WO2017194634A1 - Agonist agents of cd47 inducing programmed cell death and their use in the treatments of diseases associated with defects in programmed cell death - Google Patents
Agonist agents of cd47 inducing programmed cell death and their use in the treatments of diseases associated with defects in programmed cell death Download PDFInfo
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Classifications
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/50—Cyclic peptides containing at least one abnormal peptide link
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/64—Cyclic peptides containing only normal peptide links
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- A—HUMAN NECESSITIES
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- A61K38/00—Medicinal preparations containing peptides
Definitions
- the present invention relates to cyclic peptides mimetics of the C- terminal binding domain of TSP-1.
- the present invention also relates to the use of these cyclic peptides as agonists of CD47 and their ability to trigger programmed cell death (PCD).
- PCD programmed cell death
- the present invention also relate to a pharmaceutical composition for use in the treatment of diseases associated with defects in PCD such as cancers and immunological disorders (including chronic inflammation) and comprising at least one cyclic peptide according to the invention.
- PCD Programmed Cell Death
- Cancers are diseases characterized by an imbalance between cell division and ceil death.”' In cancer cells, the PCD blockage is mainly caused either by cytogenetic abnormalities leading to over-expression of anti-cell death proteins or down-regulation of pro-cell death proteins. More than 50,000 chromosomal aberrations (gains or losses) have been reported to date.
- non-apoptotic PCD Although focus has been on caspase-dependent apoptotic death pathways, it is known that non-apoptotic PCD also form an important barrier against tumor initiation and progression/ Akin to the earlier landmark discoveries that lead to the identification of the major cancer-related proteins like p53, c-Myc and Bcl-2 as controllers of spontaneous and therapy-induced apoptosis, numerous proteins with properties of tumor suppressors and onco-proteins have been identified as key regulators of alternative death programs. The emerging data on the molecular mechanisms regulating non-apoptotic PCD will have potent therapeutic consequences/ 1
- Carcinogenesis is also associated with inflammation that is a defensive process against tissue injury (Zenaida Lopez-Dee, Kenneth Pidcock, and Linda S. Gutierrez in "Thrombospondin- 1 : Multiple Path to inflammation, Mediators of Inflammation, Volume 201 1, Article ID 296069, 10 pages doi:l 0.3 155/201 1/296069). Once this self-protective strategy is initiated, an effective resolution of the process is crucial to avoid major and unnecessary tissue damage.
- autoimmune diseases A common feature of autoimmune diseases is altered tolerance to self- antigens and generation of autoantibodies. Immune homeostasis and maintenance of immune tolerance are strongly dependent on apoptosis. A large number of evidence support the idea that defective apoptosis of immune cells leads to autoimmune disease.
- Lpr and gld mice defective for the Fas signaling pathway develop lympho adenopathy and splenomegaly and produce a large number of autoantibodies developing a disease that resembles human systemic lupus erythematosus (SLE), clearly demonstrating an essential role for the extrinsic apoptotic pathway in controlling auto-reactive T and B cells and the fact that alteration in apoptosis can strongly contribute to autoimmune diseases pathogenesis/" 1
- APS autoimmune lymphoproliferative syndrome
- TSP-1 is a matricellular protein of 450kD that was first isolated in activated platelets and described as a glycoprotein by Lawler and al in 1978.
- X1X In 1994 and 1996, Gao and coworkers have described the TSP-1 as an endogenous ligand for CD47 ⁇ , ⁇ ⁇ 7s .j belongs to a family of multi-domains calcium-binding glycoproteins composed of five different members.
- TSP-1 has several domains that bind to different cell membrane receptors or extracellular matrix, which mediates cell-cell and cell -extracellular matrix interactions. It contains an N-terminal globular domain, three disulfide-linked chains (the type I (properdin-like repeats), type II (epidermal growth factor-like), and type III (calcium binding) repeats) and a C- terminal globular domain.
- the N-terminal domain interacts with low-density lipoprotein receptor-related protein, heparin and several integrins.
- Type I repeats or thrombospondin structural homology repeats (TSRs) bind CD36, collagen type V, fibronectin, and heparan sulfate proteoglycans.
- the type III repeats are calcium-binding domains that bind to ⁇ 3 integrins through an RGD motif on TSP-1 411.
- the C- terminal domain of TSP-1 binds to CD47 (see Gao and Frazier).
- TSP-1 Binding of TSP-1 to CD47 influences several fundamental cellular functions including cell migration and adhesion, cell proliferation or apoptosis and plays a role in the regulation of angiogenesis and inflamrnation, x lil
- CD47/TSP-1 ligation The biological consequences of CD47/TSP-1 ligation are very vast and depend of cell types, association with other molecules, conformation, distribution on the cell surface, the mode of engagement and the particular situation in which these points occur.
- CD47-mediated cell death induction was observed in multiple tumor cells such as acute lymphoblastic leukemia cells (CCRF-CEM cell line), x x breast tumor cells (MCF- 7 cell line), xxxi multiple myeloma cells (KPMM2 cell line), xx acute promyelocyte leukemia cells (NB4 and the ATRA-refractory NB4-LR1 cell line), xxxm and histiocytic lymphoma cells (U937 cell line).
- CCRF-CEM cell line acute lymphoblastic leukemia cells
- MCF- 7 cell line xxxi multiple myeloma cells
- KPMM2 cell line xxxi multiple myeloma cells
- NB4 and the ATRA-refractory NB4-LR1 cell line xxxm and histiocytic lymphoma cells
- xxxiV Moreover, the studies done in to acute T-cell leukemia cells aurkat) xxxv
- CD47-mediated PCD Most information about CD47-mediated PCD comes from cancer cells, principally Jurkat and CLL cells.
- the main characteristics of CD47-mediated PCD in cancer cells are: 1) fast process; 2) caspase-independent; 3) Mitochondria membrane depolarization without the release of proapoptotic proteins (cytochrome C, AIF, Smac/Diablo, Orai/Htra2, endonuclease G (EndoG)); 4) reactive oxygen species (ROS) production; 5) Phosphatidylserine exposure; 6) Plasma membrane permeabilization; 7) Absence of DNA fragmentation nor chromatin condensation.
- ROS reactive oxygen species
- CD47 was shown to be overexpressed in multiple types of cancer. Moreover, it has multiple roles in immune system evasion, migration, adhesion, proliferation, and cell death, so CD47 can be exploited as a key target for cancer therapy, in multiple forms as illustrated in Figure 4: phagocytosis, stimulation of antitumor adaptative immune response, antibody dependent cellular cytotoxicity (ADCC), inhibition of CD47-dependent cellular functions and CD47-mediated PCD induction.
