WO2017139778A1 - Thienopyridine carboxamides as ubiquitin-specific protease inhibitors - Google Patents

Thienopyridine carboxamides as ubiquitin-specific protease inhibitors Download PDF

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Publication number
WO2017139778A1
WO2017139778A1 PCT/US2017/017690 US2017017690W WO2017139778A1 WO 2017139778 A1 WO2017139778 A1 WO 2017139778A1 US 2017017690 W US2017017690 W US 2017017690W WO 2017139778 A1 WO2017139778 A1 WO 2017139778A1
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Prior art keywords
alkyl
heterocycloalkyl
compound
optionally substituted
alkoxy
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PCT/US2017/017690
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French (fr)
Inventor
David Joseph Guerin
Kenneth W. Bair
Justin A. Caravella
Stephanos Ioannidis
David R. LANCIA JR.
Hongbin Li
Steven Mischke
Pui Yee Ng
David Richard
Shawn E. R. SCHILLER
Tatiana Shelekhin
Zhongguo Wang
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Forma Therapeutics, Inc.
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Priority to US16/077,407 priority Critical patent/US10913753B2/en
Priority to CN201780021667.1A priority patent/CN109071560B/en
Priority to EP17708031.4A priority patent/EP3414252B1/en
Priority to CA3014192A priority patent/CA3014192A1/en
Publication of WO2017139778A1 publication Critical patent/WO2017139778A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders

Definitions

  • the present disclosure is directed to modulators of ubiquitin-specific protease 28 (USP28) and/or ubiquitin-specific protease 25 (USP25) useful in the treatment of diseases or disorders associated with USP28 and/or USP25 enzymes.
  • USP28 ubiquitin-specific protease 28
  • USP25 ubiquitin-specific protease 25
  • the disclosure is concerned with compounds and compositions inhibiting USP28 and/or USP25, methods of treating diseases or disorders associated with USP28 and/or USP25, and methods of synthesis of these compounds.
  • USP28 and USP25 are cysteine isopeptidases of the USP sub-family of DUBs containing three distinct domains: an N-terminal UBA-like domain; a pair of ubiquitin- interacting motifs (UIM) and a USP domain that is predicted to have the conserved fold of the USP sub-family (Nijman et ai., Cell 2005, 123, 773-786; Komander et al., Mol. Cell Bio. 2009, 10, 550-563). USP28 and USP25 exert their function through regulating the stability of a plethora of cellular proteins. USP28 has been characterized as a tumor-promoting factor and has been found to stabilize many oncoproteins. USP25 has been characterized as a tumor-promoting factor and as a regulator of cellular responses related to autoimmune disease, inflammation, and infectious diseases (such as viruses and bacteria).
  • MYC is a master regulator of the transcription of genes involved in cell growth, proliferation and apoptosis and is essential for tumor initiation and maintenance in many tumor types.
  • MYC is a master regulator of the transcription of genes involved in cell growth, proliferation and apoptosis and is essential for tumor initiation and maintenance in many tumor types.
  • MYC is the most frequently amplified oncogene in human cancer, with alterations in many tumor types including breast, lung and prostate.
  • Knockdown of the USP28 gene has been shown to lead to a decrease of M YC protein and an associated inhibition of growth in a panel of human cancer cell lines in vitro. (Popov, Nat. Cell Biol., 765-774).
  • LSDl ly sine- specific demethylase 1 protein.
  • LSDl is a histone demethylase that complexes with many partner proteins to control cellular pluripotency and differentiation.
  • NOTCH 1 is a potent T-cell oncogene, with >50% of T-cell acute lymphoblastic leukemia (T- ALL) cases carrying activating mutations in NOTCH ! .
  • T- ALL T-cell acute lymphoblastic leukemia
  • Increased NOTCH! protein levels have also been associated with disease progression in colon cancer.
  • NOTCH 1 rearrangements lead to constitutive pathway activation and drive tumorigenesis in many cancer types, including triple- negative breast cancer. (Stoeck et al., Cancer Discov. 2014, 4, 1 154-1167).
  • FBW7 recognizes USP28 substrates in a phosphorylation-dependent manner and targets them for ubiquitination ultimately leading to their proteasomal degradation.
  • the antagonizing roles of USP28 and FBW7 on their shared oncoprotein substrates indicate the intricate nature of protein stability control and may provide additional therapeutic opportunities for cancer treatment.
  • USP25 has also been shown to be a negative regulator of innate immune responses activated by pathogens such as viruses and bacteria. USP25 has been shown to regulate the type I interferon (IFN) signaling pathway via its deubiquitinase activity on RIG-I, TRAP2, and TRAF6 and Toll-like receptor 4 (TLR-4) signaling via its deubiquitinase activity on TRAF3 (Zhong et al., PLOS One 2013, 8, e80976; Zhong et al. Sci. Signal. 2013, 6, ra35).
  • IFN type I interferon
  • TRAF-associated family of adapter proteins have wide specificities for other cell surface receptor complexes (e.g., IL-17 receptor, TNF receptor, T cell receptor, ⁇ receptor, the Toll like receptor family, etc.), USP25 is likely to be a pleiotropic regulator of immune and inflammatory signaling in multiple disease contexts, including cancers (Walsh et al., Immunol. Rev. 2015, I, 72-92; Cui et al., Hum. Vaccin. Immunother.2014, 10, 3270-3285; Bradley and Pober, Oncogene 2001, 20, 6482-6491).
  • IL-17 receptor e.g., TNF receptor, T cell receptor, ⁇ receptor, the Toll like receptor family, etc.
  • USP25 is likely to be a pleiotropic regulator of immune and inflammatory signaling in multiple disease contexts, including cancers (Walsh et al., Immunol. Rev. 2015, I, 72-92; Cui et al., Hum. Vaccin.
  • USP25 has been reported to promote tumor invasion and metastasis (Li et al., Mol. Cell 2014, 13, 166-170). Decreased expression of USP25 in lung cancer cell lines has been shown to decrease invasion and motility in in vitro experiments and to decrease tumor metastasis in in vivo experiments in mice. Studies have shown USP25 activity abrogated via expression of miRNA-200c which down-regulates USP25 expression.
  • USP25 and miRNA-200c expression levels in tissue from lung cancer patients revealed expression profiles consistent with USP25-driven tumorigenesis (i.e., elevated levels of USP25 and decreased levels of miRNA-200c in tumor tissue versus healthy tissue increased metastatic lesions, and poor clinical prognosis in patients with elevated tumor levels of USP25).
  • USP28 and IJSP25 play important roles in promoting tumorigenesis in ceils and modulating immune responses. Its major role being in the deubiquitination and stabilization of diverse oncoproteins and epigenetic drivers and immunomodulatory proteins among other cellular factors, which are necessary for immune responses and tumor initiation and growth in humans. Inhibition of USP28 and/or USP25 with small molecule inhibitors therefore has the potential to be a treatment for cancers, autoimmune diseases, inflammatory diseases, infectious diseases, and other disorders. For this reason, there remains a considerable need for novel and potent small molecule inhibitors of USP28 and/or USP25. Summary of the Disclosir
  • a first aspect of the disclosure relates to compounds of Formula (I):
  • X is N or CR -
  • Ri is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkvl, (Ci-Ce) haloalkoxy,
  • R2 is H, (Ci-C6) alkyl, (Cj -Ce) alkoxy, (Ci-Ce) haloalkvl, (Ci-Ce) haloalkoxy,
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-C 6 ) haloaikyi, (Ci-Ce) haloalkoxy,
  • Ri, R2, or R 3 is not H; or Ri and R2 together form a (( VC ' H) cycloaikyi optionally substituted with one or more
  • Rl5, or R 2 and R3 together form a (C-i-Cs) cycloaikyi optionally substituted with one or more
  • R4 is H, (Ci-Ce) alkyl, or (Ci-Ce) haloaikyi;
  • R5 is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloaikyi,
  • Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
  • each R'/ is independently at each occurrence H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • R9, Rio, Rn, R12, R13, and Ri 4 is independently H, (Ci-Ce) alkyl, or
  • each Ris is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • each Rie is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C 3 -Cs) cycloalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloaikyi; or two Ri& together when attached to the same carbon can form -C (0) when R& is
  • each Ri7 is independently at each occurrence (Ci-C 6 ) alkyl, (Ci-C 6 ) alkoxy,
  • each Rig is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • each Ri9 and R20 is independently H, (Ci-Ce) alkyl, (C3-C8) cycloalkyl, -CH 2 C(0)NH2, -S(0) 2 (Ci-C6) alkyl, -S(0) 2 (C6-Cio) aryl or -C(0)(Ci-Ce) alkyl,
  • each R21 and R22 is independently H or (Ci-Ce) alkyl; each R2.3 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP28.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP25.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP28 and USP25.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a phannaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure is directed to a method of inhibiting USP28.
  • the method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure is directed to a method of inhibiting USP25.
  • the method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure is directed to a method of inhibiting USP28 and USP25.
  • the method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating cancer. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating inflammation.
  • the method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating an autoimmune disease.
  • the method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating an infectious disease.
  • the method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
  • Another aspect of the disclosure relates to a method of treating a viral infection.
  • the method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the disclosure relates to a method of treating a bacterial infection.
  • the method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • compositions compri sing a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof and a pharmaceutically acceptable carrier.
  • the pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.
  • Another aspect of the present disclosure relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in the manufacture of a medicament for treating a disease associated with inhibiting USP28.
  • Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP28.
  • Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP25.
  • Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP28 and USP25.
  • the present disclosure further provides methods of treating a disease or disorder associated with modulation of USP28 and/or USP25 including, cancer, inflammation, an autoimmune disease, a viral infection, and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • a disease or disorder associated with modulation of USP28 and/or USP25 including, cancer, inflammation, an autoimmune disease, a viral infection, and a bacterial infection
  • the present disclosure provides inhibitors of USP28 and/or USP25 that are therapeutic agents in the treatment of diseases, such as cancer, inflammation, autoimmune diseases, viral infections, and bacterial infections.
  • diseases such as cancer, inflammation, autoimmune diseases, viral infections, and bacterial infections.
  • present disclosure provides the medical community with a novel pharmacological strategy for the treatment of diseases and disorders associated with USP28 and/or USP25 enzymes.
  • the present disclosure relates to compounds and compositions that are capable of inhibiting the activity USP28 and/or USP25.
  • the disclosure features methods of treating, preventing or ameliorating a disease or disorder in which USP28 and/or USP25 plays a role by administering to a patient in need thereof a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • the methods of the present disclosure can be used in the treatment of a variety of USP28 and/or USP25 dependent diseases and disorders by inhibiting the activity of USP28 and/or USP25 enzymes.
  • Inhibition of USP28 and/or USP25 provides a novel approach to the treatment, prevention, or amelioration of diseases including, but not limited to, cancer,
  • an alkyl group can (but is not required to) be bonded other substituents (e.g., heteroatoms).
  • substituents e.g., heteroatoms.
  • an alkyl group that is optionally substituted can be a fully saturated alkyl chain (e.g., a pure hydrocarbon).
  • the same optionally substituted alkyl group can have substituents different from hydrogen. For instance, it can, at any point along the chain be bounded to a halogen atom, a hydroxyl group, or any other substituent described herein.
  • substituents used in the optional substitution of the described groups include, without limitation, halogen, oxo, -OH, -CN, -COOH, -CH2CN, -O-(Ci-Ce) alkyl, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci- Ce) haloalkyl, (Ci-Ce) haioalkoxy, -0-(C 2 -C 6 ) alkenyl, -0-(C 2 -C 6 ) aikynyl, (C 2 -C 6 ) alkenyl, (C2- Ce) ai kynyl, -OFF -OP(0)(OH) 2 , -OC(0)(Ci-C6) al kyl , -C(0)(Ci-Ce) alkyl
  • substituted means that the specified group or moiety bears one or more suitable substituents wherein the substituents may connect to the specified group or moiety at one or more positions.
  • an aryl substituted with a cycloalkyi may indicate that the cycloalkyi connects to one atom of the aryl with a bond or by fusing with the aryl and sharing two or more common atoms.
  • aryl refers to cyclic, aromatic hydrocarbon groups that have 1 to 3 aromatic rings, including monocyclic or bicyclic groups such as phenyl, biphenyl or naphthyl. Where containing two aromatic rings (bicyclic, etc.), the aromatic rings of the aryl group may be joined at a single point (e.g., biphenyl), or fused (e.g., naphthyl). The aryl group may be optionally substituted by one or more substituents, e.g., 1 to 5 substituents, at any point of attachment.
  • substituents include, but are not limited to, -H, -halogen, -O-(Ci-Ce) alkyl, (Ci-Ce) alkyl, -0-(C2-Ce) alkenyl, -0-(C2-Ce) aikynyl, (C2-C6) alkenyl, (C 2 -C 6 ) alkynyl, -OH, -OP(0)(QH) 2 , -OC(0)(C i-C6) alkyl, -C(0)(Ci-C6) alkyl, -OC(0)0(Ci-Ce) alkyl, -NH2, NH((Ci-C 6 ) alkyl), N((Ci-C 6 ) alkyl) 2 , -S(0) 2 -(Ci-C 6 ) alkyl, -S(0)NH(Ci-Cfi) alkyl, and -S(0)N((C 1 -Ce) alkyl)
  • aryl groups herein defined may have an unsaturated or partially saturated ring fused with a fully saturated ring.
  • Exemplary ring systems of these aryl groups include, but are not limited to, phenyl, biphenyi, naphthyl, anthracenyl, phenalenyl, phenanthrenyl, indanyl, indenyl, tetrahydronaphthalenyl, tetrahydrobenzoannulenyi, and the like.
  • heteroaryl means a monovalent monocyclic aromatic radical of 5 to 24 ring atoms or a polycyclic aromatic radical, containing one or more ring heteroatoms selected from N, O, and S, the remaining ring atoms being C.
  • Heteroaryl as herein defined also means a bicyclic heteroaromatic group wherein the heteroatom is selected from N, O, and S.
  • the aromatic radical is optionally substituted independently with one or more substituents described herein.
  • Examples include, but are not limited to, fury] , thienyl , pyrrolyl, pyridyl, pyrazolyl, pyrimidinyl, imidazolyl, isoxazolyl, oxazolyi, oxadiazolyl, pyrazinyl, indolyl, thiophen-2-yl, quinolyl, benzopyranyl, isothiazolyl, thiazolyl, thiadiazole, indazole, benzimidazolyl, thieno[3,2-b]thiophene, triazolyl, triazinyl, imidazo[l ,2-b]pyrazolyl, furo[2,3- cjpyridinyl, imidazo[l,2-a]pyridinyl, indazolyl, pyrrolo[2,3-c]pyridinyl, pyrrolo[3,2-c]pyridinyl, pyrazolo[3,
  • heteroaryl groups herein defined may have an unsaturated or partially saturated ring fused with a fully saturated ring.
  • exemplary ring systems of these heteroaryl groups include indoliny] , indolinonyl, dihydrobenzothiophenyl, dihydrobenzofuran, chromanyl, thiochromanyl, tetrahydroquinolinyi, dihydrobenzothiazine, 3,4- dihydro-lH ⁇ isoquinolinyl, 2,3 -dihydrobenzofuran, indolinyl, indolyl, and dihydrobenzoxanyl.
  • Halogen or "halo" refers to fluorine, chlorine, bromine, or iodine.
  • Alkyl refers to a straight or branched chain saturated hydrocarbon containing 1-12 carbon atoms.
  • Examples of a (Ci-Ce) alkyl group include, but are not limited to, methyl, ethyl, propyl, butyl, pentyi, hexyl, isopropyl, isobutyi , sec-butyl, fert-butyl, isopentyl , neopentyl, and isohexyi.
  • Alkoxy refers to a straight or branched chain saturated hydrocarbon containing 1- 12 carbon atoms containing a terminal "O" in the chain, e.g., -O(alkyl).
  • alkoxy groups include without limitation, methoxy, ethoxy, propoxy, butoxy, t-butoxy, or pentoxy groups.
  • alkyiene or " alkyl enyl” refers to a divalent alkyl radical . Any of the above mentioned monovalent alkyl groups may be an aikyiene by abstraction of a second hydrogen atom from the alkyl .
  • alkvlene may also be a Co-Ce alkvlene.
  • An alkvlene may further be a Co-C 4 aikyiene.
  • Cycloalkyl or “carbocyclyl” means monocyclic or polycyclic saturated carbon rings containing 3-18 carbon atoms.
  • Examples of cycloalkyl groups include, without limitations, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptanyi, cyclooctanyl, norboranyi, norborenyl, bicyclo[2.2.2]octanyl, or bicyclo[2,2.2]octenyl and derivatives thereof.
  • a C: ⁇ -Cg cycloalkyl is a cycloalkyl group containing between 3 and 8 carbon atoms.
  • a cycloalkyl group can be fused (e.g., decalin) or bridged (e.g., norbomane).
  • "Heterocyclyl” or “heterocycloalkyl” monocyclic or polycyclic rings containing carbon and heteroatoms taken from oxygen, nitrogen, or sulfur and wherein there is not delocalized ⁇ electrons (aromaticity) shared among the ring carbon or heteroatoms.
  • the heterocycloalkyl ring structure may be substituted by one or more substituents. The substituents can themselves be optionally substituted.
  • heterocyclyl rings include, but are not limited to, oxetanyl, azetadinyl, tetrahydrofuraiiyl, tetrahydropyraiiyl, pyrroiidiiiyl, oxazolinyl, oxazolidinyi, thiazolinyi, thiazolidinyl, pyranyl, thiopyranyl, tetrahydropyranyl, dioxalinyi, piperidinyl, morpholinyl, thiomorpholinyl, thiomorpholinyl S-oxide, thiomorpholinyl S-dioxide, piperazinyl, azepinyl, oxepinyl, diazepinyl, tropanyl, oxazolidinonyl, and homotropanyl.
  • hydroxyalkyl means an alkyl group as defined above, where the aikyi group is substituted with one or more OH groups.
  • hydroxyalkyl groups include HO- CH 2 ⁇ , HO-CH2-CH2- and Cft-CHfQH)-.
  • haloalkyl refers to an alkyl group, as defined herein, which is substituted one or more halogen.
  • haloalkyl groups include, but are not limited to, trifluoromethyi, difluoromethyl, pentafiuoroethyi, trichloromethyi, etc.
  • haloalkoxy refers to an alkoxy group, as defined herein, which is substituted one or more halogen.
  • haloalkyl groups include, but are not limited to, trifluoromethoxy, difluoromethoxy, pentafluoroethoxy, trichloromethoxy, etc.
  • cyano as used herein means a substituent having a carbon atom joined to a nitrogen atom by a triple bond, e.g., C ⁇ N.
  • Spirocycloalkyl or "spirocyclyl” means carbogenic bicyclic ring systems with both rings connected through a single atom.
  • the ring can be different in size and nature, or identical in size and nature. Examples include spiropentane, spriohexane, spiroheptane, spirooctane, spirononane, or spirodecane.
  • One or both of the rings in a spirocycle can be fused to another ring carbocyclic, heterocyclic, aromatic, or heteroaromatic ring.
  • a (C 3 -C 12 ) spirocy cloalkyl is a spirocycle containing between 3 and 12 carbon atoms.
  • One or more of the carbon atoms can be substituted with a heteroatom.
  • the term "spiroheterocycloalkyl" or “spiroheterocyclyl” is understood to mean a spirocycle wherein at least one of the rings is a heterocycle (e.g., at least one of the rings is furanyl, morpholinyl, or piperadinyl).
  • solvate refers to a complex of variable stoichiometry formed by a solute and solvent. Such solvents for the purpose of the disclosure may not interfere with the biological activity of the solute. Examples of suitable solvents include, but are not limited to, water, MeOH, EtOH, and AcOH. Solvates wherein water is the solvent molecule are typically referred to as hydrates. Hydrates include compositions containing stoichiometric amounts of water, as well as compositions containing variable amounts of water.
  • the term "isomer” refers to compounds that have the same composition and molecular weight but differ in physical and/or chemical properties. The structural difference may be in constitution (geometric isomers) or in the ability to rotate the plane of polarized light (stereoisomers). With regard to stereoisomers, the compounds of Formula (I) may have one or more asymmetric carbon atom and may occur as racemates, racemic mixtures and as individual enantiomers or diastereomers.
  • compositions comprising an effective amount of a disclosed compound and a pharmaceutically acceptable carrier.
  • pharmaceutically acceptable salts include, e.g., water-soluble and water-insoluble salts, such as the acetate, amsonate (4,4-diaminostilbene-2,2-disulfonate), benzenesulfonate, benzonate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium, calcium edetate, camsylate, carbonate, chloride, citrate, clavulariate, dihydrochloride, edetate, edisylate, estolate, esylate, fumerate, fiunarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexafluorophosphate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxyna
  • a "patient” or “subject” is a mammal, e.g., a human, mouse, rat, guinea pig, dog, cat, horse, cow, pig, or non-human primate, such as a monkey, chimpanzee, baboon or rhesus.
  • an "effective amount" when used in connection with a compound is an amount effective for treating or preventing a disease in a subject as described herein.
  • carrier encompasses carriers, excipients, and diluents and means a material, composition or vehicle, such as a liquid or solid fil ler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting a pharmaceutical agent from one organ, or portion of the body, to another organ, or portion of the body of a subject.
  • treating refers to improving at least one symptom of the subject's disorder. Treating includes curing, improving, or at least partially ameliorating the disorder.
  • disorder is used in this disclosure to mean, and is used interchangeably with, the terms disease, condition, or illness, unless otherwi se indicated,
  • administer refers to either directly administering a disclosed compound or pharmaceutically acceptable salt of the disclosed compound or a composition to a subject, or administering a prodrug derivative or analog of the compound or pharmaceutically acceptable salt of the compound or composition to the subject, which can form an equivalent amount of active compound within the subject's body.
  • prodrug means a compound which is converti ble in vivo by metabolic means (e.g., by hydrolysis) to a disclosed compound.
  • cancer includes, but is not limited to, the following cancers: bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g. : squamous cell carcinoma), colorectal cancer (e.g., adenocarcinoma), esophageal cancer (e.g., squamous cell carcinoma), gastric cancer (e.g.
  • adenocarcinoma medulloblastoma, colon cancer, choriocarcinoma, squamous cell carcinoma), head and neck cancer, hematologic cancer (e.g., acute lymphocytic anemia, acute myeloid leukemia, acute lymphoblastic B cell leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt' s lymphoma, chronic lymphocytic leukemia, chronic eosinophillic leukemia/hypereosinophillic syndrome, chronic myeloid leukemia, Hodgkin' s lymphoma, mantle ceil lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchi oloalveolar adenocarcinoma, mesothelioma, mucoepidermoid carcinoma, small-cell lung cancer, non-small cell lung cancer, a
  • autoimmune di sease or "autoimmune disorder” refer to a condition that is immune-mediated due to an attack on self-tissues, such as when a subject's own antibodies react with host tissue, but can also involve an immune response to a microorganism .
  • autoimmune diseases include, but are not limited to, multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto's disease, Guillain-Barre' syndrome, Grave's disease, myasthenia gravis, Sjogren's syndrome, nodular panarteritis, autoimmune enteropathy,
  • the present disclosure relates to compounds or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, capable of inhibiting USP28 and/or USP25, which are useful for the treatment of diseases and disorders associated with modulation of a USP28 and/or USP25 enzyme.
  • the disclosure further relates to compounds, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, which are useful for inhibiting USP28 and/or USP25.
  • the cancer can be any cancer in any organ, for example, a cancer is selected from the group consisting of glioma, thyroid carcinoma, breast carcinoma, small-cell lung carcinoma, non-small-cell carcinoma, gastric carcinoma, colon carcinoma, gastrointestinal stromal carcinoma, pancreatic carcinoma, bile duct carcinoma, CNS carcinoma, ovarian carcinoma, endometrial carcinoma, prostate carcinoma, renal carcinoma, anaplastic large-cell lymphoma, leukemia, multiple myeloma, mesothelioma, and melanoma, and combinations thereof.
  • a cancer is selected from the group consisting of glioma, thyroid carcinoma, breast carcinoma, small-cell lung carcinoma, non-small-cell carcinoma, gastric carcinoma, colon carcinoma, gastrointestinal stromal carcinoma, pancreatic carcinoma, bile duct carcinoma, CNS carcinoma, ovarian carcinoma, endometrial carcinoma, prostate carcinoma, renal carcinoma, anaplastic large-cell lymphoma, leukemia, multiple myeloma, mesotheliom
  • the present invention relates to compounds or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, capable of inhibiting USP28 and/or USP25, which are useful for the treatment of diseases and disorders associated with modulation of a USP28 and/or USP25 enzyme.
  • the invention further relates to compounds, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, which are useful for inhibiting USP28 and/or USP25.
  • the compounds of Formula (I) have the structure of Formula (la):
  • X is N or CR-;
  • Ri is I f , (C !-( ' ;>) alkyl, (C1-C0) alkoxy, (Ci-Gs) haloalkyl, (Ci-Ce) haloalkoxy,
  • R2 is H, (C1-C&) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloaikyi, (Ci-Ce) haloalkoxy,
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloaikyi, (Ci-Ce) haloalkoxy,
  • Ri, R2, or R3 is not H; or Ri and R2 together form a (C i ⁇ C ⁇ ; cycloalkyl optionally substituted with one or more
  • R4 is H, (Ci-Ce) alkyl, or (Ci-Ce) haloaikyi;
  • R? is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloaikyi;
  • Rs' is H, (Ci-Ce) alkyl, halogen, or (C i ⁇ ( ' .-. ⁇ haloaikyi;
  • Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
  • each R? is independently at each occurrence H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • Re and R ⁇ together when on adjacent atoms form a (Ci-Cs) cycloalkyl ring optionally substituted with one or more Ris; or Re and R7 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Rig; Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one or more Rig; or Re and R? together when on adjacent atoms form a heteroaryi ring optionally substituted with one or more Ris; or two R? together when on adjacent atoms form a (tVCg) cycloalkyl ring; or two R? together when on adjacent atoms form a heterocycloalkyl ring; two R? together when on adjacent atoms form an aryl ring; or two R? together when on adjacent atoms form a heteroaryi ring;
  • each R9, Rio, R11, R12, R13, and R 14 is independently H, (Ci-Ce) al kyl , or
  • each Ri5 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • each Ri6 is independently at each occurrence (C i -( V.) alkyl, (d-Ce) alkoxy,
  • (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -C(0) R2iR22, - S(0 ⁇ '(C !-C,-. ⁇ alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl , or two R 16 together when attached to the same carbon can form -C (0) when R 6 is
  • each Ri7 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • each Ri9 and R20 is independently H, (Ci-Ce) alkyl, (CB-CS) cycloalkyl, -CH 2 C(0)NH 2 , •S ⁇ ()) H( : ⁇ ( ' ,. ⁇ alkyl, -S(0)2(Ce-Cio) aryl or C(0) ⁇ (V( V) alkyl; each R?j and R22 is independently H or (
  • the compounds of Formula (I) have the structure of Formula (Ic):
  • the compounds of Formula (I) have the structure of Formula (Id):
  • the compounds of Formula (I) have the structure of Formula (Ie):
  • the compounds of Formula (I) have the structure of Formula (If):
  • the compounds of Formula (I) have the structure of Formula (3 ⁇ 4):
  • the compounds of Formula (I) have the structure of Formula (Hi):
  • the compounds of Formula (I) have the stracture of Formula (li):
  • the compounds of Formula (I) have the structure of Formula (3 ⁇ 4):
  • the compounds of Formula (I) have the structure of Formula (Ik):
  • R& is -(Co-Cs) alkylene-C(0)OH, -(C0-C3) alkylene-heteroeycloafkyf,
  • each Hie. is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
  • (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hvdroxyalkyl, halogen, (C 3 -Cs) cycioalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl; or two Ri6 together when attached to the same carbon can form -C (0) when e is
  • X is CR7. In another embodiment, X is
  • Ri is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C 3 -Cs) cycloalkyl, or -NR9R10,
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloal kyl , (Ci-C 4 ) haloalkoxy, (Ci-C 4 ) hydroxyalkyl, halogen, (C3-Ce) cycloalkyl, -CN, or -NR9R10.
  • Ri is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl,
  • Ri is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl,
  • Ri is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, -CN, or -NR9R10.
  • Ri is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (Cs-Ce) cycloalkyl, or -NR9R10.
  • Ri is H or (C1-C4) alkyl .
  • Ri is (Ci-C 4 ) alkyl. [0085] In some embodiments of the Formulae above, R?. is H, (C i -( ' i) alkyl,
  • R2 is H, (C1-C3) alkyl ,
  • R2 is H, (C1-C4) alkyl
  • R2 is H, (C1-C3) alkyl, (C1-C3) haloalkyl, halogen, (tVCe) cycloalkyl, or -NR11R12.
  • R2 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
  • R2 is H or halogen.
  • Ri and R2 together form a (C4-C») cycloalkyl optionally substituted with one to three R15 , In yet another embodiment, Ri and R2 together form a
  • (Gi-Ce) cycloalkyl optionally substituted with one to three Ris.
  • R3 is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (Ci-d) haloalkoxy, (Ci-Ci) hydroxyalkyl, halogen, (CVCe) cycloalkyl, or - R13R14.
  • R3 is H, ( €- - €3) alkyl, (O-C3) alkoxy, (C1-C3) haloalkyl, (O-C3) haloalkoxy, hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR13R14.
  • R3 is H, (C1-C4) alkyl, (C1-C4) alkoxy, or (C1-C4) haloalkyl.
  • R3 i s H, (O-C3) alkyl, (C1-C3) alkoxy, or (C1-C3) haloalkyl.
  • R3 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
  • R3 is H.
  • At least one of Ri, R 2 , or R3 is not H.
  • Ri is (Ci-Cc) alkyl, (Ci-Ce) alkoxy, (C1-C0) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NR R10; and R?.
  • Ri i s II (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or - R9R10; and R 2 is (Ci-Ce) alkyl, (Ci-Cc) alkoxy, (Ci-Ce) haloalkyl, (Ci-Cc) haloalkoxy, (Ci- Ce) hydroxyalkyl, halogen, (C3-C8) cycloalkyl, or -NRnR 12 .
  • Ri is (C 1-C4) alkyl, (d-C 4 ) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10; and R2 is I f .
  • Ri is H, (Ci-Ce) alkyl, (C1-C0) alkoxy, (C1-C0) haloalkyl, (Ci-Gs) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NR9R10;
  • R2 is (Ci-Cb) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cs) cycloalkyl, or -NR11R12; and
  • R3 is H, (Ci-Ce) alkyl, (Ci-C 6 ) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) hal
  • Rj is (C1-C4) alkyl, (Ci-C 4 ) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10;
  • R2 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (iVO) haloalkyl, (C1-C3) haloalkoxy,
  • (C3-C6) cycloalkyl, or -NR RM and R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cg) cycloalkyl, or -NRBRM.
  • Ri is H, (Ci-C 4 ) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10;
  • R 2 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy,
  • R2 and R3 together form a (C 4 -Cs) cycloalkyl optionally substituted with one to three R15.
  • R2 and R3 together form a (C4-C0) cycloalkyl optionally substituted with one to three R15.
  • R4 is H, (C1-C3) alkyl, or
  • R3 ⁇ 4 is H or (C 1 -C 3 ) alkyl.
  • R 4 is H.
  • R 4 is H, methyl, ethyl, n-propyl, or iso-propyl.
  • R4 is H or methyl.
  • R5 is H, (C1-C3) alkyl, halogen, or (Ci-C 3 ) haloalkyl.
  • R5 is H, halogen, or (C1-C3) alkyl.
  • R5 is H or (Ci-C 3 ) alkyl.
  • R5 is H.
  • Rs is H, F, CI, methyl, ethyl, n-propyl, or iso-propyl.
  • Rs is H, methyl, ethyl, n-propyl, or iso-propyl.
  • Rs is H, F, or methyl.
  • R5 is H or methyl.
  • R.v is H, (C1-C3) alkyl, halogen, or (Ci ⁇ C 3 ) haloalkyl.
  • Rs' is H, halogen, or (C1-C3) alkyl.
  • R.v is H or (C1-C3) alkyl.
  • R.v is H.
  • Rs' is H, F, CI, methyl, ethyl, n-propyl, or iso-propyl.
  • Rs' is H, methyl, ethyl, n-propyl, or iso-propyl.
  • Rs' is H, F, or methyl.
  • Rs 1 is H or methyl.
  • R3 ⁇ 4 and R5 together with the carbon atoms to which they are attached form a (C 3 -Cs) cycloalkyl ring.
  • R and Rs together with the carbon atoms to which they are attached form a (C 4 -Ce) cycloalkyl ring.
  • m is 2 and R 4 and R5 together with the carbon atoms to which they are attached form a (C 4 -Ce) cycloalkyl ring.
  • Re is -(Co ⁇ C?) alkyl ene-C(0)OH
  • Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl
  • Re is -(Co-C 3 ) alkylene-C(0)OH, -(Co-C 3 ) alkylene-heterocycloalkyl, -(C0-C3) alkylene-heteroaryl or -N(Rs)-(C 0 -C 3 ) alkylene-heterocycloalkyl, wherein the heterocycloalkyl and heteroaryl are optionally substituted with one to three R 16 .
  • Re is -(C0-C3) alkylene-heterocycloalkyl, -(C0-C3) alkylene-heteroaryl or
  • e is -O- heterocycloalkyl optionally substituted with one to three R10.
  • R? is H, (Ci-C 4 ) alkyl, (Ci-C 4 ) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, halogen, -OH, -CN, (C -C 8 ) cycloalkvl, heterocycloalkyl, aryl, or heteroaryl, wherein the alkyl is optionally substituted with one or more (Ci-Gs) aikoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one to three R17.
  • R? is H, (Ci-C 4 ) alkyl, (Ci-C 4 ) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, halogen, -OH, -CN, (C -C 8 ) cycloal
  • Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one to three R 18 .
  • Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one to three R 18 .
  • Re and R? together when on adjacent atoms form a heteroaryl ring optionally substituted with one to three Rie.
  • R? together when on adjacent atoms form a heterocycloalkyl ring.
  • two R? together when on adjacent atoms form an aryl ring.
  • two R? together when on adjacent atoms form a heteroaryl ring.
  • Rs is H or (C1-C3) alkyl.
  • R.8 is H.
  • Rs is (C1-C3) alkyl.
  • Rg is H, methyl, ethyl, n-propyl, or iso-propyl.
  • Rs is H, methyl, or ethyl .
  • R is H, (C1-C3) alkyl, or
  • R9 is H, (Ci-C.3) alkyl, or -C(0)(Ci ⁇ C2) alkyl.
  • Rs is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • Rio is H, (C1-C3) alkyl, or
  • R 10 is H, (C1-C3) alkyl, or -C(0)(C 1 -C 2 ) alkyl.
  • Rio is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • R11 is H, (C1-C3) alkyl, or
  • R11 is H, (C1-C3) alkyl, or -C(0)(Ci-C2) alkyl. In yet another embodiment, R11 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • Rn is H.
  • R12 is H, (C1-C3) alkyl, or
  • R12 is H, (C1-C3) alkyl, or ⁇ ' (()) ⁇ ' !-( " ⁇ ) alkyl.
  • R 12 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • R12 is H.
  • R13 is H, (C1-C3) alkyl, or
  • R 13 is H, (C1-C3) alkyl, or -C(0)(Ci ⁇ C2) alkyl.
  • R 13 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • R13 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or -C(0)CH 2 CH 3 . In another embodiment, R 13 is H,
  • R14 is H, (C1-C3) alkyl, or
  • R14 is H, (C1-C3) alkyl, or -C(0)(Ci-C2) alkyl.
  • R 14 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or
  • RM is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH 3 , or -C(0)CH 2 CH 3 .
  • R 14 is H, j 1 [00111]
  • Ris is (C1-C3) aikyi, (C1-C3) alkoxy, (d-C 3 ) haloalkyl, (C1-C3) haloalkoxy, halogen, or -OH.
  • R15 is
  • R15 is
  • R15 is (C1-Cs) alkyl, halogen, or -OH.
  • R15 is (C1-C3) aikyi or -OH.
  • R15 is -OH.
  • R10 is (Ci-CV) alkyl, (Ci-C 4 ) alkoxy, (Ci-C 4 ) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxy alkyl, halogen, (C 3 -C 8 ) cycloalkyl, -C(0) " NR 21 R 22 , -S(0)2(Ci-C6) al kyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-C 6 ) alkoxy, OH, and heterocvcloalkyl.
  • Ri6 is (C1-C4) alkyl, (C1-C4) alkoxy,
  • R10 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) hydroxyalkyl, halogen,
  • (C 3 -C 8 ) cycloalkyl, or - R19R20 wherein the al kyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl.
  • Rie is (Ci-Gs) alkyl, (C3-Cs) cycloalkyl, -C(0)NR2iR22, ⁇ S(0)2(Ci-Ce) alkyl, -OH, or - R19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloal kyl .
  • Rie is (C1-C4) aikyi, (C 3 -Cs) cycloalkyl, or -NR19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl.
  • two Rie together when attached to the same atom form a (C 3 -C 8 ) spirocycloalkyl optionally substituted with one to three R23 when Re is
  • two Ri6 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one to three R23.
  • two Rie together when on adjacent atoms form a heteroaryi ring optionally substituted with one to three R23.
  • two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R 23 when Re is -(C0-C3) aikyiene-heterocycloaikyi or - N(R8)-(Co-C 3 ) alkylene-heterocycloalkyl ,
  • two Rie together when attached to the same carbon can form C (()) when R 6 is -(C0-C3) alkylene-heterocycloalkyl, -O -heterocycloalkyl, or
  • R 16 when attached to the same atom form a (Cs-Cg) spirocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -Q-heteroeycioalkyl, or
  • two Rie together when attached to the same atom form a (C 3 -Cs) spiroheterocycloal kyl optionally substituted with one or more R 23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycioalkyl, or
  • two Rie together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more R23.
  • two Rie together when on adjacent atoms form a heteroaryi ring optionally substituted with one or more R2.3.
  • two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
  • R17 i (Ci-Ci) alkyl, (C1-C4) aikoxy, (Ci-C4) haloalkvl, (C1-C4) haloaikoxy, halogen, cycioalkyl, heterocycloalkyl, or
  • alkyl (0-C 4 ) alkyl, (Ci-d) haloalkyl, halogen, heterocycloalkyl, or -C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Oj) aikoxy and -OH.
  • Ris is (C1-C4) alkyl, (C1-C4) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloaikoxy, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl.
  • Ri8 is (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl.
  • Ris is (C1-C4) alkyl, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In another embodiment, Ris is (C1-C4) alkyl, -OH, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In another embodiment, Ris is -C(0)OH, or
  • R1 is H, (C1-C4) alkyl
  • R19 is H, (C1-C4) alkyl, (C'i-Ce) cycloalkyl, -CH 2 C(0) H2, -S(0) 2 (Ci-C4) alkyl, -S(0) 2 (C6-Cio) aryl, or -C(0)(Ci-C 4 ) alkyl.
  • R19 is H, (Ci-Cs) alkyl or (C 3 -Ce) cycloal kyl .
  • R19 i s H (Ci-C 4 ) alkyl -CH2C(0)NH 2 , -S(0) 2 (Ci-C 4 ) alkyl, -S(0) 2 (C 6 -Cio) aryl, or -C(0)(Ci-C 4 ) alkyl.
  • R20 is H, (C1-C4) alkyl
  • R20 is H, (C1-C4) alkyl or (( ' ; ⁇ ( ' .-. ⁇ cycloalkyl.
  • R20 is H, (C1-C4) alkyl -CI W(0) ⁇ l . -S(0) 2 (Ci-C 4 ) alkyl, -S(0) 2 (Ce-Cio) aryl, or -C(0)(Ci-C 4 ) alkyl.
  • R21 i s H or (C1-C3) alkyl.
  • Rn is H, methyl, ethyl, n-propyi, or iso-propyl.
  • R22 is H or (C1-C3) alkyl . In another embodiment, R22 is H, methyl, ethyl, n-propyi, or iso-propyl .
  • R23 is (C1-C3) alkyl, (Ci-Cs) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxv, or halogen.
  • R23 is (C1-C2) alkyl, (Ci-C 2 ) alkoxy, (Ci-C 2 ) haloalkyl, (Ci-C 2 ) haloalkoxv, or halogen.
  • R23 is (C1-C2) alkyl, (C1-C2) haloalkyl, or halogen.
  • R23 is (Ci-C 2 ) alkyl, or (C1-C2) haloalkyl. In another embodiment, R23 is (C1-C3) alkyl. In another embodiment, R 23 is methyl, ethyl, n-propyl, or iso-propyl.
  • n is 0, 1, or 2. In another embodiment, n is 0 or I . In vet another embodiment, n is 1 , 2. or 3. In another embodiment, n is J or 2. In another embodiment, n is 2 or 3. In another embodiment, n is 0. In another embodiment, n is 1. In another embodiment, n is 2. In another embodiment, n is 3.
  • X is CH.
  • R 4 i s H or CH 3 and R? is H or CH 3 .
  • 4 is H or CH3, Rs is H or CH3, and Rs is H, ffuoro, or CH3.
  • R2 is H, (Ci-Ce) alkyl
  • Ri and R 2 together form a
  • R2 and R3 together form a
  • Ri is H, (Ci-C 4 ) alkyl, (Ci-C 4 ) alkoxy, (Ci-C 4 ) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C 3 -C 6 ) cycloalkvl, or - 9R10;
  • R2 is (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, (C3-C6) cycioalkyl, or ⁇ NR.11R.12; and
  • R3 is H, (C1-C3) alkyl, (C1-C3) alkoxy,
  • X is CR7.
  • Ri is H, (C1-C4) alkyl, (C i-C i) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
  • X is CR7
  • Ri is H, (C1-C4) alkyl , (C1-C4) al koxy, (Ci-Gs) haloalkyl, (C1-C4) haloalkoxy,
  • X is CR7
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
  • X is CR7
  • Ri is H, (d-d) alkyl, (Ci-d) alkoxy, (Ci-d) haloalkyl, (d-d-) haloalkoxy, (d-C 4 ) hydroxyalkyl, halogen,
  • (d-Cs) cycloalkyi, 01- --NR9R10, Ri is H, (C1-C4) alkyl, (d-d) haloalkyl, halogen,
  • R 3 is H, (d-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, and R 4 is H or (d-d) alkyl .
  • X is CR7
  • Ri is H, (C1-C4) alkyl
  • (d-d) cycloalkyi, or -NR9R10, R2 is H, (d-d) alkyl, (C1-C4) haloalkyl, halogen,
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (C1-C3) alkyl
  • R5 is H or (d-d) alkyl.
  • X is CR7
  • Ri is H, (d-d) alkyl, (Ci-Ci) alkoxy, (d-d) haloalkyl, (d -d) haloalkoxy, (d-C 4 ) hydroxyalkyl, halogen, (d-d) cycloalkyi, or -NR R10
  • R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyi, or -NR11R12
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (Ci-Cs) alkyl
  • Rs is H or (C1-C3) alkyl
  • Rs' is H or (Ci-d) alkyl.
  • X is CR7
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
  • R2 is H, (C1-C4) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyi, or - R11R12
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce.) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (C1-C3) alkyl
  • R 5 is H or (Ci- C3) alkyl
  • R.v is H or (C1-C3) alkyl
  • e is -(C0-C3) alkylene-heterocycloalkyl optionally substituted with one or more ie.
  • X is CR7, Ri i s H, (C1-C4) alkyl, (Ci-Ci) alkoxy,
  • R2 is H, (C1-C4) al kyl , (C1-C4) haloal kyl , halogen, (Cs-Cs) cycloalkyi, or -NRnR 12
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (C1-C3) alkyl
  • Rs is H or (Ci- C3) alkyl
  • Rs' is H or (C1-C3) alkyl
  • Re is -O-heterocycloalkyl optionally substituted with one or more Rie.
  • X is CR7
  • Ri is H
  • (Ci-d) alkoxy
  • R2 is H, (Ci-d) alkyl, (C1-C4) haloalkyl, halogen, (d-d) cycloalkyi, or -NRiiRi?.
  • R3 is H, (Ci-Ce) alkyl, (C1-C0) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (d-d) alkyl
  • Rs is I I or (C1-C3) alkyl
  • Rs- is H or (d-d) alkyl
  • Re is -(C0-C3) alkylene-C(0)OH.
  • X is CR?
  • Ri is H, (d-d) alkyl, (d-d) alkoxy, (d-d) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10
  • R.2 is H, (d-d) al ky] , (C1-C4) haloal kyl , halogen, (d-d) cycloalkyl, or -NRiiRi2
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (d -d) haloalkyl
  • R 4 is H or (Ci-d) alkyl
  • Rs is H or (C1-C3) alkyl
  • Re is -(C0-C3) alkylene
  • X is CR7
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
  • R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (d-d) cycloalkyl, or -NR11R12
  • R3 is H, (d-d) alkyl, (d-d) alkoxy, (d-d) haloalkyl
  • Rs is H or (d-d) alkyl
  • R5 is H or (d-d) alkyl
  • R.v is H or (d-d) alkyl
  • Re is -N(Rs)-(Co-d) alkylene-heterocycloaikyi optionally substituted with one or more Rie.
  • X is CR7
  • Ri is H
  • (d-d) alkyl (d-d) alkoxy
  • R2 is H, (C1-C4) alkyl, (C i-d ) haloalkyl, halogen, (C3-Cs) cycloalkyl, or -NR11R12
  • R3 is H, (Ci-Ce) alkyl, (d-d) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (C1-C3) alkyl
  • R 5 is H or (Ci- C3) alkyl
  • Rs 1 is H or (d-d) alkyl
  • Re is -(Co-d) alkylene-aryl optionally substituted with one or more Rie.
  • X is CR7
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
  • (d-d) haloalkyl, (d-d) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, R?. is H, (C1-C4) alkyl, (Ci-d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or --NR11R12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R 4 is H or (d-d) alkyl, R5 is H or (C1-C3) alkyl, y is H or (C1-C3) alkyl, and Re and R7 together when on adjacent atoms form a (d-d) cycloalkyl ring optionally substituted with one or more Rig.
  • X is CR7
  • Ri is H
  • (d-d) alkyl (d-d) alkoxy
  • X is N. In another embodiment, X is N.
  • Ri is H, (d-d) alkyl, (CJ -C 4 ) alkoxy, (C1-C4) haloalkyl, (Ci-C 4 ) haloalkoxy,
  • X is N
  • Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or -NR9R10
  • II2 is H, (d-d) alkyl, (d-d) haloalkyl, halogen,
  • X is N
  • Ri is H, (d-d) alkyl, (d-d) alkoxy, (C1-C4) haloalkyl,
  • X is N
  • Ri is H
  • X is N
  • Ri is H
  • R2 is H, (d-d) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or - R11R12, R? is H, (Ci-Ce) alkyl, (d-d.) alkoxy, (Ci-Ce) haloalkyl, R 4 i s H or (C1-C3) alkyl, R 5 is H or
  • X is N
  • Ri is H
  • (d-d) alkyl (d-d) alkoxy
  • R2 is H, (d-d) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or - R11R12, R?
  • R 5 is H or (C 1-C3) alkyl
  • Rs 1 is H or (d-d) alkyl
  • Re is -(C0-C3) alkylene-heterocycioalkyl optionally substituted with one or more Ri6.
  • X is N
  • Ri is H
  • (d-d) alkyl (d-d) alkoxy
  • (Ci-d) haloalkyl, (d-d) haloalkoxy, (Ci-d) hydroxyalkyl, halogen, (C 3 -Cs) cycloalkyl, or -NR9R10, R?. is H, (C1-C4) alkyl, (d-d) haloalkyl, halogen, (d -d) cycloalkyi, or -NR11R12, R?
  • R 4 is H or (C1-C3) alkyl
  • R5 is H or (d-d) al ky]
  • R 5 ' is H or (C1-C3) al kyl
  • Re is -O-heterocycloalkyl optionally substituted with
  • X is N
  • Ri is H
  • X is N
  • Ri is H
  • R5 is H or (d-d) alkyl
  • Rs- is H or (C1-C3) alkyl
  • Re is -(C0-C3) alkyl ene-heteroaryl optionally substituted with one or more Rie.
  • X is N
  • Ri is H
  • (d-d) alkyl is H
  • R2 is H, (d-d) al kyl , (C1-C4) haloal kyl , halogen, (d-d) cycloalkyi, or -NRiiRi2
  • R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl
  • R 4 is H or (Ci-Cs) alkyl
  • Rs is H or (C1-C3) alkyl
  • R5' is H or (C1-C3) alkyl
  • Re is -N(Rs)-(Co-C 3 ) alkylene-heterocycloalkyl optionally substituted with one or more
  • X is N
  • Ri is H
  • R2 is H, (C i -C i ) alkyl, (C1-C4) haloalkyl, halogen, (Cs-Cg) cycloalkyi, or -NRiiRi?.
  • R3 is It (d-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R3 ⁇ 4 is H or (Ci-C 3 ) alkyl
  • Rs is H or
  • X is N
  • Ri is H
  • (d-C 4 ) alkyl (d-C 4 ) alkoxy
  • R2 is H, (C1-C4) alkyl, (Ci-C ) haloalkyl, halogen, (Cs-Cs) cycloalkyl, or ⁇ NRnRi2
  • Rs is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (C1-C0) haloalkyl
  • R 4 is H or (C1-C3) alkyl
  • R5 is H or (C1-C3) alkyl
  • Ry is H or (C1-C3) alkyl
  • Re and R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring optionally substituted with one or more Ris.
  • X is N
  • Ri is H
  • Ri is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (Ci-Cs) cycloalkyl, or -NRnRi2, R?
  • R is H, (C1-C0) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl
  • R is H or (C1-C3) alkyl
  • R5 is H or (C1-C3) alkyl
  • R 5 ' is H or (C1-C3) alkyl
  • Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris
  • Non-limiting illustrative compounds of the disclosure include:
  • the compounds of Formula (I) are enantiomers. In some embodiments the compounds are the ( ⁇ -enantiomer. In other embodiments the compounds are the (R)-enantiomer. In yet other embodiments, the compounds of Formula (I) may be (+) or (-) enantiomers.
  • the compounds of the disclosure may contain asymmetric or chirai centers, and, therefore, exist in different stereoisomenc forms. It is intended that all stereoisomers forms of the compounds of the disclosure as well as mixtures thereof, including racemic mixtures, form part of the present disclosure.
  • the present disclosure embraces all geometric and positional isomers. For example, if a compound of the disclosure incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the disclosure.
  • Each compound herein disclosed includes all the enantiomers that conform to the general structure of the compound.
  • the compounds may be in a racemic or enantiomerically pure form, or any other form in terms of stereochemistry.
  • the assay results may reflect the data collected for the racemic form, the enantiomerically pure form, or any other form in terms of stereochemistry.
  • Diastereomeiic mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and/or fractional crystallization.
  • Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chirai auxiliary such as a chirai alcohol or Mosher's acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the optically active compound
  • an appropriate optically active compound e.g., chirai auxiliary such as a chirai alcohol or Mosher's acid chloride
  • the compounds of the disclosure may exist in different tautomeric forms, and ail such forms are embraced within the scope of the disclosure. Also, for example, all keto-enol and imine-enamine forms of the compounds are included in the disclosure.
  • All stereoisomers for example, geometric isomers, optical isomers and the like
  • of the present compounds including those of the salts, solvates, esters and prodrugs of the compounds as well as the salts, solvates and esters of the prodrugs
  • those which may exist due to asymmetric carbons on various substituents including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this disclosure, as are positional isomers (such as, for example, 4-pyridyl and 3-pyridyl).
  • salt is intended to equally apply to the salt, solvate, ester and prodrug of enantioniers, stereoisomers, rotamers, tautomers, positional isomers, racemates or prodrugs of the inventive compounds.
  • the compounds of Formula I may form salts which are also within the scope of this disclosure.
  • Reference to a compound of the Formula herein is understood to include reference to salts thereof, unless otherwise indicated.
  • the present disclosure relates to compounds which are modulators of USP28 and/or USP25.
  • the compounds of the present disclosure are inhibitors of USP28 and/or USP25.
  • the disclosure is directed to compounds as described herein and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, and pharmaceutical compositions comprising one or more compounds as described herein, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof.
  • the compounds of Formula (I) may be prepared by methods known in the art of organic synthesis as set forth in part by the following synthetic schemes. In the schemes described below, it is well understood that protecting groups for sensitive or reactive groups are employed where necessary in accordance with general principles or chemistry. Protecting groups are manipulated according to standard methods of organic synthesis (T. W. Greene and P. G. M. Wuts, "Protective Groups in Organic Synthesis", Third edition, Wiley, New York 1999). These groups are removed at a convenient stage of the compound synthesis using methods that are readily apparent to those skilled in the art. The selection processes, as well as the reaction conditions and order of their execution, shall be consistent with the preparation of compounds of Formula (I).
  • the compounds described herein may be made from commercially available starting materials or synthesized using known organic, inorganic, and/or enzymatic processes.
  • the compounds of the present invention can be prepared in a number of ways well known to those skilled in the art of organic synthesis.
  • compounds of the present invention can be synthesized using the methods described below, together with synthetic methods known in the art of synthetic organic chemistry, or variations thereof as appreciated by those skilled in the art. Preferred methods include but are not limited to those methods described below.
  • Compounds of the present invention can be synthesized by following the steps outlined in General Schemes 1 and 2 which comprise different sequences of assembling intermediates 2a, 2b, 2c, 2d, and It. Starting materials are either commercially available or made by known procedures in the reported literature or as illustrated.
  • R1-R5, R5 1 , Re, Rr, m, and n are defined as in Formula (I).
  • l-Ethyl-3- (3 -dimethyl aminopropy 1 jcarbodiimide with 1 -hydroxybenzotri azole (EDC I/HOB t), (Benzotriazol-l-yloxy)tris(dimethylamino)phosphonium hexafiuorophosphate (BOP), O- benzotriazole-N, ' N, ' N ; N'-tetrarnethyl-uronium-hexaf!.uoro-phosphate (HBTU) or [bis (dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-b]pyridinium-3-oxide hexafiuorophosphate (HATU), and a base, e.g., triethylamine (TEA). ⁇ , ⁇ -diisopropylethylamine (DIEA), or 4- dimethylaminopyridine (DMAP), in a solvent
  • R1-R5, Rs; Re, R?, m, and n are defined as in Formula (I).
  • compounds of Formula (I) can also be prepared by using intermediates 2b, 2c, 2d, and 2e as depicted in General Scheme 2 above.
  • a base e.g.. triethylamine (TEA) or ⁇ , ⁇ -diisopropylethylamine (DIPEA)
  • DIPEA ⁇ , ⁇ -diisopropylethylamine
  • a solvent e.g., DCM or THF
  • Compounds of Formula (I) can exist as enantiomeric or diastereomeric stereoisomers.
  • Enantionierically pure compounds of Formula (I) can be prepared using enantionierically pure chiral building blocks.
  • racemic mixtures of the final compounds or a racemic mixture of an advanced intermediate can be subjected to chiral purification as described herein below to deliver the desired enantionierically pure intermediates or final compounds.
  • each individual enantiomer can be carried on separately to deliver the final enantionierically pure compounds of Formula (I).
  • Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP28.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 an effective amount the compositions and compounds of Formula (I).
  • the disease or disorder is cancer.
  • the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 an effective amount the compositions and compounds of Formula (I).
  • the disease or disorder is cancer.
  • the present disclosure is directed to a method of inhibiting USP28.
  • the method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
  • Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP25.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP25 an effective amount the compositions and compounds of Formula (I).
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP25 an effective amount the compositions and compounds of
  • the disease or disorder is cancer. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
  • the present disclosure is directed to a method of inhibiting USP25, The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
  • Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP25.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 and USP25 an effective amount the compositions and compounds of Formula (I).
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28.
  • the method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 and USP25 an effective amount the compositions and compounds of Formula (I).
  • the disease or disorder is cancer.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present disclosure is directed to a method of inhibiting USP28 and USP25.
  • the method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
  • Another aspect of the present di sclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of
  • the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I), in one embodiment, the disease or disorder is cancer,
  • Another aspect of the present disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of USP25, the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I), in one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
  • Another aspect of the present disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of USP28 and USP25, the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I).
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present invention relates to a method of treating, preventing, inhibiting, or eliminating cancer.
  • the method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
  • the present invention relates to a method of treating, preventing, inhibiting, or eliminating inflammation.
  • the method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • the present invention relates to a method of treating, preventing, inhibiting, or eliminating an autoimmune disease.
  • the method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
  • the present invention in another aspect relates to a method of treating, preventing, inhibiting, or eliminating an infectious disease, The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • the infectious disease is a viral infection.
  • the infectious disease is a bacterial infection.
  • the present invention relates to a method of treating, preventing, inhibiting, or eliminating a viral infection.
  • the method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof,
  • the present invention relates to a method of treating, preventing, inhibiting, or eliminating a bacterial infection.
  • the method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
  • Another aspect of the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28.
  • the disease or disorder is cancer.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP25.
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation,
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • Another aspect of the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28 and USP25.
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating cancer.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating inflammation.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating an autoimmune disease.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating an infectious disease.
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating a viral infection,
  • the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating a bacterial infection.
  • Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28.
  • the disease or disorder is cancer
  • Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28 and USP25.
  • the disease or disorder is cancer.
  • the disease or disorder is inflammation.
  • the disease or disorder is an autoimmune disease.
  • the disease or disorder is an infectious disease.
  • the disease or disorder is a viral infection.
  • the disease or disorder is a bacterial infection.
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating cancer.
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating inflammation,
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating autoimmune disorder.
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating an
  • the infectious disease is a viral infection. In another embodiment, the infectious disease is a bacterial infection,
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a viral infection.
  • the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a bacterial infection.
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with cancer.
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with inflammation,
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with an autoimmune disease.
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with an infectious disease
  • the infectious disease is a viral infection.
  • the infectious disease is a bacterial infection.
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with a viral infection.
  • the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with a bacterial infection.
  • the present disclosure also relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP28, wherein the medicament comprises a compound of Formula (I).
  • the present disclosure also relates to the use of an inhibitor of USP25 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP25, wherein the medicament comprises a compound of Formula (1),
  • the present disclosure also relates to the use of an inhibitor of USP28 and USP25 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP28 and USP25, wherein the medicament comprises a compound of Formula (I).
  • the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP28, wherein the medicament comprises a compound of Formula (I),
  • Another aspect of the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP25, wherein the medicament comprises a compound of Formula (I).
  • the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP28 and USP25, wherein the medicament comprises a compound of Formula (i).
  • the cancer is selected from bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g., squamous cell carcinoma), colorectal cancer (e.g., adenocarcinoma), colon cancer, esophageal cancer (e.g., squamous cell carcinoma), gastric cancer (e.g., adenocarcinoma, choriocarcinoma, squamous cell carcinoma), head and neck cancer, hematologic cancer (e.g., acute lymphocytic anemia, acute
  • vl myeloid leukemia acute lymphoblastic B ceil leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt's lymphoma, chronic lymphocytic leukemia, chronic eosinophillic ieukemia/hypereosinophiliic syndrome, chronic myeloid leukemia, Hodgkin's lymphoma, mantle cell lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchi oloalveolar adenocarcinoma, mesothelioma, mucoepidermoid carcinoma, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma, squamous cell carcinoma), liver cancer (e.g., hepatocellular carcinoma), lymphoma, neurological cancer (e.g., glioblastoma, neuroblastoma, neuroblastoma
  • the cancer is a cancer that is sensitive to USP28 inhibition
  • the cancer in other embodments, is a cancer that is sensitive to USP25 inhibition. In other embodments, the cancer is a cancer that is sensitive to USP28 and USP25 inhibition.
  • the cancer can be any cancer in any organ, for example, a cancer is selected from the group consisting of glioma, thyroid carcinoma, breast carcinoma, small-cell lung carcinoma, non-small-cell carcinoma, gastric carcinoma, colon carcinoma, gastrointestinal stromal carcinoma, pancreatic carcinoma, bile duct carcinoma, CNS carcinoma, ovarian carcinoma, endometrial carcinoma, prostate carcinoma, renal carcinoma, anaplastic large-cell lymphoma, leukemia, multiple myeloma, mesothelioma, and melanoma, and combinations thereof
  • the cancer is selected from acute myeloid leukemia, gastric, pancreatic, colorectal, glioblastoma, neuroblastoma, small -ceil lung, non-small ceil lung, and squamous cell carcinoma.
  • the present disclosure relates to a compound of Formula (I) or a pharmaceutical composition comprising a compound of the present disclosure and a pharmaceutically acceptable carrier used for the treatment of cancers including, but not limited to, bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g., squamous cell
  • colorectal cancer e.g., adenocarcinoma
  • esophageal cancer e.g., squamous cell carcinoma
  • gastric cancer e.g., adenocarcinoma, choriocarcinoma, squamous cell carcinoma
  • head and neck cancer hematologic cancer (e.g., acute lymphocytic anemia, acute myeloid leukemia, acute lymphoblastic B cell leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt's lymphoma, chronic lymphocytic leukemia, chronic eosinophillic ieukemia/hypereosinophiliic syndrome, chronic myeloid leukemia, Hodgkin's lymphoma, mantle cell lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchioloalveolar
  • the cancer is selected from acute myeloid leukemia, gastric cancer, pancreatic cancer, colorectal cancer, glioblastoma, neuroblastoma, small-cell lung cancer, non-small cell lung cancer, and squamous cell carcinoma,
  • the patient is selected for treatment based on gene amplification and/or elevated tumor expression of USP28, MYC, LSD1, NICDl, and/or reduced expression of FBXW7 relative to tissue-matched expression.
  • the patient is selected for treatment based on gene amplification and/or elevated tumor expression of USP28, USP25, MYC, LSD1, NICDl, and/or reduced expression of FBXW7 relative to tissue-matched expression.
  • administration of a compound of Formula (I) or a pharmaceutical composition comprising a compound of the present disclosure and a pharmaceutically acceptable carrier induces a change in the cell cycle, ceil viability, cell apoptosis, or differentiation.
  • the change in the ceil cycle or cell viability or differentiation may be indicated by decreased tumor levels of MYC, LSD1, NICDl, PIM1, CDK1, POLA2, HEY1, and/or CCND1 , and/or increased levels of CD86 , p21, LGALS4, and/or DLL! .
  • the present disclosure relates to a compound of Formula (I) or a pharmaceutical composition
  • a pharmaceutically acceptable carrier used for the treatmeni of autoimmune diseases including, but not limited to, multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto'
  • Gui n-Barre' syndrome Grave's disease, myasthenia gravis, Sjogren's syndrome, nodular panarteritis, autoimmune enteropathy, uveitis, autoimmune oophoritis, chronic immune thrombocytopenic purpura, colitis, diabetes, psoriasis, pemphigus vulgaris, proliferative glomerulonephritis, Wiskott-Aldrich syndrome, autoimmune lymphoproliferative syndrome, chronic arthritis, inflammatory chronic rhinosinusitis, colitis, celiac disease, inflammatory bowel disease, Barrett's esophagus, inflammatory gastritis, autoimmune nephritis, autoimmune vasculitis, autoimmune hepatitis, autoimmune carditis, autoimmune encephalitis, and autoimmune mediated hematological disease.
  • the autoimmune disease can be, for example, an autoimmune disease selected from multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto's disease, Guillain-Barre' syndrome, Grave's disease, myasthenia gravis, Sjogren
  • compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier.
  • the pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.
  • a disease or disorder associated with modulation of USP28 including cancer comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
  • a disease or disorder associated with modulation of USP25 including cancer, inflammation, an autoimmune disease, a viral infection and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
  • a disease or disorder associated with modulation of USP28 and USP25 including cancer, inflammation, an autoimmune disease, a viral infection and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
  • One therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 is to provide treatment to patients or subjects suffering from cancer.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from cancer.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from cancer.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from inflammation.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from inflammation.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from an autoimmune disease.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from an autoimmune disease.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from an infectious disease.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from an infectious disease.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from a viral infection.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from a viral infection.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from a bacterial infection.
  • Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from a bacterial infection.
  • the disclosed compounds of the disclosure can be administered in effective amounts to treat or prevent a disorder and/or prevent the development thereof in subjects.
  • Administration of the disclosed compounds can be accomplished via any mode of administration for therapeutic agents, These modes include systemic or local administration such as
  • compositions can be in solid, semi-solid or liquid dosage form, such as, for example, injectables, tablets, suppositories, pills, time-release capsules, elixirs, tinctures, emulsions, syrups, powders, liquids, suspensions, or the like, sometimes in unit dosages and consistent with conventional pharmaceutical practices.
  • injectables tablets, suppositories, pills, time-release capsules, elixirs, tinctures, emulsions, syrups, powders, liquids, suspensions, or the like, sometimes in unit dosages and consistent with conventional pharmaceutical practices.
  • they can also be administered in intravenous (both bolus and infusion), intraperitoneal, subcutaneous or intramuscular form, and ail using forms well known to those skilled in the pharmaceutical arts.
  • Illustrative pharmaceutical compositions are tablets and gelatin capsules comprising a Compound of the Disclosure and a pharmaceutically acceptable carrier, such as a) a diluent, e.g., purified water, triglyceride oils, such as hydrogenated or partially hydrogenated vegetable oil, or mixtures thereof, corn oil, olive oil, sunflower oil, safflower oil, fish oils, such as EPA or DHA, or their esters or triglycerides or mixtures thereof, omega-3 fatty acids or derivatives thereof, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, sodium, saccharin, glucose and/or glycine; b) a lubricant, e.g., silica, talcum, stearic acid, its magnesium or calcium salt, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and/or polyethylene glycol; for example, purified
  • Liquid, particularly injectable, compositions can, for example, be prepared by dissolution, dispersion, etc.
  • the disclosed compound is dissolved in or mixed with a pharmaceutically acceptable solvent such as, for example, water, saline, aqueous dextrose,
  • Proteins such as albumin, chylomicron particles, or serum proteins can be used to solubilize the disclosed compounds,
  • the disclosed compounds can be also formulated as a suppository that can be prepared from fatty emulsions or suspensions; using polyaikylene glycols such as propylene glycol, as the carrier.
  • the disclosed compounds can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles.
  • Liposomes can be formed from a variety of phospholipids, containing cholesterol, stearyl amine or phosphatidylcholines.
  • a film of lipid components is hydrated with an aqueous solution of drug to a form lipid layer encapsulating the drug, as described in U.S. Pat. No. 5,262,564 which is hereby incorporated by reference in its entirety.
  • Disclosed compounds can also be delivered by the use of monoclonal antibodies as individual carriers to which the disclosed compounds are coupled.
  • the disclosed compounds can also be coupled with soluble polymers as targetable drug carriers.
  • Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxyethylaspanamidephenol, or polyethyleneoxidepolylysine substituted with palmitoyl residues.
  • Disclosed compounds can be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polyiactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross-linked or amphipathic block copolymers of hydrogels.
  • a polymer e.g., a polycarboxylic acid polymer, or a polyacrylate.
  • Parental injectable administration is generally used for subcutaneous, intramuscular or intravenous injections and infusions.
  • Injectables can be prepared in conventional forms, either as liquid solutions or suspensions or solid forms suitable for dissolving in liquid prior to injection.
  • compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier.
  • the pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.
  • compositions can be prepared according to conventional mixing, granulating or coating methods, respectively, and the present pharmaceutical compositions can contain from about 0.1% to about 99%, from about 5% to about 90%, or from about 1% to about 20% of the disclosed compound by weight or volume.
  • the dosage regimen utilizing the disclosed compound is selected in accordance with a variety of factors including type, species, age, weight, sex and medical condition of the patient; the severity of the condition to be treated, the route of administration; the renal or hepatic function of the patient, and the particular disclosed compound employed.
  • a physician or veterinarian of ordinary skill in the art can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
  • Effective dosage amounts of the disclosed compounds when used for the indicated effects, range from about 0.5 mg to about 5000 mg of the disclosed compound as needed to treat the condition.
  • Compositions for in vivo or in vitro use can contain about 0,5, 5, 20, 50, 75, 100, 150, 250, 500, 750, 1000, 1250, 2500, 3500, or 5000 mg of the disclosed compound, or, in a range of from one amount to another amount in the list of doses.
  • the compositions are in the form of a tablet that can be scored.
  • RuPhos Pd 2nd Gen Chloro(2-dicyclohexylphosphino-2',6'-diisopropoxy-l,r-biphenyl)[2-(2'- amino-1, 1 '-biphenyl)]palladium(II) s singlet
  • Xphos 2nd generation precataiyst Chloro(2-dicyclohexylphosphino-2',4',6'-triisopropyl-l, r- biphenyl)[2-(2'-amino-l, 1 '-biphenyl)]palladium(II)
  • Step 3 2 ⁇ C loro ⁇ 5 ⁇ (diflnoromethy!)nieotiiiO!iitriIe
  • the caide product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :3) to afford 2- chloro-5-fluoro-6-methoxynicotinonitrile as a yellow solid (2,9 g) that was carried on without further purification.
  • LCMS ( LSI, ;,v .:): 187
  • Step 1 2 ⁇ ChIoro ⁇ 6 ⁇ ethyI-5 ⁇ fl oroeicotiiiOiiitriIe
  • Step 2 Benzyl 4-(4-(2-((1 ⁇ 2/ -butoxycarbonyl)amino)ethyl)phenyl)piperazine-l-carboxylate
  • reaction mixture was stirred overnight at 105 °C in an oil bath and then cooled to RT and quenched with H2O (200 mL). The resulting mixture was extracted with ethyl acetate (2 x 50 mL). The combined organic layers were washed with brine (1 x 200 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo.

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Abstract

The disclosure relates to inhibitors of USP28 and/or USP25 useful in the treatment of cancers, inflammation, autoimmune diseases, and infectious diseases, having the Formula (I), where R1, R2, R3, R4, R5, R5', R6, R7, X, m, and n are described herein.

