WO2018035457A1

WO2018035457A1 - Methods and compositions for treating canine conditions using recombinant self-complementary adeno-associated virus

Info

Publication number: WO2018035457A1
Application number: PCT/US2017/047607
Authority: WO
Inventors: Jeffrey S. Bartlett
Original assignee: Calimmune, Inc.
Priority date: 2016-08-19
Filing date: 2017-08-18
Publication date: 2018-02-22
Also published as: EP3500279A4; EP3500279A1; US20210283222A1

Abstract

Methods and compositions for treating symptoms of conditions such as but not limited to osteoarthritis and rheumatoid arthritis in canines. The methods may feature direct intraarticular injection of a recombinant self-complementary adeno-associated virus (sc-rAAV) with a vector adapted to express a modified IL-1Ra peptide. The methods of the present invention may express a therapeutically effective amount of the modified IL-1Ra peptide so as to ameliorating symptoms associated with the condition being treated.

Description

METHODS AND COMPOSITIONS FOR TREATING CANINE CONDITIONS USING RECOMBINANT SELF-COMPLEMENTARY ADENO-ASSOCIATED VIRUS

CROSS REFERENCE

[0001] This application claims priority to U.S. Provisional Patent Application No. 62/377,346 filed August 19, 2016, the specification(s) of which is/are incorporated herein in their entirety by reference.

REFERENCE TO SEQUENCE LISTING

[0002] Applicant asserts that the information recorded in the form of an Annex C/ST.25 text file submitted under Rule 13fer.1 (a), entitled CALIM __16__04_PCT_Sequence__Lisfing_ST25.txf, is identical to that forming part of the international application as filed. The content of the sequence listing is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

[0003] The present invention relates to gene therapy and compositions for gene therapy, more particularly to recombinant self-complementary adeno-associated virus (sc-rAAV) and methods of treating conditions or symptoms of conditions using sc-rAAV.

BACKGROUND OF THE INVENTION

[0004] IL-1 is a powerful mediator of both chondrocytic chondrolysis and suppression of matrix synthesis by chondrocytes. Together, these two processes are highly destructive to cartilage. IL-1 has also been shown to inhibit chondrogenesis but at the same time promote certain aspects of the osteogenic differentiation that could help account for the formation of osteophytes and sclerosis of sub-chondrai bone. In studying cartilage recovered from human joints with OA, the production of IL-1 by chondrocytes was found to be highly elevated and sustained in an autocrine fashion. Moreover, the cells did not produce IL-1 Ra. This suggests enhanced autocrine and paracrine activation of chondrocytes by IL-1 in the absence of its major physiological inhibitor during OA. Enhanced responsiveness of chondrocytes to IL-1 in OA was also indicated by increased expression of the type I IL-1 receptor, the signaling receptor, on OA chondrocytes. The local production and consumption of IL-1 by chondrocytes may help explain why concentrations of IL-1 in synovial fluid tend to be low, even in OA. Also, genetic analyses have identified single nucleotide polymorphisms (SNPs) in the human gene encoding !L-1 Ra (IL1 RN) and regulatory elements that correlate with the incidence and severity of certain types of OA.

[0005] Targeted drug delivery is a major problem for the intra-articular treatment of joint diseases. Molecules of all sizes, as well as particles, are rapidly removed from joints via the lymphatics, subsynovial capillaries, or both. This makes it difficult to achieve sustained, therapeutic doses of anti-OA drugs in joints. To address this, small molecules can be delivered systemicaliy, but proteins are difficult to deliver in this fashion because of size-dependent constraints in crossing the fenestrated endothelium of the synovial capillaries. Moreover, systemic delivery exposes non- target sites to high doses of the therapeutic, leading to unwanted side-effects. The rapid egress of proteins from joints, with half-lives typically of a few hours, makes infra-articular delivery potentially ineffective. As an example, recombinant !L-1 Ra (Kineret, Amgen Biologicais) is delivered by daily subcutaneous injection in effort to treat symptoms of RA. However, daily delivery fails to maintain therapeutic serum levels of !L-1 Ra between injections (Evans et ai., 1996, Human Gene Therapy, 7:1261-1290; Evans et ai., 2005, PNAS 102 (24): 8698-8703). Some studies have used ex vivo gene transfer for introducing IL-1 Ra to treat OA. However, these approaches are laborious and have not seemed to provide long-term gene expression (Frisbie et al., 2002, Gene Therapy 9(1 ): 12-20). Also, several studies describe the use of a dual variable domain-immunogiobulin (DVD-lg) targeting !L- 1 alpha and !L-1 beta (e.g., ABT-981 ) for treating osteoarthritis (Kamath et al., 201 1 , Osteoarthritis and Cartilage 19S1 :S64; Wang et al., 2015, Osteoarthritis and Cartilage 23:A398-399; Wang et al., 2014, Osteoarthritis and cartilage 22:S462- S463; Lacy et ai., 2015, mAbs 7(3):605-619; Wu et ai., 2009, mAbs 1 (4):339-347; Wang et al., 2014, Scientific Abstracts SAT0448 pg. 756; Goss et al., 2014, Scientific Abstracts SAT0447 pg. 755-756; US 2015/0050238; Wang et al., 2014 ACR/ARHP Annual Meeting Abstract Number 2237; Wang et al., 2015 ACR/ARHP Annual Meeting Abstract Number 318). However, these peptides require repeated systemic introduction (e.g., 4 doses every 2 weeks or 3 doses every 4 weeks, e.g., by subcutaneous injection or intravenous infusion) because of the relatively short half-life (Wang et al., 2015, Osteoarthritis and Cartilage 23:A398-399; Wang et al., 2014, Osteoarthritis and cartilage 22:S462-S463; Evans et al., 2005, PNAS 102 (24): 8698-8703).

[0006] The present invention features methods and compositions for delivering a therapeutic gene product (e.g., IL-1 Ra) in a sustained manner to a location of interest, e.g., joints, in canine animals, e.g., members of the family Canidae, e.g., Canis famsliaris, Canss lupus, etc. The present invention also features methods and compositions for treating symptoms of conditions such as but not limited to osteoarthritis and rheumatoid arthritis. The present invention also features methods and compositions for providing a canine a therapeutically effective amount of a therapeutic gene product (e.g., IL-1 Ra). The methods and compositions may feature a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein the sc-rAAV comprises an engineered capsid and a vector (e.g., a sc-rAAV vector) packaged within the capsid. The vector may comprise a transgene (e.g., a nucleotide sequence encoding a protein of interest, e.g., a therapeutic gene product, e.g., !L-1 Ra or a codon modified version thereof) operably linked to a promoter (e.g., a constitutive promoter). The therapeutic gene product may be delivered to a location of interest, e.g., a joint. For example, for treating osteoarthritis, the sc-rAAV may be introduced into cells (e.g., chondrocytes, synoviocytes, etc.) in a joint via direct intraarticular injection. The present invention is not limited to the aforementioned conditions, nor the location of interest (e.g., joint).

