WO2016148545A1 - Composition for preventing or improving male menopause symptoms - Google Patents

Abstract

The present invention provides a composition for preventing or improving male menopause symptoms, the composition comprising a complex extract of carob and Cudrania tricuspidata as an active ingredient. The composition of the present invention is effective in improving various male menopause symptoms, as the invention alleviates reduction in testosterone, which is a cause of male menopause symptoms, and improves capacity for exercise and inhibits the activity of 5α-reductase and thereby exhibits an effect in preventing and treating prostate hypertrophy. The present invention provides a composition for improving male reproductive function, the composition comprising a complex extract of carob and Cudrania tricuspidata as an active ingredient. The composition of the present invention exhibits an outstanding effect in improving male reproductive function by increasing the sperm concentration in seminal fluid, increasing sperm motility and increasing the secretion of testosterone. The complex extract of carob and Cudrania tricuspidata of the present invention exhibits a far better effect in improving male menopause symptoms and effect in improving male reproductive function than single extractions of carob and Cudrania tricuspidata.

Description

Composition for preventing or improving menopausal symptoms

The present invention relates to a composition for preventing or improving menopausal symptoms.

As menopause comes, there is a rapid decrease in female hormones, and men similarly endocrine changes. Male menopause, also known as "late onset hypogonadism" (LOH), male hormone deficiency syndrome, and male climacteric syndrome, is associated with an increase in age in men and is associated with deficiency of serum testosterone and According to the characteristic clinical and biochemical symptoms. Representative symptoms include decreased blood testosterone, impaired erectile energy, decreased libido, passive attitude, depression, muscle weakness, osteoporosis sleep disorder, blood cholesterol metabolic abnormalities, and lowered the quality of life and the function of various organs. It can have a negative effect.

Male hormone replacement therapy has been tried to improve menopausal syndrome, and many studies on the relationship between menopausal symptoms and testosterone have been known, and male menopausal disorder is a new indication of hormone therapy using testosterone. Attention is on the rise.

Testosterone supplementation has been applied or studied in diabetes mellitus, bone metabolism, body fat and muscle strength, cardiovascular, sexual and mood effects, and cognitive effects associated with male menopause, but incomplete, especially prostate by testosterone supplementation Side effects such as hypertrophy, prostate cancer, and heart disease have been reported and have limitations. Therefore, there is a need to develop improvers and treatments that can be used safely mentally and physically.

Throughout this specification, many papers and patent documents are referenced and their citations are indicated. The disclosures of cited papers and patent documents are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors have made intensive research efforts to develop male menopausal symptoms and male reproductive function effectively and to develop substances that are safe for human body, especially plant-derived substances. As a result, the present invention was completed by experimentally clarifying that the combination of carob and kkujippong extract has a significantly increased male menopausal symptom improvement effect and a male reproductive function improvement effect compared to a single extract of carob or kkujippong.

Therefore, it is an object of the present invention to provide a food composition for preventing or improving menopausal symptoms of men and women including a complex extract of carops and kkujippong as an active ingredient.

Another object of the present invention to provide a food composition for improving male reproductive function comprising a complex extract of carob and kkujippong as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to one aspect of the invention, the present invention provides a food composition for preventing or ameliorating male menopausal symptoms comprising a complex extract of carob and kkujippong as an active ingredient.

The present inventors have made diligent efforts to develop male menopausal symptoms and male reproductive function effectively, and to develop substances that are safe for the human body, especially plant-derived substances. In comparison, it was experimentally found that the male menopausal symptoms and the male reproductive function were improved significantly.

According to the present invention, the combination extract of Carp and kkujippong of the present invention alleviates the decrease in testosterone, which is the cause of menopausal symptoms, improves exercise ability, and inhibits the activity of 5-α reductase to prevent and treat prostatic hyperplasia. It is effective in improving various male menopausal symptoms by showing effects.

