WO2018064968A1 - Method for producing edible cell - Google Patents
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- WO2018064968A1 WO2018064968A1 PCT/CN2017/104977 CN2017104977W WO2018064968A1 WO 2018064968 A1 WO2018064968 A1 WO 2018064968A1 CN 2017104977 W CN2017104977 W CN 2017104977W WO 2018064968 A1 WO2018064968 A1 WO 2018064968A1
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- edible
- cells
- cell culture
- animal
- edible cells
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 238000004113 cell culture Methods 0.000 claims abstract description 27
- 210000001519 tissues Anatomy 0.000 claims abstract description 21
- 238000000338 in vitro Methods 0.000 claims abstract description 14
- 210000004027 cells Anatomy 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 7
- 235000013361 beverage Nutrition 0.000 claims description 6
- 235000013373 food additive Nutrition 0.000 claims description 6
- 239000002778 food additive Substances 0.000 claims description 6
- 235000021067 refined food Nutrition 0.000 claims description 6
- 210000002826 Placenta Anatomy 0.000 claims description 4
- 210000003205 Muscles Anatomy 0.000 claims description 3
- 125000000837 carbohydrate group Chemical group 0.000 claims description 3
- 210000004748 cultured cells Anatomy 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 235000003599 food sweetener Nutrition 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000001954 sterilising Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000003765 sweetening agent Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 210000001835 Viscera Anatomy 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 230000029087 digestion Effects 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract 1
- 206010060945 Bacterial infection Diseases 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 2
- 235000013622 meat product Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 229940088597 Hormone Drugs 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 210000004185 Liver Anatomy 0.000 description 1
- 210000004072 Lung Anatomy 0.000 description 1
- 241000283898 Ovis Species 0.000 description 1
- 210000002784 Stomach Anatomy 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A method for producing edible cells, comprising performing in vitro cell culture of isolated tissues of animals to obtain the edible cells that can be directly eaten and consumed by a human body. Not only the production cycle is accelerated, but also the safety of the produced edible cells can be ensured and the edible cells are not easily infected by bacteria since the culture environment can be strictly controlled for in vitro cell culture. Furthermore, since the human body can directly eat the edible cells, the digestion and absorption efficiency is greatly increased as compared to a case in which animal tissues are eaten.
Description
本发明属于食品领域，涉及一种可食用细胞的生产方法。The invention belongs to the field of food and relates to a method for producing edible cells.
目前人类可食用的肉类产品通常直接取材于动物的组织，例如直接通过宰杀切割牛肉、猪肉、家禽、羊肉或鱼等根据需要获取可食用部位。这种方法非常低效，且存在诸多弊端，一方面，动物饲养需要消耗大量的粮食，且饲养周期较长；另一方面，为加快动物的饲养，目前人们广泛采用生长激素加速动物的生长，而长期食用激素培养的肉对于人体的健康会造成潜在的威胁；同时，肉类食品存在食品污染的问题，当肉类因动物摄入有害物质、或储藏过程中感染有害物质，均会对人类的健康造成威胁。除上述不利因素外，人类直接食用动物的组织，还会存在消化吸收效率不高的问题。At present, human edible meat products are usually taken directly from animal tissues, for example, by slaughtering cut beef, pork, poultry, lamb or fish, etc., as needed, to obtain edible parts. This method is very inefficient, and has many drawbacks. On the one hand, animal feeding needs to consume a large amount of food, and the feeding cycle is long; on the other hand, in order to speed up the breeding of animals, growth hormone is widely used to accelerate the growth of animals. The meat cultured by long-term consumption of hormones poses a potential threat to human health. At the same time, there is a problem of food contamination in meat products. When meat is ingested by harmful substances in animals or infected with harmful substances during storage, it will be human. The health poses a threat. In addition to the above unfavorable factors, human direct consumption of animal tissue, there will be problems of low digestion and absorption efficiency.
发明内容Summary of the invention
本发明的目的在于提供一种可食用细胞的生产方法，旨在解决现有技术中直接食用动物组织导致的对人类健康造成潜在威胁，以及消化吸收效率低下的问题。The object of the present invention is to provide a method for producing edible cells, which aims to solve the problem of potential threat to human health caused by direct consumption of animal tissues in the prior art, and the inefficiency of digestion and absorption.
