WO2019137032A1

WO2019137032A1 - Reagent chamber structure

Info

Publication number: WO2019137032A1
Application number: PCT/CN2018/104106
Authority: WO
Inventors: 刘建; 吴冬; 郭金龙; 苗新利
Original assignee: 苏州长光华医生物医学工程有限公司
Priority date: 2018-01-09
Filing date: 2018-09-05
Publication date: 2019-07-18
Also published as: CN108159973A

Abstract

A reagent chamber structure, comprising: a reagent disk body (100) and a driving device (3), the driving device (3) being used to drive a rotating shaft (8) of the reagent disk body (100) to rotate; the reagent disk body (100) comprises: a base plate (4), a disk cover (1), a turntable (2), and a shaking mechanism (6); the turntable (2) and the shaking mechanism (6) are both disposed within a cavity between the base plate (4) and the disk cover (1); the shaking mechanism (6) is disposed on the base plate (4), and the turntable (2) is disposed above the shaking mechanism (6); the driving device (3) drives the rotating shaft (8) to rotate and the turntable (2) followingly rotates; the turntable (2) is provided thereon with a loading hole (22) used for placing reagent bottles (9, 10) and a magnetic bead bottle (11), the magnetic bead bottle (11) being movably mounted within the loading hole (22); when the turntable (2) rotates, the shaking mechanism (6) synchronously drives the magnetic bead bottle (11) to rotate. The reagent chamber structure evenly shakes magnetic beads, which effectively saves the layout space of the entire machine, facilitates the miniaturization of a device, and enables the temperature within the reagent disk to be constant at 2-8℃ by means of a cooling and heat dissipation assembly, thereby improving the accuracy and stability of a detection result.

Description

Reagent chamber structure

Technical field

The present application relates to the field of medical devices, and in particular to a reagent cartridge structure capable of realizing magnetic beads shaking and having a small volume.

Background technique

Chemiluminescence immunoassay (CLIA) combines highly sensitive chemiluminescence assays with highly specific immunoassays for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and Detection and analysis techniques for drugs and the like. It is usually necessary to coat the specific biological material on the magnetic beads, and then add the luminescent liquid to collect the luminescence intensity to quantitatively analyze the detected substance.

In order to ensure the activity of the reagent and the stability of the test results, the reagent and the magnetic beads need to be stored and used under a constant temperature environment. When the magnetic beads are left standing for a long time, it is easy to form a precipitate, which affects the easy consistency of the magnetic beads, thereby affecting the accuracy and stability of the detection results. In summary, the chemiluminescent device requires a reagent chamber capable of achieving a constant temperature of cooling and a magnetic bead.

Application content

The technical solution adopted by the present application to solve the technical problem thereof is: a reagent cartridge structure, comprising: a reagent disc body and a driving device, wherein the driving device is used for driving a rotating shaft of the reagent disc body;

The reagent tray body comprises: a bottom plate, a disk cover, a turntable, and a shaking mechanism, wherein the turntable and the shaking mechanism are disposed in a cavity between the bottom plate and the disk cover, and the shaking mechanism is disposed on the bottom plate, The rotating wheel is disposed above the shaking mechanism, and the driving device drives the rotating shaft to rotate and the rotating wheel follows the rotation. The rotating plate is provided with a loading hole for placing the reagent bottle and the magnetic bead bottle, and the magnetic bead bottle is movably mounted in the loading hole. The turntable rotates, and the shaking mechanism synchronously drives the magnetic bead bottle to rotate.

Further, the shaking mechanism comprises a fixed gear, a shaking gear and a shaking shaft, the fixed gear is fixedly mounted on the bottom plate, and the magnetic bead bottle is connected to the shaking gear by shaking the rotating shaft, the shaking The uniform gear is meshed with the fixed gear.

Further improvement is that a cooling and cooling component is disposed under the bottom plate.

It is further improved that the cooling and cooling component is a semiconductor refrigeration device.

It is further improved that the semiconductor refrigeration device comprises a Peltier compact, a cooling fin and a heat sink, and the Peltier compact, the cooling fin and the heat sink are connected in sequence, and the top of the Peltier compact is connected to the bottom plate.

Further improved, the Peltier compact is externally wrapped with an insulating block, and the outer edge of the cooling plate is wrapped with thermal insulation cotton.

Further improved, the bottom of the semiconductor refrigeration device is provided with a heat dissipation fan.

Further improved, the reagent tray body is wrapped with a side insulation sponge.

It is further improved that the turntable is provided with three loading holes in order from the inside to the outside.

The beneficial effects of the application are:

The reagent cartridge structure provided by the present application integrates the reagent and the magnetic bead loading in a reagent tray, and the magnetic bead bottle also rotates (rotates) while the turntable rotates (revolves), and no additional driving equipment is needed. The magnetic beads are shaken, and at the same time, the layout space of the whole machine can be effectively saved, which is advantageous for miniaturization of the device. Moreover, by cooling the heat dissipating component, the temperature inside the reagent tray is constant at 2-8 degrees Celsius, thereby improving the accuracy and stability of the detection result.

