WO2017100645A2 - Inhibitors of bacterial rna polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols - Google Patents

Inhibitors of bacterial rna polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols Download PDF

Info

Publication number
WO2017100645A2
WO2017100645A2 PCT/US2016/065931 US2016065931W WO2017100645A2 WO 2017100645 A2 WO2017100645 A2 WO 2017100645A2 US 2016065931 W US2016065931 W US 2016065931W WO 2017100645 A2 WO2017100645 A2 WO 2017100645A2
Authority
WO
WIPO (PCT)
Prior art keywords
alkyl
alkenyl
compound
tautomer
salt
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2016/065931
Other languages
French (fr)
Other versions
WO2017100645A3 (en
Inventor
Richard H. Ebright
Joel Freundlich
Nisha MITTAL
Mark JASKOWSKI
Juan SHEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Priority to US15/782,002 priority Critical patent/US10450292B2/en
Publication of WO2017100645A2 publication Critical patent/WO2017100645A2/en
Publication of WO2017100645A3 publication Critical patent/WO2017100645A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/06—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms
    • C07D333/22—Radicals substituted by doubly bound hetero atoms, or by two hetero atoms other than halogen singly bound to the same carbon atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/12—Ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/38—Heterocyclic compounds having sulfur as a ring hetero atom
    • A61K31/381—Heterocyclic compounds having sulfur as a ring hetero atom having five-membered rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
    • C07C49/753—Unsaturated compounds containing a keto groups being part of a ring containing ether groups, groups, groups, or groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/76—Ketones containing a keto group bound to a six-membered aromatic ring
    • C07C49/82—Ketones containing a keto group bound to a six-membered aromatic ring containing hydroxy groups
    • C07C49/83—Ketones containing a keto group bound to a six-membered aromatic ring containing hydroxy groups polycyclic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/76—Ketones containing a keto group bound to a six-membered aromatic ring
    • C07C49/82—Ketones containing a keto group bound to a six-membered aromatic ring containing hydroxy groups
    • C07C49/835—Ketones containing a keto group bound to a six-membered aromatic ring containing hydroxy groups having unsaturation outside an aromatic ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/76—Ketones containing a keto group bound to a six-membered aromatic ring
    • C07C49/84—Ketones containing a keto group bound to a six-membered aromatic ring containing ether groups, groups, groups, or groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/62—Oxygen or sulfur atoms
    • C07D213/63—One oxygen atom
    • C07D213/64—One oxygen atom attached in position 2 or 6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/62—Oxygen or sulfur atoms
    • C07D213/63—One oxygen atom
    • C07D213/64—One oxygen atom attached in position 2 or 6
    • C07D213/643—2-Phenoxypyridines; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/02—Systems containing only non-condensed rings with a three-membered ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated

Definitions

  • New approaches to drug development are necessary to combat the ever-increasing number of antibiotic-resistant pathogens.
  • RNA polymerase is the molecular machine responsible for transcription and is the target, directly or indirectly, of most regulation of gene expression (Ebright, R. (2000) J. Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 1 1, 155-162; Murakami, K. and Darst, S. (2003) Curr. Opin. Structl. Biol. 13, 31-39; Borukhov, S. and Nudler, E. (2003) Curr. Opin. Microbiol. 6, 93-100; Werner, F. (2007) Mol. Microbiol. 65, 1395-1404; Hirata, A. and Murakami, K. (2009) Curr. Opin.
  • Bacterial RNAP core enzyme has a molecular mass of -380,000 Da and consists of one ⁇ ' subunit, one ⁇ subunit, two a subunits, and one ⁇ subunit; bacterial RNAP holoenzyme has a molecular mass of -450,000 Da and consists of bacterial RNAP core enzyme in complex with the transcription initiation factor ⁇ (Ebright, R. (2000) J Mol. Biol.
  • RNAP core subunit sequences are conserved across Gram- positive and Gram-negative bacterial species (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 11, 155-162; Cramer, P. (2002) Curr. Opin. Structl. Biol. 12, 89-97; Murakami and Darst (2003) Curr. Opin. Structl. Biol. 13, 31-39; Borukhov and Nudler (2003) Curr. Opin. Microbiol. 6, 93-100).
  • Bacterial RNAP core subunit sequences are conserved across Gram- positive and Gram-negative bacterial species (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol.
  • RNAP I, RNAP II, and RNAP III contain counterparts of all bacterial RNAP core subunits, but eukaryotic-subunit sequences and bacterial-subunit sequences exhibit only limited conservation (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 11, 155-162; Cramer, P. (2002) Curr. Opin. Structl.
  • Crystal structures have been determined for bacterial RNAP and eukaryotic RNAP II (Zhang et al., (1999) Cell 98, 811-824; Cramer et al., (2000) Science 288, 640-649; Cramer et al., (2001) Science 292, 1863-1876).
  • RNAP complexes with nucleic acids, nucleotides and inhibitors (Campbell, et al. (2001) Cell 104, 901-912; Artsimovitch, et al. (2005) Cell 122, 351-363; Campbell, et al. (2005) EMBOJ. 24, 674-682; Tuske, et al. (2005) Cell 122, 541-522; Temiaov, et al. (2005) Mol.
  • RNAP Bacterial RNAP is a proven target for antibacterial therapy (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Chopra, I. (2007) Curr. Opin. Investig. Drugs 8, 600-607; Villain- Guillot, P., Bastide, L., Gualtieri, M. and Leonetti, J. (2007) Drug Discov. Today 12, 200-208; Mariani, R. and Maffioli, S. (2009) Curr. Med. Chem. 16, 430-454; Ho, M, Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin. Structl. Biol.
  • RNAP subunit sequences are conserved (providing a basis for broad-spectrum activity), and the fact that bacterial RNAP subunit sequences are only weakly conserved in eukaryotic RNAP I, RNAP II, and RNAP III (providing a basis for therapeutic selectivity).
  • the rifamycin antibacterial agents notably rifampin, rifapentine, and rifabutin— function by binding to and inhibiting bacterial RNAP (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Ho, M., Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin. Structl. Biol. 19, 715-723; Floss and Yu (2005) Chem. Rev. 105, 621 -632; Campbell, et al. (2001) Cell 104, 901 - 912; Artsimovitch, et al. (2005) Cell 122, 351-363; Feklistov, A., Mekler, V., Jiang, Q.,
  • the rifamycins bind to a site on bacterial RNAP adjacent to the RNAP active center and prevent the extension of RNA chains beyond a length of 2-3 nt.
  • the rifamycins are first-line treatments for tuberculosis and are the only current first-line treatments for tuberculosis able to kill non-replicating tuberculosis bacteria, to clear infection, and to prevent relapse (Mitchison, D. (2000) Int. J. Tuberc. Lung Dis. 4, 796-806).
  • the rifamycins also are first-line treatments for biofilm-associated infections of catheters and implanted medical devices and are among the very few current antibacterial drugs able to kill non-replicating biofilm-associated bacteria (Obst, G., Gagnon, R.F., Prentis, J. and Richards, G.K. (1988) ASAIO Trans.
  • Resistance to rifamycins typically involves substitution of residues in or immediately adjacent to the rifamycin binding site on bacterial RNAP-- i.e., substitutions that directly decrease binding of rifamycins.
  • a significant and increasing percentage of cases of tuberculosis are resistant to rifampicin (1.4% of new cases, 8.7% of previously treated cases, and 100% of cases designated multidrug-resistant, in 1999-2002; Schluger, N. (2000) Int. J. Tuberc. Lung Dis.
  • the invention provides new compositions of matter—arylpropanoyl phloroglucinols (APPs), arylpropenoyl phloroglucinols (APPUs), and arylcyclopropanecarboxyl phloroglucinols (APPCs)--that inhibit bacterial RNA polymerase and inhibit bacterial growth.
  • APPs arylpropanoyl phloroglucinols
  • APPUs arylpropenoyl phloroglucinols
  • APPCs arylcyclopropanecarboxyl phloroglucinols
  • phloroglucinols with R AP -inhibitory and antibacterial activities by having a different substituted aryl or heteroaryl moiety and/or a different linker between the aryl or heteroaryl moiety and the phloroglucinol-derived moiety.
  • linker between the aryl or heteroaryl moiety and the phloroglucinol-derived moiety can be replaced by a conformationally constrained, olefinic or cyclic linker without loss of RNA-polymerase-inhibitory and antibacterial activities.
  • Certain compounds of this invention exhibit higher RNA-polymerase-inhibitory and antibacterial potencies than previously disclosed arylpropanoyl phloroglucinols.
  • Certain compounds of this invention exhibit higher metabolic stabilities than previously disclosed arylpropanoyl phloroglucinols.
  • Certain compounds of this invention exhibit lower plasma protein binding than previously disclosed arylpropanoyl phloroglucinols.
  • Certain compounds of this invention exhibit potent RNA-polymerase-inhibitory activities (micromolar and submicromolar IC50s) and potent antibacterial activities (micromolar and submicromolar MICs).
  • RNA polymerase functions by binding to the "switch region SW1/SW2 subregion" of RNA polymerase.
  • Applicants have determined crystal structures of complexes containing RNA polymerase and compounds of the invention (Figs. 1-2). Crystals were obtained by soaking 2 (Fig. 1 ; Table 1) or 4 (Fig 2; Table 1) into pre-formed crystals of a complex of Escherichia coli RNA polymerase ⁇ holoenzyme.
  • X-ray diffraction data were collected at a synchrotron light source, structures were solved by molecular replacement, using a previously determined structure of Escherichia coli RNA polymerase ⁇ 70 holoenzyme as the search model, and structures were refined to a resolution of 3.8 A and an R free of 0.29 (2; Fig. 1) and a resolution of 4.0 A and an R f r ee of 0.30 (4; Fig 2).
  • the structures indicate that compounds of the invention bind to the RNA polymerase switch region SW1/SW2 subregion in a position and orientation generally similar to the binding position and binding orientation of previously disclosed arylpropanoyl phloroglucinols.
  • This invention provides crystal structures of a complex containing RNA polymerase and 2 and a complex containing RNA polymerase and 4.
  • RNA polymerase structure and function control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, antibacterial therapy, and drug discovery.
  • the invention provides a compound of the invention which is a compound selected from compounds of structural formulae I)-(VI):
  • R , R , and R each independently is H, (Ci-C 6 )alkyl, (C 2 -C 6 )alkenyl, or
  • (Ci-C 6 )alkoxy which (Ci-C 6 )alkyl, (C 2 -C 6 )alkenyl, or (Ci-C 6 )alkoxy optionally is substituted with halo;
  • R 1 is fluoromethyl, difluoromethyl, fluoroethyl, difluoroethyl, trifluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy, difluoromethoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, alkaryl, alkheteroaryl, aryloxy, heteroaiyloxy, or benzyloxy, which alkaiyl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, tri fluoromethoxy, (Q-C ⁇ alkyl, (C2-C 8 )alkenyl, (Cr
  • R a and R b each independently is H or (C]-C6)alkyl, or R a and R b , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R 3 , R 4 , and R 5 is other than H; or
  • R 1 is (C 2 -C 8 )alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which (C 2 -C8)alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C]-C 8 )alkyl, (C 2 -C 8 )alkenyl, (C 1 -C 8 )alkoxy, aryl, heteroaiyl, aiyloxy, or heteroaryloxy; and R 3 , R 4 , and R 5 each is H;
  • X and Y are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
  • R 2 , R 6 , and R 7 each independently is H, (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, or
  • R 1 is halo, (Ci-Cg)alkyl, (C 2 -C8)alkenyl, (Ci-C 8 )alkoxy, aryl, heteroaryl, alkaiyl, alkheteroaryl, aiyloxy, heteroaryloxy, or benzyloxy, which (Ci-C 8 )alkyl, (C 2 -C8)alkenyl, (Ci-C 8 )alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaiyl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
  • difluoromethyl trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C 2 -C8)alkenyl, (C 1 -C8)alkoxy, aryl, heteroaiyl, aiyloxy, and heteroaryloxy;
  • R a and R b each independently is H or (Ci-C 6 )alkyl, or R a and R b , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino;
  • R 2" , R 6" , and R 7 each independently is H, (C r C 6 )alkyl, (C 2 -C 6 )alkenyl, or (Ci-C 6 )alkoxy, which (Ci-C 6 )alkyl, (C 2 -C 6 )alkenyl, or (Ci-C 6 )alkoxy optionally is substituted with halo;
  • R 1 is fluoro, bromo, iodo, (C2-C 8 )alkyl, (C 2 -C )alkenyl, (C 2 -C 8 )alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which
  • (C2-Cs)alkyl, (C2-C 8 )alkenyl, (C2-Cs)alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaryl, aiyloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Q-C ⁇ alkyl, (C 2 -C 8 )alkenyl, (Ci-C 8 )alkoxy, aryl, heteroaiyl, aryloxy, and heteroaryloxy; and R 3 , R 4 , and R 5 each independently is H, halo, (Ci-C8)alkyl, or (C 2 -C 8 )alkenyl, which (C 1 -C8)alkyl, or (C 2 -C )alkenyl optionally is substitute
  • R a and R b each independently is H or (Ci-C 6 )alkyl, or R a and R b , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R 3 , R 4 , and R 5 is other than H; or
  • R 1 is fluoromethyl, difluoromethyl, trifluromethyl, fluoroethyl, difluoroethyl, trifluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy,
  • difluoromethoxy trifluoromethoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, aryloxy, heteroaryloxy, alkaryl, or alkheteroaryl, which aryloxy, heteroarlyoxy, alkaiyl, or alkheteroaryl optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl. trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-Cg)alkyl, (C 2 -C 8 )alkenyl,
  • X' and Y' are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
  • R , R , and R each independently is H, (C 1 -C 6 )alkyl, (C 2 -C )alkenyl, or (C!-C6)alkoxy, which (Ci-C6)alkyl, (C 2 -C6)alkenyl, or (Ci-C 6 )alkoxy optionally is substituted with halo;
  • R is halo, (Ci-Cg)alkyl, (C 2 -C 8 )alkenyl, (C 1 -Cg)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, atyloxy, heteroaiyloxy, or benzyloxy, which (C 1 -C 8 )alkyl, (C 2 -C 8 )alkenyl, (Ci-C 8 )alkoxy, aryl, heteroaryl, alkaiyl, alklieteroaryl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
  • the invention also provides methods for making a compound of the invention.
  • the invention also provides an assay for inhibition of a RNA polymerase comprising contacting a bacterial RNA polymerase with a compound of the invention.
  • the invention also provides an assay for antibacterial activity comprising contacting a bacterial RNA polymerase with a compound of the invention.
  • the invention also provides a use of a compound of the invention to bind to a bacterial RNA polymerase.
  • the invention also provides a use of a compound of the invention to inhibit a bacterial RNA polymerase.
  • the invention also provides a use of a compound of the invention to inhibit bacterial gene expression.
  • the invention also provides a use of a compound of the invention to inhibit bacterial growth.
  • the invention also provides a use of a compound of the invention to inhibit a bacterial infection.
  • the invention also provides a composition comprising a compound of the invention or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable vehicle.
  • the invention also provides a method for inhibiting the growth of bacteria comprising contacting the bacteria with a compound of the invention or a salt thereof.
  • the invention also provides a method for inhibiting a bacterial RNA polymerase comprising contacting the bacterial RNA polymerase with a compound of the invention or a salt thereof.
  • the invention also provides a method for treating a bacterial infection in a mammal, e.g., a human, comprising administering to the mammal an effective amount of a compound of the invention or a pharmaceutically acceptable salt thereof.
  • the invention also provides a compound of the invention or a pharmaceutically acceptable salt thereof for use in the prophylactic or therapeutic treatment of a bacterial infection.
  • the invention also provides the use of a compound of the invention or a
  • the invention also provides a compound of the invention or a pharmaceutically acceptable salt thereof for use in medical treatment.
  • Fig. 1 shows a crystal structure of a complex containing RNA polymerase and 2 (see Detailed Description of the Invention).
  • 2 is in a stick representation in dark gray.
  • Experimental electron density for 2 is indicated as a gray mesh (F 0 -F c omit map).
  • RNA polymerase backbone atoms in and near 2 binding site are shown in a ribbon representation in light gray.
  • RNA polymerase sidechain atoms that contact 2 are shown in a stick representation in light gray and are labelled with the RNA polymerase subunit ( ⁇ or ⁇ ') and residue number.
  • Fig. 2 shows a crystal structure of a complex containing RNA polymerase and 4 (see Detailed Description of the Invention).
  • 4 is in a stick representation in dark gray.
  • Experimental electron density for 4 is indicated as a gray mesh (F 0 -F c omit map).
  • RNA polymerase backbone atoms in and near 4 binding site are shown in a ribbon representation in light gray.
  • RNA polymerase sidechain atoms that contact 4 are shown in a stick representation in light gray and are labelled with the RNA polymerase subunit ( ⁇ or ⁇ ') and residue number.
  • Halo is fluoro, chloro, bromo, or iodo.
  • Alkyl, alkoxy, alkenyl, alkynyl, etc. denote both straight and branched groups; but reference to an individual radical such as propyl embraces only the straight chain radical, a branched chain isomer such as isopropyl being specifically referred to.
  • Aryl denotes a phenyl radical or an ortho-fused bicyclic carbocyclic radical having about nine to ten ring atoms in which at least one ring is aromatic.
  • Heteroaryl encompasses a radical of a monocyclic aromatic ring containing five or six ring atoms consisting of carbon and one to four heteroatoms each selected from the group consisting of non-peroxide oxygen, sulfur, and N(X) wherein X is absent or is H, O, (C 1 -C 4 )alkyl, phenyl or benzyl, as well as a radical of an ortho-fused bicyclic heterocycle of about eight to ten ring atoms comprising one to four heteroatoms each selected from the group consisting of non-peroxide oxygen, sulfur, and N(X).
  • Alkaryl refers to a group -(Cj-C4)alkyl-aryl.
  • Alkheteroaryl refers to a group -(C 1 -C 4 )alkyl-heteroaryl.
  • Aryloxy refers to a group -O-aryl.
  • Heteroaryloxy refers to a group -O-heteroaryl.
  • binding refers to high-affinity specific binding (i.e., an interaction for which the equilibrium dissociation constant, Kd, is less than about 100 ⁇ and preferably is less than about 10 ⁇ .
  • structures depicted herein are intended to include all stereochemical forms of the structure; i.e., the R and S configurations for each asymmetric center. Therefore, single stereochemical isomers, as well as enantiomeric and diastereomeric mixtures, of the present compounds are within the scope of the invention.
  • structures depicted herein are intended to include compounds which differ only in the presence of one or more isotopically enriched atoms.
  • compounds having the present structures except for the replacement of a hydrogen atom by a deuterium atom or a tritium atom, or except for the replacement of a carbon by a 13 C- or 14 C- enriched carbon atom, are within the scope of this invention.
  • Compounds of this invention may exist in tautomeric forms, such as keto-enol tautomers.
  • the depiction of a single tautomer is understood to represent the compound in all of its tautomeric forms.
  • pharmaceutically acceptable refers to a component that is, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and other mammals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
  • pharmaceutically acceptable salt means any non-toxic salt that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention.
  • Acids commonly employed to form pharmaceutically acceptable salts include inorganic acids such as hydrogen bisulfide, hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid and phosphoric acid, as well as organic acids such as para-toluenesulfonic acid, salicylic acid, tartaric acid, bitartaric acid, ascorbic acid, maleic acid, besylic acid, fumaric acid, gluconic acid, glucuronic acid, formic acid, glutamic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, lactic acid, oxalic acid, para-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid and acetic acid, as well as related inorganic and organic acids.
  • inorganic acids such as hydrogen bisulfide, hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid and phosphoric acid
  • Such pharmaceutically acceptable salts thus include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caprate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-l,4-dioate, hexyne-l,6-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, terephthalate, sulfonate, xylene sulfonate, phenylacetate, phenylprop
  • the pharmaceutically acceptable salt may also be a salt of a compound of the present invention having an acidic functional group, such as a carboxylic acid functional group, and a base.
  • exemplary bases include, but are not limited to, hydroxide of alkali metals including sodium, potassium, and lithium; hydroxides of alkaline earth metals such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, organic amines such as unsubstituted or hydroxyl-substituted mono-, di-, or tri-alkylamines, dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-OH-(Ci-C )-alkylamine), such as N,N-dimethyl-N-(2-hydroxyethyl)amine or tri-(2- hydroxyethyl)amine; N-methyl
  • a salt of a compound of the invention can be useful as an intermediate for isolating or purifying a compound of the invention.
  • administration of a compound of the invention as a pharmaceutically acceptable acid or base salt may be appropriate.
  • pharmaceutically acceptable salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate, citrate, malonate, tartarate, succinate, benzoate, ascorbate, a-ketoglutarate, and a-glycerophosphate.
  • Suitable inorganic salts may also be formed, including hydrochloride, sulfate, nitrate, bicarbonate, and carbonate salts.
  • salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion.
  • a sufficiently basic compound such as an amine
  • a suitable acid affording a physiologically acceptable anion.
  • Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
  • the compound of the invention can be formulated as pharmaceutical compositions and administered to a mammalian host, such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical, or subcutaneous routes.
  • the present compounds may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an
  • compositions and preparations should contain at least 0.1% of active compound.
  • the percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60% of the weight of a given unit dosage form. The amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained.
  • the tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added.
  • a liquid carrier such as a vegetable oil or a polyethylene glycol.
  • any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non-toxic in the amounts employed.
  • the active compound may be incorporated into sustained-release preparations and devices.
  • the active compound may also be administered intravenously or intraperitoneally by infusion or injection.
  • Solutions of the active compound or its salts can be prepared in water, optionally mixed with a nontoxic surfactant.
  • Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of
  • the pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes.
  • the ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage.
  • the liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof.
  • the proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants.
  • the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.
  • Sterile injectable solutions are prepared by incorporating the active compound in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filter sterilization.
  • the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.
  • the present compounds may be applied in pure form, i.e., when they are liquids. However, it will generally be desirable to administer them to the skin as compositions or formulations, in combination with a dermatologically acceptable carrier, which may be a solid or a liquid.
  • Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like.
  • Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants.
  • Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use.
  • the resultant liquid compositions can be applied from absorbent pads, used to impregnate bandages and other dressings, or sprayed onto the affected area using pump-type or aerosol sprayers.
  • Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user.
  • Useful dosages of the compound of the invention can be determined by comparing their in vitro activity, and in vivo activity in animal models.
  • the amount of the compound, or an active salt or derivative thereof, required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.
  • a suitable dose will be in the range of from about 0.5 to about 150 mg/kg, e.g., from about 10 to about 100 mg/kg of body weight per day, such as 3 to about 75 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 120 mg/kg/day, most preferably in the range of 15 to 90 mg/kg/day.
  • the compound is conveniently formulated in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form.
  • the invention provides a composition comprising a compound of the invention formulated in such a unit dosage form.
  • the desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day.
  • the sub-dose itself may be further divided, e.g., into a number of discrete loosely spaced administrations; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye.
  • Compounds identified according to the target and method of this invention would have applications not only in antibacterial therapy, but also in: (a) identification of bacterial RNAP (diagnostics, environmental-monitoring, and sensors applications), (b) labeling of bacterial RNAP (diagnostics, environmental-monitoring, imaging, and sensors applications), (c) immobilization of bacterial RNAP (diagnostics, environmental- monitoring, and sensors applications), (d) purification of bacterial RNA polymerase (biotechnology applications), (e) regulation of bacterial gene expression (biotechnology applications), and (f) antisepsis
  • Example 70 Synthesis of 2-(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
  • Example 93.1 Assay of inhibition of Escherichia coli RNA polymerase
  • RNA polymerase assays with E. coli RNA polymerase were performed by a modification of the procedure of Kuhlman et al., 2004 [Kuhlman, P., Duff, H. & Galant, A. (2004) A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases. Anal. Biochem. 324, 183-190]. Reaction mixtures contained (20 ⁇ ): 0-100 nM test compound, 75 nM E.
  • coli RNA polymerase ⁇ holoenzyme 20 nM 384 bp DNA fragment containing the bacteriophage T4 N25 promoter, 100 ⁇ ATP, 100 ⁇ GTP, 100 ⁇ UTP, 100 ⁇ CTP, 50 mM Tris-HCl, pH 8.0, 100 mM KCl, 10 mM MgCl 2 , 1 mM DTT, 10 ⁇ g/ml bovine serum albumin, and 5.5% glycerol. Reaction components other than DNA and NTPs were pre- incubated for 10 min at 37°C.
  • Reactions were carried out by addition of DNA and incubation for 5 min at 37°C, followed by addition of NTPs and incubation for 60 min at 37°C.
  • DNA was removed by addition of 1 ⁇ 5 mM CaCl 2 and 2 U DNasel (Ambion, Inc.), followed by incubation for 90 min at 37°C.
  • IC50 is defined as the concentration of inhibitor resulting in 50% inhibition of RNA polymerase activity.
  • Example 93.2 Assay of inhibition of Staphylococcus aureus RNA polymerase
  • RNA polymerase assays with S. aureus RNA polymerase were performed as in Example 93.1, using reaction mixtures containing (20 ⁇ ): 0-100 nM test compound, 75 nM S. aureus RNA polymerase core enzyme, 300 nM S. aureus ⁇ ⁇ , 20 nM 384 bp DNA fragment containing the bacteriophage T4 N25 promoter, 100 ⁇ ATP, 100 ⁇ GTP, 100 ⁇ UTP, 100 ⁇ CTP, 40 mM Tris-HCl, pH 8.0, 80 mM NaCl, 5 mM MgCl 2 , 2.5 mM DTT, and 12.7% glycerol.
  • IC50 is defined as the concentration of inhibitor resulting in 50% inhibition of RNA polymerase activity.
  • Example 94 Assay of inhibition of bacterial growth in culture
  • MICs Minimum inhibitory concentrations for Staphylococcus aureus ATCC 12600, Enterococcus faecalis ATCC 19433, and Escherichia coli D21f2tolC were quantified using spiral gradient endpoint assays, essentially as described [Wallace, A. and Corkill, J. (1989) Application of the spiral plating method to study antimicrobial action. J. Microbiol. Meths. 10, 303-310; Paton, J., Holt, A., and Bywater, M. (1990) Measurement of MICs of antibacterial agents by spiral gradient endpoint compared with conventional dilution methods. Int. J. Exp. Clin. Chemother. 3, 31-38; Schalkowsky S.
  • the streak length was measured using a clear plastic template (Spiral Biotech, Inc.), the test-compound concentration at the streak endpoint was calculated using the program SGE (Spiral Biotech, Inc.), and the MIC was defined as the calculated test-compound concentration at the streak endpoint.
  • Table 3 Inhibition of bacterial growth.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The invention provides compounds that are inhibitors of bacterial RNA polymerase. The invention also provides compositions comprising such compounds, methods of making such compounds, and methods of using said compounds. The invention has applications in control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.

