WO2016133961A1

WO2016133961A1 - Benzofurans substituted with bicyclic secondary benzamide as hcv inhibitors

Info

Publication number: WO2016133961A1
Application number: PCT/US2016/018168
Authority: WO
Inventors: Tao Wang; Zhiwei Yin; John F. Kadow
Original assignee: Bristol Myers Squibb Co
Current assignee: Bristol Myers Squibb Co
Priority date: 2015-02-19
Filing date: 2016-02-17
Publication date: 2016-08-25
Anticipated expiration: 2017-08-19
Also published as: US20180030056A1; US10125137B2

Abstract

Compounds of Formula I, including their salts, as well as compositions and methods of using the compounds are set forth. The compounds have activity against hepatitis C virus (HCV) and areuseful in treating those infected with HCV: Formule (I)

Description

BENZOFURANS SUBSTITUTED WITH BICYCLIC SECONDARY BENZAMIDE

AS HCV INHIBITORS

CROSS REFERENCE TO RELATED APPLICATION

This application claims the benefit of U.S. Provisional Application Serial No. 62/118,060 filed February 19, 2015 which is herein incorporated by reference in its entirety.

FIELD OF THE INVENTION

The invention relates to novel compounds, including their salts, which have activity against hepatitis C virus (HCV) and which are useful in treating those infected with HCV. The invention also relates to compositions and methods of making and using these compounds.

BACKGROUND OF THE INVENTION

Hepatitis C virus (HCV) is a major human pathogen, infecting an estimated 170 million persons worldwide - roughly five times the number infected by human immunodeficiency virus type 1. A substantial fraction of these HCV infected individuals develop serious progressive liver disease, including cirrhosis and hepatocellular carcinoma (Lauer, G. M; Walker, B. D. N. Engl. J. Med. 2001, 345, 41-52).

HCV is a positive-stranded RNA virus. Based on a comparison of the deduced amino acid sequence and the extensive similarity in the 5 '-untranslated region, HCV has been classified as a separate genus in the Flaviviridae family. All members of the Flaviviridae family have enveloped virions that contain a positive stranded RNA genome encoding all known virus-specific proteins via translation of a single, uninterrupted, open reading frame.

Considerable heterogeneity is found within the nucleotide and encoded amino acid sequence throughout the HCV genome. At least six major genotypes have been characterized, and more than 50 subtypes have been described. The major genotypes of HCV differ in their distribution worldwide, and the clinical significance of the genetic heterogeneity of HCV remains elusive despite numerous studies of the possible effect of genotypes on pathogenesis and therapy.

The single strand HCV RNA genome is approximately 9500 nucleotides in length and has a single open reading frame (ORF) encoding a single large polyprotein of about 3000 amino acids. In infected cells, this polyprotein is cleaved at multiple sites by cellular and viral proteases to produce the structural and non-structural (NS) proteins. In the case of HCV, the generation of mature non-structural proteins (NS2, NS3, NS4A, NS4B, NS5A, and NS5B) is effected by two viral proteases. The first one is believed to be a metalloprotease and cleaves at the NS2-NS3 junction; the second one is a serine protease contained within the N-terminal region of NS3 (also referred to as NS3 protease) and mediates all the subsequent cleavages downstream of NS3, both in cis, at the NS3- NS4A cleavage site, and in trans, for the remaining NS4A-NS4B, NS4B-NS5A, NS5A- NS5B sites. The NS4A protein appears to serve multiple functions, acting as a cofactor for the NS3 protease and possibly assisting in the membrane localization of NS3 and other viral replicase components. The complex formation of the NS3 protein with NS4A seems necessary to the processing events, enhancing the proteolytic efficiency at all of the sites. The NS3 protein also exhibits nucleoside triphosphatase and RNA helicase activities. NS5B (also referred to as HCV polymerase) is a RNA-dependent RNA polymerase that is involved in the replication of HCV. The HCV NS5B protein is described in "Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides (Bressanelli; S. et al, Journal of Virology 2002, 3482-3492; and Defrancesco and Rice, Clinics in Liver Disease 2003, 7, 211-242. Currently, the most effective HCV therapy employs a combination of alpha- interferon and ribavirin, leading to sustained efficacy in 40% of patients (Poynard, T. et al. Lancet 1998, 352, 1426-1432). Recent clinical results demonstrate that pegylated alpha-interferon is superior to unmodified alpha-interferon as monotherapy (Zeuzem, S. et al. N. Engl. J. Med. 2000, 343, 1666-1672). However, even with experimental therapeutic regimens involving combinations of pegylated alpha-interferon and ribavirin, a substantial fraction of patients do not have a sustained reduction in viral load. Thus, there is a clear and important need to develop effective therapeutics for treatment of HCV infection. HCV-796, an HCV NS5B inhibitor, has shown an ability to reduce HCV RNA levels in patients. The viral RNA levels decreased transiently and then rebounded during dosing when treatment was with the compound as a single agent but levels dropped more robustly when combined with the standard of care which is a form of interferon and ribavirin. The development of this compound was suspended due to hepatic toxicity observed during extended dosing of the combination regimens. US patent 7,265,152 and the corresponding PCT patent application WO2004/041201 describe compounds of the HCV-796 class. Other compounds have been disclosed; see for example,

WO2009/101022, as well as WO 2012/058125.

What is therefore needed in the art are additional compounds which are novel and effective against hepatitis C. Additionally, these compounds should provide advantages for pharmaceutical uses, for example, with regard to one or more of their mechanism of action, binding, inhibition efficacy, target selectivity, solubility, safety profiles, or bioavailability. Also needed are new formulations and methods of treatment which utilize these compounds.

