WO2018036033A1 - pL 级超微量补液装置及方法 - Google Patents

pL 级超微量补液装置及方法 Download PDF

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Publication number
WO2018036033A1
WO2018036033A1 PCT/CN2016/110551 CN2016110551W WO2018036033A1 WO 2018036033 A1 WO2018036033 A1 WO 2018036033A1 CN 2016110551 W CN2016110551 W CN 2016110551W WO 2018036033 A1 WO2018036033 A1 WO 2018036033A1
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liquid
micro
connector
tube
capillary
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French (fr)
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张勤
范吉斌
刘嘉超
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South China University of Technology SCUT
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South China University of Technology SCUT
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05C—APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05C11/00—Component parts, details or accessories not specifically provided for in groups B05C1/00 - B05C9/00
    • B05C11/10—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material
    • B05C11/105—Storage, supply or control of liquid or other fluent material; Recovery of excess liquid or other fluent material by capillary action, e.g. using wicks

Definitions

  • the invention relates to the technical field of spotting, in particular to a liquid refilling device and a method for micro-assembly of a pL (picoliter) grade ultra-micro spotting platform.
  • the invention proposes a new type of micro-continuous rehydration device based on the ultra-micro spotting method proposed by Zhang Qin of South China University of Technology, which solves the problem of the original spotting method and the inability to continuously replenish liquid.
  • the ultra-micro spotting method is to use a pipette needle to pass through the liquid in the glass microtube, and the microdroplet adsorbed by the tip of the pipetting needle realizes ultra-micro spotting.
  • the storage of the spotted liquid is located in the glass microtube, and the principle is to fill the glass microtube with liquid by capillary phenomenon.
  • the glass microtube device for realizing the liquid storage glass microtube by capillary phenomenon is limited by the storage principle and the influence of its own structure, the capillary cannot store a large amount of liquid, and the real-time micro-continuous rehydration of the capillary cannot be realized. Therefore, in the original ultra-micro spotting device, the spotting action cannot be continuously performed, and it is necessary to stop the spotting action and replenish the liquid. Due to this limitation, the original method cannot meet the requirements for continuous operation of the ultra-micro spotting platform, and is only applicable to a very small number of discontinuous spotting applications.
  • the method proposed by the invention can realize the micro-continuous rehydration of the ultra-micro spotting device; on the basis of retaining the glass microtubule device which relies on the capillary phenomenon to realize the liquid storage, a real-time rehydration device is invented by relying on the principle of the connector. Solved the problem that the original ultra-micro spotting platform could not work continuously.
  • the present invention designs and invents a pL-level ultra-micro replenishing device and method based on the principle of the connector, while retaining the glass microtube device which relies on the capillary phenomenon to realize liquid storage.
  • the object of the invention is achieved at least by one of the following technical solutions.
  • a pL-level ultra-micro replenishing device comprising a pipetting needle, a motion controller, a glass microtube connector, a flow control device and a liquid conveying device;
  • the glass microtube connector is designed according to the principle of the connector and the principle of capillary phenomenon, and is composed of a communicating device, a capillary micro tube and a liquid overflow port; the axial center line of the pipetting needle is connected with the glass micro tube.
  • the axial centerline of the capillary tube portion in the device coincides;
  • the motion controller is fixedly connected with the pipetting needle to control the up and down movement of the pipetting needle in the glass microtube connector;
  • the flow control device is composed of a serially connected communication interface, an infusion hose, a flow rate regulator, a drip pot and a needle nozzle, and the communication interface is connected with the glass microtube communication device, and is connected to the liquid delivery device through the needle nozzle;
  • the liquid delivery device is coupled to the glass microtube connector by a flow control device to provide a liquid.
  • the bottom end of one end of the communicating device is connected to the capillary micro tube, and the upper part of the capillary micro tube extends into the inner cavity of the communicating body, and the lower half extends out of the communicating device; the liquid overflow port It is disposed on the bypass side of the glass microtube connector, and the liquid overflow port is located outside the communication bypass branch pipe.
