WO2019119425A1

WO2019119425A1 - Raman system

Info

Publication number: WO2019119425A1
Application number: PCT/CN2017/118030
Authority: WO
Inventors: 牟涛涛; 骆磊; 黄晓庆
Original assignee: 深圳达闼科技控股有限公司
Priority date: 2017-12-22
Filing date: 2017-12-22
Publication date: 2019-06-27
Also published as: CN108700523A

Abstract

A Raman system, relating to the technical field of spectral measurement. The Raman system comprises: a Raman test device (100) and a Raman signal enhancement device (200). The Raman signal enhancement device (200) comprises a SERS mechanism (201) and a positioning mechanism (202) connected to the Raman test device (100). The SERS mechanism (201) is provided with a nano-enhanced particle effective region used for placing a sample to be tested. The positioning mechanism (202) is provided with a positioning slot (2021). The positioning slot (2021) is used for inserting and fixing the SERS mechanism (201) and exposes the nano-enhanced particle effective region when the SERS mechanism (201) is fixed. The nano-enhanced particle effective region faces a light source access opening of the Raman test device (100), and light emitted by the Raman test device (100) via the light source access opening is focused on the exposed nano-enhanced particle effective region. The Raman system performs rapid and forced focusing during Raman spectral measurement, thereby reducing focusing time and ensuring the accuracy of a measurement result.

Description

Raman system

Technical field

The present application relates to the field of spectrometry, and in particular to a Raman system.

Background technique

The Raman system can obtain the molecular structure information of the substance by obtaining the Raman scattering spectrum of the substance composition.

The current Raman spectroscopy measurement is carried out by placing a sample to be tested on a Raman enhancement chip, such as coating a liquid to be tested, and then drying it, and then placing it on the front end of a micro Raman or ordinary Raman spectrometer for measurement. The composition and content of the substance in the sample to be tested are obtained.

However, the inventors have found that at least the following problems exist in the prior art: when Raman spectroscopy is performed using the existing Raman system, the Raman enhancement chip is not fixed at each position, and the adjustment of the focal length and the lateral position is required for each measurement. And each adjustment time must exceed the measurement time, and the adjustment process is difficult to achieve a sufficient signal to noise ratio, this measurement method will undoubtedly bring a series of problems, such as: 1, a single measurement takes a long time, can not batch work 2, the signal-to-noise ratio is difficult to achieve optimal, the noise level is high; 3, the deviation between the multiple measurements due to position error is large, the consistency is poor; 4, inconvenient to measure, only suitable for laboratory operations, not suitable for the field operation.

Summary of the invention

One technical problem to be solved by some embodiments of the present application is to provide a Raman system to solve the above technical problems.

One embodiment of the present application provides a Raman system including: a Raman detection device and a Raman signal enhancement device; the Raman signal enhancement device includes a SERS mechanism and is coupled to the Raman detection device The positioning mechanism; the SERS mechanism is provided with an effective area of nano-reinforced particles, and the effective area of the nano-reinforced particles is used for placing the sample to be tested; the positioning mechanism is provided with a positioning groove for the SERS mechanism to be inserted and fixed, and can be fixed The SERS mechanism exposes the nano-enhanced particle effective region; the nano-enhanced particle effective region faces the light source entrance and exit of the Raman detecting device, and the light emitted by the Raman detecting device through the light source entrance and exit is focused on the exposed nano-enhanced particle effective region.

Compared with the prior art, the embodiment of the present application provides a Raman system capable of realizing fast and forced focusing, and inserting a SERS mechanism provided with an effective region of nano-enhanced particles into the positioning during Raman spectroscopy measurement. The fixing groove is fixed in the mechanism, and the effective area of the nano-reinforced particles is exposed when the SERS mechanism is fixed, and the effective area of the nano-reinforced particles is directed to the light source entrance and exit of the Raman detecting device, and then connected to the Raman detecting device by using a positioning mechanism, so that The light emitted by the Raman detecting device through the light source entrance and exit can be focused on the exposed effective area of the nano-enhanced particles, thereby realizing fast and forced focusing, thereby saving the focusing time, and effectively avoiding the error of manual adjustment, thereby ensuring the error. The accuracy of the measurement results.

