WO2016106387A2 - Nucleic acid aptamers to treat histone-induced disease states - Google Patents

Nucleic acid aptamers to treat histone-induced disease states Download PDF

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WO2016106387A2
WO2016106387A2 PCT/US2015/067516 US2015067516W WO2016106387A2 WO 2016106387 A2 WO2016106387 A2 WO 2016106387A2 US 2015067516 W US2015067516 W US 2015067516W WO 2016106387 A2 WO2016106387 A2 WO 2016106387A2
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nucleic acid
aptamer
acid molecule
rna
aptamers
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WO2016106387A3 (en
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Paloma H. Giangrande
Francis Miller
Kevin URAK
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University of Iowa Research Foundation UIRF
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/007—Pulmonary tract; Aromatherapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A61P29/02—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] without antiinflammatory effect
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

Definitions

  • a challenging medical problem often observed in critically ill patients is that in response to severe injury or illness, even those organs not. directly affected by the original problem become dysfunctional.
  • patients with severe infection will often develop respiratory failure requiring mechanical ventilation, renal failure requiring dialysis, hepatic dysfunction, coagulation abnormalities, and hypotension requiring vasopressors.
  • This condition known as the multiple organ dysfunction syndrome (MODS), or by some of its more prominent mani festations such as acute respiratory distress syndrome (ARDS), can be reversible, but the only treatment is supportive care as there is no therapy to directly prevent or reverse MODS.
  • MODS multiple organ dysfunction syndrome
  • ARDS acute respiratory distress syndrome
  • the incidence of MODS in intensive care unit (ICU) patients is 10-40% and the incidence of death in MODS is 40-50%. Even with survival, organ recovery can take months with significant associated morbidity and cost.
  • AO acute lung injury
  • ARDS acute respiratory distress syndrome
  • the present invention is transformative because it develops an innovative treatment with preventing the development of MODS/ARBS in high risk patients.
  • Extracellular histones are targeted with RNA aptamers in order to terminate the self-propagating cycle of tissue injury responsible for MO S/ARDS.
  • RNA aptamers By interrupting the cycle of histone-mediated cell injury, these histone-specific aptamers save lives and reduce the morbidity and the financial cost of multiple severe illnesses in thousands of patients.
  • nucleic acid molecule not more than 100 nucleotides in length (e.g., 50, 51 , 52, 53, 54, 55, 56, 57, 58, 59, 60. 61 , 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91 , 92, 93, 94, 95.96, 97, 98, 99, or 100 nt) comprising an aptamer, wherein the aptamer specifically targets histones.
  • the aptamer specifically targets both H3 and/or H4.
  • the aptamer specifically targets both human H3 and 114.
  • the nucleotides are RNA.
  • the nucleic acid of the present invention is DNA.
  • the RNA inciudes a modified nucleotide.
  • the nucleic acid molecule comprises a chemically modified RNA comprising a 2' substituted sugar such as 2 ' -0-methyl-; 2'-0-(2-methoxyeth l); 2-0- alkyl; 2-O-allyl; 2'-S-alkyi; 2'-S-alIyl; 2 -fluoro-; 2'-amtno; 2'-halo or 2-azido-ribose, a carbocyclic sugar analogue, an a-anomeric sugar; an epimeric sugar such as arabtnose, xylose or lyxose, a pyranose sugar, a furanose sugar, sedoheptulose 2'-£luoro ⁇ p ⁇ D-arabinonueleotide (FANA) modification: a Locked nucleic acid (LNA).
  • a 2' substituted sugar such as 2 ' -0-methyl-; 2'-0-(2-methoxyeth l); 2-0
  • the 2'OMe modified bases are alternated with un-modified RNA bases.
  • the chemically modified RNA comprises a 2'-fluoro
  • the nucleic acid molecule comprises an 2'OMe purine and a 2'-fluoro pyrimidine.
  • the nucleic acid comprises bridging phosphorothioate, and end cap that reverses the polarity of the chain and/or a linker.
  • Certain embodiments of the present invention provide a conjugate comprising the nucleic acid molecule described above linked to a therapeutic or diagnostic molecule.
  • Certain embodiments of the present invention provide a pharmaceutical composition comprising a molecule or conjugate as described above and a pharmaceutically acceptable carrier.
  • the administration is by intravenous injection or by inhalation.
  • the composition is administered within 0-24 hours after an injury.
  • the composition is administered within 0-12 hours after an injury, in certain embodiments, the injury is trauma, burn, sepsis, transfusion -related acute lung injury, organ ischemia or diagnosis of illness, or inhalation lung injury, in certain embodiments, the composition is administered in response to a prolonged severe illness.
  • the composition is administered multiple times, such as two, three, four, five or six times.
  • Certain embodiments of the present invention provide a use of a molecule or conjugate as described above for treating ODS/ARDS.
  • Certain embodiments of the present invention provide a molecule or conjugate as described above for use in therapy.
  • the disease is autoimmune disease (such as systemic lupus erythematosus), arthritis, rheumatoid arthritis, juvenile arthritis; edema; sepsis; septic shock; inflammation; non-septic hyper inflammatory disorder; infectious disease; thrombosis;
  • autoimmune disease such as systemic lupus erythematosus
  • arthritis such as systemic lupus erythematosus
  • rheumatoid arthritis juvenile arthritis
  • edema sepsis
  • septic shock inflammation
  • non-septic hyper inflammatory disorder such as infectious disease
  • infectious disease such as thrombosis
  • the method further comprising administering at least one other therapeutic agent to the subject, before, concurrently with or after the composition.
  • the present inventio further provides a nucleic acid coding molecule encoding a nucleic acid aptamer molecule as described above.
  • the therapeutic agent is selected from the group consisting of an antibody, an antibody fragment, an immune conjugate, a radionuclide, an
  • an anti-angiogenic agent a pro-apoptotic agent
  • a cytokine a chemokine
  • a drug a toxin, a hormone, an siRNA, a cytokine, a chemokine, a coagulation inhibitor, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony sttmuiating factor, an interferon, erythropoietin, thrombopoietm, an enzyme, recombinant human thrombomodulin and activated human protein C.
  • ''therapeutic effect ' ' refers to a change in the associated abnormalities of the disease state, including pathological and behavioral deficits; a change in the time to progression of the disease state; a reduction, lessening, or alteration of a symptom of the disease; or an improvement in the quality of life of the person afflicted with the disease.
  • Therapeutic effect can be measured quantitativel by a physician or qualitatively by a patient afflicted with the disease state targeted by the aptamer.
  • the term therapeutic effect defines a condition in which silencing of the wild type allele's expression does not have a deleterious or harmful effect on nonnal functions such that the patient would not have a therapeutic effect.
  • the RNA aptamers are constructed using known techniques to at least provide as operatively linked components in the direction, of transcription, control elements including a transcriptional initiation region, the DNA of interest and a
  • control elements are selected to be functional in a mammalian cell.
  • the resulting construct which contains the operatively linked components is flanked (5' and 3') with functional sequences, such as sequences encoding an aptamer.
  • compositions will comprise sufficient genetic material to produce a therapeutically effective amount of the aptamer of interest, i.e., an amount sufficient to reduce or ameliorate symptoms of the disease state in question or an amount sufficient to confer the desired benefit.
  • the pharmaceutical compositions will also contain a pharmaceutically acceptable excipient.
  • excipients include any pharmaceutical agent that does not itself induce the production of antibodies harmful to the indi vidual receiving the composition, and which may be administered without undue toxicity.
  • Pharmaceutically acceptable excipients include, but are not limited to, sorbitol, TweenSO, and liquids such as water, saline, glycerol and ethanol.
  • Pharmaceutically acceptable salts can be included therein, for example, mineral acid salts such as hydrochlorides, hydro bromides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, malonates, benzoates, and the like. Additionally, auxiliary substances, such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles.
  • mineral acid salts such as hydrochlorides, hydro bromides, phosphates, sulfates, and the like
  • organic acids such as acetates, propionates, malonates, benzoates, and the like
  • auxiliary substances such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles.
  • Figure 1 Pathways of critical illness.
  • Figures 2A-2B Extracellular histories mediate self-propagating diverse tissue injur ⁇ '.
  • Figures 3 ⁇ -3 ⁇ Binding of RNA aptamers to histone H3 and H4 proteins.
  • A Binding affinities of H3/H4 to unselected RNA aptamer library as determined by a dot blot filter binding assay. H3 j - 2.1 nM; H4 K D " 1 -5 nM.
  • FIGS 4.4 and 4B RNA aptamers inliibit hi stone-mediated platelet aggregation. Platelet aggregation was performed with washed human platelets and quantitated using an aggregometer at 2 mln intervals.
  • H3 50 mg mt -- top row
  • H4 10 mg/mL - bottom row
  • Addition of the unselected round 0 (R0) aptamer pool (10 nM) reduces platelet aggregation.
  • R0 unselected round 0
  • RNA aptamers have no effect on collagen-mediated platelet aggregation (top panels).
  • heparin HJ/mL reverses histone-mediated platelet aggregation (data shown for histone 114).
  • FIG. 1 Schematic of in vitro SELEX technology.
  • FIGS 8A-8B Sequence Enrichment data.
  • FIGS 9A-9B Bioinformatics analysis of selected rounds.
  • Figure 10 Selected aptamer sequences #1 .
  • Figure 11. Predicted secondary structure of selected aptamer sequences.
  • Figure 12. Double-filter binding assay.
  • FIGS. I3A-I3B Binding data of rounds 0 and 8 of selection.
  • Figures 14A-14B Binding data of rounds 0 and 8 of selection to calf thymus hi stones.
  • Figures 15A-15B Binding specificity of selected round 8 RNA pool.
  • FIGS 16A-16I Binding of individual RNA aptamers to hi stones H and 114.
  • Figure 7. 1 able of binding affinities.
  • FIG. J8A-18I Binding data of individual RNA aptamers to histones H I , 113 and H4.
  • Figures 20A-2 I. Binding data of individual RNA aptamers to histories I B and H4, and human serum.
  • Figure 21 Table of binding affinities.
  • Figure 23 Table of binding affinities.
  • FIGS. 24A-24C Binding data of individual RNA aptamers to histones Hi , H2A, H2B, H3 and 114 proteins.
  • Figure 25 Table of binding affinities.
  • FIG. 26 Schematic of platelet aggregometer.
  • FIGS. 27A-27D Platelet aggregation data.
  • MODS Multiple organ dysfunction syndrome
  • Figure I tissue injury
  • pancreatitis cancer, peritonitis, surgery, acute lung injury, isehemia-reperfusion. radiation, burns., transfusion reactions, and auto- inflammatory disorders.
  • the organs most affected histologically in MODS are in descending order: the lungs, liver, kidney, heart, gut, brain, pancreas and adrenals, though it i likely that most if not all organs are ultimately affected.
  • disseminated intravascular coagulation is an insidious manifestation of MODS. Endothelial cell death and dysfunction underlies much of the systemic process, resulting in microcireulatory dysfunction and consequent capillary leak, interstitial edema, hemorrhage, and cellular infiltration.
  • Circulating bistones mediate detrimental effects in patients.
  • Eukaryotic genomes are organized in chromatin, a DNA. ⁇ prot.ein complex whose basic repeating unit is the nucleosome.
  • the nuc!eosome consists of DNA wrapped around an octamer of histone proteins (two each ofhistones HS2A, H2B, H3, and H4).
  • severely injured tissues release large quantities of nucleosomes into the circulation, which are further broken down into individual histones.
  • Elevated levels of circulating histories can be derived eithe from damaged and apoptotic cells or from the degradation of neutrophil extracellular traps (NETs), which are structures of extracellular histones and DNA that ensnare and kill bacteria.
  • NETs neutrophil extracellular traps
  • NETs neutrophil extracellular traps
  • circulating histones levels correlate with injury severity, elevated by 4 hours and peaking at about 24 hours after trauma and still detectable at 72 hours.
  • extracellular histones have been detected in bronco alveolar lavage fluids (BALF) from patients with ARDS but not. in BALF from non-A DS patients.
  • BALF bronco alveolar lavage fluids
  • TTLi Transfusion-related acute lung injury
  • N ETs and circulating histone levels are substantially higher in mice and humans with fatal TRALI.
  • nucleosomes or DNA mediates these toxic effects.
  • nucleosomes which have been briefly sonicated or exposed to serum i.e., DNA is degraded and individual histones are released
  • endothelial cell death i lumaii patient serum with histone levels in excess of 50 g ml is toxic to cultured endothelial cells
  • intravenous injection of recombinant histones in mice causes endothelial damage, cytokine elevation, platelet aggregation, and
  • Extracellular histones mediate tissue damage through multiple mechanisms (Figure 2) (Chen, R., et a!., Release and activity of histone in diseases. Cell Death Dis, 2014. 5: p. el 370).
  • Histones disrupt ceil membranes, resulting in Ca "+ influx and subsequent elevations in intracellular Ca 2" concentrations and cell damage. In this way, the tissue injury is amplified through release of additional histones into the circulation.
  • histones bind to and activate TLR2 and TL 4, thereby inducing NF- ⁇ and pro-inflammatory cytokine production.
  • histones have been shown to induce pro-coagulant phenotype in platelets, thereby accelerating blood coagulation and enhancing thrombin generation in a mechanism that involves Toll like receptors (TLR) 2 and 4.
  • TLR Toll like receptors
  • Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response; their potential to amplify tissue injury by killing other cells in addition to their agonistic activity on TLRs provides a rationale to target histones for therapy.
  • approaches currently being pursued in experimental models have marked limitations.
  • TLRs clearly mediate the immune response induced by histones, they have no role in the calcium-medi ted cytotoxicity, negating the use of TLR2/4 neutralizing antibodies.
  • R A aptamers are single-stranded nucleic acids whose binding properties depend on their sequence and structure, Aptamers have high binding affinity and specificity with significant advantages over other biologies, including stability at room temperature, resistance to serum degradation, and minimal immunogenicity. Proposed studies will use the in vitro selection technique SELEX (Systemic Evolution of Ligands by Exponential enrichment) to isolate high affinity RNA aptamers against human histones and test efficacy in human pulmonary microvascular endothelial cells and in animal models of histone-mediated ALL
  • aptamers are analogous to antibodies in their range of target recognition and variety of applications, they possess several ke advantages over their protein counterparts: (I ) they are self-refolding, single-chain, and redox-insensitfve, and unlike proteins do not aggregate. They tolerate pH and temperatures that proteins do not. (2) They are easier and more economical to produce. Their production does not depend on bacteria, ceil cultures or animals.
  • aptamers can easily be chemically modified to yield improved properties and nuclease resistance for plasma stability (6) Their small size leads to a high number of moles of target bound per gram, and transport properties allowing improved tissue penetration (7) They are much more stable at ambient temperature than antibodies yielding a much higher shelf life, and they can tolerate transportation without any special requirements for cooling, eliminating the need for a continuous cold chain.
  • Another important advantage of aptamers is the feasibility of generating cross-species aptamers that enable testing the same reagent in preclinical animal models and in future human clinical trials. Finally, the clinical potential of aptamers is highlighted by the FDA approval of an aptamer-based drug for macular degeneration and by clinical trials that demonstrate the safety and efficacy of systemicaily administered RNA.
  • MODS is described as "the development of potentially reversible physiologic derangement involving two or more organ systems not involved in the disorder that resulted in ICU admission, and arising in the wake of a potentially life-threatening physiologic insult " .
  • ALI acute lung injury
  • ARDS acute respiratory distress syndrome
  • the annual incidence of ALI/ARDS approximates 200,000 cases in the United States, with mortality approaching 40%.
  • ALI acute lung injury
  • ARDS acute respiratory distress syndrome
  • the annual incidence of ALI/ARDS approximates 200,000 cases in the United States, with mortality approaching 40%.
  • the improvement in outcomes following ARDS over the past decade are due to improved strategies of mechanical ventilation and advanced support of other failing organs, as there remains no effective pharmacotherapy to treat patients with this syndrome.
  • ALI/ARDS occurs as a consequence of critical illness of diverse etiologies including initial major trauma, toxic inhalation, burns, near-drowning, radiation, sepsis, and blood transfusion.
  • MODS haemostatic abnormalities ranging from subclinical activation of blood coagulat ion (hypercoagulability) to acute DiC and subsequent consumption of platelets and coagulation proteins causing leeding.
  • BALE Broncho alveolar lavage fluids
  • Transfusion-related acute lung injury is the leading cause of death from transfusion therapy in the US. Neutrophil extracellular traps and circulating histone levels are substantially higher i mice and humans with fatal TRALI.
  • I B and H4 are the major components responsible for this toxicity. Endothelial disruption and consequential coagulation disorders are essential in pathogenesis of sepsis, with 35%of patients with sepsis developing DIC. Changes in the integrity of endothelium are the basis of these severe and mostly fatal complications of sepsis.
  • Ischemia/reperfiisian and drug-mediated tissue injury result in sterile inflammation.
  • Serum histone levels are significantly elevated in animal models with liver, kidney, and brain injury , suggesting an important role of histones in the regulation of sterile inflammation .
  • circulating histones are major mediators of animal death in several liver injury models Including coneanavalin A-triggered liver injury, aeetaminophen-induced.
  • Extracellular histones function as damage- associated molecular patterns (DAMPs) and mediate sterile inflammation and organ damage.
  • DAMPs damage- associated molecular patterns
  • Histone administration in mice increases platelet aggregation and subsequent platelet-dependent thrombin formation, and microvascular thrombosis. Of them, H4 has the strongest impact on platelet activity. Histones also induce a pro-coagulant phenot pe in human platelets, which enhance thrombin generation mid accelerate the blood clotting process. Exogenous histones dose dependently increase plasma thrombin generation in the presence of thrombomodulin.
  • Hi stone release from NETosis has been implicated in a number of autoimmune and auto- inflammatory diseases such as rheumatoid arthritis, systemic lupus, small-vessel vasculitis.
  • Ebola and related viral infections' Ebola and related viral infections'.
  • Ebola virus is very pathogenic in humans and induces an acute hemorrhagic fever that leads to death in about 70% of patients.
  • Viral hemorrhagic fever (VHF) is a severe febrile illnesses caused by enveloped RNA viruses from 4 taxonomie families: Arenaviridae, Bunyaviridae, Filoviridae, and Flaviviridae .
  • vascular damage with capillary leakage ma cause nondependent edema and serous effusions of body cavities such as the pleural and peritoneal spaces. Hemorrhages occur when the patient has thrombocytopenia or severe platelet dysfunction.
  • the extensive multifocal necroses in filoviral infections are probably caused, at least in part, by ischemia associated with fibrin thrombi caused by disseminated intravascular coagulation.
  • the lysis of Ebola-infected cells such as monocytes and macrophages may partially account for cell death.
  • the massive fragmentation of DNA found in the blood and apoptosis was observed early during disease in all fatalities studied, whereas hemorrhagic signs were observed only in some terminal patients.
  • RNA aptamers Development of RNA aptamers to human histories H3 and H4, The goal of these studies is to identify RNA aptamers that selectively bind with high affinity to both human histones H3/H4.
  • RNA oligonucleotides consisting of 51 nucleoli de-long RNA oligonucleotides that include a random region of 20 nucleotides and two constant flanking regions of known sequence (these enable PGR amplification at each selection round).
  • the RNA library is pre-cleared against control proteins BSA and human IgG to remove nonspecific binders and removed by capturing the RNA-protein complexes onto a Centrex nitrocellulose centrifugal filter (high protein/low nucleic acid binding filter). Those RNAs that do not bind BSA or human IgG (flow-through after filtering) are collected and incubated with
  • RNA aptamers that bind to historic 113 are captured onto a second Centrex nitrocellulose centrifugal filter, eluted off the filter with chloroform and extracted.
  • the RNA aptamers are then amplified for the next round of selection with RT-PCR using primers specific to the flanking constan regions of the RNA library followed by in vitro transcription via a T7 promoter included in the S'-PCR primer.
  • the pool of RNA derived from round one is pre-Incubated with BSA and human IgG for the counter-selection step and the flow-through, containing RNAs that do not bind BSA or human IgG, incubated with commercially available recombinant human histone f 14. ' Those RNAs that bind histone H4 are eluted and amplified as described above. Selection alternates between human histone H3 and H4 for several rounds in order to isolate aptamers that bind to both of these proteins. High affinity aptamers are enriched by modifying the selection conditions (e.g.
  • RNA: hi stone ratio during the positive selection step and reducing the incubation time of aptamers with histones H3/H4) The progress of the selection is assessed by determining the sequence diversity of the random regions from round to round by high-throughput sequencing (HTS) and identifying sequence and structure families using bioinformatics analyses. Progress of the selection is assessed by carrying out standard in vitro binding assays (e.g. double-filte nitrocellulose filter binding assays or surface plasmon resonance measurements) with the pools of RNA from each round.
  • standard in vitro binding assays e.g. double-filte nitrocellulose filter binding assays or surface plasmon resonance measurements
  • aptamers identified from the sequence analysis are screened for ( 1) specificity of binding and for (2) ability to inhibit histone activity.
  • In vitro filter binding assays are performed with the top 10- 15 sequences from the HTS and bioinformatics analysis. Radiolabeled aptamers re incubated with varying amounts of control proteins (BSA and human IgG) or histone H3 or H4 proteins for 5 nitn prior to loading onto a dot-blot apparatus. Aptamers bound to histone proteins are retained onto the nitrocellulose filter while unbound aptamers will be retained onto the nylon filter.
  • BSA and human IgG control proteins
  • histone H3 or H4 proteins for 5 nitn prior to loading onto a dot-blot apparatus.
  • Membrane filters are quantified using a phospho imager screen and a corrected fraction bound will be determined for each aptamer. Binding specificity is assessed by determining background binding of each aptamer to control proteins (BSA and IgG). A more extensive quantitative determination of binding affinities via surface plasmon resonance is reserved for the 5 aptamers that demonstrate the greatest affinity and specific binding in the filter binding assays.
  • biotin is coupled to the aptamers during chemical synthesis then immobilized on streptavidin-coupled chips using standard protocols. Recombinant human h stone H3 and H4 proteins are injected over the sensor surface for 5 min (association and dissociation time). Various concentrations of histone proteins are injected by serially diluting samples.
  • Concentrations are adjusted based on the binding signal response to obtain optimal affinity determinations.
  • the selectivity studies are carried out by injecting comparable concentrations of several control proteins (i.e. BSA and human IgG) over the immobilized aptamers.
  • the dissociation constant. (Kn) for each aptamer is calculated by global fitting of four concentrations of histone proteins, assuming a constant density of aptamers on the surface of the chip.
  • a 1 :1 binding mode with mass transfer fitting is used to obtain the kinetic data.
  • BSA and human IgG measurements are aligned to histone I B and 114 data for the nonspecific analysis.
  • Human patient serum with histone levels in excess of 50 ,ug/ml is toxic to EC2; therefore this concentration is used in vitro.
  • Dose response curves for the aptamers are determined in vitro and define the concentration used in in vivo studies.
  • Endothelial cell culture Endothelial monolayers are treated with histones (SO pg/ml) with and without one of three aptamers (negative binding, 2 histone binding) at varying aptamer concentrations, based on the K D for each aptamer determined above (typical range is liim-lOOnm). Cells also receive aptamers without histones as a control. The following measurements are made: Measurement of calcium influx. Intracellular calcium concentration is measured using established protocols with fura 2-AM as the fluorescent probe.
  • Detection of cell toxicity Live, apoptotic, and dead cells are determined by flow cytometry following staining with annexin-V and propidium iodide.
  • Platelet activation Blood Is collected from human volunteers, platelet-rich plasma prepared, and platelets isolated. Using the histone and aptaraer treatment protocol described above, studies are performed in a collagen- coated flow chamber perfused with washed human platelets. The surface area covered by platelet aggregates is quantified. Shear stress conditions are adjusted to replicate the values for arterial (-20 dynes/cm") or venous shear stress ( ⁇ 3 dynes/cm'). In separate studies, platelet surface marker expression (activated a2b 3 and P-se!ectin) are detected by fluorescent antibodies followed by flow cytometry.
  • Animal model The best evidence of whether A LI has occurred in an animal is provided by a measurement of the cellular response (such as neutrophil counts in the BALF), a measurement of the integrity of the alveolar capillary barrier (such as movement of high molecular weight proteins from the serum into the airspaces) and histological images showing lung injury.
  • Histones are injected into the tail vein of C57BL/6 mice (50 nig/kg body weight).
  • the two aptamers identified in the in vitro experiments- tha are most effective and have greatest binding are tested.
  • Animals are- pretreated with a control aptamer or one of the two histone aptamers (1 mg/kg or 5 mg kg, IV) 10 minutes prior io histone administration.
  • aptamers are administered at various times after delivery of the histones (5, 15, and 30 minutes) to examine the ability of the aptamer to prevent injury after histone release.
  • Noninvasive determination of respiratory function is continuously monitored using a pulse oximeter. After 3 hours (or at the time of death if occurs prior to 3 hours) the mice are killed and the following performed:
  • TLR activation Blood is collected and serum isolated and stored at -80°C.
  • Cytokines/chemokines are all quantified using a customized multiplex magnetic bead panel kit.
  • Alveolar permeability and inflammation Tracheas are isolated and cannulated and the lungs flushed with sterile PBS. Cytospins of BALF cells are stained with modified Wright- Gierasa. Alveolar permeability is assessed by quantitative detection of mouse albumin in BALF by ELISA.
  • Lung histology Lungs are fixed in 4% (w/v) paraformaldehyde for 24 h and embedded and sectioned. Sections are stained with hematoxylin and eosin (H&E) and Mallory's phosphotungstic acid-hematoxylin staining for fibrin then examined by a pathologist for evidence of neutrophils in alveolar spaces, capillary congestion, intovalveolar hemorrhage, fibrin deposits and thrombi.
  • H&E hematoxylin and eosin
  • Mallory's phosphotungstic acid-hematoxylin staining for fibrin then examined by a pathologist for evidence of neutrophils in alveolar spaces, capillary congestion, intovalveolar hemorrhage, fibrin deposits and thrombi.
  • Statistical analysis All data is expressed as mean ⁇ SEM. Statistical analysis is carried out using computer software SYSTAT. Results are analyzed using paired and unpaired t-tests for comparisons
  • mice The ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines have been reviewed and are followed for reporting of research findings. Studies are performed in male and female C57BL/6 mice (-10 wks of age), using the minimum number of mice required to address the descri bed protocols. For the animal studies, a power analysis assumin a 25% difference between the control and treatment groups with a standard deviation of 20% for both groups, an alpha error level of 5% (two sided) and a beta error level of 50% indicates a sample size of 8 mice for each study gro p and for each protocol. Based on these estimates it is estimated that a total of approximately 192 mice (3 aptamers x 4 time points x 2 doses) are needed to complete the study. Prior to collection of tissues, the mice are euthanized by a barbiturate overdose.
  • Humans Healthy individuals (>18 years of age, 50% males) who are not pregnant are recruited from laboratory personnel to donate venous blood for the isolation of platelets. The population recruited reflects the race and etlinic composition of the community. The potential risks of phlebotomy are limited to local pain and local hematoma. Multiple experimental groups are studied from a single 10 ml blood draw.
  • the present technology uses nucleic acid aptamer constructs that have been created to specifically inhibit the human H3 and H4 hi. stones, in certain embodiments, the constructs are chemically modified (2'-fluoropyridines) RNA constructs making them nuclease resistant.
  • RNA aptamers are RNA
  • RNA aptamers have the following characteristics: (1) RNA aptamer libraries have a higher structural complexity as compared to DNA aptamer libraries (most DNA aptamers form predictable and similar G-quadrupkx structures), thereby- increasing the likelihood of identifyin aptamers with the desired properties; and (2) RNA chemistries to increase serum stability and safety have been more extensivel evaluated for in vivo applications.
  • DNA aptamers are used.
  • Aptamers are single stranded oligonucleotides that can natural !y fold into different 3- dimensional structures, which have the capability of binding specifically to biosurfaces, a target compound or a moiety.
  • formational change refers to the process by which a nucleic acid, such as an. aptamer, adopts a different secondary or tertiary structure.
  • fold may be substituted for conformational change,
  • Aptamers have advantages over more traditional affinity molecules such as antibodies in that they are very " stable, can be easily synthesized, and can be chemically manipulated with relative ease. Aptamer synthesis is potentially far cheaper and reproducible than antibody-based diagnostic tests. Aptamers are produced by solid phase chemical synthesis, an accurate and reproducible process with consistency among production batches. An aptamer can be produced in large quantities b polymerase chain reaction .(PGR) and once the sequence is known, can be assembled from individual naturally occurring nucleotides and/or synthetic nucleotides, Aptamers are stable to long-term storage at room temperature, and, if denatured., aptamers can easily be renatured, a feature not shared by antibodies. Furthermore, aptamers have the potential to measure concentrations of ligand in orders of magnitude lower (parts per trillion, or even quadrillion) than those antibody-based diagnostic tests. These characteristics of aptamers make them attractive for diagnostic applications.
  • Aptamers are typically oligonucleotides that may be single stranded
  • modified nucleotides encompasses nucleotides with a eovalent!y modified base and/or sugar.
  • modified nucleotides include nucleotide having sugars which are covalently attached to low molecular weight organic groups other than a hydroxy! group at the 3' position and other than a phosphate group at the 5' position.
  • modified nucleotides may also include 2' substituted sugars such as 2'-0-methyl ⁇ ; 2-O-alkyl; 2-O-allyl; 2 -S-alkyI; 2'-S-allyl; 2 -fluoro-; 2.'-halo or 2-azido-ribose, carbocyclic sugar analogues a-anomeric sugars; epimeric sugars such as arabinose, xyloses or lyxoses, pyranose sugars, furanose sugars, and sedoheptulose.
  • 2' substituted sugars such as 2'-0-methyl ⁇ ; 2-O-alkyl; 2-O-allyl; 2 -S-alkyI; 2'-S-allyl; 2 -fluoro-; 2.'-halo or 2-azido-ribose, carbocyclic sugar analogues a-anomeric sugars; epimeric sugars such as arabinose, xyloses or lyxoses,
  • Modified nucleotides include, by example and not by way of limitation, alkylated purines and/or pyrimidines; acy!ated purines and/or pyrimidines; or other heterocycles. These classes of pyrimidines and purines are known in the art and include, pseudoisocytosine; N4, N4-ethatiocytosine; 8-hydroxy-N6-methyladenine; 4- acetyJcytosine, 5 ⁇ (carbox.yhydroxylmethyl) uracil; 5-fluorouracil.; 5-bromouracil; 5 ⁇ carboxymethylaminomethyl-2-thiouracil; 5-carboxymethylaminomethyl uracil;
  • uracil-5-oxyacetic acid methyl ester psueouraciJ; 2-thiocytosine; 5-methyl-2 thiouraeih 2- thiouracil; 4-thio uracil; 5-methyluracil; N-uracil-5-oxyacetic acid methylesier; uracil 5- oxyacetic acid; queosine; 2 hiocytosme; 5 -propyl uracil; 5-propyl.cytositie; 5-ethyluracil; 5- ethylcytosine; 5-butyluracil; 5-pentyluracil; 5-pentyleytosine; and 2,6,-diaminopurine;
  • aptame modifications include: (a) Nucleotides modified by replacing the 2' position with either a fiuoro- (F),. amino- (NH 2 ) or O-roethyl ⁇ OCH 3 ) group for enhanced nuclease resistance. These modified nucleotides are introduced either chemically or enzymatically. (b) Bridging phosphorothioates incorporated enzymatically. (c) End caps that involve reversing the polarity of the chain incorporated during chemical synthesis, (d) Linkers inserted at the 5 '-ends of aptarners by either chemical or enzymatic means to provide handles for conjugation or to alter pharmacokinetic properties. Keefe et al, Aptarners as therapeutics, Nat Rev Drug Discov. 2010 Jui;9(7):537-50.
  • modification of the '-position of the ribose indirectly improves nuclease resistance of the mternucleotide phosphate bond and at the same time increases duplex stability (T m ), and also provides protection from immune activation.
  • 2'-0- methyl RNA (2'OMe) is a naturally occurring RNA variant found in mammalian ribosomal RNAs and transfer RNAs. it is nontoxic and can be placed within either the S or AS strands of a si RNA. Heavy modification with 2'OMe RNA can reduce potency or completely inactivate a siRNA; alternating 2'OMe with RNA bases generally retains siRNA function while conferring significant nuclease stabilization. 2'OMe RNA can be combined with other 2 '-modifications which are not naturally occurring bases with good results. Behlke, Chemical Modification of ' siRNAs for in Vivo Use, Oligonucleotides 18:305-320 (2008).
  • the 2'-iluoro (2'-F) modification is compatible with siRNA function and also helps stabilize the duplex against nuclease degradation. Incorporatio of 2'-F at pyrirnidine positions maintains siRNA activity in vitro and in vivo. The 2 -F modification is even tolerated at the site of Ago2 cleavage.
  • the combined use of 2 -F pyrimidines with 2'OMe purines can results in RNA duplexes with extreme stability in serum and improved in vivo performance.
  • the 2'-0-(2-methoxyethyI) RNA (MOE) modification has been extensively used in anti sense oligonucleotides and confers significant nuclease stability to an .
  • oligonucleotide as well as increases T m .
  • 2'- OE residues can be incorporated into siRNAs much like 2'OMe or 2' ⁇ F, however this modification is not generally available for use.
  • the 2'- fluoro- -D-arabinonucieotide (FANA) modification has also shown promise in antisense oligonucleotide applications and can also be placed in siRNAs, Substitution of FANA for RNA in the entire S-strand confers significant stabilization to nucleases while maintaining functional potency of the duplex; however, the AS-strand is less tolerant of the FANA modification.
  • Locked nucleic acids contain a methylene bridge which connects the 2'-0 with the 4'-C of the ribose.
  • the methylene bridge 'locks "the sugar in the 3'-endo conformation, providing both a significant increase in T m as well as nuclease resistance.
  • Extensive modification of a siRNA with LNA bases generally results in decreased activity (even more so than 2'OMe); however, siRNAs with limited incorporation retain functionalit and offer significant nuclease stabilization.
  • the aptamers of the invention are synthesized using conventional phosphodiester linked nucleotides and synthesized usin standard solid or solution phase synthesis techniques which are known in the art.
  • Linkages between nucleotides may use alternative linking molecules.
  • nucleic acid molecule of not more than 90 nucleotides in length comprising an aptamer, wherein the aptamer specifically targets extracelluiar histones.
  • the aptamer portion is specific for human histones H3 and/or H4.
  • the aptamer comprises at least 90% identity to an RNA sequence listed in Table 1. In certain embodiments, the aptamer comprises at least 95% identity to an RNA sequence listed in Table I . In certain embodiments, the aptamer comprises 100% identity to an RNA sequence listed in Table 1.
  • the aptamer specifically targets human H3 and 114, In certain embodiments, the aptamer comprises at least 90% identity to KU1 , KIJ2 or U3. In certain embodiments, the aptamer comprises at least 95 identity to KU1 , KU2 or KU3. In certain embodiments, the aptamer has 100% identity to KU 1 , KU2 or KU3.