  • the upper part of the capillary tube protrudes into the inner cavity of the connector, and the length of the capillary tube extends from 10 mm to 15 mm; the lower half extends out of the connector, and the length of the protrusion is 5 mm to 10 mm; the inner diameter of the capillary tube is 0.8- 1mm; the inner diameter of the main liquid storage chamber in the communicating device ranges from 25mm to 26mm, and the inner diameter of the bypass bypassing tube is 3-4mm.
  • liquid overflow heights are used for liquids with different viscosity properties, and the distance between the liquid liquid surface line and the upper end of the capillary microtube is controlled, and the liquid level is higher than the capillary micro tube. 1-2mm.
  • the pipetting needle is processed by ultra-fine precision electric discharge of a tungsten wire needle, and the tip size is determined according to the liquid distribution amount requirement.
  • the motion controller is composed of a linear servo motor, a servo driver, and a pipetting needle clamp; the pipetting needle clamp adopts a three-jaw chuck structure for clamping the pipetting needle.
  • the communicating device comprises a liquid main liquid storage chamber, a communication tube, a bypass rehydration tube, wherein the liquid main liquid storage chamber is used for storing the liquid delivered by the rehydration device, supplying the capillary micro tube liquid, and the communication tube is used for connecting the bypass rehydration liquid.
  • the tube and the liquid main reservoir chamber; the capillary microtubes are used to store the liquid in contact with the pipetting needle in the ultra-micro spotting device; the liquid overflow port is used to control the height of the liquid level line in the main liquid reservoir.
  • the flow control device can adjust the speed of rehydration and the amount of rehydration to meet the needs of liquids of different viscosities.
  • the flow control device consists of a communication interface, an infusion hose, a flow rate regulator, a drip pot, and a needle tip.
  • the communication interface is used for connecting the flow control device and the glass microtube connector bypass rehydration tube
  • the infusion hose is used for conveying the liquid
  • the flow rate regulator is used for adjusting the rehydration speed
  • the drip pot is used for observing the rehydration liquid flow rate and eliminating the infusion hose.
  • the bubble is used to connect the flow control device to the liquid delivery device.
  • the rehydration method for the pL-level ultra-micro rehydration device includes: the micro-rehydration is to clamp the pipetting needle through the pipetting needle clamp in the motion controller, and the linear servo motor drives the pipetting needle to move up and down in the micro-tube of the connector The micro-pressure difference between the inside and the outside of the micro-tube is generated, and the liquid in the communicating device is sucked into the micro-tube to replenish the pL-level liquid in the micro-tube.
  • control of the rehydration amount is to control the liquid amount of the micro-rehydration by moving the pipette needle up and down by changing the tip geometry of the pipetting needle and the up-and-down motion frequency, thereby satisfying the requirement of different rehydration amounts under the pL-level volume dimension, wherein
  • the up and down motion frequency is determined by the pulse frequency received by the motion controller.
  • the diameter of the infusion hose in the flow control device is controlled to adjust the infusion flow rate, thereby realizing the rehydration function of the liquid of different viscosities; in the above flow control device, through the drip pot Observe the drip frequency of the droplets and obtain the real-time rehydration flow rate.
  • FIG. 1 is a schematic view showing the structure of a pL-stage micro-continuous rehydration device according to an example of the present invention.
  • FIG. 2 is a schematic view showing the structure of a glass microtube connector of an example of the present invention.
  • Fig. 3 is a schematic diagram of a pL-level micro rehydration solution according to an example of the present invention.
  • FIG. 4 is a schematic diagram of a flow control device of an example of the present invention.
  • FIG. 5 is a schematic diagram of flow monitoring according to an example of the present invention.
  • Figure 6 is a schematic diagram of a flow regulating device of an example of the present invention.
  • 1-glass microtube connector 101-connector; 102-capillary microtube; 103-liquid overflow port; 2-pipetting needle motion controller; 3-pipetting needle; 4-first stent; 5-liquid; 6-flow control device; 601-connected interface; 602-infusion hose; 603-flow rate regulator; 60301-pulley linear guide; 60302-pulley; 604-drip pot; 605-needle; 7-liquid Conveying device; 701-storage tank; 702-pressure device; 8-second bracket; 9- tiny droplets.