Description

[0001] This application claims the benefit of and priority to U.S. provisional application No. 62/294,625, filed February 12, 2016, the entire contents of which are incorporated herein by reference in its entirety.
Field of Invention
[0002] The present disclosure is directed to modulators of ubiquitin-specific protease 28 (USP28) and/or ubiquitin-specific protease 25 (USP25) useful in the treatment of diseases or disorders associated with USP28 and/or USP25 enzymes. Specifically, the disclosure is concerned with compounds and compositions inhibiting USP28 and/or USP25, methods of treating diseases or disorders associated with USP28 and/or USP25, and methods of synthesis of these compounds.
Figure imgf000002_0001
[0003] USP28 and USP25 are cysteine isopeptidases of the USP sub-family of DUBs containing three distinct domains: an N-terminal UBA-like domain; a pair of ubiquitin- interacting motifs (UIM) and a USP domain that is predicted to have the conserved fold of the USP sub-family (Nijman et ai., Cell 2005, 123, 773-786; Komander et al., Mol. Cell Bio. 2009, 10, 550-563). USP28 and USP25 exert their function through regulating the stability of a plethora of cellular proteins. USP28 has been characterized as a tumor-promoting factor and has been found to stabilize many oncoproteins. USP25 has been characterized as a tumor-promoting factor and as a regulator of cellular responses related to autoimmune disease, inflammation, and infectious diseases (such as viruses and bacteria).
[0004] Amplification, deletions and mutations of USP28 have been identified in multiple cancer types, including breast cancer, AML, ovarian cancer, and colorectal cancer, (cbioportal; http://www.cbioportal.org; Diefenbacher et al., J. of Clin. Investi. 2014, 124, 3407-3418; Popov et al ., Nat. Cell. Biol 2007, 9, 729-731 ). Furthermore, USP28 overexpression has been correlated with poor prognosis in patients with glioblastoma, non-small ceil lung carcinoma and bladder cancers suggesting that USP28 plays an important role in tumorigenesis of these tumor types. (Wang et al., Exp. Biol. Med. 2016, 255-264; Zhang et al., J. Cell. Mol. Med. 2015, 19, 799-805; Guo et al., Tumor Bio. 2014, 35, 4017-4022).
[0005] A large-scale shRNA screen has also identified a role of USP28 in the control of the stability of MYC protein. (Popov, Nat. Cell. Biol, 765-774). MYC is a master regulator of the transcription of genes involved in cell growth, proliferation and apoptosis and is essential for tumor initiation and maintenance in many tumor types. (Meyer et al., Nat. Rev. Cancer 2008, 8, 976-990; Conacci-Sorrell et al., Cold Spring Harh. Perspect. Med, 2014, 4, 1-24; Huang et al., Cold Spring Harb. Perspect. Med. 2013; Roussel et al., Cold Spring Harh. Perspect. Med. 2013, Gabay et al., Cold Spring Harb. Perspect. Med. 2014; Schmitz et al., Cold Spring Harb. Perspect. Med. 2014). In addition, MYC is the most frequently amplified oncogene in human cancer, with alterations in many tumor types including breast, lung and prostate. (Beroukhim et al. Nature 2010, 463, 899-905). Knockdown of the USP28 gene has been shown to lead to a decrease of M YC protein and an associated inhibition of growth in a panel of human cancer cell lines in vitro. (Popov, Nat. Cell Biol., 765-774).
[0006] USP28 has also been reported to be required to impart stability on the LSDl (ly sine- specific demethylase 1) protein. (Wu et al., Cell Rep. 2013, J, 224-236). LSDl is a histone demethylase that complexes with many partner proteins to control cellular pluripotency and differentiation. (Metzger et al. Nature 2005, 437, 436-439; Toffolo et al, J. Neurochem. 2014 128, 603-616, 2014; Peri / et al,, PloS Biology; 2015). Knockdown of USP28 in tumor cells has been shown to lead to the destabilization of LSDl protein, the suppression of cancer stem cell (CSC)-like characteristics in vitro, and the inhibition of tumor growth in vivo. (Wu, Cell Rep., 224-236). Small molecule in hibitors of LSDl have shown antitumor activity in models of AML and Ewing sarcoma. (Sankar et al., Clin Cancer Res. 2014 4584-4597; Schenk et al., Nat. Med. 2012, 18, 605-611). Thus, USP28 inhibition represents an alternate approach to targeting LSDl in these tumor types.
[0007] USP28 inhibition has also been shown to reduce ICDl -Levels and to lead to inhibition of the NOTCH pathway activity. (Diefenbacher et al.). NOTCH signaling controls diverse cellular differentiation decisions and drives tumorigenesis in certain tumor types. NOTCH 1 is a potent T-cell oncogene, with >50% of T-cell acute lymphoblastic leukemia (T- ALL) cases carrying activating mutations in NOTCH ! . (Weng et al. Science 2004, 306, 269- 271). Increased NOTCH! protein levels have also been associated with disease progression in colon cancer. (Meng et al., Cancer Res. 2009, 69, 573-582). NOTCH 1 rearrangements lead to constitutive pathway activation and drive tumorigenesis in many cancer types, including triple- negative breast cancer. (Stoeck et al., Cancer Discov. 2014, 4, 1 154-1167).
[0008] Other reported substrates of USP28 include c-Jun, Cyclin E, HIF-la, Claspin, 53BP1, and Mdc l , many of which play important roles in tumorigenesis in humans. (Diefenbacher et al., Fiugel et al. Blood 2012, 119, 1292-1301; Zhang et al., Cell 2006, 126, 529-542). Interestingly, many USP28 substrates are recognized by FBW7, the substrate recognition subunit of SCF (FBW7) E3 ubiquitin ligase. (Diefenbacher et al). FBW7 recognizes USP28 substrates in a phosphorylation-dependent manner and targets them for ubiquitination ultimately leading to their proteasomal degradation. The antagonizing roles of USP28 and FBW7 on their shared oncoprotein substrates indicate the intricate nature of protein stability control and may provide additional therapeutic opportunities for cancer treatment.
[0009] Mice with a germline knockout of USP28 have been shown to be viable and fertile, confirming that USP28 activity is not required for normal development and reproductive function. (Knobel et al.. Molecular and Cellular Biology 2014, 34, 2062-2074). Conditional knockout of USP28 in mouse intestine led to the reduction of oncoproteins including c-Myc, active NOTCH (NICDl) and c-JUN which was associated with decreased intestinal cell proliferation and enhanced differentiation. More importantly, intestinal tumorigenesis induced by APC mutation was effectively blocked with acute USP28 depletion suggesting that USP28 could be an appealing target to reduce tumor burden and improve survival for intestinal cancers. (Diefenbacher et al.).
[0010] Mice with a germline knockout of USP25 have been shown to be viable and did not show any abnormalities in growth and survival (Zhong et al. Nat. Immunol. 2012, 13, 1110- 1117). Deficiency of USP25 in these mice led to increased inflammation and autoimmune responses mediated by interleukin 1 7 (IL-17), a pro-inflammatory cytokine that is a known regulator of host defense to infection, autoimmunity, and tumorigenesis (Zhong et al. 2012; Iwakura et al., Immunity 2011, 34, 149-162). As such, USP25 was characterized as a negative regulator of IL-17 signaling through its deubiquitinase activity on the tumor necrosis factor receptor-associated 5 (TRAF5) and TRAF6 adapter proteins associated with the IL-17 receptor complex.
[0011] USP25 has also been shown to be a negative regulator of innate immune responses activated by pathogens such as viruses and bacteria. USP25 has been shown to regulate the type I interferon (IFN) signaling pathway via its deubiquitinase activity on RIG-I, TRAP2, and TRAF6 and Toll-like receptor 4 (TLR-4) signaling via its deubiquitinase activity on TRAF3 (Zhong et al., PLOS One 2013, 8, e80976; Zhong et al. Sci. Signal. 2013, 6, ra35). Since the TRAF- associated family of adapter proteins have wide specificities for other cell surface receptor complexes (e.g., IL-17 receptor, TNF receptor, T cell receptor, ΤΟΡβ receptor, the Toll like receptor family, etc.), USP25 is likely to be a pleiotropic regulator of immune and inflammatory signaling in multiple disease contexts, including cancers (Walsh et al., Immunol. Rev. 2015, I, 72-92; Cui et al., Hum. Vaccin. Immunother.2014, 10, 3270-3285; Bradley and Pober, Oncogene 2001, 20, 6482-6491).
[0012] USP25 has been reported to promote tumor invasion and metastasis (Li et al., Mol. Cell 2014, 13, 166-170). Decreased expression of USP25 in lung cancer cell lines has been shown to decrease invasion and motility in in vitro experiments and to decrease tumor metastasis in in vivo experiments in mice. Studies have shown USP25 activity abrogated via expression of miRNA-200c which down-regulates USP25 expression. Additionally, analysis of USP25 and miRNA-200c expression levels in tissue from lung cancer patients revealed expression profiles consistent with USP25-driven tumorigenesis (i.e., elevated levels of USP25 and decreased levels of miRNA-200c in tumor tissue versus healthy tissue increased metastatic lesions, and poor clinical prognosis in patients with elevated tumor levels of USP25).
[0013] In summary, USP28 and IJSP25 play important roles in promoting tumorigenesis in ceils and modulating immune responses. Its major role being in the deubiquitination and stabilization of diverse oncoproteins and epigenetic drivers and immunomodulatory proteins among other cellular factors, which are necessary for immune responses and tumor initiation and growth in humans. Inhibition of USP28 and/or USP25 with small molecule inhibitors therefore has the potential to be a treatment for cancers, autoimmune diseases, inflammatory diseases, infectious diseases, and other disorders. For this reason, there remains a considerable need for novel and potent small molecule inhibitors of USP28 and/or USP25. Summary of the Disclosir
A first aspect of the disclosure relates to compounds of Formula (I):
Figure imgf000006_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof, wherein:
X is N or CR -;
Ri is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkvl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloaikyi, -CN, or -NRgRjo:
R2 is H, (Ci-C6) alkyl, (Cj -Ce) alkoxy, (Ci-Ce) haloalkvl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (Cs-Cs) cvcloalkyl, or -NR11R12;
R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-C6) haloaikyi, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloaikyi, or -NR13R14;
wherein at least one of Ri, R2, or R3 is not H; or Ri and R2 together form a (( VC 'H) cycloaikyi optionally substituted with one or more
Rl5, or R2 and R3 together form a (C-i-Cs) cycloaikyi optionally substituted with one or more
R15;
R4 is H, (Ci-Ce) alkyl, or (Ci-Ce) haloaikyi;
R5 is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloaikyi,
R5' i s H, (Ci-C6) alkyl, halogen, or (Ci-Ce) haloaikyi ; or R.4 and 5 together with the carbon atoms to which they are attached form a (C3-Cs) cycloalkyl ring;
Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
-O-heterocycloalkyl, -(C0-C3) alkylene-aryl, -(C0-C3) alkylene-heteroaryl or
-N(R-?)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl, aiyl and heteroaryl are optionally substituted with one or more Rie;
each R'/ is independently at each occurrence H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, (C3-Cs) cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, wherein the alkyl is optionally substituted with one or more (Ci-Ce) alkoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more Rn; or
Re and R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring optionally substituted with one or more Ris; or Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris; Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one or more Ris; or Re and R? together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more Ris, or two R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring; or two R? together when on adjacent atoms form a heterocycloalkyl ring; two R? together when on adjacent atoms form an aryl ring, or two R? together when on adjacent atoms form a heteroaryl ring;
Rs is H or (Ci-Ce) alkyl; each R9, Rio, Rn, R12, R13, and Ri4 is independently H, (Ci-Ce) alkyl, or
-C(0)(Ci-Ce) alkyl; each Ris is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, or -OH; each Rie is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloaikyi; or two Ri& together when attached to the same carbon can form -C=(0) when R& is
-(Co-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-Cs) spirocycloaikyi optionally substituted with one or more R23 when Re is
-(C0-C3) alkylene-heterocycloal kyl , -O-heterocycloalkyl, or
-N(Rs)-(Co-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (Cs-Cs) spiroheterocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl; or two R10 together when on adjacent atoms form a heterocycloaikyi ring optionally substituted with one or more R23; or two Rie together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more R23; or two R10 together with the atoms to which they are attached can form a bridged heterocycloaikyi ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or -N(Rs)-(Co-C3) alkylene-heterocycloalkyl,
each Ri7 is independently at each occurrence (Ci-C6) alkyl, (Ci-C6) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, cycloalkyl, heterocycloaikyi, or
-C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy and -OH; each Rig is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-C6) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-Ce) alkyl; each Ri9 and R20 is independently H, (Ci-Ce) alkyl, (C3-C8) cycloalkyl, -CH2C(0)NH2, -S(0)2(Ci-C6) alkyl, -S(0)2(C6-Cio) aryl or -C(0)(Ci-Ce) alkyl,
each R21 and R22 is independently H or (Ci-Ce) alkyl; each R2.3 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, or halogen, or two R23 together when attached to the same carbon form -C=(0); m is 1 or 2; and n is 0, 1, 2, or 3.
[0015] Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP28. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0016] Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP25. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0017] Another aspect of the disclosure relates to a method of treating a disease or disorder associated with inhibition of USP28 and USP25. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with inhibition of USP28 an effective amount of a compound of Formula (I), or a phannaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0018] Another aspect of the disclosure is directed to a method of inhibiting USP28. The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0019] Another aspect of the disclosure is directed to a method of inhibiting USP25. The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0020] Another aspect of the disclosure is directed to a method of inhibiting USP28 and USP25. The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof. [0021] Another aspect of the disclosure relates to a method of treating cancer. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0022] Another aspect of the disclosure relates to a method of treating inflammation. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0023] Another aspect of the disclosure relates to a method of treating an autoimmune disease. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0024] Another aspect of the disclosure relates to a method of treating an infectious disease. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
[0025] Another aspect of the disclosure relates to a method of treating a viral infection. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0026] Another aspect of the disclosure relates to a method of treating a bacterial infection. The method comprises administering to a patient in need thereof an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0027] Another aspect of the disclosure is directed to pharmaceutical compositions compri sing a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof and a pharmaceutically acceptable carrier. The pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant. [0028] Another aspect of the present disclosure relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in the manufacture of a medicament for treating a disease associated with inhibiting USP28.
[0029] Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP28.
[0030] Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP25.
[0031] Another aspect of the present disclosure relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the treatment of a disease associated with inhibiting USP28 and USP25.
[0032] The present disclosure further provides methods of treating a disease or disorder associated with modulation of USP28 and/or USP25 including, cancer, inflammation, an autoimmune disease, a viral infection, and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[0033] The present disclosure provides inhibitors of USP28 and/or USP25 that are therapeutic agents in the treatment of diseases, such as cancer, inflammation, autoimmune diseases, viral infections, and bacterial infections. Ultimately the present disclosure provides the medical community with a novel pharmacological strategy for the treatment of diseases and disorders associated with USP28 and/or USP25 enzymes.
Detailed Description of the Disclosure
[0034] The present disclosure relates to compounds and compositions that are capable of inhibiting the activity USP28 and/or USP25. The disclosure features methods of treating, preventing or ameliorating a disease or disorder in which USP28 and/or USP25 plays a role by administering to a patient in need thereof a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof. The methods of the present disclosure can be used in the treatment of a variety of USP28 and/or USP25 dependent diseases and disorders by inhibiting the activity of USP28 and/or USP25 enzymes. Inhibition of USP28 and/or USP25 provides a novel approach to the treatment, prevention, or amelioration of diseases including, but not limited to, cancer,
[0035] In a first aspect of the disclosure, the compounds of Formula (Γ) are described:
Figure imgf000012_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof, wherein Ri, R2, Rj, R4, R5, Rs1, Re, R7, X, m, and n are as described herein above.
[0036] The details of the disclosure are set forth in the accompanying description below. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, illustrative methods and materials are now described. Other features, objects, and advantages of the disclosure will be apparent from the description and from the claims. In the specification and the appended claims, the singular forms also include the plural unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. All patents and publications cited in this specification are incorporated herein by reference in their entireties.
Definitions
j0037] The articles "a" and "an" are used in this disclosure to refer to one or more than one (e.g., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element,
[0038] The term "and/or" is used in this disclosure to mean either "and" or "or" unless indicated otherwise. [0039] The term "optionally substituted" is understood to mean that a given chemical moiety (e.g., an alkyl group) can (but is not required to) be bonded other substituents (e.g., heteroatoms). For instance, an alkyl group that is optionally substituted can be a fully saturated alkyl chain (e.g., a pure hydrocarbon). Alternatively, the same optionally substituted alkyl group can have substituents different from hydrogen. For instance, it can, at any point along the chain be bounded to a halogen atom, a hydroxyl group, or any other substituent described herein. Thus the term "optionally substituted" means that a given chemical moiety has the potential to contain other functional groups, but does not necessarily have any further functional groups. Suitable substituents used in the optional substitution of the described groups include, without limitation, halogen, oxo, -OH, -CN, -COOH, -CH2CN, -O-(Ci-Ce) alkyl, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci- Ce) haloalkyl, (Ci-Ce) haioalkoxy, -0-(C2-C6) alkenyl, -0-(C2-C6) aikynyl, (C2-C6) alkenyl, (C2- Ce) ai kynyl, -OFF -OP(0)(OH)2, -OC(0)(Ci-C6) al kyl , -C(0)(Ci-Ce) alkyl, -ΟΠΟΧ Χ -Ο,) alkyl, - H2, - H((Ci-C6) alkyl), -N((Ci-Ce) alkyl)2, !( '(()}(( ! ·('.-,} alkyl, -C(0)NH(Ci-Ce) al kyl , -S(0)2(Ci-C6) alkyl, -S(0)NH(Ci-C6) alkyl, and S(0)N((Ci-C6) alkyl)2. The substituents can themselves be optionally substituted. "Optionally substituted" as used herein also refers to substituted or unsubstituted whose meaning is described below.
[0040] As used herein, the term "substituted" means that the specified group or moiety bears one or more suitable substituents wherein the substituents may connect to the specified group or moiety at one or more positions. For example, an aryl substituted with a cycloalkyi may indicate that the cycloalkyi connects to one atom of the aryl with a bond or by fusing with the aryl and sharing two or more common atoms.
[0041] As used herein, the term "unsubstituted" means that the specified group bears no substituents.
[0042] Unless otherwise specifically defined, the term "aryl" refers to cyclic, aromatic hydrocarbon groups that have 1 to 3 aromatic rings, including monocyclic or bicyclic groups such as phenyl, biphenyl or naphthyl. Where containing two aromatic rings (bicyclic, etc.), the aromatic rings of the aryl group may be joined at a single point (e.g., biphenyl), or fused (e.g., naphthyl). The aryl group may be optionally substituted by one or more substituents, e.g., 1 to 5 substituents, at any point of attachment. Exemplary substituents include, but are not limited to, -H, -halogen, -O-(Ci-Ce) alkyl, (Ci-Ce) alkyl, -0-(C2-Ce) alkenyl, -0-(C2-Ce) aikynyl, (C2-C6) alkenyl, (C2-C6) alkynyl, -OH, -OP(0)(QH)2, -OC(0)(C i-C6) alkyl, -C(0)(Ci-C6) alkyl, -OC(0)0(Ci-Ce) alkyl, -NH2, NH((Ci-C6) alkyl), N((Ci-C6) alkyl)2, -S(0)2-(Ci-C6) alkyl, -S(0)NH(Ci-Cfi) alkyl, and -S(0)N((C1-Ce) alkyl)2. The substituents can themselves be optionally substituted. Furthermore when containing two fused rings the aryl groups herein defined may have an unsaturated or partially saturated ring fused with a fully saturated ring. Exemplary ring systems of these aryl groups include, but are not limited to, phenyl, biphenyi, naphthyl, anthracenyl, phenalenyl, phenanthrenyl, indanyl, indenyl, tetrahydronaphthalenyl, tetrahydrobenzoannulenyi, and the like.
[0043] Unless otherwise specifically defined, "heteroaryl" means a monovalent monocyclic aromatic radical of 5 to 24 ring atoms or a polycyclic aromatic radical, containing one or more ring heteroatoms selected from N, O, and S, the remaining ring atoms being C. Heteroaryl as herein defined also means a bicyclic heteroaromatic group wherein the heteroatom is selected from N, O, and S. The aromatic radical is optionally substituted independently with one or more substituents described herein. Examples include, but are not limited to, fury] , thienyl , pyrrolyl, pyridyl, pyrazolyl, pyrimidinyl, imidazolyl, isoxazolyl, oxazolyi, oxadiazolyl, pyrazinyl, indolyl, thiophen-2-yl, quinolyl, benzopyranyl, isothiazolyl, thiazolyl, thiadiazole, indazole, benzimidazolyl, thieno[3,2-b]thiophene, triazolyl, triazinyl, imidazo[l ,2-b]pyrazolyl, furo[2,3- cjpyridinyl, imidazo[l,2-a]pyridinyl, indazolyl, pyrrolo[2,3-c]pyridinyl, pyrrolo[3,2-c]pyridinyl, pyrazolo[3,4-c]pyridinyl, thieno[3,2-c]pyridinyi, thieno[2,3-c]pyridinyl, thieno[2,3-b]pyridinyl, benzothiazolyl, indolyl, indolinyi, indolinonyl, dihydrobenzothiophenyi, dihydrobenzofuranvl, benzofuran, chromanyl, thiochromanyl , tetrahydroquinolinyl, dihydrobenzothiazine, dihydrobenzoxanyi, quinolinyl, isoquinolinyi, 1,6-naphthyridinyl, benzo[de]isoquinolinyi, pyrido[4,3-b][l ,6]naphthyridinyl, thieno[2,3-b]pyrazinyl, quinazolinyl, tetrazolo[l ,5-a]pyridinyl, [l,2,4]triazolo[4,3-a]pyridinyl, isoindolyl, pyrrolo[2,3-b]pyridinyl, pyrrolo[3,4-b]pyridinyl, pyrrolo[3,2-b]pyridinyl, imidazo[5,4-b]pyridinyl, pyrrolo[l,2-a]pyrimidinyl, tetrahydro pyrrolo[l ,2-a]pyrimidinyl, 3,4-dihydro-2H-l 2-pyrrolo[2, l-b]pyrimidine, dibenzo[b,d] thiophene, pyridin-2-one, furo[3,2-c]pyridinyl, furo[2,3-c]pyridinyl, l H-pyrido[3,4-b][l,4] thiazinyl, benzooxazolyl, benzoisoxazolyl, furo[2,3-b]pyridinyl, benzothiophenyl, 1,5- naphthyridinyl, furo[3,2-b]pyridine, [l,2,4]triazolo[l.,5-a]pyridinyl, benzo [l,2,3]triazolyl, imidazo[ 1 ,2-a]pyrimidinyl, [ 1 ,2,4]triazoio[4,3 -bjjpyridazinyl, benzo[c] [ 1 ,2,5]thiadiazolyi, benzo[c][l,2,5]oxadiazole, l ,3-dihydro-2H-benzo[d]imidazol-2-one, 3,4-di hydro-2H-pyrazolo [l,5-b][l,2]oxazinyl, 4,5,6,7-tetrahydropyrazolo[l ,5-a]pyridinyl, thiazolo[5,4-d]thiazolyl, imidazo[2, l-b][l,3,4]thiadiazolyl, thieno[2,3-b]pyrrolyl, 3H-indolyl, and derivatives thereof. Furthermore when containing two fused rings the heteroaryl groups herein defined may have an unsaturated or partially saturated ring fused with a fully saturated ring. Exemplary ring systems of these heteroaryl groups include indoliny] , indolinonyl, dihydrobenzothiophenyl, dihydrobenzofuran, chromanyl, thiochromanyl, tetrahydroquinolinyi, dihydrobenzothiazine, 3,4- dihydro-lH~ isoquinolinyl, 2,3 -dihydrobenzofuran, indolinyl, indolyl, and dihydrobenzoxanyl.
[0044] Halogen or "halo" refers to fluorine, chlorine, bromine, or iodine.
[0045] Alkyl refers to a straight or branched chain saturated hydrocarbon containing 1-12 carbon atoms. Examples of a (Ci-Ce) alkyl group include, but are not limited to, methyl, ethyl, propyl, butyl, pentyi, hexyl, isopropyl, isobutyi , sec-butyl, fert-butyl, isopentyl , neopentyl, and isohexyi.
[0046] "Alkoxy" refers to a straight or branched chain saturated hydrocarbon containing 1- 12 carbon atoms containing a terminal "O" in the chain, e.g., -O(alkyl). Examples of alkoxy groups include without limitation, methoxy, ethoxy, propoxy, butoxy, t-butoxy, or pentoxy groups.
[0047] The term "aikyiene" or " alkyl enyl" refers to a divalent alkyl radical . Any of the above mentioned monovalent alkyl groups may be an aikyiene by abstraction of a second hydrogen atom from the alkyl . As herein defined, alkvlene may also be a Co-Ce alkvlene. An alkvlene may further be a Co-C4 aikyiene. Typical aikyiene groups include, but are not limited to, -CH2-, - CH(CH3)-, -Π Π ! = } --, -CH2CH2-, -CH2CH(CH3)-, -Π 1 :C(C i 1 ψ~, -CH2CH2CH2-,
-CH2CH2CH2CH2-, and the like.
[0048] "Cycloalkyl" or "carbocyclyl" means monocyclic or polycyclic saturated carbon rings containing 3-18 carbon atoms. Examples of cycloalkyl groups include, without limitations, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptanyi, cyclooctanyl, norboranyi, norborenyl, bicyclo[2.2.2]octanyl, or bicyclo[2,2.2]octenyl and derivatives thereof. A C:<-Cg cycloalkyl is a cycloalkyl group containing between 3 and 8 carbon atoms. A cycloalkyl group can be fused (e.g., decalin) or bridged (e.g., norbomane). [0049] "Heterocyclyl" or "heterocycloalkyl" monocyclic or polycyclic rings containing carbon and heteroatoms taken from oxygen, nitrogen, or sulfur and wherein there is not delocalized π electrons (aromaticity) shared among the ring carbon or heteroatoms. The heterocycloalkyl ring structure may be substituted by one or more substituents. The substituents can themselves be optionally substituted. Examples of heterocyclyl rings include, but are not limited to, oxetanyl, azetadinyl, tetrahydrofuraiiyl, tetrahydropyraiiyl, pyrroiidiiiyl, oxazolinyl, oxazolidinyi, thiazolinyi, thiazolidinyl, pyranyl, thiopyranyl, tetrahydropyranyl, dioxalinyi, piperidinyl, morpholinyl, thiomorpholinyl, thiomorpholinyl S-oxide, thiomorpholinyl S-dioxide, piperazinyl, azepinyl, oxepinyl, diazepinyl, tropanyl, oxazolidinonyl, and homotropanyl.
[0050] The term "hydroxyalkyl" means an alkyl group as defined above, where the aikyi group is substituted with one or more OH groups. Examples of hydroxyalkyl groups include HO- CH2~, HO-CH2-CH2- and Cft-CHfQH)-.
[0051] The term "haloalkyl" as used herein refers to an alkyl group, as defined herein, which is substituted one or more halogen. Examples of haloalkyl groups include, but are not limited to, trifluoromethyi, difluoromethyl, pentafiuoroethyi, trichloromethyi, etc.
[0052] The term "haloalkoxy" as used herein refers to an alkoxy group, as defined herein, which is substituted one or more halogen. Examples of haloalkyl groups include, but are not limited to, trifluoromethoxy, difluoromethoxy, pentafluoroethoxy, trichloromethoxy, etc.
[0053] The term "cyano" as used herein means a substituent having a carbon atom joined to a nitrogen atom by a triple bond, e.g., C≡N.
[0054] "Spirocycloalkyl" or "spirocyclyl" means carbogenic bicyclic ring systems with both rings connected through a single atom. The ring can be different in size and nature, or identical in size and nature. Examples include spiropentane, spriohexane, spiroheptane, spirooctane, spirononane, or spirodecane. One or both of the rings in a spirocycle can be fused to another ring carbocyclic, heterocyclic, aromatic, or heteroaromatic ring. One or more of the carbon atoms in the spirocycle can be substituted with a heteroatom (e.g., O, N, S, or P). A (C3-C12) spirocy cloalkyl is a spirocycle containing between 3 and 12 carbon atoms. One or more of the carbon atoms can be substituted with a heteroatom. [0055] The term "spiroheterocycloalkyl" or "spiroheterocyclyl" is understood to mean a spirocycle wherein at least one of the rings is a heterocycle (e.g., at least one of the rings is furanyl, morpholinyl, or piperadinyl).
[0056] The term "solvate" refers to a complex of variable stoichiometry formed by a solute and solvent. Such solvents for the purpose of the disclosure may not interfere with the biological activity of the solute. Examples of suitable solvents include, but are not limited to, water, MeOH, EtOH, and AcOH. Solvates wherein water is the solvent molecule are typically referred to as hydrates. Hydrates include compositions containing stoichiometric amounts of water, as well as compositions containing variable amounts of water.
[0057] The term "isomer" refers to compounds that have the same composition and molecular weight but differ in physical and/or chemical properties. The structural difference may be in constitution (geometric isomers) or in the ability to rotate the plane of polarized light (stereoisomers). With regard to stereoisomers, the compounds of Formula (I) may have one or more asymmetric carbon atom and may occur as racemates, racemic mixtures and as individual enantiomers or diastereomers.
[0058] The disclosure also includes pharmaceutical compositions comprising an effective amount of a disclosed compound and a pharmaceutically acceptable carrier. Representative "pharmaceutically acceptable salts" include, e.g., water-soluble and water-insoluble salts, such as the acetate, amsonate (4,4-diaminostilbene-2,2-disulfonate), benzenesulfonate, benzonate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium, calcium edetate, camsylate, carbonate, chloride, citrate, clavulariate, dihydrochloride, edetate, edisylate, estolate, esylate, fumerate, fiunarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexafluorophosphate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, magnesium, malate, maieate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, 3-hydroxy-2-naphthoate, oieate, oxalate, paimitate, pamoate (1, 1-methene-bis- 2-hydroxy-3-naphthoate, einbonate), pantothenate, phosphate/diphosphate, picrate, polygalacturonate, propionate, p-toluenesulfonate, salicylate, stearate, subacetate, succinate, sulfate, sulfosalicylate, surarnate, tannate, tartrate, teoclate, tosvlate, triethiodide, and valerate salts. [0059] A "patient" or "subject" is a mammal, e.g., a human, mouse, rat, guinea pig, dog, cat, horse, cow, pig, or non-human primate, such as a monkey, chimpanzee, baboon or rhesus.
[0060] An "effective amount" when used in connection with a compound is an amount effective for treating or preventing a disease in a subject as described herein.
[0061] The term "carrier", as used in this disclosure, encompasses carriers, excipients, and diluents and means a material, composition or vehicle, such as a liquid or solid fil ler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting a pharmaceutical agent from one organ, or portion of the body, to another organ, or portion of the body of a subject.
[0062] The term "treating" with regard to a subject, refers to improving at least one symptom of the subject's disorder. Treating includes curing, improving, or at least partially ameliorating the disorder.
[0063] The term "disorder" is used in this disclosure to mean, and is used interchangeably with, the terms disease, condition, or illness, unless otherwi se indicated,
[0064] The term "administer", "administering", or "administration" as used in this disclosure refers to either directly administering a disclosed compound or pharmaceutically acceptable salt of the disclosed compound or a composition to a subject, or administering a prodrug derivative or analog of the compound or pharmaceutically acceptable salt of the compound or composition to the subject, which can form an equivalent amount of active compound within the subject's body.
[0065] The term "prodrug," as used in this disclosure, means a compound which is converti ble in vivo by metabolic means (e.g., by hydrolysis) to a disclosed compound.
[0066] The term "cancer" includes, but is not limited to, the following cancers: bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g. : squamous cell carcinoma), colorectal cancer (e.g., adenocarcinoma), esophageal cancer (e.g., squamous cell carcinoma), gastric cancer (e.g. : adenocarcinoma, medulloblastoma, colon cancer, choriocarcinoma, squamous cell carcinoma), head and neck cancer, hematologic cancer (e.g., acute lymphocytic anemia, acute myeloid leukemia, acute lymphoblastic B cell leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt' s lymphoma, chronic lymphocytic leukemia, chronic eosinophillic leukemia/hypereosinophillic syndrome, chronic myeloid leukemia, Hodgkin' s lymphoma, mantle ceil lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchi oloalveolar adenocarcinoma, mesothelioma, mucoepidermoid carcinoma, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma, squamous cell carcinoma), liver cancer (e.g., hepatocellular carcinoma), lymphoma, neurological cancer (e.g., glioblastoma, neuroblastoma, neuroglioma), ovarian (e.g., adenocarcinoma), pancreatic cancer (e.g., ductal carcinoma), prostate cancer (e.g., adenocarcinoma), renal cancer (e.g., renal cell carcinoma, clear cell renal carcinoma), sarcoma (e.g., chondrosarcoma, E wings sarcoma, fibrosarcoma, multipotential sarcoma, osteosarcoma, rhabdomyosarcoma, synovial sarcoma), skin cancer (e.g. melanoma, epidermoid carcinoma, squamous cell carcinoma), thyroid cancer (e.g., medullary carcinoma), and uterine cancer.
[0067] As used herein, the terms "autoimmune di sease" or "autoimmune disorder" refer to a condition that is immune-mediated due to an attack on self-tissues, such as when a subject's own antibodies react with host tissue, but can also involve an immune response to a microorganism . Examples of autoimmune diseases include, but are not limited to, multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto's disease, Guillain-Barre' syndrome, Grave's disease, myasthenia gravis, Sjogren's syndrome, nodular panarteritis, autoimmune enteropathy, uveitis, autoimmune oophoritis, chronic immune thrombocytopenic purpura, colitis, diabetes, psoriasis, pemphigus vulgaris, proliferative glomerulonephritis, Wiskott-Aldrich syndrome, autoimmune lymph oproliferative syndrome, chronic arthritis, inflammatory chronic rhinosinusitis, colitis, celiac disease, inflammatory bowel disease, Barrett's esophagus, i nil animator)' gastritis, autoimmune nephritis, autoimmune vasculitis, autoimmune hepatitis, autoimmune carditis, autoimmune encephalitis, and autoimmune mediated hematological disease.
[0068] The present disclosure relates to compounds or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, capable of inhibiting USP28 and/or USP25, which are useful for the treatment of diseases and disorders associated with modulation of a USP28 and/or USP25 enzyme. The disclosure further relates to compounds, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, which are useful for inhibiting USP28 and/or USP25.
[0069] In any of the embodiments of the invention, the cancer can be any cancer in any organ, for example, a cancer is selected from the group consisting of glioma, thyroid carcinoma, breast carcinoma, small-cell lung carcinoma, non-small-cell carcinoma, gastric carcinoma, colon carcinoma, gastrointestinal stromal carcinoma, pancreatic carcinoma, bile duct carcinoma, CNS carcinoma, ovarian carcinoma, endometrial carcinoma, prostate carcinoma, renal carcinoma, anaplastic large-cell lymphoma, leukemia, multiple myeloma, mesothelioma, and melanoma, and combinations thereof.
[0070] The present invention relates to compounds or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, capable of inhibiting USP28 and/or USP25, which are useful for the treatment of diseases and disorders associated with modulation of a USP28 and/or USP25 enzyme. The invention further relates to compounds, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, which are useful for inhibiting USP28 and/or USP25.
[0071] In one embodiment, the compounds of Formula (I) have the structure of Formula (la):
Figure imgf000020_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof, wherein:
X is N or CR-;;
Ri is I f , (C !-(';>) alkyl, (C1-C0) alkoxy, (Ci-Gs) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloaikyi, -CN, or -NRARKI; R2 is H, (C1-C&) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloaikyi, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or -NRnR12;
R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloaikyi, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or -NR13R1 ;
wherein at least one of Ri, R2, or R3 is not H; or Ri and R2 together form a (C i~C \; cycloalkyl optionally substituted with one or more
Ris; or R2 and R3 together form a (C4-Cs) cycloalkyl optionally substituted with one or more
Rl5,
R4 is H, (Ci-Ce) alkyl, or (Ci-Ce) haloaikyi;
R? is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloaikyi;
Rs' is H, (Ci-Ce) alkyl, halogen, or (C i ·('.-.} haloaikyi;
Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
-O-heterocycloalkyl, -(C0-C3) alkylene-aryl, -(C0-C3) alkylene-heteroaryl or
-N(R8)-(C0-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl, aryl and heteroaryi are optionally substituted with one or more Rie;
each R? is independently at each occurrence H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloaikyi, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, (Cs-Cs) cycloalkyl, heterocycloalkyl, aryl, or heteroaryi, wherein the alkyl is optionally substituted with one or more (Ci-Ce) alkoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryi are optionally substituted with one or more Ri?; or
Re and R;< together when on adjacent atoms form a (Ci-Cs) cycloalkyl ring optionally substituted with one or more Ris; or Re and R7 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Rig; Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one or more Rig; or Re and R? together when on adjacent atoms form a heteroaryi ring optionally substituted with one or more Ris; or two R? together when on adjacent atoms form a (tVCg) cycloalkyl ring; or two R? together when on adjacent atoms form a heterocycloalkyl ring; two R? together when on adjacent atoms form an aryl ring; or two R? together when on adjacent atoms form a heteroaryi ring;
Figure imgf000022_0001
each R9, Rio, R11, R12, R13, and R14 is independently H, (Ci-Ce) al kyl , or
-C(0)(Ci-C6) alkyl; each Ri5 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, o -OH; each Ri6 is independently at each occurrence (C i -( V.) alkyl, (d-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -C(0) R2iR22, - S(0} '(C !-C,-.} alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl , or two R16 together when attached to the same carbon can form -C=(0) when R6 is
-(Co-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R )-(Co-C3) al kylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-C8) spirocycloaikyi optionally substituted with one or more R23 when Re is
-(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloal kyl, or
-N(R8)-(C0-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-Cs) spiroheterocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl, or two Rie together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more R2.3; or two Rie together when on adjacent atoms form a heteroaryi ring optionally substituted with one or more R23; or two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or -N(R8)-(Co-C3) alkylene-heterocycloalkyl;
each Ri7 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, cycloalkyl, heterocycloalkyl, or
-C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy and -OH; each His is independently at each occurrence (C i -( V.) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, -C(Q)OH, or -C(0)0(Ci-Ce) alkyl; each Ri9 and R20 is independently H, (Ci-Ce) alkyl, (CB-CS) cycloalkyl, -CH2C(0)NH2, •S{()) H( : ·( ',.} alkyl, -S(0)2(Ce-Cio) aryl or C(0){(V( V) alkyl; each R?j and R22 is independently H or (Ci-Ce) alkyl; each R23 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce.) haloalkyl, (Ci-Ce) haloalkoxy, or halogen; or two R23 together when attached to the same carbon form -C=(0); and n is 0, 1, 2, or 3.
0072 In another embodiment, the compounds of Formula (I) have the structure of Formula
Figure imgf000023_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0073] In another embodiment, the compounds of Formula (I) have the structure of Formula (Ic):
Figure imgf000023_0002
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0074] In another embodiment, the compounds of Formula (I) have the structure of Formula (Id):
Figure imgf000024_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0075] In another embodiment, the compounds of Formula (I) have the structure of Formula (Ie):
Figure imgf000024_0002
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0076] In another embodiment, the compounds of Formula (I) have the structure of Formula (If):
Figure imgf000024_0003
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0077] In another embodiment, the compounds of Formula (I) have the structure of Formula (¾):
Figure imgf000025_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0078] In another embodiment, the compounds of Formula (I) have the structure of Formula (Hi):
Figure imgf000025_0002
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0079] In another embodiment, the compounds of Formula (I) have the stracture of Formula (li):
Figure imgf000025_0003
6 (Ii), and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0080] In another embodiment, the compounds of Formula (I) have the structure of Formula (¾):
Figure imgf000026_0001
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0081] In another embodiment, the compounds of Formula (I) have the structure of Formula (Ik):
Figure imgf000026_0002
and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, and tautomers thereof.
[0082] In some embodiments of the Formulae above,
R& is -(Co-Cs) alkylene-C(0)OH, -(C0-C3) alkylene-heteroeycloafkyf,
-(C0-C3) alkylene-aryl, -(Co-C3) alkylene-heteroaryl or -N(R8)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more Ri6, and
each Hie. is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hvdroxyalkyl, halogen, (C3-Cs) cycioalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl; or two Ri6 together when attached to the same carbon can form -C=(0) when e is
-(Co-C3) alkylene-heterocycloalkyl or -N(R8)-(C0-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-Cs) spirocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, or -N(R8)-(Co-C3) alkylene- heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-Cs) spiroheterocycloaikyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene- heterocycloalkyl, or -N(R8)-(Co-C3) alkylene-heterocycloalkyl ; or two Rie together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more R23; or two Rie together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more R23; or two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene- heterocycloalkyl or -N(Rs)-(C0-C3) alkylene-heterocycloalkyl .
[0083] In some embodiments of the Formulae above, X is CR7. In another embodiment, X is
N.
[0084] In some embodiments of the Formulae above, Ri is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or -NR9R10, In another embodiment, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloal kyl , (Ci-C4) haloalkoxy, (Ci-C4) hydroxyalkyl, halogen, (C3-Ce) cycloalkyl, -CN, or -NR9R10. In yet another embodiment, Ri is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl,
(Ci-Cs) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, (C3-Ce) cycloalkyl, -CN, or -NR9R10. In another embodiment, Ri is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl,
(C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, (Cs-Ce) cycloalkyl, or -NR9R10. In yet another embodiment, Ri is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, -CN, or -NR9R10. In another embodiment, Ri is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (Cs-Ce) cycloalkyl, or -NR9R10. In yet another embodiment, Ri is H or (C1-C4) alkyl . In another embodiment, Ri is (Ci-C4) alkyl. [0085] In some embodiments of the Formulae above, R?. is H, (C i -( ' i) alkyl,
(d-C4) alkoxy, (C1-C4) haloalkyl, (Ci-d) haloalkoxy, (Ci-C4) hydroxvalkyl, halogen,
(Cs-Ce) cycloalkvl, or -NR11R12. In another embodiment, R2 is H, (C1-C3) alkyl ,
(C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen,
(C3-C6) cycloalkyl, or -NR11R12. In yet another embodiment, R2 is H, (C1-C4) alkyl,
(Ci-C4) haloalkyl, halogen, {( ' ;-( '.,} cycloalkyl, or -NRnR12. In another embodiment, R2 is H, (C1-C3) alkyl, (C1-C3) haloalkyl, halogen, (tVCe) cycloalkyl, or -NR11R12. In yet another embodiment, R2 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (C3-C0) cycloalkyl, or -NRiiRi?, In another embodiment, R2 is H or halogen.
[0086] In another embodiment, Ri and R2 together form a (C4-C») cycloalkyl optionally substituted with one to three R15 , In yet another embodiment, Ri and R2 together form a
(Gi-Ce) cycloalkyl optionally substituted with one to three Ris.
[0087] In some embodiments of the Formulae above, R3 is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (Ci-d) haloalkoxy, (Ci-Ci) hydroxyalkyl, halogen, (CVCe) cycloalkyl, or - R13R14. In another embodiment, R3 is H, (€- -€3) alkyl, (O-C3) alkoxy, (C1-C3) haloalkyl, (O-C3) haloalkoxy,
Figure imgf000028_0001
hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR13R14. In yet another embodiment, R3 is H, (C1-C4) alkyl, (C1-C4) alkoxy, or (C1-C4) haloalkyl. In another embodiment, R3 i s H, (O-C3) alkyl, (C1-C3) alkoxy, or (C1-C3) haloalkyl. In another embodiment, R3 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (Cs-Ce) cycloalkyl, or -NRORM. In another embodiment, R3 is H.
[0088] In some embodiments of the Formulae above, at least one of Ri, R2, or R3 is not H.
[0089] In some embodiments of the Formulae above, Ri is (Ci-Cc) alkyl, (Ci-Ce) alkoxy, (C1-C0) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NR R10; and R?. is H, (C1-C&) alkyl, (C1-C&) alkoxy, (Ct-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-C8) cycloalkyl, or -NRuR12.
[0090] In some embodiments of the Formulae above, Ri i s II, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or - R9R10; and R2 is (Ci-Ce) alkyl, (Ci-Cc) alkoxy, (Ci-Ce) haloalkyl, (Ci-Cc) haloalkoxy, (Ci- Ce) hydroxyalkyl, halogen, (C3-C8) cycloalkyl, or -NRnR12.
[0091] In some embodiments of the Formulae above, Ri is (C 1-C4) alkyl, (d-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10; and R2 is I f . (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, (Cs-Ce) cycloalkyl, or -NR11R12; and R3 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (Ci-C3) hydroxyalkyl, halogen,
(C3-C6) cycloalkyl, 01- -NR13R14.
[0092] In some embodiments of the Formulae above, Ri is H, (Ci-Ce) alkyl, (C1-C0) alkoxy, (C1-C0) haloalkyl, (Ci-Gs) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NR9R10; R2 is (Ci-Cb) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cs) cycloalkyl, or -NR11R12; and R3 is H, (Ci-Ce) alkyl, (Ci-C6) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cs) cycloalkyl, or -NRnRi4.
[0093] In some embodiments of the Formulae above, Rj is (C1-C4) alkyl, (Ci-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10; R2 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (iVO) haloalkyl, (C1-C3) haloalkoxy,
(C1-C3) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR11R12, and R3 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C 1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen,
(C3-C6) cycloalkyl, or -NR RM, and R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cg) cycloalkyl, or -NRBRM.
[0094] In some embodiments of the Formulae above, Ri is H, (Ci-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkyl, or -NR9R10; R2 is H, (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy,
(C1-C3) hydroxyalkyl, halogen, (Cs-Gs) cycloalkyl, or -NRnR12; and Rj is (Ci~C3) alkyl,
(O-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen,
(CVCe) cycloalkyl, or -NRuRu. and R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce.) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or -NRDRM.
[0095] In another embodiment, R2 and R3 together form a (C4-Cs) cycloalkyl optionally substituted with one to three R15. In yet another embodiment, R2 and R3 together form a (C4-C0) cycloalkyl optionally substituted with one to three R15.
[0096] In some embodiments of the Formulae above, R4 is H, (C1-C3) alkyl, or
(d-C3) haloalkyl. In another embodiment, R¾ is H or (C1-C3) alkyl. In yet another embodiment, R4 is H. In another embodiment, R4 is H, methyl, ethyl, n-propyl, or iso-propyl. In yet another embodiment, R4 is H or methyl.
[0097] In some embodiments of the Formulae above, R5 is H, (C1-C3) alkyl, halogen, or (Ci-C3) haloalkyl. In another embodiment, R5 is H, halogen, or (C1-C3) alkyl. In yet another embodiment, R5 is H or (Ci-C3) alkyl. In another embodiment, R5 is H. In yet another embodiment, Rs is H, F, CI, methyl, ethyl, n-propyl, or iso-propyl. In another embodiment, Rs is H, methyl, ethyl, n-propyl, or iso-propyl. In yet another embodiment, Rs is H, F, or methyl. In another embodiment, R5 is H or methyl.
[0098] In some embodiments of the Formulae above, R.v is H, (C1-C3) alkyl, halogen, or (Ci~C3) haloalkyl. In another embodiment, Rs' is H, halogen, or (C1-C3) alkyl. In yet another embodiment, R.v is H or (C1-C3) alkyl. In another embodiment, R.v is H. In yet another embodiment, Rs' is H, F, CI, methyl, ethyl, n-propyl, or iso-propyl. In another embodiment, Rs' is H, methyl, ethyl, n-propyl, or iso-propyl. In yet another embodiment, Rs' is H, F, or methyl. In another embodiment, Rs1 is H or methyl.
[0099] In some embodiments of the Formulae above, R¾ and R5 together with the carbon atoms to which they are attached form a (C3-Cs) cycloalkyl ring. In another embodiment, R and Rs together with the carbon atoms to which they are attached form a (C4-Ce) cycloalkyl ring. In another embodiment, m is 2 and R4 and R5 together with the carbon atoms to which they are attached form a (C4-Ce) cycloalkyl ring.
[00100] In some embodiments of the Formulae above, Re is -(Co~C?) alkyl ene-C(0)OH,
-(C0-C2) alkylene-heterocycloalkyl, -O-heterocycloalkyl, -(C0-C2) alkylene-aryl,
-(C0-C2) alkylene-heteroaryl or -N(R8)-(C0-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyi, aryl and heteroaryl are optionally substituted with one or more Ri6. In another embodiment, Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
-(Co-C3) alkylene-aryl, -(Co-C3) alkylene-heteroaryl or -N(R8)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyi, aryl and heteroaryl are optionally substituted with one to three R10.
In another embodiment, Re is -(Co-C3) alkylene-C(0)OH, -(Co-C3) alkylene-heterocycloalkyl, -(C0-C3) alkylene-heteroaryl or -N(Rs)-(C0-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl and heteroaryl are optionally substituted with one to three R16. In another embodiment, Re is -(C0-C3) alkylene-heterocycloalkyl, -(C0-C3) alkylene-heteroaryl or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl and heteroaryl are optionally substituted with one to three Rie. In another embodiment, Re is
-(C0-C3) alkylene-heterocycloalkyl, -O- heterocycloalkyl, -(C0-C3) alkylene-heteroaryl or -N(R8)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl and heteroaryl are optionally substituted with one to three Rie. In another embodiment, e is -O- heterocycloalkyl optionally substituted with one to three R10.
[00101] In some embodiments of the Formulae above, R? is H, (Ci-C4) alkyl, (Ci-C4) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, halogen, -OH, -CN, (C -C8) cycloalkvl, heterocycloalkyl, aryl, or heteroaryl, wherein the alkyl is optionally substituted with one or more (Ci-Gs) aikoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one to three R17. In another embodiment, R? is H, (C1-C4) alkyl, (C 1-C4) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, halogen, -OH, -CN, (C3-Cs) cycloalkyl, aryl, or heteroaryl, wherein the alkyl is optionally substituted with one or more (C1-C4) aikoxy or -OH, and wherein the cycloalkyl, aryl, and heteroaryl are optionally substituted with one to three R17.
[00102] In another embodiment, Re and R? together when on adjacent atoms form a
(C3-C8) cycloalkyl ring optionally substituted with one to three Ris. In yet another embodiment. Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one to three R18. In another embodiment, Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one to three R18. In yet another embodiment, Re and R? together when on adjacent atoms form a heteroaryl ring optionally substituted with one to three Rie.
[00103] In another embodiment, two R? together when on adjacent atoms form a
(C3-C8) cycloalkyl ring. In yet another embodiment, two R? together when on adjacent atoms form a heterocycloalkyl ring. In another embodiment, two R? together when on adjacent atoms form an aryl ring. In yet another embodiment, two R? together when on adjacent atoms form a heteroaryl ring. I] In some embodiments of the Formulae above, Rs is H or (C1-C3) alkyl. In another embodiment, R.8 is H. In yet another embodiment, Rs is (C1-C3) alkyl. In another embodiment, Rg is H, methyl, ethyl, n-propyl, or iso-propyl. In another embodiment Rs is H, methyl, or ethyl .
[00105] In some embodiments of the Formulae above, R is H, (C1-C3) alkyl, or
-C(0)(C1-C3) alkyl . In another embodiment, R9 is H, (Ci-C.3) alkyl, or -C(0)(Ci~C2) alkyl. In yet another embodiment, Rs is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
In some embodiments of the Formulae above, Rio is H, (C1-C3) alkyl, or
-C(0)(C1-C3) alkyl. In another embodiment, R10 is H, (C1-C3) alkyl, or -C(0)(C1-C2) alkyl. In yet another embodiment, Rio is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
Figure imgf000032_0001
[00107] In some embodiments of the Formulae above, R11 is H, (C1-C3) alkyl, or
('((>)(( C alkyl. In another embodiment, R11 is H, (C1-C3) alkyl, or -C(0)(Ci-C2) alkyl. In yet another embodiment, R11 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
-C(0)CH2CH3. In another embodiment, Rn is H.
[00108] In some embodiments of the Formulae above, R12 is H, (C1-C3) alkyl, or
-C(0)(Ci-C3) alkyl. In another embodiment, R12 is H, (C1-C3) alkyl, or { '(()){{ ' !-(" ··) alkyl. In yet another embodiment, R12 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
-C(0)CFi2CH3. In another embodiment, R12 is H.
In some embodiments of the Formulae above, R13 is H, (C1-C3) alkyl, or
-C(0)(C1-C3) alkyl . In another embodiment, R13 is H, (C1-C3) alkyl, or -C(0)(Ci~C2) alkyl. In yet another embodiment, R13 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
-C(0)CH2CH3. In yet another embodiment, R13 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or -C(0)CH2CH3. In another embodiment, R13 is H,
[00110] In some embodiments of the Formulae above, R14 is H, (C1-C3) alkyl, or
-C(0)(Ci-C3) alkyl . In another embodiment, R14 is H, (C1-C3) alkyl, or -C(0)(Ci-C2) alkyl. In yet another embodiment, R14 is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or
-C(0)CH2CH3. In yet another embodiment, RM is H, methyl, ethyl, n-propyl, iso-propyl, -C(0)CH3, or -C(0)CH2CH3. In another embodiment, R14 is H, j 1 [00111] In some embodiments of the Formulae above, Ris is (C1-C3) aikyi, (C1-C3) alkoxy, (d-C3) haloalkyl, (C1-C3) haloalkoxy, halogen, or -OH. In another embodiment, R15 is
(C1-C3) alkyl, (C1-C3) haloalkyl, halogen, or -OH. In yet another embodiment, R15 is
(Ci-Cs) alkyl, halogen, or -OH. In another embodiment, R15 is (C1-C3) aikyi or -OH. In yet another embodiment, R15 is -OH.
[00112] In some embodiments of the Formulae above, R10 is (Ci-CV) alkyl, (Ci-C4) alkoxy, (Ci-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxy alkyl, halogen, (C3-C8) cycloalkyl, -C(0)"NR21R22, -S(0)2(Ci-C6) al kyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-C6) alkoxy, OH, and heterocvcloalkyl. In another embodiment, Ri6 is (C1-C4) alkyl, (C1-C4) alkoxy,
(C1-C4) hydroxyalkyl, halogen, (Cs-Cg) cycloalkyl, -C(0)NR_iR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl. In yet another embodiment, R10 is (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) hydroxyalkyl, halogen,
(C3-C8) cycloalkyl, or - R19R20, wherein the al kyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl. In another embodiment, Rie is (Ci-Gs) alkyl, (C3-Cs) cycloalkyl, -C(0)NR2iR22, ~S(0)2(Ci-Ce) alkyl, -OH, or - R19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloal kyl . In yet another embodiment, Rie is (C1-C4) aikyi, (C3-Cs) cycloalkyl, or -NR19R20, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl.
[00113] In another embodiment, two Rie together when attached to the same carbon can form -C=::(0) when Re is -(Co-C3) alkylene-heterocycloalkyl or -N(R8)-(Co-C3) alkylene- heterocycloalkyl. In yet another embodiment, two Rie together when attached to the same atom form a (C3-C8) spirocycloalkyl optionally substituted with one to three R23 when Re is
-(C0-C3) alkylene-heterocycloalkyl or -N(R8)-(Co-C3) aikyiene-heterocycloaikyi. In another embodiment, two Rie together when attached to the same atom form a
(C3-C8) spiroheterocycloaikyi optionally substituted with one to three R23 when Re is -(C0-C3) alkylene-heterocycloalkyl or -N(R8)-(C0-C3) alkylene-heterocycloalkyl. In another embodiment, two Ri6 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one to three R23. In another embodiment, two Rie together when on adjacent atoms form a heteroaryi ring optionally substituted with one to three R23. In another embodiment, two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) aikyiene-heterocycloaikyi or - N(R8)-(Co-C3) alkylene-heterocycloalkyl ,
[00114] In another embodiment, two Rie together when attached to the same carbon can form C (()) when R6 is -(C0-C3) alkylene-heterocycloalkyl, -O -heterocycloalkyl, or
-N(R8)-(Co-C3) aikyiene-heterocycloaikyi. In another embodiment, two R16 together when attached to the same atom form a (Cs-Cg) spirocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -Q-heteroeycioalkyl, or
-N(Rs)-(C0-C3) aikyiene-heterocycloaikyi. In another embodiment, two Rie together when attached to the same atom form a (C3-Cs) spiroheterocycloal kyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycioalkyl, or
-N(Rs)-(C0-C3) alkylene-heterocycloalkyl. In another embodiment, two Rie together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more R23. In another embodiment, two Rie together when on adjacent atoms form a heteroaryi ring optionally substituted with one or more R2.3. In another embodiment, two Rie together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(Rs)-(C0-C3) alkylene-heterocycloalkyl .
[00115] In some embodiments of the Formulae above, R17 i s (Ci-Ci) alkyl, (C1-C4) aikoxy, (Ci-C4) haloalkvl, (C1-C4) haloaikoxy, halogen, cycioalkyl, heterocycloalkyl, or
~C(0)~heterocycloalkyl, wherein the alkyl is optionally substituted with one to three substituents independently selected from (C1-C4) aikoxy and -OH. In another embodiment, Rn is
(0-C4) alkyl, (Ci-d) haloalkyl, halogen, heterocycloalkyl, or -C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one to three substituents independently selected from (Ci-Oj) aikoxy and -OH.
[00116] In some embodiments of the Formulae above, Ris is (C1-C4) alkyl, (C1-C4) aikoxy, (C1-C4) haloalkyl, (C1-C4) haloaikoxy, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In another embodiment, Ri8 is (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In yet another embodiment, Ris is (C1-C4) alkyl, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In another embodiment, Ris is (C1-C4) alkyl, -OH, -C(0)OH, or -C(0)0(Ci-C4) alkyl. In another embodiment, Ris is -C(0)OH, or
-C(0)0(Ci-C4) alkyl.
[00117] In some embodiments of the Formulae above, R1 is H, (C1-C4) alkyl,
(C3-C8) cycloalkyl, -CFl2C(0)NH2, -S(0)2(Ci-C4) alkyl, -S(0)2(C6-Cio) aryl or
-C(0)(Ci-C4) alkyl. In another embodiment, R19 is H, (C1-C4) alkyl, (C'i-Ce) cycloalkyl, -CH2C(0) H2, -S(0)2(Ci-C4) alkyl, -S(0)2(C6-Cio) aryl, or -C(0)(Ci-C4) alkyl. In yet another embodiment, R19 is H, (Ci-Cs) alkyl or (C3-Ce) cycloal kyl . In another embodiment, R19 i s H, (Ci-C4) alkyl -CH2C(0)NH2, -S(0)2(Ci-C4) alkyl, -S(0)2(C6-Cio) aryl, or -C(0)(Ci-C4) alkyl.
[ 18] In some embodiments of the Formulae above, R20 is H, (C1-C4) alkyl,
(Cs-Ce) cycloal kyl, -Ci K"i()}\ ! ! <, -S(0)2(Ci-C4) al kyl , -S(0)2(C6-Cio) aryl or
~C(0)(Ci-C4) alkyl. In another embodiment, Ria ls H, (C1-C4) alkyl, (Cs-Ce) cycloalkyl, -CH2C(0)Ni-l2, -S(0)2(Ci-C4) al kyl , -S(0)2(C6-Cio) aryl, or -C(0)(Ci-C4) alkyl. In yet another embodiment, R20 is H, (C1-C4) alkyl or (( ' ;·('.-.} cycloalkyl. In another embodiment, R20 is H, (C1-C4) alkyl -CI W(0)\ l . -S(0)2(Ci-C4) alkyl, -S(0)2(Ce-Cio) aryl, or -C(0)(Ci-C4) alkyl.
[00119] In some embodiments of the Formulae above, R21 i s H or (C1-C3) alkyl. In another embodiment, Rn is H, methyl, ethyl, n-propyi, or iso-propyl.
In some embodiments of the Formulae above, R22 is H or (C1-C3) alkyl . In another embodiment, R22 is H, methyl, ethyl, n-propyi, or iso-propyl .
L21] In some embodiments of the Formulae above, R23 is (C1-C3) alkyl, (Ci-Cs) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxv, or halogen. In another embodiment, R23 is (C1-C2) alkyl, (Ci-C2) alkoxy, (Ci-C2) haloalkyl, (Ci-C2) haloalkoxv, or halogen. In yet another embodiment, R23 is (C1-C2) alkyl, (C1-C2) haloalkyl, or halogen. In another embodiment, R23 is (Ci-C2) alkyl, or (C1-C2) haloalkyl. In another embodiment, R23 is (C1-C3) alkyl. In another embodiment, R23 is methyl, ethyl, n-propyl, or iso-propyl.
[00122] In another embodiment, two R23 together when attached to the same carbon form -C=(0).
[00123] In some embodiments of the Formulae above, n is 0, 1, or 2. In another embodiment, n is 0 or I . In vet another embodiment, n is 1 , 2. or 3. In another embodiment, n is J or 2. In another embodiment, n is 2 or 3. In another embodiment, n is 0. In another embodiment, n is 1. In another embodiment, n is 2. In another embodiment, n is 3.
[00124] In some embodiments of the Formulae above, X is CH.
[00125] In some embodiments of the Formulae above, R4 i s H or CH3 and R? is H or CH3.
[00126] In some embodiments of the Formulae above, 4 is H or CH3, Rs is H or CH3, and Rs is H, ffuoro, or CH3.
[00127] In some embodiments of the Formulae above, R2 is H, (Ci-Ce) alkyl,
(Ci-C6) haloalkyl, halogen, (C3-C8) cycloalkvl, or -NH2.
[00128] In some embodiments of the Formulae above, Ri and R2 together form a
(C3-C8) cycloalkvl optionally substituted with one or more Ri.
[00129] In some embodiments of the Formulae above, R3 i s H, (Ci-Ce.) alkyl, (Ci-Ce) alkoxy, or (Ci-Ce) haloalkyl.
[00130] In some embodiments of the Formulae above, R2 and R3 together form a
(C3-C8) cycioalkyl optionally substituted with one or more R15.
[00131] In another embodiment, Ri is H, (Ci-C4) alkyl, (Ci-C4) alkoxy, (Ci-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C6) cycloalkvl, or - 9R10; R2 is (C1-C3) alkyl, (C1-C3) alkoxy, (C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, (C3-C6) cycioalkyl, or ~NR.11R.12; and R3 is H, (C1-C3) alkyl, (C1-C3) alkoxy,
(C1-C3) haloalkyl, (C1-C3) haloalkoxy, (C1-C3) hydroxyalkyl, halogen, ((' *-( ',·, ) cycioalkyl, or
[00132] In some embodiments of the Formulae above, X is CR7. In another embodiment, X is CR7 and Ri is H, (C1-C4) alkyl, (C i-C i) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (C3-C8) cycioalkyl, or -NR9R10. In yet another embodiment, X is CR7, Ri is H, (C1-C4) alkyl , (C1-C4) al koxy, (Ci-Gs) haloalkyl, (C1-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (C3-Cs) cycioalkyl, or --NR9R10, and R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycioalkyl, or -NR11R12. In another embodiment, X is CR7, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (C3-C8) cycioalkyl, or -NR9R10, R2 is H, (C1-C4) alkyl,
(C1-C4) haloalkyl, halogen, (C3-C8) cycioalkyl, or -NR11R12, and R3 is H, (C1-C0) alkyl, (Ci-Ce) alkoxy, (d-d) haloalkyl. In yet another embodiment, X is CR7, Ri is H, (d-d) alkyl, (Ci-d) alkoxy, (Ci-d) haloalkyl, (d-d-) haloalkoxy, (d-C4) hydroxyalkyl, halogen,
(d-Cs) cycloalkyi, 01- --NR9R10, Ri is H, (C1-C4) alkyl, (d-d) haloalkyl, halogen,
(d-d) cycloalkyi, or - R11R12, R3 is H, (d-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, and R4 is H or (d-d) alkyl . In another embodiment, X is CR7, Ri is H, (C1-C4) alkyl,
(Ci-C4) alkoxy, (Ci-C4) haloalkyl, (Ci-C4) haloalkoxy, (d-d) hydroxyalkyl, halogen,
(d-d) cycloalkyi, or -NR9R10, R2 is H, (d-d) alkyl, (C1-C4) haloalkyl, halogen,
(d-Cs) cycloalkyi, or -NR11R12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C1-C3) alkyl, and R5 is H or (d-d) alkyl. In yet another embodiment, X is CR7, Ri is H, (d-d) alkyl, (Ci-Ci) alkoxy, (d-d) haloalkyl, (d -d) haloalkoxy, (d-C4) hydroxyalkyl, halogen, (d-d) cycloalkyi, or -NR R10, R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyi, or -NR11R12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (Ci-Cs) alkyl, Rs is H or (C1-C3) alkyl, and Rs' is H or (Ci-d) alkyl.
[00133] In another embodiment, X is CR7, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-Cs) cycloalkyi, or -NR9R10, R2 is H, (C1-C4) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyi, or - R11R12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce.) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C1-C3) alkyl, R5 is H or (Ci- C3) alkyl, R.v is H or (C1-C3) alkyl, and e is -(C0-C3) alkylene-heterocycloalkyl optionally substituted with one or more ie.
[00134] In another embodiment, X is CR7, Ri i s H, (C1-C4) alkyl, (Ci-Ci) alkoxy,
(Ci-C4) haloalkyl, (C1-C4) haloalkoxy, (Ci-d) hydroxyalkyl, halogen, (C3-Cs) cycloalkyi, or -NR9R10, R2 is H, (C1-C4) al kyl , (C1-C4) haloal kyl , halogen, (Cs-Cs) cycloalkyi, or -NRnR12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C1-C3) alkyl, Rs is H or (Ci- C3) alkyl, Rs' is H or (C1-C3) alkyl, and Re is -O-heterocycloalkyl optionally substituted with one or more Rie.
[00135] In another embodiment, X is CR7, Ri is H, (d-d) alkyl, (Ci-d) alkoxy,
(C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (Cj-Cs) cycloalkyi, or -NR9R10, R2 is H, (Ci-d) alkyl, (C1-C4) haloalkyl, halogen, (d-d) cycloalkyi, or -NRiiRi?., R3 is H, (Ci-Ce) alkyl, (C1-C0) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (d-d) alkyl, Rs is I I or (C1-C3) alkyl, Rs- is H or (d-d) alkyl, and Re is -(C0-C3) alkylene-C(0)OH. [00136] In another embodiment, X is CR?, Ri is H, (d-d) alkyl, (d-d) alkoxy, (d-d) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, R.2 is H, (d-d) al ky] , (C1-C4) haloal kyl , halogen, (d-d) cycloalkyl, or -NRiiRi2, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (d -d) haloalkyl, R4 is H or (Ci-d) alkyl, Rs is H or (C1-C3) alkyl, R_v i s H or (d-d) alkyl, and Re is -(C0-C3) alkylene-heteroaryl optionally substituted with one or more Rie.
[00137] In another embodiment, X is CR7, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(d-d) haloalkyl, (d-d) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (d-d) cycloalkyl, or -NR11R12, R3 is H, (d-d) alkyl, (d-d) alkoxy, (d-d) haloalkyl, Rs is H or (d-d) alkyl, R5 is H or (d-d) alkyl, R.v is H or (d-d) alkyl, and Re is -N(Rs)-(Co-d) alkylene-heterocycloaikyi optionally substituted with one or more Rie.
[00138] In another embodiment, X is CR7, Ri is H, (d-d) alkyl, (d-d) alkoxy,
(d-d) haloalkyl, (d-d) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-d) cycloalkyl, or - R9R10, R2 is H, (C1-C4) alkyl, (C i-d ) haloalkyl, halogen, (C3-Cs) cycloalkyl, or -NR11R12, R3 is H, (Ci-Ce) alkyl, (d-d) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C1-C3) alkyl, R5 is H or (Ci- C3) alkyl, Rs1 is H or (d-d) alkyl, and Re is -(Co-d) alkylene-aryl optionally substituted with one or more Rie.
[00139] In another embodiment, X is CR7, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(d-d) haloalkyl, (d-d) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, R?. is H, (C1-C4) alkyl, (Ci-d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or --NR11R12, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (d-d) alkyl, R5 is H or (C1-C3) alkyl, y is H or (C1-C3) alkyl, and Re and R7 together when on adjacent atoms form a (d-d) cycloalkyl ring optionally substituted with one or more Rig.
[00140] In another embodiment, X is CR7, Ri is H, (d-d) alkyl, (d-d) alkoxy,
(d-d) haloalkyl, (d-d) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, R2 is H, (d-d) alkyl, (d-d) haloalkyl, halogen, (d-d) cycloalkyl, or -NRnR12, R3 is H, (Ci-Ce) alkyl, (d-d) alkoxy, (Ci-Ce) haloalkyl, R4 i s H or (d-d) alkyl, R5 is H or (C1-C3) alkyl, Rs' is H or (d-d?) alkyl, and Re and R7 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris [00141] In some embodiments of the Formulae above, X is N. In another embodiment, X is N and Ri is H, (d-d) alkyl, (C1-C4) alkoxy, (d-d) haloalkyl, (Ci-d) haloalkoxy,
(d-d) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or -NR9R10. In yet another embodiment, X is
N, Ri is H, (d-d) alkyl, (CJ -C4) alkoxy, (C1-C4) haloalkyl, (Ci-C4) haloalkoxy,
(C1-C4) hydroxyalkyl, halogen, (d-d) cycloalkyl, or -NR9R10, and R2 is H, (C1-C4) alkyl,
(Ci-C4) haloalkyl, halogen, (d-Cs) cycloalkyl, or -NRHR12. In another embodiment, X is N, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or -NR9R10, II2 is H, (d-d) alkyl, (d-d) haloalkyl, halogen,
(d-Cs) cycloalkyl, or -NRnR12, and R3 is H, (d-d) alkyl, (Ci-d) alkoxy, (d-d) haloalkyl. In yet another embodiment, X is N, Ri is H, (d-d) alkyl, (d-d) alkoxy, (C1-C4) haloalkyl,
(Ci-C4) haloalkoxy, (Ci-C4) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or -NRgRio, R2 is H,
{( ' ) -{ ' i ) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyl, or -NR11R12, R3 is H,
(d-d) alkyl, (d-d) alkoxy, (Ci-Ce) haloalkyl, and R4 is H or (d-d) alkyl. In another embodiment, X is N, Ri is H, (d-d) alkyl, (C1-C4) alkoxy, (C1-C4) haloalkyl,
(d-d) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-C8) cycloalkyl, or -NR9R10, R2 is H,
(d-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyl, or -NRuR12, R3 is H,
(Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C 1-C3) alkyl, and Rs is H or
(C1-C3) alkyl. In yet another embodiment, X is N, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(d-C4) haloalkyl, (C1-C4) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or
-NR9R10, R2 is H, (d-d) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or - R11R12, R? is H, (Ci-Ce) alkyl, (d-d.) alkoxy, (Ci-Ce) haloalkyl, R4 i s H or (C1-C3) alkyl, R5 is H or
(Ci-C3) alkyl, and R5' is H or (d-d) alkyl.
[00142] In another embodiment, X is N, Ri is H, (d-d) alkyl, (d-d) alkoxy,
(d-d) haloalkyl, (d-d) haloalkoxy, (d-d) hydroxyalkyl, halogen, (d-Cs) cycloalkyl, or -NR9R10, R2 is H, (d-d) alkyl, (d -d) haloalkyl, halogen, (C3-Cs) cycloalkyl, or - R11R12, R? is H, (Ci-Ce) alkyl, (d-d) alkoxy, (d-d) haloalkyl, R4 i s H or (d-d) alkyl, R5 is H or (C 1-C3) alkyl, Rs1 is H or (d-d) alkyl, and Re is -(C0-C3) alkylene-heterocycioalkyl optionally substituted with one or more Ri6.
[00143] In another embodiment, X is N, Ri is H, (d-d) alkyl, (d-d) alkoxy,
(Ci-d) haloalkyl, (d-d) haloalkoxy, (Ci-d) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or -NR9R10, R?. is H, (C1-C4) alkyl, (d-d) haloalkyl, halogen, (d -d) cycloalkyi, or -NR11R12, R? is H, (d-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (C1-C3) alkyl, R5 is H or (d-d) al ky] , R5' is H or (C1-C3) al kyl , and Re is -O-heterocycloalkyl optionally substituted with
Figure imgf000040_0001
[00144] In another embodiment, X is N, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(d-C4) haloalkyl, (Ci-d) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, ((' ;-< \) cycloalkyi, or -NR9R10, R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyi, or - R11R12, R3 is H, (d-Ce) alkyl, (d-Ce) alkoxy, (d-Ce) haloalkyl, R4 i s H or (d-d) alkyl, R5 is H or (C1-C3) alkyl, Rs- is H or (C1-C3) alkyl, and Re is -(C0-C3) alkylene-C(0)OH.
[00145] In another embodiment, X is N, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(C1-C4) haloalkyl, (Ci-d) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (d-d) cycloalkyi, or -NR9R10, R2 is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (C3-C8) cycloalkyi, or - R11R12, R? is H, (d-Ce) alkyl, (d-Ce) alkoxy, (d-Ce) haloalkyl, R4 i s H or (d-d) alkyl, R5 is H or (d-d) alkyl, Rs- is H or (C1-C3) alkyl, and Re is -(C0-C3) alkyl ene-heteroaryl optionally substituted with one or more Rie.
[00146] In another embodiment, X is N, Ri is H, (d-d) alkyl, (C1-C4) alkoxy,
(Ci-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-Cs) cycloalkyi, or -NR9R10, R2 is H, (d-d) al kyl , (C1-C4) haloal kyl , halogen, (d-d) cycloalkyi, or -NRiiRi2, R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R4 is H or (Ci-Cs) alkyl, Rs is H or (C1-C3) alkyl, R5' is H or (C1-C3) alkyl, and Re is -N(Rs)-(Co-C3) alkylene-heterocycloalkyl optionally substituted with one or more Rie.
[00147] In another embodiment, X is N, Ri is H, (CJ-C4) alkyl, (Ci-C4) alkoxy,
(C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxyalkyl, halogen, (C3-Cg) cycloal kyi, or -NR9R10, R2 is H, (C i -C i ) alkyl, (C1-C4) haloalkyl, halogen, (Cs-Cg) cycloalkyi, or -NRiiRi?., R3 is It (d-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R¾ is H or (Ci-C3) alkyl, Rs is H or
-C3) alkyl, Ry is H or iCV(' ;} alkyl, and Re is -(C0-C3) alkylene-aryl optionally substituted with one or more Rie,
[00148] In another embodiment, X is N, Ri is H, (d-C4) alkyl, (d-C4) alkoxy,
(C1-C4) haloalkyl, (d-C4) haloalkoxy, (Ci-C4) hydroxyalkyl, halogen, (d-Cs) cycloalkyi, or -NR9R10, R2 is H, (C1-C4) alkyl, (Ci-C ) haloalkyl, halogen, (Cs-Cs) cycloalkyl, or ~NRnRi2, Rs is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (C1-C0) haloalkyl, R4 is H or (C1-C3) alkyl, R5 is H or (C1-C3) alkyl, Ry is H or (C1-C3) alkyl, and Re and R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring optionally substituted with one or more Ris.
[00149] In another embodiment, X is N, Ri is H, (C1-C4) alkyl, (C1-C4) alkoxy,
(C1-C4) haloalkyl, (C1-C4) haloalkoxy, (C1-C4) hydroxy alkyi, halogen, (C3-Cs) cycloalkyl, or -NR9R10, Ri is H, (C1-C4) alkyl, (C1-C4) haloalkyl, halogen, (Ci-Cs) cycloalkyl, or -NRnRi2, R? is H, (C1-C0) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, R is H or (C1-C3) alkyl, R5 is H or (C1-C3) alkyl, R5' is H or (C1-C3) alkyl, and Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris,
[00150] Non-limiting illustrative compounds of the disclosure include:
Figure imgf000041_0001
Figure imgf000041_0002
40
144628010 vl
Figure imgf000042_0001
Figure imgf000042_0002
Figure imgf000042_0003
Figure imgf000042_0004
Figure imgf000042_0005
41 628010 vl
Figure imgf000043_0001
Figure imgf000043_0002
Figure imgf000043_0003
Figure imgf000043_0004
Figure imgf000043_0005
Figure imgf000043_0006
010
Figure imgf000044_0001
4∞4ΚφάΒ|3.2. t fssia
Figure imgf000044_0002
Figure imgf000044_0003
Figure imgf000044_0004
Figure imgf000044_0005
Figure imgf000044_0006
Figure imgf000045_0001
Figure imgf000045_0002
Figure imgf000045_0003
Figure imgf000045_0004
Figure imgf000045_0005
44
144628010 vl
Figure imgf000046_0001
Figure imgf000046_0002
Figure imgf000046_0003
Figure imgf000046_0004
Figure imgf000046_0005
Figure imgf000046_0006
45 8010vl
Figure imgf000047_0001
Figure imgf000047_0002
«5. a-Si^-e- ^f ^ - j.S-lri ffi:
Figure imgf000047_0003
Figure imgf000047_0004
Figure imgf000047_0005
46
144628010 vi
Figure imgf000048_0001
Figure imgf000048_0002
Figure imgf000048_0003
Figure imgf000048_0004
Figure imgf000048_0005
Figure imgf000048_0006
4/ 628010 vl
Figure imgf000049_0001
Figure imgf000049_0002
Figure imgf000049_0003
Figure imgf000049_0004
Figure imgf000049_0005
Figure imgf000049_0006
628010 vl
Figure imgf000050_0001
-51. S-^TO- -|2,i^irai4-|^f3
Figure imgf000050_0002
Figure imgf000050_0003
Figure imgf000050_0004
Figure imgf000050_0005
49 28010 vl
Figure imgf000051_0001
Figure imgf000051_0002
Figure imgf000051_0003
Figure imgf000051_0004
Figure imgf000051_0005
504628010 vl
Figure imgf000052_0001
Figure imgf000052_0002
Figure imgf000052_0003
Figure imgf000052_0004
Figure imgf000052_0005
Figure imgf000052_0006
51
144628010 vl
Figure imgf000053_0001
Figure imgf000053_0002
Figure imgf000053_0003
Figure imgf000053_0004
Figure imgf000053_0005
Figure imgf000053_0006
Figure imgf000054_0001
Figure imgf000054_0002
Figure imgf000054_0003
Figure imgf000054_0004
Figure imgf000054_0005
Figure imgf000054_0006
53 8010 vl
Figure imgf000055_0001
Figure imgf000055_0002
Figure imgf000055_0003
Figure imgf000055_0004
Figure imgf000055_0005
54
Figure imgf000056_0001
Figure imgf000056_0002
Figure imgf000056_0003
Figure imgf000056_0004
Figure imgf000056_0005
144628010 vl
Figure imgf000057_0001
Figure imgf000057_0002
Figure imgf000057_0003
Figure imgf000057_0004
Figure imgf000057_0005