  • the aptamer specifically targets human H3.
  • the e aptamer comprises at least 90% identity to IJ7, KUS or U9.
  • the aptamer comprises at least 95% identity to K.U7, KU8 or TJ9,
  • the aptamer has 100% identity to U7, KU or KU9.
  • the aptamer specifically targets human H4.
  • the aptamer comprises at least 90% identity to KU4, U5 or KU6.
  • the aptamer comprises at least 95% identity to U4, KU5 or U6.
  • the aptamer has 100% identity to KU4, KU5 or KU6.
  • additional modifications are made to the aptamer portion. Additional modifications to the aptamer portion include 2'0-methyi modification of the pyrimidines. In other embodiments, all of the nucleotides in the aptamer are 2 ' 0-meth l modified. Alternatively, the pyrimidines, or all the nucleotides, may be modified with 2'fluoros (both pyrimidines and purines). Additional modifications to the nucleotides in the aptamer include large molecular weight conjugates like PEGy!ation, lipid-based
  • modifications e.g., cholesterol
  • nanoparticles e.g., PET or chitosan
  • the aptamers are linked to other molecules in order to improve clearance (pharmacokinetics and pharmacodynamics).
  • the other molecules include PEG, cholesterol or other lipid, nanoparticles.
  • the aptamers of the present invention can be operably linked to one or more small molecule entities, in certain embodiments, the entity is a fluorescen tag, affinity tag, a protein, a solid substrate, a cell surface, or a cellular component.
  • the cellular component is a cell wall or ceil membrane.
  • the solid substrate is a component of si lica, cellulose, cellulose acetate, nitrocellulose, nylon, polyester, polyethersu!fone, polyolefm, or polyvinylidene fluoride, or combinations thereof.
  • the solid substrate is a stent or other medical device, filter, magnetic bead, metal oxide, latex particle, microliter plates, polystyrene bead, or CD-ROM.
  • the aptamer i linked to the entity by means of a linker.
  • the linker is a binding pair
  • the "binding pair” refers to two molecules which interact with each other through any of a variety of molecular forces including, for example, ionic, covalent, hydrophobic, van der Waals, and hydrogen bonding, so that the pair have the property of binding specifically to each other.
  • Speci fic binding means that the binding pair members exhibit binding to each other under conditions where they do not bind to another molecule.
  • binding pairs are biotin-avidin, hormone-receptor, receptor-ligand, enzyme-substrate, IgG-protein A, antigen-antibody, and the like.
  • a first member of the binding pair comprises avid in or streptavidin and a second member of the binding pair comprises biotin.
  • the aptamer is linked to the entity by means of a covalent bond.
  • the entity may additionally or alternatively, be a detection means.
  • a number of "molecular beacons" (such as fluorescence compounds) can be attached to aptamers to provide a means for signaling the presence of and quantifying a target chemical or biological agerit.
  • Other exemplary detection labels that could be attached to the aptamers include biotin, any fluorescent dye or tracer, amine modification, horseradish peroxidase, alkaline phosphatase, etc.
  • the aptamer is operably linked to a detection means and to a solid substrate.
  • the aptamer may be linked to a fluorescent dye and to a magnetic bead.
  • the small molecules are biologic or pharmacologic agents that can inhibit H3 and/or H4 liistones
  • Chemistries thai can be used to link molecules to the aptamer are known in the art, such as disulfide linkages, amino linkages, covalent linkages, etc. Additional linkages and modifications can be found on the world-wide-web at
  • non-antibody therapeutic agents targeted against either histones or downstream effectors of a histone mediated pathway may also be utilized in combination with anti-histone antibodies or fragments thereof, administered either before, simultaneousl with, or following administration of one or more anti-histone antibodies or fragments thereof.
  • Various therapeutic agents of use in treating histone associated disease states are known in the art, such as activated protein C (APC), thrombomodulin, a peptide fragment of histone i f 1 , ⁇ 2 ⁇ , H2B, ⁇ 13 or H4, granzyme A, granzyrne B, plasmin, Factor 7-activating protease, heparin, and any such known agent may be utilized in combination with the subject anti-histone antibodies or antibody fragments.
  • a human histone H4 peptide may comprise residues 50-67 or 40-78 of human H4 (see, e.g., U.S. Pub!. No. 200901 1 7099).
  • the therapeutic agent is selected from the group consisting of an antibody, an antibody fragment, an immune conjugate, a radionuclide, an
  • an anti-angiogenic agent a pro-apopto ie agent
  • a cytokine a chemokine
  • a drug a toxin, a hormone, an siR A, a cytokine, a chemokine, a coagulation inhibitor, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony stimulating factor, an interferon, erythropoietin, thrombopoietin, an enzyme, recombinant human thrombomodulin and activated human protein C.
  • the antibody, antibody fragment or immune conjugate binds to an antigen selected from the group consisting of histone H2B, histone H3, historic H4.
  • a proinflammatory effector of the innate immune system a proinilammatory effector cytokine, a proinflammatory effector chemokine, a target specifically associated with infectious disease, acute respiratory distress syndrome, septicemia, septic shock, GVHD, transplant rejection, atherosclerosis, asthma, granulomatous disease, a neuropathy, cachexia, a coagulopathy, acne, giant cell arteritis or myocardial ischemia, TNF-a, MIP, CD74, HLA- DR, IL ⁇ J . !
  • the drug is selected from the group consisting of 5- fmoro uracil, aplidin, azaribine, anastrozole, anthracyclines, bendamustine, bleomycin, bortezomib, bryo statin- 1 , busulfan, calicheamycin, camptothecin, carboplatin, 10- hydroxycamptothecin, carmustine, celebrex, chlorambucil, cisp latin (CDDP), Co
  • cyclophosphamide cytarabine, daearhazine, docetaxel, dactinomycin, daunorubicin, doxorubicin, 2-pyrrolinodoxorubicine (2P-DOX), cyano-morpholino doxorubicin, doxorubicin glucuroni.de, epirubicin g!ucuronide, esiramustine, epipodophyilotoxin, estrogen receptor binding agents, etoposide (VP 16), etoposide g!ucuronide, etoposide phosphate, tloxuridine (FUdR), 3',5'-0-dioleoyI ⁇ FudR (FUdR-dO), fJudarabine, flutamide, farnesyi- protein transferase inhibitors, gemcitabine, hydroxyurea, idaruhicin, ifosfamide, L- asparaginase, lenolidamide,
  • mercaptopurine 6-mercaptopurine, methotrexate, mitoxantrone, mithramycin, mitomycin, mitotane, navelbine, nitrosourea, plieomycin, procarbazine, paeiitaxel, peniostatin, PSi-341, raloxifene, semustine, streptozocin, tamoxifen, taxol, temazolomide (an aqueous form of DTIG), transplatinum, thalidomide, ihioguanuie, thiotepa, teniposicle, topotecan, uracil mustard, vinoreibine, vinblastine, vincristine, a vinca alkaloid, a tyrophostin, canertinib, dasatinib, erlotinib.
  • the toxin is selected from the group consisting of ricin. abrin. alpha toxin, saporin, ribonuclease (RNase). e.g., onconase, DNase I, Staphylococcal enterotoxin-A. poke weed antiviral protein, gelomn, diphtheria toxin, Pseudamonas exotoxin, and Pseudomonas endotoxin.
  • RNase ribonuclease
  • the immunomodulatory is selected from the group consisting of a cytokine, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony stimulating factor (CSF), an interleukin (IL), erythropoietin, thrombopoieiin, tumor necrosis factor (TNF), granulocyie-cofony stimulating factor (G-CS.F), granulocyte, macrophage- colony stimulating factor (G -CSF), interferon-a, interferon- ⁇ , interferon- ⁇ , interferon-!.
  • TGF-a, TGF- ⁇ , interleukin- 1 (IL-1), IL-la are examples of TGF-a, TGF- ⁇ , interleukin- 1 (IL-1), IL-la,.
  • the cytokine is selected from the group consisting of human growth honnone, N-meihionyl human growth hormone, bovine growth hormone, parathyroid hormone, thyroxine, insulin, proinsulin, relaxin, prorelaxin.
  • FSH follicle stimulating honnone
  • TSH thyroid stimulating hormone
  • LH luteinizing hormone
  • hepatic growth factor prostaglandin
  • fibroblast growth factor prolactin
  • placental lactogen OR protein
  • tumor necrosis factor-a tumor necrosis factor- ⁇
  • mulierian-inhibiting substance mouse
  • gonadotropm-associated peptide gonadotropm-associated peptide, inhibin, activin, vascular endothelial growth factor, integrin, thrombopoieiin (TPO), NGF-fl platelet-growth, factor, TGF-a, TGF- ⁇ , insulin-like growth factor-], insulin-like growth factor-II, erythropoietin (EPO), osteoinductive factors, interferon-a, interferon- ⁇ , interferon - ⁇ .
  • M-CSF macrophage-CSF
  • IL-1 IL-1 , IL-l a, 11 -2, IL-3, IL-4, IL-5, IL-6, IL-7, 1L-8, IL-9, IL-10, IL-1 1 , IL-12, f i - 13.
  • IL-14 IL- 1 5, IL-16, ii - 17, IL- 18, l.L-21 , IL-25, LIF, FLT-3, angiostatin, thrombospondin, endostatin, tumor necrosis factor and lymphotoxin.
  • the radionuclide is selected from the group consisting of m In, 1 H At, m Lu, 2n Bi, 212 Bi, 2!3 Bi, 21 'At, 62 Cu, 67 Cu, 9 % 125 I, m l, i r % 32 P, 3 ⁇ 4 47 Sc, u , Ag, 67 Ga, i i3 Sm, ,6, Tb, ,53 Dy, m Oy, 61 Ho, !66 I!o, !86 Re, m Re, i3 ⁇ 4) Re, 2i, Pb, m Pb, 223 Ra, 2 5 Ac, 77 As, 3 ⁇ 4r, "Mo, l05 Ru, 549 Pm, ,69 Er, m lr, 53 ⁇ 4 Co, 80m Br, 99m Tc, 103m Rh, 10 Pt !
  • the method involves the amplification of selected RNAs.
  • “Amplifying'' utilizes methods such as the polymerase chain reaction (PGR), ligation amplification (or ligase chain reaction, LCR), strand displacement amplification, nucleic acid sequence-based amplification, and amplification methods based on the use of Q- beta replicase. These methods are well known and widely practiced in the art, Reagents and hardware for conducting PCR are commercially available.
  • PGR polymerase chain reaction
  • LCR ligase chain reaction
  • strand displacement amplification strand displacement amplification
  • nucleic acid sequence-based amplification and amplification methods based on the use of Q- beta replicase.
  • Reagents and hardware for conducting PCR are commercially available.
  • at leasl one type of aptamer is immobilized on a solid surface.
  • the amplification may be carried out by any means known to the art.
  • suitable amplification techniques include, but are not limited to, polymerase chain reaction (including, for RNA amplification, reverse- transcriptase polymerase chain reaction), ligase chain reaction, strand displacement amplification, transcription-based amplification, self-sustained sequence replication (or '"3SR ,; ), the Qp replicase system, nucleic acid sequence-based amplification (or "NASBA"), the repair chain reaction (or "RCR”), and boomerang DNA amplification (o "BDA”).
  • polymerase chain reaction including, for RNA amplification, reverse- transcriptase polymerase chain reaction
  • ligase chain reaction strand displacement amplification
  • transcription-based amplification transcription-based amplification
  • self-sustained sequence replication or '"3SR ,;
  • Qp replicase system or "NASBA”
  • RCR repair chain reaction
  • BDA boomerang DNA amplification
  • bases incorporated into the amplification product may be natural or modified bases (modified before or after amplification), and the bases may be selected to optimize subsequent electrochemical detection steps.
  • PCR Polymerase chain reaction
  • a nucleic acid sample e.g., in the presence of a heat stable DNA polymerase
  • one oligonucleotide primer for each strand of the specific sequence to be detected under hybridizing conditions so that an extension product of each primer is synthesized that is complementary to each nucleic acid strand, with the primers sufficiently complementary to each strand of fhe specific sequence to hybridize therewith so that the extension product, synthesized from each primer, when it is separated from its complement, can serve as a template for synthesis of the extension product of the other primer, and then treating the sample under denaturing conditions to separate the primer extension products from their templates if the sequence or sequences to be detected are present.
  • Detection of the amplified sequence may be can ied out by adding to the reaction product an oligonucleotide probe capable of hybridizing to the reaction product (e.g.,. an oligonucleotide probe of the present invention), the probe carrying a detectable label, and then detecting the label in accordance with known techniques.
  • ampiification may be carried out by initial conversion to DNA by reverse transcriptase in accordance with known techniques.
  • Strand displacement ampiification may be carried out in accordance with known techniques. For example. SDA may be earned out with a single amplification primer or a pair of amplification primers, with exponential amplification, being achieved with the latter.
  • SDA amplification primers comprise, in the 5' to 3' direction, a flanking , sequence (the DNA sequence of which is noncritical ), a restriction site for the restriction enzyme employed in the reaction, and an oligonucleotide sequence (e.g., an oligonucleotide probe of the present invention) that hybridizes to the target sequence to be amplified and/or detected.
  • flanking sequence which serves t facilitate binding of the restriction enzyme to the recognition site and provides a DNA polymerase priming site after the restriction site has been nicked, is about 15 to 20 nucleotides in length in one embodiment.
  • the restriction site is functional in the SDA reaction.
  • the oligonucleotide probe portion is about 13 to 15 nucleotides in length in one embodiment of the invention.
  • Ligase chain reaction is also carried out in accordance with known techniques, in general, the reaction is carried out with two pairs of oligonucleotide probes: one pair binds to one strand of the sequence to be detected; the other pair binds to the other strand of the sequence to be detected. Each pair together completely overlaps the strand to which it corresponds.
  • the reaction is carried out by, first, denaturing (e.g., separating) the strands of the sequence to be detected, then reacting the strands wi th the two pairs of oligonucleotide probes in the presence of a heat stable ligase so that each pair of oligonucleotide probes is ligated together, then separating the reaction product, and then cyclically repeating the process until the sequence has been amplified t the desired degree. Detection may then be carried out in like manner as described above with respect to PCR.
  • Diagnostic techniques that are useful in the methods of the invention include, but are not limited to direct DNA sequencing, pulsed-field gel electrophoresis (PFGE) analysis, allele-specific oligonucleotide (A SO), dot blot analysis and denaturing gradient gel electrophoresis, and are well known to the artisan.
  • PFGE pulsed-field gel electrophoresis
  • a SO allele-specific oligonucleotide
  • dot blot analysis and denaturing gradient gel electrophoresis
  • the sample may be contacted with the aptamer in any suitable manner known to those skilled in the art.
  • the sample may be solubilized in solution, and contacted with the aptamer by solubilizing the aptamer in solution with the sample under conditions that permit binding. Suitable conditions are well known to those skilled in the art.
  • the sample may be solubilized in solution with the aptamer immobilized on a solid support, whereby the sample may be contacted with the a tamer by immersing the solid support, having the aptamer immobilized thereon in the solution containing the sample.
  • “Synthetic” aptamers are those prepared by chemical synthesis. The aptamers may also be produced by recombinant nucleic acid methods. "Recombinant nucleic molecule” is a combination of nucleic sequences that are joined together using recombinant nucleic technology and procedures used to join together nucleic sequences known in the art.
  • a “therapeutic agent' is an atom, molecule, or compound that is useful in the treatment of a disease.
  • therapeutic agents include antibodies, antibody fragments, peptides, drugs, toxins, enzymes, nucleases, hormones, immune modulators, anti sense oligonucleotides, small interfering RNA (siRNA), chelators, boron compounds, photoactive agents, dyes,, and radioisotopes.
  • An “antibody” as used herein refers to a full- length (i.e., naturally occurring or formed by normal immunoglobulin gene fragment recombinatorial processes) immunoglobulin molecule (e.g., an IgG antibody).
  • an “antibody” includes monoclonal, polyclonal, bispecific, multispecific. murine, chimeric, humanized and human antibodies.
  • An "antibod fragment ' ' is a portion of an intact antibody such as F(ab')2, F(ab)2, Fab', Fab, Fv, st, scFv, dAb and the like. Regardless of structure, an antibody fragment binds with the same antigen that is recognized by the full length antibody.
  • antibody fragments include isolated fragments consisting of the variable regions, such as the "Fv” fragments consisting of the variable regions of the heavy and light chains or recombinant single chain polypeptide molecules in which light and heavy variable regions are connected by a peptide linker ("scFv proteins " ').
  • scFv proteins a peptide linker
  • Single chain antibodies often abbreviated as “scFv” consist of a polypeptide chain that comprises both a VH and a V ; domain which interact to form an. antigen-binding site.
  • the VH andVi, domains are usually linked by a peptide of 1 to 25 amino acid residues.
  • Antibody fragments also include diabodies, triabodies and single domain antibodies (dAb), Fragments of antibodies that do not bind to the same antigen as the intact antibody, such as the Fc fragment, are not included within the scope of an "antibody fragment” as used herein.
  • the antibody is an anri- histone antibody (see., e.g., US 2014/0234209, which is incorporated by reference herein).
  • a "diagnostic agent * ' is an atom, molecule, or compound that is useful in diagnosing a disease.
  • useful diagnostic agents include, but are not limited to, radioisotopes, dyes (such as with the biotin-streptavidm complex), contras agents, fluorescent compounds or molecules, and enhancing agents (e.g., paramagnetic ions) for magnetic resonance imaging (MR1).
  • An anii-histone aptamer, antibody or antibody fragment, or a composition described herein, is said to be admi istered in a "therapeutically effective amount ' ' if the amount administered is physiologically significant.
  • An agent is physiologically significant if its presence results in a detectable change in the physiology of a recipient subject.
  • an aptamer, antibody or antibody fragment preparation is physiologically significant if its presence invokes an antitumor response or mitigates the signs and symptoms of an autoimmune disease state.
  • a physiologically significant effect could also be the evocation of a humoral and/or cellular immune response in the recipient subject leading to growth inhibition or death of target cells.
  • chimeric refers to a gene or DNA that contains 1 ) DMA sequences, including regulatory and coding sequences that are not found together in nature or 2) sequences encoding parts of proteins not naturally adjoined, or 3) parts of promoters that are hot naturally adjoined. Accordingly, a chimeric gene may include regulatory sequences and coding sequences that are derived from different sources, or include regulatory sequences and coding sequences derived from the same source, but arranged in a manner different from that found in nature.
  • nucleic acid and “polynucleotide” refers to
  • the term encompasses nucleic acids containing known analogs of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring, nucleotides.
  • a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g , -degenerate codon substitutions) and. complementary sequences as well as the sequence explicitly indicated.
  • degenerate codon substitutions may be achieved by generating sequences in which the third, position of one or more selected f or all) codons is substituted with mixed- base and/or deoxyinosine residues.
  • nucleic acid fragment is a portion of a given nucleic acid molecule.
  • nucleotide sequence refers to a polymer of DNA or RNA which can be single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases capable of incorporation into DNA or RNA polymers.
  • nucleic acid may also be used interchangeably with gene, cDNA, DNA and RNA encoded by a gene, e.g., genomic DNA, and even synthetic DNA sequences.
  • sequences hat include any of the known base analogs of DNA and RNA.
  • variants include those sequences that, because of the degeneracy of the genetic code, encode the identical amino acid sequence of the native protein. Nciturally occurring allelic variants such as these can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PGR) and hybridization techniques. Variant nucleotide sequences also include syntheticall derived nucleotide sequences, such as those generated, for example, by using site -directed mutagenesis that encode the native protein, as well as those that encode a polypeptide having amino acid substitutions.
  • nucieotidc sequence variants of the invention will have in at least one embodiment 40%. 50%, 60%, to 70%, e.g., 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%. to 79%. generally at least 80%, e.g. , 81%-84%, at least 85%, e.g., 86%, 87%. 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98%, sequence identity to the native (endogenous) nucleotide sequence.
  • sequence identity or 'identity in the context of two nucleic acid sequences makes re ference to a specified percentage of residues in the tw o sequences that are the same when aligned by sequence comparison algorithms or by visual inspection.
  • percentage of sequence identity means the value determined by comparing two optimally aligned sequences, wherein the portion of the polynucleotide sequence may comprise additions or deletions ⁇ i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity.
  • substantially identical of polynucleotide sequences means that a
  • polynucleotide comprises a sequence that has at least 70%, 71%, 7.2%, 73%, 74%, 75%, 76%. 77%, 78% or 79%; at least 80%,, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, or 89%; at least 90%, 91%, 92%, 93%, or 94%; or even at least 95%, 96%, 97%, 98%, or 99% sequence identity,: compared to a reference sequence using one of the alignment programs described using standard parameters.
  • nucleotide sequences are substantially identical if two molecules hybridize to each other under stringent conditions (see below).
  • stringent conditions are selected to be about 5°C lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH.
  • T m thermal melting point
  • stringent conditions encompass temperatures in the range of about PC to about 20°C, depending upon the desired degree of stringency as otherwise qualified herein.
  • Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the polypeptides they encode are substantially identical. This may occur, e.g., when a copy of a nucleic acid is created using the maximum, codon degeneracy permitted by the genetic code.
  • hybridizing specifically to refers to tine binding, duplexing, or hybridizing of a molecule only to a particular nucleotide sequence under stringent conditions when that sequence is present in a complex mixture (e.g., total cellular) DNA or RN A.
  • Bod(s) substantially refers to complementary hybridization between a probe nucleic acid and a target nucleic acid and embraces minor mismatches that can be accommodated b reducing the stringency of the hybridization media to achieve the desired detection of the target nucleic acid sequence.
  • the T m can be approximated from, the equation of Meinkoth and Wahh T ra 81.5°C + 16.6 (log M) +0.41 (%GC) - 0.61 (% form) - 500/1,.
  • M is the molarity of monovalent cations
  • %GC is the percentage of guanosine- and cytosine nucleotides in the DN A
  • % form is the percentage of formamide in the hybridization solution
  • L is the length of the hybrid in base pairs.
  • T ra is reduced by about 1 °C for each 1 % of mismatching; thus, T m , hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity.
  • the T,ILE can be decreased 1 G°C.
  • stringent conditions are selected to be about 5°C lower than the thermal melting point (T m ) for the specific sequence and its complement at a defined ionic strength and pi L
  • severely stringent conditions can utilize a hybridization and/or wash at 1 , 2. 3, or 4°C lower than the thermal melting point (T m );
  • moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9, or 10°C lower than the thermal melting point (T m ); low stringency conditions can utilize a
  • hybridization and/or wash at 1 1, 12, 13, 14, 15, or 20°C lower than the thermal melting point (Tm)-
  • Tm thermal melting point
  • T TO thermal melting point
  • An example of highly stringent wash conditions is 0. 5 M NaCl at 72°C for about 15 minutes.
  • a example of stringent wash conditions is a 0.2X SSC wash at 65 °C for 15 minutes.
  • a high stringency wash is preceded by a low stringency wash to remove background probe signal.
  • An example medium stringency wash for a duplex of, e.g, more than 100 nucleotides is IX SSC at 45°C for 15 minutes.
  • An example low stringency wash for a duplex of, e.g., more than 100 nucleotides is 4-6X SSC at 40°C for 15 minutes.
  • stringent conditions typically involve sal concentrations of less than about L5 M, more preferably about 0.01 to 1.0 M, Na ion concentration (or other salts) at. pH 7.0 to 8,3, and the temperature is typically at least about 30°C and at least about 60 C C for long probes (e.g., >50 nucleotides).
  • Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide, in generaL a signal to noise ratio of 2X (or higher) than that observed for an mirelated probe in the particular hybridization assay indicates detection of a specific hybridization.
  • Very stringent conditions are selected to be equal to the T ra for a particular probe.
  • An example of stringent conditions for hybridization of complementar nucleic acids which have more than 100 complementary residues on a filter in a Southern or Northern blot is 50% formamide, e.g., hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37°C, and a wash in 0.1 X SSC at 60 to 65°C.
  • Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 .0 M NaCl, 1% SDS at 37°C, and a wash in 0.5X to IX SSC at 55 to 60°C.
  • “Operably-linked ' ' nucleic acids” refers to the association of nucleic acid sequences on single nucleic acid fragment so that the function of one is affected by the other, e.g., an arrangement of elements wherein the components so described are configured so as to perform their usual function.
  • a regulatory DNA sequence is said to be
  • Coding sequences can be operably-linked to regulatory sequences in sense or antisense orientation.
  • Control elements operably linked to a coding sequence are capable of effecting the expression of the coding sequence. The control elements need not be contiguous with the coding sequence, so long as they function to direct the expression thereof. Thus, for example, intervening untranslated yet transcribed sequences can be present between a promoter and the coding sequence and the promoter ca still be considered “operably linked" to the coding sequence.
  • isolated and/or purified refer to in vitro isolation of a. nucleic acid, e.g. , a DNA or RNA molecule from its natural cellular- environment, and from association with other components of the cell or test solution (e.g. RNA pool), such as nucleic acid or polypeptide, so that it can be sequenced, replicated, and/or expressed.
  • a. nucleic acid e.g. , a DNA or RNA molecule from its natural cellular- environment, and from association with other components of the cell or test solution (e.g. RNA pool), such as nucleic acid or polypeptide, so that it can be sequenced, replicated, and/or expressed.
  • isolated nucleic acid may be a DNA molecule containing less than 31 sequential nucleotides that is transcribed into an RNAi molecule.
  • RNAi molecule may, for example, for a hairpin structure with a duplex 21 base pairs in length that is complementary or hybridizes to a sequence in a gene of interest, and remains stably bound under stringent conditions (as defined by methods well known in the art, e.g., in Sambrook and Russell, 2001).
  • the RNA or DNA is "isolated” in that it is free from at least one contaminating nucleic acid with which it is normally associated in the natural source of the RNA or DNA and is preferably substantially free of any other mammalia RNA or DNA, The phrase "free from at least one.
  • nucleic acid molecules having base substitutions are prepared by a variety of methods known in the art. These methods include, but are not limited to, isolation from a natural source (in the case of natural ly occurring sequence variants) or preparation by oMgonucIeotide-mediated (or site-directed) mutagenesis, PCR mutagenesis, and cassette mutagenesis of an. earlier prepared variant or a non-variant version of the nucleic acid molecule.
  • “Operably- linked ' ' refers to the association of nucleic acid sequences on single nucleic acid fragment so that the function of one of the sequences is affected by another.
  • a regulatory DNA sequence is said to be “operably linked to” or “associated with” a DNA sequence that codes for an RNA or a polypeptide if the two sequences are situated such that the regulatory DNA sequence affects expression of the coding D A sequence (i.e., that the coding sequence or functional RNA is under the transcriptional control of the promoter). Coding sequences can be operably-linked to regulatory sequences in sense or antisense orientation,
  • nucleotide molecule As used herein, the term “derived” or “directed to” with respect to a nucleotide molecule means that the molecule has complementary sequence identity to a particular ⁇ molecule of interest,
  • Treating refers to ameliorating at least one symptom of, curing and/or preventing the development of a disease or a condition
  • protein protein
  • agents of the invention are preferably administered so as to result in a reduction in at least one symptom associated with a disease.
  • the amount administered will vary depending on various factors including, but. not limited to, the composition chosen, the particular disease, the weight, the physical condition, and the age of the mammal, and whether prevention or treatment is to be achieved. Such factors can be readily determined by the clinician employing animal models or other test systems, which are well known to the art.
  • Administration of the aptamer chimera may be accomplished through the
  • nucleic leic acid molecule administration of the nuc leic acid molecule.
  • Pharmaceutical formulations, dosages and routes of administration for nucleic acids are generally known in the art.
  • the present invention envisions treating a disease, for example, vascular stenosis, restenosis or stroke, in a mammal by the administration of an agent, e.g., a nucleic acid composition, an expression vector, or a viral particle of the invention.
  • Administration of the therapeutic agents in accordance with the present invention may be continuous or Intermittent, depending, for example, upon the recipient's physiological condition, whether the purpose of the administration is therapeutic or prophylactic, and other factors known to skilled practitioners.
  • the administration of the agents of the invention may be essentially continuous over a preselected period of time or may be in a series of spaced doses. Both local and systemic administration is contemplated.
  • One or more suitable unit dosage forms having the therapeutic agent(s) of the invention can be administered by a variety of routes including parenteral, including by intravenous and intramuscular routes, as well as by direct injection into the diseased tissue.
  • the formulations may, where appropriate, be conveniently presented in discrete unit dosage forms and may be prepared by any of the methods well known to pharmacy * Such methods may include the step of bringing into association the therapeutic agent with liquid carriers, solid matrices, semi-solid carriers, finely divided solid carriers or combinations thereof, and then, if necessary, introducing or shaping the product into the desired delivery system.
  • the therapeutic agents of the invention are prepared for administration, they are preferably combined with a pharmaceutically acceptable carrier, diluent or excipient to form a pharmaceutical formulation, or unit dosage form.
  • a pharmaceutically acceptable carrier diluent or excipient to form a pharmaceutical formulation, or unit dosage form.
  • the total acti ve ingredients in such formulations include from 0.1 to 99.9% by weight of the formulation.
  • a "pharmaceutically acceptable” is a carrier, diluent, excipient, and/or salt that is compatible with the other ingredients of the formulation, and not deleterious to the recipient thereof.
  • the active ingredient for administration may be present as a powder or as granules, as solution, a suspension or an emulsion.
  • compositions containing the therapeutic agents of the invention can be prepared by procedures known in the art using well known and readil available ingredients.
  • the therapeutic agents of the in venti on can also be formulated as solutions a ppropriate for parenteral administration, for instance by intramuscular, subcutaneous or intravenous routes.
  • the pharmaceutical formulations of the therapeutic agents of the invention can also take the form of an aqueous or anhydrous solution or dispersion, or alternatively the form of an emulsion or suspension.
  • the therapeutic agent may be formulated for parenteral administration (e.g., by injection, for example, bolus injection or continuous infusion) and may be presented in unit dose form in ampules, pre-filled syringes, small volume infusion containers or in multi-dose containers with an added preservative.
  • the active ingredients may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.
  • the active ingredients may be in powder form, obtained by aseptic isolation of sterile solid or by lyophilization from solution, for constitution with a suitable vehicle, e.g. , sterile, pyrogen- free water, before use.
  • the unit content of active ingredient or ingredients contained in an indi vidual aerosol dose of each dosage form need not in itself constitute an effective amount for treating the particular indication or disease since the necessary effective amount can be reached by administration of a plurality of dosage units. Moreover, the effective amount may be achieved using less than the dose in the dosage form, either individually, or in a series of administrations.
  • the pharmaceutical formulations of the present invention may include, as optional ingredients, pharmaceutically acceptable carriers, diluents, solubilizing or emulsifying agents, and. salts of the type that are well-known in the art.
  • pharmaceutically acceptable carriers such as phosphate buffered saline solutions pH. 7,0-8.0, saline solutions, and water.
  • MODS/ARDS multiple organ dysfunction syndrome
  • ARDS acute respiratory distress syndrome
  • ALI acute lung injur
  • Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response. Their potential to amplify tissue injury by killing other cells, in addition to their agonistic activity on T LRs, provides strong rationale to target histones for therapy. Since histones (cationic proteins) normally associate with DNA in the nucleosome, oligonucleotides (anionic molecules) have extraordinarily high affinity and specificity for histones, making them the preferred reagent for binding and neutralizing circulating histones. R A aptamers are designed to selectively target and neutralize circulating hisiones.
  • RNA aptamers are single-stranded oligonucleotides whose binding properties depend on their sequence and structure. Aptamers have significant advantages over other biologies, including stability at room temperature, resistance to serum degradation, and minimal iirrmunogenicity. By using chemically modified RNA, the aptamers are protected from serum degradation and immunogenicity.
  • MODS is described as ⁇ the development of potentially reversible physiologic derangement involving two or more organ systems not involved in the disorder that resulted in 1CU admission, and arising in th wake of a potentially life-threatening physiologic insult ' '.
  • ALT acute lung injur
  • ARDS acute respiratory distress syndrome
  • ALI/ARDS occurs as a consequence of critical illness of diverse etiologies including initial major trauma, toxic inhalation, burns, near-drowning, radiation, sepsis, and blood transfusion.
  • MODS haemostatic abnormalities ranging from subclinical activation of blood coagulation (hypercoagulability ⁇ to ae ' ute disseminated intravascular coagulation (DIC) and subsequent consumption of platelets and coagulation proteins causing bleeding.
  • Eukaryotie genomes are organized in chromatin;, a DNA-protein complex whose basic repeating unit is the nucleosome.
  • the nucleosome consists of DNA wrapped around an oc tamer of histone proteins (two each of hi stones H2A, H2B, 1 13. and H4).
  • NETs neutrophil extracellular traps
  • Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; 187: 160-169).
  • the present invention provides a therapeutic to rapidly neutralize circulating histones and terminate the self -propagating cycle of tissue injur ⁇ 1 and development of MODS/ARDS.
  • the ideal reagent. to bind histones is an oligonucleotide (anionic DNA or RNA molecule).
  • An innovative technology is used to identify short oligonucleotides (RNA ap tamers) that selectively bind to histones known to cause MODS/ARDS. Since histones are highly conserved across species from yeast to humans, the bio-reagents developed and validated are quickly advanced for testing in preclinical models and human clinical trials.
  • nucleosomes or DNA mediate these toxic effects.
  • nucleosorn.es which have been briefly sonicated or exposed to serum (i.e., DNA is degraded and individual hi stones are released) induce endothelial cell death.
  • Human patient serum with histone levels in excess of 50 ,ug/ml is toxic to cultured endothelial cells.
  • Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; 187: 160-169). Intravenous injection of recombinant histones in mice causes endothelial damage, cytokine elevation, platelet aggregation, and microvascular necrosis in. the lungs, leading to death.
  • (Abrams ST, Zhang N, Manson. J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K, Kipar A. Yu W. Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; ! 87: 1 0- 169; Xu J, Zhang X, Pe!ayo R, Monestier M, Ammo 11 o CT, Semeraro F, Taylor FB, Esmon NL. Lupu F, Esmon CT.
  • Extracellular histones mediate tissue damage through multiple mechanisms including: (1) disruption of cell membranes, resulting in Ca 2 ⁇ influx and subsequent elevations in intracellular Ca ⁇ + concentrations and cell damage; (2) activation of TLR2 and TLR4, thereby inducing NF- ⁇ and pro-inflammatory cytokine production; (3) induction of a pro-coagulant phenotype in platelets, thereby accelerating blood coagulation and enhancing thrombin generation in a mechanism that involves Toll like receptors (TLR) 2 and 4 (Semeraro F, Ammollo CT, Morrissey J ⁇ I, Dale GL, Friese P, Esmon NL, Esmon CT.
  • TLR Toll like receptors
  • toxic mediators i.e., cytokines
  • Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response; their potential to amplify tissue injuiy by kill ing other eel is, in addition to their agonistic activity on TLRs, provides a rationale to target histones for therapy.
  • approaches currently being pursued in experimental models have marked limitations.