  • a pL-level ultra-micro replenishing device suitable for an ultra-micro spotting platform includes a pipetting needle 3, a motion controller 2, a glass microtube connector 1, a flow control device 6, and a liquid delivery device 7. Five parts.
  • the glass microtube connector 1 includes a first bracket 4, a connector 101 mounted on the first bracket 4, and capillary microtubes 102 mounted at the bottom end of one end of the connector, and a liquid overflow port 103.
  • the micro-rehydration shown in FIG. 2 and FIG. 3 is a small pressure difference between the inside and the outside of the micro-tube generated during the up-and-down movement of the pipetting needle 3 in the capillary tube 102 by the motion controller 2, and the liquid in the communication device 101 is sucked.
  • the supplement of the pL grade liquid in the microtube is realized.
  • the liquid 5 in the communicating device 101 is replenished into the capillary microtubes 102 in the form of minute droplets 9, thereby achieving a continuous micro-replenishment of the liquid 5 in the capillary microtubes 102, thereby completing the ultra-micro spotting action.
  • the protrusion size of the capillary tube 102 in the connector 101 and the height of the liquid overflow can be adjusted to meet the requirements of different viscosity liquids.
  • the flow control device 6 includes a communication port 601, an infusion hose 602, a flow rate adjuster 603, a drip pot 604, and a needle 605, which are sequentially connected, and the communication interface 601 and the bypass device 101 are bypassed.
  • the pipe interfaces are connected.
  • the two ends of the communication interface are respectively connected to the infusion tube 602 and the bypass unit of the communication device 101, and the flow rate adjuster 603 is sleeved on the outer end of the infusion hose 602, and the two ends of the infusion hose 602 are respectively connected with the communication interface 601.
  • the drip pot Connected to the lower opening of the drip pot 604, the drip pot is connected to the needle 605 at the infusion hose 602, respectively.
  • the liquid level in the communicating device and the upper port of the capillary tube 102 are maintained at a distance of 1-2 mm to prevent the capillary microtube 102 from being unable to rely on the liquid in the adsorption tube to cause liquid due to the excessive amount of liquid 5 in the communicating device.
  • the problem of leaking is a problem of leaking.
  • the flow rate adjuster 603 adjusts the diameter of the infusion hose 602 by adjusting the upper and lower positions of the pulley 60302 in the linear guide rail 60301, thereby controlling the liquid flow rate.
  • the drip pot 604 in the flow control device 6 obtains the refilling speed by observing the drop frequency of the droplet, thereby adjusting the flow controller to maintain the liquid level line in the communicating device and the upper port of the capillary tube 102.
  • the distance is maintained at 1-2mm.
  • the liquid delivery device 7 is fixed by a second support 8, and the liquid delivery device 7 is connected to the glass microtube connector 1 through a flow communication device 6 end communication port 601, and the liquid delivery device 7 delivers liquid through the pressure device 702 to The flow rate control device 6 replenishes the glass microtube connector 1 with the liquid 5 through the flow rate control device 6.
  • the communicating device 101 comprises a liquid main liquid storage chamber, a communication tube, a bypass rehydration tube, wherein the liquid main liquid storage chamber is used for storing the liquid delivered by the rehydration device, and supplies the capillary micro tube 102 liquid, and the communication tube is used for connecting the bypass rehydration tube.
  • the liquid main liquid storage chamber; the capillary micro tube 102 is used for storing the liquid in contact with the pipetting needle 3 in the ultra-micro spotting device;
  • the liquid overflow port 103 is for controlling the height of the liquid level line of the liquid main liquid storage chamber.
  • the flow control device 6 can adjust the speed of rehydration and the amount of rehydration to meet the needs of different viscosity liquids.
  • the flow control device 6 is composed of a communication port 601, an infusion hose 602, a flow rate adjuster 603, a drip pot 604, and a needle tip 605.
  • the communication interface 601 is configured to connect the flow control device 6 with the glass microtube connector 1 to bypass the rehydration tube, the infusion hose 602 is used to transport the liquid 5, the flow rate adjuster 603 is used to adjust the rehydration speed, and the drip pot 604 is used to observe the rehydration solution.
