WO2017011983A1 - Uses of mussel adhesive protein in protection of skin and accessory organs of skin - Google Patents

Abstract

Disclosed are applications of a mussel adhesive protein or preparations thereof in protection of skin and accessory organs of the skin. Specifically disclosed are applications of the mussel adhesive protein or preparations thereof in preventing skin, hair and other accessory organs of the skin from suffering from damages caused by ultraviolet rays and the like, applications in protecting skin and accessory organs of the skin from suffering from radiation damages caused by electromagnetism, rays, computer radiation and the like, applications in protecting skin, hair and other accessory organs of the skin from suffering from damages caused by high-temperature objects and the like, applications in protecting skin, hair and other accessory organs of the skin from suffering from damages caused by low-temperature objects and the like, applications in protecting skin, hair and other accessory organs of the skin from suffering from damages caused by seawater and the like, applications in protecting skin, hair and other accessory organs of the skin from suffering from damages caused by environmental pollution, and applications in protecting skin, hair and other accessory organs of the skin from suffering from damages caused by a perming reagent, a hair coloring reagent and the like.

Description

Mussel mucin for the protection of skin and skin accessory organs Technical field

The present invention generally relates to the fields of daily chemical products, cosmetics, medical devices, pharmaceuticals, disinfecting products, health care products, and the like, and relates to the use of mussel mucin or a preparation thereof for protecting skin and skin accessory organs.

Background technique

As the body's first physiological defense line and the largest organ, the skin always participates in the functional activities of the body, maintaining the unity of the body and the natural environment. Any abnormality of the body can also be reflected on the skin surface. The skin consists of the epidermis, dermis, and subcutaneous tissue, and contains many accessory organs such as hair, sweat glands, sebaceous glands, nails, toenails, and blood vessels, lymphatic vessels, nerves, and muscles. The skin has near-perfect physiological protection functions, such as barrier function, sensory function, body temperature regulation, absorption, secretion and excretion, which play an important role in maintaining the health of the body.

The skin and its appendages are susceptible to damage from ultraviolet rays, radiation (such as electromagnetic waves, radiation, computer radiation, etc.), hot objects, frostbite caused by low temperature objects, sea water, environmental pollution, and hot dyeing agents.

Ultraviolet light (UVB) in sunlight can cause redness, swelling, burning, burning, edema, skin desquamation and other damage to the skin and skin accessory organs; causing hair yellowing, splitting, easy to break, dry Inelastic, and can not be repaired once the damage.

Radiation such as ionizing radiation, including radiotherapy, has a certain therapeutic effect on malignant tumors, but there will be some degree of skin reaction, such as: erythema, burning and itching, desquamation, congestion, edema, blisters, exudation, erosion, Ulcer, skin atrophy, telangiectasia, lymphatic drainage disorders, dark brown spots, pigmentation, etc.

Scalding is a problem of skin redness, blisters, peeling, tissue necrosis, ulceration, ulcers, etc. caused by skin contact with objects above body temperature.

Frostbite is a local injury to the skin and skin accessory organs caused by cold and dampness. It is cold, pale, hard, numbness or loss, redness and congestion, heat, itching, burning pain, blisters, edema, severe pain, and tissue necrosis.

The salt in the sea and the chlorine in the swimming pool can damage the scalp and cause inflammatory reactions such as enlarged pores, and the hair will become dry.

The main pollutants in the air are sulfur dioxide, nitrogen oxides, particulate pollutants, and acid rain. Nitrogen-containing pollutants such as sulfur dioxide, nitrous oxide (N ₂ O), nitrogen monoxide (NO), nitrogen dioxide (NO ₂ ), and nitrogen trioxide (N ₂ O ₃ ) are highly irritating to human skin. . Granular pollutants are large in number and complex in composition. They can be carriers of toxic substances or other pollutants, which can easily cause skin inflammation, large pores, red hair follicles, pigmentation and other problems.