Description

INHIBITORS OF BACTERIAL RNA POLYMERASE:
ARYLPROPANOYL, ARYLPROPENOYL, AND ARYLCYCLOPROPANECARBOXYL PHLOROGLUCINOLS
Government Support
This invention was made with Government support under U19-A1109713 awarded by the National Institutes of Health. The government has certain rights in the invention.
Background
Bacterial infections remain among the most common and deadly causes of human disease. Infectious diseases are the third leading cause of death in the United States and the leading cause of death worldwide (Binder et al. (1999) Science 284, 1311-1313). Multi-drug- resistant bacteria now cause infections that pose a grave and growing threat to public health. It has been shown that bacterial pathogens can acquire resistance to first-line and even second-line antibiotics (Stuart B. Levy, The Challenge of Antibiotic Resistance, in Scientific American, 46- 53 (March, 1998); Walsh, C. (2000) Nature 406, 775-781 ; Schluger, N. (2000) Int. J
Tuberculosis Lung Disease 4, S71-S75; Raviglione et al., (2001) Ann. NY Acad. Sci. 953, 88- 97). New approaches to drug development are necessary to combat the ever-increasing number of antibiotic-resistant pathogens.
RNA polymerase (RNAP) is the molecular machine responsible for transcription and is the target, directly or indirectly, of most regulation of gene expression (Ebright, R. (2000) J. Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 1 1, 155-162; Murakami, K. and Darst, S. (2003) Curr. Opin. Structl. Biol. 13, 31-39; Borukhov, S. and Nudler, E. (2003) Curr. Opin. Microbiol. 6, 93-100; Werner, F. (2007) Mol. Microbiol. 65, 1395-1404; Hirata, A. and Murakami, K. (2009) Curr. Opin. Structl. Biol. 19, 724-731 ; Jun, S., Reichlen, M., Tajiri, M. and Murakami, K. (201 1) Crit. Rev. Biochem. Mol. Biol. 46, 27-40; Cramer, P. (2002) Curr. Opin. Struct. Biol. 12, 89-97; Cramer, P. (2004) Curr. Opin. Genet. Dev. 14, 218-226; Hahn, S. (2004) Nature Struct. Mol. Biol. 11, 394-403; Romberg, R. (2007) Proc. Natl. Acad. Sci. USA 104, 12955-12961 ; Cramer, P., Armache, ., Baumli, S., Benkert, S., Brueckner, F., Buchen, C, Damsma, G., Dengl, S., Geiger, S., Jasiak, A., Jawhari, A., Jennebach, S., Kamenski, T., Kettenberger, Kuhn, C, Lehmann, E., Leike, K., Sydow, J. and Vannini, A. (2008) Annu. Rev. Biophys. 37, 337-352; Lane, W. and Darst, S. (2010) J. Mol. Biol. 395, 671-685; Lane, W. and Darst, S. (2010) J. Mol. Biol. 395, 686-704; Werner, F. and Grohmann, D. (201 1) Nature Rev. Microbiol. 9, 85-98; Vannini, A. and Cramer, P. (2012) Mol. Cell 45, 439-446). Bacterial RNAP core enzyme has a molecular mass of -380,000 Da and consists of one β' subunit, one β subunit, two a subunits, and one ω subunit; bacterial RNAP holoenzyme has a molecular mass of -450,000 Da and consists of bacterial RNAP core enzyme in complex with the transcription initiation factor σ (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 11, 155-162; Cramer, P. (2002) Curr. Opin. Structl. Biol. 12, 89-97; Murakami and Darst (2003) Curr. Opin. Structl. Biol. 13, 31-39; Borukhov and Nudler (2003) Curr. Opin. Microbiol. 6, 93-100). Bacterial RNAP core subunit sequences are conserved across Gram- positive and Gram-negative bacterial species (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 11 , 155-162; Lane, W. and Darst, S. (2010) J Mol. Biol. 395, 671-685; Lane, W. and Darst, S. (2010) J. Mol. Biol. 395, 686-704; ). Eukaryotic RNAP I, RNAP II, and RNAP III contain counterparts of all bacterial RNAP core subunits, but eukaryotic-subunit sequences and bacterial-subunit sequences exhibit only limited conservation (Ebright, R. (2000) J Mol. Biol. 304, 687-698; Darst, S. (2001) Curr. Opin. Structl. Biol. 11, 155-162; Cramer, P. (2002) Curr. Opin. Structl. Biol. 12, 89-97; Cramer, P. (2004) Curr. Opin. Genet. Dev. 14, 218-226; Lane, W. and Darst, S. (2010) J Mol. Biol. 395, 671-685; Lane, W. and Darst, S. (2010) J Mol. Biol. 395, 686-704).
Crystal structures have been determined for bacterial RNAP and eukaryotic RNAP II (Zhang et al., (1999) Cell 98, 811-824; Cramer et al., (2000) Science 288, 640-649; Cramer et al., (2001) Science 292, 1863-1876).
Structures also have been determined for RNAP complexes with nucleic acids, nucleotides and inhibitors (Campbell, et al. (2001) Cell 104, 901-912; Artsimovitch, et al. (2005) Cell 122, 351-363; Campbell, et al. (2005) EMBOJ. 24, 674-682; Tuske, et al. (2005) Cell 122, 541-522; Temiaov, et al. (2005) Mol. Cell 19, 655-666; Mukhopadhyay, J., Das, K., Ismail, S., Koppstein, D., Jang, M., Hudson, B., Sarafianos, S., Tuske, S., Patel, J., Jansen, R., Irschik, H., Arnold, E., and Ebright, R. (2008) Cell 135, 295-307; Belogurov, G., Vassylyeva, M., Sevostyanova, A., Appleman, J., Xiang, A., Lira, R., Webber, S., Klyuyev, S., Nudler, E., Artsimovitch, I., and Vassylyev, D. (2009) Nature. 45, 332-335; Vassylyev, D., Vassylyeva, M., Perederina, A., Tahirov, T. and Artsimovitch, I. (2007) Nature 448, 157-162; Vassylyev, D., Vassylyeva, M., Zhang, J., Palangat, M., Artsimovitch, I. and Landick, R. (2007) Nature 448, 163-168; Gnatt, et al. (2001) Science 292, 1876-1882; Westover, et al. (2004a) Science 303, 1014-1016; Westover, et al. (2004b) Cell 119, 481-489; Ketenberger, et al. (2004) Mol. Cell 16, 955-965; Bushnell, et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1218-1222; Kettenberger, et al. (2005) Natl. Structl. Mol. Biol. 13, 44-48; Ho, M, Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr.
Opin. Structl. Biol. 19, 715-723).
Bacterial RNAP is a proven target for antibacterial therapy (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Chopra, I. (2007) Curr. Opin. Investig. Drugs 8, 600-607; Villain- Guillot, P., Bastide, L., Gualtieri, M. and Leonetti, J. (2007) Drug Discov. Today 12, 200-208; Mariani, R. and Maffioli, S. (2009) Curr. Med. Chem. 16, 430-454; Ho, M, Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin. Structl. Biol. 19, 715-723; Srivastava, A., Talaue, M, Liu, S., Degen, D., Ebright, R.Y., Sineva, E., Chakraborty, A., Druzhinin, S., Chatterjee, S., Mukhopadhyay, J., Ebright, Y., Zozula, A., Shen, J., Sengupta, S., Niedfeldt, R., Xin, C, Kaneko, T., Irschik, H., Jansen, R., Donadio, S., Connell, N. and Ebright, R.H. (2011) Curr. Opin. Microbiol. 14, 532-543). The suitability of bacterial RNAP as a target for antibacterial therapy follows from the fact that bacterial RNAP is an essential enzyme
(permitting efficacy), the fact that bacterial RNAP subunit sequences are conserved (providing a basis for broad-spectrum activity), and the fact that bacterial RNAP subunit sequences are only weakly conserved in eukaryotic RNAP I, RNAP II, and RNAP III (providing a basis for therapeutic selectivity).
The rifamycin antibacterial agents— notably rifampin, rifapentine, and rifabutin— function by binding to and inhibiting bacterial RNAP (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Ho, M., Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin. Structl. Biol. 19, 715-723; Floss and Yu (2005) Chem. Rev. 105, 621 -632; Campbell, et al. (2001) Cell 104, 901 - 912; Artsimovitch, et al. (2005) Cell 122, 351-363; Feklistov, A., Mekler, V., Jiang, Q.,
Westblade, L., Irschik, H., Jansen, R., Mustaev, A., Darst, S., and Ebright, R. (2008) Proc. Natl. Acad. Sci. USA 105, 14820-14825). The rifamycins bind to a site on bacterial RNAP adjacent to the RNAP active center and prevent the extension of RNA chains beyond a length of 2-3 nt.
The rifamycins are in current clinical use in treatment of Gram-positive and Gram-negative bacterial infections (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Ho, M., Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin. Structl. Biol. 19, 715-723; Floss and Yu (2005) Chem. Rev. 105, 621-632; Campbell, et al. (2001) Cell 104, 901-912). The rifamycins are first-line treatments for tuberculosis and are the only current first-line treatments for tuberculosis able to kill non-replicating tuberculosis bacteria, to clear infection, and to prevent relapse (Mitchison, D. (2000) Int. J. Tuberc. Lung Dis. 4, 796-806). The rifamycins also are first-line treatments for biofilm-associated infections of catheters and implanted medical devices and are among the very few current antibacterial drugs able to kill non-replicating biofilm-associated bacteria (Obst, G., Gagnon, R.F., Prentis, J. and Richards, G.K. (1988) ASAIO Trans. 34, 782-784; Obst, G., Gagnon, R.F., Harris, A., Prentis, J. and Richards, G.K. (1989) Am. J. Nephrol. 9, 414-420; Villain-Guillot, P., Gualtieri, M, Bastide, L. and Leonetti, J. P. (2007) Antimicrob. Agents Chemother. 51, 31 17-3121.
The clinical utility of the rifamycin antibacterial agents is threatened by the emergence and spread of bacterial strains resistant to known rifamycins (Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Ho, ML, Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin.
Structl. Biol. 19, 715-723; Floss and Yu (2005) Chem. Rev. 105, 621-632; Campbell, et al. (2001) Cell 104, 901-912). Resistance to rifamycins typically involves substitution of residues in or immediately adjacent to the rifamycin binding site on bacterial RNAP-- i.e., substitutions that directly decrease binding of rifamycins. A significant and increasing percentage of cases of tuberculosis are resistant to rifampicin (1.4% of new cases, 8.7% of previously treated cases, and 100% of cases designated multidrug-resistant, in 1999-2002; Schluger, N. (2000) Int. J. Tuberc. Lung Dis. 4, S71-S75; Raviglione, et al. (2001) Ann. N. Y. Acad. Sci. 953, 88-97; Zumia, et al. (2001) Lancet Infect. Dis. 1, 199-202; Dye, et al. (2002) J Infect. Dis. 185, 1197-1202;
WHO/IUATLD (2003) Anti-tuberculosis drug resistance in the world: third global report (WHO, Geneva)). Strains of bacterial bioweapons agents resistant to rifampicin can be, and have been, constructed (Lebedeva, et al. (1991) Antibiot. Khimioter. 36, 19-22; Pomerantsev, et al. (1993) Antibiot. Khimioter. 38, 34-38; Volger, et al. (2002) Antimicrob. Agents Chemother. 46, 511-513; Marianelli, et al. (204) J Clin. Microbiol. 42, 5439-5443).
In view of the public-health threat posed by rifamycin-resistant bacterial infections, there is an urgent need for new antibacterial agents that target bacterial RNAP and an especially urgent need for new antibacterial agents that target bacterial RNAP derivatives resistant to known rifamycins. (See Darst, S. (2004) Trends Biochem. Sci. 29, 159-162; Chopra, I. (2007) Curr. Opin. Investig. Drugs 8, 600-607; Villain-Guillot, P., Bastide, L., Gualtieri, M. and Leonetti, J. (2007) Drug Discov. Today 12, 200-208; Mariani, R. and Maffioli, S. (2009) Curr. Med. Chem. 16, 430-454; Ho, M., Hudson, B., Das, K., Arnold, E. and Ebright, R. (2009) Curr. Opin.
Structl. Biol. 19, 715-723; Srivastava, A., Talaue, M., Liu, S., Degen, D., Ebright, R.Y., Sineva, E., Chakraborty, A., Druzhinin, S., Chatterjee, S., Mukhopadhyay, J., Ebright, Y., Zozula, A., Shen, J., Sengupta, S., Niedfeldt, R., Xin, C, Kaneko, T., Irschik, H., Jansen, R., Donadio, S., Connell, N. and Ebright, R.H. (2011) Curr. Opin. Microbiol. 14, 532-543.) Summary
The invention provides new compositions of matter—arylpropanoyl phloroglucinols (APPs), arylpropenoyl phloroglucinols (APPUs), and arylcyclopropanecarboxyl phloroglucinols (APPCs)--that inhibit bacterial RNA polymerase and inhibit bacterial growth.
Compounds of the invention differ from previously disclosed arylpropanoyl
phloroglucinols with R AP -inhibitory and antibacterial activities by having a different substituted aryl or heteroaryl moiety and/or a different linker between the aryl or heteroaryl moiety and the phloroglucinol-derived moiety.
It has been determined--surprisingly--that even large substitutions at the "para" position of the aryl or heteroaryl moiety can be tolerated without loss of RNA-polymerase-inhibitory and antibacterial activities.
It has been determined— surprisingly— that the linker between the aryl or heteroaryl moiety and the phloroglucinol-derived moiety can be replaced by a conformationally constrained, olefinic or cyclic linker without loss of RNA-polymerase-inhibitory and antibacterial activities.
Certain compounds of this invention exhibit higher RNA-polymerase-inhibitory and antibacterial potencies than previously disclosed arylpropanoyl phloroglucinols.
Certain compounds of this invention exhibit higher metabolic stabilities than previously disclosed arylpropanoyl phloroglucinols.
Certain compounds of this invention exhibit lower plasma protein binding than previously disclosed arylpropanoyl phloroglucinols.
Certain compounds of this invention exhibit potent RNA-polymerase-inhibitory activities (micromolar and submicromolar IC50s) and potent antibacterial activities (micromolar and submicromolar MICs).
It is believed that compounds of this invention function by binding to the "switch region" of RNA polymerase.
It is believed that compounds of this invention function by binding to the "switch region SW1/SW2 subregion" of RNA polymerase. Applicants have determined crystal structures of complexes containing RNA polymerase and compounds of the invention (Figs. 1-2). Crystals were obtained by soaking 2 (Fig. 1 ; Table 1) or 4 (Fig 2; Table 1) into pre-formed crystals of a complex of Escherichia coli RNA polymerase σ holoenzyme. X-ray diffraction data were collected at a synchrotron light source, structures were solved by molecular replacement, using a previously determined structure of Escherichia coli RNA polymerase σ70 holoenzyme as the search model, and structures were refined to a resolution of 3.8 A and an Rfree of 0.29 (2; Fig. 1) and a resolution of 4.0 A and an Rfree of 0.30 (4; Fig 2).
The structures indicate that compounds of the invention bind to the RNA polymerase switch region SW1/SW2 subregion in a position and orientation generally similar to the binding position and binding orientation of previously disclosed arylpropanoyl phloroglucinols.
This invention provides crystal structures of a complex containing RNA polymerase and 2 and a complex containing RNA polymerase and 4.
Compounds of this invention are anticipated to have applications in analysis of RNA polymerase structure and function, control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, antibacterial therapy, and drug discovery.
In one embodiment the invention provides a compound of the invention which is a compound selected from compounds of structural formulae I)-(VI):
Figure imgf000007_0001
I,
Figure imgf000007_0002
Figure imgf000007_0003
in,
Figure imgf000007_0004
IV
Figure imgf000008_0001
VI,
and tautomers and salts thereof, wherein:
R , R , and R each independently is H, (Ci-C6)alkyl, (C2-C6)alkenyl, or
(Ci-C6)alkoxy, which (Ci-C6)alkyl, (C2-C6)alkenyl, or (Ci-C6)alkoxy optionally is substituted with halo;
R1 is fluoromethyl, difluoromethyl, fluoroethyl, difluoroethyl, trifluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy, difluoromethoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, alkaryl, alkheteroaryl, aryloxy, heteroaiyloxy, or benzyloxy, which alkaiyl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, tri fluoromethoxy, (Q-C^alkyl, (C2-C8)alkenyl, (CrC8)alkoxy, aryl, heteroaryl, aryloxy, and heteroaryloxy; and R3, R4, and R5 each independently is H, halo, (Ci-C )alkyl, or (C2-C8)alkenyl, which (Ci-C )alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRaRb, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C1-C8)alkyl,
(C2-C )alkenyl, (Ci-Cg)alkoxy, (Ci-C7)C(=0)NH-, aryl, heteroaryl, aryloxy, and heteroaryloxy; Ra and Rb each independently is H or (C]-C6)alkyl, or Ra and Rb, together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R3, R4, and R5 is other than H; or
R1 is (C2-C8)alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which (C2-C8)alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C]-C8)alkyl, (C2-C8)alkenyl, (C1-C8)alkoxy, aryl, heteroaiyl, aiyloxy, or heteroaryloxy; and R3, R4, and R5 each is H;
X and Y are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
R2, R6, and R7 each independently is H, (C1-C6)alkyl, (C2-C6)alkenyl, or
(C^Cejalkoxy, which (Ci-C6)alkyl, (C2-C )alkenyl, or (Q-C^alkoxy optionally is substituted with halo;
R1 is halo, (Ci-Cg)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaryl, alkaiyl, alkheteroaryl, aiyloxy, heteroaryloxy, or benzyloxy, which (Ci-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaiyl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy,
Figure imgf000009_0001
(C2-C8)alkenyl, (C1-C8)alkoxy, aryl, heteroaiyl, aiyloxy, and heteroaryloxy;
R3, R4, and R5 each independently is H, halo, (Cj-C8)alkyl, or (C2-C8)alkenyl, which (Cj-Cg)alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRaRb, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Q-Cs^lkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, (C1-C7)C(=0)NH-, aryl, heteroaiyl. aiyloxy, and heteroaryloxy; and Ra and Rb each independently is H or (Ci-C6)alkyl, or Ra and Rb, together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino;
R2", R6", and R7 each independently is H, (CrC6)alkyl, (C2-C6)alkenyl, or (Ci-C6)alkoxy, which (Ci-C6)alkyl, (C2-C6)alkenyl, or (Ci-C6)alkoxy optionally is substituted with halo;
R1 is fluoro, bromo, iodo, (C2-C8)alkyl, (C2-C )alkenyl, (C2-C8)alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which
(C2-Cs)alkyl, (C2-C8)alkenyl, (C2-Cs)alkoxy, aryl, heteroaiyl, alkaryl, alkheteroaryl, aiyloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Q-C^alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaiyl, aryloxy, and heteroaryloxy; and R3 , R4 , and R5 each independently is H, halo, (Ci-C8)alkyl, or (C2-C8)alkenyl, which (C1-C8)alkyl, or (C2-C )alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRa Rb , fluoromethyl, difluoromethyl. trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, (Ci-C7)C(=0)NH-, aiyl, heteroaryl, aryloxy, and heteroaryloxy; Ra and Rb each independently is H or (Ci-C6)alkyl, or Ra and Rb , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R3 , R4 , and R5 is other than H; or
R1 is fluoromethyl, difluoromethyl, trifluromethyl, fluoroethyl, difluoroethyl, trifluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy,
difluoromethoxy, trifluoromethoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, aryloxy, heteroaryloxy, alkaryl, or alkheteroaryl, which aryloxy, heteroarlyoxy, alkaiyl, or alkheteroaryl optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl. trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-Cg)alkyl, (C2-C8)alkenyl,
(Ci-C8)alkoxy, aryl, heteroaryl, aryloxy, and heteroaryloxy; and R3 , R4 , and R5 each is H;
X' and Y' are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
2"' 6'" 7"'
R , R , and R each independently is H, (C1-C6)alkyl, (C2-C )alkenyl, or (C!-C6)alkoxy, which (Ci-C6)alkyl, (C2-C6)alkenyl, or (Ci-C6)alkoxy optionally is substituted with halo;
R is halo, (Ci-Cg)alkyl, (C2-C8)alkenyl, (C1-Cg)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, atyloxy, heteroaiyloxy, or benzyloxy, which (C1-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaryl, alkaiyl, alklieteroaryl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Q-C^alkyl, (C2-C8)alkenyl, (Ci-Cg)alkoxy, aryl, heteroaiyl, aryloxy, and heteroaryloxy; and
R3 , R4 , and R5 each independently is H, halo, (C1-C8)alkyl, or (C2-C8)alkenyl, which (C]-Cs)alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRa Rb , fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl, (C[-C8)alkoxy, (Ci-C7)C(=0)NH-, aryl, heteroaryl, aryloxy, or heteroaiyloxy; and R and Rb each independently is H or (Ci-C6)alkyl, or Ra and Rb , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino.
The invention also provides methods for making a compound of the invention.
The invention also provides an assay for inhibition of a RNA polymerase comprising contacting a bacterial RNA polymerase with a compound of the invention.
The invention also provides an assay for antibacterial activity comprising contacting a bacterial RNA polymerase with a compound of the invention.
The invention also provides a use of a compound of the invention to bind to a bacterial RNA polymerase.
The invention also provides a use of a compound of the invention to inhibit a bacterial RNA polymerase.
The invention also provides a use of a compound of the invention to inhibit bacterial gene expression.
The invention also provides a use of a compound of the invention to inhibit bacterial growth.
The invention also provides a use of a compound of the invention to inhibit a bacterial infection.
The invention also provides a composition comprising a compound of the invention or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable vehicle.
The invention also provides a method for inhibiting the growth of bacteria comprising contacting the bacteria with a compound of the invention or a salt thereof.
The invention also provides a method for inhibiting a bacterial RNA polymerase comprising contacting the bacterial RNA polymerase with a compound of the invention or a salt thereof.
The invention also provides a method for treating a bacterial infection in a mammal, e.g., a human, comprising administering to the mammal an effective amount of a compound of the invention or a pharmaceutically acceptable salt thereof.
The invention also provides a compound of the invention or a pharmaceutically acceptable salt thereof for use in the prophylactic or therapeutic treatment of a bacterial infection.
The invention also provides the use of a compound of the invention or a
pharmaceutically acceptable salt thereof for the preparation of a medicament for treating a bacterial infection in a mammal, e.g., a human.
The invention also provides a compound of the invention or a pharmaceutically acceptable salt thereof for use in medical treatment.
These and other aspects of the present invention will be better appreciated by reference to the following drawings and Detailed Description.
Brief Description of the Figures
Fig. 1 shows a crystal structure of a complex containing RNA polymerase and 2 (see Detailed Description of the Invention). 2 is in a stick representation in dark gray. Experimental electron density for 2 is indicated as a gray mesh (F0-Fc omit map). RNA polymerase backbone atoms in and near 2 binding site are shown in a ribbon representation in light gray. RNA polymerase sidechain atoms that contact 2 are shown in a stick representation in light gray and are labelled with the RNA polymerase subunit (β or β') and residue number.
Fig. 2 shows a crystal structure of a complex containing RNA polymerase and 4 (see Detailed Description of the Invention). 4 is in a stick representation in dark gray. Experimental electron density for 4 is indicated as a gray mesh (F0-Fc omit map). RNA polymerase backbone atoms in and near 4 binding site are shown in a ribbon representation in light gray. RNA polymerase sidechain atoms that contact 4 are shown in a stick representation in light gray and are labelled with the RNA polymerase subunit (β or β') and residue number.
Detailed Description
The following definitions are used, unless otherwise indicated. Halo is fluoro, chloro, bromo, or iodo. Alkyl, alkoxy, alkenyl, alkynyl, etc. denote both straight and branched groups; but reference to an individual radical such as propyl embraces only the straight chain radical, a branched chain isomer such as isopropyl being specifically referred to. Aryl denotes a phenyl radical or an ortho-fused bicyclic carbocyclic radical having about nine to ten ring atoms in which at least one ring is aromatic. Heteroaryl encompasses a radical of a monocyclic aromatic ring containing five or six ring atoms consisting of carbon and one to four heteroatoms each selected from the group consisting of non-peroxide oxygen, sulfur, and N(X) wherein X is absent or is H, O, (C1-C4)alkyl, phenyl or benzyl, as well as a radical of an ortho-fused bicyclic heterocycle of about eight to ten ring atoms comprising one to four heteroatoms each selected from the group consisting of non-peroxide oxygen, sulfur, and N(X).
Alkaryl refers to a group -(Cj-C4)alkyl-aryl.
Alkheteroaryl refers to a group -(C1-C4)alkyl-heteroaryl. Aryloxy refers to a group -O-aryl.
Heteroaryloxy refers to a group -O-heteroaryl.
Unless otherwise specified, the term "binds" used herein refers to high-affinity specific binding (i.e., an interaction for which the equilibrium dissociation constant, Kd, is less than about 100 μΜ and preferably is less than about 10 μΜ.
Unless otherwise specified, structures depicted herein are intended to include all stereochemical forms of the structure; i.e., the R and S configurations for each asymmetric center. Therefore, single stereochemical isomers, as well as enantiomeric and diastereomeric mixtures, of the present compounds are within the scope of the invention.
Unless otherwise specified, structures depicted herein are intended to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures, except for the replacement of a hydrogen atom by a deuterium atom or a tritium atom, or except for the replacement of a carbon by a 13C- or 14C- enriched carbon atom, are within the scope of this invention.
Compounds of this invention may exist in tautomeric forms, such as keto-enol tautomers. The depiction of a single tautomer is understood to represent the compound in all of its tautomeric forms.
The term "pharmaceutically acceptable," as used herein, refers to a component that is, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and other mammals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. A "pharmaceutically acceptable salt" means any non-toxic salt that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention.
Acids commonly employed to form pharmaceutically acceptable salts include inorganic acids such as hydrogen bisulfide, hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid and phosphoric acid, as well as organic acids such as para-toluenesulfonic acid, salicylic acid, tartaric acid, bitartaric acid, ascorbic acid, maleic acid, besylic acid, fumaric acid, gluconic acid, glucuronic acid, formic acid, glutamic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, lactic acid, oxalic acid, para-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid and acetic acid, as well as related inorganic and organic acids. Such pharmaceutically acceptable salts thus include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caprate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-l,4-dioate, hexyne-l,6-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, terephthalate, sulfonate, xylene sulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, β- hydroxybutyrate, glycolate, maleate, tartrate, methanesulfonate, propanesulfonate, naphthalene- 1 -sulfonate, naphthalene-2- sulfonate, mandelate and other salts. In one embodiment, pharmaceutically acceptable acid addition salts include those formed with mineral acids such as hydrochloric acid and hydrobromic acid, and especially those formed with organic acids such as maleic acid.
The pharmaceutically acceptable salt may also be a salt of a compound of the present invention having an acidic functional group, such as a carboxylic acid functional group, and a base. Exemplary bases include, but are not limited to, hydroxide of alkali metals including sodium, potassium, and lithium; hydroxides of alkaline earth metals such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, organic amines such as unsubstituted or hydroxyl-substituted mono-, di-, or tri-alkylamines, dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-OH-(Ci-C )-alkylamine), such as N,N-dimethyl-N-(2-hydroxyethyl)amine or tri-(2- hydroxyethyl)amine; N-methyl-D-glucamine; morpholine; thiomorpholine; piperidine;
pyrrolidine; and amino acids such as arginine, lysine, and the like.
Methods to Prepare Compounds of the Invention
Compounds of the invention can be prepared, for example, according to the following synthetic scheme:
Figure imgf000014_0001
Figure imgf000015_0001
Pharmaceutical Preparations and Methods of Administration
In cases where compounds are sufficiently basic or acidic, a salt of a compound of the invention can be useful as an intermediate for isolating or purifying a compound of the invention. Additionally, administration of a compound of the invention as a pharmaceutically acceptable acid or base salt may be appropriate. Examples of pharmaceutically acceptable salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate, citrate, malonate, tartarate, succinate, benzoate, ascorbate, a-ketoglutarate, and a-glycerophosphate. Suitable inorganic salts may also be formed, including hydrochloride, sulfate, nitrate, bicarbonate, and carbonate salts.
Pharmaceutically acceptable salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion. Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
The compound of the invention can be formulated as pharmaceutical compositions and administered to a mammalian host, such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical, or subcutaneous routes.
Thus, the present compounds may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an
assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet. For oral therapeutic administration, the active compound may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. Such compositions and preparations should contain at least 0.1% of active compound. The percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60% of the weight of a given unit dosage form. The amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained.
The tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added. When the unit dosage form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier, such as a vegetable oil or a polyethylene glycol. Various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form. For instance, tablets, pills, or capsules may be coated with gelatin, wax, shellac or sugar and the like. A syrup or elixir may contain the active compound, sucrose or fructose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring such as cherry or orange flavor. Of course, any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non-toxic in the amounts employed. In addition, the active compound may be incorporated into sustained-release preparations and devices.
The active compound may also be administered intravenously or intraperitoneally by infusion or injection. Solutions of the active compound or its salts can be prepared in water, optionally mixed with a nontoxic surfactant. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of
microorganisms.
The pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes. In all cases, the ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage. The liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.
Sterile injectable solutions are prepared by incorporating the active compound in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filter sterilization. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.
For topical administration, the present compounds may be applied in pure form, i.e., when they are liquids. However, it will generally be desirable to administer them to the skin as compositions or formulations, in combination with a dermatologically acceptable carrier, which may be a solid or a liquid.
Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like. Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants. Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use. The resultant liquid compositions can be applied from absorbent pads, used to impregnate bandages and other dressings, or sprayed onto the affected area using pump-type or aerosol sprayers.
Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user. Useful dosages of the compound of the invention can be determined by comparing their in vitro activity, and in vivo activity in animal models.
The amount of the compound, or an active salt or derivative thereof, required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.
In general, however, a suitable dose will be in the range of from about 0.5 to about 150 mg/kg, e.g., from about 10 to about 100 mg/kg of body weight per day, such as 3 to about 75 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 120 mg/kg/day, most preferably in the range of 15 to 90 mg/kg/day.
The compound is conveniently formulated in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form. In one embodiment, the invention provides a composition comprising a compound of the invention formulated in such a unit dosage form.
The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day. The sub-dose itself may be further divided, e.g., into a number of discrete loosely spaced administrations; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye.
The present invention is not to be limited in scope by the specific embodiments describe herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and the accompanying figures. Such modifications are intended to fall within the scope of the appended claims.
Industrial Applicability
Compounds identified according to the target and method of this invention would have applications not only in antibacterial therapy, but also in: (a) identification of bacterial RNAP (diagnostics, environmental-monitoring, and sensors applications), (b) labeling of bacterial RNAP (diagnostics, environmental-monitoring, imaging, and sensors applications), (c) immobilization of bacterial RNAP (diagnostics, environmental- monitoring, and sensors applications), (d) purification of bacterial RNA polymerase (biotechnology applications), (e) regulation of bacterial gene expression (biotechnology applications), and (f) antisepsis
(antiseptics, disinfectants, and advanced-materials applications). The invention will now be illustrated by the following non-limiting Examples.
Examples
Preparation of Representative Compounds of the Invention
Using procedures similar to those described in the Scheme above, the following compounds (1-92) were prepared.
Table 1. Representative Compounds.
Figure imgf000019_0001
Figure imgf000020_0001
Figure imgf000021_0001
Figure imgf000022_0001
Figure imgf000023_0001
Figure imgf000024_0001