  • the liquid flow rate and the elimination of air bubbles in the infusion hose are used to connect the flow control device 6 with the liquid delivery device 7.
  • the micro-rehydration is to clamp the pipetting needle 3 through the pipetting needle holder in the motion controller 2, and the linear servo motor drives the pipette inside and outside of the micro-tube during the up-and-down movement of the pipetting needle 3 in the communicating microtube.
  • a slight pressure difference causes the liquid in the communicating device to be sucked into the microtube to replenish the pL-level liquid in the microtube.
  • the control of the amount of fluid replacement is to control the liquid amount of the micro-rehydration by moving the pipette needle 3 up and down by changing the geometry of the tip end of the pipetting needle 3 and the frequency of the up-and-down motion, so as to meet the requirements of different rehydration amounts under the pL-level volume dimension, wherein
  • the frequency of the motion is determined by the frequency of the pulses received by the motion controller 2.
  • the diameter of the infusion hose 602 in the flow control device 6 is controlled to adjust the infusion flow rate, thereby realizing the rehydration function for the liquid of different viscosities; in the flow control device 6 described above, The drip frequency of the droplets in the drip pot 604 is observed to obtain a real-time rehydration flow rate.

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Abstract

一种pL级超微量补液装置及方法，补液装置包括移液针(3)，运动控制器(2)，玻璃微管连通器(1)，流量控制装置(6)，液体输送装置(7)五部分。其中，运动控制器(2)控制移液针(3)上下运动，玻璃微管连通器(1)依据连通器原理与毛细现象原理设计而成，流量控制装置(6)可以调节补液的速度和补液量，满足不同粘度液体的需要，液体输送装置(7)通过连通接口(601)将流量控制装置(6)与玻璃微管连通器(1)连接，为玻璃微管连通器(1)提供微量液体。pL级超微量补液方法是通过移液针(3)在毛细微管(102)中的上下运动过程中产生的毛细微管(102)内外的微量压力差，将连通器内(101)的液体吸入毛细微管(102)内，实现毛细微管(102)内pL级液体的补充。该装置用于各种粘度液体的pL级超微量连续补液且流量速度易于观测、调节。

