WO2018203947A3 - Engineered biosynthetic pathways for production of tyramine by fermentation - Google Patents

Engineered biosynthetic pathways for production of tyramine by fermentation Download PDF

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Publication number
WO2018203947A3
WO2018203947A3 PCT/US2018/017127 US2018017127W WO2018203947A3 WO 2018203947 A3 WO2018203947 A3 WO 2018203947A3 US 2018017127 W US2018017127 W US 2018017127W WO 2018203947 A3 WO2018203947 A3 WO 2018203947A3
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tyramine
production
fermentation
biosynthetic pathways
engineered biosynthetic
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PCT/US2018/017127
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French (fr)
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WO2018203947A9 (en
WO2018203947A2 (en
Inventor
Michael S. SIDDIQUI
Stefan DE KOK
Alexander SHEARER
Franklin LU
Cara Tracewell
Steven EDGAR
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Zymergen Inc.
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Priority to US62/455,428 priority
Application filed by Zymergen Inc. filed Critical Zymergen Inc.
Publication of WO2018203947A2 publication Critical patent/WO2018203947A2/en
Publication of WO2018203947A3 publication Critical patent/WO2018203947A3/en
Publication of WO2018203947A9 publication Critical patent/WO2018203947A9/en

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    • CCHEMISTRY; METALLURGY
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/001Amines; Imines
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12YENZYMES
    • C12Y103/00Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01012Prephenate dehydrogenase (1.3.1.12)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01002Transaldolase (2.2.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/010543-Deoxy-7-phosphoheptulonate synthase (2.5.1.54)
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    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01071Shikimate kinase (2.7.1.71)
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    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/01Carboxy-lyases (4.1.1)
    • C12Y401/01025Tyrosine decarboxylase (4.1.1.25)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y504/00Intramolecular transferases (5.4)
    • C12Y504/99Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99005Chorismate mutase (5.4.99.5)

Abstract

The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods.
PCT/US2018/017127 2017-02-06 2018-02-06 Engineered biosynthetic pathways for production of tyramine by fermentation WO2018203947A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201762455428P true 2017-02-06 2017-02-06
US62/455,428 2017-02-06

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP18758755.5A EP3577220A2 (en) 2017-02-06 2018-02-06 Engineered biosynthetic pathways for production of tyramine by fermentation
KR1020197025667A KR20190120238A (en) 2017-02-06 2018-02-06 Engineered biosynthetic pathways for the production of tyramine by fermentation
JP2019542552A JP2020513790A (en) 2017-02-06 2018-02-06 A genetically engineered biosynthetic pathway for the production of tyramine by fermentation
CA3052635A CA3052635A1 (en) 2017-02-06 2018-02-06 Engineered biosynthetic pathways for production of tyramine by fermentation
CN201880024534.4A CN110494560A (en) 2017-02-06 2018-02-06 The modified biosynthesis pathway of tyrasamine is generated by fermentation
US16/453,648 US11028416B2 (en) 2017-02-06 2019-06-26 Engineered biosynthetic pathways for production of tyramine by fermentation

Related Child Applications (1)

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US16/453,648 Continuation US11028416B2 (en) 2017-02-06 2019-06-26 Engineered biosynthetic pathways for production of tyramine by fermentation

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WO2018203947A2 WO2018203947A2 (en) 2018-11-08
WO2018203947A3 true WO2018203947A3 (en) 2019-01-17
WO2018203947A9 WO2018203947A9 (en) 2020-05-07

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US (1) US11028416B2 (en)
EP (1) EP3577220A2 (en)
JP (1) JP2020513790A (en)
KR (1) KR20190120238A (en)
CN (1) CN110494560A (en)
CA (1) CA3052635A1 (en)
WO (1) WO2018203947A2 (en)

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WO2018203947A2 (en) 2017-02-06 2018-11-08 Zymergen Inc. Engineered biosynthetic pathways for production of tyramine by fermentation
EP3656857A1 (en) * 2018-11-21 2020-05-27 Danmarks Tekniske Universitet Utilization of phosphoketolase for production of aromatic amino acid derived products
WO2020232027A1 (en) * 2019-05-13 2020-11-19 Zymergen Inc. Stabilization of amines with sulfur compounds

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WO2018203947A2 (en) 2018-11-08
US20190390236A1 (en) 2019-12-26
CA3052635A1 (en) 2018-11-08
EP3577220A2 (en) 2019-12-11
KR20190120238A (en) 2019-10-23
US11028416B2 (en) 2021-06-08
JP2020513790A (en) 2020-05-21
CN110494560A (en) 2019-11-22
WO2018203947A9 (en) 2020-05-07

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