Figure imgf000058_0001
Figure imgf000058_0002
Figure imgf000058_0003
Figure imgf000058_0004
Figure imgf000058_0005
Figure imgf000058_0006
57
144628010 vl
Figure imgf000059_0001
Figure imgf000059_0002
Figure imgf000059_0003
MOB. NKP ~2^ - -d;i®^k^sfe
Figure imgf000059_0004
Figure imgf000059_0005
Figure imgf000059_0006
58
628010 vl
Figure imgf000060_0001
Figure imgf000060_0002
Figure imgf000060_0003
Figure imgf000060_0004
Figure imgf000060_0005
59
Figure imgf000061_0001
Figure imgf000061_0002
Figure imgf000061_0003
-113.3-¾*?S!Sfr^^
Figure imgf000061_0004
Figure imgf000061_0005
-;i . i^-smi^-^ ^N^H^si -122. , -≤^^^-1-ν«^-=ί;·ν
Figure imgf000061_0006
60
28010 )
Figure imgf000062_0001
Figure imgf000062_0002
M25. 4^,^ s≠^4. ^S ^
Figure imgf000062_0003
Figure imgf000062_0004
Figure imgf000062_0005
61628010 vl
Figure imgf000063_0001
Figure imgf000063_0002
Figure imgf000063_0003
Figure imgf000063_0004
Figure imgf000063_0005
Figure imgf000063_0006
28010 vl
Figure imgf000064_0001
2 , ^-li^ss^s-i^s^^s^side
Figure imgf000064_0002
* 2.
Figure imgf000064_0003
634628010vl
Figure imgf000065_0001
M . -1 2. S>*¾m½^a-s^¾^ -C3^^e
Figure imgf000065_0002
Figure imgf000065_0003
64
628010 vl
Figure imgf000066_0001
Figure imgf000066_0002
Figure imgf000066_0003
Figure imgf000066_0004
65 628010 vl
Figure imgf000067_0001
Figure imgf000067_0002
Figure imgf000067_0003
Figure imgf000067_0004
Figure imgf000068_0001
Figure imgf000068_0002
Figure imgf000068_0003
Figure imgf000068_0004
67 28010 vl
Figure imgf000069_0001
Figure imgf000069_0002
Figure imgf000069_0003
Figure imgf000069_0004
Figure imgf000069_0005
68 8010 vl
Figure imgf000070_0001
Figure imgf000070_0002
1-1" J4.2-(4-f2- «^^^s§ !fe∑¾sf
Figure imgf000070_0003
Figure imgf000070_0004
Figure imgf000070_0005
69
144628010vl
Figure imgf000071_0001
Figure imgf000071_0002
^ sss¾ sa
Figure imgf000071_0003
-179. !^mm^ ^ ^ i^x
Figure imgf000071_0004
Figure imgf000071_0005
Figure imgf000071_0006
70
628010 vl
Figure imgf000072_0001
Figure imgf000072_0002
Figure imgf000072_0003
Figure imgf000072_0004
Figure imgf000072_0005
71 8010 v!
Figure imgf000073_0001
Figure imgf000073_0002
Figure imgf000073_0003
Figure imgf000073_0004
Figure imgf000073_0005
Figure imgf000073_0006
2, 3-¾fe«¾d¾se-2«cs:rtesms e
144628010 vl
Figure imgf000074_0001
Figure imgf000074_0002
Figure imgf000074_0003
Figure imgf000074_0004
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73
144628010 'ϊ
Figure imgf000075_0001
Figure imgf000075_0002
Figure imgf000075_0003
Figure imgf000075_0004
Figure imgf000075_0005
74 28010 vl
Figure imgf000076_0001
Figure imgf000076_0002
Figure imgf000076_0003
Figure imgf000076_0004
Figure imgf000076_0005
Figure imgf000076_0006
75 010 vl
Figure imgf000077_0001
Figure imgf000077_0002
Figure imgf000077_0003
!-2m ^W-I -215. **πΛβι>Ηφ < κο* φ∞
Figure imgf000077_0004
Figure imgf000077_0005
76
628010 l
Figure imgf000078_0001
Figure imgf000078_0002
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Figure imgf000078_0005
Figure imgf000078_0006
and
144628010 vl [00151] In another embodiment of the disclosure, the compounds of Formula (I) are enantiomers. In some embodiments the compounds are the (^-enantiomer. In other embodiments the compounds are the (R)-enantiomer. In yet other embodiments, the compounds of Formula (I) may be (+) or (-) enantiomers.
[00152] It should be understood that all isomeric forms are included within the present disclosure, including mixtures thereof. If the compound contains a double bond, the substituent may be in the E or Z configuration. If the compound contains a disubstituted cycloalkyl, the cycloalkyl substituent may have a cis- or trans configuration. All tautomeric forms are also intended to be included.
[00153] Compounds of the disclosure, and pharmaceutically acceptable salts, hydrates, solvates, stereoisomers and prodrugs thereof may exist in their tautomeric form (for example, as an amide or imino ether). All such tautomeric forms are contemplated herein as part of the present disclosure.
[00154] The compounds of the disclosure may contain asymmetric or chirai centers, and, therefore, exist in different stereoisomenc forms. It is intended that all stereoisomers forms of the compounds of the disclosure as well as mixtures thereof, including racemic mixtures, form part of the present disclosure. In addition, the present disclosure embraces all geometric and positional isomers. For example, if a compound of the disclosure incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the disclosure. Each compound herein disclosed includes all the enantiomers that conform to the general structure of the compound. The compounds may be in a racemic or enantiomerically pure form, or any other form in terms of stereochemistry. The assay results may reflect the data collected for the racemic form, the enantiomerically pure form, or any other form in terms of stereochemistry.
[00155] Diastereomeiic mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and/or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chirai auxiliary such as a chirai alcohol or Mosher's acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the
78
144628010 vl individual diastereomers to the corresponding pure enantioniers. Also, some of the compounds of the disclosure may be atropisomers (e.g., substituted biaryls) and are considered as part of this disclosure. Enantioniers can also be separated by use of a chiral HPLC column.
[00156] It is also possible that the compounds of the disclosure may exist in different tautomeric forms, and ail such forms are embraced within the scope of the disclosure. Also, for example, all keto-enol and imine-enamine forms of the compounds are included in the disclosure.
[00157] All stereoisomers (for example, geometric isomers, optical isomers and the like) of the present compounds (including those of the salts, solvates, esters and prodrugs of the compounds as well as the salts, solvates and esters of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this disclosure, as are positional isomers (such as, for example, 4-pyridyl and 3-pyridyl). (For example, if a compound of Formula (reincorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the disclosure. Also, for example, all keto-enol and imine- enamine forms of the compounds are included in the disclosure.) Individual stereoisomers of the compounds of the disclosure may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present disclosure can have the S or R configuration as defined by the RJPAC 1974 Recommendations. The use of the terms "salt", "solvate", "ester," "prodrug" and the like, is intended to equally apply to the salt, solvate, ester and prodrug of enantioniers, stereoisomers, rotamers, tautomers, positional isomers, racemates or prodrugs of the inventive compounds.
[00158] The compounds of Formula I may form salts which are also within the scope of this disclosure. Reference to a compound of the Formula herein is understood to include reference to salts thereof, unless otherwise indicated.
[00159] The present disclosure relates to compounds which are modulators of USP28 and/or USP25. In one embodiment, the compounds of the present disclosure are inhibitors of USP28 and/or USP25.
79
144628010 vl [00160] The disclosure is directed to compounds as described herein and pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof, and pharmaceutical compositions comprising one or more compounds as described herein, or pharmaceutically acceptable salts, hydrates, solvates, prodrugs, stereoisomers, or tautomers thereof.
Method of Synthesizing the Compounds
[00161] The compounds of the present disclosure may be made by a variety of methods, including standard chemistry. Suitable synthetic routes are depicted in the Schemes given below.
[00162] The compounds of Formula (I) may be prepared by methods known in the art of organic synthesis as set forth in part by the following synthetic schemes. In the schemes described below, it is well understood that protecting groups for sensitive or reactive groups are employed where necessary in accordance with general principles or chemistry. Protecting groups are manipulated according to standard methods of organic synthesis (T. W. Greene and P. G. M. Wuts, "Protective Groups in Organic Synthesis", Third edition, Wiley, New York 1999). These groups are removed at a convenient stage of the compound synthesis using methods that are readily apparent to those skilled in the art. The selection processes, as well as the reaction conditions and order of their execution, shall be consistent with the preparation of compounds of Formula (I).
[00163] Those skilled in the art will recognize if a stereocenter exists in the compounds of Formula (I). Accordingly, the present disclosure includes both possible stereoisomers (unless specified in the synthesis) and includes not only racemic compounds but the individual enantiomers and/or diastereomers as well. When a compound is desired as a single enantiomer or diastereomer, it may be obtained by stereospecific synthesis or by resolution of the final product or any convenient intermediate. Resolution of the final product, an intermediate, or a starting material may be affected by any suitable method known in the art. See. for example, "Stereochemistry of Organic Compounds" by E. L, Eliei, S. H. Wilen, and L. . Mander (Wiley- lnterscience, 1994),
[00164] The compounds described herein may be made from commercially available starting materials or synthesized using known organic, inorganic, and/or enzymatic processes.
80
144628010 vl Preparation of compounds
[00165] The compounds of the present invention can be prepared in a number of ways well known to those skilled in the art of organic synthesis. By way of example, compounds of the present invention can be synthesized using the methods described below, together with synthetic methods known in the art of synthetic organic chemistry, or variations thereof as appreciated by those skilled in the art. Preferred methods include but are not limited to those methods described below. Compounds of the present invention can be synthesized by following the steps outlined in General Schemes 1 and 2 which comprise different sequences of assembling intermediates 2a, 2b, 2c, 2d, and It. Starting materials are either commercially available or made by known procedures in the reported literature or as illustrated.
General Scheme 1
Figure imgf000082_0001
wherein R1-R5, R51, Re, Rr, m, and n are defined as in Formula (I).
[00166] The general manner of preparing target compounds of Formula (I) by using intermediates 2a and 2b, is outlined above in General Scheme 1. Coupling of carboxylic acid 2a with amine 2b under standard amide forming conditions using a coupling agent, e.g.. l-Ethyl-3- (3 -dimethyl aminopropy 1 jcarbodiimide with 1 -hydroxybenzotri azole (EDC I/HOB t), (Benzotriazol-l-yloxy)tris(dimethylamino)phosphonium hexafiuorophosphate (BOP), O- benzotriazole-N,'N,'N;N'-tetrarnethyl-uronium-hexaf!.uoro-phosphate (HBTU) or [bis (dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-b]pyridinium-3-oxide hexafiuorophosphate (HATU), and a base, e.g., triethylamine (TEA). Ν,Ν-diisopropylethylamine (DIEA), or 4- dimethylaminopyridine (DMAP), in a solvent {e.g. DCM or DMF, etc.) provides the desired product of Formula (I).
81
144628010 vl eneral Scheme 2
Figure imgf000083_0001
wherein R1-R5, Rs; Re, R?, m, and n are defined as in Formula (I).
[00167] Alternatively, compounds of Formula (I) can also be prepared by using intermediates 2b, 2c, 2d, and 2e as depicted in General Scheme 2 above. Acylation of 2b with 2c in the presence of a base, e.g.. triethylamine (TEA) or Ν,Ν-diisopropylethylamine (DIPEA), and in a solvent, e.g., DCM or THF, provides Intermediate 2d. Cyclization of 2d and 2e in the presence of a base, e.g., potassium carbonate (K2CO3) or sodium methoxide (NaOMe), and in a solvent, e.g., DMF or THF, optionally at elevated temperature provides the desired product of Formula (I).
[00168] Compounds of Formula (I) can exist as enantiomeric or diastereomeric stereoisomers. Enantionierically pure compounds of Formula (I) can be prepared using enantionierically pure chiral building blocks. Alternatively, racemic mixtures of the final compounds or a racemic mixture of an advanced intermediate can be subjected to chiral purification as described herein below to deliver the desired enantionierically pure intermediates or final compounds. In the instances where an advanced intermediate is purified into its individual enantiomers, each individual enantiomer can be carried on separately to deliver the final enantionierically pure compounds of Formula (I).
[00169] It should be understood that in the description and formula shown above, the various grou s Ri-Rs, Rs1, R6, R?, m, n, and other variables are as defined above, except where otherwise indicated, Furthermore, for synthetic purposes, the compounds of General Schemes 1 and 2 are
82
144628010 vl mere representative with elected radicals to illustrate the general synthetic methodology of the compounds of Formula (I) as defined herein.
Methods of Using the Disclosed Compounds
[00170] Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP28. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 an effective amount the compositions and compounds of Formula (I). In one embodiment, the disease or disorder is cancer.
[00171] In another aspect, the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 an effective amount the compositions and compounds of Formula (I). In one embodiment, the disease or disorder is cancer.
[00172] In another aspect, the present disclosure is directed to a method of inhibiting USP28. The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
[00173] Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP25. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP25 an effective amount the compositions and compounds of Formula (I). In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00174] In another aspect, the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP25 an effective amount the compositions and compounds of
83
144628010 vl Forrnu!a (I). In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00175] In another aspect the present disclosure is directed to a method of inhibiting USP25, The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
[00176] Another aspect of the disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with modulation of USP25. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 and USP25 an effective amount the compositions and compounds of Formula (I). In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00177] In another aspect, the present disclosure is directed to a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibition of USP28. The method comprises administering to a patient in need of a treatment for diseases or disorders associated with modulation of USP28 and USP25 an effective amount the compositions and compounds of Formula (I). In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00178] In another aspect, the present disclosure is directed to a method of inhibiting USP28 and USP25. The method involves administering to a patient in need thereof an effective amount of a compound of Formula (I).
[00179] Another aspect of the present di sclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of
84
144628010 vl USP28, the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I), in one embodiment, the disease or disorder is cancer,
[00180] Another aspect of the present disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of USP25, the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I), in one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00181] Another aspect of the present disclosure relates to a method of treating, preventing, inhibiting, or eliminating a disease or disorder in a patient associated with the inhibition of USP28 and USP25, the method comprising administering to a patient in need thereof an effective amount of a compound of Formula (I). In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00182] In another aspect, the present invention relates to a method of treating, preventing, inhibiting, or eliminating cancer. The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
[00183] In another aspect, the present invention relates to a method of treating, preventing, inhibiting, or eliminating inflammation. The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[00184] In another aspect, the present invention relates to a method of treating, preventing, inhibiting, or eliminating an autoimmune disease. The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof,
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144628010 vl [00185] in another aspect the present invention relates to a method of treating, preventing, inhibiting, or eliminating an infectious disease, The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof. In one embodiment, the infectious disease is a viral infection. In another embodiment, the infectious disease is a bacterial infection.
[00186] In another aspect, the present invention relates to a method of treating, preventing, inhibiting, or eliminating a viral infection. The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof,
[00187] In another aspect, the present invention relates to a method of treating, preventing, inhibiting, or eliminating a bacterial infection. The method comprises administering to a patient in need of a treatment for cancer an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof.
[00188] Another aspect of the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28. In one embodiment, the disease or disorder is cancer.
[00189] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP25. In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation, In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00190] Another aspect of the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method of treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28 and USP25. In one embodiment, the disease or disorder is cancer. In
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144628010 vl another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. Irs another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00191] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating cancer.
[00192] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating inflammation.
[00193] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating an autoimmune disease.
[00194] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating an infectious disease.
[00195] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating a viral infection,
[00196] In another aspect, the present invention relates to a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, for use in a method for treating, preventing, inhibiting, or eliminating a bacterial infection.
[00197] Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28. In one embodiment, the disease or disorder is cancer,
[00198] Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer
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144628010 vl thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP25, In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00199] Another aspect of the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or disorder associated with inhibiting USP28 and USP25. In one embodiment, the disease or disorder is cancer. In another embodiment, the disease or disorder is inflammation. In another embodiment, the disease or disorder is an autoimmune disease. In another embodiment, the disease or disorder is an infectious disease. In another embodiment, the disease or disorder is a viral infection. In another embodiment, the disease or disorder is a bacterial infection.
[00200] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating cancer.
[00201] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating inflammation,
[00202] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating autoimmune disorder.
[00203] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating an
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144628010 vl infection disease. In one embodiment, the infectious disease is a viral infection. In another embodiment, the infectious disease is a bacterial infection,
[00204] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a viral infection.
[00205] In another aspect, the present invention relates to the use of a compound of Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, or tautomer thereof, in the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a bacterial infection.
[00206] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with cancer.
[00207] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with inflammation,
[00208] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with an autoimmune disease.
[00209] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with an infectious disease, in one embodiment, the infectious disease is a viral infection. In another embodiment, the infectious disease is a bacterial infection.
[00210] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with a viral infection.
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144628010 vl [00211] In other embodiments, the present invention relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition or elimination of a disease or disorder associated with a bacterial infection.
[00212] The present disclosure also relates to the use of an inhibitor of USP28 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP28, wherein the medicament comprises a compound of Formula (I).
[00213] The present disclosure also relates to the use of an inhibitor of USP25 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP25, wherein the medicament comprises a compound of Formula (1),
[00214] The present disclosure also relates to the use of an inhibitor of USP28 and USP25 for the preparation of a medicament used in the treatment, prevention, inhibition, or elimination of a disease or condition mediated by USP28 and USP25, wherein the medicament comprises a compound of Formula (I).
[00215] In another aspect, the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP28, wherein the medicament comprises a compound of Formula (I),
[00216] Another aspect of the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP25, wherein the medicament comprises a compound of Formula (I).
[00217] In another aspect, the present disclosure relates to a method for the manufacture of a medicament for treating, preventing, inhibiting, or eliminating a disease or condition mediated by USP28 and USP25, wherein the medicament comprises a compound of Formula (i).
[00218] In some embodiments of the methods described herein, the cancer is selected from bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g., squamous cell carcinoma), colorectal cancer (e.g., adenocarcinoma), colon cancer, esophageal cancer (e.g., squamous cell carcinoma), gastric cancer (e.g., adenocarcinoma, choriocarcinoma, squamous cell carcinoma), head and neck cancer, hematologic cancer (e.g., acute lymphocytic anemia, acute
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144628010 vl myeloid leukemia, acute lymphoblastic B ceil leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt's lymphoma, chronic lymphocytic leukemia, chronic eosinophillic ieukemia/hypereosinophiliic syndrome, chronic myeloid leukemia, Hodgkin's lymphoma, mantle cell lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchi oloalveolar adenocarcinoma, mesothelioma, mucoepidermoid carcinoma, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma, squamous cell carcinoma), liver cancer (e.g., hepatocellular carcinoma), lymphoma, neurological cancer (e.g., glioblastoma, neuroblastoma, neuroglioma), ovarian cancer (e.g., adenocarcinoma), pancreatic cancer (e.g., ductal carcinoma), prostate cancer (e.g., adenocarcinoma), renal cancer (e.g., renal cell carcinoma, clear cell renal cancer carcinoma), sarcoma (e.g., chondrosarcoma, Ewings sarcoma, fibrosarcoma, multipotentiai sarcoma, osteosarcoma, rhabdomyosarcoma, synovial sarcoma), skin cancer (e.g., melanoma, epidermoid carcinoma, squamous cell carcinoma), thyroid cancer (e.g., medullar}' carcinoma), and uterine cancer. In some embodments, the cancer is a cancer that is sensitive to USP28 inhibition, in other embodments, the cancer is a cancer that is sensitive to USP25 inhibition. In other embodments, the cancer is a cancer that is sensitive to USP28 and USP25 inhibition.
[00219] In any of the embodiments of the disclosure, the cancer can be any cancer in any organ, for example, a cancer is selected from the group consisting of glioma, thyroid carcinoma, breast carcinoma, small-cell lung carcinoma, non-small-cell carcinoma, gastric carcinoma, colon carcinoma, gastrointestinal stromal carcinoma, pancreatic carcinoma, bile duct carcinoma, CNS carcinoma, ovarian carcinoma, endometrial carcinoma, prostate carcinoma, renal carcinoma, anaplastic large-cell lymphoma, leukemia, multiple myeloma, mesothelioma, and melanoma, and combinations thereof
[00220] In other embodiments, the cancer is selected from acute myeloid leukemia, gastric, pancreatic, colorectal, glioblastoma, neuroblastoma, small -ceil lung, non-small ceil lung, and squamous cell carcinoma.
[00221] In another embodiment, the present disclosure relates to a compound of Formula (I) or a pharmaceutical composition comprising a compound of the present disclosure and a pharmaceutically acceptable carrier used for the treatment of cancers including, but not limited to, bladder cancer, breast cancer (e.g., ductal carcinoma), cervical cancer (e.g., squamous cell
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144628010 vl carcinoma), colorectal cancer (e.g., adenocarcinoma), esophageal cancer (e.g., squamous cell carcinoma), gastric cancer (e.g., adenocarcinoma, choriocarcinoma, squamous cell carcinoma), head and neck cancer, hematologic cancer (e.g., acute lymphocytic anemia, acute myeloid leukemia, acute lymphoblastic B cell leukemia, anaplastic large cell lymphoma, B-cell lymphoma, Burkitt's lymphoma, chronic lymphocytic leukemia, chronic eosinophillic ieukemia/hypereosinophiliic syndrome, chronic myeloid leukemia, Hodgkin's lymphoma, mantle cell lymphoma, multiple myeloma, T-cell acute lymphoblastic leukemia), lung cancer (e.g., bronchioloalveolar adenocarcinoma, mesothelioma, mucoepidermoid carcinoma, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma, squamous cell carcinoma), liver cancer (e.g., hepatocellular carcinoma), lymphoma, neurological cancer (e.g., glioblastoma, neuroblastoma, neuroglioma), ovarian cancer (e.g., adenocarcinoma), pancreatic cancer (e.g., ductal carcinoma), prostate cancer (e.g., adenocarcinoma), renal cancer (e.g., renal cell carcinoma, clear cell renal cancer carcinoma), sarcoma (e.g., chondrosarcoma, Ewings sarcoma, fibrosarcoma, multi potential sarcoma, osteosarcoma, rhabdomyosarcoma, synovial sarcoma), skin cancer (e.g., melanoma, epidermoid carcinoma, squamous ceil carcinoma), thyroid cancer (e.g., medullar}' carcinoma), and uterine cancer. In other embodiments, the cancer is selected from acute myeloid leukemia, gastric cancer, pancreatic cancer, colorectal cancer, glioblastoma, neuroblastoma, small-cell lung cancer, non-small cell lung cancer, and squamous cell carcinoma,
[00222] In some embodiments, the patient is selected for treatment based on gene amplification and/or elevated tumor expression of USP28, MYC, LSD1, NICDl, and/or reduced expression of FBXW7 relative to tissue-matched expression.
[00223] In some embodiments, the patient is selected for treatment based on gene amplification and/or elevated tumor expression of USP28, USP25, MYC, LSD1, NICDl, and/or reduced expression of FBXW7 relative to tissue-matched expression.
[00224] In some embodiments, administration of a compound of Formula (I) or a pharmaceutical composition comprising a compound of the present disclosure and a pharmaceutically acceptable carrier induces a change in the cell cycle, ceil viability, cell apoptosis, or differentiation.
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144628010 vl [00225] For example, the change in the ceil cycle or cell viability or differentiation may be indicated by decreased tumor levels of MYC, LSD1, NICDl, PIM1, CDK1, POLA2, HEY1, and/or CCND1 , and/or increased levels of CD86 , p21, LGALS4, and/or DLL! .
[00226] In another embodiment, the present disclosure relates to a compound of Formula (I) or a pharmaceutical composition comprising a compound of the present disclosure and a pharmaceutically acceptable carrier used for the treatmeni of autoimmune diseases including, but not limited to, multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto's disease. Gui n-Barre' syndrome, Grave's disease, myasthenia gravis, Sjogren's syndrome, nodular panarteritis, autoimmune enteropathy, uveitis, autoimmune oophoritis, chronic immune thrombocytopenic purpura, colitis, diabetes, psoriasis, pemphigus vulgaris, proliferative glomerulonephritis, Wiskott-Aldrich syndrome, autoimmune lymphoproliferative syndrome, chronic arthritis, inflammatory chronic rhinosinusitis, colitis, celiac disease, inflammatory bowel disease, Barrett's esophagus, inflammatory gastritis, autoimmune nephritis, autoimmune vasculitis, autoimmune hepatitis, autoimmune carditis, autoimmune encephalitis, and autoimmune mediated hematological disease.
[00227] In any of the embodiments of the disclosure, the autoimmune disease can be, for example, an autoimmune disease selected from multiple sclerosis, psoriasis, intestine inflammatory disease, ulcerative colitis, Crohn's disease, rheumatoid arthritis and polyarthritis, local and systemic scleroderma, systemic lupus erythematosus, discoid lupus erythematosus, cutaneous lupus, cutaneous lupus erythematosus including chilblain lupus erythematosus, lupus nephritis, discoid lupus, subacute cutaneous lupus erythematosus, dermatomyositis, polymyositis, idiopathic myxedema, Hashimoto's disease, Guillain-Barre' syndrome, Grave's disease, myasthenia gravis, Sjogren's syndrome, nodular panarteritis, autoimmune enteropathy, uveitis, autoimmune oophoritis, chronic immune thrombocytopenic purpura, colitis, diabetes, psoriasis, pemphigus vulgaris, proliferative glomerulonephritis, Wiskott-Aldrich syndrome, autoimmune lymphoproliferative syndrome, chronic arthritis, inflammatory chronic rhinosinusitis, colitis, celiac disease, inflammatory bowel disease, Barrett's esophagus,
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144628010 vl inflammatory gastritis, autoimmune nephritis, autoimmune vasculitis, autoimmune hepatitis, autoimmune carditis, autoimmune encephalitis, and autoimmune mediated hematological disease.
[00228] Another aspect of the disclosure is directed to pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier. The pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.
[00229] In one embodiment, are provided methods of treating a disease or disorder associated with modulation of USP28 including cancer comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
[00230] In another embodiment, are provided methods of treating a disease or disorder associated with modulation of USP25 including cancer, inflammation, an autoimmune disease, a viral infection and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
[00231] In another embodiment, are provided methods of treating a disease or disorder associated with modulation of USP28 and USP25 including cancer, inflammation, an autoimmune disease, a viral infection and a bacterial infection, comprising administering to a patient suffering from at least one of said diseases or disorder a compound of Formula (I).
[00232] One therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 is to provide treatment to patients or subjects suffering from cancer.
[00233] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from cancer.
[00234] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from cancer.
[00235] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from inflammation.
[00236] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from inflammation.
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144628010 vl [00237] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from an autoimmune disease.
[00238] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from an autoimmune disease.
[00239] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from an infectious disease.
[00240] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from an infectious disease.
[00241] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from a viral infection.
[00242] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from a viral infection.
[00243] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP25 is to provide treatment to patients or subjects suffering from a bacterial infection.
[00244] Another therapeutic use of the compounds or compositions of the present disclosure which inhibit USP28 and USP25 is to provide treatment to patients or subjects suffering from a bacterial infection.
[00245] The disclosed compounds of the disclosure can be administered in effective amounts to treat or prevent a disorder and/or prevent the development thereof in subjects.
[00246] Administration of the disclosed compounds can be accomplished via any mode of administration for therapeutic agents, These modes include systemic or local administration such
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144628010 vl as oral, nasal, parenteral, transdermal, subcutaneous, vaginal, buccal, rectal or topical administration modes.
[00247] Depending on the intended mode of administration, the disclosed compositions can be in solid, semi-solid or liquid dosage form, such as, for example, injectables, tablets, suppositories, pills, time-release capsules, elixirs, tinctures, emulsions, syrups, powders, liquids, suspensions, or the like, sometimes in unit dosages and consistent with conventional pharmaceutical practices. Likewise, they can also be administered in intravenous (both bolus and infusion), intraperitoneal, subcutaneous or intramuscular form, and ail using forms well known to those skilled in the pharmaceutical arts.
[00248] Illustrative pharmaceutical compositions are tablets and gelatin capsules comprising a Compound of the Disclosure and a pharmaceutically acceptable carrier, such as a) a diluent, e.g., purified water, triglyceride oils, such as hydrogenated or partially hydrogenated vegetable oil, or mixtures thereof, corn oil, olive oil, sunflower oil, safflower oil, fish oils, such as EPA or DHA, or their esters or triglycerides or mixtures thereof, omega-3 fatty acids or derivatives thereof, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, sodium, saccharin, glucose and/or glycine; b) a lubricant, e.g., silica, talcum, stearic acid, its magnesium or calcium salt, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and/or polyethylene glycol; for tablets also; c) a binder, e.g., magnesium aluminum silicate, starch paste, gelatin, tragacantli, methylcellulose, sodium carboxymethyl cellulose, magnesium carbonate, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacantli or sodium alginate, waxes and/or polyvinylpyrrolidone, if desired, d) a disintegrant, e.g., starches, agar, methyl cellulose, bentonite, xanthan gum, algic acid or its sodium salt, or effervescent mixtures; e) absorbent, colorant, flavorant and sweetener; f) a emulsifier or dispersing agent, such as Tween 80, Labrasol, HPMC, DOSS, caproyl 909, iabrafac, labrafil, peceol, transcutol, capmul MCM, capmui PG-12, captex 355, gelucire, vitamin E TGPS or other acceptable emulsifier; and/or g) an agent that enhances absorption of the compound such as cyclodextrin, hydroxypropyl-cyclodextrin, PEG400, PEG200.
[00249] Liquid, particularly injectable, compositions can, for example, be prepared by dissolution, dispersion, etc. For example, the disclosed compound is dissolved in or mixed with a pharmaceutically acceptable solvent such as, for example, water, saline, aqueous dextrose,
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144628010 vl glycerol, ethanol, and the like, to thereby form an injectable isotonic solution or suspension. Proteins such as albumin, chylomicron particles, or serum proteins can be used to solubilize the disclosed compounds,
[00250] The disclosed compounds can be also formulated as a suppository that can be prepared from fatty emulsions or suspensions; using polyaikylene glycols such as propylene glycol, as the carrier.
[00251] The disclosed compounds can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, containing cholesterol, stearyl amine or phosphatidylcholines. In some embodiments, a film of lipid components is hydrated with an aqueous solution of drug to a form lipid layer encapsulating the drug, as described in U.S. Pat. No. 5,262,564 which is hereby incorporated by reference in its entirety.
[00252] Disclosed compounds can also be delivered by the use of monoclonal antibodies as individual carriers to which the disclosed compounds are coupled. The disclosed compounds can also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxyethylaspanamidephenol, or polyethyleneoxidepolylysine substituted with palmitoyl residues. Furthermore, the Disclosed compounds can be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polyiactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross-linked or amphipathic block copolymers of hydrogels. In one embodiment, disclosed compounds are not covalently bound to a polymer, e.g., a polycarboxylic acid polymer, or a polyacrylate.
[00253] Parental injectable administration is generally used for subcutaneous, intramuscular or intravenous injections and infusions. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions or solid forms suitable for dissolving in liquid prior to injection.
[00254] Another aspect of the disclosure is directed to pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier. The pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.
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144628010 vl [00255] Compositions can be prepared according to conventional mixing, granulating or coating methods, respectively, and the present pharmaceutical compositions can contain from about 0.1% to about 99%, from about 5% to about 90%, or from about 1% to about 20% of the disclosed compound by weight or volume.
[00256] The dosage regimen utilizing the disclosed compound is selected in accordance with a variety of factors including type, species, age, weight, sex and medical condition of the patient; the severity of the condition to be treated, the route of administration; the renal or hepatic function of the patient, and the particular disclosed compound employed. A physician or veterinarian of ordinary skill in the art can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.
[00257] Effective dosage amounts of the disclosed compounds, when used for the indicated effects, range from about 0.5 mg to about 5000 mg of the disclosed compound as needed to treat the condition. Compositions for in vivo or in vitro use can contain about 0,5, 5, 20, 50, 75, 100, 150, 250, 500, 750, 1000, 1250, 2500, 3500, or 5000 mg of the disclosed compound, or, in a range of from one amount to another amount in the list of doses. In one embodiment, the compositions are in the form of a tablet that can be scored.
Examples
[00258] The disclosure is further illustrated by the following examples and synthesis schemes, which are not to be construed as limiting this disclosure in scope or spirit to the specific procedures herein described. It is to be understood that the examples are provided to illustrate certain embodiments and that no limitation to the scope of the disclosure is intended thereby. It is to be further understood that resort may be had to various other embodiments, modifications, and equivalents thereof which may suggest themselves to those skilled in the art. without departing from the spirit of the present disclosure and/ or scope of the appended claims.
Analytical Methods, Materials, and Instrumentation
[00259] Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Proton nuclear magnetic resonance (NMR) spectra were obtained on either Bruker or Varian spectrometers at 300 or 400 MHz. Spectra are given in ppm (δ) and coupling constants, J, are reported in Hertz. Mass spectra were collected using a Waters ZQ
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144628010 vl Sing!e Quad Mass Spectrometer (ion trap electrospray ionization (ESI)). Purity and low resolution mass spectral data were measured using Waters Acquity i-class ultra-performance liquid chromatography (UPLC) system with Acquity Photo Diode Array Detector, Acquity Evaporative Light Scattering Detector (ELSD) and Waters ZQ Mass Spectrometer. Data was acquired using Waters MassLynx 4.1 software and purity characterized by UV wavelength 220 nm, evaporative light scattering detection (ELSD) and electrospray positive ion (ESI). (Column: Acquity UPLC BEH C18 1.7μιη 2.1 X 50 mm; Flow rate 0.6mL/min; Solvent A (95/5/0.1%: lOmM Ammonium Forraate/Acetonitrile Formic Acid), Solvent B (95/5/0.09%: Acetonitrile/Water/Formic Acid); gradient: 5-100% B from 0 to 2 mins, hold 100% B to 2.2 mins and 5% B at 2.21 mins. Preparatory HPLC purifications were conducted on a Waters SunFire CI 8 OBD Prep Column, lOOA, 5 μηι, 19 mm x 50 mm, Waters XBridge BEH C 18 OBD Prep Column, 130A, 5 μηι, 19 mm X 50 mm with UV detection (Waters 2489 UV/998 PDA), Waters SunFire C 18 OBD Prep Column, 100 A, 5 μηι, 19 mm x 150 mm. Waters XBridge BEH Shield RP18 OBD Prep Column, S 0Λ, 5 μη , 19 mm x 150 mm, or Waters X Select CSH CI S OBD Prep Column, 13θΑ, 5 μηι, 19 mm x 150 mm at 254 nm or 220 nm using a standard solvent gradient program (e.g., as designated below). The absolute configuration of the separated enantiomers of the compounds in the examples described herein were not determined. As such, the configuration of the resolved materials were arbitrarily assigned as R or S in each case.
[00260] Abbreviations used in the following examples and elsewhere herein are: atm atmosphere
BAST bis(2-methoxyethyl)aminosulfur trifluoride br broad
BIN AP (2;2'-bis(dipheny[phosphino)-l , 1 -bmaphthyl)
Figure imgf000100_0001
hexafluorophosph
Cbz carboxybenzyl d doublet
DABCO l,4-diazabicyclo[2.2.2]octane
99
144628010 vl DAST N",N-diethylaminosulfur trifluoride
DBU 2,3,4,6,7,8,9, 10-octahydropyrimido[l,2- ]azepine
DCE 1,2-dichloroethane
DCM dichloromethane
DEA diethyl amine
DIEA A N-diisopropylethylamine
DMA N,N-dimethylacetami de
DME 1,2-dimethoxy ethane
DMAP 4-dimethylaminopyridine
Figure imgf000101_0001
DMF-DMA iV,A-dimethy]fonnamide dimethyl acetal
DMSO dimethyl sulfoxide dppf 1 , 1 '-bis(diphenylphosphino)ferrocene
EA ethyl acetate
EDCI A'r~(3-dimethylaminopropyl)-A''-eth} carbodiimide hydrochloride
ESI electrospray ionization
FCC flash column chromatography h hour(s)
HATU [bis(dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-^]pyridinium 3- oxide hexafluorophosphate
HBTU 3-[bis(dimethylamino)meihyliumyl]-3H-benzotriazol-l-oxide hexafluorophosphate
HMPA hexamethylphosphoramide
HOBt benzotriazol-l-ol
HPLC high-performance liquid chromatography
100
4628010 vl LCMS liquid chromatography-mass spectrometry m multiplet
MHz megahertz min minutes
MPLC Medium pressure liquid chromatography
MTBE 2-methoxy-2-methylpropane μ\¥ microwave
NBS N-bromosuccinimide
NCS N-chlorosuccinimide
NMM 4-methylmorpholine
NMR nuclear magnetic resonance
PC}' 3 tricyclohexylphosphine
PE petroleum ether ppm parts per million q quartet
RT room temperature
RuPhos 2-dicyclohexylphosphino-2',6'-diisopropoxybiphenyl
RuPhos Pd 2nd Gen: Chloro(2-dicyclohexylphosphino-2',6'-diisopropoxy-l,r-biphenyl)[2-(2'- amino-1, 1 '-biphenyl)]palladium(II) s singlet
SCX strongly cationic exchange
SPhos 2-Dicyclohexylphosphino-2',6'-dimethoxybiphenyl t triplet
TBDMS ferf-butyldimethylsilyl
101
144628010 vl tBuBrettPhos di-te^butyl(2',4',6'-triisopropyl-3,6-dimethoxy-[l, -biphenyl]-2- yl)phosphine
TEA tri ethyl amine
TFA trifluoroacetic acid
THF tetrahydrofuran
TLC thin layer chromatography
TMSI trimethyisilyl iodide
TosMIC l-(isocyanomethylsulfonyl)-4-methylbenzene
XantPhos 4,5-Bis(diphenylphosphino)-9,9-dimethylxanthene
XPhos 2-Dicyclohexylphosphino-2',4',6'-triisopropylbiphenyl
Xphos 2nd generation precataiyst: Chloro(2-dicyclohexylphosphino-2',4',6'-triisopropyl-l, r- biphenyl)[2-(2'-amino-l, 1 '-biphenyl)]palladium(II)
Example 1: Intermediate 1: 3-Amino-6-methylthieno[2,3-6]pyridine-2-carboxylic aeid
Figure imgf000103_0001
Step 1 Step 2 Intermediate 1
Step 1. Ethyl 3-amino-6-methyIthieno[2,3-6]pyridine-2-carboxyIate
[00261] To a solution of 2-chloro-6-methylnicotinonitrile (1.00 g, 6.55 mmol) in DMF (10 ml) was added ethyl 2-mercaptoacetate (0,72 ml, 6.6 mmol) followed by a solution of potassium hydroxide (powder) ( 1.47 g, 26.2 mmol) in 3.0 mL of water at 0 °C. The reaction was stirred at 0 °C for 1 hour resulting in a precipitate. The precipitate was collected via filtration, washed with water (10 mL) followed by ether (10 mL) and then dried in vacuo to afford ethyl 3-amino- 6-methylthieno[2,3-6]pyridine-2-carboxylate as a yellow powder (1.3 g, 85%). LCMS (ESI, m/z): 237 [M-H] ' .
Step 2: 3-Amino-6-methylthieno[2,3-6]pyridine-2-carboxylic acid
102
144628010 vl [00262] To a solution of ethyl 3-amino-6-methylthieno[2,3-6]pyridine-2-carboxylate (2.30 g, 9.90 mmol) in methanol (15 mL) was added a solution of sodium hydroxide (2.00 g, 50,0 rnmoi) in water ( 5 mL). The resulting solution was stirred for 4 h at 70 °C. The resulting mixture was concentrated in vacuo, and the pH of the solution was adjusted to 6 with aqueous hydrogen chloride (1M) resulting in a precipitate. The solids were collected by filtration and dried in vacuo to afford 3-amino-6-methylthieno[2,3- >]pyridine-2-carboxylic acid as a yellow solid (1.2 g, 58%), LCMS (ESI, m/z) 209 [M+H]+
Example 2: Intermediate 2. 3-Amino-4-(difluoromethyl)-6-methylthieno[2,3-6]pyridine-2- carboxylic acid
Figure imgf000104_0001
Step 1. 4-(DifluoromethyI)-6-methyl-2-thioxo-l,2-dihydropyridine-3-carbonitrile
[00263] Into a 100-mL round-bottom flask was added l, l-difluoropentane-2,4-dione (2.00 g, 14.7 mmol), triethylamine (60 mg, 0.083 mL, 0.59 mmol), 2-cyanoethanethioamide (1.47 g, 14.7 mmol) and ethanol (30 mL), The reaction mixture was stirred for 2 h at 80 °C, resulting in a precipitate that was collected by filtration and dried in vacuo to afford 4-(difluoromethyl)-6- methyl~2 hioxo~l,2~dihydropyridine-3-carbonitrile as an orange solid (1.72 g, 58%). LCMS (ESI, m/z): 201 I'M- H] '
Step 2. Ethyl 3-amino-4-(difluoromethyl)-6-methylthieno [2,3-6] yridine-2-carboxylate
[00264] Into a 100-mL round-bottom flask was added 4-(difluoromethyl)-6-methyl-2- thioxo-l,2-dihydropyridine-3-carbonitrile (1.00 g, 4.99 mmol), ethyl 2-chloroacetate (0.610 g,
4.98 mmol), sodium methoxide (0.810 g, 14.99 mmol), and ethanol (30 mL). The resulting solution was stirred for 2 h at 80 °C and then concentrated in vacuo. The resulting crude product
103
144628010 vl was purified by FCC eluting with ethyl acetate/petroleum ether (2:3) to afford ethyl 3-amino-4- (diiluorornethyl)-6-meihylthieno[2,3-&]pyridine-2-carboxyiate as a yellow solid (580 mg, 41%). LCMS (ESI, m/z): 287 [M+H]+.
Step 3. 3-Amino-4-(difluoromethyl)-6-methylthieno [2,3-6] pyridine-2-carboxylic acid
[00265] Into a 100-mL round-bottom flask was added ethyl 3-amino-4-(difluoromethyl)-6- methylthieno[2,3-6]pyridine-2-carboxylate (0.300 g, 1.05 mmol), sodium hydroxide (0.210 g, 5.25 mmol) and ethanol (20 mL). The resulting solution was stirred for 1 h at 60 °C, concentrated in vacuo, and the pH was adjusted to 6 with hydrogen chloride (1 M). The solid product was collected by filtration and dried in vacuo to afford 3-amino-4-(difluoromethyl)-6- methylthieno[2,3-6]pyridine-2-cari)oxylic acid as a yellow solid (261 mg, 96%). LCMS (ESI, m/z): 259 ! \! ί!
Example 3: Intermediate 3, 3-Amino-4-(difluoromethy])-6-((2,4-diinethoxybenzyl)ainino) thieno [2,3-6] pyridine~2~carboxyIic acid
Figure imgf000105_0001
Intermediate 3
Step 1, 2-Chloro-4-(difluoromethyl)-6-((2,4-dimethoxybenzyl)amino)nicotinonitrile
[00266] Into a 100-mL round-bottom flask was added 2,6-dichloro-4-(difluoromethyl) pyridine-3-carbonitrile (2.00 g, 8.97 mmol), (2,4-dimethoxyphenyl)methanamine (6.00 g, 35.9 mmol), and ethanol (30 mL). The resulting solution was stirred overnight at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl
104
144628010 vl acetate/petroleum ether (1 : 1) to afford 2-chioro-4-(difluoromethyi)-6-((2,4- dimethoxybenzyi)amino)nicotinonitrile as a white solid (560 mg, 18%), LCMS (ESI, m/z) 354 | M Π Γ .
Step 2. Ethyl 3-amino-4-(difluoromethyl)-6-((2,4-dimethoxybenzyl)amino)thieno[2,3- b\ py r idine-2-carboxyIate
[00267] Into a 100-mL round-bottom flask was added 2-chloro-4-(difluoromethyl)-6-((2,4- dimethoxybenzyl)amino)nicotinonitrile (1.00 g, 2.83 mmol), potassium carbonate (1 .17 g, 8.42 mmol), ethyl 2-mercaptoacetate (340 mg, 2,83 mmol), and ethanol (25 mL). The reaction mixture was stirred overnight at 80 °C, concentrated in vacuo, and the resulting crude product purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford ethyl 3-amino-4- (difluoromethyl)-6-((2,4-dimethoxybenzyl)amino)thieno[2,3-Z>]pyridine-2-carboxylate as a yellow solid (980 mg, 79%). LCMS (ESI, m/z): 438 M ! !j .
Step 3. 3-Amino-4-(difluoromethyI)-6-((2,4-dimethoxybenzyI)amino)thieno[2,3-d]pyridine-
2-carboxy!ic acid
100268] Into a 100-mL round-bottom flask was added ethyl 3-amino-4-(difluoromethyl)-6- ((2,4-dimethoxybenzyl)amino)ihieno[2,3~^]pyridine~2-carboxylate (0,980 g, 2.24 mmol), sodium hydroxide (0.449 g, 11.2 mmol), ethanol (20 mL), and water (10 mL). The reaction mixture was stirred for 3 h at 80 °C and then cooled and concentrated in vacuo to remove the ethanol. The pH was adjusted to approximately 5 with 1 M aqueous HC1. The solid product was then collected by filtration to afford 3-amino-4-(difluoromethyl)-6-((2,4-dimethoxybenzyl) amino)thieno[2,3- £]pyridine~2-carboxylic acid as a yellow solid (500 mg, 55%). LCMS (ESI, m/z): 410 [M+H]+.
105
144628010 vl Example 4: Intermediate 4. 3-Antino-6-(( 'eri-butoxycarfoonyl)amino)-4-methylthieno[2,3- b]pyridine-2-carboxylic add
Figure imgf000107_0001
intermediate 4
Step 1. Methyl 3-amino-6-chloro-4-niethylthieno[2,3-6jpyridine-2-carboxylate
[00269] To a 250-mL round-bottom flask was added 2,6-dichloro-4-methylpyridine-3- carbonitrile (5,00 g, 26.7 mmol), methyl thioglycolate (2.93 mL, 32.1 mmol), and DMF (50 mL). A solution of potassium hydroxide (6.0 g, 106.9 mmol) in water ( 10 mL) was slowly added at 0 °C, and the resulting solution was stirred for 2 h at 0 °C. The reaction was quenched with addition of water (50 mL). The solid product was isolated by filtration, washed with water (10 mL), and dried in vacuo to afford methyl 3-amino-6-chloro-4-methylthieno[2,3-A]pyridine-2- carboxylate as a yellow solid (2,6 g, 38%). LCMS (ESI, m/z): 257 [M+H]+.
Step 2. Methyl 3-amino-6-((^r{:-butoxycarbonyl)amino)-4-niethylthieno[2,3-6]pyridine-2- carboxylate
[00270] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added methyl 3-amino-6-chloro-4-methylthieno[2,3-Z»]pyridine-2-carboxylate (1.00 g, 3,90 mmol), CS2CO3 (6.34 g, 19.5 mmol), teri-butyl carbamate (2,29 g, 19.6 mmol), XPhos 2nd generation precatalyst (0.771 g, 0.98 mmol), and dioxane (15 mL). The reaction mixture was stirred overnight at 90 °C and then concentrated in vacuo to provide a crude product purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford methyl 3-amino-6- ((te ^butoxycarbonyl)amino)-4-methylthieno[2,3-Z>]pyridine-2-carboxylate as a yellow solid (1.2 g, 91%). LCMS (ESI, m/z): 338 [M+H]+.
106
144628010 vl Steu 3. 3-Amino-6-((i£r^-butoxycarbonyl)aiiiino)-4--methyIthieno[2,3-6]pyridine--2-
Into a 50-mL round-bottom flask was added methyl 3-amino~6-[[(fen- butoxy)carbonyl]amino]-4-methylthieno[2,3-0]pyridine-2-carboxylate (0.600 g, 1.78 mmol), potassium hydroxide (0.498 g, 8.88 mmol), methanol (10 mL), and water (2 mL). The resulting suspension was stirred for 3 h at 60 °C upon which dissolution occurred. The reaction was cooled to RT which resulted in the formation of a precipitate. The pH was adjusted to approximately 6 with aqueous hydrogen chloride (3 M) which resulted in a thick precipitate. The solid product was collected by filtration, washed with water (10 mL), and dried in vacuo to afford 3-amino-6-((tm-butoxycarbonyl)amino)-4-methylthieno[2,3-Z>]pyridine-2-carboxylic acid as a yellow solid (200 mg, 35%). LCMS (ESI, m 'z): 324 [M l iJ . le 5: Intermediate 5, 3-Amino-4-methoxy-6-methylthieno[2,3- j]pyridine-2-
Figure imgf000108_0001
Step J. 2,4-DichSoro-6-niethyleicotinic acid
[00272] Into a 250-mL round-bottom flask was added ethyl 2,4-dichloro-6-methylpyridine-3- carboxylate (3.00 g, 12,8 mmol ), THF (30 mL), and water (30 mL) followed by the portion-wise addition of LiOH (1.23 g, 51.3 mmol). The resulting solution was stirred for 3 days at 85 °C and then concentrated in vacuo. The pH was adjusted to 3 with 4 N HC1 (aq) and the resulting
107
144628010 vl precipitate was collected by filtration and dried in vacuo to afford 2,4-dichloro-6-methylnicotinic acid as a white solid (3.0 g), The material was used without further purification. LCMS (ESI, m/z): 206 | M
Step 2. 2,4-DichSoro-6-niethyl icotioaniide
[00273] Into a 250-mL round-bottom flask was added 2,4-dichloro-6-methylnicotinic acid (2,00 g, 9.71 mmol) and dichloromethane (50-mL) followed by the portion-wise addition of oxaiyl chloride (6.35 g, 4.29 mL, 50.0 mmol). To this mixture was added DMF (50 mg, 0.053 mL) dropwise and with stirring. The resulting solution was stirred for 2 h at RT and then concentrated in vacuo to afford a crude product that was dissolved in dichloromethane (50-mL). To this solution was added NL Cl (2.12 g, 11.5 mmol) and triethylamine (10 g, 13.8 mL, 99.0 mmol) dropwise and with stirring. After stirring for 2 h at RT the reaction was quenched with 10 mL of water/ice and the resulting mixture was extracted with dichloromethane (3 x 30 mL). The organic layers were combined, washed with brine (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2,4-dichloro-6-methylnicotinamide as a white solid (2.1 g). The material was used without further purification. LCMS (ESI, m/z): 205 [M+H]+.
Step 3. 2,4-Dichloro-6-methylnicotinoiiitrile
[00274] Into a 3-mL round-bottom flask was added 2,4-dichloro-6-methylnicotinamide (200 mg, 0,98 mmol) and POC (3 mL), The reaction mixture was stirred for 2 h at reflux and then cooled and quenched with 10 mL of water/ice. The resulting solution was extracted with dichloromethane (3 x 30 mL) and the combined organic layers were washed with brine (30 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2,4-dichloro-6-methylnicotinonitrile as a white solid (150 mg, 82%), LCMS (ESI, m/z): I 87 [M+H]+.
Step 4. 2-Chloro-4-methoxy-6-methyhiicotinonitrile
[00275] Into a 250-mL round-bottom flask was added 2,4-dichloro-6-methylnicotinonitrile (1.00 g, 5,35 mmol) and methanol (100-mL) followed by the portion-wise addition of NaOMe (1.10 g, 20.4 mmol). The resulting solution was stirred for 4 h at reflux and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with 0 - 30%
108
144628010 vl ethyl acetate in petroleum ether to afford 2-chloro-4-methoxy-6-methylnicotinonitrile as a white solid (200 mg, 20%). LCMS (ESI, m/z) 183 [M+Hf .
Step 5. Methyl 3-amino-4-methoxy-6-methylthieno[2,3-6] pyridine-2-carboxylate
[00276] Into a 25 -mL round-bottom flask was added 2-chloro-4-methoxy-6- methylnicotinonitrile (0.260 g, 1.42 mmol), methyl thioglycolate (0.151 g, 1.42 mmol), and potassium hydroxide (0,320 g, 5,71 mmol), and DMF (5 mL) at 0 °C. The resulting solution was stirred for 1 h at 0 °C and then quenched with water (30 mL). The resulting solids were collected by filtration to afford methyl 3-amino-4-methoxy-6-methylthieno[2,3-6]pyridine-2-carboxylate as a red solid (340 mg). The material was used without further purification. LCMS (ESI, m/z): 253
Step 6, 3-Amino-4-methoxy-6-methylthieno[2,3-6]pyridine-2-carboxylic acid
[00277] Into a 50-mL round-bottom flask was added methyl 3-amino-4-methoxy-6- methylthieno 2,3-6]pyridine-2-carboxylate (0.110 g, 0.44 mmol), NaOH (0.035 g, 0.87 mmol), methanol (5 mL), and water (5 mL). The reaction mixture was stirred for 4 h at 80 °C and then concentrated in vacuo. The pH of the concentrated solution was adjusted to approximately 3 with aqueous HCl (3 M). The resulting precipitate was collected by fijtration and dried in vacuo to afford 3-amino-4-methoxy-6-methylthieno[2,3-6]pyridine-2-carboxylic acid as a brown solid (130 mg). The material was used without further purification, LCMS (ESI, m/z): 239 | \i · H j .
Example 6: Intermediate 6, 3-Amino-S-(difluoromethyl)thieno[2,3-6]pyridine-2-carboxylic acid
Figure imgf000110_0001
109
144628010 vl Step 1: 2-Ethylidenemalononitrile
[00278] Into a 250-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added lithium bromide (2,60 g, 29.9 mmol) and malononitrile (10.0 g, 151.37 mmol). The reaction mixture was stirred for 4 h at 80 °C and then acetaldehyde (13.0 g, 295 mmol) was added. The resulting solution was stirred for an additional 4 h at 80 °C and then cooled and diluted with aqueous saturated sodium bicarbonate (60 mL). The mixture was extracted with ethyl acetate (3 x 20 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2-ethylidenemalononitrile as a yellow oil (9 g, 65%). 1H»NMR (300 MHz, CDCh) δ ppm 7.46-7.39 (q, ./ 6 Hz, 1H), 2.30 (d, J = 6 Hz, 3H).
Step 2: 2-Chloro-5-formylnicotinonitrile
[00279] Into a 500-mL round-bottom flask was added phosphoryl trichloride (67.0 g, 437 mmol), followed by the dropwise addition of DMF (32.0 g, 33.8 mL, 1.52 mol) with stirring at RT. To this mixture was added 2-ethylidenemalononitrile (10.0 g, 109 mmol) dropwise. The resulting solution was stirred for 40 min at RT and then for 3 h at 95 °C. The reaction was cooled and then quenched with water/ice (500 mL). The pH of the solution was adjusted to approximately 7-8 with 60% aqueous sodium hydroxide. The resulting solution was extracted with ethyl acetate (3 x 100 mL), The combined organic layers were then washed with brine (2 x 500 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford 2- chloro-5-formylnicotinonitrile as a yellow oil (1.00 g, 6%). LCMS (ESI, m/z): 167 [M+H]+.
Step 3: 2~C loro~5~(diflnoromethy!)nieotiiiO!iitriIe
[00280] into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added 2-chioro-5-formylnicoti.nonitrile (1 ,00 g, 6,00 mmol) and dichloromethane (20 mL). The resulting solution was cooled to -78 °C and DAST (1.90 g, 1.56 mL, 51.2 mmol) was then added dropwise and with stirring. The reaction mixture was warmed and stirred for 60 min at 0 °C. The reaction was then warmed and stirred at RT overnight. The reaction mixture was quenched with water (100 mL) and was then extracted with dichloromethane (3 x 50 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC
110
144628010 vl eluting with ethyl acetate/petroleum ether (1 :5) to afford 2-chloro-5-(difluoromethyl) nicotinonitrile as a yellow solid (800 mg, 71%). LCMS (EST m/z): 189 [ M i l ] .
Step 4: Methyl 3-aniino-5-(difluoroinethyl)thieno[2,3-6]pyridine-2-carboxylate
[00281] Into a 50-mL round-bottom flask was added 2-chloro-5-(difluororaethy[) nicotinonitrile (0.500 g, 2.65 mmol) and DMF (5 mL). Methyl tbioglycolate (0.562 g, 5.29 mmol) was then added followed by NaOMe (0.429 g, 7.94 mmol). The resulting solution was stirred overnight at 60 °C and then cooled and diluted with water (50 mL). The mixture was extracted with ethyl acetate (3 x 20 mL). The combined organic layers were washed with brine (2 x 50 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting cmde product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :2) to afford methyl 3-amino-5-(difluoromethyl)thieno[2,3-b]pyridine-2-carboxylate as a yellow solid (300 mg, 44%). LCMS (ESI, m/z): 259 | \H i j \
Step 5: 3-Amino-5-(difluoromethyl)thieno[2,3-fi]pyridine-2-carboxylic acid
[00282] into a 100-mL round-bottom flask was added methyl 3-amino-5-(difluoromethy[) thieno[2,3-Z»]pyridine-2-carboxylate (0.500 g, 1.94 mmol) and methanol (20 mL). Water (2 mL) was then added, followed by potassium hydroxide (0.326 g, 5.81 mmol). The reaction mixture was stirred overnight at 60 °C and then cooled to RT. The pH of the solution was adjusted to approximately 6-7 with 1 M aqueous HO, The resulting solid precipitate was collected by filtration and dried in vacuo to afford 3-amino-5-(difluoromethyl)thieno[2,3-^]pyridine-2- carboxylic acid as a yellow solid (300 mg, 63%). LCMS (ESI, m/z): 245 j \! l ! j
111
144628010 vl Example 7: Intermediate 7, l-Amino-7,8-dihydro-6i -cycIopenta[ jthieno[2,3-d]pyridine-
2-earboxylie acid
Figure imgf000113_0001
Step 1. 2-Cyclopentylidenemalononitrile
[00283] Into a 250-rnL round-bottom flask was added c-yclopentanone (5.00 g, 59.4 mmol), malononitrile (5.90 g, 89.3 mmol), H4OAc (3.00 g, 39.0 mmol), HOAc (5 mL), and toluene (75 mL). The resulting solution was stirred overnight at 135 °C and then cooled and washed with H?0 (3 x 50 mL). The organic layer was separated and concentrated in vacuo to afford 2- cyclopentylidenemalononitrile as a light brown oil (7 g, 89%). LCMS (ESI, m/z): 133 [M+H]+.
Step 2. (£)-2-(2-((dimethylamino)methylene)cyclopentyIidene)malononitrile
[00284] Into a 100-mL round-bottom flask was added 2-cyclopentylidenemalononitrile (3.00 g, 22.7 mmol) and toluene (23 mL). Acetic anhydride (0.45 mL) was then added, followed by DMF-DMA (3.6 mL). The resulting solution was stirred for 30 min at RT and then diluted with hexane (30 mL). The resulting solids were collected by filtration and dried in vacuo to afford (£)-2-(2-((dimethylamino)methylene)cyclopentylidene)malononitrile as a yellow solid (3.2 g, 75%). LCMS: (ESI, m/z): 188 [M+H]+.
Step 3. 3-Chioro-6,7-dihydro-5fl-cyciopenta|"c]pyridine-4-carbonitrile
[00285] Into a 50-mL sealed tube was added (£)-2-(2-((dimethylamino)methylene) cyclopentylidene)malononitrile (3.00 g, 16,0 mmol), HOAc (10 mL), and 4 N hydrogen chloride/dioxane (20 mL). The solution was stirred overnight at 55 °C and was then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl
112
144628010 vl acetate/petroleum ether (1 :3) to afford 3-chloro-6,7-dihydro-5H-cyclopenta[c]pyridine-4- carbonitrile as a white solid. (2.8 g, 98%). LCMS (ESI, m/z): 179 [M+H]+.
Step 4. Methyl l-amino-7,8-dihydro-6jyr-cyclopenta[^]thieno[2,3-^]pyi'idine-2-carboxylate
[00286] Into a 1.00-mL round-bottom flask was added 3-chloro-6,7-dihydro-577- cyclopenta[c]pyridine-4-carbonitrile (1.00 g, 5.60 mmol), methyl thioglycolate (0.590 g, 0.500- mL, 5.60 mmol), potassium carbonate (2.31 g, 16,7 mmol), and DMF (20 mL). The reaction mixture was stirred overnight at 50 °C and then cooled and diluted with H20 (30 mL). The resulting solids were collected by filtration and dried in vacuo to afford methyl l-amino-7,8- dihydro-6if-cyciopenta[ii]thieno[2,3-/;]pyridine-2-carboxylate as an off-white solid (900 mg, 65%). LCMS (ESI, m/z): 249 | M I I I .
Step 5, l-Amino-7,8-dihydro-6H-cyclopenta[i lthieno[2,3-6]pyridine-2-carboxylic acid
[00287] The title compound was prepared according to the procedure used for the preparation of Example 6: Intermediate 6, using methyl l-amino-7,8-dihydro-6H"-cyclopenta[£¾thieno [2,3- #]pyridine-2-carboxylate and NaOH. This afforded the title compound as a light yellow solid (59%). LCMS (ESI, m/z): 235 [ M i l ] .
Example 8: Intermediate 8, 3-Amino-5,6-dimethyIthieno[2^-6]pyridine-2-carboxyIic acid
Figure imgf000114_0001
Step 1 SteP 2
intermediate 8
Step 1. Methyl 3-amino-5,6-dimethylthieno[2,3-6]pyridine-2-carboxylate
[00288] Into a 50-mL round-bottom flask was added 2-chloro-5,6-dimethylpyridine-3- carbonitrile (0.332 g, 1.99 mmol) and tetrahydrofuran (10 mL). Methyl thioglycolate (0.254 g, 0.214 mL, 2,40 mmol) was then added, followed by DBU (0.456 g, 0,447 mL, 3.00 mmol). The resulting solution was stirred for 2 h at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford methyl 3- amino-5,6-dimethylthieno[2,3-0]pyridine-2-carboxylate as a light yellow solid (400 mg, 85%). LCMS: (ESI, m/z) 237 [M+H]÷
Step 2. 3-A!Bisio-556-dimethylthieno[2,3-/j]pyrid5iie-2-carboxylic acid
113
144628010 vl [00289] Into a 50-mL round-bottom flask was added methyl 3-amino-5,6-dimethylthieno[2,3- Z»]pyridine-2-carboxylate (0,474 g, 2.01 mmol), a solution of sodium hydroxide (0.400 g, 10.00 mmol) in water (5 mL), and ethanol (5 mL). The solution was stirred for 2 h at 80 °C, concentrated in vacuo, and the pH of the solution was then adjusted to approximately 7 with aqueous HC1 (3 M). The resulting solid precipitate was collected by filtration and dried in vacuo to afford 3-amino-5,6-dimethylmieno[2,3-6]pyridine-2-carboxylic acid as a light yellow solid (400 mg, 90%), LCMS (ESI, m/z): 223 | \Η ί Γ
Example 9: Intermediate 9, 3-Amino-5-methylthieno[2,3-6]pyridine-2-carboxylic acid
Figure imgf000115_0001
Step 1 Step 2
Step 1. Methyl 3-amino-5-methyIthieno[2,3-d]pyridine-2-carboxyIate
[00290] Into a 100-mL round-bottom flask was added 2-chloro-5-methylpyridine-3- carbonitrile (0.500 g, 3.28 mmol) and DMF (5 mL). Potassium carbonate (0.908 g, 6.57 mmol) was then added followed by methyl thioglycolate (0.349 g, 0.294 mL, 3.29 mmol ) and the resulting solution was stirred for 2 h at RT. The reaction mixture was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1 ) to afford methyl 3-amino-5-methylthieno[2,3-£]pyridine-2-carboxylate as a yellow solid (700 mg, 96%). LCMS (ESI, m/z) 223 [M+H .
Step 2. 3-Aniino-5«inetliylt ieno[2,3-/j]pyridine-2-carboxylic acid
[00291] Into a 100-mL round-bottom flask was added methyl 3-amino-5-methylthieno[2,3- Z»]pyridine-2-carboxylate (0.650 g, 2,92 mmol), sodium hydroxide (1.17 g, 29.25 mmol), methanol (15 mL) and water (5 mL). The reaction mixture was stirred for 4 h at 50 °C and then concentrated in vacuo. The pH was adjusted to approximately 4 with aqueous HQ (2 M) and the resulting solid precipitate was collected by filtration and dried in vacuo to afford 3-amino-5- methylthieno[2,3-Z>]pyridine-2-carboxylic acid as a yellow solid (500 mg, 82%). LCMS (ESI, m/z) 209 [M+H]+.
114
144628010 vl Example 10: Intermediate 1( 3-Amino-6-(trifluoromethyI)thieno[2,3-^]pyridine-2-
Figure imgf000116_0001
Step 1 Step 2
Step 1, Methyl 3~afflino-6~(trifluoromethy!)thieiio[2,3~6]pyridiiie~2~carboxylate
[00292] The title compound was prepared according to the procedure used for the preparation of Example 6: Intermediate 6, using 2-chloro-6-(trifluoromethyl)nicotinonitrile. This afforded the title compound as a yellow solid (67%). LCMS (ESI, m/z) 277 [M+H]+.
Step 2. 3-Amino-6-(trifluoromethyl)thieno[2,3-6]pyridine-2-carboxylic acid
[00293] The title compound was prepared according to the procedure used for the preparation of Example 6: Intermediate 6, using methyl 3-amino-6-(trifluoromethyl)thieno[2,3- >]pyridine- 2-carboxylate. This afforded the title compound as a yellow solid (93%). LCMS (ESI, m/z); 263 m m .
Example 11: Intermediate 11. 3-Amino-6-methyl-4-(trifluoromethyl)thieno[2,3-6]pyridine-
2-carboxylic acid
Figure imgf000116_0002
Intermediate 11
Step ./. Ethyl 3-amino-6-methyI-4-(trifluoromethyl)thieno[2,3-d]pyridine-2-carboxylate
[00294] Into a 500-mL round-bottom flask was added 2-mercapto-6-methyl-4- (trifluoromethyl)nicotinonitrile (10.0 g, 45.8 mmol), ethyl 2-bromoacetate (7.66 g, 45.9 mmol) in EtOH (235 mL), and sodium eth oxide (4,67 g, 68.6 mrao!) and the resulting solution was heated and stirred for 2 h at reflux. The reaction was then cooled, quenched with water (100 mL), and the resulting mixture was extracted with DCM (3 x 100 mL). The combined organic layers were washed with brine (2 x 100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with DCM / MeOH (10: 1) to afford
115
144628010 vl ethyl 3-amino-6-methyl-4-(trifluoromethyi)thieno[2,3- >]pyridine-2-carboxyiate as a yellow solid (12 g, 86%), LCMS (ES, m/z): 305 [M÷H]+
Step 2. 3-Amino-6-methyl-4-(trifluoromethyl)thieno [2,3-6] yridine-2-carboxy lie acid
[00295] Into a 500-mL round-bottom flask was added ethyl 3-amino-6-methyl-4- (trifluoromethyl)thieno[2,3-6]pyridine-2-carboxylate (12.0 g, 39.4 mmol) and THF (200 mL). Water (15 mL) was added, followed by sodium hydroxide (6.58 g, 165 mmol). The resulting solution was heated and stirred for 5 h at 60 °C and then cooled. The pH was adjusted to approximately 2-3 with concentrated aqueous HQ and the resulting solid precipitate was filtered, and dried in vacuo to afford 3-amino-6-methyl-4-(trifluoromethyl)thieno[2,3-6]pyridine-2- carboxyiic acid as a yellow solid (9.4 g, 86%). LCMS (ESI, m/z): 277 j \ i I I I .
Example 12: Intermediate 12. 3-Amino-5-fluorothieno[2,3-6]pyridine-2-carboxylic acid
Figure imgf000117_0001
£ep S ep 2 intermediate 12
Step 1. Methyl 3-amino-5-fluorothieno[2,3-6]pyridine-2-carboxylate
[00296] Into a 50-mL round-bottom flask was added 2-chloro-5-fluoropyridine-3-carbonitrile
(0.400 g, 2.56 mmol) and DMF (4 mL). DIEA (0.495 g, 0.667 mL, 3.83 mmol) was then added followed by methyl thioglycolate (0, 136 g, 0, 1 15 mL, 1.28 mmol). The resulting solution was stirred overnight at RT and then quenched with water (40 mL) and extracted with ethyl acetate (3 x 10 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford methyl 3-amino-5-fluorothieno[2,3-i]pyridme-2- carboxylate as a yellow solid (1 10 mg, 19%). LCMS (ESI, m/z)'. 227 [M+H]+.
Step 2. 3-AnH!io-5-fluorothieno[2,3-/>]pyridme-2-carboxylic aeid
[00297] The title compound was prepared according to the procedure used for the preparation of Example 6: Intermediate 6, using methyl 3-amino-5-fluorothieno[2,3-6]pyridine-2- carboxylate and NaOH at 50 °C. This afforded the title compound as a yellow solid (66%). LCMS (ESI, m/z): 213 [M+Hf .
116
144628010 vl Example 13: Intermediate 13. 3-Amino-5-chlorothieno[2,3-6jpyridine-2-carboxyIic acid
Figure imgf000118_0001
Ste -J Step 2 intermediate 13
Step 1. Methyl 3-amino-5-chlorothieno[2,3-6] pyridine- 2-carboxylate
[00298] The title compound was prepared according to the procedure used for the preparation of Example 12: Intermediate 12, using 2,5-dichloronicotinonitrile and potassium carbonate. This afforded the title compound as a yellow solid (84%). LCMS (ESI, rn/z): 243 ( M i l l
Step 2. 3-Amino-5-chlorothieno[2,3-6]pyridine-2-carboxylic add
[00299] Into a 100-mL round-bottom flask was added methyl 3-amino-5-chlorothieno[2,3- £]pyridine-2-carboxylate (1.40 g, 5,77 mmol) and tetrahydrofuran/HiO (3/1 ; 15 mL). NaOH (1.20 g, 30.0 mmol) was then added and the resulting solution was stirred overnight at RT. The pH of the solution was adjusted to approximately 6 with aqueous HQ (2 M) and the resulting solid precipitate was collected by filtration and dried in vacuo to afford 3-amino-5- chlorothieno[2,3-6]pyridine-2-carboxylic acid as a yellow solid (1.1 g, 83%). LCMS (ESI, m/z) 229 [M+H]÷.
Example 14: Intermediate 14. 3-Amino-6-(hydroxyniethyI)thieno[2,3-6]pyridine-2-
Figure imgf000118_0002
Step S
Intermediate 14
Step 1. 2-Chloro-3-cyano-6-methylpyridine l-oxide
[00300] Into a 50-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 2-chloro-6-methylpyridine-3-carbonitrile (0.200 g, 1.31 mmol), /ra-CPBA (0.339 g), and dichioromethane (6 mL). The solution was stirred overnight at
117
144628010 vl 35 °C then concentrated in vacuo. The resulting crude product was purified by FCC eluting with petroleum ether ; ethyl acetate (3 : 1) to afford 2-chloro-3-cyario-6-methylpyridine 1 -oxide as a white solid (65 rag, 29%). LCMS (ESI, m/z): 169 [M+H]+.
Step 2. (6-Chloro-5-cyanopyridin-2-yl)methyl acetate
[00301] Into a 100-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added a solution of 2-chloro-3-cyano-6-methylpyridine 1 -oxide (0.250 g, 1.48 mmol), sulfuric acid (catalytic amount, approximately 1 drop), and acetic anhydride (6 mL). The solution was stirred tor 3 h at 1 10 °C, and then cooled and stirred overnight at RT. The reaction mixture was quenched with water (40 mL) and extracted with ethyl acetate (30 mL). The organic layer was washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (9: 1) to afford (6-chloro-5-cyanopyridin-2- yl)methyl acetate as a yellow oil (190 mg, 61%). LCMS (ESI, m/z) 211 | M · l i |
Step 3. 2-Chloro-6-(hydroxymethyl)nicotinonitrile
[00302] Into a 50-mL round-bottom flask was added (6-chloro-5-cyanopyridin-2-yl)methyl acetate (0.190 g, 0,90 mmol), potassium carbonate (0.187 g, 1 ,35 mmol), and methanol (2 mL), The reaction mixture was stirred for 3 h at RT and then concentrated in vacuo. Water (10 ml) was added and the resulting solution was extracted with ethyl acetate (3 x 10 mL). The combined organic layers were washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2-chloro-6-(hydroxymethyl)nicotinonitrile as a yellow oil (120 mg). The material was used without further purification. LCMS (ESI, m/z): 369 m m .
Step 4. Methy! 3-amino-6-(hydroxymethyl)thieno[2,3-¾]pyridme-2-carboxylate
[00303] The title compound was prepared according to the procedure used for the preparation of Example 8; Intermediate 8, using 2-chloro-6-(hydroxymethyl)nicotinonitrile. This afforded the title compound as a yellow solid (71 %). LCMS (ESI, m/z): 239 [M Π .
Step 5. 3-Amino-6-(hydroxymethyI)thieno[2,3-i]pyridine-2-carboxylic acid
[00304] The title compound was prepared according to the procedure used for the preparation of Example 6: Intermediate 6, using methyl 3-amino-6-(hydroxymethyl)thieno[2,3-A]pyridine-
118
144628010 vl 2-carboxylate and LiOH. This afforded the title compound as a yellow solid (49%). LCMS (ESi,
Figure imgf000120_0001
Example 15: Intermediate 1: 3-Amino-5-fluoro-6-methylthieno[2,3-6]pyridine-2-
Figure imgf000120_0002
intermediate 15
Step 1. Methy! 3-amiiio~5-ili!oro~6~methylthieiio [2,3-6] pyridine-2-carboxylate
[00305] Into a 50-mL round-bottom flask was added 2-chloro-5-fluoro-6-methylpyridine-3- carbonitrile (1.00 g, 5.86 ramo!), DMF H2O (15 mL/15 mL), methyl 2-mercaptoacetate (1 .25 g, 1.05 mL, 11.8 mmol), and KOH (0.990 g, 17.64 mmol). The resulting solution was stirred for 3 h at RT then extracted with ethyl acetate (3 x 200 mL). The combined organic layers were concentrated in vacuo. The crude product was purified via silica gel column chromatography and e!uted with ethyl acetate/petroleum ether (1 :4) to afford methyl 3-amino-5-iluoro-6- methylthieno[2,3-6]pyridine-2-carboxylate as a yellow solid (0.8 g , 57%). LCMS (ESI, m/z): 241
2, 3~Amino-5-fliioro-6~fflethylthieno 2,3~6]pyridi85e~2-carboxySic acid
[00306] The title compound was prepared according to the procedure used for the preparation of Example 12, Intermediate 12, using methyl 3-amino-5-fluoro-6-methylthieno[2,3- ft]pyridine-2-carboxylate at RT. This afforded the title compound as a yellow oil (89%), LCMS (ESI, m/z): 227 j \ i ! i j".
Example 16: Intermediate 16. 3-Amino-6-ethyIthieno[2,3-0]pyridine-2-carboxyIic acid
119
144628010 vl
Figure imgf000121_0001
intermediate 16
Steu l. 2-C
[00307] Into a 500-mL round-bottom flask was added 2-chloro-6-methylpyridine-3- carbonitrile (8.00 g, 52.4 mmol) and D F (200 mL), The resulting mixture was cooled to 0 °C and sodium hydride (60% dispersion in mineral oil; 3.00 g, 125 mmol) was added portion-wise. The reaction mixture was stirred for 10 mill and then iodomethane (29,6 g, 13.0 mL, 209 mmol) was added dropwise with stirring. The resulting solution was stirred for 2 h at RT and then quenched with H2O (500 mL). The reaction mixture was extracted with ethyl acetate (3 x lOOmL), and the combined organic layers were washed with brine. The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate / petroleum ether (1 :5) to afford 2-chloro-6- ethylnicotinonitrile as a yellow oil (4 g, 46%). LCMS (ESI, m/z): 168 [M+Hf .
Step 2. Ethyl 3-amino-6-ethylthieno[2,3-6]pyridine-2-carboxylate
[00308] Into a 100-mL round-bottom flask was added 2-chloro-6-ethylnicotinonitrile (2.00 g, 12.0 mmol), DMF (50 mL), potassium carbonate (5.00 g, 35.9 mmol), ethyl 2-mercaptoacetate (1.35 g, 1.23 mL, 11.2 mmol). The resulting solution was stirred overnight at 80 °C and then cooled and extracted ethyl acetate (with 3 x 150 mL). The combined organic layers were washed with brine (3 x 100-mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :3) to afford ethyl 3-amino-6-ethylthieno[2,3-i]pyridine-2- carboxylate as a yellow solid (1.8 g, 63%). LCMS (ESI, m/z) 251 ( \! ! i j .
Step 3. 3-Amino-6-ethylthieno[2,3-ft]pyridine-2-carboxylic acid
120
144628010 vl [00309] into a 100-mL round-bottom flask was added ethyl 3-amino-6-ethylthieno[2,3- Z»]pyridine-2-carboxylate (0.800 g, 3.20 mmol), ethanol (20 mL), and THF (20 mL). This was followed by the dropwise addition of a solution of sodium hydroxide (0,384 g, 9.60 mmol) in water (10 mL) with stirring. The resulting solution was stirred for 3 h at 50 °C. The resulting mixture was concentrated in vacuo. The pH of the solution was adjusted to approximately 3-5 with aqueous HC1 (3 M). The resulting precipitate was collected by filtration to afford 3-amino- 6-ethylthieno[2,3-£]pyridine-2-carboxylic acid as a yellow solid (600 mg, 80%) that was carried on without further purification. LCMS (EST m/z): 223 j \l i I j ' .
Example 17: Intermediate 17. 3-Amino-6-methoxythieno[2,3-6]pyridine-2-carboxylic acid
Figure imgf000122_0001
Intermediate 17
Step 1. 2-Chloro-6-methoxynicotinonitrile
[00310] Into an oven dried 250-mL round-bottom flask under a dry nitrogen atmosphere was added methanol (25 mL) followed by sodium metal (1.5 g, 65 mmol). After the metal had dissolved, 2,6-dichloropyridine-3-carbonitrile (5.00 g, 28.9 mmol) was then added over 5 rain. The resulting solution was stirred overnight at RT. The solid precipitate was then removed by filtration, and the filtrate was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/hexane ( 1 :3 ) to afford 2-chloro-6-methoxynicotinonitrile as a white solid (3.9 g, 80%). LCMS (ESI, m/z): 168 | \ ! Π Γ.
Step 2. Methyl 3-aniino-6-methoxythieno[2,3-6]pyridine-2-carboxyIate
[00311] The title compound was prepared according to the procedure used for the preparation of Example 5: Intermediate 5, using 2-chloro-6-methoxynicotinonitrile. This afforded the title compound as a light yellow solid (51%). LCMS (ESI, m/z): 239 [M+H]+; ' l l-NMR (300 Ml !/,