The method for separating the carob and kkujippong extracts, which are the active ingredients in the composition of the present invention, is carried out according to conventional methods known in the art for extracting extracts from natural products, that is, using conventional solvents under the conditions of conventional temperature and pressure. Can be separated.

Various extractive solvents may be used in the case where the extract of carob and kkujippong used in the composition of the present invention is obtained by treating the extract with carob or jacuzzi. According to one embodiment of the present invention, a polar solvent or a nonpolar solvent may be used. Suitable polar solvents include (i) water, (ii) alcohols (preferably methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol Or ethylene glycol), (iii) acetic acid, (iv) dimethyl-formamide (DMFO) and (v) dimethyl sulfoxide (DMSO). Suitable as nonpolar solvents are acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1- Pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloro Methane, 1,2-dichloroethane, anneal, diethylamine, ether, carbon tetrachloride and THF.

According to another embodiment of the present invention, the extraction solvent used in the present invention is (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) the Mixed solvent of lower alcohol with water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) butyl acetate, (h) 1,3-butylene glycol, (i) hexane and (j) Diethyl ether. According to a particular embodiment of the present invention, the extract of the present invention is obtained by treating hot water with carob or jacuzzi.

As used herein, the term "extract" has the meaning commonly used as a crude extract in the art as described above, but also broadly includes a fraction additionally fractionating the extract. That is, the carob or kkujippong extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the carob or kkujippong extract of the present invention.

Carob and kkujippong extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

In the present specification, the term 'comprising as an active ingredient' means to include an amount sufficient to achieve the efficacy or activity of the following carp and kkujippong. The present invention is a composition extracted from the natural plant material, Carob and kkujippong, so even if excessive administration to the human body has no side effects, the upper limit of the quantity contained in the composition of the Carp and kkujippong extract of the present invention can be carried out by those skilled in the art selected within the appropriate range. .

Male menopausal symptoms to which the composition of the present invention can be applied include pain in the joints or muscles, osteoporosis, enlarged prostate, hyperhidrosis, sleep disorders, excitement, nervousness, emotional anxiety, lethargy, fatigue, muscle strength, weakness, vitality, facial Redness, sleep disorders, depression, decreased energy, lowered libido, lowered erection, decreased energy, decreased motor performance, reduced body hair, aging or decreased urogenital function.

According to one embodiment of the present invention, Carob and kkujippong composite extract of the present invention is prepared by mixing the Carob and kkujippong extract in a 9: 1 to 6: 4 weight ratio.

Preferably, the mixing ratio of the complex extract is 9: 1 to 6: 4, more preferably, the composition ratio of the mixture is 8: 2 to 6: 4, and most preferably, the mixing ratio of the complex extract is 7 : 3.

The composition of the present invention may be provided as a food composition. When the composition for the prevention or improvement of male menopausal symptoms including the complex extract of carotene and kkujippong of the present invention as an active ingredient is prepared as a food composition, as well as caropy and kkujippong as an active ingredient, it is commonly added during food preparation. Ingredients, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. Examples of the above carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agents [tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared with a drink, in addition to the white rice extract of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, tofu extract, jujube extract, licorice extract, and the like may be further included. have.

According to another aspect of the present invention, the present invention provides a food composition for improving male reproductive function comprising a complex extract of carob and kkujippong as an active ingredient.

Carob and kkujippong complex extract of the present invention is effective in improving male reproductive function by increasing sperm concentration in semen, increase sperm motility, and increase the secretion of testosterone.

The composition of the present invention regulates the secretion of testosterone, a hormone associated with the sperm count and the mechanism of sperm motility in men, thereby restoring male sexual function and enhancing vitality.

The features and advantages of the present invention are summarized as follows:

The present invention provides a composition for the prevention or improvement of male menopausal symptoms comprising a complex extract of carob and kkujippong as an active ingredient.

The composition of the present invention alleviates the decrease in testosterone, which is the cause of male menopausal symptoms, improves exercise ability, and inhibits the activity of 5-α reductase, thereby preventing and treating various menopausal symptoms by prostatic hypertrophy. Effective for improvement.