SUMMARY OF THE INVENTION

One aspect of the invention is a compound of Formula I, including

pharmaceutically acceptable salts thereof:

I

wherein R¹, R² are each independently selected from the group of hydrogen, halo, nitro, alkyl, cycloalkyl, haloalkyl, aminoalkyl, hydroxyalkyl, alkoxyalkyl, hydroxyl, alkoxy, OR^17, cycloalkoxy, amino, alkylamino, dialkylamino, alkylcarboxamido, alkoxycarboxamido, alkoxyalkylcarboxamido and Ar¹; R³ is selected from the group of cyano, alkoxycarbonyl, (cycloalkyl)oxycarbonyl, (alkylsulfonyl)aminocarbonyl, CONRⁿR¹², (Rⁿ)(R¹²)NCONH, triazolyl, thiazolyl, and tetrazolyl;

R⁴ is phenyl substituted with 0-2 halo substituents;

R⁵ is selected from the group of hydrogen, alkyl, alkylsulfonyl, alkylcarbonyl, alkenyl, alkynyl, haloalkyl, haloalkenyl, haloalkynyl, cyanoalkyl, cyanoalkenyl, cyanoalkynyl, cycloalkyl, halocycloalkyl, (haloalkyl)cycloalkyl,

(halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl,

(cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl,

(hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and

(alkoxycycloalkyl)cycloalkyl;

R⁶ is selected from the group of hydrogen, alkyl, hydroxy alkyl, alkoxy alkyl, alkylsulfonyl, alkenyl, alkynyl, haloalkyl, haloalkenyl, haloalkynyl, cyanoalkyl, cyanoalkenyl, cyanoalkynyl, cycloalkyl, halocycloalkyl, (haloalkyl)cycloalkyl,

(halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl,

(cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl,

(hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and

(alkoxy cycloalkyl)cycloalkyl;

R⁷R⁸N taken together is a fused bicyclic ring, spiro bicyclic ring or a bridged bi cyclic ring constructed from carbon and from 1 - 4 atoms selected from nitrogen, sulfur and oxygen atoms, wherein said ring is substituted with 0-5 substituents selected from the group of alkyl, hydroxyalkyl, alkoxyalkyl, hydroxy, alkoxy, carboxy, alkoxycarbonyl, dialkylcarboxamido, alkylcarbonylamino, alkoxycarbonylamino, halo, haloalkyl, cyano, cyanoalkyl, cyanoalkenyl, cyanocycloalkyl, cyanocycloalkenyl, halocycloalkyl, haloalkenyl, halocycloalkenyl, cycloalkyl, alkenyl, alkynyl, ester, amide, acid, amine, alcohol, ether, cycloether, cyclic amine, lactame, lactone, cycloalkenyl, sulfonyl, aminosulfonyl, amidinyl, guanidinyl, carbamide, urea, and Ar²;

R¹⁷ is selected from the group of haloalkyl, cyanoalkyl, (cycloalkyl)alkyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, (R¹⁸)alkyl, (Ar⁴)alkyl, alkynyl, and

aminocycloalkyl;

R¹⁸ is selected from the group of CONH2, H2NCONH, dibenzylamino, phthalimido, amino, alkylamino, dialkylamino, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl where azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and is substituted with 0-3 alkyl or alkoxycarbonyl substituents;

R²⁰ is hydrogen, halo, alkyl, or alkoxy;

R²¹ is hydrogen, halo, alkyl, or alkoxy;

Ar¹ is selected from the group of phenyl, naphthalenyl, quinolinyl, isoquinolinyl, quinoxalinyl, quinazolinyl, pyridinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, oxadiathiazolyl, triazolyl, tetrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazolyl, indolyl, azaindolyl, indazolyl, azaindazolyl, benzoxazolyl, benzoisoaxazolyl, benzoisorhiazolyl, benzimidazolyl, and benzothiazolyl, and is substituted with 0-2 substituents selected from the group of halo, alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, aminocarbonyl, pyridinyl, phenyl, halophenyl, alkylphenyl, and alkoxyphenyl;

Ar² is selected from the group of phenyl, naphthalenyl, quinolinyl, isoquinolinyl, quinoxalinyl, quinazolinyl, pyridinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, oxadiathiazolyl, triazolyl, tetrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazolyl, indolyl, azaindolyl, indazolyl, azaindazolyl, benzoxazolyl, benzoisoaxazolyl, benzoisorhiazolyl, benzimidazolyl, and benzothiazolyl, and is substituted with 0-2 substituents selected from the group of halo, alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, aminocarbonyl, pyridinyl, phenyl, halophenyl, alkylphenyl, and alkoxyphenyl, and Ar⁶;

Ar³ is selected from the group of phenyl, naphthalenyl, quinolinyl, isoquinolinyl, quinoxalinyl, quinazolinyl, pyridinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, oxadiathiazolyl, triazolyl, tetrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazolyl, indolyl, azaindolyl, indazolyl, azaindazolyl, benzoxazolyl, benzoisoaxazolyl, benzoisorhiazolyl, benzimidazolyl, and benzothiazolyl, and is substituted with 0-2 substituents selected from the group of halo, alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, aminocarbonyl, pyridinyl, phenyl, halophenyl, alkylphenyl, and alkoxyphenyl; and

Ar⁴ is selected from the group of furanyl, thienyl, pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, triazolyl, pyridinyl, indolyl, and phenyl, and is substituted with 0-2 substituents selected from the group of halo, alkyl, haloalkyl, hydroxyl, and alkoxy.

The invention also relates to pharmaceutical compositions comprising a compound of Formula I, including a pharmaceutically acceptable salt thereof , and a pharmaceutically acceptable carrier, excipient and/or diluent.

In addition, the invention provides one or more methods of treating hepatitis C infection comprising administering a therapeutically effective amount of a compound of Formula I to a patient.

Also provided as part of the invention are one or more methods for making the compounds of Formula I. The present invention is directed to these, as well as other important ends, hereinafter described.

DETAILED DESCRIPTION OF THE EMBODIMENTS

The singular forms "a", "an", and "the" include plural reference unless the context clearly dictates otherwise.

