WO2018197727A1 - Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins - Google Patents

Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins Download PDF

Info

Publication number
WO2018197727A1
WO2018197727A1 PCT/EP2018/061050 EP2018061050W WO2018197727A1 WO 2018197727 A1 WO2018197727 A1 WO 2018197727A1 EP 2018061050 W EP2018061050 W EP 2018061050W WO 2018197727 A1 WO2018197727 A1 WO 2018197727A1
Authority
WO
WIPO (PCT)
Prior art keywords
group
pyrazin
benzyl
phenylimidazo
acetate
Prior art date
Application number
PCT/EP2018/061050
Other languages
French (fr)
Inventor
Yves-Louis JANIN
Eloi Paul COUTANT
Vincent HERVIN
Glwadys GAGNOT
Yves Jacob
Sophie GOYARD
Thierry Rose
Original Assignee
Institut Pasteur
Centre National De La Recherche Scientifique
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur, Centre National De La Recherche Scientifique filed Critical Institut Pasteur
Priority to EP18722450.6A priority Critical patent/EP3615516B1/en
Priority to ES18722450T priority patent/ES2904366T3/en
Priority to JP2019558501A priority patent/JP7366752B2/en
Priority to CA3061245A priority patent/CA3061245A1/en
Priority to US16/607,058 priority patent/US11938199B2/en
Priority to DK18722450.6T priority patent/DK3615516T3/en
Priority to EP21200585.4A priority patent/EP3964501A1/en
Publication of WO2018197727A1 publication Critical patent/WO2018197727A1/en
Priority to JP2023176076A priority patent/JP2024009933A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/16—Halogen atoms; Nitro radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/20—Nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Definitions

  • the present invention is in the field of bioluminescence in biology and/or medicine.
  • the invention provides imidazopyrazine derivatives, processes for preparation thereof, and their uses as luciferins.
  • bioluminescent reporting systems made out of an expressed luciferase/photoprQtein and a luciferin, have become important tools in many research domains such as biological and biochemical studies, whole cell or animal imaging, diagnostics as well as in many types of screenings for potential biological activities o small molecules.
  • Few types of luciferases have been used as bioluminescent reporters for such assays.
  • the relatively big firefly luciferases i.e. 65 kDa
  • luciferases which requires a luciferin, oxygen as well as ATP were initially adapted and, later on, smaller (i.e. 19-35 kDa) luciferases from marine origin were often found more appropriate since they are ATP-independent and smaller.
  • Coelenterazine (1) is the natural substrate of the Cnidaria Aequorea, Mitrocoma, Obeli , and Periphylla photoproteins, or the hydroid Clytia photoprotein.
  • it is also the natural substrate of the luciferases of the sea pansy Renilla, the shrimp Oplophoms, and the planktonic copepods Gaussia and Metridia.
  • Vargula luciferin / vargulin (2) is the substrate of the luciferases of the ostracods Conchoecia and Vargula or the fish Porichthys.
  • luciferin analogues such as compounds 4-9 in combination with various forms of Renilla luciferases led to purple-shifted signals and/or more intense one.
  • bioluminescent systems providing stronger signal (with higher signal to noise ratio) for longer time.
  • the use of such systems as reporters in high-throughput screenings requires a strong bioluminescence signal for at least 30 minutes to allow for delays inherent to robotic-based procedures.
  • the stronger the signal of a given assay the more the miniaturization of this assay is possible, which is desirable when considering the cost involved in undertaking large high-throughput screenings campaigns.
  • a high initial bioluminescent intensity as well as a high "bioluminescent signal” over "chemiluminescent noise” ratio is also desirable in many instances.
  • such signal strength can enable the design of very diverse biological tools based on the use of a small luciferase (so far 19 kDa for the smallest), a luciferin, and a common microscope instead of the many tools based on the monitoring of a fluorescence signal, which requires the use of a fluorophore, such as the larger green fluorescent protein (27 kDa), and a microscope featuring a device to illuminate this fluorophore at a given wavelength in order to detect a signal at the emitting wavelength of the fluorophore.
  • a fluorophore such as the larger green fluorescent protein (27 kDa)
  • Another aim of the present invention is to provide stable precursors of luciferins, storable in convenient conditions prior to their use.
  • Another aim of the present invention is to provide an easy, versatile and reliable method of deprotection of these stable precursors of luciferins.
  • an easy method of deprotection would not require to be conducted under inert gas.
  • Another aim of the present invention is to provide a versatile and reliable process of preparation of said luciferins and luciferin precursors, which give access to the unexplored chemical space surrounding these compounds, and/or enable kilo-scale preparation and purification.
  • compounds of formula (III) are providing markedly improved bioluminescent signals, in terms of intensity, signal-to-noise ratio, and/or duration, in comparison with previously reported substrates such as furimazine (12).
  • the present invention relates to the use of a compound of following formula (I) in presence of a solution comprising a strong acid to detect, and/or measure the enzymatic activity of, a lumino enic protein, in vitro, in cellulo or ex vivo;
  • Ri represents II or a group selected from Cj-C 6 alkyl, C3-C7 cycloalkyl, C 6 -Ci 0 aryl, aralkyl and Cs-Cio-membered heteroaryl groups, said C)-C 6 alkyl, C3-C7 cycloalkyl, C Cio aryl, aralkyl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y] group;
  • R 2 represents a group selected from C 6 -Cio aryl and C 5 -C 10 -membered heteroaryl groups, said C 6 -Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y 2 group;
  • Ri and R 2 together form with the two carbon atom to which they are respectively attached a C 5 -C 7 cycloalkene group, a C4-C7 heterocycloaikene group, or a C 6 -C 10 arene, said C5-C7 cycloalkene group and C 4 -C 7 heterocycloaikene groups being fused with a C 6 -Cio arene, said C5-C7 cycloalkene group, C4-C7 heterocycloaikene group, Cg-Cio arene, C5-C7 cycloalkene group and C4-C7 heterocycloaikene groups being optionally substituted by at least one Y 12 group;
  • R 3 represents H, a C
  • R4 represents H or a group selected from Ci-C 6 alkyl and C3-C7 cycloalkyl groups, said C]-C 6 alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y 4 group;
  • R a represents H, a Ci-C 6 alkyl group, a C3-C7 cycloalkyl group, a C 6 -Cio aryl, or an aralkyl group, said Ci-C 6 alkyl group, C3-C7 cycloalkyl group, C6-C
  • Z represents a C 6 -Cio aryl, a Cs-Cio-membered heteroaryl groups, a C C 6 alkyl, a C3-
  • C7 cycloalkyl, a C4-C7 heterocycloaikyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetrahydrofuranyl and dioxolanyl, said C 6 -C]o aryl, C 5 -Ci 0 -membered heteroaryl groups, Q- C 6 alkyl, C3-C7 cycloalkyl and C4-C7 heterocycloaikyl being optionally substituted by at least one Yz group;
  • Y], Y 2 , Yi2, Y 3 , Y 4 , Y 5 , Y a and Y z groups being each independently selected from:
  • R a being as defined above;
  • Ri and 3 ⁇ 4 each independently represent H, a Cj-Ce alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C4-C 7 heterocycloalkyl group.
  • the strong acid enables the obtaining of the corresponding deprotected compound (after removal of the R 5 moiety), which is a lucifenn, i.e. in the framework of the present invention an imidazopyrazinone capable of emitting photons under basic conditions and/or in the presence of a luminogenic protein, in particular an adequate enzyme called luciferase.
  • a lucifenn i.e. in the framework of the present invention an imidazopyrazinone capable of emitting photons under basic conditions and/or in the presence of a luminogenic protein, in particular an adequate enzyme called luciferase.
  • Said luciferin that may be in presence of the strong acid, can be used diluted in a buffered media, e.g. comprising 2-(N-morpholino)ethanesulfonic acid (MES), tris(hydroxymethyl)aminomethane (TRIS), 4-(2-hydroxyethyl)- 1 -piperazineethanesulfonic acid (HEPES), and/or phosphate-buffered saline (PBS), preferably leading to a pH between 6 and 9, more preferably leading to a pH between 7 and 8, to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo
  • MES 2-(N-morpholino)ethanesulfonic acid
  • TIS tris(hydroxymethyl)aminomethane
  • HPES 4-(2-hydroxyethyl)- 1 -piperazineethanesulfonic acid
  • PBS phosphate-buffered saline
  • the strong acid is hydrochloric acid.
  • the solution comprising a strong acid is an ethanoic hydrochloric acid solution.
  • the volume fraction of hydrochloric acid (37% in water) in said solution is 0.1 to 10%, in particular about 1%.
  • the corresponding deprotected compound in presence of the solution comprising the strong acid may be directly used in buffered media known by the skilled person in the art, with no need of a neutralization prior to said use in buffered media.
  • the compound of following formula (I) is in particular contacted with a strong acid prior to be used in a buffered media to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo.
  • the compound of following formula (I) can be used in presence of a solution comprising a strong acid (enabling deprotection of said compound of following formula (I)) to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo, in particular after dilution of the deprotected compound in a buffered media (e.g. MES, TRIS, HEPES, PBS) preferably leading to a pH between 6 and 9, more preferably leading to a pH between 7 and 8.
  • a buffered media e.g. MES, TRIS, HEPES, PBS
  • R] represents H.
  • R4 represents H.
  • R 3 represents an aralkyl group or a hetaralkyl group, optionally substituted as defined above.
  • R 3 represents a benzyl, optionally substituted as defined above, in particular by at least one halogen, more particularly F.
  • R 3 represents a benzyl, substituted by at least one halogen, more particularly F.
  • R may represent a benzyl, substituted by one halogen, more particularly F, or a benzyl, substituted by two halogen that are identical or different, more particularly F and/or CI, the substitution being for example in 2,3; 2,5; 2,6 or 3,5.
  • R 3 is selected from the group consisting of:
  • R? representing in particular a phenyl optionally substituted by at least one group Y 2 selected from:
  • a C 1 -C 6 alkyl in particular a methyl
  • 3 ⁇ 4 representing H, a Ci-Cg alkyl group, a C3-C10 cycloaikyl group, an aralkyl group or an ad hoc protective group, 3 ⁇ 4 representing in particular H, Me or Bn, R; representing more particularly H;
  • R 2 being more particularly selected from the group consisting of:
  • Z is selected from phenyl, furanyl, thiophenyl, and is optionally substituted as defined in above,
  • Z representing in particular a phenyl, furanyl, or thiophenyl, optionally substituted by at least one Yz group selected from:
  • an halogen in particular F or CI
  • a -ORi group in particular a OMe group
  • R 3 represents a benzyl, optionally substituted by at least one halogen, more particularly F.
  • Z represents a furanyl, or thiophenyl, optionally substituted by at least one Yz as defined above.
  • the present invention relates to the use as defined above, of a compound of following formula (la :
  • R 3 represents an aralkyl group, in particular a benzyl, optionally substituted as defined above, in particular by at least one halogen, more particularly F, or a hetaralkyl group;
  • Z is selected from the group consisting of:
  • the luminogenic protein is a luciferase derived from Oplophoriis luciferase, more particularly the nanoKAZ luciferase.
  • the present invention relates to the use as defined above, of a compound of following formula (I V) as defined below.
  • the present invention also relates to a compound of formula (I) as defined above for use, after contacting with a solution comprising a strong acid, in a method in vivo of detecting, and/or measuring the enzymatic activity of, a luminogenic protein.
  • the present invention also relates to the use of a compound of formula (I) as defined above for the implementation of a kit comprising said compound of formula (I) and a solution comprising a strong acid.
  • the present invention also relates to a kit comprising:
  • the compound of formula (I) and the solution comprising a strong acid are in separated containers.
  • the kit as defined above further comprises a compound selected from the group consisting of a luminogenic protein, a polynucleotide encoding the luminogenic protein, a recombinant vector comprising the polynucleotide, and a transformant comprising the polynucleotide.
  • the luminogenic protein is chosen from:
  • luciferases and photoproteins as defined above or its mutated proteins, in particular the 19 kDa protein of Oplophorus luciferase (GenBank accession BAB 13776, 196 amino acids) or its mutated proteins.
  • luminogenic proteins and their mutated proteins can be produced by the methods well known from the skilled person in the art.
  • JNC Corporation Cosmo Bio
  • Wako Pure Chemicals Industries Wako Pure Chemicals Industries
  • Promega Corporation Prolume, etc.
  • the luminogenic protein corresponds to an optimized (in terms of sequence, production, stability as well as bioluminescence properties) luciferase catalytic domain (nanoKAZ, 19kDa) from the Oplophorus-derw ' ed luciferase. This optimization can be performed by well-known techniques of the one skilled in the art.
  • the luminogenic protein is an Oplophorus luciferase, in particular the nanoKAZ luciferase (NCBI GenBank reference: AB823628.1).
  • the present invention relates to a kit comprising:
  • the present invention also relates to the kit as defined above, as an in vitro or ex vivo diagnostic tool.
  • the present invention also relates to a kit as defined above for use as an in vivo diagnostic tool.
  • the present invention also relates to a method for producing luminescence, which comprises
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • the present invention also relates to a method for producing luminescence, which comprises contacting a compound of formula (I) with a solution comprising a strong acid to obtain a concentrated solution comprising a deprotected compound;
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • Said concentrated solution comprises the deprotected compound and in particular further comprises said strong acid.
  • the present invention also relates to a method of detecting luminescence in a sample comprising
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase, if not present in the sample;
  • the present invention also relates to a method of detecting luminescence in a sample comprising
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase, if not present in the sample;
  • the sample contains live cells.
  • the sample contains a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • the present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein, comprising:
  • the present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein or a animal hosting a transgenic organism expressing a luminogenic protein, comprising:
  • the present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises
  • the present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises
  • the compound of formula (I) is in particular of formula (IV) or of formula (II) as defined below.
  • the compound of formula (I) is in particular, prior to use, within a kit as defined above.
  • the methods as defined above reciting a compound of formula (I), a strong acid and optionally a buffered media can be understood as methods wherein the different parts of the above-mentioned kits are used.
  • the present invention also relates to a compound of following formula (II):
  • Ri represents H or a group selected from Cj-C 6 alkyl, C 3 -C 7 cycloalkyl, C 6 -C] 0 aryl, aralkyl and CyCio-membered heteroaryl groups, said C]-C 6 alkyl, C 3 -C 7 cycloalkyl, C 6 -Ci 0 aryl, aralkyl and Cs-Cio-m ember ed heteroaryl groups being optionally substituted by at least one Y] group;
  • R 2 represents a group selected from C 6 -C
  • Ri and R? together form with the two carbon atom to which they are respectively attached a C5-C7 cycloalkene group, a C 4 -C 7 heterocycloalkene group, or a C -C 10 arene, said C5-C7 cycloalkene group and C4-C7 heterocycloalkene groups being fused with a Ce-Qo arene, said C5-C7 cycloalkene group, C4-C7 heterocycloalkene group, C 6 -Cio arene, C5-C7 cycloalkene group and C 4 -C 7 heterocycloalkene groups being optionally substituted by at least one Yj 2 group;
  • R 3 represents H, a Ci-C 6 alkyl, an aralkyl group, a hetaralkyl group or a heterocycloalkyl-CH2- group, said Cj-Cg alkyl, aralkyl group, hetaralkyl group and heterocycloalkyl-CH2- group being optionally substituted by at least one Y 3 group;
  • R4 represents II or a group selected from C>-C 6 alkyl and C3-C7 cycloalkyl groups, said Ci-C 6 alkyl and C 3 -C7 cycloalkyl groups being optionally substituted by at least one Y 4 group;
  • R a represents H, a Q-C 6 alkyl group, a C 3 -C7 cycloalkyl group, a C 6 -Cio aryl, or an aralkyl group, said Ci-C 6 alkyl group, C3-C 7 cycloalkyl group, C 6 -Cio aryl, and aralkyl group being optionally substituted by at least one Y a group;
  • Z represents a Q-Cjo aryl, a C 5 -Ci 0 -membered heteroaryl groups, a Ci-C 6 alkyl, a C 3 - C 7 cycloalkyl, a C4-C7 heterocycloaikyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetra
  • Y), Y 2 , Y 1 2, Y 3 , Y 4 , Y 5 , Y a and Yz groups being each independently selected from:
  • R a being as defined above;
  • Ri and 3 ⁇ 4 each independently represent H, a Ci-C 6 alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C 4 -C7 heterocycloaikyl group;
  • R 2 is different from unsubstituted phenyl, and 4-hydroxy-phenyl optionally protected by an ad hoc protective group, or
  • R 3 is different from unsubstituted benzyl, or
  • Z is different from unsubstituted phenyl, 4-hydroxy-phenyl, optionally protected by an ad hoc protective group, and from unsubstituted furan.
  • the present invention relates to compounds with the general formula II and the following names:
  • the present invention also relates to a com ound of following formula (III):
  • Ri represents H or a group selected from Ci-Ce alkyl, C3-C7 cycloalkyl, C 6 -Cio aryl, araikyl and C 5 -Cio-membered heteroaryl groups, said Ci-C 6 alkyl, C3-C7 cycloalkyl, Cg-Cio aryl, araikyl and C5-C 1 o-membered heteroaryl groups being optionally substituted by at least one Yi group;
  • R 2 represents a group selected from C 6 -Cio aryl and C5-C 1 o-membered heteroaryl groups, said C 6 -Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y 2 group;
  • R 3 represents an araikyl group, said araikyl group being optionally substituted by at least one Y 3 group;
  • 3 ⁇ 4 represents H or a group selected from Ci-C 6 alkyl and C3-C7 cycloalkyl groups, said Ci-C 6 alkyl and C3-C 7 cycloalkyl groups being optionally substituted by at least one Y 4 group;
  • Z represents a group of following formula :
  • R, R', R" and R" ' each independently represent H or a group selected from:
  • Ci-C 6 alkyl group a C3-C7 cycloalkyl group
  • X represents O or S
  • , Y 2 , Y3 and Y groups being each independently selected from:
  • 3 ⁇ 4 and 3 ⁇ 4 each independently represent H, a Cj-Cg alkyl group, a C3-C 1 0 cycloalkyl group or an aralkyl group;
  • R 2 represents a group selected from C 6 - C10 aryl and Cs-Cio-membered heteroaryl groups substituted by at least an halogen group, in particular -F, R 2 representing in particular a 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R 3 represents a group selected from Cg-Cio aralkyl groups substituted by at least an halogen group, in particular F;
  • R 2 represents a group selected from
  • R, R* and R'" represent H and R" represents a Ci-C 6 alkyl group or a ⁇ ⁇ ⁇
  • R 2 does not represent a 4-HO-Ph- group, R 2 representing in particular a phenyl group or a group selected from C 6 -Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R 2 representing more particularly a 2-
  • R 3 represents a group selected from C 6 -
  • the present invention relates to a compound of formula (III) as defined above, wherein :
  • Z represents a group Z2 and R 3 represents an aralkyl group, said aralkyl group being substituted by at least one Y 3 group, provided that:
  • R 2 represents a group selected from C 6 -Cio aryl and C 5 -Cso-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R 2 representing in particular 2-F-phenyl, a 3-F- phenyl, or a 2,6-diF-phenyl group; or R 3 represents a group selected from C 6 -Cio aralkyl groups substituted by at least a -F group; or
  • R 2 does not represent a 4-HO-Ph- group, R 2 representing in particular a phenyl group or a group selected from C 6 -Cio aryl and C5-C 10 - membered heteroaryl groups substituted by at least a halogen group, in particular - F, R 2 representing more particularly a 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF- phenyl group; or R 3 represents a group selected from C 6 -Cio aralkyl groups substituted by at least an halogen group, in particular -F;
  • Z represents a group of following formula : ,
  • R and R' being in particular different from H.
  • the present invention relates to a compound of formula (III) as defined above, wherein :
  • ⁇ Z represents a group Z2 and R 3 represents an aralkyl group, said aralkyl group being substituted by at least one -F group,
  • ⁇ Z represents a group Zl , R and R' being in particular different from H.
  • the present invention relates to a compound of formula (III) as defined above, wherein :
  • R represents a group selected from C-6-Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R 2 representing in particular 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R 3 represents a group selected from Ce-Qo aralkyl groups substituted by at least a - F group ; or
  • R 2 does not represent a 4-HO-Ph- group, R 2 representing in particular a phenyl group or a group selected from C 6 -Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R 2 representing more particularly a 2- F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R 3 represents a group selected from C 6 - Cio aralkyl groups substituted by at least an halogen group, in particular -F;
  • ⁇ R 2 represents a group selected from C 6 -Cio aryl and Cs-Cio-membered heteroaryl groups, said Ce-Cio aryl and C 5 -Cio-membered heteroaryl groups being optionally substituted by at least one Y 2 group;
  • ⁇ Z represents a group Zl , R and R' being in particular different from H.
  • Rj represents H.
  • R4 represents H.
  • R represents a benzyl group, optionally substituted as defined above, in particular by at least one halogen, more particularly F.
  • R 3 represents a benzyl, substituted by at least one halogen, more particularly F.
  • R 3 may represent a benzyl, substituted one halogen, more particularly F, or a benzyl, substituted by two halogen that are identical or different, more particularly F and/or CI, the substitution being for example in 2,3; 2,5; 2,6 or 3,5.
  • R is selected from the group consisting of:
  • R 2 represents a phenyl, optionally substituted as defined above,
  • R 2 representing in particular a phenyl optionally substituted by at least a halogen, in particular F;
  • R 2 being more particularly selected from the group consisting of: .
  • Z is selected from Zl and Z2,
  • R, R', R" and R"' each independently representing in particular H or a group selected from:
  • R 3 represents a benzyl, optionally substituted by at least one halogen, more particularly F. and Z represents a furanyl, or thiophenyi, optionally substituted by at least one Yz as defined above.
  • the compound of formula (III) is a compound of following formula (Ilia):
  • R. being in particular selected from the group consisting of:
  • R- being in particular selected from the group consisting of:
  • Z is selected from the group consisting of:
  • the present invention relates to compounds with the general formula Ilia and the following names:
  • the present invention also relates to the use of a compound of formula (III) or (Ilia) as defined above as an in vitro or ex vivo diagnostic tool.
  • the present invention also relates to a compound of formula (III) or (Ilia) as defined above for use as an in vivo diagnostic tool.
  • the present invention also relates to a method for producing luminescence, which comprises contacting a compound of formula (III) or (Ilia) as defined above with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • a luminogenic protein in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
  • the present invention also relates to a method of detecting luminescence in a sample comprising
  • a luminogenic protein in particular an
  • Oplophonis luciferase more particularly the nanoKAZ luciferase, if not present in the sample.
  • the sample contains live cells.
  • the sample contains a luminogenic protein, in particular an Oplophoras luciferase, more particularly the nanoKAZ luciferase.
  • the present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein, comprising:
  • the present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises using a polynucleotide encoding a luminogenic protein as a reporter gene and, as a luminescence substrate, a compound of formula (III) or (Ilia) as defined above.
  • the present invention relates to a compound of following formula
  • Ri, R.2, R.3, R4 and Z are as defined for formula (III) or (Ilia).
  • R5 is as defined above.
  • the present invention relates to a compound of following formula
  • R t , R 2 , R 3 , R4 and Z are as defined for formula (III) or (Ilia).
  • the present invention relates to a compound of following formula
  • R] R 2 , R 3 , R A and Z are as defined for formula (III) or (Ilia);
  • R d represents a group selected from Ci-Cg alkyl, C 3 -C cycloalkyl and aralkyl, in particular, methyl, ethyl, or benzyl.
  • the present invention relates to compounds with the general formula (IV) and the following names:
  • the present invention relates to a compound of following formula
  • Ri represents H or a group selected from Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, C 6 -Cio aryl, aralkyl and C 5 -C 1 0 -membered heteroaryl groups, said Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, C 6 -C] 0 aryl, aralkyl and C 5 -Cio-membered heteroaryl groups being optionally substituted by at least one Yi group;
  • R 2 represents a group selected from C 6 -Ci 0 aryl and Cs-Cio-membered heteroaryl groups, said C 6 -Ci 0 aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y 2 group;
  • R 3 represents an aralkyl group, said aralkyl group being optionally substituted by at least one Y 3 group;
  • Y is a leaving group, in particular an halogen, more particularly a -CI;
  • Yi, Y 2 and Y 3 groups being each independently selected from:
  • Ci-Cf alkyl group
  • C3-C7 cycloalkyl group
  • Rii represents H, a Ci-C 6 alkyl group, a C3-C 10 cycloalkyl group or an aralkyl with the proviso that said compound is not of one of the following formulae:
  • Ri represents H.
  • R3 represents a benzyl group, optionally substituted as defined above, in particular by at least one halogen, more particularly F,
  • R being more particularly selected from the group consisting of:
  • R 2 represents a phenyl, optionally substituted as defined above,
  • R 2 representing in particular a phenyl optionally substituted by at least an halogen, in particular F;
  • R being more particularly selected from the group consisting of:
  • a range of values in the form "x-y” or “x to y”, or “x through y”, include integers x, y, and the integers therebetween.
  • the phrases “1 -6", or “1 to 6” or “1 through 6” are intended to include the integers 1, 2, 3, 4, 5, and 6.
  • Preferred embodiments include each individual integer in the range, as well as any subcombination of integers.
  • preferred integers for "1-6” can include 1, 2, 3, 4, 5, 6, 1-2, 1-3, 1-4, 1- 5, 2-3, 2-4, 2-5, 2-6, etc.
  • alkyl refers to a straight-chain, or branched alkyl group having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec- butyl, tert-butyl, pentyl, isoamyl, neopentyl, 1 -ethylpropyl, 3-methylpentyl, 2,2- dimethylbutyl, 2,3-dimethylbutyl, hexyl, etc.
  • the alkyl moiety of alkyl-containing groups, such as aralkyl or O-alkyl groups has the same meaning as alkyl defined above.
  • Lower alkyl groups which are preferred, are alkyl groups as defined above which contain 1 to 4 carbons.
  • a designation such as "C1-C4 alkyl” refers to an alkyl radical containing from 1 to 4 carbon atoms.
  • cycloalkyl refers to a saturated or partially saturated mono- or bicyclic alkyl ring system containing 3 to 7 carbon atoms.
  • a designation such as “C5-C6 cycloalkyl” refers to a cycloalkyl radical containing from 5 to 6 ring carbon atoms.
  • Examples of cycloalkyl groups include such groups as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, pinenyl, and adamantanyl.
  • the cycloalkyl moiety of cycloalkyl- containing groups, such as O-cycloalkyl groups, has the same meaning as alkyl defined above.
  • aryl refers to a substituted or unsubstituted, mono- or bicyclic hydrocarbon aromatic ring system having 6 to 10 ring carbon atoms. Examples include phenyl and naphthyl. Preferred aryl groups include unsubstituted or substituted phenyl and naphthyl groups. Included within the definition of "aryl” are fused ring systems, including, for example, ring systems in which an aromatic ring is fused to a cycloalkyl ring. Examples of such fused ring systems include, for example, indane, indene, and tetrahydronaphthalene.
  • aralkyl refers to a aryl alkyl group of formula Ar-alkyl-, wherein "aryl” and “alkyl” are as defined above, Ar being in particular Ph, alkyl being in particular Me (-CH 2 -).
  • heteroaryl refers to an aromatic group containing 5 to 10 ring carbon atoms in which one or more ring carbon atoms are replaced by at least one hetero atom such as -0-, -N-, or -S-.
  • heteroaryl groups include pyrrolyl, furanyl, thienyl, pirazolyl, imidazolyl, thiazolyl, isothiazolyl, isoxazolyl, oxazolyl, oxathiolyl, oxadiazolyl, triazolyl, oxatriazolyl, furazanyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, indolyl, isoindolyl, indazolyl, benzofuranyl, isobenzofuranyl, purinyl, quinazolinyl, quinolyl, isoquinolyl, benzoimidazolyl, benzothiazolyl, benzothiophenyl, thianaphthenyl, benzoxazolyl, benzisoxazolyl, cinnolin
  • fused ring systems including, for example, ring systems in which an aromatic ring is fused to a heterocycloalkyl ring.
  • fused ring systems include, for example, phthalamide, phthaiic anhydride, indoline, isoindoline, tetrahydroisoquinoline, chroman, isochroman, chromene, and isochromene.
  • heteroarykyl refers to a heteroaryl alkyl group of formula Heteroaryl-alkyl-, wherein “heteroaryl” and “alkyl” are as defined above, alkyl being in particular Me (-CH 2 -).
  • the term "pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem complications commensurate with a reasonable benefit/risk ratio.
  • luminogenic proteins refers to luciferases and photoproteins using luciferins.
  • strong acid is meant an acid that ionizes completely in aqueous solution by losing one proton.
  • leaving group is meant a nucleofuge which retains the lone pair from its previous bond with another species, in particular in a nucleophilic substitution reaction.
  • the leaving group is for example chosen from the group consisting of halogens, in particular -I, -Br, -CI and -F, and triflate -OS(0 2 )CF 3 .
  • the present invention is directed to pharmaceutically acceptable salts of the compounds described above.
  • pharmaceutically acceptable salts includes salts of compounds of the present invention derived from the combination of such compounds with non-toxic acid.
  • Acid addition salts include inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulfuric, nitric and phosphoric acid, as well as organic acids such as acetic, citric, propionic, tartaric, glutamic, salicylic, oxalic, methanesulfonic, para-toluenesulfonic, succinic, and benzoic acid, and related inorganic and organic acids.
  • inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulfuric, nitric and phosphoric acid
  • organic acids such as acetic, citric, propionic, tartaric, glutamic, salicylic, oxalic, methanesulfonic, para-toluenesulfonic, succinic, and benzoic acid, and related inorganic and organic acids.
  • salts are included in the invention. They may serve as intermediates in the purification of the compounds, in the preparation of other salts, or in the identification and characterization of the compounds or intermediates.
  • the pharmaceutically acceptable salts of compounds of the present invention can also exist as various solvates, such as with water, methanol, ethanol, dimethyl formamide, ethyl acetate and the like. Mixtures of such solvates can also be prepared.
  • the source of such solvate can be from the solvent of crystallization, inherent in the solvent of preparation or crystallization, or adventitious to such solvent. Such solvates are within the scope of the present invention.
  • compounds of the present invention may exist in various stereoisomeric forms.
  • the compounds of the present invention include both diastereomers and enantiomers.
  • the compounds are normally prepared as racemates and can conveniently be used as such, but individual enantiomers can be isolated or synthesized by conventional techniques if so desired. Such racemates and individual enantiomers and mixtures thereof form part of the present invention.
  • Specific stereoisomers can be prepared by stereospecific synthesis using enantiomerically pure or enantiomerically enriched starting materials.
  • the specific stereoisomers of either starting materials or products can be resolved and recovered by techniques known in the art, such as resolution of racemic forms, normal, reverse-phase, and chiral chromatography, recrystallization, enzymatic resolution, or fractional recrystallization of addition salts formed by reagents used for that purpose.
  • the compounds of the present invention may be prepared in a number of methods well known to those skilled in the art, including, but not limited to those described below, or through modifications of these methods by applying standard techniques known to those skilled in the art of organic synthesis.
  • the appropriate modifications and substitutions will be readily apparent and well known or readily obtainable from the scientific literature to those skilled in the art. In particular, such methods can be found in R.C. Larock, Comprehensive Organic Transformations, Wiley-VCH Publishers, 1999. All processes disclosed in association with the present invention are contemplated to be practiced on any scale, including milligram, gram, multigram, kilogram, multikilogram or commercial industrial scale.
  • the compounds of the present invention may contain one or more asymmetrically substituted carbon atoms, and may be isolated in optically active or racemic forms.
  • optically active or racemic forms all chiral, diastereomeric, racemic forms, isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated.
  • mixtures of stereoisomers may be separated by standard techniques including, but not limited to, resolution of racemic forms, normal, reverse-phase, and chiral chromatography, preferential salt formation, recrystallization, and the like, or by chiral synthesis either from chiral starting materials or by deliberate synthesis of target chiral centers.
  • the present invention also relates to a process of preparation of a compound of formula (III) as defined above, comprising a step of conversion of a compound of formula (IV) as defined above into a compound of formula (III).
  • the process of preparation of a compound of formula (III) as defined above comprises:
  • the strong acid is hydrochloric acid.
  • the conversion of (IV) into (III) is conducted at a temperature comprised from 40°C to 90°C, for example at 50°C, in particular in a sealed tube.
  • the strong acid used for the conversion of (IV) into (III) is hydrochloric acid, in particular in solution in ethanol.
  • the conversion of (IV) into (III) is conducted at a temperature comprised from 40°C to 90°C, in particular at 50°C.
  • said compound of formula (TV) is prepared according to a process comprising:
  • said compound of formula (IV) is prepared according to a process comprising:
  • the strong base used for the conversion of (VI) into (V) is sodium hydroxide, the hydrolysis being in particular conducted at a temperature comprised from 15 to 30°C.
  • the compound selected from the group consisting of anhydrides, chlorofomates and dicarbonates is acetic or pivalic anhydride, the contacting with said compound being in particular conducted at a temperature comprised from 15 to 30°C.
  • the conversion of (VI) into (IV) is conducted at a temperature comprised from 100°C to 150°C, in particular at 140°C, in particular under microwave irradiation.
  • said compound of formula (VI) is obtained from a process comprising: i) a step of replacement of the hydroxyl group of a compound of following formula (VIII):
  • Ri , R 2 , R 3 , R4, Z and R d are as defined above,
  • a catalyst comprising copper or palladium and a ligand chosen from BINAP, Xantphos, dichloro[ l ,3-bis(2,6-di-3-pentylphenyl)imidazol-2-yIidene](3- chloropyridyl) (PEPPSI-IPentCl),
  • Y is CI
  • step vi) being a step of contacting a compound of formula (VIII) with phenylphosphonic dichloride, in particular at a temperature comprised from 60 °C and 150 °C, more particularly at 100°C.
  • Y is Br
  • step i) being a step of contacting a compound of formula (VIII) with triflic anhydride, followed by a step of contacting the product obtained in previous step with sodium bromide, in particular at a temperature comprised from 80°C and 160°C, for example at 120°C, in particular in a sealed tube.
  • step ii) is performed in presence of a base, in particular cesium carbonate.
  • the catalyst and the ligand of step ii) are palladium acetate and BINAP, respectively, said step ii) being conducted at a temperature comprised from 40°C and 80°C, more particularly at 60°C
  • said compound of formula (VIII) is obtained from a process comprising a step of dehydrogenation and aromatization of a compound of following formula (X):
  • step a is a 1 ,4-addition of aminoesters B on nitrovinyls A which is preferably achieved in the absence of solvents.
  • Step b is the reduction of the nitro group of compounds C which is preferably achieved in acidic medium using zinc.
  • Step c involves the heat-triggered cyclization of the resulting free diamine D into compound E.
  • Steps a, b and c were usually telescoped in order to minimize side reactions and potential losses in the course of purification processes.
  • Step e is a dehydrogenation/aromatization of compound E to give the hydroxypyrazine derivatives H, which is preferably achieved using sulfur in a boiling inert solvent.
  • Step j is the replacement of the hydroxyl group of compound H by a halogen to give compounds L.
  • group X being a chloride
  • the preferable reagent used to achieve this transformation is phenylphosphonic dichloride.
  • These compounds can also be prepared in two steps (i and 1) via an ester intermediate endowed with a strong leaving capacity such as the triflic esters K followed by a reaction akin to an aromatic Finkelstein reaction (Heterocycles, 1998, 1255; WO 20121 19046, 2012).
  • these 0-protected luciferins P are much more stable and can be conveniently shipped and stored for a long time at room temperature and then hydrolysed to readily generate solutions of luciferins for their immediate use. Moreover, the removal of such group is simply achieved in a vial using solutions containing a strong acid, such as hydrochloric acid, without the need of an inert atmosphere. This unprecedented use of an acid is actually instrumental in providing a degree of stability of the resulting solution as little or no product resulting from the chemoluminescence process is observed in the course of this deprotection step.
  • a strong acid such as hydrochloric acid
  • Step n is an amination reaction using a wide variety of a-amino esters B to give compounds N. This can be achieved using copper or palladium catalysts and a variety of ligands as described in the case of many halogenated aromatic or heteroaromatic derivatives (Acc. Chem. Res., 1998, 852; J. Organomet. Chem., 1999, 125; Chem. Rev., 2006, 2651 ; Angew. Chem., Int.
  • Step o is a hydrolysis of the ester function of N, preferably achieved under basic conditions to give the acids O.
  • Step p is a cyclization followed by an O-protection which is achieved by activation of the acid function of O, preferably with a variety of reagents such as anhydrides, chloroformates, or dicarbonates.
  • the step o and p can in many cases be performed in one pot (step q) using acetic acid along with acetic anhydride.
  • the compounds N or O can be transformed directly into the corresponding luciferin Q using for instance hot acetic acid and/or hydrochloric acid (step r).
  • step e the dehydrogenation aromatization of piperazin-2-ones E into 2-hydroxypyrazines H
  • the transformation of the JV-oxides J into the halogenopyrazines L via step k and the isolation of the O-protected derivatives P obtained from compounds O via step p or from compound N via step q are the only one which have no precedents.
  • the second path is endowed with a larger scope and is based on the use of a Knoevenagel reaction with diethylmalonate which, after a reduction, gives the corresponding substituted malonates. From them, as reported (Ber. Dtsch. Chem. Ges. , 1902, 3772; Org. Process. Res. Dev., 2007, 1069), a-oxime esters are readily prepared and can be reduced into the corresponding aminoesters. Again, telescoping the first three steps very often led us to properly isolate only the intermediate oximes S before their final reduction into aminoesters. As further described in detail below, less general methods, were also employed to prepare other types -oxime esters.
  • Figure 2 illustrates the bioluminescence emission over two hours of furimazine (Q-l) and hydroxyfurimazine (Q-80), as well as the imidazo[l,2- ]pyrazin-3(7H)-ones Q-84 and Q-85 of the invention.
  • Figure 3 illustrates dynamic range (relative units (RLU) versus serial dilutions) of nano AZ, which was plotted using the imidazof l ,2- ]pyrazin-3(7//)-one Q-3.
  • Figure 4 shows a comparative Lineweaver-Burk representation of the bioluminescent activity of the nanoKAZ using furimazine (Q-l) and imidazo[ 1 ,2-a]pyrazin-3(7H)-one Q-3 as substrates: the inverse of the reaction rate (relative units [RLU] / second) is plotted versus the inverse of the substrate concentration (10 5 ⁇ ⁇ '), keeping constant the concentration of the enzyme in the both cases.
  • the two dotted lines (linear regression) are converging to the same intercept with the x-axis (-1/K m ) and different intercepts with the y-axis (1/V max ).
  • the equations of the two linear regressions are indicated and the corresponding correlation factors (R 2 ) with the experimental data are reported.
  • Figure 5 compares expression and location of protein chimeras fused to the nanoKAZ in transiently transfected HEK and Jurkat cell lines, and primary human T-cells: the cytokine receptor IL7Ra:nanoKAZ at the membrane (top), the enconsin tubulin binding protein
  • EMTB:nano AZ associated with microtubules (middle), the transcription factor STAT5a:nanoKAZ dense throughout the cytoplasm and faint in the nucleus (bottom).
  • Figure 6 depicts a superposition of two ⁇ NMR spectra of the same batch of compound 8- benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate (stored at 4 °C) made on the day of its production (A) and 30 months after (B).
  • the low resolution mass spectra were obtained on an Agilent 1100 series LC/MSD system using an atmospheric electrospray ionization system and the high resolution mass spectra (HRMS) were obtained using a Waters Micromass Q-Tof with an electrospray ion source.
  • Step 1 preparation of the dimethylacetal.
  • the corresponding aldehyde (0.044 mol) and trimethylorthoformate (5.8 mL, 0.053 mol) were dissolved in methanol (7.3 mL, 0.24 mol, dried over 3A molecular sieve).
  • methanol 7.6 mL, 0.24 mol, dried over 3A molecular sieve.
  • DOWEX 50VVX8-100 ion-exchange resin 0.2 g
  • Step 2 condensation with ethyl nitroacetate, preparation of the nitroacrylates.
  • the nitroacetate (5.7 g, 0.042 mol) was stirred in acetic anhydride (5 mL, 0.053 mol) for 15 minutes.
  • Step 3 reduction with sodium borohydnde, preparation of the substituted nitroesters.
  • the crude acrylate was dispersed dissolved in isopropanol (100 mL, dried over 4A molecular sieve). To this was added portion-wise sodium borohydride (2 g, 0.053 mol) and the suspension was heated up to reflux before allowing it to cool back to room temperature.
  • Ethyl 3-(2-methoxyphenyl)-2-nitropropanoate (R-2) Obtained as an oil (6.12 g, 53 % from 2- methoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1).
  • Ethyl 3-(3-methoxyphenyl)-2-nitropropanoate (R-3) Obtained as an oil (2.34 g, 21 % from 3- mcthoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1).
  • Ethyl 3-(4-methoxyphenyl)-2-nitropropanoate (R-4) Obtained as an oil (6.95 g, 55 % from 4- methoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1), !
  • Ethyl 2-nitro-3-m-tolylpropanoate (R-6) Obtained as an oil (1.05 g, 16 % from 3- methylbenzaldehyde) after heating at 180 °C for 8h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 2/1 to 1/6) and (cyclohexane ⁇ ethyl acetate 97/3).
  • Ethyl 3-(5-ethylfuran-2-yl)-2-nitropropanoate Obtained as an oil (3.68 g, 39 % from 5-ethylfurfural) after heating at 140 °C for 2h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 2/1 to 1/6) and (cyclohexane - ethyl acetate 97/3).
  • Ethyl 3 -(furan-3 -yl)-2 -ni tropropanoate (R-l 1) Obtained as an oil (0.74 g, 34 % from furan-3 - carbaldehyde) from ethyl (E)-3-(furan-3-yl)-2-nitroacrylate prepared as described before (Org. Biomol. Chem., 2012, 524), after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1). !
  • Ethyl 2-nitro-3-(thiophen-2-yl)propanoate (R-l 2) Obtained as an oil (3.43 g, 33 % from thiophene-2-carbaldehyde) after heating at 140 °C for 4h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 3/2) and (cyclohexane - ethyl acetate 97/3 to 95/5).
  • Step 1 Knoevenagel condensation of diethyl malonate and aldehyde, preparation of substituted diethyl 2-methylenemalonate.
  • the crude substituted malonate (0.0422 mol) was dissolved in ethanol (60 mL, dried over 4 A molecular sieve) and cooled to 0°C.
  • a 21 % solution of sodium ethoxide in ethanol was added (18.4 mL, 0.0493 mol) followed by a slow addition of isoamyl nitrite (6.2 mL, 0.046 mol).
  • This solution was extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield the crude a-oxime esters further purified as described below.
  • Ethyl 3 -cyclohexyl -2-(hydroxyim ino)propanoate (S-2): Obtained as a white solid (4.34 g, 50 % from cyclohexanecarbaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1).
  • HRMS calcd for CnH 19 N0 3 Na: 236.1263; found, 236.1291.
  • Ethyl 2-(hydroxyimino)-3-(tetrahydrofuran-2-yl)propanoate (S-4) This compound was obtained when using 10 % Pd/C for the hydrogenation step and letting it run 48 hours. Out a 12.8 g batch, only a sample was purified by a chromatography (dichloromethane - ethanol 97/3) to give a white solid.
  • Ethyl 3-(2-chlorophenyl)-2-(hydroxyimino)propanoate (S-15): Out of a 13.1 g batch, using sodium boron hydride at 4°C, only a 0.5 g sample was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 4/1) and a recrystallization in cyclohexane to give a white solid (0.36 g).
  • Step 2 synthesis of diethyl 2-((5-cyclopropylfuran-2- yl)methylene)malonate: Diethyl 2-((5-bromofuran-2-yl)methylene)malonate (4.7 g, 14.8 mmol), cyclopropylboronic acid (1.66 g, 19.3 mol) and cesium carbonate (18.84 g, 57.8 mmol) were dispersed in a 95/5 mixture of toluene and water ( 120 mL).
  • Step 3 and 4 synthesis of ethyl 3-(5-cyclopropylfuran-2-yl)-2-(hydroxyimino)propanoate (S- 23): from the precedent compound, by using the general preparation of oxime esters described above, using sodium boronhydride overnight at 4°C for the reduction step, this compound was obtained as an orange solid (1.09 g, 44 % from diethyl 2-((5-cyclopropylfuran-2- yl)methylene)malonate) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1 ).
  • Step 1 preparation of diethyl 2-(4-bromobenzylidene)malonate.
  • Step 2 preparation of diethyl 2-(3-cyclopropylbenzylidene)malonate.
  • Step 4 an oximation reaction of the crude diethyl 2-(3- propylbenzyl)malonate using the general condition described above gave S-24 (0.2 g, 14 % from diethyl 2-((5-cyclopropylfuran-2-yl)methylene)malonate) as a white solid after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1).
  • Ethyl 2-(hydroxyimino)-3-phenylbutanoate (S-29): Step 1 : preparation of diethyl 2-(l- phenyIethyl)malonate: under a calcium chloride guard diethylmalonate (5.32 g, 0.033 mol) was dissolved in dry DMF (50 mL, dried over 4A molecular sieves) and 60 % sodium hydride in mineral oil (1.39 g, 0.0348 mol) was added portion-wise while maintaining the solution temperature at 20 °C with a water bath. This was stirred until the end of hydrogen evolution and (l-chloroethyl)benzene 4.8 mL, 0.036 mol) was added.
  • Step 2 reaction with isoamylnitrite. Under an inert atmosphere, 4.68 g of the resulting oil was dissolved in dry ethanol (50 mL, dried over 4 A molecular sieves) and cooled at 0 °C with ice.
  • Ethyl 2-amino-3-(4-isopropylphenyl)propanoate hydrochloride (B-33) Obtained as an oil which was immediately treated with a solution of hydrogen chloride in dioxane and concentrated to dryness to give the hydrochloride salt as a white powder (1.9 g, 91 % pure).
  • Step 1 the previously described (J. Org. Chem., 2006, 360) diethyl 2-((l,3-dioxolan-2- yl)methyl)malonate (7.8 g, 0.317 moi) was dissolved in ethanol (40 mL) and potassium hydroxide (1.78 g, 0.0317 moi) dissolved in ethanol (60 mL) was added drop wise. The solution was stirred overnight at room temperature and then briefly heated to reflux. This was concentrated, dissolved in water which was made more basic with 22 % ammonia.
  • the aqueous phase was washed with dichloromethane, made acid with 37 % hydrochloric acid, extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield pure 2-((l ,3-dioxolan-2-yl)methyl)-3- ethoxy-3-oxopropanoic acid (3.85 g, 55 %).
  • Step b preparation of ethyl (2-amino- 1 -phenyl ethyl )phenylalaninate: this oil was dispersed in a cold solution of dioxane (100 mL) and 37% hydrochloric acid (38.7 mL, 0.33 moi). Zinc dust (7.45 g, 0.11 g, ⁇ 10 ⁇ ) was added portion-wise in the course of 10 minutes. The temperature was then allowed to rise to room temperature and the suspension was stirred for 2 hours. This was diluted in water, made basic with an excess of 22 % ammonia and extracted with ethyl acetate.
  • Step c preparation of 3-benzyl-5-phenylpiperazin-2-ones E: under an argon atmosphere, this oil was heated at 140 °C for 3 hours. The ethanol was removed under vacuum and the resulting solid was subjected to purification procedures as described below. Note: whne separateted, the structure attribution of these pairs of diastereoisomers can be made by checking for the existence (or not) of a nOe effect between H-3 and H-5.
  • Cis-3-(2-methylbenzyl)-5-phenylpiperazin-2-one (E-5) This compound was obtained as a white powder (4.04 g, 26 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a dispersion in boiling cyclohexane.
  • Cis-3-(3-methylbenzyl)-5-phenylpiperazin-2-one (E-7) This compound was obtained as a white powder (3.70 g, 26 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a dispersion in boiling cyclohexane.
  • Cis-3-(2-fluorobenzyl)-5-phenylpiperazin-2-one (E-25) This racemic isomer was obtained from B-27 as a white powder (2.85 g, 31 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and a dispersion in boiling cyclohexane.
  • Cis-3-(2-methoxybenzyl)-5-phenylpiperazin-2-one (E-28) This isomer was obtained as a powder (4.03 g, still containing 5 % of the aminoester) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5).
  • Cis-3-(3-fluorobenzyl)-5-phenylpiperazin-2-one (E-30): This isomer was obtained as a powder (2.25 g, 13 %) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5) and a recrystallization in a mixture of toluene and cyclohexane.
  • ⁇ -arainoamides F from a-amino esters B.
  • the considered a-amino ester (0.065 mol, either as a free base or as a hydrochloride) was dispersed in 7 N methanol ic ammonia (50 mL). This was heated at 70 °C for 16-19 hours, concentrated to dryness and the residue was purified as further described below.
  • Step i preparation of the trifluoromethanesulfonate K.
  • the considered hydroxypyrazine (3 mmol) was dissolved in dry dichloromethane (20 mL, stabilized by amylene, dried over 4A sieve).
  • Triethylamine (0.142 mL, 3.15 mmol
  • tri flic anhydride (0.51 mL, 3.06 mmol) were then added.
  • the resulting solution was stirred for 40 minutes, diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness.
  • Step 1 Exchange reaction with sodium bromide.
  • one of these purified inflate esters (2 mmol) and dry sodium bromide (0.51 g, 5 mmol) were dispersed in dry dimethyl formamide (4 mL).
  • Triflic acid (0.16 mL, 1.8 mmol) was added, the tube was closed and heated at 120 °C overnight.
  • the resulting solution was diluted in diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness.
  • the resulting residue was then further purified as described below.
  • N-l Ethyl 2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-methylpentanoate (N-l): Obtained from L- 8 and IleOEt as an oil (0.25 g, 52 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). N.B.: A degree of racemization (about 30 %) was observed, either upon storage of IleOEt as a free base or upon the N-arylation reaction. !
  • Ethyl 2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-phenylbutanoate (N-7): Obtained from L-8 and B-41 as an oil (0.3 g, 67 %) after a a chromatography over silica gel (cyclohexane - ethyl acetate 96/4).
  • Ethyl (3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl) phenylalaninate (N-19): Obtained from L-15 and PheOEt, HC1 as an oil (0.47 g, 81 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 91/9).

Abstract

The present invention is in the field of bioluminescence in biology and/or medicine. In particular, the invention provides imidazopyrazine derivatives, processes for preparation thereof, and their uses as luciferins.