[0007] It is noted that Goodrich et ai. (Molecular Therapy-Nucleic Acids, 2013, 2:e70) generally discloses a method of treating osteoarthritis using scAAV-deiivered IL-1 Ra. However, Goodrich et al. does not specifically identify or enable any particular IL-1 Ra sequence, e.g., an IL-1 Ra sequence according to the present invention, !n particular, the field of gene therapy is an unpredictable area wherein one cannot assume that any particular gene sequence for a protein of interest will be efficiently expressed.

SUMMARY OF THE INVENTION

[0008] The present invention features a recombinant self-complementary adeno- associated virus (sc-rAAV). Sn some embodiments, the sc-rAAV comprises an engineered AAV capsid and a vector packaged within the capsid, wherein the vector comprises a modified !L-1 Ra gene operably linked to a promoter and the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2. In some embodiments, the promoter comprises a CI promoter. In some embodiments, the engineered capsid comprises at least a portion of serotype AAV2 and at least a portion of serotype AAV6. In some embodiments, the engineered capsid comprises at least a portion of serotype AAV1 , AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV1 1 , or a combination thereof. In some embodiments, the vector further comprises SV40 and bovine growth hormone (bGH) poiyadenylation sequences. In some embodiments, the vector further comprises SV40 splice donor (SD) and splice acceptor (SA) sites. In some embodiments, the vector comprises sc-rAAV2,5Hu-!L- 1 Ra. In some embodiments, the sc-rAAV is part of a composition.

[0009] In some embodiments, the sc-rAAV comprises an engineered AAV capsid and a vector packaged within the capsid, wherein the vector comprises a modified IL-1 Ra gene operabiy linked to a promoter and the modified !L-1 Ra gene encodes IL-1 Ra protein according to SEQ ID NO: 5.

[0010] The present invention features a method of providing a canine in need thereof (e.g., a canine diagnosed with or at risk for osteoarthritis or rheumatoid arthritis) a therapeutically effective amount of interieukin-1 receptor agonist (IL-1 Ra) peptide. In some embodiments, the method comprises introducing into a location of interest (e.g., via intraarticular injection) a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV) according to the present invention. The sc-rAAV transduces the vector into ceils in the location of interest, wherein the modified IL-1 Ra gene is expressed so as to provide the canine with the therapeutically effective amount of said IL-1 Ra peptide.

[0011] The present invention also features a method of ameliorating symptoms of osteoarthritis or rheumatoid arthritis in a canine. In some embodiments, the method comprises introducing into a location of interest (e.g., via direct intraarticular injection) a composition comprising a recombinant self-complementary adeno- associated virus (sc-rAAV) according to the present invention. The sc-rAAV transduces the vector into cells in the location of interest, wherein the modified IL- 1 Ra gene is expressed so as to provide the canine with an amount of IL-1 Ra peptide effective for ameliorating symptoms associated with osteoarthritis or rheumatoid arthritis.

[0012] The present invention also features a method of repairing cartilage in a canine in need thereof (e.g., a canine diagnosed with or at risk for developing osteoarthritis or rheumatoid arthritis), !n some embodiments, the method comprises introducing into a location of cartilage (e.g., via direct intraarticular injection) a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV) according to the present invention. The sc-rAAV transduces the vector info cells in the location of cartilage, wherein the modified !L-1 Ra gene is expressed so as to provide the canine with IL-1 Ra peptide effective for repairing cartilage.

[0013] The present invention also features a method of providing interieukin-1 receptor agonist (lL~1 Ra) peptide to an area of inflammation. In some embodiments, the method comprises introducing into a location of inflammation (e.g., via intraarticular injection) a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV) according to the present invention. The sc-rAAV transduces the vector into cells in the location of inflammation, wherein the modified !L-1 Ra gene is expressed so as to provide the ceils in the location of inflammation a therapeutically effective amount of IL-1 Ra peptide effective for reducing inflammation.

[0014] In some embodiments, the location of interest is a joint, synovium, subsynovium, joint capsule, tendon, ligament, cartilage, or peri-articular muscle of the canine. In some embodiments, the celis are chondrocytes, synoviocytes, or a combination thereof. 0015J In some embodiments, the method is performed a second time at a time point after a time when the method is performed first. In some embodiments, the time point is at least 3 months. In some embodiments, the method further comprises co-introducing a secondary therapy (e.g., a glucocorticoid, hyaluronan, platelet-rich plasma, recombinant, canine IL-1 Ra, or a combination thereof) to the location of interest in combination with the composition. 0016J The present invention also features a method of delivering IL-1 Ra peptide to a chondrocyte or synoviocyte. In some embodiments, the method comprises contacting the chondrocyte or synoviocyte with a recombinant self-complementary adeno-associated virus (sc~rAAV) according to the present invention, e.g., an engineered adeno-associated virus (AAV) capsid comprising at least a portion of serotype 2 and at least a portion of serotype 6 and a vector packaged within the capsid, wherein the vector comprises a modified !L-1 Ra gene operably linked to a CMV promoter and the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2. The sc-rAAV transduces the vector into the chondrocyte or synoviocyte and the modified IL-1 Ra gene is expressed to as to provide IL-1 Ra peptide to the chondrocyte or synoviocyte.

[0017] For the aforementioned methods and compositions (e.g., a method of providing a canine in need thereof a therapeutically effective amount of interleukin-1 receptor agonist (IL-1 Ra) peptide, a method of ameliorating symptoms of osteoarthritis or rheumatoid arthritis in a canine, a method of delivering IL-1 Ra peptide to a chondrocyte or synoviocyte, a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), a recombinant self- complementary adeno-associated virus (sc-rAAV) vector comprising a modified IL- 1 Ra gene operably linked to a CMV promoter, a method of repairing cartilage in a canine in need thereof, a method of providing interleukin-1 receptor agonist (!L-1 Ra) peptide to an area of inflammation, etc.), the modified IL-1 Ra gene may be at least 95% identical SEQ ID NO: 2 and encode IL-1 Ra according to SEQ SD NO: 8 or SEQ ID NO: 9.

[0018] Any feature or combination of features described herein are included within the scope of the present invention provided that the features included in any such combination are not mutually inconsistent as will be apparent from the context, this specification, and the knowledge of one of ordinary skill in the art. Additional advantages and aspects of the present invention are apparent in the following detailed description and claims.

TERMS

[0019] Unless otherwise explained, ail technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which a disclosed invention belongs. The singular terms "a," "an," and "the" include plural referents unless context clearly indicates otherwise. Similarly, the word "or" is intended to include "and" unless the context clearly indicates otherwise. "Comprising" means "including." Hence "comprising A or B" means "including A" or "including B" or "including A and B."

[00201 Suitable methods and materials for the practice and/or testing of embodiments of the disclosure are described below. Such methods and materials are illustrative only and are not intended to be limiting. Other methods and materials similar or equivalent to those described herein can be used. For example, conventional methods well known in the art to which the disclosure pertains are described in various general and more specific references, including, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, 1989; Sambrook et al., Molecular Cloning: A Laboratory Manual, 3d ed., Cold Spring Harbor Press, 2001 ; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing Associates, 1992 (and Supplements to 2000); Ausubel et al., Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 4th ed., Wiley & Sons, 1999; Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1990; and Harlow and Lane, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1999, the disclosures of which are incorporated in their entirety herein by reference.

[0021] Ail publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety.