DRAWINGS

The one or more embodiments are exemplified by the accompanying drawings in the accompanying drawings, and FIG. The figures in the drawings do not constitute a scale limitation unless otherwise stated.

1 is a schematic structural view of a Raman system according to a first embodiment of the present application;

2 is a schematic structural view of a Raman system according to a second embodiment of the present application;

3 is a schematic structural view of a Raman system according to a third embodiment of the present application.

Detailed ways

In order to make the objects, the technical solutions and the advantages of the present application more clear, some embodiments of the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It is understood that the specific embodiments described herein are merely illustrative of the application and are not intended to be limiting.

A first embodiment of the present application relates to a Raman system that primarily includes a Raman detection device and a Raman signal enhancement device. The Raman signal enhancement device includes a surface enhanced Raman scattering (SERS) mechanism and a positioning mechanism for connection to the Raman detection device.

Wherein, the SERS mechanism is provided with an effective area of nano-reinforced particles, the effective area of the nano-reinforced particles is used for placing the sample to be tested, and the positioning mechanism is provided with a positioning groove for inserting and fixing the SERS mechanism, and can be fixed in the SERS mechanism. The active area of the nano-enhanced particles is exposed.

Specifically, when Raman spectroscopy is performed, the SERS mechanism is inserted into the positioning groove opened in the positioning mechanism to fix, and the effective area of the nano-enhanced particles on the SERS mechanism is directed toward the light source entrance and exit of the Raman detecting device. The light emitted by the Raman detecting device through the light source entrance and exit is focused on the exposed effective area of the nano-enhanced particles, thereby realizing fast and forced focusing, thereby saving the focusing time, and effectively avoiding the error of manual adjustment, thereby ensuring accurate measurement results. Sex.

It should be noted that the effective area of the nano-enhanced particles disposed on the SERS mechanism provided in this embodiment is specifically located at the geometric center position of the SERS mechanism, and the effective area of the nano-enhanced particles is at least 1 mm×1 mm, thereby reducing the SERS as much as possible. The size of the mechanism saves the cost of preparation while also ensuring the performance of the SERS mechanism.

In addition, it is worth mentioning that in practical applications, the positioning mechanism can be directly fixed to the Raman detecting device, and when the Raman system is subjected to Raman spectroscopy, the SERS mechanism can be inserted into the positioning groove.

The positioning mechanism can also be detachably fixed to the Raman detecting device, and the tester can determine whether it is necessary to install the positioning mechanism and the SERS mechanism on the Raman detecting device as needed. Moreover, the positioning mechanism is detachably fixed to the Raman detecting device, so that one positioning mechanism can be installed and used on different Raman detecting devices. The specific implementation manner can be set by a person skilled in the art as needed. There are no restrictions here.

In addition, in order to perform the Raman spectral measurement, the SERS mechanism can be ejected from the positioning mechanism for easy replacement for the next measurement. The positioning mechanism provided in this embodiment is further provided with an eject button, and the eject button can usually be It is placed around the notch of the positioning slot so that after the Raman spectroscopy is completed, the tester can eject the SERS mechanism from the positioning mechanism by pressing the eject button.

It should be noted that, in practical applications, the specific shape, structure, and setting position of the eject button for ejecting the SERS mechanism from the positioning mechanism are not limited, and those skilled in the art can appropriately set the positioning mechanism according to the needs. The size and position of the positioning groove that is opened can be determined according to the focal length to be tested and the volume of the measured object, and there is no limitation here.

For ease of understanding, the following is a Raman enhancement chip with a SERS mechanism, and a Raman detection device for a handheld Raman detection spectrometer as an example, and the specific structure of the Raman system is shown in FIG. 1 .

In Figure 1, 100 is a handheld Raman detection spectrometer and 200 is a Raman signal enhancement device.

The Raman signal enhancement device 200 includes a Raman enhancement chip 201 and a positioning mechanism 202. The positioning mechanism 202 is provided with a positioning slot 2021 and an eject button 2022.