Description

IMIDAZOPYRAZINE DERIVATIVES, PROCESS FOR PREPARATION THEREOF,
AND THEIR USES AS LUCIFERINS
The present invention is in the field of bioluminescence in biology and/or medicine. In particular, the invention provides imidazopyrazine derivatives, processes for preparation thereof, and their uses as luciferins.
In the recent past, bioluminescent reporting systems, made out of an expressed luciferase/photoprQtein and a luciferin, have become important tools in many research domains such as biological and biochemical studies, whole cell or animal imaging, diagnostics as well as in many types of screenings for potential biological activities o small molecules. Few types of luciferases have been used as bioluminescent reporters for such assays. The relatively big firefly luciferases (i.e. 65 kDa) which requires a luciferin, oxygen as well as ATP were initially adapted and, later on, smaller (i.e. 19-35 kDa) luciferases from marine origin were often found more appropriate since they are ATP-independent and smaller. Many kind of calcium-binding photoproteins and luciferases found in a very diverse set of marine creatures are actually using luciferins featuring an imidazof 1 ,2-fl]pyrazin-3(7/f)- one ring system. Coelenterazine (1) is the natural substrate of the Cnidaria Aequorea, Mitrocoma, Obeli , and Periphylla photoproteins, or the hydroid Clytia photoprotein. Moreover, it is also the natural substrate of the luciferases of the sea pansy Renilla, the shrimp Oplophoms, and the planktonic copepods Gaussia and Metridia. Finally, the Vargula luciferin / vargulin (2) is the substrate of the luciferases of the ostracods Conchoecia and Vargula or the fish Porichthys.
Figure imgf000002_0001
coelenterazine fl) Vargula luciferin (2)
Extensive researches have focused on the improvement of such bioluminescent systems. The use of luciferin analogues such as compounds 4-9 in combination with various forms of Renilla luciferases led to purple-shifted signals and/or more intense one.
Figure imgf000003_0001
4: R = H, R' = H 7: R = OMe, R" = OMe
5: R = OH, R' = H 8: R = OMe, R' = F
6: R = OMe, R' = O e 9: R = OH, R' = OH
Another achievement came from the use of artificial luciferases made out of modified catalytic fragments of Oplophorus luciferase in combination with a variety of non-natural substrates. Stron ly improved signals were thus obtained with furimazine (12).
Figure imgf000003_0002
With many of these artificial luciferin/luciferase systems, improvements were seen in the intensity of the bioluminescence signal as well as in its duration in comparison with the use of the corresponding wild type luciferases and their natural substrate coelenterazine (1).
However, there is a need of bioluminescent systems providing stronger signal (with higher signal to noise ratio) for longer time. Indeed, the use of such systems as reporters in high-throughput screenings requires a strong bioluminescence signal for at least 30 minutes to allow for delays inherent to robotic-based procedures. Moreover, the stronger the signal of a given assay, the more the miniaturization of this assay is possible, which is desirable when considering the cost involved in undertaking large high-throughput screenings campaigns. In whole-cell imaging, a high initial bioluminescent intensity as well as a high "bioluminescent signal" over "chemiluminescent noise" ratio is also desirable in many instances. For instance, such signal strength can enable the design of very diverse biological tools based on the use of a small luciferase (so far 19 kDa for the smallest), a luciferin, and a common microscope instead of the many tools based on the monitoring of a fluorescence signal, which requires the use of a fluorophore, such as the larger green fluorescent protein (27 kDa), and a microscope featuring a device to illuminate this fluorophore at a given wavelength in order to detect a signal at the emitting wavelength of the fluorophore.
Furthermore, the recommended storage conditions of these luciferins (in solution or as a dry powder) require the use of very low temperature (-80°C) as solutions of such compounds have been reported to be very unstable overnight at room temperature (US 20140302539).
Accordingly, it is an object of the present invention to provide alternative to conventional luciferins, leading to substantially better bioluminescence signals in terms of intensity, signal-to-noise ratio, and/or duration.
Another aim of the present invention is to provide stable precursors of luciferins, storable in convenient conditions prior to their use.
Another aim of the present invention is to provide an easy, versatile and reliable method of deprotection of these stable precursors of luciferins. In particular, such an easy method of deprotection would not require to be conducted under inert gas.
Another aim of the present invention is to provide a versatile and reliable process of preparation of said luciferins and luciferin precursors, which give access to the unexplored chemical space surrounding these compounds, and/or enable kilo-scale preparation and purification.
Inventors have for the first time demonstrated that compounds of formula (I) can be easily deprotected by contacting them with a strong acid.
In addition, Inventors have for the first time demonstrated that a selection of imidazopyrazine derivatives yield excellent bioluminent signals.
In particular, Inventors have found that compounds of formula (III) are providing markedly improved bioluminescent signals, in terms of intensity, signal-to-noise ratio, and/or duration, in comparison with previously reported substrates such as furimazine (12).
Thus, in one aspect, the present invention relates to the use of a compound of following formula (I) in presence of a solution comprising a strong acid to detect, and/or measure the enzymatic activity of, a lumino enic protein, in vitro, in cellulo or ex vivo;
Figure imgf000004_0001
wherein : Ri represents II or a group selected from Cj-C6 alkyl, C3-C7 cycloalkyl, C6-Ci0 aryl, aralkyl and Cs-Cio-membered heteroaryl groups, said C)-C6 alkyl, C3-C7 cycloalkyl, C Cio aryl, aralkyl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y] group;
R2 represents a group selected from C6-Cio aryl and C5-C10-membered heteroaryl groups, said C6-Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
or Ri and R2 together form with the two carbon atom to which they are respectively attached a C5-C7 cycloalkene group, a C4-C7 heterocycloaikene group, or a C6-C10 arene, said C5-C7 cycloalkene group and C4-C7 heterocycloaikene groups being fused with a C6-Cio arene, said C5-C7 cycloalkene group, C4-C7 heterocycloaikene group, Cg-Cio arene, C5-C7 cycloalkene group and C4-C7 heterocycloaikene groups being optionally substituted by at least one Y12 group;
R3 represents H, a C|-C6 alkyl, an aralkyl group, a hetaralkyl group or a heterocycloalkyl-CH2- group, said C]-C6 alkyl, aralkyl group, hetaralkyl group and heterocycloalkyl-CH2- group being optionally substituted by at least one Y3 group;
R4 represents H or a group selected from Ci-C6 alkyl and C3-C7 cycloalkyl groups, said C]-C6 alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
R5 represents a -C(=0)Ra group or a C(=O)OR0 group , said C(=0)Ra group and -
C(=0)ORa group being optionally substituted by at least one Y5 group;
Ra represents H, a Ci-C6 alkyl group, a C3-C7 cycloalkyl group, a C6-Cio aryl, or an aralkyl group, said Ci-C6 alkyl group, C3-C7 cycloalkyl group, C6-C|0 aryl, and aralkyl group being optionally substituted by at least one Ya group;
Z represents a C6-Cio aryl, a Cs-Cio-membered heteroaryl groups, a C C6 alkyl, a C3-
C7 cycloalkyl, a C4-C7 heterocycloaikyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetrahydrofuranyl and dioxolanyl, said C6-C]o aryl, C5-Ci0-membered heteroaryl groups, Q- C6 alkyl, C3-C7 cycloalkyl and C4-C7 heterocycloaikyl being optionally substituted by at least one Yz group;
said Y], Y2, Yi2, Y3, Y4, Y5, Ya and Yz groups being each independently selected from:
- a Ci-C6 alkyl;
- a C3-C7 cycloalkyl;
- a C6-Cio aryl; a C5-C1 o-membered heteroaryl group;
an halogen, in particular -F;
- a -CF3 group;
- a -CN group;
- a -ORj group;
- a -OSO3H group;
- a -NRjRii group;
a guanidinyl group;
- a -C(=Q)ORa group, Ra being as defined above;
Ri and ¾ each independently represent H, a Cj-Ce alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C4-C7 heterocycloalkyl group.
The presence of the strong acid enables the obtaining of the corresponding deprotected compound (after removal of the R5 moiety), which is a lucifenn, i.e. in the framework of the present invention an imidazopyrazinone capable of emitting photons under basic conditions and/or in the presence of a luminogenic protein, in particular an adequate enzyme called luciferase.
Said luciferin, that may be in presence of the strong acid, can be used diluted in a buffered media, e.g. comprising 2-(N-morpholino)ethanesulfonic acid (MES), tris(hydroxymethyl)aminomethane (TRIS), 4-(2-hydroxyethyl)- 1 -piperazineethanesulfonic acid (HEPES), and/or phosphate-buffered saline (PBS), preferably leading to a pH between 6 and 9, more preferably leading to a pH between 7 and 8, to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo
In a particular embodiment, the strong acid is hydrochloric acid.
In a particular embodiment, the solution comprising a strong acid is an ethanoic hydrochloric acid solution.
in a particular embodiment, the volume fraction of hydrochloric acid (37% in water) in said solution is 0.1 to 10%, in particular about 1%.
Once the deprotection performed, the corresponding deprotected compound in presence of the solution comprising the strong acid may be directly used in buffered media known by the skilled person in the art, with no need of a neutralization prior to said use in buffered media. Hence, the compound of following formula (I) is in particular contacted with a strong acid prior to be used in a buffered media to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo.
In other terms, the compound of following formula (I) can be used in presence of a solution comprising a strong acid (enabling deprotection of said compound of following formula (I)) to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo, in particular after dilution of the deprotected compound in a buffered media (e.g. MES, TRIS, HEPES, PBS) preferably leading to a pH between 6 and 9, more preferably leading to a pH between 7 and 8.
In a particular embodiment, R] represents H.
In a particular embodiment, R4 represents H.
In a particular embodiment, R5 represents (C=0)Me.
In a particular embodiment, R3 represents an aralkyl group or a hetaralkyl group, optionally substituted as defined above.
In a more particular embodiment, R3 represents a benzyl, optionally substituted as defined above, in particular by at least one halogen, more particularly F.
In an even more particular embodiment, R3 represents a benzyl, substituted by at least one halogen, more particularly F. In this case, R may represent a benzyl, substituted by one halogen, more particularly F, or a benzyl, substituted by two halogen that are identical or different, more particularly F and/or CI, the substitution being for example in 2,3; 2,5; 2,6 or 3,5.
For example, R3 is selected from the group consisting of:
Figure imgf000007_0001
above,
R? representing in particular a phenyl optionally substituted by at least one group Y2 selected from:
a C 1 -C6 alkyl, in particular a methyl;
- an halogen, in particular F; a -OR; group;
¾ representing H, a Ci-Cg alkyl group, a C3-C10 cycloaikyl group, an aralkyl group or an ad hoc protective group, ¾ representing in particular H, Me or Bn, R; representing more particularly H;
R2 being more particularly selected from the group consisting of:
Figure imgf000008_0001
In a particular embodiment, Z is selected from phenyl, furanyl, thiophenyl, and is optionally substituted as defined in above,
Z representing in particular a phenyl, furanyl, or thiophenyl, optionally substituted by at least one Yz group selected from:
a C,-C6 alkyl;
a C3-C7 cycloaikyl;
an halogen, in particular F or CI;
- a -CF3 group;
a -ORi group, in particular a OMe group;
Figure imgf000008_0002
Figure imgf000009_0001
It is to be noted that the present invention includes the combination of all the embodiments listed hereabove for R|, R2, R3, R4 and/or Z.
In particular, R3 represents a benzyl, optionally substituted by at least one halogen, more particularly F. and Z represents a furanyl, or thiophenyl, optionally substituted by at least one Yz as defined above.
In a particular embodiment, the present invention relates to the use as defined above, of a compound of following formula (la :
Figure imgf000009_0002
wherein:
selected from the roup consisting of:
Figure imgf000009_0003
R3 represents an aralkyl group, in particular a benzyl, optionally substituted as defined above, in particular by at least one halogen, more particularly F, or a hetaralkyl group;
Rs is as defined above;
Z is selected from the group consisting of:
Figure imgf000009_0004
Figure imgf000010_0001
In a particular embodiment, the luminogenic protein is a luciferase derived from Oplophoriis luciferase, more particularly the nanoKAZ luciferase.
In a particular embodiment, the present invention relates to the use as defined above, of a compound of following formula (I V) as defined below.
The present invention also relates to a compound of formula (I) as defined above for use, after contacting with a solution comprising a strong acid, in a method in vivo of detecting, and/or measuring the enzymatic activity of, a luminogenic protein.
The present invention also relates to the use of a compound of formula (I) as defined above for the implementation of a kit comprising said compound of formula (I) and a solution comprising a strong acid.
The present invention also relates to a kit comprising:
i) A compound of formula (I) as defined above; and
ii) A solution comprising a strong acid.
In a particular embodiment, the compound of formula (I) and the solution comprising a strong acid are in separated containers.
In a particular embodiment, the kit as defined above further comprises a compound selected from the group consisting of a luminogenic protein, a polynucleotide encoding the luminogenic protein, a recombinant vector comprising the polynucleotide, and a transformant comprising the polynucleotide.
In a more particular embodiment of the invention, the luminogenic protein is chosen from:
- the luciferases and photoproteins found in marine organisms such as Cnidaria
Aequorea, Mitrocoma, Obelia, and Periphylla; hydroid Cfytia; sea pansy Renilla, shrimp Oplophoriis, planktonic copepods Goussia and Metridia; ostracods Conchoecio and Vargula; the fish Porichthys; these luciferases and photoproteins being in particular collected from natural sources or produced by genetic engineering;
any mutant in which amino acid sequence is mutated from the natural sequence of the luciferases and photoproteins as defined above by gene recombination technology;
a domain that catalyzes luminescence of the native luciferases and photoproteins as defined above or its mutated proteins, in particular the 19 kDa protein of Oplophorus luciferase (GenBank accession BAB 13776, 196 amino acids) or its mutated proteins.
These luminogenic proteins and their mutated proteins can be produced by the methods well known from the skilled person in the art.
Alternatively, some of them may be commercially available from JNC Corporation (Cosmo Bio), Wako Pure Chemicals Industries, Promega Corporation, Prolume, etc.
In particular, the luminogenic protein corresponds to an optimized (in terms of sequence, production, stability as well as bioluminescence properties) luciferase catalytic domain (nanoKAZ, 19kDa) from the Oplophorus-derw' ed luciferase. This optimization can be performed by well-known techniques of the one skilled in the art.
In an even more particular embodiment, the luminogenic protein is an Oplophorus luciferase, in particular the nanoKAZ luciferase (NCBI GenBank reference: AB823628.1).
In particular, the present invention relates to a kit comprising:
i) A compound of formula (I) as defined above;
ii) A solution comprising a strong acid; and
iii) A buffered media.
The present invention also relates to the kit as defined above, as an in vitro or ex vivo diagnostic tool.
The present invention also relates to a kit as defined above for use as an in vivo diagnostic tool.
The present invention also relates to a method for producing luminescence, which comprises
contacting a compound of formula (I) with a solution comprising a strong acid to obtain a deprotected compound;
contacting said deprotected compound with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
The present invention also relates to a method for producing luminescence, which comprises contacting a compound of formula (I) with a solution comprising a strong acid to obtain a concentrated solution comprising a deprotected compound;
diluting said concentrated solution comprising a deprotected compound in a buffered media to obtain a diluted solution comprising said deprotected compound;
contacting said diluted solution comprising said deprotected compound with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
Said concentrated solution comprises the deprotected compound and in particular further comprises said strong acid.
The present invention also relates to a method of detecting luminescence in a sample comprising
contacting a compound of formula (I) with a solution comprising a strong acid to obtain a deprotected compound;
contacting a sample with said deprotected compound;
optionally contacting the sample with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase, if not present in the sample; and
detecting luminescence.
The present invention also relates to a method of detecting luminescence in a sample comprising
contacting a compound of formula (1) with a solution comprising a strong acid to obtain a concentrated solution comprising a deprotected compound;
diluting said concentrated solution comprising a deprotected compound in a buffered media to obtain a diluted solution comprising said deprotected compound;
contacting a sample with said diluted solution comprising said deprotected compound;
optionally contacting the sample with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase, if not present in the sample; and
detecting luminescence.
In a particular embodiment, the sample contains live cells.
In another particular embodiment, the sample contains a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase. The present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein, comprising:
contacting a compound of formula (I) with a solution comprising a strong acid to obtain a deprotected compound;
- administrating said deprotected compound to said animal; and
detecting luminescence.
The present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein or a animal hosting a transgenic organism expressing a luminogenic protein, comprising:
- contacting a compound of formula (I) with a solution comprising a strong acid to obtain a concentrated solution comprising a deprotected compound;
diluting said concentrated solution comprising a deprotected compound in a buffered media to obtain a diluted solution comprising said deprotected compound;
administrating said diluted solution comprising said deprotected compound to said animal; and
detecting luminescence.The present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises
contacting a compound of formula (I) with a solution comprising a strong acid to obtain a deprotected compound;
- using a polynucleotide encoding a luminogenic protein as a reporter gene and, as a luminescence substrate, a deprotected compound as defined above.
The present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises
contacting a compound of formula (I) with a solution comprising a strong acid to obtain a concentrated solution comprising a deprotected compound;
diluting said concentrated solution comprising a deprotected compound in a buffered media to obtain a diluted solution comprising said deprotected compound;
using a polynucleotide encoding a luminogenic protein as a reporter gene and, as a luminescence substrate, a deprotected compound within the diluted solution as defined above.In the foregoing, the compound of formula (I) is in particular of formula (IV) or of formula (II) as defined below.
In the methods defined above, the compound of formula (I) is in particular, prior to use, within a kit as defined above. Hence, the methods as defined above reciting a compound of formula (I), a strong acid and optionally a buffered media can be understood as methods wherein the different parts of the above-mentioned kits are used.
The present invention also relates to a compound of following formula (II):
Figure imgf000014_0001
Ri represents H or a group selected from Cj-C6 alkyl, C3-C7 cycloalkyl, C6-C]0 aryl, aralkyl and CyCio-membered heteroaryl groups, said C]-C6 alkyl, C3-C7 cycloalkyl, C6-Ci0 aryl, aralkyl and Cs-Cio-m ember ed heteroaryl groups being optionally substituted by at least one Y] group;
R2 represents a group selected from C6-C |0 aryl and Cs-Cjo-membered heteroaryl groups, said Cg-Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
or Ri and R? together form with the two carbon atom to which they are respectively attached a C5-C7 cycloalkene group, a C4-C7 heterocycloalkene group, or a C -C10 arene, said C5-C7 cycloalkene group and C4-C7 heterocycloalkene groups being fused with a Ce-Qo arene, said C5-C7 cycloalkene group, C4-C7 heterocycloalkene group, C6-Cio arene, C5-C7 cycloalkene group and C4-C7 heterocycloalkene groups being optionally substituted by at least one Yj2 group;
R3 represents H, a Ci-C6 alkyl, an aralkyl group, a hetaralkyl group or a heterocycloalkyl-CH2- group, said Cj-Cg alkyl, aralkyl group, hetaralkyl group and heterocycloalkyl-CH2- group being optionally substituted by at least one Y3 group;
R4 represents II or a group selected from C>-C6 alkyl and C3-C7 cycloalkyl groups, said Ci-C6 alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
R5 represents a -C(=0)Ra group or a -C(=0)ORa group, said C(=0)Ra group and - C(~0)ORa group being optionally substituted by at least one Y5 group;
Ra represents H, a Q-C6 alkyl group, a C3-C7 cycloalkyl group, a C6-Cio aryl, or an aralkyl group, said Ci-C6 alkyl group, C3-C7 cycloalkyl group, C6-Cio aryl, and aralkyl group being optionally substituted by at least one Ya group; Z represents a Q-Cjo aryl, a C5-Ci0-membered heteroaryl groups, a Ci-C6 alkyl, a C3- C7 cycloalkyl, a C4-C7 heterocycloaikyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetrahydro furanyl and dioxolanyl, said C6-C10 aryl, C5-C)0-membered heteroaryl groups, Q- C6 alkyl, C3-C7 cycloalkyl and C4-C7 heterocycloaikyl being optionally substituted by at least one Yz group;
said Y), Y2, Y12, Y3, Y4, Y5, Ya and Yz groups being each independently selected from:
a Ci-C6 alkyl;
a C3-C7 cycloalkyl;
a C6-Cio aryl;
a C5-C1 o-membered heteroaryl group;
an halogen, in particular -F;
a -CF3 group;
a CN group;
a -ORj group;
a -OS03H group;
a -NRjRii group;
a guanidinyl group;
a C(=0)ORa group, Ra being as defined above;
Ri and ¾ each independently represent H, a Ci-C6 alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C4-C7 heterocycloaikyl group;
with the proviso that when Rj and R4 represent H, then :
R2 is different from unsubstituted phenyl, and 4-hydroxy-phenyl optionally protected by an ad hoc protective group, or
R3 is different from unsubstituted benzyl, or
Z is different from unsubstituted phenyl, 4-hydroxy-phenyl, optionally protected by an ad hoc protective group, and from unsubstituted furan.
In a particular embodiment, the present invention relates to compounds with the general formula II and the following names:
8-benzyl-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(furan-3-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-((l ,3-dioxolan-2-yl)methyl)-8-benzyl-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-(benzyloxy)benzyl)-6-(4-(benzyloxy)phenyl)imidazo[l ,2-a]pyrazin-3-yl acetate 2-benzyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-y! acetate
2-benzyl-8-methyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(fLiran-2-ylmethyl)-6-(2-methoxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(furan-3-ylmethyl)-6-(2-methoxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2-methoxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(thiophen-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(3-methylbenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-6-phenyl-2-(pyridin-2-ylmethyl)imidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(3-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-((tetrahydrofuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(2-fluorophenyl)-2-(iliran-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-((tetrahydrofuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 2-(furan-2-ylmethyl)-6-phenyl-8-((tetrahydrofuran-2-yl)methyl)imidazo[l ,2-a]p>Tazin-3-yl acetate
4-(3-acetoxy-2-(4-acetoxybenzyl)-8-benzylimidazo[ 1 ,2-a]pyrazin-6-yl)phenyl acetate
2,12-dibenzyl-5H-chromeno[4,3-e]imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(2,6-difluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-6-phenyl-2-((5-(trifluoromethyl)furan-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2,6-difluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(2-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(3-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(4-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(2-fluorophenyl)-2-((5-methylfiiran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benjzyl-6-(4-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l,2-a]pyrazin-3-yl acetate 8-benzyl-2-((5-cycIopropylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-6-(4-methoxyphenyl)-2-((5-methylfuran-2-yl)methyi)imidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-isopropylbenzyl)-6~phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(3-propylbenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(pyridin-3-ylmethyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-(4-propylbenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-methylftjran-2-yl)methyl)-6-(p-tolyl)imidazo[ l,2-a]pyrazin- 3-yl acetate 8-benzyl-2-(4-bromobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-chlorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-(m-tolyl)imidazo[ 1 ,2-a]pyrazine-3 -yl acetate 8-benzyl-2-(4-cyclopropylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzy]-2-(3-cyclopropylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(3-methoxyphenyl)-2-((5-methylfi.ran-2-yl)methyl)imidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-fluorobenzyl)-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(3-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(2-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate ,
8-benzyl-2-((5-ethylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(sec-butyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((3-methylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(2-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-benzylimidazo[ 1 ,2-a]quinoxalin- 1 -yl acetate
2,4-dibenzylimidazo[ 1 ,2-a]quinoxalin- 1 -yl acetate
8-benzyl-6-phenyl-2-( 1 -phenylethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethyloxazol-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(2,4-difiuorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(cyclohexylmethyl)-6-phenyIimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(cyclopentylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(bicyclo[2.2.1 ]heptan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 2-benzyl-8-(2-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yi acetate
2-benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
2-benzyl-8-(4-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(4-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(4-hydroxyphenyl)-2-(3-propylbenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-5-methyl-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethyli iran-2-yl)methyl)-6-(4-hydroxyphenyl)imidazo[ l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(furan-2-ylmethyl)-6-(4-hydrox>rphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(3-methylbenz.yl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-( uran-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate 8-(2-chlorobenzy])-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-8-(2-chlorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(3-fluoro-4-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-8-f2-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-phenethyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-8-(2-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-(furan-2-ylmethyl)-8-(2-methylbenzyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate 2-benzyl-8-(3-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-(furan-2-ylmethyl)-8-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 2-((5-ethylftiran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2-chlorobenzyl)-2-((5-methyli iran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-((5-ethylfiiran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(3-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,6-ditluorobenzyl)-2-(ftiran-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(3,5-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(3-chlorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(3-fluorobenzyl)-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin- 3-yl acetate
8-(3-fluorobenzyl)-2-(4-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,3-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-difluorobenzyl)-2-((5-methylfiiran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,5-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,5-difluorobenzyl)-2-((5-methylfijran-2-yl)rnethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2, 12-dibenzyl-6-fluorobenzo[f]imidazo[ 1 ,2-a]quinoxalin-3-yl acetate
12-benzyl-6-fluoro-2-(furan-2-ylmethyl)benzo[f]imidazo[l ,2-a]quinoxalin-3-yl acetate
8-(3,5-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((3-methylisoxazol-5-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((3-ethylisoxazol-5-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 2-benzyl-8-(2,3-difluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(2-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(3-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(2,3 ,5-trifluorobenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((5-methylfViran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,5-Difluorobenzyl)-2-((4,5-dimethylfliran-2-yl)methyl)-6-phenylimidazo[ l,2-a]pyrazin-3- yl acetate 8-(2,5-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,3-Diiluorobenzyl)-2-((4,5-dimethyliuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazi yl acetate
8-(3,5-Dinuorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ l )2-a]pyrazin-3- yl acetate
2-(furan-2-ylmethyl)-6-phenyl-8-(2,3,5-trifluorobenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate
8-(3,5-Difluorobenzyl)-2-((5-ethylturan-2-yl)methyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((5-ethylniran-2-yl)rnethyl)-6-phenylirnidazo[] (2-a]pyrazin-3-yl acetate
8-(2,6-Ditluorobenzyl)-2-((4,5-dimethyli iran-2-yl)methyl)-6-phenylimidazo[l ,2-a]p Tazin-3- yl acetate
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(3-iluorobenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-Benzyl-6-phenyl-2-((5-propylfuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-phenyl-2-((4,5,6,7-tetrahydrobenzofuran-2-yl)methyl)imidazo[l,2-a]pyrazin-3-yl acetate
8-(2,3-difluorobenzyl)-2-((4-ethyl-5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2- a]pyTazin-3-yl acetate
8-benzyl-2-((4-ethyl-5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-difluorobenzyl)-6-phenyl-2-((4,5,6,7-tetrahydrobenzofuran-2-yl)methyl)imidazo[l ,2- a]pyrazin-3-yl acetate
8-berizyl-2-((5-isopropyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-diiluorobenzyl)-2-((5-ethyl-4-methylfuran-2-yl)methyr)-6-phenylimidazo[ l,2- a]pyrazin-3-yl acetate
8-benzyl-2-( 1 -(i iran-2-yl)ethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[ l ,2-a]pyrazin-3-yl acetate
8-(2,3-difluorobenzyl)-2-((5-isopropyl-4-methylftiran-2-yl)methyl)-6-phenylimidazo[ 1 ,2- a]pyrazin-3-yl acetate
8-benzyl-2-(furan-2-ylmethyl)-6-(3-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(4-hydroxybenzyl)-6-(3-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate.
The present invention also relates to a com ound of following formula (III):
Figure imgf000021_0001
wherein :
Ri represents H or a group selected from Ci-Ce alkyl, C3-C7 cycloalkyl, C6-Cio aryl, araikyl and C5-Cio-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, Cg-Cio aryl, araikyl and C5-C 1 o-membered heteroaryl groups being optionally substituted by at least one Yi group;
R2 represents a group selected from C6-Cio aryl and C5-C1 o-membered heteroaryl groups, said C6-Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
R3 represents an araikyl group, said araikyl group being optionally substituted by at least one Y3 group;
¾ represents H or a group selected from Ci-C6 alkyl and C3-C7 cycloalkyl groups, said Ci-C6 alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
Z represents a group of following formula :
Figure imgf000021_0002
; or an oxazolyl, or an oxadiazolyl;
R, R', R" and R" ' each independently represent H or a group selected from:
a Ci-C6 alkyl group; a C3-C7 cycloalkyl group;
an halogen;
a -ORj group;
a - CF\ group;
X represents O or S;
said Y|, Y2, Y3 and Y , groups being each independently selected from:
a Ci-Ce alkyl group;
a C3-C7 cycloalkyl group;
an halogen;
a OR.: group;
¾ and ¾ each independently represent H, a Cj-Cg alkyl group, a C3-C10 cycloalkyl group or an aralkyl group;
provided that:
when Z=Z1 :
o at least one of R, R' and R" does not represent H; or
o when R, R' and R" represent H, then R2 represents a group selected from C6- C10 aryl and Cs-Cio-membered heteroaryl groups substituted by at least an halogen group, in particular -F, R2 representing in particular a 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from Cg-Cio aralkyl groups substituted by at least an halogen group, in particular F;
when Z=Z2,:
o at least one of R, R', R" and R' " does not represent H; or
o when R, R', R" and R'" represent H; then R2 represents a group selected from
Ce-Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing in particular 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from Cg-Cio aralkyl groups substituted by at least an halogen group, in particular - F; or
o when R, R* and R'" represent H and R" represents a Ci-C6 alkyl group or a ··■··
ORj group; then R2 does not represent a 4-HO-Ph- group, R2 representing in particular a phenyl group or a group selected from C6-Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing more particularly a 2-
F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from C6-
C10 aralkyl groups substituted by at least an halogen group, in particular -F. la a particular embodiment, the present invention relates to a compound of formula (III) as defined above, wherein :
Z represents a group Z2 and R3 represents an aralkyl group, said aralkyl group being substituted by at least one Y3 group, provided that:
o at least one of R, R', R" and R' ' ' does not represent H; or
o when R, R', R" and R'" represent H; then R2 represents a group selected from C6-Cio aryl and C5-Cso-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing in particular 2-F-phenyl, a 3-F- phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from C6-Cio aralkyl groups substituted by at least a -F group; or
o when R, R' and R'" represent H and R" represents a Ci-C6 alkyl group or a - OR, group; then R2 does not represent a 4-HO-Ph- group, R2 representing in particular a phenyl group or a group selected from C6-Cio aryl and C5-C10- membered heteroaryl groups substituted by at least a halogen group, in particular - F, R2 representing more particularly a 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF- phenyl group; or R3 represents a group selected from C6-Cio aralkyl groups substituted by at least an halogen group, in particular -F;
or
Z represents a group of following formula :
Figure imgf000023_0001
,
R and R' being in particular different from H.
In a more particular embodiment, the present invention relates to a compound of formula (III) as defined above, wherein :
❖ Z represents a group Z2 and R3 represents an aralkyl group, said aralkyl group being substituted by at least one -F group,
or
❖ Z represents a group Zl , R and R' being in particular different from H.
In a particular embodiment, the present invention relates to a compound of formula (III) as defined above, wherein :
❖ R3 represents an aralkyl group, said aralkyl group being substituted by at least one Y3 group, provided that when Z=Z2:
o at least one of R, R\ R" and R'" does not represent H; or o when R, R', R" and R'" represent H; then R? represents a group selected from C-6-Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing in particular 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from Ce-Qo aralkyl groups substituted by at least a - F group ; or
o when R, R' and R"* represent H and R" represents a Ci-C6 alkyl group or a - ORj group; then R2 does not represent a 4-HO-Ph- group, R2 representing in particular a phenyl group or a group selected from C6-Cio aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing more particularly a 2- F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from C6- Cio aralkyl groups substituted by at least an halogen group, in particular -F;
or
❖ R2 represents a group selected from C6-Cio aryl and Cs-Cio-membered heteroaryl groups, said Ce-Cio aryl and C5-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
or
❖ Z represents a group Zl , R and R' being in particular different from H.
In a particular embodiment, Rj represents H.
In a particular embodiment, R4 represents H.
In a particular embodiment, R represents a benzyl group, optionally substituted as defined above, in particular by at least one halogen, more particularly F.
In an even more particular embodiment, R3 represents a benzyl, substituted by at least one halogen, more particularly F. In this case, R3 may represent a benzyl, substituted one halogen, more particularly F, or a benzyl, substituted by two halogen that are identical or different, more particularly F and/or CI, the substitution being for example in 2,3; 2,5; 2,6 or 3,5.
For example, R is selected from the group consisting of:
Figure imgf000024_0001
In a particular embodiment, R2 represents a phenyl, optionally substituted as defined above,
R2 representing in particular a phenyl optionally substituted by at least a halogen, in particular F;
R2 being more particularly selected from the group consisting of:
Figure imgf000025_0001
.In a particular embodiment, Z is selected from Zl and Z2,
R, R', R" and R"' each independently representing in particular H or a group selected from:
- a Ct-C6 alkyl group;
a C3-C7 cycloalkyl group;
an halogen;
a -ORi group;
a CF3 group;
Z being more particularly selected from the group consisting of:
Figure imgf000025_0002
It is to be noted that the present invention includes the combination of all the embodiments listed hereabove for R], R2, R3, R4 and/or Z.
In particular, R3 represents a benzyl, optionally substituted by at least one halogen, more particularly F. and Z represents a furanyl, or thiophenyi, optionally substituted by at least one Yz as defined above.
In a particular embodiment, the compound of formula (III) is a compound of following formula (Ilia):
Figure imgf000026_0001
wherein:
R. being in particular selected from the group consisting of:
Figure imgf000026_0002
R- being in particular selected from the group consisting of:
Figure imgf000026_0003
Z is selected from the group consisting of:
Figure imgf000026_0004
In a particular embodiment, the present invention relates to compounds with the general formula Ilia and the following names:
8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7/ )-one
8-benzyl-2-((4,5-dimethyli iran-2-yl)methyl)-6-phenylimidazo[i,2-a]pyrazin-3(7/ )-one 8-benzyl-6-(2-fluorophenyl)-2-(furan-2-ylmethyl)iraidazo[l ,2-a]pyrazin-3(7//)-one
8-benzyl-2-(3-methylbenzyl)-6-phenylimidazo[l ,2- ]pyrazin-3(7/ )-one 8-b€nzyl-2-(3-methoxybenzyl)-6-phenylimidazo[l,2-«]pyrazin-3(7 )-one
2,8-dibcnzyl-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3(7H)-one
8-benzyl-6-(2,6-difluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-a]pyrazin-3(7 -°ne 8-benzyl-6-phenyl-2-((5-(trifluoromethyl)f^
2,8-dibenzyl-6-(2,6-difluorophenyl)imidazo[ 1 ,2- ]pyrazin-3(7H)-one
8-benzyl-6-(2-fluorophenyl)-2-((5-methylftiran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3(7/T -one 8-benzyl-2-((5-cyclopropylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one 8-benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3(7H)-one 8-benzyl-6-(3-fluorophenyl)-2-((5-mcthylfuran-2-yl)methyl)imidazo[l,2-a]pyrazin-3(7 /)-one 8-benzyl-2-(2-fluorobcnzyl)-6-phenylimidazo[l ,2-o]pyrazin-3(7H)-one
8-benzyl-2-((5-cthylthiophen-2-yl)methyl)-6-phenylimidazo[l,2- ]pyrazin-3(7/ )-one 8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin- 3(7H)-one
2-benzyl-8-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
2-benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one
8-(3-fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2- ]pyrazin-3(7 )-one
8-(2-fluorobenzyl)-2-(3-mcthylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one
8-(2-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3 (7//)-one
8-(2-fluorobenzyl)-2-(iijran-2-yhnethyl)-6-phenylirnidazoi l ,2-a]pyrazin-3(7H)-one
8-(2-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one 8-(3-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3(7/ )-one
8-(3-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[l,2-ii]pyrazin-3(7H)-one
2-((5-ethylfiiran-2-yI)methyl)-8-(3-fluorobenzyl)^^ . 8-(2-chlorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)- one
8-(3-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-fl]pyrazin-3(7H)-one 2-((5-ethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[l,2-«]pyrazin-3(7H)-one 8-(3-fluorobenzyl)-6-(2-fluorophenyl)-2-((5-methylftiran-2-yl)methyl)imidazo[l,2-a]pyrazin- 3(7//)-one
8-(2,3-difluorobenzyl)-2-(ftiran-2-ylmethyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one
8-(2,3-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)- one
2-benzyl-8-(2,3-difluorobenzyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one 8-(2,6-Difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin- 3(7//)-one
8-(2,3-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin- 3(7H)-one
8-(2,3-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7/ )- one
8-(2,6-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]p^
one
2-((4,5-Dimethylforan-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimida
3(7//)-one
2-((4,5-Dimethylmran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin- 3(7H)-one
8-(2,3-difluorobenzyl)-2-((4-ethyl-5-methylmran-2-yl)methyl)-6-phenylimidazo[l,2- a]pyrazin-3 (7H)-one
8-(23-difluorobenzyl)-2-((5-ethyl-4-methyliuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2- a]pyrazin-3 (7/ )-one
8-benzyl-2-(furan-2-ylmethyl)-6-(3-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3(7H)-one.
The present invention also relates to the use of a compound of formula (III) or (Ilia) as defined above as an in vitro or ex vivo diagnostic tool.
The present invention also relates to a compound of formula (III) or (Ilia) as defined above for use as an in vivo diagnostic tool.
The present invention also relates to a method for producing luminescence, which comprises contacting a compound of formula (III) or (Ilia) as defined above with a luminogenic protein, in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
The present invention also relates to a method of detecting luminescence in a sample comprising
contacting a sample with a compound of formula (III) or (Ilia) as defined above;
- optionally contacting the sample with a luminogenic protein, in particular an
Oplophonis luciferase, more particularly the nanoKAZ luciferase, if not present in the sample; and
detecting luminescence.
In a particular embodiment, the sample contains live cells. In another particular embodiment, the sample contains a luminogenic protein, in particular an Oplophoras luciferase, more particularly the nanoKAZ luciferase.
The present invention also relates to a method for detecting luminescence in a transgenic animal expressing a luminogenic protein, comprising:
administrating a compound of formula (III) or (Ilia) as defined above to said animal; and
detecting luminescence.
The present invention also relates to a method for assaying the activity of a sequence associated with promoter regulation, which comprises using a polynucleotide encoding a luminogenic protein as a reporter gene and, as a luminescence substrate, a compound of formula (III) or (Ilia) as defined above.
In another aspect, the present invention relates to a compound of following formula
(IV):
Figure imgf000029_0001
wherein :
Ri, R.2, R.3, R4 and Z are as defined for formula (III) or (Ilia).
R5 is as defined above.
It is to be noted that the present invention includes the combination of all the embodiments listed hereabove for formula (III) and/or (Ilia).
In another aspect, the present invention relates to a compound of following formula
(V):
Figure imgf000029_0002
wherein Rt , R2, R3, R4 and Z are as defined for formula (III) or (Ilia).
It is to be noted that the present invention includes the combination of all the embodiments listed hereabove for formula (III) and/or (Ilia).
In another aspect, the present invention relates to a compound of following formula
(VI):
Figure imgf000030_0001
wherei :
R], R2, R3, RA and Z are as defined for formula (III) or (Ilia);
Rd represents a group selected from Ci-Cg alkyl, C3-C cycloalkyl and aralkyl, in particular, methyl, ethyl, or benzyl.
It is to be noted that the present invention includes the combination of all the embodiments listed hereabove for formula (III) and/or (Ilia).
In a particular embodiment, the present invention relates to compounds with the general formula (IV) and the following names:
8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-«]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-«)pyrazin-3-yl acetate 8-benzyl-6-(2-fluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-fl]pyrazin-3-yl acetate
8-benzyl-2-(3-methylbenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2-fluorophenyl)imidazo[l ,2-aJpyrazin-3-yl acetate
8-benzyl-6-(2,6-diiluorophenyl)-2-(iuran-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-6-phenyl-2-((5-(trifluoromethyl)furan-2-yl)methyl)imidazo[ l ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2,6-difluorophenyl)imidazo[ 1 ,2-«]pyrazin-3-yl acetate
8-benzyl-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-cyclopropylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2- ]pyrazin-3-yl acetate 8-benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-6-(3-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyI-2-((4,5-dimethylfijran-2-yl)methyl)-6-(2-fluorophCTyl)imidazo[l,2-a]pyrazi acetate
2-benzyl-8-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-«]pyrazin-3-yl acetate
2-benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-o]pyrazin-3-yl acetate
8-(3-fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yI acetate
8-(2-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylirnidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-c]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-(3-f!uorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-((5-ethylfuran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2-chlorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(3-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-rt]pyrazin-3-yl acetate
2- ((5-ethylftjran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-«]pyrazin-3-yl acetate 8-(2,6-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3,5-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-chlorobenzyl)-2-(furan-2-ylmethyl)-6-phenyIimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-
3- yl acetate
8-(2,3-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,5-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,5-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-(3,5-difluorobenzyl)-2-((5-methyli iran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-benzyl-8-(2,3-difluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(2-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(3-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 2-benzyl-6-phenyl-8-(2,3,5-trifluorobenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,6-DifluQrobenzyl)-2-((5-methylfuran-2-yl)m
acetate
8-(2,5-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3- yl acetate
8-(2,5 )ifluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,3-Difluorobenzyl)-2-((4,5-dimethyliuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin yl acetate
8-(3,5-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ l >2-a]pyrazin-3- yl acetate
2-(furan-2-ylmethyl)-6-phenyl-8-(2,3,5-trifluorobenzyl)imidazo[l ,2-a]p Tazin-3-yl acetate 8-(2,3-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-(3,5-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((5-ethylfiiran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3- yl acetate
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(2-fluorobenzyr)-6-phenylimidazo[l ,2-a]pyrazin-3 acetate
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-Benzyl-6-phenyl-2-((5-propylfuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,3-difluorobenzyl)-2-((4-ethyl-5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2- a]pyrazin-3-yl acetate
8-benzyl-2-((4-ethyl-5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((5-isopropyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethyl-4-methylftiran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(2,3-difluorobenzyl)-2-((5-ethyl-4-methyliliran-2-yl)methyl)-6-phenylirnidazo[l ,2- a]pyrazin-3-yl acetate
8-benzyl-2-(l -(furan-2-yl)ethyl)-6-phenylimidazo[ l ,2-a]pyrazin-3 -yl acetate 8-(2,3-difluorobenzyl)-2-((5-isopropyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2- a]pyrazin-3-yl acetate
8-benzyl-2-(furan-2-ylmethyl)-6-(3-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate; or compounds with the general formula (V) and the following names:
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4,5-dimethylfuran-2-yl)propanoic acid 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(ftiran-2-yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoic acid
(3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)phenylalanine
2-((3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-(trifiuoromethyl)iuran-2-yl)propanoic acid (3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-yl)phenylalanine
2-((3-benzyl-5-(2-fluorophenyl)p>Tazin-2-yl)amino)-3-(5-methylruran-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-cyclopropylfuran-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-fluorophenyl)propanoic acid
2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoic acid 2-((3-benzyl-5-(3-fluorophenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(2-fluorophenyl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethylthiophen-2-yl)propanoic acid
2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(4,5-dimethylfuran-2-yl)propanoic acid
(3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)phenylalanine
(3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)phenylalanine
2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoic acid 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoic acid
2- ((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
3- (5-ethylfuran-2-yl)-2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)propanoic acid 2-((3-(2-chlorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfiiran-2-yl)propanoic acid 2- ((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid
3- (5-ethyl uran-2-yl)-2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)propanoic acid 3-(3-(2,6-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(3,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(iuran-2-ylmcthyl)propanoic acid
3 -(3 -(3 -chlorobenzyl)-5 -phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(3-fluorobenzyl)-5-(2-iluorophenyl)pyrazin-2-yl)-2-((5-methylfuran-2- yl)methyl)propanoic acid