Hair dyes have the effect of changing the color of hair, containing toxic and harmful substances such as metals, oxides, p-phenylenediamine, etc., causing scalp allergies, redness, itching and the like. The thioglycolic substances in the perm agent are harmful to the hair, scalp and hair follicles, causing the scales to be damaged, which may cause the hair to become yellow, brittle, detached, tarnished and elastic.

Mussel adhesive protein (MAP), also known as Mytilus edulis foot protein (Mefp), is a marine shellfish, Mytilus edulis Linnaeus, and a small shell mussel (Mytilus coruscus). , A special protein secreted by Perna viridis. Mussels are usually attached in groups to the reefs on the coast or to the bottom of the ship, and have the ability to withstand wave impacts in the offshore. In fact, mussels can be attached extremely strongly to the substrate of any material, such as metal, wood, glass, and the like. The main reason for the above characteristics of mussels is that they can form and store this special mucin in the girth of the foot. The mussels release the mucin through the foot silk to a solid surface such as rock to form a water-resistant combination. Fix yourself.

Currently, 11 mucin subclasses have been identified from mussels, including mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL-P, pre-COL-D. , pre-COL-NG, foot silk matrix proteins PTMP and DTMP (Zhu Xi et al, Advances in Marine Science, 2014, 32(4): 560-568). Mussel mucin has two structural features: (1) containing lysine, which has a high loading of positive charge; (2) containing 3,4 dihydroxyphenylalanine (DOPA, dopa). The cells and tissues of the human body are negatively charged. Mussel mucin plays a protective and therapeutic role by tightly binding cells and tissues through the electrostatic interaction between its own positive charge and the negative charge of cells and tissues. In addition, dopa oxidation produces ortho-dioxins, which can be cross-linked with unoxidized dopa to form a membrane or a network scaffold, which promotes the protein to adhere more closely and firmly to the surface of the human body, thereby protecting. Mussel mucin is a macromolecular protein that is completely degraded in the human body for about 3-10 days. Its ability to attach to cell tissues is excellent, so that mussel mucin can be stabilized locally and continue to function.

Although mussel mucin has the above characteristics, its current application field is very limited. Commercial mussel mucin products are Cell-Tak from BD Biosciences, MAP Trix from Kollodis, Korea, and Hydrogel from Biopolymer, Sweden. These products are either used directly in the form of mussel mucin solution, or are stored as lyophilized powder formulations and dissolved prior to use. Their primary field of application is limited to microscopic cell adhesion and tissue adhesives. Mussel mucin has also been reported for use in the repair of fetal membranes, as a coating against seawater corrosion, and as a drug-loaded stent for the heart.

Summary of the invention

It is an object of the present invention to provide a mussel mucin product.

Mussel mucin used herein refers to Mytilus edulis Linnaeus, Mytilus coruscus or Perna viridis from the Mytilidae bivalve mollusc. 11 subclasses of mussel mucin, currently known as purified from marine mussels: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL- A mixture of one or more of P, pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP. The mussel mucin used herein may have a pH of from 1.0 to 7.0 in an aqueous solution, and particularly may be in the range of from pH 3.0 to 6.5 to make the therapeutic effect better.

The mussel mucin used herein can be obtained by the following preparation method, for example, a method for separating and purifying mussel mucin using mixed adsorption chromatography in Chinese Patent No. ZL200710179491.0, a kind of carboxy using Chinese Patent No. ZL200710179492.5 A method for purifying mussel mucin by methyl ion exchange chromatography, a method for separating and purifying mussel mucin using salting out and dialysis, Chinese Patent No. ZL200910087567.6.

The mussel mucin used herein may be in the form of a solution or a lyophilized powder, in particular, the concentration of mussel mucin in the product may be 0.1-15.0 mg/ml, and when the concentration is too low, the effect of mussel mucin Not large, when the concentration is too high, the cytotoxicity may be caused by the high positive charge, which is not conducive to play a protective role.