Description

pL 级超微量补液装置及方法
技术领域
本发明涉及点样技术领域，特别是涉及一种用于微装配的 pL （皮升）级 超微量点样平台的补液装置及方法。
背景技术
点样技术的应用范围很广，从半导体封装工业、集成电路产业、SMT/PCB装配业到一般性工业的焊接、注涂和密封点样，点样技术都起着至关重要的作用。本发明在华南理工大学张勤提出的超微量点样方法的基础上提出了新式微量连续补液装置，解决了原有点样方法，无法连续补液的问题。超微量点样方法是采用移液针穿过玻璃微管内的液体时，移液针先端吸附的微小液滴实现超微量点样。其中点样液体的存储是位于玻璃微管当中，其原理是依靠毛细现象往玻璃微管当中填充液体。原有装置中，依靠毛细现象来实现液体存储玻璃微管的玻璃微管装置，受限于存储原理及自身结构的影响，毛细管无法存储大量液体，且无法实现对毛细管的实时微量连续补液。因此，原有超微量点样装置中无法实现点样动作的连续进行，需要停止点样动作，进行液体的补充。受限于此，原方法无法满足对超微量点样平台连续作业的要求，只适用于极少量，不连续的点样应用领域。本发明提出的方法，可以实现超微量点样装置的微量连续补液；在保留原有依靠毛细现象实现液体存储的玻璃微管装置基础上，依靠连通器原理，发明了一种实时补液装置。解决了原有超微量点样平台无法连续作业的问题。
发明内容
为了克服上述现有技术的不足，本发明在保留原有依靠毛细现象实现液体存储的玻璃微管装置基础上，依靠连通器原理，设计发明了pL级超微量补液装置及方法。
本发明的目的至少通过如下技术方案之一实现。
一种pL级超微量补液装置，包括移液针、运动控制器、玻璃微管连通器、流量控制装置和液体输送装置； 所述的玻璃微管连通器依据连通器原理与毛细现象原理设计而成，由连通器、毛细微管、液体溢流口组成；所述的移液针的轴向中心线与玻璃微管连通器中毛细微管部分轴向中心线重合；
所述的运动控制器与移液针固连，控制移液针在玻璃微管连通器中的上下移动；
所述流量控制装置由依次连接的连通接口、输液软管、流速调节器、滴壶和针嘴组成，连通接口与玻璃微管连通器连通，通过针嘴与液体输送装置连接；
所述液体输送装置通过流量控制装置与玻璃微管连通器连接，提供液体。
进一步地，所述的玻璃微管连通器中，连通器一端的底端连接毛细微管，且毛细微管上半部分伸入连通器内腔，下半部分伸出连通器；液体溢流口设置于玻璃微管连通器旁路一侧，液体溢流口位置位于连通器旁路支管外侧。
进一步地，毛细微管上半部分伸入连通器内腔，伸入的长度为10mm-15mm；下半部分伸出连通器，伸出的长度为5mm-10mm；毛细微管的内径范围0.8-1mm；连通器中主储液腔内径尺寸范围25mm-26mm，连通器旁路补液管内径尺寸范围3-4mm。
进一步地，所述的玻璃微管连通器中，针对不同粘度属性的液体，采用不同的液体溢流口高度，控制液体液面线与毛细微管上端的距离，液面高度高于毛细微管1-2mm。
进一步地，所述的移液针由钨丝针超微细精密放电加工而成，针尖尺寸根据液体分配量要求确定。
进一步地，所述的运动控制器由直线伺服电机、伺服驱动器、移液针夹具组成；移液针夹具采用三爪卡盘结构用以夹紧移液针。
进一步地，连通器包括液体主储液腔，连通管，旁路补液管，其中液体主储液腔，用于存储补液装置输送过来的液体，供给毛细微管液体，连通管用于连通旁路补液管与液体主储液腔；毛细微管用于储存超微量点样装置中与移液针接触的液体；液体溢流口用于控制液体主储液腔液面线的高度。
进一步地，流量控制装置可以调节补液的速度和补液量，满足不同粘度液体的需要。流量控制装置由连通接口，输液软管，流速调节器，滴壶，针嘴组成。其中连通接口用于连通流量控制装置与玻璃微管连通器旁路补液管，输液软管用于输送液体，流速调节器用于调节补液速度，滴壶用于观测补液液体流速和排除输液软管中的气泡，针嘴用于连通流量控制装置与液体输送装置。
适用于pL级超微量补液装置的补液方法，包括：微量补液是通过运动控制器中移液针夹具夹紧移液针，由直线伺服电机带动移液针在连通器微管中的上下运动过程中产生的微管内外的微量压力差，将连通器内的液体吸入微管内，实现微管内pL级液体的补充。
进一步地，补液量的控制是通过改变移液针的先端几何尺寸以及上下运动频率控制移液针上下运动一次微量补液的液体量，满足在pL级体积量纲下对不同补液量的要求，其中上下运动频率由运动控制器接收的脉冲频率决定。