Figure imgf000025_0001
Figure imgf000026_0001
Figure imgf000027_0001
Figure imgf000028_0001
Example 1. Synthesis of 3-(5-methylthiophen-2-yl)-l-(2,4,6-trihydroxyphenyl)propan-l- one
To a solution of l-(2,4,6-trihydroxyphenyl)ethanone (50.00 g, 297.35 mmol, Sigma- Aldrich) in DMF (1.00 L) was added NaH (41.60 g, 1.04 mol, 60% dispersion in mineral oil, Sigma- Aldrich) portionwise at 0-10 °C. The mixture was stirred at 25°C for 30 min, chloro(methoxy)methane (83.79 g, 1.04 mol, 79.05 mL, Sigma- Aldrich) was added dropwise and stirred for another 16 hours. The mixture was poured into water (3 L) and extracted with ethyl acetate (2x1 L), the combined organic layers were washed with brine (1L) and dried over anhydrous Na2S04, filtered and evaporated under vacuum to give a black oil. 1 -(2,4,6- tris(methoxymethoxy)phenyl) ethanone (55.0 g, 61% yield) was isolated by silica gel chromatography (eluent, 30% ethyl acetate in hexane). Yield: 35%. MS (ESI): calculated, m/z 279.3 (MH+); found, 300.0. 1H NMR (400MHz, CDC13) 5 = 6.51 (s, 2H), 5.19 - 5.1 1 (m, 6H), 3.51 - 3.42 (m, 9H), 2.54 - 2.42 (m, 3H).
To a solution of l-(2,4,6-tris(methoxymethoxy)phenyl)ethan-l-one (718.6 mg, 2.392 mmol) and 5-methylthiophene-2-carbaldehyde (315.9 mg, 0.27 ml, 2.5 mmol, Aldrich) in MeOH (6.00 mL) was added KOH (210 mg, 3.74 mmol) at room temperature. The reaction mixture was stirred at room temperature overnight. The reaction mixture was concentrated, and then water (20 ml) was added, 1 M HCl was added to neutralize the pH. The mixture was extracted with ethyl acetate (6 x 20 ml) and the organic layer was washed with brine (30 ml). The combined extracts were dried over anhydrous sodium sulfate, filtered and evaporated under vacuum to crude product. (E)-3-(5-methylthiophen-2-yl)-l(2,4,6-tris(methoxymethoxy)- phenyl)prop-2-en-l-one was isolated by silica gel chromatography (eluent, 40% ethyl acetate in hexane) in 77% yield (752.3 mg).
(E)-3-(5-methylthiophen-2-yl)-l(2,4,6-tris(methoxymethoxy)phenyl)prop-2-en-l-one (0.345 g, 0.845 mmol) was dissolved in 8 mL methanol after flushing with N2. Raney Nickel (162.5 uL, 50% slurry in water, ACROS) was added and then hydrogen gas was bubbled in the reaction mixture via a needle. The reaction was stirred at room temperature; after three hours, the reaction was monitored to be mostly complete by TLC. The reaction mixture was filtered with Celite and rinsed with methanol. The organics was concentrated under vacuum and 3-(5-methylthiophen-2-yl)- 1(2,4,6- tris(methoxymethoxy)phenyl)propan-l-one was isolated by silica gel chromatography in 67% yield (0.232 g) as a clean colorless oil.
3 -(5-methylthiophen-2-yl)- 1 (2,4,6-tris(methoxymethoxy)phenyl)propan- 1 -one (130 mg, 0.317 mmol) was dissolved in 4 mL methanol and concentrated HCl (0.2 mL, 37%, Aldrich) was added dropwise. The reaction was stirred at 60°C for 4 hours. After cooling, the reaction mixture was neutralized with 1M NaOH and then extracted with ethyl acetate (3x10 ml). The combined extracts were dried over anhydrous sodium sulfate, filtered and evaporated under vacuum to crude product. The crude product was purified by prep HPLC (reverse phase). 43.5 mg of 3-(5-methylthiophen-2-yl)-l-(2,4,6-trihydroxyphenyl)propan-l-one was obtained as a light reddish oil. Yield: 39%. MS: m/z 305.1, [M +H]+; 1H NMR (500 MHz, d6-acetone); δ 1 1.7 (s, 1),
9.25 (s, 1), 6.63 (d, J= 3.3 Hz, 1), 6.55 (d, J= 2.3 Hz, 1), 5.94 (s, 2), 3.42 (t, J= 7.4 Hz, 2), 3.1 1
(t, J= 7.4 Hz, 2), 2.38 (s, 3).
Example 2. Synthesis of (2-(4-chlorophenyl)cyclopropyl)(2,4,6- trihydroxyphenyI)methanone
To a solution of l-(2,4,6-tris(methoxymethoxy)phenyl)ethan-l-one (509 mg, 1.69 mmol) and 4- chlorobenzaldehyde (0.148 g, 1.05 mmol, Aldrich) in MeOH (4.00 mL) was added KOH (148 mg, 2.64 mmol) at room temperature. The reaction mixture was concentrated, and then water (20 ml) was added, sat. NH4CI (20 mL) was added to neutralize the pH. The mixture was extracted with ethyl acetate (4 x 15 ml) and the organic layer was washed with brine (30 ml). The combined extracts were dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification on silica gel
(Hex/EtOAc) provided the (2-(4-chlorophenyl)cyclopropyl)(2,4,6-tri(methoxymethoxy)phenyl)methanone as a yellow solid in 80% yield (0.367 g).
In a 50 mL round bottom flask, trimethylsulfoxonium iodide (0.227 g, 1.0 mmol, Aldrich) and sodium hydride (41.0 g, 1.0 mmol, 60%) dispersion in mineral oil, Aldrich) were added sequentially to 4.5 mL dry DMSO. The reaction mixture was stirred for 1 hour followed by the addition of the (2-(4- chlorophenyl)cyclopropyl)(2,4,6-tri(methoxymethoxy)phenyl)methanone (0.122 g, 0.24 mmol) in 1.0 mL DMSO dropwise over 1 minute. The resulting mixture was stirred for 8 hours and then the reaction was quenched by pouring into 50 mL sat. NH4CI. The mixture was extracted with ethyl acetate (4x15 mL). The combined extracted were diluted to 100 mL and extracted with sat. NaCl. The organic layer was dried and concentrated. The crude residue was dissolved in 10 mL methanol and concentrated HCl (2 mL, 37%, Aldrich) was added dropwise. The reaction was stirred overnight. The crude reaction was concentrated on a rotary evaporator and dissolved in 8 mL of 50% acetonitrile/water and purified by prep HPLC. (2-(4- chlorophenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was obtained as an off-white solid (18.3 mg, 25% yield). MS: m/z 305.1 [M +H]+; Ή NMR (500 MHz, <¾-DMSO) δ 12.2 (s, 2), 10.4 (s, 1), 7.30 (dd, J = 46.5, 8.1 Hz, 4), 5.80 (s, 2), 3.83 - 3.52 (m, 1), 3.33 (s, 2), 3.12 (s, 1), 2.77 - 2.26 (m, 4), 2.12 (t, J = 6.5 Hz, 1), 1.91 - 1.72 (m, 1), 1.71 - 1.57 (m, 1), 1.49 (dd, J = 10.3, 7.1 Hz, 1). Example 3. Synthesis of 3-(4-(3-chIorophenoxy)phenyl)-l-(2,4,6- trihydroxyphenyl)propan- 1 -one
3 -(4-(3 -chlorophenoxy)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4-(3-chlorophenoxy)benzaldehyde. Yield: 50%. MS: m/z 385.2 [M + H]+, 387.2 [M + 2 + H]+; 1H NMR (500 MHz, d6-acetone) δ 11.9 (br s, 0.4), 1 1.8 (br s, 1), 9.31 (br s, 1), 7.42 (d, J= 8.1 Hz, 1), 7.38 (d, J= 8.1 Hz, 2), 7.16 (d, J= 7.7 Hz, 1), 7.08 - 7.0 (comp, 3), 6.96 (d, J= 8.1 Hz, 1), 5.97 (s, 2), 3.37 (t, J= 7.4 Hz, 2), 3.04 (t, J- 7.5 Hz, 2).
Example 4. Synthesis of 3-(4-(4-chlorophenoxy)phenyl)-l-(2,4,6- trihydroxyphenyI)propan-l-one
3 -(4-(4-chlorophenoxy)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4-(4- chlorophenoxy)benzaldehyde. The title compound was isolated as a white solid. Yield: 19%. MS: m/z 385.2 [M +H]+, 387.2 [M + 2 + H]+; Ή NMR (500 MHz, d6-acetone) δ 11.7 (br s, 2), 9.24 (br s, 1), 7.41 (d, J= 8.5 Hz, 2), 7.36 (d, J= 8.5 Hz, 2), 7.02 (d, J= 8.6 Hz, 2), 7.0 (d, J= 8.6 Hz, 2), 5.99 (s, 2), 3.45 (t, J= 7.7 Hz, 2), 3.03 (t, J= 7.6 Hz, 2).
Example 5. Synthesis of (E)-3-(4-(4-chlorophenoxy)phenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
3-(4-(4-chlorophenoxy)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4-(4- chlorophenoxy)benzaldehyde. Yield: 50%. MS: m/z 383.2 [M +H]+, 385.2 [M + 2 + H]+; 1H NMR (500 MHz, d6-acetone); the compound exists as a mixture of E and Z isomers in the ratio 1 :0.4; δ 12.2 (br s, 0.3), 11.0 (br s, 2), 8.24 (d, J= 15.6 Hz, 1), 7.82 (d, J= 15.6, 1), 7.77 (d, J = 8.5 Hz, 2), 7.65 (d, J= 8.4, Hz, 1), 7.48 (d, J= 8.6, Hz, 2), 7.45 (br s, 0.4), 7.15 (d, J = 8.7, Hz, 2.5), 7.1 1 (d, J= 8.7, Hz, 2.6), 6.07 - 6.03 (m, 0.4), 6.02 (br s, 2), 5.62 (dd, J= 12.8, J= 2.5. Hz, 0.4), 3.33 - 3.16 (m, 1), 2.85 (dd, J= 17.0, J= 2.6 Hz, 1). Example 6. Synthesis of (E)-3-(4-(pyridin-2-yloxy)phenyl)-l-(2,4,6-trihydroxyphenyl)prop-
2-en-l-one
(E)-3-(4-(pyridin-2-yloxy)phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4-(pyridin-2-yloxy)benzaldehyde. The title compound was isolated as a yellow solid. Yield: 40%. MS: m/z 350.4 [M + H]+; 1H NMR (500 MHz, d6-acetone); the compound exists as a mixture of E and Z isomers in the ratio 1 :0.2; δ 12.2 (br s, 0.2), 8.26 (d, J= 15.7 Hz, 1), 8.20 (d, J= 4.0, 1), 8.18 (br s, 0.2), 7.94 - 7.88 (m, 1), 7.84 (d, J= 15.6 Hz, 1), 7.78 (d, J= 8.5, Hz, 2), 7.65 (d, J= 8.3, Hz, 0.4), 7.24 (d, J= 8.3, Hz, 2.3), 7.18 (app t, J= 6.1, Hz, 1), 7.16 - 7.13 (m, 0.2), 7.09 (d, J= 8.2, Hz, 1), 7.06 (br s, 0.2), 6.05 (br s, 0.2), 6.02 (br s, 2), 5.64 (dd, J= 12.7, J = 2.6. Hz, 0.2), 3.32 - 3.21 (m, 0.4), 2.26 - 2.13 (m, 0.4), 2.05 - 1.93 (m, 0.3).
Example 7. Synthesis of (£)-3-(4-benzyloxy)phenyl)-l-(2,4,6-trihydroxyphenyI)prop-2-en- 1-one
(E)-3-(4-benzyloxy)phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4- (benzyloxy)benzaldehyde. The title compound was isolated as a yellow solid. Yield: 40%. MS: m/z 363.4 [M + H]+; Ή NMR (500 MHz, CDC 13): δ 5.13 (s, 2H), 5.85 (s, 2H), 7.04 (m, 2H), 7.32-7.43 (m, 4H), 7.57 (m, 2H), 7.71 (d, J= 15 Hz, 1H), 8.10 (d, J= 15 Hz, 1H).
Example 8. Synthesis of (E)-3-(4-phenoxyphenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l- one
(£)-3-(4-phenoxyphenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4- phenoxybenzaldehyde. The title compound was isolated as a yellow solid. Yield: 60%. MS: m/z 349.4 [M + H]+; Ή NMR (500 MHz, d6-acetone); the compound exists as a mixture of E and Z isomers; δ 12.2 (br s, 1), 7.63 (d, J= 8.6 Hz, 2), 7.49 - 7.42 (m, 2), 7.21 (app t, J= 7.4 Hz, 1), 7.14 - 7.06 (comp, 4), 6.0 (dd, J= 14.3, 2.2 Hz, 2, 5.61 (dd, J= 12.9, 3.0 Hz, 1), 3.29 - 3.20 (m, 1), 2.84 (dd, J= 17.2, 3.1 Hz, 2), 2.13 (s, 2). Example 9. Synthesis of (E)-3-(2,4-bis(trifluoromethyI)phenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
To a solution of l-[2,4,6-tris(methoxymethoxy)phenyl]ethanone (500.00 mg, 1.67 mmol) and 2,4-bis(trifluoromethyl)benzaldehyde (484.24 mg, 2.00 mmol, Sigma-Aldrich) in MeOH (5.00 mL) was added KOH (140.56 mg, 2.51 mmol) at 0°C. The reaction mixture was brought to room temperature and stirred for 16 hours. The mixture was poured into water (20 mL) and extracted with ethyl acetate (2x30 mL), the combined organic layers were washed with brine (20 mL) and dried over anhydrous Na2S04, filtered and evaporated under vacuum to give a yellow oil. The residue was dissolved in MeOH (5.00 mL) and THF (2.00 mL), con. HC1 (0.2 mL) was added dropwise at 20°C and stirred at 20°C for 12 hours. The mixture was neutralized with saturated NaHC03 solution (3 mL) to pH>6. The mixture was poured into water (20 mL) and extracted with ethyl acetate (2x30 mL), the combined organic layers were washed with brine (20 mL) and dried over anhydrous Na2S04, filtered and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi CI 8 150*25*10 urn, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA-Acetonitrile) to afford 326 mg of the title compound as yellowish solid. Yield: 27%. MS (ESI): calculated, m/z 393.1 (MH+); found, 393.0. Ή NMR (400MHz, d4-Methanol) δ = 8.27 (d, J=15.4 Hz, 1H), 8.12 (d, J=8.2 Hz, 1H), 8.06 - 7.96 (m, 3H), 5.89 (s, 2H).
Example 10. Synthesis of 3-(4-(4-fluorophenoxy)phenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3-(4-(4-fluorophenoxy)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one was synthesized as described in Example 1, but 5-methylthiophene-2-carbaldehyde was replaced by 4-(4- fluorophenoxy)benzaldehyde. The title compound was isolated as a colorless oil. Yield: 34%. MS: m/z 369.4 [M + H]+; Ή NMR (500 MHz, d3-acetonitrile); the compound exists as a mixture of isomers and tautomers; δ 7.25 (d, J= 8.5 Hz, 2), 7.15 - 7.08 (m, 2), 7.04 - 6.99 (m, 2), 6.91 (d, J= 8.6 Hz, 2), 6.15 (d, J= 3.0 Hz, 0.6), 5.95 (d, J= 3.0 Hz, 0.6), 5.92 (br s , 0.1), 5.89 (s, 2), 4.41 (s, 1), 3.34 (t, J= 7.7 Hz, 2), 3.38 (app t, J= 7.1 Hz, 0.4), 3.29 - 3.24 (m, 0.5), 2.27 (br s, 2, signal merged with water peak), 2.94 (t, J= 7.7 Hz, 2), 1.07 (app t, J= 7.2 Hz, 0.5). Example 11. Synthesis of (E)-3-(4-(4-fIuorophenoxy)phenyl)-l -(2,4,6- trihy d roxy pheny I)prop-2-en- 1 -one
(E)-3-(2,4-bis(trifluoromethyl)phenyl)-l -(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-fluorophenoxy)benzaldehyde. Yield: 49%. MS (ESI): calculated, m/z 367.1 (MH+); found, 367.1. 1H NMR (400MHz, d6-Acetone) 6 = 12.21 - 1 1.96 (m, 1H), 9.39 (br s, 1H), 8.27 - 8.12 (m, 1H), 7.84 - 7.66 (m, 3H), 7.28 - 7.13 (m, 4H), 7.04 (d, J=8.7 Hz, 2H), 6.00 (s, 2H).
Example 12. Synthesis of (2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)(2,4,6- trihyd roxy phenyl) methanone
(2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone was synthesized as described in Example 2, but 4-chlorobenzaldehyde was replaced by 4-(4- fluorophenoxy)benzaldehyde. Yield: 16%. MS: m/z 381.0 [M + H]+; 1H NMR (500 MHz, d6- acetone); the compound exists as a mixture of isomers and tautomers; δ 7.28 (d, J= 8.6 Hz, 2), 7.22 - 7.15 (m, 2), 7.09 - 7.04 (m, 2), 6.95 (d, J= 8.4 Hz, 2), 5.99 (s, 2), 3.85 - 3.78 (m, 1), 3.42 (t, J= 7.0 Hz, 2), 3.28 (q, J= 7.1 Hz, 2), 2.69 - 2.62 (m, 1), 2.23 (t, J = 7.9 Hz, 2), 2.05 - 1.95 (comp, 2), 1.90 - 1.84 (m, 1), 1.51 - 1.43 (m, 1), 1.08 (t, J= 7.4 Hz, 3).
Example 13. Synthesis of (2-(4-(4-chlorophenoxy)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-(4-chlorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 2, but 4-chlorobenzaldehyde was replaced by 4-(4- chlorophenoxy)benzaldehyde. Yield: 20%. MS: m/z 397.0 [M + H]+; 1H NMR (500 MHz, d3- acetonitrile); the compound exists as a mixture of isomers and tautomers; δ 7.38 (d, J= 8.9 Hz, 2, 7.32 (d, J= 8.6 Hz, 2), 7.07 - 6.97 (m, 4), 5.87 (s, 2), 4.41 (br s, 0.2), 4.26 - 4.21 (m, 1), 3.37 (t, J = 7.1 Hz, 0.4), 3.26 (q, J= 6.8 Hz, 0.5), 3.10 - 3.03 (comp, 2), 2.13 - 2.05 (comp, 4, signal merged with water peak), 2.01 - 1.97 (comp, 2, signal merged with the solvent peak) 1.08 (t, J= 7.5 Hz, 0.5). Example 14. Synthesis of (E)-3-(4-(tri£luoromethyl)phenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3-(4-(trifluoromethyl)phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(trifluoromethyl)benzaldehyde. Yield: 91%. MS (ESI): calculated, m/z 325.1 (MH+); found, 325.0. Ή NMR (400MHz, d6-Acetone) 1H NMR (400MHz, Acetone) δ = 8.35 (d, J=15.7 Hz, 1H), 7.92 (d, J=8.3 Hz, 2H), 7.84 - 7.77 (m, 3H), 6.01 (s, 2H).
Example 15. Synthesis of (E)-3-(4-phenoxy-2-(trifluoromethyl)phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3-(4-phenoxy-2-(trifluoromethyl)phenyl)- 1 -(2,4,6-trihydroxyphenyl)prop-2-en- 1 -one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-phenoxy-2-(trifluoromethyl)benzaldehyde. Yield: 47%. MS (ESI): calculated, m/z 417.4 (MH+); found, 417.1. Ή NMR (400MHz, d6- Acetone) δ = 12.44 - 11.66 (m, 1H), 9.98 - 9.01 (m, 1H), 8.28 - 8.18 (m, 1H), 8.07 (dd, J=2.3, 15.4 Hz, 1H), 8.02 (d, J=8.8 Hz, 1H), 7.57 - 7.45 (m, 2H), 7.37 (d, J=2.6 Hz, 1H), 7.32 - 7.25 (m, 2H), 7.23 - 7.16 (m, 2H), 6.01 (s, 2H).
Example 16. Synthesis of 3-(4-phenoxy-2-(trifluoromethyl)phenyl)-l-(2,4,6- trihydroxypheny l)propan-l -one
To a solution of (E)-3-(4-phenoxy-2-(trifluoromethyl)phenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one (Example 15, 227.3 mg, 545.94 umol) in MeOH (10.00 mL) was added Pd/C (50.00 mg) and stirred at 20°C under H2 (15 psi) for 15 mins. The reaction mixture was filtered and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi C18 150*25* 10 urn, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA-Acetonitrile) to obtain 204.6 mg of 3-(4-phenoxy-2-(trifluoromethyl)- phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l -one as yellowish solid. Yield: 50%. MS (ESI): calculated, m/z 419.1 (MH+); found, 419.1. Ή NMR (400MHz, d6- Acetone) δ = 12.43 - 1 1.38 (m, 1H), 9.89 - 9.33 (m, 1H), 7.60 (d, J=8.4 Hz, 1H), 7.48 - 7.41 (m, 2H), 7.28 (d, J=2.5 Hz, 1H), 7.24 - 7.18 (m, 2H), 7.12 - 7.07 (m, 2H), 5.98 (s, 2H), 3.49 - 3.42 (m, 2H), 3.22 - 3.13 (m, 2H). Example 17. Synthesis of (E)-3-(4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyI)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3 -(4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl)- 1 -(2,4,6-trihydroxyphenyl)prop- 2-en-l-one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-chlorophenoxy)-2-(trifluoromethyl)benzaldehyde. Yield: 52%. MS (ESI): calculated, m/z 451.1 (MH+); found, 451.0. Ή NMR (400MHz, d6- Acetone) δ = 12.35 - 1 1.25 (m, 1H), 9.43 (br s, 1H), 8.28 - 8.17 (m, 1H), 8.12 - 7.99 (m, 2H), 7.55 - 7.46 (m, 2H), 7.42 (d, J=2.5 Hz, 1H), 7.33 (dd, J=2.5, 8.7 Hz, 1H), 7.25 - 7.13 (m, 2H), 6.00 (s, 2H).