  • TLRs clearly mediate the immune response induced by histories, they have no role in calcium-mediated cytotoxicity, negating the use of TLR2/4 neutralizing antibodies.
  • oligonucleotide anionic molecule
  • Histones normally associate with DNA in the nucleosome.
  • oligonucleotides e.g. RNA aptamers
  • RN A aptaniers are single-stranded nucleic acids whose binding properties depend on their sequence and structure.
  • RNA aptamers that selectively bind to human histones.
  • SELEX is used to identify RNA aptamers with high affinity to human 3-13 and 114. Studies characterize individual aptamer sequences and define their binding affinity and specificity. (Tuerk C, Gold L. Systematic evolution of ligands b exponential enrichment: RNA ligands to bacteriophage t4 DNA polymerase. Science. 1990;249:505-510). Studies also use cultured human pulmonary endothelial cells, alveolar epithelial cells, and platelets to confirm the efficacy of RNA aptamers at neutralizing histone-rnediated cellular effects.
  • RNA aptamers that selectively bind with high affinity to both human histones H3/H4.
  • RN A aptamers with specificity for human H3 and 114 were enriched by pre- clearing a chemically modified RNA aptamer library against control proteins (BSA and human IgG) abundant in serum. Those NAs that did not bind serum proteins were collected and incubated with either human H3 or H4. Histone-bound RNAs were collected and processed for the next round of selection. Several rounds of selection were performed to enrich for high affinity binders. High affinity aptamers were enriched by modifying the selection conditions at each round of selection (e.g.
  • RNA sequences were enriched with overall higher affinity (high pM range) for the intended histone target ( Figure 3B) (data shown for H3 selection). Finally, round 7 from each selection did not bind to proteins normally found in serum (dat not shown), confirming the specificity of the selected RNAs. After 8-10 rounds of selection, final post-selection, enriched aptamer pool has affinities in the low pM range.
  • the progress of the selections are assessed by determining the sequence diversity of the random regions from round to round by high- throughput sequencing (HTS) and identifying sequence and structure families using bioinformatics analyses. Progress of the selections is assessed by carrying out standard in vitro binding assays with the pools of RNA from each round.
  • HTS high- throughput sequencing
  • RNA aptamers identified from the sequence analysis are screened for (1 ) specificity of binding and for (2) ability to inhibit histone activity, fa vitro filter binding assays are performed with the top 10-15 sequences from the HI ' S and bioinformatics analysis as previously described.
  • Nucleotide bias observed with a short SELEX RNA aptamer library Nucleic Acid Ther, 201 i;21:253-2.63).
  • a more extensive quantitative determination of binding affinities via surface plasmon resonance (SPR) is performed for the 5 aptamers that demonstrate the greatest affinity and specific binding in the filter binding assays (Thiel KW, Hernandez LL Dassie JP, Thiel WH, Liu X, Stockdaie KR, Rothman AM, Hernandez FJ, McNamara JO, 2nd, Giangrande PH. Delivery of chemo-sensitizing siRNAs to her2+-breast cancer cells using R A aptamers. Nucleic Acids Res.
  • human pulmonary microvascular endothelial cells EC
  • human alveolar epithelial cells or human platelets are exposed to histories purified from calf thymus'" (contains a heterogeneous mixture of core histones)
  • Abrams ST Zhang N , Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K t Kipar A, Yu W. Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013:187: 1 0-169;
  • Extracellular histones promote thrombin generation through platelet-dependent mechanisms: Involvement of platelet t!r2 and tlr4. Blood. 201 1 ;1 18: 1952-1961 ) in the presence or absence of aptamer.
  • Human patient serum with histone levels in excess of 50 pg/ml is toxic to EC (Abrams ST, Zhang N, Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K, Kipar A, Yu W. Circulatin histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013;187: 160-169; Xu J, Zhang X, Pelayo R, Monestier M, Ammollo CT, Semeraro F, Taylor FB, Esmon NL, Lupu F, Esmon CT.
  • Platelet aggregation The release of histones from dying cells is associated with microvascular thrombosis. Histone H3 and 114 have been found to be responsible for directly inducing aggregation of human platelets. As an independent measure of aptamer inhibition of histone activity, the effect of individual, histone aptamers is evaluate on hi stone-mediate platelet aggregation. (Xu J, Zhang X, Pelayo , Monestier M, Ammollo CT. Scmeraro F, Taylor FB, Esmon NL, Lupu F, Esmon CT. Extracellular histones are major mediators of death in sepsis. Nature medicine. 2009; 15:1318-1321).
  • RNA aptamers did not reverse collagen-mediated platelet aggregation (Fig. 4B).
  • blood is collected from human volunteers, platelet-rich plasma prepared, and platelets isolated.
  • RNA aptamers Evaluate efficacy and safety of histone-speeifie RNA aptamers in vivo.
  • the efficacy of the histone speci fic aptamers in mouse models of MODS/ARDS, TRALI, and smoke inhalation is evaluated.
  • Safety is evaluated in immune -competent mice and human peripheral blood mononuclear cells (PBMCs).
  • efficacy in humans is evaluated by obtaining serum from critically ill patients and test the ability of aptamers to neutralize histone-mediated toxicity.
  • Animals are pretreated with the aptamers (0.1 mg kg or 1 mg/kg, IV) at various times related to the injury to test the ability lo prevent development or abort progression of tissue injury. The doses are adjusted as indicated based on the binding data to identity the lowest effective dose.
  • the aptamer is administered at various times after delivery of the histones (5, 15, and 30 minutes) to examine the ability of the aptamer to prevent injury after the presence of circulating histones.
  • Nonin vasive determination of respiratory function (reduced 0 2 saturation) is continuousl monitored using a pulse oximeter.
  • mice After 3h (or at the time of death if occurs prior to 3b) the mice are sacrificed and the following performed: ⁇ Alveolar permeability and inflammation: Tracheas are isolated and canriulated and the lungs flushed with sterile PBS. Cytospins of BALF cells are stained with modified Wright-Giemsa.
  • Alveolar permeability is assessed by quantitative detection of mouse albumin in BALF by ELISA.
  • H&E hematoxylin and eosin
  • TLR hematoxylin and eosin
  • Platelet FACS Platelet surface marker expression (activated u2b
  • N ' onspecific immune activation Thorough studies are performed to evaluate any potential nonspecific immune effect of the hi stone RNA aptamers. For these studies, doses of the RNAs that are 10-fold greater than those found to be effective in vivo, are injected in the tail veins of C57/BL6 male mice. Blood from treated animals is collected by cardiac puncture at I h, 6h and 12h after the first administration of the RNA. Collected blood sera is analyzed using the Bio-Rad Biople platform to assess the levels of 23 different cytokines ( aul R, Johnson , Scholz H. Man' G. Performance of the bioplex 2200 autoimmune vasculitis kit. Aiitoimmun Rev. 2009;8:224-227)..
  • Spleens and livers from treated mice will also are collected at early (O.Shrs), intermediate (6hrs), and late (24brs) time points following injection of the RNA and processed for total RNA.
  • Levels of inflammatory cytokines, interferons and viral RNA recognition genes are quantified using RT-qPCR as previously described.
  • Poly I:C Polyinosmicrpolycytidylic acid
  • Circulating histone levels are measured in the serum using an. ELISA assay.
  • the DNA aptamer librar contains a variable region of 20 base flanked by two constant regions ( Figure 5): 1) The DNA template library is in vitro transcribed into RNA using a modified T7 RNA polymerase enzyme that enables the incorporation of chemically modified NTPs. 2) The RNA library is incubated with an irrelevant protein (control protein) for the preelear step to remove non-specific binders. 3) Those RNA aptamers that do not bind the control protein are then incubated with a target protein.
  • RNA aptamers bound to target protein are recovered, reversed transcribed using RT-PCR, 5) PGR amplified, and 6) in vitro transcribed in the presence of chemically modified NTPs (e.g. 2 , -fluoro modified
  • Sequence enrichment of aptamers for istones (Figure 8). The sequence enrichment (% Enrichment) was determined for aptamers from the A) 113.2 and B) H4 aptamer selections. Sequence enrichment, was determined for each round of selection
  • Selection Round from read counts determined fro Illumina sequencing using the following equation; % Enrichment- 1 -(unique sequences/total sequences). Unique sequences refers to the number of different sequences identified within a selection round and total sequences refers to the total number of reads from a selection round including all duplicate reads.
  • the round representation analysis examines aptamer persistence by first determining the number of selection rounds each aptamer sequence was observed and second plotting the distribution of round representation for al l aptamers within each selection round. Aptamers with read counts significantly greater than the read counts observed in round 0 (>4 reads) and that appear in at least two selection rounds are classified as selected.
  • Histone aptamer sequences (Figure 10), These aptamers have read counts significantly greater than the read counts observed in round 0 (>4 reads) and that appear in at least two selection rounds are classified as selected. These nine aptamers had the largest Log2 fold change between rounds 6:8. They were further selected by their presence in either both or one of the selections. The full list of selected aptamers is in Table 1 below.
  • Nitrocellulose (top) and Nylon (bottom) double-filters using dot-blot apparatus (Na!gene, Rochester, NY). CL-X film was exposed by membranes for 30 minutes at RT and was imaged on the typhoon. ImageJ 1.38* software (Wayne Rasband; N3H, Bethesda, MD) was used to determine relative optical density of radiographed spots in NC-filter binding assay, these data been used to calculate amount of radioactive RNA in each spot. (Wong i, Lohman TM. A double-filter method for nitrocellulose-filter binding: application to protein-nucleic acid interactions. Proc Natl Acad Sci USA 1993; 90: 5428-32.)
  • Binding of individual RNA aptamers to histone H3 and H4 proteins ( Figures 16A-16I). Binding of aptamers KU1 (A), KU2 (B), KU 3 (C), KU4 (D), U5 (E), KU6 (F), U 7 (G), KU 8 (H), KU 9 (1) to histones 113 (light circles) and H4 (dark squares) measured by filter binding assay.
  • Binding affinities ( j ) of the different clones in Figures 16A-16I for H4 and 143 Figure 17. Binding of individual RNA aptamers to histone HI, H3, and H4 proteins ( Figures 18A-18I). Binding of aptamers KU 1 (A), KU2 (B), KU 3 (C), U4 (D). KU5 (E), KU6 (F), KU 7 (G), KU 8 (H), KU 9 (I) to histones Hi (triangles), H3 (light circles), and H4 (dark squares) measured by filler binding assay.
  • Binding of individual RNA aptamers to histone H3, H4, and Human Serum ( Figures 20.4-20! ). Binding of aptamers KU1 (A), KU2 (B), KU 3 (C), KU4 (D), KU5 (E), KU6 (F), KU 7 (G), KU 8 (H), KU 9 (1) to Human Serum (triangles), H3 (light circles), and H4 (dark squares) measured by filter binding assay.
  • Binding of individual RNA aptamers to histone J 13, 114, and Human Albumin ( Figures 22A ⁇ 22i). Binding of aptamers K I (A), KU2 (B), KU 3 (C), KU4 (D), KU5 (E), KU6 ( F). KU 7 (G), KU 8 (H), KU 9 (1) to Human Albumin (triangles), H3 (light circles), and H4 (dark squares) measured by filter binding assay,
  • Binding of individual RNA aptamers to histone HI , H2A, H2B, H3, and 114 proteins ( Figures 24A-24C). Binding of aptamer clones (A) KU5, (B) KU7. (C) KU9 to histones HI (circles), H2A (squares), H2B (upward triangle), H3 (downward triangle), and H4 (diamonds) measured by filter binding assay.
  • Binding affinities (3 ⁇ 4) of the different clones in figure 7 for histones II I , H2A, H2B, H3, and H4 ( Figure 25).
  • RNA aptamers inhibit histone-mediated platelet aggregation ( Figures 27A-27D).
  • Platelet aggregation was performed with washed human platelets and quantitated using an aggregometer at 2 min intervals.
  • Histones 113 and H4 induce platelet aggregation.
  • RNA aplanicrs have no effect on collagen-mediated platelet aggregation. Heparin (I IJ/mL) reverses hi stone- mediated platelet aggregation.
  • EA.hy926 cells were cultured with human ( A) histone 113, (B) histone H4, or (C) aptamers (KU , KU4, or NSC) for 1 hr at 37°C. Cell damage was measured by flow cytometry for propidium iodide staining.
  • Histone concentration 0, 12.5, 25, SO, 1.00 ⁇ . «/ ⁇ and aptamer concentration: 0.0, 12.5, 25, 50, 100 nM, NSC refers to a non-specific control aptamer sequence that does not bind histones.
  • RNA sequences were enriched with overall higher affinity (p ' M vs. nM range) for the intended histone target compared to the starling, unenriched RNA aptamer library (round 0, R0) ( Figure 12).
  • the progression of the H3.2 and 1-14 selections was assessed by determining the sequence diversity of the random regions from round to round by high -throughput sequencing (UTS) and identifying enriched sequence and structure families using bioinformatics analysis ( Figures 7-9). individual RNA aptamer candidates (9 total) identified from the
  • Binding data are shown for the top nine candidate aptamers (most highly enriched aptamers following 8 rounds of selection) identified from the bioinformatics analysis ( Figure 10). Although the aptamers were selected against H3.2 and 1 ⁇ 4, binding to histones i ll ( Figure 17- 18 and Figure 23-24), H2A and H2B ( Figures 23- 24) (the equilibrium dissociation constants are reported in the tables) were also determined. The binding data confirm that these aptamers bind with high affinity ( D - pM to low nM range) to histones H3.2 and H4 ( Figures 23-24).
  • RNAs do not bind to proteins in human serum ( Figures 9-20) or to human serum albumin (a protein which is highly abundant in serum) ( Figures 21-22).
  • NSC and A9g two control aptamers
  • Platelet aggregation ( Figures 25-26); The release of histones from dying cells is associated with microvascular thrombosis.
  • Histone H4 and, to a lesser extent H3.2, have been found to be responsible for directly inducing aggregation of human platelets by driving plasma thrombin generation through the activation of TLR2 and TLR4. It is shown that histones H3.2 and H4 induce pronounced platelet aggregation (plotted as % aggregation), which can be inhibited with addition of heparin (positive control) and the un selected RNA aptamer pool (R0).
  • RNA aptamers inhibition of hi stone-mediated platelet aggregation was more pronounced with selected histone aptamers (data shown for U7) compared to the unselected RNA pool (R0).
  • the RNA aptamers did not reverse collagen-mediated aggregation.
  • Aptamer safety profile in vitro Figures 27-31): Circulating hi stones cause endothelial eel! toxicity, contributing to vascular dysfunction, platelet aggregation and tissue ischemia. It was confirmed that the exposure of cultured epithelial cells with histone H4 (20 ng/ml for 1 hours) results in cell death. In contrast, the candidate histone-selectiye aptaniers ( U5, U7, KU9) did not induce cell toxicity (1 ⁇ for 1 hour),
  • Histone administration causes neutrophil migration, endothelial injury and
  • HTS read variable region R A sequences ft in H3 selection selection Selected sequenc TTTGAAGGGTGATGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGUCCGCAGACGACUCGCCCGA 0056.
  • HTS read variable region R A sequences # in H3 selection selection Selected sequenc
  • GCCTTTCATTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCAUUCUGCCCUCCCCAGACGACUCGCCCGA 0316. .H32 0274. .H04
  • HIS read variable region A sequences U in H3 selection selection Selected sequenc

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Abstract

The present invention relates to optimized aptamers and methods of using these aptamers.

Description

NUCLEIC ACID APTAMERS TO TREAT HISTONE-INDUCED DISEASE STATES
Figure imgf000002_0001
This application claims the benefit of priority of U.S. Provisional Application Serial No. 62/095,530 filed on December 22, 2014, which application is herein incorporated b reference.
BACKGROUN D OF THE INVENTION
A challenging medical problem often observed in critically ill patients is that in response to severe injury or illness, even those organs not. directly affected by the original problem become dysfunctional. For example, patients with severe infection will often develop respiratory failure requiring mechanical ventilation, renal failure requiring dialysis, hepatic dysfunction, coagulation abnormalities, and hypotension requiring vasopressors. This condition, known as the multiple organ dysfunction syndrome (MODS), or by some of its more prominent mani festations such as acute respiratory distress syndrome (ARDS), can be reversible, but the only treatment is supportive care as there is no therapy to directly prevent or reverse MODS. The incidence of MODS in intensive care unit (ICU) patients is 10-40% and the incidence of death in MODS is 40-50%. Even with survival, organ recovery can take months with significant associated morbidity and cost.
The annual, incidence of acute lung injury (AO") and acute respiratory distress syndrome (ARDS) approximates 200,000 cases in the United States, with mortality approaching 40%. Despite extensive research into the pathogenesis of ALI and clinical trials testing new therapeutics, the improvement in outcomes following ARDS over the past decade are due to improved strategies of mechanical ventilation and advanced support of other failing organs, as there remains no effective pharmacotherapy to treat patients with this syndrome. Furthermore, patients who survive frequently have significant psychological and physical morbidity, residual physical limitations and poor quality of life. The development of AO/ ARDS occurs as a consequence of critical illness of diverse etiologies: however, many of these are highly relevant to the military. For example, despite surviving an initial major trauma (e.g., blast and/or explosive), there is a high mortality associated with the subsequent development of multiple organ dysfunction syndrome (MODS). Postmortem findings show the lungs to be more frequently affected than any other organ in patients who die after trauma. In addition to trauma, other combat and military related causes of ALI/ ARDS include toxic inhalation, burns, near-drowning, radiation, sepsis, and blood transfusion. The underlying pathological changes include neutrophil infiltration, pulmonary edema, hemorrhage, and microvascular thrombosis. There are no current methods to prevent the onset of MODS, only treatmen of its effects. Thus, there is an on-going need for effective treatments to prevent the development of these syndromes.
SUM ARY OF THE INVENTION
The present invention is transformative because it develops an innovative treatment with preventing the development of MODS/ARBS in high risk patients. Extracellular histones are targeted with RNA aptamers in order to terminate the self-propagating cycle of tissue injury responsible for MO S/ARDS. By interrupting the cycle of histone-mediated cell injury, these histone-specific aptamers save lives and reduce the morbidity and the financial cost of multiple severe illnesses in thousands of patients.
Certain embodiments of the present invention provide a nucleic acid molecule not more than 100 nucleotides in length (e.g., 50, 51 , 52, 53, 54, 55, 56, 57, 58, 59, 60. 61 , 62, 63, 64, 65, 66, 67, 68, 69, 70, 71 , 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91 , 92, 93, 94, 95.96, 97, 98, 99, or 100 nt) comprising an aptamer, wherein the aptamer specifically targets histones. In certain embodiments, the aptamer specifically targets both H3 and/or H4. In certain embodiments, the aptamer specifically targets both human H3 and 114.
In certain embodiments, the nucleotides are RNA. In certain embodiments, the nucleic acid of the present invention is DNA. In certain embodiments, the RNA inciudes a modified nucleotide.
In certain embodiments, the nucleic acid molecule comprises a chemically modified RNA comprising a 2' substituted sugar such as 2'-0-methyl-; 2'-0-(2-methoxyeth l); 2-0- alkyl; 2-O-allyl; 2'-S-alkyi; 2'-S-alIyl; 2 -fluoro-; 2'-amtno; 2'-halo or 2-azido-ribose, a carbocyclic sugar analogue, an a-anomeric sugar; an epimeric sugar such as arabtnose, xylose or lyxose, a pyranose sugar, a furanose sugar, sedoheptulose 2'-£luoro~p~D-arabinonueleotide (FANA) modification: a Locked nucleic acid (LNA).
In certain embodiments, the 2'OMe modified bases are alternated with un-modified RNA bases.
In certain embodiments, the chemically modified RNA comprises a 2'-fluoro
pyrimidine and/or a 2* O-methyl pyrimidine.
In certain embodiments, the nucleic acid molecule comprises an 2'OMe purine and a 2'-fluoro pyrimidine. in certain embodiments, 'the nucleic acid comprises bridging phosphorothioate, and end cap that reverses the polarity of the chain and/or a linker.
Certain embodiments of the present invention provide a conjugate comprising the nucleic acid molecule described above linked to a therapeutic or diagnostic molecule.
Certain embodiments of the present invention provide a pharmaceutical composition comprising a molecule or conjugate as described above and a pharmaceutically acceptable carrier.
Certain embodiments of the present invention provide a method for delivering a therapeutic or diagnostic molecule to a subject having or being disposed to having
MQDS/ARDS, comprising contacting the subject with the conjugate described above, in certain embodiments, the administration is by intravenous injection or by inhalation. In certain embodiments, the composition is administered within 0-24 hours after an injury. In certain embodiments, the composition is administered within 0-12 hours after an injury, in certain embodiments, the injury is trauma, burn, sepsis, transfusion -related acute lung injury, organ ischemia or diagnosis of illness, or inhalation lung injury, in certain embodiments, the composition is administered in response to a prolonged severe illness. In certain
embodiments, the composition is administered multiple times, such as two, three, four, five or six times.
Certain embodiments of the present invention provide a use of a molecule or conjugate as described above for treating ODS/ARDS.
Certain embodiments of the present invention provide a molecule or conjugate as described above for use in therapy.
Certain embodiments of the present invention provide a molecule or conjugate as described above for use in the prophylactic or therapeutic treatment of a hi stone-i nduced disease. In certain embodiments, the disease is autoimmune disease (such as systemic lupus erythematosus), arthritis, rheumatoid arthritis, juvenile arthritis; edema; sepsis; septic shock; inflammation; non-septic hyper inflammatory disorder; infectious disease; thrombosis;
nephritis; inflammatory liver injury; traumatic hemorrhage; acute pancreatitis; acute respiratory distress syndrome; ischemic injury; ischemia-reperfusion injury; ischemic stroke; cardiovascular disease; atlierosclerosis: myocardial infarction; radiotherapy toxicity; cytokine therapy toxicity; granulomatous disease; asthma; gra t-vs. host disease, cachexia, a coagulopathy; inhalation inj ry, trauma, cancer; or burn effects, multiple organ dysfunction syndrome (MODS)/acute respiratory di tress syndrome (ARDS), and complications thereof in certain embodiments, the method further comprising administering at least one other therapeutic agent to the subject, before, concurrently with or after the composition. The present inventio further provides a nucleic acid coding molecule encoding a nucleic acid aptamer molecule as described above.
In certain embodiments, the therapeutic agent is selected from the group consisting of an antibody, an antibody fragment, an immune conjugate, a radionuclide, an
immunomodulatory, an anti-angiogenic agent, a pro-apoptotic agent, a cytokine, a chemokine, a drug, a toxin, a hormone, an siRNA, a cytokine, a chemokine, a coagulation inhibitor, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony sttmuiating factor, an interferon, erythropoietin, thrombopoietm, an enzyme, recombinant human thrombomodulin and activated human protein C.
As used herein the term ''therapeutic effect' ' refers to a change in the associated abnormalities of the disease state, including pathological and behavioral deficits; a change in the time to progression of the disease state; a reduction, lessening, or alteration of a symptom of the disease; or an improvement in the quality of life of the person afflicted with the disease. Therapeutic effect can be measured quantitativel by a physician or qualitatively by a patient afflicted with the disease state targeted by the aptamer. In certain embodiments wherein both the mutan and wild type allele are substantially silenced, the term therapeutic effect defines a condition in which silencing of the wild type allele's expression does not have a deleterious or harmful effect on nonnal functions such that the patient would not have a therapeutic effect.
in one embodiment, the RNA aptamers are constructed using known techniques to at least provide as operatively linked components in the direction, of transcription, control elements including a transcriptional initiation region, the DNA of interest and a
transcriptional termination region. The control elements are selected to be functional in a mammalian cell. The resulting construct which contains the operatively linked components is flanked (5' and 3') with functional sequences, such as sequences encoding an aptamer.
In one embodiment, pharmaceutical compositions will comprise sufficient genetic material to produce a therapeutically effective amount of the aptamer of interest, i.e., an amount sufficient to reduce or ameliorate symptoms of the disease state in question or an amount sufficient to confer the desired benefit. The pharmaceutical compositions will also contain a pharmaceutically acceptable excipient. Such excipients include any pharmaceutical agent that does not itself induce the production of antibodies harmful to the indi vidual receiving the composition, and which may be administered without undue toxicity. Pharmaceutically acceptable excipients include, but are not limited to, sorbitol, TweenSO, and liquids such as water, saline, glycerol and ethanol. Pharmaceutically acceptable salts can be included therein, for example, mineral acid salts such as hydrochlorides, hydro bromides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, malonates, benzoates, and the like. Additionally, auxiliary substances, such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles. A thorough discussion of pharmaceutically acceptable excipients is available in
REMINGTON'S PHARMACEUTICAL SCIENCES (Mack Pub. Co., N.J. 1991 ). BRJEF DESCRIPTION OF DRAWINGS
Figure 1 . Pathways of critical illness.
Figures 2A-2B. Extracellular histories mediate self-propagating diverse tissue injur}'. Figures 3Λ-3Β. Binding of RNA aptamers to histone H3 and H4 proteins. (A) Binding affinities of H3/H4 to unselected RNA aptamer library as determined by a dot blot filter binding assay. H3 j - 2.1 nM; H4 KD " 1 -5 nM. (B) Binding affinities of histone ID to unselected RNA aptamer library (RO) and selected RNA aptamer pools from rounds 2, 4, 6, and 7 of selection were determined as in part A. A leftward shift in binding is indicati ve of enrichment for higher affinity aptamers. D for rounds 6 (yellow) and 7 (blue) = 0.3 nM.
Figures 4.4 and 4B. RNA aptamers inliibit hi stone-mediated platelet aggregation. Platelet aggregation was performed with washed human platelets and quantitated using an aggregometer at 2 mln intervals. (A) Histories H3 (50 mg mt -- top row) and H4 (10 mg/mL - bottom row) induce platelet aggregation. Addition of the unselected round 0 (R0) aptamer pool (10 nM) reduces platelet aggregation. A more pronounced inhibition of platelet aggregation Is observed with selected RNA pools (R5 for H3 and R3 for H4). (B) RNA aptamers have no effect on collagen-mediated platelet aggregation (top panels). As a positive control, heparin (HJ/mL) reverses histone-mediated platelet aggregation (data shown for histone 114).
Figure 5. Schematic of in vitro SELEX technology.
Figure 6. Aptamer Inhibitors of Extracellular Histories for the Treatment of Critical Illness.
Figure 7. Complexity assay.
Figures 8A-8B. Sequence Enrichment data.
Figures 9A-9B. Bioinformatics analysis of selected rounds.
Figure 10. Selected aptamer sequences #1 . Figure 11. Predicted secondary structure of selected aptamer sequences. Figure 12. Double-filter binding assay.
Figures I3A-I3B. Binding data of rounds 0 and 8 of selection.
Figures 14A-14B, Binding data of rounds 0 and 8 of selection to calf thymus hi stones.
Figures 15A-15B. Binding specificity of selected round 8 RNA pool.
Figures 16A-16I. Binding of individual RNA aptamers to hi stones H and 114.
Figure 7. 1 able of binding affinities.
Figures J8A-18I. Binding data of individual RNA aptamers to histones H I , 113 and H4.
Figure 19. Table of binding affinities.
Figures 20A-2 I. Binding data of individual RNA aptamers to histories I B and H4, and human serum.
Figure 21. Table of binding affinities.
Figures 22A-221. Binding data of individual RNA aptamers to histones 113 and 114, and human albumin.
Figure 23. Table of binding affinities.
Figures 24A-24C. Binding data of individual RNA aptamers to histones Hi , H2A, H2B, H3 and 114 proteins.
Figure 25. Table of binding affinities.
Figure 26. Schematic of platelet aggregometer.
Figures 27A-27D. Platelet aggregation data.
Figure 28. Cytotoxicity data.
Figure 29. Cytotoxicity data.
Figure 30. Cytotoxicity data.
Figure 31. Cytotoxicity data.
Figure 32. Mouse data.
DETAILED DESCRIPTION OF THE INVENTION
Multiple organ dysfunction syndrome (MODS) is most commonly associated with trauma, sepsis or shock; however a common risk factor appears io be tissue injury (Figure I ). In addition to trauma and sepsis, the development of MODS/'ARDS is associated with a diverse group of clinical scenarios, including pancreatitis, cancer, peritonitis, surgery, acute lung injury, isehemia-reperfusion. radiation, burns., transfusion reactions, and auto- inflammatory disorders. The organs most affected histologically in MODS are in descending order: the lungs, liver, kidney, heart, gut, brain, pancreas and adrenals, though it i likely that most if not all organs are ultimately affected. In addition, disseminated intravascular coagulation is an insidious manifestation of MODS. Endothelial cell death and dysfunction underlies much of the systemic process, resulting in microcireulatory dysfunction and consequent capillary leak, interstitial edema, hemorrhage, and cellular infiltration.
Circulating bistones mediate detrimental effects in patients. Recent evidence has suggested that there may be a shared molecular mechanism responsible for the acute lung injury associated with these diverse disorders - and that involves extracellular hi stones. Eukaryotic genomes are organized in chromatin, a DNA.~prot.ein complex whose basic repeating unit is the nucleosome. The nuc!eosome consists of DNA wrapped around an octamer of histone proteins (two each ofhistones HS2A, H2B, H3, and H4). However, severely injured tissues release large quantities of nucleosomes into the circulation, which are further broken down into individual histones. Elevated levels of circulating histories can be derived eithe from damaged and apoptotic cells or from the degradation of neutrophil extracellular traps (NETs), which are structures of extracellular histones and DNA that ensnare and kill bacteria. In severely ill patients, an increase in histone levels within 6 hours of admission predicts for mortality. Similarly, in patients with majo trauma, circulating histones levels correlate with injury severity, elevated by 4 hours and peaking at about 24 hours after trauma and still detectable at 72 hours. In addition, extracellular histones have been detected in bronco alveolar lavage fluids (BALF) from patients with ARDS but not. in BALF from non-A DS patients. Transfusion-related acute lung injury (TRALi) is the leading cause of death from transfusion therapy in the US. N ETs and circulating histone levels are substantially higher in mice and humans with fatal TRALI.
Several lines of evidence have substantiated that circulating histones, not
nucleosomes or DNA, mediates these toxic effects. For example, nucleosomes which have been briefly sonicated or exposed to serum (i.e., DNA is degraded and individual histones are released) induce endothelial cell death, i lumaii patient serum with histone levels in excess of 50 g ml is toxic to cultured endothelial cells, intravenous injection of recombinant histones in mice causes endothelial damage, cytokine elevation, platelet aggregation, and
microvascular necrosis in the lungs, leading to death.
Extracellular histones mediate tissue damage through multiple mechanisms (Figure 2) (Chen, R., et a!., Release and activity of histone in diseases. Cell Death Dis, 2014. 5: p. el 370). First, histones disrupt ceil membranes, resulting in Ca"+ influx and subsequent elevations in intracellular Ca2" concentrations and cell damage. In this way, the tissue injury is amplified through release of additional histones into the circulation. Second, histones bind to and activate TLR2 and TL 4, thereby inducing NF-κΒ and pro-inflammatory cytokine production. Third, histones have been shown to induce pro-coagulant phenotype in platelets, thereby accelerating blood coagulation and enhancing thrombin generation in a mechanism that involves Toll like receptors (TLR) 2 and 4. Recently, neutrophil
extracellular traps were shown to activate platelets leading to thrombosis, and the major contributor to this process was hisione 114.8 A fourth mechanism is through increased gut permeability, which allows endotoxin or bacteria to enter the circulation and prime neutrophils. Activated neutrophils then release toxic mediators (i.e., cytokines) to damage lungs and other organs. Consistent with this idea, it has been reported that cytokines, including tumor necrosis factor (TNF)-a, interleukin (1L)~6 and IL-10, are elevated in mice 2 hours after exogenous hi stone infusion.
Current attempts to abrogate histone -induced injury. Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response; their potential to amplify tissue injury by killing other cells in addition to their agonistic activity on TLRs provides a rationale to target histones for therapy. Unfortunately, approaches currently being pursued in experimental models have marked limitations. First, while TLRs clearly mediate the immune response induced by histones, they have no role in the calcium-medi ted cytotoxicity, negating the use of TLR2/4 neutralizing antibodies.
Second, several other biologies that demonstrated efficacy in animal models have failed to provide a therapeutic benefit in clinical trials (activated protein C) or have increased risk of bleeding (heparin, APC) or toxicity (hi stone deacetylase inhibitors). In addition, many biologies require special handling and storage, special dosing considerations, and risk allergic reactions (recombinant proteins and antibodies), which limit their use in situations that are unique to military operations. The development of selective inhibitors of histone-mediated injury is a unique clinical opportunity to interrupt a pathophysiologic cascade responsible for significant morbidity and mortality.
R A aptamers are single-stranded nucleic acids whose binding properties depend on their sequence and structure, Aptamers have high binding affinity and specificity with significant advantages over other biologies, including stability at room temperature, resistance to serum degradation, and minimal immunogenicity. Proposed studies will use the in vitro selection technique SELEX (Systemic Evolution of Ligands by Exponential enrichment) to isolate high affinity RNA aptamers against human histones and test efficacy in human pulmonary microvascular endothelial cells and in animal models of histone-mediated ALL
While aptamers are analogous to antibodies in their range of target recognition and variety of applications, they possess several ke advantages over their protein counterparts: (I ) they are self-refolding, single-chain, and redox-insensitfve, and unlike proteins do not aggregate. They tolerate pH and temperatures that proteins do not. (2) They are easier and more economical to produce. Their production does not depend on bacteria, ceil cultures or animals. (5) They can easily be chemically modified to yield improved properties and nuclease resistance for plasma stability (6) Their small size leads to a high number of moles of target bound per gram, and transport properties allowing improved tissue penetration (7) They are much more stable at ambient temperature than antibodies yielding a much higher shelf life, and they can tolerate transportation without any special requirements for cooling, eliminating the need for a continuous cold chain. Another important advantage of aptamers is the feasibility of generating cross-species aptamers that enable testing the same reagent in preclinical animal models and in future human clinical trials. Finally, the clinical potential of aptamers is highlighted by the FDA approval of an aptamer-based drug for macular degeneration and by clinical trials that demonstrate the safety and efficacy of systemicaily administered RNA.