[0022J Although methods and materials similar or equivalent to those described herein can be used to practice or test the disclosed technology, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting.

[0023] In order to facilitate review of the various embodiments of the disclosure, the following explanations of specific terms are provided: [0024] Adeno-assodated virus (AAV), Recombina t AAV (rAAV), a d Recombinant Self-Complementary AAV (sc-rAAV): AAV is a small virus (20 nm) in the family Parvoviridae. AAV is not known to cause disease. AAV has recently been used to gene therapy for a variety of reasons including that it has been shown to have low immunogenicity, the ability to effectively transduce non-dividing cells, and the ability to infect a variety of cell and tissue types. Recombinant AAV (rAAV) does not contain native viral coding sequences. Recombinant AAV DNA is packaged into the viral capsid as a single stranded molecule about 4600 nucleotides in length. Following infection of the ceil by the virus, the molecular machinery of the cell converts the single DNA strand into a double- stranded form. Only the double stranded DNA form is useful to the proteins of the ceil that transcribe the contained gene or genes into RNA. Self-complementary AAV (sc-rAAV) is an engineered form of rAAV that can form an intra-moiecular double stranded DNA template. Thus, upon infection, the two complementary halves of sc-rAAV will associate to form one double stranded DNA unit that is ready for immediate replication and synthesis.

[0025] Expression: The translation of a nucleic acid sequence into a protein. Proteins may be expressed and remain intracellular, become a component of the ceil surface membrane, or be secreted info the extracellular matrix or medium,

[00261 Operably linked: A first nucleic acid sequence is operably linked with a second nucleic acid sequence when the first nucleic acid sequence is placed in a functional relationship with the second nucleic acid sequence. For instance, a promoter is operably linked to a coding sequence if the promoter affects the transcription or expression of the coding sequence.

[0027J Pharmaceutically acceptable vehicles: Pharmaceutically acceptable carriers (vehicles), e.g., solutions, may be conventional but are not limited to conventional vehicles. For example, E. W. Martin, Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, PA, 15th Edition (1975) and D. B. Troy, ed. Remington: The Science and Practice of Pharmacy, Lippincott Williams & Wilkins, Baltimore MD and Philadelphia, PA, 21^st Edition (2006) describe compositions and formulations suitable for pharmaceutical delivery of one or more therapeutic compounds or molecules. In general, the nature of the carrier will depend on the particular mode of administration being employed. In addition to biologically-neutral carriers, pharmaceutical compositions administered may contain minor amounts of non- toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like, for example sodium acetate or sorbitan monoiaurate,

[00281 Preventing, treating, managing, or ameliorating a condition: "Preventing" a disease may refer to inhibiting the full development of a condition. "Treating" may refer to a therapeutic intervention that ameliorates a sign or symptom of a disease or pathological condition after it has begun to develop. "Managing" may refer to a therapeutic intervention that does not allow the signs or symptoms of a disease or condition to worsen. "Ameliorating" may refer to the reduction in the number or severity of signs or symptoms of a disease or condition.

[0029] Sequence identity: The identity (or similarity) between two or more nucleic acid sequences is expressed in terms of the identity or similarity between the sequences. Sequence identity can be measured in terms of percentage identity; the higher the percentage, the more identical the sequences are. Sequence similarity can be measured in terms of percentage similarity (which takes into account conservative amino acid substitutions); the higher the percentage, the more similar the sequences are. Methods of alignment of sequences for comparison are well known in the art. Various programs and alignment algorithms are described in: Smith & Waterman, Adv. Appl. Math. 2:482, 1981 ; Needleman & Wunsch, J. Mol. Biol. 48:443, 1970; Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444, 1988; Higgins & Sharp, Gene, 73:237-44, 1988; Higgins & Sharp, CABiOS 5:151-3, 1989; Corpet et ai., Nuc. Acids Res. 18:10881-90, 1988; Huang et ai. Computer Appls. in the Biosciences 8, 155-65, 1992; and Pearson et a!., Meth. Mol. Bio. 24:307-31 , 1994. Altschul et ai, J. Mol. Biol. 215:403-10, 1990, presents a detailed consideration of sequence alignment methods and homology calculations. The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul et ai, J. Mol. Biol. 215:403-10, 1990) is available from several sources, including the National Center for Biotechnology (NCBI, National Library of Medicine, Building 38A, Room 8N805, Bethesda, MD 20894) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. Additional information can be found at the NCBI web site. BLASTN may be used to compare nucleic acid sequences, while BLASTP may be used to compare amino acid sequences, !f the two compared sequences share homology, then the designated output file will present those regions of homology as aligned sequences. If the two compared sequences do not share homology, then the designated output file will not present aligned sequences. The BLAST-iike alignment tool (BLAT) may also be used to compare nucleic acid sequences (Kent, Genome Res. 12:656-684, 2002). BLAT is available from several sources, including Kent Informatics (Santa Cruz, CA) and on the Internet (genome.ucsc.edu). Once aligned, the number of matches is determined by counting the number of positions where an identical nucleotide or amino acid residue is presented in both sequences. The percent sequence identity is determined by dividing the number of matches either by the length of the sequence set forth in the identified sequence, or by an articulated length (such as 100 consecutive nucleotides or amino acid residues from a sequence set forth in an identified sequence), followed by multiplying the resulting value by 100. For example, a nucleic acid sequence that has 1 166 matches when aligned with a test sequence having 1554 nucleotides is 75.0 percent identical to the test sequence (1 166÷1554*100=75.0). The percent sequence identity value is rounded to the nearest tenth.

[0030] Therapeutically effective amount: A quantity of a specified agent sufficient to achieve a desired effect in a subject being treated with that agent. Such agents may include !L-1 Ra, For example, a therapeutically effective amount of !L-1 Ra may be an amount sufficient to prevent, treat, or ameliorate symptoms of osteoarthritis or rheumatoid arthritis. The therapeutically effective amount of an agent useful for preventing, ameliorating, and/or treating a subject will be dependent on the subject being treated, the type and severity of the affliction, and the manner of administration of the therapeutic composition.

[0031] Transduced: A transduced cell is a cell into which a nucleic acid molecule has been introduced by molecular biology techniques. As used herein, the term transduction encompasses ail techniques by which a nucleic acid molecule might be introduced into such a cell, including transfection with viruses or viral vectors, transformation with plasmid vectors, and introduction of naked DNA by e!ectroporation, lipofection, and particle gun acceleration. Such cells are sometimes called transformed cells.

[0032] Vector: A nucleic acid molecule as introduced into a host ceil, thereby producing a transformed host cell. A vector may include nucleic acid sequences that permit it to replicate in a host ceil, such as an origin of replication. A vector may lack the nucleic acid sequences that permit it to replicate in a host cell. A vector may also include a gene of interest, one or more selectable marker genes, other genetic elements known in the art, or any other appropriate insert.