The present invention provides a composition for improving male reproductive function comprising a complex extract of carob and kkujippong as an active ingredient.

The compositions of the present invention have an excellent effect on improving male reproductive function by increasing sperm concentration in semen, increasing sperm motility, and increasing the secretion of testosterone.

The composite extract of carob and kkujippong of the present invention shows a markedly increased male menopausal symptom improvement effect and male reproductive function improvement effect as compared to the single extract of carob and kkujippong.

Figure 1 shows the results of measuring the effect on the cell viability of the mixture of the carob extract and kkujippong extract on testicular Leidich cells. 'HCG' refers to a human chorionic gonadotropin, 'Control' is a control, 'CS' is a carob extract, 'CT' is a kkujippong extract, and 'Mix' is a complex of carob extract and kkujippong extract.

Figure 2 shows the results of measuring the effect on the testosterone production of the mixture of the Carob extract and kkujippong extract in testicular Leidihi cells. 'HCG' refers to a human chorionic gonadotropin, 'Control' is a control, 'CS' is a carob extract, 'CT' is a kkujippong extract, and 'Mix' is a complex of carob extract and kkujippong extract.

Figure 3 shows the results of measuring the effect of the mixture of the Carob extract and Cudrania extract on 5-alpha reductase activity in the male prostate tissue. 'Control' refers to the control, 'CS' refers to the carob extract, 'CT' refers to the kkujippong extract, and ‘Mix’ refers to the complex of carob and kkujippong extract.

Figure 4 shows the results of measuring the effect of the mixture of carob extract and kkujippong extract on the exercise ability of male rats. 'Control' refers to the control, 'CS' refers to the carob extract, 'CT' refers to the kkujippong extract, and ‘Mix’ refers to the complex of carob and kkujippong extract.

Figure 5 shows the results of measuring the effect of the mixture of Carob extract and kkujippong extract on the sperm number of male rats. 'Control' refers to the control, 'CS' refers to the carob extract, 'CT' refers to the kkujippong extract, and ‘Mix’ refers to the complex of carob and kkujippong extract.

Figure 7 shows the results of measuring the effect of the mixture of carob extract and kkujippong extract on the sperm water improvement rate of male rats. 'Control' refers to the control, 'CS' refers to the carob extract, 'CT' refers to the kkujippong extract, and ‘Mix’ refers to the complex of carob and kkujippong extract.

Figure 7 shows the results of measuring the effect of the mixture of Carob extract and kkujippong extract on the sperm motility of male rats. 'Control' refers to the control, 'CS' refers to the carob extract, 'CT' refers to the kkujippong extract, and ‘Mix’ refers to the complex of carob and kkujippong extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Preparation of Carob Extract or Cudrania Extract and Preparation of Mixture

Carob ( Ceratonia siliqua ) Preparation of Extract

After selecting and pulverizing the carob, the extract is extracted by hot water extraction for about 4 hours using about 6 times the volume (w / v) of purified water as a solvent, followed by secondary extraction by adding 4 times purified water. . The extract was directly filtered, concentrated in vacuo, mixed with dextrin in a 1: 1 ratio, and spray dried or lyophilized to obtain a carob extract.

Cudrania ( Cudrania tricuspidata ) Preparation of Extract

After crushing and crushing kkujippong, using about 6 times the volume (w / v) of purified water of purified water as a solvent and extracted by hot water extraction method for about 5 hours, 5 times purified water is added to the second extraction. The extract was immediately filtered and concentrated in vacuo, mixed with dextrin in a 1: 1 ratio, and spray dried or lyophilized to obtain a zigzag extract.

Carob  Extract and Cudrania  Preparation of mixtures of extracts

Carob extract and kkujippong extract obtained from the above embodiment is mixed by 10: 1 to 1:10, preferably 9: 1 to 8: 2, more preferably 7: 3 to 6: 4 ratio and Prepare a mixture of Cudrania extract.

