WO2017119739A1 - Cell printing apparatus for heat-sensitive cell printing composition - Google Patents

Cell printing apparatus for heat-sensitive cell printing composition Download PDF

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Publication number
WO2017119739A1
WO2017119739A1 PCT/KR2017/000140 KR2017000140W WO2017119739A1 WO 2017119739 A1 WO2017119739 A1 WO 2017119739A1 KR 2017000140 W KR2017000140 W KR 2017000140W WO 2017119739 A1 WO2017119739 A1 WO 2017119739A1
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WO
WIPO (PCT)
Prior art keywords
cell printing
printing composition
nozzle
heat
heater unit
Prior art date
Application number
PCT/KR2017/000140
Other languages
French (fr)
Korean (ko)
Inventor
안근선
심진형
민경현
윤원수
진송완
Original Assignee
주식회사 티앤알바이오팹
한국산업기술대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to KR20160002008 priority Critical
Priority to KR10-2016-0002008 priority
Priority to KR1020160171709A priority patent/KR101795559B1/en
Priority to KR10-2016-0171709 priority
Application filed by 주식회사 티앤알바이오팹, 한국산업기술대학교산학협력단 filed Critical 주식회사 티앤알바이오팹
Priority claimed from EP17736111.0A external-priority patent/EP3401398A4/en
Publication of WO2017119739A1 publication Critical patent/WO2017119739A1/en

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B33ADDITIVE MANUFACTURING TECHNOLOGY
    • B33YADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y30/00Apparatus for additive manufacturing; Details thereof or accessories therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof

Abstract

A cell printing apparatus according to the present invention comprises: a nozzle through which a heat-sensitive cell printing composition is discharged; and a heating unit provided so as to transmit heat from above the heat-sensitive cell printing composition which is discharged from the nozzle and layered. A set space is provided between the heating unit and nozzle so that the heating unit and nozzle do not come into contact with each other.

Description

Cell printing device of heat sensitive cell printing composition

The present invention relates to a cell printing apparatus, and more particularly, to a cell printing apparatus equipped with a heating module to facilitate lamination of a three-dimensional structure made of a heat sensitive cell printing composition discharged from a nozzle of a cell printing apparatus. It is about.

There is a continuing research on printing cells using 3D printing technology to implement living tissue. This is called "cell printing" and the cell printing composition (10) in the form of gel (2) including collagen, gelatin, alginate, dECM containing cells 1 is discharged through a nozzle of a 3D printer. It is printed. Collagen, gelatin, alginate, dECM, etc. are sometimes referred to as "bioinks".

As used herein, the term "cell printing composition" refers to a liquid substance discharged for 3D cell printing as a liquid substance such as collagen, gelatin, alginate, dECM, or the like in which cells are contained.

2 is a conceptual diagram of a state of printing with a cell printing apparatus according to the prior art.

The cell printing composition 10 stored in the accommodation part 100 is discharged to the outside through the nozzle unit 150. The nozzle unit 150 is supported by the head unit 200 and is movable in three dimensions (A direction, B direction, and C direction) by a driving device (not shown).

The cell printing composition maintains a liquid state at a lower temperature than the first temperature, but has a property of curing at a temperature higher than a predetermined second temperature higher than the first temperature.

When it is discharged through the nozzle unit 150 and laminated on the floor 300 as shown in FIG. 2, if the temperature is lower than the second temperature, the cell printing composition layer of the discharged layer flows down to the side in the uncured state. Because the structure collapses, there is a problem in that cell printing cannot be performed on a desired three-dimensional structure.

In order to prevent this, by providing a lower side heating part 400 that heats the bottom 300, the cell printing composition may be cured by setting the layer on the bottom 300 to a temperature higher than or equal to a second temperature.

However, as the number of laminated layers increases, the further away from the bottom 300, the heat transfer from the lower side heating part 400 is not smooth, resulting in a problem in that the cell printing composition stacked on the top is not cured properly.