进一步地，通过对流速调节装置中滑轮的旋转调节，控制流量控制装置中输液软管的管径来调节输液流速，实现对不同粘度液体的补液功能；上述的流量控制装置中，通过对滴壶中液滴的滴落频率的观测，获取实时的补液流速。
与现有技术相比，本发明的有益效果是：
（1） 实现了pL级超微量点样装置的实时补液功能。
（2） 实现了pL级超微量点样装置的连续不间断点样功能。
（3） 实现了pL级超微量点样装置的补液速度的监测功能。
（4） 实现了pL级超微量点样装置的补液速度调节的功能。
（5） 实现了pL级超微量点样装置对不同液体粘度的补液操作。
（6） 实现了压力式补液方法均匀出液的功能。
附图说明
图1为本发明实例的pL级微量连续补液装置的结构原理图。
图2为本发明实例的玻璃微管连通器的结构示意图。
图3为本发明实例的pL级微量补液原理图。
图4为本发明实例的流量控制装置的示意图。
图5为本发明实例的流量监测示意图。
图6为本发明实例的流量调节装置的原理图。
图中：1-玻璃微管连通器；101-连通器；102-毛细微管；103-液体溢流口；2-移液针运动控制器；3-移液针；4-第一支架；5-液体；6-流量控制装置；601-连通接口；602-输液软管；603-流速调节器；60301-滑轮直线导轨；60302-滑轮；604-滴壶；605-针嘴；7-液体输送装置；701-储液槽；702-压力装置；8-第二支架； 9-微小液滴。
具体实施方式
下面结合附图和具体实施例对本发明的发明目的作进一步详细地描述，实施例不能在此一一赘述，但本发明的实施方式并不因此限定于以下实施例。除非特别说明，本发明采用的材料和加工方法为本技术领域常规材料和加工方法。
如图1所示，一种适用于超微量点样平台的pL级超微量补液装置，包括移液针3，运动控制器2，玻璃微管连通器1，流量控制装置6，液体输送装置7五部分。
所述的玻璃微管连通器1包括第一支架4，安装在第一支架4上的连通器101，以及安装在连通器一端底端的毛细微管102，以及液体溢流口103。
如图2、图3所示微量补液是通过运动控制器2带动移液针3在毛细微管102中的上下运动过程中产生的微管内外的微量压力差，将连通器101内的液体吸入微管内，实现微管内pL级液体的补充。将连通器101内的液体5以微小液滴9的形式补充进入毛细微管102中，实现对毛细微管102中液体5的连续微量补充，进而完成超微量点样动作。不同玻璃微管连通器1中，毛细微管102在连通器101中的伸出尺寸以及液体溢流口高度尺寸可以调整，以满足不同粘度液体的需求。
如图4所示，所述的流量控制装置6包括由依次连接的连通接口601、输液软管602、流速调节器603、滴壶604和针嘴605组成，连通接口601与连通器101旁路管接口相连。所述的连通接口两端分别与输液软管602和连通器101旁路管接口连接，流速调节器603套在输液软管602外端，所述的输液软管602两端分别与连通接口601和滴壶604下通口连接，所述的滴壶分别于输液软管602与针嘴605连接。通过流量控制装置6，维持连通器内液面高度与毛细微管102上端端口距离维持1-2mm，防止因为连通器内液体5量过多而导致的毛细微管102无法依靠吸附管内液体导致液体下漏的问题。
如图5所示，所述的流量控制装置6中流速调节器603，通过调节滑轮60302在滑轮直线导轨60301中的上下位置，调节输液软管602的管径大小，进而控制液体流速。
如图6所示，所述的流量控制装置6中滴壶604，通过观测液滴的下落频率获取补液速度，进而调节流量控制器，维持连通器内液体液面线与毛细微管102上端端口距离维持1-2mm。
所述的液体输送装置7通过第二支架8固定，液体输送装置7通过流量控制装置6末端连通接口601与玻璃微管连通器1相连，所述的液体输送装置7通过压力装置702输送液体至流量控制装置6，通过流量控制装置6给玻璃微管连通器1补充液体5。
连通器101包括液体主储液腔，连通管，旁路补液管，其中液体主储液腔，用于存储补液装置输送过来的液体，供给毛细微管102液体，连通管用于连通旁路补液管与液体主储液腔；毛细微管102用于储存超微量点样装置中与移液针3接触的液体；液体溢流口103用于控制液体主储液腔液面线的高度。