- ADCC antibody dependent cellular cytotoxicity
- WO 2013/182650 it was demonstrated that 4N1K, a soluble decapeptide that mimics the C-terminal domain of TSP-1, induces caspase-independent PCD in B-chronic lymphocytic leukemia (CLL) primary cells by ligation with CD47. It was further demonstrated that, contrary to the anti-CD47 mAb which needs to be immobilized to induce PCD, the soluble 4N1K peptide does not need to be coated on plastic to induce caspase-independent PCD. It was found that a negative control peptide 4NGG (4N1 mutated in two amino acids) is unable to induce PCD, signifying the specificity of the 4N1K PCD induction.
- CLL B-chronic lymphocytic leukemia
- this invention is related to a soluble peptide comprising the amino acids sequence: RFYVVMWK or a function- conservative variant thereof for use in the treatment of cancer.
- the present invention answers this first need by the design of cyclic mimetics of the TSP-1 C-terminal binding domain with high affinity (in the nanomolar range) and potency in triggering PCD (in the microM range). Surpringly, the Inventors have observed that these cyclic peptides are 100 to 1000 times more potent than those described in WO 2013/182650 (see Figures 6 and 7).
- the Inventors have now prepared new cyclic peptides derived from the C-terminal domain of TSP-1 including the sequence involved in the beta-sheets N°7 or in beta-sheets N°7 and 8 or in the beta-sheets N°6 and 7 (beta-sheets numbering according to the EMBO Journal (2004) 23, 1223-1233).
- these cyclic peptides are able to induce apoptosis of cancer cell lines with high efficiency.
- these cyclic peptides have high binding affinities (with d in the nanomolar range compared to affinities in the micromolar range for the best linear peptides described to date, PKT16, see part 3 of the examples) and are efficient at lower concentration to induce apoptosis ( ⁇ microM compared to 100 microM for the best linear derivatives, P T16, see part 2 of the examples).
- the present invention thus provides an isolated cyclic peptide derived from the C-terminal domain of TSP-1 or a biologically active derivative thereof; the present invention also relates to the use of said cyclic peptides as agonist of CD47 for treating diseases associated with defects in PCD such as cancer and immunological disorders.
- FIGS 1 and 2 Two pathways closely connected leading to different types of PCD showing that PCD is a complex network and highly regulated process.
- TSP-1 is a multidomain protein, each domain involved in biological function: the heparin-binding domain (HBD), the von Willebrand C domain (vWCD), three type 1 properdin repeats, three type 2 EGFlike repeats, seven type 3 calcium-binding repeats and the C-terminal CBD. Some of the domains interact with extracellular matrix components or membrane receptors (arrows). Key amino acid sequences responsible for TSP-1 ligation to integrins (RGD) and CD47 (RFYVVMWK) are indicated. 3B. This figure shows the CD47 and 4N1 interaction.
- TSP-1 C-terminal domain 2 A
- MRMS 2 A
- Two putative TSP-1 :CD47 interaction regions [(1) and (2), respectively] were proposed by molecular modeling (see Floquet et al, 2008). xlu .
- CD47 is used as a target to eliminate cancer cells.
- Therapeutic targeting of CD47 using monoclonal antibodies (mAb) can induce the elimination of cancer cells through multiple mechanisms.
- Anti-CD47 antibodies can stimulate an anti-tumor adaptive immune response leading to the phagocytic uptake of tumor cells by DCs and subsequent antigen presentation to CD4 and CD 8 T cells.
- an anti-CD47 antibody can eliminate tumor cells through antibody Fc-dependent mechanisms.
- Function blocking of CD47 may also promote tumor reduction by blocking several of its actions in tumor cells.
- CD47 stimulation can induce direct cell death induction. Modified from hl! .
- Binding curve measured by MST The measurement method is based on the directed movement of molecules along a temperature gradient, an effect termed "thermophoresis".
- Jurkat or Mec-1 cell membrane preparations are labeled using the Nanotemper NT-647 labeling kit as described elsewhere.xliv The labeled preparation is eluted with PBS and stored at 4°C. A stock solution of each peptide is prepared in DMSO (5mM) and then diluted with PBS.
- FIG. 6A to 6H MST curves observed for PKTD1, PKTD10, PKTDlO-1, P TD10-3, PKTD10-4, PKTD10-5, PKTD10-8 [see structures and sequences in Table I] cyclic peptide analogues of the C-terminal binding domain of TSP-1.
- the Kd (1 ,9 and 49 nM respectively).
- the Kd ratio between PKT16 and PKTD1 or PKTD10 for example highlights the fact that these cyclic analogues (i.e. PKTD or PKTD10) are much more efficient in CD47 ligation.
- Such affinities of the linear peptide analogues develop to date have never been reached.
- FIG. 7A to 7F show results of the effects of several cyclic peptides of the invention (PKTD1, PKTD7, PKTD10, PKTD1 1 , PKTD16, PKD10 and PKD10-FF) in comparison with a linear analog (PKT16) on the viability of tumor cells that were evaluated on 5 cell lines (MCF-7, human breast cancer cells; HCT-1 16, human colon cancer cells; BxPC3, human pancreas cancer cells; A549 and human lung cancer cells) by cytotoxic assay and by counting directly the number of cells.
- MCF-7 human breast cancer cells
- HCT-1 human colon cancer cells
- BxPC3, human pancreas cancer cells A549 and human lung cancer cells
- the linear analogue is not efficient at 100 microM on these cancer cell lines whereas the cyclic analogues designed to mimic a hairpin involving the beta strands 6 and 7, or 7 and 8, of the C-terminal binding domain of TSP-1 (such as PKTD1, PKTD7, PKTD9, PKTD10, PKTDl O-X-NMe, PKTD1 1 , P TDl l -NMe, P TD18, PKD8 and PKD 10 for example among others) are efficient in inducing cell death on these cancer cell lines.
- TSP-1 such as PKTD1, PKTD7, PKTD9, PKTD10, PKTDl O-X-NMe, PKTD1 1 , P TDl l -NMe, P TD18, PKD8 and PKD 10 for example among others
- the simple cyclisation of the beta strand number 7 (4N1 binding epitope of TSP-1 ) is not sufficient to improve the peptide efficiency since the cyclisation of the 4N1 sequence (peptide PKC1 ) led to a cyclic analogue that has no potency in inducing cell death.