An object of the present invention is to provide a cell printing apparatus capable of immediately curing the cell printing composition irrespective of the height of the layer to be laminated by solving the problems of the prior art.

Cell printing apparatus according to the present invention, the nozzle portion for discharging the heat-sensitive cell printing composition; And a heater part provided to transfer heat from an upper side of the thermally sensitive cell printing composition which is discharged and stacked from the nozzle part.

A predetermined space is provided between the heater part and the nozzle part so that the heater part and the nozzle part do not contact each other.

The heater unit may include a heat generating lamp or a heating wire.

The pyrogenic lamp may irradiate light in the visible and infrared regions.

The heater unit may include a plate-shaped body, and the heating lamp or the heating wire may be provided to the plate-shaped body.

The heater unit may have a plane that is the same as or larger than the structure of the cell printing composition to be stacked.

The cell printing apparatus according to the present invention may further include a receiving part in which the cell printing composition is accommodated, and the receiving part may be disposed opposite to the direction in which the heater part views the cell printing composition to be stacked.

According to another aspect of the present invention, the heater portion may be in a form bent toward the cell printing composition to be stacked toward the outside.

The nozzle unit may be provided to penetrate the central portion of the heater unit.

According to the present invention, it is possible to provide a cell printing apparatus capable of rapidly curing an upper layer of the cell printing composition layer laminated in cell printing so as to accurately print a three-dimensional cell structure.

1 is a view showing a cell printing apparatus according to the present invention.

Figure 2 shows a cell printing apparatus according to another embodiment of the present invention.

3 shows a cell printing apparatus according to the prior art.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to a portion for receiving and discharging the cell printing composition among the components of the cell printing apparatus, and description of other components of the cell printing apparatus that is unnecessary for describing the characteristic portions of the present invention will be omitted. For example, a driving device, a control device, or the like of a cell printing device is not related to the features of the present invention and various conventionally known components can be applied. In addition, the present invention relates to a cell printing apparatus using a heat-sensitive cell printing composition, particularly among cell printing compositions.

1 shows a cell printing apparatus according to the present invention. As described above, components not directly related to the description of the present invention are omitted.

The cell printing composition 10 comprises a cell 1 and a hydrogel 2 (bio ink) such as collagen or dECM. The cell printing composition 10 is accommodated in the accommodating part 100 and is discharged to the outside through the nozzle part 150.

The nozzle part 150 is supported by the head part 200, and the head part 200 is movable by a driving device (not shown). The bottom 300 on which the cell printing composition 10 is stacked may be moved instead of the head 200, and the head 200 may be moved only in one or two dimensions.

The heater unit 250 provided below the head unit 200 may use a heating wire, a heat generating lamp 260 such as an infrared lamp, or a heating wire as a component that generates heat downward. The heater unit 250 is preferably provided in the form of a plate, the heat generating lamp 260 of the heater unit 250 preferably irradiates light in the visible and / or infrared region. More specifically, the heater unit 250 is a plate-shaped body and a heat generating lamp 260 or a heating wire is provided to the plate-shaped body, so that the heat can be removed from the upper side of the cell printing composition to be laminated.

A pyrogenic lamp that allows the layer of the cell printing composition (1-1, 1-2, 1-3, etc.) to reach the gelling temperature (second temperature) or higher, especially during the 3D cell printing process. For example, any heat source may be used regardless of its kind as long as it is a lamp or a heat ray that can apply heat to irradiate light in the visible and / or infrared region. Although three cell printing composition layers (1-1, 1-2, 1-3) are shown in FIG. 1, these are merely illustrative and should be interpreted as non-limiting.

Although the bottom 300 is shown to be provided with a bottom side heating unit 400, it should be understood that the bottom side heating unit 400 is not an essential component of the present invention. The lower side heating part 400 may be a plate member that becomes a heat source in a dictionary sense, or may be any other component that transmits heat to the bottom 300.