The mussel mucin used herein can also be prepared as a liquid agent by combining it with an excipient. Exemplary mussel mucin fluid The body agent is prepared by dissolving or diluting the mother liquor or lyophilized powder of mussel mucin solution to a certain concentration or pH. The solution for dissolving or diluting may be water, physiological saline, phosphate solution, acetate solution, borate. Solution and so on. The pH of mussel mucin in the final product may be pH 1.0-7.0, and in particular, the therapeutic effect may be better in the range of pH 3.0-6.5.

The mussel mucin used herein can also be prepared as a gelling agent in combination with an excipient. An exemplary mussel mucin gel is prepared by mixing a mussel mucin solution or a lyophilized powder with a gel matrix material, which may be selected from the group consisting of cellulose derivatives, carbomers, and seaweeds. Acid salt, tragacanth, gelatin, pectin, carrageenan, gellan gum, starch, xanthan gum, cationic guar gum, agar, non-cellulosic polysaccharide, ethylene polymer, acrylic resin, polyvinyl alcohol or poly One of carboxyvinyl or any combination thereof.

The mussel mucin used herein can also be combined with excipients to prepare a gel. An exemplary mussel mucin gel is prepared by mixing a mussel mucin solution or a lyophilized powder with a gel matrix, which may include cellulose derivatives, glycerin, non-cellulosic polysaccharides, propylene glycol. One or any combination thereof.

The mussel mucin used herein can also be combined with an excipient to prepare a paste. An exemplary mussel mucin paste is prepared by mixing mussel mucin with a paste matrix material, which may include glycerin, petrolatum, paraffin, and the like.

Those skilled in the art can select the above dosage form or other suitable dosage form according to the clinical indications and skin lesion characteristics of different stages.

The mussel mucin used herein can also be combined with a matrix material to form a dressing and application for the skin surface. The liquid in the exemplary mussel mucin application may be a mussel mucin solution or a cellulose derivative, carbomer and alginate, tragacanth, gelatin, pectin, carrageenan, Gellan gum, starch, xanthan gum, cationic guar gum, agar, non-cellulosic polysaccharide, ethylene polymer, acrylic resin, polyvinyl alcohol or carbomer, gelatin, fish gelatin, pectin, alginate, glycerin One or more combinations of petrolatum, paraffin, polyethylene glycol, vitamins, glutathione. The above mussel mucin solution or combination is immersed in a matrix material such as gauze, non-woven fabric, silk paper, and the application may be, for example, a wound patch, a mask, an eye mask, a hand mask, a foot film, or the like.

The mussel mucin used herein can also be made into a foaming agent. An exemplary mussel mucin foaming agent is prepared by mixing a mussel mucin solution or a lyophilized powder with a foaming agent matrix, which may include hydroxypropylmethylcellulose, gelatin, and polyethylene. One or a combination of sodium lauryl sulfate, fatty alcohol sodium polyoxyethylene ether sulfonate, corn gluten powder, acrylamide. It is particularly beneficial that the foaming agent releases pressure during the defoaming process, which is more conducive to the adhesion, penetration and onset of mussel mucin in the affected area. At the same time, the defoaming time of the foaming agent is long, and the action time is prolonged, so that the mussel mucin is more effective.

Further, to the above various preparations including mussel mucin, glycerin, polyethylene glycol, vitamins, glutathione, and the like which are known in the art to increase their moisturizing and anti-oxidation properties can be further added to further enhance moisturization, Antioxidant ability.

Further extracting aloe vera, grape seed, tea, ginseng, snail, etc., amino acid, collagen, hyaluronic acid and the like into various preparations including mussel mucin, and increasing the ability of nurturing skin and skin accessory organs Increase the degree of smoothness.

All of the above preparations can be prepared by methods well known in the art, and detailed procedures can be referred to, for example, "Pharmaceutics".

The mussel mucin used herein can be used as a main raw material to prepare a medicine using a pharmaceutically acceptable carrier. Place The drug may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent. The medicament can be used for in vitro use, in particular for external use on the skin.

The mussel mucin used herein can be used as a main raw material to prepare a medical device. The term medical device as used herein refers to materials that are used directly or indirectly to the human body and other similar or related items. The medical device may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent. The medical device can be used for in vitro use, in particular for external use on the skin.