Unless otherwise specifically set forth elsewhere in the application, the following terms can be used herein and shall have the following meanings: "Hydrogen" or "H" refers to hydrogen, including its isotopes, such as deuterium. "Halo" means fluoro, chloro, bromo, or iodo. "Alkyl" means a straight or branched alkyl group composed of 1 to 6 carbons. "Alkenyl" means a straight or branched alkyl group composed of 2 to 6 carbons with at least one double bond. "Cycloalkyl" means a monocyclic ring system composed of 3 to 7 carbons. "Hydroxyalkyl," "alkoxy" and other terms with a substituted alkyl moiety include straight and branched isomers composed of 1 to 6 carbon atoms for the alkyl moiety. "Halo" includes all halogenated isomers from monohalo substituted to perhalo substituted in substituents defined with halo, for example, "Haloalkyl" and "haloalkoxy", "halophenyl", "halophenoxy." "Aryl" means a monocyclic or bicyclic aromatic hydrocarbon groups having 6 to 12 carbon atoms, or a bicyclic fused ring system wherein one or both of the rings is a phenyl group. Bicyclic fused ring systems consist of a phenyl group fused to a four- to six-membered aromatic or non-aromatic carbocyclic ring. Representative examples of aryl groups include, but are not limited to, indanyl, indenyl, naphthyl, phenyl, and tetrahydronaphthyl. "Heteroaryl" means a 5 to 7 membered monocyclic or 8 to 11 membered bicyclic aromatic ring system with 1 -5 heteroatoms independently selected from nitrogen, oxygen, and sulfur. Parenthetic and multiparenthetic terms are intended to clarify bonding relationships to those skilled in the art. For example, a term such as ((R)alkyl) means an alkyl substituent further substituted with the substituent R. Substituents which are illustrated by chemical drawing to bond at variable positions on a multiple ring system (for example a bicyclic ring system) are intended to bond to the ring where they are drawn to append.

The invention includes all pharmaceutically acceptable salt forms of the compounds. Pharmaceutically acceptable salts are those in which the counter ions do not contribute significantly to the physiological activity or toxicity of the compounds and as such function as pharmacological equivalents. These salts can be made according to common organic techniques employing commercially available reagents. Some anionic salt forms include acetate, acistrate, besylate, bromide, camsylate, chloride, citrate, fumarate, glucouronate, hydrobromide, hydrochloride, hydroiodide, iodide, lactate, maleate, mesylate, nitrate, pamoate, phosphate, succinate, sulfate, tartrate, tosylate, and xinofoate. Some cationic salt forms include ammonium, aluminum, benzathine, bismuth, calcium, choline, diethylamine, diethanolamine, lithium, magnesium, meglumine, 4-phenylcyclohexylamine, piperazine, potassium, sodium, tromethamine, and zinc.

Some of the compounds of the invention possess asymmetric carbon atoms. The invention includes all stereoisomeric forms, including enantiomers and diastereomers as well as mixtures of stereoisomers such as racemates. Some stereoisomers can be made using methods known in the art. Stereoisomeric mixtures of the compounds and related intermediates can be separated into individual isomers according to methods commonly known in the art. The use of wedges or hashes in the depictions of molecular structures in the following schemes and tables is intended only to indicate relative stereochemistry, and should not be interpreted as implying absolute stereochemical assignments. The invention is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include deuterium and tritium. Isotopes of carbon include ¹ C and ¹⁴C. Isotopically- labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopically-labeled reagent in place of the non-labeled reagent otherwise employed. Such compounds can have a variety of potential uses, for example as standards and reagents in determining biological activity. In the case of stable isotopes, such compounds can have the potential to favorably modify biological, pharmacological, or pharmacokinetic properties.

As set forth above, the invention is directed to one or more compounds of Formula I, including pharmaceutically acceptable salts thereof:

I

wherein R¹, R² are each independently selected from the group of hydrogen, halo, nitro, alkyl, cycloalkyl, haloalkyl, aminoalkyl, hydroxyalkyl, alkoxyalkyl, hydroxyl, alkoxy, OR^17, cycloalkoxy, amino, alkylamino, dialkylamino, alkylcarboxamido, alkoxycarboxamido, alkoxyalkylcarboxamido and Ar¹;

R³ is selected from the group of cyano, alkoxy carbonyl, (cycloalkyl)oxycarbonyl, (alkylsulfonyl)aminocarbonyl, CONRⁿR¹², (Rⁿ)(R¹²)NCONH, triazolyl, thiazolyl, and tetrazolyl;

R⁴ is phenyl substituted with 0-2 halo substituents;

(halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl, (cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl,

(hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and

(alkoxycycloalkyl)cycloalkyl;

(halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl,

(cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl,

(hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and

(alkoxy cycloalkyl)cycloalkyl;

R⁷R⁸N taken together is a fused bicyclic ring, spiro bicyclic ring or a bridged bi cyclic ring constructed from carbon and from 1- 4 atoms selected from nitrogen, sulfur and oxygen atoms, wherein said ring is substituted with 0-5 substituents selected from the group of alkyl, hydroxyalkyl, alkoxyalkyl, hydroxy, alkoxy, carboxy, alkoxy carbonyl, dialkylcarboxamido, alkylcarbonylamino, alkoxycarbonylamino, halo, haloalkyl, cyano, cyanoalkyl, cyanoalkenyl, cyanocycloalkyl, cyanocycloalkenyl, halocycloalkyl, haloalkenyl, halocycloalkenyl, cycloalkyl, alkenyl, alkynyl, ester, amide, acid, amine, alcohol, ether, cycloether, cyclic amine, lactame, lactone, cycloalkenyl, sulfonyl, aminosulfonyl, amidinyl, guanidinyl, carbamide, urea, and Ar²;

aminocycloalkyl;

R¹⁸ is selected from the group of CONH2, H2NCONH, dibenzylamino, phthalimido, amino, alkylamino, dialkylamino, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, mo holinyl where azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, and morpholinyl, and is substituted with 0-3 alkyl or alkoxy carbonyl substituents;

R²⁰ is hydrogen, halo, alkyl, or alkoxy;

R²¹ is hydrogen, halo, alkyl, or alkoxy;

It is preferred that R¹ and R² are each hydrogen.

It is also preferred that R³ is -CONRⁿ,R¹². R¹¹ and R¹² are each independently preferred to be hydrogen or alkyl. In addition, it is preferred that R⁴ is a phenyl group substituted with one fluoro (F) group.

It is further preferred that R⁵ is alkyl or haloalkyl, and R⁶ is alkylsulfonyl.

It is also preferred that R⁷R⁸N taken together is a fused bicyclic ring with 1-4 nitrogen atoms. In another embodiment, it is preferred that R⁷R⁸N taken together have 2- 4 nitrogen atoms.

