WO2018125021A1 - Method of manufacture of fish food pellets for multi-age fish groups - Google Patents
Method of manufacture of fish food pellets for multi-age fish groups Download PDFInfo
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- WO2018125021A1 WO2018125021A1 PCT/UA2017/000078 UA2017000078W WO2018125021A1 WO 2018125021 A1 WO2018125021 A1 WO 2018125021A1 UA 2017000078 W UA2017000078 W UA 2017000078W WO 2018125021 A1 WO2018125021 A1 WO 2018125021A1
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- Prior art keywords
- fish
- hydrolyzate
- feed
- hydrolysis
- production
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- 238000002360 preparation method Methods 0.000 claims abstract description 10
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- 235000018102 proteins Nutrition 0.000 description 8
- 241000881711 Acipenser sturio Species 0.000 description 6
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- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
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- 210000000514 Hepatopancreas Anatomy 0.000 description 1
- 229960000310 ISOLEUCINE Drugs 0.000 description 1
- 210000000936 Intestines Anatomy 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine zwitterion Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
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- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000530454 Litopenaeus schmitti Species 0.000 description 1
- 102000035348 Neutral proteases Human genes 0.000 description 1
- 108091005544 Neutral proteases Proteins 0.000 description 1
- 241000238124 Paralithodes camtschaticus Species 0.000 description 1
- 229960005190 Phenylalanine Drugs 0.000 description 1
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000975357 Salangichthys microdon Species 0.000 description 1
- 240000008529 Triticum aestivum Species 0.000 description 1
- 235000019742 Vitamins premix Nutrition 0.000 description 1
- 235000005042 Zier Kohl Nutrition 0.000 description 1
- 238000005047 biotechnology Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
Abstract
The invention relates to the agricultural sector, in particular to the feed industry, specifically the production of feed for fish. The object of the invention is the method of production of mixed fish food pellet for multi-age groups of fish, which includes the preparation of hydrolyzate of animal by product at a temperature of 50-60°C, combination of a hydrolyzate without separating the unprocessed protein in the feed mixture, mixing the components, moisturizing the ingredients due to the moisture of the hydrolyzate, extruding, drying, cooling, sieving, packaging, labeling and storage, differs by the fact that the process of hydrolysis is carried out without water, the mass fraction of the enzyme is 0.01-1% to the minced weight, the duration of the hydrolysis is 5-6 hours, the degree of hydrolysis is 10-45%, while the hydrolyzate is added into nutrition mixture in an amount of 25-50% and the nutrition is dried at the temperature of 90°C.
Description
METHOD OF MANUFACTURE OF FISH FOOD PELLETS
FOR MULTI-AGE FISH GROUPS
The invention relates to the agricultural sector, in particular to the feed industry, specificaly the production of fish food.
A well-known process (Patent No. 2297154 of the Patent Office of 20.04.2002. A method for preparing feed for young sturgeon), which includes mixing components of animal and vegetable origin, fat and multivitamin premix, characterized in that the feed mixture is fed anhydrolyzate of animal origin with the addition of Organic and inorganic acids (pH 4,0-5,0) in the ratio of 100 2, with the addition of homogenate of enzymes, the holding time of hydrolyzate is 7 days, the final depth of hydrolysis is 55-75%.
The disadvantage of the known process is suitability only for sturgeon fish, the presence of fish meal in the feed, the addition of inorganic acids during hydrolysis, the 7 day lenght of process, the separation of the unprocessed protein, which reduces the yield of the hydrolyzate, and the operation of drying the hydrolyzate.
Also known process (Niu J. et al., Effects of graded replacement of fish meal by fish protein hydrolysate on the growth performance of early post-larval Pacific white shrimp (Litopenaeus vannamei, Boone) // Journal of Applied Animal Research. - 2014. - T. 42. - No. 1. - P. 6-15.), In which it is proposed to improve the feed for white shrimp hydrolyzate from gold nitepero breeding. This supplement improves growth, reduces feed costs, digestion, congenital immunity and disease resistance.
The disadvantage of the known process is the suitability for use only for shrimp, the presence of fish meal in the feed, the addition of sodium hydroxide to
adjust the pH of the hydrolyzate, energy-consuming operations of purification and drying of the hydrolyzate.
Patent of the Russian Federation No.: 2490932, publication 10.05.2013, Method of preparation of feed for young sturgeon fish, includes the mixing of components of animal and vegetable origin, fat and multivitamin premix, characterized by feeding fish protein into the mixture, hydrolyzed by proteolytic enzymes Containing polypeptides with a molecular weight of 1000-1300 Da, in an amount of 7-10% by weight of the feed, and after mixing the components of the feed, extrusion is carried out at a temperature of 100-120 C° and a pressure of 7-15 MPa.