- the present invention relates to an isolated cyclic peptide of general formula (I);
- - i is nothing or a peptidic sequence comprising between 6 and 10 amino acids derived from the beta-strand N°6 of TSP-1 of sequence YAGFVF (SEQ. ID. N° l);
- - Zi is nothing or an heterochiral sequence D-Pro-L-Pro (also designated p-P, p being a D- proline and P a L-proline) or any sequence of two amino acids or analogs of amino acid able to mimic said heterochiral sequence or mimic a beta turn, example of amino acids or analogs of amino acid of said sequence are nipecotic acid, isonipecotic acid, piperidine carboxylic acid, silaproline, thioproline and any other substituted derivative thereof (fluoro, methyl, bromo etc), pseudo proline, substituted proline, N-methyl amino acids, cyclopropyl amino acids (see Karoyan et al. Target in heterocyclic system, 2004 and Karoyan et al.
- - B 2 represents the peptidic sequence Xi-X2-X3-X 4 -X5-X6- 7-Xs-X - io (SEQ. ID. N°2) is derived from the beta-strand N°7 of TSP-1 (of sequence RFYVVMWK, SEQ. ID. N°3) wherein:
- Xi refers to nothing or serine or any amino acid with similar properties such as glycine or alanine or threonine;
- X 2 refers to nothing or arginine or any amino acid with similar properties such as homoarginine, lysine, ornithine, phenylalanine, naphtylalanine, N-methyl arginine or homophenylalanine or any other ring substituted analogues in ortho, meta or para position; for example for arginine, derivatives include any other side chain involving a guanido function and/or one or more than one amine function;
- X 3 refers to phenylalanine or any amino acid with similar properties including naphtyl alanine, homophenyi alanine or any other ring substituted analogues in ortho, meta or para position such as para-fluoro-phenylalanine, para- amino-phenyl alanine or para-nitro-phenylalanine; tyrosine or any amino acid with aromatic side chains;
- tyrosine or any amino acid with aromatic side chains phenylalanine or any amino acid with similar properties including naphtyl alanine, homophenylalanine or any other ring substituted analogues in ortho, meta or para position such as para-fluoro-phenylalanine, para-amino-phenylalanine or para- nitro-phenylalanine;
- Xs refers to valine or any amino acid with similar properties including leucine, isoleucine, terleucine, methionine;
- Xs refers to valine or any amino acid with similar properties including leucine, isoleucine, terleucine, methionine;
- X 7 refers to methionine or lysine or any amino acid with similar properties including valine, methionine, norleucine, leucine or isoleucine or terleucine;
- X 8 refers to tryptophan, tyrosine, phenylalanine, naphthyl-alanine, para- fluoro-phenylalanine, para-amino-phenylalanine, para-nitro-phenylalanine, D- prolino-tryptophane or D-prolino-homotryptophane;
- X9 refers to nothing or lysine or any amino acid with similar properties including arginine, homoarginine, ornithine, phenylalanine, naphtylalanine, N- methyl arginine or homophenylalanine or any other ring substituted analogues in ortho, meta or para position or histidine;
- X10 refers to nothing or glutamine or alanine or any amino acid with similar properties including asparagine;
- - Z2 is nothing or an heterochiral sequence D-Pro-L-Pro (also designated p-P) or any sequence of two amino acids or analogs of amino acid able to mimic said heterochiral sequence or mimic a beta turn, example of amino acids or analogs of amino acid of said sequence are nipecotic acid, isonipecotic acid, piperidine carboxylic acid, silaproline, thioproline and any other substituted derivative thereof (fluoro, methyl, bromo etc), pseudo proline, substituted proline, N-methyl amino acids, cyclopropyl amino acids (Karoyan et al.
- - B 3 is nothing or a peptidic sequence comprising between 6 and 10 amino acids derived from the beta-strand N°8 of TSP-1 (of sequence GLSV VVNS, SEQ. ID. N°4);
- Bj is a peptidic sequence comprising between 6 and 10 amino acids residues derived from the beta-strand N°6 of TSP-1 then B n is nothing and if B vulnerability is B 2 or B 3 (that is to say a peptidic sequence) then Bi is nothing;
- isolated cyclic peptide comprises between 8 and 26 amino acids, preferably between 14 and 22 amino acids; according to an other embodiment, isolated cyclic peptide comprises between 18 and 22 amino acids.
- the present invention thus encompasses cyclic peptides of formula Bj- Zi-B 2j Bi-B 2 , B 2 -Z 2 -B 3 , B2-B3, B2-B2 (each B 2 being identical or different), B 2 -Z 2 -B 2 (each B 2 being identical or different) and B 2 .
- B t comprises the following sequence: YAGFVFG
- Bi comprises the following sequence: -Xn-Y-A- G-F-V-F-G-X12-X13- (SEQ. ID. N°6) wherein:
- Xi 1 is nothing or aspartic acid or any amino acid with similar properties including glutamic acid;
- X 12 is nothing or tyrosine or any amino acid with aromatic side chains and
- Xi3 is nothing or serine or any amino acid with similar properties including glycine.
- B 3 comprises the following sequence: -Xj9-Xi 4 -X )5 - 2Q- 2 i-Xi0- 22-3 ⁇ 43- i7-Xi8- (SEQ. ID. N°36); preferably, B 3 comprises the following sequence -Xi 9-X
- Xi 4 is nothing or glycine or alanine or any amino acid with similar properties including serine;
- Xi 5 is isoleucine or leucine or alanine or any amino acid with similar properties including terleucine, valine, methionine;
- Xi 6 is lysine or alanine or any amino acid with similar properties including arginine, homoarginine, lysine, ornithine, phenylalanine, naphtylalanine, N-methyl arginine or homophenylalanine or any other ring substituted analogues (ortho, meta, para), histidine, or methionine or any amino acid with similar properties including valine, leucine, isoleucine, terleucine;
- i 7 is nothing or asparagine or alanine or any amino acid with similar properties including glutamine or lysine or any amino acid with similar properties including arginine, homoarginine, lysine, ornithine, phenylalanine, naphtylalanine, N-methyl arginine or homophenylalanine or any other ring substituted analogues (ortho, meta, para), histidine;
- Xi 8 is nothing, serine or glycine or any amino acid with similar properties;
- X] 9 is nothing or serine or alanine or any amino acid with similar properties
- X20 is serine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X21 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X22 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X23 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine,
- the isolated cyclic peptide of general formula (I) of the invention yet comprises at least parts of the beta-sheet N°7, of the beta-sheets N°7 and 8 or at least parts of the beta-sheets N°6 and 7 of the C-terminal domain of the TSP-1 but cannot be the entire sequence of the C-terminal domain of the TSP-1 (as described by Kosfeld D, Frazier WA (1993) Identification of a new cell adhesion motif in two homologous peptides from the COOH-terminal cell binding domain of human thrombospondin. J Biol Chem 268: 8806-8814), because this domain has not the same biologic activity as cyclic peptides of the invention.
