniprot ID Q68BJ6, which was much lower (see Table 1), is not shown.
Detailed Description
[0031] The inventions described herein provide enzymatic pathways or processes for synthesizing hexoses with a high product yield, while greatly decreasing the product separation costs and hexose production costs. The enyzmatic processes described herein generally relate to improved processes for preparing hexose monosaccharides from saccharides by enzymatic conversion. Artificial (non-natural) ATP-free enzymatic pathways are provided to convert saccharides to a hexose using cell-free enzyme cocktails. In contrast to cell-based manufacturing methods, the enzymatic processes of the invention involve cell-free preparation of hexoses, have relatively high reaction rates due to the elimination of the cell membrane, which often slows down the transport of substrate/product into and out of the cell. The processes also have a final product free of nutrient-rich fermentation media/cellular metabolites. The saccharides may be chosen from starch or starch derivatives, cellulose or cellulose derivatives, or sucrose. In improved processes of the invention, the enzymes used for the process steps, as indicated, have improved activities over enzymes previously disclosed for the preparation of hexoses.
[001] In one embodiment, the invention relates to improved processes for converting starch and its derivatives to a hexose using at least one of an aGP, PGM and 4GT with higher activities instead of aGPs, PGMs and 4GT previously disclosed. See International Patent Application Publication WO2018/169957, disclosing Alpha-glucan phosphorylase (aGP) from Thermotoga maritima (Uniprot ID G4FEH8);
Phosphoglucomutase (PGM) from Thermococcus kodakaraensis (Uniprot ID Q68BJ6); and 4-alpha- glucanoltransferase from Thermococcus litoralis (Uniprot ID 032462). In some improved processes of the invention for the enzymatic production of a hexose from starch or starch derivative, the
improvement includes at least one of: a) a step of converting glucose 1-phosphate (G1P) to glucose 6- phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphorylase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of transglycosylating a starch derivative, catalyzed by a 4-alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. In an improved process of the invention, the process includes: a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphorylase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and a step of
transglycosylating a starch derivative, catalyzed by a 4-alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs:
15-17.
[0032] In another embodiment, the invention relates to improved processes for converting cellulose and its derivatives to a hexose using a PGM with higher activity instead of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6). In some improved processes of the invention for the enzymatic production of a hexose from cellulose or cellulose derivative, the improvement includes a step of converting G1P to glucose 6-phosphate (G6P), catalyzed by a PGM, wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
[0033] In yet another embodiment, the invention relates to improved processes for converting sucrose to a hexose using at least one of a SP with a higher activity instead of the previously disclosed SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09) and a PGM with a higher activity instead of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6). In some improved processes of the invention for the enzymatic production of a hexose from sucrose, the improvement includes at least one of a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. In an improved process of the invention, the process includes a) a step of converting glucose 1- phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose
phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
[0034] In some improved processes of the invention the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose. Some improved processes of the invention further include a step of dephosphorylating the hexose-phosphate using a hexose phosphate phosphatase,
[0035] Some improved processes according to the invention for the enzymatic production of a hexose from starch or a starch derivative, cellulose or a cellulose derivative, or sucrose, include a a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. Preferably, the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8. More preferably, the PGM comprises the amino acid sequence of SEQ ID NO: 8.
[0036] Phosphoglucomutase (PGM) (EC S.4.2.2) catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate. In an improved process of the invention for the production of hexoses from a saccharide, the reaction proceeds in the direction of G6P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
[0037] In improved processes of the invention, the PGM has a higher activity relative to the previously described PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6), with the amino acid sequence as listed in SEQ ID NO: 1. Preferably, PGMs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 100%, at least 200%, at least 500%, at least 1000%, at least 2000%, at least 2500%, at least 3000%, at least 3500%, at least 4000%, at least 4500%, at least 5000%, or at least 5500% over the activity of PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6).
[0038] For instance, as shown in Example 1, PGMs for use in processes of the invention have improved activity over PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6): PGM from
Geobacillus stearothermophilus NUB3621 (Uniprot ID A0A023CRS6) has enzymatic activity improved by approximately 700%; PGM from Caldibacillus debilis (Uniprot ID A0A150LLZ1) has enzymatic activity improved by approximately 1900%; PGM from Geobacillus thermoglucosidasius (UniParc ID
UPI0001D17AE3) has enzymatic activity improved by approximately 2100%; PGM from Parageobacillus caldoxylosilyticus NBRC 107762 (Uniprot ID A0A023DI95) has enzymatic activity improved by approximately 1980%; PGM from Thermobrachium celere DSM 8682 (Uniprot ID R7RR04) has enzymatic activity improved by approximately 5100%; PGM from Anaerolinea thermophila (Uniprot ID E8N4Y6) has enzymatic activity improved by approximately 5800%; and PGM from Thermanaerothrix daxensis (Uniprot ID A0A0P6YKY9) has enzymatic activity improved by approximately 6500%. The examples below provide protocols to those skilled in the art for determining activity of PGMs as part of an enzymatic process, which involve, for example, incubating the enzyme with its substrate, and then measuring the amounts of reactants and products, or subbsequent downstream products, via HPLC. Measurements of relative activities any two enzymes are performed under identical reaction conditions such as buffer, pH, temperature, etc. [0039] Examples of PGMs for use in the improved process of the invention include but are not limited to the following proteins: PGM from Geobacillus stearothermophilus NUB3621 (Uniprot ID
A0A023CRS6) with the amino acid sequence as listed in SEQ ID NO: 2; PGM from Caldibacillus debilis (Uniprot ID A0A150LLZ1) with the amino acid sequence as listed in SEQ ID NO: 3; PGM from Geobacillus thermoglucosidasius (UniParc ID UPI0001D17AE3) with the amino acid sequence as listed in SEQ ID NO: 4; PGM from Parageobacillus caldoxylosilyticus NBRC 107762 (Uniprot ID A0A023DI95) with the amino acid sequence as listed in SEQ ID NO: 5; PGM from Thermobrachium celere DSM 8682 (Uniprot ID R7RR04) with the amino acid sequence as listed in SEQ ID NO: 6; PGM from Anaerolinea thermophila (Uniprot ID E8N4Y6) with the amino acid sequence as listed in SEQ ID NO: 7; and PGM from
Thermanaerothrix daxensis (Uniprot ID A0A0P6YKY9) with the amino acid sequence as listed in SEQ ID NO: 8, and PGMs comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to any one of SEQ ID NOs: 2-8.
[0040] PGMs for use in the improved processes of the invention generally are part of the Alpha-D- phosphohexomutase superfamily (IPR005841) and contain four domains that form the active site of the enzyme. The first three domains consist of an ab core and the forth domain contains a TATA box-binding protein-like fold (Mehra-Chaudhary et al. Proteins 79(4): 1215-29, 2011). The first domain contributes a conserved serine residue for phosphoryl transfer to the active site (Serl47 of SEQ ID NO: 3). The second domain contributes conserved Mg2+ binding residues to the active site (Asp306, Asp308, and Asp310 of SEQ ID NO: 3). The third domain contributes conserved residues for substrate specificity to the active site (Glu406 and Ser408 of SEQ ID NO: 3). The forth domain contributes conserved residues for phosphate binding to the active site (Arg538 of SEQ ID NO: 3). Additionally, a positively charged residue (Lys/Arg) is conserved with a role in catalysis at Lys420 of SEQ ID No: 3. Conserved residues cited from Lee et al. FEBS J 280(11): 2622-32, 2013 and Levin et al. Protein Engineering, Design and Selection 12(9): 737-746, 1999.