The disadvantage of this method is the suitability only for sturgeon, the presence of fish meal in the feed, the operation of drying the hydrolyzate.
A well-known process (Russian Patent No. 2366265, published September 10, 2009, Preparation of Forage for Early Young Salmon Fish), consisting in obtaining a proteolytic enzymatic protein hydrolyzate without separating the unprocessed protein, mixing with vegetable components, fish oil, vitamin-mineral premix, and Drying the mixture until the moisture content of the finished product is not more than 10%, characterized in that the raw materials for the preparation of the hydrolyzate are used to treat the hydrobionts, proteolytic enzymes hepatopancreas Kamchatka crab, preparation of protein hydrolyzate is carried out at a ratio of masses of raw materials and water 1 : 1 , masses of raw materials and proteolytic agent: gepatopankreas-raw or a preparation obtained from it - 1 : 0,010-1 : 0,030 and 1 : 0,001 -1 : 0.003, respectively, at a temperature of 50-55° C and a natural pH within 1.5-2 h until the degree of hydrolysis is 12-20%, prepared for the introduction of protein hydrolyzate with a mass fraction of water up to 90% are mixed until a homogeneous mass is obtained with minced fish , Cooked from fillets of low value fish, with waste and flour-grinding production: wheat germ and /or cabbage and algae flour - vegetable components, premix. A disadvantage of the known method is suitability for salmon only, the introduction of water into the hydrolyzate, drying of the hydrolyzate, the lack of buoyancy in the formed pellets.
A Similar process is the method (Patent of the Russian Federation No. 2159556, dated 27.1 1.2000, For preparation of feed for young sturgeon fish), which contains a mixture of fish meal, yeast, dried milk, soy bean, fish oil and vitamin premix, but fish meal is replaced by hydrolyzate without the separation of unprocessed protein with a depth of hydrolysis of non-protein nitrogen 23- 41 % of the total nitrogen of the feedstock, with hydrolysis carried out at a dose of 30% of the weight of minced meat and at a temperature of 55 - 60 C°, PH 6.6 on the basis of prototype or with the addition of proteolytic enzymes, the hydrolyzate obtained without separation unprocessed protein with humidity of 35 - 40% is administered in an amount of 50- 60% by weight of animal by product. Humidification of feed ingredients occurs due to the moisture contained in the hydrolyzate.
The disadvantages of these methods are the introduction of 30% of water during the hydrolysis, separate inactivation of the hydrolyzate, drying of the liquid hydrolyzate, because the process requires significant electricity consumption, low drying temperature of the feed, which increases the duration of the process.
The basis of this invention is the task of creating a new process for the production of mixed fish food pellets, taking into account the shortcomings of existing process.
The process for production of mixed fish food pellets for multi-age groups of fish are characterized by the following research.
Research 1. For the production of feed pellets for sturgeon, take small-scale freshwater raw material - caraway (Carassius gibelio) cooled, weighing up to 200g , as well as waste from the dismantling of rainbow trout. Grind on a plate with a diameter of holes of a grill of 3mm. Make 0.2% of the weight of the enzyme preparation neutral protease (Beijing Challenge Bio-technology Limited Company, the production of the People's Republic of China), without the additional substances for pH regulation. Water is not introduced into the system. Hydrolyze with constant stirring for 5.5 h at 55 °C. The resulting hydrolyzate with a degree of hydrolysis of 35.13%, without separation of the unfertilized protein residue, is added to the fodder mixture in an amount of 36%. The resulting moisture of the
feed mass is extruded, directed to convection drying at a temperature of 90 ° C, cooled, sifted, packaged, marked, stored.
Research 2. For the production of compound feed for salmon, including rainbow trout (Oncorhynchus mykiss), take low-value raw material - (Clupeonella delicatula) frozen whitebait. It is thawed, crushed on a plate with a diameter of the holes of the grating 3 mm. Add 0,5% to the weight of the domestic enzyme preparation "Protorysin" (LLC "TD Enzim"). Water is not introduced into the system. Hydrolyze with constant stirring for 5 hours at a temperature of 53 ° C. The resulting hydrolyzate with a hydrolysis of 28.87%) without separation of the unprocessed protein is added to the feed mixture in the amount of 40%. The resulting moisture of the feed mass is extruded, directed to convection drying at a temperature of 90 0 C, cooled, sifted, packaged, marked, stored.
A series of laboratory studies were conducted for the received feed. As a control, imported fodder of Danish production was used.