Example 18. Synthesis of (E)-3-(4-(4-chlorophenoxy)-2-methylphenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3-(4-(4-chlorophenoxy)-2-methylphenyl)- 1 -(2,4,6-trihydroxyphenyl)prop-2-en- 1 -one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-chlorophenoxy)-2-methylbenzaldehyde. Yield: 55%. MS (ESI): calculated, m/z 397.2 (MH+); found, 397.1. 1H NMR (400MHz, d6- Acetone) δ = 8.20 - 8.11 (m, 1H), 8.10 - 8.01 (m, 1H), 7.75 (d, J=8.5 Hz, 1H), 7.49 - 7.39 (m, 2H), 7.15 - 7.05 (m, 2H), 6.96 (d, J=2.5 Hz, 1H), 6.90 (dd, J=2.6, 8.6 Hz, 1H), 6.00 (s, 2H), 2.49 (s, 3H).
Example 19. Synthesis of (2-(p-tolyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone
To a solution of l-(2,4,6-trihydroxyphenyl)ethanone (50.00 g, 297.35 mmol, Sigma- Aldrich) in DMF (1.00 L) was added NaH (41.60 g, 1.04 mol, 60% dispersion in mineral oil, Sigma- Aldrich) portionwise at 0~10 °C. The mixture was stirred at 25°C for 30 min, chloro(methoxy)methane (83.79 g, 1.04 mol, 79.05 mL, Sigma- Aldrich) was added dropwise and stirred for another 16 hours. The mixture was poured into water (3 L) and extracted with ethyl acetate (2x1 L), the combined organic layers were washed with brine (1L) and dried over anhydrous Na2S04, filtered and evaporated under vacuum to give a black oil. 1 -(2,4,6- tris(methoxymethoxy)phenyl) ethanone (55.0 g, 61% yield) was isolated by silica gel chromatography (eluent, 30% ethyl acetate in hexane). Yield: 35%. MS (ESI): calculated, m/z 301.1 (MH+); found, 300.0. 1H NMR (400MHz, CDC13) 8 = 6.51 (s, 2H), 5.19 - 5.11 (m, 6H), 3.51 - 3.42 (m, 9H), 2.54 - 2.42 (m, 3H).
To a solution of l-[2,4,6-tris(methoxymethoxy)phenyl]ethanone (2.00 g, 6.66 mmol) and 4-methylbenzaldehyde (0.8 g, 6.66 mmol, Sigma-Aldrich) in MeOH (20.00 mL) was added KOH (560.54 mg, 9.99 mmol) at 0°C. The reaction mixture was brought to room temperature and stirred for 16 hours. The mixture was poured into water (20 mL) and extracted with ethyl acetate (2x30 mL), the combined organic layers were washed with brine (20 mL) and dried over anhydrous Na2S04, filtered and evaporated under vacuum to give a yellow oil. (E)-3-(p-tolyl)-l-
(2,4,6-tris(methoxymethoxy)phenyl)prop-2-en-l-one (2.09 g, 78% yield) was isolated by silica gel chromatography (eluent, 50% ethyl acetate in hexane).
To a solution of trimethylsulfoxonium iodate (4.58 g, 20.81 mmol, Sigma- Aldrich) in
DMSO (15.00 mL) was added NaH (832.45 mg, 20.81 mmol, 60% dispersion in mineral oil,
Sigma-Aldrich) at 5°C. After stirred at 20°C for 1 hour, a solution of (E)-3-(p-tolyl)-l -(2,4,6- tris(methoxymethoxy)phenyl)prop-2-en-l-one (1.67 g, 4.16 mmol) in DMSO (15.00 mL) was added. The reaction mixture was stirred for another 15 hours. The mixture was poured into water
(20 mL), extracted with ethyl acetate (30mL), dried over Na2S04 and concentrated to get a yellow oil. The residue was dissolved in MeOH (10.00 mL) and THF (4.00 mL), con. HCl (0.4 mL) was added dropwise at 20°C and stirred at 20°C for 12 hours. The mixture was neutralized with saturated NaHC03 solution (6 mL) to pH>6. The mixture was poured into water (20 mL) and extracted with ethyl acetate (2x30 mL), the combined organic layers were washed with brine
(20 mL) and dried over anhydrous Na2S04, filtered and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi CI 8 150*25* 10 urn, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA-Acetonitrile) to afford 305 mg of the title compound as yellowish solid. Yield: 43%. MS (ESI): calculated, m/z 285.1 (MH+); found,
285.1. Ή NMR (400MHz, d6- Acetone) δ = 12.26 - 11.44 (m, 1H), 10.19 - 8.92 (m, 1H), 7.35 -
6.88 (m, 4H), 6.19 - 5.87 (m, 2H), 3.84 - 3.73 (m, 1H), 2.59 (ddd, J=4.1, 6.4, 9.0 Hz, 1H), 2.29
(s, 3H), 1.82 (ddd, J=3.6, 5.4, 9.0 Hz, 1H), 1.43 (ddd, J=3.6, 6.5, 8.0 Hz, 1H).
Example 20. Synthesis of (2-(2,4-dimethylphenyl)cycIopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(2,4-dimethylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 2,4- dimethylbenzaldehyde. Yield: 44%. MS (ESI): calculated, m/z 299.1 (MH+); found, 299.1. Ή NMR (400MHz, d6- Acetone) δ = 12.07 - 11.60 (m, 1H), 9.36 (br s, 1H), 7.05 (d, J=7.8 Hz, 1H), 6.98 (s, 1H), 6.94 (d, J=7.7 Hz, 1H), 5.97 (s, 2H), 3.65 - 3.53 (m, 1H), 2.60 (br dd, J=8.2, 11.9 Hz, IH), 2.31 (s, 3H), 2.26 (s, 3H), 1.78 (td, J=4.3, 8.7 Hz, IH), 1.47 (dt, J=3.5, 7.4 Hz,
IH).
Example 21. Synthesis of (2-(4-ethoxyphenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-ethoxyphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-ethoxybenzaldehyde. Yield: 36%. MS (ESI): calculated, m/z 315.1 (MH+); found, 315.1. 1H NMR (400MHz, de- Acetone) δ = 12.20 - 1 1.55 (m, 2H), 9.76 - 9.05 (m, IH), 7.28 - 7.17 (m, 2H), 6.97 - 6.85 (m, 2H), 5.97 - 5.86 (m, 2H), 4.25 - 4.12 (m, IH), 4.10 - 3.99 (m, 2H), 3.14 - 3.07 (m, 2H), 2.03 - 1.91 (m, IH), 1.42 - 1.33 (m, 3H).
Example 22. Synthesis of (2-(4-phenoxyphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone
(2-(4-phenoxyphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-phenoxy- benzaldehyde. Yield: 20%. MS (ESI): calculated, m/z 363.1 (MH+); found, 363.0. 1H NMR (400MHz, d6-Acetone) δ = 12.05 - 1 1.59 (m, 2H), 9.42 (br s, IH), 7.43 - 7.35 (m, 2H), 7.30 - 7.23 (m, 2H), 7.17 - 7.10 (m, IH), 7.03 - 6.97 (m, 2H), 6.97 - 6.91 (m, 2H), 5.97 (s, 2H), 3.87 - 3.74 (m, IH), 2.64 (ddd, J=4.1, 6.4, 9.0 Hz, IH), 1.85 (ddd, J=3.8, 5.3, 9.0 Hz, IH), 1.46 (ddd, J=3.8, 6.5, 8.1 Hz, IH).
Example 23. Synthesis of 3-(4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3 -(4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 - one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-chlorophenoxy)-2-(trifluoromethyl)benzaldehyde. Yield: 42%. MS (ESI): calculated, m/z 453.1 (MH+); found, 453.1. 1H NMR (400MHz, d6- Acetone) δ = 11.77 (br s, IH), 7.61 (d, J=8.5 Hz, IH), 7.49 - 7.39 (m, 2H), 7.33 (d, J=2.5 Hz, IH), 7.26 (dd, J=2.6, 8.5 Hz, IH), 7.15 - 7.03 (m, 2H), 5.96 (s, 2H), 3.55 - 3.41 (m, 2H), 3.23 - 3.14 (m, 2H). Example 24. Synthesis of 3-(4-(4-chIorophenoxy)-2-methylphenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3-(4-(4-chlorophenoxy)-2-methylphenyl)- 1 -(2,4,6-trihydroxyphenyl)propan-l -one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-chlorophenoxy)-2-methylbenzaldehyde. Yield: 61%. MS (ESI): calculated, m/z 399.1 (MH+); found, 399.1. 1H NMR (400MHz, d6- Acetone) 8 = 12.24 - 11.55 (m, 2H), 9.37 (br s, IH), 7.43 - 7.33 (m, 2H), 7.25 (d, J=8.3 Hz, IH), 7.05 - 6.94 (m, 2H), 6.87 (d, J=2.4 Hz, IH), 6.80 (dd, J=2.6, 8.3 Hz, IH), 5.96 (s, 2H), 3.43 - 3.33 (m, 2H), 3.02 - 2.97 (m, 2H), 2.35 (s, 3H).
Example 25. Synthesis of (2-(4-(4-chIorophenoxy)-2- (trifluoromethyl)phenyl)cycIopropyl)(2,4,6-trihydroxyphenyl)methanone
(2-(4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-(4-chlorophenoxy)-2-(trifluoromethyl)benzaldehyde.
Yield: 42%. MS (ESI): calculated, m/z 465.1 (MH+); found, 465.1. 1H NMR (400MHz, de- Acetone) δ = 11.76 (br s, 2H), 9.38 (br s, IH), 7.51 - 7.42 (m, 3H), 7.34 (d, J=2.5 Hz, IH), 7.23 (dd, J=2.4, 8.6 Hz, IH), 7.15 - 7.07 (m, 2H), 5.97 (s, 2H), 3.93 - 3.81 (m, IH), 2.98 - 2.85 (m, IH), 1.83 (ddd, J=4.1, 5.2, 9.1 Hz, IH), 1.66 - 1.57 (m, IH).
Example 26. Synthesis of (2-(4-(4-chlorophenoxy)-2-methylphenyl)cycIopropyl)(2,4,6- trihydroxyphenyl)methanone (0MTK90)
(2-(4-(4-chlorophenoxy)-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)- methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-(4-chlorophenoxy)-2-methylbenzaldehyde. Yield: 33%. MS (ESI): calculated, m/z 411.1 (MH+); found, 41 1.1. 1H NMR (400MHz, d6- Acetone) δ = 12.15 - 1 1.51 (m, 2H), 7.44 - 7.36 (m, 2H), 7.22 (d, J=8.4 Hz, IH), 7.05 - 6.97 (m, 2H), 6.89 (d, J=2.3 Hz, IH), 6.80 (dd, J=2.5, 8.4 Hz, IH), 5.99 (s, 2H), 3.71 - 3.59 (m, IH), 2.69 - 2.58 (m, IH), 2.35 (s, 3H), 1.81 (ddd, J=3.7, 5.1, 8.8 Hz, IH), 1.57 - 1.44 (m, IH).
Example 27. Synthesis of (E)-3-(4-(4-fluorophenoxy)-2-methylphenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3 -(4-(4-fluorophenoxy)-2-methylphenyl)- 1 -(2,4,6-trihydroxyphenyl)prop-2-en- 1 -one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-fluorophenoxy)-2-methylbenzaldehyde. Yield: 64%. MS (ESI): calculated, m/z 381.1 (MH4); found, 381.0. JH NMR (400MHz, d6- Acetone) δ = 12.41 - 11.77 (m, 2H), 9.39 (br s, 1H), 8.17 - 8.11 (m, 1H), 8.09 - 8.01 (m, 1H), 7.73 (d, J=8.5 Hz, 1H), 7.27 - 7.18 (m, 2H), 7.18 - 7.09 (m, 2H), 6.91 (d, J=2.5 Hz, 1H), 6.85 (dd, J=2.5, 8.5 Hz, 1H), 6.00 (s, 2H), 2.48 (s, 3H).
Example 28· Synthesis of (E)-3-(4-(4-(trifluoromethyl)phenoxy)phenyI)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3-(4-(4-(trifluoromethyl)phenoxy)phenyl)- 1 -(2,4,6-trihydroxyphenyl)prop-2-en- 1 -one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-(trifluoromethyl)phenoxy)benzaldehyde. Yield: 54%. MS (ESI): calculated, m/ζ ΑΧΊΛ (MH+); found, 417.1. 1H NMR (400MHz, d6- Acetone) δ = 12.46 - 11.81 (m, 1H), 9.50 (br s, 1H), 8.25 (d, J=15.6 Hz, 1H), 7.87 - 7.73 (m, 5H), 7.26 (d, J=8.5 Hz, 2H), 7.22 - 7.17 (m, 2H), 6.01 (s, 2H).
Example 29. Synthesis of (2-(4-(2,4-dichlorophenoxy)phenyl)cyclopropyI)(2,4,6- trihydroxyphenyl) methanone
(2-(4-(2,4-dichlorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone was synthesized as described in Example 2, but 4-chlorobenzaldehyde was replaced by 4-(2,4- dichlorophenoxy)benzaldehyde. Yield: 1.4%. MS: m/z 431.0, [M +H]+; 1H NMR (500 MHz, </6-acetone); δ 10.9 (s, 3), 7.62 (d, J = 2.4 Hz, 1), 7.39 (dd, J=8.8, 2.4Hz, 1), 7.29 (d, J = 8.4 Hz, 2),7.05(d, J = 8.8 Hz, 1), 6.95 (d, J = 8.4 Hz, 2), 5.96 (s, 2), 3.80 (dt, J = 5.6, 4.7 Hz, 1), 2.72 - 2.52 (m, 1), 1.92 - 1.76 (m, 1), 1.45 (dt, J = 6.6, 5.5 Hz, 1).
Example 30. Synthesis of 3-(4-chlorophenyl)-2-methyl-l-(2,4,6-trihydroxyphenyl)propan- 1-one
To a stirred solution of l-(2,4,6-trihydroxyphenyl)propan-l-one (lg, 5.48 mmol) in dry DMF at 0 °C, under N2 was added NaH (0.65g, 27.4 mmol) in dry DMF:toluene (5 mL each) dropwise and stirred at 0 °C for 1 h. Afterwards, MOMCl (1.66 mL, 21.9 mmol) was added slowly and reaction was allowed to stir at rt overnight. Crude reaction was extracted with EtOAc (50 mL) and washed with 10% NaOH. The organic layer was washed with satd. NaHC03 and brine. Then, the organic layer was dried over anhydrous Na2S04 and evaporated under vacuum to yield an oily residue. Product 1 was isolated by chromatography on silica gel. Yield:
50%. MS: m/z 314 [M +H] +.
KOH (0.107g, 1.92mmol) was added to dry methanol (5 mL) under N2 and stirred until KOH disappeared. Then, a solution of 4-chlorobenzaldehyde (0.213 g, 1.51 mmol) and 1 -(2,4,6- tris(methoxymethoxy)phenyl)propan-l-one (0.43g, 1.37 mmol) in dry methanol (5 mL) was added and heated at 40 °C until reaction completion. Crude was concentrated in vacuo, extracted with EtOAc (3X10 mL) and washed with brine. Then, the organic layer was dried over anhydrous Na2S04 and evaporated under vacuum to yield an oily residue. Product was isolated by chromatography on silica gel. Yield: 50%. MS: m/z 436 [M +H]+.
A solution of 3-(4-chlorophenyl)-2-methyl-l-(2,4,6-tris(methoxymethoxy)phenyl)prop-2- en-l-one (0.1 g, 0.22 mmol) in dry MeOH:THF (3 mL each) was purged with N2 and then 0.02 g of Pd/C was added and the mixture was purged with N2. Then, a H2 balloon was attached and the reaction mixture was stirred for 1 h. Afterwards, crude product was filtered and concentrated. No further purification was required and it was used for next reaction. A solution of 3-(4- chlorophenyl)-2-methyl-l-(2,4,6-tris(methoxymethoxy)phenyl)propan-l-one in dry DCM:MeOH (5 mL:l mL) was added 7-TSOH (0.1 g, 5 eq) and allowed to stir at rt overnight. The crude reaction was quenched with water and extracted with EtOAc (3X10 mL). The organic layer was washed with satd. NaHC03, dried over anhydrous Na2S04; and evaporated under vacuum to yield a solid residue. Product was purified by reverse phase HPLC (80% Water: ACN 20%, with 0.1%) TFA, to 100%> ACN over 15 minutes. Compound was obtained as a white solid in 20% yield; MS: calculated, m/z 306 [M +H]+;. 1H NMR (500 MHz, CD3CN) δ 7.26 (d, J= 8.0 Hz, 2), 7.19 (d, J= 8.0 Hz, 2), 5.86 (s, 2), 4.13 (q, J= 7.0 Hz, 1), 3.10 (dd, J= 13.5, 6.0 Hz, 1), 2.56 (dd, J= 14.0, 7.5 Hz, 1), 1.08 (d, J= 6.5 Hz, 3).
Example 31. Synthesis of (E)-3-(2-methyl-4-phenoxyphenyl)-l-(2,4,6- trihydroxyphenyl)prop-2-en-l-one
(E)-3-(2-methyl-4-phenoxyphenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 2-methyl-4-phenoxybenzaldehyde. Yield: 64%. MS (ESI): calculated, m/z 363.1 (MH+); found, 363.1. 1H NMR (400MHz, d6- Acetone) δ = 12.33 - 1 1.72 (m, 2H), 9.35 (br s, 1H), 8.20 - 8.00 (m, 2H), 7.73 (d, J=8.7 Hz, 1H), 7.50 - 7.38 (m, 2H), 7.20 (t, J=7.4 Hz, 1H), 7.09 (dd, J=0.8, 8.6 Hz, 2H), 6.92 (d, J=2.4 Hz, 1H), 6.86 (dd, J=2.4, 8.6 Hz, 1H), 6.00 (s, 2H), 2.48 (s,
3H).
Example 32. Synthesis of (E)-3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)-l -(2,4,6- trihydroxyphenyI)prop-2-en-l-one
(E)-3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)-l-(2,4,6-trihydroxyphenyl)prop- 2-en-l-one was synthesized as described in Example 9, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-fluorophenoxy)-2-(trifluoromethyl)benzaldehyde. Yield: 21%. MS (ESI): calculated, m/z 435.1 (MH+); found, 435.1. lH NMR (400MHz, d6- Acetone) δ = 12.28 - 11.75 (m, 2H), 9.46 (br s, 1H), 8.26 - 8.19 (m, 1H), 8.1 1 - 8.03 (m, 1H), 8.01 (d, J=8.8 Hz, 1H), 7.36 (d, J=2.6 Hz, 1H), 7.31 - 7.21 (m, 5H), 6.01 (s, 2H).
Example 33. Synthesis of 3-(2-methyl-4-phenoxyphenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3-(2-methyl-4-phenoxyphenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 2 -methyl - 4-phenoxybenzaldehyde. Yield: 58%. MS (ESI): calculated, m/z 365.1 (MH+); found, 365.0. 1H NMR (400MHz, d6- Acetone) δ = 12.28 - 1 1.44 (m, 2H), 9.29 (br s, 1H), 7.41 - 7.31 (m, 2H), 7.23 (d, J=8.3 Hz, 1H), 7.11 (t, J=7.4 Hz, 1H), 6.98 (dd, J=0.9, 8.7 Hz, 2H), 6.85 (d, J=2.4 Hz, 1H), 6.78 (dd, J=2.5, 8.3 Hz, 1H), 5.96 (s, 2H), 3.43 - 3.33 (m, 2H), 3.01 - 2.98 (m, 2H), 2.35 (s, 3H).
Example 34. Synthesis of 3-(4-(4-fluorophenoxy)-2-methylphenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3 -(4-(4-fluorophenoxy)-2-methylphenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-fluorophenoxy)-2-methylbenzaldehyde. Yield: 86%. MS (ESI): calculated, m/z 383.1 (MH+); found, 383.1. 1H NMR (400MHz, d6- Acetone) δ = 1 1.81 (br s, 2H), 9.52 - 9.00 (m, 1H), 7.22 (d, J=8.5 Hz, 1H), 7.19 - 7.09 (m, 2H), 7.06 - 6.99 (m, 2H), 6.83 (d, J=2.5 Hz, 1H),
6.76 (dd, J=2.6, 8.2 Hz, 1H), 5.96 (s, 2H), 3.41 - 3.33 (m, 2H), 2.98 (br s, 2H), 2.34 (s, 3H).