In a further embodiment, it is preferred that R²⁰ and R²¹ are each hydrogen.

Also preferred are compounds of Formula I, including pharmaceutically acceptable salts thereof, which are selected from the group of:

Pharmaceutical Compositions and Methods of Treatment

The compounds according to the various embodiments herein set forth demonstrate activity against HCV NS5B, and can be useful in treating HCV and HCV infection. Therefore, another aspect of the invention is a composition comprising a compound of Formula I, or a pharmaceutically acceptable salt thereof, and a

pharmaceutically acceptable carrier, excipient and/or diluent.

Another aspect of the invention is a composition further comprising an additional compound having anti-HCV activity.

Another aspect of the invention is a composition where the compound having anti- HCV activity is an interferon or a ribavirin. Another aspect of the invention is wherein the interferon is selected from interferon alpha 2B, pegylated interferon alpha, consensus interferon, interferon alpha 2A, interferon lambda, and lymphoblastoid interferon tau. Another aspect of the invention is a composition where the compound having anti HCV activity is a cyclosporin. Another aspect of the invention is where the cyclosporin is cyclosporin A.

Another aspect of the invention is a composition where the compound having anti HCV activity is selected from the group consisting of interleukin 2, interleukin 6, interleukin 12, a compound that enhances the development of a type 1 helper T cell response, interfering RNA, anti-sense RNA, Imiqimod, ribavirin, an inosine 5'- monophospate dehydrogenase inhibitor, amantadine, and rimantadine.

Another aspect of the invention is a composition where the compound having anti HCV activity is effective to inhibit the function of a target selected from HCV metalloprotease, HCV serine protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, IMPDH, and a nucleoside analog for the treatment of an HCV infection.

Another aspect of the invention is a composition comprising a compound of Formula I, or a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable carrier, excipient and/or diluent, an interferon and ribavirin.

Another aspect of the invention is a method of inhibiting the function of the HCV replicon comprising contacting the HCV replicon with a compound of Formula I or a pharmaceutically acceptable salt thereof.

Another aspect of the invention is a method of inhibiting the function of the HCV NS5B protein comprising contacting the HCV NS5B protein with a compound of Formula I or a pharmaceutically acceptable salt thereof.

Another aspect of the invention is a method of treating an HCV infection in a patient comprising administering to the patient a therapeutically effective amount of a compound of Formula I or a pharmaceutically acceptable salt thereof. In another embodiment the compound is effective to inhibit the function of the HCV replicon. In another embodiment the compound is effective to inhibit the function of the HCV NS5B protein.

Another aspect of the invention is a method of treating an HCV infection in a patient comprising administering to the patient a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof, in conjunction with (prior to, after, or concurrently) another compound having anti-HCV activity.

Another aspect of the invention is the method wherein the other compound having anti-HCV activity is an interferon or a ribavirin.

Another aspect of the invention is the method where the interferon is selected from interferon alpha 2B, pegylated interferon alpha, consensus interferon, interferon alpha 2A, interferon lambda, and lymphoblastoid interferon tau.

Another aspect of the invention is the method where the other compound having anti-HCV activity is a cyclosporin.

Another aspect of the invention is the method where the cyclosporin is cyclosporin A.

Another aspect of the invention is the method where the other compound having anti-HCV activity is selected from interleukin 2, interleukin 6, interleukin 12, a compound that enhances the development of a type 1 helper T cell response, interfering RNA, anti-sense RNA, Imiqimod, ribavirin, an inosine 5'-monophospate dehydrogenase inhibitor, amantadine, and rimantadine.

Another aspect of the invention is the method wherein the other compound having anti-HCV activity is effective to inhibit the function of a target selected from the group consisting of HCV metalloprotease, HCV serine protease, HCV polymerase, HCV helicase, HCV NS4B protein, HCV entry, HCV assembly, HCV egress, HCV NS5A protein, IMPDH, and a nucleoside analog for the treatment of an HCV infection. Another aspect of the invention is the method wherein the other compound having anti-HCV activity is effective to inhibit the function of target in the HCV life cycle other than the HCV NS5B protein. "Therapeutically effective" means the amount of agent required to provide a meaningful patient benefit as understood by practitioners in the field of hepatitis and HCV infection.

"Patient" means a person infected with the HCV virus and suitable for therapy as understood by practitioners in the field of hepatitis and HCV infection.

"Treatment," "therapy," "regimen," "HCV infection," and related terms are used as understood by practitioners in the field of hepatitis and HCV infection. The compounds of this invention are generally given as pharmaceutical compositions comprised of a therapeutically effective amount of a compound or its pharmaceutically acceptable salt and a pharmaceutically acceptable carrier, excipient and/or diluent and can contain conventional excipients. Pharmaceutically acceptable carriers are those conventionally known carriers having acceptable safety profiles.

Compositions encompass all common solid and liquid forms including for example capsules, tablets, lozenges, and powders as well as liquid suspensions, syrups, elixers, and solutions. Compositions are made using common formulation techniques, and conventional excipients (such as binding and wetting agents) and vehicles (such as water and alcohols) are generally used for compositions. See, for example, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, PA, 17th edition, 1985.

Solid compositions are normally formulated in dosage units and compositions providing from about 1 to 1000 mg of the active ingredient per dose are preferred. Some examples of dosages are 1 mg, 10 mg, 100 mg, 250 mg, 500 mg, and 1000 mg.

Generally, other agents will be present in a unit range similar to agents of that class used clinically. Typically, this is 0.25-1000 mg/unit. Liquid compositions are usually in dosage unit ranges. Generally, the liquid composition will be in a unit dosage range of 1-100 mg/mL. Some examples of dosages are 1 mg/mL, 10 mg/mL, 25 mg/mL, 50 mg/mL, and 100 mg/mL. Generally, other agents will be present in a unit range similar to agents of that class used clinically.

Typically, this is 1-100 mg/mL.

The invention encompasses all conventional modes of administration; oral and parenteral methods are preferred. Generally, the dosing regimen will be similar to other agents used clinically. Typically, the daily dose will be 1-100 mg/kg body weight daily. Generally, more compound is required orally and less parenterally. The specific dosing regimen, however, will be determined by a physician using sound medical judgment.