The mussel mucin used herein can be used as a main raw material to prepare a cosmetic using an auxiliary material which is acceptable in the field of cosmetics. The cosmetic may be a liquid, a gel, a gel, a paste, an applicator, or a foaming agent. The cosmetic can be used for in vitro use, in particular for topical application to the skin.

The mussel mucin used herein can be used as a main raw material to prepare a disinfecting product using an excipient which is acceptable in the field of disinfecting products. The term disinfecting product as used herein refers to a disinfectant, a disinfecting device, a sanitary article, and a disposable medical article that chemically, physically, or biologically kill or eliminate pathogenic microorganisms in the environment. The disinfecting product may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent. The disinfecting product can be used for in vitro use, in particular for external use on the skin.

The mussel mucin used herein can be used as a main raw material, and a health care product or food can be prepared by using an excipient which is acceptable in the field of health care or food. The health care product or food may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent. The health supplement or food can be used for in vitro use or consumption, in particular for external use on the skin.

The mussel mucin used herein can be used as a main raw material to prepare a daily chemical product using an auxiliary material acceptable in the field of daily chemical products. The term daily chemical product as used herein refers to technical chemicals that are used daily, including shampoos, shower gels, and the like. The daily chemical product may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent. The daily chemical product can be used for in vitro use, in particular for external use on the skin.

In particular, hair care and hair care products can be prepared from the mussel mucin of the present invention for protection of scalp and hair and other accessory organs.

Another object of the invention is to provide the use of mussel mucin products in the protection of skin and skin appendages.

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from ultraviolet damage and the like.

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting skin and skin accessory organs from radiation (electromagnetic, radiation, computer radiation, etc.).

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting a skin accessory organ such as skin and hair from a burn caused by a high temperature object or the like.

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting a skin appendage such as skin and hair from frostbite caused by a low temperature object or the like.

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from seawater damage, and for protecting skin accessory organs such as skin and hair from environmental pollution.

In particular, the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from damage such as hot and dyeing agents.

detailed description

Embodiments of the invention include:

1. The application of mussel mucin in the protection of skin and skin accessory organs.

2. The mussel mucin application according to embodiment 1, wherein the mussel mucin may be from a subclass: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen A mixture of one or more of pre-COL-P, pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP.

3. The mussel mucin application according to embodiment 1, wherein the mussel mucin concentration may be from 0.1 to 15.0 mg/ml.

4. The mussel mucin application according to embodiment 1, wherein the mussel mucin is used in the form of a liquid, a gel, a gel, a paste, an applicator or a foaming agent.

5. Mussel mucin application according to embodiment 1, wherein the mussel mucin in the final product may be in the range of pH 1.0-7.0, in particular in the range of pH 3.0-6.5.

The mussel mucin application according to any one of embodiments 1 to 4, wherein the protective skin and skin appendages may include skin appendages such as skin and hair from ultraviolet rays, radiation, high temperature objects, low temperature objects, Damage to seawater, environmental pollution, hot and dyed reagents.

7. Use of mussel mucin as an active ingredient in a composition for protecting skin and skin accessory organs, wherein the composition may be a liquid agent, a gel, a gel, a paste, an applicator or a foam. Use of the agent.

8. The mussel mucin application according to embodiment 7, wherein the composition may be external to the skin.

9. The use of mussel mucin as an active ingredient in medicines for protecting skin and skin accessory organs.

10. Use of mussel mucin as an active ingredient in cosmetics for protecting skin and skin accessory organs.

11. Use of mussel mucin as an active ingredient in a disinfecting product for protecting skin and skin accessory organs.

12. Use of mussel mucin as an active ingredient in health care products or foods for protecting skin and skin accessory organs.

13. Use of mussel mucin as an active ingredient in a daily chemical product for protecting skin and skin accessory organs.

14. Use of mussel mucin as an active ingredient in hair care and hair care products for protecting skin and skin accessory organs.