3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(2,3-difluorobenzyl)-5-phenylp>Tazin-2-yl)-2-((5-methylfiiran-2-yl)methyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
3-(3-(3,5-difluorobenzyl)-5-phenylp>Tazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
2-benzyl-3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)propanoic acid
2-benzyl-3-(5-phenyl-3-(2-(trifluoromethyl)benzyl)pyrazin-2-yl)propanoic acid
2-benzyl-3 - (5 -phenyl-3 -(3 -(trifluoromethyl)benzyl)pyrazin-2-yl)propanoic acid
2- benzyl-3-(5-phenyl-3-(2,3,5-trifluorobenzyl)pyrazin-2-yl)propanoic acid
3-(3-(2,6-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
3- (3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)raethyl)propanoic acid 3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((4,5-dimethylfliran-2-yl)rnethyl)propanoic acid
3-(3-(3,5-difluorobenzyl)-5-phenylp Tazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid
3-(furan-2-yl)-2-((5-phenyl-3-(2,3,5-trifluorobenzyl)pyrazin-2-yl)methyl)propanoic acid 3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)methyl)propanoic acid 3-(3-(3,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)methyl)propanoic acid 3-(3-(2,6-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)methyl)propanoic acid 3-(3-(2,6-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid 3-(4,5-dimethylfuran-2-yl)-2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)methyl)propanoic acid
3-(4,5-dimethyliuran-2-yl)-2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)methyl)propanoic acid
2-((3-(2,3 -difluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3 -(4-ethyl-5 -methyl furan-2- yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-ethyl-5-methylfuran-2-yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-isopropyl-4-methylfuran-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethyl-4-methyl uran-2-yl)propanoic acid 2-((3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-ethyl-4-methylfuran-2- yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)butanoic acid (144)
2-((3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-isopropyl-4-methylfuran-2- yl)propanoic acid
2-((3-benzyl-5-(3-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid 2-((3-benzyl-5-(3-(hydroxy)phenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid.
In another aspect, the present invention relates to a compound of following formula
(VII):
Figure imgf000035_0001
wherein :
Ri represents H or a group selected from Ci-C6 alkyl, C3-C7 cycloalkyl, C6-Cio aryl, aralkyl and C5-C 10-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, C6-C]0 aryl, aralkyl and C5-Cio-membered heteroaryl groups being optionally substituted by at least one Yi group;
R2 represents a group selected from C6-Ci0 aryl and Cs-Cio-membered heteroaryl groups, said C6-Ci0 aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
R3 represents an aralkyl group, said aralkyl group being optionally substituted by at least one Y3 group;
Y is a leaving group, in particular an halogen, more particularly a -CI;
said Yi, Y2 and Y3 groups being each independently selected from:
a Ci-Cf, alkyl group; a C3-C7 cycloalkyl group;
an halogen;
a -ORii group;
Rii represents H, a Ci-C6 alkyl group, a C3-C 10 cycloalkyl group or an aralkyl with the proviso that said compound is not of one of the following formulae:
Figure imgf000036_0001
In a particular embodiment, Ri represents H.
In a particular embodiment, R3 represents a benzyl group, optionally substituted as defined above, in particular by at least one halogen, more particularly F,
R being more particularly selected from the group consisting of:
Figure imgf000036_0002
In a particular embodiment, R2 represents a phenyl, optionally substituted as defined above,
R2 representing in particular a phenyl optionally substituted by at least an halogen, in particular F;
R being more particularly selected from the group consisting of:
Figure imgf000036_0003
Definitions
The following terms and expressions contained herein are defined as follows:
As used herein, a range of values in the form "x-y" or "x to y", or "x through y", include integers x, y, and the integers therebetween. For example, the phrases "1 -6", or "1 to 6" or "1 through 6" are intended to include the integers 1, 2, 3, 4, 5, and 6. Preferred embodiments include each individual integer in the range, as well as any subcombination of integers. For example, preferred integers for "1-6" can include 1, 2, 3, 4, 5, 6, 1-2, 1-3, 1-4, 1- 5, 2-3, 2-4, 2-5, 2-6, etc.
As used herein, the term "alkyl" refers to a straight-chain, or branched alkyl group having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec- butyl, tert-butyl, pentyl, isoamyl, neopentyl, 1 -ethylpropyl, 3-methylpentyl, 2,2- dimethylbutyl, 2,3-dimethylbutyl, hexyl, etc. The alkyl moiety of alkyl-containing groups, such as aralkyl or O-alkyl groups, has the same meaning as alkyl defined above. Lower alkyl groups, which are preferred, are alkyl groups as defined above which contain 1 to 4 carbons. A designation such as "C1-C4 alkyl" refers to an alkyl radical containing from 1 to 4 carbon atoms.
As used herein, the term "cycloalkyl" refers to a saturated or partially saturated mono- or bicyclic alkyl ring system containing 3 to 7 carbon atoms. A designation such as "C5-C6 cycloalkyl" refers to a cycloalkyl radical containing from 5 to 6 ring carbon atoms. Examples of cycloalkyl groups include such groups as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, pinenyl, and adamantanyl. The cycloalkyl moiety of cycloalkyl- containing groups, such as O-cycloalkyl groups, has the same meaning as alkyl defined above.
As used herein, the term "aryl" refers to a substituted or unsubstituted, mono- or bicyclic hydrocarbon aromatic ring system having 6 to 10 ring carbon atoms. Examples include phenyl and naphthyl. Preferred aryl groups include unsubstituted or substituted phenyl and naphthyl groups. Included within the definition of "aryl" are fused ring systems, including, for example, ring systems in which an aromatic ring is fused to a cycloalkyl ring. Examples of such fused ring systems include, for example, indane, indene, and tetrahydronaphthalene.
As used herein, the term "aralkyl" refers to a aryl alkyl group of formula Ar-alkyl-, wherein "aryl" and "alkyl" are as defined above, Ar being in particular Ph, alkyl being in particular Me (-CH2-).
As used herein, the term "heteroaryl" refers to an aromatic group containing 5 to 10 ring carbon atoms in which one or more ring carbon atoms are replaced by at least one hetero atom such as -0-, -N-, or -S-. Examples of heteroaryl groups include pyrrolyl, furanyl, thienyl, pirazolyl, imidazolyl, thiazolyl, isothiazolyl, isoxazolyl, oxazolyl, oxathiolyl, oxadiazolyl, triazolyl, oxatriazolyl, furazanyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, indolyl, isoindolyl, indazolyl, benzofuranyl, isobenzofuranyl, purinyl, quinazolinyl, quinolyl, isoquinolyl, benzoimidazolyl, benzothiazolyl, benzothiophenyl, thianaphthenyl, benzoxazolyl, benzisoxazolyl, cinnolinyl, phthalazinyl, naphthyridinyl, and quinoxalinyl. Included within the definition of "heteroaryl" are fused ring systems, including, for example, ring systems in which an aromatic ring is fused to a heterocycloalkyl ring. Examples of such fused ring systems include, for example, phthalamide, phthaiic anhydride, indoline, isoindoline, tetrahydroisoquinoline, chroman, isochroman, chromene, and isochromene.
As used herein, the term "hetaralkyl" refers to a heteroaryl alkyl group of formula Heteroaryl-alkyl-, wherein "heteroaryl" and "alkyl" are as defined above, alkyl being in particular Me (-CH2-).
As used herein, the term "pharmaceutically acceptable" refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem complications commensurate with a reasonable benefit/risk ratio.
As used herein, the term "luminogenic proteins" refers to luciferases and photoproteins using luciferins.
By "strong acid" is meant an acid that ionizes completely in aqueous solution by losing one proton.
By "leaving group" is meant a nucleofuge which retains the lone pair from its previous bond with another species, in particular in a nucleophilic substitution reaction. The leaving group is for example chosen from the group consisting of halogens, in particular -I, -Br, -CI and -F, and triflate -OS(02)CF3.
All other terms used in the description of the present invention have their meanings as is well known in the art.
In another aspect, the present invention is directed to pharmaceutically acceptable salts of the compounds described above. As used herein, "pharmaceutically acceptable salts" includes salts of compounds of the present invention derived from the combination of such compounds with non-toxic acid.
Acid addition salts include inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulfuric, nitric and phosphoric acid, as well as organic acids such as acetic, citric, propionic, tartaric, glutamic, salicylic, oxalic, methanesulfonic, para-toluenesulfonic, succinic, and benzoic acid, and related inorganic and organic acids.
In addition to pharmaceutically-acceptable salts, other salts are included in the invention. They may serve as intermediates in the purification of the compounds, in the preparation of other salts, or in the identification and characterization of the compounds or intermediates.
The pharmaceutically acceptable salts of compounds of the present invention can also exist as various solvates, such as with water, methanol, ethanol, dimethyl formamide, ethyl acetate and the like. Mixtures of such solvates can also be prepared. The source of such solvate can be from the solvent of crystallization, inherent in the solvent of preparation or crystallization, or adventitious to such solvent. Such solvates are within the scope of the present invention.
It is recognized that compounds of the present invention may exist in various stereoisomeric forms. As such, the compounds of the present invention include both diastereomers and enantiomers. The compounds are normally prepared as racemates and can conveniently be used as such, but individual enantiomers can be isolated or synthesized by conventional techniques if so desired. Such racemates and individual enantiomers and mixtures thereof form part of the present invention.
It is well known in the art how to prepare and isolate such optically active forms.
Specific stereoisomers can be prepared by stereospecific synthesis using enantiomerically pure or enantiomerically enriched starting materials. The specific stereoisomers of either starting materials or products can be resolved and recovered by techniques known in the art, such as resolution of racemic forms, normal, reverse-phase, and chiral chromatography, recrystallization, enzymatic resolution, or fractional recrystallization of addition salts formed by reagents used for that purpose. Useful methods of resolving and recovering specific stereoisomers described in Eliel, E. L.; Wilen, S.H. Stereochemistry of Organic Compounds; Wiley: New York, 1994, and Jacques, J, et al. Enantiomers, Racemates, and Resolutions; Wiley: New York, 1981 , each incorporated by reference herein in their entireties.
Synthesis
The compounds of the present invention may be prepared in a number of methods well known to those skilled in the art, including, but not limited to those described below, or through modifications of these methods by applying standard techniques known to those skilled in the art of organic synthesis. The appropriate modifications and substitutions will be readily apparent and well known or readily obtainable from the scientific literature to those skilled in the art. In particular, such methods can be found in R.C. Larock, Comprehensive Organic Transformations, Wiley-VCH Publishers, 1999. All processes disclosed in association with the present invention are contemplated to be practiced on any scale, including milligram, gram, multigram, kilogram, multikilogram or commercial industrial scale.
It will be appreciated that the compounds of the present invention may contain one or more asymmetrically substituted carbon atoms, and may be isolated in optically active or racemic forms. Thus, all chiral, diastereomeric, racemic forms, isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated. It is well-known in the art how to prepare and isolate such optically active forms. For example, mixtures of stereoisomers may be separated by standard techniques including, but not limited to, resolution of racemic forms, normal, reverse-phase, and chiral chromatography, preferential salt formation, recrystallization, and the like, or by chiral synthesis either from chiral starting materials or by deliberate synthesis of target chiral centers.
Compounds of the present invention may be prepared by a variety of synthetic routes. The reagents and starting materials are commercially available, or readily synthesized by well-known techniques by one of ordinary skill in the arts. All substituents, unless otherwise indicated, are as previously defined.
In the reactions described hereinafter, it may be necessary to protect reactive functional groups, for example hydroxy, amino, imino, thio or carboxy groups, where these are desired in the final product, to avoid their unwanted participation in the reactions. Conventional protecting groups may be used in accordance with standard practice, for examples see T.W. Greene and P. G. M. Wuts in Protective Groups in Organic Chemistry, 3rd ed., John Wiley and Sons, 1999; J. F. W. McOmie in Protective Groups in Organic Chemistry, Plenum Press, 1973.
The present invention also relates to a process of preparation of a compound of formula (III) as defined above, comprising a step of conversion of a compound of formula (IV) as defined above into a compound of formula (III).
In a particular embodiment, the process of preparation of a compound of formula (III) as defined above comprises:
i) A step of contacting a compound of formula (IV) as defined above with a strong acid in presence of a solvent of the compound of formula (IV); or
ii) A step of contacting a compound of formula (IV) as defined above with a strong acid or ammonia, in particular under inert gas when ammonia is used, in presence of a solvent of the compound of formula (IV).
In a particular embodiment, the strong acid is hydrochloric acid. In a particular embodiment, the conversion of (IV) into (III) is conducted at a temperature comprised from 40°C to 90°C, for example at 50°C, in particular in a sealed tube.
In a particular embodiment, the strong acid used for the conversion of (IV) into (III) is hydrochloric acid, in particular in solution in ethanol.
In a particular embodiment, the conversion of (IV) into (III) is conducted at a temperature comprised from 40°C to 90°C, in particular at 50°C.
In a particular embodiment said compound of formula (TV) is prepared according to a process comprising:
i) A step of conversion of a compound of formula (VI) as defined above into a compound of formula (V), followed by a step of conversion of the compound of formula (V) into a compound of formula (IV); or
ii) A step of conversion of a compound of formula (VI) into a compound of formula (IV).
In a particular embodiment said compound of formula (IV) is prepared according to a process comprising:
i) A step of hydrolysis of the COO-Ra ester function of a compound of formula (VI) as defined above, in particular by contacting the compound of formula (VI) with a strong base in presence of a solvent of said compound of formula (VI) to give a compound of formula (V), followed by a step of contacting the compound of formula (V) with a compound selected from the group consisting of anhydrides, chlorofomates and dicarbonates; or
ii) A step of contacting said compound of formula (VI) with a mixture of acetic acid and aliphatic anhydride, in particular acetic anhydride.
In a particular embodiment, the strong base used for the conversion of (VI) into (V) is sodium hydroxide, the hydrolysis being in particular conducted at a temperature comprised from 15 to 30°C.
In a particular embodiment, the compound selected from the group consisting of anhydrides, chlorofomates and dicarbonates is acetic or pivalic anhydride, the contacting with said compound being in particular conducted at a temperature comprised from 15 to 30°C.
In a particular embodiment, the conversion of (VI) into (IV) is conducted at a temperature comprised from 100°C to 150°C, in particular at 140°C, in particular under microwave irradiation.
In a particular embodiment, said compound of formula (VI) is obtained from a process comprising: i) a step of replacement of the hydroxyl group of a compound of following formula (VIII):
Figure imgf000042_0001
by a leaving group -Y, Y being in particular an halogen, to give a compound of following formula (VII):
Figure imgf000042_0002
and
ii) a step of contacting the compound of following formula (VII):
with a compound of following formula (IX):
Figure imgf000042_0003
(IX)
wherein
Ri , R2, R3, R4, Z and Rd are as defined above,
in presence of a catalyst comprising copper or palladium and a ligand chosen from BINAP, Xantphos, dichloro[ l ,3-bis(2,6-di-3-pentylphenyl)imidazol-2-yIidene](3- chloropyridyl) (PEPPSI-IPentCl),
thereby obtaining a compound of formula (VI).
In a particular embodiment, Y is CI, step vi) being a step of contacting a compound of formula (VIII) with phenylphosphonic dichloride, in particular at a temperature comprised from 60 °C and 150 °C, more particularly at 100°C.
In a particular embodiment, Y is Br, step i) being a step of contacting a compound of formula (VIII) with triflic anhydride, followed by a step of contacting the product obtained in previous step with sodium bromide, in particular at a temperature comprised from 80°C and 160°C, for example at 120°C, in particular in a sealed tube.
In a particular embodiment, step ii) is performed in presence of a base, in particular cesium carbonate.
In a particular embodiment, the catalyst and the ligand of step ii) are palladium acetate and BINAP, respectively, said step ii) being conducted at a temperature comprised from 40°C and 80°C, more particularly at 60°C In a particular embodiment, said compound of formula (VIII) is obtained from a process comprising a step of dehydrogenation and aromatization of a compound of following formula (X):
Figure imgf000043_0001
In particular from a process comprising:
i) A step of contacting the compound of formula (X) with sulfur, in presence of a solvent, at a temperature comprised from 180 °C and reflux; or
ii) A step of contacting the compound of formula (X) with peroxide-based oxidants such as peracetic acid, followed by a step of thermal treatment at a temperature comprised from 150°C to 190°C, in particular in presence of a solvent.
The general routes to prepare the examples of the present invention are shown in the schemes A-C hereafter.
The reagents and starting materials are commercially available, or readily synthesized by well-known techniques by one of ordinary skill in the arts. All the substituents of compounds A-Q depicted in schemes A-C are, unless otherwise indicated, as previously defined above for the corresponding compounds (III)-(X).
Figure imgf000043_0002
R' N OTf Step I RI N x Step m R1 N NH2
R2 N R3
L / (VII)
(X = halogen)
Scheme A
Concerning the synthetic pathways described in scheme A, step a is a 1 ,4-addition of aminoesters B on nitrovinyls A which is preferably achieved in the absence of solvents. Step b is the reduction of the nitro group of compounds C which is preferably achieved in acidic medium using zinc. Step c involves the heat-triggered cyclization of the resulting free diamine D into compound E. Steps a, b and c were usually telescoped in order to minimize side reactions and potential losses in the course of purification processes. Step e is a dehydrogenation/aromatization of compound E to give the hydroxypyrazine derivatives H, which is preferably achieved using sulfur in a boiling inert solvent. An alternative was found via the preparation of 5-oxo-2,3,4,5-tetrahydropyrazine 1 -oxide derivatives J using peracetic acid (step f) and its rearrangement into hydroxypyrazine derivatives II (step h). When R1 is H, the 3 -hydroxypyrazine H can also be prepared, via step g, using the reported (J. Am. Chem. Soc, 1949, 78; US 2520088, 1949; J. Am. Chem. Soc, 1952, 1580; US 4046763, 1977) condensation of glyoxals G and aminoamides F (made from the reaction between aminoesters B and ammonia via step d). Step j is the replacement of the hydroxyl group of compound H by a halogen to give compounds L. In the case of the group X being a chloride, the preferable reagent used to achieve this transformation is phenylphosphonic dichloride. These compounds can also be prepared in two steps (i and 1) via an ester intermediate endowed with a strong leaving capacity such as the triflic esters K followed by a reaction akin to an aromatic Finkelstein reaction (Heterocycles, 1998, 1255; WO 20121 19046, 2012). A subsequent substitution with an iodine salt to obtain iodo derivatives (X = I) is also possible via known acid-catalysed reaction (J. Org. Chem., 1961, 1907; Tetrahedron, 1998, 9701 ; Synlett, 2003, 1801). Moreover, the direct conversion of the of 5-oxo-2,3,4,5-tetrahydropyrazine 1 -oxide derivatives J into chloropyrazines L (step k) was also found possible for instance by using phenylphosphonic dichloride and heat. Finally, a recent publication (Org. Process. Res. Dev. 2017, 21 , 346) is offering the possibility to prepare, from these halogenopyrazines, the corresponding aminopyrazines M, via step m, which are key intermediates in the vast majority of the previously reported preparation of imidazo[l ,2-a]pyrazine-3(7H)-one luciferins (Chem. Eur. J. 2015, 21 , 17158).
As depicted in scheme B, from halogenopyrazines L, the preparation of the O- protected luciferins with the general formula P was found to be possible, either in two separated steps via the amino acids O or in one pot from their amino esters precursors with the general formula N. Amongst many interesting aspects, it is important to mention that our invention is answering the unmet need of disposing of a fresh solution, and thus of a reproducible titre, of any luciferin with the general formula Q. Since the 0-protected luciferins (pre-luciferins), with the general formula P, can upon a simple and unprecedented acid treatment, lead to ready-to-use solutions of the corresponding luciferins Q. Indeed, these 0-protected luciferins P are much more stable and can be conveniently shipped and stored for a long time at room temperature and then hydrolysed to readily generate solutions of luciferins for their immediate use. Moreover, the removal of such group is simply achieved in a vial using solutions containing a strong acid, such as hydrochloric acid, without the need of an inert atmosphere. This unprecedented use of an acid is actually instrumental in providing a degree of stability of the resulting solution as little or no product resulting from the chemoluminescence process is observed in the course of this deprotection step. This is in stark contrast with previously reported {Tetrahedron Lett., 1977, 2685; Chemistry, 2013, 14970) uses of a base, such as ammonia, which in the presence of oxygen does lead to much more extensive decomposition. Concerning the chemistry described in scheme B, Step n is an amination reaction using a wide variety of a-amino esters B to give compounds N. This can be achieved using copper or palladium catalysts and a variety of ligands as described in the case of many halogenated aromatic or heteroaromatic derivatives (Acc. Chem. Res., 1998, 852; J. Organomet. Chem., 1999, 125; Chem. Rev., 2006, 2651 ; Angew. Chem., Int. Ed., 2008, 6338; Angew. Chem., Int. Ed., 2008, 3096; J. Org. Chem., 2013, 7930; Org. Lett., 2016, 4128; Beilstein J. Org. Chem., 2012, 2004; Tetrahedron, 2011, 9405). In the present cases, in order to avoid side reactions, this step was found to require a low (60 °C) temperature. Step o is a hydrolysis of the ester function of N, preferably achieved under basic conditions to give the acids O. Step p is a cyclization followed by an O-protection which is achieved by activation of the acid function of O, preferably with a variety of reagents such as anhydrides, chloroformates, or dicarbonates. This gives directly the rather more stable O-protected compounds P. Moreover, the step o and p can in many cases be performed in one pot (step q) using acetic acid along with acetic anhydride. Finally, the compounds N or O can be transformed directly into the corresponding luciferin Q using for instance hot acetic acid and/or hydrochloric acid (step r).
Step q
Figure imgf000046_0001
Q / (HI). (iHa)
Scheme B
When considering each synthetic steps individually depicted in the schemes above, step e, the dehydrogenation aromatization of piperazin-2-ones E into 2-hydroxypyrazines H, the preparation of the N-oxides J via step f and their rearrangement into 2-hydroxypyrazines H via step h , the transformation of the JV-oxides J into the halogenopyrazines L via step k and the isolation of the O-protected derivatives P obtained from compounds O via step p or from compound N via step q are the only one which have no precedents. Moreover, before or after each of these steps, further chemistry focusing on the side chains R', R2, R3, and R4 and their various protecting groups or functions can be planned in order to alter them and/or make them compatible with the chemistry used in the next steps. For instance, the hydrogenation of eventual benzyl groups protecting OH functions present on compounds of type N or O can be undertaken before their transformations into compounds of type P via step o-q.
These synthetic pathways are making extensive uses of many aminoesters of type B. Accordingly, to prepare such intermediates; we used for instance the pathways depicted in scheme C. The first path is based on the reported (Bull. Acad. Sci. USSR Div. Chem. Sci. (Engl. Transl), 1980, 458; Bull. Acad. Sci. USSR Div. Chem. Sci. (Engl. Tmnsl), 1981, 466) use of ethyl nitroacetate to prepare the corresponding nitroacrylates which are then reduced into aminoesters. A simplification of the procedure led us to telescope the first three steps of this method and only properly isolate the intermediate nitroesters R before their final reduction into aminoesters B. The second path is endowed with a larger scope and is based on the use of a Knoevenagel reaction with diethylmalonate which, after a reduction, gives the corresponding substituted malonates. From them, as reported (Ber. Dtsch. Chem. Ges. , 1902, 3772; Org. Process. Res. Dev., 2007, 1069), a-oxime esters are readily prepared and can be reduced into the corresponding aminoesters. Again, telescoping the first three steps very often led us to properly isolate only the intermediate oximes S before their final reduction into aminoesters. As further described in detail below, less general methods, were also employed to prepare other types -oxime esters.
Figure imgf000047_0001
R B / (IX)
-C02Et v D/^C02E! Hi or vi R/ 02Et vii R-^y >-QH iv ρΤ~γΝΗ2
R C02Et C02Et C02Et C02Et C02Et
S B / (IX)
Scheme C. i: HC(OMe)3( Dowex SOW, MeOH. ii: Ac20, heat, iii: NaBHL,, iPrOH. iv: Zn, EtOH, H30+CF. v: AcOH, piperidine, EtOH, 4A molecular sieve, vi: H2, Pd/C, EtOH. EtONa, vii: a) EtOH, isoamylnitrite, b) H30+C!\ DRAWINGS
Figure 1 illustrates the HPLC profiles over time for the acetyl hydrolysis of P-21 ( = 451) into the corresponding luciferin Q-12 (M = 409). Obtained on an Agilent apparatus, with a 3.5 μηι XDB-C18 column and a water/methanol gradient (containing 5 mM ammonium formate, from 95-5 to 5-95 in 3.5 min, 3.5 min at 5-95, and then back at 95-5 in 0.5 min), UV monitoring set at 254 n. .
Figure 2 illustrates the bioluminescence emission over two hours of furimazine (Q-l) and hydroxyfurimazine (Q-80), as well as the imidazo[l,2- ]pyrazin-3(7H)-ones Q-84 and Q-85 of the invention.
Figure 3 illustrates dynamic range (relative units (RLU) versus serial dilutions) of nano AZ, which was plotted using the imidazof l ,2- ]pyrazin-3(7//)-one Q-3.
Figure 4 shows a comparative Lineweaver-Burk representation of the bioluminescent activity of the nanoKAZ using furimazine (Q-l) and imidazo[ 1 ,2-a]pyrazin-3(7H)-one Q-3 as substrates: the inverse of the reaction rate (relative units [RLU] / second) is plotted versus the inverse of the substrate concentration (105Μ~'), keeping constant the concentration of the enzyme in the both cases. The two dotted lines (linear regression) are converging to the same intercept with the x-axis (-1/Km) and different intercepts with the y-axis (1/Vmax). The equations of the two linear regressions are indicated and the corresponding correlation factors (R2) with the experimental data are reported.
Figure 5 compares expression and location of protein chimeras fused to the nanoKAZ in transiently transfected HEK and Jurkat cell lines, and primary human T-cells: the cytokine receptor IL7Ra:nanoKAZ at the membrane (top), the enconsin tubulin binding protein
EMTB:nano AZ associated with microtubules (middle), the transcription factor STAT5a:nanoKAZ dense throughout the cytoplasm and faint in the nucleus (bottom). Images were acquired for one second from live cells using Q-l (top line) and Q-3 (lines 2-4) substrates showing the subcellular resolution in cell culture conditions and the gain of light emission with Q-3.
Figure 6 depicts a superposition of two Ή NMR spectra of the same batch of compound 8- benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate (stored at 4 °C) made on the day of its production (A) and 30 months after (B).
EXAMPLES
Description, chemistry
General experimental procedure
A Biotage initiator 2 microwave oven was used for reactions mentioning such heating method. !H NMR spectra were recorded on a Bruker Avance 400 spectrometer at 400 MHz and 100 MHz, respectively. Shifts (<5) are given in ppm with respect to the TMS signal and coupling constants (J) are given in Hertz. Column chromatography were performed either on Merck silica gel 60 (0.035 - 0.070 mm) or neutral alumina using a solvent pump and an automated collecting system driven by a UV detector set to 254 nm unless required otherwise. Sample deposition was carried out by absorption of the mixture to be purified on a small amount of the solid phase followed by its deposition of the top of the column. The low resolution mass spectra were obtained on an Agilent 1100 series LC/MSD system using an atmospheric electrospray ionization system and the high resolution mass spectra (HRMS) were obtained using a Waters Micromass Q-Tof with an electrospray ion source.
General procedures for the synthesis of a-aminoesters B via a-nitroesters R, preparation of nitroesters R. Step 1 : preparation of the dimethylacetal. The corresponding aldehyde (0.044 mol) and trimethylorthoformate (5.8 mL, 0.053 mol) were dissolved in methanol (7.3 mL, 0.24 mol, dried over 3A molecular sieve). To this was added the DOWEX 50VVX8-100 ion-exchange resin (0.2 g) and the solution was stirred overnight under a calcium chloride- protected atmosphere. The resin was then removed by filtration, the filter washed with dry methanol and the filtrate concentrated to dryness (at 30 mbar) to yield the corresponding volatile acetal (usually not fully stable in CDCI3). Step 2: condensation with ethyl nitroacetate, preparation of the nitroacrylates. In order to remove some eventual traces of water, prior to this reaction, under a calcium chloride-protected atmosphere, the nitroacetate (5.7 g, 0.042 mol) was stirred in acetic anhydride (5 mL, 0.053 mol) for 15 minutes. To this solution was added the crude acetal described above dissolved in acetic anhydride (5.1 mL, 0.053 mol) and the solution was heated at the temperature and time described in each cases below while allowing the resulting low boiling methyl acetate to distil off. This was then thoroughly concentrated to dryness to yield the crude 3-aryl-2-nitroacrylates. Step 3: reduction with sodium borohydnde, preparation of the substituted nitroesters. The crude acrylate was dispersed dissolved in isopropanol (100 mL, dried over 4A molecular sieve). To this was added portion-wise sodium borohydride (2 g, 0.053 mol) and the suspension was heated up to reflux before allowing it to cool back to room temperature. Acetic acid was then cautiously added (3.3 mL, 0.053 mol) and the isopropanol was removed under vacuum. The crude residue was dispersed in water (100 mL) and 10 % hydrochloric acid (5.7 mL, 0.0057 mol) was added to help the hydrolysis of half reacted boron hydrides. The resulting solution was extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The residue was then purified as described in each case below to yield the corresponding a-nitroester R.
Ethyl 3-(4-fluorophenyl)-2-nitropropanoate (R-l): Obtained as an oil (2.72 g, 23 % from 4- fluorobenzaldehyde) after heating at 190 °C for 4h in the course of the condensation step and two chromatography over silica gel (cyclohexane - ethyl acetate 9/1) and (cyclohexane - dichloromethane 2/1). Ή NMR (CDC13): 7.21 (m, 2H), 7.02 (m, 2H), 5.32 (dd, 1H, J = 5.7, 9.4), 4.30 (m, 2H), 3.56 (dd, 1H, J = 9.4, 14.7), 3.47 (dd, 1H, J = 5.7, 14.7), 1.31 (m, 3H). HRMS (m/z) [M-H]' calcd for C, ,H, ,FN04: 240.0672; found, 240.0645.
Ethyl 3-(2-methoxyphenyl)-2-nitropropanoate (R-2): Obtained as an oil (6.12 g, 53 % from 2- methoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1). Ή NMR (CDCI3): 7.28 (m, 1H), 7.14 (m, III), 6.91 (m, 2H), 5.57 (dd, 1 H, J = 6.2, 9.1), 4.28 (q, 2H, J = 7.1), 3.82 (s, 3H), 3.55 (dd, 1H, J = 6.2, 14.1), 3.51 (dd, 1H, J= 9.1 , 14.1), 1.28 (t, 3H, J = 7.1). HRMS: calcd for Ci2H15N05Na: 276.0848; found, 276.0831.
Ethyl 3-(3-methoxyphenyl)-2-nitropropanoate (R-3): Obtained as an oil (2.34 g, 21 % from 3- mcthoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1). Ή NMR (CDCI3): 7.25 (t, 1H, J = 7.9), 6.81 (m, 3H), 5.35 (dd, III, J - 5.8, 9.3), 4.31 (q, 2H, J= 7.2), 3.81 (s, 3H), 3.56 (dd, 1H, J = 9.3, 14.7), 3.47 (dd, 1H, J = 5.8, 14.7), 1.31 (t, 3H, J = 7.2). HRMS: calcd for Ci2H,5N05Na: 276.0848; found, 276.0831. Ethyl 3-(4-methoxyphenyl)-2-nitropropanoate (R-4): Obtained as an oil (6.95 g, 55 % from 4- methoxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1), !H NMR (CDCI3): 7.15 (m, 211), 6.86 (m, 2H), 5.31 (dd, 1H, J = 5.9, 9.5), 4.30 (m, 2H), 3.81 (s, 3H), 3.52 (dd, 1H, J = 9.5, 14.6), 3.44 (dd, HI, J = 5.9, 14.6), 1.31 (t, 3H, J = 7.1). HRMS: calcd for C! 2H15N05Na: 276.0848; found, 276.0840.
Ethyl 3-(4-(benzyloxy)phenyl)-2-nitropropanoate (R-5): Obtained as an yellow solid (4.08 g,
51 % from 4-benzyloxybenzaldehyde) after heating at 150 °C for 9h in the course of the condensation step and a chromatography over silica gel (cyclohexane - ethyl acetate 92/8 to 9/1). Ή NMR (CDCI3): 7.46-7.33 (m, 5H), 7.15 (m, 2H), 6.94 (m, 2H), 5.31 (dd, 1H, J = 6.0, 9.3), 5.06 (s, 2H), 4.30 (m, 2H), 3.52 (dd, 1 H, J = 9.3, 14.6), 3.44 (dd, 1H, J= 6.0, 14.6), 1.30 (t, 3H, J = 7.1 ). HRMS: calcd for C18HI9N05Na: 352.1161; found, 352.1150.
Ethyl 2-nitro-3-m-tolylpropanoate (R-6): Obtained as an oil (1.05 g, 16 % from 3- methylbenzaldehyde) after heating at 180 °C for 8h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 2/1 to 1/6) and (cyclohexane ~~ ethyl acetate 97/3). Ή NMR (CDCI3): 7.22 (m, 1H), 7.60 (m, 1H), 7.01 (m, 2H), 5.33 (dd, 1H, J = 5.8, 9.4), 4.30 (q, 2H, J = 7.1), 3.54 (dd, 1H, J = 9.4, 14.6), 3.46 (dd, 1H, J = 5.8, 14.6), 2.34 (s, 3H), 1.30 (t, 3H, J = 7.1). HRMS (m/z): [M-H]" calcd for C12H14NO4: 236.0923; found, 236.0983.
Ethyl 3-(furan-2-yl)-2-nitropropanoate (R-7): In the present case, the nitroacrylate was prepared directly from furfural as previously described.(Og. Biomol. Chem., 2012, 524) Obtained as an oil (5.04 g, 39 % from furfural) after a chromatography over silica gel (dichloromethane - methanol 99/1). Ή NMR (CDCI3): 7.36 (m, 1H), 6.32 (m, 1H), 6.19 (m, 1H), 5.44 (dd, 1H, J = 5.5, 9.3), 4.32 (q, 2H, J = 7.2), 3.66 (dd, 1H, J - 9.3, 15.7), 3.54 (dd, 1H, J = 5.5, 15.7), 1.32 (t, 3H, J = 7.2). HRMS: calcd for C9HuN05 a: 236.0535; found, 236.0522.
Ethyl 3-(5-methylfuran-2-yi)-2-nitropropanoate (R-8): Obtained as an oil (3.44 g, 60 % from
5-methyl furfural) after heating at 140 °C for 2h in the course of the condensation step and a chromatography over silica gel (cyclohexane - dichloromethane 3/2). Ή NMR (CDCI3): 6.02 (d, 1H, J= 3.0), 5.86 (dd, 1 H, J= 3.0, 0.9), 6.19 (m, 1H), 5.40 (dd, IH, J= 5.5, 9.3), 4.30 (q, 2H, J = 7.1), 3.58 (dd, IH, J = 9.3, 15.7), 3.45 (dd, IH, J - 5.5, 15.7), 2.23 (s, 3H), 1.30 (t, 3H, J = 7.1). HRMS: calcd for Ci0HnNO5Na: 250.0691; found, 250.0682.
Ethyl 3-(4,5-dimethylfuran-2-yl)-2-nitropropanoate (R-9): As seen by !H NMR, this compound was obtained after heating at 140 °C for 2h in the course of the condensation step and a chromatography over silica gel (cyclohexane - dichloromethane 2/1) as an oil also containing 50 mol% of ethyl-3-(4,5-dimethylfuran-2-yl)acrylate (1.94 g). This was used without further purification in the next step.
Ethyl 3-(5-ethylfuran-2-yl)-2-nitropropanoate (R.-10): Obtained as an oil (3.68 g, 39 % from 5-ethylfurfural) after heating at 140 °C for 2h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 2/1 to 1/6) and (cyclohexane - ethyl acetate 97/3). lH NMR (CDC13): 6.05 (d, I , J = 3.0), 5.88 (m, 1H), 6.19 (m, 1H), 5.42 (dd, 1H, J= 5.7, 9.1), 4.31 (q, 2H, J = 7.0), 3.61 (dd, 1H, 7= 9.1 , 15.7), 3.48 (dd, 1 H, J = 5.7, 15.7), 2.60 (q, 2H, J= 7.0), 1.32 (t, 3H, J= 7.0), 1.21 (t, 3H, J - 7.5). HRMS (m/z): [M- H]" calcd for CnHi4N05: 240.0872; found, 240.0857.
Ethyl 3 -(furan-3 -yl)-2 -ni tropropanoate (R-l 1): Obtained as an oil (0.74 g, 34 % from furan-3 - carbaldehyde) from ethyl (E)-3-(furan-3-yl)-2-nitroacrylate prepared as described before (Org. Biomol. Chem., 2012, 524), after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 9/1). !H NMR (CDC13): 7.40 (m, 1H), 7.34 (m, 1H), 6.30 (m, 1H), 5.26 (dd, 1 H, J = 5.3, 9.4), 4.32 (q, 2H, J - 7.1), 3.39 (dd, 1H, J = 9.4, 15.2), 3.32 (dd, 1H, 7 = 5.4, 15.2), 1.32 (t, 3H, J 7.1 ). HRMS: calcd for C9HnN05Na, 236.0559; found, 236.0567.
Ethyl 2-nitro-3-(thiophen-2-yl)propanoate (R-l 2) Obtained as an oil (3.43 g, 33 % from thiophene-2-carbaldehyde) after heating at 140 °C for 4h in the course of the condensation step and two chromatography over silica gel (cyclohexane - dichloromethane 3/2) and (cyclohexane - ethyl acetate 97/3 to 95/5). Ή NMR (CDC13): 7.23 (dd, 1H, J = 1.3, 5.1), 6.96 (m, 1 H), 6.92 (m, 1 H), 5.35 (dd, 1 H, J= 5.6, 9.2), 4.32 (q, 2H, J = 7.1), 3.83 (dd, 1H, J - 9.2, 15.4), 3.70 (dd, 1H, J = 5.6, 15.4), 1.32 (t, 3H, J = 7.1). HRMS (m/z): [M-H]" calcd for C9H8N04, 228.0331 ; found, 228.0348.
General procedure for the reduction of the a-nitroesters R. The considered a-nitroester (0.023 mol) was dissolved in ethanol (75 mL) and 37 % hydrochloric acid (29 mL, 0.34 mol) and cooled to °C. Powdered zinc (9.05 g, 0.13 mol, less than 10 μι size) was added by portion. This was left to stir overnight, the ethanol was removed under vacuum, the residue was dispersed in water and ethyl acetate, the suspension was made basic with 22% ammonia, this was extracted with ethyl acetate, the organic layer was washed with water, brine, dried over sodium carbonate and concentrated to dryness to yield the amino ester which was in some cases further purified as described below.
Ethyl 2-amino-3-(4-fluorophenyl)propanoate (B-l): Obtained as an oil (1.95 g, 94 %). Ή NMR (CDC13): 7.18 (m, 3H), 7.01 (m, 1 H), 4.17 (q, 2H, 7 = 7.1), 3.68 (dd, I H, 7 = 5.5, 7.6), 3.04 (dd, 1H, J = 5.5, 13.6), 2.86 (dd, 1H, J = 7.6, 13.6), 1.48 (s, 2H), 1.25 (t, 3H, J = 7.1). HRMS: caicd fer CnHi5FN02: 212.1087; found, 212.1069.
Ethyl 2-amino-3-(2-methoxyphenyl)propanoate (B-2): Obtained as an oil (5.16 g, 90 %). Ή
NMR (CDCI3): 7.23 (m, 1H), 7.14 (m, 1H), 6.86-6.92 (m, 2H), 4.19 (q, 2H, J= 7.2), 3.84 (s, 3H), 3.81 (dd, IH, J = 5.8, 8.1), 3.10 (dd, 1H, J= 5.8, 13.3), 2.84 (dd, 1H, J = 8.1 , 13.3), 1.51
(s, 2H), 1.31 (t, 3H, J = 7.1). HRMS: calcd for C12HlgN03: 224.1287; found, 224.1218.
Ethyl 2-am ino-3 -(3 -methox yph enyl)propanoate (B-3): Obtained as an oil (0.81 g, 92 %). ! H NMR (CDCI3): 7.23 (t, 1 H, J - 7.9), 6.77-6.82 (m, 3H), 4.19 (q, 2H, J = 7.1), 3.81 (s, 3H), 3.73 (dd, 1 H, J = 5.2, 7.9), 3.08 (dd, 1 H, J= 5.2, 13.5), 2.84 (dd, IH, J = 7.9, 13.5), 1.51 (s, 2H), 1.31 (t, 3H, J = 7.1). HRMS: calcd for Ci2Hi8N03: 224.1287; found, 224.1260.
Ethyl 2-amino-3-(4-methoxyphenyl)propanoate (B-4): Obtained as an oil (4.2 g, 95 %). Ή NMR (CDCI3): 7.15 (m, 2H), 6.88 (m, 2H), 4.19 (q, 2H, J= 7.1), 3.80 (s, 3H), 3.68 (dd, IH, J = 5.3, 7.7), 3.03 (dd, 1H, J= 5.3, 13.7), 2.83 (dd, 1K, J= 7.7, 13.7), 1.48 (s, 2H), 1.31 (t, 3H, J - 7.1). HRMS: calcd for Ci2Hi8N03: 224.1287; found, 224.1238.
Ethyl 2-amino-3-(4-(benzyloxy)phenyl)propanoate (B-5): Obtained as an oil (2.65 g, 67 %) after a chromatography over silica gel (dichloromethane - ethanol 96/4). Ή NMR (CDCI3): 7.46-7.33 (m, 5H), 7.13 (m, 2H), 6.93 (m, 2H), 5.06 (s, 2H), 4.18 (q, 2H, J - 7.1), 3.68 (dd, IH, J - 5.3, 7.8), 3.04 (dd, IH, J= 5.3, 13.7), 2.84 (dd, HI, J - 7.8, 13.7), 1.47 (s, 2H), 1.27 (t, 3H, J = 7.1 ). HRMS: calcd for C i 8H22N03: 300.1600; found, 300.1591.
Ethyl 2-amino-3-(m-tolyl)propanoate (B-6): Obtained as an oil (0.73 g, 80 %). "Ή NMR (CDCI3): 7.22 (m, IH), 7.02 (m, 3H), 4.19 (q, 2H, J = 7.1), 3.72 (dd, IH, J = 8.0, 5.2), 3.07 (dd, 1H, J= 13.6, 5.2), 2.84 (dd, 1H, J= 13.6, 8.0), 2.34 (s, 3H), 1.50 (s, 2H), 1.27 (t, 3H, J= 7.1). HRMS: calcd for Ci2H,gN02, 208.1338; found, 208.1334.
Ethyl 2-amino-3-(furan-2-yl)propanoate (B-7): Obtained as an oil (4.84 g, 94 %). !H NMR (CDCI3): 7.34 (dd, IH, J = 0.8, 1.9), 6.30 (dd, IH, J = 1.9, 3.2), 6.12 (m, IH), 4.20 (m, 2H), 3.80 (dd, IH, J = 7.3, 5.2), 3.10 (dd, IH, J = 14.9, 5.2), 2.99 (dd, IH, J = 14.9, 7.3), 1.59 (s, 2H), 1.28 (t, 3H, J= 7.2). HRMS: calcd for C9H14N03, 184.0974; found, 184.0955.
Ethyl 2-amino-3-(5-methylfuran-2-yl)propanoate (B-8): Obtained as an oil (1.26 g, 75 %). Ή NMR (CDCI3): 5.97 (d, IH, J = 2.9), 5.85 (dd, IH, J = 2.9, 0.9), 4.24 - 4.14 (m, 2H), 3.72 (dd, IH, J = 7.3, 5.0), 3.03 (dd, IH, J= 14.9, 5.0), 2.91 (dd, IH, J= 14.9, 7.3), 2.24 (s, 3H), 1.57 (s, 2H), 1.27 (t, 3H, J= 7.1). HRMS: calcd for C!0H16NO35 198.1130; found, 198.1089. Ethyl 2-amino-3-(4,5-dimethylfuran-2-yl)propanoate (B-9) Obtained as an oil (0.6 g, 7 % from 4,5-dimethylfurfural). Ή NMR (CDCI3): 5.88 (s, IH), 4.20 (m, 2H), 3.00 (dd, IH, J = 4.8, 14.9), 2.87 (dd, 1H, J= 7.5, 14.9), 2.15 (s, 3H), 1.89 (s, 3H), 1.64 (s, 2H), 1.29 (t, 3H, J =
7.1) . HRMS: calcd for CnH18N03, 212.1287; found, 212.1246.
Ethyl 2-amino-3-(5-ethylfuran-2-yl)propanoate (B-10): Obtained as an oil (1.83 g, 56 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2 to 97/3). Ή NMR (CDC1 ): 6.00 (d, 1H, J= 3.0), 5.87 (d, 1 H, J= 3.0), 4.20 (m, 2H), 3.74 (dd, 1H, J = 7.2, 5.1), 3.05 (dd, 1H, J= 14.9, 5.1), 2.94 (dd, 1 H, J = 14.9, 7.2), 2.24 (q, 2H, J= 7.5), 1.58 (s, 2H), 1.29 (t, 3H, J= 7.2), 1.21 (t, 3H, J= 7.5). HRMS: calcd for C„Hi8N03, 212.1287; found, 212.1261.
Ethyl 2-amino-3 -(furan-3 -yl)propanoate (B-l l): Obtained as an oil (1.4 g, 88 %). Ή NMR (CDC13): 7.38 (m, 1H), 7.31 (m, HI), 6.28 (m, 1 H), 4.20 (q, 2H, J = 7.2), 3.65 (dd, 1H, J = 7.0, 5.3), 2.90 (dd, 1 H, J = 14.3, 5.3), 2.79 (dd, 1H, J = 14.3, 7.0), 1.57 (s, 2H), 1.29 (t, 3H, J = 7.2). HRMS: calcd for C9H14N03, 184.0974; found, 184.0951.
Ethyl 2-amino-3-(thiophen-2-yl)propanoate (B-12): Obtained as an oil (2.5 g, 58 %) after dilution of the oil in ethyl acetate, extraction with IN hydrochloric acid and, upon basification of this aqueous phase with 22 % ammonia an extraction with ethyl acetate. The organic layer was then washed with brine, dried over sodium carbonate and concentrated to dryness. Ή NMR (CDCI3): 7.19 (dd, I II, J - 1.1 , 5.1 ), 6.96 (dd, 1H, J = 3.3, 5.1), 6.88 (m, IH), 4.21 (q, 2H, J = 7.1), 3.72 (dd, 1 H, J - 7.2, 4.8), 3.30 (dd, 1H, J = 14.7, 4.8), 3.17 (dd, 1H, J = 14.7,
7.2) , 1.57 (s, 2H), 1.29 (t, 3H, J - 7.1). HRMS: calcd for C9H,4N02S, 200.0745; found, 200.0726.
General procedures for the synthesis of a-aminoesters B via a-oxime esters S, preparation of a-oxime esters S. Step 1 , Knoevenagel condensation of diethyl malonate and aldehyde, preparation of substituted diethyl 2-methylenemalonate. Under an inert atmosphere, the considered aldehyde (0.048 mol), diethyl malonate (7.54 g, 0.048 mol), acetic acid (0.14 g, 0.002 mol), piperidine (0.2 g, 0.002 mol) and 4A molecular sieve (15 g) were heated in dry ethanol (20 mL, dried over 4 Angstrom molecular sieves) without stirring at 60 °C for 10 hours and Ή NMR spectra of a crude sample usually pointed out the complete disappearance of the starting aldehyde. Step 2, reduction of the substituted diethyl 2-methylenemalonate, preparation of the substituted malonate. The suspension containing the 4A molecular sieve described above was filtered, the 4 A molecular sieve washed with dry ethanol. To the resulting ethanol solution (160 mL total volume) 10 % palladium over charcoal (1.27 g, 0.001 mol) was added and this was stirred overnight under a hydrogen atmosphere (1 atm). The resulting suspension was filtered, the filtrate concentrated to dryness to give fairly pure substituted malonate. Alternatively, as specified in some of the following examples, this hydrogenation was achieved using sodium boron hydride (1.1 eq.). Two procedures were devised, the first one proceeding by the addition of sodium borohydride at 0°C in small portions to an ethanolic solution and stirring at 0°C for two hours before working up, the second one by adding sodium boron hydride at 4 °C and stirring overnight at this temperature before using the following work up procedure This was followed by its acidification with acetic acid (1.2 equivalent), dilution in an excess of water and extraction with ethyl acetate. The organic layer was then washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield the crude substituted malonate. Step 3, preparation of a- oxime esters from the substituted malonates. The crude substituted malonate (0.0422 mol) was dissolved in ethanol (60 mL, dried over 4 A molecular sieve) and cooled to 0°C. A 21 % solution of sodium ethoxide in ethanol was added (18.4 mL, 0.0493 mol) followed by a slow addition of isoamyl nitrite (6.2 mL, 0.046 mol). This was stirred at 0°C for two hours, made slightly acid by the addition of IN hydrochloric acid and diluted in water (200 mL). This solution was extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield the crude a-oxime esters further purified as described below.
Ethyl 3-cyclopentyl-2-(hydroxyimino)propanoate (S-l): Obtained as a white solid (2.50 g, 35 % from cyclopentanecarbaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 85/15). 'Ή NMR (CDC ): 9.51 (s, 1H), 4.32 (q, 2H, J = 7.0), 2.67 (d, 2H, J = 7.6), 2.20 (m, 1H), 1.70 (m, 4H), 1.53 (m, 2H), 1.36 (t, 3H, J = 7.0), 1.25 (m, 2H). HRMS: calcd for C!0Hi7NO3Na: 222.1 106; found, 222.1 164.
Ethyl 3 -cyclohexyl -2-(hydroxyim ino)propanoate (S-2): Obtained as a white solid (4.34 g, 50 % from cyclohexanecarbaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1). Ή NMR (CDC13): 9.85 (s, 1H), 4.31 (q, 2H, J= 7.2), 2.55 (d, 2H, J= 7.1), 1.70 (m, 6H), 1.36 (t, 3H, J = 7.0), 1.20 (m, 3H), 1.04 (m, 2H). HRMS: calcd for CnH19N03Na: 236.1263; found, 236.1291.
Ethyl 3-(bicyclo[2.2.1]heptan-2-yl)-2-(hydroxyimino)propanoate (S-3): Obtained as an oily mixture of diastereoisomers (1.39 g, 25 % from the two isomers of norbornane-2- carbox aldehyde) after two chromatography over silica gel (cyclohexane - ethyl acetate 7/1 to 4/1) and (dichloromethane - ethanol 99/1). ]H NMR (CDC13): 4,33 (m, 4H, arbitrary value), 2.66 (m, 3.2H), 2.50 (m, 0.9H), 2.22 (m, 3.3H), 2.08 (m, 1.2H), 1.96 (m, 0.7H), 1.85 (m, 0.8H), 1.73 (m, 2.8H), 1.48 (m, 4.5H), 1.34 (m, 10.8H), 1.15 (m, 4.5H), 0.79 (m, 1.3H). . HRMS: calcd for Ci2Hi9N03Na, 248.1263; found, 248.1265.
Ethyl 2-(hydroxyimino)-3-(tetrahydrofuran-2-yl)propanoate (S-4): This compound was obtained when using 10 % Pd/C for the hydrogenation step and letting it run 48 hours. Out a 12.8 g batch, only a sample was purified by a chromatography (dichloromethane - ethanol 97/3) to give a white solid. Ή NMR (CDC13): 9.74 (s, 1 H), 4.32 (q, 2H, J = 7.2), 4.28 (m, 1H), 3.90 (m, 1H), 3.74 (m, 1H), 2.96 (dd, 1H, J = 5.5, 12.8), 2.80 (dd, 1H, J = 5.9, 12.8), 1.88 (m, 3H), 1.62 (m, 1H), 1.35 (t, 311, J = 7.2). HRMS: calcd for C9Hi5N04Na: 224.0899; found, 224.0874.
Ethyl 2-(hydroxyimino)-3-(3-methylthiophen-2-yl)propanoate (S-5): Obtained as a yellow solid (1.38 g, 32 % from 3-methylthiophene-2~carbaldehyde), using sodium boron hydride at 0°C, after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). Ή NMR (CDC13): 9.88 (s, 1H), 7.06 (d, 1 H, J 5.1 ), 6.77 (d, 1H, J = 5.1), 4.33 (q, 2H, J = 7.2), 4.09 (s, 211), 2.28 (s, 3H), 1.36 (t, 3H, J = 7.2). HRMS: calcd for Ci0H!3NO3SNa: 250.0514; found,
250.0513.
Ethyl 3-(5-ethylthiophen-2-yl)-2-(hydroxyimino)propanoate (S-6): Obtained as a oil (1.36 g, 29 % from 5-ethylthiophene-2-carbaldehyde), using sodium boron hydride at 0°C, after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). !H NMR (CDCI3): 10.06 (s, 1H), 6.75 (m, 1H), 6.59 (m, 1H), 4.34 (q, 2H, J= 7.2), 4.09 (s, 2H), 2.78 (m, 2H), 1.38 (t, 3H, J= 7.2), 1.28 (t, 3H, J = 7.5). HRMS: calcd for CnHi5N03SNa: 264.0670; found, 264.0676. Ethyl 3-(4,5-dimethylthiophen-2-yl)-2-(hydroxyimino)propanoate (S-7): Obtained as an oil (0.69 g, 31 % from 4,5-dimethylthiophene-2-carbaldehyde), using sodium boron hydride at 0°C, after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). Ή NMR (CDCI3): 9.77 (s, 1H), 6.62 (s, 1 H), 4.34 (q, 2H, J = 7.2), 4.03 (s, 2H), 2.28 (s, 3H), 2.06 (s, 3H), 1.37 (t, 3H, J= 7.2). HRMS: calcd for CnHi5NS03Na: 264.0670; found, 264.0656. Ethyl 2-(hydroxyimino)-3-(5-(trifluoromethyl)furan-2-yl)propanoate (S-8): Obtained as a white solid (0.62 g, 36 % from 5 - tri l uoromethyl furan-2-carbaldehyde) , using sodium boron hydride at 0°C, after two chromatographies over silica gel (dichloromethane - ethanol 99/1) and (cyclohexane - ethyl acetate 5/1). Ή NMR (CDC13): 9.70 (s, 1 H), 6.72 - 6.64 (m, 1H), 6.21 - 6.15 (m, 1H), 4.33 (q, 2H, J= 7.1), 4,05 (s, 2H), 1.34 (t, 3H, J= 7.1). HRMS: calcd for C10H11F3NO4: 266.0640; found, 266.0584.
Ethyl 2-(hydroxyimino)-3-(pyridin-2-yl)propanoate (S-9): Obtained as a solid (4.94 g, 49 % from picolinaldehyde) after a chromatography (dichloromethane - ethanol 97/3). Ή NMR (CDCI3): 10.53 (s, 1 H), 8.53 (m, 1H), 7.61 (dt, 1H, J= 1.8, 7,5), 7.29 (m, 1H), 7,17 (m, 1H), 4.29 (q, 2H, J = 7.1), 4.23 (s, 211), 1.30 (t, 3H, J = 7.1). HRMS: calcd for Ci0H!3N2O3: 209.0926; found, 209.0857.
Ethyl 2-(hydroxyimino)-3-(pyridin-3-yl)propanoate (S-10): Obtained as a tan solid (7.19 g, 43 % from nicotinaldehyde) after a chromatography (dichloromethane - ethanol 97/3 to 96/4). 'H NMR (CDCI3): 12,79 (s, 1H), 8.65 (m, 1H), 8.46 (dd, 1 H, J = 1.6, 4.9), 7.74 (m, 1H), 7.22
(m, 1H), 4.30 (q, 2H, J = 7.1), 4.01 (s, 2H), 1.32 (t, 3H, J = 7.1). HRMS: calcd for CioHi3N203: 209.0926; found, 209.0921.
Ethyl 2-(hydroxyimino)-3-(2-(trifluoromethyl)phenyl)propanoate (S-11): Obtained as a white solid (1.05 g, 33 % from 2-(trifluoromethyl)benzaldehyde), 90 % pure) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1 to 3/1). Ή NMR (CDCI3): 9.66 (s, 1H), 7.68 (d(br), 1H, J = 7.5), 7.45 (m, 1H), 7.34 (m, 1H), 7.16 (d(br), 1H, J = 7.7), 4.29 (q, 2H, J = 7.2), 4.23 (s, 2H), 1.27 (t, 3H, J = 7.2). HRMS: calcd for Ci2Hi2F3N03Na: 298.0667; found, 298.0660.
Ethyl 2-(hydroxyimino)-3-(3-(trifluoromethyl)phenyl)propanoate (S-12): Obtained as a white solid (1.3 g, 41 % from 3-(trifluoromethyl)benzaldehyde)) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1 to 3/1). Ή NMR (CDC13): 9.87 (s, 1H), 7.62 (s(br), 1H), 7.50 (m, 2H), 7.42 (m, 1H), 4.32 (q, 2H, J = 7.0), 4.05 (s, 2H), 1.35 (t, 3H, J = 7.0). HRMS: calcd for Cl2Hi2F3N03Na: 298.0667; found, 298.0643.
Ethyl 2-(hydroxyimino)-3-(4-(trifluoromethyl)phenyl)propanoate (S-13): Obtained as a white solid (4.61 g, 62 % from 4-(trifluoromethyl)benzaldehyde)) after a chromatography over silica gel (dichloromethane - ethaeol 99/1). lH NMR (CDCI3): 9.76 (s, 1H), 7.55 (m, 2H), 7.45 (m, 2H), 4.32 (q, 2H, J = 7.1), 4.05 (s, 2H), 1.35 (t, 3H, J = 7.1). HRMS: calcd for Ci2H13F3N03: 276.0847; found, 276.0834.
Ethyl-3-(4-chlorophenyl)-2-(hydroxyimino)propanoate (S-14): Obtained as a white solid (2.31 g, 37 % from 4-chlorobenzaldehyde), using sodium boron hydride at 4°C overnight, after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1). Ή NMR (CDC13): 9.70 (s, 1H), 7.27 (m, 4H), 4.31 (q, 2H, J = 7.1), 3.96 (s, 2H), 1.35 (t, 3H, J = 7.1). HRMS: calcd for d 1H14ClN02Na, 264.0403; found, 264.0437.