The invention also encompasses methods where the compound is given in combination therapy. That is, the compound can be used in conjunction with, but separately from, other agents useful in treating hepatitis and HCV infection. In these combination methods, the compound will generally be given in a daily dose of 1-100 mg/kg body weight daily in conjunction with other agents. The other agents generally will be given in the amounts used therapeutically. The specific dosing regimen, however, will be determined by a physician using sound medical judgment. Some examples of compounds suitable for compositions and methods are listed in

Table 1.

Table 1

Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

ISIS

Pharmaceuticals Inc,

ISIS 14803 Antiviral antisense Carlsbad, CA/Elan

Pharmaceuticals Inc., New York, NY

Endo

Pharmaceuticals

Summetrel Antiviral antiviral

Holdings Inc., Chadds Ford, PA

GS-9132 (ACH-

Antiviral HCV Inhibitor Achillion / Gilead 806)

Pyrazolopyrimidine

compounds and

salts Arrow Therapeutics

Antiviral HCV Inhibitors

From WO- Ltd.

2005047288

26 May 2005

Ribapharm Inc.,

Levovirin Antiviral IMPDH inhibitor

Costa Mesa, CA

Vertex

Merimepodib Pharmaceuticals

Antiviral IMPDH inhibitor

(VX-497) Inc., Cambridge,

MA

XTL

XTL-6865 (XTL-

Antiviral monoclonal antibody Biopharmaceuticals 002)

Ltd., Rehovot, Israel

Vertex

Pharmaceuticals

Telaprevir

NS3 serine protease Inc., Cambridge, (VX-950, LY- Antiviral

inhibitor MA/ Eli Lilly and 570310)