During the Raman spectroscopy measurement, the positioning mechanism 202 is fixed to the front end of the handheld Raman detection spectrometer 100 (the end at which the light source is touched), and the sample to be tested, such as liquid or powder, is applied to the Raman enhancement chip 201. In the upper nano-reinforced particle effective region, the Raman reinforcing chip 201 is then inserted into the positioning groove 2021 on the positioning mechanism 202, thereby realizing the fixed Raman reinforcing chip 201.

Since the size of the positioning mechanism 202 and the position of the positioning groove 2021 are prepared, the focal length of the light source that can be emitted by the handheld Raman detection spectrometer 100 is used. Therefore, the Raman enhancement chip 201 is inserted into the positioning groove. 2021. After the positioning mechanism 202 is fixed to the handheld Raman detection spectrometer 100, the light emitted by the handheld Raman detection spectrometer 100 through the light source entrance and exit is focused on the exposed nano-enhanced particle effective area.

After the above work is completed, the handheld Raman detection spectrometer 100 is turned on, and the Raman enhancement chip 201 information is determined by electromagnetic (magnetic stripe) or optical (two-dimensional code) method, and it is determined that the Raman enhancement chip 201 is available. When effective, the Raman spectroscopy measurement is started to obtain a Raman enhanced spectrum, so that the composition and content of the substance in the sample to be tested can be accurately obtained.

It is not difficult to find by the above description that the Raman system provided in this embodiment realizes the Raman spectroscopy measurement by inserting the Raman enhancement chip provided with the effective region of the nano-enhanced particles into the positioning groove opened on the positioning mechanism. Fixed, and exposed the effective area of the nano-enhanced particles when the SERS mechanism is fixed, the effective area of the nano-enhanced particles is directed to the light source entrance and exit of the handheld Raman detection spectrometer, and then connected with the handheld Raman detection spectrometer by using a positioning mechanism, so that the handheld Raman The light emitted by the detection spectrometer through the light source entrance and exit can be focused on the exposed effective area of the nano-enhanced particles, which solves the need for manual adjustment of the Raman enhancement chip in the existing Raman spectroscopy measurement, so that the Raman enhancement chip realizes the spatial three-dimensional focusing pair. Quasi-problem. Through the above Raman system, fast and forced focus is achieved, thereby saving the focusing time, and effectively avoiding the manual adjustment of the error phenomenon, thereby ensuring the accuracy of the measurement result.

In addition, since the Raman enhancement chip does not shake during use, the focal length is not changed, so that the Raman system provided in this embodiment can be applied to more test occasions.

A second embodiment of the present application relates to a Raman system. In this embodiment, the SERS mechanism is a Raman enhancement chip, and the Raman detection device is a micro-Raman detection spectrometer as an example, and the specific structure of the Raman system is shown in FIG. 2 .

As shown in FIG. 2, the Raman system of the present embodiment includes components that are substantially the same as those of the Raman system shown in FIG. 1. The main difference is that, in this embodiment, the Raman detecting device is microscopically pulled. Mann detection spectrometer.

Specifically, the 200 shown in FIG. 2 is only a microscope head of a microscopic Raman detection spectrometer, specifically an objective lens for observing a sample to be tested.

The usage of the Raman system provided in this embodiment is substantially the same as that of the Raman system in the first embodiment. For details of the technical solutions that are not implemented in this embodiment, refer to the description in the first embodiment. The technical solution will not be described here.

A third embodiment of the present application relates to a Raman system. This embodiment is further improved on the basis of the first embodiment. The main improvement is that the positioning mechanism is connected to the Raman detecting device through the Raman probe. The specific structure of the Raman system is shown in FIG. Show.

In Fig. 3, 100 is a Raman detection spectrometer, 200 is a Raman signal enhancement device, 300 is a Raman probe, and 400 is a laser.

The Raman signal enhancement device 200 in this embodiment has substantially the same structure as the Raman signal enhancement device 200 in the first embodiment or the second embodiment, and details are not described herein. The Raman detection spectrometer 100 and the laser 400 are both present. Commonly used devices are not described here. The role of the Raman probe 300 in the Raman system is described primarily in conjunction with FIG.