Test Example 1 Testosterone Increasing Effect

Test Example  1-1: Leidihi Cell  Incubation and Treatment of Extracts

Leydig cells, mouse TM3 cells, were cultured in DMEM medium with 10% FBS, penicillin (100 μg / ml) and streptomycin (100 μg / ml) at 37 ° C. and 5% CO ₂ . After changing to DMEM medium containing 1% FBS, carob extract, courageous extract, and a mixture of carob extract and courageous extract were treated, respectively. In addition, hCG (human chorionic gonadotropin, 6 mIU / 200 μl medium; Sigma-Aldrich, USA) was further incubated for 24 hours to induce testosterone production and secretion.

Test Example  1-2: Cell viability measurement

TM3 cells were seeded in 96-well (Nunc, Slangerup, Denmark) at a density of 15,000 cells / well and incubated for 24 hours. The cell culture was changed to DMEM containing 1% FBS and further incubated for 2 hours. To 50 μl of the Carob extract in cultured cells; Kkujippong extract to 50 μl concentration; Or add a mixture of carob extract and kkujippong extract to 50 μ concentration and the same amount of PBS (pH 7.4) as a negative control group, and then add hCG (6 mIU / 200 μ medium) of Test Example 1-1 24 hours were further incubated. After 10 μ treatment of alamarblue (Serotec, Oxford, UK) and incubated for 3 hours, the cell viability was analyzed by measuring the absorbance at 570 nm using an ELISA Reader (Molecular Divices, Sunnycale, CA, USA). The background absorbance (blank count) was used as measured at 600 nm. Cell viability was calculated using the following formula.

The results are expressed as mean and standard error, and statistically, Student's t-test was used for statistical significance. The results are shown in FIG. 1.

As a result of the experiment, as shown in Figure 1, a slight increase in cell viability compared to the control (Control) due to the treatment of the carob extract (CS), kkujippong extract (CT), and the complex (Mix) of the carob extract and kkujippong extract Although there was no statistically significant change. Therefore, it was confirmed that the composition of the present invention does not affect the cell viability.

Test Example  1-3: Testosterone production measurement

After 24 hours of treatment with the extract and hCG by the method of Test Example 1-1, using the ELISA kit (DRG Instruments, Marburg, Germany), the testosterone production was measured according to the manufacturer's instructions. Testosterone amount was calculated by dividing the amount of protein in each sample, and after three repeated measurements, averaged to obtain the result.

The results were expressed as mean and standard error. For statistical significance test, Student's t-test was used and it was considered as significant at p <0.05. The results are shown in FIG.

As a result of the measurement, as can be seen in Figure 2, it can be confirmed that the testosterone production in the Leidihi cells increased by hCG treatment. In the case of carob extract (CS) and kkujippong extract (CT), respectively, testosterone production increased by about 25% and 23%, respectively, compared to the control group treated with PBS. And when treated with the complex (Mix) it was confirmed that the testosterone production increased more than this increased by more than 55% compared to the control. This confirmed that the complex further increased testosterone production of Leidihi cells compared to a single extract.

Test Example 2: Improvement of Prostatic Hyperplasia

5-alpha Reductase  Active reduction effect

Benign prostatic hypertrophy is a menopausal symptom seen in middle-aged men after their 40s. In patients with prostatic hyperplasia, testosterone is known to have high blood levels of dihydrotestosterone (DHT) converted by 5-alpha reductase. Therefore, by confirming the inhibition rate of 5-alpha reductase activity of the present composition to determine the prophylactic effect of prostatic hyperplasia, one of the menopausal symptoms.