15. The mussel mucin application according to embodiment 14, wherein the hair care and hair care product can comprise a conditioner, a hair care essence water, a hair care gel, and the like.

The mussel mucin application according to any one of embodiments 7-14, wherein the mussel mucin is used as a foaming agent.

The invention will now be further described in conjunction with specific embodiments. It should be noted that the pharmaceutical, medical device, cosmetic, disinfecting product, health care product or food, and daily chemical product formed by various preparations of mussel mucin or mussel mucin in the present invention are applied to a subject. Both can be applied to the indications described above and exhibit the functions described above, all dosage forms within the scope of the invention have been tested, hereinafter only for illustration, only one of which is described in the examples It is a few, but should not be construed as limiting the invention.

Unless otherwise stated, the reagents used in the present invention are commercially available.

Example 1: Application of mussel mucin hydrogel cosmetics in ultraviolet protection (sun protection).

Take 10g of gelatin, add 100ml of deionized water, warm bath at 90°C for 30min until the matrix is completely dissolved, take another 60ml of mussel mucin solution with a concentration of 20.0mg/ml, add to the gel matrix while stirring, adjust the concentration with physiological saline. After mixing uniformly, a mussel mucin hydrogel cosmetic was formed, wherein the mussel mucin concentration was 10 mg/g.

Ten volunteers were collected, and there was no history of photo-sensing disease. In the near future, no drugs affecting photosensitivity were used. The skin of the test site was free from pigmentation, inflammation, scarring, pigmentation, and hairiness. Using a daylight simulator Xenon arc lamp as a light source, ultraviolet rays having a wavelength of 290 nm to 400 nm are continuously generated, and erythema appears on the skin of the subject from the back to the test site. The above mussel mucin hydrogel cosmetic was weighed and coated at a dose of 2 mg sample/cm ² with a coating area of 40 cm ² to test the lowest erythema irradiation after protection of mussel mucin products and unprotected skin. dose.

The sun protection factor SPF value was calculated using the SPF = minimum erythema dose (after sunscreen) / minimum erythema dose (before sunscreen) formula.

The sun protection factor of the mussel mucin hydrogel cosmetic of this example was measured to be SPF 25. It is indicated that the mussel mucin product has the function of protecting the skin from ultraviolet rays.

Example 2: Application of mussel mucin liquid cosmetics in ultraviolet protection (sun protection).

50 ml of a mussel mucin solution having a concentration of 10.0 mg/ml was taken, diluted with physiological saline, and 10 mg of vitamin C powder was added to prepare a mussel mucin liquid cosmetic, wherein the mussel mucin concentration was 2 mg/ml.

Twelve volunteers were collected, and there was no history of photo-sensing disease. In the near future, no drugs affecting photosensitivity were used. The skin of the test site was free of pigmentation, inflammation, scars, pigmentation, and hairy. Using a daylight simulator Xenon arc lamp as a light source, ultraviolet rays having a wavelength of 290 nm to 400 nm are continuously generated, and erythema appears on the skin of the subject from the back to the test site. Sample Press 2mg / cm ² was weighed amount of mussel adhesive protein hydrogel, the above-described cosmetics and spraying, the minimum erythema spray test area ² over 30-40cm mussel adhesive protein product after discharge protection and protection of the skin without Irradiation dose.

The sun protection factor SPF value was calculated using the SPF = minimum erythema dose (after sunscreen) / minimum erythema dose (before sunscreen) formula.

The sun protection factor of the mussel mucinized liquid cosmetic of the present example was measured to be SPF 15. It is indicated that the mussel mucin product has the function of protecting the skin from ultraviolet rays.

Example 3: Application of mussel mucin gel medical device in radiation therapy protection.

Take 20g of hydroxypropyl methylcellulose, add 100ml of deionized water, warm bath at 90 °C for 30min until the matrix is completely dissolved, add 5g of propylene glycol, 8g of glycerol to prepare the gel matrix, and take the mussel viscosity of 20.0mg/ml. 100 ml of the protein solution, diluted with physiological saline, added to the gel matrix while stirring, and uniformly mixed to form a mussel mucin gel medical device, wherein the mussel mucin concentration was 8 mg/g. Control product preparation: A commercially available carbomer gel medical device that does not contain mussel mucin.