121
144628010 vl DMSO-i¾) δ ppm 8.40 (d, J = 8.7 Hz, 1H), 7.24 (m, 2H), 6.89 (d, J = 9.0 Hz, i l l ). 3.93 (s, 3H), 3.77 (s, 3H).
Step 3. 3-Amino-6~methoxythieno [2,3-6] pyridine-2-carboxylic acid
[00312] Into a 50-mL round-bottom flask was placed methyl 3-amino-6-methoxythieno[2,3- 6]pyridine-2-carboxylate (0.1 10 g, 0.46 mmol), tetrahydrofuran HzO (4 mL/1.5 mL), and LiOH (0, 100 g, 4, 18 mmol). The resulting solution was stirred for 2 h at 60 °C and then concentrated in vacuo to remove most of the THF. The resulting mixture was diluted with water (2 mL). The pH of the solution was adjusted to approximately 7 with aqueous HCl (IM). The solid product was isolated by filtration to afford 3-amino-6-methoxythieno[2,3-6]pyridine-2-carbox>4ic acid as a yellow solid (90 mg, 87%) that was carried on without further purification. LCMS (ESI, m/z): 225
Example 18; Intermediate 18. 3-Amino-5-fluoro-6-methoxythieno[2,3-6]pyridine-2- carboxylic acid
Figure imgf000123_0001
intermediate 18
Step ./. 2-Chloro~5-fluoro-6-methoxynicotinonitriIe
[00313] Into a 100-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 2,6-dichloro-5-fIuoropyridine-3-carbonitrile (3.00 g, 15.7 mmol), methanol (30 mL), and sodium methoxide (1.28 g, 23.7 mmol). The resulting solution was stirred for 5 h at RT and then concentrated in vacuo. The caide product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :3) to afford 2- chloro-5-fluoro-6-methoxynicotinonitrile as a yellow solid (2,9 g) that was carried on without further purification. LCMS ( LSI, ;,v .:): 187 | M 1 1 j .
122
144628010 vl Step 2. Methyl 3-amino-5-fluoro-6-methoxythieno [2,3-6] yridine-2-carboxylate
[00314] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added 2-chloro-5-fluoro-6-methoxynicotinonitrile (2.94 g, 15,8 mmol), tetrahydrofuran (30 mL), methyl 2-mercaptoacetate (2.00 g, 1.68 mL, 18.8 mmol), and DBU (7.20 g, 7.07 mL, 47.3 mmol). The resulting solution was stirred overnight at RT and then concentrated in vacuo. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (EA/PE = 1/3) to afford methyl 3-amino-5-fluoro-6- methoxythieno[2,3-6]pyridine-2-carboxylate as a yello solid (1.87 g, 46%). LCMS (ESL m/'∑) 257 [ \! ! i |
Step 3, 3-Amino-5-fluoro-6-methoxythieno[2,3-6]pyridine-2-carboxylic acid
[00315] Into a 100-mL round-bottom flask was added methyl 3-amino-5-fluoro-6- methoxythieno[2,3-6]pyridine-2-carboxyiate (0.500 g, 1.95 mmol), LiOH (0.236 g, 9.85 mmol), tetrahydrofuran (8 mL), and water (8 mL). The resulting solution was stirred overnight at 60 °C in an oil bath and then cooled to RT. The resulting mixture was concentrated in vacuo to remove the THF. The pH of the resulting mixture was adjusted to approximately 7 with aqueous HCl (4 M). The resulting solids were collected by vacuum filtration and dried in vacuo to afford 3- amino-5-fluoro-6~methoxythieno[2,3-6]pyridine-2~carboxylic acid as a yellow solid (260 mg, 55%). LCMS (ESI, m/z): 243 [M+H]÷.
Example 19: Intermediate 19. 3-Amino-6-ethyl-5-fluorothieno[2,3-6]pyridine-2-carboxylic acid
Figure imgf000124_0001
intermediate 3
Step 1, 2~ChIoro~6~ethyI-5~fl oroeicotiiiOiiitriIe
123
144628010 vl [00316] into a 250-mL 3 -necked round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 2,6-dichloro-5-fluoropyridine-3-carbonitrile (5.00 g, 26.2 mmol), potassium phosphate tribasic (5.60 g, 26.4 mraol), trietbylborane (26,0 niL 28.80 mmol), Pd(dppf)Ch (1.10 g, 1.50 mmol), and THF (100-mL). The reaction mixture was stirred overnight at 70 °C and then cooled to RT and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford 2-chloro-6-ethyl- 5-fluoronicotinonitrile as a white solid (3.2 g, 66%). LCMS (ESI, m/z) 185 [M+H]+.
Step 2. Ethyl 3-amino-6-ethyl-5-fluorothieno [2,3-6] pyridine-2-carboxylate
[00317] Into a 100-mL round-bottom flask was added 2-chloro-6-ethyl-5-fluoronicotinonitrile (3.00 g, 16.3 mmol) and THF (40 mL). Sodium, methoxide (2,63 g, 48,7 mmol) was added followed by ethyl thioglycolate (2.19 g, 2,00 raL, 18,2 mmol), The resulting solution was stirred overnight at 70 °C. The reaction mixture was cooled to RT and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford ethyl 3-amino-6-ethyl-5-fiuorothieno[2,3-6]pyridine-2-carboxylate as a yellow solid (2.3 g, 53%). LCMS (ESI, m/z) 269 [M+H]+
Step 3. 3-Amino-6-ethyl-5-fluorothieno[2,3-6]pyridine-2-carboxylic acid
[00318] The title compound was prepared according to the procedure used for the preparation of example 11: Intermediate 11 using ethyl 3-amino-6-ethyl-5-fluorothieno[2,3-6]pyridine-2- carboxylate and EtOH which was added as a cosolvent. This afforded the title compound as a yellow solid (89%). LCMS (ESI, m/z) 241 [M+Hf.
Example 20: Intermediate 20, 3~Amino-6-chloro-4-methylthieno[2,3-6]pyridine-2- carboxylic acid
Figure imgf000125_0001
Ethyl 3-amino-6-chloro-4-methylthieno[2,3-d]pyridine-2-carboxylate
124
144628010 vl [00319] Ethyl 2-mercaptoacetate (0.586 mL, 5.35 mmol) was added to a solution of 2,6- dichloro-4-methylnicotinonitrile (1.00 g, 5.35 mmol) in DMF (10 mL) at -5 °C This was followed by the slow addition of an aqueous solution of potassium hydroxide (powder, 1.20 g, 21.4 mmol) in water (2.5 mL). The reaction mixture was stirred at -5 °C for 1 hour (an orange precipitate forms). Water (5 mL) was added and the resulting precipitate was collected by vacuum filtration. The precipitate was washed with water (10 mL), collected, and dried in vacuo to afford ethyl 3-amino-6-chloro-4-methylthieno[2,3-d]pyridine-2~carboxylate as a pale orange powder (980 mg, 68%). LCMS (ESI, m/z): 271 ( \i f i j .
Step 2, 3-Amino-6-chloro-4-methyIthieno [2,3-6] pyridine-2-carboxyIic acid
[00320] To a suspension of ethyl 3-amino-6-chloro-4-methylthieno[2,3-i]pyridine-2- carboxylate (0.970 g, 3 ,58 mmol) in ethanol (8 ml) was added water (4.00 nil) followed by potassium hydroxide (powder; 1.00 g, 17.9 mmol). The reaction mixture was stirred at 50 °C for 3 hours (white precipitate forms). The reaction was cooled to RT and acidified to approximately pH = 5 with aqueous IN HC1 (the solution turns clear and then a white precipitate forms again). The precipitate was collected by filtration, washed with water (10 mL) and ether (10 mL), and dried in vacuo to afford 3-amino-6-chloro-4-methylthieno[2,3-Z»]pyridine-2-carboxylic acid as a white powder (560 mg, 64%). LCMS (ESI, m/z): 243 [M+H]+.
Example 21: Intermediate 21. 3-Amino~6-chlorothieno[2 -^]pyridine-2~carboxylic acid
Figure imgf000126_0001
stgP 1 SieP 2 intermediate 21
Step 1. Ethyl 3-amino-6-chlorothieno[2,3- ]pyridine-2-carboxylate
[0032 J] The title compound was prepared according to the procedure used for the preparation of Example 20: Intermediate 20, using 2,6-dichloronicotinonitrile. This afforded ethyl 3- amino-6-chlorothieno[2,3-&]pyridine-2-carboxyiate as a light yellow solid (85%). LCMS (ESI, m/z): 257 [ M i l ] ' .
Step 2, 3-Amino-6-chlorothieno[2,3-fi]pyndine-2-carboxylic acid
125
144628010 vl [00322] The title compound was prepared according to the procedure used for the preparation of Example 20: Intermediate 20, using ethyl 3-amino-6-chlorothieno[2,3- ]pyridine-2- carboxy!ate. This afforded the title compound as a light yellow solid (98%). LCMS (ESI, nv'z): 229 j \i l i j
Example 22: Intermediate 22. 3-Amino-5-bromo-6-methylthieno[2,3-2>]pyridine-2- car boxy lie acid
Figure imgf000127_0001
intermediate 22 Step 4
Step 1. 5-Bromo-6-methyI-2-oxo-l,2-dihydropyridine-3-carbonitrile
[00323] A mixture of 6-methyl-2-oxo-l ,2-dihydropyridine-3-carbonitrile (4.00 g, 29.8 mmol) and NBS (11.2 g, 62.6 mmol) in DCE (150 rnL) was heated to reflux overnight. The reaction was cooled to RT and the resulting precipitate was collected via vacuum filtration. The precipitate was suspended in water (330 mL), stirred for 2 hours, and then collected via vacuum filtration. The filter cake was washed with water (50 rnL), collected, and dried in vacuo to afford 5-bromo-6-methyl-2-oxo-l,2-dihydropyridine-3-carbonitrile as a white powder (5.3 g, 84%). Ή NMR (300 MHz, OMSO-de) δ ppm 12.99 (br s, H I ). 8.36 (s, H i t. 2.35 (s, 31 !)
Step 2. 5-Bromo~2-chIoro-6-methylnicotinonitrile
[00324] 5-bromo-6-methyl-2-oxo-l ,2-dihydropyridine-3-carbonitrile (2.00 g, 9,39 mmol) was dissolved in phosphoryl trichloride (1.5 ml, 16, 1 mmol) at RT, Phosphorous pentachlonde (1.96 g, 9.39 mmol) was then added and the resulting mixture was heated to 110 °C overnight. The reaction mixture was cooled to RT and concentrated in vacuo. The crude product was purified by
126
144628010 vl FCC during with 5 - 25% EtOAc in hexanes to afford 5-bromo-2-chloro-6-methylnicotinoriitrile as a light yellow solid (2.1 g, 97%). ¾ NMR (300 MHz, DMSO-ifc) δ ppm 8,80 (s, 1H), 2.63 (s, 3H),
Step 3. Ethyl 3-amino-5-bromo-6-methylthieno [2,3-6] pyridine-2-carboxylate
[00325] To a solution of 5-bromo-2-chloro-6-methylnicotinonitrile (0.81 g, 3.5 mmol) in DMF (10 mL) at 0 °C was added ethyl 2-mercaptoacetate (0.422 g, 0.384 mL, 3,50 mmol), followed by the slow addition of a solution of potassium hydroxide (powder; 0.785 g, 14.0 mmol) in water (2.5 mL). The reaction mixture was stirred at 0 °C for 1 hour (an orange precipitate forms). Water (5 mL) was then added and the resulting precipitate was collected by filtration, washed with water (10 mL), collected, and dried in vacuo to afford ethyl 3-amino-5- bromo-6-methylthieno[2,3-Z>]pyridine-2-carboxylate as a pale orange powder (1 ,0 g, 95%). LCMS (ESI, m/z): 316 [M+Hf.
Step 4. 3-Amino-5-bromo-6-methylthieno[2,3-6]pyridine-2-carboxylic acid
[00326] The title compound was prepared according to the procedure used for the preparation of Example 21: Intermediate 21, using ethyl 3-amino-5-bromo-6-methylthieno[2,3- »]pyridine-
2-carboxy!ate. This afforded the title compound as a white solid (93%). LCMS (ESI, m/z): 288 [M+H]+
Example 23: Intermediate 23: 3-Amino-6-(difluoromethyl)-4-methylthieno[2,3-6]pyridine- 2-carboxylic acid
Figure imgf000128_0001
Intermediate 23
[00327] The title compound was prepared according to the procedure used for the preparation of Example 22: Intermediate 22, using ethyl 3-amino-6-(difluoromethyl)thieno[2,3-6]pyridine- 2-carboxylate. This afforded the title compound as a white solid (99%). LCMS (ESI, m/z): 259 [ M H i .
127
144628010 vl Example 24: Intermediate 24: Ethyl 3-aniino-0-((2,4-dimethoxybenzyl)ainino)-5- fluor othieno [2,3-6] py ridine-2-carboxyIate
Figure imgf000129_0001
Step 1. 2-Chloro-6-((2,4-dimethoxybenzyI)aniino)-5-fluoronicotinonitrile
[00328] Into a 250-mL round-bottom flask was added 2,6-dichloro-5-fluoropyridine-3- carbonitrile (5.00 g, 26.2 mmol), (2,5-dimethoxyphenyl)methanamine (13.0 g, 77,8 mmol), and ethanol (100 mL). The solution was stirred for 2 h at RT. The resulting solids were collected by filtration and dried in vacuo to afford the title compound as a white solid (6.6 g, 78%). LCMS (ESI, m/z): 322 | \1
Step 2. Ethyl 3-amino-6-((2,4-dimethoxybenzyl)amino)-5-fIuorothieno[2,3-6]pyridine-2- carboxyiate
[00329] Into a 100-mL round-bottom flask was added 2-chloro-6-((2,4-dimethoxybenzyl) amino)-5-fluoronicotinonitrile (2.00 g, 6.22 mmol), ethyl 2-mercaptoacetate (0.745 g, 0.680 mL, 6,20 mmol), potassium carbonate (2.57 g, 18.5 mmol), and ethanol (50 mL). The reaction mixture was stirred overnight at 70 °C and then cooled to RT and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford ethyl 3-amino-6-((2,4-dimethoxybenzyl)amino)-5-fluorothieno[2,3-^]pyridine-2- carboxylate as a light yellow solid (1.5 g, 60%). LCMS (ESI, m/z): 406 [ M i l ]
128
144628010 vl Example 25: Intermediate 25. Benzyl 4-(4-(2-aniinoethyI)phenyl)piperazine-l-carboxylate
(hydrochloride salt)
Figure imgf000130_0001
Step 1, terf-Butyl (4-bromophenethyl)carbamate
[00330] To a solution of 2-(4-bromophenyl)ethan-l -amine (5.00 g, 25.0 mmol) in anhydrous dichlorometha e (50-raL) was added B0C2O (6,57 g, 30.1 mmol) followed by EtaN (10.4 mL, 74.9 mmol). The resulting solution was stirred overnight at 25 °C and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (PE/EA=3 : 1) to afford fert-butyl (4-bromophenethyl)carbamate as a white solid (7.1 g, 95%). LCMS (ESI, m/z) 300 [M+H]+.
Step 2. Benzyl 4-(4-(2-((½/ -butoxycarbonyl)amino)ethyl)phenyl)piperazine-l-carboxylate
[00331] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added fe/t~buty! (4~bromophenethyl)carbarnate (4.00 g, 13.3 mmol) dissolved in anhydrous toluene (50-mL). To the resulting solution was added benzyl piperazine-1- carboxylate (3.53 g, 16.0 mmol), Pd(OAc)2 (0.300 g, 1.34 mmol), XPhos (1.28 g, 2.69 mmol), and CS2CO3 (13.1 g, 40.0 mmol). The reaction mixture was stirred overnight at 105 °C in an oil bath and then cooled to RT and quenched with H2O (200 mL). The resulting mixture was extracted with ethyl acetate (2 x 50 mL). The combined organic layers were washed with brine (1 x 200 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (PE/EA=3: 1) to afford benzyl 4-(4-(2-((feri-butoxycarbonyl)amino)ethyl)phenyl) piperazine-1- carboxylate as a yellow solid (5 g, 85%), LCMS (ESI, m/z): 440 [M+H]+.
129
144628010 vl Step 3. Benzyl 4-(4-(2-aminoethyI)phenyI)piperazine-l-carboxylate (hydrochloride salt)
[00332] Into a 100-mL round-bottom flask was added benzyl 4-(4-(2-((tm- butoxycarbonyl)amino)ethyl)phenyl)piperazine-l-carboxylate, followed by 4 N hydrogen chloride/dioxane (10 mL). The resulting solution was stirred for 1 h at RT and then concentrated in vacuo to afford benzyl 4-(4-(2-aminoethyl)phenyl)piperazine-l-carboxylate (hydrochloride salt) as a yellow solid (2.0 g, 86%). LCMS (ESI, m z' ): 340 | M i ! i .
Example 26: Intermediate 26. 2-(4-(4-Cyclobutylpiperazin-l-yl)phenyl)ethan-l-amine
(hydrochloride salt)
Figure imgf000131_0001
intermediate 28
Step 1. teri-Butyl (4-(piperazin-l-yI)phenethyl)carbamate
[00333] Into a 50-mL high pressure reactor under an inert atmosphere of nitrogen was added benzyl 4-[4-(2-[[(feri-butoxy)carbonyl]amino]ethyl)phenyl] piperazine-l-carboxylate (1.00 g, 2,28 mmol) and 10% palladium on carbon (0.1 g), followed by methanol (15 mL). The reactor was then pressurized with hydrogen (10 atni), and the resulting raixture was stirred for 16 h at RT. The reaction mixture was vented to nitrogen and the soiids were removed by filtration through Celite. The resulting filtrate was concentrated in vacuo to provide fer -butyl (4- (piperazin- 1 -yl)phenethyl)carbamate as a yellow oil (0.67 g, 96%). LCMS (ESL m zy' . 306 i xf i ί I
Step 2. tertAhm l (4-(4-cyclobutylpiperazin-l-yl)phenethyl)carbamate
[00334] Into a 100-mL round-bottom flask was added tert-butyl (4-(piperazin-l - yl)phenethy!)carbainate (0.700 g, 2.29 mmol), cyclobutanone (0.320 g, 4.57 mmol), and ZnCb (0.610 g, 4.47 mmol), followed by DCE (15 mL). NaBHsCN (0,290 g, 4.61 mmol) was then
130
144628010 vl added and the resulting mixture was stirred for 16 h at 50 °C. The reaction mixture was concentrated in vacuo and the crude product was purified by FCC eluting with DCM:MeOH (3 : 1) to afford ferf-butyl (4-(4-cyclobutylpiperazin-l-y3)phenethyl)carbamate as a yellow oil (0.5 g, 61%). i CMS (ESI, m/z): 360 j M I ! ] .
Step 3, 2~(4-(4~CycIobislylpiperazi85-l-yI)phei5yl)ethasi-l~ams!¾e hydrochloride
[00335] Into a 25-mL round-bottom flask was added ferf-butyl (4-(4-cyclobutylpiperazin-] - yi)pheiiethyl)carbamate (0.500 g, 1.39 mmol) followed by HCl/'dioxane (4 N, 10 ml). The resulting solution was stirred for 6 h at RT and the solid product formed was collected by filtration to afford 2-(4-(4-cyclobutylpiperazin-l-yl)phenyl)ethan-l -amine (hydrochloride salt) as a light yellow solid (0.3 g, 73%). I CMS (ESI, m/z) 260 [M+H]~.
Example 27: Intermediate 27, Benzyl 4-(4-(2-aminoethyl)-3-methoxyphenyl)piperazine-l- carboxylate (hydrochloride salt)
Figure imgf000132_0001
* Step 3
Figure imgf000132_0002
Step 1. Benzyl 4-(4-bromo-3-methoxyphenyl)piperazine-l-carboxylate
[00336] Into a lOG-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added l,4-dibromo-2-methoxybenzene (2.60 g, 9.78 mmol), benzyl piperazine- 1-carboxylate (2.37 g, 10.8 mmol), Pd2(dba)3-CHCij (0.508 g, 0.490 mmol), XantPhos (0,583 g, 0.980 mmol), and NaOtBu (2.82 g, 29.3 mmol) followed by toluene (40 mL). The reaction mixture was stirred, for 3 h at 80 °C and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-
131
144628010 vl bromo-3-methoxyphenyl)piperazine-l-carboxylate as a brown solid (2 g, 50%). LCMS (ESi, m/z): 405, 407 [M÷H]+
Step 2, Benzyl 4-(4-(2-((ieri-butoxycarbonyl)amino)ethyl)-3-methoxyphenyl)piperazine-l- carboxylate.
[00337] Into a lOQ-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added benzyl 4-(4-bromo-3-methoxyphenyl)piperazine-l-carboxylate (0,500 g, 1.23 mmoi), potassium (2-((tert-butoxycarbonyl)amino)ethyl) tnfluoroborate (0.308 g, 1.22 mmoi), Pd(dppf)Cl2 (0,050 g, 0.07 mmoi), RuPhos (0.057 g, 0, 12 mmoi), CS2CO3 (1.19 g, 3.65 mmoi), toluene (10 mL), and water (3 mL). The resulting solution was stirred for 5 h at 95 °C in an oil bath and then concentrated in vacuo. The crude product was purified via silica gel chromatography and eiuted with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-[4-(2- [[(benz}doxy)carbonyl]amino]ethyl)-3-methoxyphenyi]piperazine-l-carboxylate as a brown solid (540 mg, 87%), LCMS (ESI, m/z): 470 \\ - \ \ \ '
Step 3. Benzyl 4-(4-(2-aminoethy!)-3-methoxyphenyl)piperazine-i-carboxy!ate
(hydrochloride salt)
[00338] Into a 100~mL round-bottom flask was added benzyl 4-[4-(2-[[(tert- butoxy)carbonyl]amino]ethyl)-3-methoxyphenyl]piperazine-l-carboxylate (0.470 g, 1 .00 mmoi), and dioxane (5 mL). HCl gas was bubbled into the reaction mixture and the resulting solution was stirred for 2 h at RT. The reaction mixture was concentrated in vacuo to afford benzyl 4-[4- (2-aminoethyl)-3-methoxyphenyl]piperazine-l-carboxylate (hydrochloride salt) as a white solid (400 mg, 98%) that was carried on without further purification. LCMS (ESI, m/z): 370 [M+H]+.
132
144628010 vl Example 28: Intermediate 28, Benzyl 4- -am
-carboxylate (hydrochloride salt)
Figure imgf000134_0001
Ste S
Step 1. Benzyl 4-(4-nitro~2~(trifluoromethyl)phenyl)piperazine~l~carboxylate
[00339] into a 100-mL round-bottom flask was added l-fiuoro-4-nitro-2- (trifluoromethyl)benzene (3.20 g, 15.3 mmol) followed by DMSO (20 n L). Benzyl piperazine-
1 - carboxylate (3.30 g, 15.0 mmol) and CS2CO3 (15.0 g, 46.0 mmol) were then added and the reaction mixture was stirred overnight at 120 °C. The reaction mixture was cooled to RT, quenched with water (100 mL), and extracted with ethyl acetate (3 x 15 mL). The combined organic layers were concentrated in vacuo to afford a crude product that purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-nitro-2- (trifluoromethyl)phenyl)piperazine-l -carboxylate as an orange oil (3.2 g, 51%). LCMS (ESI, m/z . 410 ! M-H] .
Step 2. 4-(Piperazin-l-yl)-3-(trifluoromethyl)aniline
[00340] into a 50-mL round-bottom flask purged with nitrogen was added benzyl 4-(4-nitro-
2- (trifluoromethyl)phenyl)piperazine-l -carboxylate (2.00 g, 4.89 mmol) followed by methanol (20 mL). 10% Palladium on carbon (200 mg) was added and the reaction was purged with hydrogen using a hydrogen filled balloon (subsurface bubbling). The reaction mixture was stirred for 2 h under a hydrogen atmosphere, vented to nitrogen, and the solids were removed by
133
144628010 vl filtration over Celite. The filtrate was then concentrated in vacuo to afford 4-(piperazin-l-yl)-3- (trifluoromethyl)aniline as a black oil (1.0 g, 83%). LCMS (ESI, m/z): 246 i\j-H ]
Step 3. l-(4-Bromo-2-(trifluoromethyl)phenyl)piperazine
[00341] Into a 50-mL round-bottom flask was added 4-(piperazin-l-yl)-3- (trifluoromethyl)aniline (1.30 g, 5.30 mmol), CuBr (0.400 g, 2.79 mmol), aqueous HBr (48% by weight; 4.2 mL), and water (15 raL). A solution of NaN(>2 in water H2O (1.6 g, 23.2 mmol in 2 mL) was then added dropwise at 0 °C and the resulting reaction mixture was stirred for 2 h at 60 °C. The pH of the solution was adjusted to approximately 8 with aqueous sodium hydroxide (2 M) and then extracted with ethyl acetate (3 x 15 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford a cmde product that purified by FCC elating with ethyl acetate/petroleum ether (1 : 1) to afford l -(4-bromo-2- (trifluoromethyl)phenyl)piperazine as a yellow oil (613 mg, 37%). LCMS (ESI, m/z): 309 [ Π Γ .
Step 4. Benzyl 4-(4-bromo-2-(trifluoromethyl)p enyl)piperazine-l-carboxyIate
100342] Into a 50-mL round-bottom flask was added l-[4-bromo-2-(trifluoromethyl)phenyl] piperazine (0.520 g, 1.68 mmol) followed by dichlorom ethane (10 mL). The resulting mixture was cooled to 0 °C and benzyl chloroformate (0.347 g, 0.290 mL, 2.03 mmol) was then added followed by triethylamine (0.515 g, 0,709 mL, 5.09 mmol). The resulting solution was stirred for 3 h at 0 °C and then concentrated in vacuo. The crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford benzyl 4-(4-bromo-2-(trifluoromethyl)phenyl) piperazine-l-carboxylate as a colorless oil (410 mg, 55%). LCMS (ESI, m/z): 443 I I 1 1 j .
Step 5, Benzyl 4-(4-(2-((i'crt-butoxycarbonyl)ainino)ethyl)-2-(trifluoromethyl)phenyl) piperazine-l-carboxylate
[00343] into a 50-mL round-bottom flask was added benzyl 4-(4-bromo-2- (trifluoromethyl)phenyl)piperazine-l-carboxylate (0.410 g, 0.92 mmol) followed by a mixture of toluene and H2O (8 mL/ 2 mL). Potassium (2-((fenf-butoxycarbonyl)amino)ethyJ) trifiuoroborate (0.251 g, 1.00 mmol) was then added followed by CszCC (0.912 g, 2.80 mmol) and Pd(dppf)Ch (70 mg, 0.10 mmol). The reaction mixture was purged with nitrogen (subsurface bubbling for 5 minutes) and then stirred for 3 h at 90 °C. The resulting mixture was concentrated in vacuo and
134
144628010 vl the crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford benzyl 4~(4-(2-((&T -butoxycarbony
carboxylate as a yellow oil (363 mg, 77%). LCMS (ESI, m/z): 508 j M 1 11 .
Step 6. Benzyl 4-(4-(2-aminoethyl)-2-(trifluoroniethyI)phenyl)piperazine-l-carboxylate hydrochloride
[00344] Into a 50-raL round-bottom flask was added benzyl 4-(4-(2-((terf-butoxycarbonyl) amino)ethyl)-2-(trifluoromethyl)phenyl)piperazine-l -carboxylate (0.400 g, 0.79 mmol). 4 N HCl in dioxane (10 rnL) was then added and the resulting solution was stirred for 2 h at RT. The reaction mixture was concentrated in vacuo to afford the title compound as a light yellow solid (302 mg, 94%). LCMS (ESI, z): 408 | \i H |
Example 29: Intermediate 29, Benzyl 4-(4-bromo-2-methylphenyI)piperazine-l- carboxyiate
Figure imgf000136_0001
Step I, Benzyl 4-(2-methyl-4-nitrophenyl)piperazine-l-carboxylate
[00345] Into a 100-mL round-bottom flask was added l-fluoro-2-methyl-4-nitrobenzene (1.55 g, 9.99 mmol), benzyl piperazine-l-carboxylate (2,20 g, 9,99 mmol) and CS2CO3 (9.78 g, 30.0 mmol) followed by DMSO (15 rnL). The resulting suspension was stirred for 2 h at 140 °C and then quenched with water (50 rnL) and extracted with ethyl acetate (3 x 100-mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to
135
144628010 vl afford benzyl 4-(2-methyl-4-nitrophenyl)piperazine-l-carboxylate as an orange solid (2.1 g, 59%). LCMS (ESI, m/z) 356 [M+Hf.
Step 2. 3-Methyl-4-(piperazin-l-yl)aniline
[00346] Into a 250-mL round-bottom flask was added benzyl 4-(2-methyl-4- nitrophenyl)piperazine-l-carboxylate (4.16 g, 1 1.7 mmol), 10% palladium on carbon (400 mg), and methanol (120 mL) under an atmophere of nitrogen. The reaction mixture was purged with a hydrogen-filled balloon (subsurface bubbling) and then stirred overnight at RT. The reaction was vented to nitrogen and the solids were removed by filtration through Celite. The filtrate was concentrated in vacuo to afford 3-methyl-4-(piperazin-l-yi)aniline as an orange solid (2.22 g, 99%). LCMS (ESI, m/z): 192 ΓΜ+Η]+
Step 3, l-(4-Bromo~2~methylphenyI)piperazine
[00347] Into a 50-mL round-bottom flask was added 3-methyl-4-(piperazin-l -yi)aniline (1.12 g, 5.86 mmol), aqueous HBr (48% by weight, 5.23 g), and aNC (647 mg, 9.38 mmol) in water (9 mL). To another 50-mL round-bottom flask charged with CuBr (1.53 g, 10.7 mmol) in water (7 mL) and aqueous HBr (48% by weight, 4.75 g), was added the former solution dropwise. The resulting solution was stirred for 2 h at 60 °C, quenched with water (20 mL), and extracted with ethyl acetate (3 x 100-mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford l-(4-bromo-2-methylphenyl)piperazine as a purple solid (630 mg, 42%). LCMS (ESI, m/z): 255 | \ί ! ! | .
Step 4, Benzyl 4-(4-bromo-2-methylphenyl)piperazine-l-carboxylate
[00348] Into a 250-mL round-bottom flask was added l-(4-bromo-2-methylphenyl)piperazine (0.630 g, 2.47 mmol), Ets (0.758 g, 1.04 mL, 7.49 mmol), and dichloromethane (80 mL). This was followed by the dropwise addition of benzyl chloroformate (0.468 g, 0.390 mL, 2.74 mmol) with stirring. The resulting solution was stirred for 1 h at 0 °C in a water/ice bath and then concentrated in vacuo. The crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-bromo-2-methylphenyl)piperazine-l- carboxylate as a brown oil (680 mg, 71%). LCMS (ESI, m/z): 389 f f ! I j
136
144628010 vl Example 30: Intermediate 30. tert-Batyl 4-{4-brorao-2-(difiiioroinethoxy)phenyI)
Figure imgf000138_0001
intermediate 30
Step 1, l-Bromo-2-(di'
[00349] Into a 250-mL round-bottom flask, purged and maintained under an inert, atmosphere of nitrogen, was added 2-bromo-5-nitrophenol (4.65 g, 21.3 mmol), ethyl 2-chloro-2,2- difluoroacetate (4.50 g, 28.4 mmol), and potassium carbonate (2.9 g, 21 mmol) followed by DMF (30 mL). The reaction mixture was stirred overnight at 70 °C in an oil bath and then quenched with water (50 mL). The resulting solution was extracted with ethyl acetate (3 x lOOmL), the organic layers combined, dried over anhydrous sodium sulfate filtered, and concentrated in vacuo to afford l-bromo-2-(difluoromethoxy)-4-nitrobenzene as a red oil (5.68 g, 99%), LCMS (ESI, m/z): 268 i'M-H] .
Step 2. Benzyl 4-(2-(difluoromethoxy)-4-nitrophenyl)piperazine-l-carboxylate
[00350] Into a 250-mL round-bottom flask was added l-bromo-2-(difluoromethoxy)-4- nitrobenzene (5.68 g, 21.2 mmol), benzyl piperazine-l-carboxylate (5.61 g, 25.5 mmol), and cesium carbonate (8.80 g, 26.9 mmol) followed by DMF (20 mL). The reaction mixture was heated and stirred for 3 h at 140 °C and then quenched with water (50 mL). The reaction mixture was extracted with ethyl acetate (2 x 100 mL), the combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford benzyl 4-(2-
137
144628010 vl (difluoromethoxy)-4-nitrophenyl)piperazine-l-carboxylate as a red solid (4.8 g, 56%). LCMS (ESI, m/z): 408 [M-H]
Step 3. 3-(Difluoromethoxy)-4-(piperazin~l-yl)aniline
[00351] Into a 250-mL round-bottom flask that was purged with nitrogen was added benzyl 4- (2-(difluoromethoxy)-4-nitrophenyl)piperazine-l-carboxylate (4.80 g, 11.8 mmol), 10% palladium on carbon (500 nig) and methanol ( 100 mL). The reaction mixture was sparged with hydrogen gas (subsurface bubbling) and was then stirred overnight under hydrogen (balloon) at RT. The reaction mixture was filtered over Celite, and the filtrate was concentrated in vacuo to afford 3-(difluoromethoxy)-4-(piperazin-l-yl)aniline as an orange solid (2.8 g, 98%). LCMS (EST m/z): 244 j M Π ] .
Step 4, l-(4-Bromo~2~(difluoromethoxy)phenyl)piperazine
[00352] Into a 250-mL round-bottom flask was added 3-(difluoromethoxy)-4-(piperazin-l - yi)aniline (2.80 g, 11.5 mmol), aqueous HBr (48% by weight 10 g), NaNC (1.27 g, 18.4 mmol) and water (12 mL). The resulting solution was carefully added to a solution of CuBr (2,99 g) in aqueous HBr (48% by weight, 9.6 g). The reaction mixture was stirred for 2 h at 60 °C and then quenched with water (50 mL). The pH of the solution was adjusted to approximately 7 with sodium hydroxide (1 M). The resulting solution was extracted with dichlorom ethane (3 x lOOmL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford l-(4-bromo-2-(difluoromethoxy)phenyl)piperazine as a brown oil (2 g, 57%). LCMS (ESI, m z): 307 [M+Hf.
Step 5, ter^-Butyl-4-(4-bromo-2-(difluoromethoxy)phenyl)piperazine-l-carboxylate
[00353] Into a 250-mL round-bottom flask was added l -(4-bromo-2- (difluoromethoxy)phenyI)piperazine (2.00 g, 6.51 mmol), Boc?.0 (1.70 g, 7.79 mmol) and dichloromethane (50 mL). Triethylamine (1,98 g, 19.6 mmol) was added and the resulting solution was stirred for 2 h at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford tert-butyl 4-(4- bromo-2-(difluoromethoxy)phenyl)piperazine-l-carboxylate as a white solid (2.5 g, 94%). LCMS (ESI, m/z): 407 [M+Hf.
138
144628010 vl Example 31-1: intermediate 31-1. Benzyl 4-(4-bromo-2-chlorophenyl)piperazine-l-
Figure imgf000140_0001
intermediate 31-1
[00354] Into a 100-mL round-bottom flask that purged and maintained under an inert atmosphere of nitrogen was added 4-bromo-2-chloro-l-iodobenzene (1.20 g, 3.78 mmol), benzyl piperazine-l -carboxylate (0.924 g, 4, 19 mmol), NaOtBu (1.10 g, 1 1.4 mmol), XantPhos (0.695 g, 1.20 mmol), Pd2(dba)3 (0.393 g, 0.430 mmol), and toluene (10 mL). The reaction mixture was stirred for 4 h at 60 °C and then concentrated in vacuo to provide a crude product that was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-bromo-2- chlorophenyl)piperazine-l -carboxylate as a colorless oil (713 mg, 46%). LCMS (ESI, m/z) 409, 41 1 [ M 1 [ j .
[00355] The Intermediates in Table 1 below were synthesized according to the procedures (palladium caialyzed C-N coupling) outlined above for Example 31-1, Intermediate 31-1, using the appropriate synthetic precursors.
Table 1:
Figure imgf000140_0002
139
144628010 vl
Figure imgf000141_0001
romo- , - uorop eny azet n- - an tert- uiy -azet n- -
140
144628010 vl MS (ESI.
Intermediate No.: Preciirsors Used (Notes) nt/z) yl)carbaraate yljcarbamate
Intermediate 31-15: ferf-Butyl 3-((4- l,4-Dibromo-2,5-difluorobenzene
bromo-2,5-difluorophenyl)amino)azetidine- and te/7-butyl 3-aminoazetidine-l- 363, 365
1-carboxylate carboxylate (reaction temp: 70 °C)
Example 32: Intermediate 32, Benzyl 4-(4-bromo-2,6-difluorophenyl)piperaziiie-l- carboxylate
Figure imgf000142_0001
intermediate 32
Step 1, Benzyl 4-(2,6-difluoro-4-nitrophenyl)piperazine-l-carboxylate
[00356] Into a 500-mL round-bottom flask was added l,2,3-trifluoro-5-nitrobenzene (3,00 g, 16,9 mraoi), benzyl pjperazine-1 -carboxylate (3,70 g, 16,8 mmol), potassium carbonate (5,85 g, 42.3 mmol), and MeCN (200 mL). The reaction mixture was stirred overnight at 60 °C and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with PE/EA (1/1) to afford benzyl 4-(2,6-difluoro-4-nitrophenyl)piperazine-l -carboxylate as a yellow solid. LCMS (ESI, m/∑): 378 [M+H]+.
Step 2. Benzyl 4-(4-amino-2,6-difluorophenyl)piperazine-l-carboxylate
[00357] into a 250-mL round-bottom flask was added benzyl 4-(2,6-difluoro-4- nitrophenyl)piperazine-l -carboxylate (1 ,60 g, 4.24 mmol), iron dust (1.6 g), and acetic acid (25 mL). The reaction mixture was stirred for 3 h at 80 °C and then cooled to RT. The solids were removed by filtration over Celite and the filtrate was diluted with EtOAc (250-mL). The organic solution was then washed with 10% aqueous sodium bicarbonate solution (1 x 300 mL), followed by brine (100-mL). The organic layer was dried over sodium sulfate, filtered, and
141
144628010 vl concentrated in vacuo to afford benzyl 4-(4-amino-2,6-difluorophenyi)piperazine-l-carboxylate as a yellow solid (1.2 g, 81%). LCMS (ESI, m/z): 348 [M+H]+.
Step 3. Benzyl 4-(4-bromo-2,6-difluorophenyl)piperazine-l-carboxylate
[00358] The title compound was prepared according to the procedure used for the preparation of Example 28: Intermediate 28, except benzyl 4-(4-amino-2,6-difluorophenyl)piperazine-l- carboxylate was used in place of 4-(piperazin-l-yl)-3-(trifluoromethyl)aniline. The title compound was obtained as a yellow solid (57%). LCMS (ESI, m/z): 411 [M+H]+.
Example 33: Intermediate 33. Benzyl 4-(4-(2-((ferf-butoxycarbonyl)amino)ethyl)-2-chloro- 6-fluorophenyl)piperazine-l-carboxylate
Figure imgf000143_0001
Step 1
Step 1. Benzyl 4-(4-(2-((ieri-butoxycarbonyl)amino)ethyl)-2-fluorophenyI)piperazine-l- car boxy late
[00359] Into a 250-mL 3 -necked round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added benzyl 4-(4~bromo-2-fluorophenyl)piperazine~ 1 - carboxylate (Intermediate 31-3, 3.20 g, 8.14 mmoi), potassium (2-((tert- butoxycarbonyl)amino)ethyl) trifluoroborate (2.45 g, 9.76 mmo!), Pd(dppf)Ch (0.670 g, 0.92 mmol), cesium carbonate (7.94 g, 24.3 mmoi), RuPhos (0.760 g, 1.63 mmol), toluene (90 mL) and water (30 mL). The reaction mixture was stirred for 4 h at 95 °C and then cooled and concentrated in vacuo. The resulting cmde product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford benzyl 4-(4-(2-((te/f-butoxycarbonyl)amino)ethyl)-2- fluorophenyi) piperazine-1 -carboxylate as a light yellow solid (2.2 g, 59%). LCMS (ESI, m/z): 458 | \! I i |
142
144628010 vl Step 2. Benzyl 4-(4-(2-((teri-butoxycarbonyl)aniino)ethyl)-2-chIoro-6-fluorophenyl) piperazine-l-carboxylate
[00360] Into a 100-mL round-bottom flask was added benzyl 4-(4-(2-((ferf-butoxycarbonyl) amino)ethyl)-2-fluorophenyl)piperazine-l-carboxylate (1.00 g, 2.19 mmol), dichloromethane (40 mL), and NCS (0.293 g, 2.19 mmol). The resulting solution was stirred overnight at reflux and then concentrated in vacuo to afford the crude product. The caide product was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4- (2-((ferf-butoxycarbo as yellow oil (350 mg, 33%). LCMS (ESI, m/z): 492 j M I i } .
Example 34: Intermediate 34. Benzyl 4-(4-(2-((ferf-butoxycarbonyI)amino)ethyI)-2- chlorophenyl)piperazine-l-carboxyiate
Figure imgf000144_0001
[00361] The title compound was prepared according to the procedure used for the preparation of Example 33: Intermediate 33, except benzyl 4-(4-(2-((fert-butoxycarbonyl)amino)ethyl) phenyl)piperazine-l-carboxylate was used in place of benzyl 4-[4-(2-[[(tert-butoxy)carbonyl] amino]ethyl)-2-fluoroph.enyl]piperazine-l -carboxylate. This afforded the title compound as a yellow solid (57%). LCMS (ESI, m/z): 474 [M+Hf.
Example 35: Intermediate 35. Benzyl 7-bromo-3,4-dihydroisoquinoline-2(lH)-carboxylate
Figure imgf000144_0002
[00362] Into a 250-mL round-bottom flask was added a 7 -bromo- 1 ,2,3,4- tetrahydroisoquinoline (2.00 g, 9.43 mmol) and dichloromethane (30 mL). Triethylamine (4.80 g, 6.61 mL, 47.4 mmol) was added followed by benzyl chloroform ate (1.61 g, 1.34 raL, 9.44 mmol) and the resulting solution was stirred for 1 h at RT. The reaction mixture was concentrated in vacuo and the crude product was purified by FCC eluting with ethyl
143
144628010 vl acetate/petroleum ether (1 : 1) to afford benzyl 7-bromo-3,4-dihydroisoquinoline-2(lH)- carboxylate as a colorless oil (2.7 g, 83%). LCMS (ESI, m/z) 346 [M+H]+.
Example 36: Intermediate 36, Benzyl 4-(4-bromo-2-cycIopropylphenyl)piperazine-l- carboxylate
Figure imgf000145_0001
Intermediate 36
Step 1. Benzyl 4-(2-bromo-4-nitrophenyl)piperazine-l-carboxylate
[00363] Into a 250-mL round-bottom flask was added 2-bromo-l-fluoro-4-nitrobenzene ( 10.0 g, 45.5 mmol), benzyl piperazine-l-carboxylate (20.0 g, 90.8 mmol), potassium carbonate (12.6 g, 90.5 mmol) and DSViSO (300 mL). The resulting mixture was stirred for 2 h at 70 °C and then cooled, diluted with H2O (100 mL), and extracted with ethyl acetate (3 x 150 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford benzyl 4-(2-bromo-4-nitrophenyl)piperazine-l-carboxylate as yellow oil that was carried on without further purification (20 g crude: 54%). LCMS (ES, m/z): 420, 422 | \i ! i | .
Step 2. Benzyl 4-(2-cyclopropyl-4-nitrophenyl)piperazine-l-carboxylate
[00364] Into a 500-mL 3 -necked round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added benzyl 4-(2-bromo-4-niirophenyl)piperazine-l- carboxylate (8.00 g, 19.0 mmol), cyclopropylboronic acid (6.55 g, 76.3 mmol), Pd(()Ac)2 (0.21 g, 0.95 mmol), PCv3 (0.536 g, 1.90 mmol), potassium phosphate tribasic (14.1 g, 66.43 mmol), toluene (200 mL), and water (10 mL). The reaction mixture was stirred for 2 h at 100 °C and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting
144
144628010 vl with ethyl acetate/petroleum ether (1 :3) to afford benzyl 4-(2-cyclopropyl-4- nitrophenyl)piperazine-l-carboxylate as a yellow solid (6.6 g, 91%). LCMS (ESI, m/z): 382 | M Π Γ .
Step 3. Benzyl 4-(4-amino-2-cyclopropylphenyl)piperazine-l-carboxylate
[00365] Into a 250-mL round-bottom flask was added a benzyl 4-(2-cyclopropyl-4- nitrophenyl)piperazine-l-carboxylate (3.60 g, 9.44 ramol), anhydrous tin (II) chloride (8.54 g, 45.0 mmol), ethanol (100-mL), and water ( 10 mL). The reaction mixture was stirred for 3 h at 80 °C and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate to afford benzyl 4-(4-amino-2-cyclopropylphenyl)piperazine-l- carboxylate as a yellow solid (3 g, 90%). LCMS (ESI m/z) 352 I M Π ] .
Step 4, l-(4-Bromo-2-cyclopropylphenyl)piperazine.
[00366] Into a 100-mL 3-necked round-bottom 11 ask was added benzyl 4-(4-amino-2- cyclopropylphenyl)piperazine-l-carboxylate (2.00 g, 5.69 mmol) and aqueous HBr (48%, 20 mL). This was followed by the addition of a solution of sodium nitrite (0.430 g, 6,23 mmol) in water (5 mL) dropwise with stirring at 0 °C. The resulting solution was stirred for 30 min at 0 °C and then a solution of cuprous bromide (1.7 g, 11 ,85 mmol) in aqueous HBr (48%, 20 mL) was added dropwise with stirring at 0 °C. The resulting soiution was stirred for 1 h at 60 °C. The pH of the solution was adjusted to approximately 8-9 with aqueous sodium hydroxide (2 M) and then the solution was extracted with ethyl acetate (3 x 100 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford l-(4-bromo-2-cyclopropylphenyl)piperazine as a yellow solid (1 g, 62%). LCMS (ES, m/z) 281, 283 [ M I I ] .
Step 5. Benzyl 4-(4~bromo-2-cyclopropyIpheiiyl)piperazioe-l-carboxyIate
[00367] Into a 50-mL round-bottom flask was added l-(4-bromo-2- cyclopropylphenyl)piperazine (1.00 g, 3,56 mmol) and tetrahydrofuran (15 mL), This was followed by the addition of a solution of sodium carbonate (1.13 g, 13.4 mmol) in water (5 mL) dropwise with stirring. To the reaction mixture was added benzyl chloroformate (0.730 g, 0.608 mL, 4.28 mmol) dropwise with stirring at 0 °C, The resulting solution was warmed and stirred
145
144628010 vl for 1 h at RT and then washed with H2O (2 x 10 mL). The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-bromo-2- cyclopropylphenyl)piperazine- 1 -carboxylate as a yellow solid (1.1 g, 74%). LCMS (ESI, m/z): 414, 416 [M+Hf. ά le 37: Intermediate 37. Benzyl
cycIopropylphenyI)piperazine-l-carboxylate
Figure imgf000147_0001
Intermediate 37
Step 1. 2-(2-Bromo-4-nitrophenyl)ethaii-l-amine
[00368] Into a 500-mL round-bottom flask was added 2-(4-nitrophenyl)ethan-l -amine (8.30 g, 50.0 mmol), NBS (9.79 g, 55,0 rnrnol), and sulfuric acid (cone, 200 mL). The resulting solution was stirred for 2 h at 50 °C and then cooled to RT, and quenched with water (200 mL). The pH of the solution was adjusted to approximately 7 with aqueous sodium hydroxide (1 M). The resulting solution was extracted with dichloromethane (3 x 300 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2- (2-bromo-4-nitrophenyl)ethan-l-amine as an off-white oil (12 g, 98%). LCMS (ESI, m/z): 245 j \H i | \
146
144628010 vl Step 2. iert~B ty\ (2-bromo-4-nitrophenethyI)carbainate
[00369] Into a 500-mL round-bottom flask was added 2-(2-bromo-4-nitrophenyl)ethan-l- amine (12.0 g, 49.0 mmol) and dichloromethane (250-mL). B0C2O (1 1.0 g, 50.40 mmol) was added followed by triethylamine (15.0 g, 20.7 mL, 149 mmol), and the resulting solution was stirred for 2 h at T. The reaction mixture was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford tert-butyl (2-bromo-4-nitrophenethyl)carbamate as a yellow solid (13 g, 77%). LCMS (ESI, m/z): 345
I M m .
Step 3, tert- utyl (2-cyclopropyl-4-nitrophenethyl)carbamate
[00370] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added ter/-butyl (2-bromo-4-nitrophenethyl)carbamate (3.44 g, 9.97 mmol), cyclopropylboronic acid (1.72 g, 20.0 mmol), Pd(dppf)C (0.732 g, 1.00 mmol), CS2CO3 (9.78 g, 30.0 mmol), toluene (20 mL), and water (2 mL). The reaction mixture was stirred overnight at 100 °C then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford tert-butyi (2-cyclopropyl-4- nitrophenethyi)carbamate as an orange solid (2.8 g, 91%). LCMS: (ESI, m/z): 307 | X i i I {
Step 4. tert-Butyl (4-amino-2-cyclopropylphenethyl)carbamate
[00371] Into a 250-mL round-bottom flask was added fert-butyl (2-cyclopropyl-4- nitrophenethyl)carbamate (1.40 g, 4.57 mmol), iron powder (2.8 g, 50.1 mmol), and AcOH (20 mL). The reaction mixture was stirred for 2 h at 40 °C and then quenched with water (100-mL). The pH of the solution was adjusted to approximately 7 with aqueous sodium bicarbonate. The mixture was extracted with dichloromethane (3 x 300 mL). The combined organic layers were then dried over sodium sulfate, filtered, and concentrated in vacuo to afford tert-butyl (4-amino- 2-cyclopropylphenethyl)carbamate as a brown oil (1.1 g, 87%). LCMS (ESI, m/z): 277 [M+H]+.
Step 5. tert-Butyl (2-cycIopropyl-4-(piperazin-l-yl)phenethyl)carbamate
[00372] Into a 250-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-butyl (4-amino-2-cyclopropylphenethyl)carbamate (1.10 g, 3.98 mmol), bis(2-chloroethyl)amine hydrochloride (1.10 g, 6.16 mmol; Caution: toxic), potassium carbonate (1.70 g, 12.3 mmol), and »-BuOH (20 mL). The reaction mixture was stirred
147
144628010 vl overnight at 100 °C in an oil bath and then cooled and concentrated in vacuo to afford fert-butyl (2-cyclopropyl-4~(piperazin-l-yl)phenethyl)carbamate as a brown solid (1.2 g, 87%), LCMS (ESI, m/z): 346 [M+H]+.
Step 6. Benzyl 4-(4-(2-((tert-butox carbonyI)amino)ethyl)-3-cyclopropylphenyl)piperazine- 1-carboxylate
[00373] Into a 250-mL round-bottom flask was added ferf-butyl (2-cyclopropyl-4-(piperazin- l-yl)phenethyl)carbamate (1.20 g, 3.47 mmol), triethylamine (1.10 g, 1.51 n L, 10.87 mmol), and dichloromethane (40 niL) followed by the dropwise addition of Cbz-Cl (0.663 g, 0.552 mL, 3.89 mmol) with stirring. The resulting solution was stirred overnight at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford benzyl 4-(4-(2-((teri-butoxycarbonyl)amino)ethyl)-3- cyclopropylphenyl)piperazine-l-carboxylaie as a colorless oil (1 g, 60%). LCMS (ESI, m/z): 480 [ M ] f | .
Example 38: Intermediate 38. Benzyl 4-(6-(2-((ii?ri-butoxycarbonyl)amino)ethyl)-[l,l'- biphenyl]-3-yl)piperazine-l-carboxylate
Figure imgf000149_0001
intermediate 38
Step 1. terf-Butyl (2-(5-nitro-[l,l'-biphenyI]-2-yl)ethyl)carbamate
[00374] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-butyl N-[2-(2-brotno-4-nitrophenyi)et yl]carbaraate (3.44 g, 9.97 mmol), phenylboronic acid (2.44 g, 20.0 mmol), Pd(dppf)Cl? (0.732 g, 1.00 mmol), CS2CO3 (9,78 g, 30.0 mmol), toluene (20 mL), and water (2 mL). The reaction mixture was stirred
148
144628010 vl overnight at 100 °C and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford tert-butyl (2-(5-nitro- [ 1 , Γ-bi phenyl ]-2-yl)ethy l)carbamate as an orange oil (3.3 g, 97%). LCMS (ESI, m/z): 343 [M+H]+
Step 2, terf-Butyl (2-(5-amino-[l,l'-biphenyI]-2-yl)ethyI)carbamate
[00375] Into a 250-mL round-bottom flask, purged and maintained with nitrogen, was added tert-bu l (2-(5-nitro-[l ,l'-biphenyl]-2-yl)ethyl)carbamate (1.73 g, 5.05 mmol), 10% palladium on carbon (173 mg), and methanol (80 mL). The resulting mixture was sparged with hydrogen and the reaction mixture was stirred for 2 h at RT under a hydrogen atmosphere using a hydrogen balloon. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford tert-butyl (2-(5-amino-[l,r- biphenyl]-2-yl)ethyi)carbamate as a brown oil (1.5 g, 95%). LCMS (EST m/z): 313 M i Ij .
Step 3. tert-Beiyl (2-(5-(piperazin-l-yl)-[l,l'-biphenyI]-2-y!)ethyl)carbamate
[00376] Into a 250-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-butyl (2-(5-amino-[l, l'-biphenyl]-2-yl)ethyl)carbamate (1.50 g, 4.80 mmol), bis(2-chloroethyl)amine hydrochloride (1.30 g, 7.28 mmol), potassium carbonate (2.00 g, 14.5 mmol), and BuOH (30 mL). The resulting solution was stirred overnight at 100 °C in an oil bath and then the resulting mixture was concentrated in vacuo to afford crude tert-butyl (2~(5- (piperazin-l-yl)-[l,r-biphenyl]-2-yl)ethyl)carbamate as a brown solid (1.7 g) that was carried on without further purification. LCMS (ESI, m/z): 382 [M+fff .
Step 4, Benzyl 4-(6-(2-((teri-butoxycarbonyl)amino)ethyl)-[l, -biphenyl]-3-yl)piperazine-l- car boxy! ate
[00377] Into a 250-mL round-bottom flask was added tert-butyl (2-(5-(piperaziri- 1 -yl)-[ 1, '- biphenyl]-2-yl)ethyl)carbamate (1.70 g, 4.46 mmol), dichloromethane (100 mL), and TEA (1.40 g, 13.8 mmol). This was followed by the dropwise addition of Cbz-Cl (1.20 g, 1.00 mL, 7.03 mmol) with stirring. The resulting solution was stilted for 2 h at RT and then was concentrated in vacuo to afford the crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford benzyl 4-(6-(2-((tert-butoxycarbonyl)amino)
149
144628010 vl ethyl)-[l,r-biphenyl]-3-yl)piperazine-l-carboxylate as a brown solid (800 mg, 35%). LCMS (ES!. zy 516 [M-H]
Example 39: Intermediate 39. Benzyl 4-(4-bromo-2-ethoxyphenyl)piperazine-l- carboxylate
Figure imgf000151_0001
Intermediate 39
Step L l-(2-Ethoxyphenyl)piperazine
[00378] Into a 250-niL round-bottom flask was added 2-ethoxyaniline (4.11 g, 30.0 mmol), bis(2-chloroethyI)amine hydrochloride (6.40 g, 35.9 mmol), potassium carbonate (12.4 g, 89.9 mmol), and DMSO (30 mL). The reaction mixture was stirred overnight at 140 °C and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with dichloromethane/methanol (5: 1) to afford l -(2-ethoxyphenyl)piperazine as a brown oil (5.1 g, 83%). LCMS (ESI, m/z): 207 [M+H]+.
Step 2, Benzyl 4-(2-ethoxyphenyl)piperazine-l-carboxylate
[00379] Into a 500-mL round-bottom flask was added 1 -(2-ethoxyphenyl)piperazine (9.20 g, 44.6 mmol), triethylamine (14.2 g, 140 mmol), and dichloromethane (250 mL). Benzyl chioroformate (9.60 g, 8.00 mL, 56.3 mmol) was added and the resulting solution was stirred for 1 h at RT. The reaction was then concentrated in vacuo and the caide product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford benzyl 4-(2- ethoxyphenyl)piperazine-l-carboxylate as a purple solid (3.1 g, 20%). LCMS (ESI, m/z): 341 [ M i l ! .
150
144628010 vl Step 3. Benzyl 4-(4-bromo-2-ethoxyphenyl)piperazine-l-carboxylate
[00380] Into a 50-mL round-bottom flask was added benzyl 4-(2-ethoxyphenyl)piperazine-l- carboxylate (0.680 g, 2.00 mmol), NaOAc (0, 164 g, 2.00 mmol), AcOH (14 mL), and water (3 mL). To the resulting solution Br?. (0.316 g, 0.102 mL, 1.98 mmol) was added dropwise at 0 °C. The reaction mixture was stirred for 2 h at 0 °C and then concentrated in vacuo and diluted with water (20 mL). The pH of the solution was adjusted to approximately 7 with aqueous sodium hydroxide (1 M). The resulting solution was extracted with ethyl acetate (3 x 50-mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford benzyl 4-(4-bromo-2-ethoxyphenyl)piperazine-l-carboxylate as a purple oil (280 rag, 33%). I .CMS (ESI, m/z); 419 ( i l l .
Example 40: intermediate 40. Methyl 5-bromo-2,3--d5hydro-lli-i8idene-2-carboxyIate
Figure imgf000152_0001
intermediate 40
Step J, Methyl 5-bromo-l-oxo-2,3-dihydro-lH-indene-2-carboxyIate
[00381] Into a 250-mL round-bottom flask was added 5-bromo-2,3-dihydro-lH-inden-l-one
(6,93 g, 32,8 mraol) and THF (30 mL). To the resulting solution was added sodium hydride (60% dispersion in mineral oil; 2.64 g, 110 mmol), followed by dimethyl carbonate (4.49 g, 4.2 rnL, 49.9 mmol ). The reaction mixture was stirred for 30 min at 65 °C. The reaction was cooled to RT, and was quenched with HC1 (3 M) to a final pH of approximately 6. The resulting solution was extracted with ethyl acetate (3 x 100 mL). The combined organic layers were then dried over anhydrous magnesium sulfate, filtered and concentrated in vacuo to afford methyl 5- bromo-l-oxo-2,3-dihydro-I -indene-2-carboxylate as a yellow solid (8.5 g, 96%). LCMS (ESI, m/z): 269 | M ! 1 | .
Step 2. Methyl 5-bromo-2,3-dihydro-l/i-indene-2-carboxylate
[00382] Into a 250-mL round-bottom flask was added methyl 5-bromo-l-oxo-2,3-dihydro-lH- indene-2-carboxylate (7.80 g, 29,0 mmol) and trifiuoroacetic acid (132 mL). Triethylsilane (20.4 g, 28 mL, 175 mmol) was added and the resulting solution was stirred for 2 h at RT. The reaction
151
144628010 vl mixture was concentrated in vacuo and diluted with water (100 mL). The solution was then extracted with ethyl acetate (3 x 600 mL), The combined organic layers were then dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo to afford methyl 5-bromo-2,3- dihydro-lH-indene-2-carboxylate as a yellow solid (6.21 g, 84%). LCMS (ESI, m/z): 255 \\ - \ \ \
Example 41: intermediate 41. Methyl 4-(2-((teri-b5itoxycarbonyi)aiiiino)eihyI)benzoaie
CO (10 aim)
Figure imgf000153_0001
MeOH, 100 °C
Step
[00383] Into a 100-mL pressure tank reactor was added fe/Y-butyl N-[2-(4- bromophenyl)ethyl]carbamate (1.50 g, 5.00 mmol), Pd(dppf)Ch (0.367 g, 0.500 mrnol), triethylamine (l .Olg, 1.39 mL, 9.98 mmol), and methanol (20 mL). The reaction vessel was pressurized with CO (10 atm) and the reaction mixture was stirred overnight at 100 °C under carbon raonoxide atmosphere (10 atm). The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to provide a crude product that was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford methyl 4-(2-((¾r?-buioxycarbonyl)amino)ethyl)benzoaie as a white solid (1.1 g, 79%). LCMS
): 280 | \i Π ]
Example 42: Intermediate 42. Methyl 3-(2-aminoethyl)benzoate
Figure imgf000153_0002
[00384] Into a 250-mL round-bottom flask purged and maintained with nitrogen was added methyl 3-(cyanomethyl)benzoate (1.00 g, 5.71 mmol), methanol (60 mL), and aqueous HCI (1 M; 0.66 mL). This was followed by the addition of 10% palladium on carbon (0.48 g). The resulting mixture was sparged with hydrogen and then stirred for 2 h at RT under a hydrogen atmosphere using a hydrogen balloon. The reaction was vented to nitrogen and the solids were
152
144628010 vl removed by filtration over Celite. The filtrate was concentrated in vacuo to afford methyl 3-(2- aminoethyl)benzoate as a yellow solid (0.8 g, 78%), LCMS (EST m/zX 180 [M+H]÷.
[00385] The Intermediates in Table 2 below were synthesized according to Steps 5 and 6 of the procedure outlined above for Example 28: Intermediate 28, using the appropriate synthetic precursors. Either Method A (Steps 1 and 2 below) or Method B (Step 2 only) was used to obtain the title compounds below.
Figure imgf000154_0001
fable 2:
Figure imgf000154_0002
153
144628010 vl
Figure imgf000155_0001
154
144628010 vl Example 44: Intermediate 44. tert-B tyl 4-(4-(2-aminoethyl)-2-fluorophenyl)piperazine-I- carboxylate
Figure imgf000156_0001
Step 1. Benzyl 4-(4-bromo-2-fluorophenyl)piperazine~l-carboxylate
[00386] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added 4-bromo-2-fluoro-l-iodobenzene (3.00 g, 10.0 mmol), benzyl piperazine- 1-carboxylate (2.60 g, 11.8 mmol), Pd2(dba)3 (0.458 g, 0,500 mmol), XantPhos (0.595 g, 1.00 mmol), and NaOtBu (2.88 g, 30.0 mmol). Toluene (30 mL) was added and the reaction mixture was stirred for 2 h at 80 °C then concentrated in vacuo to a crude material that was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford benzyl 4-(4-bromo-2- fluorophenyl)piperazine-l-carboxylate as a brown oil (2.5 g, 64%). LCMS (ESI, m z): 393[ \i i l l .
Step 2. l-(4-Bromo-2-fluorophenyl)piperazine
[00387] Into a 100-mL round-bottom flask was added a solution of benzyl 4-(4-bromo-2- fluorophenyl)piperazine-l-carboxylate (3.00 g, 7.63 mmol) in dichloromethane (20 mL) followed by boron tribromide (3.00 mL, 22.8 mmol) dropwise at -20 °C. The resulting solution was siined for 1 h at -20 °C and then quenched with methanol (10 mL). The reaction mixture was concentrated in vacuo to afford a cmde product that was purified by FCC eluting with ethyl
155
144628010 vl acetate/petroleum ether (1 :3) to afford l-(4-bromo-2-fluorophenyi)piperazine as a yellow solid (1.6 g, 81%). LCMS (ESI, m/z): 259 j'M -H j .
Step 3. 4-(4-bromo-2-fluorophenyl)piperazine-l-carboxylate
[00388] Into a 50-mL round-bottom flask was added l-(4-bromo-2-fluorophenyl)piperazine (1.5 g, 6.0 mmol), 4-dimethylaminopyridine (0.150 g, 1.0 mmol), triethylamine (1.76 g, 2.42 mL, 17.5 mmol), di-fert-butyl di carbonate (1 ,52 g, 7,50 mmol), and dichloromethane (20 mL), The resulting solution was stirred for 2 h at 40 °C in an oil bath and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford tert- vXy\ 4-(4-bromo-2-fluorophenyl)piperazine-l-carboxylate as a white solid (2,02 g, 98%). LCMS (ESI, m/z): 359 | M ! ! l .
Step 4, tert~Buty\ 4-(4-(2-(((benzyIoxy)carbonyl)amino)ethyl)-2-fluorophenyI)piperazine-l- carboxyiate
[00389] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added fe/ -butyl 4-(4-biOmo-2-fluorophenyl)piperazine-l-carboxylate (0.500 g, 1.39 mmol), potassium (2-(benzyloxycarbonylaraino)ethyl } trifluoroborate (0.398 g, 1.40 mmol; either purchased commercially or prepared according to the procedure of Molander, G. A. et al; J. Org. Chem. 2007, 72, 8422.), Pd(dppf)Ci2 (0.114 g, 0.160 mmol), CsiiCCb (1.36 g, 4.18 mmol), RuPhos (0.130 g, 0.280 mmol), and toluene/water (10 mL/ 3 mL). The resulting mixture was stirred for 5 h at 95 °C in an oil bath and then cooled to RT, and concentrated in vacuo. The resulting cmde product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford ter /-butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2-fluorophenyl) piperazine-1 - carboxylate as a brown oil (400 mg, 62.8%). LCMS (ESI, m/z): 458 [M+H .
Step 5. ferf-Butyl 4-(4-(2-aminoethyl)-2-fIuorophenyl)piperazine-l-carboxylate
[00390] into a 50-mL round-bottom flask purged with nitrogen was added fer /-butyl 4-(4~(2~ (((benzyloxy)carbonyl)amino)ethyl)-2-fluorophenyl)piperazine-l -carboxylate (0.30 g, 0.66 mmol) and 10% palladium on carbon (30 mg). Methanol (10 mL) was added under an atmophere of nitrogen and the solution was then purged with a hydrogen filled balloon. The resulting suspension was stirred for 1 h under an atmosphere of hydrogen. The reaction was vented to nitrogen and the solids were then removed by filtration over Celite. The filtrate was
156
144628010 vl concentrated in vacuo to afford fert-butyl 4-(4-(2-aminoethyl)-2-fluoropheny azme- carboxylate as a white solid (200 rng, 94%), LCMS (ESI, m/z): 324 [M÷H]+,
Example 45 Intermediate 45. fert-Butyl 4~{4~(2~ l)pi
Figure imgf000158_0001
Step 1, Benzyl (4-bromophenethyl)carbamate
[00391] To a mixture of 2-(4-bromophenyl)ethanamine (10.0 g, 50.0 mmol) and saturated aqueous sodium bicarbonate (375 ml) was slowly added Cbz-Cl (10.3 g, 8.56 ml, 60.0 mmol). The reaction mixture was allowed to stir at RT for 1 hour (a white precipitate forms) and then extracted with EtOAc (2 x 200 mL). The organic layers were combined, washed with water (100 mL) and brine (100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with 5 - 20% EtOAc in hexanes to afford benzyl (4-bromophenethyi)earbamate as a white solid (16.7g, 100%). LCMS (ESI, m/z): 334, 336 [M ] [J .
Step 2. 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-3,6-dihydropyridine- l(2H)-carboxylate
[00392] Into a 50-mL microwave tube was added benzyl (4-bromophenethyl)carbamate (1.45 g, 4.34 mmol), fert-hutyl 4-(tetranietliyl-l,3,2-dioxaborolan-2-yl)-l,2,3,6-tetrahydropyridine-l- carboxylate (2.69 g, 8.70 mmol), Pd(dppf)Ch (0.32 g, 0.44 mmol), potassium carbonate (1.20 g, 8.68 mmol), ethano! (10 mL) and water (2 mL), The mixture was heated under microwave irradiation for 1 h at 130 °C. The solids were removed by filtration and the filtrate was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 10) to afford tert-butyl 4-(4-(2- (((benzyl ox )carbonyl)arnino)ethyl)phenyl)-3 ,6-dihydropyridine- 1 (2H)-carboxyl ate as a yellow solid (218 mg, 12%). LCMS (ESI, m/z): 437 [M+Hf.
157
144628010 vl Step 3. iert~Buty\ 4-(4-(2-aminoethyl)phenyI)piperidine-l-carboxyIate
[00393] Into a 100-mL round-bottom flask purged with nitrogen was added fert-butyl 4-(4-(2- {((benzyloxy)carbonyl)am!no)eihyi)phenyl)-3,6~dihydropyiidi (0,200 g,
0.46 mmol), 10% palladium on carbon (100 mg), and methanol (10 mL). The resulting mixture was sparged with hydrogen and stirred overnight at RT under a hydrogen atmosphere. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo. The resulting crude product was purified by FCC eiuting with dichloromethane/methanol (3: 1) to afford tert-buiyl 4-(4-(2-aminoethyl)phenyl)piperidine-l- carboxylate as a brown oil (400 mg). LCMS (ESI, m/z): 305 | M ! ! j .
Example 46: Intermediate 46. tert-Butyl 4-(4~ ,1.
Figure imgf000159_0001
Step 1 tert- utyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)piperazine-l-
[00394] Into a 1-L round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added benzyl (4-bromophenethyl)carbamate (50,0 g, 150 mmol) and anhydrous toluene (500 mL). To the resulting solution was added tert-hutyl piperazine-l-carboxylate (34.0 g, 183 mmol), Pd(OAc)2 (3.40 g, 15,1 mmol), XPhos (14.3 g, 30,0 mmol), and CS2CO3 (98.0 g, 301 mmol). The reaction mixture was stirred overnight at 105 °C in an oil bath and then cooled to RT, and quenched with H2O (500 mL). The mixture was extracted with ethyl acetate (2 x 500-mL), the combined organic layers were washed with brine (1 x 500 mL). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eiuting with ethyl acetate/petroleum ether (1 : 1) to afford tert- butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyi)piperazine-l-carboxylate as a yellow solid (26 g, 40%). LCMS (ESI, m/z): 440 [M+H]+.
158
144628010 vl Step 2. tert-But l 4-(4-(2-aminoethyl)phenyI)piperazine-l-carboxylate
[00395] Into a 500-mL round-bottom flask that was purged and maintained under an atmophere of nitrogen was added tert-butyl 4-(4-(2-
(((benzyloxy)carbonyl)amino)ethyl)phenyl)piperazine-l-carboxylate (26.0 g, 59.2 mmol), 10% palladium on carbon (13.0 g), and methanol (300 mL). The resulting mixture was sparged with a hydrogen filled balloon and the reaction was stirred at RT under hydrogen for 1 h. The reaction was vented to nitrogen, the solids were removed by filtration over C elite and the filtrate was concentrated in vacuo to afford fen-butyl 4-(4-(2-aminoethyl)phenyl)piperazine-l-carboxylate as a yellow solid (18.0 g, 100%). i CMS (ESI, m/z) 306 [M+H]+.
Example 47: Intermediate 47. ferf-Butyl 4-(4-(2-aminoethyI)-2-chlorophenyl)piperazine-l-
Figure imgf000160_0001
Itstermediais 47
Step 1. teri-Butyl 4-(4-(2-(((benzjrloxy)carbonyl)amino)ethyl)-2-chlorophenyl)piperazine-l- carboxylate
[00396] Into a 500-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added fert-butyl 4-[4-(2-[[(benzyloxy)carbonyl]amino]ethyl)phenyl]piperazine- 1-carboxylate (7,00 g, 15.9 mmol), NCS (2.13 g, 16.0 rnmol), and dichloromethane (120 mL). The reaction mixture was stirred overnight at reflux and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (2:3) to afford tert- ut l 4-(4-(2-(((benzyloxy)carbonyi)amino)ethyl)-2-chlorophenyl)piperazine-l - carboxylate as a yellow oil (8.0 g). The material was used without further purification. LCMS (ESI, m/z): 374 I'M- H] '
Step 2, terf-Butyl 4-(4-(2-aminoethyl)-2-chlorophenyl)piperazine-l-carboxylate
[00397] Into a 250-mL round-bottom flask, purged and maintained with nitrogen, was added ifiTjf-butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2-chlorophenyl)piperazine-l-carboxylate
(4.00 g, 8.44 mmol), Raney Nickel (1.0 g), and methanol (150 mL). The reaction mixture was
159
144628010 vl sparged with a hydrogen filled balloon and stirred for 3 days at RT under hydrogen. The reaction was vented to nitrogen and the solids were removed by filtration over C elite. The filtrate was concentrated in vacuo to afford fert-butyl 4-(4-(2-aminoethyl)-2- chlorophenyl)piperazine-l-carboxylate as a gray solid (3.0 g). The material was used without further purification. LCMS (ESI, m'z): 340 [M+H]+.
Example 48-1: Intermediate 48-1. te/ΐ- Butyl (i?)-(l-(4-(2-aminoethyl)-2-chlorophenyl) pvrri
Figure imgf000161_0001
intermediate 48-1
Step I. terf-Butyl (/?)-(l-(4-(2-aininoethyl)phenyl)pyrroIidin-3-yl)(methyl)carbamate
[00398] Into a 250-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added benzyl N-[2-(4-bromophenyl)ethyl]carbamate (2.00 g, 5.98 mmol), tert- butyl jV-methyl-A-[(3R)-pyrrolidin-3-yl]carbamate (1.30 g, 6,49 mmol), toluene (20 mL), Pd(OAc)2 (0.135 g, 0.600 mmol), XantPhos (0.347 g, 0.600 mmol), and NaOtBu (1.70 g, 17.69 mmol). The reaction mixture was stirred overnight at 100 °C in an oil bath and then cooled and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with dichloromethane/methanoi (10: 1) to afford feT/-butyl (R)-(l-(4-(2- aminoethyl)phenyi)pyrrolidin-3-yl)(methyl)carbamate as yellow oil ( 1.0 g, 52 %). LCMS (ESI, m/z): 320 ! M ! l | .
Step 2, iert-Butyl (i?)-(l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyI)pyrrolidin-3- yl)(methyl)carbamate
[00399] Into a 250-mL round-bottom flask was added ferf-butyl (R)-(l-(4-(2- aminoethyl)phenyl)pyrrolidin-3-yl)(methyl)carbamate (1.50 g, 4.70 mmol). dichlorornethane (20
160
144628010 vl mL), and triethylamine (1.50 g, 2.07 mL, 14.8 mmol). The reaction mixture was cooled to 0 °C and then benzyl chloroformate (0,941 g, 0.784 mL, 5.52 mmol) was added dropwise, The resulting solution was stirred for 1 h at RT and then quenched with water (50-mL). The resulting solution was extracted with dichlorom ethane (3 x 20 mL), and the organic layers were combined. The combined organic layers were washed with brine (2 x 80 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford ter t-buty\ (R)-(l-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)phenyl)pyrrolidin-3-yl)(methyl)carbamate as a yellow oil (1.5 g, 70%). I MS (ESI, m/z): 454 j M ϊ I ] .
Step 3. feri-Buty! ( i)-(l-(4-(2-(((benzyloxy)carbonyl)aiiiino)ethyl)-2-chlorophenyl) pyrrolidin-3-yl)(methyl)carbamate
[00400] Into a 100-mL round-bottom flask was added tert-butyl (R)-(l-(4-(2- (((benz}doxy)carbonyl)amino)ethyl)phenyl)pyn'olidin-3-yl)(methyl)carbamate (0.600 g, 1.32 mmol), NCS (0.177 g, 1.33 mmol), and dichloromethane (10 mL). The resulting solution was stirred for 2 h at 50 °C in an oil bath and then cooled and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford terr-butyl (R)-(l-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)-2-chlorophenyl)pyrrolidin-3-yl)(methyl)carbamate as yellow oil (300 mg, 46%). LCMS (ES, m/z): 488 [M+H .
Step 4. tert-Bnt l (/?)-(l-(4-(2-aminoethyl)-2-chlorophenyl)pyrrolidin-3- yl)(methyl)carbamate
[0040 J] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-hutyl (R)-(l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2- chlorophenyl.) pyrrolidin-3-yl)(methyl)carbamate (0,300 g, 0,610 mmol), methanol (20 mL), and Raney Nickel (50 mg). The reaction mixture was stirred overnight under hydrogen (balloon pressure) at RT and then the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford fert-butyl (R)-(l-(4-(2-aminoethyl)-2-chloiOphenyl)pyrrolidin-3- yl)(methyl)carbamate as yellow oil (170 mg, 78%). LCMS (ESI, m/z) 354 [M+H ,
[00402] The Intermediates in Table 3 below were synthesized according to the procedures outlined above for Example 48-1, Intermediate 48-1, using the appropriate synthetic precursors.
161
144628010 vl Table 3:
Figure imgf000163_0002
)Ie 49: Intermediate 49. ieri-Butyl 4-(4-(2-aminoethyl)-2,5-difluorophenyl) piperazine-l-carboxylate
Figure imgf000163_0001
Step J. ieri-Butyl 4-(4-bromo-2,5-difluorophenyl)piperazine-l-carboxylate
[00403] Into a 500-ml round-bottom flask was added l,4-dibromo-2,5-difluorobenzene ( 10.0 g, 36.0 mmol), tert-butyl piperazine-l-carboxylate (7.27 g, 37.8 mmol), XantPhos (2.09 g, 3.60 mmol), and Pd-(clba); (1.65 g, 1 .80 mmol). Toluene (100 mL) was added, and the resulting mixture was sparged with nitrogen for 10 min. NaOtBu (10.4 g, 108 mmol) was added and the nitrogen sparging was continued for an additional 5 min. The reaction mixture was heated under an atmophere of nitrogen at 80 °C in an oil bath for 15 min. The reaction was cooled to RT, and filtered over Celite. The filtrate was washed with water (2 x 100 ml.) and brine (1 x 100 mL). The aqueous layers were combined and extracted with EtOAc (200 mL). The combined organic layers were dried over MgSCn, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/hexanes (1 :5) to afford fert-butyl 4-(4-bromo-2,5-
162
144628010 vl difluorophenyl)piperazine-l-carboxylate as a green solid (8.12 g). LCMS (ESI, m/z): 378 \ \ I i f ! .
Step 2. torf-Butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl) piperazine-l-carboxylate
[00404] Into a 500-mL round-bottom flask was added tot-butyl 4-(4-bromo-2,5- difluorophenyl) piperazine-l-carboxylate (8.13 g, 19.4 mmol), potassium (2- (benzyloxycarbonylamino)eihyi) trifluoroborate (8.30 g, 29.1 mmol), Pd(dppf)Ch (3.48 g, 4.27 mmol), RuPhos (3.81 g, 7.76 mmol), cesium carbonate (19.0 g, 58,2 mmol), toluene (120 mL), and water (40 mL). The resulting mixture was sparged with nitrogen for 20 min. The reaction mixture heated at 95 °C in an oil bath for 3 hours. The reaction was cooled to RT and then extracted with EtOAc (3 x 200 mL). The combined organic layers were washed with water (200 mL) and brine (200 mL). dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified by FCC eluting with EtOAc/Hexanes (1 :4) to afford teri-butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl)piperazine- 1 - carboxylate as a beige solid (5.39 g, 58%). LCMS (ESI, m/z): 416 | \! l ! j
Step 3. ferf-Butyl 4-(4-(2-aminoethyl)-2,S-difluorophenyl)piperazine-l-carboxylate
[00405] Into a 500-mL round-bottom flask that was purged and maintained under an atmophere of nitrogen was added fer /-butyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2,5- difluorophenyl)piperazine-l-carboxylate (5.47 g, 11.5 mmol), 10% palladium on carbon (1.22 g) and MeOH (110 mL). The reaction flask was flushed with nitrogen, followed by hydrogen from a balloon (3x). The reaction mixture was stirred under hydrogen for 1.5 h and then vented with nitrogen and filtered over Celite. The resulting filtrate was concentrated m vacuo by co- evaporating with DCM and EtOAc to afford fen-butyl 4-(4-(2-aminoethyl)-2,5- difluorophenyl)piperazine-l-carboxylate as a light yellow gum (3.9 g, 99%) that was used without further purification. LCMS (ESI, m/z): 342 j M i l l".
[00406] The Intermediates in Table 4 below were synthesized according to Steps 2 and 3 of the procedure outlined above for Example 49, Intermediate 49, using the appropriate synthetic precursors.
Table 4:
163
144628010 vl
Figure imgf000165_0001
carboxylate Example 51 : Intermediate 5L tert- utyl (l-(4-(2-aminoethyl)-2,5-difluorophenyl)azetidin-3-
Figure imgf000166_0001
Sie 3 intermediate 51
1. iertA\n\x\ (l-(4-bromo-2,5-difluorophenyl)azetidin-3-yI)(methyl)carbamate
[00407] Into a 100-mL round-bottom flask was added fert-butyi N-[l -(4-bromo-2,5- difluorophenyl)azetidin-3-yl]carbamate (2.00 g, 5.51 mmoi), and DMF (50 mL). This was followed by the addition of sodium hydride in mineral oil (60%, 0.330 g, 8.25 mmol) in portions at 0 °C. The resulting solution was stirred for 30 min at 0 °C and then iodomethane (0.860 g, 0.377 mL, 6.06 mmol) was added dropwise with stirring. The resulting solution was stirred for another 1 h at 0 °C and then quenched with water ( 100 mL). The resulting solution was extracted with ethyl acetate (3 x 100 mL) and the combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford fert-butyl (l-(4-brorao- 2,5-difluorophenyl)azetidin-3-yl)(methyl)carbamate as a light yellow solid (1.8 g, 87%). LCMS (ESI, rn/z) 377, 379 [M+H]+.
Step 2. (l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl)azetidin- 3-yl)(methyl)carbamate
[00408] Into a 100-mL 3 -necked round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added fert-butyl (l -(4-bromo-2,5-difluorophenyl)azetidin-3- yl)(methyl)carbamate (1.00 g, 2.65 mmol), CS2CO3 (2.59 g, 7.95 mmol), potassium (2- (benzyioxyca.tboriylati]itto)e hyl) trifluoroborate (0.907 g, 3.18 mmol), Pd(dppf)C (0.194 g, 0.270 mmol), RuPhos (0.25 g, 0.53 mmol), toluene (30 mL), and water (10 mL). The resulting solution was stirred for 3 h at 95 °C and then cooled to RT. The resulting mixture was poured into water (20 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude
165
144628010 vl product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (PE/EA ::= 100: 1 to 5: 1) to afford /er/-butyl (l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)- 2,5-difluorophenyl)azetidin-3-yl)(methyl)carbamate as a yellow oil (650 nig, 52%), LCMS (ESI, m/z): 476 I M - f l j .
Step 3, tert-Butyl (l-(4-(2-aminoethyl)-2,5-difluorophenyI)azetidin-3-yl)(methyl)carbamate
[00409] Into a 50-mL 3-necked round-bottom flask, purged and maintained with nitrogen, was added teri-butyl (l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl)azetidin-3- yl)(methyl)carbamate (0.30 g, 0.63 mmol) and methanol (15 mL). This was followed by the addition of 10% palladium on carbon (300 mg). The reaction mixture was sparged with hydrogen and then stirred for 2 h at 25 °C under a hydrogen atmosphere. The solids were removed by filtration over Celite, and the filtrate was concentrated in vacuo to afford teri-butyl (l-(4-(2-aminoethyl)-2,5-difluorophenyl)azetidin-3-yl)(methyI)carbamate as light yellow oil (180 mg, 84%), LCMS (ESI, m/z): 342 j\l
Example 52: Intermediate 52. tert-Butyl 4-(7-bromo-2,3-dihydro-lJfiT-inden-4- yOpiperazine-l-carboxylate
Figure imgf000167_0001
Step 1. iV-(2,3-dihydro-l -r-iiiden-4-yl)acetamide
[00410] Into a 250-mL round-bottom flask was added 2,3-dihydro-l -inden-4-amine (5.00 g, 37.5 mmol) and ethanol (100 mL). Acetic anhydride (7.67 g, 7.10 mL, 75, 1 mmol) was added and the resulting solution was stirred for 1 h at RT. The reaction mixture was concentrated in vacuo and then diluted with ether (20 mL). The solid product was collected by filtration and dried in vacuo to afford A -(2,3-dihydro-lH-inden-4-yl)acetamide as an off-white solid (6.0 g, 91%). LCMS (ESI, m/z): i 76 [ M 1 1 i
166
144628010 vl Step 2. N-(7-bromo-2,3-dihydro-lH-inden-4-yl)acetamide
[00411] Into a 250-mL round-bottom flask was added N-(2,3-dihydro-lH-inden-4- yljacetamide (5.00 g, 28.5 mmol) and acetic acid (80 mL). The resulting solution was cooled to 0 °C and then a solution of bromine (9.03 g, 56.5 mmoi) in acetic acid (20 mL) was added dropwise with stirring over 10 rain. The cooling bath was removed and the reaction mixture was stirred for Ih at RT. Water was added and the resulting solid product precipitate was collected by filtration and dried in vacuo to afford N"-(7-bromo-2,3-dihydro-lH-inden-4-yl)acetamide as a white solid (7.1 g, 98%). LCMS (ESI, m/z); 254 } M I I I
Step 3, 7-Bromo-2,3-dihydro-lH-inden-4-amine
[00412] Into a 250-mL round-bottom flask was added N-(7-bromo-2,3-dihydro-lH"-inden-4- yl)acetamide (7.00 g, 27.6 mmol) and 6 N HCI (100 mL). The resulting solution was stirred for 4 h at 100 °C and then cooled to RT and the pH was adjusted to approximately 7 with saturated aqueous sodium bicarbonate (caution: gas evolution). The resulting solid product was collected by filtration and dried in vacuo to afford 7-bromo-2,3-dihydro-lH-inden-4-amine as an off-white solid (5.7 g, 98%). LCMS (ESI, m/z): 212 [ M I I ] ' .
Step 4, l-(7-Bromo-2,3-dihydro-lJE -inden-4-yl)piperazine
[00413] Into a 250-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 7-bromo-2,3-dihydro-I /-inden-4-amine (5.70 g, 26.9 mmol), potassium carbonate (15.0 g, 109 mmol), bis(2-chloroethyl)amine hydrochloride (5.74 g, 32.2 mmol), and «-BuOH (80 mL). The reaction mixture was stirred overnight at 100 °C and then concentrated //.' vacuo. The resulting crude product was purified by FCC eluting with dichloromethane/methanol (20: 1) to afford l-(7-bromo-2,3-dihydro-lH-inden-4-yl)piperazine as a yellow solid (3.2 g, 42%). LCMS (ESI, m/z): 281 | M ! ! l .
Step 5. 4-(7-bromo-2,3-dihydro-l/ -inden-4-yl)piperazine-l-carboxylate
[00414] Into a 100-mL round-bottom flask was added l-(7-bromo-2,3-dihydro-lH-inden-4- yOpiperazine (1.00 g, 3.56 mmol), triethylamine (1.08 g, 10.7 mmol), and dichloromethane (15 mL). Di-ferf-butyl di carbonate (1 .17 g, 5.36 mmol) was added and the resulting solution was stirred for lh at 40 °C. The reaction mixture was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :20) to afford tert-
167
144628010 vl butyl 4-(7-bromo-2,3-dihydro-lH-inden-4-yl)piperazine-l-carboxylate as a yellow solid (1.05 g, 77%). LCMS (ESI, m/z) 381 jAj- H ;
Example 53: Intermediate 53. iert-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl) phenyl)-3,8-diazabicyclo [3.2.1] octane-8-carboxylate
Figure imgf000169_0001
CS2CO3
Intermediate !3
Toluene, 100 °C
[00415] Into a 50-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added benzyl 7Y-[2-(4-bromophenyl)ethyl]carbamate (3.15 g, 9.43 mmol), tert- butyl 3,8-diazabicyclo[3.2.1 ]octatie-8-carboxylate (2.00 g, 9.43 mmol), Pd2(dba (0,800 g, 1.09 mmol), RuPhos (0.800 g, 1.71 mmol), CS2CO3 (9.00 g, 27.6 mmol) and toluene (50 mL). The reaction mixture was stirred at 100 °C overnight in an oil bath. The resulting mixture was concentrated in vacuo to remove most of the solvent and then diluted with DCM (50 mL). The solids were removed by filtration, the filtrate was concentrated in vacuo, and the crude product that was purified via silica gel chromatography and eiuted with petroleum ether/ethyl acetate (3 : 1) to afford fer -Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl) phenyl)-3,8- diazabicyclo[3.2.1 ]octane-8-carboxylate as a yellow oil (1.2 g, 27%). LCMS (ESI, m/z) 466
[M+H] +.
Example 54-1: Intermediate 54-1. iert-l iiyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-3- lo 13.2.1
Figure imgf000169_0002
Step S
168
144628010 vl Step 1. 2-(4-Bromo-2-fluerophenyl)ethan-i-amine
[00416] Into a 100-mL round-bottom flask was added 2-(4-bromo-2-fluorophenyl)acetonitrile (2.00 g, 9,34 mmol) and tetrahydrofuran (20 mL). lh in THF (1M, 5 mL) was added and the resulting solution was heated and stirred for 6 h at 75 °C and then cooled to RT and quenched with water (50 mL). The resulting solution was extracted with dichloromethane (3 x 30 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford the title compound as a colorless oil (1.4 g, crude). LCMS (ESI, /// r) 2 1 8 [M+H]+.
Step 2, Benzyl (4-brome-2-fluorophenetfayl)carbamate
[00417] Into a 50-niL round-bottom flask was added 2-(4-bromo-2-fluorophenyl)ethan-l- amine (2.00 g, 9.17 mmol), triethylamine (2,33 g, 3.21 mL, 23,0 mmol), and dichloromethane (15 mL). The resulting solution was cooled to 0 °C and benzyl chloroformate (1.60 g, 1.33 mL, 9.38 mmol) was slowly added. The mixture was stirred at this temperature for 17 h. The reaction was quenched with water (30 mL) and then extracted with dichloromethane (3 x 30 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified by FCC eluting with petroleum ether : ethyl acetate (1 : 1) to afford benzyl (4-bromo-2-fluorophenethyl)carbamate as a colorless oil (1.1 g, 34%). LCMS (ESI, m/z) 352 [M+H]+.
Step 3. terf-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-3-fluorophenyl)-3,8- diazabicycIo[3.2.1]octane-8-carboxylate
[00418] Into a 100-mL round-bottom flask was placed benzyl (4-bromo-2- fluorophenethyl)carbamate (0.825 g, 2.34 mmol), tert-butyl 3,8-diazabicyclo[3.2.1]octane-8- carboxylate (0.500 g, 2,36 mmol), Pd(OAc (0.050 g, 0.22 mmol), RuPhos (0.050 g), CsiCC (2.23 g, 6.84 mmol), and toluene (10 mL). The resulting solution was stirred for 17 h at 1.00 °C in an oil bath and then concentrated in vacuo to afford the crude product. To the crude product was added DCM (50-mL) and then the inorganic solids were removed by filtration. The filtrate was concentrated in vacuo to afford the crude product that was purified by FCC eluting with petroleum ether : ethyl acetate (1 : 1) to afford tert-butyl 3-(4~(2-(((benzyloxyjcarbonyl)amino) ethyl)-3-fluorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a yellow oil (15%). LCMS (ESI, m/z): 484 [M+Fl ,
169
144628010 vl [00419] The intermediates in Table 5 below were synthesized according to the procedures outlined above for Example 54-1, Intermediate 54-1, using the appropriate synthetic precursors.
Table 5:
Figure imgf000171_0002
Example 55: S3, Benzyl (4-(4-(2-methoxyetliyl)piperazin-l - yl)phenethyl)carbamate
intermediate 55
Figure imgf000171_0001
Step l H
Step J. Benzyl (4-(piperazin-l-yl)phenethyl)carbamate
[00420] Into a 250-mL 3-necked round-bottom flask, purged and maintained with nitrogen, was added benzyl (4-bromophenethyl)carbamate (4.00 g, 12.0 mmol), ptperazine (1.26 g, 14.6 mmol), cesium carbonate(11.7 g, 35.8 mmol), toluene (80 mL), XPhos ( 1.14 g, 2.39 mmol), and Pd(OAc)2 (0,270 g, 1.20 mmol). The resulting solution was stirred overnight at 100 °C and then cooled to RT. The resulting mixture was concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (2: 1) to afford benzyl (4-(piperazin-l-yl)phenethyl)carbamate as light yellow oil (1.6 g, 39%). LC S (ESI, m/z): 340 [M+Hf .
170
144628010 vl Step 2. Benzyl (4-(4-(2-methoxyethyl)piperazin-l-yl)phenethyl)carbaniate
[00421] Into a 100-mL round-bottom flask was added benzyl (4-(piperazin-l- yl)phenethy!)carbamate (0.600 g, 1.77 mmol), 3 -bromo-2-methoxy ethane (0369 g, 2.65 mmol), potassium carbonate (0.733 g, 5.27 mmol), and DMF (20 mL). The reaction was stirred overnight at 60 °C and then cooled and extracted with ethyl acetate (3 x 100 mL). The combined organic layers were washed with brine (2 x 100 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1 ) to afford benzyl (4-(4-(2-methoxyethyl)piperazin-l- yl)phenethyl)carbamate as a gray oil (280 mg, 40%). LCMS (ESI, m/z): 398 | M I I I .
Example 56: Intermediate 56. Benzyl (4-(4-(oxetan-2-ylmethyl)piperazin-l- yl)phenethyl)carbamate
Figure imgf000172_0001
DMF, 80 °C
Intermediate 55
[00422] Into a 100-mL round-bottom flask was added benzyl (4-(piperazin-l- yl)phenethyl)carbamate (0,570 g, 1.68 mmol) and DMF (20 mL). 2-(Iodomethyl)oxetane (0.500 g, 2.53 mmol) was added followed by potassium carbonate (0.930 g, 6.74 mmol). The resulting suspension was stirred for 4 h at 80 °C and then cooled, and quenched with water (20 mL). The resulting solution was extracted with ethyl acetate (3 15 mL). The combined organic layers were washed with water (3 x 20 mL) and brine (3 x 20 mL). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with dichloromethane/rnethanol (10: 1) to afford benzyl (4-(4-(oxetan-2- ylmethyl)piperazin-l-yl)phenethyl)carbamate as a gray solid (280 mg, 41%). LCMS (ESL nv'z): 410 [ ΧΙ Ι ί Γ