DETAILED DESCRIPTION OF THE INVENTION

[0033] The present invention features methods and compositions for delivering a therapeutic gene product (e.g., IL-1 Ra) in a sustained manner to a location of interest, e.g., a joint. The present invention also features methods and compositions for treating symptoms of conditions such as but not limited to osteoarthritis or rheumatoid arthritis. The present invention also features methods and compositions for providing an individual (e.g., a canine) a therapeutically effective amount of a therapeutic gene product (e.g., IL-1 Ra). The methods and compositions may feature a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein the sc-rAAV comprises an engineered capsid and a vector (an sc-rAAV vector) packaged within the capsid. The vector may comprise a transgene (e.g., a

nucleotide sequence encoding a protein of interest, e.g., a therapeutic gene product, e.g., !L-1 Ra or a modified version thereof) operably linked to a promoter (e.g., a constitutive promoter).

[0034] As previously discussed, the present invention features compositions comprising a recombinant self-complementary adeno-associated virus (sc-rAAVs) vector. SEQ ID NO: 1 and SEQ ID NO: 2 show sequences for Canis familiaris IL- 1 Ra. SEQ ID NO: 3 and SEQ ID NO: 4 show modified versions of IL-1 Ra. The sc~ rAAV vector of the present invention comprises a modified IL-1 Ra gene. The present invention is not limited to the modified sequences of SEQ ID NO: 3 and SEQ ID NO: 4.

[0035] The sc-rAAV vectors comprise a nucleic acid that encodes a modified IL-1 Ra gene. In some embodiments, the nucleic acid is at least 90% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In some embodiments, the nucleic acid is at least 92% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO:

3, or SEQ !D NO: 4. In some embodiments, the nucleic acid is at least 94% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In some embodiments, the nucleic acid is at least 95% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, In some embodiments, the nucleic acid is at least 96% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO:

4. In some embodiments, the nucleic acid is at least 97% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In some embodiments, the nucleic acid is at least 98% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. In some embodiments, the nucleic acid is at least 99% identical to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. Non-limiting examples of such nucleic acid sequences can be found in Table 1 below, For example, SEQ ID NO: 5 is a sequence for a modified IL-1 Ra that is about 99% identical to SEQ ID NO: 2; SEQ ID NO: 8 is a sequence for a modified IL-1 Ra that is about 98% identical to SEQ ID NO: 2; and SEQ ID NO: 7 is a sequence for a modified IL-1 Ra that is about 95% identical to SEQ ID NO: 2 (note that the bold letters in Table 1 are nucleotide substitutions as compared to SEQ ID NO: 2, and the codon underlined).

Table 1

ggcc gtgtc aagtctggag atgagaccag gctccagctg gaggccgtta acatcactga cctgagtaag aacaaggatc aagacaagcg ctttaccttc atcctctcag acagtggccc taccaccagc tttgagtctg ctgcctgccc tggctggttc ctctgcacag cactggaggc cgaccggcct gtcagcctca ccaacagacc agaagaggcc atgatgg ca ctaagttcta cttccagaag gaataatagt gtgtccattc cgtgcttccc ccccactccc aacacatcaa tgactccaga gatgcctctc cattctgcct ggggtctcct ggctgtggtg gaggctctga ggagcagcct cggtggggtg gaccctcaga aggatgtatg agagccctgg taacgggacc ctgcctccag cctcc cagc tagccaacct caatgctgcc accacagtgg tctttctaaa gtgcacctct agctgcagca ctgctccagg cctttcaggg ctgcctctgc cttctggatt aaagccaggc tgcttggcca gcctggcccc ctgctctcct ctccgtaact ccttgctctc ctcccttgcc ccatgtccat gtcctgga c cctcctgccc ctttgctggc ctcccaaacc ttgtgttttg caaaccgaaa aaaaaaaaaa aacgatccgg tacctctaga tcagaa

Modified Canis familiaris ATGGAGACCTGCAGGTGCCCCCTGAGCTACCTGATCAGCTTCCTGCTG

TTCCTGAGCCACAGCGAGACCGCC GCAGGCCCCTGGGCAAGAGGCCC IL-1 Ra TGCAGGATGCAGGCCTTCAGGATCTGGGACGTGAACCAGAAGACCTTC

TACCTGAGGAACAACCAGCTGGTGGCCGGCTACCTGCAGGGCAGCAAC ACCAAGCTGGAGGAGAAGCTGGACGTGGTGCCCGTGGAGCCCCACGCC GTGTTCCTGGGCATCCACGGCGGCAAGCTGTGCCTGGCCTGCGTGAAG AGCGGCGACGAGACCAGGCTGCAGCTGGAGGCCGTGAACATCACCGAC CTGAGCAAGAACAAGGACCAGGACAAGAGGTTCACCTTCATCCTGAGC GACAGCGGCCCCACCACCAGCTTCGAGAGCGCCGCCTGCCCCGGCTGG TTCCTGTGCACCGCCCTGGAGGCCGACAGGCTGGTGAGCCTGACCAAC AGGCCCGAGGAGGCCATGATGGTGACCAAGTTCTACTTCCAGAAGGAG

Modified Canis familiaris ATGGAGACC GCCGCTGCCCCCTGAGCTACCTGATCAGCTTCC GCTG

TTCCTGAGCCACAGCGAGACCGCCTGCCGCCCCCTGGGCAAGCGCCCC IL-1 Ra TGCCGCATGCAGGCCTTCCGCATC GGGACGTGAACCAGAAGACCTTC

TACCTGCGCAACAACCAGCTGGTGGCCGGCTACCTGCA.GGGCAGCAAC ACCAAGCTGGAGGAGAAGCTGGACGTGGTGCCCGTGGAGCCCCACGCC GTGTTCCTGGGCATCCACGGCGGCAAGCTGTGCCTGGCCTGCGTGAAG AGCGGCGACGAGACCCGCCTGCAGCTGGAGGCCGTGAACATCACCGAC CTGAGCAAGAACAAGGACCAGGACAAGCGC TCACCTTCATCCTGAGC GACAGCGGCCCCACCACCAGCTTCGAGAGCGCCGCCTGCCCCGGCTGG TTCCTGTGCACCGCCCTGGAGGCCGACCGCCTGGTGAGCCTGACCAAC CGCCCCGAGGAGGCCATGATGGTGACCAAGTTCTACTTCCAGAAGGAG

Modified !L-1 Ra insert ATGGAGACCTGCA.GGTGCCCACTGAGTTACCTAATCAGTTTCCTCCTG

TTCCTGAGCCACAGCGAGACCGCCTGCAGGCCCCTGGGCAAGAGGCCC

(99% identical to SEQ ID TGCAGGATGCAGGCCTTCAGGATCTGGGACGTGAACCAGAAGACCTTC

TACCTGAGGAACAACCAGCTGGTGGCCGGCTACCTGCAGGGCAGCAAC NO: 2; bold letters are ACCAAGC GGAGGAGAAGCTGGACGTGGTGCCCGTGGAGCCCCACGCC nucleotide substitutions GTGTTCC GGGCATCCACGGCGGCAAGCTGTGCCTGGCCTGCGTGAAG