Ethyl 3-(2-chlorophenyl)-2-(hydroxyimino)propanoate (S-15): Out of a 13.1 g batch, using sodium boron hydride at 4°C, only a 0.5 g sample was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 4/1) and a recrystallization in cyclohexane to give a white solid (0.36 g). Ή NMR (CDCI3): 9.47 (s, IH), 7.38 (m, I H), 7.18 (m, 3H), 4.30 (q, J = 7.1 Hz, 2H), 4.13 (s, 2H), 1.31 (t, J = 7.1 Hz, 3H). HRMS: calcd for CnH12ClN03Na: 264.0403; found, 264.0401.
Ethyl-3-(4-bromophenyl)-2-(hydroxyimino)propanoate (S-16): Obtained as a white solid (1.43 g, 42 % from 4-bromobenzaldehyde), using sodium boron hydride at 4°C overnight, after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1). Ή NMR (CDC13): 9.70 (s, IH), 7.42 (m, 2H), 7.22 (m, 2H), 4.31 (q, 211, J = 7.1), 3.96 (s, 2H), 1.32 (t, 3H, J =
7.1). HRM.S: calcd for CnH13BrN03, 286.0079; found, 286.0056.
Ethyl 3-(2-fiuorophenyl)-2-(hydroxyimino)propanoate (S-17): Obtained as a white solid (3.11 g, 57 % from 2-fluorobenzaldehyde), after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1). Ή NMR (CDC13): 10.20 (s, IH), 7.22 (m, 2H), 7.05 (m, 2H), 4.30 (q, 2H, J = 7.1), 4.04 (s, 2H), 1.31 (t, 3H, J= 7.1). HRMS: calcd for C„Hi2FN03Na: 248.0699; found, 248.0704.
Ethyl 3-(3-fluorophenyl)-2-(hydroxyimino)propanoate (S-18): Obtained as a white solid (9.46 g, 65 % from 3-fluorobenzaldehyde) after a chromatography (cyclohexane - ethyl acetate 5/1). Ή NMR (CDCI3): 10.02 (s, IH), 7.25 (m, IH), 7.10 (m, IH), 7.06 (m, IH), 6.92 (m, IH), 4.32 (q, 2H, J = 7.2), 3.99 (s, 211), 3.74 (m, IH), 1.35 (t, 3H, J = 7.2). HRMS: calcd for Ci ,Hi2FN03Na: 248.0699; found, 248.0699.
Ethyl 3-(2,4-difluorophenyl)-2-(hydroxyimino)propanoate (S-19): Obtained as a white solid
(2.21 g, 50 % from 2,4-difluorobenzaldehyde) after a chromatography (cyclohexane - ethyl acetate 6/1). !H NMR (CDC13): Ή NMR (CDCI3): 9.72 (s, IH), 7.22 (m, IH), 6.80 (m, 2H), 4.30 (q, 211, J = 7.1), 3.98 (s, 2H), 1.33 (t, 3H, J = 7.1). HRMS: calcd for CnHi iF2N03Na:
266.0605; found, 266.0601.
Ethyl 2-(hydroxyimino)-3-(o-tolyl)propanoate (S-20): Obtained as a white solid (3.38 g, 61 % from 2-methylbenzaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1). Ή NMR (CDCI3): 10.09 (s, IH), 7.16 (m, 4H), 4.28 (q, 2H, J = 7.1), 4.00 (s, 2H), 2.41 (s, 3H), 1.31 (t, 3H, J = 7.1). HRMS: calcd for C,2Hi5N03Na: 244.0950; found, 244.0942.
Ethyl 2-(hydroxyimino)-3-(p-tolyl)propanoate (S-21): Obtained as a white solid (3.31 g, 60 % from 4-methylbenzaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1). !H NMR (CDC13): 10.07 (s, IH), 7.24 (m, 2H), 7.11 (m, 2H), 4.31 (q, 2H, J = 7.1), 3.97 (s, 2H), 2.35 (s, 3H), 1.35 (t, 3H, J = 7.1). HRMS: calcd for CI2H15N03Na:
244.0950; found, 244.0944.
Ethyl 2-(hydroxyimino)-3-(4-isopropylphenyl)propanoate (S-22): Obtained as a solid (2.04 g,
48 % from 4-isopropylbenzaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). 1H NMR (CDCI3): 9.74 (s, IH), 7.26 - 7.22 (m, 2H), 7.16 - 7.11 (m, 2H), 4.29 (q, 2H, J= 7.1), 3.95 (s, 2H), 2.87 (hept, 1H, J= 6.9), 1.32 (t, 3H, J= 7.1), 1.23 (d, 6H, J = 6.9). HRMS: calcd for C14Hi9N03Na, 272.1263; found, 272.1258.
Additional synthesis a-oxime esters S, via carbon-carbon coupling reactions Preparation ethyl 3-(5-cyc]opropylfiiran-2-yl)-2-(hydroxyimino)propanoate (S-23). Step 1 , synthesis of diethyl 2-((5-bromoraran-2-yl)methylene)malonate. Diethyl 2-(furan-2- ylmethylene)malonate (Coll. Czech. Chem. Commun., 1987, 2534) (2 g, 8.39 mmol) was dispersed in acetic acid (20 mL) and N-bromosuccinimide (2,98 g, 16.78 mmol) was then added. This was stirred at room temperature overnight, diluted in ethyl acetate, washed extensively with water, brine, dried over magnesium sulfate and concentrated to dryness. The residue was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 9/1) to give the brominated derivative as an orange solid (1.1 g, 41 %). !H NMR (CDC13): Ή NMR (CDCI3): 7.35 (s, 1 H), 6.71 (d, 1 H, J = 3.5), 6.44 (d, 1H, J = 3.5), 4.41 (q, 2H, J = 7.1), 4.29 (q, 2H, J = 7.1), 1 .41 (t, 3H, J = 7.1 ), 1.33 (t, 3H, J = 7.1). HRMS: calcd for€12ΗΙ3ΒΓ05Ν3, 338.9844; found, 338.9868. Alternatively, a Knoevenagel condensation between 5- bromofurfural and diethylmalonate using the conditions described above also gave this compound (4.72 g, 87 %). Step 2: synthesis of diethyl 2-((5-cyclopropylfuran-2- yl)methylene)malonate: Diethyl 2-((5-bromofuran-2-yl)methylene)malonate (4.7 g, 14.8 mmol), cyclopropylboronic acid (1.66 g, 19.3 mol) and cesium carbonate (18.84 g, 57.8 mmol) were dispersed in a 95/5 mixture of toluene and water ( 120 mL). This was degassed by blowing a gentle stream of argon into the suspension, [ 1 , 1 '-bis(diphenylphosphino)ferrocene] dichloropalladium complexed with dichloromethane (0.30 g, 0.36 mmol) was added and this was heated to reflux under argon for 50 minutes. The resulting dark solution was diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The residue was purified by a chromatography over silica gel (cyclohexane ·■■- ethyl acetate 8/1) to give the cyclopropyl-bearing derivative as an oil (3.12 g, 75 %). Ή NMR (CDCI3): 7.32 (s, 1H), 6.65 (d, 1H, J = 3.4), 6.09 (d, 1H, J = 3.4), 4.37 (q, 2H, J = 7.1 ), 4.25 (q, 2H, J - 7.1 ), 1 .89 (tt, 1H, J = 8.4, 5.0), 1.37 (t, 3H, J = 7.1 ), 1.30 (t, 3H, J = 7.1), 1.01 - 0.93 (m, 2H), 0.88 - 0.79 (m, 2H). HRMS: calcd for C15Hi805Na, 301.1052; found, 301.1034. Step 3 and 4: synthesis of ethyl 3-(5-cyclopropylfuran-2-yl)-2-(hydroxyimino)propanoate (S- 23): from the precedent compound, by using the general preparation of oxime esters described above, using sodium boronhydride overnight at 4°C for the reduction step, this compound was obtained as an orange solid (1.09 g, 44 % from diethyl 2-((5-cyclopropylfuran-2- yl)methylene)malonate) after a chromatography over silica gel (cyclohexane - ethyl acetate 4/1 ). Ή NMR (CDCI3): 1H NMR (CDCI3): 9.42 (bs, IH), 5.97 (m, IH), 5.84 (d, 1 H, J= 8.1), 4.33 (q, 2H, J = 7.1), 3.97 (s, 2H), 1.84 (m, IH), 1.36 (t, 3H, J = 7.1 ), 0.84 (m, 211), 0.73 (m, 2H). HRMS (m/z): HRMS: calcd for daH^NC^Na, 260.0899; found, 260.0892. Preparation of ethyl-2-(hydroxyimino)-3-(3-propylphenyl)propanoate (S-24): Step 1, preparation of diethyl 2-(4-bromobenzylidene)malonate. By using a noevenagel condensation between 3-bromobenzaldehyde and diethylmalonate using the conditions described above this compound was obtained as an oil which was directly used in the next step. Step 2, preparation of diethyl 2-(3-cyclopropylbenzylidene)malonate. By using the protocol described above for the preparation of diethyl 2-((5-cyclopropylfuran-2- yl)methylene)malonate, this compound was obtained as an oil (4.68 g, 60 % from 3- bromobenzaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). 'H NMR (CDCI3): 7.72 (s, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.1 1 (m, 1H), 4.36 (q, 2H, J - 7.1), 4.32 (q, 2H, J= 7.1), 1.90 (m, IH), 1.35 (t, 3H, J = 7.1), 1.32 (t, 3H, J= 7.1). Step 3, preparation of diethyl 2-(3-propylbenzyl)malonate. A two days-long catalytic hydrogenation with 10 % palladium over charcoal as described above, gave this compound which was directly used in the final step. Step 4, an oximation reaction of the crude diethyl 2-(3- propylbenzyl)malonate using the general condition described above gave S-24 (0.2 g, 14 % from diethyl 2-((5-cyclopropylfuran-2-yl)methylene)malonate) as a white solid after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). Ή NMR (CDCI3): 9.74 (bs, 111), 7.19 (m, 3H), 7.05 (m, IH), 4.31 (q, 2H, J - 7.2), 3.99 (s, 2H), 2.58 (m, 2H), 1.65 (m, 2H), 1.34 (t, 311, J - 7.2), 0.96 (t, 3H, J = 7.3). HRMS: calcd for Ci4Hi9N03Na, 272.1263; found, 272.1250.
Preparation of ethyl-2-(hydroxyimino)-3-(4-propylphenyl)propanoate (S-25): By using the same procedures described for the preparation of ethyl-2-(hydroxyimino)-3-(3- propylphenyl)propanoate (S-24), this isomer was obtained (2.64 g, 56 % from 4- bromobenzaldehyde) as a white solid after a chromatography over silica gel (cyclohexane - ethyl acetate 6/1). Ή NMR (CDC13): 9.64 (s, IH), 7.26 (m, 2H), 7.1 1 (m, 2H), 4.31 (q, 211, J « 7.2), 3.98 (s, 2H), 2.57 (m, 2H), 1.64 (m, 2H), 1.34 (t, 311, J - 7.2), 0.95 (t, 3H, J -7.3). HRMS: calcd for CI4Hi9N03Na, 272.1263; found, 272.1276.
Ethyl 3-(3-cyclopropylphenyl)-2-(hydroxyimino)propanoate (S-26). By using the general preparation of oxime esters described above, using sodium borohydride overnight at 4°C for the reduction step, this compound was obtained as a colourless oil (1.72 g, 50 % from diethyl 2-(3-cyclopropylbenzylidene)malonate) after a chromatography over silica gel (cyclohexane - ethyl acetate 6/1). Ή NMR (CDCI3): 9.99 (bs, IH), 7.20 (m, 3H), 6.91 (m, III), 4.31 (q, J = 7.2 Hz, 2H), 3.97 (s, 2H), 2.58 (m, 2H), 1.88 (m, IH), 1.34 (t, 3H, J= 7.2), 0.96 (m, 2H), 0.69 (m, 2H). HRMS: calcd for C14Hi7N03Na, 270.1 106; found, 270.1 112. Ethyl 3-(4-cyclopropylphenyl)-2-(hydroxyimino)propanoate (S-27). By using the general preparation of oxime esters described above, using sodium borohydride overnight at 4°C for the reduction step, this compound was obtained as a pale yellow solid (1.03 g, 27 % from 4- bromobenzaldehyde) after a chromatography over silica gel (cyclohexane - ethyl acetate 6/1). !H MR (CDCI3): 9.31 (s, IH), 7.22 (m, 2H), 6.99 (m, 2H), 4.29 (q, J = 7.2 Hz, 211), 3.95 (s, 2H), 1.86 (m, I H), 1.33 (t, J = 7.2 Hz, 3H), 0.93 (m, 2H), 0.67 (m, 2H). HRMS: calcd for Ci4H17N03Na, 270.1106; found, 270.1109.
Additional synthesis a-oxime esters using other methods
Figure imgf000060_0001
Ethyl 3-(4,5-dimethyloxazol-2-yl)-2-(hydroxyimino)propanoate (S-28): Under an inert atmosphere 2,4,5-trimethyloxazole (1.05 g, 9.44 mmol) was dissolved in dry tetrahydrofuran
(10 rriL). The solution was cooled to -78 °C with a dry ice bath and 2N lithium diisopropylamine in tetrahydrofuran (4.7 mL, 9.44 mmol) was added. This was stirred 5 minutes and diethyloxalate (1.28 mL , 9.92 mmol) was added and the solution was allowed to warm back to 20 °C and further stirred for 10 minutes. The solution was diluted in water, extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The resulting oil was dissolved in ethanol (100 mL), hydroxylamine hydrochloride (2.76 g, 39.7 mmol) and dry pyridine (3.3 mL, 40.8 mmol) were added and this was heated to reflux for 6 hours. The resulting suspension was diluted in water, extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The residue was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 1/1) and the corresponding fraction was dispersed in boiling cyclohexane and filtered after cooling to yield the target oxime ester as a white powder (0.15 g, 7 %). !H NMR (CDCI3): 11.99 (s, IH), 4.30 (q, 2H, J = 7.2), 4.08 (s, 2H), 2.20 (d, 3H, J = 0.8), 2.05 (d, 3H, J = 0.8), 1.31 (t, 3FL J = 7.2). Long distance correlation experiments established the regioselectivity of the reaction. HRMS: calcd for C10H14N7O4, 227.1032; found, 227.1035.
Ethyl 2-(hydroxyimino)-3-phenylbutanoate (S-29): Step 1 : preparation of diethyl 2-(l- phenyIethyl)malonate: under a calcium chloride guard diethylmalonate (5.32 g, 0.033 mol) was dissolved in dry DMF (50 mL, dried over 4A molecular sieves) and 60 % sodium hydride in mineral oil (1.39 g, 0.0348 mol) was added portion-wise while maintaining the solution temperature at 20 °C with a water bath. This was stirred until the end of hydrogen evolution and (l-chloroethyl)benzene 4.8 mL, 0.036 mol) was added. This was stirred for 7 days, diluted in water and ethyl acetate, the organic layer was washed 5 times with water, brine, dried over magnesium sulfate and concentrated to dryness under high vacuum to remove unreacted diethylmalonate and ( 1 -chloroethyl)benzene to give an oil (6.22 g) pure enough for the next step. Step 2: reaction with isoamylnitrite. Under an inert atmosphere, 4.68 g of the resulting oil was dissolved in dry ethanol (50 mL, dried over 4 A molecular sieves) and cooled at 0 °C with ice. A solution of 21 % sodium ethanolate in ethanol (7.7 mL, 0.0207 mol) was added followed by the isoamylnitrite (2.6 mL, 0.0194 mol). This was allowed to warm to room temperature and stirred for 16 hours. The resulting solution was then treated as described above for the general preparation of a-oxime esters and obtained as an oil (1.07 g, 14 % from diethylmalonate) after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). Ή NMR (CDCI3, for once it is a separable but slowly equilibrating mixture of the two oxime isomers): 10.69 (s, 0.2H), 9.72 (s, 0.8H), 4.82 (q, 0.8H, J = 7.2), 4.21 (m, 2H), 4.02 (q, 0.2H, J= 7.2), 1.68 (d, 2.411, J 7.2), 1.54 (d, 0.6II, J= 7.2), 1.26 (t, 2.4H, J= 7.3), 1.15 (t, 0.6H, J= 7.3). HRMS: calcd for Ci2Hi5N03Na, 244.0950; found, 244.0939.
General method for the reduction of the α-oxime esters S into o-aminoesters B. The considered substituted a-oxime ester (0.065 mol) was dissolved in ethanol (70 mL) and 37 % hydrochloric acid (49 mL, 0.58 mol) and the solution was cooled to °C. Powdered zinc (12.9 g, 0.19 mol, less than 10 μιη size) was added by portion. This was left to stir for 2 hours, diluted in water, made basic with 22% ammonia, extracted with ethyl acetate, washed with water, brine and concentrated to dryness to yield the pure aminoester. If a crude oxime was used, the organic layer was counter-extracted with IN hydrochloric acid, the acidic water phase made basic again with 22% ammonia and extracted with ethyl acetate, this organic layer washed with water, brine, dried over sodium carbonate and concentrated to dryness to yield the amino ester which was in some cases further purified as described below.
Ethyl 2-amino-3-cyclopentylpropanoate (B-13): Obtained as an oil (1.16 g, 85 %). Ή NMR (CDCI3): 4.17 (q, 2H, J = 7.2), 3.42 (dd, 1H, J = 5.8, 8.1), 1.93 (m, 1H), 1.80 (m, 2H), 1.75- 1.48 (m, 8H), 1.27 (t, 3H, J = 7.2), 1.12 (m, 2H). HRMS: calcd for CIOH2oN02: 186.1494; found, 186.1491.
Ethyl 2-amino-3-cyclohexylpropanoate (B-14): Obtained as an oil (2.35 g, 92 %). Ή NMR (CDCI3): 4.17 (m, 2H), 3.48 (dd, 1H, J = 5.8, 8.7), 1.72 (m, 511), 1.61-137 (m, 5H), 1.28 (t, 3H, J= 7.3), 1.28-1.10 (m, 3H), 0.92 (m, 211). HRMS: calcd for CnH22N02: 200.1651 ; found, 200.1648. Ethyl 2-arnino-3-(bicyclo[2.2. l]heptan-2-yl)propanoate (B-15): Obtained as an oil (1.21 g, 92
%) as a mixture of four diastereoisomers (as seen by four !3C COOEt signals). 1 H NMR (CDCI3): 4.17 (m, 2H), 3.37 (m, 1H), 2.16 (m, 1.6H), 1.91 (m, 1H), 1.75 (m, 1.6H), 1.50 (m, 5H), 1.28 (m, 5.9H), 1.10 (m, 2.4H), 0.64 (m, 0.6H). HRMS: calcd for Ci2H22 02: 212.1651; found, 212.1650.
Ethyl 2-amino-3-(tetrahydrofuran-2-yl)propanoate (B-16): Obtained as an oil (5.68 g, 36 % from furfural) as a mixture of two diastereoisomers in a 3/2 ratio. Ή NMR (CDCI3): Major isomer: 4.17 (q, 2H, J= 7.3), 4.01 (m, 1H), 3.85 (m, 1 H), 3.72 (m, I H), 3.62 (dd, I H, J= 8.9, 3.5), 2.03 (m, 1 H), 1.89 (m, 2H), 1.68 (ddd, 2U, J= 3.5, 8.9, 13.8), 1.68 (s, 2H), 1.51 (m, 1H), 1.27 (t, 3H, J = 7.3). Minor isomer: 4.17 (q, 2H, J = 7.2), 4.01 (m, 1H), 3.85 (m, 1 H), 3.72 (m, 1H), 3.56 (dd, 1 H, J = 7.3, 5.4), 2.03 (m, IH), 1.89 (m, 2H), 1.80 (m, 2H), 1.61 (s, 2H), 1.49 (m, I H), 1.27 (t, 3H, J = 7.2). HRMS: calcd for C9H18N03: 188.1287; found, 188.1258. Ethyl 2-amino-3-(4,5-dimethylthiophen-2-yl)propanoate (B-17): Obtained as an oil (0.46 g, 87 %). Ή NMR (CDCI3): 6.54 (s, IH), 4.22 (d, 2H, J= 7.1), 3.66 (dd, IH, J= 4.8, 7.4), 3.17 (dd, IH, J = 4.8, 14.5), 3.03 (dd, 1 H, J = 7.4, 14.5), 2.29 (s, 3H), 2.07 (s, 3H), 1.59 (s, 2H), 1.30 (t, 3H, J = 7.1). HRMS: calcd for CnH!8N02S: 228.1058; found, 228.1053.
Ethyl 2-amino-3-(3-methylthiophen-2-yl)propanoate (B-18): Obtained as an oil (1.10 g, 89 %). Ή NMR (CDCI3): 7.08 (d, IH, J= 5.1), 6.80 (d, I H, J= 5.1), 4.20 (m, 2H), 3.70 (dd, IH, J = 5.1, 7.6), 3.23 (dd, IH, J = 5.1 , 14.7), 3.04 (dd, IH, J = 7.6, 14.7), 2.20 (s, 3H), 1.59 (s, 2H), 1.28 (t, 3H, J = 7.6). HRMS: calcd for Ci0H!6NO2S: 214.0902; found, 214.0901.
Ethyl 2-amino-3-(5-ethylthiophen-2-yl)propanoate (B-19): Obtained as an oil (1.18 g, 92 %). Ή NMR (CDCI3): 6.66 (m, IH), 6.61 (m, IH), 4.21 (q, 2H, J = 5.1 , 7.2), 3.68 (dd, IH, J = 4.8, 7.3), 3.21 (dd, IH, J= 4.8, 14.7), 3.04 (dd, IH, J = 7.3, 14.7), 2.80 (m, 2H), 1.62 (s, 2H), 1.30 (t, 3H, J = 7.1), 1.29 (t, 3H, J = 7.5). HRMS: calcd for CnHl8N02S: 228.1058; found, 228.1055.
Ethyl 2-amino-3-(o-tolyl)propanoate (B-20): Obtained as an oil (2.69 g, 85 %). Ή NMR (CDCI3): 7.16 (m, 4H), 4.17 (q, 2H, J = 7.2), 3.71 (dd, IH, J = 8.7, 5.6), 3.14 (dd, IH, J = 13.8, 5.6), 2.84 (dd, IH, J = 13.8, 8.7), 2.82 (s, 3H), 1.24 (t, 3H, J = 7.2). HRMS: calcd for C]2Hi8N02: 208.1338; found, 208.1332.
Ethyl 2-amino-3-(p-tolyl)propanoate (B-21): Obtained as an oil (2.70 g, 87 %). Ή NMR (CDCI3): 7.1 1 (m, 4H), 4.19 (q, 2H, J = 7.1), 3.70 (dd, IH, J = 7.9, 5.3), 3.07 (dd, IH, J = 13.6, 5.3), 2.84 (dd, IH, J = 13.6, 7.9), 2.34 (s, 311), 1.28 (t, 3H, J = 7.1 ). HRMS: calcd for Ci2i½N02: 208.1338; found, 208.1333. Ethyl 2-amino-3-(pyridin-2-yl)propanoate (B-22): Obtained as an oil (2.21 g, 56 %). !H NMR (CDCI3): 8.55 (m, 1H), 7.61 (dt, 1H, J = 1.9, 7.6), 7.18 (m, 1H), 7.15 (m, 1 H), 4.18 (q, 2H, J = 7.2), 3.98 (dd, I H, J= 4.8, 8.2), 3.28 (dd, 1H, J = 4.8, 14.2), 3.04 (dd, 1H, J = 8.2, 14.2), 1.70 (s, 2H), 1.24 (t, 3H, J = 7.2). HRMS: calcd for€ιοΗ15Ν202: 195.1 134; found, 195.1062. Ethyl 2-amino-3-(pyridin-3-yl)propanoate (B-23): Obtained as an oil (4.07 g, 63 %). Ή NMR (CDCI3): 8.49 (m, 2H), 7.56 (m, 1 H), 7.22 (ddd, 1H, J = 0.8, 4.8, 5.6), 4.16 (q, 2H, J = 7.2), 3.70 (dd, IH, J = 5.5, 7.5), 3.05 (dd, 1 H, J = 5.5, 13.5), 2.88 (dd, IH, J = 7.5, 13.5), 1.46 (s, 2H), 1.23 (t, 3H, J = 7.2). HRMS: calcd for C10H15N2Q.: 195.1 134; found, 195.1 131.
Ethyl 2-amino-3-(2-(trifluoromethyl)phenyl)propanoate (B-24): Obtained as an oil (0.72 g, 78 %, 90 % pure). !H NMR (CDC13): 7.58 (d, 1 H, J = 8.1), 7.41 (m, 1H), 7.32 (d, IH, J = 7.7), 7.26 (m, IH), 4.07 (m, 2H), 3.66 (dd, IH, J= 5.7, 8.9), 3.21 (dd, IH, J= 5.7, 14.3), 2.86 (dd, IH, J = 8.9, 14.3), 1.45 (s, 2H), 1.13 (t, 3H, J = 7.1). HRMS: calcd for C12H15F3NO2: 262.1055; found, 262.1082.
Ethyl 2-amino-3-(3-(trifluoromethyl)phenyl)propanoate (B-25): Obtained as an oil (0.75 g, 78 %). Ή NMR (CDCI3): 7.51 (m, IH), 7.48 (m, I H), 7.43 (m, 2H), 4.17 (q, 2H, J = 7.3), 3.74 (dd, IH, J= 5.5, 7.5), 3.13 (dd, IH, J = 5.5, 13.8), 2.96 (dd, 1H, J = 7.5, 13.8), 1.51 (s, 211),
1.24 (t, 3H, J = 7.3). HRMS: calcd for C12H15F3NO2: 262.1055; found, 262.1054.
Ethyl 2-amino-3-(4-(trifluoromethyl)phenyl)propanoate (B-26): Obtained as an oil (1.23 g, 91 %). !H NMR (CDCI3): 7.58 (d, 2U, J = 8.3), 7.35 (d, 2H, J = 8.3), 4.18 (q, 2H, J = 7.3), 3.74 (dd, IH, J = 5.8, 7.8), 3.14 (dd, IH, J- 5.8, 13.4), 2.96 (dd, IH, J - 7.8, 13.4), 1.50 (s, 2H),
1.25 (t, 3H, J = 7.3). HRMS: calcd for Ci2Hi5F3N02: 262.1055; found, 262.1 129.
Ethyl 2-amino-3-(2-fluorophenyl)propanoate (B-27): Obtained as an oil (2.57 g, 88 %). Ή NMR (CDCI3): 7.23 (m, 2H), 7.06 (m, 2H), 4.17 (q, 2H, J = 7.1), 3.76 (dd, I H, J= 7.9, 5.9), 3.1 1 (dd, IH, J = 13.6, 5.9), 2.93 (dd, IH, J = 13.6, 7.9), 1.24 (t, 3H, J = 7.1). HRMS: calcd for CnH15FN02, 212.1087; found, 212.1089.
Ethyl 2-amino-3-(2,4-difluorophenyl)propanoate (B-28): Obtained as an oil (1.1 1 g, 85 %). !H NMR (CDCI3): 7.19 (m, IH), 6.80 (m, 2H), 4.15 (q, 2H, J = 7.1), 3.70 (dd, IH, J = 7.9, 5.9), 3.04 (dd, IH, J = 13.7, 5.9), 2.89 (dd, 1H, J = 13.7, 7.9), 1.52 (s, 2H), 1.23 (t, 3H, J = 7.1). HRMS: calcd for Ci iHi4F2N02, 230.0993; found, 230.1006.
Ethyl 2-amino-3-(4-chlorophenyl)propanoate (B-29): Obtained as an oil (1.48 g, 73 %). Ή NMR (CDCI3): 7.29 (m, 2H), 7.16 (m, 2H), 4.18 (q, 2H, J = 13), 3.69 (dd, 1H, J = 5.6, 7.5), 3.05 (dd, IH, J= 5.6, 13.7), 2.84 (dd, IH, J= 7.5, 13.7), 1.47 (s(l), 2H), 1.26 (t, 3H, J = ΊΛ). HRMS: calcd for CnH,5ClN02) 228.0791 ; found, 228.0806. Ethyl 2-amino-3-(2-chlorophenyl)propanoate (B-30): Obtained as an oil (3.73 g, 19 % from 2- chlorobenzaldehyde). Ή NMR (CDCK): 7.38 (m, 1H), 7.22 (m, 3H), 4.16 (m, 2H), 3.84 (dd,
J= 8.5, 5.8 Hz, 1H), 3.24 (dd, J= 13.5, 5.8 Hz, 1H), 3.96 (dd, J= 13.5, 8.5 Hz, 1H), 1.23 (t, J = 7.2 Hz, 3H). HRMS: calcd for CnH15ClN02: 228.0791 ; found, 228.0791.
Ethyl 2-amino-3-(4-bromophenyl)propanoate (B-31): Obtained as an oil (0.61 g, 76 %). Ή
NMR (CDCI3): 7.44 (m, 2H), 7.10 (m, 2H), 4.18 (q, 2H, J = 7.3), 3.69 (dd, 1H, J= 5.3, 7.7), 3.04 (dd, 1H, J = 5.3, 13.7), 2.84 dd, 1H, J = 7.7, 13.7), 1.47 (s(l), 2H), 1.26 (t, J = 7.1 Hz, 3H). HRMS: calcd for CnHi5BrN02, 272.0286; found, 272.0296.
Ethyl 2-amino-3-(3-fluorophenyl)propanoate (B-32): Obtained as an oil (4.89 g, 89 %). Ή NMR (CDCI3): 7.27 (m, 1H), 7.01 -6.92 (m, 3H), 4.18 (q, 2H, J= 7.0), 3.72 (dd, 1H, J = 5.4, 7.9), 3.08 (dd, 1H, J = 5.4, 13.7), 2.88 (dd, 1H, J = 7.9, 13.7), 1.49 (s, 2H), 1.26 (t, 3H, J = 7.0). HRMS: calcd for CnH,5FN02: 212.1087; found, 212.1088.
Ethyl 2-amino-3-(4-isopropylphenyl)propanoate hydrochloride (B-33): Obtained as an oil which was immediately treated with a solution of hydrogen chloride in dioxane and concentrated to dryness to give the hydrochloride salt as a white powder (1.9 g, 91 % pure). Ή NMR (DMSO-<4): 8.23 (s, 3H), 7.22 - 7.12 (m, 4H), 4.16 - 4.02 (m, 3H), 3.16 (dd, 1H, J = 13.9, 5.8), 3.01 (dd, 1 H, J = 13.9, 7.8), 2.86 (hept, 1H, J = 6.9), 1.18 (d, 6H, J = 6.9), 1.06 (t, 3H, J= 7.1). HRMS: calcd for C14H22NO2: 236.1651; found, 236.1651.
Ethyl 2-amino-3-(4-cyclopropylphenyl)propanoate (B-34): Obtained as an oil (0.71 g, 80 %). Ή NMR (CDC½): 7.09 (m, 2H), 7.01 (m, 2H), 4.18 (q, 2H, J = 7.0), 3.70 (dd, 1H, J - 5.2, 7.9), 3.06 (dd, 1H, J= 5.2, 13.7), 2.83 (dd, 1H, J= 7.9, 13.7), 1.87 (m, 1H), 1.54 (s, 2H), 1.28 (t, 3H, J = 7.0), 0.95 (m, 2H), 0.68 (m, 2H). HRMS: calcd for C14H20NO2: 234.1494; found, 234.1490.
Ethyl 2-amino-3-(3-cyclopropylphenyl)propanoate (B-35): Obtained as an oil (1.66 g, 89 %). 1H NMR (CDCI3): 7.19 (m, 1H), 6.95 (m, 3H), 4.19 (q, 2H, J = 7.0), 3.71 (dd, 1H, J = 5.2, 7.9), 3.05 (dd, 1 H, J= 5.2, 13.5), 2.83 (dd, 1H, J= 7.9, 13.5), 1.88 (m, 1H), 1.53 (s, 2H), 1.28 (t, 3H, J = 7.0), 0.95 (m, 2H), 0.68 (m, 2H). HRMS: calcd for Ci4H2oN02: 234.1494; found, 234.1496.
Ethyl 2-araino-3-(4-propylphenyl)propanoate hydrochloride (B-36): Obtained as an oil which was immediately treated with a solution of hydrogen chloride in dioxane and concentrated to dryness to give the hydrochloride salt as a white powder (2.46 g, 85 %). 1H NMR (DMSO- d6): 8.59 (bs, 3H), 7.15 (s, 4H), 4.20 (dd, J = 7.5, 5.9 Hz, 1H), 4.10 (m, 2H), 3.16 (dd, J= 7.9,
5.9 Hz, 1H), 3.02 (dd, J = 14.2, 7.9 Hz, 1H), 2.54 (m, 2H), 1.58 (m, 2H), 1.09 (t, J= 7.2 Hz,
3H), 0.89 (t, J= 7.3 Hz, 3H). HRMS: calcd for C14H22N02: 236.1651; found, 236.1659. Ethyl 2-amino-3-(3-propylphenyl)propanoate hydrochloride (B-37): Obtained as an oil which was immediately treated with a solution of hydrogen chloride in dioxane and concentrated to dryness to give the hydrochloride salt as a white powder(1.89 g 90 %). Ή NMR (DMSO-d6): 8.72 (bs, 3H), 7.24 (m, 1H), 7.08 (m, 3H), 4.18 (dd, J= 7.9, 5.6 Hz, 1H), 4.08 (q, J= 7.2 Hz, 2H), 3.21 (dd, J = 13.9, 5.6 Hz, III), 3.03 (dd, J = 13.9, 7.9 Hz, 1H), 2.53 (m, 2H), 1.58 (m, 2H), 1.08 (t, J = 7.2 Hz, 3H), 0.89 (t, J = 7.3 Hz, 3H). HRMS: calcd for CMH22N02: 236.1651; found, 236.1637.
Ethyl 2-amino-3-(5-(trifluoromethyl)furan-2-yl)propanoate (B-38): Obtained as a 95 % pure oil (0.53 g, 93 %). lE NMR (CDC13): 6.66 (dd, 1H, J= 3.3, 1.2), 6.20 - 6.15 (m, 1H), 4.20 - 4.10 (m, 2H), 3.79 - 3.73 (m, 1H), 3.08 (dd, 1H, J = 15.0, 5.4), 2.99 (dd, 1H, J = 15.0, 7.1), 1.60 (bs, 2H), 1.22 (t, 3H, J = 7.1). HRMS: calcd for Ci0Hi3F3NO3! 252.0847; found, 252.0852.
Ethyl 2-amino-3-(5-cyclopropylfuran-2-yl)propanoate (B-39): Obtained as an oil (0.70 g, 88 %). Ή NMR (CDC13): 5.99 (d, J 3.1 Hz, 1H), 5.85 (d, J - 3.1 Hz, I II), 4.20 (q, 2H, J = 7.2), 3.74 (dd, 1 H, J = 7.1 , 5.1), 3.03 (dd, 1 H, J = 14.8, 5.1), 2.93 (dd, 1H, J= 14.8, 7.1), 1.84 (m, 1 H), 1.36 (t, 3H, J = 7.2), 0.84 (m, 2H), 0.73 (m, 2H). HRMS: calcd for C!2H! 7N03,
224.1287; found, 224.1282.
Ethyl 2-amino-3-(4,5-dimethyloxazol-2-yl)propanoate (B-40): Obtained as an oil (0.23 g, 74 %). Ή NMR (CDCb): 4.19 (m, 2H), 3.90 (dd, 1 H, J = 4.5, 8.1), 3.14 (dd, 1H, J= 4.5, 15.0), 2.96 (dd, 1H, J = 8.1, 15.0), 2.27 (s, 3H), 2.05 (s, 3H), 1.84 (s(l), 2H), 1.26 (t, 3H, J = 7.3). HRMS: calcd for C10H17N2O3, 213.1229; found, 213.1230.
Ethyl 2-amino-3-phenylbutanoate (B-41): Obtained as an oil (0.76 g, 88 %) containing a 1/2 mixture of two diastereoisomers. Ή NMR (CDCI3): 7.39-7.21 (m, 5H), 4.20 (q, 1.3H, J = 7.1), 4.20 (q, 0.7H, J = 7.1), 3.62 (d, 0.3H, J = 6.0), 3.56 (d, 0.7H, J = 7.2), 3.18 (m, 0.3H), 3.12 (m, 0.3H), 1.44 (s, 2H), 1.36 (d, 2H, J= 7.2), 1.34 (d, 1 H, J= 6.0), 1.29 (t, 2H, J= 7.1), 1.16 (t, 1H, J= 7.1). HRMS: calcd for C!2HI8N02, 208.1338; found, 208.1331.
Preparation of 2-AmIno-4-phenyIbutanoate (B-42): Ethyl 5-phenyl-4,5-dihydroisoxazole- 3-carboxylate (WO20100142801 , 2010) (0.47 g, 2.14 mmol), ammonium formate (2.7 g, 21.4 mmol) and 10 % palladium over charcoal (0.1 1 g, 0.10 mmol) were heated to reflux in ethanol (50 mL) fro one hour. This was filtered, the isoluble washed with a small amount of ethanol and 37 % hydrochloric acid (1.6 mL, 13.6 mmol) was added before adding powdered zinc (0.42 g, 6.3 mmol) by portions. This was stirred for 90 mmn, the resulting solution was diluted in water made basic with 22 % ammonia and extracted with ethyl acetate. The organic layer was washed wirth water, brine, dried over sodium carbonate and concentrated to dryness to yield the amino ester as an oil (0.32 g, 72 %). Ή NMR (CDC13): 7.31 (m, 2H), 7.22 (m, 3H), 4.19 (m, 2H), 3.46 (dd, 1H, J= 5.3, 7.9), 2.76 (m, 2H), 2.07 (m, 1 H), 1.87 (m, 1H), 1.65 (s, 2H), 1.30 (t, 3H, J = 7.1).
Preparation of ethyl 2-amino-3-(l,3-dioxolan-2-yl)propanoate (B-43): Step 1 : the previously described (J. Org. Chem., 2006, 360) diethyl 2-((l,3-dioxolan-2- yl)methyl)malonate (7.8 g, 0.317 moi) was dissolved in ethanol (40 mL) and potassium hydroxide (1.78 g, 0.0317 moi) dissolved in ethanol (60 mL) was added drop wise. The solution was stirred overnight at room temperature and then briefly heated to reflux. This was concentrated, dissolved in water which was made more basic with 22 % ammonia. The aqueous phase was washed with dichloromethane, made acid with 37 % hydrochloric acid, extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield pure 2-((l ,3-dioxolan-2-yl)methyl)-3- ethoxy-3-oxopropanoic acid (3.85 g, 55 %). Ή NMR (CDC13): 5.05 (t, 1 H, J = 3.7), 4.24 (q, 2H, J= 7.1), 3.98 (m, 2H), 3.82 (m, 2H), 3.64 (t, 1 H, J = 6.9), 2.40 (m, 2H), 1.30 (t, 311, J = 7.1). Step 2: Under argon, this compound (3.85 g, 0.0176 moi) was dissolved in toluene (50 mL, dried over 4A molecular). Triethylamine (2.95 mL, 0.021 moi) was added and then diphenylphosphoryl azide (4.18 mL, 0.021 moi). This was stirred at 80 °C for lh30, the toluene was removed under vacuum and the resulting oil repeatedly extracted with cyclohexane. Concentration to dryness of this extract led to an oil which was stirred at room temperature in 4 % hydrochloric acid (50 mL) overnight. The resulting solution was diluted in more acid, washed with ethyl acetate, the aqueous phase was made basic with 22 % ammonia, extracted with ethyl acetate, and this organic layer was washed with water brine, dried over sodium carbonate and concentrated to dryness to yield the amino ester as an oil (0.14 g, 16%). Ή NMR (CDCI3): 5.05 (dd, 1H, J= 4.3, 5.0), 4.0 (m, 2H), 3.87 (m, 2H), 3.66 (dd, 1H, J = 4.6, 8.2), 2.15 (ddd, 1 H, J = 4.6, 5.0, 14.2), 1.94 (ddd, 1H, J = 4.3, 8.2, 14.2), 1.57 (s, 2H), 1.29 (t, 3H, J= 7.1). HEMS: calcd for C8H16N04, 190.1079; found, 190.1061.
Synthesis of piperazin-2-ones E, representative preparation of (3S,5S) and (3S,5R)-3- benzyl-5-phenylpiperazin-2-ones. Step a, preparation of ethyl (2 -nitro-1 -phenyl ethyl)-L- phenylalaninate: 2-nitrovinylbenzene (3.78 g, 0.025 moi) was added to a freshly extracted free base of L-phenylalanine ethyl ester (4.9 g, 0.025 moi). Upon stirring on a rotatory evaporator at room temperature the suspension homogenised in about 10 minutes to give the 1,4 adduct C. Step b, preparation of ethyl (2-amino- 1 -phenyl ethyl )phenylalaninate: this oil was dispersed in a cold solution of dioxane (100 mL) and 37% hydrochloric acid (38.7 mL, 0.33 moi). Zinc dust (7.45 g, 0.11 g, < 10 μπι) was added portion-wise in the course of 10 minutes. The temperature was then allowed to rise to room temperature and the suspension was stirred for 2 hours. This was diluted in water, made basic with an excess of 22 % ammonia and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium carbonate and concentrated to dryness to give the crude compound D as an oil Step c, preparation of 3-benzyl-5-phenylpiperazin-2-ones E: under an argon atmosphere, this oil was heated at 140 °C for 3 hours. The ethanol was removed under vacuum and the resulting solid was subjected to purification procedures as described below. Note: whne separateted, the structure attribution of these pairs of diastereoisomers can be made by checking for the existence (or not) of a nOe effect between H-3 and H-5.
(3S,5R.)-3-benzyl-5-phenylpiperazin-2-one (E-l): The crude mixture of diastereoisomers was dispersed in boiling cyclohexane to remove unreacted L-phenylalanine ester and the resulting solid purified by a chromatography over silica gel (dichloromethane / ethanol 96/4 to 95/5) to yield this compound as a white solid (2.05 g, 35 %). ¾ NMR (CDCI3): 7.42 ~~ 7.29 (m, 9H), 7.26 - 7.20 (m, 1H), 6.27 (s, I H), 4.06 (dd, 1 H, J = 9.7, 4,7), 3.84 (dt, I H, J= 10.1, 3.4), 3.60 (dd, IH, J = 13.6, 3.1), 3.41 - 3.25 (m, 2H), 2.91 (dd, 1H, J - 13.6, 10.1), 1.78 (s, III). HRMS: calcd for C!7Hi9N20, 267.1497; found: 267.1459.
(3S,5S)-3-benzyl-5-phenylpiperazin-2-one (E-2): A second chromatographic fraction gave this isomer as a white solid (1.29 g, 22 %). Ή NMR (CDC13): 7.46 - 7.18 (m, 10H), 6.44 (s, IH), 4.27 (dd, IH, J = 9.6, 4.0), 3.89 (dd, IH, J = 10.7, 3.6), 3.54 - 3.44 (m, IH), 3.41 (dt, IH, J = 1 1.5, 4.0), 3.32 (dd, 1H, J = 13.8, 3.6), 3.18 (dd, 1H, J = 13.8, 10.7), 1.79 (s, IH). HRMS: calcd for C,7Hi N20, 267.1497; found: 267.1435.
3-Benzyl-5-(p-tolyl)piperazin-2-one (E-3): This compound was obtained as a crude mixture of the two diastereoisomers (5.40 g) by dispersing the resulting solid in boiling cyclohexane and filtration at 20 °C and was used directly, in the aromatization step. HRMS: calcd for C,8H20N2O, 281.1654; found: 281.1649.
3-Benzyl-5-(m-tolyl)piperazin-2-one (E-4): An analytical sample of a mixture of the the two diastereoisomers was obtained by a recrystallization in cyclohexane but most of the solid and the filtrate were used directly in the in the aromatization step. HRMS: calcd for Ci8H2oN20, 281.1654; found: 281.1645.
Cis-3-(2-methylbenzyl)-5-phenylpiperazin-2-one (E-5): This compound was obtained as a white powder (4.04 g, 26 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a dispersion in boiling cyclohexane. Ή NMR (CDC13): 7.40 - 7.27 (m, 6H), 7.14 (m, 3H), 6.58 (s (br), IH), 4.03 (dd, IH, J ~ 4.1 , 10.5), 3.82 (d (br), IB, J = 10.5), 3.68 (dd, 1H, J = 2.7, 14.0), 3.36 (m, 2H), 2.92 (dd, 1H, J = 10.8, 13.7), 2.45 (s, 3H),
I .77 (s, 1H). HRMS: calcd for€18Η2ιΝ205 281.1654; found: 281.1652.
Trans-3-(2-methylbenzyl)-5-phenylpiperazin-2-one (E-6): This compound was obtained as a white powder (2.77 g, 17 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a dispersion in boiling cyclohexane. 5H NMR (CDC13): 7.41 ~~ 7.30 (m, 5H), 7.14 (m, 4H), 6.80 (s (br), 1H), 4.35 (dd, 1H, J = 5.0» 9.8), 3.89 (dd, 1H, J = 3.3,
I I .2), 3.43 (m, 2H), 3.39 (dd, 1H, J = 3.3, 13.9), 3.16 (dd, 1H, J = 11.2, 13.9), 2.40 (s, 3H), 1.76 (s, 1H). HRMS: calcd for Ci8H2iN20, 281.1654; found: 281.1652.
Cis-3-(3-methylbenzyl)-5-phenylpiperazin-2-one (E-7): This compound was obtained as a white powder (3.70 g, 26 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a dispersion in boiling cyclohexane. Ή NMR (CDC13): 7.40 - 7.28 (m, 5H), 7.20 (m, 1H), 7.13 (m, 2H), 7.04 (m, 1H), 6.72 (s (br), 1 H), 4.05 (dd, 1H, J = 5.4, 9.5), 3.83 (d (br), 1H, J = 10.4), 3.58 (dd, 1H, J = 3.0, 13.6), 3.35 (m, 2H), 2.86 (dd, 1H, J = 10.4, 13.6), 2.34 (s, 3H), 1.80 (s, 1H). HRMS: calcd for C! 8H21N20, 281.1654; found: 281.1649.
Trans-3-(3-methylbenzyl)-5-phenylpiperazin-2-one (E-8): This compound was obtained as a white powder (2.73 g, 19 %) after a chromatography over silica gel (dichloromethane / ethanol 98/2 to 95/5) and a recrystallization in cyclohexane. !H NMR (CDC13): 7.39 - 7.30 (m, 5H), 7.19 (m, 1H), 7.05 (m, 3H), 6.81 (s (br), 1H), 4.26 (dd, 1 H, J = 4.0, 9.9), 3.87 (dd, 1 H, .7 = 3.8, 10.7), 3.45 (m, 2Π), 3.28 (dd, 1H, J = 3.5, 13.7), 3.14 (dd, 1 H, J = 10.7, 13.7), 2.31 (s, 3H), 1.81 (s, 1H). HRMS: calcd for CigH21N20, 281.1654; found: 281.1656.
(3S,5R)-3-benzyl-5-(2-methoxyphenyl)piperazin-2-one (E-9): This isomer was obtained as a glass (1.06 g, 25 %) after two chromatography over silica gel (dichloromethane - ethanol 96/4 to 95/5) and (cyclohexane - ethyl acetate 1/2 to 1/3). Ή NMR (CDC13): 7.40-7.22 (m, 7H), 6.98 (m, 1H), 6.84 (m, 1 H), 6.69 (s (br), 1H), 4.39 (dd, 1H, J = 3.7, 10.7), 3.74 (s, 3H), 3.85 (dd, 1H, J = 3.2, 9.2), 3.53 (dd, 1H, J = 3.5, 13.7), 3.44 (ddd, 1H, 7 = 4.0, 8.0, 11.0), 3.28 (t, 1H, 7 = 11.0), 3.01 (dd, 1H, 7 = 8.3, 13.7), 1.90 (s (br), 1H). HRMS: calcd for C18¾iN20: 297.1603, found: 297.1591.
(3S,5S)-3-benzyl-5-(2-methoxyphenyl)piperazin-2-one (E-10): This isomer was obtained as a white solid (0.52 g, 12 %) after two chromatography over silica gel (dichloromethane - ethanol 96/4 to 95) and (ethyl acetate - ethanol 1/0 to 99/1). lll NMR (CDC13): 7.36-7.20 (m, 7H), 6.94 (m, 1 H), 6.88 (m, 1H), 6.65 (s (br), 1 H), 4.64 (dd, 1H, J = 4.2, 8.1), 3.77 (s, 3H), 3.71 (dd, 1H, J= 3.3, 10.8), 3.59 (m, 1 H), 3.47 (ddd, 1H, 7= 1.0, 11.2), 3.36 (dd, 1H, J= 3.3, 13.7), 3.09 (dd, 1H, J= 10.8, 13.7), 2.08 (s (br), IH). HRMS: calcd for Ci8H2iN20: 297.1603, found: 297.1589.
(3S,5R)-3-benzyl-5-(3-methoxyphenyl)piperazin-2-one (E-l 1): This isomer was obtained as a glass (1.33 g, 30 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (cyclohexane - ethyl acetate 1/4). 1H NMR (CDC13): 7.34-7.20 (m, 6H), 6.94 (m, 2H), 6.83 (m, 1H), 6.72 (s (br), IH), 4.02 (dd, 1H, J = 6.3, 8.7), 3.83 (m, 1H), 3.81 (s, 3H), 3.58 (dd, IH, J = 3.3, 13.4), 3.34 (m, 2H), 2.91 (dd, IH, J = 10.3, 13.4), 1.90 (s (br), IH). HRMS: calcd for CI8H2,N20: 297.1603, found: 297.1582.
(3S,5S)-3-benzyl-5-(3-methoxyphenyl)piperazin-2-one (E-12): This isomer was obtained as a glass (0.84 g, 19 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (ethyl acetate - ethanol 1/0 to 99/1). !H NMR (CDC13): 7.33-7.22 (m, 6H), 6.94 (m, 2H), 6.85 (m, IH), 6.76 (s (br), IB), 4.22 (dd, IH, J = 4.1, 9.5), 3.88 (dd, IH, J = 3.6, 10.6), 3.80 (s, 3H), 3.45 (m, 2H), 3.31 (dd, IH, J = 3.6, 13.8), 3.16 (dd, IH, J = 10.7, 13.8), 1.81 (s (br), IH). HRMS: calcd for C!8H2iN20: 297.1603, found: 297.1584.
(3S,5R)-3-benzyl-5-(4-methoxyphenyl)piperazin-2-one (E-l 3): This isomer was obtained as a glass (1.37 g, 27 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (cyclohexane - ethyl acetate 1/4). Ή NMR (CDC13): 7.28 (m, 7H), 6.87 (m, 2H), 6.38 (s (br), IH), 3.99 (dd, IH, J 4.3, 10.3), 3.82 (m, IH), 3.80 (s, 3H), 3.58 (dd, IH, J - 3.8, 13.6), 3.31 (m, 2H), 2.89 (dd, IH, J - 10.2, 13.6), 1.90 (s (br), IH). HRMS: calcd for C18.H21N2O: 297.1603, found: 297.1585.
(3S,5S)-3-benzyl-5-(4-methoxyphenyl)piperazin-2-one (E-14):This isomer was obtained as a white powder (0.81 g, 16 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (ethyl acetate - ethanol 1/0 to 99/1). !H NMR (CDC13): 7.28 (m, 7H), 6.81 (m, 2H), 6.73 (s (br), IH), 4.19 (dd, IH, J = 4.0, 9.8), 3.86 (dd, 1 H, J = 3.6, 10.6), 3.80 (s, 3H), 3.44 (m, IH), 3.31 (m, 2H), 3.36 (td, IH, J = 3.8, 11.5), 3.30 (dd, 1H, J = 3.8, 13.9), 3.17 (dd, IH, J= 10.6, 13.9), 1.85 (s (br), I H). HRMS: calcd for C,8H2,N20: 297.1603, found: 297.1533.
(3S,5R)-3-benzyl-5-(2-fiuorophenyl)piperazin-2-one (E-l 5): This isomer was obtained as a white powder (4.06 g, 39 %) after two chromatography over silica gel (dichloromethane - ethanol 96/4 to 94/6) and (cyclohexane - ethyl acetate 2/3 to 1/3) followed by a dispersion in boiling cyclohexane and extensive drying at 70 °C under vacuum. Ή NMR (CDCI3): 7.49 (m, IH), 7.31 (m, 4H), 7.26 (m, 2H), 7.16 (m, IH), 7.02 (m, IH), 6.65 (s (br), IH), 4.42 (m, IH), 3.87 (m, IH), 3.57 (dd, IH, J= 3.3, 13.8), 3.29 (t, I H, J = 10.9), 2.95 (dd, IH, J = 9.1, 13.8), 1.73 (s (br), IH). HRMS: calcd for C!7H,gFN20: 285.1403, found: 285.1391. (3S,5S)-3-benzyl-5-(2-fluorophenyl)piperazin-2-one (E-16): This isomer was obtained as a white powder (2.16 g, 20 %) after a chromatography over silica gel (dichloromethane - ethanol 96/4 to 94/6). Ή NMR (CDC13): 7.44 (m, 1H), 7.27 (m, 6H), 7.13 (m, 1H), 7.08 (m, 1 H), 6.74 (s (br), 1 H), 4.65 (dd, 1H, J = 4.3, 9.1 ), 3.84 (dd, 1H, J = 3.5, 10.8), 3.48 (m, 2H), 3.34 (dd, 1 H, J = 3.8, 13.7), 3.15 (dd, 1H, J = 10.7, 13.7), 1.78 (s (br), 1H). HRMS: calcd for C17Hi8FN20: 285.1403, found: 285.1367.
3-benzyl-5-(2,6-difluorophenyl)piperazin-2-one (E- 17): This compound was obtained as a 1/1 mixture of diastereoisomers after a recrystallization in cyclohexane (1.97 g, 62 %). HRMS: calcd for C17H!7F2N20: 303.1309, found: 303.1323.
(3S,5R)-3-benzyl-5-(4-fluorophenyl)piperazin-2-one (E-18): This isomer was obtained as a white powder (1.26 g, 29 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (cyclohexane - ethyl acetate 1/2 to 1/3). Ή NMR (CDC13): 7.28 (m, 7H), 7.01 (m, 2H), 6.61 (s (br), 1 H), 4.05 (t, 1H, J = 7.3), 3.82 (d, 1 H, J = 9.3), 3.58 (dd, 1 H, J = 3.0, 13.6), 3.31 (m, 211), 2.89 (dd, 1H, J = 9.8, 13.6), 1.72 (s (br), 1H). HRMS: calcd for Ci7H18FN20: 285.1403, found: 285.1342.
(3S,5S)-3-benzyl-5-(4-fluorophenyl)piperazin-2-one (E- 19): This isomer was obtained as a white powder (1.26 g, 29 %) after two chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and (ethyl acetate - ethanol 99/1). lH NMR (CDCI3): 7.29 (m, 7H), 7.05 (m, 2H), 6.79 (s (br), 111), 4.21 (dd, 1H, J = 4.5, 9.5), 3.86 (dd, 1H, J = 3.7, 10.6), 3.40 (m, 2H), 3.16 (dd, 1 H, J = 10.7, 13.9), 1.74 (s (br), 1H). HRMS: calcd for C17H! 8FN20: 285.1403, found: 285.1315.
3-benzyl-5-(3-fluorophenyl)piperazin-2-one (E-20): This mixture of isomers was obtained as an oil, after washing it with cyclohexane to remove unreacted ethyl phenylalanine ester, and used directly in the aromatization step.
(3S,5R)-3-benzyl-5-(4-(benzyloxy)phenyl)piperazin-2-one (E-21): This isomer was obtained as a white powder (0.92 g, 17 %) after two chromatography over silica gel (dichloromethane - ethanol 95/5 to 92/8) and (cyclohexane - ethyl acetate 1/2) and a recrystallization in cyclohexane. Ή NMR (CDCI3): 7.45 - 7.21 (m, 12H), 6.94 (m, 2H), 6.47 (s (br), 1 H), 5.06 (s, 2H), 4.00 (dd, 1H, J = 4.3, 10.1), 3.83 (dd, 1H, J = 3.0, 10.1), 3.59 (dd, 1H, J = 3.0, 13.6), 3.32 (m, 2H), 2.90 (dd, 1H, J = 10.1, 13.6), 1.73 (s (br), 1H). HRMS: calcd for C25H25N2O3: 373.1916, found: 373.1932.
(3S,5S)-3-benzyl-5-(4-(benzyloxy)phenyl)piperazin-2-one (E-22): This isomer was obtained as a white powder (0.59 g, 1 1 %) after two chromatography over silica gel (dichloromethane - ethanol 95/5 to 92/8) and (ethyl acetate - ethanol 1/0 to 99/1). Ή NMR (CDCI3): 7.45 - 7.23 (m, 12H), 6.96 (m, 2H), 6.67 (s (br), 1 H), 5.07 (s, 2H), 4.21 (dd, 1H, J = 4.0, 9.6), 3.85 (dd, !H, J = 3.5, 10.7), 3.40 (m, 2H), 3.31 (dd, 1H, J = 3.5, 13.8), 3.18 (dd, 1H, J = 10.7, 13.7), 1.76 (s (br), 1H). HRMS: calcd for C25H25N203: 373.1916, found: 373.1907.
(3S,5R)-3-benzyl-5-(4-(benzyloxy)-3-fluorophenyl)piperazin-2-one (E-23): This isomer was obtained as a powder (2.47 g, 54 %, 90 % pure) after a chromatography over silica gel (dichloromethane - ethanol 98/2 to 95/5). Ή NMR (CDC13): 7.44 ~ 7.21 (m, 10H), 7.15 (m, 1H), 7.01 (m, 1 H), 6.95 (m, IH), 6.19 (s (br), 1H), 5.13 (s, 2H), 3.96 (m, IH), 3.81 (dd, HI, J = 3.0, 10.1 ), 3.58 (dd, 1H, J = 3.0, 13.8), 3.28 (m, 2H), 2.88 (dd, IH, J= 10.2, 13.8), 1.71 (s (br), IH). HRMS: calcd for C24H25FN203: 391.1822, found: 391.1828.
(3S,5S)-3-Benzyl-5-(4-(benzyloxy)-3-fluorophenyl)piperazin-2-one (E-24): This isomer was obtained as as a powder (0.5 g, 1 1 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2 to 95/5). Ή NMR (CDC13): 7.45 - 7.22 (m, 10H), 7.13 (m, IH), 7.02 (m, IH), 6.96 (m, IH), 6.19 (s (br), I H), 5.14 (s, 2H), 4.15 (dd, IH, J = 4.1, 9.3), 3.86 (dd, III, J - 3.6, 10.4), 3.45 - 3.33 (m, 2H), 3.29 (dd, I H, J = 4.1 , 14.4), 3.16 (dd, IH, J = 10.8, 13.8), 1.71 (s (br), IH). HRMS: calcd for C24H25FN2O3: 391.1822, found: 391.1828.
Cis-3-(2-fluorobenzyl)-5-phenylpiperazin-2-one (E-25): This racemic isomer was obtained from B-27 as a white powder (2.85 g, 31 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and a dispersion in boiling cyclohexane. Ή NMR (CDC13): 7.35 (m, 6H), 7.21 (m, IH), 7.07 (m, 2H), 6.60 (s (br), IH), 4.08 (dd, IH, J = 6.4, 8.3), 3.92 (dd, 1 H, J 3.1 , 9.6), 3.65 (dd, \ , J = 3.0, 14.0), 3.34 (m, 2H), 2.97 (dd, IH, J = 9.6, 14.0), 1.78 (s (br), I H). HRMS: calcd for C17H!8FN20: 285.1403, found: 285.1412.
Trans-3-(2-fluorobenzyl)-5-phenylpiperazin-2-one (E-26): This racemic isomer was obtained from B-27 as a white powder (2.15 g, 23 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3 to 95/5) and a recrystallization of a sample in cyclohexane for analytical purposes. Ή NMR (CDC13): 7.40-7.26 (m, 6H), 7.21 (m, IH), 7.09 (m, I H), 7.03 (m, IH), 6.92 (s (br), IH), 4.33 (dd, 1 H, J = 3.9, 9.8), 3.92 (dd, 1 H, J = 3.9, 10.6), 3.51 - 3.33 (m, 311), 3.27 (dd, IH, J = 4.0, 14.0), 1.72 (s (br), IH). HRMS: calcd for C,7Hi8FN20: 285.1403, found: 285.1410.
3-(2-Chlorobenzyl)-5-phenylpiperazin-2-one (E-27): This compound was obtained from B-30 as a white powder containing a mixture of the two diastereoisomers after a recrystallization in cyclohexane (2.21 g, 45 %). Ή NMR (CDCI3): 7.42-7.29 (m, 7H), 7.25-7.14 (m, 2H), 6.85 (bs, 0.4H), 6.69 (bs, 0.6H), 4.37 (dd, J= 9.8, 4.2 Hz, 0.4H), 4.10-3.98 (m, 1.4H), 3.83 (dd, J= 13.8, 3.4 Hz, 0.6H), 3.51-3.30 (m, 3H), 3.02 (dd, J = 13.8, 9.8 Hz, 0.6H). HRMS: calcd for Cnl½ClN20: 301.1 108; found, 301.1 1 17. Cis-3-(2-methoxybenzyl)-5-phenylpiperazin-2-one (E-28): This isomer was obtained as a powder (4.03 g, still containing 5 % of the aminoester) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5). Ή NMR (CDC13): 7.39-7.27 (m, 6H), 7.21 (m, IH), 6.89 (m, 2H), 6.38 (s (br), 1H), 4.05 (dd, 1H, J = 5.3, 93), 3.92 (m, 1 H), 3.84 (s, 3H), 3.75 (dd, 1H, J= 3.3, 13.7), 3.35 (m, 2H), 2.88 (dd, 1H, J = 10.3, 13.7), 1.81 (s (br), 1H). HRMS: calcd for C!8H2!N20: 297.1603, found: 297.1607.
Trans-3-(2-methoxybenzyl)-5-phenylpiperazin-2-one (E-29): This isomer was obtained as a powder (2.23 g, 24 %) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5). Ή NMR (CDC13): 7.39-7.30 (m, 5H), 7.21 (m, 2H), 6.92 (m, 1 H), 6.82 (m, I H), 6.70 (s (br), IH), 4.36 (dd, 1 H, J = 3.9, 9.9), 3.97 (dd, IH, J = 3.9, 10.7), 3.68 (s, 3H), 3.46-
3.32 (m, 4H), 3.26 (dd, IH, J = 3.8, 13.5), 1.83 (s (br), IH). HRMS: calcd for Ci8H2iN20: 297.1603, found: 297.1604.
Cis-3-(3-fluorobenzyl)-5-phenylpiperazin-2-one (E-30): This isomer was obtained as a powder (2.25 g, 13 %) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5) and a recrystallization in a mixture of toluene and cyclohexane. Ή NMR (CDC13):
7.40-7.24 (m, 6H), 7.10 (m, IH), 7.05 (m, IH), 6.92 (m, IH), 6.62 (s (br), IH), 4.07 (m, IH), 3.85 (dd, IH, J= 2.9, 10.0), 3.55 (dd, 1H, J= 2.9, 13.5), 3.34 (m, 2H), 2.93 (dd, IH, J= 10.0, 13.5), 1.74 (s (br), IH). HRMS: calcd for Ci7H19FN20: 285.1403, found: 285.1400.
Trans-3-(3-fluorobenzyl)-5-phenylpiperazin-2-one (E-31): This isomer was obtained as a powder (2.49 g, 15 %) after a chromatography over silica gel (dichloromethane - ethanol 97.5/2.5). Ή NMR (CDCI3): 7.35 (m, 5H), 7.26 (m, 3H), 6.70 (s (br), IH), 4.21 (dd, IH, J = 4.2, 9.3 Hz, IH), 3.87 (dd, IH, J= 3.8, 10.3), 3.54-3.41 (m, 2H), 3.30 (dd, IH, J= 3.8, 14.0), 3.30 (dd, I H, J - 10.3, 14.0), 1.77 (s (br), IH). HRMS: calcd for C17H19FN2O: 285.1403, found: 285.1409.
3-(4-fluorobenzyl)-5-phenylpiperazin-2-one (E-32): This compound was obtained as white powder (0.9 g, 59 % from phenylnitrostyrenc), as a mixture of diasiereoisomers after a recrystallization in cyclohexane. HRMS: calcd for Ci7Hi8FN20: 285.1403, found: 285.1408. (3S,5S)-3-methyl-5-phenylpiperazin-2-one (E-33): This isomer was obtained as a white powder (0.2 g, 12 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3 to 9/1) and a recrystallization in a toluene - cyclohexane mixture. Note: by a simple recrystallization of the crude reaction product, a far better overall yield (7.49 g, 47 %) of a 1/1 mixture of these isomers was obtained in two batches. Ή NMR (CDCI3): 7.37 (m, 5H), 6.47 (s, IH), 4.16 (dd, 1H, J = 4.2, 10.3), 3.75 (d, HI, J - 6.9), 3.41 (m, 2H), 1.46 (d, 3H, J= 6.9). HRMS calcd for C11H14N2O + H: 191.1184 Found: 191.1109. (3S,5R)-3-methyl-5-phenylpiperazin-2-one (E-34): This isomer was obtained as a white powder (0.19 g, 11 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3 to 9/1) and a recrystallization in a toluene - cyclohexane mixture. Note: by simply recrystaliizing the crade reaction product, a far better yield (7.49 g, 47 %) of a 1/1 mixture of these isomers was obtained in two recrystallization batches. !H NMR (CDC13): 7.38 (m, 5H), 6.86 (s, 1H), 4.29 (dd, 1H, J= 4.2, 10.3), 3.78 (q, 1H, J = 7.0), 3.47 (m, 2H), 1.51 (d, 3H, J = 7.0). HRMS calcd for C! iH14N20 + H: 191.1 184 Found: 191.1 138.
3-Benzyl-6-methyl-5-phenylpiperazin-2-one (E-35): Mixture of various diastereoisomers of this compound were obtained after removal of unreacted phenylalanine ethyl ester under high vacuum at 180 °C and a chromatography over silica gel (dichloromethane - methanol 98/2 to 95/5). One of these fractions could be recristallized in cyclohexane to give the single diastereoisomer (0.8 g, 7 %) described below (with an undetermined configuration). In any case, this compound as well, as the other fractions containing different diastereoisomers, were used in the next step. Ή NMR (CDCI3): 7.38-7.28 (m, 7H), 7.24-7.20 (m, 3H), 6.62 (s, I H), 3.90-3.87 (dd, 1H, J = 10.2, 3.8), 3.70-3.63 (m, 2H), 3.30-3.16 (m, 2H), 1.73 (s, 1H), 1.03- 0.98 (m, 3H). HRMS: calcd for Ci8H2iN20: 281.1654, found: 281.1659.
2-Benzyl-l ,4a,5,10b-tetrahydro-2H-chromeno[3,4-b]pyrazin-3(4H)-one (E-36): This compound was prepared as described above from 3 -nitrc-2H-chromene (Synthesis, 1984, 348; Bioorg. Med. Chem., 2011, 5420) but running the addition step at 0°C overnight and obtained, upon refluxing the resulting solid in cyclohexane, as an inseparable mixture containing one major diastereoisomer (4.75 g, 76 %). Ή NMR (CDCI3): 7.38 - 7.29 (m, 16H), 7.20 (m, IH), 7.02 (s (br), IH), 6.97 (m, IH), 6.85 (m, IH), 4.31 (dd, IH, J = 4.3, 10.3), 3.99 (m, 2H), 3.90 (dd, III, J - 3.8, 10.0), 3.68 (ddd, IH, J - 3.9, 9.6, 1 1.1), 3.30 (dd, 1 H, J = 3.9, 13.6), 3.12 (dd, 1 H, J = 10.0, 13.6), 1.79 (s (br), IH). HRMS: calcd for Ci8H19N202: 295.1447, found: 295.1402.
General procedure for the synthesis of pyrazin-2-ols H using sulfur. The considered piperazin-2-one (0.01 1 mol) and sulfur (0.72 g, 0.0225 mol) were heated to reflux in 1 ,3- dichlorobenzene (40 mL) for 10 hours. This was concentrated to dryness and the residue purified as described below.
3-Methyl-5-phenylpyrazin-2-ol (H-l ): Obtained from E-33 and/or E-34 as a beige powder (1.33 g, 69 %) after a chromatography over silica gel (cyclohexane / ethyl acetate 1 :2). Ή NMR (DMSO- ) 12.31 (s, IH), 7.83 (m, 3H), 7.39 (m, 2H), 7.28 (m, IH), 2.36 (s, 3H). HRMS: calcd for C, ,HuN20: 187.0871 ; found, 187.0808. 3-(2-Fluorobenzyl)-5-phenylpyrazin-2-ol (H-2): Obtained from E-25 and/or E-26 as a white powder (3.66 g, 88 %) after a chromatography over silica gel (dichloromethane / ethanol 97.7:2.5). TI NMR (DMSO-^) 12.45 (s, IH), 7.89 (s, 1H), 7.73 (m, 2H), 7.38-7.22 (m, 5H), 7.19-7.12 (m, 2H), 4.12 (s, 2H). HRMS: calcd for Ci7Hi4FN20: 281.1090; found, 281.1087. 3-(2-Chlorobenzyl)-5-phenylpyrazin-2-ol (H-3): Obtained from E-27 as a powder (2.06 g, 68 %) after a chromatography over silica gel (dichloromethane / ethanol 98:2) and a dispersion in a boiling mixture of toluene and cyclohexane. !H NMR (DMSO): 12.47 (bs, 1H), 7.90 (s, 1H), 7.70 (m, 2H), 7.46 (m, 1H), 7.32 (m, 5H), 7.25 (m, 1H), 4.22 (s, 2H). HRMS: calcd for Ci7H14ClN20: 297.0795; found, 297.0795.
3 -(2 -Methoxybenzyl)-5-phenylpyrazin-2 -ol (H-4): Obtained from (E-28) and/or (E-29) as a white powder (1.7 g, 30 %) after a chromatography over silica gel (dichloromethane / ethanol 98.5:1.5 to 97.5:2.5) and a dispersion in a boiling mixture of cyclohexane and toluene. !H NMR (DMSO- ) 12.35 (s (br), 1H), 7.86 (s, 1H), 7.73 (m, 2H), 7.34 (m, 2H), 7.23 (m, 2H), 7.1 1 (m, IB), 6.98 (m, 1H), 6.87 (m, 1H), 4.04 (s, 2H), 3.75 (s, 3H). HRMS: caled for Q8M17N2O2: 293.1290; found, 293.1280.
3-(3-Fluorobenzyl)-5-phenylpyrazin-2-ol (H-5): Obtained from E-30 and/or E-31 as a white powder (3.92 g, 88 %) after a chromatography over silica gel (dichloromethane / ethanol 98:2). A sample was recrystallized in a mixture of cyclohexane and toluene for analytical purposes. Ή NMR (DMSO- ) 12.45 (s (br), 1H), 7.89 (s, 1 H), 7.82 (m, 2H), 7.41-7.26 (m, 4H), 7.17 (m, 2H), 7.04 (m, 1H), 4.10 (s, 2H). HRMS: calcd for C17H14FN2O: 281.1090; found, 281.1650.
3-(4-Fluorobenzyl)-5-phenylpyrazin-2-ol (H-6): This crude compound was obtained from E- 32 after evaporation to dryness and was directly used in the chlorination step without any purification.
3-Benzyl-5-phenylpyrazin-2-ol (H-7): This compound was obtained from E-l and/or E-2 as a white powder (2.28 g, 73 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2 to 97/3). !H NMR (DMSQ-</6) 12.41 (s, IH), 7.85 (m, 3H), 7.40 (m, 4H), 7.30
(m, 3H), 7.20 (m, 1H), 4.07 (s, 2H). HRMS: calcd for C17H15N2O: 263.1184; found, 263.11 18.
3-Benzyl-5-(p-tolyl)pyrazin-2-ol (H-8): A pure sample (1.48 g) of this compound (obtained from E-3) was isolated as a white solid by a dispersion of the crude mixture in dichloromethane, the concentrated filtrate and most of this solid were used directly in the next step. !H NMR (DMSO- ) 12.35 (s, IH), 7.82 (s, IH), 7.72 (m, 2H), 7.40-7.17 (m, 7H), 4.06 (s, 2H), 2.30 (s, 3H). HRMS: calcd for C18H16N2ONa: 299.1160; found, 299.1177. 3-Benzyl-5-(m-tolyl)pyrazin-2-ol (H-9): A crade fraction was obtained from E-4 and isolated by a filtration of the resulting precipitate and washing with toluene. Am analytical sample was obtained from a recrystallization in toluene of the concentrated filtrate. Ή NMR (DMSO-i 6) 12.35 (s, 1H), 7.85 (s, 1H), 7.66 (m, 1H), 7.62 (m, 2H), 7.33-7.17 (m, 5H), 7.08 (m, 1 H), 4.07 (s, 2H), 2.33 (s, 3H). HEMS: calcd for C,8H16N2ONa: 299.1 160; found, 299.1 172.
3-(2-Methylbenzyl)-5-phenylpyrazin-2-ol (H-10): This compound was obtained from E-5 and/or E-6 as a powder (5.65 g, 88 %) after a chromatography over silica gel (dichloromethane - ethanol 99/1 - 98/2). Ή NMR (DMSO-</6) 12.40 (s, 1 H), 7.87 (s, 1H), 7.77 (m, 1H), 7.37 (m, 2H), 7.24 (m, 2H), 7.16 (m, 1H), 7.10 (m, 1H), 4.08 (s, 2H), 2.37 (s, 3H). HRMS: calcd for Ci8H17N2ON: 277.1341 ; found, 277.1392.
3-(3-Methylbenzyl)-5-phenylpyrazin-2-ol (H-l 1): This compound was obtained from E-7 and/or E-8 as a powder (5.52 g, 88 %) after a chromatography over silica gel (dichloromethane - ethanol 99/1 - 98/2) and a sample (0.29 g) was recrystallized in a mixture of toluene and cyclohexane (0.27 g). Ή NMR (DMSO-< 6) 12.38 (s, 1H), 7.87 (s, 1 H), 7.84 (m, 2H), 7.40 (m, 2H), 7.28 (m, 1H), 7.16 (m, 3H), 7.01 (m, 1H), 4.03 (s, 2H), 2.26 (s, 3H). HRMS: calcd for Ci8H17N2ON: 277.1341 ; found, 277.1395.
3-Benzyl-5-(2-methoxyphenyl)pyrazin-2-ol (H-l 2): This compound was obtained from E-9 and/or E-10 as a powder (0.54 g, 79 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3) Ή NMR (CDC13): 13.33 (s(br), 1H), 8.05 (s, 1 H), 8.02 (dd, J = 7.7, 1.7 Hz, 1 H), 7.51 7.42 (m, 2H), 7.37 7.28 (m, 3H), 7.25 - 7.19 (m, 1H), 7.08 (td, J = 7.7, 1.0 Hz, 1 H), 7.02 - 6.94 (m, 1 H), 4.27 (s, 2H), 3.92 (s, 3H). HRMS: calcd for C,8H|7N202, 293.1290; found, 293.1253.
3-Benzyl-5-(4-methoxyphenyl)pyrazin-2-ol (H-l 3): This compound was obtained from E-13 and/or E-14 as a powder (0.9 g, 45 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2). Ή NMR (DMSO-tf6) 12.30 (s, l H), 7.77 (m, 3H), 7.30 (m, 2H), 7.27 (m, 2H), 7.21 (m, IH), 6.96 (m, 2H), 4.06 (s, 2H), 3.77 (s, 3H). HRMS: calcd for C, 8H17N202: 293.1290; found, 293.1284.
3-Benzyl-5-(3-methoxyphenyl)pyrazin-2-ol (H-l 4): An analytical sample of this compound was obtained as a white powder after a recrystallization of the resulting residue in toluene, the rest was used directly in the next step. Ή NMR (DMSO-i/6) 12.41 (s, 1H), 7.90 (s, 1 H), 7.41 (m, 8H), 6.85 (m, 1H), 4.07 (s, 2H), 3.77 (s, 3H). HRMS: calcd for C18H17N202: 293.1290; found, 293.1279.
