WO2019228937A1 - Moyens et méthodes pour la production de glutarate - Google Patents

Moyens et méthodes pour la production de glutarate Download PDF

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Publication number
WO2019228937A1
WO2019228937A1 PCT/EP2019/063559 EP2019063559W WO2019228937A1 WO 2019228937 A1 WO2019228937 A1 WO 2019228937A1 EP 2019063559 W EP2019063559 W EP 2019063559W WO 2019228937 A1 WO2019228937 A1 WO 2019228937A1
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WIPO (PCT)
Prior art keywords
polynucleotide
seq
host cell
nucleotide sequence
amino acid
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PCT/EP2019/063559
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English (en)
Inventor
Christoph Wittmann
Judith Becker
Lars GLAESER
Christina ROHLES
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Universitaet Des Saarlandes
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Publication of WO2019228937A1 publication Critical patent/WO2019228937A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids

Definitions

  • the promotor is selected from the group consisting of EFTU (SEQ ID NO: 25), SOD (SEQ ID NO: 26), Tac (SEQ ID NO: 27), ilvC (SEQ ID NO: 28), Trp (SEQ ID NO: 29), PL (SEQ ID NO: 30), PR (SEQ ID NO: 31 ), lacUV5 (SEQ ID NO: 32), T7lac (SEQ ID NO: 33), AraBD (SEQ ID NO: 34) H3 (SEQ ID NO: 35), H4 (SEQ ID NO: 36), H5 (SEQ ID NO: 37), H17 (SEQ ID NO: 38), H28 (SEQ ID NO: 39), H30 (SEQ ID NO: 40), H34 (SEQ ID NO: 41 ), H36 (SEQ ID NO: 42) and H72 (SEQ ID NO: 43)
  • the expression control sequence may be controllable by the addition or removal of a chemical agent, e.g. lactose, IPTG (Isopropyl)
  • Item 15 Use of the polynucleotide of any one of items 1 to 4, the vector of item 5 or the host cell of any one of items 6 to 14 for the production of glutarate.
  • Item 16 A method for producing glutarate, comprising the steps:
  • the obtained derivatives were analyzed by gas chromatography-mass spectrometry (GC/MS 7890A, 5975C quadrupole detector, Agilent Technologies, Santa Clara, CA, USA) (Lange et al. 2017). In addition, pure glutarate was analyzed as reference standard.
  • gas chromatography-mass spectrometry GC/MS 7890A, 5975C quadrupole detector, Agilent Technologies, Santa Clara, CA, USA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un polynucléotide codant pour NCgl0464, une cellule hôte comprenant ledit polynucléotide et éventuellement d'autres polynucléotides. L'invention concerne également une utilisation des polynucléotides, vecteurs ou cellules hôtes pour la production de glutarate et des méthodes de production de glutarate. NCgl0464 est une 5-aminovalérate perméase à surexprimer et les autres polynucléotides peuvent coder gabT, gabA, gabB et/ou gabT à surexprimer. En outre, un polynucléotide codant lysE peut être inactivé dans la cellule hôte.
PCT/EP2019/063559 2018-05-28 2019-05-27 Moyens et méthodes pour la production de glutarate WO2019228937A1 (fr)

Applications Claiming Priority (2)

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LULU100803 2018-05-28
LU100803 2018-05-28

Publications (1)

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WO2019228937A1 true WO2019228937A1 (fr) 2019-12-05

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Citations (8)

* Cited by examiner, † Cited by third party
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EP1108790A2 (fr) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Nouveaux polynuclétides
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CN107236793A (zh) * 2017-05-31 2017-10-10 广东省妇幼保健院 一种棒状杆菌属细菌和克氏棒状杆菌多重荧光定量pcr引物、探针、试剂盒和检测方法
KR20180043219A (ko) * 2018-04-16 2018-04-27 한국외국어대학교 연구산학협력단 Gaba 생산성이 향상된 코리네박테리움속 균주의 제조 방법 및 이를 이용한 gaba의 생산 방법

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000805A2 (fr) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Proteines codant pour les genes corynebacterium glutamicum, intervenant dans la synthese de la membrane et le transport de la membrane
EP1108790A2 (fr) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Nouveaux polynuclétides
WO2003040292A2 (fr) * 2001-11-05 2003-05-15 Basf Aktiengesellschaft Genes codant pour des proteines de synthese membranaire et de transport membranaire
CN102838664B (zh) * 2011-11-21 2014-06-04 中国科学院微生物研究所 一种γ-氨基丁酸转运蛋白及其编码基因与应用
KR20130057600A (ko) * 2011-11-24 2013-06-03 한국화학연구원 재조합 미생물을 이용한 글루타릭산의 제조 방법
WO2015132213A1 (fr) * 2014-03-03 2015-09-11 Evocatal Gmbh Procédé de préparation d'acides aminocarboxyliques et d'aminoaldéhydes terminaux au moyen d'un micro-organisme recombinant
CN107236793A (zh) * 2017-05-31 2017-10-10 广东省妇幼保健院 一种棒状杆菌属细菌和克氏棒状杆菌多重荧光定量pcr引物、探针、试剂盒和检测方法
KR20180043219A (ko) * 2018-04-16 2018-04-27 한국외국어대학교 연구산학협력단 Gaba 생산성이 향상된 코리네박테리움속 균주의 제조 방법 및 이를 이용한 gaba의 생산 방법

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