AGCGGCGACGAGACCAGGCTGCAGCTGGAGGCCGTGAACATCACCGAC

within a codon {codon is CTGAGCAAGAACAAGGACCAGGACAAGAGGTTCACCTTCATCCTGAGC

GACAGCGGCCCCACCACCAGCTTCGAGAGCGCCGCCTGCCCCGGCTGG

underlined)) TTCCTGTGCACCGCCCTGGAGGCCGACAGGCTGGTGAGCCTGACCAAC

AGGCCCGAGGAGGCCATGATGGTGACCAAGTTCTACTTCCAGAAGGAG

Modified IL-1 Ra insert ATGGAGACCTGCAGGTGCCCACTGAGTTACCTAATCAGTTTCCTCCTG

TTCCTGAGCCACAGCGAGACCGCCTGCAGGCCCCTGGGCAAGAGGCCC

{98% identical to SEQ ID TGCAGGATGCAGGCCTTCAGGATCTGGGACGTGAACCAGAAGACCTTC

TACCTGAGGAACAACCAGCTGGTGGCCGGCTACCTGCAGGGCAGCAAC NO: 2; bold letters are ACCAAGCTGGAGGAGAAGCTGGACGTGGTGCCCGTGGAGCCCCACGCC nucleotide substitutions GTGTTCCTGGGCATCCACGGCGGCAAGCTGTGCCTGGCCTGCGTGAAG

AGCGGCGACGAGACCAGGCTGCAGCTGGAGGCCGTGAACATCACCGAC

within a codon (codon is CTGAGCAAGAACAAGGACCAGGACAAGAGGTTCACCTTCATCCTGAGC

GACAGCGGCCCCACCACCAGCTTCGAGAGCGCCGCCTGCCCCGGCTGG

underlined)) TTCCTGTGCACCGCCCTGGAGGCCGACAGGCTGGTGAGCCTGACCAAC

AGGCCCGAGGAGGCAATGATGGTAACCAAATTCTATTTCCATAAGGAA I

Modified IL~1 Ra insert ATGGAGACCTGCAGGTGCCCACTGAGTTACCTAATCAGTTTCCTCCTG

TTCCTGAGCCACAGCGAGACCGCCTGCAGGCCCCTGGGCAAGAGGCCC

(95% identical to SEQ ID TGTAGGATGCAGGCCTTCAGGATCTGGGATGTGAACCAGAAGACCTTT

TACCTGAGGAATAACCAGCTGGTAGCCGGCTACCTGCAGGGGAGCAAC NO: 2; bold letters are

ACCAAGCTGGAGGAGAAGCTGGACGTGGTGCCCGTGGAGCCCCACGCC

nucleotide substitutions GTGTTTCTGGGCA.TCCACGGAGGCAAGCTGTGCCTAGCCTGCGTGAA.G

AGCGGCGACGAGACCAGGCTGCAGCTGGAGGCGGTGAACATCACCGAC CTGAGCAAGAACAAAGACCAGGACAAGAGGTTCACCTTCATCCTAAGC within a codon (codon is GACAGCGGCCCCACCACCAGCTTCGAAAGCGCCGCTTGCCCCGGCTGG

TTCCTCTGCACCGCCC GGAGGCCGACAGGCTGGTGAGCCTGACCAAC

underlined)) AGGCCCGAGGAGGCAATGATGGTAACCAAATTC ATTTCCATAAGGAA

[0036] In some embodimen^t ts, the !L-1 Ra peptide encoded by the !L-1 Ra insert comprises IL-1 Ra (see SEQ ID NO: 8, SEQ SD NO: 9 in Table 2 below).

Table 2

0037J The transgene (e.g., nucleotide sequence encoding protein of interest) is operably linked to a promoter. In some embodiments, the promoter comprises the cytomegalovirus (CMV) promoter. The present invention is not limited to the CMV promoter and may feature any appropriate promoter or portions of various promoters. Examples of promoters include CMV promoter, hybrid CMV promoter, CAG promoter, human beta-actin promoter, hybrid beta-actin promoter, EF1 promoter, U1 a promoter, U1 b promoter, a Tet-inducible promoter, a VP16-LexA promoter, chicken beta-actin (CBA) promoter, human elongation factor-1 alpha promoter, simian virus 40 (SV40) promoter, and herpes simplex virus thymidine kinase promoter. In some embodiments, the promoter comprises a hybrid promoter. As an example, Table 3 shows an IL-1 beta/IL~6 hybrid promoter (see also van de Loo et al., 2004, Gene Therapy 1 1 :581-590). The present invention is also not limited to the hybrid promoter shown in Table 3.

IL-1 beta/ atccaagag ggagaagaag cccattggag atgaigccai aaaggaagtg

gaagcgaiat gataaaaatc atagtgccca ttcccaaata atcccagaag

IL-8 hybrid cagaagggaa aggagagaaa taiccacaaa gacaggtgtg ggtacacaea promoter acatttttca tactttaaga tcccagagga ctcatggaaa tgaiacaaga

aaatgactca taagaacaaa tattaggaag ccagtgccaa gaatgagatg ggaaattggg gaaaatgttg ggggcagatt gcttagttct gttctaagca agagggtgaa caaggaagga acagctcact acaaagaaca gacatcactg catgtacaca caataatata agaactaacc catgattatt ttgcttgtct tcttgttcaa aatgattgaa gaccaatgag atgagatcaa ccitgaiaac tggctggcit

cggcatgatt agacacaaga tggtatcagg gcacttgctg ctttgaataa tgtcagtctc ctgtcttgga agaatgacct gacagggtaa agaggaactt gcagctgaga aaggctttag tgactcaaga gctgaataat tccccaaaag ctggagcatc ctggcatftc cagctcccca tctctgcttg ttccacttcc ttggggctac atcaccatct acatcatcat cactcticca ciccctccct tagtgccaac tatgtttata gcgagatatt ttctgctcat tggggatcgg aaggaagtgc tgtggcctga gcggtctcct tgggaagaca ggaictgata catacgtigc acaacctatt tgacataaga ggtttcactt cctgagatgg atgggatggi: agcagatttg ggiceaggft acagggccag gatgagacat ggcagaactg tggagactgt tacgtcaggg ggcattgccc catggcteea aaatttccct cgagc

ctctggccc caccctcacc ctccaacaaa gatttatcaa atgtgggatt ttcccatgac tctcaatatt agagtctcaa cccccaataa atataggact ggagatgtct gaggcieatt ctgcccicga gcccaccggg aacgaaagag aagetetatc icccctccag gagcccagct atgaactcct tc

[0038] In some embodiments, the sorAAV vector is packaged within a capsid. In some embodiments, the capsid comprises at least a portion of AAV serotype 1 (AAV1 ), AAV serotype 2, (AAV2), AAV serotype 3, (AAV3), AAV serotype 4, (AAV4), AAV serotype 5, (AAV5), AAV serotype 8, (AAV6), derivatives thereof, or

combination thereof. For example, in some embodiments, the capsid comprises at least a portion of AAV serotype 2 and at least a portion of AAV serotype 6, e.g., AAV2.5.