流量控制装置6可以调节补液的速度和补液量，满足不同粘度液体的需要。流量控制装置6由连通接口601，输液软管602，流速调节器603，滴壶604，针嘴605组成。其中连通接口601用于连通流量控制装置6与玻璃微管连通器1旁路补液管，输液软管602用于输送液体5，流速调节器603用于调节补液速度，滴壶604用于观测补液液体流速和排除输液软管中的气泡，针嘴605用于连通流量控制装置6与液体输送装置7。
本实例中，微量补液是通过运动控制器2中移液针夹具夹紧移液针3，由直线伺服电机带动移液针3在连通器微管中的上下运动过程中产生的微管内外的微量压力差，将连通器内的液体吸入微管内，实现微管内pL级液体的补充。
补液量的控制是通过改变移液针3的先端几何尺寸以及上下运动频率控制移液针3上下运动一次微量补液的液体量，满足在pL级体积量纲下对不同补液量的要求，其中上下运动频率由运动控制器2接收的脉冲频率决定。
通过对流速调节装置603中滑轮60302的旋转调节，控制流量控制装置6中输液软管602的管径来调节输液流速，实现对不同粘度液体的补液功能；上述的流量控制装置6中，通过对滴壶604中液滴的滴落频率的观测，获取实时的补液流速。

Claims (8)

  1. pL级超微量补液装置，其特征在于包括移液针（3）、运动控制器（2）、玻璃微管连通器（1）、流量控制装置（6）和液体输送装置（7）； 所述的玻璃微管连通器（1）依据连通器原理与毛细现象原理设计而成，由连通器（101）、毛细微管（102）、液体溢流口（103）组成；所述的移液针（3）的轴向中心线与玻璃微管连通器（1）中毛细微管（102）部分轴向中心线重合；
    所述的运动控制器（2）与移液针（3）固连，控制移液针（3）在玻璃微管连通器（1）中的上下移动；
    所述流量控制装置（6）由依次连接的连通接口（601）、输液软管（602）、流速调节器（603）、滴壶（604）和针嘴（605）组成，连通接口（601）与玻璃微管连通器（1）连通，通过针嘴（605）与液体输送装置（7）连接；
    所述液体输送装置（7）通过流量控制装置（6）与玻璃微管连通器（1）连接，提供液体（5）。
  2. 根据权利要求1所述的pL级超微量补液装置，其特征在于：所述的玻璃微管连通器（1）中，连通器（101）一端的底端连接毛细微管（102），且毛细微管（102）上半部分伸入连通器（101）内腔，下半部分伸出连通器（101）；液体溢流口（103）设置于玻璃微管连通器（1）旁路一侧，液体溢流口（103）位置位于连通器（101）旁路支管外侧。
  3. 根据权利要求2所述的pL级超微量补液装置，其特征在于：毛细微管（102）上半部分伸入连通器（101）内腔，伸入的长度为10mm-15mm；下半部分伸出连通器（101），伸出的长度为5mm-10mm；毛细微管（102）的内径范围0.8-1mm；连通器（101）中主储液腔内径尺寸范围25mm-26mm，连通器（101）旁路补液管内径尺寸范围3-4mm。
  4. 根据权利要求2所述的pL级超微量补液装置，其特征在于：所述的玻璃微管连通器（1）中，针对不同粘度属性的液体，采用不同的液体溢流口（103）高度，控制液体液面线与毛细微管（102）上端的距离，液面高度高于毛细微管（102）1-2mm。
  5. 根据权利要求2所述的pL级超微量补液装置，其特征在于：所述的移液针（3）由钨丝针超微细精密放电加工而成，针尖尺寸根据液体分配量要求确定。
  6. 根据权利要求1所述的pL级超微量补液装置，其特征在于：所述的运动控制器（2）由直线伺服电机、伺服驱动器、移液针夹具组成；移液针夹具采用三爪卡盘结构用以夹紧移液针（3）。
  7. 利用权利要求1所述的pL级超微量补液装置的补液方法，其特征在于：微量补液是通过运动控制器（2）中移液针夹具夹紧移液针（3），由直线伺服电机带动移液针（3）在连通器微管中的上下运动过程中产生的微管内外的微量压力差，将连通器内的液体吸入微管内，实现微管内pL级液体的补充。
  8. 根据权利要求7所述的补液方法，其特征在于：补液量的控制是通过改变移液针（3）的先端几何尺寸以及上下运动频率控制移液针（3）上下运动一次微量补液的液体量，满足在pL级体积量纲下对不同补液量的要求，其中上下运动频率由运动控制器（2）接收的脉冲频率决定。
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