Ten volunteers who were undergoing radiotherapy for lumbar spine were collected. Five patients were coated with the mussel mucin gel medical device half an hour before radiotherapy, and the sample was weighed according to the amount of 5 mg sample/cm ² and then irradiated. Five patients were treated with radiotherapy for control medical devices without mussel mucin. Five patients who were coated with mussel mucin gel medical devices showed no skin damage after radiotherapy. In 5 patients who were coated with carbomer gel, 2 patients developed a degree I burn and showed erythema. It is indicated that the mussel mucin product of the present invention has an effect of protecting the skin from radiation.

Example 4: Application of mussel mucin foam cosmetic in computer radiation protection.

Take 10g of hydroxypropyl methylcellulose, add normal saline, prepare 0.5v/v% foaming agent at room temperature, and stabilize overnight; take another 10ml of mussel mucin solution with concentration of 20.0mg/ml, diluted with physiological saline To 2.5 mg/ml. Fill into a foam spray bottle for use. Control product preparation: A commercially available carboxymethyl cellulose substrate gel cosmetic product, which does not contain mussel mucin.

Collect 20 volunteers between the ages of 20 and 40. Occupational characteristics: The daily use of the cathode ray tube computer screen is not less than 6 hours. Twenty volunteers used the test group mussel mucin foam cosmetic on one side of the cheek, and used the control product on one cheek to select the 1% body surface area of the cheek as the observation area, which was used during, during and after daily contact with the computer. 3 times, continuous use for 90 days.

The melanin value of the observed site was measured before use, and the melanin value of the observed site was measured on the 90th day after use. Using mussel mucin foam to make one side of the cheek, the melanin increased by 5.7% ± 1.2% after 90 days; using the cheek of one side of the control product, the melanin increased by 12.4% ± 0.9% after 90 days. The increase in melanin in the control group was significantly higher than in the experimental group.

It is indicated that the mussel mucin product has the function of protecting the skin from radiation.

Example 5: Application of mussel mucin liquid cosmetics in the protection of environmental pollutants.

100 ml of a mussel mucin solution having a concentration of 5.0 mg/ml was taken, diluted with physiological saline, and 3 mg of glutathione was added to prepare a mussel mucin liquid cosmetic, wherein the mussel mucin concentration was 0.5 mg/ml.

Thirty volunteers, aged between 20 and 40 years old, lived in Beijing and Hebei provinces and had particulate matter contamination (PM2.5 particles). 30 volunteers used the test group mussel mucoid liquid cosmetics on one cheek, and used a commercially available clean lotion as a control group on one cheek, and selected 1% body surface area of the cheek as the observation area, and used each morning and evening after facial cleansing. 2 times, continuous use for 180 days.

The melanin value of the observed site was measured before use, and the melanin value of the observed site was measured on the 180th day after use. Using the mussel mucin liquid cosmetic side of the cheek, the melanin increased by 3.9% ± 2.7% after 180 days; using the cheek of one side of the control product, the melanin increased by 18.4% ± 1.5% after 180 days. The increase in melanin in the control group was significantly higher than in the experimental group.

It is indicated that the mussel mucin product has the function of protecting the skin from environmental pollution.

Example 6: Application of mussel mucin liquid medical device in skin burn protection.

50 ml of a mussel mucin solution having a concentration of 10.0 mg/ml was prepared and diluted with physiological saline to prepare a mussel mucin liquid medical device, wherein the mussel mucin concentration was 2.5 mg/ml.

Take 20 200g white rats, spray the mussel mucin liquid medical device on the right side of the back, once/min, spray the same part 5 times continuously; spray the normal saline on the left part of the back as the control, 1 time/min, the same The part was sprayed 5 times in succession. After anesthesia in rats, the rats were fixed on a rat plate, and the 90 ° C water tank was placed in a 2 cm diameter plastic bottle, and placed continuously in the test site. Set for 30 sec to form a burn.