Example 35. Synthesis of 3-(4-(4-(trifluoromethyl)phenoxy)phenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3-(4-(4-(trifluoromethyl)phenoxy)phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-(trifluoromethyl)phenoxy)benzaldehyde. Yield: 57%. MS (ESI): calculated, m/z 419.1 (MH+); found, 419.1. 1H NMR (400MHz, d6- Acetone) δ = 1 1.84 (br s, 2H), 9.40 (br s, 1H), 7.71 (d, J=8.7 Hz, 2H), 7.40 (d, J=8.5 Hz, 2H), 7.13 (d, J=8.5 Hz, 2H), 7.09 - 7.03 (m, 2H), 5.97 (s, 2H), 3.48 - 3.41 (m, 2H), 3.04 (d, J=7.9 Hz, 2H).
Example 36. Synthesis of (2-(4-(4-fluorophenoxy)-2-methyIphenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)- methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-(4-fluorophenoxy)-2-methylbenzaldehyde. Yield: 36%. MS (ESI): calculated, m/z 395.1 (MH+); found, 395.0. 1H NMR (400MHz, d6- Acetone) δ = 12.11 - 11.68 (m, 2H), 9.33 (br s, 1H), 7.22 - 7.11 (m, 3H), 7.08 - 7.00 (m, 2H), 6.84 (d, J=2.4 Hz, 1H), 6.75 (dd, J=2.5, 8.4 Hz, 1H), 5.98 (s, 2H), 3.67 - 3.57 (m, 1H), 2.61 (ddd, J=4.5, 6.8, 8.6 Hz, 1H), 2.34 (s, 3H), 1.81 (ddd, J=3.6, 5.1, 8.8 Hz, 1H), 1.55 - 1.46 (m, 1H).
Example 37. Synthesis of 3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one
3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l- one was synthesized as described in Example 16, but 2,4-bis(trifluoromethyl)benzaldehyde was replaced by 4-(4-fluorophenoxy)-2-(trifluoromethyl)benzaldehyde. Yield: 46%. MS (ESI): calculated, m/z 437.1 (MH+); found, 437.1. 1H NMR (400MHz, d6- Acetone) δ = 1 1.85 - 1 1.44 (m, 2H), 9.34 (br s, 1H), 7.58 (d, J=8.5 Hz, 1H), 7.27 (d, J=2.6 Hz, 1H), 7.26 - 7.1 1 (m, 5H), 5.96 (s, 2H), 3.50 - 3.40 (m, 2H), 3.20 - 3.13 (m, 2H). Example 38. Synthesis of (2-(4-methoxyphenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-methoxyphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4- methoxybenzaldehyde. Yield: 19%. MS (ESI): calculated, m/z 301.1 (MH+); found, 301.0. Ή NMR (400MHz, d6- Acetone) δ = 12.05 - 1 1.53 (m, 2H), 9.64 - 9.31 (m, 1H), 7.30 - 7.20 (m, 2H), 6.97 - 6.87 (m, 2H), 5.96 - 5.89 (m, 2H), 4.25 - 4.15 (m, 1H), 3.83 - 3.78 (m, 3H), 3.13 - 3.10 (m, 2H), 1.99 (td, J=6.0, 8.3 Hz, 1H).
Example 39. Synthesis of (2-(2-methyl-4-phenoxyphenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(2-methyl-4-phenoxyphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 2-methyl-4- phenoxybenzaldehyde. Yield: 48%. MS (ESI): calculated, m/z 377.1 (MH+); found, 377.0. Ή NMR (400MHz, d6- Acetone) δ = 12.16 - 1 1.44 (m, 2H), 9.66 - 9.12 (m, 1H), 7.42 - 7.33 (m, 2H), 7.19 (d, J=8.4 Hz, 1H), 7.16 - 7.09 (m, 1H), 6.99 (d, J=8.4 Hz, 2H), 6.86 (s, 1H), 6.79 - 6.74 (m, 1H), 5.98 (s, 2H), 3.67 - 3.56 (m, 1H), 2.62 (br dd, J=8.2, 1 1.8 Hz, 1H), 2.35 (s, 3H), 1.82 (td, J=4.3, 8.8 Hz, 1H), 1.50 (dt, J=3.6, 7.4 Hz, 1H).
Example 40. Synthesis of (E)-l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(2-methyl-4- phenoxypheny l)prop-2-en-l -one
To a solution of (E)-3-(2-methyl-4-phenoxy-phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2- en-l-one (Example 31, 20.00 mg, 55.19 umol) in CHC13 (1.00 mL) was added sulfuryl chloride (8.19 mg, 60.71 umol) and EtOH (20.00 uL), and the mixture was stirring at 20°C for 1 hour. The solvent was evaporated under vacuum. The residue was purified by prep-HPLC
(Phenomenex Synergi CI 8 150*25* 10 urn, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA-Acetonitrile) to afford 15 mg of the title compound as yellow solid.
Yield: 68%. MS (ESI): calculated, m/z 397.1 (MH+); found, 397.0. 1H NMR (400MHz, de- Acetone) δ = 14.07 (br s, 1H), 10.84 (br s, 1H), 9.86 (br s, 1H), 8.13 (d, J=2.6 Hz, 2H), 7.76 (d, J=8.7 Hz, 1H), 7.49 - 7.40 (m, 2H), 7.25 - 7.17 (m, 1H), 7.14 - 7.06 (m, 2H), 6.97 - 6.84 (m, 2H), 6.28 (s, 1H), 2.50 (s, 3H). Example 41. Synthesis of l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(2-methyl-4- phenoxyphenyl)propan-l-one
1 -(3-chloro-2,4,6-trihydroxyphenyl)-3-(2-methyl-4-phenoxyphenyl)propan- 1 -one was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by 3-(2-methyl-4-phenoxyphenyl)-l -(2,4,6- trihydroxyphenyl)propan- 1 -one. Yield: 68%. MS (ESI): calculated, m/z 399.1 (MH ); found, 399.0. 1H NMR (400MHz, d6- Acetone) δ = 13.56 (br s, IH), 10.75 (br s, IH), 10.13 - 9.61 (m, IH), 7.43 - 7.34 (m, 2H), 7.23 (d, J=8.3 Hz, IH), 7.16 - 7.08 (m, IH), 7.02 - 6.95 (m, 2H), 6.85 (d, J=2.5 Hz, IH), 6.78 (dd, J=2.6, 8.2 Hz, IH), 6.24 (s, IH), 3.47 - 3.36 (m, 2H), 3.02 (br d, J=3.8 Hz, 2H), 2.35 (s, 3H).
Example 42. Synthesis of (3-chloro-2,4,6-trihydroxyphenyI)(2-(2-methyl-4- phenoxyphenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(2-methyl-4-phenoxyphenyl)cyclopropyl)methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en- 1 -one was replaced by (2-(2-methyl-4-phenoxyphenyl)cyclopropyl)- (2,4,6-trihydroxyphenyl)methanone. Yield: 23%. MS (ESI): calculated, m/z 41 1.1 (MH+); found, 411.1. 1H NMR (400MHz, d6- Acetone) δ = 14.07 - 13.40 (m, IH), 10.84 (br s, IH), 7.41 - 7.34 (m, 2H), 7.20 (d, J=8.4 Hz, IH), 7.16 - 7.10 (m, IH), 7.02 - 6.97 (m, 2H), 6.86 (d, J=2.5 Hz, IH), 6.77 (dd, J=2.6, 8.4 Hz, IH), 6.29 (s, IH), 3.64 (td, J=4.8, 7.9 Hz, IH), 2.66 (ddd, J=4.5, 7.0, 8.7 Hz, IH), 2.34 (s, 3H), 1.85 (ddd, J=3.6, 5.1, 8.8 Hz, IH), 1.61 - 1.52 (m, IH).
Example 43. (2-(4-(2,4-difluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone
(2-(4-(2,4-dichlorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl) methanone was synthesized as described in Example 2, but 4-chlorobenzaldehyde was replaced by 4-(2,4- difluorophenoxy)benzaldehyde. Yield: 1.4%. MS: 399.1 , [M +H]+; 1H NMR (500 MHz, d6- DMSO); 5 12.2 (s, 2), 10.4 (s, 1), 7.47 (dd, J = 14.2, 5.7 Hz, 1), 7.37 - 7.16 (m, 3), 7.10 (dd, J = 26.0, 18.0 Hz, 1), 6.88 (d, J = 8.3 Hz, 2), 5.99 - 5.61 (m, 2), 3.85 - 3.54 (m, 1), 3.35 (s, 3), 2.67 - 2.31 (m, 3), 1.90 - 1.62 (m, 1), 1.46 (d, J = 1.9 Hz, 1). Example 44. Synthesis of (2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- cyclopropyl)(2,4,6-trihydroxyphenyl)methanone
(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methyl- benzaldehyde was replaced by 4-(4-fluorophenoxy)-2-(trifluoromethyl)benzaldehyde.
Yield: 30%. MS (ESI): calculated, m/z 449.1 (MH+); found, 448.9. lH NMR (400MHz, d6- Acetone) δ = 11.75 (br s, 2H), 9.36 (br s, 1H), 7.43 (d, J=8.5 Hz, 1H), 7.28 (d, J=2.6 Hz, 1H), 7.26 - 7.19 (m, 2H), 7.19 - 7.13 (m, 3H), 5.97 (s, 2H), 3.85 (td, J=5.1 , 8.1 Hz, 1H), 2.98 - 2.86 (m, 1H), 1.83 (ddd, J=4.0, 5.3, 9.1 Hz, 1H), 1.60 (ddd, J=4.0, 6.7, 8.1 Hz, 1H).
Example 45. Synthesis of l-(3-chIoro-2,4,6-trihydroxyphenyI)-3-(4-(4- fluorophenoxy)phenyl)propan-l-one
l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(4-(4-fluorophenoxy)phenyl)propan-l-one was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by 3-(4-(4-fiuorophenoxy)phenyl)-l-(2,4,6- trihydroxyphenyl)propan-l-one. Yield: 53%. MS (ESI): calculated, m/z 403.1 (MH+); found, 403.0. Ή NMR (400MHz, d6- Acetone) δ = 13.55 (br s, 1H), 10.83 (br s, 1H), 9.89 (br s, 1H), 7.34 - 7.29 (m, 2H), 7.20 - 7.12 (m, 2H), 7.07 - 7.00 (m, 2H), 6.95 - 6.90 (m, 2H), 6.26 (s, 1H), 3.49 - 3.41 (m, 2H), 3.01 (d, J=7.9 Hz, 2H).
Example 46. Synthesis of (3-chloro-2,4,6-trihydroxyphenyl)(2-(4-(4- fluorophenoxy)phenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-(4- fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 60%. MS (ESI): calculated, m/z 415.1 (MH+); found, 415.0. 1H NMR (400MHz, d6- Acetone) δ = 13.54 (br s, 1H), 10.66 (br s, 1H), 9.76 (br s, 1H), 7.31 - 7.23 (m, 2H), 7.21 - 7.12 (m, 2H), 7.08 - 7.01 (m, 2H), 6.97 - 6.90 (m, 2H), 6.25 (s, 1H), 3.90 - 3.67 (m, 1H), 2.69 (ddd, J=4.1 , 6.5, 9.0 Hz, 1H), 1.89 (ddd, J=3.9, 5.4, 9.1 Hz, 1H), 1.51 (ddd, J=3.8, 6.6, 8.0 Hz, 1H). Example 47. Synthesis of (2-(4-chloro-2-(trifluoromethyI)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4- chloro-2-(trifluoromethyl)benzaldehyde. Yield: 41%. MS (ESI): calculated, m/z 373.0 (MH+); found, 372.8. 1H NMR (400MHz, d6- Acetone) δ = 11.92 - 1 1.42 (m, 2H), 9.33 (br s, IH), 7.70 (d, J=2.0 Hz, IH), 7.63 (dd, J=2.0, 8.4 Hz, IH), 7.45 (d, J=8.4 Hz, IH), 5.97 (s, 2H), 3.86 (td, J=5.1, 8.0 Hz, IH), 2.98 - 2.78 (m, IH), 1.86 (ddd, J=4.1, 5.4, 9.2 Hz, IH), 1.64 (ddd, J=4.1, 6.7, 8.2 Hz, IH).
Example 48. Synthesis of (E)-l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(4-(4- fluorophenoxy)phenyl)prop-2-en-l-one
(E)- 1 -(3 -chloro-2,4,6-trihydroxyphenyl)-3 -(4-(4-fluorophenoxy)phenyl)prop-2-en- 1 -one was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by (E)-3-(4-(4-fluorophenoxy)phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one. Yield: 19%. MS (ESI): calculated, m/z 401.1 (MH+); found, 401.0. 1H NMR (400MHz, d6- Acetone) δ = 14.07 (br s, IH), 10.91 (br s, IH), 10.02 (br s, IH), 8.20 (d, J=15.6 Hz, IH), 7.84 (d, J=15.7 Hz, IH), 7.78 - 7.70 (m, 2H), 7.28 - 7.13 (m, 4H), 7.09 - 6.99 (m, 2H), 6.30 (s, IH).
Example 49. Synthesis of l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(4-(4-fluorophenoxy)-2- (trifluoromethyl)phenyl)propan-l-one
l-(3-chloro-2,4,6-trihydroxyphenyl)-3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- propan-l-one was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy- phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was replaced by 3-(4-(4-fiuorophenoxy)-2- (trifluoromethyl)phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one. Yield: 56%. MS (ESI): calculated, m/z 471.1 (MH+); found, 470.9. 1H NMR (400MHz, d6- Acetone) δ = 13.52 (br s, IH), 10.86 (br s, IH), 9.98 (br s, IH), 7.59 (d, J=8.5 Hz, IH), 7.28 (d, J=2.6 Hz, IH), 7.25 - 7.18 (m, 3H), 7.18 - 7.12 (m, 2H), 6.27 (s, IH), 3.58 - 3.35 (m, 2H), 3.23 - 3.15 (m, 2H). Example 50. Synthesis of l-(3-chloro-2,4,6-trihydroxyphenyI)-3-(4-(4-fluorophenoxy)-2- methylphenyl)propan-l-one
1 -(3 -chloro-2,4,6-trihydroxyphenyl)-3 -(4-(4-fluorophenoxy)-2-methylphenyl)propan- 1 - one was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l- (2,4,6-trihydroxyphenyl)prop-2-en-l-one was replaced by 3-(4-(4-fluorophenoxy)-2- methylphenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one. Yield: 47%. MS (ESI): calculated, m/z 417.1 (MH+); found, 417.1. lH NMR (400MHz, d6- Acetone) 6 = 13.54 (br s, 1H), 10.80 (br s, 1H), 9.85 (br s, 1H), 7.22 (d, J=8.3 Hz, 1H), 7.18 - 7.12 (m, 2H), 7.06 - 7.00 (m, 2H), 6.83 (d, J=2.5 Hz, 1H), 6.76 (dd, J=2.6, 8.2 Hz, 1H), 6.24 (s, 1H), 3.44 - 3.36 (m, 2H), 3.03 - 2.98 (m, 2H), 2.34 (s, 3H).
Example 51. Synthesis of (3-chIoro-2,4,6-trihydroxyphenyI)(2-(4-(4-fiuorophenoxy)-2- (trifluoromethyl)phenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- cyclopropyl)methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4- phenoxy-phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-(4- fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)-methanone. Yield: 56%. MS (ESI): calculated, m/z 483.1 (MH+); found, 483.0. 1H NMR (400MHz, de- Acetone) δ = 13.51 (br s, 1H), 10.79 (br s, 1H), 9.92 (br s, 1H), 7.45 (d, J=8.7 Hz, 1H), 7.29 (d, J=2.5 Hz, 1H), 7.26 - 7.20 (m, 2H), 7.19 - 7.13 (m, 3H), 6.27 (s, 1H), 4.01 - 3.60 (m, 1H), 1.93 - 1.81 (m, 1H), 1.72 - 1.61 (m, 1H).
Example 52. Synthesis of (3-chloro-2,4,6-trihydroxyphenyl)(2-(4-(4-fluorophenoxy)-2- methylphenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropyl)- methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy- phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-(4-fluorophenoxy)-2- methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 40%). MS (ESI):
calculated, m/z 429.1 (MH+); found, 429.1. Ή NMR (400MHz, d6- Acetone) δ = 13.74 (br s, 1H), 10.87 (br s, 1H), 10.05 (br s, 1H), 7.23 - 7.12 (m, 3H), 7.07 - 7.00 (m, 2H), 6.84 (d, J=2.3 Hz, 1H), 6.75 (dd, J=2.4, 8.4 Hz, 1H), 6.29 (s, 1H), 3.88 - 3.39 (m, 1H), 2.78 - 2.44 (m, 1H), 2.33 (s, 3H), 1.84 (ddd, J=3.8, 5.0, 8.8 Hz, 1H), 1.55 (dt, J=3.7, 7.5 Hz, 1H). Example 53. Synthesis of (2-(4-chloro-2-methylphenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-chloro-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4-chloro-2- methylbenzaldehyde. Yield: 42%. MS (ESI): calculated, m/z 319.1 (MH+); found, 319.0. 1H NMR (400MHz, d6- Acetone) δ = 12.29 - 11.31 (m, 2H), 9.64 - 8.99 (m, IH), 7.25 - 7.12 (m, 3H), 5.98 (s, 2H), 3.61 (td, J=4.9, 7.8 Hz, IH), 2.60 (ddd, J=4.5, 6.8, 8.7 Hz, IH), 2.36 (s, 3H), 1.82 (ddd, J=3.8, 5.2, 8.9 Hz, IH), 1.50 (ddd, J=3.6, 6.9, 8.0 Hz, IH).
Example 54. Synthesis of (3-chloro-2,4,6-trihydroxyphenyl)(2-(4-chloro-2- (trifluoromethyl)phenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropyl)- methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy- phenyl)-l-(2,4,6-trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-chloro-2- (trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 46%. MS (ESI): calculated, m/z 407.0 (MH+); found, 406.7. Ή NMR (400MHz, d6- Acetone) δ = 13.46 (br s, IH), 10.74 (br s, IH), 9.88 (br s, IH), 7.71 (s, IH), 7.65 (br d, J=8.4 Hz, IH), 7.47 (d, J=8.4 Hz, IH), 6.25 (s, IH), 3.94 - 3.80 (m, IH), 2.93 (br s, IH), 1.91 (td, J=4.7, 9.2 Hz, IH), 1.75 - 1.68 (m, IH).
Example 55. Synthesis of (3-chloro-2,4,6-trihydroxyphenyl)(2-(4- chIorophenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-chlorophenyl)cyclopropyl)methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-chlorophenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone. Yield: 37%. MS (ESI): calculated, m/z 339.0 (MH+); found, 338.6. 1H NMR (400MHz, d6- Acetone) δ = 13.54 (br s, IH), 10.70 (br s, IH), 9.92 (br s, IH), 7.38 - 7.23 (m, 4H), 6.25 (s, IH), 3.86 - 3.72 (m, IH), 2.67 (ddd, J=4.1, 6.4, 9.0 Hz, IH), 1.91 (ddd, J=4.0, 5.3, 9.1 Hz, IH), 1.52 (ddd, J=4.0, 6.6, 8.0 Hz, IH). Example 56. Synthesis of (3-chloro-2,4,6-trihydroxyphenyl)(2-(4-chloro-2- methylphenyl)cyclopropyl)methanone
(3-chloro-2,4,6-trihydroxyphenyl)(2-(4-chloro-2-methylphenyl)cyclopropyl)methanone was synthesized as described in Example 40, but (E)-3-(2-methyl-4-phenoxy-phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en-l-one was replaced by (2-(4-chloro-2-methylphenyl)cyclopropyl)- (2,4,6-trihydroxyphenyl)methanone. Yield: 45%. MS (ESI): calculated, m/z 353.0 (MH+); found, 353.0. Ή NMR (400MHz, d6- Acetone) 6 = 13.59 (br s, 1H), 10.73 (br s, 1H), 10.11 - 9.64 (m, 1H), 7.25 - 7.12 (m, 3H), 6.27 (s, 1H), 3.69 - 3.57 (m, 1H), 2.72 - 2.60 (m, 1H), 2.36 (s, 3H), 1.93 - 1.81 (m, 1H), 1.56 (dt, J=3.7, 7.4 Hz, 1H).
Example 57. Synthesis of (2-(2,4-bis(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(2,4-bis(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 2,4- bis(trifluoromethyl)benzaldehyde. Yield: 93%. MS (ESI): calculated, m/z 407.1 (MH+); found, 406.8. 1H NMR (400MHz, d6- Acetone) δ = 11.92 - 11.46 (m, 2H), 9.44 - 9.18 (m, 1H), 8.01 - 7.93 (m, 2H), 7.66 (d, J=8.2 Hz, 1H), 5.97 (s, 2H), 4.00 - 3.89 (m, 1H), 2.98 (br d, J=4.3 Hz, 1H), 1.94 (ddd, J=4.3, 5.4, 9.2 Hz, 1H), 1.74 (ddd, J=4.1, 6.5, 8.3 Hz, 1H).
Example 58. Synthesis of (2-(4-(trifluoromethyl)phenyl)cycIopropyl)(2,4,6- trihydroxyphenyl)methanone
(2-(4-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone was synthesized as described in Example 19, but 4-methylbenzaldehyde was replaced by 4- (trifluoromethyl)benzaldehyde. Yield: 43%. MS (ESI): calculated, m/z 339.1 (MH+); found, 338.7. 1H NMR (400MHz, d6- Acetone) δ = 11.98 - 11.49 (m, 2H), 9.34 (br s, 1H), 7.63 (d, J=8.2 Hz, 2H), 7.47 (d, J=8.3 Hz, 2H), 5.96 (s, 2H), 4.01 - 3.77 (m, 1H), 2.76 - 2.64 (m, 1H), 1.93 (ddd, J=3.9, 5.5, 9.2 Hz, 1H), 1.55 (ddd, J=3.9, 6.4, 8.2 Hz, 1H).
Example 59. Synthesis of 3,5-dihydroxy-2-(2-(4-phenoxyphenyl)cyclopropanecarbonyI)- 4,6,6-tripropylcyclohexa-2,4-dienone