- the present invention relates to an isolated cyclic peptide of general formula (ia):
- - Z] is nothing or an heterochiral sequence D-Pro-L-Pro (also designated p-P, p being a D- proline and P a L-proline) or any sequence of two amino acids or analogs of amino acid able to mimic said heterochiral sequence or mimic a beta turn, example of amino acids or analogs of amino acid of said sequence are nipecotic acid, isonipecotic acid, piperidine carboxylic acid, silaproline, fhioproline and any other substituted derivative thereof (fluoro, methyl, bromo etc), pseudo proline, substituted proline, N-methyl amino acids, cyclopropyl amino acids (see Karoyan et al.
- Zj is D-Pro-L-Pro
- - B 2 represents the pepiidic sequence (SEQ. ID. N°2) derived from the beta-strand N°7 of TSP-1 (of sequence RFYVVMWK, SEQ. ID. N°3) and comprises between 6 and 10 amino acids, wherein:
- Xi refers to nothing or serine or any amino acid with similar properties such as glycine or alanine or threonine;
- X 2 refers to nothing or arginine or any amino acid with similar properties such as homoarginine, lysine, ornithine, phenylalanine, naphtyl alanine, N-methyl arginine (RNMe) or homophenylalanine or any other ring substituted analogues in ortho, meta or para position; for example for arginine, derivatives include any other side chain involving a guanido function and/or one or more than one amine function;
- X 3 refers to phenylalanine or any amino acid with similar properties including naphtyl alanine, homophenylalanine or any other ring substituted analogues in ortho, meta or para position such as para-fluoro-phenylalanine, para- amino-phenylalanine or para-nitro-phenylalanine; tyrosine or any amino acid with aromatic side chains;
- X refers to tyrosine or any amino acid with aromatic side chains, phenylalanine or any amino acid with similar properties including naphtylalanine, homophenylalanine or any other ring substituted analogues in ortho, meta or para position such as para-fluoro-phenylalanine, para-amino-phenylalanine or para- nitro -ph enyl al anine;
- X5 refers to valine or any amino acid with similar properties including leucine, i so leucine, terleucine, methionine
- X 6 refers to valine or any amino acid with similar properties including leucine, isoleucine, terleucine, methionine
- X 7 refers to methionine or lysine or any amino acid with similar properties including valine, methionine, norleucine, leucine or isoleucine or terleucine;
- X 8 refers to tryptophan, tyrosine, phenylalanine, naphthyl-alanine, para- fluoro-phenylalanine, para-amino-phenylalanine, para-nitro-phenylalanine, D- prolino-tryptophane or D-prolino-homotryptophane;
- X refers to nothing or lysine or any amino acid with similar properties including arginine, homoarginine, ornithine, phenylalanine, naphtyl alanine, N- methyl arginine or homophenyl alanine or any other ring substituted analogues in ortho, meta or para position or histidine;
- X 1 0 refers to nothing or glutamine or any amino acid with similar properties including asparagine; X 10 may also refers to alanine;
- B 2 comprises at least the 6 amino acids -X3-X -X5-X6-X7-X8S more preferably, B 2 comprises at least the peptidic fragment -F-Y-V-V-M-W- (SEQ. ID. N°37);
- - Z 2 is nothing or an heterochiral sequence D-Pro-L-Pro (also designated p ⁇ P, p being a D- proline and P a L-proline) or any sequence of two amino acids or analogs of amino acid able to mimic said heterochiral sequence or mimic a beta turn, example of amino acids or analogs of amino acid of said sequence are nipecotic acid, isonipecotic acid, piperidine carboxylic acid, silaproline, thioproline and any other substituted derivative thereof (fluoro, methyl, bromo etc), pseudo proline, substituted proline, N-methyl amino acids, cyclopropyl amino acids (see Karoyan et al. Target in heterocyclic system, 2004 and Karoyan et al.
- Zj is D-Pro-L-Pro
- B 3 is a peptidic sequence comprising between 6 and 10 amino acids derived from the beta-strand N°8 of TSP-1 (of sequence GLSVKVVNS, SEQ. ID. N°4); B 3 comprises the following sequence: -Xi 9 -Xi4-Xi s-X20- 2i- ]6- 22-X23-X] 7-Xi8- (SEQ. ID. N°36); preferably, B3 comprises the following sequence: -Xi9-Xi 4 -Xi5-S-V-Xi6-V-V-Xn-Xi wherein: )4 is nothing or glycine or alanine or any amino acid with similar properties including serine;
- Xj 5 is isoleucine or leucine or alanine or any amino acid with similar properties including terleucine, valine, methionine;
- Xi6 is lysine or alanine or any amino acid with similar properties including arginine, homoarginine, homolysine, ornithine, phenylalanine, naphtyl alanine, N-methyl arginine (RNMe) or homophenylalanine or any other ring substituted analogues (ortho, meta, para), histidine, or methionine or any amino acid with similar properties including valine, leucine, isoleucine, terleucine;
- Xi 7 is nothing, asparagine or alanine or any amino acid with similar properties including glutamine or lysine or any amino acid with similar properties including arginine, homoarginine, homolysine, ornithine, phenylalanine, naphtyl alanine, N-methyl arginine (RNMe) or homophenylalanine or any other ring substituted analogues (ortho, meta, para), histidine;
- Xi s is nothing, serine or glycine or any amino acid with similar properties
- Xi is nothing, serine or alanine or any amino acid with similar properties
- X20 is serine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X21 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X22 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- X23 is valine or alanine or any amino acid with similar properties including leucine, isoleucine, terleucine;
- B 3 comprises at least the 6 amino acids -X15-S-V-X16-V-V-; more preferably, B 3 comprises at least the peptidic fragment -L-S-V-K-V-V (SEQ. ID. N°38);
- said isolated cyclic peptide comprises an even number of aminoacids (that is to say B 2 and B n have the same number of amino acids and both consist in a fragment of 6, 7, 8, 9 or 10 amino acids) and wherein said isolated cyclic peptide comprises between 8 and 26 amino acids, preferably between 12 and 22 amino acids; more preferably, isolated cyclic peptides of the invention consist in 12, 14, 16, 18, 20 or 22 amino acids.