After 24 hours, cut the burned skin and weigh it, then put it in a 60 °C oven for 48 hours and weigh the dry weight. The skin quality after drying was divided by the skin quality before drying, and the ratio obtained was used to evaluate the degree of edema caused by burns. The higher the ratio, the lighter the edema.

The dry weight/wet weight of the test site of the above-mentioned mussel mucin liquid medical device applied to the right side of 20 rats was 33.4%±3.8% on average; 20 rats were coated with physiological saline on the left side as a control. The dry weight/wet weight of the part was 17.9% ± 2.2% on average.

The edema of the test site coated with the mussel mucin liquid medical device was significantly lighter than that of the control group. It is indicated that mussel mucin products have the effect of protecting the skin from high temperature liquids.

Example 7: Application of mussel mucin hydrogel drug in the protection of skin frostbite.

Take cationic guar 20g, add physiological saline, prepare a hydrogel at room temperature, and stabilize overnight; take another 5ml mussel mucin solution with a concentration of 20.0mg/ml, add to the hydrogel while stirring, gel The final concentration of mussel mucin was 1.8 mg/ml. Control product preparation: a gel containing no mussel mucin as a substrate for cationic guar gum.

Twenty 200 g white rats were used, and the mussel mucin hydrogel drug was applied to the right side of the back, 6 mg/cm ² ; and the control product, 6 mg/cm ² , was applied to the left side of the back. After anesthesia, the rats were fixed on a rat plate, and the -20 ° C water tank was placed in a 2 cm diameter plastic bottle, and the test site was continuously placed for 5 minutes to form a burn.

After 24 hours, cut the burned skin and weigh it, then put it in a 60 °C oven for 48 hours and weigh the dry weight. After drying to the skin quality divided by the quality of the skin before drying, the ratio obtained was used to assess the degree of edema caused by burns. The higher the ratio, the lighter the edema.

The dry weight/wet weight of the test site of the above mussel mucin hydrogel drug coated on the right side of 20 rats was 33.4%±3.8% on average; 20 rats were coated on the left side without mussel mucin The dry weight/wet weight of the test site of the cationic guar gel as a control was 17.9% ± 2.2% on average.

The edema of the test site coated with the mussel mucin product was significantly lighter than that of the control group. It is indicated that mussel mucin products have the effect of protecting the skin from high temperature liquids.

Example 8: Application of mussel mucoid hair gel cosmetic (essential product) in seawater protection.

Take 200ml of mussel mucin solution with a concentration of 3.0mg/ml, dilute with purified water, add 2g of carboxymethylcellulose, 3ml of glycerin, 10ml of aloe extract solution, stir well, and stabilize the preparation for 12h overnight to prepare mussels. The mucin hair care essence, that is, the hair gel cosmetic, wherein the mussel mucin concentration is 1.2 mg/ml.

Twenty volunteers were collected between the ages of 20-30. Ten volunteers in the experimental group took the hair under the microscope and observed the squamous epithelium as the blank control of the experimental group. The above-mentioned mussel mucoprotein hair care essence was evenly sprayed on the surface of the dried hair before swimming, and repeated spraying 3 times under a single area. After completely drying, it was poured into seawater and kept in the water for 3 hours. After the water was taken out, the hair was taken, and the morphology of the scaly skin was observed under a microscope. Ten volunteers in the control group underwent microscopic observation of the squamous epithelium as a control blank, directly into the seawater, and continued in the water for 3 hours. After the water was taken out, the hair was taken and the morphology of the scaly skin was observed under a microscope.

The scaly skin of the test group volunteers using the above mussel mucin hair care essence was intact, and there was no difference from the blank control of the test group. The control group volunteers who did not use mussel mucin hair care essence were incomplete in the scaly rind, which was different from the control blank.

It is indicated that mussel mucin products have the effect of protecting the skin from seawater erosion.