[0041] Some improved processes of the invention for the production of hexose from starch or a starch derivative according to the invention include the step of converting the starch derivative to G1P catalyzed by aGP, wherein the aGP comprises an amino acid sequence having at least 90% amino acid sequence identity with any one of SEQ ID NOs: 10-13. Preferably, the aGP comprises the amino acid sequence of any one of SEQ ID NOs: 10-13. More preferably, the aGP comprises the amino acid sequence of SEQ ID NO: 11. [0042] Alpha-glucan phosphorylase or starch phosphorylase (aGP) (EC 2.4.1.1) phosphorolytically cleaves maltooligosaccharides to yield G1P. Starch phosphorylase also catalyzes the reverse reaction, that is, the transfer of glucosyl units from G1P to the nonreducing end of alpha-1, 4-D-glucan chains with the release of phosphate. Generally, the degree of polymerization of the oligosaccharide chains is 4 or more. In improved process of the invention for the production of hexoses from a starch derivative, the reaction proceeds in the direction of G1P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
[0043] In the improved processes of the invention, the aGP has a higher activity relative to the previously described alpha-glucan phosphorylase (aGP) from Thermotoga maritima fUniprot ID
G4FEH8), with the amino acid sequence as listed in SEQ ID NO: 9. Preferably, aGPs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 50%, at least 100%, at least 150%, or at least 200%, over the activity of aGP from Thermotoga maritima fUniprot ID G4FEH8). For instance, as shown in Example 2, aGP from Thermus thermophilus (Uniprot ID Q5SJ42) has enzymatic activity improved by approximately 71% over aGP from Thermotoga maritima fUniprot ID G4FEH8); aGP from Thermus sp. CCB_US3_UF1 (Uniprot ID G8NCC0) has enzymatic activity improved by approximately 186% over aGP from Thermotoga maritima fUniprot ID G4FEH8); aGP from
Thermoanaerobacterpseudethanolicus strain ATCC 33223 (Uniprot ID B0K7V8) has enzymatic activity improved by approximately 128% over aGP from Thermotoga maritima fUniprot ID G4FEH8); and aGP from Thermanaerovibrio acidaminovorans strain ATCC 49978 (Uniprot ID D1B926) has enzymatic activity improved by approximately 111% over aGP from Thermotoga maritima fUniprot ID G4FEH8). The examples below provide protocols to those skilled in the art for determining activity of aGPs as part of an enzymatic process, which involve, for example, incubating the enzyme with its substrate, and then measuring the amounts of reactants and products or, subsequent downstream products, via spectrophotometric measurements and HPLC. Measurements of relative activities any two enzymes are performed under identical reaction conditions such as buffer, pH, temperature, etc.
[0044] Examples of aGPs for use in the improved process of the invention include but are not limited to the following proteins: aGP from Thermus thermophilus (Uniprot ID Q5SJ42), with the amino acid sequence as listed in SEQ ID NO: 10; aGP from Thermus sp. CCB_US3_UF1 (Uniprot ID G8NCC0), with the amino acid sequence as listed in SEQ ID NO: 11; aGP from Thermoanaerobacter pseudethanolicus strain ATCC 33223 (Uniprot ID B0K7V8), with the amino acid sequence as listed in SEQ ID NO: 12; aGP from Thermanaerovibrio acidaminovorans strain ATCC 49978 (Uniprot ID D1B926), with the amino acid sequence as listed in SEQ ID NO: 13; and aGPs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, or SEQ ID NO: 13.
[0045] Alpha-glucan phosphorylases for use in the improved processes of the invention generally are part of the Glycosyl transferase, family 35 (IPR000811) and contain the 'alpha glucan phosphoryiase' domain (IPR011834). Some aGP have conserved residues for PLP binding, PLP stabilization, and phosphate binding. For example, in Uniprot ID D1B926 (SEQ ID NO: 13) LysSSS is conserved for PLP binding; Arg484 and ThrSSl are conserved for PLP stabilization; and GlyllO, Arg485, and Lys490 are conserved for phosphate binding (conserved residues cited from Watson et al. The EM BO Journal Vol.16 No.l pp. 1-14, 1997).
[0046] In some improved process according to the invention for the production of a hexose from a starch derivative, the improvement includes a step of transglycosylating the starch derivative using a 4- alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identitiy with any one of SEQ ID Nos: 15-17. Preferably, the 4GT comprises the amino acid sequence of any one of SEQ ID NOs: 15-17. More preferably, the aGP comprises the amino acid sequence of SEQ ID NO: 17.
[0047] 4-alpha-glucan transferase (4GT) (EC 2.4.1.25) catalyzes the transglycosylation of
maltooligosaccharides, yielding maltooligosaccharides of various lengths and glucose. Maltose and glucose can be used as acceptors in the transfer reaction.
[0048] Some processes of the invention for producing a hexose include an energetically favorable dephosphorylation step converting the hexose phosphate to the hexose. In such processes, especially when conducted in a single reaction vessel, the phosphate is recycled and used upstream in the process, for example, in the reaction catalyzed by aGP converting amylodextrin to G1P. However, aGP does not react with high activity toward amylodextrins with degrees of polymerization (DP) less than 4. In such processes according to the invention, 4GT is added to the reaction to improve the overall yield. 4GT transglycosylates amylodextrin such that DPs smaller than 4, e.g., maltotriose, are converted into longer chain amylodextrins that can be degraded by aGP. Without 4GT, maltotriose would build up as an end product of the process rather than being recycled back into longer chain amylodextrins that can participate in the process again to enhance yields. 4GT increases yields when amylodextrin is sufficiently degraded by aGP to produce significant amounts of maltotriose. This occurs due to the energetically favorable dephosphorylation step and phosphate recycling.
[0049] In the improved processes of the invention, the 4GT has a higher activity relative to the previously described 4-alpha-glucan transferase (4GT) from Thermococcus litoralis (Uniprot ID 032462), with the amino acid sequence as recited in SEQ ID NO:14. Preferably, 4GT used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 50%, at least 100%, at least 150%, or at least 200%, over the activity of 4GT from Thermococcus litoralis (Uniprot ID 032462). For instance, as shown in Example 3, 4GT from Oceanithermus profundus DSM 14977 (Uniprot ID E4U8S9) has enzymatic activity improved by approximately 128% over 4GT from Thermococcus litoralis (Uniprot ID 032462); 4GT from Meiothermus silvanus strain ATCC 700542 (Uniprot ID D7BF07) has enzymatic activity improved by approximately 212% over 4GT from Thermococcus litoralis (Uniprot ID 032462); and 4GT from Anaerolinea thermophila strain DSM 14523 (Uniprot ID E8MXP8) has enzymatic activity improved by approximately 184% over 4GT from Thermococcus litoralis (Uniprot ID 032462). The examples below provide protocols to those skilled in the art for determining activity of 4GTs as part of an enzymatic process, which involve, for example, incubating the enzyme with its substrate, and then measuring the amounts of glucose via spectrophotometric measurements. Measurements of relative activities any two enzymes are performed under identical reaction conditions such as buffer, pH, temperature, etc.
[0050] Examples of 4GTs for use in the improved process of the invention include but are not limited to the following proteins: 4GT from Oceanithermus profundus DSM 14977 (Uniprot ID E4U8S9), with the amino acid sequence as listed in SEQ ID NO: 15; 4GT from Meiothermus silvanus strain ATCC 700542 (Uniprot ID D7BF07), with the amino acid sequence as listed in SEQ ID NO: 16; 4GT from Anaerolinea thermophila strain DSM 14523 (Uniprot ID E8MXP8), with the amino acid sequence as listed in SEQ ID NO: 17; and 4GTs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to SEQ ID NO: 15, SEQ ID NO: 16, or SEQ ID NO: 17.
[0051] 4-glucan transferases for use in the improved processes of the invention geneally are part of the glycoside transferase superfamily (IPR017853), more specifically the glycoside hydrolase, family 77 (IPR003385). Generally, 4-glucan transferases contain an ^/a)s-barrel catalytic domain where the active site is at the c-terminal end of the barrel b-strands. The active site contains a conserved catalytic tryad.
In SEQ ID NO:17, these conserved residues correspond with Asp298 (nucleophile), Glu345 (proton donor), and Asp398 (transition state stabilizer). 4GTs for use in the improved processes of the invention also contain conserved residues associated with substrate binding. For example, in SEQ ID NO: 17, these conserved residues correspond with Tyr60, Asp218, Arg296, and His397. See Przylas et al. Journal of Molecular Biology 296(3): 873-886, 2000.
[0052] In some improved processes for the enzymatic production of a hexose from sucrose, the improvement includes a step of converting sucrose to glucose 1-phosphate (G1P) catalyzed by a sucrose phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identitiy with any one of SEQ ID NOs: 19-25. Preferably, the SP comprises the amino acid sequence of any one of SEQ ID NOs: 19-25. More preferably, the SP comprises the amino acid sequence of SEQ ID NO: 21.
[0053] Sucrose phosphorylase, (EC:2.4.1.7), catalyzes the conversion of sucrose and inorganic phosphate to fructose and G1P. In improved process of the invention for the production of hexoses from sucrose, the reaction proceeds in the direction of G1P, which is then processed further downstream where the final enzymatic step in the process is an energetically favorable, irreversible step of dephosphorylation of a hexose phosphate.