Co. Inc.,

Indianapolis, IN Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

NS5B Replicase

HCV-796 Antiviral Wyeth / Viropharma

Inhibitor

NS5B Replicase

NM-283 Antiviral Idenix / Novartis

Inhibitor

NS5B Replicase Gene Labs /

GL-59728 Antiviral

Inhibitor Novartis

NS5B Replicase Gene Labs /

GL-60667 Antiviral

Inhibitor Novartis

NS5B Replicase

2'C MeA Antiviral Gilead

Inhibitor

NS5B Replicase

PSI 6130 Antiviral Roche

Inhibitor

NS5B Replicase

R1626 Antiviral Roche

Inhibitor

2'C Methyl NS5B Replicase

Antiviral Merck

adenosine Inhibitor

Japan Tobacco Inc.,

JTK-003 Antiviral RdRp inhibitor

Tokyo, Japan

ICN

Levovirin Antiviral ribavirin Pharmaceuticals,

Costa Mesa, CA

Schering-Plough

Ribavirin Antiviral ribavirin Corporation,

Kenilworth, NJ

Ribapharm Inc.,

Viramidine Antiviral Ribavirin Prodrug

Costa Mesa, CA

Ribozyme

Heptazyme Antiviral ribozyme Pharmaceuticals

Inc., Boulder, CO

Boehringer serine protease Ingelheim Pharma

BILN-2061 Antiviral

inhibitor KG, Ingelheim,

Germany Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

serine protease

SCH 503034 Antiviral Schering Plough inhibitor

SciClone

Zadazim Immune modulator Immune modulator Pharmaceuticals

Inc., San Mateo, CA

Maxim

Ceplene Immunomodulator immune modulator Pharmaceuticals

Inc., San Diego, CA

F. Hoffmann-La

HCV IgG immuno¬

CellCept Immunosuppressant Roche LTD, Basel, suppressant

Switzerland

Nabi

HCV IgG immuno¬

Civacir Immunosuppressant Biopharmaceuticals suppressant

Inc., Boca Raton, FL

Human Genome

Albuferon - a Interferon albumin IFN-a2b Sciences Inc.,

Rockville, MD

InterMune

IFN

Infergen A Interferon Pharmaceuticals alfacon-1

Inc., Brisbane, CA

Omega IFN Interferon IFN-co Intarcia Therapeutics

Transition

IFN-P and EMZ701 Interferon IFN- and EMZ701 Therapeutics Inc.,

Ontario, Canada

Serono, Geneva,

Rebif Interferon IFN- la

Switzerland

F. Hoffmann-La

Roferon A Interferon IFN-a2a Roche LTD, Basel,

Switzerland

Schering-Plough

Intron A Interferon IFN-a2b Corporation,

Kenilworth, NJ Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

RegeneRx

Biopharma. Inc.,

Intron A and Bethesda, MD/

Interferon IFN-a2b/a 1 -thymosin Zadaxin SciClone

Pharmaceuticals Inc, San Mateo, CA

Schering-Plough

Rebetron Interferon IFN-a2b/ribavirin Corporation,

Kenilworth, NJ

InterMune Inc.,

Actimmune Interferon INF-γ

Brisbane, CA

Interferon- β Interferon Interferon- -la Serono

Viragen/

Multiferon Interferon Long lasting IFN

Valentis

Lympho-blastoid IFN- GlaxoSmithKline

Wellferon Interferon

anl pic, Uxbridge, UK

Viragen Inc.,

Omniferon Interferon natural IFN-a

Plantation, FL

F. Hoffmann-La

Pegasys Interferon PEGylated IFN-a2a Roche LTD, Basel,

Switzerland

Maxim

Pegasys and PEGylated IFN-a2a/

Interferon Pharmaceuticals Ceplene immune modulator

Inc., San Diego, CA

F. Hoffmann-La

Pegasys and PEGylated IFN-

Interferon Roche LTD, Basel, Ribavirin a2a/ribavirin

Switzerland

Schering-Plough

PEG-Intron Interferon PEGylated IFN-a2b Corporation,

Kenilworth, NJ

Schering-Plough

PEG-Intron / PEGylated IFN-

Interferon Corporation, Ribavirin a2b/ribavirin

Kenilworth, NJ Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

Indevus

IP-501 Liver protection antifibrotic Pharmaceuticals

Inc., Lexington, MA

Idun

IDN-6556 Liver protection caspase inhibitor Pharmaceuticals

Inc., San Diego, CA

InterMune serine protease

ITMN-191 (R-7227) Antiviral Pharmaceuticals inhibitor

Inc., Brisbane, CA

NS5B Replicase

GL-59728 Antiviral Genelabs

Inhibitor

ANA-971 Antiviral TLR-7 agonist Anadys

serine protease

Boceprevir Antiviral Schering Plough inhibitor

serine protease Tibotec BVBA,

TMS-435 Antiviral

inhibitor Mechelen, Belgium

Boehringer serine protease Ingelheim Pharma

BI-201335 Antiviral

inhibitor KG, Ingelheim,

Germany serine protease

MK-7009 Antiviral Merck

inhibitor

PF-00868554 Antiviral replicase inhibitor Pfizer

Anadys

Non-Nucleoside

Pharmaceuticals,

ANA598 Antiviral NS5B Polymerase

Inc., San Diego, CA, Inhibitor

USA

Idenix

Non-Nucleoside Pharmaceuticals,

IDX375 Antiviral

Replicase Inhibitor Cambridge, MA,

USA Type of Inhibitor or

Brand Name Physiological Class Source Company

Target

Boehringer

NS5B Polymerase Ingelheim Canada

BILB 1941 Antiviral

Inhibitor Ltd R&D, Laval,

QC, Canada

Nucleoside Pharmasset,

PSI-7851 Antiviral

Polymerase Inhibitor Princeton, NJ, USA

Nucleotide NS5B Pharmasset,

PSI-7977 Antiviral

Polymerase Inhibitor Princeton, NJ, USA

Antiviral NS5B Polymerase

VCH-759 ViroChem Pharma

Inhibitor

Antiviral NS5B Polymerase

VCH-916 ViroChem Pharma

Inhibitor

Antiviral NS5B Polymerase

GS-9190 Gilead

Inhibitor

Peg-interferon Antiviral ZymoGenetics/Brist

Interferon

lambda ol-Myers Squibb

Synthesis Methods The compounds can be made by methods known in the art, including those described below. Some reagents and intermediates are known in the art. Other reagents and intermediates can be made by methods known in the art using commercially available materials. The variables (e.g. numbered "R" substituents) used to describe the synthesis of the compounds are intended only to illustrate how to make and are not to be confused with variables used in the claims or in other sections of the specification. Abbreviations used within the schemes generally follow conventions used in the art.

Abbreviations used in the schemes generally follow conventions used in the art. Chemical abbreviations used in the specification and examples are defined as follows: "NaHMDS" for sodium bis(trimethylsilyl)amide; "DMF" for N,N-dimethylformamide; "MeOH" for methanol; "NBS" for N-bromosuccinimide; "Ar" for aryl; "TFA" for trifluoroacetic acid; "LAH" for lithium aluminum hydride; "DMSO" for

dimethylsulfoxide; "h" for hours; "rt" for room temperature or retention time (context will dictate); "min" for minutes; "EtOAc" for ethyl acetate; "THF" for tetrahydrofuran; "EDTA" for ethylenediaminetetraacetic acid; "Et20" for diethyl ether; "DMAP" for 4- dimethylaminopyridine; "DCE" for 1,2-dichloroethane; "ACN" for acetonitrile; "DME" for 1 ,2-dimethoxy ethane; "HOBt" for 1-hydroxybenzotriazole hydrate; "DIEA" for diisopropylethylamine.

For the section of compounds in the 0000 series all Liquid Chromatography (LC) data were recorded on a Shimadzu LC-10AS or LC-20AS liquid chromotograph using a SPD-10AV or SPD-20A UV-Vis detector and Mass Spectrometry (MS) data were determined with a Micromass Platform for LC in electrospray mode.

HPLC Method (i.e., compound isolation). Compounds purified by preparative HPLC were diluted in methanol (1.2 mL) and purified using a Shimadzu LC-8A or LC- 10A or Dionex APS-3000 or Waters Acquity™ automated preparative HPLC system.

Examples:

Preparation of intermediate 3:

Step 1 : 2- Iodoethane (0.236 g) and CS2CO3 (0.492 g) were added into a solution of methyl 3-(2-(4-fluorophenyl)-3-(methylcarbamoyl)-6-(methylsulfonamido)benzofuran-5- yl)benzoate (0.25 g) in DMF (5 mL). The reaction was stirred at room temperature for 16 hours. After removal of solvents under vacuum, the residue was purified by preparative HPLC system. Compound 2

MS (M+H)⁺ Calcd. 525.1

MS (M+H)⁺ Observ. 525.0

Retention Time 2.04 min

LC Condition

Solvent A 90% Water -10% Methanol-0.1% TFA

Solvent B 10% Water -90% Methanol-0.1% TFA

Start % B 0

Final % B 100

Gradient Time 2 min

Flow Rate 1 mL/min

Wavelength 220

Solvent Pair Water - Methanol- TFA

Column PHENOMENEX-LU A 2.0 x 30mm 3um

Step 2: K2CO3 (0.020 g) was added into a solution of Compound 2 (0.025 g) in methanol (3 mL) and water (1 mL). The reaction was heated at 70°C for 1 hour. After removal of solvents under vacuum, the residue was purified by preparative HPLC system to give Compound 3.

Solvent Pair Water - Methanol- TFA

Column PHENOMENEX-LU A 2.0 x 30mm 3um

Preparation of Intermediate 5:

Step 1 : Compound 4 was prepared via the same process of synthesizing Compound 2, using l-fluoro-2-iodoethane as starting material.

Step 2: Compound 5 was prepared via the same process of synthesizing Compound 3, using Compound 4 as starting material.

MS (M+H)⁺ Observ. 529.1

Retention Time 3.38 min

LC Condition

Solvent A 90% Water -10% Methanol-0.1% TFA

Solvent B 10% Water -90% Methanol-0.1% TFA

Start % B 0

Final % B 100

Gradient Time 4 min

Flow Rate 0.8 mL/min

Wavelength 220

Solvent Pair Water - Methanol- TFA

Column PHENOMENEX-LU A 2.0 X 50mm 3um

General procedure of amide formation, preparation of 1001-1010: iPr₂NEt or Et₃N (2 eq.) and HATU or HCTU or DEBPT (1.3 eq.) were added into a solution of Compound 5 (1 eq.) and amine (1.3 eq.) in DMF or THF. The reaction was stirred at room temperature or 85°C for 30 minutes to 72 hours. The desired product was isolated by preparative HPLC system.