3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-ol (H-l 5): This compound was obtained from (E-21) and/or (E-22) as a powder (0.8 g, 62 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2). Ή NMR (CDC13) 12.31 (s, II), 7.78 (m, 2H), 7.40 (m, 4H), 737 (m, 9H), 7.19 (m, IH), 7.03 (m, 2H), 5.13 (s, 2H), 4.06 (s, 2H). HRMS: calcd for C24H22N2O2: 369.1603; found, 369.1603.
3-Benzyl-5-(4-(benzyloxy)-3-fluorophenyl)pyrazin-2-ol (H-16): This compound was obtained from E-23 and/or E-24 as a powder (2 g) after a chromatography over silica gel (dichloromethane - ethanol 985/15) and a sample was recrystallized in toluene for analytical purposes. Ή NMR (DMSO- ) 12.40 (s, IH), 7.87 (s, IH), 7.68 (m, IH), 7.60 (m, IH), 7.48- 7.12 (m, 1 1H), 5.20 (s, 2H), 4.06 (s, 2H). HRMS: calcd for C24H20FN2O2: 387.1509; found, 387.1514.
3-benzyl-5-(2-fluorophenyl)pyrazin-2-ol (H-17): This compound was obtained from E-15 and/or E-16 as a white powder (3.38 g, 62 %) after a chromatography over silica gel (dichloromethane - ethanol 975/25) and a recrystallization in toluene. Ή NMR (DMSO-i )
12.38 (s, IH), 7.88 (m, IH), 7.68 (s, IH), 7.28 (m, 8H), 4.06 (s, 2H). HRMS: calcd for C17Hi4F 20: 281.1090; found, 281.1050.
3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-ol (H-18): This compound was obtained from E-17 as a white powder (1.45 g, 83 %) after a chromatography over silica gel (dichloromethane - ethanol 99/1). 12.49 (s, IH), 7.57 (s, IH), 7.47 (m, IH), 7.27 (m, 4H), 7.19 (m, 3H), 4.01 (s, 2H). HRMS: calcd for C-H13F2N2O: 299.0996; found, 299.0994.
3-benzyl-5-(4-fluorophenyl)pyrazin-2-ol (H-19): This compound was obtained from E-18 and/or E-19 as a white powder (0.43 g, 72 %) after a chromatography over silica gel (cyclohexane ethyl acetate 1/1). !H NMR (DMSO-</6) 12.4 (s, IH), 7.87 (m, 3H), 7.22 (m, 7H), 4.06 (s, 2H). HRMS: calcd for C7H.4FN2O: 281.1090; found, 281.1030.
3-Benzyl-5-(3-fluorophenyl)pyrazin-2-ol (H-20): This compound was obtained from E-20 as a 90 % pure orange powder (1.69 g) after a chromatography over silica gel (dichloromethane - ethanol 98/2). !H NMR (DMSO-rf6) 12.50 (s, IH), 7.98 (s, IH), 7.69 (m, IH), 7.64 (m, IH), 7.42-7.1 (m, 6H), 7.08 (m, IH), 4.07 (s, 2H). LCMS (m/z) = 281.
Synthesis of pyrazin-2-ol II via step d through of 5-oxo-2,3»4,5-tetrahydropyrazine 1- oxides J, representative preparation of 3-benzy!-5-phenylpyrazin-2-oI (H-7). Step 1, preparation of 6-benzy!-5-oxo-2-phenyl-2,3,4,5-tetrahydropyrazine 1 -oxide (J-l): The 3- benzyl-5-phenylpiperazin-2-one (intermediates E-1 E2) (0.44 g, 1.65 mmol) was dissolved in acetic acid (5 mL) and a 36 % solution of peracetic acid in acetic acid (0.77 g, 3.63 mmol) was added. This was stirred overnight, diluted in ethyl acetate, washed with water, brine, dried over molecular sieve and concentrated to dryness. The residue was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 1/1 to 1/2) to yield the N-oxide (0.26 g, 56 %) as a white powder. Nota, COSY correlations firmly established the structures of this compound. Ή NMR (CDC13): 7.43 (m, 211), 7.35-7.20(m, 6H), 7.15 (m, 2H), 6.84 (s (br), 1H), 5.12 (t, 1H, J= 4.4), 4.13 (s, 2H), 4.00 (dd, 1H, J = 4.4, 13.3), 3.63 (dt, 1H, J= 4.4, 13.3). HEMS: calcd for Ci7HI7N202: 281.1190; found, 281.1234. Step 2: thermal rearrangement of the N-oxide. In a microwave-adapted vial, crude J-1 (0.41 g, 1.46 mmol) was dissolved in chlorobenzene (5 mL). This was sealed and heated in a microwave oven at 190 °C for two hours. The solvent were removed in vacuum and the residue purified by a chromatography over silica gel (cyclohexane - ethyl acetate 3/2) to give 3-benzyl-5- phenylpyrazin-2-ol (H-7) (0.20 g, 52 % from intermediates E-1/E2) with analytical data identical with the one described above.
3-Benzyl-6-methyl-5-phenylpyrazin-2-ol (H-21): Obtained from E-35, or the other diastereoisomers, via 6-benzyl-3-methyl-5-oxo-2-phenyl-2,3,4,5-tetrahydropyrazine 1 -oxide, as described above after a chromatography over silica gel (cyclohexane - ethyl acetate 2/1) and a recrystallization in cyclohexane to obtain an analytically pure sample. Ή NMR (CDC13) 13.33 (s, 1 H), 7.48 (m, 6H), 7.39 (m, 1 H), 7.28 (m, 2H), 7.20 (m, 1 H), 4.19 (s, 2H), 2.37 (s, 311). HRMS: calcd for Ci8Hi7N20: 277.1341 ; found, 277.1344.
Synthesis of 2-benzyl-5H-chromeno[3,4-b]pyrazin-3-ol (H-22): Under an inert atmosphere, in a tube fitted with a Teflon cap, (E-36) (0.1 g, 0.34 mmol) and N-iodosuccinimide (0.16 g, 0.69 mmol) were stirred in dry DMF (4 mL) at 60 °C for 90 minute and then 24 hours at 20 °C. The resulting solution was diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The resulting residue was purified by a chromatography over silica gel (cyclohexane - ethyl acetate 3/1) to give the expected compound as a powder (0.03 g, 30 %). Ή NMR (DMSO-rf6): 12.33 (s, 1H), 7.78 (dd, 1H, J =1.6 et 7.6), 7.37-7.25 (m, 4H), 7.23-7.16 (m, 2H), 7.05 (dt, 1H, J = 1.0, 8.5), 6.91 (dd, III, J = 0.8, 8.1), 5.13 (s, 2H), 4.07 (s, 2H). HRMS: calcd for C18H15N202: 291.1 133, found, 291.1066.
General preparation of α-arainoamides F from a-amino esters B. In a steel reactor, the considered a-amino ester (0.065 mol, either as a free base or as a hydrochloride) was dispersed in 7 N methanol ic ammonia (50 mL). This was heated at 70 °C for 16-19 hours, concentrated to dryness and the residue was purified as further described below.
2-Amino-3-phenylpropanamide (F-l ) Obtained from the hydrochloride of phenylalanine ethyl ester as a white solid (10.29 g, 78 %) after 19 hours of heating and a dispersion of the residue in boiling isopropanol (three crops). Ή NMR (DMSQ-c/6): 7.71 (s(br), 1 H), 7.29 (m, 6H), 6.47 (s(br), 3H), 3.74 (dd, 1H, J= 5.9, 7.6), 3.04 (dd, 1H, J= 5.9, 13.7), 2.87 (dd, IH, J= 7.6, 13.7), nota: signals shifts are dependant on the sample concentration as well as its water content.
2-Amino-3-(tetrahydrofuran-2-yl)propanamide (F-2): Obtained from B-16 as a solid which was not further purified. !H NMR (CDC13): mixture of isomer: 7.23 (s, IH), 5.63 (s, IH), 4.02 (m, 2H), 3.88 (m, IH), 3.74 (m, IH), 3.56 (m, IH), 2.07 (m, IH), 1.89 (m, 5H), 1.54 (m, IH). HRMS: calcd for C7H15N20: 159.1134; found, 159.1122.
2-Amino-3-(pyridin-3-yl)propanamide (F-3): Obtained from B-23 as an orange solid (3.46 g, 99%). Ή NMR (DMSO- ): 8.42 (m, IH), 8.40 (dd, IH, J = 1.9, 4.6), 7.62 (m, IH), 7.26 (m, 2H), 6.95 (s (br), IH), 4.02 (m, 2H), 3.35 (dd, IH, J = 5.3, 8.0), 2.90 (dd, IH, J = 5.2, 13.6), 2.64 (dd, IH, J = 8.0, 13.6), 1.75 (m, 2H). HRMS: calcd for C8Hi2N30: 166.0980; found, 166.0984.
General procedure for the synthesis pyrazin-2-ol H via a condensation between glyoxal and a-aminoamides F. These compounds were prepared by following the protocols previously described (J. Am. Ghent. Soc , 1949, 78; US 4046763, 1977).
5-Phenylpyrazin-2-ol (H-23): Obtained from phenyl glyoxal and glycine amide hydrochloride as a beige powder (1.69 g, 48 %). !H NMR (DMSO- ) 12.43 (s, IH), 8.12 (d, IH, J = 1.3), 8.06 (s, IH), 7.85 (m, 2H), 7.42 (m, 2H), 7.30 (m, IH). HRMS: calcd for C10H9N2O: 173.0715; found, 173.0654.
5-phenyl-3-((tetrahydrofuran-2-yl)methyl)pyrazin-2-ol (H-24): Obtained from phenyl glyoxal and F-2 as a pink solid (1.02 g, 45 %). Ή NMR (CDC13): 13.22 (s(br), IH), 7.81 (m, 2H),
7.63 (s (br), IH), 7.44 (m, 2H), 7.35 (m, IH), 4.60 (p, IH, J = 6.6), 3.99 (m, IH), 3.80 (m, IH), 3.30 (dd, IH, J = 7.0, 14.8), 3.05 (dd, I H, J = 6.1, 14.8), 2.17 (m, IH), 1.98 (m, 2H), 1.76 (m, IH). HRMS: calcd for C15Hi7N202, 257.1290; found, 257.1281.
5-phenyl-3-(pyridin-3-ylmethyl)pyrazin-2-ol (H-25): Obtained from phenyl glyoxal and F-3 as a white solid (0.57 g, 11 %) after a dispersion of the crude ethyl acetate extract in dichloromethane and a chromatography over silica gel (dichloromethane - ethanol 95/5 to 9/1). Ή NMR (DMSO- ): 12.47 (s(br), IH), 8.57 (d, IH, J= 1.7), 8.43 (dd, IH, /= 1.7, 4.8), 7.89 (s, IH), 7.92 (m, 2H), 7.74 (m, IH), 7.40-7.25 (m, 4H), 4.10 (s, 2H). HRMS: calcd for C16H14N30, 264.1137; found, 264.1122.
General procedure for the synthesis of chloropyrazines L via steps j. Under a calcium- protected atmosphere, the considered 2-hydroxypyrazine H (0.02 mol) was dispersed in phenylphosphonic dichloride (10 mL) and the suspension was heated at 100 °C for, unless stated otherwise, 12h. The resulting solution was diluted in ethyl acetate and poured onto an excess of crashed ice and stirred for 15 min. This was made basic with 22 % ammonia and extracted with ethyl acetate. The organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness and purified as described below.
2-Chloro-5-phenylpyrazine (L-l): Obtained from H-23 as a yellow solid (1.42 g, 82 %) after heating at 100 °C for 4 hours and a chromatography over silica gel (cyclohexane - dichloromethane 1/1). Ή NMR (CDC13): 8.80 (d, 1H, J= 1.5), 8.64 (d, 1H, J = 1.5), 8.00 (m, 2H), 7.51 (m, 3H). HRMS: calcd for C10H8C1N2, 191.0376; found, 191.0322.
2-Chloro-3-methyl-5-phenylpyrazine (L-2): Obtained from H-l as a yellow solid (1.18 g, 74 %) after heating only at 80 °C for 12 hours and a chromatography over silica gel (cyclohexane - dichloromethane 1/1). Ή NMR (CDC13): 8.63 (s, IH), 8.01 (m, 2H), 7.50 (m, 3H), 2.75 (s, 3H). HRMS: calcd for CuHioClN2, 205.0533; found, 205.0464.
2-Chloro-3-(2-fluorobenzyl)-5-phenylpyrazine (L-3): Obtained from H-2 as a white solid (3.01 g, 79 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 94/6). Ή NMR (CDCI3): Ή NMR (CDCI3, 400 MHz): 8.70 (s, 1H), 7.98-7.94 (m, 2H), 7.53-7.45 (m, 3H), 7.31-7.25 (m, 2H), 7.14-7.09 (m, 2H), 4.44 (s, 2H). HRMS: calcd for C17H13CIFN2, 299.0751 ; found, 299.0749.
2-Chloro-3-(2-chlorobenzyl)-5-phenylpyrazine (L-4): Obtained from H-3 as a white solid (1.58 g, 73 %) after a recrystallization in ethanol. Ή NMR (CDC13): 8.70 (s, 1 H), 7.93 (m, 2H), 7.46 (m, 4H), 7.22 (m, 3H), 4.52 (s, 2H). HRMS: calcd for C17H13CI2N2: 315.0456; found, 315.0466.
2-Chloro-3-(2-methoxybenzyl)-5-phenylpyrazine (L-5): Obtained from H-4 as a white solid (0.73 g, 63 %) after a recrystallization in ethanol. Ή NMR (CDC13): Ή NMR (CDCi3, 400 MHz): 8.66 (s, 1H), 7.96 (m, 2H), 7.48 (m, 3H), 7.27 (m, 1 H), 7.14 (m, 1H), 6.93 (m, 2H), 4.40 (s, 2H), 3.83 (s, 2H). HRMS: calcd for C18H,6C1N20, 31 1.0951 ; found, 31 1.0949.
2 -Chloro-3 -(3 - fl uorobenzyl)-5 -phenylpyrazine (L-6): Obtained from H-5 as an oil (3.19 g, 86 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 97/3). Ή NMR (CDCI3): 8.69 (s, IH), 8.03 (m, 2H), 7.52 (m, 3H), 7.29 (m, 1H), 7.18 (m, 1 H), 7.12 (m, 1H), 6.96 (m, I H), 4.39 (s, 2H). HRMS: calcd for C17H,3C1FN2, 299.0751 ; found, 299.0749.
2- Chloro-3-(4-fluorobenzyl)-5-phenylpyrazine (L-7): Obtained from H-6 as a white solid (0.25 g, 29 % from E-32) after a chromatography over silica gel (cyclohexane - ethyl acetate 97/3). Ή NMR (CDC13): 8.68 (s, IH), 8.02 (m, 2H), 7.52 (m, 3H), 7.36 (m, 2H), 7.02 (m, 2H), 4.36 (s, 2H). HRMS: calcd for C17H13CIFN2, 299.0751 ; found, 299.0746.
3- benzyl-2-chloro-5-phenylpyrazine (L-8): Obtained from H-7 as a yellowish solid (1.60 g, 82 %) after a chromatography over silica gel (cyclohexane-dichloromethane 3:2). Alematively, this compound was obtained in 36 % yield under the same reaction conditions but using the N-oxide J-l . Ή NMR (CDCI3): 8.68 (s, 1 II), 8.08 - 8.00 (m, 2H), 7.58 - 7.47 (m, 3H), 7.44 - 7.40 (m, 2H), 7.37 - 7.32 (m, 2H), 7.30 - 7.25 (m, 1H), 4.41 (s, 2H). HRMS: calcd for C17H14CIN2, 281.0846; found, 281.0730.
2- Benzyl-3-chloro-5-methyl-6-phenylp>Tazine (L-9): Obtained from H-21 as an oil (0.38 g, 20 % overall from 6-benzyl-3-methyl-5-oxo-2-phenyl-2,3,4,5-tetrahydropyrazinc 1-oxide) after a chromatography over silica gel (cyclohexane - ethylacetate 97:3). Ή NMR (CDCI3, 400 MHz): 7.62-7.59 (m, 2H), 7.53-7.45 (m, 3H), 7.40-7.37 (m, 2H), 7.33-7.22 (m, 4H), 4.36 (s, 2H), 2.61 (s, 3H). HRMS: calcd for C!gH16ClN2, 295.1002; found, 295.1003.
3- Benzyl-2-chloro-5-(p-tolyl)pyrazine (L-10): Obtained from H-8 as a yellow solid (1.83 g, 22 % from l-methyl-4-(2-nitrovinyl)benzene) after a chromatography over silica gel
(cyclohexane-dichloromethane 3/2). Ή NMR (CDCI3): 8.64 (s, 1H), 7.94 (m, 2H), 7.48
7.23 (m, 7H), 4.39 (s, 2H), 2.45 (s, 3H). HRMS: calcd for C18Hi6ClN2, 295.1002; found, 295.0999.
3-Benzyl-2-chloro-5-(m-tolyl)pyrazine (L-l l): This compound was obtained from H-9 as a white solid (3.02 g, 33 % from l-methyl-3-(2-nitrovinyl)benzene) after heating for 18 hours and a chromatography over silica gel (cyclohexane-ethyl acetate 965/35). !H NMR (CDCI3):
8.66 (s, 1H), 7.84 (m, I H), 7.82 (m, 2H), 7.43 - 7.24 (m, 6H), 4.40 (s, 2H), 2.48 (s, 3H). HRMS: calcd for C18H16C1N2, 295.1002; found, 295.1014.
2-Chloro-3-(2-methylbenzyl)-5-phenylpyrazine (L-12): This compound was obtained from H- 10 as a solid (5.12 g, 89 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3): 8.69 (s, I H), 7.98 (m, 2H), 7.50 (m, 3H), 7.20 (m, 4H), 4.39 (s, 2H), 2.46 (s, 3H). HRMS: calcd for Ci8H16QN2, 295.1002; found, 295.1009.
2- Chloro-3-(3-methylbenzyl)-5-phenylpyrazine (L-13): This compound was obtained from H- 11 as a solid (5.03 g, 90 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96/4). Ή NMR (CDCI3): 8.68 (s, IH), 8.05 (m, 2H), 7.51 (m, 3H), 7.22 (m, 3H), 7.08 (m, IH), 4.37 (s, 2H), 2.36 (s, 3H). HRMS: calcd for ClgH,6ClN2) 295.1002; found, 295.0997.
3- Benzyl-2-chloro-5-(2-methoxyphenyl)pyrazine (L-14): Obtained from H-12 as a yellow solid (1.82 g, 81 %) after heating for 18 hours and a chromatography over silica gel (cyclohexane-dichloromethane 3/2). Ή NMR (CDCI3): 8.86 (s, IH), 7.91 (dd, IH, J = 7.6, 1.8), 7.48 - 7.36 (m, 3H), 7.34 - 7.28 (ra, 2H), 7.26 - 7.18 (m, I H), 7.1 1 (td, 1H, J= 7.6, 0.8), 7.03 (d, IH, J = 8.3), 4.37 (s, 2H), 3.90 (s, 3H). HRMS: calcd for CigH,6ClN20, 311.0951; found, 311.0965.
3-Benzyl-2-chloro-5-(2-fluorophenyl)pyrazine (L-15): Obtained from H- 17 as a yellow solid (3.30 g, 67 %) after heating for 1 hours and a chromatography over silica gel (cyclohexane- dichloromethane 2/1). Ή NMR (CDC13): 8.78 (d, 1H, J = 2.2), 8.04 (dt, 1H, J = 7.6, 1.8), 7.30 (m, 8H), 4.40 (s, 2H). HRMS: calcd for Ci7H13ClFN2, 299.0751; found, 299.0818.
3-Benzyl-2-chloro-5-(3-fluorophenyl)pyrazine (L-16): Obtained as a powder from H-20 after a chromatography over silica gel (cyclohexane-ethyl acetate 98/2) (0.98 g, 8 % from 1 -fluoro- 3-(2-nitrovinyl)benzene). Ή NMR (CDC13): 8.66 (s, 1H), 7.78 (m, 2H), 7.48 (m, 1H), 7.41- 7.24 (m, 4H), 7.18 (m, 1H), 4.40 (s, 2H). HRMS: calcd for Ci7Hi3ClFN2, 299.0751 ; found, 299.0749.
3-benzyl-2-chloro-5-(2,6-difiuorophenyl)pyrazine (L-17) : Obtained from H-18 as a white solid (0.75 g, 56 %) after a chromatography over silica gel (cyclohexane-dichloromethane 3/1). !H NMR (CDC13): 8.44 (m, 1H), 7.43 (m, 3H), 7.27 (m, 2H), 7.25 (m, IH), 7.05 (m, 2H), 4.40 (s, 2H). HRMS: calcd for C17HuCjF2N2, 317.0657; found, 317.0648.
3-benzyl~2-chloro-5-(4-fluorophenyl)pyrazine (L-18): Obtained from H-19 as a white solid (0.84 g, 52 %) after heating for 14 hours and a chromatography over silica gel (cyclohexane- dichloromethane 3/1). Ή NMR (CDCI3): 8.63 (s, 1H), 8.02 (m, 2H), 7.39 (m, 2H), 7.34 (m, 2H), 7.28 (m, I H), 7.19 (m, 2H), 4.39 (s, 2H). HRMS: calcd for Ci7H,3QFN2i 299.0751 ; found, 299.0763.
3-Benzyl-2-chloro-5-(4-methoxyphenyI)pyrazine (L-19): Obtained from (H-13) as a yellow solid (0.33 g, 35 %) after a chromatography over silica gel (cyclohexane-dichloromethane 3/2). Ή NMR (CDCI3): 8.61 (s, IH), 7.97 (m, 2H), 7.40 (m, 2H), 7.31 (m, 2H), 7.27 (m, IH), 7.02 (m, 2H), 4.37 (s, 2H), 3.89 (s, 3H). HRMS: calcd for C!8Hi6ClN20, 31 1.0951 ; found, 31 1.0955.
3-Benzyl-2-chloro-5-(3-methox>phenyl)pyrazine (L-20): This compound was obtained from the crude H-14 as a white powder (0.4 g, 32 % from E-1 l/E-12), after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3): 8.66 (s, IH), 7.97 (m, 2H), 7.58 (m, 2H), 7.41 (m, 3H), 7.33 (m, 2H), 7.25 (m, IH), 7.03 (m, IH), 4.39 (s, 2H), 3.90 (s, 3H). HRMS: calcd for Ci8H16ClN20, 31 1.0951 ; found, 31 1.0962.
3-Benzyl-5-(4-(benzyloxy)phenyl)-2-chloropyrazine (L-21): Obtained from H-15 as a yellow solid (5.66 g, 62 %) after heating for 18 hours and a chromatography over silica gel (cyclohexane-dichloromethane 1/1). Ή NMR (CDCI3): 8.61 (s, I H), 8.0 (m, 2H), 7.47-7.20 (m, lOH), 7.1 1 (m, 2H), 5.17 (s, 2H), 4.38 (s, 2H). HRMS: calcd for C24H20ClN2O, 387.1264; found, 387.1266.
3-Benzyl-5-(4-(benzyloxy)-3-fluorophenyl)-2-chloropyrazine (L-22): Obtained from H-16 as a yellow solid ( 1.39 g, 29 % from the nitrovinyl) after a chromatography over silica gel (cyclohexane-dichloromethane 1/1). Ή NMR (CDCI3): 8.58 (s, IH), 7.84 (m, IH), 7.70 (m, 1H), 7.50-7.24 (m, 10H), 7.11 (m, 1H), 5.24 (s, 2H), 4.37 (s, 2H). HRMS: calcd for C24H19CIFN2O, 405.1170; found, 405.1154.
2-benzyl-3-chloro-5H-chromeno[4,3-b]pyrazine (L-23): Obtained from H-22 as a white powder (0.01 g, 10 %) after a chromatography over silica gel (cyclohexane-dichloromethane 3/2). !H NMR (CDCI3): 8.15 (dd, 1H, J = 2.0, 7.8), 7.41-731 (m, 5H), 7.28-7.24 (m, 1H),
7.13 (dt, 1H, J= 1.0, 8.1), 7.00 (dd, 1H, J= 1.0, 8.3), 5.31 (s, 2H), 4.37 (s, 2H). HRMS: calcd for C18Hi4ClN20, 309.0795; found, 309.0804.
2- chloro-5-phenyl-3-(pvridin-3-ylmethyl)pyrazine (L-24): obtained from H-25 as an oil (0.42 g, 83 %) after a chromatography over silica gel (dichloromethane - ethanol 98/2). Ή NMR (CDCI3): 8.70 (m, 1H), 8.69 (s, 1 H), 8.52 (dd, 1H, J = 1.6, 4.9), 7.99 (m, 2H), 7.71 (m, 1H), 7.50 (m, 3H), 7.26 (ddd, 1H, J = 0.8, 4.9, 8.0), 4.38 (s, 2H). HRMS: calcd for C16H13C1N35 282.0798; found, 282.0795.
General procedure for the synthesis of bromopyrazines L (X = Br) via steps i and 1. Step i: preparation of the trifluoromethanesulfonate K. At room temperature, the considered hydroxypyrazine (3 mmol) was dissolved in dry dichloromethane (20 mL, stabilized by amylene, dried over 4A sieve). Triethylamine (0.142 mL, 3.15 mmol) and tri flic anhydride (0.51 mL, 3.06 mmol) were then added. The resulting solution was stirred for 40 minutes, diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The resulting residue was purified as described below. Step 1: Exchange reaction with sodium bromide. In a tube featuring a Teflon-coated screw cap, one of these purified inflate esters (2 mmol) and dry sodium bromide (0.51 g, 5 mmol) were dispersed in dry dimethyl formamide (4 mL). Triflic acid (0.16 mL, 1.8 mmol) was added, the tube was closed and heated at 120 °C overnight. The resulting solution was diluted in diluted in ethyl acetate, washed with water, brine, dried over magnesium sulfate and concentrated to dryness. The resulting residue was then further purified as described below.
3- Benzyl-5-phenylpyrazin-2-yl trifluoromethanesulfonate (K-l): Obtained from H-7 as a white powder (0.34 g, 30 %) after a chromatography over silica gel (cyclohexane- dichloromethane 4/1 to 1/1) Ή NMR (CDCI3): 8.60 (s, 1H), 8.05 - 7.97 (m, 2H), 7.57 - 7.49 (m, 3H), 7.39 - 7.30 (m, 4H), 7.29 - 7.23 (m, 1H), 4.32 (s, 2H). HRMS: calcd for CisHwFaNaOsS, 395.0677; found, 395.0695.
3-Benzyl-2-bromo-5-phenylpyrazine (L-25): Obtained from K-l as white crystals (0.02 g, 30 %) after a chromatography over silica gel (cyclohexane-dichloromethane 3:2). !H NMR (CDCI3): 8.63 (s, 1H), 8.05 - 7.97 (m, 2H), 7.56 - 7.47 (m, 3H), 7.43 - 7.37 (m, 2H), 7.35 - 7.29 (m, 2H), 7.28 - 7.21 (m, 1H), 4.41 (s, 2H). HEMS: calcd for C17H,4BrN2, 325.0340; found, 325.0319.
5-phenyl-3-((tetrahydrofuran-2-yl)methyl)pyrazin-2-yl trifluoromethanesulfonate (K-2): Obtained from (H-24) as an oil (0.37 g, 43 %) after a chromatography over silica gel (cyclohexane-dichloromethane 1/3). Ή NMR (CDC13): 8.60 (s, 1H), 8.01 (m, 2H), 7.53 (m,
3H), 4.51 (m, 1H), 3.95 (m, 1H), 3.77 (m, 1H), 3.27 (dd, 1H, J = 7.8, 14.1), 3.10 (dd, 1H, J= 5,4, 14.1), 2.17 (m, 1H), 1.96 (m, 2H), 1.76 (m, 1H). HRMS: calcd for C16H,6F3N204S,
389.0783; found, 389.0808.
2-Bromo-5-phenyl-3-((tetrahydrofuran-2-yl)methyl)pyrazine (L-26): Obtained from K-2 as an oil (0.19 g, 62 %) after a chromatography over silica gel (cyclohexane-dichloromethane 1/4 to dichloromethane ethanol 99/1). Ή NMR (CDC13): 8.62 (s, 1 H), 8.02 (m, 2H), 7.50 (m, 3H), 4.57 (p, 1H, J= 6.7), 3.99 (m, 1H), 3.80 (m, 1H), 3.42 (dd, 1H, J = 6.7, 14.6), 3.14 (dd, 1H, J = 6.7, 14.6), 2.12 (m, 1H), 1.99 (m, 2H), 1.76 (m, I H). HRMS: calcd for C15Hi6BrN20, 319.0446; found, 319.0464.
2-benzyl-5H-chromeno[4,3-b]pyrazin-3-yl trifluoromethanesulfonate (K-3): Obtained from H-22, as an oil and a sample was purified by a chromatography over silica gel (cyclohexane- dichloromethane 2/1). ¾ NMR (CDCI3): 8.15 (dd, IH, J= 1.7, 7.8), 7.44-7.30 (m, 6H), 7.14 (dt, IH, J = 1.1, 8.0), 7.02 (dd, IH, J = 0.9, 8.3), 5.31 (s, 2H), 4.30 (s, 2H). HRMS: calcd for Ci8Hi3F3N204S, 423.0627; found, 423.0638.
2-benzyl-3-bromo-5H-chromeno[4,3-b]pyrazine (L-27): Obtained from K-3, as white solid (0.04 g, 24 %) after a chromatography over silica gel (cyclohexane-dichloromethane 2/1). Nota: an improved yield (0.075 g, 40 %) was achieved when using a 10 equivalent excess of sodium bromide under the same reaction conditions. Ή NMR (CDC13): 8.15 (dd, IH, J - 1.6, 7.8), 7.44-7.23 (m, 6H), 7.12 (dt, IH, J = 1.0, 8.1), 7.02 (dd, IH, J = 0.7, 8.1), 5.32 (s, 2H), 4.39 (s, 2H). HRMS: calcd for C! 8H,3BrN20, 353.0290; found, 353.0295.
General procedure for the synthesis N-aryl a-aminoesters N from halogenopyrazines L and a-aminoesters B via steps n. In a 20 mL scalable vial, the considered 2- halogenopyrazine L (0.001 mol), the considered amino ester B (0.001 1 mol, either as a free base or as a hydrochloride salt), cesium carbonate (0.0022 mol or 0.0032 mol, 0.69 g or 1.04 g) and a 1/1 mixture of palladium acetate and 2,2'-bis(diphenylphosphanyl)- 1 , - binaphthalene (ΒΓΝΑΡ) (0.05 mmol, 47 mg) were weighted. The air was replaced by argon and, under an inert atmosphere, dry acetonitrile (8 mL) was injected (dry dimethyl formamide can also be used). This was heated at 60 °C for 12 hours using either an oil bath along with very fast stirring to break up the clumps of caesium hydrogen carbonate forming, or an ultra sound bath featuring a temperature regulation. The resulting dark red or black suspension was dispersed in dichloromethane; this was filtered and concentrated to dryness prior further purification as described below.
Ethyl 2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-methylpentanoate (N-l): Obtained from L- 8 and IleOEt as an oil (0.25 g, 52 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). N.B.: A degree of racemization (about 30 %) was observed, either upon storage of IleOEt as a free base or upon the N-arylation reaction. !H NMR (diastereoisomeric mixture, 0.30 / 0.70 ratio, CDC13): 8.41 (s, IH), 7.95 (m, 2H), 7.47 (m, 2H), 7.38-7.32 (m, 5H), 7.29-7.26 (m, III), 4.84 (m, 0.7H), 4.75 (m, 0.3H), 4.65 (m, 111), 4.31 (m, 2H), 4.15 (m, 2H), 1.83 (m, I H), 1.35 (m, 1 H), 1.24 (m, 3H), 1.02 (m, 1 H), 0.85 (m, 3H), 0.75 (m, 3H). HRMS: calcd for C25H30N3O2, 404.2338; found, 404.2348.
Ethyl 2-((3-benzyl-5-phenylp>Tazin-2-yl)amino)-3-cycIopentylpropanoate (N-2): Obtained from L-8 and B-13 as an oil (0.26 g, 58 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). !H NMR (CDCI3): 8.42 (s, 1 H), 7.95 (m, 2H), 7.47 (m, 2H), 7.38-7.32 (m, 5H), 7.29-7.26 (m, 1 H), 4.78 (d, 1 H, J = 7.4), 4.60 (m, 2H), 4.30 (d, 1 H, J = 15.1), 4.21 (d, 1 H, J= 15.1), 4.16 (q, 2H, J = 7.2), 1.83 (m, IH), 1.70-1.39 (m, 711), 1.24 (t, 3H, J= 7.2), 1.01 (m, 2H). HRMS: calcd for C27H32N3O2, 430.2495; found, 430.2485.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-cyclohexylpropanoate (N-3): Obtained from L-8 and B-14 as an oil (0.36 g, 78 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). !H NMR (CDCI3): 8.41 (s, 1 H), 7.95 (m, 2H), 7.47 (m, 2H), 7.38-7.32 (m, 5H), 7.29-7.26 (m, 1 H), 4.60 (m, 4H), 4.30 (d, 1 H, J = 15.5), 4.20 (d, 1 H, J= 15.5), 4.16 (m, 2H), 1.60 (m, 6H), 1.48 (m, IH), 1.24 (t, 3H, J= 7.2), 1.08 (m, 4H), 0.86 (m, 2H). HRMS: calcd for C28H34N3O2, 444.2651 ; found, 444.2647.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(bicyclo[2.2.1 ]heptan-2-yl)propanoate (N- 4): Obtained from (L-8) and (B-15) as an oil (1.48 g, 82 %) as a mixture of 4 diastereoisomers (as seen by the number of CO signals in the 13C spectrum) after a chromatography over silica gel (cyclohexane ethyl acetate 95/5). !H NMR (CDCI3): 8.41 (s, I H), 7.95 (m, 2H), 7.46 (m,
211), 7.38-7.25 (m, 6H), 4.77 (m, IH), 4.56 (m, I II), 4.31 - 4.16 (m, 4H), 2.17 (m, 0.4H), 2.10 (m, 0.6H), 2.02 (m, 0.4H), 1.90 - 0.88 (m, 14H), 0.50 (m, 0.6H). HRMS: calcd for C29H34N3O2, 456.2651 ; found, 456.2661.
Ethyl (3-benzyl-5-phenylpyrazin-2-yl)phenylalaninate (N-5): Obtained from L-8 and PheOEt, HC1 as an oil (1.07 g, 69 %) after a chromatography over silica gel (dichloromethane). Ή NMR (CDCI3): 8.43 (s, IH), 7.96 (m, 2H), 7.53 - 7.43 (m, IH), 7.41 - 7.34 (m, I H), 7.33 - 7.20 (m, 8H), 7.05 - 6.95 (m, 2H), 4.97 (m, 2H), 4.14 (s, 2H), 4.13 (q, J = 7.1 Hz, 2H), 3.18 (dd, J = 13.8, 5.4 Hz, 1H), 3.09 (dd, J = 13.8, 5.9 Hz, 1H), 1.20 (t, J= 7.1 Hz, 3H). HEMS: calcd for C28H28N3O2, 438.2182; found, 438.2185.
Ethyl (3-benzyl-6-methyl-5-phenylpyrazin-2-yl)phenylalaninate (N-6): Obtained from L-9 and PheOEt, HCl as an oil (0.4 g, 84 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 94/6). !H NMR (CDCI3, 400 MHz): 7.60-7.57 (m, 2H), 7.48-7.44 (m, 2H), 7.39-7.35 (m, 1H), 7.31-7.20 (m, 8H), 7.03-7.00 (m, 2H), 5.00-4.95 (q, 1H, J = 7.0), 4.80- 4.78 (m, 1 H), 4.18-4.09 (m, 4H), 3.17-3.12 (m, 1 H), 3.08-3.03 (m, 1 H), 2.46 (s, 3H), 1.22- 1.18 (t, J = 7.9 Hz, 3H). HRMS: calcd for C29H30N3O2, 452.2338; found, 452.2347.
Ethyl 2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-phenylbutanoate (N-7): Obtained from L-8 and B-41 as an oil (0.3 g, 67 %) after a a chromatography over silica gel (cyclohexane - ethyl acetate 96/4). Ή NMR (diastereoisomeric mixture, 0.6 / 0.4 ratio) (CDCI3): 8.40 (s, 0.6H), 8.34 (s, 0.3H), 7.94 (m, 2H), 7.46 (m, 211), 7.39-7.19 (m, 7H), 7.21 (m, IH), 7.16 (m, 1H), 7.05 (m, 2H), 4.95 (m, 1H), 4.89 (d, 0.4H, J = 7.7), 4.69 (d, 0.6H, J = 8.3), 4.20 (m, 0.8H), 4.08 (m, 0.8H), 4.08 (m, 2.6H), 3.37 (m, 0.6H), 3.0 (m, 0.4H), 1.28 (d, 1.8H, J= 7.1), 1.24 (d, 0.8H, J = 7.3), 1.16 (t, 1.8H, J 7.5), 1.08 (t, 0.8H, J = 7.3). HRMS: calcd for C29H30N3O2, 452.2338; found, 452.2378.
Methyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(4- (benzyloxy)phenyl)propanoate (N-8): Obtained from L-21 PheOMe, HCl (0.53 g, 86 %) after a chromatography over silica gel (dichloromethane). Ή NMR (CDC13): 8.39 (s, I H), 7.98 - 7.87 (m, 2H), 7.53 - 7.47 (m, 4H), 7.47 - 7.41 (m, 4H), 7.41 - 7.35 (m, 2H), 7.34 - 7.27 (m, 3H), 7.25 - 7.21 (m, 2H), 7.15 7.07 (m, 2H), 6.94 - 6.82 (m, 4H), 5.17 (s, 2H), 5.09 (s, 2H), 5.03 - 4.94 (m, IH), 4.86 (d, IH, J = 7.4), 4.15 (s, 2H), 3.70 (s, 3H), 3.15 (dd, IH, J = 13.9, 5.3), 3.05 (dd, 1 H, J = 13.9, 6.0). HRMS: calcd for C41H38N3O4, 636.2863; found, 636.2897. Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(4- (benzyloxy)phenyl)propanoate (N-9): Obtained as an oil ( 1.17 g, 70 %) from L-21 and B-5 after a chromatography over silica gel (cyclohexane - ethyl acetate 91/9 to 9/1 ). Ή NMR (CDCI3): 8.34 (s, I H), 7.92 - 7.83 (m, 2H), 7.49 - 7.43 (m, 4H), 7.43 - 7.37 (m, 4H), 7.37 - 7.31 (m, 2H), 7.30 - 7.22 (m, 3H), 7.22 - 7.17 (m, 2H), 7.1 1 - 7.02 (m, 2H), 6.91 - 6.77 (m, 4H), 5.13 (s, 2H), 5.05 (s, 2H), 4.95 - 4.87 (m, IH), 4.86 - 4.79 (m, IH), 4.15 - 4.09 (m, 4H), 3.10 (dd, IH, J - 13.9, 5.4), 3.01 (dd, IH, J= 13.9, 5.9), 1.19 (t, 3H, J = 7.1). HRMS: calcd for C42H40N3O4, 650.3019; found, 650.3049.
Ethyl (3-benzyl-5-(4-(benzyloxy)-3-fluorophenyl)pyrazin-2-yl)phenylalaninate (N- 10): Obtained as an oil (0.66 g, 83 %) from L-22 and PheOEt, HCl after a chromatography over silica gel (cyclohexane - ethyl acetate 96/4 to 93/7). Ή NMR (CDC13): 8.33 (s, IH), 7.75 (m, IH), 7.60 (m, 1H), 7.49 (m, 2H), 7.41 (m, 2H), 7.37 - 7.18 (m, 9H), 7.07 (m, 1H), 7.98 (m, 2H), 5.21 (s, 2H), 4.12 (m, 4H), 3.16 (dd, 1H, J= 5.4, 14.1), 3.08 (dd, 1H, J= 5.7, 14.1), 1.19 (t, 3H, J - 7.1). HRMS: calcd for C35H33FN3O3, 562.2506; found, 562.2506.
Ethyl 2-((3-benzyl-5-phenylpyi-azin-2-yl)amino)-3-(tetrahydrofuran-2-yl)propanoate (N- 11): Obtained from L-8 and B-16 as an oil (0.50 g, 60 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1). !H NMR (diastereoisomeric mixture, 0.53 / 0.47 ratio) (CDCI3): 8.39 (s, 1H), 7.96 (rn, 2H), 7.45 (m, 2H), 7.35 (m, 5H), 7.25 (m, 1H), 4.78 (m, 0.6H), 4.59 (m, 0.4H), 4.20 (m, 4H), 3.81 (m, 1.6H), 3.70 (m, 1.4H), 2.1 (m, 1H), 1.88 (m, 4H), 1.46 (m, 1H), 1.25 (t, 1.4H, J = 7.2), 1.18 (t, 1.6H, J = 7.2). HRMS: calcd for C26H30N3O2, 432.2287; found, 432.2253.
Ethyl 2-(3-benzyl-5-phenylpyrazin-2-ylamino)-3-(l ,3-dioxolan-2-yl)propanoate (N- 12): Obtained from L-8 and B-43 as an oil (0.61 g, 67 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1 to 7/3). Ή NMR (CDC13): 8.41 (s, 1H), 7.94 (m, 2H), 7.46 (m, 2H), 7.37 - 7.31 (m, 5H), 7.25 (m, IH), 5.66 (m, IH), 4.85 (t, 2H, J= 4.3), 4.77 (m, 1 H), 4.23 (m, 2H), 4.18 (q, 2H, J= 7.1), 3.83 (m, 2H), 3.75 (m, 2H), 2.25 (m, 2H), 1.25 (t, 3H, J = 7.1). HRMS: calcd for
Figure imgf000086_0001
434.2080; found, 434.2117.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(2-methoxyphenyl)propanoate (N-13):
Obtained from L-8 and B-2 as a yellow solid (0.32 g, 77 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDCI3): 8.38 (s, IH), 7.90 (m, 2H), 7.44 (m, 2H), 7.34 (m, IH), 7.26 (m, 3H), 7.22 (m, 6H), 7.04 (dd, IH, J = 7.4, 1.7), 6.88 (td, 1 H, J = 7.4, 1.0), 6.83 (m, IH), 5.30 (d, 1H, J= 7.0), 4.85 (m, IH), 4.11 (m, 4H), 3.75 (s, 3H), 3.20 (dd, IH, J = 13.6, 5.6), 3.15 (dd, 1H, J = 13.6, 7.6), 1.20 (t, 3H, J = 7.1). HRMS: calcd for C29H30N3O3, 468.2287; found, 468.2281.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoate (N- 14): Obtained from L-8 and B-3 as an oil (0.30 g, 95 % pure) after a chromatography over silica gel (cyclohexane ethyl acetate 95/5). Ή NMR (CDCI3): 8.42 (s, IH), 7.95 (m, 2H), 7.46 (m,
2H), 7.36 (m, IH), 7.30 - 7.13 (m, 6H), 6.79 (m, 2H), 6.65 (m, IH), 6.58 (m, IH), 4.96 - 4.90 (m, 2H), 4.14 (m, 4H), 3.78 (s, 3H), 3.15 (dd, IH, J= 5.6, 13.8), 3.07 (dd, IH, J= 6.0, 13.8), 1.19 (t, 3H, J= 7.1). HRMS: calcd for C29H30N3O3, 468.2287; found, 468.2301.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-methoxyphenyl)propanoate ( -15): Obtained from L-8 and B-4 as an oil (0.16 g, 35 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 93/7). Ή NMR (CDCI3): 8.42 (s, IH), 7.95 (m, 2H), 7.46 (m, 2H), 7.39-7.21 (m, 6H), 6.89 (m, 2H), 6.76 (m, IH), 4.96 - 4.90 (m, 2H), 4.15 (rn, 4H), 3.81 (s, 3H), 3.12 (dd, IH, J= 4.8, 13.7), 3.03 (dd, IH, J= 5.2, 13.7), 1.21 (t, 3H, J = 7.1). HRMS: calcd for C29H30N3O3, 468.2287; found, 468.2271.
Ethyl 2-((3-benzyl-5-phcnylpyrazin-2-yl)amino)-3-(2-(trifluoromethyl)phenyl)propanoate (N-
16): Obtained from L-8 and B-24 as an oil (0.08 g, 18 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDCI3): 8.36 (s, IH), 7.93 (m, 2H), 7.62 (m, IH), 7.45 (m, 2H), 7.37 - 7.18 (m, 9H), 5.01 - 4.93 (m, 2H), 4.17 (s, 2H), 4.12 (m, 2H), 3.31 (m, IH), 3.15 (m, IH), 1.17 (m, 3H). HRMS: calcd for C29H27F3N3O2, 506.2055; found, 506.2053.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-(trifluoromethyl)phenyl)propanoate (N- 17) : Obtained from L-8 and B-25 as an oil (0.14 g, 31 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDCI3): 8.44 (s, IH), 7.99 (m, 2H), 7.62 (m, IH), 7.49 (m, 3H), 7.40 - 7.26 (m, 8H), 7.10 (m, IH), 5.02 (m, 2H), 4.17 (s, 2H), 4.13 (m, 2H), 3.25 (m, IH), 3.14 (m, IH), 1.19 (m, 3H). HRMS: calcd for C29H27F3N3O2, 506.2055; found, 506.2063.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-(trifluoromethyl)phenyl)propanoate (N-
18): Obtained from L-8 and B-26 as an oil (0.12 g, 26 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDC13): 8.44 (s, IH), 7.98 (m, 2H), 7.48 (m, 2H), 7.40 (m, 3H), 7.29 - 7.26 (m, 5H), 7.03 (m, 2H), 5.02 (m, 2H), 4.19 (m, 2H), 4.14 (s, 2H), 3.26 (dd, IH, J = 5.6, 13.6), 3.14 (dd, IH, J ~ 5.7, 13.6), 1.22 (t, 3H, J = 7.0). HRMS: calcd for C29H27F3N3O2, 506.2055; found, 506.2002.
Ethyl (3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl) phenylalaninate (N-19): Obtained from L-15 and PheOEt, HC1 as an oil (0.47 g, 81 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 91/9). Ή NMR (CDCI3): 8.53 (d, IH, J= 2.3), 8.04 (dt, 1H, J= 1.9, 7.9), 7.24 (m, 1 1H), 6.99 (m, 2H), 5.00 (m, 2H), 4.13 (m, 4H), 3.18 (dd, IH, J = 5.1, 13.7), 3.10 (dd, IH, J = 5.5, 13.7), 1.20 (t, 3H, J = 7.2). HRMS: calcd for C28H27EN3O2, 456.2087; found, 456.2079.
Ethyl 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(4-fluorophenyl)propanoate (N-
20): Obtained from L-15 and B-l as an oil (0.18 g, 90 % pure) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή MR (CDC13): 8.53 (d, 1 H, J = 2.3), 8.05 (dt, IH, J~ 1.9, 7.9), 7.37-7.14 (m, 8H), 6.88 (m, 2H), 6.86 (m, 2H), 4.99 (m, 2H), 4.15 (m, 4H), 3.16 (m, IH), 3.07 (m, IH), 1.20 (t, 3H, J= 7.1). HRMS: calcd for C28H26F2N3Q2, 474.1993; found, 474.1991.
Ethyl 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-21 ):
Obtained from L-15 and B-7 as an oil (0.42 g, 72 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 91/9). !H NMR (CDC13): 8.52 (d, 1H, J= 2.3), 8.03 (dt, 1H, J = 1.9, 7.9), 7.41-7.24 (m, 8H), 7.17 (ddd, IH, J = 1.3, 8.0, 11.4), 6.22 (dd, 1H, J = 1.8, 3.0),
5.84 (m, 1H), 5.19 (d(br), 1H, J= 7.6), 4.97 (m, IH), 4.17 (m, 4H), 3.21 (m, 2H), 1.22 (t, 3H, J= 7.1). HRMS: calcd for C26H25FN3O3, 446.1880; found, 446.1870.
Ethyl 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate
(N-22): Obtained from L-15 and B-8 as an oil (0.11 g, 30 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 98/2 to 95/5). Ή NMR (CDCI3): 8.52 (d, 1H, J= 2.3), 8.03 (dt, 1H, J= 1.9, 7.8), 7.35-7.24 (m, 7H), 7.16 (ddd, 1H, J= 1.2, 8.1, 11.1), 5.82 (m, 1 H), 5.75 (d, 1H, J = 3.0% 5.19 (d(br), 1H, J = 7.5), 4.95 (m, 1H), 4.18 (m, 4H), 3.16 (d, 2H, / = 5.4), 2.24 (s, 3H), 1.24 (t, 3H, J = 7.2). HRMS: calcd for C27H27FN3O3, 460.2036; found, 460.2039.
Ethyl 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoate (N-23): Obtained from L-15 and B-10 as an oil (0.2 g, 46 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDC13): 8.51 (d, I H, J= 2.3), 8.03 (dt, IH, J = 1.9, 7.8), 7.35-7.23 (m, 7H), 7.16 (ddd, 1 H, J = 1.3, 8.1 , 9.4), 5.82 (m, IH), 5.75 (d, III, J= 3.0), 5.19 (d(br), IH, J= 7.5), 4.95 (m, IH), 4.18 (m, 4H), 3.15 (d, 2H, J= 5.3), 2.58 (q, 2H, J = 7.6), 1.24 (t, 3H, J = 7.2), 1.21 (t, 3H, J = 7.6). HRMS: calcd for C28H29FN3O3, 474.2193; found, 474.2186.
Ethyl 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(4,5-dimethylfuran-2- yl)propanoate (N-24): Obtained from L-15 and B-9 as an oil (0.48 g, 86 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). !H NMR (CDCI3): 8.51 (d, IH, J = 2.4), 8.02 (dt, IH, J = 1.9, 7.8), 7.35-7.23 (m, 7H), 7.16 (ddd, 1H, J = 1.3, 8.1, 9.4), 5.65 (s, IH), 5.19 (d(br), 1H, J= 7.5), 4.92 (m, IH), 4.16 (m, 4H), 3.11 (d, 2H, J= 5.1), 2.13 (s, 3H), 1.88 (s, 3H), 1.25 (t, 3H, J = 7.2). HRMS: calcd for C28H29F 3O3, 474.2193; found, 474.2203.
Ethyl 2-((3-benzyl-5-(3-fluorophenyl)pyrazin-2-yl)amino)-3-(5-mcthylfuran-2-yl)propanoate
(N-25): Obtained from L-16 and B-8 as an oil (0.14 g, 90 % pure) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDCI3): 8.73 (s, IH), 7.70 (m, 2H), 7.45-7.23 (m, 6H), 7.02 (m, IH), 5.82 (m, IH), 5.74 (dfbr), 1H, J = 3.1), 5.18 (d(br), IH, J= 7.5), 4.92 (m, IH), 4.17 (m, 4H), 3.15 (d, 2H, J= 5.5), 2.22 (m, 3H), 1.24 (t, 3H, J= 7.2). HRMS: calcd for C27H27FN3O3, 460.2036; found, 460.2040.
Ethyl 2-((3-benzyl-5-(4-fluorophenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-26): Obtained from L-18 and B-8 as an oil (0.12 g, 21 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 96/4). !H NMR (CDCI3): 8.36 (s, IH), 7.91 (ra, 2H), 7.34-7.23 (m, 511), 7.15 (m, 2H), 5.82 (m, 1H), 5.75 (d, IH, J= 3.0), 5.13 (d(br), 1H, J = 7.6), 4.92 (m, 1H), 4.17 (m, 4H), 3.15 (d, 2H, J= 5.3), 2.22 (s, 3H), 1.23 (t, 3H, J= 7.2). HRMS: calcd for C27H27FN303, 460.2036; found, 460.2039.
Ethyl 2-((3-Benzyl-5-(4-methoxyphenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2- yl)propanoate (N-27): Obtained, using DMF as the reaction solvant, from L-19 and B-8 as an oil (0.16 g, 44 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 94/6). 1H NMR (CDCb): 8.35 (s, 1H), 7.90 (m, 2H), 7.33-7.23 (m, 5H), 7.01 (m, 2H), 5.82 (m, III), 5.75 (d, 1H, J = 2.6), 5.06 (d(br), 1H, J = 7.5), 4.91 (m, 1H), 4.21 (m, 4H), 3.87 (s, 3H), 3.14 (d, 2H, J = 5.5), 2.22 (s, 3H), 1.23 (t, 3H, J = 7.2). HRMS: calcd for C28H30N3O4, 472.2236; found, 472.2230.
2-((3-Benzy!-5-(3-methoxyphenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N- 28): Obtained from L-20 and B-8 as oil (0.07 g, 20 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 94/6). 1H NMR (CDCI3): 8.41 (s, 1 H), 7.51 (m, 2H), 7.40-7.23 (m, 6H), 6.91 (m, 1H), 5.82 (m, 1 H), 5.75 (d, 1 H, J= 2.9), 5.06 (d(br), 1H, J= 7.3), 4.93 (m, I II), 4.17 (m, 4H), 3.89 (s, 3H), 3.14 (d, 2H, J = 5.4), 2.23 (s, 311), 1.24 (t, 3H, J = 7.2). HRMS: calcd for C28H30N3O4, 472.2236; found, 472.2250.
Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2- yl)propanoate (N-29): Obtained, using DMF as the reaction solvant, from L-21 and B-8 as an oil (0.31 g, 50 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5). Ή NMR (CDCI3): 8.36 (s, 1 H), 7.89 (m, 2H), 7.50-7.23 (m, 10H), 7.01 (m, 2H), 5.81 (m, 1 H), 5.76 (d, 1 H, J= 2.6), 5.15 (s, 2H), 5.07 (d(br), 1H, J = 7.5), 4.91 (m, 1 H), 4.19 (m, 4H), 3.15 (d, 2H, J = 5.5), 2.24 (s, 3H), 1.23 (t, 3H, J = 7.2). HRMS: calcd for C34H34N3O4, 548.2549; found, 548.2533.
Ethyl (3-benzyl-5-(2,6-difiuorophenyl)pyrazin-2-yl)phenylalaninate (N-30): Obtained from L- 17) and PheOEt, HC1 as an oil (0.24 g, 65 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1 ). Ή NMR (CDCI3): 8.14 (s, 1 H), 7.30-7.19 (m, 9H), 7.04 - 6.96 (m, 4H), 5.01 (m, 2H), 4.12 (m, 2H), 4.1 1 (s, 2H), 3.16 (dd, 1 H, J = 5.2, 13.8), 3.08 (dd, 1H, J = 5.9, 13.8), 1.18 (t, 3H, J = 7.1). HRMS: calcd for C28H25F2N3O2, 474.1993; found, 474.201 1.
Ethyl 2-((3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N- 31): Obtained from L-17 and B-7 as an oil (0.19 g, 52 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 9/1). Ή NMR (CDCI3): 8.13 (s, 1H), 7.36-7.19 (m, 7H), 7.04 - 6.98 (m, 2H), 6.23 (m, 1 H), 5.84 (m, 1 H), 5.22 (d, 1H, J= 7.3), 4.96 (m, 1H), 4.16 (s, 2H), 4.15 (m, 2H), 3.20 (d, 2H5 J = 5.3), 1.12 (t, 3H, J = 7.0). HRMS: calcd for C26H23F2N3O3, 464.1786; found, 464.1799.
Isopropyl (5-phenylpyrazin-2-yl)phenylalaninate (N-32): Obtained from L-l and PheOiPr,
HCl as a solid, using dimethyl formamide as a solvent, after a chromatography over silica gel (cyclohexane - ethyl acetate 5/1). Ή NMR (CDCI3): 8.48 (m, 1 H), 8.01 (m, 1H), 7.89 (m, 2H,), 7.46 (m, 2H), 7.39-7.19 (m, 6H), 5.15 (d(br), 1H, J= 7.7), 5.07 (sept, 1 H, J = 6.3), 4.92 (m, IH), 3.29 (dd, 1H, J = 6.2, 13.8), 3.22 (dd, 1H, J = 6.0, 13.8), 1.27 (d, 3H, J = 6.3), 1.22 (d, 3H, J= 6.3). HRMS: calcd for C22H24N3O2, 362.1869; found, 362.1851.
Isopropyl (3-methyl-5-phenylpyrazin-2-yI)phenylalaninate (N-33): Obtained from L-2 and PheOiPr, HCl as a solid, using dimethyl formamide, after a chromatography over silica gel (cyclohexane - ethyl acetate 6/1). !H NMR (CDCI3): 8.36 (s, 1H), 7.90 (m, 2H), 7.46 (m, 2H), 7.37-7.18 (m, 6H), 5.06 (sept, 1H, J= 6.3), 5.01 (m, 1H), 4.88 (d(br), 1H, J = 7.3), 3.31 (dd, 1H, J = 6.2, 13.7), 3.22 (dd, IH, J = 6.0, 13.7), 1.27 (d, 3H, J = 6.3), 1.22 (d, 3H, J = 6.3). HRMS: calcd for C23H26N302, 376.2025; found, 376.2027.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-34): Obtained from L-8 and B-7 as an oil (6.31 g, 89 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). !H NMR (CDCI3): 8.42 (s, 1 H), 7.99 - 7.92 (m, 2H), 7.52 - 7.42 (m, 2H), 7.39 - 7.28 (m, 5H), 7.28 - 7.24 (m, 2H), 6.22 (dd, J = 3.2, 1.9 Hz, IH), 5.85 (dd, J = 3.2, 0.7 Hz, IH), 5.13 (d, J = 7.5 Hz, IH), 4.95 (dt, J= 7.5, 5.3 Hz, IH), 4.23 - 4.11 (m, 4H), 3.24 - 3.18 (m, 2H), 1.22 (t, J = 7.1 Hz, 3H). HRMS: calcd for C26H26N303j 428.1974; found: 428.1965.
Ethyl 2-(3-benzyl-5-phenylpyrazin-2-ylamino)-3-(furan-3-yl)propanoate (N-35): Obtained from L-8 and B-l l as an oil (1.2 g, 89 %) after a chromatography over silica gel ■ (cyclohexane-ethyl acetate 95/5 to 9/1). Ή NMR (CDCI3): 8.43 (s, IH), 7.97 (m, 2H), 7.48 (m, 2H), 7.40 - 7.24 (m, 7H), 6.96 (m, IH), 5.96 (m, IH), 5.00 (m, IH), 4.93 (m, IH), 4.24 -
4.14 (m, 4H), 3.02 (dd, IH, J = 5.1, 14.7), 2.95 (dd, IH, J = 5.4, 14.7), 1.22 (t, 3H, J = 7.2). HRMS: calcd for C^e aOs, 428.1974; found: 428.2006.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-36):
Obtained from L-8 and B-8 as an oil (0.69 g, 74 %) after a chromatography over silica gel (dichloromethane). !H NMR (CDCI3): 8.38 (s, IH), 7.96 - 7.89 (m, 2H), 7.48 - 7.40 (m, 2H), 7.38 - 7.26 (m, 5H), 7.25 - 7.19 (m, IH), 5.83 - 5.77 (m, IH), 5.72 (d, J= 3.0 Hz, IH), 5.11 (d, J = 7.5 Hz, IH), 4.89 (dt, J - 7.5, 5.3 Hz, IH), 4.21 - 4.08 (m, 4H), 3.12 (d, J = 5.3 Hz, 2H), 2.20 (s, 3H), 1.21 (t, J = 7.1 Hz, 3H). HRMS: calcd for C27H28N3O3, 442.2131; found, 442.2121. Ethyl 2-((3-benzyl-5-(p-tolyl)pyrazin-2-yl)arnino)-3-(5-methylfiiran-2-yl)propanoate (N-37):
Obtained from L-10 and B-8 as an oil (0.23 g, 45 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.38 (s, 1H), 7.83 (m, 2H), 7.28 (m, 2H),
7.26 - 7.17 (m, 7H), 5.81 (m, 1H), 5.74 (d, ΓΗ, J = 3.0), 5.07 (d, 1H, J = 7.9), 4.91 (m, 1H), 4.15 (m, 4H), 3.13 (d, 2H, J = 5.3), 2.41 (s, 3H), 2.22 (s, 3H), 1.24 (t, 3H, J = 7.2). HRMS: calcd for C28H30N3O3, 456.2287; found, 456.2274.
Ethyl 2-((3-benzyl-5-(m-tolyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-38):
Obtained from L-l l and B-8 as an oil (0.20 g, 26 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96:4). 1 H NMR (CDC13): 8.39 (s, 1H), 7.77 (m, 1H), 7.72 (m, 1H), 7.37 - 7.23 (m, 6H), 7.17 (m, 1H), 5.81 (m, 1H), 5.74 (d, IH, J = 3.0), 5.09 (d, 1H, J = 7.5), 4.91 (m, 1H), 4.17 (m, 4H), 3.12 (d, 2H, J = 5.3), 2.45 (s, 3H), 2.22 (s, 3H), 1.22 (t, 3H, J = 7.2). HRMS: calcd for C28H30N3O3, 456.2287; found, 456.2253.
Ethyl (3-benzyl-5-(2-methoxyphenyl)pyrazin-2-yl)phenylalaninate (N-39): Obtained from L- 14 and PheOEt, I1C1 as an oil (0.42 g, 72 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 90:10). Ή NMR (CDC13): 8.59 (s, 1 H), 7.87 (m, 1H), 7.37 - 7.26 (m, 2H), 7.26 - 7.17 (m, 711), 7.08 (m, 1H), 7.03 - 6.92 (m, 3H), 5.01 - 4.93 (m, 1H), 4.86 (d, J = 7.5 Hz, 1 H), 4.11 (s, 2H), 4.10 (q, J = 7.1 Hz, 2H), 3.89 (s, 3H), 3.15 (dd, J = 13.8, 5.5 Hz, IH), 3.06 (dd, J = 13.8, 6.0 Hz, 1H), 1.16 (t, J = 7.1 Hz, 3H). HRMS: calcd for C2vH3oN303, 468.2287; found, 468.2282.
Ethyl 2-((3-benzyl-5-(2-methoxyphenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-
40) : Obtained from L-14 and B-7 as an oil (0.40 g, 64 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 90:10). Ή NMR (CDC13): 8.58 (s, 1H), 7.87 (dd, J = 7.7, 1.8 Hz, IH), 7.37 - 7.27 (m, 5H), 7.25 - 7.19 (m, 2H), 7.08 (td, J= 7.5, 1.0 Hz, I H), 7.00 (dd, J= 8.3, 0.6 Hz, IH), 6.20 (dd, J= 3.1, 1.8 Hz, IH), 5.82 (dd, J= 3.1, .0.5 Hz, IH), 5.07 (d, J= 7.6 Hz, IH), 5.00 - 4.91 (m, IH), 4.15 (s, 2H), 4.13 (qd, J = 7.1, 1.4 Hz, 2H), 3.89 (s, 3H), 3.19 (d, J = 5.3 Hz, 2H), 1.19 (t, J - 7.1 Hz, 3H). HRMS: calcd for C27H28N304, 458.2080; found, 458.2083.
Ethyl 2-((3-benzyl-5-(2-methoxyphenyl)pyrazin-2-yl)amino)-3-(furan-3-yl)propanoate (N-
41) : Obtained from L-14 and B-11 as an oil (0.53 g, 84 %) after a ctiromatography over silica gel (cyclohexane-ethyl acetate 90:10). Ή 'NMR (CDC13): 8.60 (s, IH), 7.89 (dd, J = 7.6, 1.7
Hz, IH), 7.38 - 7.19 (m, 7H), 7.13 - 7.04 (m, IH), 7.00 (d, J= 8.3 Hz, IH), 6.94 (s, IH), 5.97 - 5.91 (m, IH), 5.02 - 4.87 (m, 2H), 4.24 - 4.07 (m, 4H), 3.89 (s, 3H), 2.97 (qd, J= 14.7, 4.8 Hz, 2H), 1.21 (t, J = 7.1 Hz, 3H). HRMS: calcd for C27H28N304, 458.2080; found, 458.2095. Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(pyridin-2-yl)propanoate (N-42): Obtained from L-8 and B-22 as an oil (0.45 g, 64 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5 to 75:25). ! H NMR (CDC13): 8.42 - 8.39 (m, 1H), 8.38 (s,
1H), 7.95 -· 7.89 (m, 2H), 7.52 (td, J= 7.6, 1.8 Hz, 1H), 7.47 - 7.39 (m, 2H), 7.36 - 7.29 (m, 3H), 7.29 - 7.23 (m, 2H), 7.23 - 7.18 (m, 1 H), 7.1 1 (ddd, J= 7.6, 4.9, 1.0 Hz, 1 H), 7.01 (d, J = 7.8 Hz, 1H), 6.30 (d, J= 7.3 Hz, 1 H), 5.03 (dt, J= 7.3, 5.4 Hz, 1H), 4.20 (s, 2H), 4.08 (qd, J = 7.1, 1.1 Hz, 2H), 3.39 - 3.27 (m, 2H), 1.11 (t, J = 7.1 Hz, 3H). FIRMS: calcd for C27H27N4Q2, 439.2134; found, 439.2152.
Ethyl (5 -phenyl-3 -((tetrahydrofuran-2 -yl)methyl)pyrazin-2-yl)phenylalaninate (N-42 ' ) : Obtained as a mixture of two diastereoisomers from L-26 and PheOEt, HC1 as an oil (0.06 g, 21 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 93:7). !H NMR (CDCI3): 8.34 (s, 0.5H), 8.33 (s, 0.5H), 7.89 - 7.84 (m, 2H), 7.45 -- 7.40 (m, 2H), 7.35 - 7.21 (m, 6H), 6.62 (d, J = 7.5 Hz, 0.5H), 6.44 (d, J = 7.1 Hz, 0.5H), 4.95 (dd, J = 13.0, 6.8 Hz, 0.5H), 4.86 (td, J = 7.4, 5.9 Hz, 0.5H), 4.37 - 4.30 (m, 0.5H), 4.30 - 4.23 (m, 0.5H), 4.22 - 4.13 (m, 2H), 3.89 - 3.79 (m, 0.5H), 3.77 - 3.58 (m, 1 .5H), 3.31 - 2.88 (m, 4.5H), 2.17 - 2.00
(m, 1H), 1.92 1.61 (m, 3.5H), 1.22 (t, J= 7.1 Hz, 1.5H), 1.21 (t, J = 7.1 Hz, 1.5H). HRMS: calcd for C26H3oN303, 432.2287; found, 432.2273.
Ethyl 2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-(4,5-dimethylfuran-2-yl)propanoate (N-
43): Obtained from L-8 and B-9 as an oil (0.20 g, 49 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.39 (s, 1H), 7.97 7.90 (m, 2H),
7.50 - 7.40 (m, 2H), 7.39 - 7.19 (m, 6H), 5.65 (s, 1H), 5.11 (d, J= 7.5 Hz, 1 H), 4.88 (dt, J = 7.5, 5.4 Hz, 1H), 4.19 - 4.12 (m, 4H), 3.09 (d, J= 5.4 Hz, 2H), 2.12 (s, 3H), 1.87 (s, 3H), 1.21 (t, J= 7.1 Hz, 3H). HRMS: calcd for C28H30N3O3, 456.2287; found, 456.2337.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4,5-dimethylthiophen-2-yl)propanoate (N-44): Obtained from L-8 and B-17 as an oil (0.24 g, 47 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96:4). !H NMR (CDCI3): 8.42 (s, 1H), 7.95 (m, 2H), 7.47 (m, 2H), 7.36 (m, 1H), 7.28 (m, 5H), 6.33 (s, 1H), 5.12 (d, J= 7.3 Hz, 1 H), 4.92 (dt, 1H, J = 7.2, 5.0), 4.18 (m, 4H), 3.32 (dd, 1H, /= 4.7, 14.8), 3.26 (dd, HI, J - 5.4, 14.8), 2.28 (s, 3H), 2.06 (s, 3H), 1.24 (t, J = 7.1 Hz, 3H). HRMS: calcd for C28H3QN302S, 472.2059; found, 472.2054.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4,5-dimethyloxazol-2-yl)propanoate (N-
45): Obtained from L-8 and B-40 as an oil (0.20 g, 49 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 97:3 to 3/1). lU NMR (CDCI3): 8.39 (s, 1H), 7.93 (m, 2H), 7.46 (m, 2H), 7.37 - 7.21 (m, 611), 5.75 (d, J= 7.8 Hz, 1 H), 5.07 (m, 1H), 4.20 (m, 2H), 4.15 (q, J = 7.1 Hz, 2H), 3.30 (dd, J = 5.6 .and 15.3 Hz, 1H), 3.22 (dd, J = 5.4 and 15.3 Hz, 1H), 2.17 (s, 3H), 2.05 (s, 3H), 1.18 (t, J = 7.1 Hz, 3H). HRMS: calcd for C27H29N403, 457.2240; found, 457.2234.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-fluorophenyl)propanoate (N-46): Obtained from (L-8) and (B-l) as an oil (0.45 g, 69 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). lH NMR (CDC13): 8.41 (s, 1H), 7.99 - 7.91 (m, 2H), 7.51 - 7.41 (m, 2H), 7.40 - 7.32 (m, 1 H), 7.31 - 7.25 (m, 311), 7.23 - 7.17 (m, 2H), 6.91 - 6.79 (m, 4H), 4.99 - 4.84 (m, 2H), 4.21 - 4.06 (m, 4H), 3.15 (dd, J = 13.9, 5.1 Hz, 1 H), 3.03 (dd, J = 13.9, 5.5 Hz, 1H), 1.20 (t, J = 1.1 Hz, 3H). HRMS: calcd for C28H27FN302, 456.2087; found, 456.21 10.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-fluorophenyl)propanoate (N-46'): Obtained from L-8 and B-32 as an oil (0.25 g, 64 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.44 (s, 1H), 7.97 (m, 2H), 7.48 (m, 2H), 7.38 (m, 1H), 7.32 -■ 7.23 (m, 5H), 7.17 (m, 111), 6.92 (m, 1H), 6.73 (m, 1H), 4.98 (m, 2H), 4.16 (m, 4H), 3.18 (dd. J - 5.5, 13.8 Hz, 1H), 3.03 (dd, J = 5.1, 13.8 Hz, III), 1.21 (t, J = 7.1 Hz, 3H). HRMS: calcd for C28H27FN 02, 456.2087; found, 456.2065.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(2-fluorophenyl)propanoate (N-47): Obtained from L-8 and B-27 as an oil (0.32 g, 79 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.42 (s, 1H), 7.96 (m, 2H), 7.47 (m, 2H), 7.37 (m, 1H), 7.27 (m, 6H), 6.98 (m, 3H), 4.99 (m, 2H), 4.15 (m, 4H), 3.22 (dd, 1 H, J = 13.9, 5.5), 3.16 (dd, 1H, J = 13.9, 6.3), 1.20 (t, 3H, J = 7.1). HRMS: calcd for C28H27FN302, 456.2087; found, 456.2084.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(2,4-difluorophenyl)propanoate (N-48): Obtained from L-8 and B-28 as an oil (0.27 g, 61 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96:4). Ή NMR (CDC13): 8.42 (s, 1 H), 7.96 (m, 2H), 7.47 (m, 2H), 7.37 (m, 1 H), 7.27 (m, 5H), 6.98 (m, 1H), 6.70 (m, 2H), 4.97 (m, 2H), 4.16 (m, 4H), 3.22 (dd, 1H, J = 5.4, 14.3), 3.10 (dd, 1H, J = 6.1 , 14.3), 1.22 (t, 3H, J = 7.1). HRMS: calcd for C28H26F2N302, 474.1993; found, 474.1988.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-chlorophenyl)propanoate (N-49): Obtained from L-8 and B-29 as an oil (0.38 g, 64 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 97:3 to 96:4). Ή NMR (CDC13): 8.43 (s, 1 H), 7.97 (m, 2H), 7.48 (m, 2H), 7.37 (m, IH), 7.30 (m, 3H) 7.22 (m, 2H), 7.14 (m, 2H) 6.85 (m, 2H), 4.95 (m, 2H), 4.15 (m, 4H), 3.16 (m, 1H), 3.03 (m, 1H), 1.23 (t3H, J = 7.2 Hz,). HRMS: calcd for C28H27CIN302, 472.1792; found, 472.1782. Ethyl 2-((3-benzyl-5-phenylp\Tazin-2-yl)amino)-3-(4-bromophenyl)propanoate (N-50):
Obtained from L-8 and B-31 as an oil (0.37 g, 57 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 97:3 to 96:4). !H NMR (CDC13): 8.43 (s, 1H), 7.97 (m, 2H), 7,48 (m, 2H), 7.37 (m, 1H), 7.29 (m, 5H), 7.22 (m, 2H), 6.79 (m, 2H), 4.95 (m, 2H), 4.15 (m, 4H), 3.16 (m, 1H), 3.03 (m, 1H), 1.23 (t, 3H, J= 7.2). HRMS: calcd for C^sI^BrN^, 516.1287; found, 516.1267.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(o-tolyl)propanoate (N-51): Obtained from L-8 and B-20 as an oil (0.48 g, 90 %) after a chromatography over silica gel (cyclohexane- ethyl acetate 95:5). Ή NMR (CDC13): 8.40 (s, 1 H), 7.94 (m, 2H), 7.48 - 7.42 (m, 2H), 7.38 - 7.24 (m, 6H), 7.15 (m, 2H), 7.06 (m, 1H), 6.93 (m, 1H), 4.92 (m, 2H), 4.16 (m,4H), 3.14 (dd, 1H, J = 6.1, 14.0), 3.02 (dd, 1H, J = 7.1, 14.0), 2.29 (s, 3H), 1.16 (t, 311, J - 7.1). HRMS: calcd for C29H29 3O2, 452.2338; found, 452.2352.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoate (N-52): Obtained from L-8 and B-6 as an oil (0.28 g, 61 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.41 (s, 1H), 8.07 - 7.90 (m, 2H), 7.48 ~ 7.42 (m, 2H), 7.38 - 7.32 (m, 1H), 7.30 - 7.26 (m, 1H), 7.26 - 7.23 (m, 1H), 7.21 - 7.16 (m, 2H), 7.15 - 7.08 (m, I H), 7.07 - 7.01 (m, 1H), 6.88 (s, 1H), 6.78 (d, 1H, J= 7.5), 4.93 (t, 1H, J = 5.9), 4.92 (t, IH, J = 6.5), 4.88 - 4.79 (m, 211), 4.11 (q, 2H, J = 7.1), 4.11 (q, 2H, J = 15.4), 3.11 (dd, 1H, J = 13.8, 5.5), 3.03 (dd, 1H, J = 13.8, 6.1), 2.30 (s, 3H), 1.17 (t, 3H, J = 7.1). HRMS: calcd for C29H29N3O2, 452.2338; found, 452.2408.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)araino)-3-(p-tolyl)propanoate (N-53): Obtained from L-8 and B-21 as an oil (0.35 g, 87 %) after a chromatography over silica gel (cyclohexane- ethyl acetate 94:6). Ή NMR (CDCI3): 8.43 (s, I H), 7.97 (m, 2H), 7.48 (m, 2H), 7.37 (m, III), 7.28 (m, 311), 7.22 (m, 2H), 7.04 (m, 2H), 6.89 (m, 2H), 4.93 (m, 2H), 4.15 (m, 4H), 3.15 (dd, IH, J = 13.8, 5.2), 3.06 (dd, IH, J = 13.8, 5.8), 2.35 (s, 3H), 1.21 (t, 3H, J = 7.2). HRMS: calcd for C29H29N3O2, 452.2338; found, 452.2325.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-propylphenyl)propanoate (N-54):
Obtained from L-8 and B-37 as an oil (0.27 g, 79 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). !H NMR (CDCI3): 8.42 (s, IH), 7.95 (m, 2H), 7.47 (m, 2H), 7.37 (m, IH), 7.26 (m, 5H), 7.16 (m, IH), 7.07 (m, IH), 6.92 (m, IH), 6.82 (m, IH), 4.93, (m, 2H), 4.14 (m, 4H), 3.15 (dd, IH, J = 13.7, 5.6), 3.07 (dd, IH, J = 13.7, 6.1), 2.56 (m, 2H), 1.63 (m, 2H), 1.18 (t, 3H, J = 7.2), 0.95 (t, 3H, J = 7.3). HRMS: calcd for C31H34N3O2, 480.2651; found, 480.2661. Ethyl 2-((3-benzyl-5-phcnylpyrazin-2-yl)amino)-3-(4-propylphenyl)propanoate (N-55):