- the present invention thus encompasses cyclic peptides of formula B 2 -B 3 , Z1-B 2 -B3, B 2 -Z 2 -B 3 , B 2 -B 2 (each B? being identical or different) and B 2 -Z 2 -B 2 (each B 2 being identical or different).
- B 2 and B 3 are arranged so that X5 of B 2 faces i 6 of B 3 and Xg of B 2 faces X15 of B 3 as illustrated below:
- both Zi and Z 2 can be nothing; if Z ⁇ consists in two amino acids then Z 2 is nothing and if Z2 consists in two amino acids then Zi is nothing.
- the peptides thereof according to the invention can be formulated into a composition in a neutral or salt form.
- Pharmaceutically acceptable salts include the acid addition salts (fomied with the free amino groups of the peptide) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, histidine, procaine and the like.
- the cyclic peptides of the invention are prepared as described in the experimental part.
- cyclic peptides of the invention are synthesized using a mixed solid/solution phase procedure leading to a linear peptide that is then cyclized.
- Cyclization include S-S bridges, thioether bridges, C-C bonds, C-N, ester bonds, carbon-heteroatom bonds, O-O, N-N, cyclization using scaffolds...
- biologically active derivatives include the functional variants of the peptide to which it refers. More particularly, in the context of the invention, the derivative designates “biologically active derivative of the cyclic peptide of general formula (I)" are variants retaining the biological activity and the specificity of the parent peptide. Thus, in the context of the invention, said “biologically active derivatives” have are agonist of CD47 and able trigger PCD and to treat diseases associated with defects in PCD such as cancer and immunological disorders.
- the ability to trigger PCD and the antiproliferative effect of one biologically active derivative of a given cyclic peptide of general formula (1) has to be of at least about 70%, preferably between 80 and 90%, more preferably between 90 and 99% and even more preferably 100% of the antiproliferative effect, in particular to inhibit cell proliferation, of said given cyclic peptide of general formula (1) as assessed in vitro by conventional proliferation techniques.
- the biologically active derivatives have preferably the same specificity as the cyclic peptides of general formula (I) toward cell proliferation as assessed in vitro by conventional cellular experiments.
- Said biologically active derivative can be either an allelic variant of the peptide, or a peptidomimetic variant of the peptide.
- allelic variant of the peptide has the same amino acid sequence as one cyclic peptide of general formula (I), except that one or more amino acids have been replaced by other amino acids or suppressed, the final peptide retaining the biological activity and specificity of the parent cyclic peptide of general formula (I).
- allelic variant has at least 50%, preferably 70%, preferably 80%, more preferably 90% and even more preferably 95% of identity as compared with the parent cyclic peptide of general formula (I).
- percentage of identity between two amino acid sequences denotes the percentage of amino acids residues that are identical between the two sequences to be compared, obtained after the best alignment (optimum alignment), this percentage being purely statistical and the differences between the two sequences being distributed randomly and along their entire length.
- Sequence comparisons between two amino acid sequences can be performed for example with the BLAST program available on the website http://www.ncbi.nlm.nih.gov/gorf/bl2.html the parameters used being those given by default (in particular for the parameters "open gap penalty”: 5 and “extension gap penalty”:2, the matrix selected being for example the "BLOSUM 62" matrix as suggested by the program, the percentage identity between the two sequences to be compared being calculated directly by the program).
- conservative substitutions include the substitution of one non-polar (hydrophobic) residue such as isoleucine, valine, leucine or methionine for another, the substitution of one polar (hydrophilic) residue for another such as between arginine and lysine, between glutamine and asparagine, between glycine and serine, the substitution of one basic residue such as lysine, arginine or histidine for another, or the substitution of one acidic residue, such as aspartic acid or glutamic acid or another.
- the biologically active derivative of the cyclic peptide of general formula (I) can also be a peptidomimetic variant, which is an organic molecule that mimics some properties of the parent peptide, including at least one or more properties of interest that preferably is its biological activity.
- Preferred peptidomimetics are obtained by structural modification of cyclic peptides according to the invention, preferably using unnatural amino acids, D amino acid instead of L amino acids, conformational restraints, isosteric replacement or other modifications.
- Still other preferred modifications include those intended to enhance resistance to enzymatic degradation, improvement in the bioavailability, and more generally in the pharmacokinetic properties, compared to the parent cyclic peptide of general formula (I).
- Examples of such peptidomimetics include, those molecules in which free amino groups have been derivatized to form amine hydrochlorides, p-toluene sulfonyl groups, carbobenzoxy groups, t-butyloxycarbonyl groups, chloroacetyl groups or formyl groups.
- Free carboxyl groups may be derivatized to form salts, methyl and ethyl esters or other types of esters or hydrazides.
- Free hydroxyl groups may be derivatized to form O- acyl or O-alkyl derivatives.
- the imidazole nitrogen of histidine may be derivatized to form N-im- benzylhistidine.
- Chemical derivatives also include peptides that contain one or more naturally-occurring amino acid derivatives of the twenty standard amino acids.
- 4-hydroxyproline may be substituted for proline
- 5-hydroxylysine may be substituted for lysine
- 3-methylhistidine may be substituted for histidine
- homoserine may be substituted for serine
- ornithine may be substituted for lysine.
- conservative substitution also includes the use of non-natural amino acids aimed to control and stabilize peptides or proteins secondary structures.
- non-natural amino acids are chemically modified amino acids such as prolinoamino acids, beta-amino acids, N-methylamino acids, cyclopropylamino acids, alpha,alpha-substituted amino acids as describe here below.
- These non-natural amino acids may include also fluorinated, chlorinated, brominated- or iodinated modified amino acids.
- modifications include conjugation for example with lipids or carbohydrate.
- Preferred peptidomimetic variants of the cyclic peptide of general formula (I) retain at least the biological activity and specificity of said cyclic peptide of general formula (I).
- the present invention provides an isolated cyclic peptide of general formula (I) or a biologically active derivative thereof for its use as medicine.