Prostate glands were extracted from male rats of the Sparague-Dawley line of about 400-450 g body weight, washed once with physiological saline and homogenized with PBS. The homogeneous solution was centrifuged at 4 ° C. and 5,000 rpm for 5 minutes, and only the supernatant was used for the experiment. 5-alpha reductase II activity was measured as described using the rat 5-α reductase II ELISA kit (Cusabio). 200 μg of homogenate and carob extract, kkujippong extract, complex of carob extract and kkujippong extract were reacted at room temperature for 30 minutes, treated with testosterone 2 nM at 37 ° C. for 1 hour, and then absorbance at 450 nm wavelength. Measured. The results were expressed in percentage with 100% of testosterone alone, expressed as mean and standard error, and statistically used Student's t-test for statistical significance, and considered as significant at p <0.05. It was. The results are shown in FIG.

As can be seen in Figure 3, it can be seen that the 5-alpha reductase activity in the carob extract (CS) and kkujippong extract (CT) treated group compared to the control group treated only testosterone (Control). In the mixed (Mix) treatment group, the enzyme activity was further reduced, showing only about 10% of 5-alpha reductase activity compared to the control group. As a result, carob extract and kkujippong extract inhibit the activity of 5-alpha reductase, an action enzyme that converts testosterone to DHT.In particular, the complex has a higher inhibitory rate of enzyme activity than a single extract. It was confirmed that it can act as a prophylactic and therapeutic agent of.

Test Example  3: Increased athletic ability  effect

Rat exercise ability increase effect

Male rats (Sampaco Bio Korea, Korea) of Sparague-Dawley strains weighing around 400-450 g, respectively, control group (CTL), carob extract (CS), and cuzipan extract (CT) And 5 rats, each of which was divided into an experimental group in which a mixture of Carob extract and Cudrania extract (Mix) was ingested. During the breeding period, the rats were allowed to freely eat the solid feed and distilled water, and after one week of adaptation, they were dissolved in physiological saline at the same time every morning, or kkujippong extract, or a combination of carops extract and kkujippong extract (7: 3). (Mixture ratio of)) was orally administered at a concentration of 50 mg / kg, respectively, and the control group was orally administered the same amount of saline solution for 1 month. Rat athletic performance was measured using the Rotarod test. The rotarod test is a test that measures the amount of time a rat has run on a cylinder until it falls by running on a rotating cylinder. The rotational speed of the cylinder was maintained at 6 rpm for the first 30 seconds and then accelerated to 40 rpm over 5 minutes. The rotarod test was carried out before the initial administration and after the end of 1 month of administration, and the measured value was averaged by measuring three times the latency time on the cylinder.

The results were expressed as mean and standard error. For statistical significance, statistical analysis was performed using Student's t-test, which was considered significant at p <0.05. The results are shown in FIG.

As a result, as shown in Figure 4, when the carob extract (CS) and kkujippong extract (CT) ingested, respectively, the exercise capacity measured by the rotarod test method was increased by 19% and about 25%, respectively, compared to before intake statistically Significantly improved. In addition, when the compound was ingested, the latency time of rotarod was increased by more than 40% compared to before ingestion, which showed a statistically significant improvement in exercise ability. It was confirmed to further improve.

Test Example 4: Effect of increasing sperm count and sperm motility

Rat Sperm count  And sperm motility effect

Sperm number and sperm motility were measured by extracting the left and right epididymis twice from the experimental animal of Test Example 1 before the start of the extract administration and after the end of the extract administration for 1 month. Respiratory anesthesia was performed with 4% isoflurane, and the left epididymis was extracted, chopped and diluted with PBS before starting administration, and sperm count was measured under an optical microscope with a cell counter. After epididymal extraction, the extraction site was closed, and after 1 month of administration, the right epididymis was taken out in the same manner to measure sperm count.

Sperm motility was incubated for 5 minutes in M199 medium (GIBCO, Thermo Fisher Scientific Inc., MA, USA) containing 0.5% BSA after sperm motility collected by the above method, divided by the total sperm count percentage Converted to.

The results were expressed as mean and standard error. For statistical significance, statistical analysis was performed using Student's t-test, which was considered significant at p <0.05 (* p <0.05). The sperm count and sperm count improvement rate before and after the extract administration are shown in FIGS. 4 and 5, and the sperm motility before and after the extract administration is shown in FIG. 4.