Obtained from L-8 and B-36 as an oil (0.25 g, 73 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). !H NMR (CDC13): 8.42 (s, IH), 7.96 (m, 2H), 7.47 (m, 2H), 7.37 (m, 1H), 7.26 (m, 5H), 7.05 (m, 2H), 6.91 (m, 2H), 4.93, (m, 2H), 4.13 (m, 4H), 3.14 (dd, 1 H, J= 13.9, 5.5), 3.06 (dd, 1H, J = 13.9, 6.0), 2.59 (m, 2H), 1.65 (m, 2H), 1.19 (t, 3H, J = 7.2), 0.97 (t, 3H, J= 7.3). HRMS: calcd for C3iH34N302, 480.2651 ; found, 480.2671.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-isopropylphenyl)propanoate (N-56): Obtained from L-8 and B-33 as an oil (0.25 g, 73 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.40 (s, 1 H), 7.97 - 7.90 (m, 2H), 7.49 - 7.40 (m, 2H), 7.38 - 7.32 (m, 1H), 7.32 - 7.23 (m, 3H), 7.23 - 7.17 (m, 2H), 7.12 - 7.05 (m, 211), 6.94 - 6.88 (m, 2H), 4.99 - 4.84 (m, 2H), 4.18 - 4.06 (m, 4H), 3.08 (qd, 2H, J = 13.8, 5.7), 2.96 - 2.84 (m, 1H), 1.25 (d, 6H, J = 6.9), 1.16 (t, 3H, J = 7.1). HRMS: calcd for C3iH34N302, 480.2683; found, 480.2690.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-cyclopropylphenyl)propanoate (N-57): Obtained from L-8 and B-34 as an oil (0.46 g, 76 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.42 (s, 1 H), 7.96 (m, 2H), 7.47 (m, 2H), 7.36 (m, 1 H), 7.31-7.23 (m, 3H), 7.20 (m, 2H), 6.94 (m, 2H), 6.86 (m, 2H), 4.93, (m, 2H), 4.13 (m, 4H), 3.13 (dd, 1 H, J = 5.3, 13.9), 3.04 (dd, 1 H, J = 5.9, 13.9), 1.89 (m, 1H), 1.19 (t, 3H, J - 7.2), 0.98 (m, 2H), 0.69 (m, 2H). HRMS: calcd for C3,H32N302, 478.2495; found, 478.2487.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(3-cyclopropylphenyl)propanoate (N-58): Obtained from L-8 and B-35 as an oil (0.46 g, 79 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.42 (s, 1H), 7.95 (m, 2H), 7.47 (m, 2H), 7.36 (m, 1H), 7.28-7.19 (m, 5H), 7.13 (m, 1H), 6.95 (m, 1H), 6.82 (m, 1H), 6.77 (m, 1 H), 4.93, (m, 2H), 4.13 (m, 4H), 3.13 (dd, 1H, J - 5.5, 13.7), 3.05 (dd, J= 6.0, 13.7 Hz, 1H), 1.86 (m, 1 H), 1.18 (t, 3H, J = 7.2), 0.96 (m, 2H), 0.67 (m, 2H). HRMS: calcd for C3,H32N302, 478.2495; found, 478.2480.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(thiophen-2-yl)propanoate (N-59): Obtained from L-8 and B-12 as an oil (0.51 g, 80 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.42 (s, 1H), 8.00 - 7.89 (m, 2H), 7.50 - 7.42 (m, 2H), 7.40 - 7.32 (m, 1H), 7.32 - 7.26 (m, 3H), 7.26 - 7.21 (m, 211), 7.14 - 7.08 (m, 1 H), 6.85 (dd, J= 5.2, 3.4 Hz, 1H), 6.58 - 6.50 (m, IH), 5.12 (d, J= 7.1 Hz, 1H), 4.99 (dt, J = 7.1 , 5.0 Hz, IH), 4.17 (s, 2H), 4.14 (q, J = 7.1 Hz, 2H), 3.44 (dd, J - 14.6, 4.7 Hz, I H), 3.37 (dd, J- 14.9, 4.9 Hz, 1H), 1.22 (t, J= 7.1 Hz, 3H). HRMS: calcd for CieH^NaOaS, 444.1746; found, 444.1766.
Ethyl 2-((3 -benzyl-5-phenylpyrazin-2-yl)amino)-3-(3 -methylthiophen-2-yl)propanoate (N-
60): Obtained from L-8 and B-18 as an oil (0.41 g, 76 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.44 (s, 1H), 7.97 (m, 2H), 7.47 (m, 2H), 7.37 (m, 1H), 7.33 - 7.23 (m, 5H), 7.05 (d, 1H, J = 4.8), 6.75 (d, 1H, J = 4.8), 5.15 (d, 1H, J = 7.1), 4.96 (m, 1H), 4.15 (m, 4H), 3.41 (dd, 1 H, J = 5.1, 15.1), 3.31 (dd, 1 H, J = 5.1, 15.1), 2.0 (s, 3H), 1.23 (t, J = 7.3 Hz, 3H). HRMS: calcd for C27H28N3O2S, 458.1902; found, 458.1910.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethylthiophen-2-yl)propanoate (Ν-6Γ):
Obtained from L-8 and B-19 as an oil (0.47 g, 84 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.43 (s, 1H), 7.97 (m, 2H), 7.47 (m, 2H), 7.37 (m, 1H), 7.33 - 7.23 (m, 5H), 6.55 (m, 1H), 6.39 (m, 1H), 5.13 (d, 1H, J= 7.1), 4.91 (m, 1H), 4.17 (m, 4H), 3.34 (m, 2H), 2.79 (qd, 211, J= 0.8, 7.5), 1.30 (t, J = 7.5 Hz, 3H), 1.24 (t, J = 7.3 Hz, 3H). HRMS: calcd for C28H30N3O2S, 472.2059; found, 472.2055.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoate (N-62): Obtained from L-8 and B-10 as a yellow solid (0.48 g, 74 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). !H NMR (CDCI3): 8.39 (s, 1 H), 7.96 - 7.89 (m, 2H), 7.50 - 7.40 (m, 2H), 7.37 - 7.26 (m, 4H), 7.26 - 7.20 (m, 2H), 5.80 (dd, J= 2.0, 1.0 Hz, 1H), 5.74 (d, J= 3.0 Hz, 1 H), 5.09 (d, J = 7.5 Hz, 1H), 4.91 (dt, J = 7.5, 5.4 Hz, 1H), 4.22 - 4.08 (m, 4H), 3.14 (d, J= 5.3 Hz, 2H), 2.56 (qd, J= 7.5, 0.6 Hz, 2H), 1.21 (t, J= 7.3 Hz, 3H), 1.19 (t, J = 7.3 Hz, 3H). HRMS: calcd for C28H3oN303, 457.2319; found, 457.2314.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-cyclopropylfuran-2-yl)propanoate (N- 63): Obtained from L-8 and B-39 as an oil (0.31 g, 67 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3): 8.39 (s, 1H), 7.97 - 7.90 (m, 2H), 7.48 - 7.41 (rn, 2H), 7.38 - 7.20 (m, 6H), 5.78 (dd, J = 3.1, 0.5 Hz, 1H), 5.72 (d, J - 3.1 Hz, 1H), 5.08 (d, 1H, J = 7.6), 4.90 (dt, 1H, J = 7.6, 5.3), 4.20 - 4.11 (m, 4H), 3.12 (d, 2H, J = 5.3), 1.81 (tt, 1H, .7= 8.5, 5.1), 1.22 (t, 3H, J = 7.1), 0.88 - 0.80 (m, 2H), 0.73 - 0.65 (m, 2H). HRMS: calcd for C29H30N3O3, 468.2287; found, 468.2291.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-(trifluoromethyl)furan-2-yl)propanoate (N-64): Obtamed from L-8 and B-38 as an oil (0.17 g, 44 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 93/7). !H NMR (CDCI3): 8.40 (s, 1H), 7.99 - 7.92 (m, 2H), 7.50 - 7.42 (m, 2H), 7.40 - 7.33 (m, 1H), 7.33 - 7.20 (m, 5H), 6.55 (dd, J= 3.3, 1.2 Hz, 1H), 5.74 (dd, J = 3.3, 0.6 Hz, 1H), 5.12 (d, J = 7.1 Hz, 1H), 4.98 (dt, J = 7.1, 5.2 Hz, 1H), 4.26 - 4.09 (m, 4H), 3.29 (dd, J= 15.2, 5.2 Hz, 1H), 3.19 (dd, J= 15.2, 5.2 Hz, 1H), 1.24 (t, J
= 7.1 Hz, 3H). HRMS: calcd for C27H25F3N303, 496.1848; found, 496.1847.
Ethyl 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-(benzyloxy)phenyl)propanoate (N-65):
Obtained from L-8 and B-5 as an oil (0.68 g, 70 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDC13): 8.41 (s, 1H), 8.00 - 7.86 (m, 2H), 7.50 - 7.16 (m, 13H), 6.90 - 6.84 (m, 2H), 6.84 - 6.79 (m, 2H), 5.05 (s, 2H), 4.99 - 4.84 (m, 2H), 4.12 (qd, 2H, J = 7.1, 0.6), 4.12 (s, 211), 3.11 (dd, 1H, J = 13.9, 5.0), 3.01 (dd, 1 H, J = 13.9, 5.5), 1.19 (t, 3H, J= 7.1 ). HRMS: calcd for C35H33N303, 544.2600; found, 544.2654.
Ethyl (3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)phenylalaninate (N-66): Obtained, from L-21 and PheOEt, HC1 as white solid (4.47 g, 87 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 95/5 to 94/6). Ή NMR (CDC13): 8.36 (s, 1H), 7.89 (m, 2H), 7.49 (m, 2H), 7.42 (m, 2H), 7.35 (m, 1H), 7.32-7.20 (m, 811), 7.08 (m, 2H), 7.00 (m, 2H), 5.15 (s, 2H), 4.97 (m, IH), 4.86 (d(br), 1H, J= 8.1), 4.14 (m, 4H), 3.17 (dd, 1H, J = 5.5, 13.8), 3.08 (dd, 1 H, J = 6.0, 13.8), 1.19 (t, 3H, J= 7.2). HRMS: calcd for C35H34N303, 544.2600; found, 544.2609.
Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N- 67): Obtained, from L-21 and B-7 as an oil (0.83 g, 79 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 94/6 to 93/7). Ή NMR (CDC13): 8.35 (s, 1 H), 7.89 (m, 2H), 7.48 (m, 2H), 7.42 (m, 2H), 7.36 (m, IH), 7.32-7.20 (m, 5H), 7.08 (m, 2H), 6.22 (dd, 1H, J = 2.0, 3.0), 5.85 (dd, IH, J = 0.7, 3.0), 5.15 (s, 2H), 5.07 (d(br), 1H, J= 7.6), 4.94 (m, IH), 4.16 (m, 4H), 3.20 (d, 2H, J= 5.3), 1.21 (t, 3H, J= 7.2). HRMS: calcd for C33H32N304, 534.2393; found, 534.2410.
Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(5-ethylfuran-2- yl)propanoate (N-68): Obtained, from L-21 and B-10 as an oil (0.65 g, 60 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 94/6). Ή NMR (CDC13): 8.34 (s, I H), 7.88 (m, 2H), 7.48 (m, 2H), 7.42 (m, 2H), 7.36 (m, IH), 7.32-7.20 (m, 5H), 7.08 (m, 2H), 5.82 (m, IH), 5.76 (d, 1H, J - 3), 5.14 (s, 2H), 5.06 (d(br), 1 H, J = 7.5), 4.91 (m, I H), 4.16 (m, 4H), 3.15 (d, 2H, J = 5.4), 2.57 (q, 2H, J - 7.8), 1.23 (t, 3H, J= 7.2), 1.21 (t, 3H, J = 7.8). HRMS: calcd for C35H36N30 , 562.2706; found, 562.2716.
Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(4,5-dimethylfuran-2- yl)propanoate (N-69): Obtained, from L-21 and B-9 as an oil (0.62 g, 56 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 94/6). Ή NMR (CDC13): 8.34 (s, IH), 7.88 (m, 2H), 7.48 (m, 2H), 7.42 (m, 2H), 7.36 (m, IH), 7.32-7.20 (m, 5H), 7.07 (m, 2H), 5.66 (s, I H), 5.14 (s, 2H), 5.06 (d(br), IH, J = 7.4), 4.88 (m, IH), 4.17 (m, 4H), 3.10 (d, 2H, J = 5.4), 2.14 (s, 3H), 1.89 (s, 3H), 1.23 (t, 3H, J = 7.8). HRMS: calcd for C35H36N3O4, 562.2706; found, 562.2690.
Ethyl 2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(3- propyIphenyl)propanoate (N-70): Obtained, from L-21 and B-37 (0.97 g, 85 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 98/2). Ή NMR (CDCI3): 8.36 (s, 1 H), 7.89 (m, 2H), 7.32 (m, 1 1H), 7.07 (m, 3H), 6.92 (m, 1H), 6.82 (m, 1H), 5.15 (s, 211),
4.95 (m, 1H), 4.84 (d, 1H, J= 7.5), 4.12 (m, 4H), 3.14 (dd, 1H, J= 13.7, 5.7), 3.07 (dd, IH, J = 13.7, 6.2), 2.56 (m, 2H), 1.64 (m, 2H), 1.18 (t, 3H, J = 7.1), 0.96 (t, 3H, J = 7.3). HRMS: calcd for C38H40N3O3: 586.3069; found, 586.3096.
Ethyl 2-(3-benzyl-5-phenylp Tazin-2-ylamino)-4-phenylbutanoate (N-71 ): Obtained from L-8 and B-42 as an oil (0.36 g, 80 %) after a chromatography over silica gel (cyclohexane / ethyl acetate 95:5). lU NMR (CDCI3): 8.42 (s, 1H), 7.97 (m, 2H), 7.48 (m, 1 H), 7.38 (m, 5H), 7.28 (m, 3H), 7.20 (m, 1H), 7.06 (m, 2H), 4.97 (d (br), 1H, J= 7.0), 4.73 (m, 1 H), 4.27 (d, 1H, J=
15.4) , 4.21 (d, 1 H, J 15.4), 4.16 (q, 2H, J= 7.1), 2.43 (m, 2H), 2.21 (m, 1 H), 1.99 (m, IB), 1.26 (t, 3H, J= 7.1). HRMS: calcd for ¾Η30Ν3Ο2, 452.2338; found, 452.2335.
Ethyl (2-benzyl-5H-chromeno[3,4-b]pyrazin-3-yl)phenylalaninate (N-72): Obtained from L- 27 and PheOEt, HC1 as an orange oil (0.05 g, 33 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 93/7). !H NMR (CDCI3): 8.07 (dd, 1H, J - 1.6, 7.7), 7.38-7.18 (m, 9H), 7.12 (dt, I H, J= 1.0, 7.8), 7.01-6.93 (m, 3H), 5.18 (s, 2H), 5.01-4.91 (m, 2H), 4.20-4.06 (m, 4H), 3.15 (dd, 1H, J = 5.2, 13.9), 3.05 (dd, 1H, J = 5.9, 13.9), 1.22 (t, 3H, J = 7.8). HRMS: calcd for C29H2SN3O3, 466.2131; found, 466.2117.
Ethyl (5-phenyl-3-(pyridin-3-ylmethyl)pyrazin-2-yl)phenylalaninate (N-73): Obtained from L-24 and PheOEt, HC1 as an oil (0.28 g, 62 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 2/1). Ή NMR (CDCI3): 8.52 (m, 3H), 8.44 (s, 1H), 7.90 (m, 2H), 7.46 (m, 3H), 7.35 (m, IH), 7.26 (m, 3H), 7.19 (ddd, 1H, J= 0.9, 4.9, 5.6), 7.03 (m, 2H), 5.01 (m, 1H), 4.84 (d(br, IH, J = 7.4), 4.17 (q, 2H, J = 7.0), 4.08 (s, 2H), 3.24 (dd, IH, J = 5.9,
13.5) , 3.16 (dd, IH, J = 5.9, 13.6), 1.23 (t, 3H, J = 7.0). HRMS: calcd for C27H27N4O2, 439.2134; found, 439.2137.
Ethyl (3-(2-Methylbenzyl)-5-phenylpyrazin-2-yl)phenyIalaninate (N-74): Obtained from L-12 and PheOEt, HC1 as an oil (0.40 g, 86 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). ' II NMR (CDCI3): 8.43 (s, IH), 7.92 (m, 2H), 7.43 (m, 2H), 7.35 (m, IH), 7.20 (m, 5H), 7.11 (m, IH), 6.98 (m, 3H), 5.01 (m, IH), 4.86 (d (br), IH, J = 7.1), 4.14 (m, 4H), 3.17 (dd, 1 H, J= 5.6, 13.8), 3.07 (dd, IH, .7= 6.0, 13.8), 2.33 (s, 3H), 1.21 (t, 3H, J= 7.1). HRMS: calcd for C29H29N3O2, 452.2338; found, 452.2338. Ethyl 3-(furan-2-yl)-2-((3-(2-methyIhenzyl)-5-phenylp>Tazin-2-yl)amino)propanoate (N-75):
Obtained from L-12 and B-7 as an oil (0.30 g, 71 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.43 (s, 1H), 7.93 (m, 2H), 7.47 (m, 2H), 7.35 (m, 1H), 7.20 (m, 3H), 7.13 (m, 1 H), 7.06 (m, 1H), 6.21 (dd, IB, J = 1.8, 3.1), 5.85 (m, 1 H), 5.09 (d (br), 1H, J= 7.6), 4.98 (m, 1H), 4.18 (m, 4H), 3.20 (m, 2H), 2.39 (s, 3H), 1.23 (t, 3H, J= 7.1). HRMS: calcd for C27H28N303, 442.2131 ; found, 442.2137.
Ethyl (3-(3-methylbenzyl)-5-phenylpyrazin-2-yl)phenylalaninate (N-76): Obtained from L-13 and PheOEt, HC1 as an oil (0.41 g, 89 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.43 (s, 1H), 7.97 (m, 2H), 7.48 (m, 2H), 7.37 (m, 1H), 7.21 (m, 4H), 7.08-6.96 (m, 4H), 4.97 (m, 2H), 4.14 (m, 4H), 3.17 (dd, 1H, J = 5.0, 13.7), 3.07 (dd, III, J - 5.7, 13.7), 2.31 (s, 3H), 1.20 (t, 3H, J = 7.1). HRMS: calcd for C29H29N3O2, 452.2338; found, 452.2337.
Ethyl 3-(furan-2-yl)-2-((3-(3-methylbenzyl)-5-phenylpyrazin-2-yl)amino)propanoate (N-77): Obtained from L-13 and B-7 as an oil (0.35 g, 75 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDC13): 8.42 (s, 1 H), 7.96 (m, 2H), 7.48 (m, 2H), 7.38 (m, 1 H), 7.21 (m, 2H), 7.08 (m, 3H), 6.22 (dd, 1H, J = 2.0, 3.3), 5.83 (m, 1H), 5.16 (d (br), 1 H, J - 7.6), 4.96 (m, IB), 4.17 (m, 4H), 3.21 (m, 211), 2.33 (s, 3H), 1.21 (t, 3H, J= 7.1). HRMS: calcd for C27H28N3O3, 442.2131 ; found, 442.2133.
Ethyl (3-(2-iluorobenzyl)-5-phenylpyrazin-2-yl)phenylalaninate (N-78): Obtained from L-3 and PheOEt, HCl as an oil (0.19 g, 40 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 94/6). Ή NMR (CDC13, 400 MHz): 8.44 (s, 1 H), 7.94-7.91 (m, 2H), 7.48-7.44 (m, 2H), 7.38-7.34 (m, I H), 7.28-7.22 (m, 5H), 7.1 1-7.05 (m, 4H), 5.10-5.08 (m, 1H), 5.04-4.99 (m, IH), 4.20-4.09 (m, 4H), 3.28-3.23 (m, 1H), 3.18-3.13 (m, 1H), 1.23- 1.20 (t, 3H, J= 7.9). HRMS: calcd for C28H27FN302, 456.2087; found, 456.2096.
Ethyl 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoate (N-79): Obtained from L-3 and B-6 as an oil (0.34 g, 69 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). !H NMR (CDCI3, 400 MHz): 8.42 (s, IH), 7.91 (m, 2H), 7.45 (m, 2H), 7.36 (m, IH), 7.23 (m, 2H), 7.16-7.04 (m, 4H), 6.95 (m, I H), 6.88 (m, IH), 5.06 (d(br), IH, J = 7.3), 4.97 (m, IH), 4.15 (m, 4H), 3.20 (dd, IH, J = 5.5, 13.7), 3.10 (dd, IH, J = 6.7, 13.7), 2.31 (s, 311), 1.21 (t, 3H, J = 7.1). HRMS: calcd for C29H29FN3O2, 470.2244, found, 470.2253.
Ethyl 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoate (N-80): Obtained from L-3 and B-3 as an oil (0.32 g, 63 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 94/6). Ή NMR (CDCI3, 400 MHz): 8.42 (s, IH), 7.91 (m, 2H), 7.45 (m, 2H), 7.36 (m, 1H), 7.23 (m, 2H), 7.16 (m, 1H), 7.06 (m, 2H), 6.78 (m, 1 H), 6.68 (m, 2H), 5.08 (d(br), 1H, J= 7.2), 4.99 (m, 1 H), 4.16 (m, 4H), 3.77 (s, 3H), 3.23 (dd, 1 H, J = 5.6, 13.9), 3.12 (dd, 1 H, J = 6.4, 13.9), 1.22 (t, 3H, J - 7. 1 ). HEMS: calcd for C29H29FN3O3, 486.2193, found, 486.2182.
Ethyl 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-81 ): Obtained from L-3 and B-7 as an oil (0.29 g, 62 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3, 400 MHz): 8.42 (s, 1H), 7.92 (m, 2H), 7.45 (m, 2H), 7.36 (m, IH), 7.26 (m, 3H), 7.09 (m, 2H), 6.24 (dd, IH, J = 1.9, 3.2), 5.95 (d, 1H, J= 3.1), 5.28 (d(br), 1 H, J= 7.6), 5.01 (m, I H), 4.19 (m, 411), 3.77 (s, 3H), 3.26 (m, 2H), 1.24 (t, 3H, J= 7.1). HRMS: calcd for C26H25FN3O3, 446.1880, found, 446.1873.
Ethyl 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-82): Obtained from L-3 and B-8 as an oil (0.23 g, 48 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3, 400 MHz): 8.42 (s, IH), 7.91 (m, 2H), 7.45 (m, 2H), 7.36 (m, IH), 7.25 (m, 2H), 7.08 (m, 2H), 5.82 (m, 2H), 5.27 (d(br), IH, J= 7.6), 4.97 (m, IH), 4.19 (m, 4H), 3.77 (s, 3H), 3.20 (d, 2H, J= 5.8), 2.23 (s, 3H), 1.24 (t, 3H, J= 7.1). HRMS: calcd for C27H27FN3O3, 460.2036, found, 460.2044.
Ethyl 3-(5-ethyliuran-2-yl)-2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)propanoate (N-83): Obtained from L-3 and B-10 as an oil (0.19 g, 46 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDC , 400 MHz): 8.41 (s, IH), 7.91 (m, 2H), 7.46 (m, 2H), 7.34 (m, IH), 7.25 (m, 2H), 7.07 (m, 2H), 5.82 (m, 2H), 5.27 (d(br), 1 H, J - 7.3), 4.97 (m, IH), 4.20 (m, 4H), 3.77 (s, 3H), 3.20 (d, 2H, J = 5.1), 2.57 (q, 2H, J = 7.5), 1.25 (t, 3H, J = 7.1), 1.20 (t, 3H, J = 7.5). HRMS: calcd for C28H29FN3O3, 474.2193, found, 474.2198.
Ethyl (3-(2-chlorobenzyl)-5-phenylpyrazin-2-yl)phenylalaninate (N-84): Obtained from L-4 and PheOEt, HC1 as an oil (0.19 g, 68 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96/4)Ή NMR (CDCI3): 8.44 (s, IH), 7.91 (m, 2H), 7.44 (m, 3H), 7.35 (m, IH), 7.26-7.16 (m, 6H), 7.05 (m, 2H), 5.00 (m, 2H), 4.24 (m, 2H), 4.16 (dq, J= 7.2, 1.0 Hz, 2H), 3.22 (dd, J = 13.7, 5.4 Hz, IH), 3.13 (dd, J = 13.7, 6.3 Hz, IH), 1.22 (t, J = 7.2 Hz, 3H). HRMS: calcd for C28H27CIN3O2: 472.1792; found, 472.1784.
Ethyl 2-((3-(2-chlorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-85): Obtained from L-4 and B-7 as an oil (0.44 g, 65 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96/4). !H NMR (CDCI3): 8.44 (s, I H), 7.91 (m, 2H), 7.45 (m, 3H), 7.35 (m, IH), 7.25-7.15 (m, 4H), 6.22 (m, IH), 5.92 (m, IH), 5.18 (m, IH), 5.00 (m, IH), 4.28 (m, 211), 4.19 (q, J = 7.1 Hz, 2H), 3.24 (d, J = 5.5 Hz, 2H), 1.24 (t, J = 7.1 Hz, 3H). HRMS: calcd for C26H25CIN3O3: 462.1584; found, 462.1576.
Ethyl 2-((3-(2-chlorobcnzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-86): Obtained from L-4 and B-8 as an oil (0.11 g, 36 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95/5). Ή NMR (CDCI3): 8.43 (s, IB), 7.91 (m, 2H), 7.43 (m, 3H), 7.35 (m, 1 H), 7.20 (m, 3H), 6.22 (m, 1H), 5.80 (m, 2H), 5.19 (d (br), 1 H, J = 7.6), 4.98 (m, 1 H), 4.28 (m, 2H), 4.20 (m, 2H), 3.19 (m, 2H), 2.21 (s, 3H), 1.25 (t, J = 7.1 Hz, 3H). HRMS: calcd for C27H27CIN3O3: 476.1741 ; found, 476.1744.
Ethyl (3-(3-fluorobenzyl)-5-phenylp>Tazin-2-yl)phenylalaninate (N-87): Obtained from L-6 and PheOEt, HC1 as an oil (0.40 g, 57 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 94:6). Ή NMR (CDCI3): 8.45 (s, IH), 7.95 (m, 2H), 7.48 (m, 2H), 7.38 (m, I H), 7.36 - 7.21 (m, 4H), 7.10 - 6.92 (m, 5H), 5.02 (m, IB), 4.85 (d(br), IH, J = 7.5), 4.16 (q, 2H, J = 7.2), 4.11 (s, 2H), 3.21 (dd, IH, J = 5.4, 13.8), 3.13 (dd, IH, J = 5.9, 13.8), 1.22 (t, J = 7.1 Hz, 3H). HRMS: calcd for C28H27EN3O2, 456.2087; found, 456.2067. Ethyl 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoate (N-88): Obtained from L-6 and B-7 as an oil (0.22 g, 57 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.44 (s, IH), 7.95 (m, 2H), 7.48 (m, 2H), 7.37 (m, IH), 7.28 (m, 2H), 7.06 (m, I H), 6.96 (m, 2H), 6.24 (dd, 1H, J = 2.0, 3.2), 5.89 (dd, IH, J= 0.7, 3.2), 5.08 (d(br), 1 H, J = 7.5), 4.87 (m, IH), 4.18 (m, 4H), 3.24 (d, 2H, J = 5.3), 1.23 (t, J = 7.1 Hz, 3H). HRMS: calcd for C26H25FN3O3, 446.1880; found, 446.1883.
Ethyl 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoate (N-89): Obtained from L-6 and B-8 as an oil (0.24 g, 57 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.43 (s, IH), 7.93 (m, 2H), 7.47 (m, 2H), 7.37 (m, IH), 7.28 (m, IH), 7.06 (m, IH), 6.96 (m, 2H), 5.82 (m, IH), 5.78 (m, IH), 5.06 (d(br), IH, J = 7.9), 4.93 (m, IH), 4.18 (m, 4H), 3.20 (d, 2H, J = 5.2), 2.22 (s, 3H), 1.24 (t, 3H, J= 7.1). HRMS: calcd for C27H27FN303, 460.2036; found, 460.2039.
Ethyl 3-(5-ethylfuran-2-yl)-2-((3-(3-fluorobenzyl)-5-phenylp>Tazin-2-yl)amino)propanoate (N-90): Obtained from L-6 and B-10 as an oil (0.20 g, 90 % pure) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). !H NMR (CDCI3): 8.43 (s, IH), 7.94 (m, 2H), 7.47 (m, 2H), 7.37 (m, IH), 7.27 (m, IH), 7.06 (m, IH), 6.96 (m, 2H), 5.82 (m, IH), 5.78 (m, IH), 5.06 (d(br), IH, J - 7.7), 4.94 (m, IH), 4.18 (m, 4H), 3.20 (d, 2H, J= 5.1), 2.57 (q, 2H, J= 7.6), 1.22 (m, 6H). HRMS: calcd for C2gH29FN303, 474.2193; found, 474.2199. Ethyl 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoate (N-91 ): Obtained from L-6 and B-6 as an oil (0.35 g, 74 %) after a chromatography over silica gel (cyclohexane-efhyl acetate 95:5). Ή NMR (CDC13): 8.45 (s, IH), 7.94 (m, 2H), 7.47 (m, 2H),
7.37 (m, I H), 7.23 (m, 1H), 7.14 (m, 1H), 7.07 (m, 1H), 6.93 (m, 3H), 6.81 (m, IH), 4.96 (m, 1 H), 4.82 (d(br), IH, J = 7.4), 4.18 (m, 4H), 3.17 (dd, IH, J = 5.5, 13.7), 3.09 (dd, IH, J = 6.2, 13.7), 2.32 (s, 3H), 1.21 (t, 3H J = 7.1). HRMS: calcd for C29H29FN3O2, 470.2244; found, 470.2249.
Ethyl 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-rnethoxyphenyl)propanoate (N-92): Obtained from L-6 and B-3 as an oil (0.22 g, 45 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 95:5). Ή NMR (CDCI3): 8.44 (s, IH), 7.94 (m, 2H), 7.47 (m, 2H), 7.36 (m, IH), 7.19 (m, 2H), 6.93 (m, 3H), 6.79 (m, IH), 6.60 (m, 2H), 4.97 (m, IH), 4.81 (d(br), IH, J= 7.7), 4.18 (m, 4H), 3.77 (s, 3H), 3.19 (dd, 1H, J= 5.7, 13.8), 3.10 (dd, IH, J = 5.9, 13.8), 1.21 (t, 3H J = 7.1). HRMS: calcd for C29H29FN3Q3, 486.2193; found, 486.2209.
Ethyl (3-(4-fluorobenzyl)-5-phenylpyrazin-2-yl)phenylalaninate (N-93): Obtained from L-7 and PheOEt, HCl as an oil (0.12 g, 80 % pure) after a chromatography over silica gel (cyclohexane-ethyl acetate 94:6). Ή NMR (CDC13): 8.44 (s, IH), 7.96 (m, 2H), 7.48 (m, 2H),
7.38 (m, IH), 7.27 - 7.22 (m, 3H), 7.16 (m, 2H), 7.01 - 6.94 (m, 4H), 5.02 (m, IH), 4.88
(d(br), IH, J= 7.5), 4.16 (q, 2H, J= 7.0), 4.10 (s, 2H), 3.24 (dd, IH, J = 5.5, 13.8), 3.14 (dd, IH, J= 5.9, 13.8), 1.24 (t, J - 7.1 Hz, 3H). HRMS: calcd for C28H27FN3O2, 456.2087; found, 456.2072.
Ethyl 2-(3-(2-methoxybenzyl)-5-phenylpyrazin-2-ylamino)-3-phenylpropanoate (N-94):
Obtained from L-5 and PheOEt, HC1 as an oil (0.1 g, 28 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 96:4). ]H NMR (CDCI3): 8.37 (s, IH), 7.97 (m, 2H), 7.47 (m, 2H), 7.37 (m, 2H), 7.21 (m, 4H), 7.02 (m, 2H), 6.93 (m, IH), 6.85 (m, IH), 6.03 (d(br), IH, J = 7.7), 5.08 (m, IH), 4.17 (m, 4H), 3.72 (s, 3H), 3.20 (dd, IH, /= 6.0, 13.9), 3.12 (dd, IH, J = 6.2, 13.9), 1.21 (t, 3H, J = 7.2). HRMS: calcd for C29H29N3O3, 468.2287; found, 468.2298.
General procedure for the debenzylation of O-benzy!-bearing N-aryl a-aminoesters N.
The considered compound (0.53 mmol), ammonium formate (0.17 g, 2.64 mmol) and 10 % palladium over charcoal (30 mg, 0.026 mmol) were heated to reflux in ethanol (15 mL) for two hours. The suspension was filtered, the filtrate concentrated to dryness to give fairly clean deprotected products as described below.
Ethyl (3-benzyl-5-(4-hydrox>phenyl)p Tazin-2-yl)tyrosinate (N-95): Obtained from N-9 as a white solid (0.18 g, 72 %) after a chromatography over silica gel (dichloromethane - ethanol 97/3). Ή NMR (CDC½): 8.28 (s, IH), 7.79 - 7.72 (m, 2H), 7.29 - 7.19 (m, 3H), 7.19 - 7.12 (m, 2H), 6.88 - 6.75 (m, 4H), 6.66 - 6.59 (m, 2H), 4.92 - 4.75 (m, 2H), 4.13 (q, J = 7.1 Hz, 2H), 4.09 (s, 2H), 3.06 (del, J= 14.0, 5.4 Hz, 1H), 2.97 (dd, J= 14.0, 6.0 Hz, 1H), 1.19 (t, J= 7.1 Hz, 3H). HEMS: calcd for 0,8Η28Ν304, 470.2080; found, 470.2104.
Ethyl 2-((3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)amino)-3-(5-methylfuran-2- yl)propanoate (N-96): Obtained from N-29 as a glass (0.21 g, 81 %) after a chromatography over silica gel (cyclohexane - ethyl acetate 3/1). Ή NMR (CDC13): 8.29 (s, 1H), 7.76 (m, 2H), 7.28 (m, 5H), 6.84 (m, 2H), 5.98 (m, 1 H), 5.76 (d(br), 1H, J= 3), 5.69 (s (br), 1H), 5.69 (d (br), 1 H), 4.88 (m, 1 H), 4.17 (m, 2H), 4.14 (s, 211), 3.13 (dd, 2H, J= 3.0, 5.6), 2.23 (s, 3H), 1.23 (t, J= 7.1 Hz, 3H). HUMS: calcd for C27H28N304, 459.2112; found, 459.2108.
Ethyl (3-benzyl-5-phenylpyrazin-2-yl)tyrosinate (N-97): Obtained from N-65 as a white solid (0.24 g, 46 %) after a chromatography over silica gel (dichloromethane - ethanol 98.5/1.5). Ή NMR (CDCI3): 8.39 (s, 1 H), 7.98 - 7.88 (m, 2H), 7.50 - 7.40 (m, 2H), 7.38 ~~ 7.31 (m, 1 H), 7.31 - 7.22 (m, 3H), 7.22 - 7.17 (m, 2H), 6.84 - 6.76 (m, 2H), 6.67 - 6.59 (m, 2H), 4.96 (s, 1H), 4.94 - 4.86 (m, 2H), 4.12 (s, 2H), 4.19 - 4.07 (m, 2H), 3.13 - 3.03 (m, 1H), 3.03 - 2.92 (m, 1H), 1.19 (t, 3H, J = 7.1). HRMS: calcd for C28H28N303, 454.2131 ; found, 454.2104. General procedure for the hydrolysis of N-aryl α-aminoesters N into the corresponding N-aryl a-aminoacids O. In a round bottomed flask, the considered ester (0.0046 mol) and powdered sodium hydroxide (0.54 g, 0.0137 mol) were mixed. The atmosphere was replaced with argon and dry tetrahydrofuran (30 mL) was added. This was stirred overnight under an inert atmosphere, made acid with IN hydrochloric acid and extracted with ethyl acetate. The organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness to yield the corresponding acid as described below.
(3-Benzyl-5-phenylpyrazin-2-yl)phenylalanine (O-l): Obtained from N-5 as a hard foam (1.7 g, 91 %). Ή NMR (DMSO- ): 12.61 (s, 1H), 8.44 (s, HI), 7.90 (m, 2H), 7.40 (m, 2H), 7.32 - 7.15 (m, 1 1H), 6.84 (m, 1H), 4.66 (m, 1 H), 4.30 (d, 1H, J = 14.5), 4.06 (d, 1H, J = 14.5), 3.21 (m, 2H). HRMS: calcd for C26H24N302, 410.1869; found, 410.1875.
2-((3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(4-(benzyloxy)phenyl)propanoic acid (0-2): Obtained from intermediates N-8 or N-9 as a hard foam (0.42 g, 87 %). !H NMR (CDCI3): 8.31 (s, 1H), 7.88 - 7.81 (m, 2H), 7.49 - 7.29 (m, 11H), 7.24 - 7.16 (m, 3H), 7.13 - 7.08 (m, 2H), 7.08 - 7.01 (m, 2H), 6.95 - 6.87 (m, 2H), 6.86 - 6.79 (m, 2H), 5.1 1 (s, 2H), 5.03 (s, 2H), 4.86 - 4.76 (m, 1 H), 4.71 (d, 1H, J = 6.3), 4.06 (d, 1H, J = 15.4), 4.00 (d, 1H, J = 15.5), 3.19 (dd, IB, J = 14.2, 5.2), 3.01 (dd, 1H, J = 14.2, 6.9). HRMS: calcd for C40H36N3O4, 622.2706; found, 622.2763. (3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)phenylalanine (0-3): Obtained from N-66 as a foam still containing some ethyl acetate (3.73 g). Ή NMR (DMSO- ): 12.60 (s, IH), 8.36 (s, IH), 7.83 (in, 2H), 7.46 (m, 2H), 7.40 (m, 2H), 7.32 (m, IH), 7.29 - 7.15 (m, 9H), 7.04 (m, 2H), 6.71 (m, I H), 5.13 (s, 2H), 4.64 (m, IH), 4.28 (d, IH, J = 14.6), 4.03 (d, IH, J = 14.5), 3.21 (m, 2H). HRMS: calcd for CSSHBQNSOB, 516.2287; found, 516.2273.
2-((3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(3-propylphenyl)propanoic acid (0-4): This compound was obtained from N-70 as a glass (0.76 g) still containing some ethyl acetate. Ή NMR (CDC13): 8.33 (s, IH), 7.86 (m, 2H), 7.34 (m, 1 1 H), 7.09 (m, 5H), 6.96 (s, IH), 6.87 (m, IH), 4.81 (m, IH), 4.69 (m, I H), 4.07 (d, IH, J= 15.4), 3.93 (d, IH, J = 15.4), 3.27 (dd, 1 H, J = 14.2, 5.2), 3.05 (dd, IH, J= 14.2, 7.6), 2.56 (m, 2H), 1.62 (m, 2H), 0.93 (t, 3H, J= 7.3). HRMS: calcd for C36H36N3O3: 558.2757; found, 558.2746.
2-((3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid (O- 5): Obtained from N-67 as a foam still containing some ethyl acetate (0.8 g). ΊΙ NMR (CDCI3): 10.29 (s(br), I H), 8.33 (s, IH), 7.87 (m, 2H), 7.49 (m, 2H), 7.41 (m, 2H), 7.37 7.23 (m, 7H), 7.08 (m, 2H), 6.25 (dd, I H, J= 1.9, 3.4), 5.95 (dd, IH, J= 0.5, 3.4), 5.14 (s, 2H), 5.0 (d(br), IH, J -- 7.4), 4.85 (m, IH), 4.15 (m, 2H), 3.28 (dd, I H, J= 5.0, 15.4), 3.20 (dd, IH, J = 6.5, 15.0). HRMS: calcd for C3iH2gN3045 506.2080; found, 516.2109.
2-((3-benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(4,5-dimethy!furan-2- yl)propanoic acid (0-6): Obtained from N-69 as a foam still containing some ethyl acetate (0.61 g). Ή NMR (CDCI3): 9.16 (s(br), IH), 8.32 (s, IH), 7.87 (m, 2H), 7.49- 7.22 (m, 10H), 7.08 (m, 2H), 5.77 (s, IH), 5.14 (s, 2H), 5.01 (d(br), IH, J= 6.6), 4.76 (m, IH), 4.15 (m, 2H),
3.18 (dd, IH, J = 4.9, 15.3), 3.10 (dd, IH, J = 7.1 , 15.3), 2.14 (s, 3H), 1.89 (s, 311). HRMS: calcd for C33H32N3O4, 534.2393; found, 534.2410.
■ 2-((3-Benzyl-5-(4-(benzyloxy)phenyl)pyrazin-2-yl)amino)-3-(5-ethyliuran-2-yl)propanoic acid (0-7): Obtained from N-68 as a foam still containing some ethyl acetate (0.59 g). Ή NMR (CDCK): 9.16 (s(br), IH), 8.32 (s, IH), 7.87 (m, 2H), 7.47 (m, 2H), 7.41 (m, 2H), 7.37- 7.22 (m, 9H), 7.08 (m, 2H), 5.89 (m, 2H), 5.14 (s, 2H), 4.96 (d(br), IH, J = 6.4), 4.80 (m, IH), 4.12 (m, 2H), 3.23 (dd, IH, J = 4.9, 15.2), 3.14 (dd, IH, J = 7.1 , 15.2), 2.57 (q, 2H, J = 7.6), 1.19 (t, 3H, J= 7.6). HRMS: calcd for C33H32N3O4, 534.2393; found, 534.2405.
(3-Benzyl-5-(4-(benzyloxy)-3-fluorophenyl)pyrazin-2-yl)phenylalanine (0-8): Obtained from N-10 as a hard foam (0.56 g, 92 %). !H NMR (DMSO-</6): 12.61 (s, I H), 8.42 (s, IH), 7.73 (m, IH), 7.67 (m, IH), 7.47 (m, 2H), 7.40 (m, 2H), 7.34 (m, IH), 7.29 - 7.16 (m, 1 IH), 6.82 (d (br), I H, J= 8.2), 5.20 (s, 2H), 4.66 (m, IH), 4.27 (d, IH, J= 15.0), 4.02 (d, IH, J= 15.0),
3.19 (m, 2H). HRMS: calcd for C33H29FN3O3, 535.2225; found, 535.2239. General procedure for the debenzylation of O-benzyl-bearing N-aryl a-aminoacids O.
The considered O-bcnzyl derivative (1.5 mmol) and ammonium formate (2.8 g, 0.044 mol) were dissolved in isopropanol (50 mL). To this was added 10 % palladium over charcoal (0.16 g, 0.15 mmol) and the suspension was heated to reflux for one hour. This was cooled, filtered and concentrated to dryness under high vacuum. This led to a foam still containing some isopropanol. In. some cases some unreacted compound was detected and the crude residue was then subjected to a second round of hydrogenation. As described below some attempts were made to further purify the resulting amino acids (with rather big losses) but it turned out that as long as the crude compound has been subject to a thorough drying under high vacuum (to remove all the ammonium formate) it is suitable for use in the next step.
(3-Benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)tyrosine (0-9): The residue was obtained from 0-2, dispersed in water and filtered and the solid obtained was dried and dispersed again in 0.5 M hydrochloric acid, filtered and thoroughly dried again to give the pure compound (0.37 g, 45 %). Ή NMR (DMSO- ): 8.31 (s, 1 H), 7.72 (m, 2H), 7.22 (m, 5H), 7.02 (m, 2H), 6.82 (m, 2H), 6.61 (m, 2H), 6.54 (bs, I H), 4.54 (m, 1 H), 4.28 (d, 1H, J = 14.6), 4.02 (d, 1H, J = 14.6), 3.06 (m, 2H). HRMS: calcd for C26H24N3O4: 442.1767; found, 442.1773.
(3-Benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)phenylalanine (O-10): The residue was obtained from 0-3, dispersed in water and filtered and the solid obtained was dried and dispersed again in 0.5 M hydrochloric acid, filtered and thoroughly dried again to give the pure compound (2.06 g, 70 % from the corresponding ester). Ή NMR (DMSO-</6): 8.34 (s, 1 H), 7.72 (m, 2H), 7.20 (m, 5H), 7.08 (m, 5H), 6.82 (m, 2H), 6.34 (bs, I H), 4.36 (m, 1 H), 4.05 (d, IH, J = 14.8), 3.92 (d, 1H, J = 14.8), 3.25 (dd, 1H, J= 13.4, 4.7), 3.10 (dd, 1H, J= 13.4, 6.2). HRMS: calcd for C26H24N3O3: 426.1818; found, 426.1806.
2-((3-Benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)amino)-3-(3-propylphenyl)propanoic acid (O- 1 1): The residue was obtained from 0-4, dispersed in water, made basic with ammonia and extracted with ethyl acetate. The organic layer was dried over magnesium sulfate and concentrated to dryness to yield this compound as a glass (0.5 g, 71 % from the initial ester) still containing a small amount of ethyl acetate. Ή NMR (DMSO-<fc): 9.56 (s(br), IH), 8.38 (s, I H), 7.70 (m, 2H), 7.20 (m, 6H), 7.04 (m, 5H), 6.80 (m, 2H), 6.52 (m, IH), 4.61 (m, IH), 4.20 (d, IH, J= 14.9), 3.98 (d, 1H, J = 14.9), 3.14 (m, 2H), 2.44 (m, 2H), 1.50 (m, 2H), 0.83 (t, 3H, J= 7.3). HRMS: calcd for C29H30N3O3: 468.2287; found, 468.2286.
2-((3-Benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid (0-12): This compounds was obtained from 0-5 as a hard foam (0.54 g, 85 % from the initial ester N- 67) still containing a small amount of isopropanol after extensive drying under high vacuum. Ή NMR (DMSO- ): 9.56 (s(br), 1H), 8.34 (s, 1H), 7.73 (m, 2H), 7.46 (dd, 1H, J= 0.7, 1.8),
731-7.14 (m, 6H), 6.80 (m, 2H), 6.61 (d, 1H, J = 7.3), 6.30 (dd, III, J - 1.8, 3.1), 6.07 (dd, 1H, J = 0.7, 3.1), 4.67 (m, 1H), 4.20 (d, 1 H, J = 14.8), 4.04 (d, 1H, J = 143), 3.24 (m, 2H). HRMS: calcd for C24H22N30 : 416.1610; found, 416.1606.
2-((3-Benzyl-5-(4-hydroxyphenyl)p>Tazin-2-yl)amino)-3-(4,5-dirnethylfuran-2-yl)propanoic acid (0-13): The residue was obtained from 0-6, dispersed in water, made basic with ammonia and extracted with ethyl acetate. The organic layer was dried over magnesium sulfate and concentrated to dryness and purified by a chromatography over silica gel (cyclohexane - ethyl acetate 1/1 to 0/1) to give the compound as a glass still containing some ethyl acetate (0.21 g, 42 % from ester N-69). Ή NMR (DMSO- ): 12.4 (s, 1 H), 9.49 (s(br), 1H), 8.33 (s, 1H), 7.75 (m, 2H), 7.32-7.16 (m, 6H), 6.80 (m, 2H), 6.54 (d, 1H, J = 8.0), 5.82 (s, 1 H), 4.60 (m, 1H), 4.23 (d, 1H, J = 14.8), 4.04 (d, 1H, J = 14.8), 3.10 (in, 2H), 2.07 (s, 3H), 1.81 (s, 3H). HRMS: calcd for C26H26N304: 444.1923; found, 444.1937.
2-((3-Benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoic acid (0-14): The residue was obtained from 0-7, dispersed in water, made basic with ammonia and extracted with ethyl acetate. The organic layer was dried over magnesium sulfate and concentrated to dryness to yield the compound 80 % pure as a glass still containing some ethyl acetate (0.11 g). 1H NMR (DMSO-i/6): 9.54 (s(br), 1H), 8.29 (s, 1H), 7.75 (m, 2H), 7.41-7.16 (m, 6H), 6.80 (m, 2H), 5.79 (s(br), 2H), 4.37 (m, 1H), 4.08 (d, 1H, J = 14.5), 3.98 (d, 1H, J = 14.5), 3.24 (dd, 1H, J = 4.6, 15.2), 3.07 (dd, 1H, J = 6.6, 15.2), 2.45 (q, 2H, J = 7.6), 1.06 (t, 3H, J- 7.6). HRMS: calcd for C26H26N304: 444.1923; found, 444.1930.
(3-Benzyl-5-(3-fiuoro-4-hydroxyphenyl)pyrazin-2-yl)phenylalanine (0-15): This compounds was obtained from 0-8, using ethanol as a solvent, as a hard foam (0.43 g, 92 %) still containing a small amount of ethanol after extensive drying under high vacuum. Ή NMR (DMSO-< 6): 8.37 (s, 1H), 7.64 (m, 1H), 7.54 (m, 1H), 7.26- 7.13 (m, l iH), 6.99 (m, 1H), 6.61 (d (br), 1H, J = 7.4), 4.55 (m, 1H), 4.18 (d, 1H, J = 14.7), 3.98 (d, 1H, J = 14.7), 3.23 (dd, 1H, J = 4.7, 13.6), 3.13 (dd, 1H, J = 8.9, 13.6). HRMS: calcd for C26H23FN3O3: 444.1723; found, 444.1732.
General procedure for the synthesis of O-protected imidazo[l,2-a)pyrazines P from «- aminoesters N via steps o and p. In a seaiable vessel, the considered N-pyrazyl a-aminoester
(1.0 mmol) and sodium hydroxide (0.06 g, 1.5 mmol) were weighted. The air was replaced with argon and anhydrous THF (5 mL) was injected. This was stirred at 20 °C under an inert atmosphere overnight. Depending on the O-protecting group wanted, acetic anhydride (0.28 mL, 3.0 mmol) or the corresponding equivalents of pivalic anhydride was added. After stirring an additional three hours at room temperature, this was diluted in ethyl acetate, washed with water, brine and concentrated to dryness. The traces of acetic acid and acetic anhydride were removed by co-evaporation with toluene and then cyclohexane and the residue further purified as described below.
8-Benzyl-2-(sec-butyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-l): Obtained from N-l as a white solid (0.11 g, 48 %) after a recrystallization in n-heptane. Ή NMR (CDC13): 7.92 (m, 2H), 7.79 (s, 1 H), 7.66 (m, 2H), 7.47 (m, 2H), 7.39 (m, 1H), 7.32 (m, 2H), 7.23 (m, 1H), 4.63 (s, 2H), 2.89 (six, 1H, J = 7.5), 2.49 (s, 3H), 1.89 (m, 1 H), 1.74 (m, IH), 1.39 (d, 3H, J= 7.5), 0.93 (t, 3H, J = 7.5). HRMS: calcd for C25H26N302, 400.2025; found, 400.2024.
8-Benzyl-2-(cyclopentylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-2): Obtained from N-2 as a powder (0.21 g, 88 %) after a concentration to dryness. Ή NMR (CDCI3): 7.92 (m, 2H), 7.80 (s, 1 H), 7.65 (m, 2H), 7.47 (m, 2H), 7.39 (m, 1H), 7.32 (m, 2H), 7.23 (m, 1H), 4.63 (s, 2H), 2.78 (d, 2H, J = 7.4), 2.50 (s, 3H), 2.38 (m, IH), 1.80 (m, 2H), 1.64 (m, 4H), 1.29 (m, 2H). HRMS: calcd for C27H28N302, 426.2181 ; found, 426.2180.
8-Benzyl-2-(cyclohexylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-3): Obtained from N-3 as a powder (0.24 g, 72 %) after a recrystallization in n- heptane. Ή NMR (CDC13): 7.92 (m, 2H), 7.80 (s, IH), 7.65 (m, 2H), 7.47 (m, 211), 7.39 (m, IH), 7.32 (m, 2H), 7.23 (m, IH), 4.63 (s, 2H), 2.78 (d, 2H, J - 7.2), 2.50 (s, 3H), 1.87 (m, IH), 1.75 (m, 5H), 1.27 (m, 3H), 1.05 (m, 2H). HRMS: calcd for C28H30N3O2, 440.2338; found, 440.2326.
8-Benzyl-2-(bicyclo[2.2.1 ]heptan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P- 4): Obtained from N-4 as a powder (0.58 g, 52 %) as a mixture of two diastereoisomers after a recrystallization in n-heptane. Ή NMR (CDC13): 7.92 (m, 2H), 7.80 (s, 0.4H), 7.79 (s, 0.6H), 7.65 (m, 2H), 7.47 (m, 2H), 7.39 (m, IH), 7.32 (m, 2H), 7.23 (m, IH), 4.63 (m, 211), 2.85 - 2.57 (m, 2H), 2.51 and 2.50 (2s, 3H), 2.42 (m, 0.6H), 2.26 (m, IH), 2.1 1 (m, IH), 2.04 (m, 0.4H), 1.84 (m, 0.6H), 1.73 (m, 0.8H), 1.63 - 1.15 (m, 611), 0.82 (m, 0.6H). HRMS: calcd for C29H30N3O2, 452.2338; found, 452.2355.
2,8-dibenzyl-5-methyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-5): Obtained as a glass from N-6 (0.3 g, 76 %). Ή NMR (CDC13): Ή NMR (CDCI3): 7.62 (m, 2H), 7.51-7.38 (m, 511), 7.33-7.23 (m, 8H), 4.58 (s, 2H), 4.15 (s, 2H), 2.56 (s, 3H), 2.14 (s, 3H). HRMS: calcd for C29H26 3O2, 448.2025; found, 448.2020.
8-benzyl-2-(furan-2-ylmethyl)-6-phenyiimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-6): Obtained from N-34 as a beige solid (0.10 g, 36 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 4: 1). Ή NMR (CDCI3): 7.92 (m, 2H), 7.83 (s, IH), 7.63 (m, 2H), 7.47 (m, 2H), 7.42-7.30 (m, 4H), 7.23 (m, IH), 6.35 (m, IH), 6.16 (m, IH), 4.64 (s, 2H), 4.24 (s, 2H), 2.36 (s, 3H). HRMS: calcd for
Figure imgf000108_0001
424.1661; found, 424.1607.
2,8-Dibenzyl-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-7): Obtained from N-5 as a yellowish solid (0.17 g, 50 %) after a recrystallization from cyclohexane. !H NMR (CDCI3): 7.92 (in, 2H), 7.80 (s, IH), 7.64 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.33 (m, 5H), 7.25 (m, 2H), 4.65 (s, 2H), 4.23 (s, 2H), 2.18 (s, 3H). HRMS: calcd for CigH^Oi, 434.1869; found, 434.1825.
8-Benzyl-6-phenyl-2-(l -phenylethyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-8): Obtained from N-7 as a white powder after a recrystallization from n-heptane (0.12 g, 44 %). 'II NMR (CDCI3): 7.90 (m, 2H), 7.74 (s, IH), 7.68 (m, 2H), 7.46 (m, 2H), 7.40 (m, I H), 7.33 (m, 5H), 7.25 (m, 2H), 4.68 (d, IH, J= 13.8), 4.63 (d, IH, J= 13.8), 4.34 (q, IH, J= 6.7), 2.14 (s, 3H), 1.83 (d, 3H, J= 6.7). HRMS: calcd for C29H26N3G2, 448.2025; found, 448.2031.
8-Benzyl-2-phenethyl-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-9): Obtained from N-71 as a white powder after a recrystallization from n-heptane (0.22 g, 67 %). Ή NMR (CDCI3): 7.92 (m, 2H), 7.81 (s, I H), 7.64 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.35-7.21 (m, 8H), 4.65 (s, 2H), 3.12 (m, 4H), 2.42 (s, 3H). HRMS: calcd for C29H26N3O2, 448.2025; found, 448.2026.
8-Benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-10): Obtained from N-36 as a white solid (0.14 g, 24 %) after a chromatography over silica gel (cyclohexane-ethyl acetate 5: 1 ) and a recrystallization from cyclohexane. Ή NMR (CDCI3): 7.93 - 7.86 (m, 2H), 7.82 (s, I H), 7.64 - 7.57 (m, 2H), 7.49 - 7.41 (m, 2H), 7.41 - 7.35 (m,
IH) , 7.35 - 7.27 (m, 2H), 7.25 - 7.16 (m, IH), 6.03 - 5.97 (m, IH), 5.93 - 5.86 (m, IH), 4.62 (s, 2H), 4.17 (s, 2H), 2.34 (s, 3H), 2.26 (s, 3H). HRMS: calcd for C27H24N3O3, 438.1818; found, 438.1828.
8-Benzyl-2-((5-methylfuran-2-yI)methyl)-6-(p-tolyl)imidazo[l,2-a]pyrazin-3-yl acetate (P-
I I) : Obtained from N-37 as a white solid (0.15 g, 70 %) after a recrystallization from n- heptane. !H NMR (CDCI3): 7.82 - 7.79 (m, 3H), 7.62 (m, 2H), 7.33 - 7.20 (m, 5H), 6.01 (d, 1H, J = 1), 5.90 (m, IH), 4.62 (s, 2H), 4.17 (s, 2H), 2.41 (s, 3H), 2.35 (s, 3H), 2.28 (s, 2H). HRMS: calcd for C28H26N303, 452.1974; found, 452.1960.
8-Benzyl-2-((5-methylfuran-2-yl)methyl)-6-(m-tolyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P- 12) Obtained from N-38 as a white solid (0.1 g, 53 %) after a (slow) recrystallization from n- heptane. Ή NMR (CDCI3): 7.82 (s, I H), 7.73 (m, IH), 7.79 (m, IH), 7.37 - 7.20 (m, 5H), 6.01 (d, IH, J= 3), 5.91 (m, IH), 4.62 (s, 2H), 4.18 (s, 2H), 2.45 (s, 3H), 2.36 (s, 3H), 2.28 (s, 2H). HRMS: calcd for C28H26N3O3, 452.1974; found, 452.1981. 8-Benzyl-2-(furan-3-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-13): Obtained from N-35 as a beige solid (0.74 g, 67 %) after a recrystallization from n-heptane. H NMR
(CDCI3): 7.92 (m, 2H), 7.83 (s, IH), 7.63 (m, 2H), 7.47 (m, 2H), 7.40 (m, 211), 7.36 - 7.30 (m, 3H), 7.24 (m, IH), 6.38 (m, IH), 4.64 (s, 2H), 4.00 (m, 2H), 2.34 (s, 3H). HRMS: calcd for C26H22N3O3, 424.1661 ; found, 424.1688.
2-((l ,3-Dioxolan-2-yl)methyl)-8-benzyl-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-14):
Obtained from N-12 as a white solid (0.46 g, 76 %) after recrystallization from w-heptane. H
NMR (CDCI3): 7.92 (m, 2H), 7.85 (s, I H), 7.62 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.32 (m, 2H), 7.23 (m, IH), 5.29 (t, IH, J = 4.5), 4.64 (s, 2H), 3.97 - 3.86 (m, 4H), 3.22 (d, 2H, J = 4.5), 2.49 (s, 3H). HRMS: calcd for C25H24N3O3, 430.1767; found, 430.1762.
8-Benzyl-2-(4-(benzyloxy)benzyl)-6-(4-(benzyloxy)phenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-15): Obtained from N-9 as a brown solid (0. 27 g, 66 %) after a recrystallization from cyclohexane. Ή NMR (CDCI3): 7.84 (m, 211), 7.71 (s, I H), 7.61 (m, 2H), 7.48 - 7.29 (m, 12H), 7.25 - 7.20 (m, 3H), 7.06 (m, 2H), 6.94 (m, 2H), 5.14 (s, 2H), 5.09 (s, 2H), 4.62 (s,
MS: calcd for C42B36N304; 646.2706; found, 646.2728.
Figure imgf000109_0001
2-Benzyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-16): Obtained from N-32 as a yellow solid (0.22 g, 48 %) after a recrystallization from /z-heptane. lH NMR (CDCI3): 9.08 (m, IH), 7.94 - 7.90 (m, 3H), 7.50 (m, 2H), 7.43 (m, I H), 7.36 - 7.24 (m, 5H), 4.19 (s, 2H), 2.27 (s, 3H). HRMS: calcd for C2 iH i8N302, 344.1399; found, 344.1389.
2-Benzyl-8-methyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-17): Obtained from N-33 as a yellow solid (0.59 g, 88 %) after a recrystallization from n-heptane. !H NMR (CDC137.91 (m, 2H), 7.80 (m, IH), 7.48 (m, 2H), 7.41 (m, IH), 7.35 - 7.23 (m, 5H), 4.21 (s, 2H), 2.96 (m, 3H), 2.18 (s, 3H). HRMS: calcd for C22H20N3O2, 358.1556; found, 358.1544.
8-Benzyl-2-(furan-2-ylmethyl)-6-(2-methoxyphenyl)imidazo[ l ,2-a]pyrazin-3-yl acetate (P- 18): Obtained from N-40 as a beige solid (0.27 g, 68 %) after a recrystallization from n- heptane. !H NMR (CDCI3): 8.21 (s, IH), 8.05 (dd, J= 7.7, 1.8 Hz, IH), 7.59 (dd, J= 7.9, 0.9
Hz, 2H), 7.39 - 7.27 (m, 4H), 7.23 7.18 (m, IH), 7.09 (td, J= 7.6, 1.1 Hz, IH), 6.99 (dd, J =
8.3, 0.9 Hz, IH), 6.33 (dd, J = 3.2, 1.9 Hz, IH), 6.16 - 6.10 (m, IH), 4.60 (s, 2H), 4.22 (s, 2H), 3.87 (s, 3H), 2.32 (s, 3H). HRMS: calcd for C27H24N3O4, 454.1767; found, 454.1785. 8-benzyl-2-(furan-3-ylmethyl)-6-(2-methoxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-
19): Obtained from N-41 as a beige solid (0.15 g, 29 %) after a recrystallization from n- heptane. Ή NMR (CDC13): 8.21 (s, IH), 8.07 (dd, J = 7.7, 1.8 Hz, IH), 7.61 (d, J = 7.4 Hz,
2H), 7.41 - 7.27 (m, 5H), 7.22 (d, J = 7.4 Hz, IH), 7.15 - 7.06 (m, IH), 6.99 (d, J= 8.2 Hz, IH), 6.40 - 6.34 (m, IH), 4.61 (s, 2H), 3.98 (s, 2H), 3.88 (s, 3H), 2.30 (s, 3H). HEMS: calcd for C27H24N3O4, 454.1767; found, 454.1786.
2,8-Dibenzyl-6-(2-methoxyphenyl)imidazo[ l ,2-a]pyrazin-3-yl acetate (P-20): Obtained from N-39 as a beige solid (0.22 g, 53%) after a recrystallization from n-heptane. !H NMR (CDCI3): 8.17 (s, IH), 8.05 (dd, J - 7.7, 1.8 Hz, IH), 7.65 - 7.58 (m, 2H), 7.38 - 7.27 (m, 7H), 7.26 - 7.16 (m, 2H), 7.09 (td, J = 7.6, 1.0 Hz, IH), 6.98 (dd, J - 8.3, 0.7 Hz, I H), 4.62
(s, 2H), 4.21 (s, 2H), 3.86 (s, 3H), 2.15 (s, 3H). HRMS: calcd for C29H26N303, 464.1974; found, 464.1979.
8-Benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate (P-21):
Obtained from N-62 as white solid (0.27 g, 57 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.93 ~~ 7.86 (m, 2H), 7.81 (s, IH), 7.64 - 7.57 (m, 2H), 7.49 - 7.41 (m, 2H), 7.41 - 7.35 (m, IH), 7.33 - 7.27 (m, 2H), 7.24 - 7.18 (m, IH), 6.01 (d, J = 3.0 Hz, IH), 5.93 - 5.87 (m, IH), 4.62 (s, 2H), 4.17 (s, 2H), 2.61 (q, J = 7.5 Hz, 211), 2.33 (s, 3H), 1.22 (t, J = 7.5 Hz, 3H). HRMS: calcd for C gHieNsOs, 452.1974; found, 452.2014.
8-Benzyl-6-phenyl-2-(thiophen-2-ylmethyl)imidazo[l ,2-aJpyrazin-3-yl acetate (P-22): Obtained from N-59 as a white solid (0.37 g, 73 %) after a recrystallization from w-heptane. Ή NMR (CDCI3): 7.92 - 7.87 (m, 2H), 7.80 (s, IH), 7.65■■- 7.58 (m, 2H), 7.49 - 7.42 (m, 2H), 7.42 - 7.34 (m, IH), 7.34 - 7.27 (m, 2H), 7.25 - 7.20 (m, IH), 7.19 (dd, J= 5.1, 1.3 Hz, IH), 6.95 (dd, J -= 5.1, 3.5 Hz, IH), 6.93 - 6.88 (m, IH), 4.62 (s, 2H), 4.38 (d, J = 0.8 Hz, 2H), 2.30 (s, 3H). HRMS: calcd for C26H22N3O2S, 440.1433; found, 440.1486.
8-Benzyl-2-((3-methyUhiophen-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-y! acetate (P-
23) : Obtained from N-60 as a solid (0.37 g, 95 %). !H NMR (CDCI3): 7.92 (m, 2H), 7.79 (s, IH), 7.64 (m, 2H), 7.47 (m, 2H), 7.39 (m, IH), 7.32 (m, 2H), 7.23 (m, IH), 7.10 (d, 1 H, J = 5.2), 6.83 (d, IH, J= 5.2), 4.64 (s, 2H), 4.32 (s, 2H), 2.27 (s, 3H), 2.22 (s, 3H). HRMS: calcd for C27H24N3O2S, 454.1589; found, 454.15 1.
8-Benzyl-2-((5-ethylthiophen-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-
24) : Obtained from N-61 as a white solid (0.25 g, 56 %) after a recrystallization from n- heptane. !H NMR (CDCI3): 7.93 (m, 2H), 7.83 (s, I H), 7.65 (m, 2H), 7.47 (in, 2H), 7.40 (m, I H), 7.32 (m, 2H), 7.24 (m, IH), 6.72 (m, IH), 6.63 (m, IH), 4.64 (s, 2H), 4.32 (s, 2H), 2.82 (q, 2H, J= 7.6), 2.33 (s, 3H), 1.31 (t, 3H, J= 7.6). HR S: calcd for C28H26N302S, 468.1746; found, 468.1757.
8-Benzyl-6-phenyl-2-((5-(trifluoromethyl)furan-2-yl)methyl)imidazo[l,2-a]pyrazin-3-yl acetate (P-25): Obtained from N-64 as a white solid (0.07 g, 43 %) after a recrystallization from n-heptane. !H NMR (CDC13): 7.94 - 7.87 (m, 2H), 7.83 (s, 1 H), 7.63 - 7.57 (m, 2H),
7.49 - 7.42 (m, 2H), 7.42 - 7.35 (m, IH), 7.33 - 7.27 (m, 2H), 7.24 - 7.17 (m, 1 H), 6.73 (dd, IH, J = 3.3, 1.2), 6.21 (dd, IH, J= 3.4, 0.7), 4.61 (s, 2H), 4.23 (s, 2H), 2.40 (s, 3H). HRMS: calcd for C27H21F3N3O3, 492.1535; found, 492.1566.
8-benzyl-2-(2-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-26): Obtained from N-51 as a white solid (0.44 g, 96 %): ¾ NMR (CDCI3): 7.91 (m, 211), 7.76 (s, IH), 7.64 (m, 2H), 7.48 -7.18 (m, 10H), 4.64 (s, 2H), 4.24 (s, 2H), 2.32 (s, 3H), 2.06 (s, 3H). HRMS: calcd for C29H26N3O2, 448.2025; found, 448.2032.
8-Bcnzyl-2-(3-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-27): Obtained from N-52 as a beige solid (0.17 g, 61 %) after a recrystallization from « -heptane. Ή NMR (CDCI3): 7.92 - 7.85 (m, 2H), 7.77 (s, IH), 7.66 - 7.58 (m, 2H), 7.49 - 7.35 (m, 3H), 7.35 - 7.27 (m, 2H), 7.24 - 7.16 (m, 2H), 7.15 - 7.01 (m, 3H), 4.62 (s, 2H), 4.16 (s, 2H), 2.33 (s, 311), 2.17 (s, 3H). HRMS: calcd for C29H26N3O2, 448.2025; found, 448.2053.
8-Benzyl-2-(4-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-28): Obtained from N-53 as a white solid (0.21 g, 60 %) after a recrystallization from «-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.80 (s, IH), 7.64 (m, 2H), 7.49 -7.13 (m, 10H), 4.65 (s, 2H), 4.18 (s, 2H), 2.36 (s, 3H), 2.20 (s, 3H). HRMS: calcd for C29H26N3O2, 448.2025; found, 448.2034. 8-Benzyl-6-phenyl-2-(3-propylbenzyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-29): Obtained from N-54 as a white solid (0.14 g, 54 %) after a recrystallization from «-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.80 (s, I H), 7.64 (m, 2H), 7.47 (m, 2H), 7.40 (m, I H), 7.33 (m, 2H), 7.24 (m, 2H), 7.10 (m, 3H), 4.65 (s, 2H), 4.20 (s, 2H), 2.59 (m, 2H), 2.17 (s, 3H), 1.66 (m, 2H), 0.97 (t, 3H, J = 7.3). HRMS: calcd for C31 H30N3O2, 476.2338; found, 476.2346.
8-Benzyl-2-(3-cyclopropylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-30): Obtained from N-58 as a white solid (0.29 g, 65 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.79 (s, IH), 7.64 (m, 2H), 7.46 (m, 2H), 7.39 (m, IH), 7.33 (m, 2H), 7.23 (m, 2H), 7.06 (m, 2H), 6.96 (m, IH), 4.64 (s, 2H), 4.18 (s, 2H), 2.18 (s, 3H), 1.89 (m, IH), 0.95 (m, 2H), 0.70 (m, 2H). HRMS: calcd for C3iH28N302, 474.2181 : found, 474.2189.
8-Benzyl-6-phenyl-2-(4-propylbenzyl)imidazo[l,2-a]pyrazin-3-yl acetate (P-31): Obtained from N-55 as a white solid (0.25 g, 73 %) after a recrystallization from «-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.80 (s, 1H), 7.64 (m, 2H), 7.47 (m, 2H), 7.40 (m, 1H), 7.33 (m, 2H), 7.24 (m, 3H), 7.15 (m, 2H), 4.65 (s, 2H), 4.20 (s, 2H), 2.60 (m, 2H), 2.18 (s, 3H), 1.66 (m, 2H), 0.98 (t, J= 7.3 Hz, 3H). HRMS: calcd for C31H30N3O2, 476.2338; found, 476.2332. 8-Benzyl-2-(4-cyclopropylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-32): Obtained from N-57 as a white solid (0.29 g, 68 %) after a recrystaliization from /i-heptane. ]H NMR (CDCI3): 7.91 (m, 2H), 7.80 (s, I H), 7.64 (m, 2H), 7.46 (m, 2H), 7.40 (m, 1H), 7.33 (m, 2H), 7.22 (m, 3H), 7.04 (m, 2H), 4.64 (s, 2H), 4.17 (s, 2H), 2.18 (s, 3H), 1.92 (m, 1H), 0.94 (m, 2H), 0.68 (m, 2H). HRMS: calcd for C31H28N3O2, 474.2181; found, 474.2183.
8-Benzyl-2-(4-isopropylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-33): Obtained from N-56 as a white solid (0.16 g, 70 %) after a recrystaliization from «-heptane. !H NMR (CDCI3): 7.93 -■- 7.84 (m, 2H), 7.77 (s, 1H), 7.65 - 7.59 (m, 2H), 7.49 - 7.41 (m, 2H), 7.41 -
7.33 (m, 1H), 7.33 - 7.28 (m, 2H), 7.24 7.14 (m, 5H), 4.62 (s, 2H), 4.17 (s, 2H), 2.90 (hept,
1H, J = 6.9), 2.13 (s, 3H), 1.25 (d, 6H, J - 6.9). HRMS: calcd for C31H30N3O2, 476.2338; found, 476.2337.
8-Benzyl-2-(4-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-34): Obtained from N-46 as a beige solid (0.31 g, 70 %) after a recrystaliization from n-heptane. Ή NMR
(CDCI3): 7.94 7.86 (m, 2H), 7.79 (s, IH), 7.65 - 7.58 (m, 2H), 7.50 - 7.36 (m, 3H), 7.35 -
7.17 (m, 5H), 7.00 (dd, J = 9.8, 7.7 Hz, 2H), 4.62 (s, 2H), 4.15 (s, 2H), 2.25 (s, 3H). HRMS: calcd for C28H23FN3O2, 452.1774; found, 452.1776.
8-Benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-35): Obtained from N-46' as a white solid (0.13 g, 57 %) after a recrystaliization from 1-heptane. Ή NMR (CDCI3): 7.92 (m, 2H), 7.81 (s, 1 H), 7.63 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.33 - 7.22 (m, 4H), 7.06 (m, 2H), 6.95 (m, IH), 4.64 (s, 2H), 4.19 (s, 2H), 2.29 (s, 3H). HRMS: calcd for C2gH23FN302, 452.1774; found, 452.1778.
8-Benzyl-2-(2-fluorobenzyl)-6-phenylimidazo[l,2-a]p>Tazin-3-yl acetate (P-36): Obtained from N-47 as a white solid (0.22 g, 71 %) after a recrystaliization from w-heptane. ! H NMR (CDCI3): 7.90 (m, 2H), 7.81 (s, IH), 7.63 (m, 2H), 7.48 - 7.21 (m, 8H), 7.10 (m, 2H), 4.63 (s, 2H), 4.23 (s, 2H), 2.29 (s, 3H). HRMS: calcd for C28H23FN3O2, 452.1774; found, 452.1769. 8-Benzyl-2-(2,4-difluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-37): Obtained from N-48 as a white solid (0.13 g, 52 %) after a recrystaliization from ^-heptane. Ή NMR (CDCI3): 7.90 (m, 2H), 7.81 (s, IH), 7.63 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.32 (m, 2H), 7.23 (m, IH), 6.84 (m, 2H), 4.62 (s, 2H), 4.16 (s, 2H), 2.36 (s, 3H). HRMS: calcd for C28H22F2N3O2, 470.1680; found, 470.1686. 8-benzyl-2-(4-chlorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-38): Obtained from N-49 as a white solid (0.23 g, 62 %) after a rccrystallization from «-heptane. !H NMR (CDCI3): 7.92 (m, 2H), 7.81 (s, IH), 7.62 (m, 2H), 7.48 (m, 2H), 7.40 (m, IH), 7.28 (m, 7H), 4.63 (s, 2H), 4.14 (s, 2H), 2.27 (s, 3H). HRMS: calcd for C28H23CIN3O2, 468.1479; found, 468.1485.
8-benzyl-2-(4-bromobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-39): Obtained from 50 as a white solid (0.18 g, 49 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.92 (m, 2H), 7.80 (s, IH), 7.62 (m, 2H), 7.44 (m, 5H), 7.32 (m, 2H), 7.24 (m, IH), 7.18 (m, IH), 4.63 (s, 2H), 4.14 (s, 2H), 2.27 (s, 3H). HRMS: calcd for CasB^BrN^, 512.0974; found, 512.0950.
8-Benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P- 40): Obtained from N-43 as a beige solid (0.09 g, 45 %) after a recrystallization from n- heptane. Ή NMR (CDCI3): 7.94 - 7.86 (m, 2H), 7.82 (s, IH), 7.65 - 7.58 (m, 2H), 7.50 - 7.41 (m, 2H), 7.41 - 7.35 (m, IH), 7.34 - 7.27 (m, 2H), 7.25 · 7.16 (m, IH), 5.90 (s, IH), 4.62 (s, 2H), 4.13 (s, 2H), 2.34 (s, 3H), 2.17 (s, 3H), 1.91 (s, 3H). HRMS: calcd for C28H26N3O3, 452.1974; found, 452.1990.
8-Benzyl-2-((4,5-dimethylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-41): Obtained from N-44 as a white solid (0.14 g, 59 %) after a recrystallization from n- heptane. Ή NMR (CDC13): 7.91 (m, 2H), 7.82 (s, IH), 7.64 (m, 2H), 7.47 (m, 2H), 7.40 (m, IH), 7.33 (m, 2H), 7.24 (m, IH), 6.59 (s, IH), 4.64 (s, 2H), 4.26 (s, 2H), 2.35 (s, 3H), 2.31 (s, 3H), 2.09 (s, 3H). HRMS: calcd for C28H26 3O2S, 468.1746; found, 468.1758.
8-Benzyl-2-((4,5-dimethyloxazol-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-42): Obtained from N-45 as a white crystals (0.1 g, 34 %) after a recrystallization from n- heptane. !H NMR (CDCI3): 7.91 (m, 2H), 7.85 (s, IH), 7.47 (m, 2H), 7.39 (m, IH), 7.31 (m, 2H), 7.24 (m, IH), 4.62 (s, 2H), 4.28 (s, 2H), 2.41 (s, 3H), 2.21 (s, 3H), 2.08 (s, 3H). HRMS: calcd for C27H25N4O3, 453.1927; found, 453.1915.
8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-43): Obtained from N-24 as a wax (0.44 g, 95 %). Ή NMR (CDC13): 8.20 (dt, IH, J= 1.8, 8.0), 8.09 (s, IH), 7.61 (m, 2H), 7.37 - 7.24 (m, 5H), 7.15 (ddd, 1H, J = 1.8, 8.1 , 9.4), 5.91 (s, IH), 4.63 (s, 2H), 4.41 (s, 2H), 2.35 (s, 3H), 2.19 (s, 3H), 1.92 (s, 3H). HRMS: calcd for C28H25FN303, 470.1880; found, 470.1890.
8-Benzyl-6-phenyl-2-(pyridin-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-44): Obtained from N-42 as an oil (0.41 g, 91 %). Ή NMR (CDC13): 8.56 (m, I H), 7.92 (m, I H), 7.90 (m, IH), 7.83 (s, IH), 7.62 (m, 2H), 7.46 (m, 2H), 7.40 (m, IH), 7.32 (m, 3H), 7.17 (m, 3H), 4.63 (s, 2H), 4.38 (s, 2H), 2.35 (s, 3H). HRMS: calcd for C27H23N4O2, 435.1821; found, 435.1823.
8-Benzyl-2-(2-methoxybenzyI)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-45): Obtained from N-13 as a white solid (0.23 g, 72 %) after a recrystaliization from «-heptane. Ή NMR (CDCI3): 7.90 (m, 2H), 7.78 (s, IH), 7.64 (m, 2H), 7.46 (m, 2H), 7.39 (m, IH), 7.32 (m, 111), 7.24 (m, 3H), 6.92 (m, 2H), 4.63 (s, 2H), 4.22 (s, 2H), 3.82 (s, 3H), 2.16 (s, 3H). HRMS: calcd for C29H26N3O3, 464.1974; found, 464.1978.
8-Benzyl-2-(3-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-46): Obtained from N-14 as a white solid (0.20 g, 56 %) after a recrystaliization from /2-heptane. Ή NMR (CDCI3): 7.92 - 7.86 (m, 2H), 7.78 (s, IH), 7.65 - 7.58 (m, 2H), 7.49 - 7.41 (m, 2H), 7.41 - 7.34 (m, IH), 7.33 - 7.27 (m, 2H), 7.25 - 7.18 (m, 2H), 6.91 - 6.86 (m, IH), 6.86 - 6.83 (m, IH), 6.81 - 6.75 (m, IH), 4.63 (s, 2H), 4.17 (s, 2H), 3.77 (s, 3H), 2.21 (s, 3H). HRMS: calcd for C29H26N3O3, 464.1974; found, 464.1982.