MODS is described as "the development of potentially reversible physiologic derangement involving two or more organ systems not involved in the disorder that resulted in ICU admission, and arising in the wake of a potentially life-threatening physiologic insult". With the lungs being the organ most commonly involved, acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) may be the primary and most clinically relevant presentation. The annual incidence of ALI/ARDS approximates 200,000 cases in the United States, with mortality approaching 40%. Despite extensive research into the pathogenesis of ALI and clinical trials testing new therapeutics, the improvement in outcomes following ARDS over the past decade are due to improved strategies of mechanical ventilation and advanced support of other failing organs, as there remains no effective pharmacotherapy to treat patients with this syndrome. Furthermore, patients who survive frequently have significant psychological and physical morbidity, residual physical limitations and poor quality of life. The development of ALI/ARDS occurs as a consequence of critical illness of diverse etiologies including initial major trauma, toxic inhalation, burns, near-drowning, radiation, sepsis, and blood transfusion. Furthermore, another common manifestation of MODS is haemostatic abnormalities ranging from subclinical activation of blood coagulat ion (hypercoagulability) to acute DiC and subsequent consumption of platelets and coagulation proteins causing leeding.
in response to apoptotic signals, core histones separate from genomic DMA, which results in hisione cytoplasmic translocation and subsequent release into the extracellular space. By causing tissue injury (Figure 2) histones are also involved in a self-sustaining cascade of apoptosis and subsequent histone release, in vivo, histone administration also accelerates cytokine release, endothelial damage, coagulation activation, and lung injury in animal models. Extracellular histones have been implicated in the development of many different disease conditions:
Acute lung injury. Extracellular histones have bee detected in Broncho alveolar lavage fluids (BALE) from patients with A DS but not in BALE from non-ARDS patients.
Transfusion related acute lung injury. Transfusion-related acute lung injury (TRALI) is the leading cause of death from transfusion therapy in the US. Neutrophil extracellular traps and circulating histone levels are substantially higher i mice and humans with fatal TRALI.
Sepsis. Mice show increased levels of histories in serum after endotoxin
administration, I B and H4 are the major components responsible for this toxicity. Endothelial disruption and consequential coagulation disorders are essential in pathogenesis of sepsis, with 35%of patients with sepsis developing DIC. Changes in the integrity of endothelium are the basis of these severe and mostly fatal complications of sepsis.
Trauma and burns. Histones are released following trauma or severe cellular stress. A cohort study of 52 patients shows that serum histone levels are significantly elevated after severe non-thoracic blunt trauma. Increased serum histones were positively and negatively related to injury severity score and Glasgow Coma Score, respectively High serum histone levels positively correlate with severe complications and poor prognosis.
Ischemia/reperfiisian and drug-mediated tissue injury result in sterile inflammation. Serum histone levels are significantly elevated in animal models with liver, kidney, and brain injury , suggesting an important role of histones in the regulation of sterile inflammation . Indeed, circulating histones are major mediators of animal death in several liver injury models Including coneanavalin A-triggered liver injury, aeetaminophen-induced.
hepatotoxicity, liver I/R, and acute liver failure. Extracellular histones function as damage- associated molecular patterns (DAMPs) and mediate sterile inflammation and organ damage.
Coagulatio and thrombosis. Histone administration in mice increases platelet aggregation and subsequent platelet-dependent thrombin formation, and microvascular thrombosis. Of them, H4 has the strongest impact on platelet activity. Histones also induce a pro-coagulant phenot pe in human platelets, which enhance thrombin generation mid accelerate the blood clotting process. Exogenous histones dose dependently increase plasma thrombin generation in the presence of thrombomodulin.
Autoimmune and aiU -inflammatory disorders. Hi stone release from NETosis has been implicated in a number of autoimmune and auto- inflammatory diseases such as rheumatoid arthritis, systemic lupus, small-vessel vasculitis.
Cerebral infarct. High concentrations of serum nucleosomes are detected in patients with cerebral stroke, especially in patients with large infarction volumes. Serum nucleosome levels rise quickly after post-ischemia, peak at days 3-5, and then fall slowly.
Cancer. Higher concentrations of circulating nucleosomes occur in tumor entities that are highly active or that are detected at advanced stages. An increase in baseline values of circulating histones indicated disease progression, whereas a decrease of the baseline values was an indicator of disease regression.
Ebola and related viral infections'. Ebola virus is very pathogenic in humans and induces an acute hemorrhagic fever that leads to death in about 70% of patients. Viral hemorrhagic fever (VHF) is a severe febrile illnesses caused by enveloped RNA viruses from 4 taxonomie families: Arenaviridae, Bunyaviridae, Filoviridae, and Flaviviridae . As the disease progresses, vascular damage with capillary leakage ma cause nondependent edema and serous effusions of body cavities such as the pleural and peritoneal spaces. Hemorrhages occur when the patient has thrombocytopenia or severe platelet dysfunction. The extensive multifocal necroses in filoviral infections are probably caused, at least in part, by ischemia associated with fibrin thrombi caused by disseminated intravascular coagulation. The lysis of Ebola-infected cells such as monocytes and macrophages may partially account for cell death. The massive fragmentation of DNA found in the blood and apoptosis was observed early during disease in all fatalities studied, whereas hemorrhagic signs were observed only in some terminal patients.
Development of RNA aptamers to human histories H3 and H4, The goal of these studies is to identify RNA aptamers that selectively bind with high affinity to both human histones H3/H4. An RNA librar of 10 RNA sequences (with 2'-iluoro modified
pyrimidines) consisting of 51 nucleoli de-long RNA oligonucleotides that include a random region of 20 nucleotides and two constant flanking regions of known sequence (these enable PGR amplification at each selection round). For the first round of selection, the RNA library is pre-cleared against control proteins BSA and human IgG to remove nonspecific binders and removed by capturing the RNA-protein complexes onto a Centrex nitrocellulose centrifugal filter (high protein/low nucleic acid binding filter). Those RNAs that do not bind BSA or human IgG (flow-through after filtering) are collected and incubated with
commercially available recombinant huma histone H3. The RNA aptamers that bind to historic 113 are captured onto a second Centrex nitrocellulose centrifugal filter, eluted off the filter with chloroform and extracted. The RNA aptamers are then amplified for the next round of selection with RT-PCR using primers specific to the flanking constan regions of the RNA library followed by in vitro transcription via a T7 promoter included in the S'-PCR primer. For round two of the selection, the pool of RNA derived from round one is pre-Incubated with BSA and human IgG for the counter-selection step and the flow-through, containing RNAs that do not bind BSA or human IgG, incubated with commercially available recombinant human histone f 14. 'Those RNAs that bind histone H4 are eluted and amplified as described above. Selection alternates between human histone H3 and H4 for several rounds in order to isolate aptamers that bind to both of these proteins. High affinity aptamers are enriched by modifying the selection conditions (e.g. increasing the RNA: hi stone ratio during the positive selection step and reducing the incubation time of aptamers with histones H3/H4). The progress of the selection is assessed by determining the sequence diversity of the random regions from round to round by high-throughput sequencing (HTS) and identifying sequence and structure families using bioinformatics analyses. Progress of the selection is assessed by carrying out standard in vitro binding assays (e.g. double-filte nitrocellulose filter binding assays or surface plasmon resonance measurements) with the pools of RNA from each round.
Characterization of individual aptamer sequences. Individual RN A aptamers identified from the sequence analysis are screened for ( 1) specificity of binding and for (2) ability to inhibit histone activity. In vitro filter binding assays are performed with the top 10- 15 sequences from the HTS and bioinformatics analysis. Radiolabeled aptamers re incubated with varying amounts of control proteins (BSA and human IgG) or histone H3 or H4 proteins for 5 nitn prior to loading onto a dot-blot apparatus. Aptamers bound to histone proteins are retained onto the nitrocellulose filter while unbound aptamers will be retained onto the nylon filter. Membrane filters are quantified using a phospho imager screen and a corrected fraction bound will be determined for each aptamer. Binding specificity is assessed by determining background binding of each aptamer to control proteins (BSA and IgG). A more extensive quantitative determination of binding affinities via surface plasmon resonance is reserved for the 5 aptamers that demonstrate the greatest affinity and specific binding in the filter binding assays. For this work, biotin is coupled to the aptamers during chemical synthesis then immobilized on streptavidin-coupled chips using standard protocols. Recombinant human h stone H3 and H4 proteins are injected over the sensor surface for 5 min (association and dissociation time). Various concentrations of histone proteins are injected by serially diluting samples. Concentrations are adjusted based on the binding signal response to obtain optimal affinity determinations. The selectivity studies are carried out by injecting comparable concentrations of several control proteins (i.e. BSA and human IgG) over the immobilized aptamers. The dissociation constant. (Kn) for each aptamer is calculated by global fitting of four concentrations of histone proteins, assuming a constant density of aptamers on the surface of the chip. A 1 :1 binding mode with mass transfer fitting is used to obtain the kinetic data. BSA and human IgG measurements are aligned to histone I B and 114 data for the nonspecific analysis.
Assess functional efficacy of histone specific UNA aptamers. These studies test the abilit of the RNA aptamers to neutralize the toxic effects of histones in vitro and in vivo. The best two candidate aptainers that selectively bind H3/H4 in the low pM-low nM range as determined above are used in these studies. Mutated aptamers that do not bind histones serve as negative control aptamers. These studies utilize chemically synthesized aptamers to maximize reproducibility, serum stability, and prevent immune activation. To model circulating histone content, human pulmonary microvascular endothelial, cells (EC) or C57B1/6 m ice re exposed to histones purified from calf thymus (contains a heterogenous mixture of core histones ) in the presence or absence of aptamer. Human patient serum with histone levels in excess of 50 ,ug/ml is toxic to EC2; therefore this concentration is used in vitro. Dose response curves for the aptamers are determined in vitro and define the concentration used in in vivo studies.
Endothelial cell culture: Endothelial monolayers are treated with histones (SO pg/ml) with and without one of three aptamers (negative binding, 2 histone binding) at varying aptamer concentrations, based on the KD for each aptamer determined above (typical range is liim-lOOnm). Cells also receive aptamers without histones as a control. The following measurements are made: Measurement of calcium influx. Intracellular calcium concentration is measured using established protocols with fura 2-AM as the fluorescent probe.
Measurement ofTlR activation. Expression of IF -γ, IL-Ι β, 1L-6, and TNF-a by RT-
PCR.
Detection of cell toxicity. Live, apoptotic, and dead cells are determined by flow cytometry following staining with annexin-V and propidium iodide. Platelet activation: Blood Is collected from human volunteers, platelet-rich plasma prepared, and platelets isolated. Using the histone and aptaraer treatment protocol described above, studies are performed in a collagen- coated flow chamber perfused with washed human platelets. The surface area covered by platelet aggregates is quantified. Shear stress conditions are adjusted to replicate the values for arterial (-20 dynes/cm") or venous shear stress (~3 dynes/cm'). In separate studies, platelet surface marker expression (activated a2b 3 and P-se!ectin) are detected by fluorescent antibodies followed by flow cytometry.
Animal model. The best evidence of whether A LI has occurred in an animal is provided by a measurement of the cellular response (such as neutrophil counts in the BALF), a measurement of the integrity of the alveolar capillary barrier (such as movement of high molecular weight proteins from the serum into the airspaces) and histological images showing lung injury. Histones are injected into the tail vein of C57BL/6 mice (50 nig/kg body weight). The two aptamers identified in the in vitro experiments- tha are most effective and have greatest binding are tested. Animals are- pretreated with a control aptamer or one of the two histone aptamers (1 mg/kg or 5 mg kg, IV) 10 minutes prior io histone administration. These two doses of aptamers represent 'low" and "high" doses and are adjusted as indicated on the binding data obtained above. In separate experiments, aptamers are administered at various times after delivery of the histones (5, 15, and 30 minutes) to examine the ability of the aptamer to prevent injury after histone release. Noninvasive determination of respiratory function (reduced 02 saturation) is continuously monitored using a pulse oximeter. After 3 hours (or at the time of death if occurs prior to 3 hours) the mice are killed and the following performed:
TLR activation Blood is collected and serum isolated and stored at -80°C.
Cytokines/chemokines are all quantified using a customized multiplex magnetic bead panel kit.
Alveolar permeability and inflammation: Tracheas are isolated and cannulated and the lungs flushed with sterile PBS. Cytospins of BALF cells are stained with modified Wright- Gierasa. Alveolar permeability is assessed by quantitative detection of mouse albumin in BALF by ELISA.
Lung histology. Lungs are fixed in 4% (w/v) paraformaldehyde for 24 h and embedded and sectioned. Sections are stained with hematoxylin and eosin (H&E) and Mallory's phosphotungstic acid-hematoxylin staining for fibrin then examined by a pathologist for evidence of neutrophils in alveolar spaces, capillary congestion, intovalveolar hemorrhage, fibrin deposits and thrombi. Statistical analysis. All data is expressed as mean ± SEM. Statistical analysis is carried out using computer software SYSTAT. Results are analyzed using paired and unpaired t-tests for comparisons between two groups. For multiple, comparisons, mixed- model of ANOVA is employed to compare different treatment groups. Differences between mean values of multiple groups are analyzed with a Newman-Keuls post-hoc test.
Differences are considered statistically significant at values of <0.05.
Animals: The ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines have been reviewed and are followed for reporting of research findings. Studies are performed in male and female C57BL/6 mice (-10 wks of age), using the minimum number of mice required to address the descri bed protocols. For the animal studies, a power analysis assumin a 25% difference between the control and treatment groups with a standard deviation of 20% for both groups, an alpha error level of 5% (two sided) and a beta error level of 50% indicates a sample size of 8 mice for each study gro p and for each protocol. Based on these estimates it is estimated that a total of approximately 192 mice (3 aptamers x 4 time points x 2 doses) are needed to complete the study. Prior to collection of tissues, the mice are euthanized by a barbiturate overdose.
Humans: Healthy individuals (>18 years of age, 50% males) who are not pregnant are recruited from laboratory personnel to donate venous blood for the isolation of platelets. The population recruited reflects the race and etlinic composition of the community. The potential risks of phlebotomy are limited to local pain and local hematoma. Multiple experimental groups are studied from a single 10 ml blood draw.
Aptamer Portion
The present technology uses nucleic acid aptamer constructs that have been created to specifically inhibit the human H3 and H4 hi. stones, in certain embodiments, the constructs are chemically modified (2'-fluoropyridines) RNA constructs making them nuclease resistant. See WO 2010/01 446, which is incorporated by reference in its entirety, in certain embodiments the aptamers are RNA, RNA aptamers have the following characteristics: (1) RNA aptamer libraries have a higher structural complexity as compared to DNA aptamer libraries (most DNA aptamers form predictable and similar G-quadrupkx structures), thereby- increasing the likelihood of identifyin aptamers with the desired properties; and (2) RNA chemistries to increase serum stability and safety have been more extensivel evaluated for in vivo applications. In certain embodiments, DNA aptamers are used. Aptamers are single stranded oligonucleotides that can natural !y fold into different 3- dimensional structures, which have the capability of binding specifically to biosurfaces, a target compound or a moiety. The term "conformational change" refers to the process by which a nucleic acid, such as an. aptamer, adopts a different secondary or tertiary structure. The term "fold" may be substituted for conformational change,
Aptamers have advantages over more traditional affinity molecules such as antibodies in that they are very" stable, can be easily synthesized, and can be chemically manipulated with relative ease. Aptamer synthesis is potentially far cheaper and reproducible than antibody-based diagnostic tests. Aptamers are produced by solid phase chemical synthesis, an accurate and reproducible process with consistency among production batches. An aptamer can be produced in large quantities b polymerase chain reaction .(PGR) and once the sequence is known, can be assembled from individual naturally occurring nucleotides and/or synthetic nucleotides, Aptamers are stable to long-term storage at room temperature, and, if denatured., aptamers can easily be renatured, a feature not shared by antibodies. Furthermore, aptamers have the potential to measure concentrations of ligand in orders of magnitude lower (parts per trillion, or even quadrillion) than those antibody-based diagnostic tests. These characteristics of aptamers make them attractive for diagnostic applications.
Aptamers are typically oligonucleotides that may be single stranded
oligodeoxynucleotides, oligoribonucleotides, or modified oligodeoxynucleotide or oligoribonucleotides. The term "modified" encompasses nucleotides with a eovalent!y modified base and/or sugar. For example, modified nucleotides include nucleotide having sugars which are covalently attached to low molecular weight organic groups other than a hydroxy! group at the 3' position and other than a phosphate group at the 5' position. Thus modified nucleotides may also include 2' substituted sugars such as 2'-0-methyl~; 2-O-alkyl; 2-O-allyl; 2 -S-alkyI; 2'-S-allyl; 2 -fluoro-; 2.'-halo or 2-azido-ribose, carbocyclic sugar analogues a-anomeric sugars; epimeric sugars such as arabinose, xyloses or lyxoses, pyranose sugars, furanose sugars, and sedoheptulose.
Modified nucleotides are known in the art and include, by example and not by way of limitation, alkylated purines and/or pyrimidines; acy!ated purines and/or pyrimidines; or other heterocycles. These classes of pyrimidines and purines are known in the art and include, pseudoisocytosine; N4, N4-ethatiocytosine; 8-hydroxy-N6-methyladenine; 4- acetyJcytosine, 5~(carbox.yhydroxylmethyl) uracil; 5-fluorouracil.; 5-bromouracil; 5~ carboxymethylaminomethyl-2-thiouracil; 5-carboxymethylaminomethyl uracil;
dihydrouracil; inosfne; N6-isopentyl-adenine: l-methyladenine; 1 -methylpseudOuracil; 1 - methyl guanine; 2,2-dimeihyl guanine; 2-methylademne; 2-inethyl.guanme; 3-methylcytosine: 5-rnethyleytosine; 6-raethyladenine; 7-methylguanine; 5-methylammomethyl uracil; 5- methoxy amino methy i -2 -thi ouraci ] ; β-D-mamiosylqueosine; 5- methoxycarbon Imethyluracil; 5-methoxyuracil; 2~memy^tjhio-N6-isopentenyladettine;
uracil-5-oxyacetic acid methyl ester; psueouraciJ; 2-thiocytosine; 5-methyl-2 thiouraeih 2- thiouracil; 4-thio uracil; 5-methyluracil; N-uracil-5-oxyacetic acid methylesier; uracil 5- oxyacetic acid; queosine; 2 hiocytosme; 5 -propyl uracil; 5-propyl.cytositie; 5-ethyluracil; 5- ethylcytosine; 5-butyluracil; 5-pentyluracil; 5-pentyleytosine; and 2,6,-diaminopurine;
methylpsuedoufacil; l-rnethylguanine; 1 -methylcytosine.
n certain embodiments, aptame modifications include: (a) Nucleotides modified by replacing the 2' position with either a fiuoro- (F),. amino- (NH2) or O-roethyl {OCH3) group for enhanced nuclease resistance. These modified nucleotides are introduced either chemically or enzymatically. (b) Bridging phosphorothioates incorporated enzymatically. (c) End caps that involve reversing the polarity of the chain incorporated during chemical synthesis, (d) Linkers inserted at the 5 '-ends of aptarners by either chemical or enzymatic means to provide handles for conjugation or to alter pharmacokinetic properties. Keefe et al, Aptarners as therapeutics, Nat Rev Drug Discov. 2010 Jui;9(7):537-50.
In certain embodiments, modification of the '-position of the ribose indirectly improves nuclease resistance of the mternucleotide phosphate bond and at the same time increases duplex stability (Tm), and also provides protection from immune activation. 2'-0- methyl RNA (2'OMe) is a naturally occurring RNA variant found in mammalian ribosomal RNAs and transfer RNAs. it is nontoxic and can be placed within either the S or AS strands of a si RNA. Heavy modification with 2'OMe RNA can reduce potency or completely inactivate a siRNA; alternating 2'OMe with RNA bases generally retains siRNA function while conferring significant nuclease stabilization. 2'OMe RNA can be combined with other 2 '-modifications which are not naturally occurring bases with good results. Behlke, Chemical Modification of 'siRNAs for in Vivo Use, Oligonucleotides 18:305-320 (2008).
The 2'-iluoro (2'-F) modification is compatible with siRNA function and also helps stabilize the duplex against nuclease degradation. Incorporatio of 2'-F at pyrirnidine positions maintains siRNA activity in vitro and in vivo. The 2 -F modification is even tolerated at the site of Ago2 cleavage. The combined use of 2 -F pyrimidines with 2'OMe purines can results in RNA duplexes with extreme stability in serum and improved in vivo performance. The 2'-0-(2-methoxyethyI) RNA (MOE) modification has been extensively used in anti sense oligonucleotides and confers significant nuclease stability to an. oligonucleotide as well as increases Tm. 2'- OE residues can be incorporated into siRNAs much like 2'OMe or 2'~F, however this modification is not generally available for use. The 2'- fluoro- -D-arabinonucieotide (FANA) modification has also shown promise in antisense oligonucleotide applications and can also be placed in siRNAs, Substitution of FANA for RNA in the entire S-strand confers significant stabilization to nucleases while maintaining functional potency of the duplex; however, the AS-strand is less tolerant of the FANA modification. Locked nucleic acids (LNAs) contain a methylene bridge which connects the 2'-0 with the 4'-C of the ribose. The methylene bridge 'locks" the sugar in the 3'-endo conformation, providing both a significant increase in Tm as well as nuclease resistance. Extensive modification of a siRNA with LNA bases generally results in decreased activity (even more so than 2'OMe); however, siRNAs with limited incorporation retain functionalit and offer significant nuclease stabilization.
The aptamers of the invention are synthesized using conventional phosphodiester linked nucleotides and synthesized usin standard solid or solution phase synthesis techniques which are known in the art. Linkages between nucleotides may use alternative linking molecules. For example, linking groups of the formula P(0)S, (thioate); P(S)S, (dithioatc); P(0)NR'2; P(C))R'; P(0)OR6; CO; or CONR'2 wherein R is H (or a salt) or alkyl (1 -12C) and R6 is alkyl (1-9C) is joined to adjacent nucleotides through— 0-- or— S— .
Certain embodiments of the present invention provide a nucleic acid molecule of not more than 90 nucleotides in length comprising an aptamer, wherein the aptamer specifically targets extracelluiar histones. In certain embodiments of the present invention, the aptamer portion is specific for human histones H3 and/or H4.
In certain embodiments, the aptamer comprises at least 90% identity to an RNA sequence listed in Table 1. In certain embodiments, the aptamer comprises at least 95% identity to an RNA sequence listed in Table I . In certain embodiments, the aptamer comprises 100% identity to an RNA sequence listed in Table 1.
In certain embodiments, the aptamer specifically targets human H3 and 114, In certain embodiments, the aptamer comprises at least 90% identity to KU1 , KIJ2 or U3. In certain embodiments, the aptamer comprises at least 95 identity to KU1 , KU2 or KU3. In certain embodiments, the aptamer has 100% identity to KU 1 , KU2 or KU3.
In certain embodiments, the aptamer specifically targets human H3. In certain embodiments, the e aptamer comprises at least 90% identity to IJ7, KUS or U9. In certain embodiments, the aptamer comprises at least 95% identity to K.U7, KU8 or TJ9, In certain embodiments, the aptamer has 100% identity to U7, KU or KU9. in certain embodiments, the aptamer specifically targets human H4. In certain embodiments, the aptamer comprises at least 90% identity to KU4, U5 or KU6. In certain embodiments, the aptamer comprises at least 95% identity to U4, KU5 or U6. In certain embodiments, the aptamer has 100% identity to KU4, KU5 or KU6.
In certain embodiments, additional modifications are made to the aptamer portion. Additional modifications to the aptamer portion include 2'0-methyi modification of the pyrimidines. In other embodiments, all of the nucleotides in the aptamer are 2'0-meth l modified. Alternatively, the pyrimidines, or all the nucleotides, may be modified with 2'fluoros (both pyrimidines and purines). Additional modifications to the nucleotides in the aptamer include large molecular weight conjugates like PEGy!ation, lipid-based
modifications (e.g., cholesterol) or nanoparticles (e.g., PET or chitosan) to improve the pharmac k i neti c d y n ami c profile of the chimera.
Small Molecule Portion
In certain embodiments, the aptamers are linked to other molecules in order to improve clearance (pharmacokinetics and pharmacodynamics). In certain embodiments, the other molecules include PEG, cholesterol or other lipid, nanoparticles. The aptamers of the present invention can be operably linked to one or more small molecule entities, in certain embodiments, the entity is a fluorescen tag, affinity tag, a protein, a solid substrate, a cell surface, or a cellular component. In certain embodiments, the cellular component is a cell wall or ceil membrane. In certain embodiments, the solid substrate is a component of si lica, cellulose, cellulose acetate, nitrocellulose, nylon, polyester, polyethersu!fone, polyolefm, or polyvinylidene fluoride, or combinations thereof. In certain embodiments, the solid substrate is a stent or other medical device, filter, magnetic bead, metal oxide, latex particle, microliter plates, polystyrene bead, or CD-ROM.
In certain embodiments, the aptamer i linked to the entity by means of a linker. In certain embodiments, the linker is a binding pair, in certain embodiments, the "binding pair" refers to two molecules which interact with each other through any of a variety of molecular forces including, for example, ionic, covalent, hydrophobic, van der Waals, and hydrogen bonding, so that the pair have the property of binding specifically to each other. Speci fic binding means that the binding pair members exhibit binding to each other under conditions where they do not bind to another molecule. Examples of binding pairs are biotin-avidin, hormone-receptor, receptor-ligand, enzyme-substrate, IgG-protein A, antigen-antibody, and the like. In certain embodiments, a first member of the binding pair comprises avid in or streptavidin and a second member of the binding pair comprises biotin. In certain embodiments, the aptamer is linked to the entity by means of a covalent bond.
The entity, for example, may additionally or alternatively, be a detection means. A number of "molecular beacons" (such as fluorescence compounds) can be attached to aptamers to provide a means for signaling the presence of and quantifying a target chemical or biological agerit. Other exemplary detection labels that could be attached to the aptamers include biotin, any fluorescent dye or tracer, amine modification, horseradish peroxidase, alkaline phosphatase, etc.
in certain embodiments, the aptamer is operably linked to a detection means and to a solid substrate. For example, the aptamer may be linked to a fluorescent dye and to a magnetic bead.
In certain embodiments, the small molecules are biologic or pharmacologic agents that can inhibit H3 and/or H4 liistones,
Linking Molecules
Chemistries thai can be used to link molecules to the aptamer are known in the art, such as disulfide linkages, amino linkages, covalent linkages, etc. Additional linkages and modifications can be found on the world-wide-web at
trilinkbiotech.conv'products/oligo/oHgo modifications.asp,
Non-Aptamer Therapeutic Agents and Diagnostic Labels
Other non-antibody therapeutic agents targeted against either histones or downstream effectors of a histone mediated pathway may also be utilized in combination with anti-histone antibodies or fragments thereof, administered either before, simultaneousl with, or following administration of one or more anti-histone antibodies or fragments thereof. Various therapeutic agents of use in treating histone associated disease states are known in the art, such as activated protein C (APC), thrombomodulin, a peptide fragment of histone i f 1 , Ή2Α, H2B, ϊ 13 or H4, granzyme A, granzyrne B, plasmin, Factor 7-activating protease, heparin, and any such known agent may be utilized in combination with the subject anti-histone antibodies or antibody fragments. A human histone H4 peptide may comprise residues 50-67 or 40-78 of human H4 (see, e.g., U.S. Pub!. No. 200901 1 7099).
In certain embodiments, the therapeutic agent is selected from the group consisting of an antibody, an antibody fragment, an immune conjugate, a radionuclide, an
immunomodulatory, an anti-angiogenic agent, a pro-apopto ie agent, a cytokine, a chemokine, a drug, a toxin, a hormone, an siR A, a cytokine, a chemokine, a coagulation inhibitor, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony stimulating factor, an interferon, erythropoietin, thrombopoietin, an enzyme, recombinant human thrombomodulin and activated human protein C.
In certain embodiments, the antibody, antibody fragment or immune conjugate binds to an antigen selected from the group consisting of histone H2B, histone H3, historic H4. a proinflammatory effector of the innate immune system, a proinilammatory effector cytokine, a proinflammatory effector chemokine, a target specifically associated with infectious disease, acute respiratory distress syndrome, septicemia, septic shock, GVHD, transplant rejection, atherosclerosis, asthma, granulomatous disease, a neuropathy, cachexia, a coagulopathy, acne, giant cell arteritis or myocardial ischemia, TNF-a, MIP, CD74, HLA- DR, IL~J . ! L-3, IL-4, 3L-5, lL-6, IL-8, IL-12, IL-1 5, IL-17, IL-18, IL-23, 1L-4 , IL-6R, JL- 13R, IL- I 5R, IL-17R, 1L-18R, CD40L, CD44, CD46, CD55, CD59, CCL19, ( GU I . mCRP, MCP-1 , MIP-IA, MIP- I B, RANTES, ENA-78, IP- 10, GRO-β, lipopo!ysaceharide, iymphotoxin, HMGB-1, tissue factor, a complement regulatory proiein, a coagulation factor, thrombin, a complement factor, C3, C3a, C3b, C4a, C4b, C5, C5a, C5b, Fit- 1 and VEGF, in certain embodiments, the drug is selected from the group consisting of 5- fmoro uracil, aplidin, azaribine, anastrozole, anthracyclines, bendamustine, bleomycin, bortezomib, bryo statin- 1 , busulfan, calicheamycin, camptothecin, carboplatin, 10- hydroxycamptothecin, carmustine, celebrex, chlorambucil, cisp latin (CDDP), Cox-2 inhibitors, irinotecan (CPT-1 1), SN-38, carboplatin, cladribine, camptotheeans,
cyclophosphamide, cytarabine, daearhazine, docetaxel, dactinomycin, daunorubicin, doxorubicin, 2-pyrrolinodoxorubicine (2P-DOX), cyano-morpholino doxorubicin, doxorubicin glucuroni.de, epirubicin g!ucuronide, esiramustine, epipodophyilotoxin, estrogen receptor binding agents, etoposide (VP 16), etoposide g!ucuronide, etoposide phosphate, tloxuridine (FUdR), 3',5'-0-dioleoyI~FudR (FUdR-dO), fJudarabine, flutamide, farnesyi- protein transferase inhibitors, gemcitabine, hydroxyurea, idaruhicin, ifosfamide, L- asparaginase, lenolidamide, lcucovorin. lomustine, mechiorethamine, melphalan,
mercaptopurine, 6-mercaptopurine, methotrexate, mitoxantrone, mithramycin, mitomycin, mitotane, navelbine, nitrosourea, plieomycin, procarbazine, paeiitaxel, peniostatin, PSi-341, raloxifene, semustine, streptozocin, tamoxifen, taxol, temazolomide (an aqueous form of DTIG), transplatinum, thalidomide, ihioguanuie, thiotepa, teniposicle, topotecan, uracil mustard, vinoreibine, vinblastine, vincristine, a vinca alkaloid, a tyrophostin, canertinib, dasatinib, erlotinib. gefitinib, imatinib, lapatinib, lellunomide. nilotinib, pazopanib, semaxinib, sorafenib, sunitinib, sutent, vatalanib, PC.!-32765 (ibrutinib), PCI-45292, GDC- 0834, LFM-A13 and RN4S6. In certain embodiments, the toxin is selected from the group consisting of ricin. abrin. alpha toxin, saporin, ribonuclease (RNase). e.g., onconase, DNase I, Staphylococcal enterotoxin-A. poke weed antiviral protein, gelomn, diphtheria toxin, Pseudamonas exotoxin, and Pseudomonas endotoxin.
In. certain embodiments, the immunomodulatory is selected from the group consisting of a cytokine, a stem cell growth factor, a lymphotoxin, a hematopoietic factor, a colony stimulating factor (CSF), an interleukin (IL), erythropoietin, thrombopoieiin, tumor necrosis factor (TNF), granulocyie-cofony stimulating factor (G-CS.F), granulocyte, macrophage- colony stimulating factor (G -CSF), interferon-a, interferon-β, interferon-γ, interferon-!. TGF-a, TGF-β, interleukin- 1 (IL-1), IL-la,. IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 10, IL-1 1 , IL-12; IL-I3, IL-14, IL-15, IL-16, IL-1.7, lL-I-8, 11-2) , IL-23, ! 1.-25. L1.F, FLT-3, angiostattn, thrombospondin, recombinant human thrombomodulin- endostatin and lymphotoxin.
In certain embodiments, the cytokine is selected from the group consisting of human growth honnone, N-meihionyl human growth hormone, bovine growth hormone, parathyroid hormone, thyroxine, insulin, proinsulin, relaxin, prorelaxin. follicle stimulating honnone (FSH), thyroid stimulating hormone (TSH), luteinizing hormone (LH), hepatic growth factor, prostaglandin, fibroblast growth factor, prolactin, placental lactogen, OR protein, tumor necrosis factor-a, tumor necrosis factor- β, mulierian-inhibiting substance, mouse
gonadotropm-associated peptide, inhibin, activin, vascular endothelial growth factor, integrin, thrombopoieiin (TPO), NGF-fl platelet-growth, factor, TGF-a, TGF-β, insulin-like growth factor-], insulin-like growth factor-II, erythropoietin (EPO), osteoinductive factors, interferon-a, interferon-β, interferon -γ. macrophage-CSF (M-CSF), IL-1 , IL-l a, 11 -2, IL-3, IL-4, IL-5, IL-6, IL-7, 1L-8, IL-9, IL-10, IL-1 1 , IL-12, f i - 13. IL-14, IL- 1 5, IL-16, ii - 17, IL- 18, l.L-21 , IL-25, LIF, FLT-3, angiostatin, thrombospondin, endostatin, tumor necrosis factor and lymphotoxin.
In certain embodiments, the radionuclide is selected from the group consisting of mIn, 1 HAt, mLu, 2nBi, 212Bi, 2!3Bi, 21 'At, 62Cu, 67Cu, 9% 125I, ml, i r% 32P, ¾ 47Sc, u ,Ag, 67Ga, i i3Sm, ,6,Tb, ,53Dy, mOy, 61Ho, !66I!o, !86Re, mRe, i¾)Re, 2i,Pb, mPb, 223Ra, 2 5Ac, 77As, ¾r, "Mo, l05Ru, 549Pm, ,69Er, mlr, 5¾Co, 80mBr, 99mTc, 103mRh, 10 Pt ! ,9Sb, 125L , s¾f!Os, !9 Ir, 219Rn, 215Po, 22f Fr, 25SFm. UC, , 3N, ¾ 75Br, mAu, 199Au, 224Ac, 77Br, i i3mIn, 95Ru, 97Ru, 103Ru, !05Ru, 107Hg, 03Hg, ,2f mTe, i22mTe, i 2 f"Te, ¾, 1<¾7Tm, 168Tm, ,97Pt, 109Pd, J42Pr, ]43Pr, i61Tb, 57Co, 5SCo, 51Cr, 59Fe, 75 Se, 201T1, 76Br, i 9Yb and 2 7Th. Amplification Methods
In one embodiment of the present invention, the method involves the amplification of selected RNAs. "Amplifying'' utilizes methods such as the polymerase chain reaction (PGR), ligation amplification (or ligase chain reaction, LCR), strand displacement amplification, nucleic acid sequence-based amplification, and amplification methods based on the use of Q- beta replicase. These methods are well known and widely practiced in the art, Reagents and hardware for conducting PCR are commercially available. In one embodiment of the present invention, at leasl one type of aptamer is immobilized on a solid surface.
According to the methods of the present invention, the amplification may be carried out by any means known to the art. Examples of suitable amplification techniques include, but are not limited to, polymerase chain reaction (including, for RNA amplification, reverse- transcriptase polymerase chain reaction), ligase chain reaction, strand displacement amplification, transcription-based amplification, self-sustained sequence replication (or '"3SR,;), the Qp replicase system, nucleic acid sequence-based amplification (or "NASBA"), the repair chain reaction (or "RCR"), and boomerang DNA amplification (o "BDA").