DRAWINGS

The present application is further described below in conjunction with the accompanying drawings and embodiments.

1 is a schematic view showing the structure of a reagent cartridge of the present application;

2 is a schematic structural view of a reagent cartridge of the present application;

Figure 3 is a schematic view showing the structure of a turntable of the reagent cartridge structure of the present application;

4 is a schematic view showing the appearance of a cooling and heat dissipating component of the reagent cartridge structure of the present application;

5 is a schematic structural view of a cooling and heat dissipating assembly of a reagent cartridge structure of the present application;

Figure 6 is a schematic structural view of a shaking mechanism of the reagent cartridge structure of the present application;

The reference numerals in the figure indicate:

100-reagent disk body, 1-disk cover, 2-turntable, 3-drive device, 4-base plate, 5-side insulation cotton, 6-shake mechanism, 7-cooling heat sink, 8-turn shaft, 9-reagent bottle , 10-Reagent bottle, 11-magnetic bead M bottle, 12-Pal paste block, 13-insulation block, 14-insulation cotton, 15-heatsink, 16-cooling fan, 17-shake shaft, 18-shake Uniform gear, 19-fixed gear, 20-cooling plate, 21-mounting frame, 22-loading hole.

Detailed ways

The present application will now be described in further detail with reference to the accompanying drawings. The drawings are simplified schematic diagrams, and only the basic structure of the present application is illustrated in a schematic manner, and thus only the configurations related to the present application are shown.

In the description of the application, it is to be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "previous", "after" Orientation or positional relationship of "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. The orientation or positional relationship shown in the drawings is merely for convenience of description and simplification of description, and does not indicate or imply that the device or component referred to has a specific orientation, is constructed and operated in a specific orientation, and therefore cannot be understood. In order to limit the application.

Moreover, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include one or more of the features either explicitly or implicitly. In the description of the application, "a plurality" means two or more, unless specifically defined otherwise.

In the application, the terms "installation", "connected", "connected", "fixed" and the like shall be understood broadly, and may be either a fixed connection or a detachable connection, unless expressly stated otherwise. Or integrally connected; may be mechanical connection or electrical connection; may be directly connected, or may be indirectly connected through an intermediate medium, and may be internal communication between the two elements. For those of ordinary skill in the art, the specific meaning of the above terms in the application can be understood on a case-by-case basis.

As shown in FIG. 1-3, the present application provides a reagent cartridge structure, comprising: a reagent disc body 100 and a driving device 3, wherein an output shaft of the driving device 3 is connected to a rotating shaft 8 of the reagent disc body 100 through a timing belt. Rotating with the driver, in this embodiment, the driving device 3 is a motor;

The reagent tray body 100 includes a bottom plate 4, a disk cover 1, a turntable 2, and a shaking mechanism 6. The turntable 2 and the shaking mechanism 6 are both mounted in a cavity between the bottom plate 4 and the disk cover 1, and the disk cover 1 For the internal heat preservation of the reagent tray body, the shaking mechanism 6 is mounted on the bottom plate 4, the turntable 2 is disposed above the shaking mechanism 6, the driving device 3 drives the rotating shaft 8 to rotate and the turntable 2 follows the rotation, the turntable 2 is provided with a loading hole 22 for placing the reagent bottle 9, the reagent bottle 10 and the magnetic bead bottle 11, the magnetic bead bottle 11 is movably mounted in the loading hole 22, the turntable 2 is rotated, and the shaking mechanism 6 is synchronized. The magnetic bead bottle 11 is rotated. When the structure is working, the driving device 3 drives the rotating shaft 8 to rotate, and the turntable 2 rotates accordingly. At the same time, the shaking mechanism 6 drives the magnetic bead bottle 11 to rotate, thereby realizing the magnetic beads without separately providing a driving device for the shaking mechanism. The rotation of the bottle 11; at the same time, the reagent bottle and the magnetic bead bottle are integrated in one turntable in the structure, and the overall layout space is effectively effective for several months, so that the overall structure of the reagent cartridge is miniaturized.

As shown in FIG. 6, it is further improved that the rocking mechanism 6 comprises a fixed gear 19, a rocking gear 18 and a rocking shaft 17, and the fixed gear 19 is fixedly mounted on the bottom plate 4, and the magnetic bead bottle 11 passes The rocking shaft 17 is connected to the rocking gear 18, and the rocking shaft 17 is fixed by a fixing frame 21 at the bottom of the turntable 2, and the rocking gear 18 is meshed with the fixed gear 19. In the present structure, the fixed gear 19 is fixed on the bottom plate 4 and does not rotate. When the turntable 2 is rotated, since the shaker gear 18 is meshed with the fixed gear 19, the shaker gear 18 is rotated, and the magnetic bead bottle 11 is further rotated. Rotating, the magnetic beads in the magnetic bead bottle 11 are shaken.