171
144628010 vl JLxami 57: Intermediate 57, feri- Butyl 4-(4-(2-(((benzyIoxy)carbonyI)amino) '£yi-butyldimethylsilyl)oxy)methyl)piperazine-l-carboxylate
Figure imgf000173_0001
dioxarse, 100 °C
Step 1. 2-(((2'£r2'-butyldiniethylsily])oxy)methyl)piperazine-l-carboxylate
[00423] Into a 250-mL round-bottom flask was added fert-butyl 2- (hydroxymethyl)piperazine-l-carboxylate (2.00 g, 9.25 rnmol) and dichlorornethane (30 rnL). Tri ethyl ami e (2.30 g, 3.17 niL, 22.7 raraol) was added followed by tert- butyl(chloro)dimethylsilane (1.40 g, 9.29 mmol). The resulting solution was stirred overnight at RT and then concentrated in vacuo to provide a crude product that was purified by FCC eluting with dichioromethane/methanol (40: 1) to afford tert-bv& \ 2-({{tert~ butyldimethylsilyl)oxy)methyl) piperazine-l-carboxylate as a yellow oil (2.0 g, 65%). LCMS (ESI. /// :·;·. 331 | ~H] .
Step 2. tert- utyl 4-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-2-(((i'ert'- butyIdimethylsilyl)oxy)methyl)piperazine-l-carboxylate
[00424] Into a 250-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added benzyl N"-[2-(4-bromophenyl)ethyl]carbamate (2.00 g, 5.98 mmol), fert-butyl 2-(((iert-butyldimethylsilyl)oxy)methyl)piperazine-l-carboxylate (2.00 g, 6.05 mmol), XPhos (0.286 g), NaOtBu (1.20 g, 12.5 mmol), Pd(OAc)2 (0.135 g, 0.60 mmol), and dioxane (25 ml,). The reaction mixture was stirred overnight at 100 °C and then diluted with water (80 mL) and extracted with ethyl acetate (3 x 20 mL). The combined organic layers were washed with brine (2 x 80 mL). The organic layer was then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford fert-butyl 4-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)phenyl)-2-(((½ri-butyldimethylsilyl)oxy)methyl)piperazine- 1-carboxylate as a yellow oil ( 1.2 g, 34%). LCMS (ESI, m/z): 584 [ M i l ] .
172
144628010 vl Example 58-1: Intermediate 58-1. tert-Butyl 4-(4-bromo-2,3,6-trifluorophenyl)piperazine-
1-earboxylate
Figure imgf000174_0001
Step 1
Step 1, tert-Baiyl 4~(2,3,6~irifliioropi5ei¾yl)piperazis5e~l-carboxylate
[00425] Into a 250-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 2-brofflo-l,3,4-trifluorobenzene (5.00 g, 23.7 mrnol), ferf- butyl piperazine- 1 -carboxyi ate (4.43 g, 23,8 mmol), Pd2(dba)s (1.10 g, 1.20 mmol), BINAP (1.50 g, 2.41 niniol), NaOtBu (6.91 g, 71.9 mmol), and toluene (50 mL). The reaction mixture was stirred for 3 h at 100 °C and then cooled and concentrated in vacuo. The resulting cmde product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford tot-butyl 4-(2,3,6- trifluorophenyl)piperazine-l-carboxylate as a yellow solid (5.3 g, 71%). LCMS (ESI, mJz) 317 [M+H]+.
Step 2. tert-Butyl 4-(4-bromo-2,3,6-trifluorophenyI)piperazine-l-carboxylate
[00426] Into a 100-mL round-bottom flask was added tert-buty\ 4-(2,3,6- trifluorophenyl)piperazine-l-carboxylate (3 ,00 g, 9,48 mmol ), NBS (1.68 g, 9.44 mmol), and dichloromethane (30 mL). The reaction mixture was stirred overnight at reflux and then concentrated in vacuo to provide a crude product that was subject to purification by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford fert-butyl 4-(4-bromo-2,3,6- trifluorophenyl)piperazine-l-carboxylate as a yellow oil (1 ,0 g, 27%), LCMS (ESI, m/z) 395, 397 [M+H]+
[00427] The Intermediate in Table 6 below was synthesized according to the procedures outlined above for Example 58-1, Intermediate 58-1, using the appropriate synthetic precursors.
173
144628010 vl
Figure imgf000175_0001
diazabicvclo[ E-.3.2.11 octane- 8 -carboxvl ate carboxylate
Example 59: Intermediate 59. tert- tyl 4-(4-bromo-3-(methoxymethyl)phenyl)piperazine- l-earboxylate
Figure imgf000175_0002
intermediate S9
Step 1. tert~Buty\ 4-(4-bromo-3-(ethoxycarbonyl)phenyl)piperazine-l-carboxylate
[00428] Into a 100-mL round-bottom flask was added toV-butyl 4~[3- (ethoxycarbonyl)phenyl]piperazine-l-carboxylate (5.00 g, 15.0 mmol) and dichloromethane (50- mL). NBS (3. 18 g, 17.9 mmol) was added and the resulting solution was stirred for 0.5 h at RT. The reaction mixture was concentrated in vacuo and the crude product was purified by FCC eluting with ethyl acetate petroleum ether (1 :3) to afford tot-butyl 4-(4-bromo-3- (ethoxycarbonyl)phenyl)piperazine-l -carboxylate as a light yellow oil (5.8 g, 94%). LCMS (ESI. m/z) 413, 415 j \ i Π Γ.
Step 2, tert-Butyl 4-(<
[00429] Into a 100-mL round-bottom flask was added iert-hutyl 4-(4-bromo-3- (ethoxycarbonyl)phenyl)piperazine-l-carboxylate (3.00 g, 7.26 mmol) and ethanoi (30 niL). This was followed by the portion-wise addition of NaBH4 (1.66 g, 43.9 mmol) at 0 °C. The resulting solution was warmed and stirred overnight at RT and then poured into ice water (200 niL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed
174
144628010 vl with brine (1 x 200 mL) and then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo , The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford tert-buvyl 4-(4-bromo-3-(hydroxymethyl)phenyl)piperazine-l-carboxylate as a light yellow oil (1.77 g, 66%). LCMS (ESI, m/z) 371, 373 [M+H .
Step 3, terf-Butyl 4-(4-bromo-3-(methoxymethyl)phenyl)piperazine-l-carboxylate
[00430] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added a solution of tert-hutyl 4-(4-bromo-3-(hydroxymethyl)phenyl)piperazine- 1-carboxylate (0.650 g, 1.75 mmol) and tetrahydrofuran (10 mL). This was followed by the portion-wise addition of sodium hydride (60% dispersion in mineral oil; 0.141 g, 5.88 mmol) at 0 °C. The resulting solution was warmed and stirred at RT for 1 h. To this was added Mel (0,499 g, 0,218 mL, 3.52 mmol) and the resulting solution was stirred overnight at RT. The reaction was quenched with aqueous saturated NH4C1 (100 mL). The resulting solution was extracted with ethyl acetate (3 x 30 mL) and the organic layers were combined. The combined organic layers were washed with brine (200 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford to^-butyi 4-(4-bromo-3-(methoxymethyl)phenyl)piperazine- 1-carboxylate as a yellow oil (650 mg (crude)). LCMS (ESI, m/z) 385,387 [M+H]+.
Example 60-1: Intermediate 60-1, ierf-Butyl l-(4-bromophenyl)-4,6-dihydropyrrolo[3,4- c]pyrazole-5(li/)-carboxylate
Figure imgf000176_0001
intermediate 60-1
Step 1. tert- ut l (£)-3-((dimethyIamino)methylene)-4-oxopyrrolidine-l-carboxy!ate
[ΘΘ431] Into a 100-mL round-bottom flask was added tert-hutyl 3-oxopyrrolidine-l - carboxylate (10.0 g, 54.0 mmol) and DMF-DMA (50 mL). The resulting solution was stirred for 1 h at 100 °C and then cooled to RT, concentrated in vacuo, and then diluted with ethyl ether (10 mL). The resulting solids were collected by filtration and dried in vacuo to afford tert-butyl (£ - 3-((dimethylamino)methylene)-4-oxopyrrolidine-l-carboxyiate as a yellow solid (7.6 g, 59%). LCMS (ESI, m/z): 241 [M+H]+.
175
144628010 vl Step 2. iert~Buty\ l-(4-bromophenyl)-4,6-dihydropyrrolo[3,4-cjpyrazole-5(lfl)-carboxylate
[00432] Into a 50-mL round-bottom flask, was placed iert-butyl (£)-3- ((dimethylaraino)methylene)-4-oxopyrrolidine-l-caAoxylate (1.0 g, 4.16 mmol), (4- bromophenyl)hydrazine hydrochloride ( 1.03 g, 5.51 mmol), potassium carbonate (1.15 g, 8.32 mmol), and ethanol (10 mL). The resulting solution was stirred for 1 h at 80 °C in an oil bath and then cooled and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with petroleum ether/EtOAc (5: 1) to afford /fert-butyl l-(4- bromophenyl)-4,6-dihydropyrrolo[3,4-c]pyrazole-5(li )-carboxylate as a red oil (0.5 g, 33%). LCMS (ESI, m/z) 364,366 [M+H] +.
[00433] The Intermediate in Table 7 below was synthesized according to the procedures outlined above for Example 60-1, Intermediate 60-1, using the appropriate synthetic precursors.
Table 7:
Figure imgf000177_0001
176
144628010 vl Example 61: Intermediate 61. tert-But l l-(4-(2-(((benzyloxy)carbonyl)amino.
fluorophenyI)-4,6-dihydropyrrolo[3,4-c]pyrazole-5(li/)-carboxylate
Figure imgf000178_0001
intermediate 81
Step 1. ieri-Butyl (4-bromo-3-fluorophenyl)carbamate
[00434] Into a 100-niL round-bottom flask was added 4-bromo-3-fluoroaniline (10.0 g, 52.6 mmol) and dioxane (300 mL). B0C2O (23.1 g, 106 mmol) was added and the resulting solution was stirred overnight at 80 °C. The reaction mixture was cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with petroleum ether/ethyl acetate (5: 1) to afford fenf-butyl (4-bromo-3-fluorophenyl)carbamate as a white solid (16 g, 100%), IH~NMR (300 MHz, }.)\ i SC.W. ) δ pom 9.47 (s, i l l ). 7.64-7.44 (m, 2H), 7.28-7. 10 (m, 2H), 1.47 (s, 9H).
Step 2, (4-(2-(((benzyloxy)carbonyl)amino)ethyl)-3-fluorophenyl)carbamate
[00435] Into a 500-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-buty! (4-bromo-3-f!uorophenyl)carbamate (8.00 g, 27.6 mmol), potassium (2"(benzyloxycarbonyiamino)ethyl) irifluoroboraie (9.47 g, 33.2 mmol), CS2CO3 (27.1 g, 83.2 mmol), PdidppfjCk CH2CI2 (4.52 g, 5.53 mmol), and Ph e/RO (30 ml., 10 mL). The reaction mixture was stirred overnight at 80 °C and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/hexane (1 :5) to afford fe/ -butyl (4-(2-(((benzyloxy)carbonyl)amino)ethyl)-3-fluorophenyl)carbamate as a white solid (5 g, 47%). LCMS (ESI, m/zy. 411 [M+Naf.
177
144628010 vl Step 3. Benzyl (4-amino-2-fluorophenethyl)carbamate (hydrochloride salt)
[00436] Into a 100-mL round-bottom flask was added tert-butyl (4-(2-(((benzyloxy)carbonyl) amino)ethyl)-3-fluorophenyl)carbamate (5 g, 12.9 mmol) and 4 M HCl/dioxane (20 mL). The resulting solution was stirred for 2 h at RT and then concentrated in vacuo to afford benzyl (4- amino-2-fluorophenethyl)carbamate (hydrochloride salt) as a yellow solid (1.4 g, 33%). ¾- MR (300 MHz, OMSO-de) δ ppm 7.50-7.41 (m, 1H), 7.40-7.28 (m, 5H), 7.21-7.09 (m, 2H),
5.04 (s, 2H), 3.38 (t, J= 6.9 Hz, 2H) 2.90 (t, J= 6.9 Hz, 2H).
Step 4. Benzyl (2-fluoro-4-hydrazinylphenethyl)carbamate
[00437] Into a 250-mL 3 -necked round-bottom flask was added benzyl (4-amino-2- fluorophenethyl)carbamate (HC1 salt; 2.70 g, 8.31 mmol) and aqueous HC1 (12 M, 30 mL). This was followed by the addition of a solution of NaNC in H2O (0.860 g in 2 mL) at 0 °C over
1.5 h. A solution of
Figure imgf000179_0001
in H2O (7.5 g in 10 mL) was added at 0 °C over 3 h and then the resulting solution was warmed and stirred overnight at RT. The reaction was diluted by the addition of water (50 mL). The pH of the solution was adjusted to approximately 7 with ammonium hydroxide and then extracted with ethyl acetate (3 x 80 mL). The combined organic layers were washed with water (50 mL) and brine (50 raL). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford benzyl (2-fluoro-4- hydrazinylphenethyl)carba ate as a yellow oil (0,5 g, 20%). LCMS (ESI, m/z): 304 [M+H]+.
Step 5. terf-Butyl l-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-3-fluorophenyl)-4,6- dihydropyrrolo[3,4-c]pyrazole-5(li-)-carboxylate
[00438] Into a 50-mL round-bottom flask was added benzyl (2-fluoro-4- hydrazinylphenethyl)carbamate (0.30 g, 0.99 mmol), tert-butyl (3£)-3- [(dimethylamino)methylidene]-4-oxopyrrolidine-l-carboxylate (0.243 g, 1.01 mmol) and ethanoi (10 mL), The resulting solution was stirred overnight at 11.0 °C. The reaction mixture was concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 1 ) to afford tert-butyl l-(4-(2- (((benzyloxy)carbonyl) amino)ethyl)-3-fluorophenyl)-4,6-dihydropyrrolo[3,4-c]pyrazole-5(lH)- carboxylate as yellow oil (0.215 g, 45%). LCMS (ESI, m/z) 481 [M+Hf.
178
144628010 vl Example 62: Intermediate 62, fert- Butyl 4-(4-(2-(((benzyIoxy)carbonyI)amino) ethyl)phenyl)hexahydropyrrolof3,2-6]pyri le-l(2H)-carboxylate
Figure imgf000180_0001
[00439] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added benzyl N-[2-(4-bromophenyl)ethyl]carbamate (0.788 g, 2.36 mmol), toluene (50 mL), XPhos (0.225 g, 0.470 mmol), Pd(OAc): (0.053 g, 0.24 mmol), cesium carbonate (2.30 g, 7.04 mmol), and tert-butyl octahydropyrrolo[3,2-£]pyrrole-l-carboxylate (0.500 g, 2.36 mmol). The reaction mixture was stirred for 2 h at 100 °C and then cooled and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford tert-butyl 4-(4-(2-(((benzyloxy) carbonyl)amino)ethyl)phenyl)hexahydropyrrolo[3 ,2-Z>]pyrrole- 1 (2i/)~carboxylate as a yellow oil (0.700 g , 64). LCMS (ESI, mil): 466 | M l l i
Example 63: Intermediate 63. tert-butyl 3-(4-bromo-2-cyanophenyl)-3,8-diazabicyclo
[3.2.1]octane-8-carboxylate
Figure imgf000180_0002
iniemsediate S3
Into a 50-mL round-bottom flask was added 5-bromo-2~fluorobenzonitrile (1.03 g, 5.17 mmol), tert-butyl 3,8-diazabicyc!o[3.2.1]octane-8-carboxylate (1 .00 g, 4.71 mmol), potassium carbonate (1.30 g, 9.43 mmol), and DMA (10 mL). The reaction mixture was stirred for 3 h at 100 °C and then cooled and extracted with ethyl acetate (3 15 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford tert-butyl 3-(4-bromo-2-cyanophenyl)-3,8-diazabicycio[3.2.1]octane-8- carboxylate as a light yellow oil (196 nig. 11%). LCMS (ESI, m/z) 392, 394 j M I I ] .
179
144628010 vl ntermedtate 64. tert-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethy.) razolo [1 ,5-a] pyrazine-
Figure imgf000181_0001
Ste 7. Benzyl (4-(4,4,5,5~tetramethyl~l,3,2-dioxaborolan~2-yl)phenethyI)carbamate
[00441] Into a 100-mL round-bottom flask was added benzyl (4-bromophenethyl)carbamate (2.00 g, 6.00 mmol), bis(pinacolato)diboron (2.30 g, 9.10 mmol), Pd(dppf)Cl2 (0.440 g, 0.60 mraol), and KOAc ( i .50 g, 15.0 mmol). The flask was evacuated and flushed with nitrogen (3 times). Toluene (30 mL; degassed with nitrogen) was added and the resulting mixture was stirred overnight at 100 °C. The reaction was cooled then concentrated in vacuo. The resulting crude product was purified by FCC e!uting with ethyl acetate/petroleum ether (1 :5) to afford benzyl (4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenethyl)carbamate as a yellow oil (2.16 g, 95%). LCMS (ESI, m/z): 382 j \i ! ! j .
Step 2, feff-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-6,7-dihydropyrazolo [l,5-a]pyrazine-5(4fl)-carboxylate
[00442] Into a 50-mL round-bottom flask was added tert-hutyl 3-bromo~4H,5H,6H H- pyrazoio[l,5-a]pyrazine-5-carboxyiate (0.300 g, 0.99 mmol), benzyl (4-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)phenethyl)carbamate (0.380 g, 1.00 mmol), Pd (dppfJCh-C fcC (0.073 g, 0.10 mmol), CS2CO3 (0.972 g, 2.98 mmol), dioxane (5 mL), and water (0.5 mL). The reaction mixture was sparged with nitrogen and stirred overnight at 100 °C, and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford fe/ -butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)
180
144628010 vl phenyl)-6,7-dihydropyrazoio[l,5- ]pyrazine-5(4H)-carboxylate as a yellow oil (300 mg, 63%). LCMS (ESI, m/z): Ml [M+H]+.
[00443] The Intermediates in Table 8 below were synthesized according to the procedures outlined above for Example 49, Intermediate 49, using the appropriate synthetic precursors. For some examples below, both the palladium-catalyzed BFs salt coupling and hydrogenolysis were performed (Method A), and for others only hydrogenolysis (Method B).
Table 8:
Figure imgf000182_0001
181
144628010 vl
Figure imgf000183_0001
Intermediate 65-18: fert-butyl 8-(4-(2- feri-butyl 8-(4-(2- 332
182
144628010 vl
Figure imgf000184_0001
Example 66: Intermediate 66: ferf-Butyl 4-(4-(2-aminoethyl)-2,5-difluorophenyl)-3,6- dihydropyridine-l(2H)-carboxylate
Figure imgf000184_0002
intermediate 66
Step 1. ferf-Butyl 4-(4-bromo-2,5-difluorophenyl)-3,6-dihydropyridine-l(2ff)-carboxyIate
[00444] Into a 50-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 1 ,4-dibromo-2,5-difiuorobenzene (1.50 g, 5.52 mmol), tert- butyl 4-(tetramethyl-l,3,2-dioxaborolan-2-yl)-l,2,3,6-tetrahydropyridine-l-carboxylate (1.88 g, 6.06 mmol), potassium carbonate (2.28 g, 16.5 mmol), Pd(dppf)Cb'CH2Cl2 (400 mg, 0.49 mmol), DMF (20 mL) and H2O (2 mL). The reaction mixture was stirred for 2 h at 50 °C and then cooled and quenched with water (20 mL). The resulting solution was extracted with ethyl acetate (3 x 50 mL). The combined organic layers were washed with water (50 mL) and brine (50 mL). The organic layer was then concentrated in vacuo. The resulting crude product was purified by FCC eiuting with petroleum ether/ethyl acetate (5: 1) to afford fer/-butyl 4-(4-bromo- 2,5-difluorophenyl)-3,6-dihydropyridine-l(2 )-carboxylate (1.5 g, 73%). !H-NMR (300 M!¾ ( DO δ ppm 7.21-7.29 (m, 1H), 6.93-7.08 (m, 1H), 5.81-5.61 (m, 1H), 4.01-4.14 (m, 2H), 3.62 (t, J - 5,7 Hz, 2H), 2.48-2.51 (m, 2H), 1 ,50 (s, 9H).
183
144628010 vl Step 2. tert-Butyl 4-(4-(2-(((benz Ioxy)carbon I)amino)ethyI)-2,5-difluorophenyl)-3,6- dihydropyridine-l(2H)-carboxylate
[00445] Into a 100-mL round-bottom flask purged and maintained under an atmophere of nitrogen was added tert-bu yl 4-(4-bromo-2,5-difluorophenyl)-3,6-dihydropyridine-l(2H)- carboxylate (0.440 g, 1.18 rnmol), potassium (2^benzyloxycarbony!aramo)ethy i ) iriiluoroborate (0.504 g, 1.77 mmol), toluene/H20 (24/8 mL), CsiCCh (1.15 g, 3.53 rnmol), Pd^ppfjCk Cft i?. (0.193 g, 0.236 mmol), and RuPhos (0.220 g, 0.471 mmol). The resulting solution was stirred for 3 h at 95 °C and then concentrated in vacuo to afford the crude product that was purified by FCC eluting with petroleum ether/EtOAc to afford feri-butyl 4-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorop
as a yellow oil (45%). ! l-W!R (300 MHz, CDiOD) δ ppm 7.20-7.46 (m, 5H), 6.81-7.18 (m, 2H), 5.85-6.10 (m, 1H), 5.06 (s, 2H), 4.71-4.98 (m, 4H), 3.90-4.20 (m, 2H), 3 ,61 (t, J = 5.4 Hz, 2H), 3.30-3.45 (m, 2H), 2,82 (t, J = 6.9 Hz, 2H), 1.51 (s, 9H).
Step 3, tert-Butyl 4-(4-(2-aminoethyI)-2,5-difluorophenyl)-3,6-dihydropyridine-l(2H)- carboxylate
[00446] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added fert-butyl 4-(4-(2-(((benz^rloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl)-3,6-dihydropyridine- l (2H)-carboxylate (0.200 g, 0,42 mmo!), 20% Pd(OH)2/C (0.050 g), and methanol (8 mL), The resulting mixture was sparged with hydrogen gas and then stirred for 3 h at RT under hydrogen atmosphere. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford fer -butyi 4-(4-(2-aminoethyl)-2,5- difluorophenyl)-3,6-dihydropyridine-l(2i )-carboxylate as a yellow oil (200 mg). The material was used without further purification. LCMS (ESI, m/z' ): 339 [M+H]+.
184
144628010 vl Example 67: Intermediate 67. 2-(4-(l- ethylpiperidin-4-yi)phenyl)ethan-l-aniine
Figure imgf000186_0001
intermediate 67
Step 1. Benzyl (4-(I -methyl-1 ,2,3,6-tetrahydropyridin-4-yl)phenethyI)carbamate
[00447] To a one dram vial was added benzyl 4-bromophenethylcarbamate (1.50 g, 4.49 mmol), l-methyl-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-l,2,3,6-tetrahydropyridine (1.00 g, 4.49 mmol), potassium phosphate tribasic (3.8 1 g, 18.0 mmol), and XPhos 2nd generation Pd precatalyst (0.353 g, 0.449 mmol). The flask was backfilled with dry nitrogen 3 times and degassed dioxane (9.8 ml) and water (2,80 ml) were added. The reaction mixture was heated to 90 °C overnight and then cooled to RT, filtered through a bed of Celite, and concentrated m vacuo. The resulting crude product was purified by FCC eluting with 20 - 30% EtOAc in hexanes to afford benzyl (4-(l-methyl-l,2,3,6-tetrahydropyridin-4- yl)phenethyl)carbamate as a white solid (1.2 g, 77%). I CMS (ESI, m/z): 351 | \! H j
Step 2. 2~(4-(l- ei ylpiper8din-4-y!)p e!iyI)etha8i-l-aiBi8ie
[00448] To a suspension of benzyl 4-(l-methyl-l,2,3,6-tetrahydropyridin-4-yl) phenethyl carbamate (1.2 g, 3.42 mmol) in MeOH (25 ml) under an atmophere of nitrogen was added 20% palladium hydroxide/carbon (0.200 g, 1 ,42 mmol). The reaction was placed under a hydrogen atmosphere and stirred for 3 hours. The reaction was vented to nitrogen and filtered through Celite, washing the filter cake with EtOAc (~ 200 mL). The filtrate was concentrated in vacuo to afford 2-(4-(l-methylpiperidin-4-yl)phenyl)ethan-l-amine as a white solid (724 mg, 97%). LCMS (ESI, m/z) 219 [M+H]+.
185
144628010 vl Example 68-1 : Intermediate 6?
[3.1,l]heptane-3-carboxyIate
Figure imgf000187_0001
dioxane, 90
Step 1
Step J. fert-Butyl 6-(4-(2-(((benzyloxy)carbonyI)amino)ethyI)phenyl)-3,6-diazabicyclo[3.1.1] heptane-3-carboxylate
[00449] To a vial was added benzyl 4-bromophenethylcarbamate (0.337 g, 1.01 mrnol), tert- butyl 3,6-diazabicyclo[3.1.1]heptane-3-carboxylate (0.200 g, 1.01 mmoi), CS2CO3 (0.822 g, 2.52 mmoi), and XPhos Pd 2nd generation precatalyst (0.079 g. 0.10 mmoi). The vial was backfilled with dry nitrogen 3 times. Dioxane (2 ml) was added to the solid mixture and a stream of dry nitrogen was bubbled through the reaction for 2 minutes. The reaction mixture was heated to 90 °C overnight and then cooled to RT, filtered through Celite and concentrated in vacuo. The resulting crude product was purified by FCC eluting with 20 - 30% EtOAc in hexanes to afford teri-\mXy\ 6-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-3,6-diazabicyclo[3.1 .1 ]heptane~3- carboxylate as a white solid (322 mg, 71%). LCMS (ESI, m/z): 452 [M÷H]+.
Step 2, terf-Butyl 6-(4-(2-aminoethyl)phenyI)-3,6-diazabicyclo[3.1.1]heptane-3-carboxylate
[00450] To a solution of tert-buty\ 6-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-3,6- diazabicyclo[3.1.1]heptane-3-carboxylate (0.322 g, 0.714 mmoi) in methanol (20 nil) under an atmophere of nitrogen was added 20% palladium hydroxide on carbon (100 mg, 0.714 mmoi). The reaction was placed under a hydrogen atmosphere (balloon) and stirred for 3 h. The reaction was vented to nitrogen and filtered through Celite. The filter cake was washed with EtOAc (~ 80 mL) and the filtrate was concentrated in vacuo to afford tert-buty] 6-(4-(2-aminoethyl)phenyl)- 3,6-diazabicyclo[3.1.1]heptane-3-carboxylate as a light yellow oil (99%) that was carried on without further purification. LCMS (ESI, m/z): 318 [M+H]+.
186
144628010 vl [00451] The intermediates in Table 9 below were synthesized according to the procedures outlined above for Example 68-1, Intermediate 68-1, using the appropriate synthetic precursors.
Table 9:
Figure imgf000188_0002
Example 69: Intermediate 69. ieri-Butyl 3-(4-(2-aminoethyl)-2-chlorophenyl)-3,8- diazabicyclo [3.2.1] octane-8-carboxylate
Figure imgf000188_0001
Step I. ferf-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)-2-chlorophenyl)-3,8- diazabicyclo[3.2.1]octaiie-8~carboxylate
[00452] Into a 50-mL round-bottom flask was added tot-butyl 3-[4-(2- [[(benzyloxy)carbonyl]amino]ethyl)phenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0.500 g, 1.07 mmol), NCS (0.140 g, 1.05 mmol), and dichlororaethane (5 mL). The reaction mixture was stilted for 27 h at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with petroleum ether/ethyl acetate (3: 1) to afford toY-butyl 3-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)-2-chlorophenyl)-3,8-diazabicyclo[3.2.1]octane-8- carboxylate as a yellow oil (380 mg, 71%). LCMS (ESI, m/z) 500 [M+H]÷.
187
144628010 vl Step 2, tert-B tyl 3-(4-(2-aminoeth i)-2-chiorophenyI)-3,8-diazabicycIo[3.2.1]octane-8-
[00453] Into a 50-mL round-bottom flask was added tert-butyl 3-(4-(2-(((benzyloxy)carbo amino)ethyl)-2-chlorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0.38 g, 0.76 mmol), potassium hydroxide solution (40% aqueous, 5 mL), and MeOH (10 mL). The resulting solution was stirred for 4 h at 80 °C and then cooled and concentrated in vacuo to afford tert-hutyl 3-(4- (2-aminoethyl)-2-chlorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a brown oil (300 mg). The material was used without further purification, LCMS (ESI, m/z) 366 [M+H]+.
Example 70: intermediate 70, tert-Butyl 4-(4-(2-aminoethyI)-3-methylphenyl)piperazine-l-
Figure imgf000189_0001
Siep 1 Boc intermediate 70
1. terf-Butyl 4-(4-(cyanomethyl)-3-methylphenyl)piperazine-l-carboxylate
[00454] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added 2-(4-bromo-2-memylphenyl)acetonitrile (2.65 g, 12.6 mmol), tert-butyl piperazine-l-carboxylate (2.83 g, 15.2 mmol), Pd(OAc)2 (0.568 g, 2.54 mmol), XPhos (2.42 g, 5.07 mmol), CsiCCh (8.45 g, 25.9 mmol), and toluene (30 mL). The reaction mixture was stirred overnight at 105 °C in an oil bath and then cooled and concentrated in vacuo to provide a crade product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :3) to afford ten-butyl 4-(4-(cyanomethyl)-3-methylphenyl)piperazine-l-carboxylate as a yellow solid (1.3 g, 33%). LCMS (ESI, m/z): 316 j M I ! j
Step 2. tert-Butyl 4-(4-(2-aminoethyi)-3-methylphenyl)piperazine-l-carboxylate
[00455] Into a 100-mL round-bottom flask, purged and maintained with nitrogen, was added tert-butyl 4-(4-(cyanomethyl)-3-methylphenyl)piperazine-l-carboxylate (1 ,30 g, 4.12 mmol), Raney Ni (500 mg), and methanol (20 mL). The resulting mixture was sparged with hydrogen and was stirred overnight at RT under hydrogen (balloon). The reaction was vented to nitrogen and the solids were removed bv filtration. The filtrate was concentrated in vacuo to afford tert-
188
144628010 vl butyl 4-(4-(2-aminoethyl)-3-methylphenyl)piperazine-l-carboxylate as a yellow oil (400 mg, 30%). LCMS (ESI, m/z) 320 i\! - H]
[00456] Example 71-1: Intermediate 71-1. fcrt-Butyl 4-(4-(2-aminoethyI)-2-iluorophenyl)- 3,6-dihydropyridine-l(2/ )-carboxylate
Figure imgf000190_0001
Step 1
Step 1. feri-Butyl 4-(4-(cyanomethyl)-2-fluorophenyl)-3,6-dihydropyridine-l(2J
[00457] Into a 100-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added 2-(4-bromo-3-fluorophenyl)acetonitrile (0.500 g, 2.34 mmol), feri-butyl 4-(tetramethyl-l,3,2-dioxaborolan-2-yl)-l,2,3,6-tetrahydropyridine-l-carboxylate (1.09 g, 3.53 mmol), potassium carbonate (0.972 g, 7.03 mmol), Pd(dppf)Cl2 CH2CI2 (0.384 g, 0.47 mmol), DM! (20 mL) and H2O (2 mL). The reaction mixture was stirred at 80 °C for 3h and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford fcrt-butyl 4-(4-(cyanomethyl)-2-fluorophenyl)-3,6- dihydropyri dine- 1 (2H)-carboxylate as a yellow solid (0.7 g, 95%). lH-NMR (300 MHz, CDC ) δ ppm 7.33-7.23 (m, 1H), 7.18-7.00 (m, 2H), 5.97 (s, 1H), 4. 1 7-4.06 (m, 2H), 3.81-3.70 (m, 21 1), 3.64 (t, J = 5.5 Hz, 2H), 2.51-2.46 (m, 2H), 1.5 (s, 9H).
Step 2. ferf-Butyl 4-(4-(2-aminoethyl)-2-fluorophenyl)-3,6-dihydropyridine-l(2H)-
38] Into a 50-mL high pressure reactor that was purged and maintained under an inert atmosphere of nitrogen was added tert~hxA\'\ 4-(4-(cyanomethyl)-2-fiuorophenyl)-3,6- dihydropyridine-l(2H)-carboxylate (0.300 g, 0.95 mmol), Raney Ni (0.2 g), and NH3/EtOH (4 N; 25 mL). The reaction vessel was pressurized with hydrogen (10 aim) and the mixture was stirred overnight at 35 °C. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford fer/-butyl 4-(4-(2-
189
144628010 vl aminoethyl)-2-fiuorophenyl)-3,6-dihydropyridine-l(2H)-carboxylate as a light yellow oil (440 mg). The material was carried on without further purification. LCMS (ESI, m/z)\ 321 [M÷H] .
[00459] The Intermediate in Table 10 below was synthesized according to the procedures outlined above for Example 71-1, Intermediate 71-1, using the appropriate synthetic precursors.
Table 10:
Figure imgf000191_0002
Example 72: Intermediate 72. tert-B tyl 3-(4-(2-aminoethyl)-3-chlorophenyl)-3,. diazabicy do [3.2,1 ] octan e-8-carboxylate
Figure imgf000191_0001
intermediate 7:
Step 1. tert-Butyl 3-(3-chloro-4-formyIphenyl)-3,8-diazabicycIo[3.2.1]octane-8-carboxylate
[00460] Into a 100-mL round-bottom flask was added 2-chloro-4-fluorobenzaldehyde (0.743 g, 4.69 rnmol), tert-h tyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate (1 .00 g, 4.71 mmoi), potassium carbonate (1.30 g, 9.41 mmol), and DMF (15 mL). The reaction mixture was stirred for 3 h at 100 °C and then cooled to RT and quenched with water (50 mL). The resulting solution was extracted with ethyl acetate (3 x 20 mL) and the combined organic layers were dried over anhydrous sodium sulfate. The solids were removed by filtration over Celite and the filtrate was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford fe/ -butyl 3-(3-chloro-4-formylphenyl)-3,8-
190
144628010 vl diazabicyclo[3.2.1 ]octane-8-carboxyiate as a white solid (640 rag, 39%). LCMS (ESI, m/z): 351 \ \ I i f ! .
Step 2. tert-Butyl 3-(3-chloro-4-(cyanomethyl)phenyl)-3,8-diazabicycIo[3.2.1]octane-8- carboxylate
[00461] Into a 250-mL 3 -necked round-bottom flask purged and maintained with nitrogen atmosphere was added KOt-Bu (0,820 g, 7.32 ramol) and tetrahydrofuran (60 mL). The resulting solution was cooled to -78 °C and treated with a solution of p-toluenesulfonyl isocyanide (0.713 g, 3.94 rnmol) in THE (2 mL). The resulting solution was stirred for 15 min. This was followed by the addition of a solution of tert-butyl 3-(3-chloro-4-formylphenyl)-3,8- diazabicyclo[3.2.1 ]octane-8-carboxylate (640 mg, 1.82 rnmol) in THF (2 mL). The resulting mixture was stirred for 2 h at -78 °C and then methanol (5 mL) was added. The resulting solution was heated to 80 °C for 30 min and then cooled and concentrated in vacuo. The crude product was purified by FCC eiuting with ethyl acetate/petroleum ether (1 :3) to afford the title compound as a yellow solid (240 mg, 36%). LCMS (ESI, m/z): 362 | \l - H j \
Step 3. tert-Butyl 3-(4-(2-aminoethyl)-3-chlorophenyl)-3,8-diazabicyclof3.2.1]octane-8- car boxy late
[00462] Into a 50-mL round-bottom flask that was purged and maintained with nitrogen was added tert-hutyl 3-(3-chloro-4-(cyanomethyl)phenyl)-3,8-diazabicyclo[3.2. l]octane-8- carboxylate (0.22g, 0.61 rnmol) followed by BH3/THF (1.0 M, 10 mL). The resulting solution was stirred for 3 h at 70 °C and then cooled to RT and quenched with methanol (I mL). The resulting mixture was concentrated in vacuo to afford tert-butyl 3-(4-(2-aminoethyl)-3- chlorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a white solid (223 mg) that was used without further purification. LCMS (ESI, m/z): 366 | M H i ' .
191
144628010 vl Example 73: 1 si ter mediate 73. ieri- Butyl 4-(4-(2-aminoethyi)-2,3-difluorophenyl) piperazine-l-carboxylate
Figure imgf000193_0001
intermediate 73
Step 1. 4-(2,3-difluoro-4-formylphenyl)piperazine-l-carboxylate
[00463] The title compound was prepared according to the procedure used for Step 1 of the preparation of Example 72: Inter mediate 72 at 90 °C using 2,3,4-trifiuorobenzaldehyde. This afforded the title compound as a white solid (46%), LCMS (ESI, m/z): 327 [M÷H]÷.
Step 2. ieri-Butyl 4-(4-(cyanomethyI)-2,3-difluorophenyl)piperazine-l-carboxylate
[00464] Into a 100-mL round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added a solution of i-BuOK (1 .03 g, 9, 18 mmol) in tetrahydrofuran (10 mL). The solution was cooled to -78 °C and 4-methylbenzene-l-sulfonyl isocyanide (1.00 g, 5,46 mmol) in ethylene glycol dimethyl ether (10 mL) and a solution of ieri-butyl 4-(2,3- difluoro-4-formylphenyl)piperazine-l-carboxylate (1.50 g, 4.60 mmol) in ethylene glycol dimethyl ether (15 mL) was added at -78 °C, The reaction mixture was stirred for 4 h at -78 °C and then methanol ( 15 mL) was added. The resulting solution was heated and stirred overnight at 80 °C and then cooled and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford tert-butyl 4-(4-(cyanomethyl)-2,3- difluorophenyI)piperazine-l-carboxylate as a white solid (900 mg, 58%). LCMS (ESI, m/z): 338 \ \ I i f ! .
Step 3. ieri-Butyl 4-(4-(2-aminoethyl)-2,3-difluorophenyI)piperaziiie-l-carboxylate
[00465] Into a 50-mL round-bottom flask, that was purged and maintained with nitrogen was added ieri-butyl 4-(4-(cyanomethyl)-2,3-difluorophenyl)piperazine-l-carboxylate (0.200 g, 0,590 mmol) and tetrahydrofuran (5 mL). To the solution was added BH3 (1 M in THF; 2 mL, 2 mmol). The resulting solution was stirred overnight at 70 °C and then cooled to RT and
192
144628010 vl quenched with methanol (10 mL). The resulting mixture was concentrated in vacuo to afford terl-hutyl 4-(4-(2-aminoethyl)-2,3-difluorophenyl) piperazine-l-carboxyiate as a colorless oil (120 mg). The material was used without further purification, LCMS (ESI, m/'z): 342[M+Fi]~,
Example 74: Intermediate 74. fe/tf-Butyl 4-(5-(2-aminoethyl)-[l,l'-biphenyl]-2- yI)piperazine-l-carboxylate
Figure imgf000194_0001
intermediate 74
Step 1, krt-Uulyl 4-(2-bromo-4-formylphenyl)piperazine-l-carboxyIate
[00466] The title compound was prepared according to the procedure used for Step 1 of the preparation of Example 72: Intermediate 72, using 3-bromo-4-fluorobenzaldehyde as the starting material. This afforded the title compound as a light yellow solid (52%). LCMS (ESI, m/z . 369, 371 \ \ \ \ \~
Step 2. 4-(5-formyl-[l,l'-biphenyl]-2-yl)piperazine-l-carboxylate
[00467] Into a 50-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-butyl 4-(2-bromo-4-formylphenyI)piperazine-l-carboxylate (2, 14 g, 5,80 mmol), phenylboronic acid (0.848 g, 6.95 mmol), 3PO4 (4.15 g, 15.8 mmol), Pd(dppf)Ch (0.42 g, 0.57 mmol), and DMF (25 mL). The reaction mixture was stirred for 12 h at 100 °C and then cooled to RT. The resulting mixture was quenched with H2O (50-mL) and extracted with dichloromethane (2 x 25 mL). The combined organic layers were washed with brine (10 mL), concentrated in vacuo, and the crude product was purified by FCC eiuting with ethyl acetate/petroleum ether (1 :6) to afford fert-butyl 4-(5-formyl-[l, l'-biphenyl]-2-yl)piperazine-l- carboxylate as a brown solid (1.8 g, 85%). LCMS (ESI, m/z): 367 [M+H]+.
193
144628010 vl Step 3. tert-But l 4-(5-(cyanomethyI)-[l,l'-biphenyl]-2-yl)piperazine-l-carboxyIate
[00468] Into a 50-mL 3 -necked round-bottom flask was added a solution of KOt-Bu (0.610 g, 5.44 mmol) in tetrahydrofuran (3 mL). This was followed by the addition of a solution of TosMIC (0.590 g, 2.99 mmol) in ethylene glycol dimethyl ether (4 mL) at -50 °C. To this was added a solution of fer/-butyl 4-(5-formyl-[l, r-biphenyl]-2-yl)piperazine-l -carboxylate (1.00 g, 2.73 mmol) in ethylene glycol dimethyl ether (4 mL) dropwise with stirring at -50 °C over 15 min. The resulting solution was stirred for 2 h at 0 °C and then heated to 80 °C and stirred for 30 minutes, The reaction mixture was cooled and concentrated in vacuo to provide a crude product that was dissolved in DCM (30 mL) and washed with brine (3 x 10 mL). The organic layer was then concentrated in vacuo and the crude product was purified by FCC eluting with ethyl acetate/petroleum ether (2:3) to afford fert-butyl 4-(5-(cyanomethyl)-[l, l'-biphenyl]-2- yi)piperazine-l -carboxylate as a yellow solid (220 mg, 21%). LCMS (ESI, m/z): 378 [M+H]÷.
Step 4. ieri-Butyl 4-(5-(2-aminoethyl)-[I ,1 '-biphenylj-2-yl)piperazine-l-carboxylate
[00469] Into a 50-mL high pressure tank reactor that was purged and maintained with nitrogen was added /erf-butyl 4-(5-(cyanomethyl)-[l,'l'-biphenyl]-2-yl)piperazine-l-carboxylate (0.230 g, 0,61 mmol), Raney Ni (3.5 nig), and 4 M NL /metha ol (20 mL). The reaction vessel was pressurized with hydrogen (10 atm), stirred for 12 h at RT, and then vented with nitrogen. The solids were removed by filtration over Celite and the filtrate was concentrated in vacuo to afford tert-butyl 4-(5-(2-aminoethyl)-[l, l'-biphenyl]-2-yl)piperazine-l-carboxylate as a white solid (180 mg, 77%). LCMS (ESI, m/z): 382 [M+H]+.
Example 75: intermediate 75. teri- Butyl 3-(4-(2-aminoethyI)phenyI)-3-hydroxypyrrolidine- 1-carboxylate
Figure imgf000195_0001
intermediate 75
Step 2
Step 1. l-(4-Bromophenethyl)-2,2,5,5-tetramethyl-l,2,5-azadisiloIidine
[00470] Into a 50-mL round-bottom flask was added 2-(4-bromophenyl)ethan-l-amine (0.500 g, 2.50 mmol) and dichloromethane (15 mL). This was followed by the addition of tri ethyl amine
194
144628010 vl (0.505 g, 0.696 mL, 5.00 mmol) dropwise with stirring. Chloro [2-(chlorodimethylsiiyl)ethyl] dimethylsilane (0.538 g, 2.50 mmol) was then added portion-wise. The resulting solution was stirred for 2 h at RT and then extracted with ethyl acetate (3 x 20 mL). The combined organic layers were washed with brine (20 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford l-(4-bromophenethyl)-2,2,5,5-tetratneihyl-l ,2,5-azadisijoiidine as a white solid (600 mg). The material was used without further purification.
Step 2. ferf-Butyl 3-(4-(2-aminoethyl)phenyI)-3-hydroxypyrrolidine-l-carboxylate
[00471] Into a 250-mL 3-necked round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added l-(4-bromophenethyl)-2,2,5,5-tetramethyl-l ,2,5- azadisilolidine (2,06 g, 6.02 mmol) and tetrahydrofuran (30 mL). The resulting solution was cooled -70 °C and then w-BuLi (2.5 M, 2,6 ml.) was added dropwise with stirring. The resulting solution was stirred for 0.5 h and then fert-butyl 3-oxopyrrolidine-l-carboxylate (1.10 g, 5.94 mmol), was added portion-wise. The resulting solution was stirred for 2 h at -70 °C and then quenched with saturated aqueous H4CI solution (50-niL). The mixture was extracted with dichloromethane (3 x 50-mL) and the combined organic layers were washed with brine (1 x 50- mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford tert-buXyl 3-(4-(2-aminoethyl)phenyl)-3-hydroxypyrrolidine-l -carboxylate as a brown solid (1 ,0 g, 55%). The material was used without further purification. LCMS (ESI, m/z): 307 [M+H]+. le 76-1: Intermediate 76-1. ferf-Butyl 3-[4-(l-aminopropan-2-yl)-2,5- difluorophenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate
Figure imgf000196_0001
Step 4
intermediate 76-1
195
144628010 vl Step 1. teii-Eutjl 3-(4-bromo-2,5-difluorophenyI)-3,8-diazabicycIo[3.2.1]octane-8- carboxylate
[00472] Into a 250-mL round-bottom flask that was purged and maintained under an inert, atmosphere of nitrogen was added l,4-dibromo-2,5-difluorobenzene (2.97 g, 10.9 mmol), tert- butyl 3,8-diazabicyclo[3.2.1]oetane-8-carboxylate (2.12 g, 9.99 mmol), Pd2(dba)3-CHCb (0.52 g, 0.50 mmol), XantPhos (0.578 g, 1.00 mmol), sodium i-butoxide (1.92 g, 20.0 mmol), and toluene (100 mL). The resulting mixture was stirred for 45 min at 70 °C and then cooled to RT and quenched with water (20 mL). The resulting solution was extracted with ethyl acetate (3 x 20 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether EA/PE = 1 : 100 to 1 :5) to afford ferf-butyl 3 -(4- bromo-2,5-difluorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a light yellow solid (2.6 g, 58%). LCMS (ESI, m/z): 403, 405 [M+H]+.
Step 2, ferf- uiyl 3-(4-acetyl-2,5-difluorophenyI)-3,8-diazabicyclo [3.2.1 ]ociane-8- carboxylate
[00473] Into a 250-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added feri-butyl 3-(4-bromo-2,5-difluorophenyl)-3,8-diazabicyclo[3.2.1]octane- 8-carboxylate (1 .30 g, 3.22 mmol), tributyl(l-ethoxyethenyl)stannane (1.75 g, 1.64 mL, 4.85 mmol), Pd(dppf)Cl2 CH2Cl2 (0.53 g, 0.64 mmol), DIEA (1.25 g, 1.68 mL, 9.67 mmol), and toluene (100 mL). The resulting solution was stirred for 18 h at 100 CC and then cooled to RT and quenched with saturated aqueous ammonium chloride solution (30 mL). The resulting mixture was extracted with ethyl acetate (3 x 100 ml,) and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to provide a caide product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (EA/PE = 1 : 100 to 1 : 10) to afford iert-butyl 3-(4-aGetyl-2,5-difluorophenyl)-3,8-diazabicyclo[3.2.1]octane- 8-carboxylate as light yellow oil (460 mg, 35%). LCMS (ESI, m/z): 367 [M+H]+.
Step 3, ferf-Butyl 3-[4-(l-cyanoethyl)-2,5-difluorophenyl]-3,8-diazabicycIo[3.2.1]octane-8- carboxylate
[00474] Into a 100-mL round-bottom flask was added tert-butyl 3-(4-acetyl-2,5- difluorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0.420 g, 1.15 mmol), TosMIC
196
144628010 vl (0.336 g, 1.71 mmol), potassium t-butoxide (0.321 g, 2.86 mmol), t-butanol (10 mL), and ethylene glycol dimethyl ether (10 mL). The resulting solution was stirred for 18 h at 90 °C and then cooled to RT and quenched with water (50 mL). The resulting mixture was extracted with ethyl acetate (3 x 20 mL). The combined organic layers were then dried over sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (EA/PE = 1 : 100 to 1 : 10) to afford tert-butyl 3~[4-(i-cyanoethyl)-2,5-difluorophenyl]-3,8-diazabicyclo[3,2. i]octane-8-carboxylate as a light yellow solid (350 mg, 73%). LCMS (ESI, m/z): 378 [M+Hf.
Step 4. ferf-Butyl 3-[4-(l-aminopropan-2-yI)-2,5-difluorophenyl]-3,8-diazabicyclo[3.2.1] octane-8-carboxyIate
[00475] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added tenf-butyl 3-[4-(l-cyanoethyl)-2,5-difluorophenyl]-3,8-diazabicyclo[3.2.1]octane-8-caAoxylate (0.35 g, 0,93 mmol), Raney Ni (0.35 g), and a solution of ammonia in methanol (7 M, 20 mL). The resulting mixture was sparged with hydrogen and then stirred for 2 h at 20 °C under a hydrogen atmosphere. The solids were removed by filtration through Celite and the filtrate was concentrated in vacuo to afford tert-butyl 3-[4-(l-aminopropan-2-yl)-2,5-difluorophenyl]-3,8- diazabicyclo[3 ,2.1] octane-8-carboxylate as a light yellow solid (200 mg, 51%). LCMS (ESI, m/z): 382 [M+H .
[00476] The Intermediate in Table 11 below was synthesized according to the procedures outlined above for Example 76-1, Intermediate 76-1, using the appropriate synthetic precursors.
Table 11:
Figure imgf000198_0001
197
144628010 vl Example 77-1: Intermediate 77-1. teri-Butyl 3 4-(l-aminopropan-2-yI)-3 1uoropheny]
Figure imgf000199_0001
intermediate 77-1
Step 1. ierf- Butyl 3-(4-acetyl-3-fluorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxyIate
[00477] Into a 100-mL round-bottom flask was added l-(2,4-difluorophenyl)ethan-l-one (2.65 g, 17.0 mmol), tert-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate (3.00 g, 14.1 mmol), potassium carbonate (5.86 g, 42,4 mmol), and HMPA (30 mL). The resulting solution was stirred overnight at 70 °C in an oil bath and then cooled to RT and quenched with water (30 mL). The resulting solution was extracted with ethyl acetate (3 x 30 mL). The combined organic layers were concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eiuted with ethyl acetate/petroleum ether (1 :5) to afford fer/-butyl 3-(4- acetyl-3-fluorophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as brown oil (1.8 g, 30%). LCMS (ESI, m/z): 349
2. tert-Butyl 3-[4-(l-cyanoethyl)-3-fluorophenyI]-3,8-diazabicyclo[3.2.1]octane-8-
[00478] Into a 100-mL round-bottom flask was added fe/ -butyl 3-(4-acetyl-3-fluorophenyl)-
3,8-diazabicydo[3,2J]octane~8-earboxylate (1.80 g, 5.17 mmol), K()r-Bu (1 ,45 g, 13 ,0 mmol),?-toluenesulfonyl isocyanide (1.51 g, 7.74 mmol), te/ -butanol (20 mL), and ethylene glycol dimethyl ether (20 mL). The resulting solution was stirred overnight at 90 °C in an oil bath and then cooled and quenched by the addition water (20 mL). The resulting solution was extracted with ethyl acetate (3 x 20 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eiuted with ethyl acetate/petroleum ether (1 :5) to afford tert-
198
144628010 vl butyl 3-[4-(l-cyanoethyl)-3-fluorophenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a brown oil (1.2 g, 65%). LCMS (ESI, m/z): 360 ί\Ι · Η]
Step 3, tarf-Butyl 3-[4-(l-aminopropan-2-yl)-3-fluorophenyl]-3,8-diazabicyclo[3.2.1]octane-
8-carboxyIate
[00479] Into a 100-mL round-bottom flask, purged and maintained with nitrogen, was added fe/Y-hutyl 3 -[4-(l. -cyanoethy 1 )-3 -fluoropheny I ]-3 , 8-diazabi cyclo[3.2.1 ]octane-8-carboxylate (1.00 g, 2.78 mmol), tb/MeOH (7 M, 20 mL), and Raney Ni (500 mg). The reaction mixture was sparged with hydrogen and was stirred for 2 h at RT under an atmosphere of hydrogen . The solids were removed by filtration over Celite and the filtrate was concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with dichloromethane/methanol (10: 1 ) to afford fert-butyl 3-[4-(l-aminopropan-2-yl)-3- fluorophenyl]-3,8-diazabieyclo[3.2.1 ]octane-8-carboxylaie as yellow oil (800 mg, 79%). LCMS (ESI, m/z): 364 j 1 1 11
100480] The Intermediate in Table 12 below was synthesized according to the procedures outlined above for Example 77-1, Intermediate 77-1, using the appropriate synthetic precursors.
Table 12:
Figure imgf000200_0001
199
144628010 vl Example 78: Intermediate 78. tert-Butyl 3-(4-(l-aminopropan-2-yl)-2-cyanophenyl)-3, diazabicyclo [3.2,1] octane-^
Figure imgf000201_0001
Step 1. feri-Butyl 3-(4-acetyl-2-bromophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate
[00481] Into a 20-mL microwave tube was added l-(3-bromo-4-fluorophenyl)ethan-l-one (3.00 g, 13.8 mmol), fert-butyl 3,8-diazabicyclo[3.2. I ]octane-8-carboxyiate (3.67 g, 17.3 mmol), DIEA (5.48 g, 7.39 mL, 42.4 mmol), and DMSO (8 mL). The resulting solution was heated to 120 °C for 4 h under microwave irradiation. The reaction mixture was cooled to RT and then quenched with water (30 mL). The resulting mixture was extracted with ethyl acetate (3 x 30 rnL). and the combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford fer -butyl 3-(4- acetyl-2-bromophenyl)-3,8-diazabicyclo[3.2.1 ]octane-8-carboxylate as a yello oil (1 .5 g, 27%). LCMS (ESI, m/z) 409, 411 [ \ I— H I .
Step 2. tert- utyl 3-[2-bromo-4-(l-cyanoethyl)phenyI]-3,8-diazabicyclo[3.2.1]octane-8- carboxvlate
182] into a 100-mL 3 -necked round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added potassium /-butoxide (0.480 g, 4.28 mmol) and tetrahydrofuran ( 15 mL) followed by the dropwise addition of a solution of / toluenesulfonyi isocyanide (0.500 g, 2.56 mmol) in tetrahydrofuran (3 mL) with stirring at -78 °C. The resulting solution was stirred for 15 minutes at -78 °C and then a solution of tert- vXyl 3-(4-acetyl-2- bromophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0.700 g. 1.71 mmol) in
200
144628010 vl tetrahydrofuran (5 mL) was added dropwise with stirring at -78 °C. The resulting mixture was stirred for an additional 1.5 h at this temperature. Methanol (10 mL) was added, and the resulting solution was heated to 80 °C and stirred for an additional 30 minutes. The reaction was cooled to RT and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford ferf-butyl 3-[2- bromo-4-(l-cyanoethyl)phenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a yellow oil (500 mg, 70%), LCMS (ESI, m/z) 420, 422 I I 1 11 .
Step 3. tert-Bu yl 3- 4-(l-a5Bi!iopropaii-2-yl)-2-bromophesiyl]-3,8-diazabicyclo 3,2.i]octa5ie- 8-carboxyIate
[00483] Into a 250-mL round-bottom flask that was purged and maintained with nitrogen was added tert-butyl 3-[2-bromo-4-(l-cyanoelhyl)phenyl]-3,8-diazabicyclo[3.2.1]octane-8- carboxylate (1.50 g, 3.57 mmol) and borane tetrahydrofuran complex (1 M, 20 mL, 20 mmol). The resulting solution was stirred for 2 h at 25 °C and then quenched with methanol (30 mL). The resulting mixture was concentrated in vacuo to provide a cmde product that was purified via silica gel chromatography and eluted with dichloromethane/methanol (10: 1) to afford tert-butyl 3-[4-(l-aminopropan-2-yl)-2-bromophenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a white solid (1.3 u. 86%). LCMS (ESI, m/z) 424, 426[M+H]+
Step 4, ferf-Butyl 3-[4-(l-[[(benzyloxy)carbonyl]amino]propan-2-yl)-2-bromophenyl]-3,8- diazabicycIo[3.2.1]octane-8-carboxylate
[00484] Into a 100-mL round-bottom flask was added fert-butyl 3-[4-(l-aminopropan-2-yl)-2- bromophenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (1.40 g, 3,30 mmol), potassium carbonate (1.37 g, 9.91 mmol), ethyl acetate (20 mL), water (20 mL) and CbzCl (0.619 g, 0.516 mL, 3 ,63 mmol ), The resulting mixture was stirred for 3 h at 60 °C in an oil bath and the cooled to RT. The resulting solution was diluted with water (100 mL) and extracted with ethyl acetate (3 x 20 mL). The combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 1) to afford fert-butyl 3-[4- (l -[[(benzyloxy)carbonyl]amino]propan-2-yl)-2-bromophenyl]-3,8-diazabicyclo[3.2.1]octane-8- carboxylate as a light yellow oil (1.0 g, 54%). LCMS (ESI, m/z) 558, 560[M+H]r.
201
144628010 vl Step 5. fert-Bnty! 3-[4-(l-[[(benzyloxy)carbonyllamino|propan-2-yl)-2-cyanophenyl]-3,8- diazabicyclo [3.2,1] octane-8-carboxylate
[00485] Into a 10-mL microwave tube purged and maintained under an inert atmosphere of nitrogen was placed fert-butyl 3-[4-(l-[[(benzyloxy)carbonyl]amino]propan-2-yl)-2- bromophenyl]-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0,800 mg, 1 ,43 mmol), Zn(CN)2 (0.167 g, 1.42 mmol), Pd(PPh3)4 (0.166 g, 0.14 mmol), and DMF (3 mL). The resulting mixture heated at 120 °C for 1 h under microwave irradiation. The reaction mixture was cooled to RT, quenched with water (50-mL), and extracted with ethyl acetate (3 x 10 mL). The combined organic layers were concentrated in vacuo to provide a crude product that was purified via Prep- TLC and eluted with ethyl acetate/petroleum ether (1 :3) to afford tert-butyl 3-[4-(l - [[(benzyloxy)carbonyl]amino]propan-2-yl)-2-cyanophenyl]-3,8-diazabicyclo[3.2.1]octane-8- carboxylate as colorless oil (400 mg, 55%). LCMS (ESI, m/z) 505[M+H]~
Step 6. !erS-lhityl 3-(4-(l-aminopropan-2-yI)-2-cyanophenyl)-3,8-diazabicycIo[3.2.1]octane-
8-carboxyIate
[00486] Into a 100-mL round-bottom flask purged and maintained with nitrogen was added fe -buty 1 3 - [4-( 1 -[[(benzyl oxy)carbonyl ]amino]propan-2-yl)-2-cyanophenyl]-3 , 8-diazabi cyclo [3.2. 'l]octane-8-carboxylate (0.35 g, 0.69 mmol), tetrahydrofuran (15 mL), and 10% palladium on carbon (350 mg). The reaction mixture was sparged with hydrogen and then stirred for 1 h at RT under an atmosphere of hydrogen. The solids were removed by filtration over Celite, and the filtrate was concentrated in vacuo to provide a crude product that was purified via prep-TLC and eluted with dichloromethane/methanol ( 10: 1) to afford fert-Butyf 3-(4-(l-aminopropan-2-yl)-2- cyanophenyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate as a brow oil (180 mg, 70%). LCMS (ESI, m/z) 371 [M+H]+
202
144628010 vl Example 109: Intermediate 79: terf- But l 4-(4-(l-ammopropan-2-yi)phenyl)piperazine-l-
Figure imgf000204_0001
NaOf-Bu
i
Figure imgf000204_0002
intermediate 79
Step 1. 2-(4-Bromophenyl)propanenitriIe
[00487] into a 100-mL round-bottom flask was added 2-(4-bromophenyl)acetonitrile (3.00 g, 15.3 mmol), tetrahydrofuran (30 mL), and sodium hydride (60% by weight; 2.60 g, 65.0 mmol). The reaction mixture was stirred for I h at RT. The reaction mixture was cooled to 0 °C then iodomethane (0.551 g, 0.242 mL, 3.88 mmol) was added and the reaction mixture was stirred at RT for an additional 2 h. The reaction was then quenched by the addition of H2O (10 mL), and then was extracted with dichloromethane (3 30 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 2-(4- bromophenyi)propanemtrile (2 g, 62%) as a yellow solid. LCMS (ESI, m/z) 210 [M+H]+.
Step 2, ieri- utyl 4-(4-(l-cyanoethyl)phenyl)piperazine-l-carboxylate
[00488] Into a 100-mL round-bottom flask that was purged and maintained with an inert atmosphere of nitrogen was added 2-(4-bromophenyl)propanenitrile (1.05 g, 5.00 mmol), tert- butyl piperazine-l-carboxyiate (1.02 g, 5.49 mmol), Pd(OAc)2 (0.056 g, 0.25 mmol), XPhos (0.239 g, 0.501 mmol), Na()r-Bu (0.096 g, 1.00 mmol), and toluene (20 mL). The resulting solution was stirred for 2 h at 100 °C then was cooled and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford tert-hutyl 4-(4-(l -cyanoethyl)phenyl)piperazine-l - carboxylate (600 mg, 38%) as brown oil. LCMS (ESI, m/z) 316 [M+H]".
203
144628010 vl Step 3. tert-Butyl 4-(4-(l-aminopropan-2-yl)phenyl)piperazine-l-carboxyIate
[00489] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added tert-hutyl 4-(4-(l-cyanoethyl)phenyl)piperazine-l-carboxylate (0,630 g, 2.00 mmol), Raney i (20 mg), and NTb/MeOH (7 M; 20 niL). The reaction mixture was sparged with hydrogen, and then was stirred overnight at RT under hydrogen (balloon pressure). The solids were removed by filtration over Ceiite, and the filtrate was concentrated in vacuo to afford fcri-butyl 4-(4-(l- aminopropan-2-yl)phenyl)piperazine-l-carboxylate (450 mg, 71%) as light yellow oil. LCMS (ESI, /¾/z) 320[M+H]+.
Example 110: Intermediate 80: tert-Butyl 4-(4-(2-aminoethyl)-2-chlorophenyl)piperidine-l- carboxylate
Figure imgf000205_0001
intermediate 80
Step 1. tert-Butyl 4-(2-chioro-4-formylphenyl)-3,6-dihydropyridine-l(2iT)-carboxylate
[00490] Into a 50-mL 3-necked round-bottom flask purged and maintained with nitrogen was added 4-bromo-3-chlorobenzaldehyde (1.10 g, 5.01 mmol), tert-hutyl 4-(tetramethyl-l,3,2- dioxaborolan-2-yl)-l ,2,3,6-tetrahydropyridine-l -carboxylate (2,00 g, 6.47 mmol), Pd(dppf)Cl2 (0.47 g, 0.64 mmol), K PO4 H2G (0.600 g, 2.83 mmol), tetrahydrofuran (20 mL), and water (2 mL). The reaction mixture was stirred for 1 h at 50 °C, and then was cooled to RT and concentrated in vacuo. The crude product was purified via silica gel column chromatography and
204
144628010 vl eluied with ethyl acetate/petroleum ether (1 :3) to afford tert-butyl 4-(2-chloro-4-formylphenyl)- 3,6-dihydropyridine-l(2H)-carboxylate (1.5 g, 90%) as a yellow solid. LCMS (ESI, m/z) 322
\ \\ n r .
Step 2. ferf-Butyl 4-(2-chloro-4-(hydroxymethyl)phenyl)-3,6-dihydropyridine-l(2H)- carboxylate
[0049 J] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added tert-bu l 4-(2-chloro-4-formylphenyl)-3,6-dihydropyridine-l (2H)-carboxylate (1.50 g, 4.66 mmol), Raney i (0.8 g), and tetrahydrofuran (15 ml,). The reaction mixture was sparged with hydrogen then was stirred overnight at RT under hydrogen (balloon). The solids were removed by filtration over Ceiite and the filtrate was concentrated in vacuo to afford tert-butyl 4-(2- chloro-4-(hydroxyrnethyl)phenyl)-3,6-dihydropyridine-l(2/ )-carboxylate (1.4 g, 93%) as a colorless oil that was carried on without further purification. LCMS (ESI, m/z) 324 [M i l ]
Step 3. iert-B tyl 4-(2-chloro-4-(hydroxymethy!)phenyl)piperidine-l-carboxylate
[00492] Into a 100-mL round-bottom flask purged and maintained with nitrogen was added tert-butyl 4-(2-chl oro-4-(hydroxymethyl)phenyl)-3 ,6-dihydropyridine- 1 (2H)-carboxylate (0.800 g, 2.47 mmol), methanol (10 mL), and Pt02 (0, 100 g). The reaction mixture was sparged with hydrogen then was stirred overnight at RT. The solids were removed by filtration and the filtrate was concentrated in vacuo. The cmde product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :3) to afford tert-butyl 4-(2-chloro-4- (hydroxymethyl)phenyl)piperidine-l-carboxylate (470 rng , 58%) as a yellow oil. LCMS (ESI, m/z): 326 i \H i |
Step 4. tert-Butyl 4-(2-chloro-4-(((methylsulfonyI)oxy)methyI)phenyl)piperidine-l- carboxylate
[00493] Into a 50-mL round-bottom flask was added tert-butyl 4-(2-chloro-4- (hydroxymethyi)phenyl)piperidine-l-carboxylate (0.460 g, 1.41 mmol), dichloromethane (5 mL), and triethylamine (0.429 g, 0.591 mL, 4.24 mmol). The resulting mixture was cooled to -78 °C then MsCl (0.37 g, 0.25 mL 3.22 mmol) was added dropwise over 5 min. The resulting solution was allowed to warm to RT gradually and stirred for 3 h at RT. The reaction was quenched by the addition of water (30 mL) then was extracted with dichloromethane (3 x 30 mL). The
205
144628010 vl organic layers were combined, washed with water (30 niL) and brine (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford tert-hmyl 4-(2-chloro~4- (((raethylsulfonyl)oxy)methyl)phenyl)piperidine-l-carboxylate (800 mg) as a light yellow oil that was carried on without further purification. LCMS (ESI, m/z): 404 [M+H]".
Step 5, iert-Butyl 4-(2~clsloro~4-(cyanomet yI)p ei8yI)piperidi!¾e~l~carboxylate
[00494] Into a 50-mL round-bottom flask was added teri-hutyl 4-(2-chloro-4- (((methylsulfonyl)oxy)methyl)phenyl)piperidine-l-carboxylate (0.800 g, 1.98 mmol), DMSO (4 mL), and NaCN (0.500 g, 10.2 mmol). The resulting mixture was stirred for 2 h at 60 °C then was quenched by the addition of water (30 mL). The resulting solution was extracted with ethyl acetate (3 x 30 mL). The organic layers were combined, washed with water (30 mL) and brine (30 ml,), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford tert- butyl 4-(2-chloro-4-(cyanomethyl)phenyi)piperidine-l-carboxylate (290 mg, 44%) as a yellow oil that was carried on without further purification, LCMS (ESI, m/z): 335 [ +H]+. Caution: The aqueous layers containing NaCN were carefully treaied with excess aqueous FeSCn solution prior to disposal.
Step 6. ferf-Butyl 4-(4-(2-aminoethyl)-2-chlorophenyl)piperidine-l-carboxylate
[00495] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added tert-hutyl 4-(2-chloro-4~(cyanomethyl)phenyl)piperidine~l-carboxy3ate (0.30 g, 0.90 mmol) and a solution of borane in THF (1 M, 5 mL). The resulting solution was stirred for 2 h at 70 °C then was cooled to RT and quenched by the addition of methanol (10 mL). After stirring for 30 min at RT, the resulting mixture was concentrated in vacuo to afford tert-butyl 4-(4-(2-aminoethyl)- 2-chlorophenyl)piperidine-l-carboxylate (0.4 g) as a yellow oil that was carried on without further purification, LCMS (ESI, m/z): 339 | M I ! | .
206
144628010 vl Methods for the Synthesis of Compounds of Formula (I):
Example 79-1: 3-Amino-6-methyl-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3-6]pyridine-2- carboxamide (1-1)
Figure imgf000208_0001
Step 1. Benzyl 4-(4-(2-(3-amino-6-methylthieno[2,3-6Jpyridine-2-carboxamido)ethyl) phenyl)piperazine-l-carboxylate
[00496] Into a 40 mL vial that was purged and maintained under an inert atmosphere of nitrogen was added benzyl 4-(4-(2-aminoethyl)phenyl)piperazine-l-carboxylate (hydrochloride salt, Intermediate 25; 0.570 g, 1.68 mmol) followed by anhydrous dichlorom ethane (10 mL). 3- Amino-6-raethylthieno[2,3-5]pyridine-2-carboxylic acid (Example 1: Intermediate 1, 0.350 g, 1.68 mmol) was then added, followed by EDCI (0.646 g, 3.37 mmol), HOBt (0.448 g, 3.32 mmol), and 4-dimethylaminopyridine (0,308 g, 2.52 mmol). The resulting reaction mixture was stirred overnight at reflux and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with dichloromethane/methanol (10:1) to afford benzyl 4-(4-(2-(3- amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate as a white solid (200 nig, 22%). LCMS (ESI, rn/z): 530 fM+H]+.
Step 2. 3-Amino-6-methyi-N-(4-(piperazin-l-yl)phenethyi)thieno[2,3-6]pyridine-2~ carboxamide
[00497] Into a 50 mL 3 -necked round-bottom flask that was purged and maintained under an inert atmosphere of nitrogen was added benzyl 4-(4-(2-(3-amino-6-raethylthieno[2,3-*]pyridine- 2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.100 g, 0.19 mmol). This was followed by the addition of BBn/DCM (1.0 M in DCM, 3 mL) dropwise with stirring at -20 °C. The resulting solution was warmed, stirred for 1 h at 25 °C, and then quenched with 5 mL of
207
144628010 vl methanol. The resulting mixture was concentrated in vacuo to afford a crude product thai was purified by Prep-HPLC using the following conditions (Water-2767): Column, XBridge RP18 19* 150 mm, 5 μιη; mobile phase, A: water (contains lOmM NH4HCO3 + 5% NH4OH) B: CH3CN (25% to 65% over 6 min); Flow rate: 20 mL/min; UV Detector 220nm. This afforded 3- amino-6-methyl-A7-(4-(piperazin- 1 -yl)phenethyl)thieno[2,3 -Z>]pyridine-2-carboxamide as an off- white solid (13 ,9 rag, 19%). LCMS (ESI, /z): 396 [M+H] +; lE NMR (300 MHz, DMSO-ifc) δ ppm 8.29 (d, J = 8.2 Hz, 1H), 7.68 (t, J = 5.5 Hz, 1H), 7.30 (d, J = 8.4 Hz, IH), 7.14-7.02 (m, 4H), 6.85 (d, J = 8.6 Hz, 21 1), 3.39-3.34 (m, 2H), 2.99 (dd, J = 6, 1 , 3.7 Hz, 4H), 2.82 (dd, / 6.2, 3.7 Hz, 4H), 2.78-2.69 (m, 2H), 2.58 (s, 3H).
[00498] The Examples in Table 13 below were synthesized according to the procedures outlined above for Example 79-1 (H), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appear below the examples.
Table 13:
Figure imgf000209_0001
208
144628010 vl
Figure imgf000210_0001
209
144628010 vl Example
Figure imgf000211_0001
water (containing lOum NH4HCO3 + 0,05% ammonium hydroxide) and B: C¾CN (15 % to 45% over 5 min, and then 45% to 75% over 5 mm); UV Detector: 254 nm. Notes: Step 1 was conducted with HATU and DIEA in DMF solvent. Step 2 was conducted at 0 °C. HPLC Purification Method: (Waters-Ϊ): Column: X-Bridge CI 8, 19*150nm, Sum; mobile phase, water (Xi l ;l l(O : - 0.05%Nl kf !;■(); and ACN(35% CH3CN up to 42% in 9 mm); Detector,254nm.
Example 80: 3~Amiiio-AL(3-flMoro~4~(piperaziii-l~y!)pheBet yl)-6--methyIthie!io 2,3-fe] pyridine-2-carboxamide (HQ salt) (1-10)
Figure imgf000211_0002
Example 80 (1-10)
Step 1. to"/- But l 4-(4-(2-(3-amioo-6-methyIthseno[2,3-ft]pyridine-2-carboxam8do)ethyI) 99] Into a 50-mL round-bottom flask, was placed fert-butyl 4-[4-(2-aminoethyl)-2- fluorophenyl]piperazine-l -carboxylate (Example 44: Intermediate 44. 0.150 g, 0,46 mmol), HOBT (0.075 g, 0.56 mmol), EDCI (0.107 g, 0.56 mmol), 3-amino-6-methylthieno[2,3- b]pyridine-2-carboxyli.c acid (Example .1: Intermediate 1, 0, 106 g, 0,51 mmol), dichloromethane (10 mL), and DIEA (0.179 g, 0.247 mL, 1.39 mmol). The resulting solution was refluxed for 3 h in an oil bath and then cooled and concentrated in vacuo to afford a crade product that was purified by Prep-HPLC (Column, XBridge Prep RIM S, 5 μηι, 19* 150 mm; Mobile phase, A: water (contains 0.05% NH4OH) and B: CBbCN (60% to 70% over 6 min); UV Detector, 254 nm) to afford fert-butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-b]pyridine-2- carboxamido)ethyl)-2-fluorophenyl)piperazine-l-carboxylate as a yellow solid (42%). LCMS (i- SI. /?; .:)· 514 [M+Hf.
210
144628010 vl Step 2. 3-Amino-N-(3-fluoro-4-(piperazin-l-yl)phenethy
carboxamide (hydrochloride salt)
[00500] Into a 50-mL round-bottom flask, was added teri-butyl 4-(4-(2-(3-amino-6- methylthieno[2,3-b]pyridine-2-carboxamido)ethyl)-2-fluorophenyl)piperazine-l-carboxylate
(0,090 g, 0.17 mrnol) followed by dioxane (5 mL). Hydrogen chloride (gas) was bubbled through the solution for 30 seconds and the reaction was stirred for 2 h at RT. The resulting mixture was concentrated in vacuo to afford a solid that was collected by filtration, washed with ether and dried in vacuo to afford 3-amino-iV-(3-fluoro-4-(piperazin-l-yl)phenethyl)-6- methylthieno[2,3-0]pyridine-2-carboxamide (hydrochloride salt) as a yellow solid (22.6 mg, 29%). I . CMS (ESI, m/z): 414 [M+H] ;"; Ή NM (300 MHz, DMSO-di) δ ppm 8.32 (d, J = 8, 1 Hz, 1H), 7.32 (d, J= 8.4 Hz, 1H), 7.15 (t, J= 8.7 Hz, 1H), 6.77-6.73 (m, 2H), 3.36-3,33 (m, 6H), 3.19-3.16 (m, 4H), 2.77-2.75 (m, 21 1 ). 2.50 (s, 3H),
Example 81-1: 3-Amino-N-(2,5-difiuoro-4-(piperazin-l-yi)phenethyI)-6-methylthieno[2,3- h\ py r idine-2-carboxaniide (I- 11)
Figure imgf000212_0001
Step 1. tert-Butyl 4-(4-(2-(3-amino-6-methylthieno [2,3-6] yridine-2-car boxaniido)ethyl)- 2,5-difluorophenyl)piperazine-l-carboxylate
[00501] Into a 500-mL round-bottom flask was added teri-butyl 4-(4-(2-aminoethyl)-2,5- difluorophenyl)piperazine-l-carboxylate (3.93 g, 11.5 mmol), 3-amino-6-methylthieno[2,3- Z»]pyridine-2-carboxylic acid (2.63 g, 12.7 mmol), HOBt (2.11 g, 13.8 mmol), EDCI (2.65 g, 13.8 mmol) and dichloromethane (115 mL). DIEA (6.03 mL, 34.5 mmol) added, and the resulting mixture was stirred for 2 h at 40 °C in an oil bath. The reaction was cooled to RT and
211
144628010 vl water (60 mL) was added. The layers were separated, and the aqueous layer was extracted with DCM (3 x 80 mL), The combined organic layers were concentrated in vacuo to provide a crude product that was dissolved in EtOAc (250 mL) and washed sequentially with aqueous NaHCC (10 mL of saturated solution was diluted with 10 mL of water), water, and then aqueous saturated sodium chloride. The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford the crude product. The material was dissolved in DCM (~ 60 mL; with heat) and purified by FCC eluting with DCM/EtOAc (5:2) to afford fert-butyl 4~(4- (2-(3-amino-6-methylthieno[2,3-0]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl) piperazine-l-carboxylate as a solid (4.3 g, 71%), LCMS (ESI, m/z) 532 [M+H] \
Step 2, 3-Amino-N-(2,5-difluoro-4-(piperazin-l-yI)phenethyI)-6-methylthieno[2,3- 6]pyridine-2-carboxamide
[00502] Into a 50-mL round-bottom flask was added fert-butyl 4-(4-(2-(3-amino-6- methylthieno[2,3-¾]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)piperazine-l-carboxylate (0.300 g, 0.56 mmol), dichloromethane (30 mL) and trifluoroacetic acid (2 mL) and the resulting solution was stirred for 1 h at RT, The reaction mixture was cooled with a water/ice bath and the pH of the solution was adjusted to approximately 8 with aqueous saturated sodium carbonate. The resulting solution was extracted with dichloromethane (2 x 20 mL) and then the combined organic layers were then dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 3-amino-N"-(2,5-difluoro-4-(piperazin-l -yl)phenethyl)-6-methylthieno[2,3- yridine~2-carboxamide as a white solid (213 mg, 67%). LCMS (ESI, m/z) 432 [M 1 1 ] . Ή NMR (300 MHz, DMSO-i¾) δ ppm 8.29 (d, J = 8.1 Hz , 1H), 7.74 (s, 1H), 7.29 (d, J = 8.1 Hz, 1 H), 7.08-7.19 (m, 3H), 6.76-6.82 (m, 1 H), 3.39-3.62 (m, 2H), 2.76-2.89 (m, 10H), 2.63 (s, 3H), 2.34-2.50 (m,lH).
[00503] The Examples in Table 14 below were synthesized according to the procedures outlined above for Example 81-1 (Ml), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appear below the examples.
Table 14:
212
144628010 vl
Figure imgf000214_0001
213
144628010 vl
Figure imgf000215_0001
Figure imgf000216_0001
Figure imgf000217_0001
216
144628010 vl
Figure imgf000218_0001
Figure imgf000219_0001
218
144628010 vl Example
min); UV Detector: 254 nm. After the fractions containing product were combined and lyophilized the resulting solid was dissolved in MeOH (2 mL) and free-based using a resin-bound HCO3 cartridge (PL- HCO3 MP SPE, 500 mg per 6 mL tube).
J Notes: 4 N HCl in dioxane was used in Step 2, Prep HPLC Purification Method: (Water I):
Column: Xbridge C18, 5 μηι, 19* 150 mm; mobile phase: A: water (containing lOmM NH4HCO3 + 0,05% ammonium hydroxide) and B: CH3CN (15% to 40% over 8 min and then 45% to 80% over 8 min); UV Detector: 254 nm.
4 N otes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1.
TFA/DCM was used in Step 2. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 μηι; mobile phase: A: water (containing lOmM NH4HCO3 + 0.05% ammonium hydroxide) and B: CH3CN (15% to 30% over 8 min); Flow rate: 20 mL/min; UV Detector: 254 nm.
5 Notes: Step 1 was conducted at RT, TFA/DCM was used in Step 2. Prep HPLC Purification Method: ((Waters -2767)): Column: XBridge RP18, 5 μτη, 19* 100 mm ; mobile phase: A: water (containing 0.03% ammonium hydroxide) and B: CH3CN (45% to 60% over 5 min); UV Detector: 254 nm.
6 Notes: Step 1 was conducted at RT. 4 N HQ in dioxane was used in Step 2 and MeOH was used as a cosoivent. The product was isolated as the HCl salt by filtration.
7 Notes: 4 N HCl in dioxane was used in Step 2 and MeOH was used as a cosoivent. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 μηι; mobile phase: A: water (containing 10 mM NH4HCO3 + 0,05% ammonium hydroxide) and B: C¾CN (15% to 45% over 8 min); UV Detector: 254 nm.
8 Notes: 4N HCl in dioxane was used in Step 2. The product as isolated as the HCl salt by concentration in vacuo, trituration of the resulting solid with ether, and filtration to isolate the solid product.
9 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1.
TFA/DCM was used in Step 2. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 um; mobile phase: A: water (contains 0.05% ammonium hydroxide) and B: CH3C (30% to 70% over 10 min); Flow rate: 20 mL/min; UV Detector: 254 nm.
10 Notes: HATU was used instead of EDCI HOBt, and DMF was used as the solvent in Step 1.
TFA/DCM was used in Step 2. Prep HPLC Purification Method: (Waters I): Column: XBridge C 18, 5 μτη; mobile phase: A: water (containing 0.05% ammonium hydroxide) and B: CH3CN (20% to 76% over 6 min): Flow rate: 15 mL/min; UV Detector: 254 nm.
n Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1.
TFA/DCM was used in Step 2. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 μιη; mobile phase: A: water (containing 0.05% ammonium hydroxide) and B: CH3CN (20% to 84% over 6 min); Flow rate: 1 mL/min; UV Detector: 254 nm.
12 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). 4 N HCl in dioxane was used in Step 2, Prep HPLC Purification Method: (waters 2767): Column: Waters XBridge C I 8 19* 150 mm, 5 μτη; mobile phase: A: water (containing 0.05% Formic Acid) and B: CH3CN: UV Detector: 254 nm.
13 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). TFA/DCM was used in Step 2. Prep HPLC Purification Method: (waters 2767): Column: X Bridge C 18, 19* 150 mm, 5 um; mobile phase: A: water (containing lOmM NH4HCO3 + 0.05% ammonium hydroxide) and B: CH3CN (30% to 75% over 8 min); UV Detector: 254 nm.
14 Notes: Step 1 was conducted at RT, Only Step 1 was conducted since deprotection step was unnecessary. Prep HPLC Purification Method: (Waters 2767): Column: X Bridge C 18, 19* 150 mm,
219
144628010 vl Example
5 μπι; mobile phase, A: Water (containing lOmM NHLtHCOs + 0.05%ammonium hydroxide) and B: MeCN (35% to 40% over 8 mm); UV Detector: 254 nm.
iS Notes: Step 1 was conducted at RT. Only Step 1 was conducted since deprotection was unnecessary. Prep HPLC Purification Method: (Waters 2767): Column: XBridge RP18, 5 um, 39 x 100 mm; mobile phase, A: water( ) niM NH4HCO3 + 0,05% ammonium hydroxide) and B: CH3CN (35% to 40% over 8 rain): UV Detector: 254 nm.
lc Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). 4 N HCl in dioxane was used in Step 2. Prep HPLC Purification Method: (Waters 1): Column: Xbridge Prep C18 OBD column, 5 m, 19* 150 mm; mobile phase: A: water (containing 0.03%
H4OH) and B: CH3CN (16% to 34% over 10 mm); UV Detector: 220 & 254 nm.
' ' Notes: 4 N HCl in dioxane was used in Step 2 and MeOH was used as a cosolvent. The product was isolated as the HCl salt by concentration of the reaction mixture to a slurry in vacuo, and filtration (washing the filter cake with diethyl ether) to isolate the solid product.
18 Notes: 4 N HCl in dioxane was used in Step 2 and MeOH was used as a cosolvent. The product was isolated as the HCl salt by concentration of the reaction mixture to a slurry in vacuo, and filtration (washing the filter cake with diethyl ether) to isolate the solid product.
19 Notes: 4 N HCl in dioxane was used in Step 2 and MeOH was used as a cosolvent. The product was isolated as the HCl salt by concentration of the reaction mixture to a slurry in vacuo and then filtered (washing the filter cake with diethyl ether) and purified further by mass-triggered reverse phase HPLC zv Notes: 4 N HCl in dioxane was used in Step 2. Prep HPLC Purification Method: (waters-2767): Column: X Bridge C18, 19* 150 mm, 5 um; mobile phase: A: water (containing 10 mM NH4HCO3 + 0.05% ammonium hydroxide) and B: MeCN (35% to 55% over 8 min); UV Detector: 254 nm.
21 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). 4 N HCl in dioxane was used in Step 2. Prep HPLC Purification Method: Column: X Bridge C I 8, 5 μ,ηι, 19 x 100 mm; Mobile phase: A: water (containing lOmM NH4HCO3 + 0.05% ammonium, hydroxide) and B: CH3CN (40% to 45% over 5 mm); Flow rate: 15 mL/min; U V Detector: 254 nm. 42 Notes: Step 1 was conducted at RT. 4 N HCl in dioxane was used in Step 2 and MeOH was used as a cosolvent. Purification conditions: The final product was isolated as the free base following aqueous workup with EA and saturated aqueous NaHCC .
23 Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column :X
Bridge C I 8, 19* 150 mm. 5 μηι; mobile phase: water (containing !OmM NH4HCO3 + 0.05% ammonia) and CH3CN; Gradient: 35% to 45% in 8 min; Flow rate: 15 mL/min; Detector, 254 nm.
Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 μιη; mobile phase: A: water (containing 10 mM NH4HCO3 + 0.05% ammonium) and B: CH3CN (15% to 45% over 4 min and then 45% to 75% over 6 min); Flow rate: 15 mL/min; UV Detector: 254 nm.
25 Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column:
SunFire Prep C18, 19* 150 mm, 5 μηι; mobile phase: A: water (containing 0.05% formic acid) and B: CH3CN (35% to 40% over 9 min); Flow rate: 15 mL/min, UV Detector: 254 nm; The purified product was dissolved in small amount of MeOH and passed through a pre-packed cartridge (agilent, PL-HCO3 MP SPE 100 mg/6 mL) to remove formic acid.
26 Notes: Step 2 was conducted with TFA DCM. Prep HPLC Purification Method: Column: SunFire Prep Phenyl, 19* 150 mm, 5 um: mobile phase: A: water (containing 0.05% ammonium hydroxide) and B: CH3CN (20% to 55% over 6 mm); Flow rate: 15 mL/min; UV Detector: 254 nm.
220
144628010 vl Example
11 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column: X Bridge CI 8, 19* 150 mm, 5 μπι; mobile phase: A: water (containing 10 mM NH4HCO3 + 0.05% ammonium hydroxide)and B: CH3CN (20% to 45% over 8 min); Flow rate: 20 mL/rnin; UV Detector: 254 nm. 8 Notes: HATU was used instead of EDCI HOBt, and DMF was used as the solvent in Step 1 (at RT). Step 2 was conducted with 4 N HCl/dioxane. Prep HPLC Purification Method: (Waters I): Column: X Bridge C 18, 19* 150 mm, 5 μιη; mobile phase: A: water (containing iOniM NH4HCO3 + 0.05% ammonium hydroxide) and B: CH3CN (30% to 60% over 8 min); Flow rate: 15 mL/min; UV Detector: 254 nm.
29 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). Step 2 was conducted with 4 N HCl/dioxane. Prep HPLC Purification Method: (Waters-2767): Column: X Bridge C 18, 19* 150nm, 5 μιη; mobile phase: A:Water (containing lOmM NHLtHQI + 0.05% ammonium hydroxide) and B: CH3CN (40% to 75% over 5 min); Flo rate: 15mL/min; UV Detector: 254 nm.
J° Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 μτη; mobile phase: A: water (containing 10 mM NH4HCO3 and 0.05% ammonium hydroxide) and B: CH3CN (48% to 58% over 6 min); UV Detector: 254 nm.
51 Notes: HATU was used instead of EDCI/HOBt, and DMF was used as the solvent in Step 1 (at RT). Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column: X Bridge C I 8, 19* 150 mm, 5 μπι; mobile phase: A: water (containing 10 mM NHtHCOs + 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 5 min, and then 45% to 75% over 5 min): Flow rate: 15 mL/min; UV Detector: 254 nm.
32 Notes: HATU was used instead of EDCI/HOBt, and THF was used as the solvent in Step 3 (at RT). Step 2 was conducted with 4 N HCl/dioxane. Prep HPLC Purification Method: Column, X Bridge C I 8, 19* 150 mm, 5 μηι; mobile phase, A: water (containing 10 mM NH(HC03 and 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 8 min, and then 45% to 85% over 8 min); Flow rate: 15 mi/miii; UV Detector: 254 nm.
3 Notes: Step 2 was conducted with 4 N HCl/dioxane. The product was isolated by direct isolation of the HC1 salt by filtration and washing the filter cake with diethyl ether.
Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column, X Bridge C18, 19* 150 mm, 5 μιη; mobile phase, A: water (containing 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 4min, and then 45% to 75% over 6 min); Flow rate: 15 mL/min; UV Detector: 254 nm.
35 otes: Step 2 was conducted with 4 N HCl/dioxane. Prep HPLC Purification Method: Column: X Bridge C18, 19* 150 mm, 5 um; mobile phase, A: water (containing 10 mM NH4HCO3 + 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 5 min, and then 45% to 75% over 5 min); Flow rate: 15 mL/min; UV Detector: 254 nm.
J0 Notes: Step 2 was conducted with 4 N HCl/dioxane with MeOH as a cosolvent. The product was isolated as the HC1 salt after direct filtration and washing the filter cake with diethyl ether.
3 Notes: Step 2 was conducted with 4N HCl/dioxane with MeOH as a cosolvent. The product was isolated as the HCI salt after direct filtration and washing the filter cake with diethyl ether.
221
144628010 vl Example
Notes: Step 2 was conducted with TFA/DCM. Prep HPLC Purification Method: Column, X Bridge C18, 19* 150 mm, 5 μηι; mobile phase, A: water (containing 10 mM M i ll ICO-, and 0.05% ammonium hydroxide) and B: CH3CN (28% to 38% over 3min, and then 38% to 75% over 6 min); UV Detector: 254 nm.
"" Notes: The individual enantiomers were separated after Step 1 using the following Chiral HPLC Method: (SH1MADZU LC-20AD): Column, DAICEL CHIRALPAK AD-3; mobile phase, Phase A: n- Hexane (containing 0.1% DBA) Phase B: Ethanol; UV Detector: 190mn - 500nm; Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: Column, X Bridge C18, 39* 150 mm, 5 μτη; mobile phase, A: water (containing 10 mM NH4HCO3 and 0.05% ammonium hydroxide) and B: CH3CN (28% to 38% over 3 mm; and th n 38% to 75% over 6 min); UV Detector: 254 nm,
4υ Notes: Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: Column, X Bridge C18, 19* 150 mm, 5 um; mobile phase, A: water (containing 10 mM NH4HCO3 and 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 5 min); LTV Detector: 254 nm.
41 Notes: Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (Waters-2767): Column, X Bridge C 18, 19* 150 mm, 5 μ,ηι; mobile phase. A: water (containing 10 mM NH4HCO3 and 0.05% ammonium hydroxide) and B: CH3CN (15% to 45% over 5 min, and then 45% to 75 % over 5 min); Flow rate: 20 mL/min); UV Detector: 254 nm.
42 Notes: Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (Waters-2767): Column, X Bridge C 1 , 1 * 150 mm, 5 μηι; mobile phase, A: water (containing 10 mM NH4HCO3 and 0,05% ammonium hydroxide) and B: CH3CN (15% to 45% over 5 min, and then 45% to 75 % over 5 min); Flow rate: 20 mL/min); UV Detector: 254 nm.
43 Notes: Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (Water I): Column, X Bridge C 18, 19* 150 nm, 5 μπι; mobile phase, A: water(containing lOmM NH4HCO3 and 0,05% ammonium hydroxide) and B: CH3CN (20% to 30% over 5 min); Flow rate: 20 mL/min; UV Detector: 254 nm.
4 Notes: Step 2 was conducted with 4 N HCl/dioxane and MeOH as a cosolvent. The product was isolated directly as the HQ salt by filtration and washing with diethyl ether.
43 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases in DMF solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (C18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
4 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases in DMF solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (C 18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
4 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2, was unnecessary. Purification Method: FCC eluting with 40 - 60% EtOAc in hexanes.
48 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was unnecessary-. Purification Method: FCC eluting with 40 - 60% EtOAc in hexanes.
144628010 vl Example
Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (C18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
50 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (C18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
51 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (C18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
52 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (CI column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