In particular, the Raman probe 300 is used to couple the laser 400 and the external optical path portion of the Raman detection spectrometer 100. The connection of the positioning mechanism 202 to the Raman detection spectrometer 100 by the Raman probe 300 can improve the optical coupling efficiency and improve the portability of the Raman detection spectrometer. In addition, the addition of the Raman signal enhancement device 200 at the front end of the Raman probe 300 allows the conventional Raman probe 300 to function as a Raman enhancement probe.

In FIG. 3, the laser 400 may specifically be a fiber laser, and the laser signal emitted therefrom is converted into a parallel laser beam by the first collimating mirror 301.

The dichroic film 302 is obliquely disposed at an angle of 45 degrees, and the parallel laser light is irradiated onto the dichroic color plate 302, reflected to the second collimating mirror 303 at a 45-degree angle, and focused on the Raman enhancement chip 201 through the window 304. The measured target, start Raman spectroscopy.

The Raman signal generated by the sample to be tested is accompanied by the laser reflected light, passes through the second collimating mirror 303, filters out 99.9% of the interference, is reflected to the dichroic color patch 302, and passes through the dichroic color patch 302.

The Raman signal light in the optical signal passing through the dichroic film 302 passes through the first filter 3051 and the second filter 3052 in the filter group 305 in an unimpeded manner, thereby further filtering the laser signal. Drop it.

The filtered Raman signal light is focused by a focusing mirror 306 into a slit of the Raman detecting spectrometer 100 for use in the next spectroscopic measurement.

In addition, it should be noted that, in the present embodiment, the filter selected in the Raman probe 300 is specifically a high-pass cut filter prepared by magnetron sputtering or plasma sputtering coating process, and in practical applications, the field The technicians can make reasonable selections as needed, and there are no restrictions here.

Through the above description, it is not difficult to find that the Raman system provided in this embodiment can achieve the optical coupling efficiency and the portability of the Raman detection spectrometer by using the Raman probe to fix the positioning mechanism and the Raman detection spectrometer. .

A person skilled in the art can understand that the above embodiments are specific embodiments of the present application, and various changes can be made in the form and details without departing from the spirit and scope of the application. range.

Claims

A Raman system, comprising: a Raman detection device and a Raman signal enhancement device;

The Raman signal enhancement device includes a surface enhanced Raman scattering SERS mechanism and a positioning mechanism for connecting with the Raman detecting device;

The SERS mechanism is provided with a nano-enhanced particle effective region, and the nano-enhanced particle effective region is used for placing the sample to be tested;

The positioning mechanism is provided with a positioning slot for inserting and fixing the SERS mechanism, and capable of exposing the effective area of the nano-reinforced particles when the SERS mechanism is fixed;

Wherein, the nano-enhanced particle effective region faces the light source entrance and exit of the Raman detecting device, and the light emitted by the Raman detecting device through the light source inlet and outlet is focused on the exposed nano-enhanced particle effective region.
The Raman system of claim 1 wherein said positioning mechanism is detachably secured to said Raman detecting device.
The Raman system according to claim 1 or 2, wherein the positioning mechanism is coupled to the Raman detecting device by a Raman probe.
The Raman system according to any one of claims 1 to 3, wherein the positioning mechanism is further provided with an eject button;

The eject button is used to eject the SERS mechanism from the positioning mechanism.
The Raman system of any of claims 1 to 4, wherein the nano-enhanced particle effective region is located at a geometric center of the SERS mechanism.
The Raman system according to any one of claims 1 to 5, wherein the nano-enhanced particle effective area is at least 1 mm x 1 mm.
The Raman system according to any one of claims 1 to 6, wherein the SERS mechanism is a Raman enhancement chip.
The Raman system according to any one of claims 1 to 6, wherein the Raman detecting device is a Raman detecting spectrometer.
The Raman system of claim 8 wherein said Raman detection spectrometer is a handheld Raman detection spectrometer.
The Raman system according to any one of claims 6 to 6, wherein the Raman detecting device is a micro Raman detecting spectrometer.