WO2017184227A3 - Recombinase genome editing - Google Patents

Recombinase genome editing Download PDF

Info

Publication number
WO2017184227A3
WO2017184227A3 PCT/US2017/016184 US2017016184W WO2017184227A3 WO 2017184227 A3 WO2017184227 A3 WO 2017184227A3 US 2017016184 W US2017016184 W US 2017016184W WO 2017184227 A3 WO2017184227 A3 WO 2017184227A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
nucleic acid
recombinase
genome editing
providing
Prior art date
Application number
PCT/US2017/016184
Other languages
French (fr)
Other versions
WO2017184227A2 (en
Inventor
George M. Church
Christopher J. Gregg
Marc J. Lajoie
Xavier RIOS
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201662291499P priority Critical
Priority to US62/291,499 priority
Priority to US201662315336P priority
Priority to US62/315,336 priority
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2017184227A2 publication Critical patent/WO2017184227A2/en
Publication of WO2017184227A3 publication Critical patent/WO2017184227A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Abstract

A method of altering a target nucleic acid sequence within a cell is provided including providing the cell with a donor nucleic acid, providing the cell with a single strand annealing protein, and providing the cell with a single strand DNA binding protein, wherein one or more or both of the single strand annealing protein and the single strand DNA binding protein is foreign to the cell, and wherein the donor nucleic acid is recombined into the target nucleic acid.
PCT/US2017/016184 2016-02-04 2017-02-02 Recombinase genome editing WO2017184227A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US201662291499P true 2016-02-04 2016-02-04
US62/291,499 2016-02-04
US201662315336P true 2016-03-30 2016-03-30
US62/315,336 2016-03-30

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16/075,281 US20210332350A1 (en) 2016-02-04 2017-02-02 Recombinase Genome Editing

Publications (2)

Publication Number Publication Date
WO2017184227A2 WO2017184227A2 (en) 2017-10-26
WO2017184227A3 true WO2017184227A3 (en) 2018-02-08

Family

ID=60116930

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/016184 WO2017184227A2 (en) 2016-02-04 2017-02-02 Recombinase genome editing

Country Status (2)

Country Link
US (1) US20210332350A1 (en)
WO (1) WO2017184227A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111690587A (en) * 2019-03-13 2020-09-22 华东理工大学 Method for centrifugally screening grease yeast strains with high oil content and application thereof
WO2020237066A2 (en) * 2019-05-23 2020-11-26 President And Fellows Of Harvard College Gene editing in diverse bacteria
AU2020301236A1 (en) * 2019-06-25 2021-11-25 Inari Agriculture Technology, Inc. Improved homology dependent repair genome editing
WO2021233975A1 (en) 2020-05-20 2021-11-25 Commissariat à l'Energie Atomique et aux Energies Alternatives In-cell continuous target-gene evolution, screening and selection
CN112194514A (en) * 2020-10-12 2021-01-08 齐齐哈尔量子生物科技有限公司 Bio-organic fertilizer starter and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144734B2 (en) * 2000-08-14 2006-12-05 The United States Of America As Represented By The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144734B2 (en) * 2000-08-14 2006-12-05 The United States Of America As Represented By The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANTONY ET AL.: "Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair", J MOL BIO, vol. 425, no. 23, 2013, pages 4802 - 4819, XP028766573 *
DATABASE GenBank [O] 2 April 2015 (2015-04-02), "single-stranded DNA-binding protein [Escherichia coli K-12]", XP055462012, Database accession no. CQR83440.1. *
DATABASE GenBank [O] 26 November 2012 (2012-11-26), "hypothetical protein PA39016_003090055 [Pseudomonas aeruginosa 39016]", XP055462020, Database accession no. ZP 07797103.1 *
DATABASE GenBank 8 February 2016 (2016-02-08), "phage recombination protein Bet [Pseudomonas aeruginosa]", XP055462028, Database accession no. WP_003088368.1 *
MEYER ET AL.: "The single-stranded DNA-binding protein of Escherichia coli", MICROBIOL REV, vol. 54, no. 4, 1990, pages 342 - 380, XP055462010 *

Also Published As

Publication number Publication date
WO2017184227A2 (en) 2017-10-26
US20210332350A1 (en) 2021-10-28

Similar Documents

Publication Publication Date Title
WO2017184227A3 (en) Recombinase genome editing
WO2016046778A3 (en) Protease-activatable bispecific proteins
WO2017070633A3 (en) Evolved cas9 proteins for gene editing
WO2017027423A9 (en) Engineered crispr-cas9 compositions and methods of use
WO2014205192A3 (en) Targeted integration
WO2018191715A3 (en) Polypeptides with type v crispr activity and uses thereof
WO2016033315A3 (en) Synthon formation
EP3303607A4 (en) Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015160895A3 (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
EP3392337A4 (en) Genetic modification non-human organism, egg cells, fertilized eggs, and method for modifying target genes
WO2016130704A3 (en) Methods and compositions for analyzing cellular components
CA2884084C (en) Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
CA3010891A1 (en) Recombinogenic nucleic acid strands in situ
WO2015089511A3 (en) Modified nucleic acid molecules and uses thereof
WO2014172470A3 (en) Methods of mutating, modifying or modulating nucleic acid in a cell or nonhuman mammal
WO2015173590A8 (en) Novel cytochrome p450 fusion protein
WO2018094356A3 (en) Compositions and methods for target nucleic acid modification
MX2018015529A (en) Tagmentation using immobilized transposomes with linkers.
WO2018053365A8 (en) Methods of nucleic acid sample preparation for analysis of cell-free dna
EP3585901A4 (en) Nucleic acid constructs comprising gene editing multi-sites and uses thereof
WO2017017673A3 (en) Stable proteins and methods for designing same
WO2015120542A8 (en) Hybrid proteins and uses thereof
WO2014199336A3 (en) Improved ngs workflow

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17786273

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 17786273

Country of ref document: EP

Kind code of ref document: A2