[0039] The composition, e.g., the composition comprising the sc-rAAV, may be introduced into cells in a location of interest (e.g., in a canine). For example, in some embodiments when treating symptoms of osteoarthritis, the composition may be introduced into cells (e.g., chondrocytes, synoviocytes, e.g., type A, type B, etc.) in a joint via direct intraarticular injection. In some embodiments, the composition is administered to a joint, synovium, subsynovium, joint capsule, tendon, ligament, cartilage, or peri-articular muscle of the canine. The present invention is not limited to the aforementioned conditions (e.g., osteoarthritis), the means of administration (e.g., intraarticular injection), the location of interest (e.g., joint), or ceil type (e.g., chondrocytes, synoviocytes). For example, in some embodiments, other ceil types that may be transduced may include mesenchymal stem ceils. [0040] The sc-rAAV transduces the vector into cells and the modified IL-1 Ra peptide is expressed. In some embodiments, the !L-1 Ra peptide is expressed so as to provide the canine with a therapeutically effective amount of said modified IL-1 Ra peptide effective for ameliorating symptoms associated with various conditions such as osteoarthritis or rheumatoid arthritis.

[0041] In some embodiments, introduction of the composition (e.g., the sc-rAAV) is performed once. In some embodiments, introduction of the composition (e.g., the sc- rAAV) is performed twice, e.g., a first time and a second time subsequent to the first time. In some embodiments, introduction of the composition is performed more than two times, e.g., three times, four times, five times, etc. The introduction of the composition a second time may be performed at a time point after the time when the method is first performed, e.g., after 3 months, 4 months, 5 months, 8 months, 7 months, 8 months, 9 months, 10 months, 1 1 months, 1 year, more than one year, etc.

[0042] The composition may comprise any appropriate pharmaceutical composition. In some embodiments, the composition comprises a buffered solution. In some embodiments, the buffered solution comprises phosphate buffered saline (PBS). In some embodiments, the composition further comprises sorbitol, e.g., 5% sorbitol. In some embodiments, the composition further comprises a salt, e.g., NaCL The concentration of salt may be any appropriate concentration, e.g., 350 mM NaCI, more than 350 mM NaCI, less then 350 mM, etc.

[0043] In some embodiments, the composition (e.g., the sc-rAAV) is coadministered with a secondary therapy. In some embodiments, the secondary therapy comprises a therapeutic for OA or RA or any other appropriate therapy for treating the symptoms of the condition. Non-limiting examples of secondary therapies for OA include glucocorticoids, hyaluronan (viscosuppiementation), platelet-rich plasma, and recombinant, human IL-1 Ra (Anakinra; Kineret®). For example, in some embodiments, the sc-rAAV is co-administered with glucocorticoids or platelet-rich plasma.

[0044] The disclosures of the following U.S. Patents are incorporated in their entirety by reference herein: US2008/0187576, US2009/0104155, KR2012041 139, JP2015518816, WO2013151672, WO2008088895, US8529885, US7037492, US20070128177, US6491907, US8999948, US20150218586, US7892824,

US20130295614, JP2002538770, JP2010516252, KR2002027450, KR2Q03028Q80, US6482834, US20090105148, US20120232130, US20140234255, US5756283, US6083716, WO2002038782, WO2007039699, WO2012047093, WO2014170470, WO2015018860, WO2015044292, WO2015158749, US7452696, US6943153, US6429001 , VVO2015031392, WO200409221 1 ,

EXAMPLE 1

[0045] Example 1 describes general steps for making an AAV construct for expression of canine SL-1 Ra. The present invention is not limited to the disclosure of Example 1. 1 ) Synthesize a modified canine IL-1 Ra gene using the nucleotide seuqence and/or amino acid seuqence of canine IL-1 Ra. 2) Clone the modified canine gene into an AAV vector piasmid. 3) Clone in appropriate regulatory sequences, e.g., promoter, enhancer, untranslated sequences before and after the IL-1 Ra coding sequence, a polyA sequence, and potentially a WPRE. Each may be tested and validated in vitro, e.g., in combination, and in parallel. Some may need to be synthesized.

[0046] Example 2 describes administration of a sc-rAAV of the present invention (encoding IL-1 Ra). The present invention is not limited to the disclosure of Example 2.

[0047] Fifteen dogs diagnosed with osteoarthritis in the knee are used for a clinical trial investigating administration of a sc~rAAV (encoding IL-1 Ra) of the present invention. Twelve of the fifteen dogs are administered the sc-rAAV via intraarticular injection into the knee with osteoarthritis at 1 x 10 ^!° viral genes per knee. The remaining three dogs are treated with a vehicle control. After three weeks, all dogs are evaluated for lameness. At the three-month time point, the three control animals are given a second treatment of vehicle control. Three of the 12 remaining previously treated dogs are administered a second administration of the same sc-rAAV (via intraarticular injection into the knee with osteoarthritis at 1 x 10¹⁰ viral genes per knee); three are administered a second administration of a sc-rAAV (encoding IL- 1 RA) of the present invention that is different from the first sc-rAAV (via intraarticular injection into the knee with osteoarthritis at 1 x 10¹⁰ viral genes per knee); three are administered a second administration of the same sc-rAAV (via intraarticular injection into the knee with osteoarthritis at 1 x 10¹⁰ viral genes per knee) in combination with a secondary therapy (e.g., glucocorticoids and platelet-rich plasma); and the remaining three are administered a second administration of the sc-rAAV (via intraarticular injection) into the knee with osteoarthritis at 1 x 10¹⁰ viral genes per knee) in combination with an immunosuppressant. The dogs are evaluated for lameness at 3, 6, and 9 weeks post-administration (of the second administrations).

[0048] Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fail within the scope of the appended claims. Each reference cited in the present application is incorporated herein by reference in its entirety.

[0049] Although there has been shown and described embodiments of the present invention, it will be readily apparent to those skilled in the art that modifications may be made thereto which do not exceed the scope of the appended claims. Reference numbers recited in the claims are exemplary and for ease of review by the patent office only, and are not limiting in any way. In some embodiments, the figures presented in this patent application are drawn to scale, including the angles, ratios of dimensions, etc. In some embodiments, the figures are representative only and the claims are not limited by the dimensions of the figures. In some embodiments, descriptions of the inventions described herein using the phrase "comprising" includes embodiments that could be described as "consisting of, and as such the written description requirement for claiming one or more embodiments of the present invention using the phrase "consisting of is met.

[0050] Any reference numbers recited in the below claims are solely for ease of examination of this patent application, and are exemplary, and are not intended in any way to limit the scope of the claims to the particular features having the corresponding reference numbers in the drawings.

Claims

WHAT IS CLAIMED IS:

1. A method of providing a canine in need thereof a therapeutically effective

amount of interleukin-1 receptor agonist (!L-1 Ra) peptide, said method comprising: introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a promoter, the modified !L-1 Ra gene is at least 95% identical SEQ ID NO: 2;

wherein the sc-rAAV transduces the vector into ceils in the location of interest, wherein the modified IL-1 Ra gene is expressed so as to provide the canine with the therapeutically effective amount of said !L-1 Ra peptide,

2. The method of claim 1 , wherein said canine is diagnosed with or is at risk for developing osteoarthritis or rheumatoid arthritis,

3. The method of claim 1 , wherein the location of interest is a joint, synovium, subsynovium, joint capsule, tendon, ligament, cartilage, or peri-articular muscle of the canine,

4. The method of claim 1 , wherein the composition is introduced into the location of interest via direct intraarticular injection

5. The method of claim 1 , wherein the ceils are chondrocytes, synoviocytes, or a combination thereof,

8. The method of claim 1 , wherein the method is performed a second time at a time point after a time when the method is performed first.