The heater unit 250 is preferably disposed at the end side of the nozzle unit 150. This is for the better heat transfer to the top layer (1-3) to be laminated. However, the heater unit 250 may be provided to the head unit 200 instead of the nozzle unit 150 as long as the cell printing composition can be discharged and transfer heat on the stacked layer, or the nozzle unit 150 or It may be provided to a component other than the head 200, or may be arranged as a separate component at all.

The heater unit 250 is preferably provided so as not to contact the nozzle unit 150. As shown in FIG. 1, a predetermined space 255 is provided between the nozzle unit 150 and the heater unit 250. This is to prevent the cell printing composition discharged through the nozzle unit 150 from being hardened by rising above the second temperature by the heat of the heater unit 250 in the process of being discharged.

On the other hand, the heater 250 may have a plane equal to or larger than the structure of the cell printing composition stacked as shown in FIG. In this structure, there is an effect that the laminated layer can be easily and quickly cured.

The receiving part 100 in which the cell printing composition 10 is accommodated may be provided inside the head part 200. More specifically, the receiving part 100 may be provided in the head part 200. In the inner side of the head portion 200 may be provided. The accommodating part 100 may be disposed outside the head part 200 instead of the inside.

According to such a structure, the cell printing composition 10 accommodated by the heat generation of the heater unit 250 can be prevented from curing.

2 shows a cell printing apparatus according to another embodiment of the present invention. 2 is similar to the embodiment of FIG. 1 except that the heater unit 250 is curved toward the structure of the cell printing composition stacked toward the outside. The nozzle unit 150 may be disposed to discharge the cell printing composition through the central portion of the heater unit 250. As shown in the embodiment of FIG. 1, a predetermined space 255 may be provided between the nozzle unit 150 and the heater unit 250.

According to the embodiment shown in Figure 2, since the heat applied to the structure to be laminated can be concentrated, there is an effect that can proceed the gelation of the cell printing composition faster. Although the heater unit 250 is illustrated in FIG. 2 to cover the entire stacked structure, the heater unit 250 may have a smaller size if the heater unit 250 is bent toward the stacked structure toward the outside, and the heater unit 250 may have a pot lid shape. Although it may be other forms, any form may be sufficient as it bends toward the structure laminated | stacked toward the outer side. A heat generating lamp 260 may be provided inside the curved heater unit 250.

Next, the operation of the cell printing apparatus according to the present invention will be described.

The cell printing composition 10 of the nozzle unit 150 is discharged from the nozzle unit 150 to the bottom 300. The head part 200 discharges the cell printing composition 10 to the bottom 300 while moving in the direction to be laminated by the driving means. The first layer 1-1 lying on the bottom 300 receives heat applied from the heater unit 250 while being arranged to reach a second temperature or more and to harden in a short time. If the bottom 300 has the prior art lower side heating part 400, the first layer 1-1 may be cured by heat transferred from the lower side heating part 400.

The cell printing composition 10 is cured through the same process while laminating the second layer 1-2 and the third layer 1-3 on the first layer 1-1. As more layers are stacked, heat transfer from the lower side heating unit 400 decreases, but according to the present invention, since the heat transfer from the upper side by the heater unit 250 is continuously performed, the cells of the upper lamination unit 1-3 are maintained. The printing composition also has the effect of being able to cure quickly.

According to the embodiment of FIG. 2, heat generated from the heater unit 250 may be applied to a structure that is more concentrated and stacked, thereby curing the cell printing composition more quickly.

While the invention has been described above with reference to the accompanying drawings, the scope of the invention is determined by the claims that follow, and should not be construed as limited to the embodiments and / or drawings described above. And it should be clearly understood that improvements, changes and modifications apparent to those skilled in the art of the invention described in the claims are included in the scope of the present invention.