[0054] In the improved processes of the invention, the SP has a higher activity relative to the previously described SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), with the amino acid sequence as listed in SEQ ID NO: 18. Preferably, SPs used in the processes of the invention have an enzymatic activity improved by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 100% or at least 200%, over the activity of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09). In some embodiments, SPs used in the processes of the invention show an improved maximum yield of hexose, in particular fructose. For instance, several SP with higher activities are shown in Example 4. SP from Thermoanaerobacterium sp. PSU-2 (Uniprot ID A0A1X2FWC2) has an enzymatic activity improved by 11% over the activity of of SP from
Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 9% higher. SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID L0IL15) has an enzymatic activity improved by 50% over the activity of of SP from Thermoanaerobacterium
thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 1% higher. SP from Thermoanaerobacterium xylanolyticum (Uniprot ID F6BJS0) has an enzymatic activity improved by 49% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 32% higher. SP from Thermobacillus sp. ZCTH02-B1 (Uniprot ID A0A1Y3Q6Q6) has an enzymatic activity improved by 50% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 3% higher. SP from Bifidobacterium adolescentis (Uniprot ID Q84HQ2) has an enzymatic activity improved by 31% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 37% higher. SP from Paenibacillus thermophilus (Uniprot ID A0A388NK91) has an enzymatic activity improved by 22% over the activity of of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 19% higher. SP from Tepidibacillus decaturensis (Uniprot ID A0A135L6L9) has an enzymatic activity improved by 44% over the activity of of SP from Thermoanaerobacterium
thermosaccharolyticum (Uniprot ID D9TT09), and a maximum yield of fructose that is 9% higher. The increase in maximum yield is significant in a commercial process and comments on the maximum achievable yield of fructose by each SP. Presumably, contributing factors include product inhibition of sucrose phosphorylase by fructose, the rate of the reverse reaction (G1P + fructose O sucrose + Pi), and more broadly the equilibrium between the formation of fructose and the degradation of fructose at late stages of the reaction.
[0055] The examples below provide protocols to those skilled in the art for determining activity of SPs as part of an enzymatic process, which involve, for example, incubating the enzyme with its substrate, and then measuring the amounts of reactants and products, or subsequent downstream products, via HPLC. Measurements of relative activities any two enzymes are performed under identical reaction conditions such as buffer, pH, temperature, etc.
[0056] Examples of SPs for use in the improved process of the invention include but are not limited to the following proteins: SP from Thermoanaerobacterium sp. PSU-2 (Uniprot ID A0A1X2FWC2), with the amino acid sequence as listed in SEQ ID NO: 19; SP from Thermoanaerobacterium
thermosaccharolyticum (Uniprot ID L0IL15), with the amino acid sequence as listed in SEQ ID NO: 20; SP from Thermoanaerobacterium xylanolyticum (Uniprot ID F6BJS0), with the amino acid sequence as listed in SEQ ID NO: 21; SP from Thermobacillus sp. ZCTH02-B1 (Uniprot ID A0A1Y3Q6Q6), with the amino acid sequence as listed in SEQ ID NO: 22; SP from Bifidobacterium adolescentis (Uniprot ID Q84HQ2), with the amino acid sequence as listed in SEQ ID NO: 23; SP from Paenibacillus thermophilus (Uniprot ID A0A388NK91), with the amino acid sequence as listed in SEQ ID NO: 24; SP from Tepidibacillus decaturensis (Uniprot ID A0A135L6L9), with the amino acid sequence as listed in SEQ ID NO: 25; and SPs having at least 90%, at least 95%, at least 97%, at least 99%, or 100% amino acid sequence identity to any one of SEQ ID Nos: 19-25. [0057] SPs for use in the improved processes of the invention generally are part of the Glycoside hydrolase superfamily (IPR017853), more specifically the sucrose phosphorylase family (IPR022527), and contain the Glycosyl hydrolase, family 13, catalytic domain (IPR006047). The glycosyl hydrolase domain consists of a ^/a)8-barrel and is the catalystic domain. In total sucrose phosphorylases consist of four domains, the N-terminal domain, the glycosyl hydrolyase domain, the B domain (formed from a large loop in the glycosyl domain), and the C-terminal domain (Sprogpe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK (February 2004). "Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis". Biochemistry. 43 (5): 1156-62. doi:10.1021/bi0356395. PMID
14756551.). Through sequence alignments to the well studied sucrose phosphorylase from
Leuconostoc mesenteroides (Uniprot Q59495), conservation of the catalytic residues Aspl96 (Schwarz A, Nidetzky B (July 2006). "Asp-196-->Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate". FEBS Letters. 580 (16): 3905-10. doi:10.1016/j.febslet.2006.06.020. PMID 16797542.), Glu237 (Schwarz A, Brecker L, Nidetzky B (May 2007). "Acid-base catalysis in Leuconostoc
mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237~>Gln mutant enzymes". The Biochemical Journal. 403 (3): 441-9. doi : 10.1042/BJ20070042. PMC 1876375. PMID 17233628.), and Asp 295 (Mueller M, Nidetzky B (April 2007). "The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis". FEBS Letters. 581 (7): 1403-8.
doi:10.1016/j.febslet.2007.02.060. PMID 17350620.) is seen.
[0058] Starch is the most widely used energy storage compound in nature and is mostly stored in plant seeds. Natural starch contains linear amylose and branched amylopectin. Examples of starch derivatives include amylose, amylopectin, soluble starch, amylodextrin, maltodextrin, maltose, fructose, and glucose. The derivatives of starch can be prepared by enzymatic hydrolysis of starch or by acid hydrolysis of starch. Specifically, the enzymatic hydrolysis of starch can be catalyzed or enhanced by isoamylase (IA, EC. 3.2.1.68), which hydrolyzes a-l,6-glucosidic bonds; pullulanase (PA, EC. 3.2.1.41), which hydrolyzes a-l,6-glucosidic bonds; or alpha-amylase (EC 3.2.1.1), which cleaves a-l,4-glucosidic bonds. Corn starch contains many branches that impede aGP action. Isoamylase can be used to de- branch starch, yielding linear amylodextrin. Isoamylase-pretreated starch can result in a higher F6P concentration in the final product. Isoamylase and pullulanase cleave alpha-1, 6-glycosidic bonds, which allows for more complete degradation of starch by alpha-glucan phosphorylase. Alpha-amylase cleaves alpha-1, 4-glycosidic bonds, therefore alpha-amylase is used to degrade starch into fragments for quicker conversion to a hexose and enhanced solubility.
[0059] Cellulose is the most abundant bio resource and is the primary component of plant cell walls. Non-food lignocellulosic biomass contains cellulose, hemicellulose, and lignin as well as other minor components. Pure cellulose, including Avicel (microcrystalline cellulose), regenerated amorphous cellulose, bacterial cellulose, filter paper, and so on, can be prepared via a series of treatments. The partially hydrolyzed cellulosic substrates include water-insoluble cellodextrins whose degree of polymerization is more than 7, water-soluble cellodextrins with degree of polymerization of 3-6, cellobiose, glucose, and fructose. Examples of cellulose derivatives include pretreated biomass, regenerated amorphous cellulose, cellodextrin, cellobiose, fructose, and glucose. Furthermore, derivatives of cellulose can be prepared by enzymatic hydrolysis of cellulose catalyzed by cellulase mixtures, by acids, or by pretreatment of biomass. In some processes of the invention G1P is generated from cellulose by cellulose phosphorylase. In some processes, G1P is generated from cellodextrin and cellobiose and free phosphate catalyzed by cellodextrin phosphorylase (CDP) and cellobiose
phosphorylase (CBP).
[0060] In some improved processes of the invention the hexose is selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose. Hexoses are monosaccharides with six carbon atoms. Hexoses can be classified by functional group, with
aldohexoses having an aldehyde at position 1, and ketohexoses having that ketone at position 2.
Aldohexoses (or aldoses) include allose, altrose, glucose, gulose, galactose, idose, talose, and mannose. Ketohexoses (or ketoses) include psicose (allulose), fructose, tagatose, and sorbose. Inositol has no aldehyde nor ketose group and is characterized as a carbocyclic hexose. Improved processes of the invention can be used to convert starch and its derivatives to hexoses selected from allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose, tagatose, allulose, inositol and idose. Hence several of the embodiments of the invention relate to improved processes for the production these individual hexoses.
[0061] In an improved process of the invention for the production of a hexose, an additional step of dephosphorylating a hexose phosphate using a hexose phosphate phosphatase is included. In some improved processes of the invention for the production of hexoses, the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one single reaction vessels. In some improved process, the process steps are conducted ATP- free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction. In some improved processes of the invention, the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37"C to about 85"C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours. In some improved process of the invention, the process steps are conducted as a continuous reaction.