As shown in FIGS. 4 and 5, it is further improved that the cooling unit 7 is disposed under the bottom plate 4. The refrigerating and heat dissipating component 7 can be mounted under the bottom plate 4 to improve the heat dissipation efficiency of the bottom plate 4, thereby lowering the temperature inside the reagent disk body.

It is further improved that the cooling and heat dissipation assembly 7 is a semiconductor refrigeration device. Since the cooling and heat dissipating component 7 adopts the equipment of the semiconductor refrigeration technology, the space occupied by the entire reagent compartment and the cooling and heat dissipating component is effectively reduced, and the occupied space of the structure is further reduced.

It is further improved that the semiconductor refrigeration device comprises a Peltier block 12, a cooling sheet 20 and a heat sink 15, and the Peltier block 12, the cooling sheet 20, and the heat sink 15 are sequentially connected, and the cold end of the cooling sheet 20 is The Peltier block 12 is connected, and the hot end of the refrigerating sheet 20 is connected to the heat sink 15, and the top of the Peltier block 12 is connected to the bottom plate 4. The heat sink 15 is a heat sink that is formed by a plurality of heat sinks. Through the heat dissipating assembly, the temperature in the reagent tray body 100 can be quickly and effectively reduced to 2-8 °C.

It is further improved that the Peltier block 12 is externally wrapped with an insulating block 13 , and the outer edge of the cooling plate 20 is wrapped with a heat insulating cotton 14 . The Peltier block 12 and the cooling sheet 20 are insulated from the outside by the heat insulating block 13 and the heat insulating cotton 12, thereby greatly improving the cooling and heat dissipation effect of the cooling and cooling unit 7.

It is further improved that the bottom of the semiconductor refrigeration device is provided with a heat dissipation fan 16, and the heat dissipation fan 16 forms a strong convection to dissipate the heat of the hot end.

It is further improved that the reagent tray body 100 is externally wrapped with a side insulation sponge 5. The side heat insulating sponge 5 prevents the reagent disk body 100 from exchanging heat with the outside air to keep the inside thereof constant.

Further improved, the bottom plate 4 is wrapped with a heat insulating sponge. In order to keep the inside of the reagent tray body 100 constant.

Further, the turntable 2 is provided with three rotation loading holes 22 from the inside to the outside for respectively placing the reagent bottle 9, the reagent bottle 10 and the magnetic bead bottle 11.

In view of the above-described embodiments of the present application, various changes and modifications may be made by those skilled in the art without departing from the scope of the present invention. The technical scope of the application is not limited to the contents of the specification, and the technical scope thereof must be determined according to the scope of the claims.

Claims

A reagent cartridge structure, comprising: a reagent disc body and a driving device, wherein the driving device is configured to drive a rotating shaft of the reagent disc body;

The reagent tray body comprises: a bottom plate, a disk cover, a turntable, and a shaking mechanism, wherein the turntable and the shaking mechanism are disposed in a cavity between the bottom plate and the disk cover, and the shaking mechanism is disposed on the bottom plate, The rotating wheel is disposed above the shaking mechanism, and the driving device drives the rotating shaft to rotate and the rotating wheel follows the rotation. The rotating plate is provided with a loading hole for placing the reagent bottle and the magnetic bead bottle, and the magnetic bead bottle is movably mounted in the loading hole. The turntable rotates, and the shaking mechanism synchronously drives the magnetic bead bottle to rotate.
The reagent cartridge structure according to claim 1, wherein the shaking mechanism comprises a fixed gear, a shaking gear and a shaking shaft, the fixed gear is fixedly mounted on the bottom plate, and the magnetic bead bottle is shaken by The rotating shaft is connected to the shaking gear, and the shaking gear is meshed with the fixed gear.
The reagent cartridge structure according to claim 1, wherein a cooling and heat dissipating component is disposed under the bottom plate.
The reagent cartridge structure according to claim 3, wherein the refrigerating heat dissipating component is a semiconductor refrigerating apparatus.
The reagent cartridge structure according to claim 4, wherein the semiconductor refrigeration device comprises a Peltier compact, a cooling fin and a heat sink, and the Peltier compact, the cooling fin, and the heat sink are sequentially connected. The top of the Peltier block is connected to the bottom plate.
The reagent cartridge structure according to claim 5, wherein the Peltier compact is externally wrapped with an insulating block, and the outer edge of the cooling fin is wrapped with heat insulating cotton.
The reagent cartridge structure according to claim 5 or 6, wherein a cooling fan is disposed at a bottom of the semiconductor refrigeration device.
The reagent cartridge structure according to claim 1, wherein the reagent tray body is externally wrapped with a side insulation sponge.
The reagent cartridge structure according to claim 1, wherein the turntable is provided with three rotation loading holes in order from the inside to the outside.