Example 9: Application of mussel mucoprotein hair care liquid cosmetic (essential aquatic product) in allergy protection caused by hair dyeing.

Take 100ml of mussel mucin solution with a concentration of 10.0mg/ml, add 3g of glutathione, and dilute with purified water to prepare mussel mucoprotein hair care essence water, which is a hair care cosmetic. The mussel mucin concentration is 3.9. Mg/ml.

Collect 10 volunteers between the ages of 40-50. Volunteers are sensitive skin and have a history of severe allergies during hair dyeing. Before dyeing the hair, apply the above-mentioned mussel mucin essence water on a dry scalp using a pressure comb that is continuously supplied with hair care essence water, and apply it repeatedly for 3 times until it is completely dry. The above-mentioned mussel mucin hair care essence water is uniformly sprayed on the surface of the hair, and sprayed three times under a single area, and after being completely dried, the hair dyeing starts. After the hair dyeing was completed, it was observed whether there was an allergy phenomenon within 24 hours.

After 24 hours of hair dyeing, 8 of 10 volunteers did not have any allergies, 2 had mild pruritus, and the itching VAS score was about 3 points. It shows that mussel mucoprotein hair care cosmetics have the effect of preventing allergies caused by hair dyeing.

Example 10: Application of Mussel Muscle Hair Care Gel Cosmetic (Essence Product) in allergy protection caused by perm.

Take 50ml of mussel mucin solution with a concentration of 10.0mg/ml, dilute with purified water, and add 10g of gelatin to prepare mussel mucoprotein hair care essence gel, which is a hair care cosmetic. The mussel mucin concentration is 2.0mg/ g.

Six volunteers were collected, aged between 30 and 50 years old. Volunteers are sensitive skin and have a history of severe allergies during perm. Before perming, apply the above mussel mucoid gel on the dried scalp, and when it is completely dry, start perming. After the perm was completed, observe whether there was an allergy phenomenon for 24 hours.

After 24 hours of perming, none of the six volunteers showed any allergies. It shows that mussel mucin products have the effect of preventing allergies caused by perm.

Claims (15)

1. The application of mussel mucin in the protection of skin and skin accessory organs.
2. The mussel mucin application according to claim 1, wherein said mussel mucin is from a subclass: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL a mixture of one or more of -P, pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP.
3. The mussel mucin application according to claim 1, wherein the mussel mucin concentration is from 0.1 to 15.0 mg/ml.
4. A mussel mucin application according to claim 1 wherein said mussel mucin is used as a liquid, gel, gel, paste, dressing or foaming agent.
5. The mussel mucin application according to claim 1, wherein the mussel mucin in the final product is in the range of pH 1.0-7.0, particularly in the range of pH 3.0-6.5.
6. The mussel mucin application according to any one of claims 14 to 4, wherein the protective skin and skin appendages include skin-adhering organs such as skin and hair from ultraviolet rays, radiation, high-temperature objects, low-temperature objects, sea water, environmental pollution, Damage to hot and dyed reagents.
7. Use of mussel mucin as an active ingredient in a composition for protecting skin and skin accessory organs, wherein the composition is used as a liquid, gel, gel, paste, dressing or foaming agent.
8. A mussel mucin application according to claim 7, wherein said composition is for external use on the skin.
9. The use of mussel mucin as an active ingredient in medicines for protecting skin and skin accessory organs.
10. The use of mussel mucin as an active ingredient in cosmetics for protecting skin and skin accessory organs.
11. The use of mussel mucin as an active ingredient in disinfecting products for protecting skin and skin accessory organs.
12. The use of mussel mucin as an active ingredient in health care products or foods for protecting skin and skin accessory organs.
13. The use of mussel mucin as an active ingredient in daily chemical products for protecting skin and skin accessory organs.
14. The use of mussel mucin as an active ingredient in hair care and hair care products for protecting skin and skin accessory organs.
15. The mussel mucin application according to any one of claims 9 to 14, wherein the mussel mucin is used as a foaming agent.