[0062] In some processes of the invention for the preparation of a hexose, an additional step of dephosphorylating a hexose phosphate using a hexose phosphate phosphatase is included. The phosphatase used in the processes of the invention is specific for the hexose phosphate. For example, allose 6-phosphate is converted to allose by allose 6-phosphate phosphatase; mannose 6-phosphate is converted to mannose by mannose 6-phosphate phosphatase; galactose 6-phosphate is converted to galactose by galactose 6-phosphate phosphatase; fructose 6-phosphate is converted to fructose by fructose 6-phosphate phosphatase; altrose 6-phosphate is converted to altrose by altrose 6-phosphate phosphatase; talose 6-phosphate is converted to talose by talose 6-phosphate phosphatase; sorbose 6- phosphate is converted to sorbose by sorbose 6-phosphate phosphatase; gulose 6-phosphate is converted to gulose by gulose 6-phosphate phosphatase; tagatose 6-phosphate is converted to tagatose by tagatose 6-phosphate phosphatase; psicose 6-phosphate is converted to psicose by psicose 6- phosphate phosphatase; inositol 3-phosphate is converted to inositol by inositol monophosphatase; and idose 6-phosphate is converted to idose by idose 6-phosphate phosphatase. As used herein, specific means having a higher specfic activity for the indicated hexose over other hexoses. For instance, allose 6-phosphate phosphatase has a higher specific activity on allose 6-phosphate than, for example, sorbose 6-phosphate or talose 6-phosphate. In processes of the invention, the hexose-phosphate phosphatase has a higher activity for the indicated hexose phosphate compared to other hexose phosphate intermediates in the process. As an illustrative example, in a process for converting maltodextrin to allose, the allose 6-phosphate phosphatase has a higher activity for allose 6-phosphate compared to the other hexose phosphate intermediates in the process, such as G1P, G6P, F6P, and psicose 6-phospahte. See FIG. 2 and FiG. 4.
[0063] Sugar alcohols can be made from the various hexose sugars produced by the improved processes of the invention. For example, mannitol and sorbitol could be made and are currently of commercial interest to the medical and food industries. The ketose or aldose product of these improved enzymatic processes can be reduced to the sugar alcohol form using a reducing agent such as hydrogen gas or sodium borohydride. Preferably, hydrogen gas would be used as the reducing agent as previously described in U.S. Patent Nos. 6,570,043 B2; US 8,816,068 B2; or US 5,466,795 A.
[0064] In one embodiment, the improved process of the invention is directed to the production of allose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to psicose 6-phosphate (P6P) catalyzed by psicose 6-phosphate 3-epimerase (P6PE); converting the P6P to allose 6-phosphate (A6P) catalyzed by allose 6-phosphate isomerase (A6PI); and converting the A6P to allose catalyzed by allose 6-phosphate phosphatase (A6PP).
[0065] In one embodiment, the improved process of the invention is directed to the production of mannose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to mannose 6- phosphate (M6P) catalyzed by mannose 6-phosphate isomerase (M6PI) or
phosphoglucose/phosphomannose isomerase (PGPMI); and converting the M6P to mannose catalyzed by mannose 6-phosphate phosphatase (M6PP).
[0066] In one embodiment, the improved process of the invention is directed to the production of galactose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to tagatose 6- phosphate (T6P) catalyzed by fructose 6-phosphate 4-epimerase (F6PE); converting the T6P to galactose 6-phosphate (Gal6P) catalyzed by galactose 6-phosphate isomerase (Gal6PI); and converting the Gal6P to galactose catalyzed by galactose 6-phosphate phosphatase (Gal6PP).
[0067] In one embodiment, the improved process of the invention is directed to the production of fructose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); and converting the F6P to fructose catalyzed by fructose 6-phosphate phosphatase (F6PP).
[0068] In one embodiment, the improved process of the invention is directed to the production of altrose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to converting the F6P to P6P catalyzed by P6PE; converting the P6P to altrose 6-phosphate (Alt6P) catalyzed by altrose 6- phosphate isomerase (Alt6PI); and converting the Alt6P produced to altrose catalyzed by altrose 6- phosphate phosphatase (Alt6PP).
[0069] In one embodiment, the improved process of the invention is directed to the production of talose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to talose 6-phosphate (Tal6P) catalyzed by talose 6-phosphate isomerase (Tal6PI); and converting the Tal6P to talose catalyzed by talose 6-phosphate phosphatase (Tal6PP).
[0070] In one embodiment, the improved process of the invention is directed to the production of sorbose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to sorbose 6-phosphate (S6P) catalyzed by sorbose 6-phosphate epimerase (S6PE); and converting the S6P to sorbose catalyzed by sorbose 6-phosphate phosphatase (S6PP).
[0071] In one embodiment, the improved process of the invention is directed to the production of gulose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the S6P to gulose 6-phosphate (Gul6P) catalyzed by gulose 6-phosphate isomerase (Gul6PI); and converting the Gul6P to gulose catalyzed by gulose 6-phosphate phosphatase (Gul6PP).
[0072] In one embodiment, the improved process of the invention is directed to the production of idose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to T6P catalyzed by F6PE; converting the T6P to sorbose 6-phosphate (S6P) catalyzed by sorbose 6-phosphate epimerase (S6PE); converting the S6P to idose 6-phosphate (I6P) catalyzed by idose 6-phosphate isomerase (I6PI); and converting the I6P to idose catalyzed by idose 6-phosphate phosphatase (I6PP).
[0073] In one embodiment, the improved process of the invention is directed to the production of tagatose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to tagatose 6- phosphate (T6P) catalyzed by fructose 6-phosphate epimerase (F6PE); converting the T6P to tagatose catalyzed by tagatose 6-phosphate phosphatase (T6PP).
[0074] In one embodiment, the improved process of the invention is directed to the production of psicose. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to fructose 6- phosphate (F6P) catalyzed by phosphoglucoisomerase (PGI); converting the F6P to psicose 6-phosphate (P6P) catalyzed by psicose 6-phosphate epimerase (P6PE); converting the P6P to psicose catalyzed by psicose 6-phosphate phosphatase (P6PP).
[0075] In one embodiment, the improved process of the invention is directed to the production of inositol. When starch or a starch derivative is the starting material, the improvement is selected from one or more of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; b) a step of converting a starch derivative to G1P, catalyzed by an aGP having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and c) a step of transglycosylating a starch derivative, catalyzed by a 4GT having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. When cellulose or a cellulose derivative is the starting material, the improvement is a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. When sucrose is the starting material, the improvement is selected from one or both of the following steps: a) a step of converting G1P to G6P, catalyzed by a PGM having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and b) a step of converting the sucrose to G1P, catalyzed by a SP having at least 90% sequence identity with any one of SEQ ID NOs: 19-25. Said process further comprises the steps of converting G6P to inositol 3- phosphate (I3P) using inositol phosphate synthase and converting I3P to inositol using inositol monophosphatase. In another embodiment, the improved process of the invention is directed to the production of inositol from sucrose.
[0076] FIG. 1 shows an enzymatic pathway for converting sucrose to the G6P intermediate. The improved processes of the invention contain one or more of the following improvements: SP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25, and PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. In preferred processes of the invention, the SP comprises the amino acid sequence of any one of SEQ ID NOs: 19-25 and the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8. In more preferred processes, the SP comprises the amino acid sequence of SEQ ID NO: 21 and the PGM comprises the amino acid sequence of SEQ ID NO: 8.
[0077] FIG. 2 is a schematic diagram showing an enzymatic pathway converting a starch derivative, maltodextrin, to the G6P intermediate. The following abbreviations are used: IA, isoamylase; PA, pullulanase; aGP, alpha-glucan phosphorylase or starch phosphorylase; 4GT, 4-glucan transferase; and PGM, phosphoglucomutase. The improved processes of the invention contain one or more of the following improvements: PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17. In preferred processes of the invention, the PGM comprises the amino acid sequence of any one of SEQ ID NOs: 2-8, the aGP comprises an amino acid sequence of any one of SEQ ID NOs: 10-13, and the 4GT comprises an amino acid sequence of any one of SEQ ID NOs: 15-17. In more preferred processes of the invention, the PGM comprises the amino acid sequence of SEQ ID NO: 8, the aGP comprises the amino acid sequence of SEQ ID NO: 11, and the 4GT comprises the amino acid sequence of SEQ ID NO: 17.