'fhe bases incorporated into the amplification product may be natural or modified bases (modified before or after amplification), and the bases may be selected to optimize subsequent electrochemical detection steps.
Polymerase chain reaction (PCR) may be carried out in accordance with known techniques. See, e.g., U.S. Patent Numbers 4,683, 395; 4.683,202; 4,800,159; and 4,965,188. in general, PCR involves, first, treating a nucleic acid sample (e.g., in the presence of a heat stable DNA polymerase) with one oligonucleotide primer for each strand of the specific sequence to be detected under hybridizing conditions so that an extension product of each primer is synthesized that is complementary to each nucleic acid strand, with the primers sufficiently complementary to each strand of fhe specific sequence to hybridize therewith so that the extension product, synthesized from each primer, when it is separated from its complement, can serve as a template for synthesis of the extension product of the other primer, and then treating the sample under denaturing conditions to separate the primer extension products from their templates if the sequence or sequences to be detected are present. These steps are cyclically repeated until the desired degree of amplification is obtained. Detection of the amplified sequence may be can ied out by adding to the reaction product an oligonucleotide probe capable of hybridizing to the reaction product (e.g.,. an oligonucleotide probe of the present invention), the probe carrying a detectable label, and then detecting the label in accordance with known techniques. Where the nucleic acid to be amplified is A, ampiification may be carried out by initial conversion to DNA by reverse transcriptase in accordance with known techniques.
Strand displacement ampiification (SDA) may be carried out in accordance with known techniques. For example. SDA may be earned out with a single amplification primer or a pair of amplification primers, with exponential amplification, being achieved with the latter. In general, SDA amplification primers comprise, in the 5' to 3' direction, a flanking, sequence (the DNA sequence of which is noncritical ), a restriction site for the restriction enzyme employed in the reaction, and an oligonucleotide sequence (e.g., an oligonucleotide probe of the present invention) that hybridizes to the target sequence to be amplified and/or detected. The flanking sequence, which serves t facilitate binding of the restriction enzyme to the recognition site and provides a DNA polymerase priming site after the restriction site has been nicked, is about 15 to 20 nucleotides in length in one embodiment. The restriction site is functional in the SDA reaction. The oligonucleotide probe portion is about 13 to 15 nucleotides in length in one embodiment of the invention.
Ligase chain reaction (LCR) is also carried out in accordance with known techniques, in general, the reaction is carried out with two pairs of oligonucleotide probes: one pair binds to one strand of the sequence to be detected; the other pair binds to the other strand of the sequence to be detected. Each pair together completely overlaps the strand to which it corresponds. The reaction is carried out by, first, denaturing (e.g., separating) the strands of the sequence to be detected, then reacting the strands wi th the two pairs of oligonucleotide probes in the presence of a heat stable ligase so that each pair of oligonucleotide probes is ligated together, then separating the reaction product, and then cyclically repeating the process until the sequence has been amplified t the desired degree. Detection may then be carried out in like manner as described above with respect to PCR.
Diagnostic techniques that are useful in the methods of the invention include, but are not limited to direct DNA sequencing, pulsed-field gel electrophoresis (PFGE) analysis, allele-specific oligonucleotide (A SO), dot blot analysis and denaturing gradient gel electrophoresis, and are well known to the artisan.
The sample may be contacted with the aptamer in any suitable manner known to those skilled in the art. For example, the sample may be solubilized in solution, and contacted with the aptamer by solubilizing the aptamer in solution with the sample under conditions that permit binding. Suitable conditions are well known to those skilled in the art. Alternatively, the sample may be solubilized in solution with the aptamer immobilized on a solid support, whereby the sample may be contacted with the a tamer by immersing the solid support, having the aptamer immobilized thereon in the solution containing the sample.
General Term ino logy
"Synthetic" aptamers are those prepared by chemical synthesis. The aptamers may also be produced by recombinant nucleic acid methods. "Recombinant nucleic molecule" is a combination of nucleic sequences that are joined together using recombinant nucleic technology and procedures used to join together nucleic sequences known in the art.
A "therapeutic agent' is an atom, molecule, or compound that is useful in the treatment of a disease. Examples of therapeutic agents include antibodies, antibody fragments, peptides, drugs, toxins, enzymes, nucleases, hormones, immune modulators, anti sense oligonucleotides, small interfering RNA (siRNA), chelators, boron compounds, photoactive agents, dyes,, and radioisotopes. An "antibody" as used herein refers to a full- length (i.e., naturally occurring or formed by normal immunoglobulin gene fragment recombinatorial processes) immunoglobulin molecule (e.g., an IgG antibody). An "antibody" includes monoclonal, polyclonal, bispecific, multispecific. murine, chimeric, humanized and human antibodies. An "antibod fragment' ' is a portion of an intact antibody such as F(ab')2, F(ab)2, Fab', Fab, Fv, st, scFv, dAb and the like. Regardless of structure, an antibody fragment binds with the same antigen that is recognized by the full length antibody. For example, antibody fragments include isolated fragments consisting of the variable regions, such as the "Fv" fragments consisting of the variable regions of the heavy and light chains or recombinant single chain polypeptide molecules in which light and heavy variable regions are connected by a peptide linker ("scFv proteins"'). "Single chain antibodies", often abbreviated as "scFv" consist of a polypeptide chain that comprises both a VH and a V; domain which interact to form an. antigen-binding site. The VH andVi, domains are usually linked by a peptide of 1 to 25 amino acid residues. Antibody fragments also include diabodies, triabodies and single domain antibodies (dAb), Fragments of antibodies that do not bind to the same antigen as the intact antibody, such as the Fc fragment, are not included within the scope of an "antibody fragment" as used herein. In certain embodiments, the antibody is an anri- histone antibody (see., e.g., US 2014/0234209, which is incorporated by reference herein).
A "diagnostic agent*' is an atom, molecule, or compound that is useful in diagnosing a disease. Useful diagnostic agents include, but are not limited to, radioisotopes, dyes (such as with the biotin-streptavidm complex), contras agents, fluorescent compounds or molecules, and enhancing agents (e.g., paramagnetic ions) for magnetic resonance imaging (MR1). An anii-histone aptamer, antibody or antibody fragment, or a composition described herein, is said to be admi istered in a "therapeutically effective amount'' if the amount administered is physiologically significant. An agent is physiologically significant if its presence results in a detectable change in the physiology of a recipient subject. In particular embodiments, an aptamer, antibody or antibody fragment preparation is physiologically significant if its presence invokes an antitumor response or mitigates the signs and symptoms of an autoimmune disease state. A physiologically significant effect could also be the evocation of a humoral and/or cellular immune response in the recipient subject leading to growth inhibition or death of target cells.
The term "chimeric" refers to a gene or DNA that contains 1 ) DMA sequences, including regulatory and coding sequences that are not found together in nature or 2) sequences encoding parts of proteins not naturally adjoined, or 3) parts of promoters that are hot naturally adjoined. Accordingly, a chimeric gene may include regulatory sequences and coding sequences that are derived from different sources, or include regulatory sequences and coding sequences derived from the same source, but arranged in a manner different from that found in nature.
As used herein, the term "nucleic acid" and "polynucleotide" refers to
deoxyriboftucleotid.es or ribonucleotides and polymers thereof in either single- or double- stranded form, composed of monomers (nucleotides) containing a sugar, phosphate and a base that is either a purine or pyrimidine. Unless specifically limited, the term encompasses nucleic acids containing known analogs of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring, nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g , -degenerate codon substitutions) and. complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third, position of one or more selected f or all) codons is substituted with mixed- base and/or deoxyinosine residues.
A "nucleic acid fragment" is a portion of a given nucleic acid molecule.
Deoxyribonucleic acid (DNA) in the majority of organisms is the genetic materia! while ribonucleic acid (RNA) is involved in the transfer of information contained within DNA into proteins. The term "nucleotide sequence"' refers to a polymer of DNA or RNA which can be single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases capable of incorporation into DNA or RNA polymers. The terms "nucleic acid," "nucleic acid molecule/* "nucleic acid fragment," "nucleic acid sequence or segment," or "polynucleotide" may also be used interchangeably with gene, cDNA, DNA and RNA encoded by a gene, e.g., genomic DNA, and even synthetic DNA sequences. The term also includes sequences (hat include any of the known base analogs of DNA and RNA.
By ''fragment" or "portion" is meant a full length or less than full length of the nucleotide sequence.
A "variant" of a molecule is a sequence that is substantially similar to the sequence of the native molecule. For nucleotide sequences, variants include those sequences that, because of the degeneracy of the genetic code, encode the identical amino acid sequence of the native protein. Nciturally occurring allelic variants such as these can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PGR) and hybridization techniques. Variant nucleotide sequences also include syntheticall derived nucleotide sequences, such as those generated, for example, by using site -directed mutagenesis that encode the native protein, as well as those that encode a polypeptide having amino acid substitutions. Generally, nucieotidc sequence variants of the invention will have in at least one embodiment 40%. 50%, 60%, to 70%, e.g., 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%. to 79%. generally at least 80%, e.g. , 81%-84%, at least 85%, e.g., 86%, 87%. 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98%, sequence identity to the native (endogenous) nucleotide sequence.
As used herein, "sequence identity" or 'identity" in the context of two nucleic acid sequences makes re ference to a specified percentage of residues in the tw o sequences that are the same when aligned by sequence comparison algorithms or by visual inspection.
As used herein, "percentage of sequence identity" means the value determined by comparing two optimally aligned sequences, wherein the portion of the polynucleotide sequence may comprise additions or deletions {i.e., gaps) as compared to the reference sequence ( which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity.
The term "substantial identity" of polynucleotide sequences means that a
polynucleotide comprises a sequence that has at least 70%, 71%, 7.2%, 73%, 74%, 75%, 76%. 77%, 78% or 79%; at least 80%,, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, or 89%; at least 90%, 91%, 92%, 93%, or 94%; or even at least 95%, 96%, 97%, 98%, or 99% sequence identity,: compared to a reference sequence using one of the alignment programs described using standard parameters.
Another indication that nucleotide sequences are substantially identical is if two molecules hybridize to each other under stringent conditions (see below). Generally, stringent conditions are selected to be about 5°C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. However, stringent conditions encompass temperatures in the range of about PC to about 20°C, depending upon the desired degree of stringency as otherwise qualified herein. Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the polypeptides they encode are substantially identical. This may occur, e.g., when a copy of a nucleic acid is created using the maximum, codon degeneracy permitted by the genetic code.
As noted above, another indication that two nucleic acid sequences are substantially identical is that the two molecules hybridize to each other under stringent conditions. The phrase "hybridizing specifically to" refers to tine binding, duplexing, or hybridizing of a molecule only to a particular nucleotide sequence under stringent conditions when that sequence is present in a complex mixture (e.g., total cellular) DNA or RN A. "Bmd(s) substantially" refers to complementary hybridization between a probe nucleic acid and a target nucleic acid and embraces minor mismatches that can be accommodated b reducing the stringency of the hybridization media to achieve the desired detection of the target nucleic acid sequence.
'"Stringent hybridization conditions" and "stringent hybridization wash conditions" in the context of nucleic acid hybridization experiments such as Southern and Northern hybridizations are sequence dependent, and are different under different environmental parameters. Longer sequences hybridize specifically at higher temperatures. The "!',.. is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched nucleic acid. Specificity is typically the function of post- hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. For DNA-DNA hybrids, the Tm can be approximated from, the equation of Meinkoth and Wahh Tra 81.5°C + 16.6 (log M) +0.41 (%GC) - 0.61 (% form) - 500/1,. M is the molarity of monovalent cations, %GC is the percentage of guanosine- and cytosine nucleotides in the DN A, % form is the percentage of formamide in the hybridization solution, and L is the length of the hybrid in base pairs. Tra is reduced by about 1 °C for each 1 % of mismatching; thus, Tm, hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with >90% identity are sought, the T,„ can be decreased 1 G°C. Generally, stringent conditions are selected to be about 5°C lower than the thermal melting point (Tm) for the specific sequence and its complement at a defined ionic strength and pi L However, severely stringent conditions can utilize a hybridization and/or wash at 1 , 2. 3, or 4°C lower than the thermal melting point (Tm);
moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9, or 10°C lower than the thermal melting point (Tm); low stringency conditions can utilize a
hybridization and/or wash at 1 1, 12, 13, 14, 15, or 20°C lower than the thermal melting point (Tm)- Using the equation, hybridization and wash compositions, and desired T, those of ordinary skill will, understand that variations m the stringency of hybridization and/or wash solutions are inherently described. If the desired degree of mismatching results in a T of less than 45°C (aqueous solution) or 32°C (formamide solution), i is preferred to increase the SSC concentration so that a higher temperature can be used. Generally, highly stringent hybridization and wash conditions are selected to be about 5°C lower than the thermal melting point (TTO) for the specific sequence at a defined ionic strength and pH.
An example of highly stringent wash conditions is 0. 5 M NaCl at 72°C for about 15 minutes. A example of stringent wash conditions is a 0.2X SSC wash at 65 °C for 15 minutes. Often, a high stringency wash is preceded by a low stringency wash to remove background probe signal. An example medium stringency wash for a duplex of, e.g, more than 100 nucleotides, is IX SSC at 45°C for 15 minutes. An example low stringency wash for a duplex of, e.g., more than 100 nucleotides, is 4-6X SSC at 40°C for 15 minutes. For short probes (e.g., about 10 to 50 nucleotides), stringent conditions typically involve sal concentrations of less than about L5 M, more preferably about 0.01 to 1.0 M, Na ion concentration (or other salts) at. pH 7.0 to 8,3, and the temperature is typically at least about 30°C and at least about 60CC for long probes (e.g., >50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide, in generaL a signal to noise ratio of 2X (or higher) than that observed for an mirelated probe in the particular hybridization assay indicates detection of a specific hybridization.
Very stringent conditions are selected to be equal to the Tra for a particular probe. An example of stringent conditions for hybridization of complementar nucleic acids which have more than 100 complementary residues on a filter in a Southern or Northern blot is 50% formamide, e.g., hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37°C, and a wash in 0.1 X SSC at 60 to 65°C. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1M NaCl, 1% SDS (sodium dodecyl sulphate) at 37ΉΛ and a wash in I X to 2X SSC (20X SSC = 3.0 M NaCl/0.3 M tri sodium citrate) at 50 to 55°C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 .0 M NaCl, 1% SDS at 37°C, and a wash in 0.5X to IX SSC at 55 to 60°C.
"Operably-linked'' nucleic acids refers to the association of nucleic acid sequences on single nucleic acid fragment so that the function of one is affected by the other, e.g., an arrangement of elements wherein the components so described are configured so as to perform their usual function. For example, a regulatory DNA sequence is said to be
"operab!y linked to" or "associated with"' a DNA sequence that codes for an NA or a polypeptide if the two sequences are situated such that the regulatory DNA sequence affects expression of the coding DNA sequence (i.e., that the coding sequence or functional RNA is under the transcriptional control of the promoter). Coding sequences can be operably-linked to regulatory sequences in sense or antisense orientation. Control elements operably linked to a coding sequence are capable of effecting the expression of the coding sequence. The control elements need not be contiguous with the coding sequence, so long as they function to direct the expression thereof. Thus, for example, intervening untranslated yet transcribed sequences can be present between a promoter and the coding sequence and the promoter ca still be considered "operably linked" to the coding sequence.
The terms "isolated and/or purified" refer to in vitro isolation of a. nucleic acid, e.g. , a DNA or RNA molecule from its natural cellular- environment, and from association with other components of the cell or test solution (e.g. RNA pool), such as nucleic acid or polypeptide, so that it can be sequenced, replicated, and/or expressed. For example, "isolated nucleic acid" may be a DNA molecule containing less than 31 sequential nucleotides that is transcribed into an RNAi molecule. Such an isolated RNAi molecule may, for example, for a hairpin structure with a duplex 21 base pairs in length that is complementary or hybridizes to a sequence in a gene of interest, and remains stably bound under stringent conditions (as defined by methods well known in the art, e.g., in Sambrook and Russell, 2001). Thus, the RNA or DNA is "isolated" in that it is free from at least one contaminating nucleic acid with which it is normally associated in the natural source of the RNA or DNA and is preferably substantially free of any other mammalia RNA or DNA, The phrase "free from at least one. contaminating source nucleic acid with which it is normally associated" includes the case where the nucleic acid is reintroduced into the source or natural cell but is in a different chromosomal location or is otherwise flanked by nucleic acid sequences not normally found in the source cell, e.g.. in a vector or plasmid. Nucleic acid molecules having base substitutions (i.e., variants) are prepared by a variety of methods known in the art. These methods include, but are not limited to, isolation from a natural source (in the case of natural ly occurring sequence variants) or preparation by oMgonucIeotide-mediated (or site-directed) mutagenesis, PCR mutagenesis, and cassette mutagenesis of an. earlier prepared variant or a non-variant version of the nucleic acid molecule.
"Operably- linked'' refers to the association of nucleic acid sequences on single nucleic acid fragment so that the function of one of the sequences is affected by another. For example, a regulatory DNA sequence is said to be "operably linked to" or "associated with" a DNA sequence that codes for an RNA or a polypeptide if the two sequences are situated such that the regulatory DNA sequence affects expression of the coding D A sequence (i.e., that the coding sequence or functional RNA is under the transcriptional control of the promoter). Coding sequences can be operably-linked to regulatory sequences in sense or antisense orientation,
As used herein, the term "derived" or "directed to" with respect to a nucleotide molecule means that the molecule has complementary sequence identity to a particular ■molecule of interest,
"Treating" as used herein refers to ameliorating at least one symptom of, curing and/or preventing the development of a disease or a condition,
The terms "protein," "peptide" and "polypeptide" are used interchangeably herein.
Dosages, Formulations and Routes of Administration of the Agents of the Invention The agents of the invention are preferably administered so as to result in a reduction in at least one symptom associated with a disease. The amount administered will vary depending on various factors including, but. not limited to, the composition chosen, the particular disease, the weight, the physical condition, and the age of the mammal, and whether prevention or treatment is to be achieved. Such factors can be readily determined by the clinician employing animal models or other test systems, which are well known to the art.
Administration of the aptamer chimera may be accomplished through the
administration of the nuc leic acid molecule. Pharmaceutical formulations, dosages and routes of administration for nucleic acids are generally known in the art.
The present invention envisions treating a disease, for example, vascular stenosis, restenosis or stroke, in a mammal by the administration of an agent, e.g., a nucleic acid composition, an expression vector, or a viral particle of the invention. Administration of the therapeutic agents in accordance with the present invention may be continuous or Intermittent, depending, for example, upon the recipient's physiological condition, whether the purpose of the administration is therapeutic or prophylactic, and other factors known to skilled practitioners. The administration of the agents of the invention may be essentially continuous over a preselected period of time or may be in a series of spaced doses. Both local and systemic administration is contemplated.
One or more suitable unit dosage forms having the therapeutic agent(s) of the invention, which, as discussed, below, may optionally be formulated for sustained release (for example using microencapsulation), can be administered by a variety of routes including parenteral, including by intravenous and intramuscular routes, as well as by direct injection into the diseased tissue. The formulations may, where appropriate, be conveniently presented in discrete unit dosage forms and may be prepared by any of the methods well known to pharmacy* Such methods may include the step of bringing into association the therapeutic agent with liquid carriers, solid matrices, semi-solid carriers, finely divided solid carriers or combinations thereof, and then, if necessary, introducing or shaping the product into the desired delivery system.
When the therapeutic agents of the invention are prepared for administration, they are preferably combined with a pharmaceutically acceptable carrier, diluent or excipient to form a pharmaceutical formulation, or unit dosage form. The total acti ve ingredients in such formulations include from 0.1 to 99.9% by weight of the formulation. A "pharmaceutically acceptable" is a carrier, diluent, excipient, and/or salt that is compatible with the other ingredients of the formulation, and not deleterious to the recipient thereof. The active ingredient for administration may be present as a powder or as granules, as solution, a suspension or an emulsion.
Pharmaceutical formulations containing the therapeutic agents of the invention can be prepared by procedures known in the art using well known and readil available ingredients. The therapeutic agents of the in venti on can also be formulated as solutions a ppropriate for parenteral administration, for instance by intramuscular, subcutaneous or intravenous routes.
The pharmaceutical formulations of the therapeutic agents of the invention can also take the form of an aqueous or anhydrous solution or dispersion, or alternatively the form of an emulsion or suspension.
Thus, the therapeutic agent may be formulated for parenteral administration (e.g., by injection, for example, bolus injection or continuous infusion) and may be presented in unit dose form in ampules, pre-filled syringes, small volume infusion containers or in multi-dose containers with an added preservative. The active ingredients may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredients may be in powder form, obtained by aseptic isolation of sterile solid or by lyophilization from solution, for constitution with a suitable vehicle, e.g. , sterile, pyrogen- free water, before use.
it will be appreciated that the unit content of active ingredient or ingredients contained in an indi vidual aerosol dose of each dosage form need not in itself constitute an effective amount for treating the particular indication or disease since the necessary effective amount can be reached by administration of a plurality of dosage units. Moreover, the effective amount may be achieved using less than the dose in the dosage form, either individually, or in a series of administrations.
The pharmaceutical formulations of the present invention may include, as optional ingredients, pharmaceutically acceptable carriers, diluents, solubilizing or emulsifying agents, and. salts of the type that are well-known in the art. Specific non-limiting examples of the carriers and/or diluents that are useful in the pharmaceutical formulations of the present invention include water and physiologically acceptable buffered saline solutions such as phosphate buffered saline solutions pH. 7,0-8.0, saline solutions, and water.
Example 1
The incidence of multiple organ dysfunction syndrome (MODS) and the related acute respiratory distress syndrome (ARDS) in critically ill patients is as high as 25% with a mortality of more than 40%. Those patients that do survive often have significant morbidity and. prolonged hospitalization and the associated financial burdens. There are several etiologies of MODS/ARDS including trauma, sepsis, burns, ischemia/reperfusion, and acute lung injur (ALI). However, even with identification of high risk patients, there are no effective treatments to prevent the development of these syndromes.
The hypothesis that extracellular h stones mediate tissue injury is a relatively new concept. Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response. Their potential to amplify tissue injury by killing other cells, in addition to their agonistic activity on T LRs, provides strong rationale to target histones for therapy. Since histones (cationic proteins) normally associate with DNA in the nucleosome, oligonucleotides (anionic molecules) have extraordinarily high affinity and specificity for histones, making them the preferred reagent for binding and neutralizing circulating histones. R A aptamers are designed to selectively target and neutralize circulating hisiones. RNA aptamers are single-stranded oligonucleotides whose binding properties depend on their sequence and structure. Aptamers have significant advantages over other biologies, including stability at room temperature, resistance to serum degradation, and minimal iirrmunogenicity. By using chemically modified RNA, the aptamers are protected from serum degradation and immunogenicity.
Furthermore, alternative approaches to inhibit historic-mediated cellular injury have major limitations. First, while TL s clearly mediate the immune response induced by hi stones, they have no role in hi stone dependent, calcium-mediated cytotoxicity, negating the use of TLR neutralizing antibodies. Second, several other biologies that demonstrate efficacy in animal models have failed to provide a therapeutic benefit in clinical trials or have increased risk of bleeding or toxicity, in addition, many biologies require special handling and storage, special dosing considerations, and risk allergic reactions (e.g, recombinant proteins and antibodies). The development, of RN A aptamer inhibitors of histone-mediated injury is a unique clinical opportunity to interrupt a self-propagating cascade responsible for si gni f ΐ cant morbid ity and mortal it .
MODS is described as ^the development of potentially reversible physiologic derangement involving two or more organ systems not involved in the disorder that resulted in 1CU admission, and arising in th wake of a potentially life-threatening physiologic insult''. (Holzheimer RG, Mannick JA, Marshall JC. The multiple organ dysfunction syndrome. 2001) With the lungs being the organ most commonly involved, acute lung injur (ALT) and acute respiratory distress syndrome (ARDS) may be the primary and most clinically relevant presentation. The annual incidence of ALi/ARDS approximates 200,000 cases in the United States, with mortality approaching 40%. (Bosmann . Grailer JJ, Ruemmler R, Russkamp NF, Zetoune FS, Sarma ,FV, Standiford TJ, Ward PA. Extracellular histones are essential effectors of c5ar-and c-512-mediated. tissue damage and inflammation in acute lung injury. The FASEB Journal. 2013;27:5010-5021). Despite extensive research into the pathogenesis of Aid and clinical trials testing new therapeutics, the improvement in outcomes following ARDS over the pas decade are due to improved strategies of mechanical ventilation and advanced support of other tailing organs, as there remains no effective pharmacotherapy to treat patients with this syndrome. Furthermore, patients who survive frequently have significant psychological and physical morbidity, residual physical limitations and poor quality of life. The development of ALI/ARDS occurs as a consequence of critical illness of diverse etiologies including initial major trauma, toxic inhalation, burns, near-drowning, radiation, sepsis, and blood transfusion. Furthermore, another common manifestation of MODS is haemostatic abnormalities ranging from subclinical activation of blood coagulation (hypercoagulability} to ae'ute disseminated intravascular coagulation (DIC) and subsequent consumption of platelets and coagulation proteins causing bleeding.
Recent evidence suggests that there may be a shared molecular mechanism
responsible tor th self-propagating tissue injur)' associated with these diverse conditions when they progress to MODS, and that involves extracellular hi stones. Eukaryotie genomes are organized in chromatin;, a DNA-protein complex whose basic repeating unit is the nucleosome. The nucleosome consists of DNA wrapped around an oc tamer of histone proteins (two each of hi stones H2A, H2B, 1 13. and H4). (Ailam R, Kumar SV, Darisipudi MN, Anders H-J. Extracellular histones in tissue injury and inflammation. Journal of Molecular Medicine. 2014;92:465-472). However, severely injured tissues release large quantities of niicleosomes into the circulation, which are further broken down into individual histones. Elevated levels of circulating histones can be derived either from damaged and apoptotic cells or from the degradation of neutrophil extracellular traps (NETs), which are structures of extracellular histones and DNA that ensnare and kill bacteria. (Thomas GM, Carbo C, Curtis BR, Martinod K, Mazo IB, Schatzberg D, Cifuni SM, Fuchs TA, von Andrian UH, Hartwig JH. Extracellular DNA traps are associated with the pathogenesis of trail in humans and mice. Blood. 2012;! 19:6335-6343). In critically ill patients, an increase in histone levels within 6 hours of admission predicts mortality. Similarly, in patients with major trauma, circulating histones levels correlate with injury severity, are elevated by 4 hours and peak at about 24 hours after trauma and are still detectable at 72 hours. (Abrams ST, Zhang N, Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi , Kipar A, Yu W.
Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; 187: 160-169).
The present invention provides a therapeutic to rapidly neutralize circulating histones and terminate the self -propagating cycle of tissue injur}1 and development of MODS/ARDS. As indicated by its function in the nucleosome, the ideal reagent. to bind histones (highly cationic proteins) is an oligonucleotide (anionic DNA or RNA molecule). An innovative technology is used to identify short oligonucleotides (RNA ap tamers) that selectively bind to histones known to cause MODS/ARDS. Since histones are highly conserved across species from yeast to humans, the bio-reagents developed and validated are quickly advanced for testing in preclinical models and human clinical trials.
Several lines of evidence have substantiated that circulating histones, not
nucleosomes or DNA, mediate these toxic effects. For example, nucleosorn.es which have been briefly sonicated or exposed to serum (i.e., DNA is degraded and individual hi stones are released) induce endothelial cell death. (Allam R, Kumar SV, Darisipudi MN, Anders H-J. Extracellular histones in tissue injury and inflammation. Journal of Molecular Medic ne. 2014;92:465-472). Human patient serum with histone levels in excess of 50 ,ug/ml is toxic to cultured endothelial cells. (Abrams ST, Zhang N, Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K, Kipar A, Yu W. Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; 187: 160-169). Intravenous injection of recombinant histones in mice causes endothelial damage, cytokine elevation, platelet aggregation, and microvascular necrosis in. the lungs, leading to death. (Abrams ST, Zhang N, Manson. J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K, Kipar A. Yu W. Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; ! 87: 1 0- 169; Xu J, Zhang X, Pe!ayo R, Monestier M, Ammo 11 o CT, Semeraro F, Taylor FB, Esmon NL. Lupu F, Esmon CT.
Extracellular histories are major mediators of death in sepsis. Nature medicine.
2009; 15: 1318-1321). Extracellular histones mediate tissue damage through multiple mechanisms including: (1) disruption of cell membranes, resulting in Ca2< influx and subsequent elevations in intracellular Ca~+ concentrations and cell damage; (2) activation of TLR2 and TLR4, thereby inducing NF-κΒ and pro-inflammatory cytokine production; (3) induction of a pro-coagulant phenotype in platelets, thereby accelerating blood coagulation and enhancing thrombin generation in a mechanism that involves Toll like receptors (TLR) 2 and 4 (Semeraro F, Ammollo CT, Morrissey J{ I, Dale GL, Friese P, Esmon NL, Esmon CT. Extracellular histones promote thrombin generation through platelet-dependent mechanisms: Involvement of platelet tlr2 and tlr4. Blood. 2011 ;1 18:1952-1 61 ); (4) increased gut permeability, which allows endotoxin or bacteria to enter the circulation and prime neutrophils which, in turn, release toxic mediators (i.e., cytokines) to damage lungs and other organs.
Current attempts to abrogate histone-induced injury have limitations. Histones represent one of many damage-associated molecular pattern molecules that can initiate and perpetuate immune response; their potential to amplify tissue injuiy by kill ing other eel is, in addition to their agonistic activity on TLRs, provides a rationale to target histones for therapy. Unfortunately, approaches currently being pursued in experimental models have marked limitations. First, while TLRs clearly mediate the immune response induced by histories, they have no role in calcium-mediated cytotoxicity, negating the use of TLR2/4 neutralizing antibodies. Second, several other biologies that demonstrated efficacy in animai models have failed to provide a therapeutic benefit in clinical trials (activated protein C) or have increased risk of bleeding (heparin, APC) or toxicity (historic deacetylase inhibitors). In addition, many biologies require special handling and storage, special dosing considerations, and risk allergic reactions (e.g., recombinant proteins and antibodies). Given the limitations of current approaches, the development of selective inhibitors of historic-mediated injury is a unique clinical opportunity to interrupt a pathophysiologic cascade responsible for significant morbidity and mortality.
The ideal binding partner of a hi stone (cationic protein) is an oligonucleotide (anionic molecule). Histones normally associate with DNA in the nucleosome. Given their strong negative charge, oligonucleotides (e.g. RNA aptamers) have extraordinarily high affinity and specificity for positively charged histones, making them the preferred reagent for binding and neutralizing circulating histones. No one has previously attempted to use oligonucleotides to inhibit extracellular histones. RN A aptaniers are single-stranded nucleic acids whose binding properties depend on their sequence and structure.
Development and in vitro characterization of RNA aptamers that selectively bind to human histones. SELEX is used to identify RNA aptamers with high affinity to human 3-13 and 114. Studies characterize individual aptamer sequences and define their binding affinity and specificity. (Tuerk C, Gold L. Systematic evolution of ligands b exponential enrichment: RNA ligands to bacteriophage t4 DNA polymerase. Science. 1990;249:505-510). Studies also use cultured human pulmonary endothelial cells, alveolar epithelial cells, and platelets to confirm the efficacy of RNA aptamers at neutralizing histone-rnediated cellular effects.
A. Development of RNA aptamers to human histones 113 and 114. These studies identify RNA aptamers that selectively bind with high affinity to both human histones H3/H4. Briefly, RN A aptamers with specificity for human H3 and 114 were enriched by pre- clearing a chemically modified RNA aptamer library against control proteins (BSA and human IgG) abundant in serum. Those NAs that did not bind serum proteins were collected and incubated with either human H3 or H4. Histone-bound RNAs were collected and processed for the next round of selection. Several rounds of selection were performed to enrich for high affinity binders. High affinity aptamers were enriched by modifying the selection conditions at each round of selection (e.g. increasing the RNArhiston ratio during the positive selection step and reducing the incubation time of aptamers with histones it: has been confirmed that the starting, unenriehed RNA a tamer library (round 0) binds to the historic proteins with low nM affinity (Figure 3A). Following seven rounds of selection RNA sequences were enriched with overall higher affinity (high pM range) for the intended histone target (Figure 3B) (data shown for H3 selection). Finally, round 7 from each selection did not bind to proteins normally found in serum (dat not shown), confirming the specificity of the selected RNAs. After 8-10 rounds of selection, final post-selection, enriched aptamer pool has affinities in the low pM range. The progress of the selections are assessed by determining the sequence diversity of the random regions from round to round by high- throughput sequencing (HTS) and identifying sequence and structure families using bioinformatics analyses. Progress of the selections is assessed by carrying out standard in vitro binding assays with the pools of RNA from each round.
B. In vitro characterization of individual RNA aptamers. individual RNA aptamers identified from the sequence analysis are screened for (1 ) specificity of binding and for (2) ability to inhibit histone activity, fa vitro filter binding assays are performed with the top 10-15 sequences from the HI'S and bioinformatics analysis as previously described. (Thiel KW, Hernandez Lh Dassie IP, Thiol WH, Liu X, Stockdaie KR, Rothman AM, Hernandez FJ, McNamara JO, 2nd, Giangrande PH. Delivery of chemo-sensitizing siRNAs to ber2+-hreast cancer cells using RNA aptamers. Nucleic Acids Res. 20 2;40:6319-6337; Thiel WH, Bair T, Peek AS, Liu X, Dassie J, Stockdaie KR, Behike MA, Miller FJ, Jr., Giangrande PH. Rapid identification of cell- pecific, internalizing RNA aptamers with bioinformatics analyses of a celt-based aptamer selection, PLoS One. 20 2:7:e43836: Thiel WH, Bair T, Wyatt Thiel K, Dassie JP, Roc key WM, Howell CA, Liu ΧΎ, Dupu AJ, Huang L, Owczarzy R, Behike MA, McNamara JO, Giangrande PH. Nucleotide bias observed with a short SELEX RNA aptamer library. Nucleic Acid Ther, 201 i;21:253-2.63). A more extensive quantitative determination of binding affinities via surface plasmon resonance (SPR) is performed for the 5 aptamers that demonstrate the greatest affinity and specific binding in the filter binding assays (Thiel KW, Hernandez LL Dassie JP, Thiel WH, Liu X, Stockdaie KR, Rothman AM, Hernandez FJ, McNamara JO, 2nd, Giangrande PH. Delivery of chemo-sensitizing siRNAs to her2+-breast cancer cells using R A aptamers. Nucleic Acids Res. 2012;40:6319-6337; Huang YZ, Hernandez FJ, Gu B, Stockdaie KR, Nanapaneni K, Scheetz TE, Behike MA. Peek AS. Bair T, Giangrande PH, McNamara JO, 2nd. RNA aptamer-based functional ligands of the neurotrophin receptor, trkb. Mol Pharmacol .