As can be seen in Figures 5 and 6, when the carob extract (CS) and kkujippong extract (CT) respectively ingested and the intake of the complex (Mix), the number of sperm significantly increased compared to before ingestion. In contrast, the control group (CTL) did not significantly change sperm count compared to before ingestion. The sperm count was increased by about 11% and 10% when carotene extract (CS) and kkujippong extract (CT) alone were used, whereas sperm count was improved by more than 30% in the mixed group (Mix). In comparison with the intake of each of the extracts, it was confirmed that the effect of improving sperm count when ingesting the complex.

In addition, as shown in Figure 7, sperm motility measurement results, the control group before and after ingestion of sperm motility was not statistically significant, while in the carob extract (CS) or kkujippong extract (CT) administration group compared to before the administration each about 19 The sperm motility effect was increased by 15% and 15%, and the group (Mix) group showed more than 40% sperm motility improvement compared to before administration. there was.

Test Example 5: Preventing Menopausal Men through Clinical Trials

The male was ingested with the composition of the present invention to determine the effect of preventing and treating male menopausal symptoms through a male menopausal evaluation questionnaire before and after ingestion.

Study subject and period

Forty adults aged 40 years or older who agreed to this test were placed in a control group (placebo) who received placebo and an experimental group who consumed the complex of Carob and Cudrania extracts of the present invention (Carob: Cudrania = 7: 3). They were randomized to receive 400 mg of placebo or experimental drug daily for four weeks. Male menopausal evaluation due to ingestion of the composition was performed by answering the test sheet in Table 1 before and after ingestion.

AMS (Aging) Male ’s  Symptoms)

AMS is a test tool that Heinemann et al. Designed to objectively evaluate the effects of aging on men's quality of life. Its validity and reliability have been proved as an evaluation method. Heinemann LAJ, Zimmermann T, Vermeulen A, Thiel C. A New 'Aging Male's Symptom' (AMS) Scale.The Aging Male 1992, 2: 105-114.). In the Korean version, the validity of Kim Se-hyun's translation has been proved to be reliable (Daig I, Heinemann LA, Kim S, Leungwattanakij S, Badia X, Myon E, et al.e Aging Males' Symptoms (AMS) scale: review of its methodological characteristics Health Qual Life Outcomes 2003; 1: 77.). The Korean version of the AMS questionnaire consists of 17 questions including 5 mental symptoms, 7 physical symptoms, and 5 sexual symptoms (Table 1). All items were quantified as no symptoms (1 point), light (2 points), moderate (3 points), severe (4 points), and very severe (5 points), and the higher the score, the higher the symptom. Representative male menopausal periods such as nervousness, emotional anxiety, depression, hot flashes, sleep disorders, vitality, decreased work ability, decreased libido, impotence, decreased energy, decreased physical strength, reduced exercise ability, and reduced body hair. Symptoms can be assessed.

Symptoms of the body Severity of symptoms none lightness usually Severe Very severe 1. Feeling poorly ill (subjective feeling of overall health) 2. Pain in joints and muscles (sick back, joints, back pain or limb pain) 3. I sweat too much (potatoes are cold even if I don't feel cold) 4. Have trouble sleeping (difficult to sleep, wake up often, sleep early, get tired, can't sleep deep, or can't sleep) 5. I have more sleep but still tired. 6. Excited well (being angry, easily angry, or capricious) 7. My nerves are sensitive (tense, upset or nervous) 8. I'm always anxious. 9. Physically exhausted and lacking energy (a feeling of being forced to do something because of poor performance, lack of activity, indifference to leisure activities, inability to meet targets, or lack of energy, ie For example, when I am sick, I feel like I am forced to go to work.) 10. My strength is falling (I feel weak) 11. I feel depressed (feels calm, sad, tearful, motivated, feelings change, or useless) 12. I feel like my prime is gone. 13. Feeling exhausted emotionally (feeling negative, helpless and hopeless with physical and mental exhaustion) 14. The beard grows slowly. 15. Less energetic and less sexual life. 16. The number of erections in the morning is decreasing 17. Less libido and less sexual impulse (no pleasure in sex life, no desire to live sex life)

