WO2016201257A3 - Compositions and methods for identifying adp-ribosylated sites by mass spectrometry - Google Patents
Compositions and methods for identifying adp-ribosylated sites by mass spectrometry Download PDFInfo
- Publication number
- WO2016201257A3 WO2016201257A3 PCT/US2016/036934 US2016036934W WO2016201257A3 WO 2016201257 A3 WO2016201257 A3 WO 2016201257A3 US 2016036934 W US2016036934 W US 2016036934W WO 2016201257 A3 WO2016201257 A3 WO 2016201257A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mass spectrometry
- adp
- identifying
- methods
- ribosylated
- Prior art date
Links
- 238000004949 mass spectrometry Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 230000004481 post-translational protein modification Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 230000005730 ADP ribosylation Effects 0.000 abstract 1
- 108091005978 ADP-ribosylated proteins Proteins 0.000 abstract 1
- DILVWDXOASDRPN-MROZADKFSA-N [(2r,3r,4r)-3,4,5-trihydroxy-1-oxopentan-2-yl] dihydrogen phosphate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](C=O)OP(O)(O)=O DILVWDXOASDRPN-MROZADKFSA-N 0.000 abstract 1
- 102000006040 nudix hydrolase Human genes 0.000 abstract 1
- 108020003260 nudix hydrolase Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/70—Mechanisms involved in disease identification
- G01N2800/7033—Non-proliferative mechanisms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Urology & Nephrology (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present disclosure relates to the use of a nudix hydrolase(RppH or NudT16) to cleave poly ADP-ribosylated proteins and leave a tag (phosphoribose) to be analyzed by mass spectrometry to identify post-translational modification (PTM) profiles of proteins. The disclosure relates to methods for identifying an ADP- ribosylation profile in hydrolyzed proteins using mass spectrometry.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562173554P | 2015-06-10 | 2015-06-10 | |
US62/173,554 | 2015-06-10 | ||
US201562264834P | 2015-12-08 | 2015-12-08 | |
US62/264,834 | 2015-12-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2016201257A2 WO2016201257A2 (en) | 2016-12-15 |
WO2016201257A3 true WO2016201257A3 (en) | 2017-02-23 |
Family
ID=57504036
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2016/036934 WO2016201257A2 (en) | 2015-06-10 | 2016-06-10 | Compositions and methods for identifying adp-ribosylated sites by mass spectrometry |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2016201257A2 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015073818A1 (en) * | 2013-11-14 | 2015-05-21 | The Brigham And Women's Hospital, Inc. | Parp9 and parp14 as key regulators of macrophage activation |
US10221191B2 (en) | 2015-12-22 | 2019-03-05 | SHY Therapeutics LLC | Compounds for the treatment of cancer and inflammatory disease |
KR102736865B1 (en) | 2017-06-21 | 2024-12-03 | 샤이 테라퓨틱스 엘엘씨 | Compounds that interact with the Ras superfamily for the treatment of cancer, inflammatory diseases, neurodevelopmental diseases and fibrotic diseases |
GB201810697D0 (en) * | 2018-06-29 | 2018-08-15 | Imperial Innovations Ltd | ADP-ribosylation profiling |
US12391705B2 (en) | 2018-12-19 | 2025-08-19 | Shy Therapeutics, Llc | Compounds that interact with the Ras superfamily for the treatment of cancers, inflammatory diseases, rasopathies, and fibrotic disease |
CN110590769B (en) * | 2019-06-13 | 2021-09-24 | 中山大学 | A pair of quinazolinone alkaloid enantiomers and their preparation method and application |
AU2021217816A1 (en) * | 2020-02-03 | 2022-08-11 | Gt Gain Therapeutics Sa | Combination therapy for treating MPS1 |
US20230158028A1 (en) * | 2020-04-06 | 2023-05-25 | The Regents Of The University Of California | Compounds and methods for inducing ucp1 expression |
TW202214574A (en) * | 2020-06-08 | 2022-04-16 | 加拿大商愛彼特生物製藥公司 | Substituted (phthalazin-1-ylmethyl)ureas, substituted n-(phthalazin-1-ylmethyl)amides, and analogues thereof |
WO2024141660A2 (en) * | 2022-12-30 | 2024-07-04 | Aexon Labs. Inc. | Dihydro-quinazoline, -benzothiazine and -benzoxazine derivatives and use thereof as orexin receptors agonists for treating or preventing neurological diseases |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090155834A1 (en) * | 1999-12-15 | 2009-06-18 | Massachusetts Institute Of Technology | Methods for identifying agents which alter histone protein acetylation, decrease aging or increase lifespan |
WO2009077521A1 (en) * | 2007-12-17 | 2009-06-25 | Fondazione Telethon | Method for the isolation and identification of adp-ribosylated molecules |
US20100069372A1 (en) * | 2005-11-07 | 2010-03-18 | The General Hospital Corporation | Compositions and methods for modulating poly(adp-ribose) polymerase activity |
US20130252999A1 (en) * | 2006-12-07 | 2013-09-26 | China Medical University | 2-phenyl-4-quinolones as anticancer agents |
-
2016
- 2016-06-10 WO PCT/US2016/036934 patent/WO2016201257A2/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090155834A1 (en) * | 1999-12-15 | 2009-06-18 | Massachusetts Institute Of Technology | Methods for identifying agents which alter histone protein acetylation, decrease aging or increase lifespan |
US20100069372A1 (en) * | 2005-11-07 | 2010-03-18 | The General Hospital Corporation | Compositions and methods for modulating poly(adp-ribose) polymerase activity |
US20130252999A1 (en) * | 2006-12-07 | 2013-09-26 | China Medical University | 2-phenyl-4-quinolones as anticancer agents |
WO2009077521A1 (en) * | 2007-12-17 | 2009-06-25 | Fondazione Telethon | Method for the isolation and identification of adp-ribosylated molecules |
Non-Patent Citations (4)
Title |
---|
DANIELS ET AL.: "Nudix hydrolases degrade proteinconjugated ADP-ribose", SCIENTIFIC REPORTS, vol. 5, 16 December 2015 (2015-12-16), pages 1 - 12, XP055364610 * |
DOLLE ET AL.: "NAD and ADP-ribose metabolism in mitochondria", FEBS JOURNAL, vol. 280, no. 15, 3 June 2013 (2013-06-03), pages 3530 - 3541, XP055364613 * |
PALAZZO ET AL.: "Processing of protein ADP-ribosylation by Nudix hydrolases", BIOCHEM J., vol. 468, no. 2, 19 March 2015 (2015-03-19), pages 293 - 301, XP055364465 * |
WAHLBERG ET AL.: "Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors", NATURE BIOTECHNOLOGY, vol. 30, no. 3, 19 February 2012 (2012-02-19), pages 283 - 288, XP008163087 * |
Also Published As
Publication number | Publication date |
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WO2016201257A2 (en) | 2016-12-15 |
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