[0078] FIG. 3 shows an enzymatic pathway for converting cellulose to the G6P intermediate. In improved processes of the invention, PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8. Preferably, the PGM comprises the amino acid sequence of any one of SEQ ID NO: 2-8. More preferably, the PGM comprises the amino acid sequence of SEQ ID NO: 8.ln some improved processes of the invention for the production of a hexose, the one or more of the process steps are conducted in a single reaction vessel. In other improved processes of the invention, the process steps are conducted in more than one reaction vessels. Phosphate ions produced by dephosphorylation of the hexose phosphate can then be recycled in the process step of converting the starch derivative to G1P, particularly when all process steps are conducted in a single bioreactor or reaction vessel. The ability to recycle phosphate allows for non-stoichiometric amounts of phosphate to be used, which keeps reaction phosphate concentrations low. This affects the overall pathway and the overall rate of the processes, but does not limit the activity of the individual enzymes and allows for overall efficiency of the hexose making processes.
[0079] In an improved process of the invention for the production of a hexose, the process steps are conducted ATP-free, NAD(P)(H)-free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction. In improved processes of the invention, the process steps are conducted under at least one of the following process conditions: at a temperature ranging from about 37"C to about 85"C, at a pH ranging from about 5.0 to about 8.0, or for about 0.5 hours to about 48 hours, or as a continuous process.
[0080] For example, reaction phosphate concentrations in each of the processes can range from about 0 mM to about 300 mM, from about 0.1 mM to about 150 mM, from about 1 mM to about 50 mM, preferably from about 5 mM to about 50 mM, or more preferably from about 10 mM to about 50 mM. For instance, the reaction phosphate concentration in each of the porcesses can be about 0.1 mM, about 0.5 mM, about 1 mM, about 1.5 mM, about 2 mM, about 2.5 mM, about 5 mM, about 6 mM, about 7 mM, about 8 mM, about 9 mM, about 10 mM, about 15 mM, about 20 mM, about 25 mM, about 30 mM, about 35 mM, about 40 mM, about 45 mM, about 50 mM, or about 55 mM. Low phosphate concentration results in decreased production costs due to low total phosphate and thus lowered cost of phosphate removal. It also prevents inhibition of phosphatases or other enzymes in the process by high concentrations of free phosphate and decreases the potential for phosphate pollution.
[0081] Furthermore, each of the processes disclosed herein can be conducted without added ATP as a source of phosphate, i.e., ATP-free. Each of the processes can also be conducted without having to add NAD(P)(H), I.e., NAD(P)(H)-free.
[0082] Any suitable biologically compatible buffering agent known in the art can be used in each of the processes of the invention, such as HEPES, PBS, BIS-TRIS, MOPS, DIPSO, Trizma, etc. The reaction buffer for the processes according to the invention can have a pH ranging from 5.0-8.0. More preferably, the reaction buffer pH can range from about 6.0 to about 7.3. For example, the reaction buffer pH can be 6.0, 6.2, 6.4, 6.6, 6.8, 7.0, 7.2, or 7.3.
[0083] The reaction buffer can also contain divalent metal cations. In some processes, the steps are conducted in the presence of a divalent metal cation selected from the group consisting of Mg2*, Zn24, Ca24, Co24, Mn24, and combinations thereof. As known in the art, suitable salts may be used to introduce the desired metal cation.
[0084] In each of the processes of the invention the reaction temperature at which the process steps are conducted can range from 37-85"C. More preferably, the steps can be conducted at a temperature ranging from about 40"C to about 80"C. The temperature can be, for example, about 40"C, about 45"C, about 50"C, about 55"C, about 60"C, about 65"C, about 70"C, about 75"C, about 80"C, or about 85"C. Preferably, the reaction temperature is about 40"C, about 45"C, about 50"C, about 55"C, about 60"C, or about 65"C. More preferably the reaction temperature ranges from about about 50"C to about 55"C.
[0085] For some of the improved processes of the invention, the reaction time can be adjusted as necessary, and can range from about 0.5 hours to about 48 hours. For example, the reaction time can be about 1 hour, about 2 hours, about 4 hours, about 8 hours, about 12 hours, about 16 hours, about 18 hours, about 20 hours, about 22 hours, about 24 hours, about 26 hours, about 28 hours, about 30 hours, about 32 hours, about 34 hours, about 36 hours, about 38 hours, about 40 hours, about 42 hours, about 44 hours, about 46 hours, or about 48 hours. More preferably, the reaction time is about 24 hours.
[0086] The process can also be conducted in one or more reactors as a continuous process, with no set time limit. In a continuous process, for example, a solution maltodextrin would be pumped through a bed of immobilized enzyme at such a rate that conversion to tagatose would be complete when the solution leaves one or more columns for downstream processing. For example, 200 g/L of maltodextrin can be pumped through a column packed with immobilized enzymes (maintained at, for example, 50"C) such that when the maltodextrin leaves the column maximum tagatose yield is achieved. This methodology offers greater volumetric productivity over batch methods. This limits the time the product is in contact with the column and reaction conditions, which decreases chances of product degradation (e.g., potential hydroxymethylfurfural formation). It also increases automation in production and therefore reduces operating expenses.
[0087] The enzymes used in the steps of invention may take the form of soluble, immobilized, assembled, entrapped, or aggregated proteins. These enzymes could be adsorbed on insoluble organic or inorganic supports commonly used to improve functionality, as known in the art. These include polymeric supports such as agarose, methacrylate, polystyrene, or dextran, as well as inorganic supports such as glass, metal, or carbon-based materials. These materials are often produced with large surface- to-volume ratios and specialized surfaces that promote attachment and activity of immobilized enzymes. The enzymes might be affixed to these solid supports through covalent, ionic, or hydrophobic interactions. The enzymes could also be affixed through genetically engineered interactions such as covalent fusion to another protein or peptide sequence with affinity to the solid support, most often a polyhistidine sequence. The enzymes might be affixed either directly to the surface or surface coating, or they might be affixed to other proteins already present on the surface or surface coating. The enzymes can be immobilized all on one carrier, on individual carriers, or a combination of the two (e.g., two enzyme per carrier then mix those carriers). These variations can be mixed evenly or in defined layers to optimize turnover in a continuous process. For example, the beginning of the reactor may have a layer of aGP to ensure a high initial G1P increase. Enzymes may be immobilized all on one carrier, on individual carriers, or in groups. These enzymes may be mixed evenly or in defined layers or zones to optimize turnover.
[0088] Each of the processes according to the invention can achieve high yields due to the very favorable equilibrium constant for the overall reaction. Theoretically, up to 99% yields can be achieved if the starting material is completely converted to a phosphorylated intermediate.
[0089] Processes of the invention use low-cost starting materials and reduce production costs by decreasing costs associated with the feedstock and product separation. Starch and its derivatives are less expensive feedstocks than, for example, lactose. When a hexose is produced from lactose, glucose and other hexose(s) are separated via chromatography, which leads to higher production costs. Also, the step of hexose dephosphorylation by a phosphatase is an irreversible phosphatase reaction, regardless of the feedstock. Therefore, hexose is produced with a very high yield while effectively minimizing the subsequent product separation costs.
[0090] Processes according to the invention allow for easy recovery of hexose, and separation costs are minimized. In some preferred processes of the invention, the recovery of the desired hexose is performed without chromatographic separation. Following production of the hexose in a continuous reaction, the product is instead passed through microfiltration, ion exchange (cation then anion, possible mixed bed for polishing), concentration, crystallization, crystal isolation, and drying. Due to high yields of the hexose, the crystallization step is all that is needed to purify the hexose. To further purify the hexose prior to crystallization, one can employ nanofiltration to eliminate the risk of enzyme being present in the crystallization process and to remove any unconverted dextrins that may co-crystallize with the hexose or limit the recyclability of the mother liquor (maltodextrin, maltotetraose, maltotriose, maltose, etc.).