- the present invention also provides an isolated cyclic peptide of general formula (I) or a biologically active derivative thereof for use as agonist agent for triggering programmed cell death (PCD), as agent that activates CD47 and as agonist agent of CD47. More specifically, an isolated cyclic peptide of general formula (I) or a biologically active derivative thereof is useful for the treatment of diseases involving interaction of TSPI and CD47, in particular for the treatment of diseases associated with defects in PCD; example of diseases involving apoptosis are described by Favoloro et al (Role of apoptosis in disease, AGING, May 2012, vol.4, N°5, pp.330-349), importance of cell death in disease is also described by RA Knight and G Melino (Cell death in disease: from 2010 onwards, Cell Death and Disease (2011) 2, e202; doi:10.1038/cddis.2011.89); carcinogenesis is also associated with inflammation that is a defensive process against tissue injury, example of TSP-1 role in the
- Thrombospondin-1 Multiple Path to inflammation, Mediators of Inflammation, Volume 201 1 , Article ID 296069, 10 pages doi:10.1155/201 1/296069).
- an effective resolution of the process is crucial to avoid major and unnecessary tissue damage. If the underlying event inducing inflammation is not addressed and homeostasis is not restored, the inflammation process become chronic and lead to angiogenesis, carcinogenesis and diseases associated with immunological disorders (see Favoloro et al.) including chronic inflammation (See Lopez- Dee et al.), such as for example, multiple sclerosis, Crohn disease, psoriasis, ulcerative colitis, arthritis and asthma.
- isolated cyclic peptide of general formula (I) or a biologically active derivative thereof may be useful in the treatment of a cancer selected form the group consisting of adrenal cortical cancer, anal cancer, bile duct cancer, multiple myeloma, bladder cancer, bone cancer, brain and central nervous system cancer, breast cancer, Castleman disease, cervical cancer, colorectal cancer, endometrial cancer, esophagus cancer, gallbladder cancer, gastrointestinal carcinoid tumors, Hodgkin's disease, non-Hodgkin's lymphoma, Kaposi's sarcoma, kidney cancer, laryngeal and hypopharyngeal cancer, leukemia, liver cancer, lung cancer, mesothelioma, plasmacytoma, nasal cavity and paranasal sinus cancer, nasopharyngeal cancer, neuroblastoma, oral cavity and oropharyngeal cancer, ovarian cancer, pancreatic cancer, penile cancer, pituit
- isolated cyclic peptide of general formula (I) or a biologically active derivative thereof may be useful in the treatment of diseases associated with chronic inflammation, including immunological diseases, selected from the group consisting of multiple sclerosis, Crohn disease, psoriasis, ulcerative colitis, arthritis and asthma.
- the present invention relates to a method of therapeutically treating cancer and diseases associated with chronic inflammation by administering to a subject in need thereof an effective amount of a pharmaceutical composition comprising at least an isolated cyclic peptide of general formula (I) or a biologically active derivative thereof.
- composition comprising a cyclic peptide and/or a biologically active derivative thereof of the invention
- the present invention also relates to pharmaceutical composition
- a pharmaceutical composition comprising an isolated cyclic peptide of general formula (1) or a biologically active derivative thereof and a pharmaceutically acceptable carrier.
- the isolated cyclic peptide of general formula (I) to be incorporated in the pharmaceutical composition of the invention is selected in group consisting of PKTD1 (SEQ. ID. N°9), PKTD3 (SEQ. ID. N°10), PKTD4 (SEQ. ID. N°l l), PKTD5 (SEQ. ID. N°12), PKTD6 (SEQ. ID. N°13), PKTD7 (SEQ. ID. N°14), PKTD8 (SEQ. ID. N°15), P TD9 (SEQ. ID.
- PKTD10 SEQ. ID. N°17
- P TDlO-1 SEQ. ID. N°I 8
- P TD10-2 SEQ. ID. N°19
- P TD10-3 SEQ. ID. N°20
- PKTD10-4 SEQ. ID. N°21
- PKTD10-5 SEQ. ID. N°22
- PKTD 10-6 SEQ. ID. N°23
- PKTD10-7 SEQ. ID. N°24
- P TD10-8 SEQ. ID. N°25
- PKTD10-9 SEQ. ID. N°26
- PKTDl l SEQ. ID. N°28
- PKTD12 SEQ. ID. ID.
- PKTD14 SEQ. ID. N°31
- PKTD15 SEQ. ID. N°32
- PKTD16 SEQ. ID. N°33
- PKTD17 SEQ. ID. N°34
- PKTD18 SEQ. ID. N°35
- PKPHI2 SEQ. ID. N°39
- PKPH12P SEQ. ID. N°40
- PKD8 SEQ. ID. N°41
- PKD8FF SEQ. ID. N°42
- PKD9 SEQ. ID. N°43
- PKD10 SEQ. ID. N°44
- PKD10FF SEQ. ID. N°45
- PKTDi4 SEQ. ID.
- PKD1 1 SEQ. ID. N°47
- PKDl lRNMe SEQ. ID. N°48
- PKD12 SEQ. ID. N°49
- PKD12RNMe SEQ. ID. N°50
- PKTDi3 SEQ. ID. N°51
- PKTDiS SEQ. ID. N°52
- PKTDi2 SEQ. ID. N°53
- PKTD 10- RNMe SEQ. ID. N°54
- PKTD 10-X-RNMe SEQ. ID. N°55
- PKTD10-3-X-RNMe SEQ. ID. N°56
- PKTDil SEQ. ID. ID.
- PKTD HQ SEQ. ID. N°58
- PKTD1 1 S SEQ. ID. N°59
- PKTD1 1-RNMe SEQ. ID. N°60
- PKTD1 1-X-RNMe SEQ. ID. N°61
- suitable pharmaceutically acceptable carriers include, but are not limited to: water, salt solutions (e.g., NaCI), alcohols, gum arabic, vegetable oils, benzyl alcohols, polyethylene glycols, gelatin, carbohydrates such as lactose, amylose or starch, magnesium stearate, talc, silicic acid, viscous paraffin, perfume oil, fatty acid esters, hydroxymethylcellulose, and polyvinyl pyrolidone, lipids such as but not limited to phospholipids, sphinglipids, glycerol-fatty acid esters...
- the pharmaceutical composition of the invention can be sterilized and if desired, mixed with auxiliary agents, e. g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, coloring, flavoring and/or aromatic substances and the like which do not deleteriously react with the active compounds.
- auxiliary agents e. g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, coloring, flavoring and/or aromatic substances and the like which do not deleteriously react with the active compounds.
- auxiliary agents e. g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, coloring, flavoring and/or aromatic substances and the like which do not deleteriously react with the active compounds.