7. The method of claim 1 , wherein the time point is at least 3 months,

8. The method of claim 1 , wherein the method further comprises co-introducing a secondary therapy to the location of interest in combination with the composition.

9. The method of claim 8, wherein the secondary therapy comprises a

glucocorticoid, hyaluronan, platelet-rich plasma, recombinant, canine !L-1 Ra, or a combination thereof.

10. The method of claim 1 , wherein the promoter comprises a CMV promoter,

1 1.The method of claim 1 , wherein the engineered capsid comprises at least a portion of serotype AAV2 and at least a portion of serotype AAV8.

12. The method of claim 1 , wherein the engineered capsid comprises at least a portion of serotype AAV1 , AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV1 1 , or a combination thereof.

13. The method of claim 1 , wherein the vector further comprises SV40 and bovine growth hormone (bGH) polyadenylation sequences.

14. The method of claim 13, wherein the vector further comprises SV40 splice donor (SD) and splice acceptor (SA) sites.

15. The method of claim 1 , wherein the vector comprises sc-rAAV2.5Hu-iL.-1 Ra.

16. A method of ameliorating symptoms of osteoarthritis or rheumatoid arthritis in a canine, said method comprising introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc~rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a promoter, the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2;

wherein the sc-rAAV transduces the vector into ceils in the location of interest, wherein the modified !L-1 Ra gene is expressed so as to provide the canine with an amount of IL-1 Ra peptide effective for ameliorating symptoms associated with osteoarthritis or rheumatoid arthritis.

17. The method of claim 16, wherein the location of interest is a joint, synovium, subsynovium, joint capsule, tendon, ligament, cartilage, or peri-articular muscle of the canine.

18. The method of claim 16, wherein the composition is introduced into the

location of interest via direct intraarticular injection

19. The method of claim 16, wherein the cells are chondrocytes, synoviocytes, or a combination thereof.

20. The method of claim 16, wherein the method is performed a second time at a time point after a time when the method is performed first.

21.The method of claim 16, wherein the time point is at least 3 months.

22. The method of claim 16, wherein the method further comprises co-introducing a secondary therapy to the location of interest in combination with the composition.

23. The method of claim 22, wherein the secondary therapy comprises a glucocorticoid, hyaluronan, platelet-rich plasma, recombinant, canine IL-1 Ra, or a combination thereof.

24. The method of claim 16, wherein the promoter comprises a CMV promoter.

25. The method of claim 16, wherein the engineered capsid comprises at least a portion of serotype AAV2 and at least a portion of serotype AAV6.

26. The method of claim 16, wherein the engineered capsid comprises at least a portion of serotype AAV1 , AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV1 1 , or a combination thereof.

27. The method of claim 16, wherein the vector further comprises SV40 and

bovine growth hormone (bGH) polyadenyiation sequences.

28. The method of claim 27, wherein the vector further comprises SV40 splice donor (SD) and splice acceptor (SA) sites.

29. The method of claim 16, wherein the vector comprises sc-rAAV2,5Hu-IL-1 Ra,

30. A method of delivering SL-1 Ra peptide to a chondrocyte or synoviocyte, said method comprising contacting the chondrocyte or synoviocyte with a recombinant self-complementary adeno-associated virus (sc~rAAV) comprising:

a. an engineered adeno-associated virus (AAV) capsid comprising at least a portion of serotype 2 and at least a portion of serotype 6; and b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a C V promoter, the modified !L-1 Ra gene is at least 95% identical to SEQ ID NO: 2;

wherein the sc-rAAV transduces the vector into the chondrocyte or synoviocyte and the modified IL-1 Ra gene is expressed to as to provide IL- 1 Ra peptide to the chondrocyte or synoviocyte.

31. The method of claim 30, wherein the vector comprises sc~rAAV2.5Hu-IL-1 Ra.

32. The method of claim 30, wherein the vector further comprises SV40 and

bovine growth hormone (bGH) polyadenyiation sequences.

33. The method of claim 30, wherein the vector further comprises SV40 splice donor (SD) and splice acceptor (SA) sites.

34. A composition comprising a recombinant self-complementary adeno- associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered capsid comprising at least a portion of serotype 2 and at least a portion of serotype 6; and

b, a vector packaged within the capsid, said vector comprises a nucleic acid sequence encoding a modified !L-1 Ra peptide operably linked to a CMV promoter, the nucleic acid sequence that encodes the modified IL-1 Ra peptide is at least 90% identical to SEQ ID NO: 2,

35. The composition of claim 34, wherein the vector further comprises SV40 and bovine growth hormone (bGH) polyadenyiation sequences.

36. The composition of claim 35, wherein the vector further comprises SV40

splice donor (SD) and splice acceptor (SA) sites.

37. The composition of claim 34, wherein the vector comprises sc-rAAV2.5Hu-IL- 1 Ra.

38. A recombinant self-complementary adeno-associated virus (sc-rAAV) vector comprising a modified IL-1 Ra gene operably linked to a CMV promoter, the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2.

39. The vector of claim 38 further comprising SV40 and bovine growth hormone (bGH) polyadenyiation sequences.

40. The vector of claim 39 further comprising SV40 splice donor (SD) and splice acceptor (SA) sites.

41.The vector of claim 38 comprising sc-rAAV2.5Hu-IL-1 Ra.

42. A method of repairing cartilage in a canine in need thereof, said method

comprising: introducing into a location of cartilage a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid: and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably linked to a promoter, the modified IL-1 Ra gene is at least 95% identical SEQ ID NO: 2;

wherein the sc-rAAV transduces the vector into cells in the location of cartilage, wherein the modified !L~1 Ra gene is expressed so as to provide the canine with IL-1 Ra peptide effective for repairing cartilage.

43. The method of claim 42, wherein said canine is diagnosed with or is at risk for developing osteoarthritis or rheumatoid arthritis.

44. The method of claim 42, wherein the composition is introduced into the

location of cartilage via direct intraarticular injection

45. The method of claim 42, wherein the cells are chondrocytes, synoviocytes, or a combination thereof.

46. The method of claim 42, wherein the method is performed a second time at a time point after a time when the method is performed first.

47. The method of claim 42, wherein the time point is at least 3 months.

48. The method of claim 42, wherein the method further comprises co-introducing a secondary therapy to the location of cartilage in combination with the composition.

49. The method of claim 48, wherein the secondary therapy comprises a

glucocorticoid, hyaluronan, platelet-rich plasma, recombinant, canine IL-1 Ra, or a combination thereof.

50. The method of claim 42, wherein the promoter comprises a CMV promoter.

51. The method of claim 42, wherein the engineered capsid comprises at least a portion of serotype AAV2 and at least a portion of serotype AAV8.

52. The method of claim 42, wherein the engineered capsid comprises at least a portion of serotype AAV1 , AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV1 1 , or a combination thereof.

53. The method of claim 42, wherein the vector comprises sc-rAAV2.5Hu-IL.-1 Ra.

54. A method of providing inter!eukin-1 receptor agonist (IL-1 Ra) peptide to an area of inflammation, said method comprising: introducing into a location of inflammation a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably linked to a promoter, the modified IL-1 Ra gene is at least 95% identical SEQ !D NO: 2;

wherein the sc-rAAV transduces the vector into cells in the location of inflammation, wherein the modified IL-1 Ra gene is expressed so as to provide the cells in the location of inflammation a therapeutically effective amount of IL-1 Ra peptide effective for reducing inflammation.