LC Condition B

Solvent A 5 % ACN: 95% Water : lOmM Ammonium Acetate

Solvent B 95 % ACN: 5% Water : lOmM Ammonium Acetate

Start % B 0

Final % B 100

Gradient Time 2 min

Flow Rate 1 mL/min

Wavelength 220

Solvent Pair ACN: Water: Ammonium Acetate

Column Phenomenex LUNA CI 8, 30x2, 3u

MS MS Retention

Cmpd LC

Structure (M+H)⁺ (M+H)⁺ Time

# Method

Calcd. Observ. (min)

1001 A 702.2 702.3 2.05

-29-

Biological Methods

The compound demonstrated activity against HCV NS5B as determined in the following HCV RdRp assays.

HCVNS5B RdRp cloning, expression, and purification. The cDNA encoding NS5B proteins of HCV genotype lb (Conl), a genotype lb variant with amino acid 316 mutated from cysteine to asparagine, and genotype 2a (JFH-1), were cloned into the pET2 la expression vector. Each untagged protein was expressed with an 18 amino acid C-terminal truncation to enhance the solubility. The E. coli competent cell line

BL21(DE3) was used for expression of the protein. Cultures were grown at 37 °C for ~ 4 hours until the cultures reached an optical density of 2.0 at 600 nm. The cultures were cooled to 20 °C and induced with 1 mM IPTG. Fresh ampicillin was added to a final concentration of 50 μg/mL and the cells were grown overnight at 20 °C.

Cell pellets (3L) were lysed for purification to yield 15-24 mgs of purified NS5B. The lysis buffer consisted of 20 mM Tris-HCl, pH 7.4, 500 mM NaCl, 0.5% triton X-100, 1 mM DTT, ImM EDTA, 20% glycerol, 0.5 mg/mL lysozyme, 10 mM MgCb, 15 ug/mL deoxyribonuclease I, and Complete TM protease inhibitor tablets (Roche). After addition of the lysis buffer, frozen cell pellets were resuspended using a tissue homogenizer. To reduce the viscosity of the sample, aliquots of the lysate were sonicated on ice using a microtip attached to a Branson sonicator. The sonicated lysate was centrifuged at 100,000 x g for 30 minutes at 4 °C and filtered through a 0.2 μπι filter unit (Coming). The protein was purified using two sequential chromatography steps: Heparin sepharose CL-6B and polyU sepharose 4B. The chromatography buffers were identical to the lysis buffer but contained no lysozyme, deoxyribonuclease I, MgCb or protease inhibitor and the NaCl concentration of the buffer was adjusted according to the requirements for charging the protein onto the column. Each column was eluted with a NaCl gradient which varied in length from 5-50 column volumes depending on the column type. After the final chromatography step, the resulting purity of the enzyme is >90% based on SDS-PAGE analysis. The enzyme was aliquoted and stored at -80 °C. HCVNS5B RdRp enzyme assay. An on-bead solid phase homogeneous assay was used in a 384-well format to assess NS5B inhibitors (WangY-K, Rigat K, Roberts S, and Gao M (2006) Anal Biochem, 359: 106-111). The biotinylated oligo dTi2 primer was captured on streptavidin-coupled imaging beads (GE, RPNQ0261) by mixing primer and beads in IX buffer and incubating at room temperature for three hours. Unbound primer was removed after centrifugation. The primer-bound beads were resuspended in 3X reaction mix (20 mM Hepes buffer, pH 7.5, dT primer coupled beads, poly A template, H-UTP, and RNAse inhibitor (Promega N2515)). Compounds were serially diluted 1 :3 in DMSO and aliquoted into assay plates. Equal volumes (5 of water, 3X reaction mix, and enzyme in 3X assay buffer (60 mM Hepes buffer, pH 7.5, 7.5 mM MgCh, 7.5 mM KC1, 3 mM DTT, 0.03 mg/mL BSA, 6 % glycerol) were added to the diluted compound on the assay plate. Final concentration of components in 384-well assay: 0.36 nM template, 15 nM primer, 0.29 μΜ ¾-UTP (0.3 μ(¾, 1.6 υ/μί RNAse inhibitor, 7 nM NS5B enzyme, 0.01 mg/mL BSA, 1 mM DTT, and 0.33 μg/μL beads, 20 mM Hepes buffer, pH 7.5, 2.5 mM MgCh, 2.5 mM KC1, and 0.1 % DMSO.

Reactions were allowed to proceed for 24 hours at 30° C and terminated by the addition of 50 mM EDTA (5 After incubating for at least 15 minutes, plates were read on an Amersham LEADseeker multimodality imaging system.

IC50 values for compounds were determined using ten different [I]. IC50 values were calculated from the inhibition using the four-parameter logistic formula y = A + ((B- A)/(l+((C/x)^AD))), where A and B denote minimal and maximal % inhibition, respectively, C is the IC50, D is hill slope and x represents compound concentration.

Cell lines. The cell lines used to evaluate compounds consist of a human hepatocyte derived cell line (Huh-7) that constitutively expresses a genotype lb (Con-1) HCV replicon or a genotype lb (Con-1) HCV replicon with an asparagine replacing the cysteine at amino acid 316, or a genotype 2a (JFH-1) replicon, containing a Renilla luciferase reporter gene. These cells were maintained in Dulbecco's modified Eagle medium (DMEM) containing 10% FBS, 100 U/mL penicillin/streptomycin and 1.0 mg/mL G418.

HCV replicon luciferase assay. To evaluate compound efficacy, titrated compounds were transferred to sterile 384-well tissue culture treated plates, and the plates were seeded with HCV replicon cells (50 at a density of 2.4 x 10³ cells/well) in DMEM containing 4 % FBS (final DMSO concentration at 0.5 %). After 3 days incubation at 37°C, cells were analyzed for Renilla Luciferase activity using the EnduRen substrate (Promega cat #E6485) according to the manufacturer's directions. Briefly, the EnduRen substrate was diluted in DMEM and then added to the plates to a final concentration of 7.5 μΜ. The plates were incubated for at least 1 h at 37°C then read on a Viewlux Imager (PerkinElmer) using a luminescence program. The 50% effective concentration (EC50) was calculated using the four-parameter logistic formula noted above.