2012;82:623-635; Hernandez LL Flenker KS, Hernandez FJ, Klingelhutz. AJ, McNamara JO, 2nd, Giangrande PH. Methods for evaluating cell-specific, cell-internalizing RNA aptamers. Pharmaceuticals (Basel). 2013;6:295-319). The best two candidate aptamers that selectively bind H3/H4 in the low pM-low nM range are used to test efficacy in cells in culture. Mutated aptamers that have weak-to-no affinity for histones serve as negative controls. Cells also receive aptamers without histones as a control. To model circulating histone content, human pulmonary microvascular endothelial cells (EC), human alveolar epithelial cells, or human platelets are exposed to histories purified from calf thymus'" (contains a heterogeneous mixture of core histones) (Abrams ST, Zhang N , Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi Kt Kipar A, Yu W. Circulating histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013:187: 1 0-169;
Semeraro F, Ammollo CT, Morrissey JH, Dale GL, Friese P, Esmon NL, Esmon CT.
Extracellular histones promote thrombin generation through platelet-dependent mechanisms: Involvement of platelet t!r2 and tlr4. Blood. 201 1 ;1 18: 1952-1961 ) in the presence or absence of aptamer. Human patient serum with histone levels in excess of 50 pg/ml is toxic to EC (Abrams ST, Zhang N, Manson J, Liu T, Dart C, Baluwa F, Wang SS, Brohi K, Kipar A, Yu W. Circulatin histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013;187: 160-169; Xu J, Zhang X, Pelayo R, Monestier M, Ammollo CT, Semeraro F, Taylor FB, Esmon NL, Lupu F, Esmon CT.
Extracellular histories are major mediators of death in sepsis. Nature medicine.
2009:15: 1318-1321 )); therefore this concentration is used in vitro. Dose response curves tor the aptamers are determined in. vitro and will define the concentration used in in. vivo studies. Studies also explore timing of aptamer delivery, in particular if treatment after exposure to circulating histones can prevent cellular toxicity. Cellular toxicity is determined by performing the following measurements: (1) Measurement of calcium influx: Intracellular calcium concentration will be measured using established protocols with fura 2-AM as the fluorescent probe. (2) Measurement of TLR activation: Expression of IFN-γ, IL-Ιβ, 1L-6, and TNF-a will be assessed by PCR. (3) Detection of cell toxicity: Live, apoptotic, and dead cells are determined by flow cytometry following staining with annexin-V and propidium iodide (PI). (4) Measurement of tramcellular resistance : Transendothelial. and transepithelial electrical resistance is measured across a confluent monolayer using an electrical eell- substrate impedance sensing system.
Platelet aggregation : The release of histones from dying cells is associated with microvascular thrombosis. Histone H3 and 114 have been found to be responsible for directly inducing aggregation of human platelets. As an independent measure of aptamer inhibition of histone activity, the effect of individual, histone aptamers is evaluate on hi stone-mediate platelet aggregation. (Xu J, Zhang X, Pelayo , Monestier M, Ammollo CT. Scmeraro F, Taylor FB, Esmon NL, Lupu F, Esmon CT. Extracellular histones are major mediators of death in sepsis. Nature medicine. 2009; 15:1318-1321). it has been shown that histones H3/H4 induce pronounced platelet aggregation which can be inhibited with addition of RNA aptamers (Fig, 4A). importantly, aptamer inhibition of histone-mediated aggregation was more pronounced with selected aptamer pools (R5 pool for H3 ; R.3 pool for 114) compared to the unselected RNA poo! (R0). As a measure of specificity, the RNA aptamers did not reverse collagen-mediated platelet aggregation (Fig. 4B). For these studies, blood is collected from human volunteers, platelet-rich plasma prepared, and platelets isolated. Using the historic and aptamer treatment protocol described above, studies are performed in a collagen- coated flow chamber perfused with washed human platelets as previously described. (Xu J, Zhang X, Pelayo R, Monestier M, Ammoilo CT, Semeraro F, Taylor FB, Esmon NL, Lupu F, Esmon CT. Extracellular histones are major mediators of death in sepsis. Nature medicine. 2009;15:1318-1321 ). In separate studies, platelet surface marker expression (activated «2bP3 and P-selectin) are detected by fluorescent antibodies followed by flow cytometry.
Evaluate efficacy and safety of histone-speeifie RNA aptamers in vivo. The efficacy of the histone speci fic aptamers in mouse models of MODS/ARDS, TRALI, and smoke inhalation is evaluated. Safety is evaluated in immune -competent mice and human peripheral blood mononuclear cells (PBMCs). Finally, efficacy in humans is evaluated by obtaining serum from critically ill patients and test the ability of aptamers to neutralize histone-mediated toxicity.
A. Animal models of disease: These studies test the efficiency of the histone RNA aptamers in three independent mouse models of histone-induced lung injury ( MODS/ARDS, TRALI, inhalation lung injury). (Abrams ST, Zhang N. Manson J, Liu T, Dart C, Baiuwa F, Wang SS, Brohi , BCipar A, Yu W. Circulatin histones are mediators of trauma-associated lung injury. American journal of respiratory and critical care medicine. 2013; 187: 160- 169; Ward PA, Grailer JJ. Lung inflammation and damage induced by extracellular histones.
Inflammation and Cell Signaling. 2014:1 ; Chen R, ang R, Fan XG, Tang D. Release and activity of histone in diseases. Cell Death Dis. 2014;5:el 370). For each protocol, a control aptamer (mutant aptamer that has low-to-no affinity for histones) is administered and the H3 and H4-specific aptamers (one for each histone) is identified with the in vitro functional experiments as most effective at neutralizing histone-mediated toxicity. Aptamers are tested at different doses and administered at various times after the '"injury". Animals are pretreated with the aptamers (0.1 mg kg or 1 mg/kg, IV) at various times related to the injury to test the ability lo prevent development or abort progression of tissue injury. The doses are adjusted as indicated based on the binding data to identity the lowest effective dose. In separate experiments, the aptamer is administered at various times after delivery of the histones (5, 15, and 30 minutes) to examine the ability of the aptamer to prevent injury after the presence of circulating histones. Nonin vasive determination of respiratory function (reduced 02 saturation) is continuousl monitored using a pulse oximeter. After 3h (or at the time of death if occurs prior to 3b) the mice are sacrificed and the following performed:^ Alveolar permeability and inflammation: Tracheas are isolated and canriulated and the lungs flushed with sterile PBS. Cytospins of BALF cells are stained with modified Wright-Giemsa.
Alveolar permeability is assessed by quantitative detection of mouse albumin in BALF by ELISA. (2) Lung histology. Lungs are fixed, embedded and sectioned. Sections are stained with hematoxylin and eosin (H&E) and Mallory's phosphotungstic acid -hematoxylin staining for fibrin then examined by a pathologist for evidence of neutrophils: in alveolar spaces, capillary congestion, infra-alveolar hemorrhage, fibrin deposits and thrombi. (3) TLR
activation. Blood is collected and serum isolated and stored at -80°C. Cytokines/chemokines are all quantified using a customized multiplex magnetic bead panel kit. (4) Platelet FACS. Platelet surface marker expression (activated u2b|¾3 and P-seleeiin) as a measure of platelet activation, are detected by fluorescent antibodies followed by flow cytometry.
N ' onspecific immune activation: Thorough studies are performed to evaluate any potential nonspecific immune effect of the hi stone RNA aptamers. For these studies, doses of the RNAs that are 10-fold greater than those found to be effective in vivo, are injected in the tail veins of C57/BL6 male mice. Blood from treated animals is collected by cardiac puncture at I h, 6h and 12h after the first administration of the RNA. Collected blood sera is analyzed using the Bio-Rad Biople platform to assess the levels of 23 different cytokines ( aul R, Johnson , Scholz H. Man' G. Performance of the bioplex 2200 autoimmune vasculitis kit. Aiitoimmun Rev. 2009;8:224-227).. Spleens and livers from treated mice will also are collected at early (O.Shrs), intermediate (6hrs), and late (24brs) time points following injection of the RNA and processed for total RNA. Levels of inflammatory cytokines, interferons and viral RNA recognition genes are quantified using RT-qPCR as previously described. (Dassie JP. Hernandez LI, Thomas GS, Long ME. Rockey WM, Howell CA, Chen Y, Hernandez FJ, Liu XY, Wilson ME. Targeted inhibition of prostate cancer metastases with an RNA aptamer to prostate-specific membrane antigen. Molecular Therapy. 2014).
Polyinosmicrpolycytidylic acid (Poly I:C) is used as a positive control for immune
stimulation. Histological analysis of tissue sections is performed to determine if specific 'immune cell populations infiltrate specific tissues in response to RNA therapy. (Judge AD, Bola G Lee AC, MacLachlan 1. Design of non-inflammatory synthetic siRNA mediating potent gene silencing in vivo. Mol liter. 2006;13:494-505).
C Obtain serum from critically ill patients and test ability of aptamers to neutralize hhtone-mediated toxicity. Blood samples are collected via initial placement of a 16g or larger peripheral JV into citrated tubes after admission to the intensive care unit. Following centrifugation, control or histone targeting aptamers are added to the platelet-rich-plasma. Platelets are isolated and surface marker expression (activated ( 2bP'3 and P-selectin) is detected b fluorescent antibodies followed by flow cytometry. The serum is added to cultured microvascular endothelial cells and toxicity measured as described above.
Circulating histone levels are measured in the serum using an. ELISA assay.
EXAMPLE 2
Schematic of in vitro SELE technology. The DNA aptamer librar contains a variable region of 20 base flanked by two constant regions (Figure 5): 1) The DNA template library is in vitro transcribed into RNA using a modified T7 RNA polymerase enzyme that enables the incorporation of chemically modified NTPs. 2) The RNA library is incubated with an irrelevant protein (control protein) for the preelear step to remove non-specific binders. 3) Those RNA aptamers that do not bind the control protein are then incubated with a target protein. 4) Unbound aptamers are removed and RNA aptamers bound to target protein are recovered, reversed transcribed using RT-PCR, 5) PGR amplified, and 6) in vitro transcribed in the presence of chemically modified NTPs (e.g. 2,-fluoro modified
pyrimidines). Several rounds (2-10) are carried ou to generate a library of aptamers with high affinity and specificity for the specific target.
Aptamer inhibitors of extracellular histories for the treatment of sepsis. Tissue damage and diseases cause cells to. release histones into the circulation (referred to as circulating or extracellular histones) (Figure 6), The circulating histones go on to propagate release of more histones by the tissue injury and cell death.
Complexity assay (Figure 7). A complexity assay was performed by mixing 10 μ! of 0.5μ DNA duplex library from selected rounds to 10 μΐ of SYBR green (BioRad. 170- 8882). These samples were run in a 96 well plate on a real-time PC machine (Eppendorf. Mastercycler epgradient S with realplex2) using the gradient PGR protocol, 95°C for 1.5 min; 95°C-25°C reducing the temperature every 15 sec; 4°C hold). The florescence from the raw data (performed in triplicate) was averaged then plotted as fluorescence intensity (SYBR) vs. temperature (C°). Reduction in complexity was shown by the relative shift of the DNA melt curve towards higher temperatures, DNA from Round 0 and H4 selected rounds 2 and 6 went through a reverse melt curve. Higher library complexity was seen in rounds 0 and 2 by a relaiive shift of the DNA raelt curve towards lower temperatures, whereas. Round 6 shifted to a higher temperature indicating a lower library complexity.
Sequence enrichment of aptamers for istones (Figure 8). The sequence enrichment (% Enrichment) was determined for aptamers from the A) 113.2 and B) H4 aptamer selections. Sequence enrichment, was determined for each round of selection
(Selection Round) from read counts determined fro Illumina sequencing using the following equation; % Enrichment- 1 -(unique sequences/total sequences). Unique sequences refers to the number of different sequences identified within a selection round and total sequences refers to the total number of reads from a selection round including all duplicate reads.
Separation of non-selected aptamer sequences from selected aptamer sequences (Figures A-9B). The A) read counts (Cluster Size) and S) round representation of H3.2 (left panels) and 114 (right panels) selection rounds (1-8) were compared to a pool of non- selected aptamers (round 0) to identify selected aptamers. Selected aptaniers exhibit two properties, abundance and persistence. Selected aptamer will accumulate during the selection process (abundance) and the selected aptamers will appear in more than one selection rounds (persistence). The read count (Cluster Size) analysis examines aptamer abundance by plotting the distribution of read counts within each selection round of aptamer (rounds 0-8). The round representation analysis examines aptamer persistence by first determining the number of selection rounds each aptamer sequence was observed and second plotting the distribution of round representation for al l aptamers within each selection round. Aptamers with read counts significantly greater than the read counts observed in round 0 (>4 reads) and that appear in at least two selection rounds are classified as selected.
Histone aptamer sequences (Figure 10), These aptamers have read counts significantly greater than the read counts observed in round 0 (>4 reads) and that appear in at least two selection rounds are classified as selected. These nine aptamers had the largest Log2 fold change between rounds 6:8. They were further selected by their presence in either both or one of the selections. The full list of selected aptamers is in Table 1 below.
Predicted secondary structure of selected aptamer sequences (Figure 11). The sequences from. Figure 10 were imputed into the RNA fold program to generate the possible secondary structures ( Website: http://rna.tbi '.uni ie.ac.af'cgi-bin/RNAi ld.cgi) Filter Binding Assay Protocol (Figure 12). One hundred pinoles of purified A aptamers were dephosphorylated by Bacterial Alkaline Phosphatase (Promega) and were labeled, with 32P-gamma ATP (6,000 Ci/mmo.1) by T4 PNK (Promega). The radiolabeled aptamers were then purified on (325 columns (GE Healthcare) and counted on a Scintillation Counter. The RNA amount was adjusted to 2,000 CPM/μϊ and folded by heating at 95 °C for
5 minutes, 65 °C for 5 minutes, and 37 °C for 10 minutes in the selected buffer. Serial dilutions of Histones starting from 250nM were made in selection buffer. Five microliter of
RNA was incubated with 15 μΐ of protein for 5 minutes at 37 °C filtered through
Nitrocellulose (top) and Nylon (bottom) double-filters using dot-blot apparatus (Na!gene, Rochester, NY). CL-X film was exposed by membranes for 30 minutes at RT and was imaged on the typhoon. ImageJ 1.38* software (Wayne Rasband; N3H, Bethesda, MD) was used to determine relative optical density of radiographed spots in NC-filter binding assay, these data been used to calculate amount of radioactive RNA in each spot. (Wong i, Lohman TM. A double-filter method for nitrocellulose-filter binding: application to protein-nucleic acid interactions. Proc Natl Acad Sci USA 1993; 90: 5428-32.)
Progression of RNA aptamer selection for histones H3 and H4 measured by filter binding assay ( Figures A-13 B). Bind ing of hi stone H3.2 (a) and histone H4 (B) to uiiseSected RN A aptamer library'' (RDO) and selected RNA aptamer pool from round 8 of selection (RD8). A leftward shift in binding is indicative of enrichment for higher affinity aptamers. 113 RD8 KD=.6nM; H4 Rd8 - KD.3nM
Binding data of rounds 0 and 8 of selection to calf thymus histones (Figures 14A- 1 B). Binding of selected library (round 8 - Rd8) and un selected library (RDO) to calf thymus histones was measured by filter binding assay as above. Rd8™ squares; RDO = circles. Histone (calf thymus) is a natural mixture of the histones HI , H2A, Ή2Β, H3 and H4. These histones still retain their posttranslational modifications.
Binding specificity of RNA aptamers (Figures 15A-15B2). Binding of selected library (round 8 - Rd8) and control RNAs (NSC and A9g) to histones H3 and H4 was measured by filter binding assay as above. Rd8 = triangles; NSC = squares; A9g = circles.
Binding of individual RNA aptamers to histone H3 and H4 proteins (Figures 16A-16I). Binding of aptamers KU1 (A), KU2 (B), KU 3 (C), KU4 (D), U5 (E), KU6 (F), U 7 (G), KU 8 (H), KU 9 (1) to histones 113 (light circles) and H4 (dark squares) measured by filter binding assay.
Binding affinities ( j ) of the different clones in Figures 16A-16I for H4 and 143 (Figure 17). Binding of individual RNA aptamers to histone HI, H3, and H4 proteins (Figures 18A-18I). Binding of aptamers KU 1 (A), KU2 (B), KU 3 (C), U4 (D). KU5 (E), KU6 (F), KU 7 (G), KU 8 (H), KU 9 (I) to histones Hi (triangles), H3 (light circles), and H4 (dark squares) measured by filler binding assay.
Binding affinities (Kj ) of the different clones in figure 4 for H I , B3 and 114
(Figure 19).
Binding of individual RNA aptamers to histone H3, H4, and Human Serum (Figures 20.4-20! ). Binding of aptamers KU1 (A), KU2 (B), KU 3 (C), KU4 (D), KU5 (E), KU6 (F), KU 7 (G), KU 8 (H), KU 9 (1) to Human Serum (triangles), H3 (light circles), and H4 (dark squares) measured by filter binding assay.
Binding affinities (K|>) of the different clones in figure 5 for Histories 113, H4, and Human Serum (Figure 21 ).
Binding of individual RNA aptamers to histone J 13, 114, and Human Albumin (Figures 22A~22i). Binding of aptamers K I (A), KU2 (B), KU 3 (C), KU4 (D), KU5 (E), KU6 ( F). KU 7 (G), KU 8 (H), KU 9 (1) to Human Albumin (triangles), H3 (light circles), and H4 (dark squares) measured by filter binding assay,
Binding affinities ( Kn) of the different clones in figure 6 for Histones H3, H4, and Human Albumin (Figure 23).
Binding of individual RNA aptamers to histone HI , H2A, H2B, H3, and 114 proteins (Figures 24A-24C). Binding of aptamer clones (A) KU5, (B) KU7. (C) KU9 to histones HI (circles), H2A (squares), H2B (upward triangle), H3 (downward triangle), and H4 (diamonds) measured by filter binding assay.
Binding affinities (¾) of the different clones in figure 7 for histones II I , H2A, H2B, H3, and H4 (Figure 25).
Schematic of platelet aggregonieter (Figure 26). Platelet poor plasma [PPP j is stirred in a cuvett at 37°C and the cuvette sits between a light course and a photocell. When an agonist is added the platelets aggregate and absorb less light and so the transmission increases and this is detected by the photocell. Schematic adapted from http://pract.ical· haemostasis.com/Platelets/platelet function testing StaJittnl
RNA aptamers inhibit histone-mediated platelet aggregation (Figures 27A-27D).
Platelet aggregation was performed with washed human platelets and quantitated using an aggregometer at 2 min intervals. (A) Histones 113 and H4 induce platelet aggregation.
Addition of the un selected round 0 aptamer pool (10 nM) reduces platelet aggregation. A more pronounced inhibition of platelet aggregation is observed with selected RNA pools (Rnd5 for H3 and Rnd3 for H4). (B) RNA aplanicrs have no effect on collagen-mediated platelet aggregation. Heparin (I IJ/mL) reverses hi stone- mediated platelet aggregation. (C)
Selected aptamer KU7 high dose ( U7HD, 100'OnM) showed pronounced inhibition of histone H4, while KU7 low dose ( U7LD, !OOn ) inhibited only slightly. (D) RNA apiamcr U7 only did not induce platelet aggregation.
Cytotoxicity of hislones to endothelial cells (Figure 28). EA.hy926 cells were cultured with human ( A) histone 113, (B) histone H4, or (C) aptamers (KU , KU4, or NSC) for 1 hr at 37°C. Cell damage was measured by flow cytometry for propidium iodide staining.
Histone concentration: 0, 12.5, 25, SO, 1.00 μ.«/ηιί and aptamer concentration: 0.0, 12.5, 25, 50, 100 nM, NSC refers to a non-specific control aptamer sequence that does not bind histones.
Cytotoxicity of histones to epithelial cells (Figure 29). Epithelial cells (A549) were incubated With 114 (20ug/mi) or the aptamers KU5, U6, KU7, U9 or control aptamer NSC (all aptamers at SOOuM) concentration for 1 hour. After the hour the cells were treated with propidium iodide and flow cytometry was performed. The percent of all cells that did not stain for PI arc shown.
Cytotoxicity of calf thymus histones to epithelial cells (Figure 30). Epithelial cells (A549) were incubated with calf thymus histones (25, 5 , 100 pg/ml) concentration for 1 hour. After the hour the cells were treated with propidium iodide and flow cytometry was performed. The percent of all cells that did noi stain for PI are shown.
Apoptosis activation of histones to epithelial cells (Figure 31). A549 cells were cultured with human histone H4 for 6 hr at 37°C. Cel l apoptosis was measured by
florescence using the cellevent Caspase 3/7 kit. Histone concentration: 0, 35.6, 47.5, 63.3, 84.4, 1 12.5. 150, 200ug/mL.
Histone induced toxicity in vivo (Figure 32). Noninvasive measurement of respiratory rate, heart rate, and oxygen saturation were continuously monitored in a mouse, which was injected with 1 80mg/ml of calf thymus histones via tail vein. Arrow indicates time of death. Respiratory rate - lowest starting line; % oxygen saturation - highest starting line; heart rate - middle starting line.
Summary
As shown in the data above, aptamers were identified that bind to histones 1:13.2 and H4 using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology (Figure 5). Following eight rounds of selection (R8), RNA sequences were enriched with overall higher affinity (p'M vs. nM range) for the intended histone target compared to the starling, unenriched RNA aptamer library (round 0, R0) (Figure 12). The progression of the H3.2 and 1-14 selections was assessed by determining the sequence diversity of the random regions from round to round by high -throughput sequencing (UTS) and identifying enriched sequence and structure families using bioinformatics analysis (Figures 7-9). individual RNA aptamer candidates (9 total) identified from the
sequence bioinformatics analyses were screened to determine binding affinities and specificities (Figures 15-24). Binding data are shown for the top nine candidate aptamers (most highly enriched aptamers following 8 rounds of selection) identified from the bioinformatics analysis (Figure 10). Although the aptamers were selected against H3.2 and 1 Ϊ4, binding to histones i ll (Figure 17- 18 and Figure 23-24), H2A and H2B (Figures 23- 24) (the equilibrium dissociation constants are reported in the tables) were also determined. The binding data confirm that these aptamers bind with high affinity ( D - pM to low nM range) to histones H3.2 and H4 (Figures 23-24). For example, Aptamer KU5 also binds to histones H2A and H2B (affinities in the low nM range) but not HI . Interestingly, aptamer KU7 binds to HI and ϊ I2B (KD - low nM range) but not H2A and aptamer U9 does not bind to histones H2A or H2B but has high affinity for histone HI ( KD 5.3 nM). In addition, binding specificity for the histone proteins was demonstrated by confirming that the RNAs do not bind to proteins in human serum (Figures 9-20) or to human serum albumin (a protein which is highly abundant in serum) (Figures 21-22). As an additional measure of specificity, it was confirmed that two control aptamers (NSC and A9g) do not bind to the histone proteins (Figure 14),
In vitro functional data:
Platelet aggregation (Figures 25-26); The release of histones from dying cells is associated with microvascular thrombosis. Histone H4 and, to a lesser extent H3.2, have been found to be responsible for directly inducing aggregation of human platelets by driving plasma thrombin generation through the activation of TLR2 and TLR4. It is shown that histones H3.2 and H4 induce pronounced platelet aggregation (plotted as % aggregation), which can be inhibited with addition of heparin (positive control) and the un selected RNA aptamer pool (R0). Importantly, aptamer inhibition of hi stone-mediated platelet aggregation was more pronounced with selected histone aptamers (data shown for U7) compared to the unselected RNA pool (R0). As a measure of specificity, the RNA aptamers did not reverse collagen-mediated aggregation. Aptamer safety profile in vitro (Figures 27-31): Circulating hi stones cause endothelial eel! toxicity, contributing to vascular dysfunction, platelet aggregation and tissue ischemia. It was confirmed that the exposure of cultured epithelial cells with histone H4 (20 ng/ml for 1 hours) results in cell death. In contrast, the candidate histone-selectiye aptaniers ( U5, U7, KU9) did not induce cell toxicity (1 μΜ for 1 hour),
Histone-raediated toxicity in vivo (Figure 32):
Histone administration causes neutrophil migration, endothelial injury and
dysfunction, hemorrhage, and thrombosis, which finally result in animal death at doses as low as 50 mg/kg body weight. It was confirmed that injection of histories into (he circulation leads to organ failure and subsequent death in a mouse model of multiple organ dysfunction syndrome (MODS), in this experiment, a C57/BL6 mouse was injected with 180 xgimL of histone from calf thymus while noninvasively monitoring the animal's respiratory rate, heart. rate and oxygen saturation. Following intravenous histone injection, oxygen saturation gradually declined ove several minutes and the mouse died. These data confirm, the acute lung injury caused by circulating histones and identify a model for proposed studies to evaluate the ability of R A aptamers to protect from histone-fnediated lung injury in vivo.