5j Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (€18 column: gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
54 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (CI 8 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
53 Notes: Step 1 was conducted using HBTU as the coupling reagent, DIEA and DMAP as bases, and DMF as the solvent at 80 °C. Step 2 was conducted with TFA/DCM. HPLC Prep Purification Method: (CT8 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes); the product was isolated by lyophilization.
i0 HPLC Prep Purification Method: Column, X Bridge C18; 19* 150 mm, 5 μηι; mobile phase: A: water (modified with 10 mM H4HCO;5 and 0.05% ammonia) and B: CH3CN; Gradient: 15% to 30% B in 8 rain; Flow rate: 20 mL/min.
57 Notes: TFA/DCM was used for Step 2. HPLC Prep Purification Method: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 30.0%, End Cone, of Pump B: 55 ,0%, Total Flow: 20 mL min, Time: 7 mm, Phase A: Water (10 mmol/L NH4HCO3), Phase B:
MeCN-HPLC, Column Name: SunFire Prep CIS OBD Column, Length: 150 mm. Internal Diameter: 19 mm. Particle Size: 5 μηι, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm.
Λ8 Notes: TFA/DCM was used for Step 2. HPLC Prep Purification Method: Waters, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 25.0%, End Cone, of Pump B: 35.0% Total Flow: 20 mL/min, Time: 10 mm, Phase A: Water (10 mmol/L NH4HC03 + 0,05% NH3H20), Phase B: MeCN-HPLC, Column Name: XBridge Prep CT8 OBD Column, Length: 150 mm, Internal
Diameter: 19 mm, Particle Size: 5 μηι, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-
223
144628010 vl
Figure imgf000225_0001
224
144628010 vl Example 82 (1-73): 3-Amino-A7-(3-(difluoroinethoxy)-4-(piperazin-l-yI)phenethyi)-6- methyIthieno[2,3-6]pyridine-2-carboxamide (formate salt)
Figure imgf000226_0001
Step 1. tert-Butyl 4-(4-(2-(2-chloroacetamido)ethyl)-2-(difluoromethoxy)phenyl)piperazine- l-carboxylate
[00504] Into a 50-mL round-bottom flask was added fert-butyi 4-[4-(2-aminoethyl)-2- (difluororaethoxy)phenyl]piperazine-l-carboxylate (0 210 g, 0,57 mmol), triethylamine (0. 172 g, 0.237 mL, 1.70 mmol) and dichloromethane (10 mL) followed by a solution of 2-chloroacetyl chloride (0.069 g, 0.61 mmol) in dichloromethane (2 mL). The resulting solution was stirred for 1 h at RT and then concentrated in vacuo. The resulting crude product was purified by FCC e!uting with ethyl acetate/petroleum ether (1 :1) to afford fer/-butyl 4-(4-(2~(2~ chloroacetamido)ethyl)-2-(difluoromethoxy)phenyl)piperazine-l-carboxylate as a white solid (220 nig. 87%). LCMS (ESI, m/z): 448 [M+Fff .
Step 2. t rt-llxii l 4-(4-(2-(3-amino-6-methyIthieno[2,3-^]pyi'idine-2-carboxainido)ethyl)-2- (difluoromethoxy)phenyl)piperazine-l-carboxylate
[00505] Into a 50-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added teri-buty] 4-(4-(2-(2-chloroacetamido)ethyl)-2- (difluoromethoxy)phenyl)piperazine-l-carboxylate (0.220 g, 0.49 mmol), 2-mercapto-6- methylnicotinonitrile (0.110 g, 0.73 mmol), potassium carbonate (0.204 g, 1.48 mmol), and DMF (4 mL). The reaction mixture was stirred overnight at 80 °C and then quenched with water (20 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified by FCC
225
144628010 vl eluting with ethyl acetate/petroleum ether (1 : 1) to afford tert-b yi 4-(4-(2-(3-amino-6- methylthieno[2,3-Z»]pyridine-2-carboxamido)ethyl)-2-(difluoromethoxy)phenyl)p
carboxylate as a red solid (300 rag, 99%), LCMS (ESI, m/z): 562 j \! H i .
Step 3. 3-Amino-N-(3-(difluoromethoxy)-4-(piperazin-l-yI)phenethyl)-6-methylthieno[2,3- A]pyridine-2-carboxamide (formate salt)
[00506] Into a 50-raL round-bottom flask was added fe -butyl 4-(4-(2-(3-amino-6- methylthieno[2,3-£]pyridine-2-carboxamido)eth^^
carboxylate (0.290 g, 0.52 mmol) and 4 N hydrogen chloride in 1,4-dioxane (20 raL). The reaction mixture was stirred for 2 h at RT and then concentrated in vacuo to provide a crude product that was purified by Prep-HPLC under the following conditions (waters-2767): Column: SunFireTM Prep CI 8, 5 μηι, 19 x 150 mm; mobile phase, A: water (containing 0.1% formic acid) and B: CH3CN (20% to 25% over 3 min); UV Detector: 220nm. This afforded the title compound as a light yellow solid (4 mg, 1%). LCMS (ESI, m/z): 462 [M+Hf; ' i ! -NMR (300 MHz, DMSO-de) δ ppm 8.36-8.21 (ra, 2H), 7.77-7.69 (m, 1H), 7,31-7,29 (ra, 11 1 ). 7.12-6.78 (m, 5H), 3.40-3.37 (m, 2H), 2.89 (s, 8H), 2.80-2.77 (m, 2H), 2.62 (s, 3H).
Example 83 (1-74); 3-Amino-6-methyl-N-(3-methyl-4-(piperazin-l-yl)phenethyl)thieno [2,3- h] py r idine-2-carboxam ide
Figure imgf000227_0001
¾reP * Example 83 (1-74)
Step J. Benzyl 4-(4-(2-(2-chloroacetamido)ethyl)-2-methylphenyl)piperazine-l-carboxylate
[00507] Into a 50-mL round-bottom flask was added benzyl 4-[4-(2-aminoethyl)-2- methylphenyl]piperazine-l-carboxylate hydrochloride (0.372 g, 0.94 mmol), Et M (0.291 g, 0.401 mL, 2.88 mmol), and dichloromethane (20 mL). This was followed by the dropwise addition of a solution of 2-chloroacetyl chloride (0, 128 g, 0.090 mL, 1.13 mmol) in
226
144628010 vl dichloromethane (5 mL) at 0 °C with stirring. The resulting solution was stirred for 2 h at 0 °C and then concentrated in vacuo to a crude product thai was purified by FCC eluting with ethyl acetate/petroleum ether (1 :3) to afford benzyl 4-(4-(2-(2-chloroacetamido)ethyl)-2- methylphenyl)piperazine-l-carboxylate as a white solid (272 mg, 67%). LCMS (ESI, m/z): 430 1 \i i i I
Step 2. Benzyl 4-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- methyIphenyl)piperazine-l-carboxylate
[00508] Into a 50-mL round-bottom flask was added benzyl 4-(4-(2-(2- chloroacetamido)ethyl)-2-methylphenyl)piperazine-l -carboxylate (0.27 g, 0.63 mmol), 2- mercapto-6-methylnicotinonitrile (0.1 14 g, 0.76 mmol), potassium carbonate (0.261 g, 1.89 mmol) and DMF (4 mL). The reaction mixture was heated and stirred overnight at 80 °C and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1 ) to afford benzyl 4-(4-(2-(3-amino-6-methyithieno[2,3-^]pyridine-2- carboxamido)ethyl)-2-methylphenyl)piperazine-l-carboxylate as an orange oil (212 mg, 62%). LCMS (ESI, m/z): 544 [M+H]+.
Step 3. 3-Amino-6-methyl-N-(3-methyl-4-(piperazin-l-yl)phenethyl)thieno[2,3-6]pyridine- 2-carboxamide
[00509] Into a 50-mL round-bottom flask was added benzyl 4-(4-(2-(3-amino-6- methylthieno[2,3-b]pyridine-2-carboxamido)ethyl)-2-methylphenyl)piperazine-l-carboxylate
(0.200 g, 0.37 mmol) and dichloromethane (1 mL). The resulting mixture was cooled to -30 °C and then a solution of BBn in DCM was added dropwise (1 M; 2 mL). The resulting solution was stirred for 30 rnin at -30 °C and then concentrated in vacuo to provide a cmde product that was purified by Prep-HPLC using the following conditions (waters-2767): Column, SunFirelM CI 8, 5 μΐΉ, 19* 150 mm; mobile phase, A: water (containing 0.05% ammonia) and B: CFbCN (18% to 25% over 8 min); UV Detector: 254 nm. This afforded the title compound as a light yellow solid (6.5 mg, 4%), LCMS (ESI, m/z): 410 j \! H j \: :! ! \!R (300 MI !/, DMSO-fife) δ ppm 8.30 (d, ./ = 8.4 Hz, 1H), 7.79 - 7.62 (m, 1H), 7.31 (d, J= 8.0 Hz, 1H), 7.12 (br s, 2H), 7.03 - 6.88 (m, 3H), 3.41 - 3 ,33 (m, 2H), 2.88 - 2.79 (m. 4H), 2,75 - 2,66 (m, 6H), 2.58 (s, 3H), 2.22 (s, 3! !).
144628010 vl [00510] The Examples in Table 15 below were synthesized according to the procedures outlined above for Examples 82 (1-73) and 83 (1-74), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appears below each example. Method A (Acid promoted amine deproteciion) or Method B (BBo promoted amine deprotection) was used in Step 3.
Table 15:
Figure imgf000229_0001
228
144628010 vl
Figure imgf000230_0001
229
144628010 vl
Figure imgf000231_0001
230
144628010 vl
Figure imgf000232_0001
231
144628010 vl
Figure imgf000233_0001
232
144628010 vl
Figure imgf000234_0001
233
144628010 vl Examples 85-A (1-91) and 85-B (1-92): cis-3-Amino-6-methyl-N-(3-(4-( 1 yl)phenyl)cyclobutyl)thieno [2,3-6] pyridine-2-carboxamide (formate salt) and
amino-6-methyI-N-(3-(4-(piperazin-]-yl)phenyi)cyclobutyI)thieno[2,3-6]pyridine-2'
Figure imgf000235_0001
Figure imgf000235_0002
Step 6 Step 7
Figure imgf000235_0003
xamp e - - )
Step J. 3-(4-Bromophenyl)-2,2-dichlorocyclobutan-l-one
[00511] Into a !GO-mL round-bottom flask was added 1 -bromo-4-ethenylbenzene (0 920 g, 5.03 mmol) and ether (30 niL) followed by the dropwise addition of POCb (0.51 niL) with stirring. Trichloroacetyl chloride (1.84 g, 10.12 mmol) and Zn-Cu (0.980 g, 15.1 mmol ) were then added portion-wise and the reaction mixture was stirred for 2 h at 40 °C. The reaction was cooled to RT and allowed to stir for an additional 16 h. The solids were reraoved by filtration and the filtrate was washed with brine (50 n L), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford 3-(4-bromophenyl)-2,2-dichlorocyclobutan-l-one as a white solid (400 mg, 27%). LCMS (ESI, m/z): 293, 295 | M l i s .
234
144628010 vl Step 2. 3-(4-Bromophenyl)cyclobutan-l-one
[00512] Into a 500-mL round-bottom flask was added 3-(4-bromophenyl)-2,2- dichlorocyclobutari-l-one (13.9 g, 47,4 mmol), zinc (12,6 g, 190 mmol), and acetic acid (200 mL). The reaction mixture was stirred for 2 h at RT and then heated to 120 °C and stirred for an additional 6 h. The reaction mixture was filtered, and the filtrate was diluted with H2O (200 mL) and extracted with dichlorom ethane (3 x 200 mL). The combined organic layers were washed with 5% aqueous sodium bicarbonate (200 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified by FCC eluti ng with ethyl acetate/petroleum ether (1 :2) to afford 3-(4-bromophenyl)cyclobutan-l-one as a light yellow solid (7.8 g, 73%), LC S (ESI, m/z): 225, 227 | \ ! H i
Step 3. N-benzyl-3-(4-broraopheiiy1)cycIobutaii-l-aiiii5ie
[00513] Into a 250-mL round-bottom flask was added 3-(4-bromophenyl)cyclobutan-l-one (3.00 g, 13.3 mmol) and benzylamine (1.40 g, 13.1 mmol) followed by the addition of a solution of 1 i{{)/-Pr) ; ( 15.0 g, 16.0 mmol, 52.8 mmol) in ethanol (120 mL) at 50 °C. The resulting solution was stirred for 6 h at 50 °C and then cooled to RT. NaBFfeCN (1.20g, 19.1 mmol) was added and the reaction mixture was allowed to stir for an additional 12 h. The reaction mixture was concentrated in vacuo and the crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :4) to afford N-benzyl-3-(4-bromophenyl) cyclobutan-1. -amine as a yellow liquid (2.2 g, 52%). LCMS (ESI, m/z): 316, 318 [M+H]÷
Step 4, 4-(4-(3-(benzylamino)cyclobutyl)phenyl)piperazine-l-carboxylate
[00514] Into a 25-mL round-bottom flask was added A-benzyl-3-(4-bromophenyl)cyclobutan- 1 -amine (0.100 g, 0.31 mmol), ferf-butyl piperazine-l-carboxylate (0.059 g, 0.32 mmol), Pd(dppf)Ch (0.023 g, 0.03 mmol), SPhos (0.013 g, 0.03 mmol), and CS2CO3 (0,300 g, 0.95 mmol) followed by toluene (8 mL). Nitrogen was bubbled through the reaction mixture for 5 minutes and then the reaction mixture was stirred for 12 h at 100 °C. After cooling to RT, the reaction mixture was concentrated in vacuo and the crade product was purified by FCC eluting with ethyl acetate/petroleum ether (0 to 100%) to afford fert-butyl 4-(4-(3- (benzylamino)cyclobutyl)phenyl) piperazine-l-carboxylate as a yellow solid (58 mg, 44%). LCMS (ESI, m/z) 422 [M+H]+.
235
144628010 vl Step 5. tert~Buty\ 4-(4-(3-amiiiocycIol)iityI)p eiiyI)piperazi!ie-l-car oxy!ate
[00515] Into a 250-mL pressure reactor under an atmophere of nitrogen was added a solution of &T/-butyl 4-(4-(3-(benzylamino)cyclobutyl)phenyI)piperazine-l-carboxylate (1.10 g, 2.61 mmol) in methanol (100 mL) followed by the addition of 10% palladium on carbon (0.027 g, 0,25 mmol). The reaction vessel was pressurized with hydrogen (10 atm) and the resulting mixture was stirred for 12 h at 60 °C. The reaction was vented to nitrogen, the solids were removed by filtration through a pad of Celite and the resulting filtrate was concentrated in vacuo to afford tert-butyl 4-(4-(3-aminocyclobutyl)phenyl) piperazine-l-carboxylate as a gray solid (800 mg, 93%). LCMS (ESI, m/z): 332 | M ! i j .
Step 6, tert-Bntyl 4-(4-(3-(2-chloroacetamido)cyclobutyl)phenyl)piperazine-l-carboxylate
[00516] Into a 25-mL round-bottom flask was added tert-butyl 4-(4-(3- aminocyclobutyl)phenyl)piperazine-l-carboxylate (0.400 g, 1.21 mmol) followed by tetrahydrofuran (5 mL), 2-Chloroacetyl chloride (0.136 g, 1.20 mmol), and DIEA (0.310 g, 0.418 mL, 2.40 mmol). The resulting solution was stirred for 2 h at RT and then concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :1). This afforded the title compound as a gray solid (240 mg, 49%), LCMS (ESI, m/z): 408 [ \i l f |
Step 7. fert-Butyl 4~(4-(3-(3~affliiio~6~methyIihieno[2,3~ i]pyridiiie~2~carboxaiiiido) cycloboiyi)p e!iyl)piperazine-l-carboxylaie
[00517] Into a 25-mL round-bottom flask was added ferf-butyi 4-(4-(3-(2- chloroacetamido)cyclobutyI)phenyl)piperaziiie-l-carboxylate (0.085 mg, 0.21 mmol) followed by THF (5 mL). 2-mercapto-6-methylnicotinonitrile (0.047 g, 0.31 mmol,) was added followed by sodium meth oxide (0.034 g, 0.63 mmol). The resulting solution was stirred for 14 h at 50 °C and then concentrated in vacuo. The cmde product was purified by FCC eluting with ethyl acetate/petroleum ether (1 : 1) to afford tert-butyl 4-(4-(3-(3-amino-6-methylthieno[2,3- ^]pyridine-2-carboxamido)cyclobutyl)phenyl)piperazine-l -carboxylate as a yellow solid (45 mg, 41%). LCMS (ESI, m/z): 522 [ M i l ]
236
144628010 vl Step 8. cis-3-Amino-6-methyI-N-(3-(4-(piperazin-l-yl)phenyl)cyclobutyl)thieiio[2,3- 6]pyridine-2-carboxamide (formate salt) and ir«M~3~afflino-6~niethyI ' /~(3~(4-(piperazin~l~ yl)phenyI)cyclobutyl)thieno[2,3-6]pyridme-2-carboxamide (formate salt)
[00518] Into a 25-mL round-bottom flask was added fert-butyl 4-(4-(3-(3-amino-6- methylthieno[2,3-0]pyridine-2-carboxamido)cyclobutyl)phenyl)piperazine-l -carboxylate (0.050 g, 0.05 mmoi) followed by dichloromethane (5 mL) and TFA (0.5 mL). The resulting solution was stirred for 1 h at RT and then concentrated in vacuo. The crude product was diluted with DMF (2 mL) and then purified by Prep-HPLC using the following conditions (WATERS 2767): Column, Column: X Bridge CI 8, 19* 150 mm, 5 μηι; mobile phase, mobile phase, A: water (containing 0.1% FA)and B: CH3CN; Detector, 254 nm, UV wavelength: 254 nm to afford cis-3- amino-6-methyl-A/-(3-(4-(piperazin^
(formate salt) (Example 85-A (1-91), 12.3 nig, 30%) as a light yellow solid, and 12 mg of the cisl trans isomer mixture. The cisl trans isomer mixture (12 mg) was purified further by Chiral- Prep-HPLC using the following conditions (SHIMADZU i .C-20AD): Column, DAICEL CHIRA CEL OJ-3; mobile phase, A: Ethanol (containing 0.1% DEA) and B: Methanol; UV Detector: 190 nm to 500 nm to afford 2rfirws,-3-amino-6-methyl-A'-(3-(4-(piperazin-l-yl)phenyl) cyclobutyl)thieno[2,3-i]pyridine-2-carboxamide (Example 85~B (1-92), 2.2 mg, 5%) as a light yellow solid.
[00519] Example 85-A (1-91): LCMS (ESI, m/z): 422 [M+LTf; ¾ NMR (300 MHz, CD3OD) δ ppm 8.20 (d, J = 8.4 Hz, IH), 7,25- 7,30 (m, 3H), 7,98- 6,04 (m, 2H), 4,40-4,52 (m, I H), 3.39-3.22 (m, 8H), 3.20-3. 10 (m, 11 1 )., 2.65-2.77 (m, 2H), 2.63 (s, 3H), 2.20-2.26 (ni, 21 1 ».
[00520] Example 85-B (1-92); LCMS (ESI, m/z): 422 [M+H]+; !H NMR (300 MHz, CD3OD) δ ppm 8,24 (d. ./ - 8.4 Hz, IH), 7.30- 7.35 (m, 3H), 7.02- 7.05 (m, 2H), 4.60-4.71 (m, IH) , 3.49-3.63 (m, IH), 3.40 (br s, 8H), 2.67 (s, 31 1). 2.63-2.42 (m, 4H).
237
144628010 vl Examples 86- 1A (1-93) and 86-1 B (1-9· i): 3-Amino-N-(4-((3a>S,6a$)-hexahydropyrrolo[3,2- 6] pyrrol- l(2/ )-yl)phenethyl)-6-methylt tiieno [2,3-6] pyridine-2-carboxamide (hydrochloride salt) (stereochemical configuratio n assumed) and 3-Ammo-/V~(<
hexahydropyrrolo [3,2-6] p rrol- 1 (2Η)-γ l)phenethyl)-6-methylthieno [2,3-6] pyridine- carboxamide (hydrochloride salt) (stereochemical configuration assumed)
Figure imgf000239_0001
CH2Ci2, reflux
Step 1 Chiral HPLC Separation
Figure imgf000239_0002
Enantiomer A Ersaniiomer B
HC!/dioxane HCi/dioxane
MeOH MeOH
2
Figure imgf000239_0003
Example 86-1A (1-93) Example 86-1 B (1-94)
Step 1. tert-Butyl (3at?,6aiS<)-4~(4-(2-(3-amisio-6-metbylt!iieiio[2,3-/>]pyridi!ie-2- carboxamido)ethyl)pheoyl)hexahydropyrrolo[3,2-i»]pyrrole~l(2i: )~carboxylate
(stereochemical configuration assumed) and ferf-Butyl (3a ?,6a/f)-4-(4-(2-(3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)phenyl)hexahydropyrrolo[3,2-6]pyrrole- l(2i )-carboxylate (stereochemical configuration assumed)
[00521] Into a 25-mL round-bottom flask was added fcrt-butyl 4-[4-(2-aminoethyl)phenyl]- octahydropyrrolo[3,2-6]pyrrole-l-carboxylate (0.300 g, 0.91 mmol), 3-amino-6-methylthieno [2,3-6]pyridine-2-carboxylic acid (0.188 g, 0,90 mmol), HOBt (0, 146 g, 1 .08 mmol), EDCI (0.208 g, 1.09 mmol), and dichloromethane (5 niL) followed by the addition of DIEA (0.350 mg, 0.472 niL, 2.71 mmol). The resulting solution was stirred for 2 h at reflux and then cooled and washed with water (2 x 10 mL). The organic layer was concentrated in vacuo to provide a crude product that was further purified by Prep-HPLC using the following conditions (Waters I):
238
144628010 vl Column, Xbridge phenyl column, 5 μιπ, 19*150 mm; mobile phase, A: water (0.05% NH4OH + 10 mM H4HCO3) and B: CH3CN (52% to 57% over 7 min); UV Detector: 254 nm. This afforded the title compounds as a mixture of cis stereoisomers (light yellow solid, 150 mg, 32%. The stereoisomers were then separated by Chiral-Prep-HPLC using the following conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50.0%, Total Flow: 1 mL/min, Column, DAICEL CHIRALCEL OD-3; mobile phase, Phase A: w-Hexane (0.1% DEA), Phase B: Ethanol : Length: 50 mm, Internal Diameter: 4.6 mm, Particle Size: 3.0 μηι Column Temp. : 25 °C, PDA Model: SPD-M20A, Wavelength: 190 nm to 500 nm. This resulted in the following: Step 1, Enantiomer A: 1st eluting peak: (retention time = 2.45 min, 40 mg, yellow solid), LCMS (ESI, m/z): 522 [M+H]T. Step 1, Enantiomer B: 2na eluting peak: (retention time = 4.34 min, 50 mg, yellow solid). LCMS (ESI, m/z) 522 [M+H]+.
Step 2. Example 86-1A (1-93); 3-Aniino-N-(4-((3a.S',6ai.S -hexahydropyrroIo[3,2-6]pyrrol- l(2i )-yl)phenethyl)-6-methyithienoi2,3-6]pyridine-2-carboxamide (hydrochloride salt) (stereochemical configuration assumed)
[00522] Into a 25-mL round-bottom flask was added fe -butyl (3aS,6aS {assumed))- -{A-^l- (3-amino-6-methylthieno[2,3-/>]pyridine-2-carboxaraido)ethyl)phenyi)h
pyrrole- 1 (2//)-carboxylate (40 mg, 0.08 mmol) and methanol (2 mL) followed by 4 N HCl/dioxane (2 mL) and the resulting solution was stirred for 2 h at RT. The reaction mixture was concentrated in vacuo to remove some of the solvent and the resulting solids were collected by filtration, washed with ether (2 x 10 mL), and dried in vacuo to afford the title compound as a yellow solid (5,6 mg, 16%). LCMS (ESI, m/z): 422 [M+H]+; 5 H-\'\ ] R (300 MHz, DMSO-t/-) δ ppm 9.56 (br s, 1H), 9.30 (br s, 1H), 8.34 (d, J - 8.3 Hz, 1H), 7.83 - 7.60 (in, IH), 7.32 (d, J = 8,3 Hz, 1 1 1 ),, 7.08 (d, ./ 8,4 Hz, 2H), 6.60 (d, ,/ 8,5 Hz, 2H), 4.32 (br s, 2H), 3.58 - 3.41 (m, IH), 3.41 - 3.13 (m, 4H), 3.06 (br s, IH), 2.78 - 2.64 (m, 2H), 2.55 (s, 3H), 2.31 - 2.16 (m, 2H), 2.16 - 2.05 (m, IH), 1.94 - 1.82 (m, IH).
Step 2. Example 86-1B (1-94): 3-Amino-A-(4-((3aK,6aR)-hexahydropyrroiol3,2-^]pyrrol- l(2H)-yl)phenethyl)-6-methylthieno[2,3-6]pyridine-2-carboxamide (hydrochloride salt)
(stereochemical configuration assumed)
[00523] The same synthetic procedure described to prepare Example 86-1A (1-93) was applied to Enantiomer B to afford the title compound as a yellow solid (17 mg, 39%). LCMS
239
144628010 vl (ESI, m/zy. 422 j XI ί I ) : ¾-NM (300 MHz, DMSiW- } δ ppm 9.51 (br s, i l l ). 9.26 (br s, 1H), 8.33 (d, J = 8.1 Hz, 1H), 7.72 - 7.68 (m, 1H), 7,32 (d, J = 8.4 Hz, I I I), 7.08 (d, J = 8.4 Hz, 2H), 6.60 (d, / 8.5 Hz, 21 i ). 4.32 (br s, 2H), 3.52 -- 3.44 (m, 1 H), 3 ,44 - 3.23 (m, 4H), 3,06 (br s, IH), 2.73 - 2.68 (m, 2f l). 2.51 (s, 3H), 2.31 ~- 2.06 (m, 3H), 1.94 - 1.87 (m, 1H).
[00524] The Examples in Table 16 below were synthesized according to the procedures outlined above for Examples 86- 1A (1-93) and 86-1 B (1-94), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as Chiral HPLC (after Step 1) and Prep HPLC purification conditions appear below the examples.
Table 16:
Figure imgf000241_0001
240
144628010 vl
Figure imgf000242_0001
241
144628010 vl
Figure imgf000243_0001
242
144628010 vl
Figure imgf000244_0001
243
144628010 vl
Figure imgf000245_0001
244
144628010 vl
Figure imgf000246_0001
245
144628010 vl
Figure imgf000247_0001
144628010 vl [M+H]
Notes: Step 2 was conducted with TFA/DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-2QAD, LC parameters: Pump Mode: Binary gradient. Start Cone, of Pump B: 35%, Total Flow: 20 mL min, Phase A: Hexane-HPLC, Phase B: EtOH-HPLC, Column Name: DAICEL CHIRALPAK IC, Length: 250 mm, interna] Diameter: 20 mm. Particle Size: 5 um, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1, Enantiomer A: lsl eluting peak (retention time = 9.20 min, 40 mg, 21%) as a yellow solid. LCMS (ESI, m/z): 564 [M+H]+ Step 1. Enantiomer B: 2,id eluting peak (retention time :::: 11.3 min, 40 nig, 21%) as a yellow solid. LCMS (ESI, m/z) 564 ! M i ! | . Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient. Start Cone, of Pump B: 5.0%, End Cone, of Pump B: 77.0%, Total Flow: 20 mL/min, Time: 7 min, Phase A: Water (10 mmol/L NH4HCO3), Phase B: MeCN-HPLC, Column Name:
\ Bridge Prep CIS OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μτη, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm
4 Notes: Step 1 was conducted at RT. Step 2 was conducted with TFA/DCM, Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50.0%, Total Flow: 20 m l . /min. Phase A: Hexane (0.1% DEA), Phase B: Ethanol- HPLC, Column Name: (R,R)-WHELK-01-Kromasii, Length: 250 mm, Internal Diameter: 50 mm, Panicle Size: 5 um. Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1 , Enantiomer A: 1st eluting peak (retention time ::= 6.3 min, 45 mg, 15%) as a white solid. LCMS (ESI, m/z): 576 [M+H]+. Step 1. Enantiomer B: 2nd eluting peak (retention time = 8.3 min, 45 mg, 15%) as a white solid. LCMS (ESI, m z): 576 [M+H]+. Prep HPLC Method for Step 2:
SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient Start Cone, of Pump B: 35.0%, End Cone, of Pump B: 45.0% Total Flow: 20 mL/min, Time: 8 mm, Phase A: Water (30 mmol/L NH4HCO3), Phase B: MeCN-HPLC, Column Name: XBridge Prep CT8 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μιη, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm.
5Notes: Step 2 was conducted with TFA/DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50%, Total Flow: 15 mL/min, Phase A: Hexane (0.1% DEA), Phase B: EtOH-HPLC, Column Name: DACEL
CHIRALPAK AD-H SFC, Length: 25 cm, Internal Diameter: 5 cm, Particle Size: 5 um. Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1, Enantiomer A: 1st eluting peak (retention time = 1 1.2 min, 40 mg, 53%) as a yellow solid. LCMS (ESI, m/z): 554 [M+H]". Step 1 , Enantiomer B: 2nd eluting peak (retention time = 14.0 min, 30 mg, 40%) as a yellow solid. LCMS (ESI, m/z): 554 [M+H]+. Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 12.0%, End Cone, of Pump B: 42.0% Total Flow: 20 mL/min, Time: 7 mm. Phase A: Water (10 mmol/L NH4HCO3), Phase B:
MeCN-HPLC, Column Name: XBridge Prep C18 OBD Column, Length: 150 mm. Internal Diameter: 19 mm, Particle Size: 5 \xm. Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm.
6 Notes: Step I was conducted at RT. Step 2 was conducted with TFA DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 30.0%, Total Flow: 20 mL/min, Phase A Hexane (0.1 %DEA), Phase B: Ethanol- HPLC, Column Name: DAICEL CHIRALPAK AD-H-SL001 , Length: 250 mm, Internal Diameter: 20 mm, Particle Size: 5 u . Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from. 390 nm to 500 nm . Step 1, Enantiomer A: 1st eluting peak (retention time = 11.2 min, 40 mg, 22%) as a yellow
144628010 vl [M+H]
oil. LCMS (ESI, m/z): 554 [M+Hf. Step 1, Enantiomer B: 2nu eluting peak (retention time :::: 14.8 mm, 30 mg, 16%) as a yellow oil. LCMS (ESI, m/z): 554 j N! - ! l j . Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 25.0%, End Cone, of Pump B: 65.0% Total Flow: 20 raL/min, Time: 7 min. Phase A: Water (10 mmol/L \i hi !( ():.}. Phase B: MeCN-HPLC, Column Name: XBndge Prep C18 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μιη, Aperture Size: 130 A, Column Temp; 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm.
7 Notes: Step 2 was conducted w th TFA/DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient. Start Cone, of Pump B: 30.0%, Total Flow: 20 mL/min, Phase A Hexane (0.1% DEA), Phase B: IPA-HPLC, Column Name: DAICEL
CHJEALPAK-AD-H-SLOOl, Length: 250 mm, internal Diameter: 20 mm. Particle Size: 5 μιη, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1, Enantiomer A: lsi eluting peak (retention time = 8.2 min, 40 mg, 21%) as a yellow solid. LCMS (ESi, /z) 572 [M+H]". Step 1, Enantiomer B: 2nd eluting peak (retention time = 12.0 min, 40 mg, 21%) as a yellow solid, LCMS (ESI, m/z): 572 [M+Hf. Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 5.0%, End Cone, of Pump B: 69.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (10 mmol/L NH4HCO3), Phase B: MeCN- HPLC, Column Name: XBndge Prep C18 OBD Column, Length: 150 mm, internal Diameter: 19 mm. Particle Size: 5 μτη, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A,
Wavelength: from 190 nm to 500 nm.
8 Notes: Step 2 was conducted with TFA/DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50.0%, Total Flow: 20 mL/min, Phase A: Hexane-HPLC, Phase B: IPA-HPLC, Column Name: Repaired DAICEL CHIRALPAK 1A, Length: 250 mm, Internal Diameter: 21.2 mm, Particle Size: 5 μιη, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1, Enantiomer A: lsl eluting peak (retention time ::: 11.8 min, 30 mg, 25%) as a yellow oil. LCMS (ESI, m/z): 561 [M+H]+. Step 1 , Enantiomer B: 2"d eluting peak (retention time = 15.6 min, 30 mg, 25%) as a yellow oil. LCMS (ESI, m/z): 561 [M+H]", Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 35.0%, End Cone, of Pump B: 52.0% Total Flow: 20 mL/min, Time: 7 nun, Phase A: Water (10 mmol/L NH4HCO3), Phase B: MeCN-HPLC, Column Name: XBridge Prep CT8 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μηι, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm.
9 Notes: Step 2 was conducted with TFA/DCM. Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient. Start Cone, of Pump B: 30%, Total Flow: 20 mL/mm, Phase A: Hexane (0.1% DEA), Phase B: EtOH-HPLC, Column Name: (R,R)-WHELK-01- romasil, Length: 25 cm, Internal Diameter: 5 cm, Particle Size: 5 um, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step 1 , Enantiomer A: lsl eluting peak (retention time = 13.3 min, 60 mg, 80%) as a yellow oil . LCMS (ESI, m/z): 590[M+H . Step 1, Enantiomer B: 2,id eluting peak (retention time = 15.6 min, 50 mg, 67%) as a yellow oil. LCMS (ESI, m/z): 590 [M+Hf. Prep HPLC Method for Step 2: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 18.0%, End Cone, of Pump B: 38.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water ( 10 mmol/L NH4HCO3), Phase B: MeCN-HPLC, Column Name: XBridge Prep CI 8 OBD Column, Length: 150 mm. Internal Diameter: 19 mm, Particle Size: 5 um, Aperture Size: 130 A, Column 'Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm
248
144628010 vl [M+H]
to 500 nm.
!0 Notes: HATU with DIEA in DMF was used for Step 1. Step 2 was conducted with TFA/DCM, Chiral HPLC conditions for Step 1: SHIMADZU LC-20AD, LC parameters: Pump Mode: Binar - gradient, Start Cone, of Pump B: 10.0%, Total Flow: l .OmL/min, Phase A:ACN(0.1%DEA), Phased: Methanol, Column Name: DAICEL CHIRALPAK AD-3, Length: 50 mm, Internal Diameter: 4.6 mm, Particle Size: 3 um, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. Step I, Enantiomer A: 1st eluting peak (retention time = 1.5 min, 50 mg, 77%) as a yellow solid. LCMS (ESI, m/z): 10 [M+H]+. Step 1, Enantiomer B: 2nd eluting peak (retention time = 2.3 min, 51mg, 78%) as a yellow solid. LCMS (ESI, m/z): 510 [M+H]+. isolation method: The title
compounds were isolated directly after Step 2 by trituration with diethyl ether and collection by vacuum filtration.
! '! Notes: Step 2 was conducted with TFA/DCM. Chiral HPLC coiidiiioMs for Step I Prep SFC350- 1, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50%, Total Flow: 150 mL/min, Phase A: CO?., Phase B: Methanol, Column Name: DAICEL CHIRALPAK AD-H, Length: 250 mm, Internal Diameter: 50 mm, Particle Size: 5 um. Column Temp: 20 °C, PDA Model: UV, Wavelength: 2,70 nm. Step 1, Enantiomer A: 1st eluting peak (retention time = 6,8 min, 300 mg, 65%) as a yello solid. LCMS (ESI, m/z): 546 [M+Hf. Step 1, Enantiomer B: 2nd eluting peak (retention time = 12.6 min, 300mg, 65%) as a yellow solid. LCMS (ESI, m/z): 546 [M+HJ+. HPLC Purification conditions: Waters, LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 18.0%, End Corse, of Pump B: 38.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (10 mmol/L
\1 1 ,1 ICO ,). Phase B: MeCN-HPLC, Column Name: XBndge Prep C18 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 um, Apeiture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A. Wavelength: from 190 nm to 500 nm.
Example 87 (1-113): 3-Amino-N-(2,5-difluoro-4- 'piperidin-4-yl)phenethyl)-6-methylthieno
[2,3-6]pyridine-2-carboxaniide
Figure imgf000250_0001
Example 87 (1-113)
144628010 vl Step 1. tert-Butyl 4-(4-(2-(3-amino-6-methylthieno [2,3-6 ] yridine-2-carboxamido)ethyl)- 2,5-difiuorophenyI)-3,6-dihydropyridine-l(2H)-carboxylate
[00525] Into a 100-mL round-bottom flask was added tert-butyl 4-[4-(2-aminoethyl)-2,5- difluorophenyl]-l,2,3,6-tetrahydropyridine-l -carboxylate (0.20 g, 0.59 mmol), 3-amino-6- methylthieno[2,3-6]pyridine-2-carboxylic acid (0.148 g, 0.710 mmol), dichloromethane (10 mL), HOBt (0.120 g, 0.78 mmol), DIEA (0.300 g, 2.3 mmol), and EDCI (0.171 g, 0.89 mmol). The resulting solution was stirred overnight at 40 °C and then cooled and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with dichloromethane/methanol (20: 1) to afford ferf-butyl 4-(4-(2-(3-amino-6-methylthieno[2,3- 6]pyridine-2-carboxamido)ethyl)-2,5-difluoro^ as yellow oil (70 mg, 22%). LCMS (ES, m/z): 529 [M+H]+.
Step 2. feflf-Butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)piperidine-l~carboxyIate
[00526] Into a 50-mL round-bottom flask that was purged and maintained under an aim op here of nitrogen was added fe/ -butyi 4-(4-(2-(3-amino-6-metb.ylthieno[2,3-6]pyridine-2- carboxamido)ethyl)-2,5-difluorophenyl)-3,6-dihydropyridine-l.(2H)-carboxylate (0.070 g, 0. 13 mmol), methanol (10 mL), 20% Pd(OH)2/'C (0.010 g, 0.21 mmol). The resulting mixture was sparged with hydrogen (balloon) and then stirred overnight at 40 °C under hydrogen (balloon). The solids were removed by filtration over Celite, and the filtrate was concentrated in vacuo to afford fer/-butyl 4-(4-(2~(3~amino~6~methylth^
difluorophenyl)piperidine-l -carboxylate as a brown solid (60 mg, 85%). LCMS (ESI, m/z): 531 M -H] .
Step 3, 3-Amino-N-(2,5-difluoro-4-(piperidin-4-yl)phenethyI)-6-methylthieno [2,3- 6jpyridine-2-carboxamide
[00527] Into a 50-mL round-bottom flask was added ferf-butyl 4-(4-(2-(3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)piperidine-l-carboxy
(0.060 g, 0.1 1 mmol) and dichloromethane (4 mL). Trifluoroacetic acid (0.7 mL) was added and the resulting solution was stirred for 3 h at RT. The reaction mixture was concentrated in vacuo to provide a caide product that was purified by Prep-HPLC using the following conditions: Column, X Bridge C18, 19* 150 mm, 5 um; mobile phase, A: water (containing 0.1% formic
250
144628010 vl acid) and B: CftCN (20% to 60% over 3 min; and then 60% to 80%; over 8 min); UV Detector: 254 nm. The purified product was then dissolved in small amount of MeOH and passed through a pre-packed cartridge (agilent, PL-HCC MP SPE 100 mg/6 mL) to remove formic acid to afford the title compound as a light yellow solid (10.4 mg, 21%). LCMS (ESI, m/z): 431 \\ - \ \ \ ¾-NMR (300 MHz, CD3OD) δ ppm 8,20 (d, J = 8,4 Hz, 11 1). 7,31 (d, ./ - 8.4 Hz, i i !), 7.01- 6,96 (m, 2H), 3.61-3,51 (m, 2H), 3.27-3.1 1 (m, 2H), 3.08 - 2.74 (m, 5H), 2.65 (s, 3H), 1.90-1.60 (m, 4H).
Example 88 (1-114): 3-Amino-6-cyclopropyl-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3- 6]pyridine-2-carboxamide
Figure imgf000252_0001
gjgp j Example 88 (1-114)
Step 1. 3-Amino-N-(4-bromophenethyl)-6-cyclopropylthieno[2,3-6]pyridine-2-carboxamide
[00528] Into an 8 mL reaction vial was added methyl 3-amino-6-cyclopropylthieno[2,3- £]pyridine-2-carboxylate (0.100 g, 0,403 mmol) and 2-(4-bromophenyl)ethanamine (0,121 g, 0,604 mmol) followed by a mixture of toluene/THF (10: 1; 2.2 mL), A solution of trimethy 3 aluminum (2 M in toluene, 0,604 mL, 1.21 mmol) was added and the reaction mixture was heated to 80 °C overnight. The reaction was quenched with a saturated solution of NaHCO, (2 mL), heated at 50 °C for 30 minutes, and then extracted with ethyl acetate (2 x 3 mL). The combined organic layers were washed with brine (3 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was suspended in ethyl acetate (2 mL) and sonicated for 15 minutes. The resulting white precipitate was filtered, washed with ethyl acetate (3 mL), and dried in vacuo overnight to afford 3-amino-Ar-(4-bromophenethyl)-6- cyclopropyithieno[2,3-/ ]pyridine-2-carboxamide (139 mg, 83 % yield), LCMS (ESI, m/z): 416
251
144628010 vl Step 2. ferf-lhitxi 4-(4-(2-(3-amino-6-cyclopropyIthieno[2,3-6]pyridine-2-carboxamido) ethyl)phenyI)piperazine-l-carboxylate
[00529] Into a 2 mL reaction vial was added 3-amino-Af-(4-bromophenethyl)-6- cyclopropylthieno[2,3-5]pyridine-2-carboxamide (0.013 g, 0.030 mmol), fert-butyl piperazine-1- carboxylate (0.011 g, 0.060 mmol), cesium carbonate (0.039 g, 0.12 mmol), XPhos Precatalyst 2nd Generation (0.006 g, 7.50 μηιοΐ), and dioxane (0.4 mL). The vial was flushed with nitrogen, sealed and heated overnight at 100 °C on a heater shaker. The reaction mixture was diluted with ethyl acetate (0.5 mL) and washed with saturated aqueous NaHCCb solution (0.5 mL). The organic layer was concentrated under a stream of nitrogen to afford crude fert-butyl 4-(4-(2-(3- amino-6-cyclopropylthieno[2,3-¾]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l- carboxylate. The material was used without further purification.
Step 3. 3-Amino-6-cyclopropyl-N-(4-(piperazin-l-yI)phenethyI)thieno 2,3-6]pyridine-2- carboxamide
[00530] Crude ierf-butyl 4-(4-(2-(3-amino-6-cyclopropylthieno[2,3- >]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate was taken up into MeOH/dioxane (1: 1; 300 μί.) and 4 N HC1 in dioxane (100 μΕ). The reaction was heated for 1 hour at 50 °C, concentrated to a cmde material that was dissolved in methanol (1 mL) and purified via SCX (500 mg) column chromatography. The impurities were eluted with methanol (3 mL) and the product was then eluted with a mixture of hexanes/EtOAc/Eti (10: 1 :0.5, 3 mL). The crude product was purified by mass triggered preparatory HPLC to afford the title compound (0.5 mg, 4 % yield). LCMS (ESI, m/z): 422 [M+H]+.
252
144628010 vl Example 89 (I- 115): 3,6-Diami8io-5-iluoro-A;-{4»(piperazin-i-yI)phenethy!)thieno[2,3- 6]pyridine-2-carboxamide
Figure imgf000254_0001
Example 89 (Ι-Π5)
Step 1. ferf-Butyl 4-(4-(2-(3-amino-6-((2,4-dimethoiybenzyl)amino)-5-fluorothieno[2,3- 6]pyridine-2-carboxamido)ethyi)phenyl)piperazine-l-carboxylate
[00531] Into a 100-mL 3-necked round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added ethyl 3-amino-6-[[(2,4-dimethoxyphenyl)methyl]amino]-5- fluorothieno[2,3-£]pyridine-2-carboxylate (0.300 g, 0,74 mmol), tert-but l 4-(4-(2- aminoethyl)phenyl)piperazine-l-carboxylate (0.271 g, 0.89 mmol), and toluene (20 mL). The reaction mixture was cooled to 0 °C and a solution of trimethylaluminum in heptane (1 M, 1.1 mL, 11.1 mmol) was added dropwise with stirring. The resulting solution was stirred overnight at 110 °C and then cooled and quenched by addition of MeOH (50 mL). The solids were removed by filtration and the filtrate was concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :2) to afford tert-butyl 4-(4-(2-(3- amino-6-((2,4-dimethoxybenzyl)amino)-5-fluoro^
phenyl)piperazine-l-carboxylate as a yellow oil (120 mg, 24%). LCMS (ESI, m/z): 665 j \i l f |
Step 2. 3,6-Diamino-5-fluoro-N-(4-(piperazin-l-y])phenethyi)thieno[2,3-6]pyridine-2- carboxamide
[00532] Into a 25-mL round-bottom flask was added tert-bulyl 4-(4-(2-(3-amino-6-((2,4- dimethoxybenzyl)amino)-5-fluorothieno[2,3-6]pyridine-2-carboxamido)ethyl)phenyl)piperazine- 1-carboxylate (0.100 g, 0.15 mmol) and dichloromethane (5 mL) followed by trifluoroacetic acid (3 mL) and the resulting solution was stirred for 2 h at RT. The reaction mixture was then
253
144628010 vl concentrated in vacuo to provide a crude product that was purified by Prep-HPLC using the following conditions (Waters I): Column, Xbridge Prep CIS OBD column, 5 μηι, 19* 150 mm; mobile phase, A: water (containing 10 raM NH3HCQ3 + 0.05% NH4OH) and B: CH CN (10% to 45 % over 5 min and then 45% to 75% over 5 min); UV Detector: 254 nm. This afforded the title compound as a white solid (28 nig, 45%). LCMS (ESI, m/z): 415 [M+H]+; ¾-NMR (300 MHz, DMS()-6¾) 6 ppra 7.96 (d, J = 11.8 Hz, 1 H), 7,43 - 7.33 (m, IH), 7.09 - 7.01 (m, 2H), 7.00 - 6.70 (m, 6H), 3.33 - 3.26 (m, 2H), 3.07 - 2.94 (m, 4H), 2.92 - 2.77 (m, 4H), 2.74 - 2.60 (m, 2H).
Example 90-1. ( -116): 3-Amino-N-(4-(5,6-dihydroimidazo[l,2-a]pyrazin-7(8jf7)- yI)phenethyI)-6-methylthieno[2,3-6]pyridine-2-carboxamide
Figure imgf000255_0001
Step J. 2-(4-(5,6-Dihydroimidazo[l,2-a]pyrazin-7(8H)-yl)phenethyl)isoindoline-l,3-dione
[00533] To a solution of 2-(4-bromophenethyl)isoindoline-l,3-dione (prepared according to the procedure described in Francis et al : Journal of Medicinal Chemistry (1991), 34(8), 2570- 2579; 0.2 M in anhydrous 1,4-dioxane; 150 μΕ, 30 μmol), was added 5,6,7,8- tetrah droimidazo[ 1 ,2- Jpyrazine (0,2 M in anhydrous 1 ,4-dioxane; 180 μΤ, 36 μηιοΐ), RuPhos solution (0.02 M in anhydrous 1,4-dioxane/EtOAc; 75 uL, 1.5 μmo!), RuPhos-Pd 2nd generation catalyst (0,02 M in anhydrous 1,4-dioxane, 75 μΐ., 1 ,5 μηιοΐ) and sodium fert-butoxide (2 M in THF; 150 μΐ^, 300 μΐΉθΙ). The resulting mixture was heated at 100 °C overnight. The reaction mixture was cooled to RT and used directly in Step 2 without further workup or i solation.
254
144628010 vl Step 2. 2-(4-(5,6-Dihydroimidazo[l,2-a]pyraziii-7(8H)-yl)phenyl)ethan-l-ainine
[00534] To the reaction mixture from Step 1 was added hydrazine solution (2 M in ethanol, 150 μΐ,, 300 μηιοΐ). The resulting mixture was heated to 80 °C overnight, brine (0.5 mL) was added, and the resulting mixture was extracted with ethyl acetate (2 x 0.5 mL). The combined organic layers were concentrated in vacuo to provide a crude product that was carried on without further purification.
Step 3. 3-AniiMO-Ar-(4-(5,6-di ydroimidazo[l,2-fl|pyrazin-7(8if -yI)phe5iet yl)-6- methylthieno[2,3-6]pyridine-2-carboxaniide
[00535] The crude product from Step 2 was dissolved in a solution of 10% DIEA in DMA (250 ^iL). 3-Amino-6-methylthieno[2,3-Z>]pyridine-2-carboxylic acid (0.2 M in DMA with 5%
DIEA; 150 μΤ,, 30 μηιοΐ) and ((I//-benzo[<i][l,2,3]triazo!-l-yi)oxy)tris(dimeihylamino) phosphonium hexafluorophosphate(V) solution (BOP reagent, 0.2 M in acetonitrile, 180 μΕ, 36 umol) were then added and the resulting mixture was placed on shaker at RT for 2 h. Brine (0.5 mL) was added and the mixture was extracted with ethyl acetate (2 x 0.5 mL). The combined organic layers were concentrated in vacuo to provide a crude product that was purified by HPLC: Waters Autopurification MS-directed HPLC prep fraction collection using the following conditions: Column: Waters XBridge OBD C I 8, 5 μπι, 19 x 50 mm; flow rate 20 ml/min; mobile phase, A: water with 0.1% ammonium hydroxide and B: methanol with 0.1% ammonium hydroxide using the following gradient: 0 to 2 mins ( 15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Detector in electrospray ionization mode. This afforded the title compound (5 mg, 37%). LCMS (ESI m/zy. 433 [M + H]
[00536] The Examples in Table 17 below were synthesized according to the procedures outlined above for Example 90-1 (1-116), using the appropriate synthetic precursors.
Table 17;
Figure imgf000256_0001
255
144628010 vl
Figure imgf000257_0001
Example 91-1 (1-1.21): (i5 -3-amino-6-methyl-N-(4-(3-methylpiperazin-l- yI)phenethyI)thieno[2,3-6]pyridine-2-carboxamide
Figure imgf000257_0002
Example 91-1 (1-121)
[00537] Steps 1-3. These steps were carried out as described above for Example 90-1 (1-116) using the appropriate synthetic precursors.
Step 4. (»S1)-3-amino-6-methyl-N-(4-(3-methylpiperazin-l-yl)phenethyi)thieno[2,3- 6]pyridine-2-carboxamide
[00538] The caide product obtained as described above from Step 3 was dissolved in methanol (0.4 mL) and 4 N HCl in dioxane solution (75 μ 300 μηιοΐ) was added. The resulting mixture was heated to 50 °C for 1 h and then concentrated in vacuo. The crude product
256
144628010 vl was purified by HPLC: Water Autopurification MS-directed HPLC prep fraction collection using the following conditions Column, Waters XBridge OBD C18, 5 μηι, 19 x 50 mm; flow rate 20 ml/min; mobile phase, A: water with 0.1% ammonium hydroxide and B: methanol with 0.1% ammonium hydroxide using the following gradient: 0 to 2 mins (15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Detector in e!ectrospray ionization mode. This afforded the title compound (6 nig, 46%). LCMS (ESI m/z): 410 [M+H]+.
[00539] The Examples in Table 18 below were synthesized according to the procedures outlined above for Example 91-1 (1-121), using the appropriate synthetic precursors.
Table 18:
Figure imgf000258_0001
257
144628010 vl
Figure imgf000259_0001
258
144628010 vl
Figure imgf000260_0001
259
144628010 vl
Figure imgf000261_0001
260
144628010 vl Example 92-1 (1-152): 3-Amino-N-(3-(l-(difluoromethyl)-li -pyrazol-4-yl)-4-(piperazin-i- yl)phenethyI)-6-methylthieno[2,3-i»]pyridine-2-carboxamide
Figure imgf000262_0001
Step 1. ferf-Butyl 4~(4-(2-aminoet yI)-2~bromop eeyI)psperazine~l~carboxyIate
[00540] Into a 250-mL round-bottom flask was added ferf-butyl 4-[4-(2- [[(benzyloxy)carbonyl]amino]ethyl)-2-bromophenyl]piperazine- 1-carboxylate (3.00 g, 5.79 mmol), methanol (80 ml .), and aqueous potassium hydroxide solution (40 %; 40 mL). The resulting solution was stirred for 8 h at 80 °C in an oil bath. The reaction was coo!ed to RT, and then was concentrated in vacuo to remove most of the organic solvent. The resulting mixture was taken up into water (50 mL), and extracted with dichlorom ethane (3 x 30 mL). The combined organic layers were washed with brine (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford fert-butyl 4-(4-(2-aminoethyl)-2- bromophenyl)piperazine-I-carboxylate (1.08 g) as a yellow oil. LCMS (ESI, m/z): 384, 386 | M 1 11 .
Step 2: fer/- But l 4-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- brosBopheiiyl)piperazine~l-carboxylate
[00541] To a solution of 3-amino-6-methylthieno[2,3-^]pyridine-2-carboxylic acid (0.416 g. 2 mmol) in DMF with 5% DIEA (10 mL) was added fert-butyl 4-(4-(2-aminoethyl)-2-
261
144628010 vl bromophenyi)piperazine-l-carboxylate (0.769 g, 2.00 mmol), followed by BOP ( 1.06 g, 2.4 mmol). The resulting mixture was stirred at RT for 2 h and then poured into a well-stirred ice- water mixture (25 niL). The resulting precipitate was collected by vacuum filtration, washed with water, and dried to afford fert-butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-Z>]pyridine-2- carboxamido)ethyl)-2-bromophenyl)piperazine-l-carboxylate (0.9 g, 78 % yield). The material was used without further purification.
Step 3. teri-Butyl 4-(4-(2-(3-amino-6-methyIthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- (l-(difluoromethyI)-l/ir-pyrazol-4-yl)phenyl)piperazine-l-carboxylate
[00542] To a solution of ferf-huiyl 4-(4-(2-(3-amino-6-methylthieno2,3-b][2,3-i»]pyridine-2- carboxamido)ethyl)-2-bromophenyl)piperazine-l-carboxylate in 1,4-dioxane (0.2 M in 1,4- dioxane; 150 L, 0,02 mmol) was added l-(difluoromethyl)-4-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)-lH-pyrazoie (0.2 M in 1,4-dioxane; 225 μΕ, 0.45 mmol) and potassium phosphate tribasic solution (1 M aqueous, 150 pL, 0.15 mmol). The mixture was sparged with nitrogen and then tetrakis(triphenylphosphine)palladium(0) (0.02 M in toluene, 75 μΙ_, 1.5 μηιοΐ) was added. The resulting mixture was placed in a shaker in a glove box under an atmophere of nitrogen atmosphere and heated at 80 °C overnight. After being cooled to RT, the mixture was diluted with brine (0.4 mL) and EtOAc (0.5 mL). The organic layer was separated and the aqueous layer was extracted again with EtOAc (0.6 mL). The combined organic layers were concentrated in vacuo and the material was used without further purification.
Step 4. 3-Amino-N-(3-(l-(difluoromethyl)-lH-pyrazol-4-yl)-4-(piperazin-l-yl)phenethyl)-6- methylthieno[2,3-6]pyridine-2-carboxamide
[00543] The crude product from Step 3 was re-dissolved in methanol (200 μΕ) and 4 N HCl/dioxane (75 μΐ., 0.3 mmol) was added. The mixture was placed on a shaker at 50 °C for 1 hour. The reaction mixture was concentrated in vacuo and the crude product was purified by HPLC using the following conditions: Waters Autopurification MS-directed FIPLC prep fraction collection using the following conditions Column, Waters XBridge OBD CI 8, 5 μιη, 19 x 50 mm; flow rate 20 ml/min; mobile phase, A: water with 0.1% ammonium hydroxide and B: methanol with 0.1% ammonium hydroxide running the following gradient 0 to 2 mins (15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Defector in electrospray ionization mode. This afforded the title compound (2 mg, 12%). LCMS (ESI, m/z): 512 [M+H]+.
262
144628010 vl [00544] The Examples in Table 19 below were synthesized according to the procedures outlined above for Example 92-1 (1-152), using the appropriate synthetic precursors.
Table 19:
Figure imgf000264_0001
263
144628010 vl
Figure imgf000265_0001
144628010 vl
Figure imgf000266_0001
Example 93 (1-168): 3-Am !¾o-6-roethy!~A^(4~(p8peraziii-l~yS)~3~(pyrid85i~2~y!)pheMethyl) thieno[2,3-6]pyridine-2-carboxamide
Figure imgf000266_0002
Example 93 (1-168)
Step 1. ferf-Butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-^]pyridine-2-carboxamido)ethyl)-2-
(pyridiii-2-yI)phe8iy!)p8perazme-l-carboxyIate
[00545] To a solution of fert-butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-Z>]pyridine-2- carboxamido)ethyi)-2 )romophenyl)piperazine-l-carboxylate in dioxane (0.2 M; 100 L, 20 pmol) was added a solution of pyridin-2-ylzinc (II) bromide (0.5 M Ί 1 II·. 200 pL, 100 μηιοΐ) and tetrakis(triphenylphosphine)palladium (0) (0.02 M toluene, 50 pL, 3 pmol). The resulting mixture was heated to 80 °C overnight. After being cooled to RT, brine (0.5 ml) was added and the resulting mixture was extracted with EtOAc (2 x 0.5 mL). The combined organic layers were concentrated in vacuo to afford crude tert-haiyl 4-(4-(2-(3-amino-6-methylthieno[2,3-^]pyri.dine- 2-carboxamido)ethyl)-2-(pyridin-2-yl)phenyl)piperazine-l-carboxylate. The material was used without further purification.
265
144628010 vl Step 2. 3-Ammo-0-methyI-A^4-(piperazm^
6]pyridine-2-carboxamide
[00546] The crude product from Step 1 was dissolved in methanol ( 400 μΐ.,), and 4 N HCl in dioxane (50 μΙ_, 200 μηιοΐ) was added. The resulting mixture was heated to 50 QC for 1 h and then cooled and concentrated in vacuo to provide a crude product that was purified by HPLC: Waters Autopurifi cation MS-directed HPLC prep fraction collection using the following conditions Column, Waters XB ridge OBD CIS, 5 μηι, 19 x 50 mm; flow rate 20 ml/min; mobile phase. A: water with 0.1% ammonium hydroxide and B: methanol with 0, 1% ammonium hydroxide (B) eluting with the following gradient; 0 to 2 mins (15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Detector in electrospray ionization mode. This afforded the title compound (3.7 mg, 39%). LCMS (ESI, m/z): 473 [ M I I I .
Example 94-1 (1-169): 3-Amino-N-(3-fluoro-4-(piperidin-4-yl)phenethyI)-6-methylthieno
Figure imgf000267_0001
Step 1. terf-Butyl 4-(4-(2-(3-amino-6-methyIthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- fluorophenyl)-3,6-dihydropyridine-l(2H)-carboxylate.
[00547] Into a 100-mL round-bottom flask was added tertAmiyX 4-[4-(2-aminoethyl)-2- fluorophenyi]-l,2,3,6-tetrahydropyridine-l-carboxylate (0,20 g, 0,62 mmol), dichloromethane (10 mL), 3-amino-6-methylmieno[2,3 ]pyridine-2-carboxylic acid (0, 192 g, 0.92 mmol), HOBt (0.127 g, 0,940 mmol), and EDO (0.18 g, 0,94 mmol). The resulting solution was stirred overnight at 40 °C. The solids were removed by filtration, and the filtrate was concentrated in vacuo to provide a crude produc that was purified via silica gel chromatography and eluted with dichloromethane/methanol (20: 1) to afford tert-butyl 4-(4-(2-(3-amino-6-methylthieno[2,3-
266
144628010 vl »]pyridine-2-carboxamido)ethyl)-2-fluorophenyi)-3,6-dihydropyridine-l(2H -carb as a light yellow oil (88 mg, 28%). LCMS (ES, m/z): 511 |'M~H ] .
Step 2. ferf-Butyl 4-(4-(2-(3-amino-6-methyIthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- fliiorophe y!)piperidiiie-l-carboxyIate
[00548] Into a 50-mL round-bottom flask purged and maintained with nitrogen was added tert-hwjl 4-(4-(2-(3-amino-6-methylthieno[2,3-/»]pyridine-2-carboxamido)ethyl)-2- fluorophenyl)-3,6-dihydropyridine-l(2H>carboxylate (0.088 g, 0.17 mmol), 20% Pd(OH)2/C (20 mg), and methanol (5 mL). The reaction mixture was sparged with \ \ , and the reaction mixture was stirred overnight at 40 °C. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford fer -butyl 4-(4-(2-(3- amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2-fluorophenyl)piperidine-l - carboxylate as a black oil (70 mg, 79%). LCMS (ESI, m/z): 513 | \i H j
Step 3. 3-Amino-N-(3-fluoro-4-(piperidin-4-yl)phenethyI)-6-methylthieno[2,3-6]pyridine-2- carboxamide 00549] Into a 50-mL round-bottom flask was added tert-buty\ 4-(4-(2-(3-amino-6- methylthieno[2,3-^]pyridine-2-carboxamido)ethyl)-2-fluorophenyl)piperidine-l-carboxylate
(0.070 g, 0.14 mmol) and dichloromethane (3 mL). Trifluoroacetic acid (0.6 mL) was added and the resulting solution was stirred for 2 h at RT, The reaction mixture was concentrated in vacuo and the crude product was diluted with methanol (5 mL). The pH of the solution was adjusted to approximately 8 with ammonium hydroxide and the caide product was then purified by Prep- HPLC using the following conditions: Column: SunFire Prep C 8 5 «&m 19* 150 mm; mobile phase, A: water (containing 0.05% ammonium hydroxide) and B: CEbCN (20% to 40 % over 8 min); UV Detector: 254 nm. This afforded the title compound as a light yellow oil (7.6 mg, 13%). LCMS (ESI, m/z): 413 [M+H] +; Ί [AMR (300MHz, CD3OD) δ ppm 8.21 (d, J = 8.1 Hz, 1H), 7.32 (d, J = 8.1 Hz, 1H), 7.23 (m, 1H), 7.07-6.98 (m, 2H), 3.55-3.50 (m, 2H), 3.30- 3.20 (m, 2H), 3.11-2.98 (m, 1H), 2.94-2.78 (m, 41 1 ). 2.65 (s, 3 ! 11, 1.91 -1.69 (m, 4H).
[00550] The Example in Table 20 below was synthesized according to the procedures outlined above for Example 94-1 (1-169), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appears below the example.
267
144628010 vl
Figure imgf000269_0001
Examples 95-A (1-171) md 95-B (1-172): Ar-(4-(8-azabicyclo[3.2,l]octan-3-yl)phenethyl
-2-
Figure imgf000269_0002
CH2
Step 3
HCI/Dioxane
Figure imgf000269_0003
A
Figure imgf000269_0004
Exampie 95-B (i-172): Diastereomer B
268
144628010 vl Step 1. ferf-Butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyI)-8-azabicycIo[3.2.1]oct- 2-ene-8-carboxylate
[00551] Into a 50-mL 3 -necked round-bottom flask, purged and maintained with nitrogen, was added benzyl N-[2-(4-bromophenyl)ethyl]carbamate (0.500 g, 1.50 mmol), fer -butyl 3-(4,4,5,5- tetramethyl-l,3,2-dioxaboro1an-2-yl)-8-azabicydo[3.2 ]oct-2-ene-8-carboxylate (0.553 g, 1.64 mmol), potassium carbonate (0.622 g, 4.50 mmol), Pd(dppi)Ch CH2Ci2 (0.243 g, 0.30 mmol), DMF (8 mL), and ethanol (2 mL). The reaction mixture was stirred for 2 h at 100 °C and then cooled to RT and diluted with water (10 mL). The resulting solution was extracted with ethyl acetate (3 x 30 mL) and the combined organic layers were washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resulting crude product was purified by FCC eluting with ethyl acetate/petroleum ether (1 :5) to afford tert-butyl 3-(4-(2- (((benzy3oxy)carbonyl)arnino) ethyl)phenyl)-8-azabicyclo[3.2. l]oct-2-ene-8-carboxylate as a yellow oil (340 mg, 49%). LCMS (ESI, nvz): 463 | M 1 11 .
Ste 2. tert-\l\\\ \ 3-(4-(2-aminoethyl)phenyl)-8-azabicyclo[3.2.1]octane-8-carboxylate
Figure imgf000270_0001
[00552] Into a 50-mL round-bottom flask, purged and maintained with nitrogen, was added fert-butyl 3-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenyl)-8-azabicyclo[3.2.1]oct-2-ene-8- carboxylate (0,33 g, 0.71 mmol), 1.0% palladium on carbon (100 mg), and methanol (10 mL). The reaction mixture was sparged with H2 and the reaction was stirred for 2 h at RT. The reaction was vented to nitrogen and the solids were removed by filtration over Celite. The filtrate was concentrated in vacuo to afford a cisi trans mixture of fer -butyl 3-(4-(2- aniinoethyl)phenyl)-8-azabicyclo[3.2.1]octane-8-carboxyiate as a yellow solid (220 mg, 94%). LCMS (ESI, m/z): 331 [M+H .
Step 3. ferf-Butyl 3-(4-(2-(3-amino-6-methylthieno[2,3-¾]pyridine-2-carboxamido)ethyi) phenyl)~8-azabicyclo[3.2.1]octane~8-carboxylate (cisltrans mixture)
[00553] Into a 50-mL round-bottom flask was added fert-butyl 3-(4-(2-aminoethyi)phenyi)-8- azabicyclo[3.2.1]octane-8-carboxylate (0.150 g, 0.45 mmol), dichloromethane (5 mL), 3-amino- 6-methylthieno[2, 3 -6]pyridme-2-carboxyli c acid (0.095 g, 0.46 mmol), EDCI (0.105 g, 0.55 mmol), HOBT (0.074 g, 0.55 mmol), and DIEA (0.176 g, 0.237 mL, 1.36 mmol). The resulting solution was stirred for 2 h at room temperature and then concentrated in vacuo to provide a
269
144628010 vl crude product that was purified by Prep-HPLC using the following conditions : Column, SunFire Prep C18 5 μηι 19*150um; mobile phase, Water (it contains 0,05¾TFA) and CftCN; Detector, 254 nm. This afforded a cisi trans mixture of fen-butyl 3-(4-(2-(3-amino-6-methylthieno[2,3- 6]pyridine-2-carboxamido)ethyl)phenyl)-8-azabicyclo[3.2. l]octane-8-carboxylate as a yellow solid (85 nig, 36%). ! CMS (ES, m/z): 521 i J ! ί ]
Step 4, N-(4-(8-azabicyclo[3.2.1]octan-3-yl)phenethyl)-3-amino-6-methylthieno[2,3- 6]pyridine-2-carboxainide (Diastereomers A and B)
[00554] Into a 25-mL round-bottom flask was added feri-butyl 3-(4-(2-(3-amino-6- methylthieno[2,3-0]pyridine-2-carboxamido)ethyl)phenyl)-8-azabicyclo[3.2.1]octane-8- carboxylate (0.030 g, 0.06 mmol) and methanol (1 mL) followed by 4 N Hydrogen chloride/dioxane (2 mL) and the resulting solution was stirred for 2 h at RT. The reaction mixture was concentrated in vacuo to provide a crude product that was purified by Chiral-Prep- HPLC using the following conditions: Column, DAICEL CHIRALPAK AD-3; mobile phase, A: ethano! (containing 0.1% DEA) and B: methanol; UV Detector: 254 nm. This afforded the title compounds as follows: 7.5 mg (31%) of N-(4-(8-azabicyclo[3.2.1]octan-3-yl)phenethyl)-3- amino-6-methylthieno[2,3-^]pyridine-2-carboxamide (Di aste eomer A, assumed cis, first eluting isomer, RT = 2.03) as an off-white solid and 4.5 mg (19%) of N-(4-(8-azabicyclo[3.2.1]octan-3- yi)phenethyl)-3-amino-6~meihylthieno[2,3-^]pyridine-2-carboxamide (Diastereomer B, assumed trans, second eluting isomer, RT = 4.41) as an off-white solid.
[00555] Example 95-A (1-171), Diastereomer A: LCMS (ESI, m/z): 421 [M+H]+; ! i-YMR (300 MHz, Acetone-^) δ ppm 8.24 (d, 8.0 Hz, 1H), 7.30 (d, ./ 8,2 Hz, 1H), 7.20 (s, 4H),
3.64 - 3.48 (m, 4H), 2.94 - 2.86 (m, 2H), 2.61 (s, 3H), 2.10 (s, 2H), 1.84 - 1.76 (m, 4H), 1.75 - 1.72 (m, IH), 1.71 - 1.64 (m, 21 s )
[00556] Example 95-B (1-172), Diastereomer B: LCMS (ESI, m/z): 421 [M+H]+; ! i MR (300 MHz, CD3OD) δ ppm 8.21 (d, J = 8.3 Hz, IH), 7.36 - 7.20 (m, 5H), 3.68 (br s, 2H), 3.57 - 3.49 (m, 2H), 3.04 - 2.98 (m, IH), 2.91-2,86 (m, 2H), 2.66 (s, 3H), 2.38 - 2,25 (m, 2H), 1.96 -
1.65 (m, 6H).
270
144628010 vl Example 96-1 (1-1.73): 4-(2-(3-Amino-6-methylthieno[2,3-djpyridine-2-carboxamido)ethyl) benzoic acid
Figure imgf000272_0001
Example 96-1 (1-173)
Step 1, Methyl 4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)benzoate
[00557] Into a 100-mL round-bottom flask was added 3-amino-6-methylthieno[2,3-b][2,3- yridine~2-carboxyiic acid (0.581 g, 2.79 mmol), DMF (8 mL), HATU (1.59 g, 6.61 mmol), methyl 4-(2-aminoethyl)benzoate (0.500 g, 2.79 mmol), and DIE A (1.08 g, 8.36 mmol). The reaction mixture was stirred for 2 h at room temperature and then extracted with ethyl acetate (3 x 20 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and e!uted with ethyl acetate/petroleum ether (1 :1 ) to afford methyl 4-(2-(3-amino-6- methylthieno[2,3-5]pyridine-2-carboxamido)ethyl)benzoate as a yellow solid (520 mg, 50%). LCMS (ESI, m/z). 370 [M+H]+.
Step 2. 4-(2-(3-Amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)benzoic acid
[00558] Into a 50-mL round-bottom flask was added methyl 4-(2-(3-amino-6- methylthieno[2,3-0]pyridine-2-carboxamido)ethyl)benzoate (0,500 g, 1.35 mmol), sodium hydroxide (0.271 g, 6.78 mmol) in ), THF (3 mL) and water (2 mL). The resulting solution was stirred for 4 h at 60 °C. The pH of the solution was adjusted to approximately 2 with aqueous HC1 (1 M) and the resulting precipitate was collected by filtration and dried in vacuo. The crude product was purified by Prep-HPLC using the following conditions (waters 2767): Column, XBridge RP1.8 19 x 150 mm, 5 μηι; mobile phase, A : Water (containing 10 mM NH4HCO3 + 0.05% ammonium hydroxide) and B: MeCN (8% to 15% over 8 rain.); Flow rate: 20 mL/min, UV Detector: 254 nm. This afforded the title compound as a light yellow solid (1 1.9 mg, 2%). LCMS (ESI, m/z): 356 [M+ff ; 1H NMR (300 MHz, DMSCW s δ ppm 8.29 (d, ./ 8.4 Hz, 1 H),
271
144628010 vl 7.84 (d, J = 8.1 Hz, 2H), 7.77-7.73 (m, 1H), 7.32-7.28 (m, 3H), 7.12 (br s, 2H), 3.46 - 3.39 (m, 2H1 2.97-2.86 (m, 2H), 2.57 (s, 3H).
[00559] The Examples in Table 21 below were synthesized according to the procedures outlined above for Example 96-1 (1-173), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appears below each example.
Table 21:
Figure imgf000273_0001
272
144628010 vl
Figure imgf000274_0001
273
144628010 vl Example 97 (1-179): 3-Amino-N-(3-c Ioro-4-(piperazin-l-yl)phenethyl)-6-(difluoromethyl) thieno[2,3- >]pyridine-2-carboxaniide
Figure imgf000275_0001
Step J, tert- utyl 4-(2-chloro-4-(2-(2-chioroaceiamido)ethyl)phenyl)piperazine-l- carboxylate
[00560] Into a 100-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added tert-hutyl 4-[4-(2-aminoethyl)-2-chlorophenyl]piperazine-l-carboxylate (0.456 g, 1 ,34 mmoi), tetrahydrofuran (6 mL), and DIEA (0,348 g, 0.469 ml. 2.69 mmol) followed by the dropwise addition of 2-chloroacetyl chloride (182 mg, 1.61 mmol). The resulting solution was stirred for 3 h at RT. The reaction mixture was concentrated in vacuo to provide a crude product that was purified by FCC eluting with petroleum ether / ethyl acetate (1 : 1) to afford tert-butyl 4-(2-chloro-4-(2-(2-chloroacetamido)ethyl)phenyl)piperazine-l- carboxylate as a yellow oil (200 mg, 36%). LCMS (ESI, mfz) 416 [M+H]÷.
Step 2. tert-ΒιύγΙ 4-(4-(2-(3-amino-6-(dimethoxyniethyI)thieno[2,3-6jpyridine-2- carboxamido)ethyI)-2-chlorophenyl)piperazine-l-carboxylate
[00561] Into a 50-mL round-bottom flask that purged and maintained under an inert atmosphere of nitrogen was added tert-butyl 4-(2-chloro-4-(2-(2-chloroacetamido)ethyl) phenyl)piperazine-l-carboxylate (0.200 g, 0.48 mmol), 6-(dimethoxymethyl)-2-sulfanylpyridine- 3-carbonitrile (0.123 g, 0.59 mmol), NaOMe (0.130 g, 2.41 mmol), and tetrahydrofuran (2 mL). The reaction mixture was stirred overnight at 50 °C and then cooled and concentrated in vacuo to
274
144628010 vl provide a crude product that was purified by FCC eluting with petroleum ether / ethyl acetate (1 :3) to afford ferf-butyl 4-(4-(2-(3-amino-6-(dimethoxymethyl)thieno[2,3-^]pyridine-2- carboxamido)eihyl)-2-chlorophenyl)piperazine-l-carboxylate as a yellow oil (315 mg). The material was used without further purification. LCMS (ESI, m/z) 590 [M+H]"' .
Step 3, ferf-Butyl 4-(4-(2-(3-amino-6-formyIthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- cSiIoropheiiyl)piperazine-l-carboxyiaie
[00562] Into a 50-mL round-bottom flask was added fe/ -butyl 4-(4-(2-(3-amino-6- (dimethoxymethyl)thieno[2,3-6]pyridine-2-carboxamido)ethyl)-2-chlorophenyl)piperazine-l- carboxylate (0.40 g, 0.68 mmol) and aqueous AcOH (80%; 6 mL). The resulting solution was stiixed for 2 h at 70 °C and then cooled to RT and concentrated in vacuo to provide a crude product that was dissolved in DCM (5 mL). The pH of the solution was adjusted to approximately 7 with saturated aqueous sodium bicarbonate. The solids were removed by filtration and the filtrate was extracted with DCM (20 mL) and washed with brine (2 x 10 ml). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford ten-butyl 4-(4-(2-(3-amino-6-formylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2- chlorophenyl) piperazine-l-carboxylate as a yellow solid (30 mg, 8%). LCMS (ESI, m/z) 544 [ ! !j .
Step 4, ferf-Butyl 4-(4-(2-(3-amino-6-(difluoromethyl)thieno[2,3-6]pyridine-2- carboxamido)ethyl)-2-chlorophenyl)piperazine-l-carboxylate
[00563] Into a 50-mL round-bottom flask, purged and maintained under an inert atmosphere of nitrogen, was added feri-butyl 4-(4-(2-(3-amino-6-formylthieno[2,3-A]pyridine-2- carboxamido)ethyl)-2-chlorophenyl)piperazine-l-carboxylate (0.080 g, 0.15 mmol) and dichloromethane (4 mL). The resulting mixture was cooled to -70 °C and then a solution of BAST (0.325 g, 1.47 mmol) in dichloromethane (1 mL) was added dropwise. The resulting solution was warmed and stilted overnight at RT. The reaction mixture was then cooled to -70 °C, quenched with methanol (2 mL), and concentrated in vacuo to provide a cmde product that was purified by FCC eluting with petroleum ether/ethyl acetate (1 : 1) to afford fenf-butyl 4-(4~(2~ (3-amino-6-(difluoromethyl)thieno[2,3-^]pyridine-2-carboxamido)ethyl)-2-chlorophenyl) piperazine-l-carboxylate as a yellow oil (40 mg, 48%). LCMS (ESI, m/z) 566 [M+H]+.
275
144628010 vl Step 5. 3-Amino-N-(3-chloro-4-(piperazin-l-yl)phenethyl)-6-(difluoromethyi)thieno[2,3- 6]pyridine-2-carboxamide
[00564] Into a 50-mL round-bottom flask was added lert-huty\ 4-(4-(2-(3-amino-6- (difluoromethyl)thieno[2,3- i]pyridine-2-carboxamido)ethyl)-2-chlorophenyl)piperazine-l- carboxylate (0,020 g, 0.04 mmol) and 4 N hydrogen chloride/dioxane (3 mL). The solution was stirred for 2 h at RT and then concentrated in vacuo to provide a crude product that was purified by Prep-HPLC using the following conditions: Column, X Bridge C I 8, 19* 150 mm, 5 μηι; mobile phase. A: water (containing l OmM NH4HCO3 + 0.05% ammonium hydroxide) and B: MeCN (15% to 45% over 4 min and then 45% to 80% over 8 min); Flow rate: 15 ml/min; UV Detector: 254 nm. This afforded the title compound as a yellow solid (3.0 mg, 18%). LCMS (ESI, m/z) 466 [M+H]+; !l l NMR (300 MHz, CD3OD) δ ppm 8.47 (d, J = 8.7 Hz, 1H), 7.70 (d, J = 8.1 Hz, IH), 7.36-7.32 (m, 1H), 7.24-7.11 (m, 1H), 7.10-7.06 (m, 1H), 6.82 (t, J = 55.2 Hz, 1H), 3.57-3.52 (m, 2H), 3.25 - 3.20 (m, 4H), 3.17 - 3 ,15 (m, 4H), 2.90-2.85 (m, 2H).
Examples 98-A (1-180) and 98-B (1-181): (/?)-3-amino-6-methyl-N-(4-(pyrrolidin-3- yI)phenethyl)thieno[2,3-6]pyridine-2-carboxainide (stereochemical configuration assumed) and (^)-3-amino-6-methyI-N-(4-(pyrrolidin-3-yI)phenethyl)thieno[2,3-6]pyridine-2- carboxamide (stereochemical configuration assumed)
Figure imgf000277_0001
Example 98-A (1-180), Enantiomer A Example 98-B (1-181), Enantioiner B
Step 1. Racemic tert-bvtyl 3-(4-(2-(3-amino-6-methyIthienoi2,3-6]pyridine-2-carboxamido) ethyl)phenyI)-3-hydroxypyrrolidine-l-carboxylate
[00565] Into a 100-mL round-bottom flask was added fen-butyl 3-[4-(2-aminoethyl)phenyI]- 3-hydroxypyrrolidine-l-carboxylate (3.00 g, 9.79 mmol), 3-amino-6-methylthieno[2,3- £]pyridine-2-carboxylic acid (1.63 g, 7.83 mmol), HATU (3.73 g, 9.81 mmol), DIEA (3.79 g,
276
144628010 vl 5.11 mL, 29.33 mrnoi), and DMF (30 mL). The resulting solution was stirred for 4 h at RT and then water (100-mL) was added. The resulting solution was extracted with ethyl acetate (3 x 50- mL) and the combined organic layers were concentrated in vacuo to provide a crude product that was purified via silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 1) to afford raceniic tert-bu yl 3-(4-(2-(3-amino-6-methylthieno[2,3-i»]pyridine-2-carboxamido)ethyl) phenyl)-3 -hydroxypyrrolidine- 1 -carboxylate as a yellow solid (280 mg, 6%). LCMS (ESI, m/z): 497[M-4-lf.
[00566] Step 2. Enantiomer A: (i?)-3-amino-6-methyl-N-(4-(pyrrolidin-3- yl)phenethyl)thieno[2,3-6]pyridine-2-carboxamide (stereochemical configuration assumed), and Enantiomer B: (.S)-3-amino-6-methyl-N-(4-(pyrrolidin-3-yl)phenethyl)thieno[2,3- 6]pyridine-2-carboxamide (stereochemical configuration assumed)
[00567] Into a 50-mL round-bottom flask was added racemic fen-butyl 3-(4-(2-(3-amino-6- methylthieno[2,3-¾]pyridine-2-carboxamido)ethyl)phenyl)-3-hydroxypyrrolidirie-l-carboxyiate (0.200 g, 0.40 mmol), EfeSiH (0.935 g, 8.06 mmol), and dichloromethane (20 mL). The resulting solution was stirred for I h at RT. TFA (0.459 g, 0.308 mL, 4.03 mrnoi) was added and the resulting solution was stirred for 4 h at RT. The reaction mixture was then concentrated in vacuo to provide a crude product that was purified by Prep-HPLC using the following conditions (waters 2767): Column, X Bridge C18, 19* 150 mm, 5 μιη; mobi!e phase, A: water (containing 10 fflM H4HCO3 + 0.05% ammonium hydroxide) and B: CH3CN (15% to 30% over 3 min, and the 30% to 75% over 8 min), Flow rate: 20 mL/min; UV Detector: 254 nra. 80 mg of pure product was obtained as a mixture of stereoisomers. The mixture was then purified by ChiraS- Prep-HPLC using the following conditions (SHIMADZU LC-20AD): Column, DAICEL CHIRALPAK AD-3; mobile phase, Phase A: Acetonitrile (0.1% DEA) Phase B: Ethanol; UV Detector: 190 nm to 500 nm. This afforded the title compounds as follows:
[00568] Enantiomer A: 8.8 mg (6%) as a yellow solid (retention time = 2.38 min). LCMS (ESI, m z): 381 [M+H]÷; lH NMR (300 MHz, CD3OD) d ppm 8.19 (d, ./ - 8.4 Hz, 1 H), 7.32 (d, - 8.1 Hz, 5H), 7.26-7.22 (m, 4H), 3.62- 3.39 (m, 5H), 3,26 - 3.11 (m, I I I). 3,01 - 2,83 (m, 3H), 2.65 (s, 31 1 ). 2.41- 2.28 (m. i l l ). 2.05 -1.89 (m, 1H).
[00569] Enantiomer B: 2.8 mg (2%) as a yellow oil (retention time = 3,04 min), LCMS (ESI, m/z): 381 \ \\ \ \ \ ¾ NMR (300 MHz, CD3OD) 5 ppm 8.20 (d, J = 8.1 Hz, i l l). 7.42 -
277
144628010 vl 7.12 (m, 5H), 3.62- 3.32 (m, 5H), 3.26■■■■ 3.1 1 (m, 1H), 3.01- 2.83 (m, 3H), 2.65 (s, 3H), 2.41- 2.28 (m, 1H), 2.08 -1.92 (m, 1H).
Example 99 (1-182): 3-Amino-N-(3-hydroxy-4-(piperazin-l-yl)phenethyl)-6-niethylthieno
[2,3-6]pyridine-2-carboxamide
Figure imgf000279_0001
Example 99 (1-182)
Step 1. terf- butyl 4-(4-(2-(3-amino-6-methylthieno(2,3-6]pyridine-2-carboxamido)ethyl)-2- hydroxyphenyl)piperazine-l-carboxylate
[00570] To a solution of tert-hutyl 4-(4-(2-(3-amino-6-methylthieno[2,3-^]pyridine-2- carboxamido)ethyl)-2-bromophenyl)piperazine-l-carboxylate (0.2 M in 1,4-dioxane, 150 iiL. 0,03 rnmo!) was added aqueous cesium hydroxide (2 M; 300 μΐ.,, 0.6 mmol), di-fert-butyl(2',4',6'- triisopropyl-3,6-dimethoxy-[l,r-biphenyl]-2-yl)phosphine (tBuBrettPhos, 0.02 M in 1,4- dioxane, 240 μΐ,, 4,80 μίηοΐ) and tris(dibenzylideneacetone)dipalladium(0) (0.02 M in 1,4- dioxane, 30 iL, 0.6 μηιοΐ). The resulting mixture was heated to 80 °C overnight. After being cooled to RT, brine (0.5 mL) was added and the mixture was extracted with ethyl acetate (2 x 0.5 mL). The combined organic layers were concentrated in vacuo to afford crude tert-butyl 4-(4-(2- (3-amino-6-methylthieno[2,3-Z>]pyridine-2-carboxamido)ethyl)-2-hydroxyphenyl)piperazine-l- carboxy!ate which was used in the next step without further purification.
Step 2. 3-Araino-N-(3-hydroxy-4-(piperazin-l-yl)phenethyl)-6-methylthieno[2,3-6]pyridine-
2-carboxaniide
[00571] The crude product from Step 1 was dissolved in methanol (0.4 mL) and then 4 N HC1 in dioxane (75 μΐ,, 0.3 mmol) was added. The resulting mixture was heated to 50 °C for 1 h and then concentrated in vacuo to provide a crude product that was purified by HPLC (Waters Autopurification MS-directed HPLC prep fraction collection using the following conditions:
278
144628010 vl Column: Waters XBridge OBD C18, 5 μηι, 19 x 50 mm; flow rate 20 mL/min; mobile phase, A: water with 0.1% ammonium hydroxide and B: methanol with 0.1% ammonium hydroxide running the following gradient 0 to 2 mins (15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Detector in electrospray ionization mode) to afford 3-amino-N"-(3-hydroxy-4-(piperazin-l- yi)phenethyl)-6--methylthieno[2,3-5]pyridine-2-carboxamide (1.8 mg, 15%). LCMS (ESI m/z): 412 [M - H] .
Example 100-1. (1-183): 3,6-Diamino-4-methyl-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3-
£>]pyridiiie~2~carboxamide
Figure imgf000280_0001
Step 1. ferf-Butyl 4-(4-(2-(3-amino-6-((i,eif-butoxycarbonyl)amino)-4-methylthieno[2,3- 6]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate
[00572] To a suspension, of fert-butyl 4-(4-(2-(3-amino-6-chloro-4-methylthieno[2,3- 6]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (100.0 mg, 0.189 mmol), tert- butyl carbamate (1 10 mg, 0.943 mmol), and cesium carbonate (307 mg, 0.943 mmol) in 1,4- dioxane (1 ml) was added XPhos Pd 2nd generation precatalyst (37.1 mg, 0.047 mmol). Nitrogen was bubbled through the reaction mixture for 2 minutes and then the reaction was heated to 90 °C overnight. The reaction was cooled to R.T and the crude product was purified by FCC eluting with 20 - 40% EtOAc in hexanes. The resulting product was dissolved in 1 : 1 MeOH/water (2 mL) and purified by RP HPLC (C I 8 column, gradient: 0 ~ 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes). Lyophi!ization afforded the title compound as a light yellow solid (24 mg, 20.8%). LCMS (ESI, m/z): 611 | \H l j '
279
144628010 vl Step 2. 3,6-Diamino-4-methyi-N-(4-(piperaziii-l-y])phenethyi)thieno[2,3-6]pyridine-2- carboxamide
[00573] Trifluoroacetic acid (100 μΐ, 1.535 mraol) was slowly added to a solution of ler l-buty\ 4-(4-(2-(3-amino-6-((ter^butoxycarbonyl)amino)-4-methylthieno[2,3- >]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (20.0 mg, 0.033 mmol) in dichloromethane (1.0 mi ). The reaction was stirred at RT for 2 hours and then concentrated in vacuo to provide a crude product that was dissolved in MeOH (1 mL) and purified by RP HPLC (CI 8 column; gradient; 0 - 90% MeCN in water containing 0, 1% ammonium hydroxide over 15 minutes). Lyophilization afforded the title compound as a white powder (3.1 mg, 49%). LCMS (ESI, m/z)A \ 1 I - f i j ; Ί ί NMR (300 MHz, DMSO- / ) δ ppm 7,33 (m, I I I). 7,05 (d, J = 8,5 Hz, 21 1), 6.84 (d, ./ 8.8 Hz, 2H), 6.64 (s, 2H), 6.44 (s, 2H), 6. 19 (s, 1 1 1 ), 3.34 (m, 2H), 2.98 (m, 4H), 2.82 (m, 4H), 2.70 (m, 21 1 ). 2,54 (s, 3H).
[00574] The Examples in Table 22 below were synthesized according to the procedures outlined above for Example 100-1 (1-183), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as FIPLC purification conditions appears the table below.
Table 22:
Figure imgf000281_0001
280
144628010 vl
Figure imgf000282_0001
containing 0.1% formic acid over 8 minutes)
Example 101-1 (1-186): 3-Amino-6-(dimethylamino)-N-(4-(piperazin-l-yl)phenethyl)thieno 2,3~6]pyridiiie~2-carboxamide
Figure imgf000282_0002
Step 1. ferf-Butyl 4-(4-(2-(3-amin0~6-(dimeihySamisio)ihieno[2,3-6]pyridioe-2-carboxaiiiido) ethyl)phenyl)piperazine-l-carboxylate
[00575] To a solution of fert-butyl 4-(4-(2-(3-amino-6-chlorothieno[2,3-Z>]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0,030 g, 0.058 mmol), and dimethyl amine (2.0 M in THF, 1.0 mL, 2.0 mmol) was added DIEA (0.030 mL, 0.174 mmol). The reaction mixture was heated in a sealed tube at 100 °C overnight and then cooled and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 50 - 70% EtOAc in hexanes to afford tert-butyl 4-(4-(2-(3-amino-6-(dimethyiamino)thieno[2,3-Z>]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate as a light yellow solid (18.9 mg, 62%). I .CM (ESI, m/'z) 525 [M+H]+.
281
144628010 vl Step 2, 3-Amino-6-(dimethylamino)-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3-6|pyrid
2-earboxamide
[00576] Trifluoroacetic acid (1.00 mL, 15.3 mmol) was slowly added to a solution of fen- butyl 4-(4-(2-(3-amino-6-(dimethylamino)thieno[2,3-i]pyridine-2-carboxamido)ethyl)phenyl) piperazine-l-carboxylate (0.018 g, 0.034 mmol) in dichlorom ethane (2.0 ml). The reaction was stirred at RT for 2 hours and then concentrated in vacuo to provide a crude product that was then dissolved in DMSO (1 mL) and purified by RP HPLC (CI 8 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes). Lyophilization afforded the title compound as a white powder (13 mg, 87%). ! CMS (ESI, m/z): 425 [M+H]+; I I NMR (300 MHz, DMSO-t/ ) δ ppm 8.05 (d, ./ 9, 1 Hz, 1H), 7,30 (m, I H), 7.06 (d, J = 8.5 Hz, 2H), 6,99 (s, 2H), 6.84 (d, J = 8.5 Hz, 21 1 ). 6.74 (d, J = 9.1 Hz, IH), 3.30 (m, 2H), 3.10 (s, 6H), 2.99 (m, 4H), 2.82 (m, 4H), 2.68 (m, 2H).
[00577] The Examples in Table 23 below were synthesized according to the procedures outlined above for Example 101-1 (1-186), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as FIPLC purification conditions appears in the table below.
Table 23:
Figure imgf000283_0001
282
144628010 vl
Figure imgf000284_0001
Example 102- L90): 3-Amino-6-methoxy-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3- 6]pyridine-2
Figure imgf000284_0002
[00578] To a solution of fe/ -buty! 4-(4-(2-(3-amino-6-chlorothieno[2,3- >]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.050 g, 0.097 mmol) in MeOH (1 .0 mL) was added sodium methoxide (25 wt% in MeOH, 0.250 g, 1.157 mmol). The reaction mixture was heated to 1 00 °C in a sealed tube overnight and then cooled to RT and concentrated in vacuo to afford the crude product. The cmde product was dissolved in DMSO (1 mL) and was purified by RP HPLC (CI 8 column, gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide ov 1 5 minutes). Lyophilization afforded the title compound as a white powder (15 mg, 37%). LCMS (ESI, m/z): 412 [M+H]+; lH NMR (300 MHz, DMSO-6¾) δ ppm 8.30 (d, ./ 8,8 Hz, 1H), 7.59 (m, 1H), 7.1 1 (s, 2H), 7.06 (d, J = 8.8 Hz, 2H), 6.85 (ra, 3H), 3.93, (s, 3H), 3.34 (m, 2H), 2.98 (m, 4H), 2.81 (m, 4H), 2.70 (m, 2H). [00579] The Examples in Table 24 below were synthesized according to the procedures outlined above for Example 102-1 (1-190), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appears below each example.
Table 24;
Figure imgf000285_0001
284
144628010 vl Example 103 (1-193): 3-Amino-6-(difluoromethoxy)-A7-(4-(piperazin-l-yl)p enethyl)
Figure imgf000286_0001
Step 3 Example 103 (1-193)
Stei) J. tert- (2-(3-amino-6-oxo-6,7-dihydrothieno[2,3-6]pyridine-2-carboxamido)
1-carboxvlate
)] To a solution of /er/-butyl 4-(4-(2-(3-amino-6-chlorothieno[2,3-£]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.050 g, 0.097 mmol) in DMSO ( 1.0 ml) and water (1.0 ml) was added sodium hydroxide (0.039 g, 0.969 mmol). The reaction mixture was heated to 100 °C overnight and then cooled to RT, diluted with brine ( 10 mL), and extracted with EtOAc (2 x 25 mL). The combined organic layers were washed with brine (10 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 60 - 80% EtOAc in hexanes to afford tot-butyl 4-(4-(2-(3-amino- 6-oxo-6,7-dihydrothieno[2,3-Z)]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l -carboxylate as a light red solid (19 mg, 39%). LCMS (ESI, m/z): 498 [M-H] .
Step 2. fert-Butyl 4-(4-(2-(3-amino-6-(difluoromethoxy)thieno[2,3-i»]pyridine-2- carboxamido)ethyI)phenyl)piperazine-l-carboxylate
[00581] Sodium hydroxide (0.003 g, 0.066 mmol) was added to a solution of fer -butyl 4-(4- (2-(3-amino-6-oxo-6,7-dihydrothieno[2,3-A]pyridine-2-carboxamido)ethyl)phenyl)piperazin^ carboxylate (30 mg, 0.060 mmol) and sodium 2-chloro-2,2-difluoroacetate (18.38 mg, 0.121 mmol) in DMF (0.5 ml). The reaction mixture was heated to 55 °C overnight, and then cooled and quenched with water (5 mL). The reaction was extracted with EtOAc (2 x 10 mL). The combined organic layers were washed with saturated sodium bicarbonate (aq, 10 mL) and brine (10 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 50 - 70%, EtOAc in hexanes to afford tert-buXsA 4-
285
144628010 vl (4-(2-(3-amino-6-(difluoromemoxy)thieno[2,3-Z>]py
piperazine-l-carboxylate as a white solid (18 mg, 54%). LCMS (ESI, m/z): 548 [M+H]+.
Step 3. 3-Amino-6-(difluororaethoxy)-N-(4-(piperazin-l-yI)phenethyl)thieno[2,3-6] pyridine-2-carboxamide
[00582] TFA (0.5 mL, 7.67 mmol) was slowly added to a solution of fer -butyi 4-(4-(2-(3- amino-6-(difluoromethoxy)thieno[2,3-Z>]pyridin^
carboxylate (15.0 mg, 0.027 mmol) in dichlorom ethane (1.0 mi). The reaction was stirred at RT for 2 hours and then concentrated in vacuo to provide a crude product that was dissolved in DMSO (1 mL) and was purified by RP HPLC (C18 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes). Lyophilization afforded the title compound as a white powder (8.7 mg, 71 %), LCMS (ESI, m/z): 448 [M+H]+; lE NMR (300 MHz, DMSG-ifc) δ ppm 8.52 (d, J = 8.8 Hz, 1H), 7.80 (s, IH), 7.76 (m, IH), 7.20 (s, 2H), 7.13 (d, J --- 8.8 Hz, I H), 7.05 (d, J = 8.5 Hz, 2H), 6.84 (d, J = 8,5 Hz, 2H), 3,34 (m, 2H), 2,98 (m, 4H), 2.81 (m, 4H), 2.73 (m, 2H).
Example 104-1 (1-194): 3-Amino-6-(methyIainino)-N-(4-(l-methyIpiperidin-4- yI)phenethyl)thienol2,3-6]pyridine-2-carboxamide
Figure imgf000287_0001
[00583] To a solution of 3-amino-6-chloro-N-(4-(l -methylpiperidin-4- yl)phenethyl)thieno[2,3- »]pyridine-2-carboxamide (0.100 g, 0.233 mmol) and methanamine (2.0M in Ti ll . 2.0 mL, 4.00 mmol) was added DIEA (0.122 mL, 0.699 mmol). The reaction mixture was heated to 100 °C overmght in a sealed tube and then cooled and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 60 - 80%o EtOAc in hexanes to afford 3-amino-6-(methylamino)-.V-(4-(l-me
]pyridine~2-carboxamide as a light yellow solid (10.4 mg, 1 1%). LCMS (ESI, m/z): 510 i Xi - l i f: ¾ NMR (300 MHz, i)\ I S< )-./<·) δ ppm 7,90 (d, ./ = 8,8 Hz, IH), 7,32 (m, I H), 7. 15 (m, 5H), 6.94 (s, 2H), 6.48 (d, ./ 8.8 Hz, IH), 3.33 (m, 3H), 2.83 (m, 51 1 L 2.73 (m, 2H), 2.18 (s, 3H), 1.95 (m, 2H), 1 .69 (m, 4 H).