[0091] Improved processes of the invention also include the production of G6P from sucrose, starch or a starch derivative, or cellulose or a cellulose derivative. G6P produced from the processes of the invention may be isolated and purified by techniques known in the art, such as for example, chromatography.
Examples
[0092] The following Examples describe the improved processes using enzymes with higher activities.
[0093] Materials and Methods
[0094] All chemicals, including glucose 1-phosphate, magnesium chloride, maltodextrin DE 4-7, sodium phosphate (mono and dibasic), are reagent grade or higher and purchased from Sigma-Aldrich (St. Louis, MO, USA) or Fisher Scientific (Pittsburgh, PA, USA), unless otherwise noted. Maltotriose was purchased from Carbosynth (Berkshire, United Kingdom). E. coli BL21 (DE3) (Sigma-Aldrich, St. Louis,
MO, USA) was used as a host cell for recombinant protein expression. ZYM-5052 media including 50 mg L-l kanamycin was used for E. coli cell growth and recombinant protein expression.
[0095] Production and purification of recombinant enzymes
[0096] The E. coli BL21 (DE3) strain harboring a protein expression plasmid (pET28a) was incubated in a 1-L Erlenmeyer flask with 100 mL of ZYM-5052 media containing 50 mg L-l kanamycin. Cells were grown at 30"C with rotary shaking at 220 rpm for 16-24 hours. The cells were harvested by
centrifugation at 12"C and washed once with either 20 mM HEPES (pH 7.5) containing 20 mM HEPES (pH 7.5) containing 300 mM NaCI and 5 mM imidazole (Ni purification). The cell pellets were re-suspended in the same buffer and lysed by sonication. After centrifugation, the target proteins in the supernatants were purified via standard methods. His-tagged proteins were purified by the Profinity IMAC Ni-Charged Resin (Bio-Rad, Hercules, CA, USA) using a gradient of increasing imadazole in the previously described buffer. The purity of the recombinant proteins was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
[0097] Example 1. Improved processes with higher activity PGMs.
[0098] Various phosphogl ucom utases (PGMs) were isolated and assayed for converting G1P to G6P in a multi-step enzymatic reaction. The relative activity of each PGM was measured in the following manner. A reaction of 50 mM HEPES pH 7.2, 5 mM MgCI2, 50 mM glucose 1-phosphate, 0.02 g/L PGM, 0.1 g/L PGI, and 0.1 g/L F6PP was prepared and incubated at 50"C for 30 minutes. The reactions were stopped via filtration of enzyme with a Vivaspin 2 concentrator (10,000 MWCO). The product, fructose, was evaluated using a Hi-Plex H+ column and refractive index detector. The sample was run in 5 mM H2SO4 at 0.6 ml/min for 15.5 min at 65"C. The amount of fructose made in 30 minutes was used to determine the relative efficiencies of each PGM in the enzymatic process. The increase in activity was determined using an average of the increase in fructose peak area and peak height.
[0099] FIG. 19 is a chromatogram showing activity of various PGMs. The chromatogram shows the level of fructose made from glucose 1-phosphate after incubation with various PGMs, excess phosphoglucoisomerase, and excess fructose 6-phosphate phosphatase. PGM is the limiting enzyme of the cascade. Table 1 shows that PGMs for use in the improved processes of the invention have improved activity relative to the previously disclosed PGM from Thermococcus kodakaraensis (Uniprot ID Q68BJ6). See International Patent Application Publication WO2017/059278.
Table 1: Relative activities of PGMs
Figure imgf000031_0001
Figure imgf000032_0001
[00100] Example 2. Improved processes with higher activity aGPs.
[00101] The relative activity of each aGP was measured in the following manner. A 200 mί reaction of 25 mM sodium phosphate pH 7.2, 5 mM MgCI2, 20 g/L maltodextrin DE 4-7, 9 pg of aGP, and 0.1 g/L PGM was prepared and incubated at 50"C. Samples were mixed with 1.5 mM NAD* and 3U/mL glucose 6-phosphate dehydrogenase at various time points. The absorbance at 340 nm at each time point was used to get the rate of reaction. This rate was used to acertain the specific activity of each aGP, which was used for relative activity comparisons.
[00102] Table 2 shows that aGPs for use in the improved processes of the invention have improved activity relative to the previously disclosed aGP from Thermotoga maritima (Uniprot ID G4FEH8). See International Patent Application Publication WQ2017/059278.
Table 2: Relative activities of aGPs
Figure imgf000032_0002
[00103] Example 3. Improved processes with higher activity 4GTs. [00104] The relative activity of each 4GT was measured in the following manner. A 200 pL reaction of 50 mM sodium phosphate pH 7.2, 5 mM MgCI2, 20 g/L maltotriose, and 9 pg of 4GT was prepared and incubated at 50"C. Samples were mixed with 1.5 mM NAD+, 1 mM ATP, 1 U/mL hexokinase, and 1 U/mL glucose 6-phosphate dehydrogenase at various time points. The absorbance at 340 nm at each time point was used to get the rate of reaction. This rate was used to a certain the specific activity of each 4GT, which was used for relative activity comparisons.
[00105] Table 3 shows that 4GTs for use in the improved processes of the invention have improved activity relative to the previously disclosed 4GT from Thermococcus litoralis (Uniprot ID 032462). See International Patent Application Publication WO2018/169957.
Table 3: Relative activities of 4GTs
Figure imgf000033_0001
[00106] Example 4. Improved processes with higher activity SPs.
[00107] Various sucrose phosphorylases, assumed by homology to Uniprot ID D9TT09 (Verhaeghe et al. The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity. Appl Microbiol Biotechnol. 2014 Aug;98(16):7027-37), were isolated and assayed for converting sucrose to glucose 6-phosphate.
[00108] The relative efficiency of each SP was measured in the following manner. A reaction of 25 mM sodium phosphate pH 7.2, 5 mM MgCI^ 200 g/L sucrose, 0.15 g/L SP, 0.1 g/L PGM, 0.1 g/L PGI, and 0.3 g/L F6PP was prepared and incubated at 50"C. Samples were taken at 0 hrs 2 hrs, 6 hrs, and 8 hrs. The reactions were stopped via filtration of enzyme with a Vivaspin 2 concentrator (10,000 MWCO). The product, fructose, was evaluated using a Supel Cogel Pb column and refractive index detector. The sample was run in ultrapure water at 0.6 mL/min for 25 min at 80"C. The amount of fructose made at 2 hours is used to determine the relative activities of each SP. The amount of fructose made at 6 hrs (completion verified at 8 hrs) shows the differences in maximum achievable yield for each SP.
[00109] The effects on full conversion of sucrose to fructose were also investigated. Of the eight enzymes tested relative to the reference (below table), seven show improved activity over Uniprot ID D9TT09 and one shows unexpected advantages when making fructose from sucrose. FIG. 18 shows chromatograms of sucrose phosphorylase activities, comparing Uniprot ID D9TT09 (reference SP) to Uniprot ID F6BJS0. At 2 hours, the higher activity SP creates approximately 150% the amount of fructose as the reference SP. The bottom chromatogram compares Uniprot ID D9TT09 (reference SP) to Uniprot ID F6BJS0 in terms of maximum yield. At 6 hours (maximum yield for both reactions), the higher activity SP creates approximately 130% the amount of fructose as the reference SP. Interestingly, the relative yields do not directly correlate with relative activities. Presumably, contributing factors include product inhibition of sucrose phosphorylase by fructose, the rate of the reverse reaction (G1P + fructose O sucrose + Pi), and more broadly the equilibrium between the formation of fructose and the degradation of fructose at late stages of the reaction. The comparative SP from Thermanaerothrix daxensis (Uniprot ID A0A0N8GPZ6), with amino acid sequence as indicated in SEQ ID NO: 26, showed lower relative activity and lower meaximal yield of fructose compared to the previously disclosed SP. bl l i i i i d h i bl i ld f
Figure imgf000034_0001
Figure imgf000035_0001
[00110] Example 5. Improved enzymatic production of G6P.
[00111] To visualize improvements in enzymatic activity, the conversion of maltodextrin to G6P was carried out using previously disclosed aGP (Uniprot ID G4FEH8) and PGM (Uniprot ID Q68BJ6), and comparing that process a process using aGP (Uniprot ID D1B926) and PGM (Uniprot ID A0A150LLZ1), which have higher activities. A 200 mί reaction mixture containing 20 g/L maltodextrin DE 5, 50 mM phosphate buffer pH 7.2, 5 mM MgCI2, 0.05 g/L aGP, and 0.005 g/L PGM was incubated at 50°C for 30 minutes. The reaction was stopped via filtration of enzyme with a Vivaspin 2 concentrator (30,000 MWCO) and analyzed via HPLC (Agilent 1100 series) using an Agilent Hi-Plex H-column and refractive index detector. The sample was run in 5 mM H2SO4 at 0.6 ml/min for 15.5 minutes at 65"C. Results were not quantified as the peaks for the void, maltodextrin, and G6P are too close to quantify any individual component reliably (FIG. 17), but clearly much more G6P is created with the enzymes aGP (Uniprot ID D1B926) and PGM (Uniprot ID A0A150LLZ1).