8-Benzyl-2-(4-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-47): Obtained from N- 15 as a white solid (0.04 g, 28 %) after a recrystaliization from «-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.80 (s, I H), 7.63 (m, 2H), 7.45 (m, 2H), 7.40 (m, IH), 7.33 (m, 3H), 7.23 (m, 2H), 6.87 (m, 2H), 4.63 (s, 2H), 4.17 (s, 2H), 3.77 (s, 3H), 2.21 (s, 3H). HRMS: calcd for C29H26N3O3, 464.1974; found, 464.1990.
8-Benzyl-6-phenyl-2-((tetrahydrofuran-2-yl)methyl)imidazo[] ,2-a]pyrazin-3-yl acetate (P- 48): Obtained from N-l l as an oil (0.36 g, 81 %). !H NMR (CDCI3): 7.93 - 7.87 (m, 2H), 7.82 (s, IH), 7.62 - 7.57 (m, 2H), 7.48 - 7.41 (m, 2H), 7.40 - 7.34 (m, IH), 7.32 - 7.27 (m, 2H), 7.23 - 7.17 (m, IH), 4.66 - 4.55 (m, 2H), 4.32 - 4.23 (m, IH), 3.89 - 3.69 (m, 2H), 3.10 - 2.95 (m, 2H), 2.46 (s, 3H), 2.09 - 1.98 (m, IH), 1.92 - 1.82 (m, 2H), 1.77 - 1.66 (m, I H). HRMS: calcd for C26H26N3O3, 428.1974; found, 428.1956.
2,8-Dibenzyl-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-49): Obtained from N- 19 as a solid (0.21 g, 45 %) after a recrystaliization from «-heptane. Ή NMR (CDCI3): 8.20 (m, IH), 8.05 (s, I H), 7.62 (m, 2H), 7.32 (m, 10H), 7.15 (m, IH), 4.63 (s, 2H), 4.22 (s, 2H), 2.18 (s, 3H). HRMS: calcd for C28H23F 3O2, 452.1774; found, 452.1838.
8-Benzyl-2-(4-fluorobenzyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3-yI acetate (P-50): Obtained from N-20 as a solid (0.1 g, 63 %) after a recrystaliization from n-heptane. !H NMR (CDCI3): 8.20 (dt, IH, J = 2.0, 7.9), 8.06 (s, I H), 7.60 (m, 2H), 7.38-7.21 (m, 7H), 7.15 (m, IH), 7.01 (m, 2H), 4.62 (s, 2H), 4.16 (s, 2H), 2.26 (s, 3H). HRMS: calcd for C28H22F2N302, 470.1680; found, 470.1670. 8-Benzyl-6-(2-fluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-51):
Obtained from N-21 as a solid (0.15 g, 36 %) after a recrystallization from « -heptane. Ή NMR (CDCI3): 8.20 (m, IB), 8.10 (s, IH), 7.61 (m, 2H), 7.31 (m, 6H), 7.14 (m, IH), 6.35 (m, IH), 6.16 (in, IH), 4.63 (s, 2H), 4.24 (s, 2H), 2.35 (s, 3H). HRMS: calcd for C26H2iFN303, 442.1567; found, 442.1631.
8-Benzyl-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)irnidazo[l ,2-a]pyrazin-3-yl acetate (P-52): Obtained from N-22 as a glass (0.08 g, 72 %). !H NMR (CDCI3): 8.19 (dt, IH, J = 1.8, 7.8), 8.09 (s, IH), 7.61 (m, 2H), 7.37-7.21 (m, 5H), 7.15 (ddd, 1H, J= 1.2, 8.1, 12.0), 6.02 (d, IH, J = 3.0), 5.90 (m, IH), 4.63 (s, 2H), 4.18 (s, 2H), 2.35 (s, 3H), 2.27 (s, 3H). HRMS: calcd for C27H23FN303, 456.1723; found, 456.1734.
8-Benzyl-2-((5-ethylfuran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-53): Obtained from N-23 as a wax (0.18 g, 95 %). ¾ NMR (CDC13): 8.19 (dt, IH, J= 1.8, 7.8), 8.10 (s, IH), 7.62 (m, 2H), 7.39-7.22 (m, 5H), 7.15 (ddd, IH, J = 1.2, 8.1, 12.0), 6.04 (d, IH, J = 3.0), 5.93 (m, IH), 4.64 (s, 2H), 4.21 (s, 2H), 2.65 (q, 211, J = 7.6), 2.34 (s, 3H), 1.24 (t, 3H, J= 7.6). HRMS: calcd for C2SH25FN303, 470.1880; found, 470.1884.
8-Benzyl-6-(3-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-54): Obtained from N-25 as white powder after a recrystallization in n-heptane (0.018 g, 14 %). Ή NMR (CDCI3): 7.85 (s, IH), 7.68 (m, 2H), 7.60 (m, 2H), 7.46-7.37 (m, 2H), 7.34-7.21 (m, 3H), 6.02 (d, 1H, J = 3.1), 5.90 (m, IH), 4.62 (s, 2H), 4.18 (s, 2H), 2.37 (s, 3H), 2.26 (s, 3H). HRMS: calcd for C27H23FN3O3, 456.1723; found, 456.1732.
8-Benzyl-6-(4-fluorophenyl)-2-((5-rnethylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-55): Obtained from N-26 as a glass (0.1 g, 83 %). Ή NMR (CDCI3): 7.88 (m, 2H), 7.78 (s, IH), 7.59 (m, 2H), 7.34-7.12 (m, 6H), 6.02 (d, IH, J = 3.0), 5.90 (m, IH), 4.61 (s, 2H), 4.18 (s, 2H), 2.36 (s, 3H), 2.27 (s, 3H). HIMS: calcd for C27H23FN3O3, 456.1723; found, 456.1721.
2,8-dibenzyl-6-(2,6-difluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-56): Obtained from
N-30 as a solid (0.16 g, 61 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.66 (s, IH), 7.63 (m, 2H), 7.31 (m, 9H), 7.02 (m, 2H), 4.62 (s, 2H), 4.21 (s, 2H), 2.13 (s, 3H). HRMS: calcd for
Figure imgf000115_0001
470.1680; found, 470.1630.
8-benzyl-6-(2,6-difluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-
57): Obtained from N-31 as wax (0.19 g, 95 %). Ή NMR (CDCI3): 7.70 (s, IH), 7.60 (m, 2H), 7.37-7.19 (m, 6H), 7.02 (m, 2H), 6.35 (m, IH), 6.15 (m, IH), 4.62 (s, 2H), 4.24 (s, 2H), 2.31 (s, 3H). HRMS: calcd for C26H19F2N3O3, 460.1473; found, 460.1430. 8-Benzyl-6-phenyl-2-(2-(trifluoromethyl)benzyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-58):
Obtained from N-16 as wax (0.07 g, 95 %). Ή NMR (CDC13): 7.93 (m, 2H), 7.80 (s, IH), 7.65 (m, 3H), 7.45 (m, 2H), 7.53 -- 7.21 (m, 9H), 4.65 (s, 2H), 4.38 (s, 2H), 2.28 (s, 3H). HRMS: calcd for C29H23F3N3O2, 502.1742; found, 502.1722.
8-Benzyl-6-phenyl-2-(3-(trifluoromethyl)benzyl)imidazo[l,2-a]p Tazin-3-yl acetate (P-59): Obtained from N-17 as white solid after a recrystallization in n- heptane (0.08 g, 57 %). Ή NMR (CDCI3): 7.91 (m, 2H), 7.81 (s, IH), 7.63 (m, 3H), 7.54-7.38 (m, 6H), 7.34 ^ 7.21 (m, 3H), 4.63 (s, 2H), 4.25 (s, 2H), 2.27 (s, 3H). HRMS: calcd for C29H23F3N3O2, 502.1742; found, 502.1795.
8-Benzyl-6-phenyl-2-(4-(trifluoromethyl)benzyl)imidazo[ l,2-aJpyrazin-3-yl acetate (P-60):
Obtained from N-18 as white solid after a recrystallization in n-heptane (0.08 g, 66 %). Ή
NMR (CDCI3): 7.92 (m, 2H), 7.81 (s, IH), 7.60 (m, 4H), 7.49-7.38 (m, 5H), 7.33 7.22 (m,
3H), 4.63 (s, 2H), 4.24 (s, 2H), 2.27 (s, 3H). HRMS: calcd for C29H23F3N3O2, 502.1742; found, 502.1730.
2,12-dibenzyl-5H-chromeno[4,3-e]imidazo[l ,2-a]p>Tazin-3-yl acetate (P-61): Obtained from
N-72 as a wax (0.06 g, 95 %). 5H NMR (CDCI3): 8.14 (dd, IH, J = 1.6, 7.8), 7.61 (s, IH),
7.60 (s, IH), 7.38-7.20 (m, 9H), 7.10 (dt, 1H5 J= 1.0, 8.4), 6.90 (d, 1H, J= 8.0), 5.58 (s, 2H),
4.61 (s, 2H), 4.15 (s, 2H), 2.14 (s, 3H). HRMS: calcd for C29H24N3O3, 462.1818; found, 462.1812.
8-benzyl-6-(4-methox pheny))-2-((5-methylfuran-2-yl)rnethyl)imidaz()[ 1 ,2-a]pyrazin-3-yl acetate (P-62): Obtained from N-27 as white solid after a recrystallization in n-heptane (0.11 g, 69 %). Ή NMR (CDCI3): 7.84 (m, 2H), 7.75 (s, IH), 7.61 (m, 2H), 7.32 (m, 2H), 7.24 (m, IH), 7.0 (m, 2H), 6.02 (m, IH), 5.91 (m, IH), 4.62 (s, 2H), 4.17 (s, 2H), 3.87 (s, 3H), 2.35 (s, 3H), 2.28 (s, 3H). HRMS: calcd for C29H26 3O4, 468.1923; found, 468.1919.
8-benzyl-6-(3-methoxyphenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-63): Obtained from N-28 as a wax (0.08 g, 95 %). !H NMR (CDCI3): 7.84 (s, IH), 7.61 (m, 2H), 7.52 (m, IH), 7.45 (m, IH), 7.38-7.29 (m, 3H), 7.23 (m, IH), 6.93 (m, IH), 6.02 (d, 1H, J= 3), 5.91 (m, IH), 4.63 (s, 2H), 4.18 (s, 2H), 3.88 (s, 3H), 2.35 (s, 3H), 2.28 (s, 3H). HRMS: calcd for C29H26 3O4, 468.1923; found, 468.1928.
8-benzyl-2-((5-cyclopropylfuran-2-yl)rnethyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P- 64): Obtained from N-63 as a white solid after a recrystallization in n-heptane (0.13 g, 42 %).
Ή NMR (CDC½): 7.93 - 7.86 (m, 2H), 7.81 (s, IH), 7.64 7.57 (m, 2H), 7.48 - 7.41 (m,
2H), 7.41 - 7.35 (m, IH), 7.34 - 7.27 (m, 2H), 7.24 -■· 7.17 (m, IH), 5.98 (d, 1H, J= 3.1), 5.86 (d, IH, J= 3.1), 4.61 (s, 2H), 4.15 (s, 2H), 2.34 (s, 3H), 1.84 (tt, IH, J= 8.4, 5.1), 0.89 - 0.80 (m, 2H), 0.76 - 0.69 (m, 2H). HRMS: calcd for C29H26N3O3, 464.1974; found, 464.1990. 2-Benzyl-6-phenyl-8-(pyridin-3-ylmethyl)imidazo[l,2-a]pyrazin-3-yl (P-65): Obtained from N-73 as white solid after a recrystallization in n-heptane (0.1 g, 37 %). !H NMR (CDCI3): 8.87 (d, IH, J - 2.0), 8.50 (dd, IH, J - 1.7, 5.0), 7.92 (m, IH), 7.87 (m, 2H), 7.82 (s, IH), 7.48-7.22 (m, 9H), 4.63 (s, 2H), 4.21 (s, 2H), 2.19 (s, 3H). HRMS: calcd for C27H22N4O2, 435.1821 ; found, 435.1805.
2-Benzyl-8-(2-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-66): Obtained from N-74 as a white solid (0.29 g, 77 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.87 (m, 2H), 7.82 (s, IH), 7.57 (m, IH), 7.44 (m, 2H), 7.37 (m, IH), 7.33 (m, 4H), 7.27 (m, I H), 7.22 (m, I H), 7.17 (m, 2H), 4.67 (s, 2H), 4.22 (s, 2H), 2.61 (s, 3H), 2.20 (s, 3H). HRMS: calcd for C29H26N3O2: 448.2025; found, 448.2031.
2-(Furan-2-yImethyl)-8-(2-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-67): Obtained from N-75 as a white solid (0.18 g, 64 %) after a recrystallization in n-heptane. Ή NMR (CDCI3): 7.88 (m, 2H), 7.85 (s, IH), 7.54 (m, 2H), 7.45 (m, 2H), 7.38 (m, 2H), 7.23 (m, I H), 7.16 (m, 2H), 6.36 (dd, I H, J = 1.9, 3.4), 6.17 (m, IH), 4.66 (s, 2H), 4.24 (s, 2H), 2.60 (s, 3H), 2.36 (s, 3H). HRMS: calcd for C27H24N3O3, 438.1818; found, 438.1823.
2-Benzyl-8-(3-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-68): Obtained from N-76 as a white solid (0.27 g, 70 %) after a recrystallization from n-heptane. !H NMR (CDCI3): 7.92 (m, 2H), 7.80 (s, IH), 7.57 (m, IH), 7.50-7.37 (m, 5H), 7.32 (m, 4H), 7.28-7.20 (m, 2H), 7.05 (m, IH), 4.61 (s, 2H), 4.22 (s, 2H), 2.35 (s, 3H), 2.19 (s, 3H). HRMS: calcd for C29H26N3O2: 448.2025; found, 448.2026.
2-(Furan-2-ylmethyl)-8-(3-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-69): Obtained from N-77 as a white solid (0.23 g, 70 %) after a recrystallization in n-heptane. Ή NMR (CDCI3): 7.93 (m, 2H), 7.84 (s, IH), 7.49-7.37 (m, 6H), 7.22 (m, I H), 7.05 (m, IH), 6.35 (dd, I H, J - 1.7, 3.1), 6.16 (m, IH), 4.60 (s, 211), 4.24 (s, 2H), 2.36 (s, 3H), 2.35 (s, 3H). HRMS: calcd for C27H.4N3O3, 438.1818; found, 438.1820.
2-Benzyl-8-(2-fluorobenzyl)-6-phenyIimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-70): Obtained from N-78 as white solid after a recrystallization in n- heptane (0.12 g, 61 %). Ή NMR (CDCI3): 7.86-7.83 (m, 3H), 7.52-7.48 (dt, 1H, J = 7.4, 1.9), 7.47-7.23 (m, 8H), 7.14-7.08 (m, 2H), 4.72 (s, 2H), 4.23 (s, 2H), 2.19 (s, 3H). HRMS: calcd for C28H22FN3O2, 452.1774, found, 452.1774.
8-(2-Fluorobenzyl)-2-(3-methylbenzyI)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-71 ): Obtained from N-79 as white solid after a recrystallization in n-heptane (0.18 g, 58 %). Ή NMR (CDCI3): 7.84 (m, 2H), 7.83 (s, IH), 7.50 (m, IH), 7.42 (m, 2H), 7.36 (m, IH), 7.23 (m, 2H), 7.13-7.06 (m, 511), 4.72 (s, 2H), 4.19 (s, 2H), 2.35 (s, 3H), 2.19 (s, 3H). HRMS: calcd for C29H25FN3O2, 466.1931, found, 466.1938.
8-(2-Fluorobenzyl)-2-(3-methoxybenzyl)-6-phcnylimidazo[l ,2-a]pyrazin-3-yl acetate (P-72): Obtained from N-80 as white solid after a recrystaiiization in n- heptane (0.26 g, 87 %). !H NMR (CDCI3): 7.85 (m, 2H), 7.83 (s, IH), 7.48 (m, IH), 7.42 (m, 2H), 7.36 (m, IH), 7.25 (m, 2H), 7.10 (m, 2H), 6.90 (m, 2H), 6.81 (m, IH), 4.71 (s, 2H), 4.19 (s, 2H), 3.80 (s, 3H), 2.23 (s, 3H). HRMS: calcd for C29H25FN3O3, 482.1880, found, 482.1873.
8-(2-fluorobcnzy])-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-73): Obtained from N-81 as white solid after a recrystaiiization in n- heptane (0.19 g, 73 %). Ή
NMR (CDCI3): 7.87 (s, IH), 7.85 (m, 2H), 7.50-7.34 (m, 5H), 7.24 (m, IH), 7.10 (m, 2H),
6.35 (dd, IH, J = 1.9, 3.2), 6.17 (dd, IH, J= 0.7, 3.2), 4.70 (s, 2H), 4.24 (s, 2H), 2.37 (s, 3H).
HRMS: calcd for Cie^FNsOa, 442.1567, found, 442.1567.
8-(2-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyraz
acetate (P-74): Obtained from N-82 as white solid after a recrystaiiization in n-heptane (0.08 g, 38 %). Ή NMR (CDCI3): 7.87 (s, IH), 7.85 (m, 2H), 7.50-7.34 (m, 4H), 7.24 (m, IH), 7.10
(m, 2H), 6.04 (d, IH, J = 3.0), 5.91 (m, IH), 4.70 (s, 2H), 4.19 (s, 2H), 2.37 (s, 3H), 2.28 (s,
3H). HRMS: calcd for C27H23FN3O3, 456.1723, found, 456.1719.
2-((5-Ethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylirnidazo[l ,2-a]pyrazin-3-yl acetate (P-75): Obtained from N-83 as white solid after a recrystaiiization in n-heptane (0.05 g, 28
%). !H NMR (CDCI3): 7.87 (s, IH), 7.85 (in, 2H), 7.50-7.34 (m, 4H), 7.24 (m, IH), 7.10 (m,
2H), 6.04 (d, IH, J= 3.1), 5.92 (m, IH), 4.70 (s, 2H), 4.20 (s, 2H), 2.64 (q, 2H, J= 7.2), 2.36
(s, 3H), 1.24 (t, 3H, J= 7.2). HRMS: calcd for C28H25FN3O3, 470.1880, found, 470.1881.
2-Benzyl-8-(2-chlorobenzyl)-6-phenyliraidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-76): Obtained from N-84 as a white solid after a recrystaiiization in n-heptane (0.12 g, 67 %). 5il NMR
(CDCI3): 7.84 (s, IH), 7.83 (m, 2H), 7.50-7.20 (m, 12H), 4.82 (s, 2H), 4.23 (s, 2H), 2.20 (s,
3H). HRMS: calcd for C28H23CI 3O2: 468.1479; found, 468.1489.
8-(2-Chlorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-77): Obtained from N-85 as a white solid after a recrystaiiization in n-heptane (0.27 g, 70 %). lH NMR (CDCI3): 7.88 (s, IH), 7.83 (m, 2H), 7.46-7.34 (m, 6H), 7.23 (m, 2H), 6.36 (m, IH), 6.18 (m, IH), 4.81 (s, 2H), 4.25 (s, 2H), 2.38 (s, 3H). HRMS: calcd for C26H20CIN3O3:
458.1271; found, 458.1266.
8-(2-chlorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-78): Obtained from N-86 as a solid after a recrystaiiization in n-heptane (0.03 g, 27 %). Ή NMR (CDCI3): 7.88 (s, I H), 7.83 (m, 2H), 7.46-7.33 (m, 5H), 7.21 (m, 2H), 6.04 (m, III), 5.91 (m, IH), 4.81 (s, 2H), 4.19 (s, 2H), 2.38 (s, 3H), 2.28 (s, 3H). HRMS: calcd for C27H24CIN3O3: 472.1428; found, 472.1408.
2-Benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-79): Obtained from N-87 as a solid (0.19 g, 50 %) after a recrystallization from n-heptane. !H NMR (CDCI3): 7.90 (m, 2H), 7.82 (s, IH), 7.47 (m, 2H), 7.42 - 7.25 (m, 9H), 6.95 (m, IH), 4.63 (s, 2H), 4.22 (s, 2H), 2.20 (s, 3H). HRMS: calcd for C28H23FN3O2, 452.1774; found, 452.1775. 8-(3-Fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate (P-80): Obtained from N-91 as a solid (0.16 g, 50 %) after a recrystallization from «-heptane. lH NMR (CDCI3): 7.91 (m, 2H), 7.82 (s, IH), 7.47 (m, 2H), 7.38 (m, 3H), 7.28 (m, IH), 7.22 (m, IH), 7.09 (m, 3H), 6.95 (m, IH), 4.63 (s, 2H), 4.18 (s, 2H), 2.35 (s, 3H), 2.20 (s, 3H). HRMS: calcd for C29H25FN3O2, 466.1931; found, 466.1926.
8-(3-Fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-81): Obtained from N-92 as a solid (0.12 g, 60 %) after a recrystallization from «-heptane. Ή NMR (CDCI3): 7.90 (m, 2H), 7.82 (s, IH), 7.47 (m, 2H), 7.38 (m, 3H), 7.26 (m, 2H), 6. 95 (m, 3H), 6.80 (m, I H), 4.63 (s, 2H), 4.19 (s, 2H), 3.79 (s, 3H), 2.24 (s, 3H). HRMS: calcd for C29H25FN3O3, 482.1880; found, 482.1879.
8-(3-Fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-82): Obtained from N-88 as a solid (0.13 g, 59 %) after a recrystallization from n-heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.85 (s, IH), 7.49 (m, 2H), 7.42 - 7.33 (m, 4H), 7.26 (m, IH), 6.93 (m, IH), 6.36 (dd, IH, J- 2.0, 3.2), 6.17 (dd, I H, J - 0.8, 3.2), 4.62 (s, 2H), 4.24 (s, 2H), 2.36 (s, 3H). HRMS: calcd for C26H2iFN303, 442.1567; found, 442.1549.
8-(3-Fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-83): Obtained from N-89 as a solid (0.10 g, 45 %) after a recrystallization from n- heptane. Ή NMR (CDCI3): 7.91 (m, 2H), 7.85 (s, IH), 7.47 (m, 2H), 7.42 - 7.24 (m, 4H), 6.92 (m, I H), 6.03 (d, IH, J= 3.0), 5.91 (m, IH), 4.62 (s, 2H), 4.18 (s, 2H), 2.37 (s, 3H), 2.28 (s, 3H). HRMS: calcd for C27H23FN3O3, 456.1723; found, 456.1732.
2-((5-Ethylfuran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-84): Obtained from N-90 as a white solid (0.05 g, 1 1 % from the chloropyrazine) after a chromatography over silica gel (cyclohexane ethyl acetate 6/1) and recrystallization from n- heptane. Ή NMR (CDC13): 7.91 (m, 2H), 7.85 (s, IH), 7.48 (m, 2H), 7.42 - 7.24 (m, 4H), 6.92 (m, I H), 6.03 (d, 1 H, J = 3.1), 5.92 (m, I H), 4.62 (s, 2H), 4.19 (s, 2H), 2.63 (q, 211, J = 7.5), 2.36 (s, 3H), 2.63 (t, 3H, J = 7.5). HRMS: calcd for C28H25FN3O3, 470.1880; found, 470.1888. 2-Benzyl-8-(4-fluorobenzyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate (P-85): Obtained from N-93 as a solid (0.04 g, 33 %) after a recrystallization in two crops from H-heptane. Ή NMR (CDCI3): 7.90 (m, 2H), 7.81 (s, 1 H), 7.58 (m, 2H), 7.47 (m, 2H), 7.40 (m, 1H), 7.36■■·- 7.24 (m, 5H), 7.00 (m, 2H), 4.60 (s, 2H), 4.21 (s, 2H), 2.19 (s, 3H). HRMS: calcd for C28H23FN3O2, 452.1774; found, 452.1789.
2-Benzyl-8-(2-methoxybenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate (P-86): Obtained from N-94 as a white solid (0.06 g, 70 %) after a recrystallization from n-heptane. !H NMR (CDCI3): 7.84 (m, 2H), 7.82 (s, 1H), 7.43-7.23 (m, 10H), 6.93 (m, 2H), 4.69 (s, 2H), 4.22 (s, 2H), 3.83 (s, 3H), 2.18 (s, 3H). HRMS: calcd for C29H26N3O3: 464.1974; found, 464.1983. 2-benzyl-6-phenyl-8-((tetrahydrofuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-87): Obtained from N-42' as a solid (0.03 g, 30 %) after a recrystallization from H-heptane and a small amount of toluene. Ή NMR (CDCI3): 7.90 (m, 2H), 7.78 (s, 1H), 7.48 - 7.41 (m, 2H), 7.40 - 7.35 (rn, 1 H), 7.34 - 7.20 (m, 5H), 4.85 - 4.75 (m, 1 H), 4.18 (s, 2H), 3.98 (td, J= 7.7, 6.2 Hz, 1 H), 3.78 (td, J= 7.8, 6.1 Hz, 1 H), 3.64 (dd, J - 14.3, 7.4 Hz, IB), 3.38 (dd, J= 14.3, 6.1 Hz, 111), 2.14 (s, 3H), 2.21 - 1.74 (m, 5H). HRMS: calcd for C26H20N3O3, 428.1974; found, 428.1936.
8-Benzyl-2-(4-(benzyloxy)benzyl)-6-(4-(benzyloxy)phenyl)imidazo[l ,2-a]pyrazin-3-yl pivalate (P-88): Obtained from N-9 as a solid (1.06 g, 48 %) after a recrystallization from cyclohexane. !H NMR (CDCI3) δ 7.86 - 7.72 (rn, 2H), 7.61 (s, 1 H), 7.60 - 7.55 (m, 2H), 7.47 - 7.36 (m, 8H), 7.36 - 7.32 (m, 2H), 7.32 - 7.29 (m, 1H), 7.29 - 7.27 (m, 1 H), 7.23 - 7.19 (m, l H), 7.19 - 7.14 (m, 2H), 7.08 - 7.01 (m, 2H), 6.93 - 6.86 (m, 2H), 5.12 (s, 2H), 5.04 (s, 2H), 4.60 (s, 2H), 4.09 (s, 2H), 1.33 (s, 9H). HRMS: calcd for C45H42N3O4, 688.3175; found, 688.3148.
2,8-Dibenzyl-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl pivalate (P-89): Obtained from N-5 as a solid (0.12 g, 42 %) after two chromatography over silica gel (dichloromethane-ethanol 99: 1 ) and (cyclohexane-ethyl acetate 90: 10). Ή NMR (CDCI3): 7.93 - 7.86 (m, 2H), 7.72 (s, 1 H),
7.68 7.60 (m, 2H), 7.52 - 7.44 (m, 2H), 7.44 7.37 (m, 1H), 7.37 - 7.20 (m, 8H), 4.66 (s,
2H), 4.20 (s, 2H), 1.36 (s, 9H). HRMS: calcd for C31H29N3O2, 476.2338; found, 476.2362. Representative synthesis of 2,8-dibenzyl-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one (Q-2) directly from the a-aminoesters N-5. Under an inert atmosphere, N-5 (0.41 g, 0.93 mmol) was dissolved in ethanol (10 mL) and 37 % hydrochloric acid (0.4 mL). This was sealed and heated at 90 °C overnight. The solution was then left to crystallize at 0 °C, the resulting precipitate was filtered, rinsed with ethanol and dried under vacuum to yield compound Q-2 as an orange powder (0.1 1 g, 30 %). 'Ή NMR (DMSO-<¾): 8.68 (s(br), 1 H), 8.01 (m, 2H), 7.52 - 7.17 (m, 14H), 4.58 (s, 2H), 4.31 (s, 2H). HRMS: calcd for C26H22N3O, 392.1763; found, 392.1776.
8-benzyl-2-(furan-2-ylmethyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one (Q-l): By using similar reaction conditions, compound Q-l (aka furimazine) was obtained as a brown powder (0.03 g, 30 %). 1 H NMR (DMSO- ): 8.38 (s, 1H), 7.96 (d, 2H, J = 7.6), 7.56 (dd, 1H, J = 1.8, 0.8), 7.54 - 7.41 (m, 5H), 7.34 - 7.19 (m, 3H), 6.39 (dd, 1H, J = 3.2, 1.8), 6.17 (dd, 1 H, J = 3.2, 0.8), 4.46 (s, 2H), 4.21 (s, 2H). HRMS: C24H20N3O2, 382.1556; found: 382.1531.
Representative synthesis of poly O-acetylated derivatives P from a-aminoesters N via step q. The considered ester (0.3 mmol) and a 1/1 mixture of acetic acid and acetic anhydride (10 mL) were heated in a microwave oven at 140 °C for four and half hours. The resulting solution was concentrated to dryness and further purified as described below.
4-((3-Acetoxy-8-benzyl-6-phenylimidazo[l ,2-a]pyrazin-2-yl)methyl)phenyl acetate (P90): Obtained from N-97 as an oil containing traces of acetic acid after co-evaporation with toluene (0.26 g). Ή NMR (CDC13): 7.90 (m, 2H), 7.80 (s, III), 7.62 (m, 2H), 7.39 (m, 5H), 7.22 (m, 311), 7.04 (m, 2H), 4.63 (s, 2H), 4.21 (s, 2H, J = 10.6), 2.31 (s, 3H), 2.21 (s, 3H). HRMS: calcd for C30H2 N3O4, 492.1923; found, 492.1927.
4-(3-Acetoxy-2-(4-acetoxybenzyl)-8-benzylimidazo[l ,2-a]pyrazin-6-yl)phenyl acetate (P-91 ): Obtained from N-95 as a white powder (0.018 g, 10 %) after a recrystallization in a mixture of toluene and cyclohexane (two crops). !H NMR (CDCI3): 7.92 - 7.86 (m, 2H), 7.75 (s, lH), 7.62 - 7.55 (m, 2H), 7.33 - 7.26 (m, 4H), 7.25 - 7.14 (m, 3H), 7.05 - 6.99 (m, 2H), 4.60 (s, 2H), 4.19 (s, 2H), 2.32 (s, 3H), 2.29 (s, 3H), 2.19 (s, 311). HRMS: calcd for C32H28N3O6, 550.1978; found, 550.1995.
4-(3-Acetoxy-8-benzyl-2-((5-methylfuran-2-yl)methyl)imidazo[l,2-a]pyrazin-6-yl)phenyl acetate (P-92): Obtained as a purple powder (0.13 g, 63 %) from N-96 after a recrystallization in a mixture of dichloromethane and cyclohexane. Ή NMR (CDCI3): 7.92 (m, 2H), 7.80 (s, 1H), 7.62 (m, 2H), 7.33 - 7.17 (m, 7H), 6.02 (d, 1H, J = 2.9), 5.91 (m, 1H), 4.62 (s, 2H), 4.18 (s, 2H), 2.35 (s, 3H), 2.34 (s, 3H), 2.27 (s, 3H). HRMS: calcd for C29H26N3O5, 496.1873; found, 496.1859.
Preparation of 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3- yl pivalate (P-93): The bisbenzylated O-pivaloate P-88 (0.18 g, 0.26 mmol) and 10 % palladium over charcoal (0.25 mg, 0.026 mmol) were suspended in ethanol (15 mL). This was stirred under one atmosphere of hydrogen for 24 hours, filtered. The filtrate was concentrated to dryness and purified by a chromatography over silica gel (dichloromethane - ethanol 97/3) to give deprotected compound as a red solid (0.06 g, 45 %). Ή NMR (DMSO- ): 9.65 (s, 1 H), 9.18 (s, 1 H), 8.10 (s, 1H), 7.87 - 7.80 (m, 2H), 7.51 - 7.43 (m, 2H), 7.34 - 7.24 (m, 2H), 7.24 - 7.15 (m, I H), 7.07 - 6.99 (in, 2H), 6.93 - 6.83 (m, 2H), 6.77 - 6.64 (m, 2H), 4.47 (s, 2H), 3.92 (s, 2H), 1.36 (s, 9H). HRMS: calcd for C31 H30N3O4, 508.2236; found, 508.2269. General protocol for the cyclization and mono-acetylation of Oil-bearing a-aminoacids O. This reaction makes good use of the difference of reactivity of the acid function and of the resulting hydroxyimidazole in comparison with the phenolic function(s) present elsewhere in these starting materials. Moreover, the variable amount of water which is present has led us to systematically proceed by the step-wise addition of initially two equivalents of acetic anhydride followed by monitoring the reaction by Ή NMR analysis of a sample before adding more acetic anhydride or working up the reaction. All this said, here is a "typical" procedure. The considered deprotected acid (0.4 mmol) was dispersed in either ethyl acetate or toluene. To this was added an initial acetic anhydride (95 microL, 0.9 mmol) and the mixture heated to reflux for 30 mn. An !H NMR analysis of sample was made and depending on the degree of dissapearance of the starting material more acetic anhydride. Care should be taken in not adding too much of this as the polyacetylation will take place quite quickly, especially in refiuxing toluene. In few, unfortunate cases, this reaction was run at room temperature in acetic anhydride and along with small amount of the target mono acetylation produc, polyacetylation was the main results. When relevant, the resulting solution was concentrated to dryness and purified as described below. The compounds obtained usually contained less than 5 % of bis acetylated material.
2,8-Dibenzyl-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-94): This compound was obtained from O-10 as a beige solid (0.1 1 g, 52 %) using toluene as a solvent and a chromatography over silica gel (dichloromethane - ethanol 98/2). !H NMR (DMSO-i¾): 9.64 (s, IH), 8.54 (s, 1H), 7.89 (m, 2H), 7.48 (m, 2H), 7.26 (m, 8H), 6.87 (m, 2H), 4.46 (s, 2H), 4.08 (s, 2H), 2.37 (s, 3H). HRMS: calcd for C^H^N^: 450.1818; found, 450.1825.
8-Benzyl-6-(4-hydroxyphenyl)-2-(3-propylbenzyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P-95): The reaction was run, using O-l 1 , in acetic anhydride at room temperature for 30 mn. The resulting solution was dispersed in water, extracted with ethyl acetate, the organic layer was washed with water, brine, dried over magnesium sulfate and concentrated to dryness. A chromatography of the residue over silica gel (cyclohexane -· ethyl acetate 4/1) gave the target compound as a beige solid (0.02 g, 10 %) along with the corresponding diacetylated product (0.13 g, 65 %). Ή NMR (CDC¾): 7.76 (m, 2H), 7.70 (s, I H), 7.61 (m, 2H), 7.30 (m, 2H), 7.22 (m, 2H), 7.07 (m, 3H), 6.90 (m, 2H), 4.62 (s, 2H), 4.19 (s, 2H), 2.56 (m, 2H), 2.16 (s, 3H), 1.65 (m, 2H), 0.96 (t, J = 7.3 Hz, 3H). HRMS: calcd for C31H30N3O3: 492.2287; found, 492.2260.
8-Benzyl-2-(furan-2-ylmethyl)-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yl acetate (P- 97): This compound was obtained from 0-12 as a beige solid (0.33 g, 60 %) using ethyl acetate as a solvent and a chromatography over silica gel (cyclohexane - ethyl acetate 2/1). Ή NMR (DMSO- ): 9.65 (s, IH), 8.55 (s, IH), 7.90 (m, 2H), 7.55 (m, IH), 7.47 (m, 2H), 7.27 (m, 2H), 7.22 (m, IH), 6.88 (m, 2H), 6.39 (dd, 1H, J = 1.8, 3.1), 6.19 (dd, IH, J = 0.8, 3.1), 4.46 (s, 2H), 4.13 (s, 2H), 2.40 (s, 3H). HRMS: calcd for C26H22N304, 440.1610; found, 440.1603.
8-Benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-(4-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P-98): This compound was obtained from 0-13 as a beige solid (0.05 g, 22 %) using ethyl acetate as a solvent and a chromatography over silica gel (cyclohexane - ethyl acetate 3/1). Ή NMR (CDCI3): 7.75 (m, 2H), 7.74 (s, IH), 7.58 (m, 2H), 7.26 (m, 4H), 6.91 (m, 2H), 5.89 (s, IH), 4.61 (s, 2H), 4.13 (s, 2H), 2.36 (s, 3H), 2.17 (s, 3H), 1.90 (s, 3H). HRMS: calcd for C28H26N3O4: 468.1923; found, 468.1922.
8-Benzyl-2-((5-ethylfuran-2-yl)niethyl)-6-(4-hydroxyphenyl)imidazo[l ,2-a]pyrazin-3-yI acetate (P99): This compound was obtained from 0-14 as a beige solid (0.05 g, 45 %) using ethyl acetate as a solvent and a chromatography over silica gel (cyclohexane - ethyl acetate 3/1). H NMR (CDCI3): 7.76 (m, 2H), 7.74 (s, IH), 7.59 (m, 2H), 7.26 (m, 4H), 6.91 (m, 211), 6.01 (m, IH), 5.90 (m, IH), 4.61 (s, 2H), 4.19 (s, 2H), 2.61 (q, 2H, J= 7.6), 2.35 (s, 3H), 1.22 (t, 3H, J= 7.6). HRMS: calcd for 0½Η26Ν304: 468.1923; found, 468.1929.
8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (P- 100): This compound was obtained from 0-9 as a beige solid (0.10 g, 68 %) using ethyl acetate as a solvent and a chromatography over silica gel (cyclohexane - ethyl acetate 2/1). Ή NMR (DMSO- ) 9.65 (s, IH), 9.20 (s, IH), 8.53 (s, I H), 7.89 (m, 2H), 7.48 (m, 2H), 7.24 (m, 3H), 7.07 (m, 2H), 6.87 (m, 2H), 6.69 (m, 2H), 4.46 (s, 2H), 3.96 (s, 2H), 2.37 (s, 3H). HRMS: calcd for C28H24N304: 466.1767; found, 466.1758.
2,8-Dibenzyl-6-(3-f!uoro-4-hydroxyphenyl)imidazo[ l ,2-a]pyrazin-3-yi acetate (P101): This compound was obtained from 0-15 as a white solid (0.11 g, 24 %) using ethyl acetate as a solvent and a chromatography over silica gel (cyclohexane - ethyl acetate 3/1) followed by a recrystallization in toluene cyclohexane. !H NMR (DMSO-< ): 7.71 (s, IH), 7.69 (m, IH),
7.60 (m, 2H), 7.52 (m, IH), 7.34 - 7.20 (m, 8H), 7.04 (m, IH), 6.67 (s(br), IH), 4.62 (s, 2H),
4.21 (s, 2H), 2.18 (s, 3H). HRMS: calcd for C28H23FN303: 468.1723; found, 468.1725. General protocol for the generation of solutions of imidazo[l,2-a]pyrazin-3(7H)-one Q by acidic hydrolysis of imidazo [ 1 ,2-a] py razin-3-yl acetate derivatives P, The considered acetate P (1 mg) was dissolved in DMSO (0.2 mL) and then diluted by adding a solution of acidic ethanol (0.3 ml) made from the addition of 37 % hydrochloric acid (ΙΟΟμΙ) on 100 % ethanol (12 mL). This 0.5 mL solution was incubated at 50°C for 2 hours to give a stock solution which was then stored at -80°C. As depicted in figure 1, in the case of compound P- 21, the LC/MS monitoring of the hydrolysis into the corresponding 8-benzyl-2-((5-ethylfuran- 2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one (Q-12; see table below) pointed out a complete reaction in less than two hours. ota: these conditions are only adapted to the hydrolysis of the protecting group (as defined above) of the oxygen on position 2 of the imidazo[ 1 ,2-a]pyrazine ring system and not to the hydrolysis of any additional acetyl groups eventually present elsewhere on the substrate of this reaction.
Bioluminescence properties of a selection of imidazo[l,2-a]pyrazin-3(7H)-one Q generated in solution from the corresponding 0-acetylated precursors P.
Some of the synthesized substrates described above were evaluated for up to the five following kinds of applications using Oplophorns-denvcd luciferases or complementation of parts of these enzymes. If bioluminescent assays are depending on cell transfection to express luciferase chimeras, then assay methods that are based on light microscope imaging may be preferred for difficult-to-transfect cells to assess the light emission from the individual cells. In the other cases, single-tube or plate-reader luminometer, digital camera (including smartphones), light detector or photographic film integrating the light in the full volume are suitable.
1- In vitro assays of bioluminescence of luciferases or fragments from cell lysate, biologic fluids, or various grade of purified proteins in buffer solution.
Measurements are typically monitored by a multi-well plate luminometer or a luminometer reading train of drops sampled in a tubing separated by air bubbles from a liquid handler. This was in particular the main method employed for screening these substrates and assessing their bioluminescence properties.
2- In cellulo assays of bioluminescence of ceil or exosome suspension. Cells can be alive in culture media, quiescent in conditions of ATP production inhibition, in soft chemical fixation conditions or in cell permeation conditions. Luciferase reporters or fragments can be fused to proteins targeting the cell surface, the cytoplasm, organelles, the nucleus or the exosomes. Measurements are typically monitored by a multi-well plate luminometer, a luminometer reading train of drops sampled in a tubing separated by air bubbles from a liquid handler, or from a cytometer with bioluminescence setting.
3- In cellulo assays and imaging of bioluminescence of adherent or non- adherent cells, organelles, exosomes, virus, or particles using a light microscope.
Protein: luciferase fusion can be expressed by cells and imaged alive in the culture media, quiescent in conditions of ATP production inhibition, in soft chemical fixation conditions or in cell permeation conditions. Proteimluciferase fusion can be added to the media and target the cell surface, cytoplasm, organelles, nucleus, exosomes or eventually carried to target cells either by virus, host-infecting cells or agents, biologic complexes or particles.
4- In vivo assays of bioluminescence of luciferase or fragment used as reporter of proteins expressed in living animals or perfused tissues using a light microscope, light scanners, cameras or bioluminescent imaging systems.
5- Bioluminescence assay of immobilized molecules, particles, complexes, virus or cells on solid 2D (membrane, slide, bead, fibre) or 3D-support (matrix, permeable beads, fibre networks, permeable tissues) with typical application as western and northern blots for protein and DNA target detection in solution, cell lysate or biofluids but also for toxics and compound detection in soils, liquids for testing bio or chemical hazard in the environment. Detection is possible in bioluminometer, digital camera (including smartphones), scanner, imaging system or photo-sensitive film.
1-In vitro assay of bioluminescence.
The representative bioluminescence curves shown in the figure 2 provided an assessement for furimazine (Q-l ), 8-benzyl-2-(furan-2-ylmethyl)-6-(4- hydroxyphenyl)imidazo[l,2-ij]pyrazin-3(7/ )-one (Q-80), 8-(2-fluorobenzyl)-2-(furan-2- ylmethyl)-6-phenylimidazo[l,2-a]pyrazin-3(7//)-one (Q-84), and 8-(2-fluorobenzyl)-2-((5- methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7/ )-one (Q-85) was obtained using a luciferase expressed in vitro as described in the following. The nano AZ DNA template was cloned into Thermo Scientific pT7CFEl -based vector and nanoKAZ was expressed using ThermoFisher Scientific 1 -Step Human Coupled IVT Kit - DNA (ref. 88881) according to the manufacturer instructions. The resulting reaction mix was diluted 400 times in buffer (DPBS (Gibco) + 0.1% (v/v) Tween 20) and conserved at -80°C. The screening of the imidazof 1 ,2-a]pyrazin-3(7H)-ones was performed in 96 well plates using 50
Figure imgf000125_0001
of a 1/20 dilution in DPBS (Gibco) + 0.1% (v/v) Tween 20 of the nanoKAZ solution. After adding 50 pL/well of a 1/50 dilution of the stock (4.5 mM) imidazo[ 1 ,2-a]pyrazin-3(7H)-ones solution in DPBS (Gibco) + 0.1% (v/v) Tweeri 20, luminescence intensity displayed by the different luciferin analogues was measured on a Berthold Centro luminometer (Remlla luminescence counting program with an integration time of 1 second), and signal decrease over time was monitored for 2 hours in a kinetic experiment with a 3 minutes increments.
To illustrate the bioluminescence properties improvement achieved for many of the imidazo[] ,2-a]pyrazin-3(7H)-ones described here, four values are provided in Table 1 : maximum intensity (VI), signal half-life (V2), area under the curve for ten minutes (V3), and area under the curve for two hours (V4), normalized using furimazine (Q- 1 ) as a reference.
The bioluminescence properties of the tested compounds are considered to be improved when at least one of the values VI -V4 is above 1, and when none of the VI -V4 values is 0.05 or below.
Table 1, bioluminescence properties of a selection of imidazo [ 1 ,2-a ] pyrazin-3(7H)-ones
Codes Structures Chemical names (Chemdraw Ultra 14) VI V2 ¥3 V4
Q-i y(- 8-benzyl-2-(furan-2-ylmethyl)-6-
(outside
pfaenylimidazof 1 ,2-a]pyrazin-3(7H)-one 1 1 1 1 the
(furimazine)
invention)
8-benzyl-2-((5-methylfuran-2-
Q-3 yl)methyl)-6-phenylimidazo[l ,2- 2.7 0.36 2.39 1 .3 a]pyrazin-3(7H)-oiie
8-benzyl-2-((5-ethylfuran-2-yl)mcthy])-
Q-12 6-phenylimidazo[l ,2-a]pyrazin-3(7H)- 2.28 0.44 2.2 1.28 one
8-bcnzyi-2-((4,5-dimethylfuran-2-
0-16 yl)methyl)-6-phenylimidazo 1 ,2- 2.07 0.3 1.88 0.93 a]pyrazin-3(7H)-oiie
8-benzyl-6-(2-fluorophcnyl)-2-(furan-2-
Q-21 ylmethyl)imidazo[ I ,2-a]pyraztn-3(7H)- 1.39 0.65 1.33 1.08 one 8-benzy!-2-(3-metfaylbenzyl)-6-
Q-14 1.8 0.38 1.66 0.96 phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
8-benzy!-2-(3-methoxybenzyl)-6-
Q-18 1.53 0.44 1.41 0.97 phenylimidazo[ 1 ,2 -a] pyrazi n-3 ( 7 H ) -one
2,8-dibenzyl-6-(2-
Q-20 fluorophenyl)imidazo[ 1 ,2-a]pyrazin- 2 0.22 1.59 0.66
3(7H)-one
8-benzyl-6-(2,6-difluorophenyl)-2-
Q-27 (furan-2-ylmcthyI)imidazo[ 1 ,2- 1.04 0.87 1.02 0.97 a]pyrazin-3(7H)-oee
8-benzyl -6-phcny 1 -2 -((5 -
0 (trif!uorotnethyl)furan-2- -28 1.31 0.27 1.1 ! 0.62 yl)methyl)imidazo[ 1 ,2-a]pyrazin-3(7II)- one
2,8-dibenzyl-6-(2 >-
Q-29 difluorophcnyl)imidazof 1 ,2-a]pyrazin- 1 .86 0.17 1.48 0.55
3(7H)-one
8-benzyl-6-(2-fluorophcny!)-2-((5-
Q-34 methylfuran-2-yl)methyl)imidazo[ 1 ,2- 2.17 0.39 1.89 1.13 a]pyrazin-3(7H)-one
8-benzyI-2-((5-cyclopropy!furan-2-
Q-36 yl)methyl)-6-phenyIimidazo[ 1 ,2- 1.74 0.36 1.64 0.83 a]pyrazin-3(7H)-onc
8-benzyl-2-(3-fluorobenzyl)-6-
Q-41 1.49 0.33 1.28 0.74 phenylimidazof l ,2-a]pyrazin-3(7H)-one 8-benzyl-2-((5-ethylfuran-2-yl)methyl)-
Q-51 6 to 6-(2-fluorophenyl)imidazo[ 1 ,2- 2.59 0.35 2.33 1.19 a]pyrazin-3(7H)-one
8-benzyl-6-(3-fluorophcnyl)-2-((5-
Q-54 methylfuran-2-yl)methyl)imidazo[ 1 ,2- 2.73 0.3 2.32 1 .22 a]pyrazin-3(7H)-one
8-benzyl-2-(2-fluorobenzyl)-6-
Q-56 1 .84 0.24 1.51 0.7 phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
8-berizyl-2-((5-ethylthiophen-2-
0-58 yI)methyl)-6-phenylimidazo[ 1 ,2- 1.71 0.32 1.61 0.69 a]pyrazin-3(7H)-one
8-benzyl-2-((4,5-dimethylfuran-2- yl)methyl) -6-{2-
Q-61 2.13 0.28 1 .97 0.78 fluorophenyl)imidazo[ 1 ,2-a]pyrazin- 3(7H)-one
2-beiizyl-8-(2-fluorobenzyl)-6-
Q-72 1 .49 0.5 1.49 0.85 phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
2-benzyl-8-(3-fluorobenzyl)-6-
Q-73 1.12 0.92 1.19 0.92 phenylimidazof 1 ,2-ajpyrazin-3(7H)-one
Q-75
2,8-dibcnzyl-6-(4- (outside
hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin- 2.03 <0.05 0.45 0.1 1 the
3(7H)-one
invention)
Q-80
8-benzyl-2-(furan-2-yImethy])-6-(4- (outside
hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin- 3.06 0.05 0.79 0.14 the
3(7H)-one
invention)
8-(3-f1uorobenzyl)-2-(furan-2-yImethyl)-
Q-81 6-phenyiitniclazo[ 1 ,2-a]pyrazin-3(7H)- 0.82 3.25 0.92 1.31 one 8-(2-fluorobenzyl)-2-(3-methylbenzyl)-
Q-82 6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)- 1.36 0.57 1.38 0.84 one
8-(2-f!uorobenzyl)-2-(3-
Q-83 methoxybenzyl)-6-phenylimidazo[ 1 ,2- 1.46 0.71 1.49 1.12 a]pyrazin-3(7H)-one
8-(2-fluorobenzyl)-2-(furan-2-yImethyl)-
Q-84 6-phenyI i midazof 1 ,2-a]pyrazin-3(7H)- 0.84 2.29 0.91 1.18 one
8-(2-fluorobenzyl)-2-(( 5-methyl furan-2-
Q-85 yl)methy!)-6-phenylimidazo[l ,2- 2.3 0.79 2.4 1.81 a]pyrazin-3(7H)-one
Q-86
8-benzyI-2-(4-hydroxybenzyl)-6-(4-
(outside
hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin- 0.09 0.64 0.08 0.07 the
3(7H)-onc (coelenterazine)
invention)
2-((5-ethylfuran-2-yl)methyl)-8-(2-
Q-96 fluorobcnzyl)-6-phenylimidazo[ 1 ,2- 2.51 0.91 2.68 1.98 a]pyrazin-3(7H)-onc
8-(3-fluorobenzyl)-2-{(5-methylfuran-2-
Q-97 yl)methyl)-6-phenylimidazo[l ,2- 2.04 1,27 2.20 2.04 a]pyrazin-3(7H)-one
8-(2-chlorobenzyl)-2-((5-methylfuran-2-
Q-98 yl)methyl)-6-phenylimidazo[ 1 ,2- 1.18 1.27 1.28 1.20 a]pyrazin-3(7H)-one
2-<(5-ethylfuran-2-yI)methyl)-8-(3-
Q-99 fluorobenzyl)-6-phcnylimidazo[ 1 ,2- 1.99 1 .36 2.13 2.08 a]pyrazin-3(7H)-one
8-(3-fluorobenzyl)-2-(3-methylbenzy!)-
Q-100 6-phenylimidazo[l ,2-a]pyrazin-3(7H)- 1.25 0.73 1.33 1.04 ne By following the hydrolysis protocol described above, the following imidazo[ l ,2- a]pyrazin-3(7H)-ones luciferins Q were made in situ from the corresponding O-protected imidazo[ 1 ,2-a]pyrazines P and evaluated for their bioluminescence properties (maximum intensity (VI), signal half-life (V2), area under the curve for 2 hours (V3), and area under the curve for ten minutes (V4), normalized using furimazine (Q-l) as a reference):
Chemical names (Chemdraw Ultra
Codes Structures VI V2 V3 V4
14)
Q-i 8-bcnzyl-2-(furan-2-ylmethyI)-6-
(outside the phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-onc 1 1 1 1 invention) (furimazine)
8-(3-fluorobenzyl)-6-(2- fluorophenyl)-2-((5-methylfuran-2-
Q-105 2.04 1.27 2.01 1.72 yl)methyl)imidazo[ 1 ,2-ajpyrazin- 3(7H)-one
8-(2,3-difluorobenzyl)-2-(furan-2-
Q-107 ylmethyl)-6-phenylimidazo[ 1 ,2- 1 ,03 1.63 1.04 1.27
0 - . ' ajpyrazin-3 (7H)-one
8-(2,3-dinuorobenzyl)-2-((5- methylfuran-2-yl)methyl)-6-
Q-108 3.15 0.53 3.09 2.1 phenylimidazo[ 1 ,2-a]pyrazin-3(7H)- one
2-benzyl-8-(2,3-difluorobenzyl)-6-
Q-117 phenylimidazo[l,2-a]pyrazin-3(7H)- 2.31 0.18 1.94 0.7 one
8-(2,6-difluorobenzyl)-2-((5- methy 1 furan-2 -y 1 )methy l)-6-
Q-121 1.41 0.94 1.43 1.37 phenylimidazo[ 1 ,2-a]pyrazin-3(7H)- one
Figure imgf000131_0001
This kind of bioluminescence assay of luciferases in solution is typically used in high throughput screening. These assays aim at measuring the intensity of light emission at single or multiple time points or to assess a process kinetic. Protocols can be adapted to several concentrations of luciferase-tagged proteins and several concentrations of substrates. These measurements do require a high intensity along with a maximum of linearity of the intensity with the substrate concentration for a large dynamic range, stable intensity, ideally for one hour as well as a low background noise. Protein :nanoKAZ chimeras can be expressed from transfected cells, Transfection can be performed using chemical agent, electroporation, or molecular moieties providing cell-absorption properties to linear or circular plasmid or lentiviral vectors. Assays can be performed on transiently transfected cells or after gene integration on constitutively nanoKAZ chimera expressing gene.
As depicted in figure 3, the dynamic range of nanoKAZ was assessed using luciferin Q-3. The enzyme was diluted three by three times and the bioluminescence has been measured on a constant volume (100 μΐ,) and at constant concentration of the substrate. The bioluminescence is linear with the enzyme concentration over 5 orders of magnitude, overwhelming the detection system. This range can be increase by two orders of magnitude by decreasing 2 times the volume for high enzyme concentration and by increasing two times the volume for low enzyme concentration.
Considering the hypothesis of a Michaelis-Menten mechanism for the nanoKAZ, the
Km and Vmax were extrapolated from the Lineweaver-Burk representation of the enzyme activity catalyzing either Q-l or Q-3. The figure 4 shows the inverse of the reaction rate versus the inverse of the substrate concentration keeping constant the concentration of the enzyme. The data have been fitted with linear regression with satisfying high correlation factors (R). The Km are the same for both substrates (Km = 2 μΜ) whereas the Vmax is 3-times lower for Q-l (0.6· 106 RLU/sec) than for Q-3 (2.0· 106 RLU/sec)..
This assessment of the protein-luciferase activity can be applied to assay from transiently transfected cells:
substrate comparison: intensity, stability, catalytic activity, Michaelis constant, catalytic mechanism, inhibition by substrate excess, inhibition by products, inactivation by sub-products;
optimization of reaction buffer;
mutation effects on luciferase catalytic activity; effect of protein-luciferase linker size, stiffness, composition on luciferase catalytic activity;
plasmid expression efficiency;
transfection efficiency;
regulation of transcription using a regulator domain upstream the luciferase gene in the plasmid;
assays of protein interactions from the complementation of two fragments of the nanoKAZ used as reporter fused to each protein partner and recovering the luciferase activity upon the association of protein partners.
This assessment of the protein-luciferase activity can be applied to assay from constitutively transfected cells:
effect of media composition, temperature, gas;
drug screening of specific gene expression (regulation of factor binding upstream the nanoKAZ gene);
effect of gene knock-in, knock-down, or knock-out by RNA interference or gene editing on protein expression, cell growth.
2- In cellulo assays of bioluminescence of cell or exosome suspension.
This kind of assays stems from the necessity of performing bioluminescent assessment on cells. Cells can be alive in growth condition using culture media, or in quiescent in conditions using inhibitor of ATP synthesis as sodium azide or deoxyglucose as the nanoKAZ reaction does not require triphosphate nucleoside. Cells can be killed after a light chemical fixation conditions by addition of paraformaldehyde (0.5%, 20 min at room temperature) then several washing step with PBS. Cell permeation conditions can be also used to keep the cell overall structure with saponin, digitonin or low concentration of Triton XI 00 in order to get soluble proteins out of cells (eventually the unbound protein-nanoKAZ chimera) or to get proteins in (as eventually the protein-nanoKAZ chimera) for targeting bound cells. Luciferase reporters or fragments can be fused to proteins targeting the cell surface, the cytoplasm, organelles, the nucleus or the exosomes.
As described for in vitro, these in cellulo assays aim at measuring the intensity of light emission at single or multiple time points or along kinetics. Protocols can be adapted to several concentrations of luciferase-tagged proteins and several concentrations of substrates. These measurements require high intensity along with a maximum of linearity of the intensity with the substrate concentration for a large dynamic range, stable intensity along measurements in a one-hour scale, and low noise. Protein-nanoKAZ chimeras can be added in the media to cells, expressed from some specific cells added to a different kind of cells or from overall transfected cells. Transfection can be performed using chemical agent, electroporation, or molecular moieties providing cell-absorption properties to linear or circular plasmid or lentiviral vectors. Assays can be performed on transiently transfected cells or after gene integration on constitutively nanoKAZ chimera expressing gene.
Measurements are typically monitored by a multi-well plate luminometer, a luminometer reading train of drops sampled in a tubing separated by air bubbles from a liquid handler. As opposite to in vitro assay, in cellulo assay can be performed using a cytometer with bioluminescence setting.
Four kinds of experiments are performed as cell-based bioluminescent assay:
2.1 Protein-nanoKAZ binding assay at the untouched cell surface or inside permeabilized cells: the protein-nanoKAZ chimeras can be free or associated to a complex, a bead, a virus, another cell. These cell-based assays are typically developed for hormone and cytokine binding diagnostics, cell targeting diagnostics, cell infectibility diagnostic, drug screening for host cell protection against infections;
2.2 Measurement of the luciferase bioluminescence intensity from individual cell expression of the protein-nanoKAZ chimera from transiently or constitutively transfected cells or chimera import into the cell with auto-import domain fusion, electroporation, or injection. Typically assay development for regulation of gene expression. Screening of drugs, RNAi, pathogens and environmental factors on specific gene regulation;
2.3 Measurement of the luciferase bioluminescence intensity to assay the number of protein-naKAZ ( -end 1-85 fragment) and protein-noKAZ (C-end 86-166 fragment) associated providing luciferase activity recovery by complementation for transfected cells as described for in vitro assays. Typically assay development for protein- protein interaction inside or at the surface of cells and assay of alteration by drugs, RNAi, pathogens and environmental factors;
2.4 Measurement of the luciferase bioluminescence intensity to assay the number of complementation obtained from the addition of protein-nanoKAZ fragment chimeras free or associated to a complex, a bead, a virus, another cell and added to intact cells providing a complementary protein-nanoKAZ fragment chimeras at their surface or inside the permeabilized cells. Identical applications to 2.1 but in which the light is provided by the complementation of nanoKAZ fragments instead of the presence of the foil fusion. The stringency of the system reduces the risk of non-specific interactions.
3- In cellulo assays and imaging of bioluminescence of adherent or non- adherent cells, organelles, exosotnes, virus, or particles using a light microscope.
The acquisition of bioluminescence using a light microscope provides a quantitative measurement of light emission cell by cell, the main advantage. This is meaningful especially when the transfection efficiency is low in the case of nanoKAZ expression provided by plasmids. While nanoKAZ is active and does not require ATP, cells can be alive in culture media, quiescent in conditions of ATP production inhibition (sodium azide, deoxyglucose), in light chemical fixation conditions (0.5% PFA 20 min at RT then 2 PBS wash), in cell permeation conditions (digitonine, saponine, triton). The higher is the bioluminescence efficiency, the lower is the exposure time and the lower is the sensitivity required for the detector or camera. NanoKAZ with developed substrates such as Q-3 or Q-12 opens the bioluminescence imaging to standard light microscope. The lower the exposure time, the higher the resolution, which is impeded by the motion of living cells. Any gain in light emission enhances the quality of images. Luciferase reporters or fragments can be fused to proteins targeting the cell surface, cytoplasm, organelles, nucleus or exosomes and can be carried by virus, host-infecting cells or agents, biologic complexes, beads or any particles. The second advantage of microscopy is to provide the location of the light emission with a subcellular resolution.
For exposure time from seconds to minutes, the bioluminescence in cells was imaged using a cooled EMCCD camera (Andor) on a wide-field inverted microscope (Axiovert 200, Zeiss) using an air-objective 20 x 0.75 NA and an apochromat water-objective 63 x 1.2 NA in a light-tight black box. All light sources came from out of the box through controlled shutters. The microscope has a motorized platine for slides or plate, allowing automated well-plate screening. For shorter exposure time (less than one second), a highly sensitive avalanche photo-diode (PhoteklF) was used as detector in the same microscope system aside the camera. This highly sensitive detector allows screening large number of cells with sub-second exposure times but does give neither subcellular details, organization nor location of bioluminescent moieties but only light on the full cell. The advantages compared to in vitro measurements acquired using luminometers is to restrict the measurement of the photon emission intensity to a single cell in growth conditions and allows fast kinetics. With the enzyme/substrate pair, nanoKAZ analogue Q-3, the time acquisition for a 96-well plate is in one-hour range when using the standard system with an EMCCD camera, and in one-minute range when using the avalanche photo diode detector. The experience times were extended 3 folds with rianoKAZ/fiirimazine to get the same quality as with nano AZ/analogue Q-3. Any increase of the catalytic efficiency and photon emission improves the resolution of images and decreases the duration of the experience.
NanoKAZ fragment complementation/ analogue Q-3 was used as bioluminescent reporter for protein interaction as described above (2.3 and 2.4). HE and Jurkat cells were cotransfected with two plasmids encoding separately protein partners tagged with either naKAZ or noKAZ as described above. The plasmid pair ratio and the total amounts of plasmids were optimized to get the highest transfection and protein pair expression; various examples are shown in figure 5.
4- In vivo assays of bioluminescence of luciferase or fragment used as reporter of proteins expressed In living animals or perfused tissues using light microscope, light scanners, cameras, or bioluminescent imaging systems.
Furimazine (Q-l) and derivatives have emission spectra centred on 460 nm but present large peaks bleeding beyond 580 nm. The bioluminescence obtained with the analogue Q-3 provides photons with 10% of them having a wavelength beyond 560 nm up to 600 nm. This wide spectra emission toward red is thus allowing the use of the nanoKAZ/furimazine for in vivo imaging. The light emission beyond 580 is thousands times higher than red emitting firefly or beetle luciferases. IL7:nanoKAZ and analogue Q-3 were injected in mice.
Quantitative images of high quality were acquired with bioluminescence imaging system
( s). 5- Bioluminescence assay of immobilized molecules, particles, complexes, virus or cells on solid surface or 3D-support
The typical applications are western and northern blots for protein and DNA target detection from solution, cell lysate or biofluids. These compounds are immobilized on 2D surfaces (membrane, slide, bead, fibre) or 3D support (matrix, gel, permeable bead, fiber networks, permeable tissues). Protein-nano AZ can be used to reveal the presence of an interacting target as antibodies:nanoKAZ before binding or after binding depending of the alteration of the nano AZ activity during the immobilization. These applications could be extended to toxics and compound detection in soils, liquids for testing bio or chemical hazard in the environment when those molecules can be immobilized on the support. Applications could be developed in paper-based microfluidic point of care diagnostic devices using bioluminescence detection {Lab. Chips 2013, 13, 2210-2251). Detection is possible using a bioluminometer, digital camera (including smartphones), seamier, imaging system or photosensitive film.
Long term stability property of the O-acetylated luciferins precursors P/(I)
Two 1H NMR spectra of the same batch of compound 8-benzyl-2-((5-methylfuran-2- yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate (stored at 4 °C) were made on the day of its production (figure 6 A) and 30 months after (figure 6B) respectively.
The comparison of the two !H NMR spectra underlines the advantageous stability of the O-acetylated luciferins precursors with the general formula I.
Similar results were obtained with O-acetylated luciferins precursors stored at room temperature.
The above results are in sharp contrast with the precautions required to maintain the quality of commercially available luciferins such as furimazine or coelenterazine, underlying their very weak storage stability. Indeed, it is recommended to store these luciferins, dessicated or in solution, at -20°C, or for longer storage at -70°C, protected from light, as free of oxygen as possible, being specified that said luciferins may be stored at 4°C for up to two weeks only.