286
144628010 vl [00584] The Examples in Table 25 below were synthesized according to the procedures outlined above for Example 104-1 (1-194), using the appropriate synthetic precursors. Additional detail around the synthetic methods as well as HPLC purification conditions appears below each example.
Table 25;
Figure imgf000288_0001
287
144628010 vl Example 105 (1-197): 3-Amino-5-cyclopropyl-6-methyl-N-(4-(piperazin- yl)phenethyI)thienof2,3-6]pyridine-2-carboxamide (hydrochloride salt)
Figure imgf000289_0001
xamp e -
Step 1. tertSutyl 4~(4-(2-(3~ammo-5~bromo-6~methy!ihie o 2,3~fe]pyridiise~2~carboxamido) ethyl)phenyI)piperazine-l-carboxylate
[00585] To a solution of 3-amino-5-bromo-6-methylthieno[2,3-i]pyridine-2-carboxylic acid (0,400 g, 1.39 mmol), ferf-butyl 4-(4-(2-aminoethyl)phenyl)piperazine-l-carboxylate (0.425 g, 1.393 mmol), DMAP (0.017 g, 0.139 mmol), and HBTU (0.634 g, 1.672 mmol) in DMF (10.0 ml) was added DIEA (0.728 ml, 4.18 mmol). The reaction was stirred at 80 °C overnight and then cooled to RT. Water (10 mL) was added, and a light orange precipitate formed. The precipitate was collected via filtration, washed sequentially with saturated aqueous ammonium chloride (10 mL), aqueous sodium bicarbonate (10 mL), water (10 mL), and ether (10 mL). The precipitate was collected, suspended, and slightly sonicated in EtOAc (10 mL). The suspended solid was then collected by filtration and dried in vacuo to afford fert-butyl 4-(4-(2-(3-amino-5- bromo-6-methylthieno[2,3-Z»]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate as a light grey powder that was carried on without further purification (88%). LCMS (ES, m/z): 575 ! X I ί i J .
Step 2. ferf-Butyl 4-(4-(2-(3-ainino-5-cyclopropyl-6-methylthieno[2,3-6]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate
[00586] To a suspension of ter -butyl 4-(4-(2-(3-amino-5-bromo-6-methylthieno[2,3- &]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.050 g, 0.087 mmol), cyclopropylboronic acid (0,007 g, 0.087 mmol), and cesium carbonate (0, 142 g, 0.435 mmol) in 1,4-dioxane (1 ml) was added XPhos Pd 2nd generation precatalyst (0.017 g, 0.022 mmol). The
288
144628010 vl reaction mixture was sparged with nitrogen for 2 minutes and then the reaction was heated to 90 °C overnight. The reaction mixture was cooled and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 40 - 60% EtOAc in hexanes to afford /er /-butyl 4- (4-(2-(3-amino-5-cyclopropyl-6-methylthien^
piperazine-l-carboxylate as a light yellow solid (32 mg, 68%), LCMS (ESI, m/z): 536 [M+H]÷
Step 3. 3-Amino-5-cyclopropyl-6-methyl-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3- 6]pyridine-2-carboxamide (hydrochloride salt)
[00587] To a solution of /er/-butyl 4-(4-(2-(3-amino-5-cyclopropyl-6-methylthieno[2,3- 6]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.030 g, 0.056 mmo!) in dioxane (1.0 ml) was added 4 N HCi in dioxane (0.50-mL, 2.0 mmol) at RT. The reaction solution was stirred for 2 hours resulting in a precipitate. The reaction mixture was diluted with ether (2 mL) and the precipitate was collected by vacuum filtration. The precipitate was further washed with ether (1 mL), collected, and dried / / vacuo to afford the title compound as a light orange solid (9.1 nig, 34%). LCMS (ESI, m/z): 436 [M+Hf; ¾ NMR (300 MHz, DMSO-ifc) 5 ppm 8.05 (s, I I I ), 7.68 (m, lH), 7.10 (s, 2H), 7.04 (d; J = 8.5 Hz, 21 1 ), 6,86 (d. ./ 8.5 Ήζ, 21 1), 3.35 (m, 2H), 2.91 (m, 4H), 2.73 (m, 4H), 2.67 (m, 5H), 2.04 (m, 1H), 1.01 (m, 2H), 0.98 (ni,
Example 106 (1-198): 3,5-Diamino-6-methyl-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3- 6]pyridine-2-carboxamide
Figure imgf000290_0001
289
144628010 vl Step 1. ferf-Butyl 4-(4-(2-(3-aiiiiiio-5-((teri-butoxycarbonyl)amino)-6-inethylthieno [2,3- 6]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate 0058S] To a suspension of tert-butyl 4-(4-(2-(3-araino-5-bromo-6-methylthieno[2,3- Z»]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (0.050 g, 0.087 mmol), tert- butyl carbamate (0,051 g, 0.44 mmol), and cesium carbonate (0, 142 g, 0,435 mmol) in 1,4- dioxane (1 ml) was added XPhos Pd 2 generation precataiyst (0.017 g, 0.022 mmol). The reaction mixture was sparged with nitrogen for 2 minutes and then heated at 90 °C overnight. The reaction mixture was cooled and concentrated in vacuo to provide a crude product that was purified by FCC eluting with 40 - 60% EtOAc in hexanes to afford fe/ -butyl 4-(4-(2-(3-amino- 5-((fert-butoxycarbonyl)amino)-6-niethylthieno[2,3-6]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate as a light yellow solid (39 mg, 78%). LCMS (ES, m/z): 611 j .
Step 2. 3,5-Diamino-6-methyl-N-(4-(piperazin-l-yI)phenethyl)thieno[2,3-6jpyridine-2- carboxamide
[00589] To a solution of tert-butyl 4-(4-(2-(3-amino-5-((te^butoxycarbonyl)amino)-6- methy lthieno[2,3 -£]pyri dine-2-carboxamido)ethy 1 )phenyl)pi perazine- 1 -carboxylate (0.035 g, 0.057 mmol) in DCM (2.0 ml) was added trifluoroacetic acid (0.50-mL, 7,67 mmol) at RT. The reaction solution was stirred for 2 hours and then concentrated in vacuo to provide a crude product that was dissolved in 1 : 1 DMSO/water (2 mL) and purified by RP HPLC (CI 8 column; gradient: 0 - 90% MeCN in water containing 0.1% ammonium hydroxide over 15 minutes), Lyophilization afforded the title compound as a white powder (15 mg, 65%). LCMS (ESI, m/z): 411 [M-H] . Ή NMR. (300 MHz, D SCW- ) δ ppm 7.52 (m, 1H), 7.38 (s, 1H), 7.05 (d, ./ 8.5 Hz, 2H), 6.90 (s, 21 1 ). 6.84 (d, J = 8.5 Hz, 2H), 5.16 (s, 2H), 3.29 (m, 2H), 2.98 (m, 4H), 2.82 (m, 4H), 2,69 (m, 2H), 2.39 (s, 3H),
290
144628010 vl Example 107 (1-199): 3-Amino-N-(2,5-difluoro-4-(3-(met yIamino)azetidin-l-yl)phenethyl)- 6-methylthieno [2,3-6] py r idine-2-carboxamide
Figure imgf000292_0001
Step 1. fert-Butyl (l-(4-(2-(3-amino-6-methylthieno[2^-6]pyridine-2-carboxamido)ethyl)- 2,5-difluorophenyI)azetidin-3-yl)(methyl)carbamate
[00590] Into a 25-mL round-bottom flask was added 3-ammo-6-methylthieno[2,3-6]pyridine- 2-carboxy!ic acid (0.1 1 g, 0.53 mmol), tert~ uiy\ iV-[l-[4-(2-aminoethyl)-2,5- difluorophenyl]azetidin-3-yl]-Ar-methylcarbamate (0.18 g, 0.53 mmol), HOBt (0.086 g, 0.63 mmol), EDCI (0.120 g, 0.63 mmol), DIEA (0.205 g, 1.58 mmol), and dichloromethane (10 niL). The resulting solution was stirred for 2 h at 40 °C and then cooled and concentrated in vacuo to provide a crude product that was purified by Prep-HPLC using the following conditions (Waters I); LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B; 15.0%, End Cone, of Pump B: 70.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (0.1% FA), Phase B: MeCN-HPLC, Column Name; XB ridge Prep CI 8 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μηι, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded ferf-butyl (l-(4-(2-(3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl) azetidin-3- yl)(methyl)carbamate as a yellow solid (60 mg, 21%). LCMS (ESI, m/z): 532 [M+H]+.
Step 2. 3-Amino-N-(2,5-difluoro-4-(3-(methylamino)azetidin-l-yl)phenethyl)-6- methylthieno[2,3-6]pyridine-2-carboxamide
[00591] Into a 50-mL round-bottom flask was added tert-hutyl (l -(4-(2-(3-amino-6- methylthieno[2,3-0]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)azetidin-3-ylXmethyl) carbamate (0.060 g, 0.11 mmol), A'-methyl morpholine (0,034 g, 0.037 mL, 0,34 mmol), and
291
144628010 vl dichlororaethane (10 mL) followed by the dropwise addition of trimethylsilyi iodide (0.045 g, 0.032 mL, 0,23 mmol) with stirring at 0 °C. The resulting solution was stirred for 1 h at 0 °C and then concentrated in vacuo to provide a crude product that was purified by Prep-HPLC using the following conditions (SHTMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 25,0%, End Cone, of Pump B: 38.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (10 mmol/L Μ ί ιί ΚΌ ). Phase B: MeCN-HPLC, Column Name: XBridge Prep CI 8 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μηι, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 390 nm to 500 nm. This afforded 3-Amino-/V-(2,5-difluoro-4-(3-(methylamino)azetidin-l- yl)phenethyl)-6-methylthieno[2,3-Z»]pyridine-2-carboxamide as a white solid (15 mg, 30%). LCMS (ESI, mizy, 432 [M+Hf. ¾ NMR (400 MHz, CD3OD) δ ppm: δ 8.18 (d, J = 8.0 Hz, 1H), 7.29 (d, J = 8,4 Hz, 1 1 1 ), 6.87 - 6.92 (m, i l l ). 6.24 -· 6,29 (m, 1H), 4.13 - 4.15 (m, 2H), 3,57 -- 3.64 (m, 3H), 3.46 -■ 3.49 (m, 2H), 2.78 -■ 2.81 (m, 2H), 2.63 (s, 3H), 2.33 (s, 3H).
Example 108-1 (1-200). (i?)-3-ainino-N-(4-(3-(ethylamino)pyrroIidin-l-yl)phenethyi)-6- no ■2-
Figure imgf000293_0001
dioxane. 100 °C
Step 1 4 N HCI/dioxane
eOH, 50 °C
Step 2
Figure imgf000293_0002
/. tert~Buiy\ (/?)-(l-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2- carboxaRiido)ethyl)p enyl)pyrrolidiE-3-yI)carbamate
[00592] To a solution of 3-amino-N-(4-bromophenethyl)-6-methylthieno[2,3-0]pyridine-2- carboxamide (0.55 g, 1.4 ramol) in anhydrous 1,4-dioxane (10 mL) was added
Figure imgf000293_0003
pyrrolidin-3-yl carbamate (0.39 g, 2.1 mmol), XPhos Pd-G2 precatalyst (0.055 g, 0.07 ramol) and cesium carbonate (1 ,8 g, 5.6 mmol). The resulting mixture was heated under an atmosphere of
292
144628010 vl nitrogen at 100 °C overnight. After being cooled to RT, the reaction mixture was partitioned between brine and ethyl acetate, The layers were separated and the aqueous layer was extracted with EtOAc again. The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The caide product that was purified by Prep-TLC (50% EtOAc in hexanes) to afford ferr-butyl (/ -(l -(4-(2-(3-amino-6-metlvylthieno[2,3-/)]pyridine-2- carboxamido)ethyl)phenyl)pyrroiidin-3-yl)carbamate (98 mg, 14%).
Step 2. {R)-3-affliii0-iY~{4-(3-aniiii0pyrroI^^
pyridine-l-carboxamsde
[00593] To a solution of tot-butyl (i?)-(l-(4-(2-(3-amino-6-methylthieno[2,3-^]pyridine-2- carboxaraido)ethyi)phenyi)pyriOlidin-3-yl)carbamate (0.098 g, 0.20 mmol) in methanol (2 mL) was added 4 N HCl/dioxane solution (0.5 mL, 2 mmol). The resulting mixture was heated to 50 °C for 1 h, cooled to RT, and concentrated in vacuo. The crude product was used without further purification. LCMS (ESL m/z): 396 | M I f j .
Step 3. (/?)-3-amino-N-(4-(3-(ethyIamino)pyrrol^
pyridine-2-carboxamide
[00594] To a solution of crude (R)-3-amino-N"-(4-(3-aminopyrrolidin-l -yl)phenethyl)-6- methylthieno [2,3-6]pyridine-2-carboxamide from Step 2 above (0.2 M in 5% DIEA/DCM, 100 μΕ, 0.02 mmol) was added acetaldehyde (0.2 M in 10% HOAc/DCM, 120 μΕ, 0.024 mmol). The mixture was placed on a shaker for 30 min and then a sodium triacetoxyborohydride solution (0.2 M in DCM, 200 tuL, 0.04 mmol) was added. The mixture was placed on shaker at RT overnight. The reaction mixture was partitioned between 0.5 mL of 1 N NaOH in brine and 0.7 mL of ethyl acetate. The organic layer was separated and the aqueous layer was extracted again with EtOAc (0.7 mL). The combined organic layers were concentrated in vacuo to provide a crude product that was purified by HPLC using the following method: Waters Autopurification MS-directed HPLC prep fraction collection using the following conditions Column, Waters XBridge OBD C I 8, 5 μηι, 19 x 50 mm; flow rate 20 mL/min; mobile phase, A: water with 0.1% ammonium hydroxide and B: methanol with 0.1% ammonium hydroxide running the following gradient 0 to 2 mins (15% B), 2 to 6 mins (15-100% B); Detector ZQ Mass Detector in electrospray ionization mode. This afforded the title compound (5 mg, 54%). LCMS (ESI, m/z): 424 | M i l | .
293
144628010 vl [00595] The Example in Table 26 below was synthesized according to the ures outlined above for Example 108-1 (1-200), using the appropriate synthetic precursors,
Table 26:
Figure imgf000295_0003
Example 109-1 (1-209). (i?)-3-ainino-N-(2,5-difluoro-4-(3-methylpiperazin-l-yl)phenethyl)-
6-methv l-Ci
Figure imgf000295_0001
1 ) t-BuXPhos Pd G4
Cs2C03
Figure imgf000295_0002
Example 109-1 (I-209)
Step 1. 3-amino-N-(4-bromo-2,5-difluorophenethyl)-6-methylthieno [2,3-6] pyridine-2-
into a 50-mL round-bottom flask, was placed a solution of 2-(4-bromo-2,5- difluorophenyl)ethan-l -amine (930 rng, 3.94 mmol) in dichloromethane (100 mL), 3-amino-6- methylthieno[2,3-b]pyridine-2-carboxyliG acid (820 mg, 3.94 mmo!), EDCI (908 rag, 4.74 mmol), HOBT (638 mg, 4.72 mmol), and DIEA (1.53 g, 11.8 mmol). The resulting solution was stirred overnight at RT. The reaction was then quenched by the addition of 50 mL of water. The resulting solution was extracted with 3 x 50 mL of dichloromethane and the organic layers combined. The resulting mixture was washed with brine. The mixture was dried over anhydrous
»4
144628010 vl sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :1) to afford 3- amino-N-(4-bromo-2,5-difiuorophenethyi)^^ (780 nig, 46%) as a light yellow solid. LCMS (ESI, m/z): 428 [ \f · ! ! ] ' .
Step 2, 3~ammo~N-(2,5-diflisoro~4-iodop!
carfooxasmde
[00597] Into reaction vial were added sodium iodide (0.030 g, 0.2 mmol), copper(I) iodide (1.9 mg, 0.01 mmol), 3-amino-N-(4-bromo-2,5-difluorophenethyl)-6-methylthieno[2,3- b]pyridine-2-carboxamide (0.043 mg, 0.1 mmol), 700 uL n-BuOH and 0.2 M solution of N1,N2- dimethyl ethane- 1 ,2-di amine (0.200 mL, 0.04 mmol) in n-BuOH. The reaction mixture was degassed with nitrogen, sealed and heated at 120 °C overnight. The reaction was allowed to cool down and worked-up with 10% aqueous ammonia and ethyl acetate. The organic layer was washed with brine, dried over anhydrous Na2S04, filtered, and concentrated in vacuo to afford 3-amino-A-(2,5-difluoro-4-iodophenethyi)-6-meihylthieno[2,3-9/]pyridine-2-carboxamide (38 mg, 69 %), LCMS (ESI, m/z): 474 [M+H]+.
Step 3. ( ?)-3-amino-N-(2,5-difluoro-4-(3-methylpiperazin-l-yl)phenethyl)-6- methylthieno[2,3-6]pyridine-2-carboxamide 0059S] The reaction vial was charged with 3-amino-N-(2,5-difluoro-4-iodophenethyl)-6- methylthieno[2,3-#]pyridine-2-carboxamide (15 mg, 0.032 mmol), (R)-tert-but l 2- methylpiperazine-l-carboxylate (15.87 mg, 0.079 mmol), cesium carbonate (51.6 mg, 0.158 mmol), t-BuXPhos pre-catalyst G4 (5.12 mg, 6.34 μπιοΐ) and 300 μΙ_, DMF Dioxane (2: 1) mixture, The reaction vial was flushed with nitrogen, sealed and stirred at 92 °C for 4 hours, The reaction mixture was allowed to cool down, diluted with 1 mL Ethyl Acetate and filtered through SiliaPrepMB DMT 200 mg SPE cartridge to remove palladium catalyst. The cartridge was washed with 2 mL EA and volatiles were evaporated. The residue was dissolved in 1 mL methanol and loaded on 500 mg SCX SPE cartridge. The impurities were eluted with 3 mL methanol and the product was eluted with 3 mL 2M Hs in methanol. Volatiles were evaporated and the resulting crude material was used directly without further purification. The material was dissolved in 100 μΕ MeOH and 100 μΤ, Dioxane and 4N HC1 (79 μΐ, 0,317 mmol) in Dioxane was added. The reaction was heated at 50 °C for 30 min, cooled to RT and then concentrated.
295
144628010 vl The residue was azeotroped with toluene, dissolved in 500 μΐ, DMSO and purified by prep HPLC to afford (/?)-3-amino-A-(2,5-difliioro-4-i3-nieth}dpiperazin-l -yl)phenethyl)-6- methylthieno[2,3~^]pyridine-2-carboxamide (0,5 rng, 3.54 %). I CMS (ESI, m/z): 446 [M+H .
[00599] The Example in Table 27 below was synthesized according to the procedures outlined above for Example 109-1 (1-209), using the appropriate synthetic precursors.
Table 27:
Figure imgf000297_0001
296
144628010 vl Examples 110-A (1-211) and 110-B (1-212). 3-Ainino- V-(2,5-difluoro-4-((3Sr,4i;)-3-fluoro-4- amino)pyriOlidin-l-yl)phenethyl)-6-methylthieno[2,3-6]pyridine-2-carboxainide
3-Amino-N-(2,5-difluoro-4-((3 f,4S)-3-fiuoro-4-(methylamino)pyrroIidin-l- iyl)-6-methylthieno [2,3-6] pyridine-2-carboxamide
Figure imgf000298_0001
Enantiomer A Enantiomer B
BBr3, CH2Clj, -30 "C BBr3, CH2Ci2, -30 °C
1 Step 7
Figure imgf000298_0002
Example 110-A (E-211) Example 110-B {!-212}
Step 1. ds-fert-Butyl-3-[[(benzyloxy)carbonyl]amino]-4-fluoropyrrolidine-l-carboxylate
[00600] Into a 100-mL round-bottom flask, was placed cis ftrr-butyl-3-aniino-4- fluoropyrrolidine-l -carboxylate (1 ,0 g, 4,9 mmol), potassium carbonate (2.0 g, 14.7 mmol), benzyl chloroformate (835 mg, 4.9 mmol), ethyl acetate (15 mL) and water (15 mL). The resulting solution was stirred for 4 h at 60 °C in an oil bath. The resulting solution was cooled to room temperature and extracted with 3 x 10 mL of ethyl acetate, washed with 30 mL of brine,
144628010 vl dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum to afford cis-tert- butyl-3-[[(benzyloxy)carbonyl]amino]-4-fluoropyrrolidine-l-carboxylate as a yellow oil (1.5 g crude). LCMS (ESI, ra/z); 339 [ M i l | .
Step 2. cis tert-Butyl 3-(((benzyloxy)carbonyl)(methyl)amino)-4-fluoropyrrolidine-l- carboxylate
[00601] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed c/5-t<??"i-butyl-3-[[(benzyloxy)carbonyl]amino]-4-fluoropyrrolidine-l - carboxylate (1.5 g, 4.43 mrnoi) and THF (20 mL). This was followed by the addition of sodium hydride (2.66 g, 6.65 mmol, 60% dispersion in oil) at 0 °C. The mixture was stirred for 20 min at 0 °C. To this was added CKbl (944 rag, 6.65 mmol). The resulting solution was stirred for 14 h at room temperature. The resulting mixture was diluted with 20 mL of water, extracted with 3 x 15 mL of ethyl acetate, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 : 10) to afford cis te t-butyl 3- (((benzyloxy)carbonyl)(methyl)amino)-4-fluoropyrrolidine-l -carboxylate as a light yellow oil (1.2 g, 77%). LCMS (ESI, m/z): 353 [M+H]+.
Step 3, cis Benzyl (4-fluoropyrrolidin-3-yl)(methyl)carbamate
[00602] Into a 50-mL round-bottom flask, was placed cis tert-butyl 3- (((benzyloxy)carbonyl)(methyl)amino)-4-fIuoropyrrolidine-l-carboxylate (600 nig, 1.70 mmol), dichloromethane (15 mL) and TFA (3 mL). The resulting solution was stirred for 2 h at room temperature. The pH value of the solution was adjusted to 8 with ammonia. The resulting mixture was exiracted with 3 x 100 mL of ethyl acetate, washed with 100 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum to afford cis benzyl (4- fluoropyrrolidin-3-yl)(methyl)carbamate as a brown solid (420 mg crude). LCMS (ESI, m/z): 253 [ M ! i | .
Step 4, cis Benzyl (l-(4-(2-((tert-butoxycarbonyl)amino)ethyl)-2,5-difluorophenyI)-4- fluoropyrroIidin-3-yI)(methyl)carbamate
[00603] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed cis benzyl (4-fluoropyrrolidin-3-yl)(methyl)carbamate (360.0 mg, 1.43
298
144628010 vl mmol), tert-butyl (4-bromo-2,5-difluorophenethyl)carbamate (479.7 mg, 1 .43 mmol), Pd(OAc)2 (32.0 mg, 0.14 mmol), XPhos (68.0 mg, 0.14 mmol), CS2CO3 (228 mg, 0,70 mmol ) and toluene (10 mL). The resulting solution was stirred for 6 h at 80 °C in an oil bath, and then cooled to room temperature and diluted with 20 mL of water. The resulting mixture was extracted with 3 x 20 mL of ethyl acetate, washed with 50 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 : 10) to afford cis benzyl (l-(4- (2-((tert-butoxycarbonyl)amino)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yi)(methyl)carbamate as a yellow solid (580 mg, 80%). LCMS (ESI, rn/z) 508 [M+H]+.
Step 5 cis Benzyl (l-(4-(2-aminoethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yl)(methyl)carbamate
[00604] Into a IGO-mL round-bottom flask was placed cis benzyl (l-(4-(2-((tert- butoxycarbonyl)amino)ethyl)-2,5-difluorophenyl )-4-t]uoropyrrolidin
(560 mg, 1.10 mmol) and dichloromethane (25 mL). This was followed by the addition of trifluoroacetic acid (5 mL) at 0 °C. The resulting solution was stirred for 2 h at room temperature. The pH value of the solution was adjusted to 8 with ammonia. The resulting mixture was extracted with 3 x 20 ml, of dichloromethane, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum to afford cis benzyl (l-(4-(2-aminoethyl)-2,5- difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyl)carbamate as a brown solid (380 mg, crude), LCMS (ESI, mil): 408 | \ ! H i
Step 6. Benzyl ((3J?,4tST)-l-(4-(2-(3-a!Mino-6-methyIt ieno[2,3-6]pyridine-2- carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyl)carbamate and Benzyl ((3»S,4R,)-l-(4-(2-(3-aniino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)-4-fluoropyrrolidin-3-yI)(methyl)carbamate
[00605] Into a 25-mL round-bottom flask was placed cis benzyl ( 1 -(4-(2-aminoethyl)-2, 5- difluorophenyl)-4-f!uoropynOlidin-3-yl)(methyl)cai"bamate (210.0 mg, 0.52 mmol), 3-amino-6- methylthieno[2,3-Z»]pyridine-2-carboxylic acid (107.6 mg, 0.52 mmol), EDO (129.8 mg, 0.68 mmol), HOBT (83,8 mg, 0.62 mmol), D1EA (133.6 mg, 1.04 mmol) and DMF (5 mL). The resulting solution was stirred for 14 h at 40 °C. The resulting mixture was diluted with 10 mL of water and extracted with 3 x 10 mL of ethyl acetate. The organic layers were combined, dried
299
144628010 vl over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 : 1). The racemate (270 mg) was separated by Chiral-Prep-HPLC using the following conditions (SH ADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 30.0%, Total Flow: 20 mL/min, Phase A Hexanes, Phase B: Ethanol, Column Name: CHIRALCEL OJ-H, Length: 25 mm, Internal Diameter: 2 mm, Particle Size: 5 μηι, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This resulted in the following: Step 6, Enasitioraer A: 1st eiuting peak (retention time = 11.6 min, 50 mg, 12% yield, yellow solid) assigned as benzyl ((3R,4S)-l-(4-(2-(3-amino-6-methylthieno[2,3- ¾]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yl)(methyl)carbamate. LCMS (ESI, m/'z): 598 [M+H]+; and Step 6, Eiiantiomer B: 2nd eiuting peak (retention time ::: 13.1 min, 45 mg, 1 1% yield, yellow solid) assigned as benzyl ((3S,4R)-1- (4-(2-(3-amino-6-methylthieno[2,3- >]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)-4- fluoropyrrolidin-3-y])(methyl)carbamate. LCMS (ESI, m/z): 598 [M+H] .
Step 7. 3-Amino-N-(2,5-difluoro-4-((35,4i?)-3-fluoro-4-(inethylaiiiino)pyrrolidin-l- yI)phenethyI)-6-inethylthieno[2,3-6]pyridine-2-carboxamide
[00606] Into a 25 -mL round-bottom flask was placed benzyl ((3R,4S)-l-(4-(2-(3-amino-6- methylthieno[2,3- >]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yl)(methyl)carbamate (Step 6, Enantiomer A) (40 mg, 0.07 mmol) and dichloromethane (2 mL). Then a solution of BBn in dichloromethane (1 M) (2, 1 mL, 2.10 mmol) was added at -30 °C, The resulting solution was stirred for Ih at -30 °C in a liquid nitrogen bath, and then concentrated under vacuum. The pH value of the solution was adjusted to 8 with ammonia. The resulting mixture was extracted with 3 x 10 mL of ethyl acetate. The organic layers combined, washed with 10 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by Prep-HPLC using the following conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 20.0%, End Cone, of Pump B: 22.0%, Total Flow: 20 mL/min, Time: 17 min, Phase A: Water (0.05% TFA), Phase B: MeCN-HPLC, Column Name: XBridge BEH CI 8 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 pm, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded 3-amino-JV-(2,5-
300
144628010 vl difluoro-4-((3S,4R)-3-fluoro-4-(methylamino)py
Z>]pyridine-2-Garboxamide as a light, yellow solid (2,4 mg, 8%). LCMS (ESI, m/∑) 464 [M+H]+, Ί i-NMR. (300 MHz, Methanol -d4) δ ppm 8.15 (d, ./ 8, 1 Hz, 1H), 7,26 (d, ./ 8.4 Hz, 11 1 ),, 6.79 - 6.96 (m, 1H), 6.26 - 6.48 (m, H I). 5.1 1-5.31 (m, 1H), 3.71-3.90 (m, 1H), 3.46 - 3.67 (m, 4H), 3, 16 -■ 3.44 (ra, 2! I ). 2.77 (t, J= 7.2 Hz, 2H), 2,60 (s, 3! ! }, 2,46 (s, 3H).
Step 7. 3-Amino-N-(2,5-difluoro-4-((3R,4S)-3-fluoro-4-(methylamino)pyrrolidin-l- yI)phenethyI)-6-methylthieno[2,3-6]pyridine-2-carboxamide
[00607] The same synthetic procedure described to prepare 3-amino-A'-(2,5-diiluoro-4- ((3S,4R)-3-fluoro-4-(methylamino)pyrrolid ^
carboxamide was applied to Step 6, Enantiomer B except using the following Prep-HPLC conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 31.0%, End Cone, of Pump B: 53.0% Total Flow: 20 mL/min, Time: 7 min, Phase A: Water (10 mmol NH4HCO3), Phase B: MeCN-HPLC, Column Name: XBridge BEH C18 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μτη, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded 3-amino-N-(2,5-difluoro-4-((3R,4S)-3-fluora
yl)phenethyl)-6-methylthieno[2,3-0]pyridine-2-carboxamide as a yellow solid (2.3 mg, 10%). LCMS (ESI, m/z): 464 | M ! ! ! , ¾- MR (300 MHz,
Figure imgf000302_0001
δ ppm 8.15 (d, J = 8,4 Hz, 1H), 7.26 id. ./ 8.4 Hz, 1H), 6.79 - 6.96 (m, 1H), 6.26 - 6.48 (m, 1H), 5.11 - 5.30 (m, 1H), 3.72 - 3.92 (m,lH), 3.44 - 3.66 (m, 4H), 3 , 16 - 3 ,39 (m, 2H), 2.77 (t, J = 7.2 Hz, 2H), 2.60 (s, M i l 2.46 (s, M l ).
301
144628010 vl Examples 111-A (1-213) and 111-B (1-214). 3-Araino-N-(2,5-difluoro-4-((3i?,4i?)-3-fiuoro-4- (methylamino)pyriOlidin-l-yl)phenethyl)-6-methylthieno[2,3-6]pyridine-2-carboxamide and 3-Amino-N-(2,5-difluoro-4-((31S,4S)-3-fiuoro-4-(methylamino)pyrroHdin-l- yI)phenethyI)-6-methylthieno[2,3-6]pyridine-2-carboxainide
Figure imgf000303_0001
Figure imgf000303_0002
111-B -214)
Step 1. tram Benzyl 3-azido-4-hydroxypyrrolidine-l-carboxylate
[00608] Into a 500-mL round-bottom flask, was placed benzyl 6-oxa-3- azabicyclo[3 1.0]hexane-3-carboxylate (10,0 g, 45.6 mmol), acetone (80 mL), water (20 mL) and Na 3 (6,0 g, 92,3 mmol). The resulting solution was stirred for 18 h at 80 °C. The reaction mixture was cooled to room temperature, and then quenched by the addition of sat. aq. sodium carbonate solution (50 mL). The resulting mixture was extracted with 3 x 200 mL ethyl acetate, washed with 200 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (2:3) to afford trans benzyl 3-azido-4-hydroxypyrrolidine-l- carboxylate as a yellow oil (10 g, 84%). LCMS (ESI, m/z): 263 [M+H]+.
302
144628010 vl Step 2. trans Benzyl 3«azido-4-fl oropyrroIidme-l-carhoxyIate
[00609] Into a 500-mL 3 -necked round-bottom flask was placed trans benzyl 3-azido-4- hydroxypyrrolidine-l-carboxylate (5.0 g, 19.06 mrnol) and dichlorom ethane (150 mL). Then DAST (9.6 g, 59.29 mmol) was added at -78 °C. The resulting solution was stirred for 1 h at - 78 °C in a liquid nitrogen bath. The resulting solution was allowed to react, with stirring, for an additional 14 h at room temperature. The reaction was then quenched by the addition of methanol (50 mL). The resulting mixture was concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 : 10) to afford trans benzyl 3-azido-4-fluoropyrrolidine-l-carboxylate as yellow oil (1.8 g, 36%). LCMS (ESI, m/z): 265 | \! ! ! j .
Step 3. trans Benzyl 3-amino-4-fluoropyrrolidine-l-carboxylate
[00610] Into a 25-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed trans benzyl 3-azido-4-fluoropyrrolidine-l-carboxylate (300 mg, 1.14 mmol), PPhi (890 mg, 3.40 mmol), tetrahydrofuran (5 mL) and water (1 mL). The resulting solution was stirred for 2 h at 70 °C in an oil bath under an atmophere of nitrogen. The resulting mixture was concentrated under vacuum and the residue diluted with EtOAc (100 mL) and washed with sat. aq. citric acid solution (2 x 100 mL). The combined aqueous extracts were washed with EtOAc (3 x 50 mL). The pH of the aqueous phase was adjusted to 8 with sat, aq. K2CO3 solution. The resulting mixture was extracted with DCM (4x 100 mL). The combined organic extracts were washed with brine (100 mL), dried over NaiSO-j, filtered, and concentrated under vacuum to afford trans benzyl 3-amino-4-fluoropyrrolidine-l-carboxylate as colorless oil (216 mg, crude). LCMS (ESI, m/z): 239 [M+H]+.
Step 4. trans Benzyl 3-((tert-butoxycarbonyl)amino)-4-fluoropyrrolidine-l-carboxylate
[00611] Into a 25-mL round-bottom flask, was placed trans benzyl 3-amino-4- fluoropyrrolidine-l-carboxylate (216 mg, 0.91 mmol), di-tert-butyl dicarbonate (218 mg, 1,00 mmol), water (1 ml.) and THF (5 mL). The resulting solution was stirred for 14 h at room temperature. The reaction was then quenched by the addition of 30 mL of water. The resulting solution was extracted with 3 x 15 ml, of ethyl acetate. The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :8)
303
144628010 vl to afford trans benzyl 3-((tert-butoxycarbonyl)amino)-4-fluoropyrrolidine-l -carboxylate as a colorless oil (260 mg, 85%), LCMS (ESI, m/z): 339 Γ\ί · H |
Step 5. tram Benzyl 3-((tert-butoxycarbonyl)(methyl)amino)-4-fluoropyrrolidine-l- carboxylate
[00612] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed trans benzyl 3-((tert-butoxycarbonyl)amino)-4-fluoropyrrolidine-l - carboxylate (2.00 g, 5.91 mmol), THF (20 mL), sodium hydride (591.1 mg, 14.78 mmol, 60% dispersion in oil) and Oil (1.25 g, 8.87 mmol). The resulting solution was stirred for 4 h at room temperature under an atmophere of nitrogen, and then poured into 20 mL of water/ice. The resulting mixture was extracted with 3 x 20 mL of dichloromethane, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :5) to afford trans benzyl-3-((tert-butoxycarbony[)(rnethyl)aniino)-4-f]uoropyrroiidine-l -carboxylate as yellow oil (1.3 g, 62%). LCMS (ESI, m/z): 353 [M+Hf.
Step 6, trans tert-Butyl (4-fluoropyrrolidin-3-yl)(methyl)carbamate
[00613] Into a 50-mL round-bottom flask fitted with a hydrogen balloon was placed trans benzyl-3-((tert-butoxycarbonyl)(methyl)amino)-4-fluoropyrrolidine-l-carboxylate (1.3 g, 3.69 mmol), 10% palladium on carbon (200 mg) and methanol (20 mL). The resulting solution was stirred for 2 h at room temperature under hydrogen. The solids were filtered out. The filtrate was concentrated under vacuum to afford trans te -butyl (4-fluoropyrrolidin-3-yl)(methyl)carbamate as colorless oil (850 mg crude), LCMS (ESI, m/z): 219 [M+H]~.
Step 7. trans tert-Butyl (l-(4-(2-(((benzyloxy)carbonyI)amino)ethyI)-2,5-difluorophenyl)-4- fluoropyrroIidin-3-yI)(methyl)carbamate
[00614] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed trans tert- ut l (4-fiuoropyrrolidin-3-yl)(methyl)carbarnate (450.0 mg, 2.06 mmol), benzyl (4-bromo-2,5-difluorophenethyl)carbamate (763.2 mg, 2.06 mmol), Pd(OAc)2 (46.3 mg, 0.21 mmol), XPhos (98.3 mg, 0.21 mmol), CS2CO3 (2.02 g, 6.18 mmol) and toluene (20 mL). The resulting solution was stirred for 4 h at 80 °C in an oil bath under an atmosphere of nitrogen. The resulting mixture was cooled to room temperature and quenched by the addition of
304
144628010 vl 20 mL of water. The resulting solution was extracted with 3 x 20 mL of dichloromethane, washed with 20 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 :6) to afford trans tert-butyl (l-(4-(2- (((benzyloxy)carbonyl)amino)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yi)(methyl)carbamate as a yellow oil (480 mg, 46%). LCMS (ESI, m/z) 508 [M+H]+.
Step 8. trans feri-Bnty! (l-(4-(2-aminoethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3- yl)(methyl)carbamate
[00615] Into a 50-mL round-bottom flask fitted with a hydrogen balloon, was placed trans tert-butyl (l-(4-(2-(((benzyIoxy)caAonyl)amino)ethyl)-2,5-difluorophenyl)-4-fluoropyn-oli yl)(methyl)carbamate (480 mg, 0,95 mmol), 10 % palladium on carbon (100 mg) and methanol (20 mL). The resulting solution was stirred for 2 h at room temperature under hydrogen. The solids were filtered out. The filtrate was concentrated under vacuum to afford tram tert-butyl (1- (4-(2-aminoethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyl)carbam as a brown oil (290 mg crude). LCMS (ESI, m/z): 374 [ M ! I | .
Step 9. ferf-Butyl ((3j¾,4 ?)-l-(4-(2-(3-amino-6-methylthieno[2,3-ft]pyridine-2- carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyl)carbamate and feri-Butyl ((3iS',4)S)-l-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxaniido)ethyl)- 2,5-difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyI)carbamate
[00616] Into a 50-mL round-bottom flask, was placed trans tert-butyl (l-(4-(2-aminoethyl)- 2,5-difluorophenyl)-4-fluoropyrrolidin-3-yl)(methyl)carbamate (280 mg, 0.75 mmol), dichloromethane (5 mL), 3-amino-6-methylthieno[2,3-/)]pyridine-2-carboxylic acid (156 mg, 0.75 mmol), EDCI (187 mg, 0,97 mmol), HOBt (122 mg, 0.90 mmol) and DIEA (194 mg, 1.50 mmol). The resulting solution was stirred for 2 h at 40 °C in an oil bath, and then quenched by the addition of 10 mL of water. The resulting solution was extracted with 3 x 10 mL of ethyl acetate. The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1 : 1). The racemate (260 rag) was separated by Chiral-Prep-HPLC using the following conditions (SHTMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 30.0%, Total Flow: 20
305
144628010 vl mL/min, Phase A: Hexanes-HPLC, Phase B: Ethanol-HPLC, Column Name: CHIRALCEL OJ- H, Length: 250 mm, internal Diameter: 20 mm, Particle Size: 5μηι, Column Temp: 20 °C, PDA Model : SPD-M20A, Wavelength: from 190 nm to 500 nm. This resulted in the following: Step 9, Enaatiomer A: 1st eluting peak (retention time = 8.19 min, 50 mg, 12% yield, yellow solid) assigned as tert-butyl ((3R,4R)- 1 -(4-(2-(3-amino-6-methylthieno[2,3-£]pyridine-2- carboxaniido)ethyl)-2,5-dil1uoroph LCMS (ESI. m/z): 564 [M+H]+; and Step 9, Enantiomer B: 2nd eluting peak (retention time = 11.8 min, 45 mg, 1 1 % yield, yellow solid) assigned as tert-butyl ((3S,45)-l -(4-(2-(3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyrrol^ yl)(rnethyi)carbamate. LCMS (ESI, m/z): 564 [M+H]+;
Step JO. 3-Amino-N-(2,5-difluoro-4-((3 f,4i?)-3-fluoro-4-(methylaraino)pyrrolidin-l- yl)phenethyI)-6-methyltliieno[2,3-6Jpyridine-2-carboxamide
[00617] Into a 10-mL round-bottom flask, was placed tert-butyl ((3R,4R)-l-(4-(2-(3-amino-6- methylthieno 2,3-6]pyridine-2-carboxamido)ethyl)-2,5-difluorophenyl)-4-fluoropyiTolidi yl)(methyl)carbamate (Step 9, Enantiomer A) (30 mg, 0.05 mmol), dichloromethane (2 mL) and TEA (0.5 mL). The resulting solution was stirred for 1 h at room temperature. The resulting mixture was concentrated under vacuum and the residue was diluted with 3 mL of water. The pH value of the solution was adjusted to 8 with ammonia. The resulting mixture was extracted with 3 x 10 mL ethyl acetate. The organic layers were combined, washed with 10 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum, The residue was purified by Prep-HPLC using the following conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 40.0%, End Cone, of Pump B: 43 ,0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (10 mmoi/L NH4HCO3), Phase B: MeCN-HPLC, Column Name: XB ridge Prep C18 OBD Column, Length: 150 mm, Internal Diameter: 19 mm, Particle Size: 5μτη, Aperture Size: 130 A, Column Temp: 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded 3-amino-N-(2,5-difluoro-4- ((3R,4R)-3-fluoro-4-(methylamino)pyrrol^
carboxamide as an off-white solid (6.5 mg, 26%). LCMS (ESI, m/z): 464 j \l \ \ [ [H~NMR (300 MHz, Methanol-^) 0 ppm 8.15 (d, J = 8.4 Hz, 1H), 7.26 (d, ,/ = 8.4 Hz, 1H), 6.95 - 6.91 (m,
306
144628010 vl 1H), 6.48 - 6.42 (m. i l k 5.12 - 4.94 (m, IH), 3.92 - 3.61 (ra, 2H), 3.61 ·■■■ 3.32 (m. 4! I ). 3.28■■■· 3.16 (m, 1H), 2.78 (S, J 7.2 Hz, 2H), 2.60 (s, 3H), 2.42 (s, 3H),
Step 10. 3-Amino-N-(2,5-difluoro-4-((3»S',4A,)-3-fluoro-4-(methylamino)pyrrolidin-l- yl)phenethyl)-6-inethylthieno[2,3-6]pyridine-2-carboxamide
[00618] The same synthetic procedure described to prepare 3-amino-/V-(2,5-difluoro-4- ((3R,4R)-3-fluoro-4-(methylaraino)pyrrolidin-l -yl)phenethyl)-6-methylthieno[2,3-/»]pyridi carboxamide was applied to Step 9, Enantiomer B except using the following Prep-HPLC conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 30.0%, End Cone, of Pump B: 36.0% Total Flow: 20 mL/min, Time: 8 min, Phase A: Water (10 rnmol NE HCOs), Phase B: MeCN-HPLC, Column Name: XBridge BEH C18
OBD Column, Length 150 mm, Internal Diameter: 19 mm, Particle Size: 5 μιη, Aperture Size; 130 A, Column Temp 25 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded -amino-iV-(2,5-diiluoro-4-((3.S,46)-3-fluoro-4-(meihylamino)pynOlidin-l - yl)phenethyl)-6-methylthieno 2,3-6]pyridine-2-carboxamide as an off-white solid (1.7 nig, 7%), I. CM (ESI, m/z): 464 [M+Hf, ¾-NM (300 MHz, Methanol-^) δ ppm 8.15 (d, J = 8.4 Hz, I H), 7.27 (d, J = 8.4 Hz, i l l), 6.95 - 6.88 (m, 1 H), 6.48 - 6,42 (m. 1 1 1 ), 5, 19 - 4.90 (m, 1H), 3.88 - 3.62 (m, 2H), 3.60 - 3.29 (m, 4H), 3.18 - 3.15 (m, IH), 2.78 (t, J = 12 Hz, 2H), 2.60 (s, 3H), 2.41 (s, 3H).
Example 112 (1-215). 3-Amino-N-(2-(5-chloro-6-(piperazin-l-yl)pyridin-3-yl)ethyl)-6- methyiihieno[2,3-ft]pyridi e-2-carboxamide
Figure imgf000308_0001
Siep 1
Ste 2
Figure imgf000308_0002
Step 4
Figure imgf000308_0003
Step 5 e' N
307
144628010 vl Step 1. iert~Buty\ 4-(5-broiiio-3-chloropyridin-2-yl)piperazine-l-carboxylate
[00619] Into a 250-mL round-bottom flask, was placed 5-bromo-2,3-dichloropyridine (5.0 g, 22.04 mmol), DMA (50 mL), potassium carbonate (9.16 g, 66.28 mmol), and ler l-buty\ piperazine-l-carboxylate (4.53 g, 24.32 mmol). The resulting solution was stirred overnight at 100 °C in an oil bath. The resulting solution was quenched with 200 mL of water and extracted with 3 x 250 mL of ethyl acetate. The organic layers were combined, washed with 3 x 750 mL of brine, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eiuted with ethyl acetate/petroleum ether (1/1) to afford /erf-butyl 4-(5- bromo-3-chloropyridin-2-yl)piperazine-l-carboxylate as colorless oil (3.2 g, 39%). LCMS (ESI, m/z): 376, 378 [M+Bf,
Step 2. terf-Butyl 4-(5-(2-(((benzyloxy)carbonyl)amino)ethyl)-3-chIoropyridin-2- yI)piperazine-l-carboxylate
[00620] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl 4-(5-bromo-3-chloropyridin-2-yl)piperazine-l-carboxylate (1.5 g, 3.98 mmol), toluene/water (15 mL/5 mL), CS2CO3 (3.1 g, 9.51 mmol), Pd(dppf)Ch (300 mg, 0.41 mmol), and potassium (2-(benzyioxycarbonylaniiiio)eihyi) trifluoroborate (1.63 g, 5.72 mmol). The resulting solution was stirred overnight at 100 °C in an oil bath. The resulting mixture was cooled to room temperature and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eiuted with ethyl acetate/petroleum ether (1/1) to afford tert-butyl 4-(5-(2-(((benzyloxy)carbonyl)amino)eihyl)-3-chloropyridin-2-yl)piperazine- 1-carboxylate as colorless oil (800 mg, 42%). LCMS (ESI, m/z): 475 { M i l l .
Step 3. ferf-Butyl 4-(5-(2-aminoethyl)-3-chloropyridin-2-yl)piperazine-l-carboxylate
[00621] Into a 50-mL round-bottom flask, was placed tert-butyl 4-(5-(2- (((benzy!oxy)caAonyl)amino)e l)-3-cMoropyridin-2-yl)piperazine-l- (300 mg,
0.63 mmol) and a solution of potassium hydroxide (40% aq.)/methanol (6 niL/6 mL). The resulting solution was stirred for 4 h at 80 °C in an oil bath. The resulting mixture was concentrated under vacuum and diluted with 5 mL of water. The resulting solution was extracted with 3 x 5 mL of dichlorom ethane. The organic layers were combined and concentrated under vacuum to afford tert-butyl 4-(5-(2-aminoethyl)-3-chloropyridin-2-yl)piperazine-l-carboxylate as a yellow solid (200 mg crude). LCMS (ESI, m/z): 341 [\! l !j
308
144628010 vl Step 4. iert~Buty\ 4-(5-(2-(3-amino-6-methylthieno 2 -6 yridine-2-carboxamido)ethyl)-3- chloropyridin-2-yl)piperazine-l-carboxylate
[00622] Into a 50-mL round-bottom flask, was placed iert-hutyl 4-(5-(2-aminoethyl)-3- chloropyridin-2-yl)piperazine-l-carboxylate (100 rag, 0.29 mmol), DMF (3 mL), DIEA (129 mg, 1.00 mmol), HATU (123 mg, 0.32 mmol), and 3-amino-6-methylthieno[2,3-b]pyridine-2- carboxylic acid (62 mg, 0.30 mmol). The resulting solution was stirred for 2 h at room temperature. The resulting solution was diluted with 9 mL of water and extracted with 3 x 12 mL of ethyl acetate. The organic layers were combined, washed with 3 x 36 mL of brine, and concentrated under vacuum. The crude product was purified via silica gel column chromatography and eluted with ethyl acetate/petroleum ether (1/1) to afford ferf-butyl 4-(5-(2- (3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-3-chloropyridin-2-yl)piperazine- 1-carboxylate as a yellow solid (60 mg, 39%). LCMS (ESI, m/z): 531 i \! I I ! .
Step 5. 3-Amino-N-(2-(5-chloro-6-(piperazin-l-yi)pyridin-3-yi)ethyl)-6-methylthieno[23- 6]pyridine-2-carboxaniide
[00623] Into a 50-mL round-bottom flask, was placed fert-butyl 4-(5-(2-(3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)-3-chloropyridin-2-yl)piperazine-l- carboxylate (60 mg, 0.11 mmol) and HCl/dioxane (4M, 5 mL). The resulting solution was stirred for 2 h at room temperature. The resulting mixture was concentrated under vacuum. The crude product was purified by Prep-HPLC using the following conditions: Column: X Bridge CI 8, 19x150 mm, 5 pirn; Mobile Phases: water (10 mM NH4HCO3 and 0.05% ammonia) and CH3CN; Gradient: 20% to 60% in 8 min; Flow rate: 15 mL/min; Detector, 254 nm. This afforded 3- amino-N-(2-(5-chloro-6-(piperazin-l-yl)pyridin-3-yl)ethyl)-6-methylthieno[2,3-6]pyridine-2- carboxamide as a light yellow solid (11.7 mg, 24%). LCMS (ESI, m/z): 431 [M+H]+. ¾- M (300 MHz, DMSO-t¾) δ pm 8.30 (d, ./= 8.4 Hz, 1H), 8.07 (s, i l l ). 7.80 - 7.69 (m, 21 1). 7.31 (d, J 8.4 Hz, 1H), 7. 12 (br s, 2H), 3.45 - 3.39 (m, 2H), 3.15 - 3, 13 (rri, 4H), 2,89 - 2.70 (in, 6H), 2.58 (s, 3H).
309
144628010 vl Examples 113- A (1-218) and 113-B (1-21.9). (S)-N-(4-((5-oxa-2-azaspiro[3.4]octan-" yl)oxy)phenethyl)-3-amino-6-methylthieno[2,3-i> pyridine-2-carboxamide and (R)~N-(4~{(i oxa-2-azaspiro[3.4]octan-7-yl)oxy)phenethyl)-3-amino-6-methylthieno[2,3-ft3pyridine-2- o
BO. "
„CC
Figure imgf000311_0001
Step 1. 3-(2-ethoxy-2-oxoethylidene)azetidine-l-carboxylate
[00624] into a 2-L round-bottom flask, was placed a solution of ethyl 2- (diethoxyphosphoryl)acetate (262 g, 1.17 rno3) in THF, This was followed by the addition of NaH (46.6 g, 1.17 mol, 60% dispersion in oil) at 0 °C over 1.5 h. After stirring for 30 min at RT, a solution of fert-butyl 3-oxoazetidine-l-carboxylate (100 g, 584 mmol) in THF (1.2 L) was added at 0 °C over 30 min. The resulting mixture was stirred for 40 min at RT. The reaction was then quenched by the addition of 50 mL of H2O and extracted with 3 x 500 mL of EtOAc. The
310
144628010 vl combined organic layers was dried over anhydrous Na SCk filtered, and concentrated under vacuum, The crude product was purified via silica gel column chromatography and eluted with EtO Ac/petroleum ether (1 :5) to afford fe/f-butyl 3-(2-ethoxy-2-oxoethylidene)azetidine~l- carboxylate as yellow oil (130 g, 92%). LCMS (ESI, m/z): 186 [ \f l f-/Bu ] .
Step 2, 2~(tert~Buty\) 8-ethyI 7-oxo-5-oxa-2-azaspiro[3.4]octane-2,8-dicarboxylate
[00625] Into a 2-L round-bottom flask purged and maintained with an inert atmosphere of N2 (g), was placed a solution of NaH (8 g, 200 mmol, 60% dispersion in oil) in ether (1 L). This was followed by the addition of methyl 2-hydroxyacetate (18 g, 200 mmol) dropwise with stirring at 0 °C over 30 min. After stirred for 30 min, the ether was removed in vacuo and to this was added a solution of ferf-butyl 3-(2-etiioxy-2-oxoethylidene)azetidine- 1 -carboxylate (40 g, 166 mmol) in DMSO (1 L) dropwise with stirring at RT over 30 min. The resulting solution was stirred overnight at RT. The pH value of the solution was adjusted to 4-5 with IN HCl and extracted with 3 x l L of ether. The organic phase washed with 3 x 500 raL of H2O and dried over anhydrous Na2S04, filtered, and concentrated under vacuum to afford 2-(fert-butyl) 8-ethyl 7- oxo-5-oxa-2-azaspiro[3.4]octane-2,8-dicarboxylate as yellow oil (46 g, 83%). LCMS (ESI, m/z): 300 [M+H]+.
Step 3. ferf-Butyl 7-oxo-5-oxa-2-azaspiro[3.4]octane-2-carboxylate
[00626] Into a 2-L 3 -necked round-bottom flask purged and maintained with an inert atmosphere of N2 (g), was placed a solution of 2-tert-buty\ 8-ethyl 7-oxo-5-oxa-2- azaspiro[3.4]octane-2,8-dicarboxylate (46 g, 138 mmol) and NaCl (16.7 g, 286 mmol) in DMSO/H2O (450 mL/45 mL). The resulting solution was stirred for 2 h at 120 °C in an oil bath. The reaction was then quenched by the addition of 40 mL of brine. The resulting solution was extracted with 5 x 1 L of ether. The combined organic layers was dried over anhydrous NaiSO-s, filtered, and concentrated under vacuum. The caide product was purified via silica gel column chromatography and eluted with EtO Ac/petroleum ether (1 :5) to afford ferf-butyl 7-oxo-5-oxa-2- azaspiro[3.4]octane-2-carboxyiate as a yellow solid (21.8 g, 69%). LCMS (ESI, m/z): 228 [ M I I ! .
Step 4. 7-hydroxy-5-oxa-2-azaspiro [3.4] octane-2-carboxy late
311
144628010 vl [00627] into a 250-mL round-bottom flask, was placed tert-butyi 7-oxo-5-oxa-2- azaspiro[3.4]octane-2-earboxylate (2 g, 8,80 mmol) and MeOH (15 mL), This was followed by the addition of NaBTL (1 g, 27.16 mmol) in portions. The resulting solution was stirred overnight at room temperature. The reaction was then quenched by the addition of 5 mL of water. The MeOH was removed under vacuum. The resulting solution was extracted with 3 x 10 ml. of EtOAc, The combined organic layers were dried over anhydrous Na2S04, filtered, and concentrated under vacuum to afford tert-butyl 7-hydroxy-5-oxa-2-azaspiro[3.4]oetane~2- carboxylate as a green liquid (2.01 g crude). LCMS (ESI, m/z): 230 [M+H]+.
Step 5. ferf-Butyl 7-(4-(2-(((benzyIoxy)carbonyl)amino)ethyl)phenoxy)-5-oxa-2- azaspiro [3,4] octane-2-carboxylate
[00628] Into a 25 -mL round-bottom flask was purged and maintained with an inert atmosphere of nitrogen, was placed benzyl (4-hydroxyphenethyl)carbamate (325 nig, 1.20 mmol), THF (6 mL), tert-butyl 7-hydroxy-5-oxa-2-azaspiro[3.4]octane-2-carboxylate (413 rag, 1.80 mmol) and triphenylphosphine (472 mg, 1.80 mmol). This was followed by the addition of diisopropyl azodicarboxylate (DIAD) (364 mg, 1.80 mmol) dropwise with stirring at 0 °C. The resulting solution was stirred for overnight at room temperature. The resulting mixture was poured into 20 mL of water and the resulting mixture was extracted with 3 x 40 mL of ethyl acetate. The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by silica gel column chromatography and eluting with ethyl acetate/petroleum ether (2: 1.) to afford tert-butyl 7-(4-(2- (((benzy oxy)carbonyl)amino)ethyi)phenoxy)-5-oxa-2-azaspiro[3,4]octane-2-carboxy as a white solid (410 rag , 71 %). LCMS (ESI, m/z): 483 |_M · l lj
Step 6. teri-Butyl 7-(4-(2-aminoethyl)phenoxy)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate
[00629] Into a 25 -mL round-bottom flask equipped with hydrogen balloon, was placed tert- butyl 7-(4-(2-(((benzyloxy)carbonyl)amino)ethyl)phenoxy)-5-oxa-2-azaspiro[3.4]octane-2- carboxylate (410 mg, 0.85 mmol), methanol (12 mL) and 10% Pd/C (60 mg). The resulting mixture was stirred for 3 h at room temperature under hydrogen. The solids were filtered out and the filtrate was concentrated under vacuum to afford tert-butyl 7-(4-(2-aminoethyl)phenoxy)-5- oxa-2-azaspiro[3.4]octane-2-carboxyIate as colorless oil (244 mg, crude). LCMS (ESI, m/z): 349 I i i ί !~
312
144628010 vl Step 7. iert~Buty\ (5)-7-(4-(2-(3-ainino-6-methylthieno[2,3-6|pyridine-2- carboxamido)ethyl)phenoxy)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate and if/f- Butyl (if)- 7-(4-(2-(3-amino-6-methylthieno[2,3-6]pyridine-2-carboxamido)ethyl)phenoxy)-5-oxa-2- azaspiro [3,4] octane~2~carboxylate
[00630] Into a 25-mL round-bottom flask, was placed tert-butyl 7-(4-(2- aminoethyl)phenoxy)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate (132 mg, 0.38 mmol), DMF (4 mL), 3-amino-6-methylthieno[2,3-Z>]pyridine-2-carboxylic acid (79 mg, 0.38 mmol), DIEA (155 mg, 1.20 mmol), EDCI (150 mg, 0.78 mmol) and HOBt (133 mg, 0.98 mmol), The resulting solution was stirred for overnight at room temperature. The resulting solution was poured into 30 mL of water and the mixture was extracted with 3 x 20 mL of ethyl acetate. The organic layers were combined, washed with 3 x 20 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by silica gel column chromatography and eluted with ethyl acetate/petroleum ether (3 : 1) to afford tert-but l 7-(4-(2-(3-amino-6- methyltMeno[2,3-d]pyridine-2~caAoxamido)ethyl)phenoxy)-5-oxa-2-azaspiro[3
carboxylate as a light brown solid (110 mg, 54%). The racemate was separated by Chiral-Prep- HPLC using the following conditions (SH ADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 50,0%, Total Flow: 20 mL/min, Phase A: Hex-HPLC, Phase B: EtOH-HPLC, Column Name: CHIRALPAK IF, Length: 25 mm, Internal Diameter: 2 cm, Particle Size: 5 μτη, Column Temp: 20 °C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This resulted in the following: Step 7, Enantiomer A: 1 si eluting peak (retention time = 14.4 min, 40 mg, 20% yield, white solid) assigned as tert-butyl (S)-7-(4-(2-(3-amino-6- methylthieno[2,3~d]pyridine-2-carboxamido)ethyl)phenoxy)-5-oxa-2-az
carboxylate. LCMS (ESI, m/z): 539 [M+Ffp and Step 7, Enantiomer B: 2nd eluting peak (retention time = 19,3 min, 39 mg, 19% yield, white solid) assigned as tert-butyl (R)-7-(4-(2-(3- amino-6-methylthieno[2,3- ?]pyridine-2-carboxamido)ethyl)phenoxy)-5-oxa-2- azaspiro[3.4]octane-2-carboxylate. LCMS (ESI, m/z): 539 \ % \
Step 8, ( )-iV-(4-((5~oxa-2-azaspiro [3.4] octan-7-yl)oxy)phenethyI)-3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamide
[00631] Into a 8-mL vial, was placed tert-butyl (S)-7-(4-(2-(3-amino-6-methylthieno[2,3- ft]pyridine-2-carboxamido)ethyl)phenoxy)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate (Step 7,
313
144628010 vl Enantiomer A) (45 mg, 0.08 mmol), dichloromethane (2.1 mL) and trifluoroacetic acid (0.7 niL). The mixture was stirred for 1 h at room temperature. The solvent was evaporated under vacuum and then the residue was dissolved in methanol (1 mL), This was followed by the addition of a solution of NH3 in MeOH (7M, 5 mL) dropwise with stirring. The resulting solution was stirred for 0.5 h at room temperature, and then concentrated under vacuum. The crude product was purified by Prep-HPLC using the following conditions (SEQMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 25.0%, End Cone, of Pump B: 45.0% Total Flow: 20 mL/min, Time: 7 min, Phase A: Water (0.1% formic acid), Phase B: MeCN-HPLC, Column Name: XB ridge Prep C18 OBD Column, Length: 250 mm, Internal Diameter: 19 mm, Particle Size: 5 μιη, Aperture Size: 100 A, Column Temp: 25°C, PDA Model: SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded (tS)-N-(4-((5-oxa-2- azaspiro[3.4]octan-7-yl)oxy)phenethyl)-3-amino-6-methylthieno[2,3-0]pyridine-2-carboxamide as a green solid (16 mg, 44%). LCMS (ESI, m/z): 439 [M+Fff . Ή-NMR (300 MHz, DMSO-<&) δ ppm 8.36 (s, 1H), 8.31 (d, ,/ = 8.1 Hz, IH), 7.72 (t, J = 5.4 Hz, 1H), 7.31 (d, J = 8.4 Hz, 1H), 7.17 - 7.12 (m, 4H), 6.84 (m, ./ ··· 8.4 Hz 2H), 5.01 ■■■■ 5.03 (m, IH), 3,98 ·■■■ 4,03 (m, IH), 3.72 - 3.91 (m, 5H), 3.42 - 3.35 (m, 2H), 2.78 - 2.73 (m, 2H), 2.58 (s, 3H), 2.45 - 2.27 (m, 2H).
Step 8, (l?)-N-(4-((5-oxa-2-azaspiro [3.4] octan-7-yl)oxy)phenethyl)-3-amino-6- methylthieno[2,3-6]pyridine-2-carboxamide
[00632] The same synthetic procedure described to prepare (S)-N-(4-((5-oxa-2- azaspiro[3.4]octan-7-yl)oxy)phenethyl)-3-amino-6-memy^
was applied to Step 7, Enantiomer B to afford (R)-Ar-(4-((5-oxa-2-azaspiro[3.4]octan-7- yl)oxy)phenethyl)-3-amino-6-methylthieno[2,3-Z>]pyridine-2-carboxamide as a green solid (18.8 mg, 51%). LCMS (ESI, m/z): 439 | \Ι · Π | \ Ί !-Wi (300 MHz, DM Si δ ppm 8,34 (s, I H), 8.29 (d, J = 8.4 Hz, IH), 7.70 (t, J = 5.7 Hz, IH), 7.30 (d, J = 8.4 Hz, IH), 7.17 - 7.12 (m, 4H), 6.84 (d, ,/ 8.7 Hz, 2H), 5.01 - 5,03 (m, I H), 3,98 - 4,03 (m. I H), 3,67 - 3 ,89 (m, 5H), 3 ,42 - 3.35 (m, 2H), 2.78 - 2.73 (m, 2H), 2.58 (s, 3H), 2.43 - 2.29 (m, 2H).
314
144628010 vl Example 114 (1-220). 3-Amino-6-cyano-A7-(2,5-diiluoro-4-(piperazin-I- yl)phenethyI)thienof2,3-6]pyridine-2-carboxamide
Figure imgf000316_0001
Figure imgf000316_0002
Step 1, tert~B\ityl 4-(4-(2-(3-amino-6-chlorothieno [2,3-6] pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)piperazine-l-carboxyiate
[00633] Into a 50-mL round-bottom flask, was placed a solution of 3-amino-6- chlorothieno[2,3-6]pyridine-2-carboxylic acid (Intermediate 21) (300 mg, 1 .31 mmol) in DMF (3 mL), HATU (608 mg, 1.60 mmol), DIEA (255 mg, 1.97 mmol) and tot-butyl 4-(4-(2- aminoethyl)-2,5~difluorophenyl)piperazine-l-carboxylate (Intermediate 49) (403 mg, 1.18 mmol). The resulting solution was stirred for 1 h at room temperature. The reaction was then quenched by the addition of 10 mL of water. The resulting mixture was extracted with 3 x 10 mL of ethyl acetate. The organic layers were combined, washed with 30 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 1) to afford tert-buiyl 4-(4-(2-(3-amino-6-chlorothieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)piperazine-l.-carboxylate as a yellow solid (300 mg, 41%). LCMS (ESI, m/z): 552, 554 [M+Hf .
Step 2, tert-But l 4-(4-(2-(3-amino-6-cyanothieno[2,3-6]pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)piperazine-l-carboxylate
[00634] Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed tot-butyl 4-(4-(2-(3-amino-6-chlorothieno[2,3-6]pyridine-2- carboxamido)ethyi)-2,5-dif]uorophenyl)piperazine-l-carboxyiate (240 mg, 0.43 mmol), Zn(CN)2 (80 mg, 0.68 mmol), Zn (6 mg, 0.09 mmol), 2nd Generation XPhos Precatalyst (XPhos Pd G2)
315
144628010 vl (36 mg, 0.046 ramol) and dioxane (4 mL). The resulting solution was stirred overnight at 90 °C under an atmophere of nitrogen. The resulting mixture was coo! to room temperature. The reaction was then quenched by the addition of 20 mL of water. The resulting solution was extracted with 3 x 20 mL of ethyl acetate. The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by silica gel chromatography and eluted with ethyl acetate/petroleum ether (1 : 1) to afford fert-butyi 4-(4-(2-(3-amino-6-cyanothieno[2,3-^]pyridine-2-carboxamido)ethyl)-2,5- difluorophenyl)piperazine-l-carboxylate as a yellow solid (150 mg, 64%). LCMS (ESI, m/z): 543 I f ϊ ϊ
Step 3, 3-Amino-6-cyano-N-(2,5-difluoro-4-(piperazin-l-yl)phenethyl)thieno[2,3- 6]pyridine-2-carboxamide
[00635] Into a 50-mL round-bottom flask, was placed tert-butyl 4-(4-(2-(3-amino-6- cyanothieno[2,3-^]pyridine-2-carboxamido)ethyi)-2,5-dif!uorophenyl)piperazin
(60 mg, 0.11 mmoi), DCM (1 mL) and TFA (0.5 mL). The resulting solution was stirred for 0.5 h at room temperature and concentrated under vacuum. The pH value of the residue was adjusted to 8 with a solution of ML in MeOH (7M). The resulting mixture was extracted with 3 x 10 mL ethyl acetate. The organic layers were combined, washed with 10 mL of brine, dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum. The residue was purified by Prep-HPLC using the following conditions (SHIMADZU LC-20AD): LC parameters: Pump Mode: Binary gradient, Start Cone, of Pump B: 25.0%, End Cone, of Pump B: 80,0% Total Flow: 15 mL/niin, Time: 9 min, Phase A: Water (10 mM NH4HCO3), Phase B: MeCN-HPLC, Column Name: XBridge BEH C18 OBD Column, Length 150 mm, Internal Diameter: 19 mm, Particle Size; 5 pm, Aperture Size: 130 A, Column Temp; 25 °C, PDA Model : SPD-M20A, Wavelength: from 190 nm to 500 nm. This afforded 3-amino-6-cyano-jV-(2,5-difluoro-4- (piperazin-l-yl)phenethyl)thieno[2,3-5]pyridine-2-carboxamide as a yellow solid (2.9 mg, 6%). LCMS (ESI, m/z): 443 [M+H]+. 'H-NMR (300 MHz, Methanol-^) δ ppm 8.47 (d, J = 8.4 Hz, 1H), 7.83 (d, J ------ 8. 1 Hz, 1H), 7,04 - 6.97 (m, I ! ! }, 6.79 - 6.73 (m, 1H), 3.57 - 3.52 (m, 2H),
3, 13 - 2.92 (m, 8H), 2.90 - 2.85 (m, 21 1 ).
316
144628010 vl Examples 115-A (1-221) and 115-B (1-222). (tS)-3-Amino-6-(l-hydroxyethyl)-N (piperazin-l-yl)phenethyl)thieno[2,3-6]pyridine-2-carboxamide and (i?)-3-A
hydroxyethyl)-N-(4-(piperazin-l-yI)phenethyl)thieno[2,3-¾]pyridine-2-carboxamide
Figure imgf000318_0001
Enantiomer A
TFA, CH2CI2
Ste S Step 6
Figure imgf000318_0002
Example 115-A (1-221 ) Example 115-B (i-222)
Step 1. Methy! 3-amino-6-(dimethoxymethyl)thieno[2,3-6]pyridine-2-carboxylate
[00636] Into a 50-mL 3 -necked round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6-(dimethoxymethyl)-2-sulfanylpyridine-3-carbonitrile (500 nig, 2.38 mmol), THF (10 mL), methyl 2-chloroacetate (310 mg, 2.86 mmol), and DBU (1.09 g, 7, 16 mmol). The resulting solution was stirred overnight at room temperature. The resulting mixture was concentrated under vacuum. The residue was purified by silica gel chromatography and eiuted with ethyl acetate/petroleum ether (3 :7) to afford methyl 3-amino-6- (dimethoxymethyl)thieno[2,3-&]pyridine-2-carboxylate as a yellow solid (852 mg, crude). LCMS (ESL m/z): 283[M+H]+.
317
144628010 vl Step 2. Methyl 3-amino-6-fori-iyIthieno[2,3-&]pyridine-2-carboxyIate
[00637] Into a 100-mL round-bottom flask, was placed methyl 3-amino-6- (dimethoxymethyl)thieno[2,3-6]pyridine-2-carboxylate (842.5 mg, 2.98 mmol), THF (10 mL), and aq. HC1 (IN, 30 mL). The resulting solution was stirred overnight at 40 °C. The resulting mixture was concentrated under vacuum. The resulting solution was extracted with 2x200 ml, of ethyl acetate. The organic layers were combined and washed with 50 mL of sat. aq. sodium bicarbonate solution and 50 mL of brine. The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated under vacuum to afford methyl 3-amino-6-formylthieno[2,3- 6]pyridine-2-carboxylate as a yellow solid (870 mg, crude). lH-NMR (300 MHz, CDCh) 6 ppm 10.17 (s, I I I). 8, 13 - 8.11 (m, I ! I ), 8.00 - 7,97 (m, 11 1), 3.96 (s, 3H),
Step 3. Methyl 3-amino-6-(l-hydroxyethyl)thieno [2,3-6] pyridine-2-carboxylate
[00638] Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed methyl 3-amino-6-formylthieno[2,3-&]pyridine-2-carboxylate (870 mg, 3.68 mmol), THF (20 mL). This was followed by the addition of CHsMgBr (3M in diethyl ether) (12 mL) dropwise with stirring at 0 °C in 1 hr. The resulting solution was stirred overnight at room temperature. The reaction was then quenched by the addition of 20 mL of methanol . The resulting mixture was concentrated under vacuum. The residue was purified by silica gel chromatography and eiuted with dichloromethane/ethyl acetate (1 : 1) to afford methyl 3-amino-6- (l-hydroxyethyl)thieno[2,3-6]pyridine-2-carboxylate as a yellow solid (300 mg, 32%). lH-NMR (300 MHz, CDCh) δ ppm 7.93 id, J 8.4 Hz, 1H), 7.29 id, ./ 8,4 Hz, 1 1 1), 5.05 - 4.98 (m, H i).. 3.91 (s, 3H), 1.58 - 1.55 (m, 3H).
Step 4. 3-Amino-6-(l-hydroxyethyI)thieno[2,3-6]pyridine-2-carboxylic acid
[00639] into a 50-rnL round-bottom flask, was placed methyl 3-amino-6-(l- hydrox.yethyl)thi.eno[2,3-6]pyridine-2-carboxylate (350 mg, 1.39 mmol), THF (10 mL), water (3 mL), and LiOH (100 mg, 4.18 mmol). The resulting solution was stirred overnight at 60 °C. The reaction mixture was cooled to room temperature and concentrated under vacuum. The pH value of the solution was adjusted to 3 with IN HC1. The solids were collected by filtration to afford 3- amino-6-(l-hydroxyethyl)thieno[2,3-6]pyridine-2-carboxylic acid as a yellow solid (40 mg, 12%). LCMS (ESI, m/z): 239 ! M-H] .
318
144628010 vl Step 5. iert~Buty\ (A1)-4-(4-(2-(3-ami!io-6-(l-hydroxyethyI)i ieno|2,3- i]pyridiiie-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate and tert- iityl (i?)-4-(4-(2-(3-amino-6- (l-hydroxyethyl)t ieno[2,3-A]pyridine-2-carboxamido)ethyl)phenyI)piperazine-l- carboxylate
[00640] Into a 8-rnL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 3-amino-6-(l-hydroxyethyl)thieno[2,3-Z>]pyridine-2-carboxylic acid (28.4 rng, 0.12 mmol), tert-butyl 4-(4-(2-aminoethyl)phenyl)piperazine-l-carboxylate (43.7 mg, 0.14 mmol), HATU (68.4 mg, 0.18 mmol), DIEA (31 mg, 0.24 mmol), and THF (2 mL). The resulting solution was stirred overnight at room temperature. The resulting mixture was concentrated under vacuum. The crude product was purified by Prep-TLC with ethyl acetate to afford ferf-butyl 4-(4-(2-(3-amino-6-(l-hydroxyethyl)thieno[2,3-i]pyridine-2- carboxamido)emyl)phenyl)piperazine-l-carboxylate as a solid (20 mg, 32%). The racemate was separated by Chiral-Prep-HPLC using the following conditions (SHIMADZU LC-20AD): LC Parameters: Pump Mode, Binary gradient, start cone, of pump B, 40.0%, Total Flow:l mL/min; Phase A: n-Hexanes, Phase B: Isopropanol; Column Name; DAICEL C'HIRAL PAK AD-3; Length: 50 mm; Internal Diameter: 4.6 mm; Particle size: 3 μιη; Column Temp.: 25 °C; PDA Model ; SPD-M20A; Wavelength: from 190 nm to 500 nm. This resulted in the following: Step 5, Enantiomer A: 1st eluting peak (retention time :;= 4.0 rain, 60 mg, 50% yield, yellow solid) assigned as feri-butyl (S)-4-(4-(2-(3 -amino-6-( 1 -hydroxyethyl)thieno[2,3-^]pyiidine-2- carboxamido)ethyi)phenyl)piperazine-l-carboxylate. LCMS (ESI, m/z): 526 [M+H]+; and Step 5, Enantiomer B: 2nd eluting peak (retention time = 5.2 min, 50 mg, 50% yield, yellow solid) assigned as tert-butyl (R) -(4-(2-(3-amino-6-(l-hydroxyethyl)thierio[2,3-¾]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate. LCMS (ESI, m/z): 526 j ! - 1 1 1 .
Step 6. (A 3-Amino-6-(l-hydroxyethyl)-N-(4-(piperazin-l-yl)phenethyl)thieno[2,3- h\ pyr idine-2-carboxam ide
[00641] Into a 50-mL round-bottom flask, was placed ie/ -butyl (5)-4-(4-(2-(3-amino-6-(l- hydroxyethyl)thieno[2,3 -6]pyridine-2-carboxamido)ethyl)phenyl)piperazine- 1 -carboxylate (Step 5, Enantiomer A) (22 mg, 0,04 mmol), dichloromethane (2 mL), and TFA (0.4 mL). The resulting solution was stirred for 1 h at room temperature, and then concentrated under vacuum. The crude product was purified by Prep-HPLC using the following conditions; Column: X
319
144628010 vl Bridge C18, 19x150 mm, 5 μιη, mobile phase: water (10 mM NHHCCb, 0.05% ammonia) and CH3CN; Gradient: 35% to 45% in 8 min; Flow rate: 15 mL/min; Detector: 254 nm. This afforded ( )-3-amino-6-(l -hydroxy ethyl)-Ar-(4-(piperazin-l-yi)phenethyl)thieno[2,3-0]pyridi
carboxamide as a yellow solid (9 mg, 51%). LCMS (ESI, m/z): 426 j \f · ! ! ) ' . !H-NMR (300 MHz, Methanol-^;) 0 ppm 8.30 (d, J = 8.1 Hz, 1H), 7.57 (d, J = 8,4 Hz, 1H), 7.20 - 7.17 (m, 2H), 6.96 - 6.93 (m, 2H), 4.97 - 4.91 (ra, 1H), 3 ,59 - 3.51 (m, 2H), 3.17 - 3.12 (m, 4H). 3.08 - 2.93 (m, 4H), 2.89 - 2.80 (m. 2! !). 1.53 (d, 6.6 Hz, 3H).
Step 6, (j?)-3-Amino-6-(l-hydroxyethyl)-N-(4-(piperazin-l-yI)phenethyl)thieno[2,3- -2-ca
[00642] The same synthetic procedure described to prepare (S)-3-amino-6-(l -hydroxy ethyl)- N-(4-(piperazin-l-yl)phenethyl)thieno[2,3-i]pyridine-2-carboxamide was applied to Step 5, Enantiomer B to afford (R)-3-amino-6-(l -hydroxy ethyl)-Ar-(4-(piperazin-l- yl)pheneihyl)thieno[2,3 ]pyridine-2-carboxamide as a yellow solid (9 mg, 51%). LCMS (ESI, m/z): 426 [M+H]+. l i-W! (300 MHz, Methanoi-ώ) δ ppm 8.30 (d, 8.4 Hz, 1 1 1). 7.57 (d, J = 8.4 Hz, lH), 7.19 - 7.12 (m, 2H), 6.96 - 6.93 (m, 2H), 5.00 - 4.91(m, lH), 3.54 - 3.49 (m, 2H), 3.13 - 3.11 Cm. 4H), 3.10 - 2,93 (m, 4H), 2.85 - 2,80 (m, 21 1 ). 1 ,53 (d, J - 6.6 Hz, 3H).
Ex m e 116 (1-223). 3-Amino-7-hydroxy-N-(4-(piperazin-l-yl)phenethyI)-6,7-dihydro-5H- cyclopenta[6]thieno[3,2-e]pyridine-2-carboxamide
Figure imgf000321_0001
1 ) Na2C03, EtOH, 110 °C 2i NaOH, THF/MeOH
Step 5
Figure imgf000321_0002
320
144628010 vl Step 1. terf-Butyl 4-(4-(2-(2-(acetyIthio)acetainido)ethyl)phenyl)piperazine-l -carboxylate
[00643] A mixture of HATU (1.62 g, 4.26 mmol), DIEA (1.72 mL, 9.82 mmol), ferf-butyl 4- (4-(2-aminoethyI)phenyl)piperazine-l-carboxylate (1 g, 3 ,27 mmol), and 2-(acetylthio)acetic acid (0.338 mL, 3.27 mmol) in THF (20 mL) and DMF (40 mL) was stirred at RT overnight, and then concentrated under vacuum. The residue was taken up in EtOAc, and then washed with water and brine. The organic phase was dried and concentrated. The crude product was purified by silica gel chromatography and eluted with 10 to 100% EtOAc/Hexanes to afford fert-butyl 4- (4-(2-(2-(acetylthio)acetamido)ethyl)phenyl)piperazine-l -carboxylate (700 nig, 50,7%). LCMS (ESI, m/z): 220 [M+H . lH-NMR (300 MHz, CDCh) δ ppm 7.08 (d, J= 8.2 Hz, 2H), 6.87 (d, J = 8.2 Hz, 2H), 6, 14 (br s, 11 1), 3,56 (m, 4H), 3,48 (s, 2H), 3.41 - 3,47 (m, 21 1). 3,09 (m, 41 I), 2.80 (d, J= 0.9 Hz, 2H), 2.71 (m, 2H), 2.35 (s, 3H), 1.48 (s, 9H).
Step 2. teri-Biityl 4-(4-(2-(2-mercaptoacetamido)ethyl)phenyI)piperazine-l-carboxylate
[00644] A solution of ferf-butyl 4-(4-(2-(2-(acetylthio)acetamido)ethyl)phenyl)piperazine-l- carboxylate (620 mg, 1.47 mmol) in MeOH (20 mL) was bubbled with N?. for 15 min, and then ammonia (7 , 10 mL, 70 mmol) was added. The mixture was stirred under N?. gas for 2 h. The reaction mixture was concentrated under vacuum. The residue was taken up in sat, aq, XI ! iCi solution, extracted with EtOAc, dried and concentrated to afford fert-butyl 4-(4-(2-(2- mercaptoacetamido)ethyl)phenyl) piperazine-l-carboxylate (354 mg, 63,4%), LCMS (ESi, m/z): 380 [M+Hf. ! IAMR (300 MHz, CDCh) δ ppm 6.98 - 7.12 (d, J= 8.5 Hz, 2H), 6.80 (d, 7 - 8.5 Hz, 21 1 ). 6,48 (m, 1H), 3.39 - 3.63 (m, 61 1). 3.28 (s, 2H), 2.97 - 3.10 (m, 4H), 2.66 - 2.83 (m,2H), 1.41 (s, 9H).
Step 3. 2-Chloro-l-(λ1-o idan l)-6,7-dih dro-5/-r-lλ4-cycIopenta[6]pyridine-3-carbonitriIe
[00645] To a stirred solution of 2-chloro-6,7-dihydro-5H-cyclopenta[i]pyridine-3-carbonitrile
(600 mg, 3.36 mmol)) in acetic acid (10 mL) was added H2O2 (1.54 mL, 15.12 mmo!). The resultant mixture was heated at 80 °C for 16 h. The reaction mixture was then allowed to cool to room temperature and concentrated in vacuo. The residue was taken up in water (100 mL). The slightly acidic solution was neutralized by the careful addition of potassium carbonate (-1.0 g) and then extracted with chloroform (3 x 30 mL). The combined organic extracts were washed with water (3 x 15 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated in
321
144628010 vl vacuo to afford crude 2-chloro-l-( 1-oxidanyl)-6 -dihydro-5i/-l 4-cyclopenta[ ?]pyridine-3- carbonitrile as a white crystalline solid,
Step 4. 2-CMoro-3-cyano-6,7-dihydro-5H-cyclopeota[&Jp ridin-7-yI acetate
[00646] The crude 2-chloro-l.-( 1-oxidanyl)-6,7-dihydro-5H^-l 4-cyclopenta[i>]pyridine-3- carbonitrile from above was taken up in acetic anhydride (25 mL) and the resultant suspension was stirred at room temperature for 1 h and then heated at 100 °C for 4 h. The reaction mixture was then allowed to cool to room temperature and was concentrated in vacuo. The crude product was purified by silica gel chromatography and eluted with hexanes/ diethyl ether (1 : 1) to afford 2-chloro-3-cyano-6,7-dihydro-5H-cyclopenta[/»]pyridin-7-yl acetate (500 mg, 63% yield over 2 steps). LCMS (ESI, rri/z): 238 [ M \ \ \ . ¾~NMR (300 MHz, CDCh) 5 ppm 8,05 (s, IH), 6. 1 1 (dd, J = 7.3, 3.5 Hz, ! ! !}, 3.24 (dd, J = 8,8, 6.7 Hz, I I I). 3.08 (br dd, J= 8,9, 4,8 Hz, IH), 2,48 2.72 (m. IH), 2.27 (m,lH), 2.08 (s 3H).
Step 5. iert-B tyl 4-(4-(2-(3-amino-7-hydroxy-6,7-dihydro-5H-cyclopentai6]thieno[3,2- e]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylatelate
[00647] A mixture of Na2COs (134 mg, 1.27 mrao!), 2-chloro-3 -cyano-6, 7-dihydro-5H- cyclopenta[i]pyridin-7-yl acetate (100 mg, 0.423 mmo!), and tert-but l 4-(4-(2-(2- mercaptoacetamido)ethyl)phenyl)piperazine-l-carboxylate ( 160 mg, 0.423 mmol) in ethanol (2 mL) was stirred at 110 °C ( oil bath temperature) in a sealed tube. The reaction mixture was then cooled to RT and diluted with 15 mL of water. The resulting mixture was filtered, and the solid was purified by silica gel chromatography and eluted with 0 to 10% MeOH/DCM to afford tert- butyl 4-(4-(2-(7-acetoxy-3-amino-6,7-dihydro-5H"-cyclopenta[^]thieno[3,2-e]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (77 mg, 31.4%). To a solution of fer/-butyl 4-(4-(2-(7-acetoxy-3-amino-6,7-dihydro-5. /-cyclopenta[i]thieno[3,2-e]pyridine-2- carboxamido)ethyl)phenyl)piperazine-l-carboxylate (77 mg, 0.133 mmol) in 10 mL of MeOH and 10 mL of THF was added 10 mL of IN NaOH. The mixture was stirred at RT overnight, and then concentrated in vacuo. The residue was taken up in EtOAc, washed with brine, dried, filtered, and concentrated. The crude product was purified by silica gel chromatography and eluted with 20 to 100% EtOAc/Hexanes to afford ferf-butyl 4-(4-(2-(3-amino-7-hydroxy-6,7- dihydro-5H-cyclopenta[i]thieno[3,2-^]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l- carboxylate (37 mg, 51.7%). LCMS (ESI, ra/z): 538 I X! ! I f. Ί (AM (300 MHz, CDCh) δ
322
144628010 vl ppm 7.77 (s, 1H), 7.15 (d, J= 8.5 Hz, 2H), 6.89 (d, J= 8.5 Hz, 2! I). 6.01 (br s, 2H), 5.54 - 5.68 (m, 1H), 5,22 - 5,37 (m, 1H), 3.52 - 3.73 (m, 6H), 3. 10 (br s, 4H), 2.76 - 2.99 (m, 4H), 2.58 - 2.74 (m, 1H), 1.48 fs, 9H).
Step 6. 3-Amino-7-hydroxy-N-(4-(piperazin-l-yl)phenethyl)-6,7-dihydro-5H- cycIopenta[&]thieno[3,2-e]pyridine-2-carboxamide hydrochloride salt
[00648] To a solution of tert-butyl 4-(4-(2-(3-araino-7-hydroxy-6,7-dihydro-5H"- cyclopenta[i»]thieno[3,2-e]pyridine-2-carboxamido)ethyl)phenyl)piperazine-l-carboxylate (37 mg, 0,069 mmol) in methanol (10 mL) was added 2N HC1 (0,052 mL, 0.206 mmol) at RT, The mixture was stirred at RT overnight and concentrated. The residue was taken up in diethyl ether, filtered, and the solids dried to afford 3-amino-7-hydroxy-N-(4-(piperazin-l-yl)phenethyl)-6,7- dihydro-5H-cyclopenta[Z>]thieno [3,2-e]pyridine-2-carboxamide as the hydrochloride salt (29,2 mg). LCMS (ESI, m/z): 438 [M+Hf . ' l i-NMR (300 MHz, DMSO-^) δ ppm 9.07 (br s, 2H), 8.20 (s, 1H), 7,67 (br I . J = 5.3 Hz, 1 H), 7.06 (br d, ./ »- 8.5 Hz, 21 1 ). 6.87 (br d, J = 8.5 Hz, 2H), 4.95 (m, i l l), 3.56 - 3.71 (m, 1H), 3.30 (m, 2H), 3.1 1 (m, 4H), 2.85 - 3.05 (m, 2H), 2.56 - 2.84 (m, 3H), 2.21 - 2,39 (m, 2H), 1.83 (m, 2H).
Example 117: Biochemical Assay: Ubiquitin-Rhodamine 110 Assay for USP28 Activity.
[00649] The assay was performed in a final volume of 20 in assay buffer containing 20 mM Tris-HCi (pH 8.0, (1M Tris-HCl, pH 8.0 solution; Coming 46-031-CM)), 2 raM CaCh (1M Calcium Chloride solution; Sigma #21 114) 2 mM BME (2-Mercaptoethanol; Sigma 63689- 25ML-F), 0.01% Prionex (0.22 μΜ filtered, Sigma #G-0411), and 0.01% Triton X-100. Stock compound solutions were stored at. -20 °C as 10 mM in DM SO, Up to 1 month prior to the assay, 2 mM test compounds were pre-dispensed into assay plates (Black, low volume; Corning #3820) and froze at -20 °C, Prestamped assay plates were allowed to come to room temperature on the day of the assay. For the screen, 100 nL of 2 mM was pre-dispensed for a final screening concentration of 10 μΜ (DMSG(fc) = 0.5%). Enzyme (USP28, construct USP28 (USP28-5(1- 1077)-TEV-6*His; LifeSensors) concentration and incubation times were optimized for the maximal signal-to-background while maintaining initial velocity conditions at a fixed substrate concentration. The final concentration of the enzyme in the assay was 400 . Final substrate (Ub-Rhl lQ; Ubiquitin-Rhodamine 110, R&D Systems #U-555) concentration was 25 nM with [Ub-Rhl 10 ]«Km. 10 μ,Ε of 2x enzyme was added to assay plates (pre-stamped with compound)
323
144628010 vl either simultaneously with 2 x Ub-Rhl lO or preincubated with USP28 40 minutes prior to the addition of 10 μΐ, of 2 x Ub-Rhl lO to compound plates. Plates were incubated stacked for 90 minutes at room temperature before fluorescence was read on the Envision (Excitation at 485 nm and Emission at 535 nm; Perkin Elmer) or on the PheraSTAR (Excitation at 485 nm and Emission at 535 nm; BMG Labtech),
[00650] For follow-up studies, the assay was performed in a final volume of 15 μΕ in assay- buffer containing 20 mM Tris-HCl (pH 8.0, (1M Tris-HCl, pH 8.0 solution; Corning 46-031- CM)), 3mM BME (2-Mercaptoethanol; Sigma 63689-25ML-F), 0,03% BGG (0.22 μΜ filtered, Sigma, G7516-25G), and 0.01% Triton X-100 (Sigma, T9284-10L). Nanoliter quantities of either an 8-point or 10-point, 3-fold serial dilution in DMSO was pre-dispensed into assay plates (Perkin Elmer, ProxiPlate-384 F Plus) for a final test concentration of either 25 μΜ to 11 nM or 25 μΜ to 1.3 nM, respectively. Enzyme USP28, construct USP28 (USP28-5(1-1077)-TEV- 6*His; LifeSensors) concentration and incubation times were optimized for the maximal signal- to-background while maintaining initial velocity conditions at a fixed substrate concentration. The final concentration of the enzyme in the assay was 75 pM. Final substrate (Ub-Rhl lO; Ubiquitin-Rhodamine 110, R&D Systems #U-555) concentration was 25 nM with [Ub- Rhl l0]«Km. 5 μΕ of 2x enzyme was added to assay piates (pre-stamped with compound) preincubated with USP28 for 30 minutes and then 5 μΐ. of 2 x Ub-Rhl l O was added to assay plates. Plates were incubated stacked for 20 min at room temperature before 5 μΕ of stop solution was added (final concentration of 10 mM citric acid (Sigma, 251275-500G)). Fluorescence was read on the Envision (Excitation at 485 nm and Emission at 535 nm; Perkin Elmer) or on the PheraSTAR (Excitation at 485 nm and Emission at 535 nm; BMG Labtech),
Example 118: Biochemical Assay: Ubiquitin-Rhodamine 110 Assay for USP25 Activity,
[00651] The assay was performed in a final volume of 9 μΕ in assay buffer containing 20 mM Tris-HCl (pH 8.0, (1M Tris-HCl, pH 8.0 solution; Corning 46-031-CM)), 3 mM BME (2- Mercaptoethanol; Sigma 63689-25ML-F), 0.03% BGG (0,22 μ filtered, Sigma, G7516-25G), and 0.01% Triton X-100 (Sigma, T9284-10L). Nanoliter quantities of 10-point, 3-fold serial dilution in DMSO was pre-dispensed into 1536 assay plates (Corning, #3724BC) for a final test concentration of 25 μΜ to 1.3 nM, top to lowest dose, respectively. Enzyme USP25, construct USP25-His6, (Boston Biochem E-546). Concentration and incubation times were optimized for
324
144628010 vl the maximal signal-to-background while maintaining initial velocity conditions at a fixed substrate concentration. The final concentration of the enzyme in the assay was 75 pM. Final substrate (Ub-Rhl lO; Ubiquitin-Rhodamine 1 10, R&D Systems #U~555) concentration was 25 nM with [Ub-Rhl l0]«Km. 3 \xL of 2x enzyme was added to assay plates (pre-stamped with compound) preincubated with USP25 for 30 minutes and then 3 fiL of 2 x Ub-Rh l 10 was added to assay plates. Plates were incubated for 45 minutes at room temperature before addition of 3 uL of stop solution (final concentration of 10 raM citric acid (Sigma, 251275-500G)). Fluorescence was read on the Envision (Excitation at 485 nm and Emission at 535 nm; Perkin Elmer) or on the PheraSTAR (Excitation at 485 nm and Emission at 535 nm; BMG Labtech),
[00652] For both the USP28 and USP25 assay formats, data were reported as percent inhibition compared with control wells based on the following equation: %inh = 1 -((FLU - Ave ) / (AveHigh - AveLow)) where FLU = measured Fluorescence, Ave w = average Fluorescence of no enzyme control (n=16), and
Figure imgf000326_0001
average Fluorescence of DMSO control (n=16). ICso values were determined by curve fitting of the standard 4 parameter logistic fitting algorithm included in the Activity Base software package; IDBS XE Designer Model205. Data is fitted using the Levenburg Marquardt algorithm.
[00653] Table 28: USP28 and USP25 activities of compounds of the disclosure in USP28 and USP25 assays. ++++ indicates an ICso of less than about 0.2 μΜ, +++ indicates an ICso between about 0.2 μΜ and about 2 μΜ, ++ indicates an ICso between about 2 μΜ and about 10 μΜ, and
+ indicates an ICso between about 10 μΜ and about 25 μΜ. D indicates that the data has not been determined.
TABLE 28: USP28 and USP25 Assays
Figure imgf000326_0002
Figure imgf000326_0003
325
144628010 vl Compound Compound
USP28 ICso USP25 ICso USP28 ICso USP25 ICso
No. No.
145 4-4-4-4- 4-4-4- 1-48 -r -r-r 4-4-4-4-
1-16 4-4-H- 1-49 4.4.4.4 ND
1-17 +++ 4.4. 1-50 4-4-4- ND
1-18 4-4-4-4- ++++ 1-51 -H--H- ND
1-19 4-4-4-4- 4.4.4.4. 1-52 4.4.4.4. 4.4.4.
1-20 . . . ++ . 1-53 4.4.44. 4-4-4-4-
1-21 4-4-4-4- ++++ 1-54 4-4-4-
1-22 4-4-4-4- +++ 1-55 t† † ND
1-23 4..1.4. 4.4. 1-56 4-4-4- 4.4.4.
1-24 4-4- + 1-57 4-4-4- ND
1-25 . . . ++ 1-58 4.4.4.4. 4.4.4.
1-26 -i-h- ++ 1-59 +4-4- 4-4-4-
1-27 + 1-60 4-4-4- .,4..,
1-28 .1.4. 1-61 4-4-4- 4-4-
1-29 4-4- +4- 1-62 -i-4~i-4- 4-4-4-4-
1-30 ++ + 1-63 i~ri~r 4-4-4-
1-31 4-4- 4.4. 1-64 4.4. 4. 4-4-4-4-
1-32 4-4- ND 1-65 4.4.4.4 4-4-4-4-
Ϊ-33 ++ ++ 1-66 -! -T-! -T ND
1-34 4.44.4. ++4. 1-67 4.4..I.4. 4-4-4-4-
1-35 4-4-4-4- ND 1-68 4-4-4- 4-4-
1-36 4-4-4-4- +++ 1-69 -(— f -ί- 4-4-4-
Ϊ-37 4-4-4-4- +++ 1-70 --i— Ϊ— i- 4-4-4-
1-38 +++ 4.4 1-71 4-4-4- .,4..,
1-39 4-4- ND 1-72 .1.4.44. 4..1.4.
1-40 : i : ++ 1-73 4-4-4- 4-4-4-
1-41 ++++ +++ 1-74 i~ri~r ND
1-42 4.4.4. ND 1-75 4-4-4- 4.4.4.
1-43 44.4 4. 1-76 4.4.4..1. 44..,
1-44 ++++ ++++ 1-77 4-4-4- 4-4-4-
1-45 4.4.4.4. ND 1-78 4.4.4.4. 4.4.4.
1-46 44.4 ND 1-79 4-4-4- 44..,
1-47 ++++ ++++ 1-80 4-4- ND 4628010 vl Compound Compound
USP28 ICso USP25 ICso USP28 ICso USP25 ICso
No. No.
1-81 ++++ ++++ 1-114 -r -r-r
I~82 ++++ 1-115
1-83 +++ +++ 1-116 +++
1-84 +++ 1-117 +++ ND
1-85 +++ 1-118 +++ ND
1-86 +++. 1-119 ND
1-87 ++++ +++ 1-120 ND
1-88 ++++ +++ 1-121 +++ +-+-+
1-89 +++. 1-122 +++
1-90 +++ 1-123 +-[-++
1-91 +++ 1-124 +++ ++
1-92 -i-h- +++ 1-125 +++ ND
1-93 1-126 +++ ++
1-94 +++ +++ 1-127 +++ +++
1-95 ++++ +++ 1-128 +++ ++
1-96 ++++ +++ 1-129 +++ ND
1-97 +-}-+ -}-+ 1-130
1-98 +++ 1-131
Ϊ-99 ++ ++ 1-132 ++++
1-100 +++ 1-133 ND
I- 101 ++ 1-134 + -[- + +
1-102 ++ ++ 1-135 ++++ +++
1-103 i~H- +++ 1-136 +++
1-104 +++ 1-137 + ++ ND
1-105 +++ +++ 1-138 +++
1-106 -t~M- ++ 1-139 +++ ND
1-107 + 1-140
1-108 +++. 1-141 +++
1-109 1-142
mo ++ 1-143 ND
I-lll ++++ +++. 1-144 +++
1-112 +++ 1-145 +++
1-113 ++++ D 1-146 +++4628010 vl Compound Compound
USP28 ICso USP25 ICso USP28 ICso USP25 ICso
No. No.
1-147 ++++ ND 1-180 -r -r-r +4-+
1-148 +++ 4.4 1-181 4-1-44 .,4..,
1-149 +++ +++ 1-182 4.4.4. ++
1-150 44-4- ++4- 1-183 +4-+ ++
1-151 44-4- +++ 1-184 44.4 4.
1-152 ++ 1-185 4.4 ND
1-153 †t† ++ 1-186 + - 4-
1-154 ++ 44- 1-187 +++ ND
1-155 4.4.4. 4.++ 1~ ί 88 4-4-1-4- 4.44.
1-156 44.4 4.4 1-189 +++ ++
1-157 + 1-190 44.4 4.
Ϊ-158 ++ ++ 1-191 +++ ++
1-159 .,4.4 4. 1492 44.4. ++
1-160 4.4. .1-4 1493 -1-4-1-4- 4.44. ϊ-161 44-4- ++ 1-194 4444- +++
1-162 ! 1 ! +++ 1-195 i~ri~r +++
1-163 4.4.4. -}-4- 1-196 4.4.4. 4.4.4.
1-164 4.4 4 1-197 -j-4 4.
1-165 T-!-T + 1-198 ++ 4-
1-166 ++ + 1-199 4.4.4. 4.44.
1-167 ++ 4-1- 1-200 44.4. 44..,
1-168 ++ + 1-201 +++ +++
1-169 ++++ +4+ Ϊ-202 +++ ND
1-170 4.44.4. 4.44. 1-203 44.4.
1-171 4.44. 4.4.4. 1-204 4.4.4. 4..H.+
1-172 ++++ ++++ 1-205 -†4-† +4-+
1-173 4-4-4- +++ 1-206 i~ri~r ++++
1-174 4.44. -t-4- 1-207 4.4.4. ND
1-175 4.4. 4.-1- 1-208 44.4 4.4.
1-176 4-4-4- +++ 1-209 4- ND
1-177 +4-4- 4.4.4. 1-210 4.4.4. ++
1-178 4.4 4-1- 1-211 44.4. ++
1-179 ++ ++ 1-212 +++ +++ 4628010 vl Compound j Compound
USP28 ICso USP25 ICso USP28 ICso USP25 ICso
No. No.
1-213 i~H- +++ 1-220 ++ ++
1-214 +++ 4 1-221 + 4.
1-215 444- .4. 1 1-222 +++ 4.4.4.
1-216 +-H- +++ ++ +++
1-217 4-4.4. +++
1-218 4 .4. +-H-
1-219 +++
[00654] Those skilled in the art will recognize, or be able to ascertain, using no more than routine experimentation, numerous equivalents to the specific embodiments described specifically herein. Such equivalents are intended to be encompassed in the scope of the following claims.
329
144628010 vl