- the pharmaceutical composition of the i nvention can be a liquid solution, suspension, emulsion, tablet including sterile lyophilized formulation, pill, capsule, sustained release formulation, or powder.
- Oral formulation can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, polyvinyl pyrolidone, sodium saccharine, cellulose, magnesium carbonate, etc.
- compositions for intravenous administration are solutions in sterile isotonic aqueous buffer or a sterile lyophilized formulation to be reconstituted prior injection, such injection can be intravenous, intramuscular, subcutaneous, intrathecal, such pharmaceutical composition can also be inhaled through nasal and/or pulmonary delivery.
- the pharmaceutical composition of the invention is a liquid composition that is dedicated to be administered by injection, and for example, by intratumoral injection. Said intratumoral injection can be obtained for example by using stereotactic neurosurgery.
- This administration can be performed prior to or after a surgical operation intended to remove the tumor.
- the composition enables to inhibit the growth of the tumor and avoid dissemination of the tumor cells and the occurrence of dramatic symptoms on the subject; in the second case, the composition can be used to destroy all the tumor cells that have not be removed during the surgical operation.
- the effective dose of an isolated cyclic peptide of general formula (I) varies in function of numerous parameters such as, for example, the chosen administration method, the weight, age, sex, and the sensitivity of the individual to be treated. Consequently, the optimal dose must be determined individually, in function of the relevant parameters, by a medical specialist. In order to predict the expected active doses in human from the first animal studies presented hereunder, one can also use the fi3 ⁇ 4 and C T values as described by Rocchetti et al (2007).
- Solid-phase peptide syntheses were perfomed in polypropylene Torviq syringes fitted with a polyethylene porous disc at the bottom and closed with an appropriate piston. Solvent and soluble reagents were removed through back and forth movements. Removal of the Fmoc group was carried out with pi eridine/DMF (20%, v/v) (l x l min, 1 x 10 min). Washings between deprotection, coupling, and final deprotection steps were carried out with NMP (3 ⁇ 1 min), IPA (3 ⁇ 1 min) and NMP (3 1 min). Peptide synthesis transformations and washes were perfomed at 20 °C.
- Method A analytical HPLC was conducted on a X-Select CSH CI 8 XP column (2.5 ⁇ 30 x 4.6 mm id) eluting with 0.1 % formic acid in water (solvent A) and 0.1 % formic acid in acetonitrile (solvent B), using the following elution gradient 0-3.20 minutes: 5% to 100% B, 3.20-4 minutes 100% B, at a flow rate of 1.8 ml/minute at 40°C.
- MS mass spectra
- Method B analytical HPLC was conducted on a X-Select CSH CI 8 XP column (2.5 ⁇ 30 x 4.6 mm id) eluting with 0.1 % formic acid in water (solvent A) and 0.1 % formic acid in acetonitrile (solvent B), using the following elution gradient 0-10 minutes: 40%o to 100% B, 10-1 1 minutes 100% B, at a flow rate of 1 .8 ml/minute at 40°C.
- MS mass spectra
- Method C analytical HPLC was conducted on a X-Select CSH CI 8 XP column (2.5 ⁇ 30 x 4.6 mm id) eluting with 0.1 % formic acid in water (solvent A) and 0.1% formic acid in acetonitrile (solvent B), using the following elution gradient 0-3.20 minutes: 0% to 50% B, 3.20-4 minutes 100% B, at a flow rate of 1.8 ml/minute at 40°C.
- MS mass spectra
- Method D analytical HPLC was conducted on a X-Select CSH CI 8 XP column (2.5 ⁇ 30 x 4.6 mm id) eluting with 0.1% formic acid in water (solvent A) and 0.1 % formic acid in acetonitrile (solvent B), using the following elution gradient 0-6 minutes: 0% to 50% B, 6- 7 minutes 100% B, at a flow rate of 1.8 ml/minute at 40°C.
- MS mass spectra
- Method E Analytical HPLC was conducted on a X-Select CSH CI 8 XP column (2.5 ⁇ 30 x 4.6 mm id) eluting with 0.1 % formic acid in water (solvent A) and 0.1% formic acid in acetonitrile (solvent B), using the following elution gradient 0-3 minutes: 5% to 100% B, 3-4 minutes 100% B, at a flow rate of 1.8 ml/minute at 40°C.
- MS mass spectra
- Cyclic peptides were synthesized using a mixed solid/solution phase procedure. A typical supported synthesis is reported as described earlier (see Peptidomimetic Antibiotics Target Outer-Membrane Biogenesis in Pseudomonas aeruginosa, Nityakalyani Srinivas et al. published 19 February 2010, Science 327, 1010 (2010)), 2-Chlorotrityl chloride resin was previously swelled in anhydrous CH 2 CI 2 for 2 h.
- DIPEA diisopropyethylamine
- the unreacted sites on the resin were capped by washing with a mixture of CH 2 Ci 2 /MeOH/DiPEA (7:2: 1) followed by MeOH.
- Linear peptides were cleaved from the resin by 2 times treatment with HFIP/CH 2 CI 2 cocktail (1 :4, v/v) for 15 min each.
- the reaction mixture is filtered and the resin is sequentially rinsed with CH 2 C1 2 and MeOH.
- the filtrates are pooled and the solvents were subsequently evaporated under reduced pressure.
- the crude linear peptide was precipitated 3 times using dry-ice cold Et0 2 and recovered after centrifugations (3 * 5 min, 7800 rpm) and drying (under nitrogen flow).
- the latter system is especially suitable for the cleavage of fully protected fragments to be cyclized in solution, as it eliminates the use of a carboxylic acid in the cleavage step.
- the resulting linear peptide (1 eq.) was dissolved in DMF (1 mg/mL concentration), PyBOP (2 eq.), and HOBt (2 eq.) were added to the solution.
- the pH was adjusted to 8 by adding DIPEA (1 % v/v) and the mixture was stirred until LC- MS analysis indicated the completion of the reaction (between 2 and 20 h).
- the solvent was removed under reduced pressure.
- the crude peptide was redissolved in CH 2 C1 2 or Me-THF (50 mL) and organic layer was extracted with saturated NaHC0 3 (2 ⁇ 20 mL) and brine (2 x 20 mL), dried with Na 2 S0 and then evaporated under vacuum.
- TFA H 2 0/TIS final deprotection cocktail (95/2.5/2.5, 20 mL) was smoothly added.