55. The method of claim 54, wherein the location of inflammation is a joint,

synovium, subsynovium, joint capsule, tendon, ligament, cartilage, or periarticular muscle of the canine,

56. The method of claim 54, wherein the composition is introduced into the location of inflammation via direct intraarticular injection

57. The method of claim 54, wherein the cells are chondrocytes, synoviocytes, or a combination thereof.

58. The method of claim 54, wherein the promoter comprises a CfvIV promoter.

59. The method of claim 54, wherein the engineered capsid comprises at least a portion of serotype AAV2 and at least a portion of serotype AAV6.

80. The method of claim 54, wherein the engineered capsid comprises at least a portion of serotype AAV1 , AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV1 1 , or a combination thereof.

61. The method of claim 54, wherein the vector comprises sc-rAAV2.5Hu-IL.-1 Ra.

62. A method of providing a canine in need thereof a therapeutically effective

amount of interleukin-1 receptor agonist (IL-1 Ra), said method comprising: introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably linked to a promoter, the modified IL-1 Ra gene encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ !D NO: 9; wherein the sc-rAAV transduces the vector into cells in the location of interest, wherein !L-1 Ra is expressed so as to provide the canine with the

therapeutically effective amount of said IL-1 Ra.

63. A method of ameliorating symptoms of osteoarthritis or rheumatoid arthritis in a canine, said method comprising introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably linked to a promoter, the modified IL-1 Ra gene encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9; wherein the sc-rAAV transduces the vector into cells in the location of interest, wherein !L-1 Ra expressed so as to provide the canine with an amount of IL- 1 Ra effective for ameliorating symptoms associated with osteoarthritis or rheumatoid arthritis.

64. A method of delivering IL-1 Ra peptide to a chondrocyte or synoviocyte, said method comprising contacting the chondrocyte or synoviocyte with a recombinant self-complementary adeno-associated virus (sc~rAAV) comprising:

a. an engineered adeno-associated virus (AAV) capsid comprising at least a portion of serotype 2 and at least a portion of serotype 6; and b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a CIV1V promoter, the modified !L-1 Ra encodes !L-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9; wherein the sc-rAAV transduces the vector into the chondrocyte or synoviocyte and IL-1 Ra is expressed to as to provide IL-1 Ra to the

chondrocyte or synoviocyte.

65. A composition comprising a recombinant self-complementary adeno- associated virus (sc-rAAV), wherein said sc-rAAV comprises:

b. a vector packaged within the capsid, said vector comprises a nucleic acid sequence encoding a modified IL-1 Ra peptide operabiy linked to a CMV promoter, the nucleic acid sequence encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ !D NO: 9.

66. A method of repairing cartilage in a canine in need thereof, said method

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a promoter, the modified IL-1 Ra gene encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9; wherein the sc-rAAV transduces the vector into cells in the location of cartilage, wherein IL-1 Ra is expressed so as to provide the canine with IL-1 Ra effective for repairing cartilage.

67. A method of providing interleukin-1 receptor agonist (IL-1 Ra) peptide to an area of inflammation, said method comprising: introducing into a location of inflammation a composition comprising a recombinant self-complementary adeno-associaied virus (sc-rAAV), wherein said sc-rAAV comprises:

a, an engineered AAV capsid; and

b, a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably iinked to a promoter, the modified IL-1 Ra gene encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ !D NO: 9; wherein the sc-rAAV transduces the vector into cells in the location of inflammation, wherein IL-1 Ra is expressed so as to provide the cells in the location of inflammation a therapeutically effective amount of IL-1 Ra effective for reducing inflammation.

68. A method of providing a canine in need thereof a therapeutically effective

amount of interleukin-1 receptor agonist (IL-1 Ra) peptide, said method comprising: introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

a. an engineered AAV capsid; and

b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably Iinked to a promoter, the modified IL-1 Ra gene is at least 95% identical SEQ ID NO: 2 and encodes IL-1 Ra according to SEQ !D NO: 8 or SEQ ID NO: 9;

wherein the sc-rAAV transduces the vector into cells in the location of interest, wherein the modified IL-1 Ra gene is expressed so as to provide the canine with the therapeutically effective amount of said IL-1 Ra peptide.

89. A method of ameliorating symptoms of osteoarthritis or rheumatoid arthritis in a canine, said method comprising introducing into a location of interest a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

c. an engineered AAV capsid; and

d. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably Iinked to a promoter, the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2 and encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9;

wherein the sc-rAAV transduces the vector into cells in the location of interest, wherein the modified IL-1 Ra gene is expressed so as to provide the canine with an amount of IL-1 Ra peptide effective for ameliorating symptoms associated with osteoarthritis or rheumatoid arthritis.

70. A method of delivering !L-1 Ra peptide to a chondrocyte or synoviocyte, said method comprising contacting the chondrocyte or synoviocyte with a recombinant self-complementary adeno-associated virus (sc-rAAV) comprising:

c. an engineered adeno-associated virus (AAV) capsid comprising at least a portion of serotype 2 and at least a portion of serotype 8; and d. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a CMV promoter, the modified !L-1 Ra gene is at least 95% identical to SEQ ID NO: 2 and encodes IL-1 Ra according to SEQ SD NO: 8 or SEQ ID NO: 9;

71. A composition comprising a recombinant self-complementary adeno- associated virus (sc-rAAV), wherein said sc-rAAV comprises:

c. an engineered capsid comprising at least a portion of serotype 2 and at least a portion of serotype 6; and

d. a vector packaged within the capsid, said vector comprises a nucleic acid sequence encoding a modified IL~1 Ra peptide operabiy linked to a CMV promoter, the nucleic acid sequence that encodes the modified IL-1 Ra peptide is at least 90% identical to SEQ ID NO: 2 and encodes !L-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9.

72. A recombinant self-complementary adeno-associated virus (sc-rAAV) vector comprising a modified IL-1 Ra gene operabiy linked to a CMV promoter, the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO: 2 and encodes IL-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9.

73. A method of repairing cartilage in a canine in need thereof, said method

c. an engineered AAV capsid; and

d. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operabiy linked to a promoter, the modified IL-1 Ra gene is at least 95% identical SEQ ID NO: 2 and encodes IL-1 Ra according to

SEQ ID NO: 8 or SEQ ID NO: 9;

wherein the sc~rAAV transduces the vector into cells in the location of cartilage, wherein the modified IL-1 Ra gene is expressed so as to provide the canine with IL-1 Ra peptide effective for repairing cartilage.

74. A method of providing interleukin-1 receptor agonist (IL-1 Ra) peptide to an area of inflammation, said method comprising: introducing into a location of inflammation a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:

c. an engineered AAV capsid: and

d. a vector packaged within the capsid, said vector comprising a modified !L-1 Ra gene operabiy linked to a promoter, the modified IL-1 Ra gene is at least 95% identical SEQ ID NO: 2 and encodes !L-1 Ra according to SEQ ID NO: 8 or SEQ ID NO: 9;