为解决上述技术问题，本发明提供了一种可食用细胞的生产方法，将动物的离体组织利用体外细胞培养技术进行培养，得到可食用细胞。In order to solve the above technical problems, the present invention provides a method for producing edible cells, which is cultured by using an in vitro cell culture technique to obtain edible cells.
优选的，所述动物的离体组织包括动物的离体胎盘、肌肉和内脏。Preferably, the isolated tissue of the animal comprises the isolated placenta, muscle and internal organs of the animal.
优选的，所述生产方法还包括对所述可食用细胞进行深加工制成可食用的深加工食品。Preferably, the method of production further comprises further processing the edible cells to form an edible deep processed food.
进一步的，所述可食用的深加工食品为饮品，所述生产方法具体包括：
Further, the edible deep processed food is a drink, and the production method specifically comprises:
将动物的离体组织利用体外细胞培养技术进行培养，得到细胞培养产物；The isolated tissue of the animal is cultured by an in vitro cell culture technique to obtain a cell culture product;
取所述细胞培养产物与食品添加剂和水进行配比混合，经杀菌后得到所述饮品。The cell culture product is mixed with a food additive and water, and the beverage is obtained after sterilization.
进一步的，所述细胞培养产物包括培养后的细胞及其营养液。Further, the cell culture product includes the cultured cells and their nutrient solution.
进一步的，所述食品添加剂包括甜味剂和乳化剂，所述细胞培养产物占所述饮品的质量百分比为0.01％-98％。Further, the food additive comprises a sweetener and an emulsifier, and the cell culture product accounts for 0.01% to 98% by mass of the beverage.
进一步的，所述生产方法还包括对所述可食用细胞进行提取，得到用于食品添加的提取物。Further, the production method further comprises extracting the edible cells to obtain an extract for food addition.
优选的，所述提取物为糖类、蛋白质、核酸或脂肪。Preferably, the extract is a saccharide, a protein, a nucleic acid or a fat.
与现有技术相比，本发明提供的一种可食用细胞的生产方法，通过对离体动物的组织进行体外细胞培养，得到可供人体直接食用摄取的可食用细胞，一方面加快了生产的周期，另一方面，由于体外细胞培养可严格控制培养环境，可以保证生产得到的可食用细胞的安全性，不易受到细菌感染。同时，人体可直接食用所述可食用细胞，与食用动物组织相比，其消化吸收效率大大提高。Compared with the prior art, the present invention provides a method for producing edible cells, which can obtain edible cells for direct consumption by human body by in vitro cell culture of tissues of isolated animals, and on the other hand, accelerate production. Cycle, on the other hand, because in vitro cell culture can strictly control the culture environment, it can ensure the safety of edible cells produced and is not susceptible to bacterial infection. At the same time, the human body can directly consume the edible cells, and the digestion and absorption efficiency is greatly improved compared with the edible animal tissues.
以下结合具体实施例对本发明提出的一种可食用细胞的生产方法作进一步详细说明。根据下面说明和权利要求书，本发明的优点和特征将更清楚。Hereinafter, a method for producing edible cells proposed by the present invention will be further described in detail in conjunction with specific examples. Advantages and features of the present invention will be apparent from the description and appended claims.
本发明提供了一种可食用细胞的生产方法，将动物的离体组织利用体外细胞培养技术进行培养，得到可食用细胞。将动物新鲜的活的离体组织利用体外细胞培养技术进行培养，培养后得到分散的多个细胞，这些细胞可直接供人体摄入食用，通过这种方法，一方面加快了生产的周期，另一方面，由
于体外细胞培养可严格控制培养环境，可以保证生产得到的可食用细胞的安全性，不易受到细菌感染。同时，人体可直接食用所述可食用细胞，与食用动物组织相比，其消化吸收效率大大提高。The invention provides a method for producing edible cells, wherein the isolated tissue of the animal is cultured by using an in vitro cell culture technique to obtain edible cells. The fresh live ex vivo tissue of the animal is cultured by in vitro cell culture technology, and after the culture, a plurality of dispersed cells are obtained, and the cells can be directly consumed by the human body. By this method, on the one hand, the production cycle is accelerated, and on the other hand, the production cycle is accelerated. On the one hand, by
In vitro cell culture can strictly control the culture environment, can ensure the safety of edible cells produced, and is not susceptible to bacterial infection. At the same time, the human body can directly consume the edible cells, and the digestion and absorption efficiency is greatly improved compared with the edible animal tissues.