Claims

The claimed invention is:
1. An improved process for the enzymatic production of a hexose from starch or starch derivative, wherein the improvement comprises at least one of:
a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8;
b) a step of converting a starch derivative to G1P, catalyzed by an alpha-glucan phosphorylase (aGP), wherein the aGP comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 10-13; and
c) a step of transglycosylating a starch derivative, catalyzed by a 4-alpha-glucan transferase (4GT), wherein the 4GT comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 15-17.
2. An improved process for the enzymatic production of a hexose from cellulose or cellulose derivative, wherein the improvement comprises a step of converting G1P to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8.
3. An improved process for the enzymatic production of a hexose from sucrose, wherein the improvement comprises at least one of:
a) a step of converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM), wherein the PGM comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 2-8; and
b) a step of converting sucrose to glucose 1-phosphate (G1P) using a sucrose
phosphorylase, wherein the sucrose phosphorylase comprises an amino acid sequence having at least 90% sequence identity with any one of SEQ ID NOs: 19-25.
4. The improved process any one of claims 1-3, wherein the hexose is selected from the group consisting of allose, mannose, galactose, fructose, altrose, talose, sorbose, gulose idose, allulose, inositol, and tagatose.
5. The improved process of claim 1, wherein the starch derivative is selected from the group consisting of amylose, amylopectm, soluble starch, amylodextrin, maltotriose, maltose, and maltodextrin.
6. The improved process of any one of claims 1-4, further comprising a step of dephosphorylating a hexose-phosphate using a hexose phosphate phosphatase.
7. The improved process of claim 6, wherein the process steps are conducted in a single reaction vessel.
8. The improved process of claim 7, wherein the process steps are conducted ATP-free, NAD(P)(H)- free, at a phosphate concentration from about 0.1 mM to about 150 mM, the phosphate is recycled, and/or the step of dephosphorylation of the hexose phosphate involves an energetically favorable chemical reaction.
9. The improved process of any one of claims 1-8, wherein the process steps are conducted under at least one of the following process conditions:
at a temperature ranging from about 37*C to about 85*C,
at a pH ranging from about 5.0 to about 8.0, or
for about 0.5 hours to about 48 hours, or as a continuous reaction.
10. The improved process of claim 4, further comprising a step of reduction of the hexose to its sugar alcohol.
11, The improved process of claim 4, wherein the PGM has at least 10% higher activity compared to that of PGM from Thermococcus kodakaroensis (Uniprot ID Q68BJ6).
12. The improved process of claim 1, wherein the aGP has at least 10% higher activity compared to that of aGP from Thermotoga moritima (Uniprot ID G4FEH8).
13. The improved process of claim 1, wherein the 4GT has at least 10% higher activity compared to that of 4GT from Thermococcus iitoroiis (Uniprot ID 032462).
14. The improved process of claim 3, wherein the SP has at least 10% higher activity compared to that of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09).
15. The improved process of claim 3, wherein the SP has at least 10% higher achievable yield compared to that of SP from Thermoanaerobacterium thermosaccharolyticum (Uniprot ID D9TT09).
RECTIFIED SHEET (RULE 91) ISA/US
PCT/US2019/058483 2018-10-29 2019-10-29 Enzymatic production of hexoses WO2020092315A1 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AU2019373147A AU2019373147A1 (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses
BR112021008314-6A BR112021008314A2 (en) 2018-10-29 2019-10-29 ENZYMATIC PRODUCTION OF HEXOSES
MX2021004525A MX2021004525A (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses.
EP19878605.5A EP3874032A4 (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses
KR1020217016119A KR20210096104A (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses
CN201980086138.9A CN113383072A (en) 2018-10-29 2019-10-29 Enzyme method for producing hexose
US17/287,240 US20210381014A1 (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses
CA3118101A CA3118101A1 (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses
JP2021523430A JP2022512857A (en) 2018-10-29 2019-10-29 Enzymatic production of hexose
ZA2021/02826A ZA202102826B (en) 2018-10-29 2021-04-28 Enzymatic production of hexoses