Claims (8)

  1. A nozzle unit through which the thermally sensitive cell printing composition is discharged;
    And a heater part provided to transfer heat from an upper side of the thermally sensitive cell printing composition which is discharged and stacked from the nozzle part.
    A predetermined space is provided between the heater unit and the nozzle unit such that the heater unit and the nozzle unit do not contact each other.
    Cell printing device.
  2. The method according to claim 1,
    The heater unit includes a heat generating lamp or a heating wire,
    Cell printing device.
  3. The method according to claim 2,
    The pyrogenic lamp is irradiated with light in the visible and infrared region,
    Cell printing device.
  4. The method according to claim 2,
    The heater unit includes a plate-shaped body,
    The heating lamp or the heating wire is provided in the plate-shaped body,
    Cell printing device.
  5. The method according to claim 4,
    The heater unit has the same or larger plane than the structure of the cell printing composition to be laminated,
    Cell printing device.
  6. The method according to claim 1 or 2,
    Further comprising a receiving portion for receiving the cell printing composition,
    The receiving portion is disposed on the opposite side of the direction facing the cell printing composition in which the heater portion is laminated,
    Cell printing device.
  7. The method according to claim 4,
    The heater unit is curved toward the cell printing composition to be stacked toward the outside,
    Cell printing device.
  8. The method according to claim 7,
    The nozzle portion is provided to penetrate the central portion of the heater portion,
    Cell printing device.
PCT/KR2017/000140 2016-01-07 2017-01-05 Cell printing apparatus for heat-sensitive cell printing composition WO2017119739A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR20160002008 2016-01-07
KR10-2016-0002008 2016-01-07
KR1020160171709A KR101795559B1 (en) 2016-01-07 2016-12-15 Cell Printing Device for Heat-sensitive Cell-printing Composition
KR10-2016-0171709 2016-12-15

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17736111.0A EP3401398A4 (en) 2016-01-07 2017-01-05 Cell printing apparatus for heat-sensitive cell printing composition
US16/029,016 US20180312796A1 (en) 2016-01-07 2018-07-06 Cell printing apparatus for heat-sensitive cell printing composition

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US16/029,016 Continuation US20180312796A1 (en) 2016-01-07 2018-07-06 Cell printing apparatus for heat-sensitive cell printing composition

Publications (1)

Publication Number Publication Date
WO2017119739A1 true WO2017119739A1 (en) 2017-07-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/000140 WO2017119739A1 (en) 2016-01-07 2017-01-05 Cell printing apparatus for heat-sensitive cell printing composition

Country Status (1)

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WO (1) WO2017119739A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130026683A1 (en) * 2011-07-28 2013-01-31 Ng Hou T Liquid inkjettable materials for three-dimensional printing
WO2015066705A1 (en) * 2013-11-04 2015-05-07 University Of Iowa Research Foundation Bioprinter and methods of using same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130026683A1 (en) * 2011-07-28 2013-01-31 Ng Hou T Liquid inkjettable materials for three-dimensional printing
WO2015066705A1 (en) * 2013-11-04 2015-05-07 University Of Iowa Research Foundation Bioprinter and methods of using same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HOFMANN, MANFRED: "3D Printing Gets a Boost and Opportunities with Polymer Materials", ACS MACRO LETTERS, vol. 3, 15 April 2014 (2014-04-15), pages 382 - 386, XP055397724 *
KNOWLTON, STEPHANIE ET AL.: "Bioprinting for Cancer Research", TRENDS IN BIOTECHNOLOGY, vol. 33, no. 9, September 2015 (2015-09-01), pages 504 - 513, XP055397725 *
LANDERS, RUDIGER ET AL.: "Rapid Prototyping of Scaffolds Derived from Thermoreversible Hydrogels and Tailored for Applications in Tissue Engineering", BIOMATERIALS, vol. 23, no. 23, December 2002 (2002-12-01), pages 4437 - 4447, XP004377513 *

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