WO2017184227A3 - Recombinase genome editing - Google Patents

Recombinase genome editing Download PDF

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Publication number
WO2017184227A3
WO2017184227A3 PCT/US2017/016184 US2017016184W WO2017184227A3 WO 2017184227 A3 WO2017184227 A3 WO 2017184227A3 US 2017016184 W US2017016184 W US 2017016184W WO 2017184227 A3 WO2017184227 A3 WO 2017184227A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
nucleic acid
single strand
recombinase
genome editing
Prior art date
Application number
PCT/US2017/016184
Other languages
French (fr)
Other versions
WO2017184227A2 (en
Inventor
George M. Church
Christopher J. Gregg
Marc J. Lajoie
Xavier RIOS
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201662291499P priority Critical
Priority to US62/291,499 priority
Priority to US201662315336P priority
Priority to US62/315,336 priority
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2017184227A2 publication Critical patent/WO2017184227A2/en
Publication of WO2017184227A3 publication Critical patent/WO2017184227A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Abstract

A method of altering a target nucleic acid sequence within a cell is provided including providing the cell with a donor nucleic acid, providing the cell with a single strand annealing protein, and providing the cell with a single strand DNA binding protein, wherein one or more or both of the single strand annealing protein and the single strand DNA binding protein is foreign to the cell, and wherein the donor nucleic acid is recombined into the target nucleic acid.
PCT/US2017/016184 2016-02-04 2017-02-02 Recombinase genome editing WO2017184227A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US201662291499P true 2016-02-04 2016-02-04
US62/291,499 2016-02-04
US201662315336P true 2016-03-30 2016-03-30
US62/315,336 2016-03-30

Publications (2)

Publication Number Publication Date
WO2017184227A2 WO2017184227A2 (en) 2017-10-26
WO2017184227A3 true WO2017184227A3 (en) 2018-02-08

Family

ID=60116930

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/016184 WO2017184227A2 (en) 2016-02-04 2017-02-02 Recombinase genome editing

Country Status (1)

Country Link
WO (1) WO2017184227A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144734B2 (en) * 2000-08-14 2006-12-05 The United States Of America As Represented By The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144734B2 (en) * 2000-08-14 2006-12-05 The United States Of America As Represented By The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANTONY ET AL.: "Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair", J MOL BIO, vol. 425, no. 23, 2013, pages 4802 - 4819, XP028766573 *
DATABASE GenBank [O] 2 April 2015 (2015-04-02), "single-stranded DNA-binding protein [Escherichia coli K-12]", XP055462012, Database accession no. CQR83440.1. *
DATABASE GenBank [O] 26 November 2012 (2012-11-26), "hypothetical protein PA39016_003090055 [Pseudomonas aeruginosa 39016]", XP055462020, Database accession no. ZP 07797103.1 *
DATABASE GenBank 8 February 2016 (2016-02-08), "phage recombination protein Bet [Pseudomonas aeruginosa]", XP055462028, Database accession no. WP_003088368.1 *
MEYER ET AL.: "The single-stranded DNA-binding protein of Escherichia coli", MICROBIOL REV, vol. 54, no. 4, 1990, pages 342 - 380, XP055462010 *

Also Published As

Publication number Publication date
WO2017184227A2 (en) 2017-10-26

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