The AMS results of the control group and the experimental group are categorized by symptoms of male menopausal symptoms, and the score change according to the symptoms in the control group and the experimental group is shown in Table 2. Results were listed as mean and standard deviation. For the significance test, statistical analysis was performed using Student's t-test, which was considered significant at p <0.05 (* p <0.05).

division Symptom (AMS Item Number) Score change (mean ± standard deviation) Control Experimental group Mental Symptoms (5 questions) Nervousness (6, 7) 0.06 ± 0.12 -1.99 ± 1.08 * Emotional Anxiety (8) 0.10 ± 1.41 -0.70 ± 1.05 * Depression (11, 13) -0.30 ± 1.32 -2.47 ± 1.95 * Physical symptoms (7 questions) Hot flashes (3) 0.31 ± 1.27 -0.73 ± 0.95 * Sleep disorders (4, 5) 0.29 ± 1.05 -1.99 ± 0.92 * Decreased vitality, reduced work capacity (9) 0.34 ± 0.94 -0.81 ± 0.48 * Decrease in fitness, loss of motor skills (1, 2, 10) 0.31 ± 1.60 -2.92 ± 1.28 * Sexual symptoms (5 questions) Decreased libido, erectile dysfunction, decreased energy (12, 15, 16, 17) 0.27 ± 2.08 -4.51 ± 2.38 * Hair Loss (14) 0.60 ± 1.58 -1.04 ± 1.05 * Total points 1.98 ± 4.25 -16.82 ± 6.02 *

As a result, the control group showed no significant change in the male menopausal symptom score evaluated by the AMS test paper before and after the placebo intake. Significantly reduced mental symptoms (nervous nervousness, emotional anxiety, depression), physical symptoms (hot flashes, sleep disorders, vigor, decreased work ability, physical weakness, reduced motor skills), sexual symptoms (sexual desire) Deterioration, erectile dysfunction, energy loss, hair loss) and the like can be seen to be effective.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

The composition for the prevention or improvement of male menopausal symptoms according to the present invention can be used in a wide range of fields, such as food, medicine for preventing or improving male menopausal symptoms.

Claims (10)

Carob ( Ceratonia siliqua and Cudrania tricuspidata ) Food composition for the prevention or improvement of male menopausal symptoms comprising a complex extract of. The method of claim 1, The composite extract is a composition, characterized in that extracted with water, methanol, ethanol or a combination thereof as an extraction solvent. The method of claim 1, The male menopausal symptoms include joint or muscle pain, osteoporosis, prostate hyperplasia, hyperhidrosis, sleep disorders, excitement, nerve hypersensitivity, emotional anxiety, lethargy, fatigue, muscle weakness, weakness, vitality, hot flashes, sleep disorders, depression, A composition characterized in that the lowered energy, lowered libido, lowered erection, reduced energy, reduced athletic performance, reduced body hair, aging or lower urogenital function. The method of claim 1, The composite extract composition, characterized in that to increase the production of testosterone. The method of claim 1, The composite extract composition, characterized in that to increase the exercise ability. The method of claim 1, The complex extract is a composition, characterized in that to inhibit the activity of 5-α reductase (reductase). The method of claim 1, The complex extract is a composition characterized in that the carob extract and kkujippong extract in a 9: 1 to 6: 4 weight ratio. Carob ( Ceratonia siliqua and Cudrania tricuspidata ) Food composition for improving male reproductive function comprising a complex extract of. The method of claim 8, The composite extract composition, characterized in that to increase the sperm concentration in semen. The method of claim 8, The composite extract composition, characterized in that to increase sperm motility.

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