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862752061P 2018-10-29 2018-10-29
US62/752,061 2018-10-29
US201962857543P 2019-06-05 2019-06-05
US62/857,543 2019-06-05

Publications (1)

Publication Number Publication Date
WO2020092315A1 true WO2020092315A1 (en) 2020-05-07

Family

ID=70463479

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2019/058483 WO2020092315A1 (en) 2018-10-29 2019-10-29 Enzymatic production of hexoses

Country Status (12)

Country Link
US (1) US20210381014A1 (en)
EP (1) EP3874032A4 (en)
JP (1) JP2022512857A (en)
KR (1) KR20210096104A (en)
CN (1) CN113383072A (en)
AU (1) AU2019373147A1 (en)
BR (1) BR112021008314A2 (en)
CA (1) CA3118101A1 (en)
MA (1) MA54090A (en)
MX (1) MX2021004525A (en)
WO (1) WO2020092315A1 (en)
ZA (1) ZA202102826B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022169793A1 (en) * 2021-02-02 2022-08-11 Bonumose, Inc. Enzymatic enrichment of food ingredients for sugar reduction
EP3999636A4 (en) * 2019-07-17 2023-09-20 Bonumose Inc. Immobilized enzyme compositions for the production of hexoses

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115851648A (en) * 2021-09-24 2023-03-28 中国科学院天津工业生物技术研究所 Glycerol glucoside phosphorylase mutant with improved thermal stability and catalytic activity and application thereof
CN114395542B (en) * 2021-12-30 2023-07-25 南京诺云生物科技有限公司 Sucrose phosphorylase and application thereof
CN114317476B (en) * 2021-12-30 2023-07-14 南京诺云生物科技有限公司 Biocatalysis production process of glucosyl glycerine and sucrose phosphorylase thereof
CN114317477B (en) * 2021-12-30 2023-07-14 南京诺云生物科技有限公司 Sucrose phosphorylase and glucose-1-phosphoric acid production process
CN114231509B (en) * 2021-12-31 2023-07-25 南京诺云生物科技有限公司 Sucrose phosphorylase and glucosyl glycerol production process

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050051055A (en) * 2003-11-26 2005-06-01 한국생명공학연구원 α-Glucan phospholyase originated Thermus caldophilus GK24, preparative method thereof using recombinant host, and synthetic method of α-D-glucose-1-phosphate using the same
US20180057844A1 (en) * 2015-04-17 2018-03-01 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Inositol preparation method
US20180142276A1 (en) * 2015-05-12 2018-05-24 Universiteit Gent Mutant sucrose phosphorylases with improved glycosylation activity towards polyphenols
WO2018112139A1 (en) * 2016-12-14 2018-06-21 Bonumose Llc Enzymatic production of d-allulose
US20180216146A1 (en) * 2015-10-02 2018-08-02 Bonumose Llc Enzymatic synthesis of d-tagatose
WO2018169957A1 (en) * 2017-03-13 2018-09-20 Bonumose Llc Enzymatic production of hexoses

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130029384A1 (en) * 2010-04-06 2013-01-31 Universiteit Gent Thermostable sucrose phosphorylase
BR112019013853A2 (en) * 2017-01-06 2020-01-28 Greenlight Biosciences Inc production of cell-free sugars
KR20180111667A (en) * 2017-03-31 2018-10-11 씨제이제일제당 (주) A composition for preparing tagatose and Methods for producing tagatose using The Same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050051055A (en) * 2003-11-26 2005-06-01 한국생명공학연구원 α-Glucan phospholyase originated Thermus caldophilus GK24, preparative method thereof using recombinant host, and synthetic method of α-D-glucose-1-phosphate using the same
US20180057844A1 (en) * 2015-04-17 2018-03-01 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Inositol preparation method
US20180142276A1 (en) * 2015-05-12 2018-05-24 Universiteit Gent Mutant sucrose phosphorylases with improved glycosylation activity towards polyphenols
US20180216146A1 (en) * 2015-10-02 2018-08-02 Bonumose Llc Enzymatic synthesis of d-tagatose
WO2018112139A1 (en) * 2016-12-14 2018-06-21 Bonumose Llc Enzymatic production of d-allulose
WO2018169957A1 (en) * 2017-03-13 2018-09-20 Bonumose Llc Enzymatic production of hexoses

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP3874032A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3999636A4 (en) * 2019-07-17 2023-09-20 Bonumose Inc. Immobilized enzyme compositions for the production of hexoses
WO2022169793A1 (en) * 2021-02-02 2022-08-11 Bonumose, Inc. Enzymatic enrichment of food ingredients for sugar reduction

Also Published As

Publication number Publication date
AU2019373147A1 (en) 2022-03-24
EP3874032A1 (en) 2021-09-08
KR20210096104A (en) 2021-08-04
MA54090A (en) 2022-02-09
MX2021004525A (en) 2021-09-10
EP3874032A4 (en) 2023-01-11
JP2022512857A (en) 2022-02-07
CA3118101A1 (en) 2020-05-07
ZA202102826B (en) 2022-08-31
BR112021008314A2 (en) 2021-10-13
US20210381014A1 (en) 2021-12-09
CN113383072A (en) 2021-09-10

Similar Documents

Publication Publication Date Title
US20210381014A1 (en) Enzymatic production of hexoses
CN107208084B (en) The enzyme' s catalysis of D-Tag
AU2017376628B2 (en) Enzymatic production of D-allulose
US20220127653A1 (en) Enzymatic production of mannose
JP2024075570A (en) Enzymatic production of tagatose.
US20230183768A1 (en) Enzymatic production of allulose
TW201943854A (en) Enzymatic production of D-allulose
RU2820606C2 (en) Enzymatic production of tagatose
US20220235386A1 (en) Enzymatic production of fructose

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19878605

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2021523430

Country of ref document: JP

Kind code of ref document: A

Ref document number: 3118101

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112021008314

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 2019878605

Country of ref document: EP

Effective date: 20210531

REG Reference to national code

Ref country code: BR

Ref legal event code: B01E

Ref document number: 112021008314

Country of ref document: BR

Free format text: COM BASE NA PORTARIA 405 DE 21/12/2020, SOLICITA-SE QUE SEJA APRESENTADO, EM ATE 60 (SESSENTA) DIAS, NOVO CONTEUDO DE LISTAGEM DE SEQUENCIA POIS O CONTEUDO APRESENTADO NA PETICAO NO 870210039020 DE 29/04/2021 NAO ESTA EM LINGUA VERNACULA

ENP Entry into the national phase

Ref document number: 112021008314

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20210429

ENP Entry into the national phase

Ref document number: 2019373147

Country of ref document: AU

Date of ref document: 20191029

Kind code of ref document: A