The results of the determination of the amino acid composition of feed (the O.V. Palladin Institute of Biochemistry) confirm that the content of amino acids indispensable to fish is the highest in feeds developed according to the process, and the smallest values are fixed for control.
Table 1
Results of amino acid analysis of feed samples
M of amino acids er 100 m sam le
8. Proline 2,900 2,933
9. Glycine 3,514 2,995
10. Alanin 3,891 3,015
1 1. Cystine 1,048 0,765
12. Valine 2,270 2,398
13. Methionine 0,71 0 1,037
14. Isoleucine 1 ,461 1,3 13
15. Leucine 4,447 4,147
16. Tyrosine 1 ,308 1,261
17. Phenylalanine 2,596 2,445
Sum 48,309 46,295
For the proposed fish food pellets, studies of relative biological value have been carried out using Infuzoria- Tetrachimena pyriformis.
Relative biological value is an integral indicator of the physiological effect of feed on the organism of fish. The results of the research (Table 2) show that the domestic production of feed has a higher value of this indicator by 6.5% than the existing process.
Table 2
Relative biological value of fish food pellet protein For trout
Production tests were carried out within 10 months. Research Production Center "Trout" LLC.
Objects of study of fish-rod indicators were specimens of rainbow trout fish (Oncorhynchus mykiss) of different live weight.
Investigation of the size and mass composition of trout (50 fish species) made it possible to establish that for the fish grown on the proposed feed, the intestines make up 14.1 %, in the control feed - 15.7% of the total mass of fish. This convincingly suggests a more proportional development of the body of fish when feeding pellets produced by the proposed method and increasing the efficiency of production in the case of the implementation of cooled, salted, smoked products from grown fish.
The analysis of the results of the study of fish indicators and the physiological state grown on various mixed fish pellets (Table 3) shows that the proposed method of production allows for the receipt of feed for fish, which is not inferior to expensive imported product in terms of survival, weight gain, pellet costs.
Table 3
Results of the study of fish and indicators
limits sometimes form clumps
9. liver Signs of tissue Signs of tissue degeneration were not degeneration detected have not been detected, but in some cases there is a slight venous congestion in the liver
10. Epizootically significant parasites Not found Not found
1 1. growth, % initial weight 10,4±0,2 10,5±0,2
12. Food cost 1 1
13. Survival, % 98,4±0,1 92.1±0,4
The technical result of the proposed method for the production of mixed fish feed pellets for multi-aged fish groups is to increase the efficiency of production of feed pellets, reduce the cost of electricity, increase their biological value, and improve the condition of fish grown on these feed pellets. The implementation of the proposed solution allows you to obtain competitive feed that has a long decay in water, low cost and long shelf life.
A comparative characteristic of the features of the analogues and the present invention
Claims
Method of production of mixed fish food pellet for multi-age groups of fish, which includes the preparation of hydrolyzate of animal by product at a temperature of 50-60 0 C, combination of a hydrolyzate without separating the unprocssed protein in the feed mixture, mixing the components, moisturizing the ingredients due to the moisture of the hydrolyzate, extruding, drying, cooling, sieving, packaging, labeling and storage, differs by the fact, that the process of hydrolysis is carried out without water, the mass fraction of the enzyme is 0.01 -1% to the minced weight, the duration of the hydrolysis is 5-6 hours, the degree of hydrolysis is 10-45%, while the hydrolyzate is added into Nutrition mixture in an amount of 25-50%) and the nutrition is dried at the temperature of 90 0 C.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UA201613649 | 2016-12-30 | ||
| UAA201613649 | 2016-12-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2018125021A1 true WO2018125021A1 (en) | 2018-07-05 |
Family
ID=62710054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/UA2017/000078 WO2018125021A1 (en) | 2016-12-30 | 2017-08-02 | Method of manufacture of fish food pellets for multi-age fish groups |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2018125021A1 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2173532C1 (en) * | 2000-04-03 | 2001-09-20 | Боева Нэля Петровна | Feed fish flour production method |
| CN105154505A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Preparation method of feed grade ocean fish oligopeptide meal |
-
2017
- 2017-08-02 WO PCT/UA2017/000078 patent/WO2018125021A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2173532C1 (en) * | 2000-04-03 | 2001-09-20 | Боева Нэля Петровна | Feed fish flour production method |
| CN105154505A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Preparation method of feed grade ocean fish oligopeptide meal |
Non-Patent Citations (1)
| Title |
|---|
| VEGA R.E. ET AL.: "Enzymic hydrolysis of fish offal without added water", JOURNAL OF FOOD ENGINEERING, vol. 8, no. 3, 28 October 1988 (1988-10-28), pages 201 - 215, XP055517034 * |
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