- the resulting mixture was stirred for 3 h and then precipitated 3 times using dry-ice cold Et0 2 (3 x 30 mL) and recovered after centrifugations (3 ⁇ 5 min, 7800 rpm) and drying (under nitrogen flow).
- cyclic peptides of the invention were evaluated on 5 cell lines (MCF-7, human breast cancer cells; HCT-116, human colon cancer cells; BxPC3, human pancreas cancer cells and A549, human lung cancer cells) by cytotoxic assay and by counting directly the number of cells.
- PKTD1, PKTD7, PKTD9, PKTD10, PKTD10-3, PKTDlO-RNMe, PKTD 10-X-RNMe, PKTD10-4, PKTDi l, PKTDl lRNMe, PKTD12, PKTD 16, PKTD18, PKD8, PKDIO and PKD10-FF were synthesized as described in the experimental part.
- Those cyclic peptides were dissolved in DMSO at the following concentrations: 0, 5, 10, 25, 50 and 100 ⁇ .
- PKC1 is a cyclic peptide that only comprise fragment of beta-strand N°7 of TSP-1 (SEQ. ID. N°8); its structure is as follows:
- Linear PKT16 peptide [(D)Lys-(N-Me)Arg-Phe-Tyr-Val-Val-Nle-Trp- Lys-(D)Lys] was used as positive control.
- Compound CTGG (also called 4NGG, a linear peptide of sequence KRFYGGMWK ) was used as negative control.
- EMEM 10% SVF for MCF-7 and Wi38
- RPMI 1640 10% SVF for HCT-1 16 and BxPC3
- F-12K 10% SVF for A549.
- 500 cells were seeded in 96-well plates, incubated at 37°C for 24 hours, and treated by the cyclic peptides at the different concentrations and controls for 2h.
- Etopiside 40 nM was used as a positive control to induce apoptosis.
- Cells were then trypsinized, washed in cold PBS, and stained with Annexin V- FrrC (BD Pharmingen) in Annexin buffer for 15 min at room temperature. Finally, they were counterstained with 50 ⁇ 3 ⁇ 4/ ⁇ propidium iodide (Sigma) and analyzed with a FACSCalibur flow cytometer. Experiment on each cell type was repeated three times. 20,000 events per sample were analyzed in each experiment.
- Procedure B Peptides were incubated for 2 hours on HCT-116 cells.
- Superkiller Trail (ALX-201-1 15-COlO) was used as a positive pro-apoptotic control.
- Cells were analyzed by cytofluoromeiry using FITC Annexin V apoptosis detection kit wit 7- AAD from BIOLEGEND (BLE640922).
- PKTD1 , PKTD7, PKTD9, PKTD10, PKTD10- 3, PKTDlO-RNMe, PKTD 10-X-RNMe, PKTD10-4, PKTD1 1 , PKTD1 l RNMe, P TD12, P TD 16, PKTD 1 8, PKDI O and PKDI O-FF show a dose-dependent viability decrease in all cell strains (from 20 to 80% of PCD induction in 2 hours from 10 to 50 ⁇ peptide concentration). This activity is significantly higher than positive control (PKT16) which is not efficient at the concentrations tested here (efficacy to induce PCD not observed at 100 ⁇ ).
- Cyclic peptide PKCl shows no efficacy in triggering PCD whatever the concentration used (from 12,5 to 50 ⁇ , same results are observed); this result demonstrates the importance of the cyclic hairpin structure involving the beta strands 6 and 7 or 7 and 8 of TSP-1 , highlighting the fact that the simple cyclisation of the 4N1 CD47- binding epitope of TSP- 1 is not sufficient to improve its potency.
- Cyclic peptide PKDI O induces a very significant decrease of cell viability at a low dose (25 ⁇ ) after 2 hours of incubation (Figure 7F).
- cyclic peptide of the invention as very promising tools for treating diseases associated with defects in PCD such as tumors and immunological diseases (including diseases associated with chronic inflammation), either in animals or in human beings. Besides its therapeutic efficiency in reducing tumor size, those cyclic peptides do not show strong toxicity usually associated with cytotoxic drugs. It is therefore embodied as being the future therapeutic agent for treating patients suffering from tumors.
- Binding affinity measurements The binding affinities of peptides, here P T16, P TD1 , PKTD10, PKTDlO-1, PKTD10-3, PKTD10-5, PKDT10-7 and PKTD10- 8 for a membrane preparation from Jurkat and/or MEC-1 cells were measured by Microscale Thermophoresis xlv on a Monolith NT1 15-pico system (Nanotemper Technologies, Kunststoff, Germany).
- MEC-1 or Jurkat membrane preparation is labeled using the Nanotemper NT-647 labeling kit as described elsewhere. !vi
- the labeled preparation is eluted with PBS and stored at 4°C.
- a stock solution of each peptide is prepared in DMSO (5mM) and then diluted with PBS. For the peptides evaluated by MST, we have kept the concentration of the NT.
- FIG. 6A to 6H MST curve observed for PKTD1, PKTD10, PKTDlO-1, PKTD10-3, PKTD10-4, PKTD10-5, PKTD10-7, P TD 0-8 [see table II] all cyclic peptide analogues of the C-terminal binding domain of TSP-1.
- the Kd (from 0, 1 to 505 nM).
- the Kd ratio highlights the fact that these cyclic analogues are all much more efficient in CD47 ligation than the linear analogues designed from the beta-strand 7.
- Kd of these cyclic peptides is comprised between 2,8 and 50 nM; this value is highly variable depending the tumor ceils and their preparation.
- proteases such as Trypsin, Chymotrypsin and Proteinase K
- Human Serum See Karoyan et al. J. Med. Chem. 2016.
- peptides such as PKD8, PKD9 or PKD10 appeared to be fully stable toward proteases whereas PKC1 is degraded by Trypsin in less than 2 hours like the linear analogue of PKD10, i.e L-PKD10: cyclisation is not sufficient to improve the metabolic stability (PKCl) neither to improve the pharmacological profil (PKCl) but the development of a stable harpin mimetic of the C- terminal binding domain of TSP-1 led to stable analogues (at least PKD8, PKD9 and PKD10) with improved pharmacological properties and ability to induce programmed Cell death (PKD8, PKD10, PKDIO-FF at least).
- Integrin- associated protein is a receptor for the C -terminal domain of thrombospondin. J Biol Chem 271, 21 -4 (1996).
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CN201780034649.7A CN109328067B (en) | 2016-05-10 | 2017-05-10 | CD47 agonists inducing apoptosis and their use for treating diseases associated with apoptosis defects |
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