A solution of [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone (Example 22, 20.00 mg, 55.19 umol) and 1-iodopropane (34.80 mg, 204.75 umol, 20.00 uL, Sigma-AldricA) in anhydrous MeOH (1.00 n L) was added NaOMe (9.84 mg, 182.13 umol, 3.30 eq) and stirred at 20°C for 16 hours. The reaction mixture was cooled to 0°C and acidified with 1 M aqueous HCl and then extracted with ethyl acetate (3x1 OmL). The combined organic layers were dried over anhydrous Na2S04 and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi C18 150*25* 10 urn, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA-Acetonitrile) to afford 9 mg of the title compound as yellow solid. Yield: 33%. MS (ESI): calculated, m/z 489.2 (MH+); found, 489.3. 1H NMR (400MHz, d6-Acetone) δ = 7.46 - 7.35 (m, 2H), 7.28 (d, J=8.7 Hz, 2H), 7.13 (t, J=7.4 Hz, 1H), 7.05 - 6.93 (m, 4H), 4.24 - 4.13 (m, 1H), 2.79 - 2.68 (m, 1H), 2.54 - 2.43 (m, 2H), 1.96 - 1.77 (m, 5H), 1.59 - 1.44 (m, 3H), 1.18 - 1.00 (m, 4H), 0.95 (t, J=7.3 Hz, 3H), 0.86 - 0.79 (m, 4H), 0.78 - 0.71 (m, 3H).
Example 60. Synthesis of 3,5-dihydroxy-2-(2-(4-phenoxyphenyl)cyclopropanecarbonyl)- 6,6-dipropylcyclohexa-2,4-dien-lone
A solution of [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone (Example 22, 20.00 mg, 55.19 umol) and 1-iodopropane (34.80 mg, 204.75 umol, 20.00 uL, Sigma-Aldrich) in anhydrous MeOH (1.00 mL) was added NaOMe (9.84 mg, 182.13 umol, 3.30 eq) and stirred at 20°C for 16 hours. The reaction mixture was cooled to 0°C and acidified with 1 M aqueous HCl and then extracted with ethyl acetate (3x1 OmL). The combined organic layers were dried over anhydrous Na2S04 and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi C18 150*25* 10 urn, 60-90% B in 1 1 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1 % TFA-Acetonitrile) to afford 9 mg of the title compound as yellow solid. Yield: 10%. MS (ESI): calculated, m/z 447.6 (MH+); found, 447.2. 1H NMR (400MHz, d6-Acetone) δ = 7.39 - 7.35 (m, 2H), 7.26 (d, J=8.8 Hz, 1H), 7.00 - 6.94 (m, 5H), 5.62 (s, 1H), 4.24 - 4.13 (m, 1H), 2.79 - 2.68 (m, 1H), 2.54 - 2.43 (m, 2H), 1.81 - 1.70 (m, 4H), 1.25 - 1.00 (m, 4H), 0.84 - 0.75 (m, 6H).
Example 61. Synthesis of 4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)phenyl)propanoyI)-3,5- dihydroxycyclohexa-2,4-dienone
To a solution of (E)-3-[4-(4-fluorophenoxy)phenyl]-l-(2,4,6-trihydroxyphenyl)prop-2- en-l-one (Example 60, 200.00 mg, 545.94 umol) in MeOH (10.00 mL) was added Pd/C (50.00 mg) and stirred at 20°C under H2 (15 psi) for 15 mins. The reaction mixture was filtered and evaporated under vacuum. The residue was purified by prep-HPLC (Phenomenex Synergi CI 8 150*25* 10 um, 60-90% B in 11 mins; buffer A = 0.1% TFA-H20, buffer B = 0.1% TFA- Acetonitrile) to obtain 180 mg of 3-(4-(4-fluorophenoxy)phenyl)-l -(2,4,6- trihydroxyphenyl)propan-l-one as yellowish solid. Yield: 74%. MS (ESI): calculated, m/z 361. \ (MH+); found, 367.1. 1H NMR (400MHz, d6- Acetone) δ = 12.21 - 1 1.93 (m, 1H), 1 1.98 - 11.70 (m, 1H), 9.36 (br s, 1H), 7.31 (d, J=8.4 Hz, 2H), 7.22 - 7.10 (m, 2H), 7.09 - 6.98 (m, 2H), 6.93 (d, J=8.4 Hz, 2H), 5.96 (s, 2H), 3.41 (t, J=7.7 Hz, 2H), 3.00 - 2.91 (m, 2H).
4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxycyclohexa-2,4- dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]- (2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were was replaced by 3-(4-(4- fluorophenoxy)phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one and iodoethane. Yield: 32%. MS (ESI): calculated, m/z 453.1 (MH+); found, 453.1. Ή NMR (400MHz, d6-Acetone) δ = 7.34 (d, J=8.4 Hz, 2H), 7.20 - 7.1 1 (m, 2H), 7.07 - 7.00 (m, 2H), 6.95 (d, J=8.4 Hz, 2H), 3.41 - 3.30 (m, 2H), 2.99 - 2.88 (m, 2H), 2.56 (q, J=7.4 Hz, 2H), 2.03 - 1.84 (m, 4H), 1.12 - 0.98 (m, 3H), 0.68 (t, J=7.5 Hz, 6H).
Example 62. Synthesis of 4,6,6-triethyl-2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropane- carbonyI)-3,5-dihydroxycyclohexa-2,4-dienone
4,6,6-triethyl-2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by 4,6,6-triethyl-2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5- dihydroxycyclohexa-2,4-dienone and iodoethane. Yield: 32%. MS (ESI): calculated, m/z 465.2 (MH+); found, 465.2. Ή NMR (400MHz, d6- Acetone) δ = 7.31 - 7.26 (m, 2H), 7.20 - 7.13 (m, 2H), 7.09 - 7.03 (m, 2H), 6.95 (d, J=8.7 Hz, 2H), 4.25 - 4.12 (m, 1H), 2.80 - 2.68 (m, 1H), 2.56 (q, J=7.4 Hz, 2H), 2.04 - 1.77 (m, 6H), 1.57 - 1.45 (m, 1H), 1.15 - 1.01 (m, 3H), 0.76 - 0.58 (m, 7H).
Example 63. Synthesis of 2-(3-(4-(4-fIuorophenoxy)phenyl)propanoyl)-3,5-dihydroxy-6,6- dipropylcyclohexa-2,4-dienone
2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxy-6,6-dipropylcyclohexa-2,4- dienone and 2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone were synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(4- fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 14%. MS (ESI): calculated, m/z 453.2 (MH+); found, 453.1. Ή NM (400MHz, d6-Acetone) δ = 7.36 - 7.28 (m, 2H), 7.20 - 7.11 (m, 2H), 7.07 - 7.00 (m, 2H), 6.97 - 6.90 (m, 2H), 5.75 (s, 1H), 3.38 - 3.29 (m, 2H), 2.95 - 2.88 (m, 1H), 1.96 - 1.85 (m, 2H), 1.78 (dt, J=4.6, 12.3 Hz, 2H), 1.55 - 1.41 (m, 1H), 1.25 - 0.76 (m, 10H).
Example 64. Synthesis of 2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxy- 4,6,6-tripropylcyclohexa-2,4-dienone
2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxy-6,6-dipropylcyclohexa-2,4- dienone and 2-(3-(4-(4-fluorophenoxy)phenyl)propanoyl)-3,5-dihydroxy-4,6,6-tripropyl- cyclohexa-2,4-dienone were synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(4- fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 12%. MS (ESI): calculated, m/z 495.2 (MH+); found, 495.2. 1H NMR (400MHz, d6-Acetone) Ή NMR
(400MHz, Acetone) δ = 7.33 (d, J=8.4 Hz, 2H), 7.20 - 7.11 (m, 2H), 7.08 - 7.00 (m, 2H), 6.98 - 6.90 (m, 2H), 3.41 - 3.31 (m, 2H), 2.99 - 2.88 (m, 1H), 2.58 - 2.45 (m, 2H), 1.99 - 1.79 (m, 3H), 1.55 - 1.43 (m, 2H), 1.19 - 0.98 (m, 4H), 0.98 - 0.90 (m, 3H), 0.84 - 0.75 (m, 6H).
Example 65. Synthesis of 2-(2-(4-(4-fIuorophenoxy)phenyl)cyclopropanecarbonyl)-3,5- dihydroxy-6,6-dipropylcyclohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-6,6- dipropylcyclohexa-2,4-dienone and 2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)- 3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone were synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 1 1%. MS (ESI): calculated, m/z 465.2 (MH+); found, 465.1. 1H NMR (400MHz, d6-Acetone) δ = 7.36 - 7.28 (m, 2H), 7.20 - 7.11 (m, 2H), 7.07 - 7.00 (m, 2H), 6.97 - 6.90 (m, 2H), 5.75 (s, 1H), 3.38 - 3.29 (m, 2H), 2.95 - 2.88 (m, 1H), 1.96 - 1.85 (m, 2H), 1.78 (dt, J=4.6, 12.3 Hz, 2H), 1.55 - 1.41 (m, 1H), 1.25 - 0.76 (m, 10H). Example 66. Synthesis of 2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-tripropy!cyclohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-6,6- dipropylcyclohexa-2,4-dienone and 2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)- 3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone were synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 12%. MS (ESI): calculated, m/z 495.2 (MH+); found, 495.2. 1H NMR (400MHz, d6-Acetone) 1H NMR (400MHz, Acetone) δ = 7.33 (d, J=8.4 Hz, 2H), 7.20 - 7.11 (m, 2H), 7.08 - 7.00 (m, 2H), 6.98 - 6.90 (m, 2H), 3.41 - 3.31 (m, 2H), 2.99 - 2.88 (m, 1H), 2.58 - 2.45 (m, 2H), 1.99 - 1.79 (m, 3H), 1.55 - 1.43 (m, 2H), 1.19 - 0.98 (m, 4H), 0.98 - 0.90 (m, 3H), 0.84 - 0.75 (m, 6H).
Example 67. Synthesis of (E)-3-(4-(4-fluorophenoxy)phenyl)-l-(2,4,6-trihydroxy-3- propylphenyI)prop-2-en-l-one
(E)-3-(4-(4-fluorophenoxy)phenyl)-l-(2,4,6-trihydroxy-3-propylphenyl)prop-2-en-l-one was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6- trihydroxyphenyl)methanone was replaced by (E)-3-(4-(4-fluorophenoxy)phenyl)-l -(2,4,6- trihydroxyphenyl)prop-2-en- 1 -one. Yield: 7%. MS (ESI): calculated, m/z 409.1 (MH+); found, 409.0. 1H NMR (400MHz, d6- Acetone) δ = 12.45 (s, 1H), 9.69 (br s, 1H), 7.66 - 7.54 (m, 2H), 7.25 - 7.17 (m, 2H), 7.16 - 7.10 (m, 2H), 7.09 - 7.04 (m, 2H), 6.08 (s, 1H), 5.55 (dd, J=3.0, 12.9 Hz, 1H), 3.20 (dd, J=12.9, 17.1 Hz, 1H), 2.80 (dd, J=3.0, 17.1 Hz, 1H), 2.61 - 2.48 (m, 3H), 1.55 (sxt, J=7.5 Hz, 2H), 0.94 (t, J=7.4 Hz, 3H).
Example 68. Synthesis of 2-(3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)propanoyl)- 3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
2-(3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)propanoyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by 3-(4-(4- fluorophenoxy)-2-(trifluoromethyl)phenyl)- 1 -(2,4,6-trihydroxyphenyl)propan- 1 -one. Yield: 17%. MS (ESI): calculated, m/z 563.2 (MH+); found, 563.1. 1H NMR (400MHz, d6-Acetone) δ = 7.63 (br d, J=8.5 Hz, 1H), 7.28 (d, J=2.8 Hz, 1H), 7.25 - 7.19 (m, 3H), 7.18 - 7.13 (m, 2H), 3.42 - 3.36 (m, 2H), 3.14 - 3.08 (m, 2H), 2.54 - 2.47 (m, 2H), 1.97 - 1.82 (m, 4H), 1.50 (qd, J=7.2, 14.9 Hz, 2H), 1.18 - 1.01 (m, 4H), 0.99 - 0.92 (m, 3H), 0.84 - 0.76 (m, 6H).
Example 69. Synthesis of 2-(3-(4-(4-fluorophenoxy)-2-methylphenyl)propanoyI)-3,5- dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
2-(3-(4-(4-fluorophenoxy)-2-methylphenyl)propanoyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by 3-(4-(4- fluorophenoxy)-2-methylphenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one. Yield: 23%. MS (ESI): calculated, m/z 509.3 (MH+); found, 509.1. Ή NMR (400MHz, d6- Acetone) δ = 7.28 - 7.22 (m, 1H), 7.18 - 7.12 (m, 2H), 7.06 - 6.99 (m, 2H), 6.84 (d, J=2.4 Hz, 1H), 6.77 (dd, J=2.3, 8.2 Hz, 1H), 3.35 - 3.28 (m, 2H), 2.99 - 2.86 (m, 2H), 2.55 - 2.46 (m, 2H), 2.35 (s, 3H), 2.01 - 1.83 (m, 4H), 1.54 - 1.44 (m, 2H), 1.17 - 1.00 (m, 4H), 0.98 - 0.91 (m, 3H), 0.85 - 0.77 (m, 6H).
Example 70. Synthesis of 2-(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2- (4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)- methanone. Yield: 23%. MS (ESI): calculated, m/z 575.2 (MH+); found, 575.3. 1H NMR (400MHz, d6-Acetone) 6 = 7.35 (br d, J=8.6 Hz, 1H), 7.18 - 6.99 (m, 7H), 4.09 - 4.01 (m, 1H), 3.51 - 3.42 (m, 1H), 2.84 - 2.76 (m, 2H), 2.36 (br t, J=7.6 Hz, 2H), 1.83 - 1.65 (m, 6H), 1.58 - 1.51 (m, 1H), 1.42 - 1.32 (m, 2H), 1.10 - 0.88 (m, 5H), 0.85 - 0.59 (m, 12H).
Example 71. Synthesis of 2-(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropane- carbonyl)-3,5-dihydroxy-4,6,6-tripropylcycIohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(4- fluorophenoxy)-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 18%. MS (ESI): calculated, m/z 521.3 (MH+); found, 521.1. 1H NMR (400MHz, d6- Acetone) δ = 7.23 (d, J=8.4 Hz, IH), 7.19 - 7.13 (m, 2H), 7.08 - 7.02 (m, 2H), 6.85 (d, J=2.4 Hz, IH), 6.78 (dd,
J=2.4, 8.3 Hz, IH), 3.99 - 3.90 (m, IH), 2.69 - 2.58 (m, IH), 2.54 - 2.44 (m, 2H), 2.31 - 2.24 (m,
3H), 1.96 - 1.79 (m, 5H), 1.61 - 1.47 (m, 3H), 1.21 - 1.03 (m, 4H), 0.99 - 0.92 (m, 3H), 0.85 -
0.76 (m, 6H).
Example 72. Synthesis of 4,6,6-triethyl-2-(2-(4-(4-fluorophenoxy)-2- (trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5-dihydroxycyclohexa-2,4-dienone
4,6,6-triethyl-2-(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropane- carbonyl)-3,5-dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone and iodoethane. Yield: 30%. MS (ESI): calculated, m/z 533.2 (MH+); found, 533.1. 1H NMR (400MHz, d6-Acetone) δ = 7.49 (br d, J=8.5 Hz, IH), 7.28 (d, J=2.5 Hz, IH), 7.26 - 7.16 (m, 5H), 4.28 - 4.14 (m, IH), 2.95 (br s, IH), 2.63 - 2.51 (m, 2H), 1.97 - 1.67 (m, 6H), 1.13 - 1.02 (m, 3H), 0.77 - 0.65 (m, 6H).
Example 73. Synthesis of 4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)- phenyl)propanoyl)-3,5-dihydroxycyclohexa-2,4-dienone
4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)propanoyl)-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by 3-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)- 1 -(2,4,6-trihydroxyphenyl)- propan-l-one and iodoethane. Yield: 20%. MS (ESI): calculated, m/z 521.2 (MH ); found, 521.0. 1H NMR (400MHz, CDC13) δ = 7.46 - 7.35 (m, IH), 7.28 - 7.21 (m, IH), 7.12 - 7.04 (m, 3H), 7.02 - 6.95 (m, 2H), 3.57 - 3.29 (m, 2H), 3.18 - 3.07 (m, 2H), 2.55 - 2.36 (m, 2H), 2.00 - 1.91 (m, 2H), 1.87 - 1.76 (m, 2H), 1.17 - 1.00 (m, 3H), 0.74 - 0.63 (m, 6H).
Example 74. Synthesis of 4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)-2- methyIphenyl)propanoyl)-3,5-dihydroxycycIohexa-2,4-dienone
4,6,6-triethyl-2-(3-(4-(4-fluorophenoxy)-2-methylphenyl)propanoyl)-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by 3-(4-(4-fluorophenoxy)-2-methylphenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one and iodoethane. Yield: 23%. MS (ESI): calculated, m/z 467.2 (MH+); found, 467.1. 1H NMR
(400MHz, d6- Acetone) δ = 7.25 (br d, J=8.3 Hz, IH), 7.18 - 7.11 (m, 2H), 7.05 - 6.99 (m, 2H),
6.83 (d, J=2.1 Hz, IH), 6.79 - 6.72 (m, IH), 3.34 - 3.27 (m, IH), 2.95 - 2.88 (m, 2H), 2.60 - 2.49
(m, 2H), 2.35 (s, 3H), 2.01 - 1.89 (m, 4H), 1.16 - 1.03 (m, 3H), 0.66 (t, J=7.5 Hz, 6H).
Example 75. Synthesis of 2-(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropane- carbonyl)-3,5-dihydroxy-4,6,6-trimethylcyclohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-(4-fluorophenoxy)-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)- methanone and iodomethane. Yield: 44%. MS (ESI): calculated, m/z 437.2 (MH+); found, 437.1. 1H NMR (400MHz, d6-Acetone) δ = 7.22 (d, J=8.4 Hz, IH), 7.19 - 7.13 (m, 2H), 7.08 - 7.02 (m, 2H), 6.84 (d, J=2.0 Hz, IH), 6.77 (dd, J=2.2, 8.3 Hz, IH), 3.84 (br d, J=3.6 Hz, IH), 2.63 (br s, IH), 2.30 (s, 3H), 1.96 - 1.86 (m, 3H), 1.84 - 1.78 (m, IH), 1.54 (br s, IH), 1.39 (s, 6H).
Example 76. Synthesis of 2-(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)- 3,5-dihydroxy-4,6,6-trimethyIcyclohexa-2,4-dienone
2-(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)- methanone and iodomethane. Yield: 18%. MS (ESI): calculated, m/z 415.1 (MH+); found, 414.9. 1H NMR (400MHz, d6-Acetone) δ = 7.75 - 7.65 (m, 2H), 7.52 (br d, J=8.3 Hz, IH), 4.16 - 4.08 (m, IH), 2.93 (br s, IH), 1.94 - 1.80 (m, 4H), 1.72 - 1.65 (m, IH), 1.39 (d, J=1 1.4
Hz, 6H). Example 77. Synthesis of 2-(2-(4-chloro-2-methylphenyl)cycIopropanecarbonyI)-3,5- dihydroxy-4,6,6-trimethylcyclohexa-2,4-dienone
2-(2-(4-chloro-2-methylphenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(4-chloro-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodomethane. Yield: 18%. MS (ESI): calculated, m/z 361.1 (MH+); found, 360.7. 1H NMR (400MHz, d6-Acetone) δ = 7.27 - 7.17 (m, 3H), 3.94 - 3.81 (m, 1H), 2.71 - 2.57 (m, 1H), 2.38 -
2.28 (m, 3H), 1.90 (s, 3H), 1.85 - 1.79 (m, 1H), 1.55 - 1.51 (m, 1H), 1.40 (s, 6H).
Example 78. Synthesis of 2-(3-(4-(4-fluorophenoxy)phenyl)propanoyI)-3,5-dihydroxy- 4,6,6-trimethylcyclohexa-2,4-dienone
2-(3 -(4-(4-fluorophenoxy)phenyl)propanoyl)-3 ,5 -dihydroxy-4,6,6-trimethylcyclohexa- 2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)- cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by 3-(4-(4- fluorophenoxy)phenyl)-l-(2,4,6-trihydroxyphenyl)propan-l-one and iodomethane. Yield: 40%. MS (ESI): calculated, m/z 411.2 (MH+); found, 411.0. 1H NMR (400MHz, d6-Acetone) δ = 7.32 (d, J=8.4 Hz, 2H), 7.19 - 7.11 (m, 2H), 7.08 - 7.00 (m, 2H), 6.94 (d, J=8.5 Hz, 2H), 3.30 (br t, J=7.7 Hz, 2H), 2.92 (br t, J=7.8 Hz, 2H), 1.90 (s, 3H), 1.38 (s, 6H).
Example 79. Synthesis of 2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-3,5-dihydroxy- 4,6,6-tripropyIcyclohexa-2,4-dienone
2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6-tripropylcyclohexa- 2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)- cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-chlorophenyl)- cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 21%. MS (ESI): calculated, m/z 431.2 (MH+); found, 431.0. 1H NMR (400MHz, d6- Acetone) δ = 7.23 - 7.18 (m, 2H), 7.17 - 7.1 1 (m, 2H), 4.1 1 - 3.93 (m, 1H), 2.66 - 2.50 (m, 1H), 2.42 - 2.29 (m, 2H), 1.81 - 1.63 (m, 5H), 1.45 -