To assess cytotoxicity of compounds, Cell Titer-Blue (Promega) was added to the EnduRen-containing plates and incubated for at least 4 hrs at 37°C. The fluorescence signal from each well was read using a Viewlux Imager. All CC50 values were calculated using the four-parameter logistic formula. Compound EC50 data is expressed as A: < 100 nM; B = 100-1000 nM; C > 1000 nM). Representative data for compounds are reported in Table 2.

Table 2

It will be evident to one skilled in the art that the present disclosure is not limited to the foregoing illustrative examples, and that it can be embodied in other specific forms without departing from the essential attributes thereof. It is therefore desired that the examples be considered in all respects as illustrative and not restrictive, reference being made to the appended claims, rather than to the foregoing examples, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

CLAIMS We claim:

1. A compound of Formula I, including pharmaceutically acceptable salts thereof:

I

wherein R¹, R² are each independently selected from the group of hydrogen, halo, nitro, alkyl, cycloalkyl, haloalkyl, aminoalkyl, hydroxyalkyl, alkoxyalkyl, hydroxyl, alkoxy, OR^17, cycloalkoxy, amino, alkylamino, dialkylamino,

alkylcarboxamido, alkoxy carboxamido, alkoxyalkylcarboxamido and Ar¹;

R³ is selected from the group of cyano, alkoxy carbonyl,

(cycloalkyl)oxycarbonyl, (alkylsulfonyl)aminocarbonyl, CONRⁿR¹²,

(Rⁿ)(R¹²)NCONH, triazolyl, thiazolyl, and tetrazolyl;

R⁴ is phenyl substituted with 0-2 halo substituents;

(halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl,

(cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl,

(hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and (alkoxy cycloalkyl)cycloalkyl;

R⁶ is selected from the group of hydrogen, alkyl, hydroxyalkyl, alkoxyalkyl, alkylsulfonyl, alkenyl, alkynyl, haloalkyl, haloalkenyl, haloalkynyl, cyanoalkyl, cyanoalkenyl, cyanoalkynyl, cycloalkyl, halocycloalkyl, (haloalkyl)cycloalkyl, (halocycloalkyl)cycloalkyl, cyanocycloalkyl, (cyanoalkyl)cycloalkyl,

(cyanocycloalkyl)cycloalkyl, hydroxy cycloalkyl, (hydroxyalkyl)cycloalkyl, (hydroxy cycloalkyl)cycloalkyl alkoxy cycloalkyl, (alkoxyalkyl)cycloalkyl, and (alkoxycycloalkyl)cycloalkyl;

R⁷R⁸N taken together is a fused bicyclic ring, spiro bicyclic ring or a bridged bi cyclic ring constructed from carbon and from 1- 4 atoms selected from nitrogen, sulfur and oxygen atoms, wherein said ring is substituted with 0-5 substituents selected from the group of alkyl, hydroxyalkyl, alkoxyalkyl, hydroxy, alkoxy, carboxy, alkoxycarbonyl, dialkylcarboxamido, alkylcarbonylamino,

alkoxycarbonylamino, halo, haloalkyl, cyano, cyanoalkyl, cyanoalkenyl,

cyanocycloalkyl, cyanocycloalkenyl, halocycloalkyl, haloalkenyl, halocycloalkenyl, cycloalkyl, alkenyl, alkynyl, ester, amide, acid, amine, alcohol, ether, cycloether, cyclic amine, lactame, lactone, cycloalkenyl, sulfonyl, aminosulfonyl, amidinyl, guanidinyl, carbamide, urea, and Ar²;

R¹⁷ is selected from the group of haloalkyl, cyanoalkyl, (cycloalkyl)alkyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, (R¹⁸)alkyl, (Ar⁴)alkyl, alkynyl, and aminocycloalkyl;

R²⁰ is hydrogen, halo, alkyl, or alkoxy;

R²¹ is hydrogen, halo, alkyl, or alkoxy;

Ar is selected from the group of phenyl, naphthalenyl, quinolinyl, isoquinolinyl, quinoxalinyl, quinazolinyl, pyridinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, isoxazolyl, isothiazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, oxadiathiazolyl, triazolyl, tetrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazolyl, indolyl, azaindolyl, indazolyl, azaindazolyl, benzoxazolyl, benzoisoaxazolyl, benzoisorhiazolyl, benzimidazolyl, and benzothiazolyl, and is substituted with 0-2 substituents selected from the group of halo, alkyl, cycloalkyl, haloalkyl, alkoxyalkyl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, aminocarbonyl, pyridinyl, phenyl, halophenyl, alkylphenyl, and alkoxyphenyl; and

2. A compound of claim 1 wherein R¹ and R² are each hydrogen.

3. A compound of claim 2 wherein R⁴ is phenyl substituted with one fluoro group.

4. A compound of claim 3 wherein R³ is -CONRⁿR¹².

5. A compound of claim 4 wherein R¹¹ and R¹² are each independently hydrogen or alkyl.

6. A compound of claim 2 wherein R⁵ is alkyl or haloalkyl.

7. A compound of claim 6 wherein R⁶ is alkylsulfonyl.

8. A compound of claim 2 wherein R⁷R⁸N is a fused bicyclic ring nitrogen atoms.

9. A compound of claim 8 wherein R⁷R⁸N has 2-4 nitrogen atoms.

10. A compound of claim 2 wherein R²⁰ is hydrogen.

11. A compound of claim 10 wherein R²¹ is hydrogen.

12. A compound, including pharmaceutically acceptable salts thereof, which is selected from the group of:

13. A composition comprising a compound of claim 1 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier, excipient and/or diluent.

14. A composition comprising a compound of claim 12 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier, excipient and/or diluent.

15. A method of treating hepatitis C infection comprising administering a therapeutically effective amount of a compound of claim 1 to a patient.