Claims

1. Use of a compound of following formula (I) in presence of a solution comprising a strong acid to detect, and/or measure the enzymatic activity of, a luminogenic protein, in vitro, in cellulo or ex vivo;
Figure imgf000138_0001
wherein :
Ri represents H or a group selected from Ci-Cg alkyl, C3-C7 cycloalkyl, C6-Ci0 aryl, aralkyl and C5-Cio-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, C6-CIQ aryl, aralkyl and CVCio-membered heteroaryl groups being optionally substituted by at least one Y| group;
R2 represents a group selected from C Cio aryl and Cs-Cio-membered heteroaryl groups, said Cg-Cio aryl and C5-CiQ-membered heteroaryl groups being optionally substituted by at least one Y2 group;
or Ri and R2 together form with the two carbon atom to which they are respectively attached a C5-C7 cycloalkene group, a C4-C7 heterocycloalkene group, or a C C10 arene, said C5-C7 cycloalkene group and C4-C7 heterocycloalkene groups being fused with a C6-C)o arene, said C5-C7 cycloalkene group, C4-C7 heterocycloalkene group, C6-C;o arene, C5-C7 cycloalkene group and C4-C7 heterocycloalkene groups being optionally substituted by at least one Y] group;
R3 represents H, a Ci-C6 alkyl, an aralkyl group, a hetaralkyl group or a heterocycloalkyl-CH2- group, said Ci-C6 alkyl, aralkyl group, hetaralkyl group and heterocycloalkyl-CH2- group being optionally substituted by at least one Y3 group;
R4 represents H or a group selected from Ci-C6 alkyl and C3-C7 cycloalkyl groups, said Q-Ce alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
R5 represents a
Figure imgf000138_0002
group and - C(=0)ORa group being optionally substituted by at least one Y5 group; Ra represents H, a C]-C6 alkyl group, a C3-C7 cycloalkyl group, a Ce-Cio aryl, or an aralkyl group, said C]-C6 alkyl group, C3-C7 cycloalkyl group, C6-Cio aryl, and aralkyl group being optionally substituted by at least one Ya group;
Z represents a Ce-Cio aryl, a C5-C1 o-membered heteroaryl groups, a Ci-C6 alkyl, a C3- C7 cycloalkyl, a C4-C7 heterocycloalkyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetrahydrofuranyl and dioxolanyl, said C6-C'l 0 aryl, C5-C 1 o-membered heteroaryl groups, Ci- C6 alkyl, C3-C7 cycloalkyl and C4-C7 heterocycloalkyl being optionally substituted by at least one Yz group;
said Yi, Y2, Y12, Y3, Y4, Y5, Ya and Yz groups being each independently selected from:
a C C6 alkyl;
a C3-C7 cycloalkyl;
a C6-Cio aryl;
a C5-C1 o-membered heteroaryl group;
an halogen, in particular -F;
a CF3 group;
a -CN group;
a - ORj group;
a - OSO3H group;
a -NRjRjj group;
a guanidinyl group;
a -C(=0)ORa group, Ra being as defined above;
¾ and ¾ each independently represent II, a Ci-C6 alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C4-C7 heterocycloalkyl group.
2. Use according to claim 1 , wherein:
- Ri represents H;.
- R4 represents H;
- R5 represents (C=0)Me;
- R3 represents an aralkyl group or a hetaralkyl group, optionaly substituted as defined in claim 1, in particular a benzyl, optionally substituted as defined in claim 1, more particularly by at least one halogen, even more particularly F;
- R2 represents a phenyl, optionally substituted as defined in claim 1, R2 representing in particular a phenyl optionally substituted by at least one group Y2 selected from:
a Ci-Ce alkyl, in particular a methyl;
an halogen, in particular F;
a -OR; group;
¾ representing H, a Ci-Cg alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective groups, ¾ representing in particular H, Me or Bn;
R2 bein more particularly selected from the group consisting of:
Figure imgf000140_0001
and/or
- Z is selected from phenyl, furanyl, thiophenyl, and is optionally substituted as defined in claim 1 ,
Z representing in particular a phenyl, furanyl, or thiophenyl, optionally substituted by at least one Y/_ group selected from:
a C C6 alkyl;
a C3-C7 cycloalkyl;
an halogen, in particular F or CI;
a -CF3 group;
a OR; group, in particular a -O e group;
Z being more particularly selected from the group consisting of:
Figure imgf000141_0001
3. Use according to anyone of claims 1 to 2, wherein the luminogenic protein is a luciferase derived from Oplophorus luciferase, more particularly the nanoKAZ luciferase.
4. Compound of formula (I) as defined in anyone of claims 1 to 2 for use, after contacting with a solution comprising a strong acid, in a method in vivo of detecting, and/or measuring the enzymatic activity of, a luminogenic protein.
5. Kit comprising :
i) A compound of formula (I) as defined in anyone of claims 1 to 2 ; and ii) A solution comprising a strong acid;
and optionally further comprising a compound selected from the group consisting of a luminogenic protein, a polynucleotide encoding the luminogenic protein, a recombinant vector comprising the polynucleotide, and a transformant comprising the polynucleotide, said luminogenic protein being in particular an Oplophorus luciferase, more particularly the nanoKAZ luciferase.
6. Compound of following formula (II) :
Figure imgf000141_0002
Ri represents H or a group selected from Ci-C6 alkyl, C3-C7 cycloalkyl, C6-Ci0 aryl, aralkyl and Cs-Cio-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, C6-Cio aryl, aralkyl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Yi group;
R? represents a group selected from C6-Cio aryl and Cs-Cio-membered heteroaryl groups, said Cg-Cio aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
or R| and R2 together form with the two carbon atom to which they are respectively attached a C5-C7 cycloalkene group, a C4-C7 heterocycloalkene group, or a C6-Cio arene, said C5-C7 cycloaikene group and C4-C7 heterocycloalkene groups being fused with a C6-CIQ arene, said C5-C7 cycloaikene group, C4-C7 heterocycloalkene group, Ce-Cio arene, C5-C7 cycloaikene group and C4-C7 heterocycloalkene groups being optionally substituted by at least one Yn group;
R3 represents H, a Ci-C6 alkyl, an aralkyl group, a hetaralkyl group or a heterocycloalkyl -CH2- group, said Ci-C6 alkyl, aralkyl group, hetaralkyl group and heteracycloalkyl-C¾- group being optionally substituted by at least one Y3 group;
R4 represents H or a group selected from Ci -C6 alkyl and C3-C7 cycloalkyl groups, said Ci-Ce alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
R5 represents a - C(=0)Ra group or a -C(=0)ORa group, said C(=0)Ra group and - C(=0)ORa group being optionally substituted by at least one Y5 group;
Ra represents H, a Ci-C6 alkyl group, a C3-C7 cycloalkyl group, a C6-Cio aryl, or an aralkyl group, said Ci-C6 alkyl group, C3-C7 cycloalkyl group, C C;o aryl, and aralkyl group being optionally substituted by at least one Ya group;
Z represents a QJ-CIQ aryl, a Cs-Cio-membered heteroaryl groups, a C|-C6 alkyl, a C3- C? cycloalkyl, a C4-C7 heterocycloalkyl, Z being in particular selected from phenyl, furanyl, thiophenyl, pyridinyl, imidazolyl, oxazolyl, oxadiazolyl, cyclopentyl, cyclohexyl, tetrahydrofuranyl and dioxolanyl, said Cg-Cjo aryl, Cs-Cio-membered heteroaryl groups, C C6 alkyl, C3-C7 cycloalkyl and C4-C7 heterocycloalkyl being optionally substituted by at least one Yz group;
said Yi, Y2, Y12, Y3, Y4, Y5, a and Yz groups being each independently selected from:
a CrC6 alkyl;
a C3-C7 cycloalkyl;
a C6-Cio aryl;
a Cs-Cio-membered heteroaryl group;
an halogen, in particular -F;
a -CF3 group;
a -CN group;
a -ORi group;
a - -OSO3H group;
a -NRjRii group;
a guanidinyl group; a -C(=0)ORa group, Ra being as defined above;
Ri and ¾ each independently represent H, a Ci-C6 alkyl group, a C3-C10 cycloalkyl group, an aralkyl group or an ad hoc protective group; or together form with the nitrogen atom to which they are attached a C4-C7 heterocycloalkyl group;
with the proviso that when Ri and R4 represent H, then :
R2 is different from unsubstituted phenyl, and 4-hydroxy-phenyl optionally protected by an ad hoc protective group, or
R3 is different from unsubstituted benzyl, or
Z is different from unsubstituted phenyl, 4-hydroxy-phenyl, optionally protected by an ad hoc protective group, and from unsubstituted furan.
7. Compound of following formula (III):
Figure imgf000143_0001
wherein :
Ri represents H or a group selected from Q-Ce alkyl, C3-C7 cycloalkyl, Ce-Cio aryl, aralkyl and Cs-Cio-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, Ce-Cio aryl, aralkyl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Yi group;
R2 represents a group selected from C6-Cio aryl and Cs-Cio-membered heteroaryl groups, said C6-Ci0 aryl and Cs-Cio-membered heteroaryl groups being optionally substituted by at least one Y2 group;
R3 represents an aralkyl group, said aralkyl group being optionally substituted by at least one Y3 group;
R4 represents H or a group selected from Ci-C6 alkyl and C3-C7 cycloalkyl groups, said Ci-C6 alkyl and C3-C7 cycloalkyl groups being optionally substituted by at least one Y4 group;
Z represents a group of following formula :
(Z2) ; or an oxazolyl, or an oxadiazolyl; R, R', R" and R' " each independently represent H or a group selected from:
a C]-C6 alkyl group;
a C3-C7 cycloalkyl group;
an halogen;
a -OR; group;
a CF3 group;
X represents O or S;
said Yi , Y2, Y3 and Y4, groups being each independently selected from:
a Ci-C6 alkyl group;
- a C3-C7 cycloalkyl group;
an halogen;
a -OR;i group;
R; and R;, each independently represent H, a Cj-Cg alkyl group, a C3-C10 cycloalkyl group or an aralkyl group;
provided that:
when Z~Z1 :
o at least one of R, R' and R" does not represent H; or
o when R, R' and R" represent H, then R2 represents a group selected from C6- C10 aryl and CVCio-membered heteroaryl groups substituted by at least an halogen group, in particular --F, R2 representing in particular a 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from Q-Cit) aralkyl groups substituted by at least an halogen group, in particular - F;
when Z=Z2,:
o at least one of R, R', R" and R"" does not represent H; or
o when R, R', R" and R' " represent H; then R2 represents a group selected from
C0-C10 aryl and C5-C 1 o-mcmbercd heteroaryl groups substituted by at least a halogen group, in particular -F, R? representing in particular 2-F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from C6-Cio aralkyl groups substituted by at least an halogen group, in particular -F; or
o when R, R' and R' " represent H and R" represents a Cj-Cg alkyl group or a -
OR; group; then R2 does not represent a 4-HO-Ph- group, R2 representing in particular a phenyl group or a group selected from Cg-Cjo aryl and Cs-Cio-membered heteroaryl groups substituted by at least a halogen group, in particular -F, R2 representing more particularly a 2- F-phenyl, a 3-F-phenyl, or a 2,6-diF-phenyl group; or R3 represents a group selected from C6- Cio aralkyl groups substituted by at least an halogen group, in particular F.
8. Compound according to claim 7, of following formula (Ilia):
Figure imgf000145_0001
wherein:
R.2 being in particular selected from the group consisting of:
Figure imgf000145_0002
R3 being in particular selected from the group consisting of:
Figure imgf000145_0003
Z is selected from the group consisting of:
Figure imgf000145_0004
Compound of following formula (IV): wherein :
R; , R2, R3) R4 and Z are as defined in claim 7,
R5 is as defined in claim 1 ,
and of following formula (V):
Figure imgf000146_0001
wherein Rj , R2, R , R4 and Z are as defined in claim 7,
and of following formula (VI):
Figure imgf000146_0002
wherein :
R] , R?, R3, R4 and Z are as defined in claim 7;
Rd represents a group selected from C)-C6 alkyl, C3-C7 cycloalkyl and aralkyl, particular methyl, ethyl, or benzyl
10. Compound according to anyone of claim 7 or 9, wherein:
- R| represents H;
- R4 represents H;
- R represents a benzyl group, optionaly substituted as defined in claim 7, particular by at least one halogen, more particularly F,
R3 being more particulary selected from the group consisting of: - R.2 represents a phenyl, optionally substituted as defined in claim 7, R.2 representing in particular a phenyl optionally substituted by at least an halogen, in particular F;
R being more particularly selected from the group consisting of:
Figure imgf000147_0001
and/or
- Z is selected from Zl and Z2,
R, R', R" and R'" each independently representing in particular H or a group selected from:
a Ci -C6 alkyl group;
a C3-C7 cycloalkyl group;
an halogen;
a -O ; group;
a - CF3 group;
Z being more particularly selected from the group consisting of:
11. Compound :
according to claim 7, chosen from the compounds having the following names: 8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]p>Tazin-3(7H)-one
8-benzyl-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one 8-benzyl-6-(2-iluorophenyl)-2-(furan-2-ylmethyl)imidazo[l ,2-a]pyrazin-3(7H)-one
8-benzyl-2-(3-methylbenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
8-benzyl-2-(3-methoxybenzyl)-6-phenylimidazo[l ,2-a]p Tazin-3(7H)-one
2,8-dibenzyl-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3(7H)-one
8-benzyl-6-(2,6-difluorophenyl)-2-(l\iran-2-ylmethyl)imidazo[l ,2-a]pyrazin-3(7H)-one
8-benzyl-6-phenyl-2-((5-(trifluoromethyl){uran-2-yl)methyl)imidazo[ l ,2-a]pyrazin-3(7H)-one 2,8-dibenzyl-6-(2,6-diiluorophenyl)imidazo[l ,2-a]pyrazin-3(7H)-one
8-benzyl-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3(7H)-one 8-benzyl-2-((5-cyclopropylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one 8-benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
8-benzyl-2-((5-ethy]furan-2-yl)methyl)-6-(2-fluorophenyl)imidazo[l ,2-a]pyrazin-3(7II)-one 8-benzyl-6-(3-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3(7H)-one 8-benzyl-2-(2-fluorobenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
8-benzyl-2-((5-ethylthiophen-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one 8-benzyl-2-((4,5-dimethyli iran-2-yl)methy))-6-(2-nuorophcnyl)imidazo[ 1 ,2-a]pyrazin-3(7H)- one
2-benzyl-8-(2-iluorobenzyl)-6-phenylimidazo[ l ,2-a]p Tazin-3(7H)-one
2-benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[l ,2-a]p>Tazin-3(7H)-one
8-(3-fluorobenzyl)-2-(fliran-2-ylmethyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
8-(2-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one
8-(2-f!uorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[l,2-a]pyrazin-3(7H)-one
8-(2-fluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[l ,2-a]p>Tazin-3(7H)-one
8-(2-fluorobenzyl)-2-((5-methy]iuran-2-yl)methyl)-6-phenylimidazo[ l,2-a]pyrazin-3(7H)-one 8-(3-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one
8-(3-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3(7H)-one
2-((5-ethyliuran-2-yl)rnethyl)-8-(3-f!uorobenzyl)-6-phenylimidazo[ l,2-a]pyrazin-3(7iI)-one 8-(2-chlorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylirnidazo[ l,2-a]pyrazin-3(7H)-one 8-(3-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H^ 2-((5-ethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7H)-one 8-(3-fluorobenzyl)-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[ l ,2-a]pyrazin- 3(7H)-one
8-(2,3-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-onc 8-(2,3-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3(7/ )- one
2-benzyl-8-(2,3-difluorobenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)-one
8-(2,6-Difluorobenzyl)-2-((5-methylftiran-2-yl)methyl)-6-phenylimidazo[l >2-a]pyrazin-
3(7H)-one
8-(2,3-Diiluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin- 3(7H)-one
8-(2,3-Difluorobenzyl)-2-((5-ethylftiran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7/ )- one
8-(2,6-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3(7H)- one
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylirnidazo[l ,2-a]pyrazin- 3(7//)-one
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[ l ,2-a]pyrazin- 3(7H)-one
8-(2,3-dilluorobenzyl)-2-((4-ethyl-5-methylfiiran-2-yl)methyl)-6-phenylimidazo[ l ,2- a]pyrazin-3(7H)-one
8-(2,3-difluorobenzyl)-2-((5-ethyl-4-methylfiiran-2-yl)methyl)-6-phenylimidazo[l ,2- a]pyrazin-3(7H)-onc
8-benzyl-2-(furan-2-ylmethyl)-6-(3-hydroxyphenyl)imidazo[ l ,2-a]pyrazin-3(7//)-one;
according to claim 9, chosen from the compounds having the following names: 8-benzyl-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((4,5-dimethyIfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-6-(2-iluorophenyl)-2-(furan-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yi acetate
8-benzyl-2-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
2,8-dibenzyl-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(2,6-difluorophenyl)-2-(furan-2-ylmethyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-bmzyl-6-phenyl-2-((5-(trifluoromethyl)furan-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate 2,8-dibenzyl-6-(2,6-difluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[ l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-cyc opr(>pyl uran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-2-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylfliran-2-yl)methyl)-6-(2-fluorophenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-benzyl-6-(3-fluorophenyl)-2-((5-methylfiaran-2-yl)methyl)imidazo[l ,2-a]pyrazin-3-yl acetate
8-bcnzyl-2-(2-iluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethylthiophen-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-y! acetate 8-benzyl-2-((4,5-dimethylfTO
acetate
2-benzyl-8-(2-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-benzyl-8-(3-fluorobenzyl)-6-phenylimidazo[ l,2-a]pyrazin-3-yl acetate
8-(3-f1uorobenzy])-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzy])-2-(3-methylbenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2-fluorobenzyl)-2-(fiiran-2-ylmethyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
8-(2-fluorobenzyl)-2-((5-methylfiiran-2-yl)m
acetate
8-(3- luorobenzyl)-2-(3-methoxybenzyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate 8-(3-fluorobenzyl)-2-(3-methylbenzyl)-6-phenylimidazo[ ! ,2-a]pyrazin-3-yl acetate
2-((5-ethylfiiran-2-yl)methyl)-8-(3-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2-chlorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-y^ acetate
8-(3-fluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]p>Tazin-3-yl acetate
2- ((5-ethylfuran-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,6-diiluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylirnidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3,5-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(3-chlorobenzyl)-2-(iuran-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(3-fluorobenzyl)-6-(2-fluorophenyl)-2-((5-methylfuran-2-yl)methyl)imidazo[l ,2-a]pyrazin-
3- yl acetate
8-(2,3-difluorobenzyl)-2-(furan-2-ylmethyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(2,3-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,5-difluorobenzyl)-2-(iuran-2-ylmethyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-(2,5-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(3,5-difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
2-benzyl-8-(2,3-difluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(2-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(3-(trifluoromethyl)benzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
2-benzyl-6-phenyl-8-(2,3 ,5-trifluorobenzyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((5-methylfuran-2-yl)methyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
8-(2,5-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3- yl acetate
8-(2,5-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,3-Difluorobenzyl)-2-((4,5-dimethyliljran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3- yl acetate
8-(3,5-Difluorobenzyl)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3- yl acetate
2-(furan-2-ylmethyl)-6-phenyl-8-(2,3,5-trifluorobenzyl)imidazo[l,2-a]pyrazin-3-yl acetate 8-(2,3-Difluorobenzyl)-2-((5-ethyliurdn-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-(3,5-Difluorobenzyl)-2-((5-ethylfiiran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-(2,6-Difluorobenzyl)-2-((5-ethylfuran-2-yl)methyl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate
8-(2,6-DittuorobenzyI)-2-((4,5-dimethylfuran-2-yl)methyl)-6-phenyliraidazo[l ,2-a]pyrazin-3- yl acetate
2-((4,5-Dimethylforan-2-yl)methyl)-8-(2-fluorobenzyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
2-((4,5-Dimethylfuran-2-yl)methyl)-8-(3-fluoroben^yl)-6-phenylimidazo[l,2-a]pyrazin-3-yl acetate 8-Benzyl-6-phenyl-2-((5-propylfuran-2-yl)methyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate (133)
8-(2,3-difluorobenzyl)-2-((4-ethyl-5-methyl&ran-2-yl)methyl)-6-phenylimidazo[l,2- a]pyrazin-3-yl acetate
8-benzyl-2-((4-ethy]-5-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate 8-benzyl-2-((5-isopropyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2-a]pyrazin-3-yl acetate
8-benzyl-2-((5-ethyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[ 1 ,2-a]pyrazin-3-yl acetate 8-(23-difluorobenzyl)-2-((5-ethyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[l ,2- a]pyrazin-3-yl acetate
8-benzyl-2-(l-(furan-2-yl)ethyl)-6-phenylimidazo[ l ,2-a]pyrazin-3-yl acetate
8-(2,3-difluorobenzyl)-2-((5-isopropyl-4-methylfuran-2-yl)methyl)-6-phenylimidazo[ i ,2- a]pyrazin-3-yl acetate
8-benzyl-2-(naran-2-ylmethyl)-6-(3-hydroxyphenyl)imidazo[ 1 ,2-a]pyrazin-3-yl acetate ; or
- according to claim 9, chosen from the compounds of the following names:
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid
2-((3-benzyl-5-phenylp Tazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoic acid
2-((3-benzyl-5-phenylp>Tazin-2-yl)amino)-3-(4,5-dirnethylfuran-2-yl)propanoic acid 2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
2-((3 -benzyl-5-phenylpyrazin-2-yl)amino)-3 -(3 -methoxyphenyl)propanoic acid
(3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)phenylalanine
2-((3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-(trifluoromethyl)furan-2-yl)propanoic acid (3-benzyl-5-(2,6-difluorophenyl)pyrazin-2-yl)phenylalanine
2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(5-methy]furan-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-cyclopropylfuran-2-yl)propanoic acid 2-((3-bcnzyl-5-phenylpyrazin-2-yl)amino)-3-(3-fluorophenyl)propanoic acid
2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(5-ethylfuran-2-yl)propanoic acid 2-((3-benzyl-5-(3-fluorophenyl)p>Tazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid 2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(2-fluorophenyl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(5-ethylthiophen-2-yl)propanoic acid
2-((3-benzyl-5-(2-fluorophenyl)pyrazin-2-yl)amino)-3-(4,5-dimethylfuran-2-yl)propanoic acid (3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)phenylalanine
(3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)phenylalanine
2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(ftiran-2-yl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoic acid 2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid
2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylftiran-2-yl)propanoic acid 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(3-methoxyphenyl)propanoic acid 2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(m-tolyl)propanoic acid
3-(5-ethyl uran-2-yl)-2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)propanoic acid 2-((3-(2-chlorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid
2- ((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-methylfuran-2-yl)propanoic acid
3- (5-ethylfuran-2-yl)-2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)amino)propanoic acid. 3-(3-(2,6-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(3,5-difluorobcnzyl)-5-phenylpyrazin-2-yl)-2-(fliran-2-ylmethyl)propanoic acid
3-(3-(3-chlorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(3-fluorobenzyl)-5-(2-fluorophenyl)pyrazin-2-yl)-2-((5-methylfiiran-2- yl)methyl)propanoic acid
3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmcthyl)propanoic acid
3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-(furan-2-ylmethyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
3-(3-(3,5-difluoroberizyl)-5-phenylpyrazin-2-yl)-2-((5-methylfuran-2-yl)methyl)propanoic acid
2-benzyl-3-(3-(2,3-difluorobcnzyl)-5-phenylpyrazin-2-yl)propanoic acid
2-benzyl-3-(5-phenyl-3-(2-(trifluoromethyl)benzyl)pyrazin-2-yl)propanoic acid
2- benzyl-3-(5-phenyl-3-(3-(trifluoromethyl)benzyl)pyrazin-2-yl)propanoic acid
2-benzyl-3-(5-phenyl-3-(2,3,5-trifluorobenzyl)pyrazin-2-yl)propanoic acid
3- (3-(2,6-difluorob(mzyl)-5-phenylpyrazin-2-yl)-2-((5-methylfxjran-2-yl)methyl)propanoic acid
3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid 3-(3-(2,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)methyl)propanoic acid 3-(3-(2,3-difluorobenzyl)-5-phenylpyTazin-2-yl)-2-((4,5-dimethyliliran-2-yl)methyl)propanoic acid
3-(3-(3,5-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid
3-(furan-2-yl)-2-((5-phenyl-3-(2,3,5-trifluorobenzyl)pyrazin-2-yl)methyl)propanoic acid 3-(3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)mcthyl)propanoic acid 3-(3-(3,5-di{luorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)methyl)propanoic acid 3-(3-(2 )-diiluorobenzyl)-5-phenylpyrazin-2-yl)-2-((5-ethylfuran-2-yl)rnethyl)propanoic acid 3-(3-(2,6-dilluorobenzyl)-5-phenylp\Tazin-2-yl)-2-((4,5-dimethylfuran-2-yl)methyl)propanoic acid
3-(4,5-dimethylfuran-2-yl)-2-((3-(2-fluorobenzyl)-5-phenylpyrazin-2-yl)methyl)propanoic acid
3-(4,5-dimethyliuran-2-yl)-2-((3-(3-fluorobenzyl)-5-phenylpyrazin-2-yI)methyl)propanoic acid
2-((3-(2,3-difluorobenzyl)-5-phenyIp xazin-2-yl)amino)-3-(4-ethyl-5-methylfuran-2- yl)propanoic acid
2-((3-benzyl-5-phenylpyrazin-2-yl)amino)-3-(4-ethyl-5-methylftiran-2-yl)propanoic acid 2-((3-benzyl-5-pheny]p Tazin-2-yl)amino)-3-(5-isopropyl-4-rnethylfuran-2-yl)propanoic acid 2-((3-benzyl-5-phenylp>Tazin-2-yl)amino)-3-(5-ethyl-4-methylfuran-2-yl)propanoic acid 2-((3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-ethyl-4-methylfuran-2- yl)propanoic acid
2-((3-benzyl-5-phenylp>iazin-2-yl)amino)-3-(furan-2-yl)butanoic acid
2-((3-(2,3-difluorobenzyl)-5-phenylpyrazin-2-yl)amino)-3-(5-isopropyl-4-methylfuran-2- yl)propanoic acid
2-((3-benzyl-5-(3-(benzyloxy)phenyl)pyi-azin-2-yl)amino)-3-(furan-2-yl)propanoic acid 2-((3-benzyl-5-(3-(hydroxy)phenyl)pyrazin-2-yl)amino)-3-(furan-2-yl)propanoic acid.
12. Compound of followin formula (VII):
Figure imgf000154_0001
wherein :
Y represents a leaving group, in particular an halogen, more particularly a -CI; Ri represents H or a group selected from Ci-C6 alkyl, C3-C7 cycloalkyl, C6-Cio aryl, aralkyl and C5-C 1 o-membered heteroaryl groups, said Ci-C6 alkyl, C3-C7 cycloalkyl, C6-Cio aryl, aralkyl and C5-C1 o-membered heteroaryl groups being optionally substituted by at least one Yi group;
R2 represents a group selected from C6-Ci0 aryl and C5-C 1 o-membered heteroaryl groups, said C6-Ci0 aryl and C5-Ci0-membered heteroaryl groups being optionally substituted by at least one Y2 group;
R3 represents an aralkyl group, said aralkyl group being optionally substituted by at least one Y group;
said Yi, Y2 and Y3 groups being each independently selected from:
a Ci-C6 alkyl group;
a C3-C7 cycloalkyl group;
an halogen;
a -OR;, group;
R„ represents II, a C|-C6 alkyl group, a C3-C10 cycloalkyl group or an aralkyl group; with the proviso that said compound is not of one of the following formulae:
Figure imgf000155_0001
13. Use of a compound of formula (III) or (Ilia) as defined in claim 7 or 8 as an in vitro or ex vivo diagnostic tool.
14. Compound of formula (III) or (Ilia) as defined in claim 7 or 8 for use as an in vivo diagnostic tool.
15. Process of preparation of a compound of formula (III) as defined in claim 7, comprising a step of conversion of a compound of formula (IV) as defined in claim 9 into a compound of formula (III), in particular by contacting said compound of formula (IV) with a strong acid;
said compound of formula (IV) being in particular prepared according to a process comprising:
i) A step of conversion of a compound of formula (VI) as defined in claim 9 into a compound of formula (V), followed by a step of conversion of the compound of formula (V) into a compound of formula (IV); or ii) A step of conversion of a compound of formula (VI) into a compound of formula (IV);
said compound of formula (VI) being more particular}' obtained from a process comprising:
i) a step of replacement of the hydroxy 1 group of a compound of following formula (VIII):
Figure imgf000156_0001
by a leaving group --Y, Y being in particular an halogen, to give a compound of following formula (VII):
Figure imgf000156_0002
and
ii) A step of contacting the compound of following formula (VII):
with a compound of following formula (IX):
Figure imgf000156_0003
wherein
Ri , R¾ R.3, R4, Z and ¾ are as defined in claim 9,
thereby obtaining a compound of formula (VI).
PCT/EP2018/061050 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins WO2018197727A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP18722450.6A EP3615516B1 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
ES18722450T ES2904366T3 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for their preparation, and their uses as luciferins
JP2019558501A JP7366752B2 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, methods of their preparation and their use as luciferin
CA3061245A CA3061245A1 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
US16/607,058 US11938199B2 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
DK18722450.6T DK3615516T3 (en) 2017-04-28 2018-04-30 IMIDAZOPYRAZINE DERIVATIVES, MANUFACTURING METHOD AND THEIR USES AS LUCIFERINES
EP21200585.4A EP3964501A1 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
JP2023176076A JP2024009933A (en) 2017-04-28 2023-10-11 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17168838.5A EP3395803A1 (en) 2017-04-28 2017-04-28 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
EP17168838.5 2017-04-28

Publications (1)

Publication Number Publication Date
WO2018197727A1 true WO2018197727A1 (en) 2018-11-01

Family

ID=58640785

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2018/061050 WO2018197727A1 (en) 2017-04-28 2018-04-30 Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins

Country Status (7)

Country Link
US (1) US11938199B2 (en)
EP (3) EP3395803A1 (en)
JP (2) JP7366752B2 (en)
CA (1) CA3061245A1 (en)
DK (1) DK3615516T3 (en)
ES (1) ES2904366T3 (en)
WO (1) WO2018197727A1 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111116594A (en) * 2019-12-03 2020-05-08 山东大学 C-6 modified NanoLuc analogue and preparation method and application thereof
CN112513188A (en) * 2018-08-23 2021-03-16 普洛麦格公司 Coelenterazine analogs
CN113214232A (en) * 2021-05-26 2021-08-06 无锡捷化医药科技有限公司 Novel coelenterazine PBI3939 important intermediate and preparation method thereof
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
WO2023025816A1 (en) 2021-08-24 2023-03-02 Institut Pasteur Luminescent based antigen assay
WO2024081914A1 (en) * 2022-10-14 2024-04-18 Promega Corporation Coelenterazine analogues

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2520088A (en) 1948-05-28 1950-08-22 Lilly Co Eli Process of preparing pyrazines
US4046763A (en) 1968-11-08 1977-09-06 Mcneil Laboratories, Incorporated 2,3-Diphenyl-5-ethylpyrazine
WO2010142801A1 (en) 2009-06-11 2010-12-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Indole amide derivatives and related compounds for use in the treatment of neurodegenerative diseases
US20120107849A1 (en) * 2010-11-02 2012-05-03 Klaubert Dieter H Coelenterazine derivatives and methods of using same
WO2012119046A2 (en) 2011-03-02 2012-09-07 Bioenergenix Heterocyclic compounds for the inhibition of pask
US20140302539A1 (en) 2013-04-08 2014-10-09 Jnc Corporation Coelenterazine analogs
US20150212078A1 (en) * 2014-01-29 2015-07-30 Promega Corporation Pro-substrates for live cell applications

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090075309A1 (en) * 2007-09-06 2009-03-19 Gambhir Sanjiv S Bisdeoxycoelenterazine derivatives, methods of use, and BRET2 systems
GB2479847B (en) * 2009-02-09 2014-02-19 Jnc Corp Coelenterazine analogs and manufacturing method thereof
JP6511720B2 (en) * 2013-02-28 2019-05-15 Jnc株式会社 Codon optimized mutant shrimp luciferase gene and method of using the same
US9790537B2 (en) * 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
EP3416968B1 (en) * 2016-02-15 2022-07-27 Promega Corporation Coelenterazine analogues
CN105968114B (en) * 2016-05-27 2018-11-09 山东大学 A kind of coelenterazine analog and the preparation method and application thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2520088A (en) 1948-05-28 1950-08-22 Lilly Co Eli Process of preparing pyrazines
US4046763A (en) 1968-11-08 1977-09-06 Mcneil Laboratories, Incorporated 2,3-Diphenyl-5-ethylpyrazine
WO2010142801A1 (en) 2009-06-11 2010-12-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Indole amide derivatives and related compounds for use in the treatment of neurodegenerative diseases
US20120107849A1 (en) * 2010-11-02 2012-05-03 Klaubert Dieter H Coelenterazine derivatives and methods of using same
WO2012119046A2 (en) 2011-03-02 2012-09-07 Bioenergenix Heterocyclic compounds for the inhibition of pask
US20140302539A1 (en) 2013-04-08 2014-10-09 Jnc Corporation Coelenterazine analogs
US20150212078A1 (en) * 2014-01-29 2015-07-30 Promega Corporation Pro-substrates for live cell applications

Non-Patent Citations (34)

* Cited by examiner, † Cited by third party
Title
ACC. CHEM. RES., 1998, pages 852
ANGEW. CHEM., INT. ED., 2008, pages 3096
ANGEW. CHEM., INT. ED., 2008, pages 6338
BEILSTEIN J. ORG. CHEM., 2012, pages 2004
BER. DTSCH. CHEM. GES., 1902, pages 3772
BULL. ACAD. SCI. USSR DIV. CHEM. SCI. (ENGL. TRANSL.), 1980, pages 458
BULL. ACAD. SCI. USSR DIV. CHEM. SCI. (ENGL. TRANSL.), 1981, pages 466
CHEM. EUR. J., vol. 21, 2015, pages 17158
CHEM. REV., 2006, pages 2651
CHEMISTRY, 2013, pages 14970
COLL. CZECH. CHEM. COMMUN., 1987, pages 2534
ELIEL, E. L.; WILEN, S.H.: "Stereochemistry of Organic Compounds", 1994, WILEY
HETEROCYCLES, 1998, pages 1255
J. AM. CHEM. SOC., 1949, pages 78
J. AM. CHEM. SOC., 1952, pages 1580
J. AM. CHEM. SOC., vol. 78, 1949
J. F. W.: "McOmie in Protective Groups in Organic Chemistry", 1973, PLENUM PRESS
J. LEVI ET. AL.: "Bisdeoxycoelenterazine Derivatives for Improvement of Bioluminescence Resonance Energy Transfer Assays.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 129, 9 December 2007 (2007-12-09), pages 11900 - 11901, XP002771166, DOI: 10.1021/ja073936h *
J. ORG. CHEM., 1961, pages 1907
J. ORG. CHEM., 2006, pages 360
J. ORG. CHEM., 2013, pages 7930
J. ORGANOMET. CHEM., 1999, pages 125
JACQUES, J ET AL.: "Enantiomers, Racemates, and Resolutions", 1981, WILEY
LAB. CHIPS, vol. 13, 2013, pages 2210 - 2251
ORG. BIOMOL. CHEM., 2012, pages 524
ORG. LETT., 2016, pages 4128
ORG. PROCESS. RES. DEV., 2007, pages 1069
ORG. PROCESS. RES. DEV., vol. 21, 2017, pages 346
R.C. LAROCK: "Comprehensive Organic Transformations", 1999, WILEY-VCH PUBLISHERS
SYNLETT, 2003, pages 1801
T.W. GREENE; P. G. M. WUTS: "Protective Groups in Organic Chemistry", 1999, JOHN WILEY AND SONS
TETRAHEDRON LETT., 1977, pages 2685
TETRAHEDRON, 1998, pages 9701
TETRAHEDRON, 2011, pages 9405

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112513188A (en) * 2018-08-23 2021-03-16 普洛麦格公司 Coelenterazine analogs
CN112513188B (en) * 2018-08-23 2024-01-26 普洛麦格公司 Coelenterazine analogs
CN111116594A (en) * 2019-12-03 2020-05-08 山东大学 C-6 modified NanoLuc analogue and preparation method and application thereof
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
WO2021219544A1 (en) 2020-04-27 2021-11-04 Institut Pasteur Luciferase linked immunosorbent assay
CN113214232A (en) * 2021-05-26 2021-08-06 无锡捷化医药科技有限公司 Novel coelenterazine PBI3939 important intermediate and preparation method thereof
CN113214232B (en) * 2021-05-26 2022-07-12 无锡捷化医药科技有限公司 Coelenterazine PBI3939 intermediate and preparation method thereof
WO2023025816A1 (en) 2021-08-24 2023-03-02 Institut Pasteur Luminescent based antigen assay
WO2024081914A1 (en) * 2022-10-14 2024-04-18 Promega Corporation Coelenterazine analogues

Also Published As

Publication number Publication date
JP2020521724A (en) 2020-07-27
EP3395803A1 (en) 2018-10-31
DK3615516T3 (en) 2022-01-17
JP7366752B2 (en) 2023-10-23
ES2904366T3 (en) 2022-04-04
EP3615516A1 (en) 2020-03-04
US11938199B2 (en) 2024-03-26
JP2024009933A (en) 2024-01-23
EP3964501A1 (en) 2022-03-09
CA3061245A1 (en) 2018-11-01
EP3615516B1 (en) 2021-11-17
US20200129644A1 (en) 2020-04-30

Similar Documents

Publication Publication Date Title
EP3615516B1 (en) Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
DE60221392T2 (en) NEW PYROL DERIVATIVES AS PHARMACEUTICAL AGENTS
CN102762549A (en) Phthalazinone derivative, and preparation method and pharmaceutical use thereof
EA036630B1 (en) Bicyclic derivatives
SU604496A3 (en) Method of obtaining heterogeneous ring compounds
Dofe et al. Synthesis, antimicrobial evaluation, and molecular docking studies of novel chromone based 1, 2, 3-triazoles
WO2022135572A1 (en) Five-membered ring derivative and medical use thereof
SA516370448B1 (en) New indolizine compounds, a process for their preparation and pharmaceutical compositions containing them
Kutyashev et al. Different behavior of azomethine ylides derived from 11 H-indeno [1, 2-b] quinoxalin-11-one and proline/sarcosine in reactions with 3-nitro-2 H-chromenes
Kutyashev et al. 3-Nitro-2-phenyl-2-trifluoromethyl-2 H-chromenes in reactions with azomethine ylides from isatins and (thia) proline: synthesis of spiro [chromeno (thia) pyrrolizidine-11, 3'-oxindoles]
Savchuk et al. Efficient one-step synthesis of 3-aryl-2-pyridones from 6-aryl-1, 2, 4-triazin-5-ones
WO2010133794A1 (en) Anticancer compound and pharmaceutical composition containing the same
Hajós et al. Synthesis and structural study of azidonaphtho-as-triazines." Annelation effect" in azide-tetrazole equilibria
Kurbatov et al. The nitroolefinic behavior of 4-nitrobenzodifuroxan
Uvarova et al. Assembly of isoxazol-5-one with 2-unsubstituted imidazole N-oxides and aldehydes
Glushkov et al. The synthesis of 3-(het) aryl-6, 7-dihydro-5 H-[1, 2, 4]-triazolo [3, 4-a][2] benzazepines
Syrota et al. An efficient method for accessing carboannulated and functionalized [1, 2, 3] triazolo [4, 5-b] pyridines
Hikem‐Oukacha et al. New synthesis and reactivity of 3‐bromoacetyl‐4‐hydroxy‐6‐methyl‐2H‐pyran‐2‐one with binucleophilic amines
Khazhieva et al. Synthesis and fungicidal activity of monocyclic and fused 1, 2, 3-triazolium-5-olates
Khlebnikova et al. Synthesis of fluorine-containing 6, 7-dihydrobenzisoxazolones from 2-(fluorobenzoyl) cyclohexane-1, 3-diones and their methyl enol ethers
El-Essawy et al. Synthesis of new isolated and fused tri-and tetracyclic pyridine derivatives
Kuznetsova et al. A simple synthesis of 2-{2-[(arylmethylidene) amino]-indazol-3-yl} malonate esters
Cheng et al. The Synthesis and Herbicidal Activity of 5‐(Substituted‐phenyl)‐4, 6‐dioxo− 4, 5, 6, 7‐tetrahydropyrazolo [3, 4‐d] pyrimidines
Muzychka et al. Pyrrolo [2, 3-d] pyrimidine derivatives in the synthesis of a novel heterocyclic system 2a, 5a, 7-triazaacenaphthylene
Bogdanov et al. Effect of Structure of 1-Substituted Isatins on Direction of Their Reactions with Some Acetohydrazide Ammonium Derivatives

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18722450

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 3061245

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2019558501

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2018722450

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2018722450

Country of ref document: EP

Effective date: 20191128