1.29 (m, 3H), 1.05 - 0.91 (m, 4H), 0.80 (t, J=7.3 Hz, 3H), 0.70 - 0.65 (m, 3H), 0.63 - 0.55 (m, 3H). Example 80. Synthesis of 2-(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-
3,5-dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
2-(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-chloro- 2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 22%. MS (ESI): calculated, m/z 499.2 (MH+); found, 499.1. 1H NMR (400MHz, d6- Acetone) δ = 7.75 - 7.65 (m, 2H), 7.52 (br d, J=8.3 Hz, 1H), 4.27 - 4.17 (m, 1H), 2.94 (br s, 1H), 2.56 - 2.47 (m, 2H), 1.98 - 1.82 (m, 5H), 1.75 - 1.68 (m, 1H), 1.51 (qd, J=7.4, 15.1 Hz, 2H), 1.19 - 1.03 (m, 4H), 0.95 (t, J=7.3 Hz, 3H), 0.79 (td, J=7.3, 19.8 Hz, 6H).
Example 81. Synthesis of 2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5- dihydroxycyclohexa-2,4-dienone
2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5-dihydroxycyclohexa-2,4- dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]- (2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(4-chlorophenyl)- cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodoethane. Yield: 35%. MS (ESI):
calculated, m/z 389.1 (MH+); found, 389.0. Ή NMR (400MHz, d6- Acetone) δ = 7.38 - 7.33 (m, 2H), 7.32 - 7.27 (m, 2H), 4.23 - 4.15 (m, 1H), 2.76 - 2.69 (m, 1H), 2.62 - 2.50 (m, 2H), 1.99 - 1.82 (m, 5H), 1.55 (ddd, J=3.9, 6.5, 8.2 Hz, 1H), 1.10 - 1.00 (m, 3H), 0.71 (t, J=7.5 Hz, 3H), 0.63 (t, J=7.5 Hz, 3H).
Example 82. Synthesis of 2-(2-(4-chloro-2-methylphenyl)cyclopropanecarbonyl)-4,6,6- triethyl-3,5-dihydroxycyclohexa-2,4-dienone
2-(2-(4-chloro-2-methylphenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(4-chloro-2-methylphenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodoethane. Yield: 24%. MS (ESI): calculated, m/z 403.2 (MH+); found, 403.1. 1H NMR (400MHz, d6-Acetone) δ = 7.26 - 7.18 (m, 3H), 3.99 - 3.92 (m, 1H), 2.67 - 2.62 (m, 1H), 2.61 - 2.53 (m, 2H), 2.33 (s, 3H), 2.00 - 1.84 (m, 5H), 1.61 - 1.54 (m, 1H), 1.08 (t, J=7.4 Hz, 3H), 0.74 - 0.68 (m, 6H). Example 83. Synthesis of 2-(2-(2,4-bis(trifluoromethyl)phenyI)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-trimethylcyclohexa-2,4-dienone
2-(2-(2,4-bis(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(2,4-bis(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodomethane. Yield: 23%. MS (ESI): calculated, m/z 449.1 (MH+); found, 448.9. 1H NMR (400MHz, d6-Acetone) δ = 8.09 - 7.93 (m, 2H), 7.72 (br d, J=8.2 Hz, 1H), 4.30 - 4.08 (m, 1H), 3.02 (br s, 1H), 1.98 - 1.84 (m, 4H), 1.81 - 1.73 (m, 1H), 1.50 - 1.31 (m, 6H).
Example 84. Synthesis of 2-(2-(4-chloro-2-(trifluoromethyI)phenyl)cyclopropanecarbonyl)- 4,6,6-triethyI-3,5-dihydroxycyclohexa-2,4-dienone
2-(2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-chloro-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone and iodoethane. Yield: 41%. MS (ESI): calculated, m/z 457.1 (MH+); found, 457.0. 1H NMR (400MHz, d6-Acetone) δ = 7.60 - 7.46 (m, 2H), 7.37 (br d, J=8.4 Hz, 1H), 4.07 (br s, 1H), 2.79 (br s, 1H), 2.41 (q, J=7.0 Hz, 2H), 1.87 - 1.68 (m, 5H), 1.57 (br s, 1H), 0.92 (br t, J=7.0 Hz, 3H), 0.59 - 0.47 (m, 6H).
Example 85. Synthesis of 2-(2-(4-(4-fluorophenoxy)-2-
(trifluoromethyI)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6-trimethylcycIohexa- 2,4-dienone
2-(2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6- trihydroxyphenyl)methanone and iodomethane. Yield: 23%. MS (ESI): calculated, m/z 491.1 (MH+); found, 491.1. 1H NMR (400MHz, d6- Acetone) δ = 7.48 (br d, J=8.7 Hz, 1H), 7.28 (d, J=2.6 Hz, 1H), 7.25 - 7.21 (m, 2H), 7.21 - 7.19 (m, 1H), 7.19 - 7.15 (m, 2H), 4.13 - 4.06 (m, 1H), 2.93 (br s, 1H), 1.90 (s, 3H), 1.83 (td, J=4.6, 9.0 Hz, 1H), 1.70 - 1.62 (m, 1H), 1.39 (br d,
J=9.4 Hz, 6H).
Example 86. Synthesis of 2-(2-(2,4-bis(trifluoromethyl)phenyI)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-tripropylcyclohexa-2,4-dienone
2-(2-(2,4-bis(trifluoromethyl)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- tripropylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(2,4- bis(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 38%. MS (ESI): calculated, m/z 533.2 (MH+); found, 533.2. 1H NMR (400MHz, d6-Acetone) δ = 7.95 - 7.72 (m, 2H), 7.58 (d, J=8.2 Hz, 1H), 4.15 (br d, J=3.5 Hz, 1H), 2.87 (br s, 1H), 2.36 (br t, J=7.5 Hz, 2H), 1.86 - 1.54 (m, 6H), 1.42 - 1.28 (m, 2H), 1.08 - 0.85 (m, 4H), 0.80 (br t, J=7.2 Hz, 3H), 0.72 - 0.56 (m, 6H).
Example 87. Synthesis of 2-(2-(2,4-bis(trifluoromethyl)phenyl)cyclopropanecarbonyl)- 4,6,6-triethyl-3,5-dihydroxycyclohexa-2,4-dienone
2-(2-(2,4-bis(trifluoromethyl)phenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5- dihydroxycyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(2,4-bis(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodoethane. Yield: 29%. MS (ESI): calculated, m/z 491.2 (MH+); found, 491.1. 1H NMR (400MHz, d6-Acetone) δ = 8.08 - 7.93 (m, 2H), 7.74 (br d, J=8.3 Hz, 1H), 4.38 - 4.23 (m, 1H), 3.04 (br s, 1H), 2.66 - 2.42 (m, 2H), 2.01 - 1.74 (m, 6H), 1.17 - 0.96 (m, 3H), 0.80 - 0.61 (m, 6H).
Example 88. Synthesis of 3,5-dihydroxy-4,6,6-trimethyl-2-(2-(4- (trifluoromethyl)phenyl)cyclopropanecarbonyl)cyclohexa-2,4-dienone
3,5-dihydroxy-4,6,6-trimethyl-2-(2-(4-(trifluoromethyl)phenyl)cyclopropanecarbonyl)- cyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxy- phenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1-iodopropane were replaced by (2-(4-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodomethane. Yield: 30%. MS (ESI): calculated, m/z 381.1 (MH+); found, 381.8. 1H NMR (400MHz, d6- Acetone) δ = 7.67 (br d, J=8.2 Hz, 2H), 7.49 (br d, J=8.2 Hz, 2H), 4.19 - 4.10 (m, IH), 2.83 -
2.75 (m, IH), 1.92 - 1.87 (m, 3H), 1.60 (br d, J=3.1 Hz, IH), 1.51 - 1.44 (m, IH), 1.42 - 1.38 (m,
3H), 1.35 (s, 3H).
Example 89. Synthesis of 2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5- dihydroxycyclohexa-2,4-dienone
2-(2-(4-chlorophenyl)cyclopropanecarbonyl)-4,6,6-triethyl-3,5-dihydroxycyclohexa-2,4- dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl)cyclopropyl]- (2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4- chlorophenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodoethane. Yield: 35%. MS (ESI): calculated, m/z 389.1 (MH+); found, 389.0. 1H NMR (400MHz, d6-Acetone) δ = 7.38 - 7.32 (m, 2H), 7.32 - 7.26 (m, 2H), 4.22 - 4.13 (m, IH), 2.76 - 2.68 (m, IH), 2.55 (q, J=7.4 Hz, 2H), 2.01 - 1.82 (m, 5H), 1.55 (ddd, J=3.9, 6.5, 8.2 Hz, IH), 1.12 - 1.02 (m, 3H), 0.71 (t, J=7.5 Hz, 3H), 0.63 (t, J=7.5 Hz, 3H).
Example 90. Synthesis of 4,6,6-triethyl-3,5-dihydroxy-2-(2-(4- (trifluoromethyl)phenyl)cyclopropanecarbonyl)cyclohexa-2,4-dienone
4,6,6-triethyl-3,5-dihydroxy-2-(2-(4-(trifluoromethyl)phenyl)cyclopropanecarbonyl)- cyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxy- phenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-(trifluoromethyl)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodoethane. Yield: 40%. MS (ESI): calculated, m/z 422.2 (MH+); found, 422.9. 1H NMR (400MHz, de- Acetone) δ = 7.67 (d, J=8.2 Hz, 2H), 7.50 (d, J=8.0 Hz, 2H), 4.35 - 4.20 (m, IH), 2.90 - 2.77 (m, IH), 2.62 - 2.48 (m, 2H), 1.99 - 1.85 (m, 5H), 1.69 - 1.58 (m, IH), 1.17 - 1.00 (m, 3H), 0.72 (t, J=7.4 Hz, 3H), 0.62 (t, J=7.4 Hz, 3H).
Example 91. Synthesis of 3,5-dihydroxy-4,6,6-tripropyl-2-(2-(4- (trifluoromethyl)phenyl)cyclopropanecarbonyl)cyclohexa-2,4-dienone
3,5-dihydroxy-4,6,6-tripropyl-2-(2-(4-(trifluoromethyl)phenyl)cyclopropanecarbonyl)- cyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4-phenoxyphenyl- )cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone was replaced by (2-(4-(trifluoromethyl)- phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone. Yield: 40%. MS (ESI): calculated, m/z 465.2 (MH+); found, 465.2. Ή NMR (400MHz, d6-Acetone) δ = 7.67 (d, J=8.2 Hz, 2H), 7.49 (d, J=8.2 Hz, 2H), 4.31 - 4.15 (m, 1H), 2.85 - 2.75 (m, 1H), 2.59 - 2.43 (m, 2H), 1.96 - 1.76 (m, 5H), 1.62 (ddd, J=4.0, 6.5, 8.4 Hz, 1H), 1.55 - 1.46 (m, 2H), 1.23 - 0.99 (m, 4H), 0.95 (t, J=7.4 Hz, 3H), 0.85 - 0.80 (m, 3H), 0.73 (t, J=7.3 Hz, 3H).
Example 92. Synthesis of 2-(2-(4-(4-fluorophenoxy)phenyI)cyclopropanecarbonyl)-3,5- dihydroxy-4,6,6-trimethylcyclohexa-2,4-dienone
2-(2-(4-(4-fluorophenoxy)phenyl)cyclopropanecarbonyl)-3,5-dihydroxy-4,6,6- trimethylcyclohexa-2,4-dienone was synthesized as described in Example 59, but [2-(4- phenoxyphenyl)cyclopropyl]-(2,4,6-trihydroxyphenyl)methanone and 1 -iodopropane were replaced by (2-(4-(4-fluorophenoxy)phenyl)cyclopropyl)(2,4,6-trihydroxyphenyl)methanone and iodomethane. Yield: 40%. MS (ESI): calculated, m/z 445.2 (M+Na+); found, 445.0. 1H NMR (400MHz, d6-Acetone) δ = 7.27 (br d, J=8.5 Hz, 2H), 7.19 - 7.15 (m, 2H), 7.09 - 7.04 (m, 2H), 6.98 - 6.92 (m, 2H), 4.13 - 3.98 (m, 1H), 2.71 (br s, 1H), 1.92 - 1.80 (m, 3H), 1.60 (br d, J=5.3 Hz, 1H), 1.51 (br s, 1H), 1.43 - 1.29 (m, 6H).
Example 93. Assay of inhibition of bacterial R A polymerase
Example 93.1. Assay of inhibition of Escherichia coli RNA polymerase
Fluorescence-detected RNA polymerase assays with E. coli RNA polymerase were performed by a modification of the procedure of Kuhlman et al., 2004 [Kuhlman, P., Duff, H. & Galant, A. (2004) A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases. Anal. Biochem. 324, 183-190]. Reaction mixtures contained (20 μΐ): 0-100 nM test compound, 75 nM E. coli RNA polymerase σ holoenzyme, 20 nM 384 bp DNA fragment containing the bacteriophage T4 N25 promoter, 100 μΜ ATP, 100 μΜ GTP, 100 μΜ UTP, 100 μΜ CTP, 50 mM Tris-HCl, pH 8.0, 100 mM KCl, 10 mM MgCl2, 1 mM DTT, 10 μg/ml bovine serum albumin, and 5.5% glycerol. Reaction components other than DNA and NTPs were pre- incubated for 10 min at 37°C. Reactions were carried out by addition of DNA and incubation for 5 min at 37°C, followed by addition of NTPs and incubation for 60 min at 37°C. DNA was removed by addition of 1 μΐ 5 mM CaCl2 and 2 U DNasel (Ambion, Inc.), followed by incubation for 90 min at 37°C. RNA was quantified by addition of 100 μΐ RiboGreen RNA Quantitation Reagent (Invitrogen, Inc.; 1 :500 dilution in Tris-HCl, pH 8.0, 1 mM EDTA), followed by incubation for 10 min at 25°C, followed by measurement of fluorescence intensity [excitation wavelength = 485 nm and emission wavelength = 535 nm; QuantaMaster QM1 spectrofluorometer (PTI, Inc.)]. IC50 is defined as the concentration of inhibitor resulting in 50% inhibition of RNA polymerase activity.
Example 93.2. Assay of inhibition of Staphylococcus aureus RNA polymerase
Fluorescence-detected RNA polymerase assays with S. aureus RNA polymerase were performed as in Example 93.1, using reaction mixtures containing (20 μΐ): 0-100 nM test compound, 75 nM S. aureus RNA polymerase core enzyme, 300 nM S. aureus σΛ, 20 nM 384 bp DNA fragment containing the bacteriophage T4 N25 promoter, 100 μΜ ATP, 100 μΜ GTP, 100 μΜ UTP, 100 μΜ CTP, 40 mM Tris-HCl, pH 8.0, 80 mM NaCl, 5 mM MgCl2, 2.5 mM DTT, and 12.7% glycerol. IC50 is defined as the concentration of inhibitor resulting in 50% inhibition of RNA polymerase activity.
Data for representative compounds are shown in Table 2.
Table 2; Inhibition of bacterial RNAP.
Figure imgf000064_0001
14 3.9 7.1
15 2.6 3
16 1.4 1.5
17 1.0 1.3
18 0.61 1.5
19 2.8 1.9
20 0.74 0.64
21 1.3 1.5
22 0.35 0.41
23 0.81 1.2
24 0.99 1.3
25 0.27 0.69
26 0.67 0.79
27 4.3 13
28 1.3 2.8
29 0.53 2.6
30 0.17 5.2
31 2.2 6
32 1.5 3.8
33 1.7 1.6
34 0.72 1.8
35 2.7 3.4
36 0.53 3.2
37 1.7 4.4
38 3.8 3.6
39 0.73 3.5
40 3.9 13
41 1.9 5.1
42 0.38 2.3
43 1.0 4.9
44 0.076 1.2
45 0.11 0.7
46 0.18 1.1
47 0.4 1.0
48 1.6 3.8
49 0.048 0.34
50 0.15 0.35 51 0.042 0.59
52 0.039 0.19
53 1.2 1.7
54 0.67 2.3
55 1.0 2.4
56 0.90 3.4
57 0.60 3.6
58 0.41 3.5
59 0.66 2.3
60 3.2 10
61 1.0 3.0
62 0.87 2.5
63 6.5 >200
64 0.65 6.5
65 >200 >200
66 0.14 1.3
67 12 >200
68 0.019 0.63
69 0.22 3.1
70 0.11 2.2
71 0.13 2.4
72 0.57 14
73 2.4 >22
74 2.2 12
75 0.65 1 1
76 >25 >20
77 >25 8.3
78 2.1 18
79 3.3 11
80 0.58 1.4
81 >50 12
82 1.6 1.3
83 >50 19
84 15 2.8
85 7.5 >30
86 8.4 8.3
87 6.1 21 88 1 1 >40
89 2.1 36
90 4.2 >40
91 0.85 23
92 3.1 >40
Example 94. Assay of inhibition of bacterial growth in culture
Minimum inhibitory concentrations (MICs) for Staphylococcus aureus ATCC 12600, Enterococcus faecalis ATCC 19433, and Escherichia coli D21f2tolC were quantified using spiral gradient endpoint assays, essentially as described [Wallace, A. and Corkill, J. (1989) Application of the spiral plating method to study antimicrobial action. J. Microbiol. Meths. 10, 303-310; Paton, J., Holt, A., and Bywater, M. (1990) Measurement of MICs of antibacterial agents by spiral gradient endpoint compared with conventional dilution methods. Int. J. Exp. Clin. Chemother. 3, 31-38; Schalkowsky S. (1994) Measures of susceptibility from a spiral gradient of drug concentrations. Adv. Exp. Med. Biol. 349, 107-120]. Assays employed exponential-gradient plates containing 150 mm x 4 mm Mueller-Hinton II cation-adjusted agar and 0.4-100 μg/ml of test compound. Plates were prepared using an Autoplate 4000 spiral plater (Spiral Biotech, Inc.). Saturated overnight cultures were swabbed radially onto plates, and plates were incubated for 16 h at 37°C. For each culture, the streak length was measured using a clear plastic template (Spiral Biotech, Inc.), the test-compound concentration at the streak endpoint was calculated using the program SGE (Spiral Biotech, Inc.), and the MIC was defined as the calculated test-compound concentration at the streak endpoint.
Data for representative compounds are shown in Table 3.
Table 3: Inhibition of bacterial growth.
Figure imgf000067_0001
5 2.6 6.0 1.6
6 >40 >40 >40
7 >40 >40 16
8 6.5 31 3.8
9 13 16 8
10 3.1 5.1 1.5
11 17 >40 5.9
12 3.1 4.0 1.5
13 4.3 4.8 2.1
14 32 32 20
15 3.2 3.6 1.8
16 1.1 1.8 1.3
17 1.8 1.9 2.5
18 1.3 2.4 1.1
19 20 >40 13
20 2.1 2.4 1.7
21 16 >40 20
22 1.0 1.3 0.49
23 1.1 1.7 1.7
24 1.8 3.0 1.1
25 1.7 1.7 1.5
26 1.4 2.1 1.4
27 5.1 17 2.4
28 3.1 6.5 2.4
29 1.5 2.4 1.5
30 14 17 6.5
31 5.8 9.6 1.4
32 3.5 5.7 2.1
33 4.6 7.5 1.6
34 3.6 5.2 1.1
35 3.5 5.1 1.3
36 2.4 4.4 1.7
37 3.5 4.4 2.4
38 >40 >40 >40
39 3.6 4.1 1.6
40 >40 >40 9.4
41 19 19 9.4 42 14 16 10
43 6.6 8.4 2.8
44 1.5 1.8 1.5
45 9.6 11 5.2
46 9.6 12 6.6
47 9.6 9.6 4.8
48 >40 >40 20
49 12 12 12
50 16 18 19
51 11 15 16
52 3.2 5.2 7.5
53 4.7 5.3 4.7
54 12 17 17
55 17 22 >40
56 17 22 >40
57 4.3 5.7 3.5
58 10 12 3.9
59 0.7 3.0 1.1
60 10 >40 3.9
61 0.56 0.44 0.24
62 0.40 0.51 0.28
63 5.1 >40 2.4
64 0.26 0.34 0.34
65 1.9 1.9 >40
66 0.16 0.55 0.72
67 0.55 0.72
68 0.6 1.3 1.5
69 1.1 1.4 1.1
70 1.5 1.2 8.2
71 0.9 2.1 2.3
72 6.5 >40 1.6
73 1.5 1.6 1.2
74 1.7 11 0.9
75 17 >40 5.1
76 12 >40 3.1
77 >40 >40 7.4
78 >40 >40 6.5 79 0.27 0.61 0.15
80 0.14 0.42 0.33
81 4.5 1 1 2.2
82 3.6 12 1.2
83 5 >40 2.4
84 1.3 2.1 0.44
85 12 >40 4.0
86 0.65 1 .7 2.2
87 5.9 >40 3.2
88 >40 >40 >40
89 7.9 >40 3.0
90 1.4 2.3 1 .1
91 0.36 0.41 0.66
92 21 >40 5.5
The data in Table 2 show that certain compounds according to general structural formulae (I)-(VI) potently inhibit bacterial RNA polymerases.
The data in Table 3 show that certain compounds according to general structural formulae (I)-(VI) potently inhibit strains of the Gram-positive bacteria Staphylococcus aureus and Enterococcus faecalis and the Gram-negative bacterium Escherichia coli.
All publications, patents, and patent documents are incorporated by reference herein, as though individually incorporated by reference. The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention.