优选的，所述动物的离体组织包括动物的离体胎盘(例如牛、羊、猪等动物的胎盘)、肌肉和内脏(动物的离体肝脏、心脏、胃、肺等)等。本领域技术人员应当注意的是，这里对于离体组织的列举并不完全，理论上，任何动物的组织均可以通过上述方案进行培养，得到可供人体直接食用的细胞。Preferably, the isolated tissue of the animal includes an isolated placenta of the animal (such as a placenta of an animal such as a cow, a sheep, a pig, etc.), a muscle and a viscera (an isolated liver of the animal, a heart, a stomach, a lung, etc.) and the like. It should be noted by those skilled in the art that the enumeration of ex vivo tissue is not complete here. Theoretically, any animal tissue can be cultured by the above scheme to obtain cells which can be directly consumed by the human body.
优选的，所述生产方法还包括对所述可食用细胞进行深加工制成可食用的深加工食品。在本实施例中，所述可食用细胞除可直接食用外，还可以通过深加工的方法，进一步对所述可食用细胞进行深加工，一方面可以增加产品的种类，提高产品的附加值，另一方面，通过深加工，使得可食用细胞的营养价值能够充分发挥出来。Preferably, the method of production further comprises further processing the edible cells to form an edible deep processed food. In this embodiment, in addition to being directly edible, the edible cells may further deep-process the edible cells by deep processing, on the one hand, increasing the type of the product, and increasing the added value of the product, and the other In terms of deep processing, the nutritional value of edible cells can be fully utilized.
进一步的，所述可食用的深加工食品为饮品，所述生产方法具体包括：Further, the edible deep processed food is a drink, and the production method specifically comprises:
将动物的离体组织利用体外细胞培养技术进行培养，得到细胞培养产物；The isolated tissue of the animal is cultured by an in vitro cell culture technique to obtain a cell culture product;
取所述细胞培养产物与食品添加剂和水进行配比混合，经杀菌后得到所述饮品。The cell culture product is mixed with a food additive and water, and the beverage is obtained after sterilization.
进一步的，所述细胞培养产物包括培养后的细胞及其营养液。Further, the cell culture product includes the cultured cells and their nutrient solution.
进一步的，所述食品添加剂包括甜味剂和乳化剂，所述细胞培养产物占所述饮品的质量百分比为0.01％-98％。细胞培养产物的百分比可根据需要自行调整。Further, the food additive comprises a sweetener and an emulsifier, and the cell culture product accounts for 0.01% to 98% by mass of the beverage. The percentage of cell culture product can be adjusted as needed.
进一步的，所述生产方法还包括对所述可食用细胞进行提取，得到用于食品添加的提取物。在本实施例中，所述可食用细胞还可进行提取，将提取物用于食品添加领域中。Further, the production method further comprises extracting the edible cells to obtain an extract for food addition. In this embodiment, the edible cells can also be extracted, and the extract is used in the field of food addition.
优选的，所述提取物为糖类、蛋白质、核酸或脂肪。
Preferably, the extract is a saccharide, a protein, a nucleic acid or a fat.
本发明提供的一种可食用细胞的生产方法，通过对离体动物的组织进行体外细胞培养，得到可供人体直接食用摄取的可食用细胞，一方面加快了生产的周期，另一方面，由于体外细胞培养可严格控制培养环境，可以保证生产得到的可食用细胞的安全性，不易受到细菌感染。同时，人体可直接食用所述可食用细胞，与食用动物组织相比，其消化吸收效率大大提高。The invention provides a method for producing edible cells, which can obtain edible cells which can be directly ingested by the human body by in vitro cell culture of the tissues of the isolated animals, on the one hand, the production cycle is accelerated, on the other hand, In vitro cell culture can strictly control the culture environment, which can ensure the safety of edible cells produced and is not susceptible to bacterial infection. At the same time, the human body can directly consume the edible cells, and the digestion and absorption efficiency is greatly improved compared with the edible animal tissues.
上述描述仅是对本发明较佳实施例的描述，并非对本发明范围的任何限定，本发明领域的普通技术人员根据上述揭示内容做的任何变更、修饰，均属于权利要求书的保护范围。
The above description is only for the description of the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention. Any changes and modifications made by those skilled in the art in light of the above disclosure are all within the scope of the appended claims.