Claims

CLAIMED:
Figure imgf000331_0001
or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, and tautomer thereof!, wherein:
X is N or CR-:
Ri is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NRgRio;
R2 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloal koxy,
(Cj-Ce) hydroxyalkyl, halogen, (Cs-Cs) cycloalkvl, or -NRnR12;
R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-C6) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyal kyl , halogen, (C3-Cs) cycloalkyl, or -NR13R14;
wherein at least one of Ri, R2, or R3 is not H; or Ri and R2 together form a (C4-Cg) cycloalkyl optionally substituted with one or more
Rl5, or R2 and R3 together form a (C4-Cs) cycloalkyl optionally substituted with one or more
R15;
R4 is H, (Ci-Ce) alkyl, or (Ci-Ce.) haloalkyl;
R5 is FL (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloalkyl,
R. is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloalkyl; or R.4 and 5 together with the carbon atoms to which they are attached form a (Cs-Cg) cycloalkyl ring;
Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
-O-heterocycloaikyi, -(C0-C3) aikyiene-aryi, -(C0-C3) aikyiene-heteroaryl or
~N(R8)~(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more R16; each R7 is independently at each occurrence H, (Ci-Ce) alk l, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyi, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, (C3-Cg) cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, wherein the alkyi is optionally substituted with one or more (Ci-Ce) alkoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R17; or
R& and R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring optionally substituted with one or more Rig; or Re and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris; R6 and R? together when on adjacent atoms form an aryl ring optionally substituted with one or more Rig; or Re and R? together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more Ris; or two R? together when on adjacent atoms form a (C4-Cg) cycloalkyl ring; or two R? together when on adjacent atoms form a heterocycloalkyl ring; two R? together when on adjacent atoms form an aryl ring; or two R? together when on adjacent atoms form a heteroaryl ring;
Rs is H or (Ci-Ce) alkyl; each R9, Rio, Ri i, R12, R13, and RM is independently H, (Ci-Ce) alkyl, or
-C(Q)(Ci-Ce) alkyl; each Ris is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyi, (Ci-Ce) haloalkoxy, halogen, or -OH; each Rie is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloal kyi , (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyal kyl , halogen, (C3-Cs) cycloalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloaikyi; or two Ri& together when attached to the same carbon can form -C=(0) when R& is
-(Co-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (C3-Cs) spirocycloaikyi optionally substituted with one or more R23 when Re is
-(C0-C3) alkylene-heterocycloal kyl , -O-heterocycloalkyl, or
-N(Rs)-(Co-C3) alkylene-heterocycloalkyl; or two Rie together when attached to the same atom form a (Cs-Cs) spiroheterocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl; or two R10 together when on adjacent atoms form a heterocycloaikyi ring optionally substituted with one or more R23; or two Rie together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more R23; or two R10 together with the atoms to which they are attached can form a bridged heterocycloai kyi ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or -N(Rs)-(Co-C3) alkylene-heterocycloal kyl ,
each Ri7 is independently at each occurrence (Ci-C6) alkyl, (Ci-C6) alkoxy,
(Ci-Ce) haloal kyl , (Ci-Ce) haloalkoxy, halogen, cycloalkyl, heterocycloai kyi , or
-C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy and -OH; each Rig is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-C6) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, -C(0)OH, or -C(0)0(Ci-Ce) alkyl; each Ri9 and R20 is independently H, (Ci-Ce) alkyl, (C3-C8) cycloalkyl, -CH2C(0)NH2, -S(0)2(Ci-C6) alkyl, -S(0)2(C6-Cio) aryl or -C(0)(Ci-Ce) al kyl , each R21 and R22 is independently H or (Ci-Ce) alkyl; each R2.3 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, or halogen, or two R23 together when attached to the same carbon form -C=(0); m is 1 or 2; and n is 0, 1, 2, or 3.
Figure imgf000334_0001
or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, and tautomer thereof!, wherein:
X is N or CR?;
Ri is H, (Ci-Ce) alkyl, (C -Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, -CN, or -NR9R10;
R2 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-C») cycloalkyl, or -NRnR-i;
R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, (Ci-C6) haloalkyl, (Ci-Ce) haloalkoxy,
(Ci-Ce) hydroxyalkyl, halogen, (C3-Cs) cycloalkyl, or --NR13R14;
wherein at least one of Ri, R2, or R3 is not H; or Ri and R2 together form a (C4-Cs) cycloalkyl optionally substituted with one or more
Rl5, or R2 and R3 together form a (C4-Cs) cycloalkyl optionally substituted with one or more
R15;
R4 is H, (Ci-Ce) alkyl, or (Ci-Ce) haloalkyl;
R5 is FL (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloalkyl, Rs' is H, (Ci-Ce) alkyl, halogen, or (Ci-Ce) haloalkyl;
Re is -(C0-C3) alkylene-C(0)OH, -(C0-C3) alkylene-heterocycloalkyl,
-0-heterocycloalkyl,-(Co-C3) alkylene-aryl, -(C0-C3) alkylene-heteroaryl or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl, wherein the heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more Rie; each R7 is independently at each occurrence H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, (Cs-Cs) cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, wherein the alkyl is optionally substituted with one or more (Ci-Ce) alkoxy or -OH, and wherein the cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more R17; or
Re and R? together when on adjacent atoms form a (C4-Cs) cycloalkyl ring optionally substituted with one or more Rig; or R6 and R? together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more Ris; Re and R? together when on adjacent atoms form an aryl ring optionally substituted with one or more Ris; or Re and R? together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more Ris; or two R? together when on adjacent atoms form a (C4-Cx) cycloalkyl ring; or two R? together when on adjacent atoms form a heterocycloalkyl ring; two R? together when on adjacent atoms form an aryl ring; or two R? together when on adjacent atoms form a heteroaryl ring;
Rs is H or (Ci-Ce) alkyl; each R9, Rio, Rii, R12, R13, and R14 is independently H, (Ci-Ce) alkyl, or
-C(0)(Ci-Ce) alkyl; each Ris is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloal koxy, halogen, or -OH; each R16 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, (Ci-Ce) hydroxyalkyl, halogen, (Cs-Cs) cycloalkyl, -C(0) 2jR22, -S(0)2(Ci-Ce) alkyl, -OH, or -NR19R20, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy, OH, and heterocycloalkyl; or two Ri& together when attached to the same carbon can form -C=(0) when R& is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycloalkyl, or -N(Rs)-(Co-C3) alkylene-heterocycloalkyl; or two Ri6 together when attached to the same atom form a (C3-Cs) spirocycloalkyl optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O- heterocycloalkyl, or -N(Rs)-(C0-C3) alkylene-heterocycloal kyl ; or two Ri6 together when attached to the same atom form a (C3-Cs) spiroheterocycloalkyl optionally substituted with one or more R23 when Re. is -(C0-C3) al kylene-heterocycloalkyl, -O-heterocycloalkyl, or
-N(R8)-(Co-C3) alkylene-heterocycloalkyl; or two R10 together when on adjacent atoms form a heterocycloalkyl ring optionally substituted with one or more R23; or two Rie together when on adjacent atoms form a heteroaryl ring optionally substituted with one or more R2.3; or two Ri6 together with the atoms to which they are attached can form a bridged heterocycloalkyl ring optionally substituted with one or more R23 when Re is -(C0-C3) alkylene-heterocycloalkyl, -O-heterocycioalkyl, or -N(Rs)-(Co-C3) alkylene-heterocycloalkyl; each Ri7 is independently at each occurrence (C1-C0) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, cycloalkyl, heterocycloalkyl, or
-C(0)-heterocycloalkyl, wherein the alkyl is optionally substituted with one or more substituents independently selected from (Ci-Ce) alkoxy and -OH; each Ri8 is independently at each occurrence (C i -( V.) alkyl, {(' ; -< V.) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, halogen, -OH, -CN, -C(Q)OH, or -C(0)0(Ci-Ce) alkyl; each Ri9 and R20 is independently H, (Ci-Ce) alkyl, ·; (\-{ 'κ) cycloalkyl, -CH2C(0) H2, •S{()) H( : ·( ',.} alkyl, -S(0)2(Ce-Cio) aryl or C(O){0 i-(V) alkyl; each R?J and R22 is independently H or (Cj-Ce) alkyl; each R23 is independently at each occurrence (Ci-Ce) alkyl, (Ci-Ce) alkoxy,
(Ci-Ce) haloalkyl, (Ci-Ce) haloalkoxy, or halogen; or two R23 together when attached to the same carbon form -C=(0); and n is 0, 1, 2, or 3.
3. The compound of claim 1 or 2, wherein X is CH.
4. The compound of any one of claims 1 to 3, wherein R4 is H or CH3.
5. The compound of any one of claims 1 to 4, wherein R5 is H or CH3.
6. The compound of any one of claims 1 to 5, wherein R.v is H.
7. The compound of any one of claims 1 to 6, wherein R2 is H, (Ci-Ce) alkyl,
(C1-C0) haioalkyl, halogen, (C3-Cs) cycloaikyi, or -NEb.
8. The compound of any one of claims 1 to 6, wherein Ri and R2 together form a
(C3-C8) cycloaikyi optionally substituted with one or more R15.
9. The compound of any one of claims 1 to 8, wherein R3 is H, (Ci-Ce) alkyl,
(C1-C&) alkoxy, or (Ci-Ce) haioalkyl.
10. The compound of any one of claims 1 to 6, wherein R2 and R3 together form a (C3-C8) cycloaikyi optionally substituted with one or more R15.
11. The compound of any one of claims 1 to 10, wherein n is 0.
12. The compound of any one of claims 1 to 10, wherein n is 1 or 2.
13. The compound of any one of claims 1 to 10, wherein n is 3.
14. The compound of claim 1 having Formula (lb), Formula (Ic), or Formula (Id):
Figure imgf000338_0001
or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, and tautomer thereof.
15. The compound of claim 1 having Fonnuia (le), Formula (If), or Formula (Ig);
Figure imgf000338_0002
Figure imgf000339_0001
or a pharmaceutically acceptable salt, hydrate, solvate, prodmg, stereoisomer, and tautomer thereof.
The compound of claim 1 having Formula (Ih), Formula (Ii), Formula (Ij), c
(Ik):
Figure imgf000339_0002
Figure imgf000340_0001
Figure imgf000340_0002
or a pharmaceutical ly acceptable salt, hydrate, solvate, prodrug, stereoisomer, and tautomer thereof.
17. The compound of any one of claims 14 to 16, wherein R4 is H or CH3.
18. The compound of any one of claims 14 to 17, wherein R5 is H or CH3.
19. The compound of any one of claims 14 to 18, wherein R.v is H.
20. The compound of any one of claims 14 to 19, wherein R2 is H, (Ci-Ce) alkyl,
(Ci-Ce) haloalkyl, halogen, (Cs-Cs) cycloalkyl, or -Nit.
21. The compound of any one of claims 14 to 19, wherein Ri and R2 together form a (C3- Cs) cycloalkyl optionally substituted with one or more R15.
22. The compound of any one of 14 to 21, wherein R3 is H, (Ci-Ce) alkyl, (Ci-Ce) alkoxy, or (Ci-Ce) haloalkyl.
23. The compound of any one of claims 14 to 19, wherein R2 and R3 together form a (C3- Cs) cycloalkyl optionally substituted with one or more R15.
24. The compound of any one of claims 14 to 23, wherein n is 0.
25. The compound of any one of claims 14 to 23, wherein n is 1 or 3.
26. The compound of any one of claims 14 to 23, wherein n is 3.
27. The compound of claim 1, selected from:
Figure imgf000341_0001
Figure imgf000341_0002
Figure imgf000342_0001
Figure imgf000342_0002
Figure imgf000342_0003
Figure imgf000342_0004
Figure imgf000343_0001
Figure imgf000343_0002
i~22, H^i &*i&^&^<}&3>.2 ΐκ ζίί
Figure imgf000343_0003
Figure imgf000343_0004
Figure imgf000344_0001
Figure imgf000344_0002
Figure imgf000344_0003
Figure imgf000344_0004
Figure imgf000345_0002
Figure imgf000345_0003
Figure imgf000345_0004
Figure imgf000346_0001
Figure imgf000346_0002
Figure imgf000346_0003
Figure imgf000346_0004
Figure imgf000347_0001
Figure imgf000347_0002
Figure imgf000347_0003
-75.2^^s--?-i ^-¾¾s&aSy;p5f*!-£-
•74.3^?^ίΓ:Ξ-§^Τ^ί:^^^^^
Figure imgf000347_0004
Figure imgf000348_0001
Figure imgf000348_0002
Figure imgf000348_0003
-85.■:: K-i-ssm<;s<- ¾i-j# s¾s«-o-*4'4 .!Sset
3? . :i¾:-3^i;-^ : ^i
Figure imgf000348_0004
Figure imgf000348_0005
Figure imgf000349_0001
i -32. ;-S:Y;::-:?..:i.n-i:; v;- -;{ ?. X-l- i-
Figure imgf000349_0002
Figure imgf000349_0003
Figure imgf000349_0004
Figure imgf000349_0005
Figure imgf000349_0006
Figure imgf000350_0001
Figure imgf000350_0002
Figure imgf000350_0003
Figure imgf000350_0004
Figure imgf000351_0001
Figure imgf000351_0002
Figure imgf000351_0003
Figure imgf000351_0004
Figure imgf000352_0001
Figure imgf000352_0002
Figure imgf000352_0003
Figure imgf000353_0001
Figure imgf000353_0002
Figure imgf000353_0003
2. !>& ^^2- ?&S%£m«!£e
Figure imgf000353_0004
Figure imgf000354_0001
Figure imgf000354_0002
Figure imgf000354_0003
Figure imgf000354_0004
■sta ps
Figure imgf000354_0005
Figure imgf000355_0001
-
Figure imgf000355_0002
Figure imgf000355_0003
Figure imgf000355_0004
Figure imgf000356_0001
Figure imgf000356_0002
Figure imgf000356_0003
Figure imgf000356_0004
Figure imgf000357_0001
Figure imgf000357_0002
Figure imgf000357_0003
Figure imgf000358_0001
Figure imgf000358_0002
Figure imgf000358_0003
Figure imgf000358_0004
Figure imgf000359_0001
Figure imgf000359_0002
Figure imgf000359_0003
* f , ^ ^y^ii^S^a^^:^^^
i
Figure imgf000359_0004
Figure imgf000360_0001
and
or a pharmaceutically acceptable salt, hydrate, solvate, prodrug, stereoisomer, and tautomer thereof.
28. A pharmaceutical composition comprising, a compound of any one of claims 1 to 27, and a pharmaceutically acceptable carrier,
29. A method of treating a disease or disorder associated with inhibition of USP28 comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims J to 27.
30. The method of claim 29, wherein the disease or disorder associated with inhibition of USP28 is cancer,
31. A method of inhibiting USP28 comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
32. A method of treating cancer, the method comprising administering, to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
33. The method of claim 32, wherein the cancer is acute myeloid leukemia, gastric cancer, pancreatic cancer, colorectal cancer, glioblastoma, neuroblastoma, small-cell lung cancer, non-small cell lung cancer, or squamous cell carcinoma.
34. The method of claim 32, wherein administration of the compound induces a change in the cell cycle, cell viability, cell apoptosis, or differentiation.
35. A method of treating a disease or disorder associated with inhibition of USP25 comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
36. A method of treating a disease or disorder associated with inhibition of USP28 and USP25 comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
37. The method of claims any one of 29 to 36, wherein administering is performed orally, parentally, subcutaneously, by injection, or by infusion,
38. A compound of any one of claims J to 27 for use in the manufacture of a medicament for treating a disease associated with inhibiting USP28.
39. Use of a compound of any one of claims 1 to 27 in the treatment of a disease associated with inhibiting USP28.
40. A compound of any one of claims 1 to 27 for use in the manufacture of a medicament for treating a disease associated with inhibiting USP25.
41. Use of a compound of any one of claims 1 to 27 in the treatment of a disease associated with inhibiting USP25.
42. A compound of any one of claims 1 to 27 for use in the manufacture of a medicament for treating a disease associated with inhibiting USP28 and USP 25. Use of a compound of any one of claims 1 to 27 in the treatment of a disease associated with inhibiting USP28 and USP 25.
A method of treating inflammation, the method comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
A method of treating an autoimmune disease, the method comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to ? 7
A method of treating an infectious disease, the method comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
The method of claim 46, wherein the infectious disease is a viral infection or a bacterial infection.
A method of treating a viral infection, the method comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims I to 27.
A method of treating bacterial infection, the method comprising, administering to a patient in need thereof an effective amount of a compound of any one of claims 1 to 27.
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020033709A1 (en) * 2018-08-09 2020-02-13 Forma Therapeutics, Inc. Inhibiting deubiquitinase usp25 and usp28
JP2020530469A (en) * 2017-08-11 2020-10-22 フォーマ セラピューティクス，インコーポレイテッド Carboxamide as a ubiquitin-specific protease inhibitor
WO2021105960A1 (en) 2019-11-29 2021-06-03 Lupin Limited Substituted tricyclic compounds
WO2022035806A1 (en) * 2020-08-10 2022-02-17 Dana-Farber Cancer Institute, Inc. Fused tricyclic pyrimidine-thieno-pyridine small molecule inhibitors of ubiquitin-specific protease 28
JP2022532195A (en) * 2019-05-09 2022-07-13 杭州普済遠成生物医薬科技有限公司 Ubiquitin-specific protease inhibitors and their production methods and applications
WO2022157629A1 (en) 2021-01-19 2022-07-28 Lupin Limited Pharmaceutical combinations of sos1 inhibitors for treating and/or preventing cancer
WO2023107722A1 (en) * 2021-12-10 2023-06-15 Prothena Biosciences Limited Compounds for use in treating neurological disorders

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112898314A (en) * 2020-11-06 2021-06-04 刘丽萍 Preparation and application of deubiquitinase inhibitor
CN113499431A (en) * 2021-07-18 2021-10-15 南昌大学第二附属医院 Application of deubiquitinating enzyme USP28 in preparation of medicine for preventing or treating pancreatic cancer
CN115246842B (en) * 2022-06-15 2024-05-24 深圳湾实验室 Small molecule inhibitors targeting deubiquitinase USP25 and USP28

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5262564A (en) 1992-10-30 1993-11-16 Octamer, Inc. Sulfinic acid adducts of organo nitroso compounds useful as retroviral inactivating agents anti-retroviral agents and anti-tumor agents
WO2005037845A1 (en) * 2003-10-17 2005-04-28 Rigel Pharmaceuticals, Inc. Benzothiazole and thiazole[5,5-b] pyridine compositions and their use as ubiquitin ligase inhibitors
WO2006068618A1 (en) * 2004-12-23 2006-06-29 Astrazeneca Ab New compounds
WO2010099166A1 (en) * 2009-02-27 2010-09-02 Siga Technologies, Inc. Thienopyridine derivatives for the treatment and prevention of dengue virus infections
WO2012040527A2 (en) * 2010-09-24 2012-03-29 The Regents Of The University Of Michigan Deubiquitinase inhibitors and methods for use of the same
WO2014105952A2 (en) * 2012-12-28 2014-07-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Inhibitors of the usp1/uaf1 deubiquitinase complex and uses thereof
WO2014116859A1 (en) * 2013-01-23 2014-07-31 The University Of Chicago Methods and compositions for inhibiting human copper trafficking proteins atox1 and ccs

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5550800A (en) 1999-06-22 2001-01-09 School Of Pharmacy, University Of London, The Diagnosis and treatment of alzheimer's disease
AR075402A1 (en) 2009-02-13 2011-03-30 Sanofi Aventis OXYGENATE AND / OR NITROGEN HETEROCICLIC DERIVATIVES OF TETRAHYDRONAFTALENE, DRUGS THAT CONTAIN THEM AND USE OF THE SAME IN THE TREATMENT OF METABOLIC DISORDERS, SUCH AS OBESITY, AMONG OTHERS.

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5262564A (en) 1992-10-30 1993-11-16 Octamer, Inc. Sulfinic acid adducts of organo nitroso compounds useful as retroviral inactivating agents anti-retroviral agents and anti-tumor agents
WO2005037845A1 (en) * 2003-10-17 2005-04-28 Rigel Pharmaceuticals, Inc. Benzothiazole and thiazole[5,5-b] pyridine compositions and their use as ubiquitin ligase inhibitors
WO2006068618A1 (en) * 2004-12-23 2006-06-29 Astrazeneca Ab New compounds
WO2010099166A1 (en) * 2009-02-27 2010-09-02 Siga Technologies, Inc. Thienopyridine derivatives for the treatment and prevention of dengue virus infections
WO2012040527A2 (en) * 2010-09-24 2012-03-29 The Regents Of The University Of Michigan Deubiquitinase inhibitors and methods for use of the same
WO2014105952A2 (en) * 2012-12-28 2014-07-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Inhibitors of the usp1/uaf1 deubiquitinase complex and uses thereof
WO2014116859A1 (en) * 2013-01-23 2014-07-31 The University Of Chicago Methods and compositions for inhibiting human copper trafficking proteins atox1 and ccs

Non-Patent Citations (42)

* Cited by examiner, † Cited by third party
Title
BEROUKHIM ET AL., NATURE, vol. 463, 2010, pages 899 - 905
BRADLEY; POBER, ONCOGENE, vol. 20, 2001, pages 6482 - 6491
CONACCI-SORRELL ET AL., COLD SPRING HARB. PERSPECT. MED., vol. 4, 2014, pages 1 - 24
CUI ET AL., HUM VACCIN. IMMUNOTHER, vol. 10, 2014, pages 3270 - 3285
DIEFENBACHER ET AL., J. OF ('IIN. INVESTI., vol. 1-14, 2014, pages 3407 - 3418
E. L. ELIEL; S. H WILEN; L. N MANDER: "Stereochemistry of Organic Compounds", 1994, WILEY-INTERSCIENCE
FLUGEL ET AL., BLOOD, vol. 119, 2012, pages 1292 - 1301
FRANCIS ET AL., JOURNAL OF MEDICINAL CHEMISTRY, vol. 34, no. 8, 1991, pages 2570 - 2579
GABAY ET AL., COLD SPRING HARB. PERSPECT. MED., 2014
GUO ET AL., TUMOR BIO., vol. 35, 2014, pages 4017 - 4022
HUANG ET AL., COLD SPRING HARB. PERSPECT. MED., 2013
IWAKURA ET AL., IMMUNITY, vol. 34, 2011, pages 149 - 162
KNOBEL ET AL., MOLECULAR AND CELLULAR BIOLOGY, vol. 34, 2014, pages 2062 - 2074
KOMANDER ET AL., ALOL. CELL BIO., vol. 10, 2009, pages 550 - 563
LI ET AL., ALOL. CELL, vol. 13, 2014, pages 166 - 170
MENG ET AL., CANCER RES., vol. 69, 2009, pages 573 - 582
METZGER ET AL., NATURE, vol. 437, 2005, pages 436 - 439
MEYER ET AL., NAT. REV. CANCER, vol. 8, 2008, pages 976 - 990
MOLANDER, G. A. ET AL., ./. ORG. CHEM., vol. 72, 2007, pages 8422
NIJMAN ET AL., CELL, vol. 123, 2005, pages 773 - 786
PERIZ ET AL., PLOS BIOLOGY, 2015
POPOV ET AL., NAT. CELL. BIOL., vol. 9, 2007, pages 729 - 731
POPOV, NAT. CELL BIOL., pages 765 - 774
POPOV, NAT. CELL. BIOL., pages 765 - 774
ROUSSEL ET AL., COLD SPRING HARB. PERSPECT. MED., 2013
SANKAR ET AL., CLIN CANCER RES., 2014, pages 4584 - 4597
SCHENK ET AL., NAT. MED., vol. 18, 2012, pages 605 - 611
SCHMITZ ET AL., COLD SPRING HARB. PERSPECT. MED., 2014
STOECK ET AL., CANCER DISCOV., vol. 4, 2014, pages 1154 - 1167
T. W. GREENE; P. G. M. WUTS: "Protective Groups in Organic Synthesis", 1999, WILEY
TOFFOLO ET AL., NEUROCHEM., vol. 128, 2014, pages 603 - 616
UYEN LE ET AL: "Discovery of a selective M4 positive allosteric modulator based on the 3-amino-thieno[2,3-b]pyridine-2-carboxamide scaffold: Development of ML253, a potent and brain penetrant compound that is active in a preclinical model of schizophrenia", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 23, no. 1, 1 January 2013 (2013-01-01), pages 346 - 350, XP055081743, ISSN: 0960-894X, DOI: 10.1016/j.bmcl.2012.10.073 *
WALSH ET AL., IMMUNOL. REV., vol. 1, 2015, pages 72 - 92
WANG ET AL., EXP. BIOL. MED., 2016, pages 255 - 264
WENG ET AL., SCIENCE, vol. 306, 2004, pages 269 - 271
WU ET AL., CELL REP., vol. 5, 2013, pages 224 - 236
WU, CELL REP., pages 224 - 236
ZHANG ET AL., ./. CELL. MOL. MED., vol. 19, 2015, pages 799 - 805
ZHANG ET AL., CELL, vol. 126, 2006, pages 529 - 542
ZHONG ET AL., NAT. IMMUNOL., vol. 13, 2012, pages 1110 - 1117
ZHONG ET AL., PLOS ONE, vol. 8, 2013, pages E80976
ZHONG ET AL., SCI. SIGNAL., vol. 6, 2013, pages RA35

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* Cited by examiner, † Cited by third party
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US11524966B1 (en) 2017-08-11 2022-12-13 Valo Health, Inc. Carboxamides as ubiquitin-specific protease inhibitors
CN112823037A (en) * 2018-08-09 2021-05-18 瓦洛早期发现股份有限公司 Inhibition of deubiquitinase USP25 and USP28
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JP7398135B2 (en) 2019-05-09 2023-12-14 杭州普済遠成生物医薬科技有限公司 Ubiquitin-specific protease inhibitor and its production method and application
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EP3954694A4 (en) * 2019-05-09 2022-08-24 Chaser Therapeutics, Inc. Ubiquitin-specific protease inhibitors, and preparation method therefor and application thereof
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WO2021105960A1 (en) 2019-11-29 2021-06-03 Lupin Limited Substituted tricyclic compounds
WO2022035806A1 (en) * 2020-08-10 2022-02-17 Dana-Farber Cancer Institute, Inc. Fused tricyclic pyrimidine-thieno-pyridine small molecule inhibitors of ubiquitin-specific protease 28
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