Claims

What is claimed is:
Figure imgf000071_0001
VI, and tautomers and salts thereof, wherein:
R2, R6, and R7 each independently is H, (d-C6)alkyl, (C2-C6)alkenyl, or
(C]-C6)alkoxy, which (Ci-C )alkyl, (C2-C6)alkenyl, or (Ci-C )alkoxy optionally is substituted with halo;
R1 is fluoromethyl, difluoromethyl, fluoroethyl, difluoroethyl, trilluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy, difluoromethoxy, lluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which alkaryl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, tri fluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl, (Ci-C )alkoxy, aryl, heteroaryl, aryloxy, and heteroaryloxy; and R3, R4, and R5 each independently is H, halo, (Ci-C8)alkyl, or (C2-C8)alkenyl, which (Ci-C8)alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRaRb, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C]-C8)alkyl, (C2-C8)alkenyl, (CrC8)alkoxy,
Figure imgf000072_0001
aryl, heteroaryl, aryloxy, and heteroaiyloxy; Ra and Rb each independently is H or (C1-C6)alkyl, or Ra and Rb, together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R3, R4, and R5 is other than H; or
R1 is (C2-C8)alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which (C2-C8)alkenyl, alkaryl, alkheteroaryl, aryloxy, heteroarlyoxy, or benzyloxy optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C]-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaryl, aryloxy, or heteroaryloxy; and R3, R4, and R5 each is H;
X and Y are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
R2', R6, and R7' each independently is H, (Ci-C6)alkyl, (C2-C6)alkenyl, or
(C1-C6)alkoxy, which (Ci-C6)alkyl, (C2-C6)alkenyl, or (Q-C^alkoxy optionally is substituted with halo;
R1 is halo, (C]-C8)alkyl, (C2-C8)alkenyl, (Ci-Cg)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which (Ci-C8)alkyl, (C2-C8)alkenyl, (Ci-Cg)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, aiyloxy, heteroaiyloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
difluoromefhyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Cj-C )alkyl, (C2-Cg)alkenyl, (Ci-Cg)alkoxy, aryl, heteroaryl, aryloxy, and heteroaryloxy;
R3, R4, and R5 each independently is H, halo, (Ci-Cg)alkyl, or (C2-C8)alkenyl, which (Ci-Cs)alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRaRb, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl, (C1-C8)alkoxy, (Ci-C7)C(=0)NH-, aryrl, heteroaryl, aryloxy, and heteroaryloxy; and Ra and Rb each independently is H or (Ci-C6)alkyl, or Ra and Rb, together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino;
2" 6" 7"
R , R , and R each independently is H, (C1-C6)alkyl, (C2-C6)alkenyl, or (Ci-C6)alkoxy, which (C]-C6)alkyl, (C2-C6)alkenyl, or (d-Cejalkoxy optionally is substituted with halo;
R1 is fluoro, bromo, iodo, (C2-C8)alkyl, (C2-Cg)alkenyl, (C2-Cg)alkoxy. aryl, heteroaryl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy, which
(C2-C8)alkyl, (C2-C8)alkenyl, (C2-Cg)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C )alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaryl, aiyloxy, and heteroaryloxy; and R3 , R4 , and R5 each independently is H, halo, (C1-C8)alkyl, or (C2-C8)alkenyl, which (C1-C8)alkyl, or (C2-C8)alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRa Rb , fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-Cg)alkyl, (C2-C8)alkenyl, (C C8)alkoxy, (C1-C7)C(=0)NH-, aryl, heteroatyl, aryloxy, and heteroaiyloxy; Ra and Rb each independently is H or (Ci-C6)alkyl, or Ra and Rb , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino; and at least one of R3 , R4 , and R5 is other than H; or
R1 is fluoromethyl, difluoromethyl, trifluromethyl, fluoroethyl, difluoroethyl, trifluoroethyl, fluoropropyl, difluoropropyl, trifluoropropyl, fluoromethoxy,
difluoromethoxy, trifluoromethoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoropropoxy, difluoropropoxy, trifluoropropoxy, aiyloxy, heteroaiyloxy, alkaryl, or alkheteroaryl, which aryloxy, heteroarlyoxy, alkaryl, alkheteroaryl optionally is substituted by one or more of halo, fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl,
(C]-C8)alkoxy, aryl, heteroaryl, aiyloxy, and heteroaryloxy; and R3 , R4 , and R5 each is
H;
X' and Y' are individually carbon, sulfur, oxygen, or nitrogen, wherein at least one of X and Y is other than carbon;
R2 , R6 , and R7 each independently is H, (Ci-C6)alkyl, (C2-C6)alkenyl, or (Q-C^alkoxy, which (Ci-C6)alkyl, (C2-C6)alkenyl, or (Cj-C6)alkoxy optionally is substituted with halo;
R1 is halo, (Cj-C )alkyl, (C2-C8)alkenyl, (d-Cgjalkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, aiyloxy, heteroaryloxy, or benzyloxy, which (C]-C8)alkyl, (C2-C8)alkenyl, (C]-C8)alkoxy, aryl, heteroaryl, alkaryl, alkheteroaryl, aryloxy, heteroaryloxy, or benzyloxy optionally is substituted with one or more of halo, fluoromethyl,
difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (Ci-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, aryl, heteroaryl, aryloxy, and heteroaryloxy; and
R3 , R4 , and R5 each independently is H, halo, (Cj-C8)alkyl, or (C2-C8)alkenyl, which (C]-C8)alkyl, or (C2-C )alkenyl optionally is substituted with one or more of halo, oxo, hydroxy, carboxy, cyano, -NRa Rb , fluoromethyl, difluoromethyl, trifluromethyl, fluoromethoxy, difluoromethoxy, trifluoromethoxy, (C1-C8)alkyl, (C2-C8)alkenyl, (Ci-C8)alkoxy, (CrC7)C(=0)NH-, aryl, heteroaiyl, aiyloxy, or heteroaryloxy; and Ra and Rb each independently is H or (Ci-Cg)alkyl, or Ra and Rb , together with the nitrogen to which they are attached, form a morpholino, piperazino, pyrrolidino, or piperidino.
2. The compound tautomer or salt of claim 1 which is a compound of formula I or a tautomer or salt thereof.
3. The compound tautomer or salt of claim 1 which is a compound of formula II or or a tautomer or salt thereof.
4. The compound tautomer or salt of claim 1 which is a compound of formula III or or a tautomer or salt thereof.
5. The compound tautomer or salt of claim 1 which is a compound of formula IV or a or a tautomer or salt thereof.
6. The compound tautomer or salt of claim 1 which is a compound of formula V or a tautomer or salt thereof.
7. The compound tautomer or salt of claim 1 which is a compound of formula VI or a tautomer or salt thereof.
8. The compound tautomer or salt of claim 1 which is:
Figure imgf000075_0001
Figure imgf000076_0001
Figure imgf000077_0001
76
Figure imgf000078_0001
77
Figure imgf000079_0001

Figure imgf000080_0001
or a tautomer or salt thereof.
Figure imgf000080_0002
Figure imgf000081_0001
or a tautomer or salt thereof.
10. The compound :
Figure imgf000081_0002
or a tautomer or a salt thereof.
11. Use of a compound, tautomer, or salt as described in any one of claims 1 -10 to bind to a bacterial RNA polymerase.
12. Use of a compound, tautomer, or salt as described in any one of claims 1 -10 to inhibit a bacterial RNA polymerase.
13. Use of a compound, tautomer, or salt as described in any one of claims 1 -10 to inhibit bacterial gene expression.
14. Use of a compound, tautomer, or salt as described in any one of claims 1 -10 to inhibit bacterial growth.
15. Use of a compound, tautomer, or salt as described in any one of claims 1 - 10 to treat a bacterial infection.
16. A composition comprising a compound or tautomer as described in any one of claims 1- 10, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable vehicle.
17. A method for inhibiting a bacterial RNA polymerase comprising contacting the bacterial RNA polymerase with a compound or tautomer as described in any one of claims 1-10, or a salt thereof.
18. A method for inhibiting the growth of bacteria comprising contacting the bacteria with a compound or tautomer as described in any one of claims 1-10, or a salt thereof.
19. A method for treating a bacterial infection in a mammal comprising administering to the mammal an effective amount of a compound or tautomer as described in any one of claims 1-10, or a pharmaceutically acceptable salt thereof.
20. A compound or tautomer as described in any one of claims 1-10, or a pharmaceutically acceptable salt thereof, for use in the prophylactic or therapeutic treatment of a bacterial infection.
21. The use of a compound or tautomer as described in any one of claims 1-10, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for treating a bacterial infection in a mammal.
22. A compound or tautomer as described in any one of claims 1-10, or a pharmaceutically acceptable salt thereof, for use in medical treatment.
23. A method of making a compound, tautomer, or salt as described herein.
24. An invention as described herein.
PCT/US2016/065931 2015-12-10 2016-12-09 Inhibitors of bacterial rna polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols Ceased WO2017100645A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/782,002 US10450292B2 (en) 2015-12-10 2016-12-09 Inhibitors of bacterial RNA polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562265881P 2015-12-10 2015-12-10
US62/265,881 2015-12-10

Publications (2)

Publication Number Publication Date
WO2017100645A2 true WO2017100645A2 (en) 2017-06-15
WO2017100645A3 WO2017100645A3 (en) 2017-07-20

Family

ID=59013643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/065931 Ceased WO2017100645A2 (en) 2015-12-10 2016-12-09 Inhibitors of bacterial rna polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols

Country Status (2)

Country Link
US (1) US10450292B2 (en)
WO (1) WO2017100645A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019173507A1 (en) * 2018-03-06 2019-09-12 Rutgers, The State University Of New Jersey Antibacterial agents : arylalkylcarboxamido phloroglucinols
EP4556004A1 (en) 2023-11-17 2025-05-21 Centre National de la Recherche Scientifique Hop derived compounds for use in the treatment of diseases caused by coronaviruses

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110162028A (en) 2018-02-11 2019-08-23 北京图森未来科技有限公司 A kind of vehicle location shutdown system, method and apparatus
CN118439942B (en) * 2024-04-18 2026-04-21 广东药科大学 A phloroglucinol compound, its preparation method and application

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2898374A (en) 1954-03-01 1959-08-04 Schwarz Lab Inc Nucleus alkylated phloracylophenones and procedure for the production of such compounds
US4061769A (en) 1972-05-24 1977-12-06 Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai Method and composition for treating hypertension
US4350705A (en) 1979-03-31 1982-09-21 Eisai Co., Ltd. Cyclohexane derivatives, process for preparation thereof and medicines containing these cyclohexane derivatives
DE69406475T2 (en) 1993-03-03 1998-03-19 Robertet Sa Use of derivatives of 6,6 dimethyl 2-acylcyclohex 4 en 1,3-diones in sunscreens
IL129871A (en) 1994-05-06 2003-11-23 Pharmacia & Upjohn Inc Process for preparing 4-phenyl-substituted octanoyl-oxazolidin-2-one intermediates that are useful for preparing pyran-2-ones useful for treating retroviral infections
US6022983A (en) 1996-03-22 2000-02-08 Scriptgen Pharmaceuticals, Inc. Pyronin antibacterials, process and novel intermediates thereto
JPH09301915A (en) * 1996-05-08 1997-11-25 Sankyo Co Ltd Flavone and naphthalene derivative
JPH10324657A (en) 1997-05-22 1998-12-08 Hoechst Marion Roussel Kk Polyhydroxybenzene derivatives and prophylactic / therapeutic agents comprising bone and cartilage diseases comprising them
US20030065039A1 (en) * 1997-06-26 2003-04-03 Statens Serum Institute Biologically active 1,3-bis-aromatic-prop-2-en-1-ones, 1,3-bis-aromatic-propan-1-ones, and 1,3-bis-aromatic-prop-2-yn-1-ones
WO2000015626A1 (en) 1998-09-16 2000-03-23 Anadys Pharmaceuticals, Inc. Process for the preparation of intermediates for pyronin antibacterials
JP2005511774A (en) 2001-12-17 2005-04-28 ニュージーランド インスティテュート フォー クロップ アンド フード リサーチ リミティド Antibacterial compounds
US20070292355A1 (en) 2002-10-25 2007-12-20 Foamix Ltd. Anti-infection augmentation foamable compositions and kit and uses thereof
US20050187170A1 (en) 2003-06-16 2005-08-25 Biocryst Pharmaceuticals, Inc. Enhancing the efficiency of RNA polymerase inhibitors by using inosine monophosphate dehydrogenase inhibitors
AU2005243384A1 (en) * 2004-05-17 2005-11-24 Otsuka Pharmaceutical Co., Ltd. Thiazole compound and use thereof
US20060246479A1 (en) 2005-02-10 2006-11-02 Rutgers, The State University Of New Jersey Switch-region: target and method for inhibition of bacterial RNA polymerase
DE102005044156A1 (en) * 2005-09-15 2007-03-29 Riemser Arzneimittel Ag Substituted acetophenone derivatives
US10800725B2 (en) * 2010-09-09 2020-10-13 Richard H. Ebright Arylpropionyl-triketone antibacterial agents
WO2012033815A1 (en) 2010-09-09 2012-03-15 Rutgers, The State University Of New Jersey Arylpropionyl-alpha-pyrone antibacterial agents
WO2012037508A2 (en) 2010-09-17 2012-03-22 Rutgers, The State University Of New Jersey Antibacterial agents: high-potency myxopyronin derivatives
WO2013103969A1 (en) * 2012-01-05 2013-07-11 Rutgers, The State University Of New Jersey Antibacterial agents: phloroglucinol derivatives
US9839634B2 (en) 2012-02-06 2017-12-12 Rutgers, The State University Of New Jersey Antibacterial agents: combination of a rifamycin and a switch region inhibitor
US9187446B2 (en) 2012-03-22 2015-11-17 Rutgers, The State University Of New Jersey Antibacterial agents: sidechainfluorinated myxopyronin derivatives
WO2013192352A1 (en) 2012-06-19 2013-12-27 Rutgers, The State University Of New Jersey Antibacterial agents: aryl myxopyronin derivatives
CN105085219B (en) * 2014-05-22 2019-04-09 中国医学科学院药物研究所 Quinoid chalcone compound with isopentenyl group in A ring, preparation method and anti-inflammatory activity

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019173507A1 (en) * 2018-03-06 2019-09-12 Rutgers, The State University Of New Jersey Antibacterial agents : arylalkylcarboxamido phloroglucinols
US11572337B2 (en) 2018-03-06 2023-02-07 Rutgers, The State University Of New Jersey Antibacterial agents: arylalkylcarboxamido phloroglucinols
EP4556004A1 (en) 2023-11-17 2025-05-21 Centre National de la Recherche Scientifique Hop derived compounds for use in the treatment of diseases caused by coronaviruses
WO2025104281A1 (en) 2023-11-17 2025-05-22 Centre National De La Recherche Scientifique Hop derived compounds for use in the treatment of diseases caused by coronaviruses

Also Published As

Publication number Publication date
US10450292B2 (en) 2019-10-22
US20190023682A1 (en) 2019-01-24
WO2017100645A3 (en) 2017-07-20

Similar Documents

Publication Publication Date Title
EP3842423B1 (en) 3-azabicyclo[3,1,1]heptane derivative and pharmaceutical composition comprising same
JP6482466B2 (en) Cytomegalovirus inhibitors
AU2015313782A1 (en) Compounds and compositions as RAF kinase inhibitors
US9517994B2 (en) Antibacterial agents: phloroglucinol derivatives
US11447502B2 (en) Antibacterial agents: dual-targeted RNA polymerase inhibitors
WO2015120320A1 (en) Antibacterial agents: n(alpha)-aroyl-n-aryl-phenylalaninamides
US9133155B2 (en) Antibacterial agents: high-potency myxopyronin derivatives
US10450292B2 (en) Inhibitors of bacterial RNA polymerase: arylpropanoyl, arylpropenoyl, and arylcyclopropanecarboxyl phloroglucinols
JP2015520186A (en) Dihydronaphthyridine and related compounds useful as kinase inhibitors in the treatment of proliferative diseases
EP2861570A1 (en) Antibacterial agents: aryl myxopyronin derivatives
US9187446B2 (en) Antibacterial agents: sidechainfluorinated myxopyronin derivatives
CA2868002C (en) Antimicrobial agents
WO2012033846A1 (en) Arylpropionyl-triketone antibacterial agents
CN105949181B (en) 4- hydroxypyrrole quinoline -2- ketone derivatives, preparation method and the application of the oxadiazoles containing 1,3,4-
EP1562920A1 (en) Antiinflammatory 3-arylthio-3-thiazolyl-alkylamines
EA018817B1 (en) Tricyclic nitrogen containing compounds and their use as antibacterials
EP3752143B1 (en) Antibacterial agents: o-alkyl-deuterated pyronins
EP3752142A1 (en) Antibacterial agents: soluble salts and aqueous formulations of pyronins
EP0111904A2 (en) Propynylaminothiazole derivatives
US11572337B2 (en) Antibacterial agents: arylalkylcarboxamido phloroglucinols
WO2025144774A1 (en) Therapeutic compounds and methods
WO2023023378A1 (en) DUAL-TARGETED RNA POLYMERASE INHIBITORS: CONJUGATES OF BENZOXAZINO- AND SPIRO-RIFAMYCINS WITH Nα-AROYL- N-ARYL-PHENYLALANINAMIDES
CA3130396A1 (en) Isotopically-stabilized tetronimide compounds

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16873963

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 16873963

Country of ref document: EP

Kind code of ref document: A2