Claims (8)
- 一种可食用细胞的生产方法，其特征在于，将动物的离体组织利用体外细胞培养技术进行培养，得到可食用细胞。A method for producing edible cells, characterized in that an isolated tissue of an animal is cultured by an in vitro cell culture technique to obtain edible cells.
- 根据权利要求1所述的可食用细胞的生产方法，其特征在于，所述动物的离体组织包括动物的离体胎盘、肌肉和内脏。The method of producing edible cells according to claim 1, wherein the ex vivo tissue of the animal comprises an isolated placenta, muscle and viscera of the animal.
- 根据权利要求1所述的可食用细胞的生产方法，其特征在于，所述生产方法还包括对所述可食用细胞进行深加工制成可食用的深加工食品。The method of producing edible cells according to claim 1, wherein the production method further comprises deep processing the edible cells to form an edible deep processed food.
- 根据权利要求3所述的可食用细胞的生产方法，其特征在于，所述可食用的深加工食品为饮品，所述生产方法具体包括：The method for producing edible cells according to claim 3, wherein the edible deep processed food is a drink, and the production method comprises:将动物的离体组织利用体外细胞培养技术进行培养，得到细胞培养产物；The isolated tissue of the animal is cultured by an in vitro cell culture technique to obtain a cell culture product;取所述细胞培养产物与食品添加剂和水进行配比混合，经杀菌后得到所述饮品。The cell culture product is mixed with a food additive and water, and the beverage is obtained after sterilization.
- 根据权利要求4所述的可食用细胞的生产方法，其特征在于，所述细胞培养产物包括培养后的细胞及其营养液。The method of producing edible cells according to claim 4, wherein the cell culture product comprises the cultured cells and the nutrient solution thereof.
- 根据权利要求4所述的可食用细胞的生产方法，其特征在于，所述食品添加剂包括甜味剂和乳化剂，所述细胞培养产物占所述饮品的质量百分比为0.01％-98％。The method of producing edible cells according to claim 4, wherein the food additive comprises a sweetener and an emulsifier, and the cell culture product accounts for 0.01% to 98% by mass of the beverage.
- 根据权利要求1所述的可食用细胞的生产方法，其特征在于，所述生产方法还包括对所述可食用细胞进行提取，得到用于食品添加的提取物。The method of producing edible cells according to claim 1, wherein the production method further comprises extracting the edible cells to obtain an extract for food addition.
- 根据权利要求7所述的可食用细胞的生产方法，其特征在于，所述提取物为糖类、蛋白质、核酸或脂肪。 The method of producing edible cells according to claim 7, wherein the extract is a saccharide, a protein, a nucleic acid or a fat.
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| CN201610879200.8A CN106282093A (en) | 2016-10-08 | 2016-10-08 | A kind of production method of edible cell |
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| AU2015214092B2 (en) * | 2014-02-05 | 2018-11-15 | Fork & Goode, Inc. | Dried food products formed from cultured muscle cells |
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| US11277979B2 (en) | 2013-07-31 | 2022-03-22 | Ecovative Design Llc | Mycological biopolymers grown in void space tooling |
| US11420366B2 (en) | 2013-10-14 | 2022-08-23 | Ecovative Design Llc | Method of manufacturing a stiff engineered composite |
| US11505779B2 (en) | 2016-03-01 | 2022-11-22 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
| US11359074B2 (en) | 2017-03-31 | 2022-06-14 | Ecovative Design Llc | Solution based post-processing methods for mycological biopolymer material and mycological product made thereby |
| US11266085B2 (en) | 2017-11-14 | 2022-03-08 | Ecovative Design Llc | Increased homogeneity of mycological biopolymer grown into void space |
| US11293005B2 (en) | 2018-05-07 | 2022-04-05 | Ecovative Design Llc | Process for making mineralized mycelium scaffolding and product made thereby |
| US11343979B2 (en) | 2018-05-24 | 2022-05-31 | Ecovative Design Llc | Process and apparatus for producing mycelium biomaterial |
| US11359174B2 (en) | 2018-10-02 | 2022-06-14 | Ecovative Design Llc | Bioreactor paradigm for the production of secondary extra-particle hyphal matrices |
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