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TABLE 1
# in H
HTS read variabie region RNA sequences # in H3 selection selection Selected sequenc
TTTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0001. _H32 0001. _H04
TTG G GTGTAGG GTG GTACCG GGGAGGAGGAUGCGGUUGGG UGUAGGGUGGUACCGCAGACGACUCGCCCGA 0002. .H32 0004. .H04 KUl
ATTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0003. „H32 0019_ .H04
G CCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCGU UCUGCCCUCCCCAGACGACUCGCCCGA 0004. .H32 0003. .H04
G CCTTTCGTTCTGAC CTCCC GGGAGGACGAUGCGGGCCU UUCGU UCUGACCUCCCCAGACGACUCGCCCGA 0005. _H32 0011_ .H04
ATTGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACCGCAGACGACUCGCCCGA 0006. _H32 0017_ _H04
CAGAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0007. _H32 0320_ .H04
TGTAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGG UACCGCAGACGACUCGCCCGA 0008. _H32 0090_ .HQ4
TCCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0009. .H32 0002. _H04
TTT6AAGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGGUGGGUCCGCAGACGACUCGCCCGA 0010. ,H32 0007_ _H04
TATTGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 0011. _H3 0030. .H04
TTTGGGGTTTTCCCGTGCCC GGGAGGACGAUGCGGUUUGG6GUUUUCCCG UGCCCCAGACGACUCGCCCGA 0012_ H32 0006_ HQ4
ATAGGGAGAAGGGTGAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGUGAACCGCAGACGACUCGCCCGA 0013. _H32 0769. .H04
TTGGGTGTAGGGTGHACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGUUACCGCAGACGACUCGCCCGA 0014. .H32 0064 .H04
TTGGGTGTAGGGTGGTACTG GGGAG6ACGAUGCGGUUGGGUGUAGCGUGGUACUGCAGACGACUCGCCCGA 0015. H32 0314. _H04
GTCCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGGUCCUG UCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0016. „H32 0010. _H04
TTTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA Q017. _H32 0012. .H04
TTTGAAG G G AG ATGGGTCC6 6GGAGGACGAUGCGGUUUGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 0018. _H32 0013_ .H04
TTTGAAGGGGGAT66GTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0019. .H32 0016. .H04
GTCTTCTG G CTT ATCG TC CC GGGAGGACGAUGCGGGUCU UCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 0020. .H32 0028. _H04
TGACTCGTCTGTTTCGTCCC GGGAGGACGAUGCGGUGACUCGUCUGUU UCGUCCCCAGACGACUCGCCCGA 0021. .H32 0056. .H04
TCCTT6TCGTCTTGCGTCCC GGGAGGACGAUGCGGUCCUUG UCGUCUUGCGUCCCCAGACGACUCGCCCGA 0022_ -H32 0009. .H04
TTTGAAGGGTGATGGGTCCC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 0023. _H32 0008., HOI U2
TTC G AAG G GTG ATG G G TCCG G6GAGGACGAUGCGGUUCGAAGGGUGAUGGGUCCGCAGAC6ACUCGCCCGA 0024. „H32 0021_ H04 KU3
TGTAGGGAGGTAGTTATCGT GGGAGGACGAUGCGGUGUAGGGAGGUAGUUAUCGUCAGACGACUCGCCCGA 0025. .H32 4238_ .H04
GTTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGGUUGAA6GGUGAUGGGUCCGCAGACGACUCGCCCGA 0026. .H32 0015. _H04
TTGAAGGGTGATGGGTCCG GGGAGGAC6AUGCGGUUGAAGGGUGAUGGGUCCGCAGAC6ACUCGCCCGA 0027. .H32 0018. _H04
n in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CTTGTCGTCTTGTTCGTCCC GGGAGGACGAUGCGGCUUGUCGUCUUGUUCGUCCCCAGACGACUCGCCCGA 0028. _H32 0226_ _H04
TTGGGTGTAGGGTGGTGCCG GGGAGGACGAUGCGGUUGG6 UGUAGGGUGGUGCCGCAGACGACUCGCCCGA 0029. „H32 0115. .H0
CTTG AAG G GTG ATG 6GTCCG GGGAGGACGAUGCGGCUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0030. _H32 0029. ,H04
CG TTTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGCGUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 0031. .H32 0053„ ,H04
TTTGAGGG GTG ATG 6 GTCCG GGGAGGACGAUGCGGUUUGAGGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0032. .H32 0031_ .H04
TCTG AAGG GTG ATG G GTCCG GGGAGGACGAUGCGGUCUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0033. _H32 0036_ H04
TGTCGTAGTCTATCCGCCCG GGGAGGACGAUGC6GUGUCGUAGUCUAUCCGCCCGCAGACGACUCGCCCGA 0034. _H32 0070. H04
TTTGAAGGGTAATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUAAUGGGUCCGCAGACGACUCGCCCGA 0035. .H32 00 5_ H04
TTTG A AG G GTG ATG G G ACCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGACCGCAGACGACUCGCCCGA 0036. .H32 0092_ .H04
TTTG AAGGGTGATGGGTCTG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCUGCAGACGACUCGCCCGA 0037. .H32 0058. ,H04
TTTGAAGGGTGATGGGCCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGCCCGCAGACGACUCGCCCGA 0038. _H32 0038. _H04
TTGGGTGTAGGGAGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGAGGUACCGCAGACGACUCGCCCGA 0039. _H32 0147_ .H04
ATTGGTG AAGG GA G GTG CTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUGCUGCAGACGACUCGCCCGA 0040. .H32 1522_ H04
TTTG AAGG ATG ATGG GTCCG GGGAGGACGAUGCGGUUUGAAGGAUGAUGGGUCCGCAGACGACUCGCCCGA 0041. _H32 0066. .H04
TTTG AAG AGTG ATGG GTCCG GGGAGGACGAUGCGGUUUGAAGAGUGAUGGGUCCGCAGACGACUCGCCCGA 0042. .H32 0061. _H04
TTTG AAGG GTG ATAG GTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUAGGUCCGCAGACGACUCGCCCGA 0043. .H32 0054_ .H04
TCTTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCUUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0044. _H32 0127. .H04
TCCTGTCGTCTGTTCGTCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCCAGACGACUCGCCCGA 0045. .H32 0040. .H04
TTTGAAAGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAAGGUGAUGGGUCCGCAGACGACUCGCCCGA 0046. .H32 0069. .H04
TTGGGTGTAGGGCGGTACCG GGGAGGAC6AUGCGGUU66GUGUAGGGCGGUACCGCAGACGACUCGCCCGA 0047. H32 0133. .H04
TTTG AAG GGT6ATG GATCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGAUCCGCAGACGACUCGCCCGA 0048. _H32 0075. _H04
TTTTGAAGG GTG ATGG GTCCG GGGAGGACGAUGCGGUUUUGAAGGGUGAUGGG UCCGCAGACGACUCGCCCGA 0049. _H32 0042_ .H04
TTTGAAGGGTGACGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGUGACGGGUCCGCAGACGACiJCGCCCGA 0050. .H32 0041. .H04
TCCTGTCGTCTTTGCGTCCC GGGAGGACGAU6CGGUCCUGUCGUCUUUGCGUCCCCAGACGACUCGCCCGA 0051. _H32 0014. .H04
ATTGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGAUUGGUGUAGGG UGGUACCGCAGAC6ACUCGCCCGA 0052. _H32 2494. .H04
CTATTGGTGAAGGGTGGTG GGGAGGACGAUGCGGCUAUUGGUGAAGGGUGGUGCAGACGACUCGCCCGA 0053. .H32 2270. _H04
ACGTGGTGTAGGG6GGTGTC GGGAGGACGAUGCGGACGUGGUG UAGGGGGGUGUCCAGACGACUCGCCCGA 0054. _H32 3210. _H04
TTTGAAGGGTGTTGG GTCCG GGGAGGACGAUGCGGU UUGAAGGGUGU UGGGUCCGCAGACGACUCGCCCGA 0055 H32 0026 H04
# in H4
HTS read variable region R A sequences ft in H3 selection selection Selected sequenc TTTGAAGGGTGATGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGUCCGCAGACGACUCGCCCGA 0056. „H32 0082_ H04
TTTGAATGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAUGG UGAUGGGUCCGCAGACGACUCGCCCGA 0057, _H32 0059, .H04
GTTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGGUUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA DQ58. H32 2207_ .H04
TTTG AAG G GTG ATGTGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGUGUCCGCAGACGACUCGCCCGA 0059. _H32 0052. H04
ATTACGAG CTTGTGCCTCG GGGAGGACGAUGCGGAUUACGAGCUUGU UGCCUCGCAGACGACUCGCCCGA 0060. _H32 0297_ H04
TTTG G AG GGTG ATG G GTCCG GGGAGGACGAUGCGGUUUGGAG6GUGAUGGG UCCGCAGACGACUGGCCCGA 0061. .H32 0Q62_ .H04
TTTG AAG G GTG ATTG GTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUUGGUCCGCAGACCACUCGCCCGA 0052. .H32 0071, .H04
TTGGGTGTAGGGGGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGGUACCGCAGACGACUCGCCCGA 0063, _H32 0194. H04
TTTG AAG G GTG ATG AGTCCG GGG AGGACG AUGCGG UUUGAAGGGUGAUGAGUCCGCAGACGACUCGCCCGA 0064, .H32 0104_ .H04
TTTG A AGG GTG ATGG GTTCG GGG AGGACG AUGCGG UUUGAAGGGUGAUGGGUUCGCAGACG AC UCGCCCGA 0065. .H32 0117_ .H04
TTTG A AGG GTG ATGGGTCCA GGG AGGACG AUGCGGUUUGAAGGGUGAUGGGUCCACAGACGACUCGCCCGA 0066. .H32 0072. H04
CGATTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGCGAU UCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 0067. .H32 0023_ H04
TTTG GTG AAGGG AGGTACTG GGG AGGACG AUGCGG UUUGGUGAAGGG AGG UACUGCAGACGACUCGCCCG A 0068. _H32 3040. H04
CGACTCGATCTGTTCGTCCC GGGAGGACGAUGCGGCGACUCGAUCUGUUCGUCCCCAGACGACUCGCCCGA 0069. „H32 0051. H04
ATTGGTGAAGG GAGGTTGC GGGAGGACGAUGCGGAUUGGUGAAGGGAGG UUGCCAGACGACUCGCCCGA 0070. .H32
TTTG A AGG GTG ATGG CTCC6 GGG AGGACG AUGCGGUUUGAAGGGUGAUGGCUCCGCAGACGACUCGCCCGA 0071. H32 0176_ _H04
TC CTG G CTTGTCG TTGTCC C GGGAGGACGAUGCGGUCCU6GCUUGUCGUUGUCCCCAGACGACUCGCCCGA 0072. H32 D043_ _H04
TTTGAAGGGTGATGGGTCG GGG AGGACG AUGCGGUUUGAAGGGUGAUGGGUCGCAGACGACUCGCCCGA 0073. _H32 0186_ .H04
GCCTTTCGTTCTGCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UCUGCCUCCCCAGACGACUCGCCCGA 0074. .H32 0033_ _H04
TTCTGGCTTGTCGTTGTCCC G6GAGGACGAUGCGGUUCUGGCUUGUCGUUGUCCCCAGAC6ACUCGCCCGA 0075. .H32 0188. _H04
ATTGGCGAAGGGAGGTACTG GGG AGGACG AUGCGGAUUGGC6AAGGGAGGUACUGCAGACGACUCGCCCGA 0076. .H32 3403. „H04
TTTGAAGGGTTATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUUAUGGGUCCGCAGACGACUCGCCCGA 0077. .H32 0091. „H04
TTTGAAGGGTGCTGGGTCCG GGG AGGACG AUGCGG UUUGAAGGGUGCUGGGUCCGCAGACGACUCGCCCGA 0078. .H32 0067. _HG4
TTTG AAG TG TG ATGG G TCCG GGG AGGACG AUGCGGUUUGAAGUGUGAUGGGUCCGCAGACGACUCGCCCG A 0079. .H32 0096. _H04
TTGG G AG TAG G GTG GTACCG GGGAGGACGAUGCGGUUGGGAGUAGGGUGGUACCGCAGACGACUCGCCCGA 0080. _H32 0223. H04
TTTGATGGGTGATGGGTCC6 GGGAGGACGAUGCGGUUUGAUGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0081. _H32 0099. _H04
TTGGGCGTAGG GTG GTACCG GGGAGGACGAUGCGGUUGGGCGUA6GGUGGUACCGCAGACGACUCGCCCGA 0082. ,H 2 0244. _H04
TTTG A AG G G TG ATG G TTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGUUCCGCAGACGACUCGCCCGA 0083 H32 0102 H04
# ίη H4
HTS read variable region RNA sequences # in Hi selection selection Selected sequenc
ATTGGTGAAGGGGGGTACTG GGGA6GACGAUGCGGAUU6GUGAAGGGGGG UACUGCAGACGACUCGCCCGA 0084_ .H32 2945. .H04
G CCTTTCGTTCTGCCCTTCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCU UCCCAGACGACUCGCCCGA 0085. .H32 0080. _HQ4
TTGGGTGTAGGGAGGTACTG GGGAGGACGAUGCGGU UGGG UGUAGGGAGGUACUGCAGACGACUCGCCCGA 0086. .H32 3527. _H04
TAAAGGAGTGGGG GGTCCG GGGAGGACGAUGCGGUAAAGGAGUGGGGAGGLCCGCAGACGACUCGCCCGA 0087. ,H32 3202. „H04
ATTGGTGAAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGUGAAGGGA6GGACUGCAGACGACUCGCCCGA Q088_ H32 2889. .H04
TTTG G G GTTTTCCCG TG CCG GGGAGGACGAUGCGGU UUGGGGUUUUCCCGUGCCGCAGACGACUCGCCCGA 0089. H32 0050. .H04
TTAGGGTGTACGGTGGTGCG GGGAGGACGAUGCGGU UAGGGUG UAGGGUGGUGCGCAGACGACUCGCCCGA 0090. H32
TTGGGTGTAGGGTGGTTCCG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGUUCCGCAGACGACUCGCCCGA 0091„ H32 0388. _H04
G CCTTTCGTTCTGCTCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCUCUCCCCAGACGACUCGCCCGA 0Q92_ .H32 0055. .H04
ATTGGTGGAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGGAGGGAGG UACUGCAGACGACUCGCCCGA 0093.. H32 3733. .H04
TTTGAAGGGTGATGGGTCGG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGUCGGCAGACGACUCGCCCGA 0094. .H32 0150. .H04
CTAAGAGGAGGTGATGGGAG GGGAGGACGAUGCGGCUAAGAGGAGGUGAUGGGAGCAGACGACUCGCCCGA 0095_ .H32
TTTGTAGGGTGATGGGTCCG GGGAGGACGAUGCGGU UUGUAGGGUGAUGGG UCCGCAGACGACUCGCCCGA 0096. H32 0106_ _H04
ATrrTTGGAGAAGGGAGGTG GGGAGGACGAUGCGGAUUUUUGGAGAAGGGAGGUGCAGACGACUCGCCCGA 0097_ _H32
TTTAAAGGGTGATGGGTCCG GGGAGGACGAUGCGGU UUAAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0098.. .H32 012G. . H04
TATACGGCGGTGTGGGGATA GGGAGGACGAUGCGGUAUACGGCGGUGUGGGGAUACAGACGACUCGCCCGA 0099. „H32
TTTGAAGGGTGATGGGGCCG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGGCCGCAGACGACUCGCCCGA 01G0. H32 0110. H04
ATTGGTGAAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGCACUGCAGACGACUCGCCCGA 0101_ ,H32 3575. _H04
TGTAGGAGGAGGAGGTACTG GGGAGGACGAUGCGGUGUAGGAGGAGGAGG UACUGCAGACGACUCGCCCGA 0102. .H32
ATGTATGTTGGGAGCCACGC GGGAGGACGAUGCGGAUGUAU6UUGGGAGCCACGCCAGACGACUCGCCCGA 0103. H32 0434. ,H04
G CCTTTC GTTCTGCCCTCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCUCCCAGACGACUCGCCCGA 0104. .H32 0086. .H04
TATACG GTG GTGTGGGG ATA GGGAGGACGAUGCGGUAUACGGUGG UGUGGGGAUACAGACGACUCGCCCGA 0105. _H32
TTACGGAGGTGAGGGGCAAC GGGAGGACGAUGCGGU UACGGAGG UGAGGGGCAACCAGACGACUCGCCCGA 0106_ .H32
TTTTAAGGGTGATGG6TCCG GGGAGGACGAUGCGGU UUUAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0107_ .H32 0122. Ή04
ATTGGTGAAGGGTGGTACCG GGGAGGACGAU6CGGAUUG6UGAAGGG UGG UACCGCAGACGACUCGCCCGA 01Q8_ ,H32 0808. _H04
TTTGAAGGTTGATGGGTCC6 GGGAGGACGAUGCGGU UUGAAGGUUGAUGGGUCC6CAGACGACUCGCCCGA 0109. .H32 0167. .H04
TTGGGGGTAG GGTGGT ACCG GGGAGGACGAUGCGGU UGGGGGUAGGGUG6UACCGCAGACGACUCGCCCGA 0110_ _H32 0287. -H04
CTGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGCUGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0111 H32 0427 H04
# in H4
HT5 read variable region RNA sequences # in Hi selection selection Selected sequenc
GCCmCGTTCTACCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUACCCUCCCCAGACGACUCGCCCGA G112. .H32 0032, H04
ATTGAAGGGTGATGGGTC.CG GGGAGGACGAUGCGGAUUGAA6GGUGAUGGGUCCGCAGACGACUCGCCCGA 0113. H32 Q187_ H04
TTTGAAGGGTGATGGGTACCG GGGAGGACGAUGCGGUUUGAA6G6UGAUG6GUACCGCAGACGACUCGCCCGA 0114_ _H32 0540_ H04
TTTGAAGGGTGATGGGTCAG GGGAGGACGAUGCGGUU UGAAGGGUGAUG6GUCAGCAGACGACUCGCCCGA 0115. _H32 0143_ H04
ATGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUGGUGAAGGGAGGUACUGCAGACGACUCGCCOGA 0116, _H32 2808_ H04
GCCTTTCGTTCTGCCCrCTC GGGAGGACGAUGCGGGCCUUUCGliUCUGCCCUCUCCAGACGACUCGCCCGA 0117. .H32 0202_ HQ4
TGAGTCGTTCCCTTCGTCCC GGGAGGACGAUGCGGUGAGUCGUUCCCUUCGUCCCCAGACGACUCGCCCGA 0118_ .H32 0027_ H04
TTTGAAGGGTGAAGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAAGGGUCCGCAGACGACUCGCCCGA 0119. _H32 0166_ H04
GACTAGCGATCTGTTACGCA GGGAGGACGAUGCGGGACUAGC6AUCUGUUACGCACAGACGACUCGCCCGA 0120_ _H32 0177_ H04
TTTGCAGG GTGATG G GTCCG GGGAGGACGAUGCGGUUUGCAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0121. _H32 0129. _H04
TTTGAAGGGTGATGGGTACG GGGAGGACGAUGC6G UUUGAAGGGUGAUGGG UACGCAGACGACUCGCCCGA 0122. .H32 0138_ .H04
TCCTGTCGTCTGTTCTTCCC GGGAGGACGAUGCGG UCCUGUCGUCUGUUCUUCCCCAGACGACUCGCCCGA 0123. .H32 0121_ .H04
•J\ ATTGGAGGTGGTGGGTTCCG GGGAGGACGAUGCGGAUUGGAGG UGGUGGGUUCCGCAGACGACUCGCCCGA 0124. _H32 1072_ _HQ4
ATC6GTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUCGGUGAAGGGAGGUACUGCA6ACGACUCGCCCGA 0125. _H32 3667_ H04
TCTGTGTG GGATGTG GCCC GGGAGGACGAUGCGGUCUGUGUGGGAUGUGGCCCCAGACGACUCGCCCGA 0126. _H32 0231. _H04
TTTGAAG G GTG ATGG GTCCT 6GGAGGACGAUGCGG UUUGAAGGGUGAUGGG UCCUCAGACGACUCGCCCGA 0127. .H32 0192_ H04
GCCTTTCGTTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UGCCCUCCCCAGACGACUCGCCCGA 0128. .H32 0164„ ,H04
TTTGAAG G TG ATGG GTCCG GGGAGGACGAUGCGGUUUGAAGG UGAUGGGUCCGCAGACGACUCGCCCGA 0129. .H32 Q246_ .H04
TTGGGTGTAGGGTG6CACCG GGGAGGACGAUGCGG UU6GGUGUA6GGUGGCACCGCAGACGACUCGCCCGA 0130. _H32 0391_ _H04
GTCCTGTCGTCTTACGTCCC GGGAGGACGAUGCGGGUCCUG UCGUCUUACGUCCCCAGACGACUCGCCCGA 0131. _H32 0044_ H04
ATTGGTGAGGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAGGGGAGGUACUGCAGACGACUCGCCCGA 0132. „H32
GCCTTCGTTTGGTTCGTCCC GGGAGGACGAUGCGGGCCUUCGUUUGGUUCGUCCCCAGACGACUCGCCCGA 0133. .H32 0328_ .H04
ATTGGTGAAGGGAGTTACTG GGGAG6ACGAUGCGGAUUGGUGAAGGGAGU UACUGCAGACGACUCGCCCGA 0134. .H32
TTGGGTGTGGGGTGGTACCG GGGAGGACGAUGCGGUUGGGUGUGGGG'JGGUACCGCAGACGACUCGCCCGA 0135. _H32 0390_ .H04
ATTGGTGAAGGGAGGAACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGAA.CUGCAGACGACUCGCCCGA 0136. _H32 4523_ .H04
TTTGGTGTAGG6TGGTACCG GGGAGGACGAUGCGGUUUGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0137. _H32 0277_ H04
TTGGGTGTAG6GTGGGACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGGACCGCAGACGACUCGCCCGA 0138. .H32 0412_ .H04
CTGTCTACCTTCTACTGCCG GGGAGGACGAUGCGGCUGUCUACCUUCUACUGCCGCAGACGACIJCGCCCGA 0139 H32 0248 H04
# irt H4
HTS read variable region NA sequences tt in H3 selection selection Selected sequenc
TTTGAC6GGTGATGGGTCCG GGGAGGACGAUGCGGU UUGACGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0140. H32 0185_ M04
GCCTTTCGTTCTGCCTCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCUCCCCCAGACGACUCGCCCGA 0141„ ,H32 0074_ HG4
ATTGGTGAAGGGTGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGG UGGUACUGCAGACGACUCGCCCGA 0142. .H32
GCCTfCG TTCTGACCTCCC GGGAGGACGAUGCGGGCCU UCGUUCUGACCUCCCCAGACGACUCGCCCGA 0143. _H32 2045. H04
TTG G GTG C AG G GTG GTACCG GGGAGGACGAUGCGGUUGGGUGCAGGGUGGUACCGCAGACGACUCGCCCGA 0144_ _H32 0401_ H04
ACTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGACUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0145. H32 KU7
TCCCTTTCGTCTCGCGTCCC GGGAGGACGAUGCGGUGCGUU UCGUCUCGCG UCCCCAGACGACUCGCCCGA 0146. .H32 1227_ H04
TCTGTACAGTCTCCCATGTG GGGAGGACGAUGCGGUCUGUACAGUCUCCCAUG JGCAGACGACUCGCCCGA 0147. „H32 0285_ H04
GCTTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCUUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0148. .H32 0047_ H04
TTTGAAGGGTGATGGGTGCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUGCGCAGACGACUCGCCCGA 0149. _H32 0209_ H04
GCCTTTCGTTCTGCCTTCCC GGGAGGACGAUGCGGGCCUUUCGU UCUGCCUUCCCCAGACGACUCGCCCGA 0150. .H32 0108_ H04
GCCTTTCGTTCTTCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUCCCUCCCCAGACGACUCGCCCGA 0151. _H32 0037. .H04
TCCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGUCCUUUCGU UCUGCCCUCCCCAGACGACUCGCCCGA 0152. .H32 0024_ K04
TCGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUCGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0153. _H32 0525. H04
TTGGGTGTAGAGTGGTACCG GGGAGGACGAU6CGGUUGGGUGUAGAGUG6UACCGCAGACGACUCGCCCGA 0154. _H32 0627_ .H04
TTGG ATGTAGG GTG GTACCG GGGAGGACGAUGCGGUUGGAUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0155. H32 G700. H04
TTTGAAGGGGGATGGGACCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGACCGCAGACGACUCGCCCGA 0156. _H32 0596. H04
GCCTTTC6TTCTGG CCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGGCCUCCCCAGACGACUCGCCCGA 0157. .H32 0673. _K04
ATTG GAG AAGGGAG GTACTG GGGAGGACGAUGCGGAUUGGAGAAGGGAGGUACUGCAGACGACUCGCCCGA 0158. .H3 4883. K04
ATTG G G TG TAG 6 G AGTTCCG GGGAGGACGAUGCGGAUUGGGUGUAGGGAGUUCCGCAGACGACUCGCCCGA 0159. _H32 2134. .H0
TGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0160. _H32 0443_ _H04
TATTG G AGGTG TAG G G TCCG GGGAGGACGAUGCGGUAU UGGAGGUGUAGGGUCCGCAGACGACUCGCCCGA 0161. .H32 1550.. .H04
TTG GGTGTAGG GTAGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUAGUACCGCAGACGACUCGCCCGA 0162. .H32 0933. H04
GCATTTC6TTCT6ACCTCCC GGGAGGACGAUGCGGGCAUUUCGU UCUGACCUCCCCAGACGACUCGCCCGA 0163. .H32
G CTTTCGTTCTG CCCTCCC GGGAGGACGAUGCGGGCUUUCGU UCUGCCCUCCCCAGACGACUCGCCCGA 0164„ .H32 0093. H04
GCCTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCGUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0165. .H32 0097_ .H04
TTG GGTGTAGTG TGGTACCG GGGAGGACGAUGCGGU UGGGUGUAGUGUGGUACCGCAGACGACUCGCCCGA 0166. H32 0705. _H04
TATGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUAUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0167. _H32 0229. _H04
# m H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATTGGTAAAGGGAGGTACTG 6GGAGGACGAUGC6GAUUGG UAAAGGGAGGUACUGCAGACGACUCGCCCGA 0168, _H32
TTAG G TGTAG G GTG GTACCG GGGAGGACGAUGCGGUUAGG UGUAGGGUGGUACCGCAGACGACUCGCCCGA 0169_ .H32 0323. H04
TTGGGTGTAGGATGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGAUGGUACCGCAGACGACUCGCCCGA 0170_ H32 0780. H04
TTG G G TG TAG G GTG GTACCC GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUACCCCAGACGACUCGCCCGA 0171. .H32 0144. H04
TTGGGTG TAAGGTG GTACCG GGGAGGACGAUGCGG UUGGGUGUAAGGUGGUACCGCAGACGACUCGCCCGA 0172. _H32 0704_ .H04
GCCCTTCGTTCTGCCCTCCC GGGAGGACGAUGCGG6CCCUUCGUUCUGCCCUCCCCAGAC6ACUCGCCCGA 0173. „H32 0171_ H04
TTTGAAGGCTGATGGGTCCG GGGAGGACGAUGCGG UU UGAAGGCUGAUGGGUCCGCAGACGACUCGCCCGA 0174 „H32 0405_ H04
G CCTTTCGTTCTGTCCTCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGUCCUCCCCAGACGACUCGCCCGA 0175_ .H32 0204. H04
TTTGAAGG6TGATGTCCG GGGAGGACGAUGCGG UUUGAAGGGUGAUGUCCGCAGACGACUCGCCCGA Q176. .H32 0396. H04
ATGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGAUGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0177. .H32 1054_ „H04
AGTCTTGTCGTTTGTGGCGC GGGAGGACGAUGCGGAGUCUUGUCGUU UGUGGCGCCAGACGACUCGCCCGA 0178. .H32 0224_ H04
TTTG AAGG GTG ATG CGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGCGUCCGCAGACGACUCGCCCGA 0179_ _H32 0369. H04
TGTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGG UGUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0180. .H32 0343. H04
•M
TTTGAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0181. H32 0321. HQ4
TTTGAAGGGTCATGGGTCCG 6GGAGGAC6AUGCGG UUUGAAG66UCAUGGGUCCGCAGACGACUCGCCCGA 0182. _H32 0365. H04
G CCTTCTTCTG GTTCG TCCC GGGAGGACGAUGCGGGCCUUCUUCUGGUUCG UCCCCAGACGACUCGCCCGA 0183. .H32 0125. _H04
TTTCAAG GGTG ATGG GTCCG GGGAGGACGAUGCGGUUUCAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0184. .H32 0373. H04
G C CTTTCGTTCTGCC CTC CG GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCUCCGCAGACGACUCGCCCGA 0185. .H32 0422. .H04
G CCTTTCGTTCTG ACCTCC GGGAGGACGAUGCGG6CCUUUCGU UCUGACCUCCCAGACGACUCGCCCGA 0186. _H32 5247. ^HQ
TTGG6TGTTGGGTGGTACCG GGGAG6ACGAUGCGGUUGG6UGUUGGGUGGUACCGCAGACGACUCGCCCGA 0187. _H32 0698. _H04
TTGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUUGGUGUAGGGUGGUACCGCAGACGACLiCGCCCGA 0188. _H32 0535. _H04
TTTGAAGGGTGATGGGCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGCCGCAGACGACUCGCCCGA 0189. H32 0901. .H04
GCCTTTCGTTCTGTTCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGUUCUCCCCAGACGACUCGCCCGA 0190. .H32 0114. .H04
ATTGGTGTAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGUAGGGAGGUACUGCAGACGACUCGCCCGA 0191. _H32 4939 _H04
TTG G G TG TAG G GTG G TACCA GGGAGGACGAUGCGGU UGGG UGUAGG6UGGUACCACAGACGACUCGCCCGA 0192. _H32 0752. _H04
TTAAGGTGGTGTAGGGGCCG GGGA6GACGAUGCGGU UAAGG UG6UGUAGGGGCCGCAGACGACUCGCCCGA 0193. .H32 1126. _H04
TTG AGTGTAG G GTG GTACCG GGGAGGACGAUGCGGUUGAGUGUAGGGUGGUACCGCAGACGACULGCCCGA 0194. .H32 0926. .H04
TTGGGTGGAG6GTGGTACCG GGGAGGACGAUGCGGU UGGG UGGAGGGUGGUACCGCAGACGACUCGCCCGA 0195 H32 0619 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
GCCTTTCGTTCTGCCCTCCT GGGAGGACGAUGCGGGCCUUUCGU UCUGCCCUCCUCAGACGACUCGCCCGA 0196. _H32 0295. _HQ4
G CCTTTCGTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUCUGCCCUCCCCAGACGACUCGCCCGA 0197. _H32 0424. HQ4
TTG G G TGT ATG GTGGTACCG GGGAGGACGAUGCGGUUGGG UGUAUGGUGGUACCGCAGACGACUCGCCCGA 0198. _H32 0659. _H04
GCCTTTCGTTCTGCCCCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCCCCCCAGACGACUCGCCCGA 0199. _H32 0197. _H04
G C CTTTCG TTCTG ACCTCCA GGGAGGACGAUGCGGGCCUUUCGUUCUGACCUCCACAGACGACUCGCCCGA 0200. _H32
ATTG GTG AAG G GAAGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAAGUACUGCAGACGACUCGCCCGA 0201. .H32
TTGGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGU LIGGG UGAAGGGAGG UACUGCAGACGACUCGCCCGA 0202. .H32 5004. _HG4
ATTGGGGAAGGGAGGTACTG GGGAoGACGAUGCGGAUUGGGGAAGGGAGG UACUGCAGACGACUCGCCCGA 0203. _H32 5131. .H04
CCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGCCUU UCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0204. _H32 0079. _H04
GCCCTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCCU LfCGUUCUGACCUCCCCAGACGACUCGCCCGA 0205. .H32 1999. .H04
ATTGGTGAAGGGAGGTT6 GGGAGGACGAU6CGGAUUG6UGAAGGGAGGUUGCAGAC6ACUCGCCCGA 0206. .H32
GCCTFTCGTTCTGCCCTCCA 6GGAGGACGAUGCGGGCCUUUCGUUCUGCCCUCCACAGACGACUCGCCCGA 0207. .H32 0215. „H04
GCCATTCGTCTTTTCGTCCC GGGAGGACGAUGCGGGCCAUUCGUCUUUUCGUCCCCAGACGACUCGCCCGA 0208. .H32 1573. _H04
CTGT6AGGAGCAGGGTGGTA GGGAGGACGAUGCGGCUGUGAGGAGCAGGGUGGUACAGACGACUCGCCCGA 0209. .H32
TTGGGTAGGGT6GTACCG GGGAGGACGAUGCGGUUGGGUAGGGUGGUACCGCAGACGACUCGCCCGA 0210, .H32 0411. .H04
TTGGGTGTAGGGTGATACCG GGGAGGACGAUGCGGUUGGGUGUAGGG UGAUACCGCAGACGACUCGCCCGA 0211. .H32 1030. _H04
TTTGAAGGGTGAGGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAGGGGUCCGCAGACGACUCGCCCGA 0212. H32 0357. .H04
ATTGGTGAAGGGAGATACTG GGGA6GACGAUGCGGAUUGGUGAAGGGAGAUACUGCAGACGACUCGCCCGA 0213. H3' 2
TTTG AAG GGTG ATGGGTG GGGAGGACGAUGCGGU UU6AAGGGUGAUGGGUGCAGACGACUCGCCCGA 0214. _H32 0709. _H04
TAGGG GTGTGG G GTGTTATG GGGAG6ACGAUGC6GUAGGGGUGUGGG6UGUUAUGCAGACGACUCGCCCGA 0215_ _H32
ATTGGTGATGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAUGGGAGG UACUGCAGACGACUCGCCCGA Q216„ _H32
ATTGGTGAAAGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAAGGAGGUACUGCAGACGACUCeCCCGA 0217. „H32
TTTG AAG GGTG ATGG GTC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCCAGACGACUCGCCCGA 0218. _H32 0604. .H04
GCCTCTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCUCUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0219. .H32 2995. H04
ATTGGTGAAGGGAGCTACTG GGGAGGACGAUGCGGAUUG6UGAAGGGAGCUACUGCAGACGACUCGCCCGA 0220. .H32
ATTG 6 TG A AGG AAG G T ACTG GGGAGGACGAUGCGGAUUGGUGAAGGAAGGDACUGCAGACGACUCGCCCGA 0221. H32
ATTG ATG AAGGGAGGTACTG GGGAGGACGAUGCGGAUUGAUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0222. .H32
TTGGGTGTAGGGTGGAACCG GGGAGGACGAUGCGGUUGGGUGUAGGG UGGAACCGCAGACGACUCGCCCGA 0223 H32 0877 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection
CTG TTTG TAAG G G AG GT6GT GGGAGGACGAUGCGGCUGU UUGUAAGGGAGGUGGUCAGACGACUCGCCCGA 0224. .H32
TTG GGTGTAGG GTG CTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGCUACCGCAGACGACUC.GCCCGA 0225_ H32 1102. _H04
GCCTTTCGTTCTGACCTTCC GGGAGGACGAUGCGGGCCUUUCGUUCUGACCU UCCCAGACGACUCGCCCGA Q226_ H32 39D1. _H04
G7TG G T GAAG G GAG GTACCG GGGAGGACGAUGCGGGUUGGUGAAGGGAGGUACCGCAGACGACUCGCCCGA G22?_ H32 0759. .H04
ATTAGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUUAGUGAAGGGAGG UACUGCAGACGACUCGCCCGA 0228_ H32
TTGGGTGTAGGGTTGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUUGUACCGCAGACGACUCGCCCGA 0229. H32 0858. K04
ATTGGTGAAGAGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGAGAGG UACUGCAGACGACUCGCCCGA 0230_ H32
TTGGGTGAAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGUGAAGGGUGGUACCGCAGACGACUCGCCCGA 0231. H32 0968. _H04
TTGGGTGTAGGGTGGTACGG G6GAGGACGAUGCGGUU6GGUGUAGGGUGGUACGGCAGACGACUCGCCCGA 0232_ H32 1288. .H04
GCCTTTCGCTCTGCCCTCCC GGGAGGACGAUGCGGGCCUU UCGCUCUGCCCUCCCCAGACGACUCGCCCGA 0233. .H32 0218. ,H04
TTGGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGUUGGGUGAAGGGAGGUACCGCAGACGACUCGCCCGA 0234. H32 2753. .R04
AATGGTGGAGTAGGGATACG GGGA6GACGAUGCGGAAUGGUGGAGUAGGGAUACGCAGACGACUCGCCCGA 0235, H32
TGAAGGAGT6GGGAGGTCCG GGGAGGACGAUGCGGUGAAGGAGUGGGGAGGUCCGCAGACGACUCGCCCGA 0236_ H32
GCCTCTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUCUCG UUCUGCCCUCCCCAGACGACUCGCCCGA 0237. H32 0232. „H04
TATTGGAGGTGTAGGGGCCG GGGAGGACGAUGCGGUAUUG AGGUGUAGGGGCCGCAGACGACUCGCCCGA 0238. H32 0649. _H04
TTGGGTATAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGUAUAGGGUGGUACCGCAGACGACUCGCCCGA 0239. H32 0838. _H04
TTTAA6GGTGATGGGTCCG GGGAGGACGAUGC6GUUUAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0240. H32 0425. H04
TCCTGTCGTCCGTTCGTCCC GGGAGGACGAUGCGGUCCUGUC6UCCGUUCGUCCCCAGACGACUC6CCCGA 0241_ H32 0168. _H04
GCTTTTCG TTCTG ACCTC CC GGGAGGACGAUGCGGGCUUUUCGU UCUGACCUCCCCAGACGACUCGCCCGA 0242.. .H32 1916. H04
ATTG GTG AAGTG AGG TACTG GGGAGGACGAUGCGGAUUGGUGAAGUGAGGUACUGCAGACGACUCGCCCGA 0243. .H32
GCCTTTCGTTCTGCCCTTTC GGGAGGACGAUGCG6GCCUU UCGUUC.UGCCCUUUCCAGACGACUCGCCCGA 0244_ H32 0530. .H04
GCCTTTCGTTCTGCCATCCC GGGAGGACGAUGCGGGCCUU UCGUUCUGCCAUCCCCAGACGACUCGCCCGA 0245. H32 0259. .H04
ATTGGIGAATGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAUGGAGGUACUGCAGACGACUCGCCCGA 0246. JH32
ATTG GTG AAGG G AG GTTCTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUUCUGCAGACGACUCGCCCGA 0247. H32 2373. _H04
ATTGGTGAAGGGAGGGACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGGACCGCAGACGACUCGCCC6A 0248_ H32 2254. _H04
TTGGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGU UGGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0249. H32 0359. _H04
ATTGGTGGAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGUGGAGGGAGG UACCGCAGACGACUCGCCCGA Q2S0. H32 3200. _H04
TTTGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGU UUGGUGAAGGGAGGUACCGCAGACGACUCGCCCGA 0251 H32 2032 H04
# in H4
HTS read variable region R A sequences # in H3 selection selection Selected sequenc
ATTCJGTGAAGGGAGGTACGG GGGAGGACGAUGCGGAUUGGUGAAGGGAGG UACGGCAGACGACUCGCCCGA 0252. H32
TTTGAAGGGTGATAAGTCC6 GGGAGGACGAUGCGGUUUGAAGGGUGAUAAGUCCGCAGACGACUCGCCCGA 0253_ H32 0449. H04
TTTGAACGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAACGGUGAUGGGUCCGCAGACGACUCGCCCGA 0254. .H32 0542. j-104
G'TCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGUCU UUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0255. H32 0220. H04
ACCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGACCUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0256. .H32 0142. ,H04
ATTGGTGAAGGGAGGTGCCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUGCCGCAGACGACUCGCCCGA 0257_ _H32 0701. .H04
TTGGGTTTAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGUUUAGGGUCGUACCGCACACGACUCGCCCCA 0258. 32 0980. _H04
TTTGAAGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGAUGGGUCCGCAGACGACUCGCCCGA 0259, H32 0438. _H04
GCCTTTCGTTCTGCCCTCAC GGGAGGACGAUGCGGGCCU UUCGUUCUGCCCUCACCAGACGACUCGCCCGA 0260_ .H32 0356. .H04
TTTGAAGCGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGCGUGAUGGGUCCGCAGACGACUCGCCCGA 0261. .H32 0542. .H04
TTTGAAGGGGGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGG UGGGUCCGCAGACGACUCGCCCGA 0262. .H32 0682. _H04
CAGAGGAGAAGGGAGGTACTG GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUACUGCAGACGACUCGCCCGA 0263. H32
TTGGTTGTAGGGTGGTACCG GGGAGGACGAUGCGGUUGGUUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0264. H32 0978. _H04
90
ATTGGTGAAGGGATGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAUGUACUGCAGACGACUCGCCCGA 0265. H32
ATTGGTGAAGGGAGGTATTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUAUUGCAGACGACUCGCCCGA 0266. .H32
TTGTGTGTAGGGTGGTACCG GGGAGGACGAUGCGGU UGUGUGUAGGGUGGUACCGCAGACGACUCGCG'CGA 0267_ H32 0961. _H04
GCCTTTCGTTTTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUUUGCCCUCCCCAGACGACUCGCCCGA 0268_ H32 0210. H04
GCCTTTCGTTCTGCCCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCCCCCAGACGACUCGCCCGA 0269. _H32 0305. .H04
G CCTTTC G TTCTG ATCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGAUCUCCCCAGACGACUCGCCCGA 0270.. H32 2666. _H04
TTTGAAGGGTGATCGGTCCG GGGAGGACGAUGCGGUUUGAA6GGUGAUCG6UCCGCAGACGACUCGCCCGA 0271. .H32 0541. _H04
GCCTTTCGTTCTGCACTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCACUCCCCAGACGACUCGCCCGA 0272. _H32 0333. _H04
ATTGGCGAAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGCGAAGGGAGGUACCGCAGACGACUCGCCCGA 0273. _H32 2372. .H04
AGGTGTAGGGGGGTGTACCG GGGAGGACGAUGCGGAGGUGUAGGGGGGUGUACCGCAGACGACUCGCCCGA 0274. _H32
ATTG GIG AAGG G AG GTACTA GGGAGGACGAUGCGGAUUG6UGAAGGGAGGUACUACAGACGACUCGCCCGA 0275. H32
TTGGGTGTAGG GGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGUUGGUACCGCAGACGACUCGCCCGA 0276. H32 1516. _H04
TTAG AAG G GTG ATG GGTCCG GGGAGGACGAUGCGGUUAGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0277. H32 0532. H04
ATTGGTGAAGGGAGGTACAG GGGAGGACGAUGC6GAUUGGUGAAGG6AGGUACAGCAGACGACUCGCXCGA 0278. H32
GCCTTTCGTTCTTCTCCC GGGAGGACGAUGCGGGCCUU UCGUUCUUCUCCCCAGACGACUCGCCCGA 0279. 32 1051. _H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
GTGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGGUGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0280. ..H32 2047_ H04
TTGGGTGTAGGGTGGTATCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUAUCGCAGACGACUCGCCCGA 0281. .H32 1368_ H04
G CCTTTCGTTTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UUGCCCUCCCCAGACGACUCGCCCGA 0282. _H32 0348_ ,H04
GCCTTTCGGTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCGGUCUGCCCUCCCCAGACGACUCGCCCGA 0283. .H32 0376_ .H04
TTG G GTGTAGG GTGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUG UACCGCAGACGACUCGCCCGA 0284. .H32 0903. .H04
G C CTTTCGTCTG ACCTC CC GGGAGGACGAUGCGGGCCUUUCGUCUGACCUCCCCAGACGACUCGCCCGA 0285. _H32 3161_ H04
TGTAGGAGGAGGAGGGACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGGGACCGCAGACGACUCGCCCGA 0286. _H32
GCCTTTCGTTCTGCCCTGCC GGGAGGACGAUGCGGGCCUUUCGU UCUGCCCUGCCCAGACGACUCGCCCGA 0287. .H32 0389. ,H04
ATTGGTGAAGGGAGGCACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGCACCGCAGACGACUCGCCCGA 0288. .H32 2621. .H04
6CCTTTCGTTCTGCCCACCC GGGAG6ACGAUGCGGGCCUUUCGUUCUGCCCACCCCAGACGACUCGCCCGA 0289. .H32 0480_ .H04
GCTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCUU UCGUUCUGACCUCCCCAGACGACUCGCCCGA 0290. H32 3545_ H04
TGTAGGAGGAGGAGGTGCCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGGUGCCGCAGACGACUCGCCCGA 0291. H32 4811_ H04
ATTGGTTAA GGGAG GTACTG GGGAGGACGAUGCGGAUUGGU UAAGGGAGGUACUGCAGACGACUCGCCCGA 0292. .H32 4995„ H04
GCCTTTCGTTCTGCCCTACC GGGAGGACGAUGCGGGCCU UUCGUUCUGCCCUACCCAGACGACUCGCCCGA 0293. .H32 0419. H04
CTTGGTGAAGG GAG GTACTG GGGAGGACGAUGCGGCUUGGUGAAGGGAGG UACUGCAGACGACUCGCCCGA 0294. _H 2
ATTGGTGAAGGGAGGTTCCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUUCCGCAGACGACUCGCCCGA 0295. . H32 2492_ _H04
TCCTGTCGTCTGCTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGCUCGUCCCCAGACGACUCGCCCGA 0296. .H32 0025_ H04
ATTGGTGAAGGTAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGUAGGUACUGCA6ACGACUCGCCCGA 02S7. _H32
TTGGGTGTAG G GTG GTACAG G6GAGGACGAUGCGGUUGGGUGUAGGGUG6UACAGCAGACGACUCGCCCGA 0298. .H32 1554. _H04
TTG G GTGT AG GTGGTACCG G6GAGGACGAUGCGGUUGGGUGUAGGUGGUACCGCAGACGACUCGCCC6A 0299. .H32 1351. „H04
TTGGGAGTAGGGTGGGACCG GGGAGGACGAUGCGGUUGGGAGUAGGG UGGGACCGCAGA GACUCGCCCGA 0300. H32 1116. H04
TTGGGTGTAGGCTGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGCUGGUACCGCAGACGACUCGCCCGA 0301. .H32 2422. .H04
GCCTTTCGTTCTGCGCTCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGCGCUCCCCAGACGACUCGCCCGA 0302. ,H32 0358. .H04
GCATTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCAUUUCGU UCUGCCCUCCCCAGACGACUCGCCCGA 0303. _H32 G395_ .H04
ATTGGTGAGGGGAGGTACCG GGGAGGACGAUGCGGAUUGGUGAGGGGAGG UACCGCAGACGACUCGCCCGA 0304. _H32 2595. _H04
GCCTTTCGTTCTGACCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGACCCCCCAGACGACUCGCCCGA 0305. _H32 5249. .H04
TGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0306. _H32 0519. _H04
GCCTTTCGATCTGCtCTCCC GGGAGGACGAUGCGGGCCU U UCGAUCUGCCCUCCCCAGACGAC UCGCCCGA 0307. _H32 0410. _H04
# in H4
NTS read variable region NA sequences U in H3 selection selection Selected sequenc
CCCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGCCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0308. .H32 0123. _H04
GTAGGTGGAGTAGGGGTCCG GGGAGGACGAUGCGGGUAGGUGGAGUAGGGGUCCGCAGACGACUCGCCCGA 0309_ _H32
ATTG GTG A AG G GAG GTACTT GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACUUCAGACGACUCGCCCGA 0310. .H32
GCCTTTTGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUUGUUCUGCCCUCCCCAGACGACUCGCCCGA 0311_ H32 0266. HQ4
GCCTTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0312. .H32 0260. _H04
TTTGGAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGGAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0313, H32 1108. .H04
TTGAAGGGTGATGGTACCG GGGAGCACCAUGCGCUUUGAAGGOUGAUGGUACCGCA.GACGACUCGCCCGA 0314_ _H32 1815. H04
GCCTTTCGTCCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCGUCCUGCCCUCCCCAGACGACUCGCCCGA 0315. .H32 0335. _H04
GCCTTTCATTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCAUUCUGCCCUCCCCAGACGACUCGCCCGA 0316. .H32 0274. .H04
GCCTTTCGTTCT6ACTTCCC GGGAGGACGAUGCGGGCCUUUCGU UCUGACUUCCCCAGACGACUCGCCCGA 0317. H32 3526. .H04
TTTGAAGGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGG6UGAUGGGUCCGCAGACGACUCGCCCGA 0318. H32 0477_ _H04
GCCTTTCGTTCTCCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUCCCCUCCCCAGACGACUCGCCCGA 033.9. _H32 02.49. .H04
TTG'GGTCTAGGGTGGTACCG GGGAGGACGAUGCGGU UGGGUCUAGGGUGGUACCGCA.GACGACUCGCCCGA 0320. H32 1945. .H04
o
GCCTTTC G CTCTGA CCTCCC GGGAGGACGAUGCGGGCCUUUCGCUCUGACCUCCCCAGACGACUCGCCCGA 0321, .H32 2804. .H04
GCCT'tTCGTTCTAACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUAACCUCCCCAGACGACUCGCCCGA 0322. _H32 2785. ,H04
6CCTTTCGTTCTGACTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGACUCCCCAGACGACUCGCCCGA 0323. _H32 3900. _H04
TTGGCTGTAGGGTGGTACCG GGGAGGACGAUGC6GU UGGCUGUAGGGUG6UACCGCAGACGACUCGCCC.GA 0324. H32 3664. _H04
ATTGGTGAAGG GGG GTACCG GGGAGGACGAUGCGGAUUGGUGAAGGGGGGUACCGCAGACGACUCGCCCGA 0325. H32 2450. -H04
GCCTTTCGTTCTC C.CTCC C G6GAGGACGAUGCGGGCCUUUCGUUCUCCCUCCCCAGACGACUCGCCCGA 0326. U32 0088. _H04
GCCTTTCGTCCTGACCTCCC GGGAGGACGAUGCGGGCCUUUCGUCCUGACCUCCCCAGACGACUCGCCCGA 0327. _H32 2864. _H04
TATTGGAGGTGTAGGTACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAGG UACCGCAGACGACUCGCCCGA 0328. _H32
TCCTGTCGTCTCTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUCUUCGUCCCCAGACGACUCGCCCGA 0329. .H32 0208. .H04
TTGGGTGTAGGGTGTTGCCG GGGAGGACGAUGCGGUUGGGUGUAGGGUG UUGCCGCAGACGACUCGCCCGA 0330. _H32 0745. .H04
TGGTGTGTCAAGGTACGCCC GGGAGGACGAUGCGGUGGUGUGUCAAGGUACGCGCCAGACGACUCGCCCGA 0331. .H32 0813. .H04
ATGGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUGGGUGAAGGGAGGUACUGCAGACGACUCGGCCGA 0332. _H32
TCCTGTCGTCTGTTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUGUCCCCAGACGACUCGCCCGA 0333. _H32 0035. _H04
TTFG AAGGGTGATGGGTCC GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGUCC.CAGACGACUCGCCCGA 0334. _H32 0730. .H04
TG G G G TGT AG G GTG GTAC CG GGGA6GACGAUGCGGUGGGGUGUAGGG UGG UACCGCAGACGACUCGCCCGA 0335 H32 1953 H04
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TTTGGAGGGGGATGGGACCG GGGAGGACGAUGCGGUUUGGAGGGGGAUGGGACCGCAGACGACUCGCCCGA 0336. H32 2263_ _H04
G CCTTTCGTTC AG CCCTCCC GGGAGGAC6AUGCGGGCCUUUCGUUCAGCCCUCCCCAGACGACUCGCCCGA 0337. H32 0494_ H04
TTG GGTGTAGG GTG GTACCT GGGAGGACGAUGCGGUUGGG UGUAGGGUGGUACCUCAGACGACUCGCCCGA 0338.. H32 2253. .H04
TTTG AAGGGTG ATG GGTTTG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGU UUGCAGACGACUCGCCCGA 0339. H32 092S_ H04
ACCTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGACCU UUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0340_ H32 3231 . .H04
G CCTTTCGTTCTGA C CTCCG GGGAGGACGAUGCGGGCCU UUCGUUCUGACCUCCGCAGACGACUCGCCCGA D341_ .H32
TCCTGTTGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUUGUCUGUUCGUCCCCAGACGACUCGCCCGA 0342. H32 0087_ .H04
GCCTTTCGTTCTGACCTCAC GGGAGGACGAUGCGGGCCU UUCGU UCUGACCUCACCAGACGACUCGCCCGA 0343_ H32 511Q_ H04
GCCTTTCGTTCCGCCCTCCC GGGAGGACGAUGCGGGCC'J UUCGU UCCGCCCUCCCCAGACGACUCGCCCGA Q344_ H32 0435_ H04
GCCTTTCTTTCTG CCCTCCC GGGAGGACGAUGCGGGCCU UUCUUUCUGCCCUCCCCAGACGACUCGCCCGA 0345. ,H32 0279. _H04
GCCTTTCGTTCTGACTCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGACUCCCCCAGACGACUCGCCCGA 0346_ .H32 2782. _H04
ATAGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUAGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0347. .H32 4185_ H04
ATTG GTGAAG G GCG GTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGCGGUACUGCAGACGACUCGCCCGA 0348_ H32
TTGGGTGTAGGGTGGTCCCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUCCCGCAGACGACUCGCCCGA 0349, _H32 2313_ .H04
G CCTTTCGTTATGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UAUGCCCUCCCCAGACGACUCGCCCGA 0350_ _H32 0474. .H04
ATTTGTGAAG G 6 AG GTACTG GGGAGGACGAUGCGGAUUUGU6AAGGGAGGUACUGCAGACGACUCGCCCGA 0351. .H32
ATCTTACACT6CAGATACCC GGGAGGACGAUGCGGAUCUUACACUGCAGAUACCCCAGACGACUCGCCCGA 0352. H32
ATTGTTGAAGGGAG GTACTG GGGAGGACGAUGCGGAUUGUUGAAGGGAGGJACUGCAGACGACUCGCCCGA Q353. . H32
TTTGAAGGGGGGT6GGACCG GGGAGGACGAUGCGGUUUGAAGGGGGGUGGGACCGCAGACGACUCGCCCGA 0354_ _H32 1844. .H04
G CCTTCCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUCCGUUCUGCCCUCCCCAGACGACUCGCCCGA 03S5_ .H32 0423_ H04
TCCTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGUCCUUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0356_ .H32 1071. _H04
GCCTTTCGTTCTCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUCCUCCCCAGACGACUCGCCCGA 0357. _H32 0353. _H04
TTGGGAGGAGGGTGGGACCG GGGAGGACGAUGCGGUUGGGAGGAGGG UGGGACCGCAGACGACUCGCCCGA 0358. „H32 1649. _H04
CGTGGTGGTGAAGGGGTACG GGGAGGACGAUGCGGC6UGGUGGUGAAGGGGUACGCAGACGACUCGCCCGA 0359. .H32
ATTGTGAAGGGAGGTACT6 GGGAGGACGAUGCGGAUUGUGAAGGGAGGUACUGCAGACGAC1JCGCCCGA 0360. .H32
CTTGAAGGGGGATGGGTCCG GGGAGGACGAUGCGGCUUGAAGGGGGAUGG6UCCGCAGACGACLJC6CCCGA 0361. H32 0644. _H04
ATTGGTGAAGGGAGGAACCG GGGAGGACGAUGCGGAUU6GUGAAGGGAGGAACCGCAGACGACUCGCCCGA 0362. _H32 3316. H04
TTTGAAGGGT6ATTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUUGGGUCCGCAGAC6ACUCGCCCGA 0363 H32 0221 H04
# in H4
HIS read variable region RNA sequences tt in H3 selection selection Selected sequenc
GCCTTTCGTTCTGACCCCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGACCCCCCCA6ACGACUCGCCCGA 0364. _H32 3556_ .H04
G CCATTCG TTCTG CCCTCCC GGGAGGACGAUGCGGGCCAUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0365. .H32 0429_ .H04
TTTTGAAGGGTGATGGGTCCT 6GGAGGACGAUGCGGUU UUGAAGGG UGAUGGGUCCUCAGACGACUCGCCCGA 0366_ _H32 0874_ .H04
G CCTTTCGTTCTG ACATCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGACAUCCCCAGACGACUCGCCCGA 0367. .H32 4060_ HQ4
'I'AAGGTGGTGATGGGATTCG GGGAGGACGAUGCGGUAAGGUGGUGAUGGGAUUCGCAGACGACUCGCCCGA 0368. .H32
AGTTGGAGAAGGGTGGTCTG GGGAGGACGAUGCGGAGUUGGAGAAGGGUGGUCU6CAGACGACUCGCCCGA 0369, _H32
TTTGG AGG AG6GTGG G ACCG GGGAGGACGAUGCGG UU UGGAGGAGGGUGGGACCGCAGACGACUCGCCCGA 0370. _H32 1676_ H04
TCCT7CGTCTGTTCGTCCC GGGAGGACGAUGCGG UCCU UCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0371_ _H32 0046_ ,H04
A ATG GTG AAG GG AG G TACTG 6GGAGGACGAUGCGGAAUGGUGAAGGGAGG UACUGCAGACGACUCGCCCGA 0372. _H32
G CCTTTCGTTCTGCC CTCG C GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCUCGCCAGACGACUCGCCCGA 0373. .H32 0618.. _HQ4
TGTAGGAGGAGGAGGCACCG GGGAGGACGAUGCGG UGUAGGAGGAGGAGGCACCGCAGACGACUCGCCCGA 0374. JH32
ATTGGTGAA6GGAGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGUACUGCAGACGACUCGCCCGA 0375. ,H32
66ΰΤ6ΤΑ06ΰΤασΤ€€6 GGGAGGACGAUGCGG UUG6GUG UAGGGUGGUCCGCAGACGACUCGCCCGA 0376.. ..H32 2424_ .H04
CCTCATGTTTG GCTTTCGTC GGGAGGACGAUGCGGCCUCAUG UU UGGCUUUCGUCCAGACGACUCGCCCGA 0377. _H32 21Q3_ .H0
GACTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGACU UUCGUUGUGACCUCCCCAGACGACUCGCCCGA 0378. _H32 4997. .H04
TTGGGGTAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGGUAGGGUGGUACCGCAGACGACUCGCCCGA 0379. _H32 1745. _H04
TTTGGGGTTTTCCCGTGCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCG UGCCCAGACGACUCGCCCGA 0380. _H32 0446_ .H04
GCCTTTAGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUAGUUCUGCCCUCCCCAGACGACUCGCCCGA 0381. „H32 0612_ .H04
ATTGGAGAAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGAGAAGGGAGGGACUGCAGACGACUCGCCCGA 0382. _H32
TGTTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUGUUGAAGGGUGAUGGG JCCGCAGACGACUCGCCCGA 03.83. JH32 0362.. .H04
GCCTTTCGTTCTGACCTCTC GGGAGGACGAUGCGGGCCUUUCGUUCUGACCUCUCCAGACGACUCGCCCGA 0384 „H32
GCCTATCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUAUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0385. _H32 0623. . H04
TTTGAAGGGTGATGGGGTCCG GGGAGGACGAUGC6GU UUGAAGGG UGAUGGGGUCCGCAGACGACUCGCCCGA 0386. _H32 1085. .H04
CGACTCCTCTGTTCCCTCCC GGGAGGACGAUGCGGCG'ACUCCUCUGUUCCCUCCCCAGACGACUCGCCCGA 0387. _H32 2759_ H04
GCCTTTCGTTCTGACCTCCT GGGAGGACGAUGCGGGCCU UUCGUUCUGACCUCCUCAGACGACUCGCCCGA 0388. -H32
TTTGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUUUGGGUG UAGGGUGG UACCGCAGACGACUCGCCCGA 0389. _H32 0773. .H04
TGTAGGAGGAGGTGGTACCG GGGAGGACGAUGCGGUeUAGGAGGAGG UGGUACCGCAGACGACUCGCCCGA 0390. _H32
TAGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUAGGGUG UAGGGUGGUACCGCAGACGACUCGCCCGA 0391 H32 1945 H04
# in H4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc
TTGGGTGGAGGGTGGGACCG GGGAGGACGAUGCGGU'JGGG UGGAGGGUGGGACCGCAGACGACUCGCCCGA 0392_ _H32 1352. .H04
G CCTTTCGTTCCGACCTCCC GGGAGGACGAUGCGGGCCU UUCGU UCCGACCUCCCCAGACGACUCGCCCGA 0393_ H32
ATTGGTGTAGG GTG G TACTG GGGAGGACGAUGCGGAUUGGUGUAGGGUGGUACUGCAGACGACUCGCCCGA 0394. _H32
CCTTTCGTTCTG ACCTCCC GGGAGGACGAUGCGGCCUUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0395, H32 2895. _H04
ATGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGAUGGUGAAGGGAGGUACCGCAGACGA.CUCGCCCGA 0396. _H32 2852. .H04
GCCTTTCGTTCTGCC G TCCC GGGAGGACGAUGCGGGCCUUUCGU UCUGCCGUCCCCAGACGACUCGCCCGA 0397. .H32 0457. .H04
TCCCGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCCG UCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0398. .H32 0105. .H04
ATTG G TAAGGG AG G TACTG G6GAGGACGAUGCGGAUUGGUAAGGGAGGUACUGCAGACGACUCGCCCGA 0399. .H32
G ACTGTATG G G 6CTCTGTG GGGAGGACGAUGCGGGACUGUAUGGGGCUCUGUGCAGACGACUCGCCCGA 0400. .H32 2188. _H04
GCGTTTCCTCTGTTCGTCCC GGGAGGACGAUGCGGGCGU UUCCUCUGUUCG UCCCCAGACGACUCGCCCGA 0401. .H 2 0084. .H04
TTTGAAGGGTGATGGGTTG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUUGCAGACGACUCGCCCGA 0402. .H32 1744. _MQ4
ATTGGTGAAGGGAGGTCCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUCCGCAGACGACUCGCCCGA 0403_ H32
TCCTGTCGTCTGTTCGTTCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCG UUCCCAGACGACUCGCCCGA 0404 _H32 0068. H04
TTTGAAGGGCGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGCGGUGGGUCCGCAGACGACUCGCCCGA 0405. .H32 0611. _H04
TTTG AAG G G AG GTGG GTCCG GGGAGGACGAUGCGGUUUGAAGGGAGGUGGGUCCGCAGACGACUCGCCCGA 0406. _H32 0954. _H04
CAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0407. .H32
ATTGGTGCAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGCAGGGAGG UACUGCAGACGACUCGCCCGA 0408. ^H32
TGTAGGAGGAGGGGGTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGGGGUACCGCAGACGACUCGCCCGA 0409. ,H32
CAGAGGAGAAGGGAGGATGC GGGAGGACGAUGCGGCAGA6GAGAAGGGAGGAUGCCAGAC6ACUCGCCCGA 0410. H32
ATCGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGAUCGGUGAAGGGAGGUACCGCAGACGACUCGCCG A 04.11. H32 4273. „H04
TTTGGGGTTTCCCGTGCCC GGGAGGACGAUGCGGU UUGGGGUUUCCCGUGCCCCAGACGACUCGCCCGA 0412. H32 0264. .H04
TTG G GTG TCGG GTG GTACCG GGGAGGACGAUGCGGU UGGGUGUCGGGUGGUACCGCAGACGACUCGCCCGA 0413. H32 2046 .H04
GCCTTTCATTCTGACCTCCC GGGAGGACGAUGCGGGCCUUUCAUUCLfGACCUCCCCAGACGACUCGCCCGA 0414. .H32 5102. .H04
TTAGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGU UAGGGUG UAGGGUGGUACCGCAGACGACUCGCCCGA 0415. _H32
TTT6AAGG6TGGTGGTCCG GGGAGGACGAUGCGGU UUGAAGGG UGG UGGLiCCGCAGACGACUCGCCCGA 0416. ,H32 0878. H04
CCCTTTCGTTCTGACCTCCC GGGAGGACGAUGC6GCCCUU UCGUUCUGACCUCCCCAGACGACUCGCCCGA 0417. ,H32
CGATGCTTACGTGCACGCGC GGGAGGACGAUGCGGCGAUGCU UACGUGCACGCGCCAGACGACUCGCCCGA 0418. H32 0504. _H04
CAGAGGAGAAGGGAG6GTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGGUGCCAGACGACUCGCCCGA 0419 H32
# ίπ Η4
HTS read variable region RNA sequences # in H3 selection selection
ATTGGTGAAG'GGAGGTACTC G6GAGGACGAUGCGGAUUGGUGAAGGGAGGUACUCCAGACGACUCGCCCGA 0420. _H32
ATTGGTGTAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGUGUAGGGA6GUACCGCAGACGACUCGCCCGA 0421. _H32 1031_ H04
ATTGGAG.AAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGAGAAGGGAGG UACCGCAGACGACUCGCCCGA 0422. .H32 4423. H04
TTGGGAGGAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGAGGAGGGUGGUACCGCAGACGACUCGCCCGA 0423_ .H32 1963_ H04
ATCCTGGTCGTCTTACACTG GGGAGGACGAUGCGGAUCCUGGUCGUCUUACACUGCAGACGACUCGCCCGA 0424. H32
G CCTTTTGTTCTGACCTCCC GGGAGGACGAUGCGGGCCUUU UGUUCUGACCUCCCCAGACGACUCGCCCGA 0425. .H32
TTTGGAGGGGGGTGGGACCG GGGAGGACGAUGCGGUU'UGGAGGGGGGUGGGACCGCAGACGACUCGCCCGA 0426. .H32 2215. H04
GCCTTTC6TTCTGACCTAC C GGGAGGACGAUGCGGGCCUUUCGUUCUGACCUACCCAGACGACUCGCCCGA 0427, .H3.2
GCCTTCCGTTCTGACCTCCC GGGAGGACGA-UGCGGGCCUUCCGUUCUGACCUCCCCAGACGACUCGCCCGA 0428. _H32 4224_ .H04
TTTGAAGGGTGGTGGGACCG GGGAGGACGAUGCGGUUUGAAGGGUGGUGGGACCGCAGACGACUCGCCC.GA 0429. .H32 4266. .H04
AT AG GG AGAAG G GTG G ACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGUGGACCGCAGACGACUCGCCCGA 0430. ,H32
GACTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGACU UUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0431_ _H32 0699_ H04
CAGAGGAGAAGGGGGGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGGGGUUGCCAGACGACUCGCCCGA 0432_ _H32
GCCTTTC6TTCTGCCCTC GGGAGGACGAUGCGGGCCU UUCGUUCUGCCCUCCAGACGACUCGCCCGA 0433. 32 1389. .H04
TCCTGT6TCTGTTCG TGCC GGGAGGACGAUGCGGUCCUGUGUCUGUUCG'UCCCCAGACGACUCGCCCGA 0434_ H32 0153_ _H04
TATTGGAGGCGTAGGGACCG GGGAGGACGAUGCGGUAUUGGAGGCGUAGGGACCGCAGACGACUCGCCCGA 0435. ^H32 3277„ _H04
GTCTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGUCUUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0436. .H32
TGTAGGAGGAGGAGGAACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGGAACCGCAGACGACUCGCCCGA 0437. _H32
GCCTTTC6ATCTGACCTCCC GGGAGGACGAUGCGGGCCUUUCGAUCUGACCUCCCCAGACGACUCGCCCGA 0438. .H32 3555_ .H04
TGTAGGAGGGGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAGGGGGAGGUACCGCAGACGACUCGCCC A 0439. .H32
CAGTGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGUGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 044Q_ _H32
CAGGAGAAGGGAGGTGGCAT GGGAGGACGAUGCGGCAGGAGAAGGGAGGUGGCAUCAGACGACUCGCCCGA 0441. _H32
ATTGGTG ACGG GAG GTACTG GGGAG6ACGAUGCGGAUUGGUGACGGGAGGUACUGCAGACGACUCGCCCGA 0442, .H32
ATTGGTGAAGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGAGGUACUGCAGACGACUCGCCCGA 0443. .H32
G CCTTTCGTTATG AC CTCCC GGGAGGACGAUGCGGGCCUUUCGUUAUGACCUCCCCAGACGACUCGCCCGA 0444. .H3 490I_ .H04
TCCTGTCGCCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGCCUGUUCGUCCCCAGACGACUCGCCCGA 0445. _H32 0095. H04
CCCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGCCCLUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0446. .H32 0697. _H04
ATTG GTG AAG G GAG G TA ATG GGGA6GACGAUGCGGAUUGGUGAAG6GAGGUAAUGCAGACGACUCGCCCGA 0447 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CAGGGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGGGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 04 8_ _H32
GCCTTTCGTTCTGCCCGCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCGCCCCAGACGACUCGCCCGA 0449_ .H32 0696. _H04
ATTGGTGAAGGGAGGTCCTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUCCUGCAGACGACUCGCCCGA 0450_ .H32
TATTGGAGGTGTAGGGACTG GGGAGGACGAUGCGGUAU UGGAGGUG UAGGGACUGCAGACGACUCGCCCGA 0451_ H32
TTTGGGGTTTTTCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGUUU UUCCCGUGCCCCAGACGACUCGCCCGA 0452_ H32 0184. H04
TGCTGTCGTCTGTT'CGTCGC GGGAGGACGAUGCGGUGCUGUCGUCUGUUCG UCCCCAGACGACUCGCCCGA 0453. H32 0590. H04
TCCTGTCTTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCUUCUGUUCGUCCCCAGACGACUCGCCCGA 0454_ H32 0263. H04
TCCTGTCGTCTGTTCGTCCG GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCGCAGACGACUCGCCCGA 0455_ H32 0085. .H04
GCCTTTCGTTGACCTCCC GGGAGGACGAUGCGGGCCUUUCGU UGACCUCCCCAGACGACUCGCCCGA 0456. .H32
TTGGGTGTAGGGTTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUUACCGCAGACGACUCGCCCGA 0457_ .H32 2567. .H04
TTTGAAGGGTGATGCGTCCC GGGAGGACGAUGCGGU UUGAAGGGUGAUGCGUCCCCAGACGACUCGCCCGA 0458. _H32 066B. _H04
CAGAGGAGAAGGGAGGCTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGCUGCCAGACGACUCGCCCGA 0459. _H32
G CCTTTCGTTTG ACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUUGACCUCCCCAGACGACUCGCCCGA. 0460_ H32
TCCTGTC GTCTGTCG TCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUCGUCCCCAGACGACUCGCCCGA 0461. H32 0161. H04
TGTAGGGGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGGGGAGGAGGUACCGCAGACGACUCGCCCGA 0462. H32
ATTGGTGAAGG GACGTACTG GGGAGGACGAUGC6GAUUGGUGAAGGGACGUACUGCAGACGACUCGCCCGA 0463. .H32
TGTAGGAGGAGGAGGTTCCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGG UUCCGCAGACGACUCGCCCGA 0464. H32
6 CCTTTC GTTCTTACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUACCUCCCCAGACGACUCGCCCGA 0465. _H32 3433. _H04
ATTGGTAAAGGGAGGTACCG GGGAGGACGAU6CGGAUUGGUAAAGGGAGGUACCGCAGACGACUCGCCCGA 0466. .H32 4891. .H04
CAGAGGTGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGUGAAGGGAGG UUGCCAGACGACUCGCCCGA 0467. _H32
ATAGGTGTAGGGAGGTTTAG GG6AGGACGAUGCGGAUAGGUGUAGGGAGG UUUAGCAGACGACUCGCCCGA 0468. H32
GCCTTTGTTCTG CCCTCCC GGGAGGACGAUGCGGGCCUUUGUUCUGCCCUCCCCAGACGACUCGCCCGA 0469. .H32 0505. _H04
TTGGGTGTAGGGTGGTTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUUACCGCAGACGACUCGCCCGA 0470. .H32 4482. ^H04
ATTGGTG AAGG G AG TTACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGU UACCGCAGACGACUCGCCCGA 0471. _H32
TTTGAAGGGTGATGGGGT6 GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGGUGCAGACGACUC6CCCGA 0472. ,H32 1782_ _H04
GCCTTACGTTCTGCCCTCCC G6GAGGACGAUGCGGGCCUUACGUUC1JGCCCUCCCCAGACGACUCGCCCGA 0473. _H32 09S7. _H04
TTG G GTGTAGG GTG G GTC CG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGG UCCGCAGACGACUCGCCCGA 0474. „H32 2947. _HQ4
ATAGGGAGAAGGGTGATCCG GGGAGGACGAUGCGGAUAGGGAGAAGGGUGAUCCGCAGACGACUCGCCCGA 0475 H32
# ln H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
AGTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAGUGGUGAAGGGAGG UACUGCAGACGACUCGCCCGA 0476. .H32
CAGAGGAGGAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAGGAGGGAGG UUGCCAGACGACUCGCCCGA 0477. _H32
TGTAGGAGGAGGAGTTACCG GGGAGGACGAUGCGG UGUAGGAGGAGGAGUUACCGCAGACGACUCGCCCGA 0478.. -H32
TTTG AAG G GTATGG G TCCG GGGAGGACGAUGCGG UUUGAAGGGUAUGGGUCCGCAGACGACUCGCCCGA 0479. .H32 1503_ .H04
CATAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAUAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0480_ _H32
G ATCATG CTTTCCCTTCGTC GGGAGGACGAUGCGGGAUCAUGCUUUCCCUUCGUCCAGACGACUCGCCCGA 0481. _H32 0897. .H04
CAGAGGAGAAGGGTGGTTGC GGGAGGACGAUGCGGCACACGAGAAGGCUGG UUGCCAGACGACUCGCCCGA 0482. _H32
TTTG AAGG GTG GTG GGTCCC GGGAGGACGAUGCGGUUUGAAGGGUGGUGG6UCCCCAGACGAC.UCGCCCGA 0483. .H32 0829. .H04
TTCGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUUCGGUGUAGGG UGGUACCGCAGACGACUCGCCCGA 0484, _H32 2665. .H04
CGTTTCCTCTGGTTCGTCAC GGGAGGACGAU6CGGCGUUUCCUCUGGU UCG UCACCAGACGACUCGCCCGA 0485. -H32
CTG TC CTT ATTCT G C A G G T C GGGAGGACGAUGCGGCUGUCCU UAUUCUGCAGGUCCAGACGACUCGCCCGA 0486. .H32
rTGGGGTTTCCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGUUUCCCCGUGCCCCAGACGAC'JCGCCCGA 0487. .H32 0442_ _H04
GCCTTTCGTTCTGACCTCCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGACCUCCCCCAGACGACUCGCCCGA 0488. _H32
TTTGAAGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGAUGGGUCCGCAGACGACUCGCCCGA 0489. .H32 1020_ .H04
CGTAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGCGUAGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 0490. _H32
AATCGTTCTCCTGGTCTGGC GGGAGGACGAUGCGGAAUCGUUCUCCUCGUCUGGCCAGACGACUCGCCCGA 0491. _H32
GCCTTTCGTTCTCTTCTCCC GGGAGGACGAUGCGGGCCU UUCGUUCUCUUCUCCCCAGACGACUCGCCCGA 0492. _H32 0582. _H04
TTTGGAGTAGGGTGGTACCG GGGAGGACGAUGCGGUUUGGAGUAGGGUGGUACCGCA ACGACUCGCCCGA 0493. _H32 2843. _H04
AGTTGGTGGAGGTGGTTTCG GGGAGGACGAUGCGGAGUUGGUGGAGGUGGUUUCGCAGACGACUCGCCCGA 0494. .H32
GCCTTTCGTTCTGAGCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGAGCUCCCCAGACGACUCGCCCGA 0495. .H32
G CCTTTCGTTCTGACCTC GGGAGGACGAUGCGGGCCU UUCGUUCUGACCUCCAGACGACUCGCCCGA 0496. .H32 4509. .H04
G TCCTGTCGTCTG CTCGTCCC GGGAGGACGAUGCGGGUCCUGUCGUCUGCUCGUCCCCAGACGACUCGCCCGA 0497. .H32 2488. .H04
GA I GACGGCGGTTGTCTTAC GGGAGGACGAUGCGGGAUGACGGCGGUUGUCUUACCAGACGACUCGCCCGA 0498. _H32
ACTGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGACUGGUGAAGGGAGG UACCGCAGACGACUCGCCCGA 0499. .H32
TTTGCAGGGCG ATGG GTCCG GGGAGGACGAUGCGGUUUGCAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0500_ -H3.2 1334. H04
TTGGGGGTAGGGGGGTACCG GGGAGGACGAUGCGGUUGGGGGUAGGGGGGUACCGCAGACGACUCGCCC A 0501. .H32 2855. .H04
G CCTTTAGTTCTG ACCTCCC GGGAGGACGAUGCGGGCCU UUAGU UCUGACCUCCCCAGACGACUCGCCCGA 0502. _H32
GCCTTTCGGTCTGACCTCCC GGGAGGACGAUGCGGGCCU UUCGGUCUGACCUCCCCAGACGACUCGCCCGA 0503 H32
# in H
HIS read variable region A sequences U in H3 selection selection Selected sequenc
TTG GGTGTAGG GTG GTAACG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGUAACGCAGACGACUCGCCCGA 0504. H32 3440. .H04
TGTAGGAGGTGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAGGUGGAGGUACCGCAGACGACUCGCCCGA 0505, .H32
TTTG AAAAGTG ATGG GTCCG GGGAGGACGAUGCGGU UUGAAAAG UGAUGGGUCCGCAGACGACUCGCCCGA 0506. _H32 2048. .H04
GCCTTTCGTTCGGCCCTCCC GGGAGGACGAUGCGGGCCU UUCGUUCGGCCCUCCCCAGACGACUCGCCCGA 0507_ H32 0919. _H04
CAGAGGGGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGGGAAGGGAGGUUGCCAGACGACUCGCCCGA 0508. H32
TCCTTGTCGTCTTGCG CCC GGGAGGACGAUGCGGUCCU UGUCG UCUUGCGCCCCAGACGACUCGCCCGA 0509. H32
GCCTTTCGTTCTGACGTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGACGUCCCCAGACGACUCGCCCGA. 0510_ H32 3172. H04
TTTGAAGGGTGATGGGTACTG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUACUGCAGACGACUCGCCCGA 0511. H32
AGTAACCTGTTGAACTGCAC GGGAGGACGAUGCGGAGUAACCUGUUGAACUGCACCAGACGACUCGCCCGA 0512. .H32 2997. _H04
TTTGAAGGGTGGCGGGTCCG GGGAGGACGAUGCGGU UUGAA6GGUGGCGGGUCCGCAGACGACUCGCCCGA 0513. .H32 1540. „HG4
TTGGGTGTAGGGTGGTGCG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGUGCGCAGACGACUCGCCCGA 0514. .H32
TTTGGTGGAGGGTGGGACCG GGGAGGACGAUGCGGUUUGGUGGAGGGUGGGACCGCAGACGACUCGCCCGA 0515. H32
TCCTAG GTTGTCTTATTCTG GGGAGGACGAUGCGGUCCUAGGUUGUCUUAUUCUGCAGACGACUCGCCCGA 0516. .H32
GTCCTCTGGCTTATCGTCCC GGGAGGACGAUGCGGGUCCUCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 0517. H32 1092. _H04
TTTG AAG G GTG ATGG GTTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUUCCGCAGACGACUCGCCCGA 0518. .H32 0862. „H04
TTTG GAG G AG G ATGGG ACCG GGGAGGACGAUGCGGUUUGGAGGAGGAUGGGACCGCAGACGACUCGCCCGA 0519. ,H32 4706. ,H04
TTTGGGGTCTTCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGUCUUCCCGUGCCCCAGACGACUCGCCCGA 0520_ H32 Q629. _H04
TCCTGTCGTCTGTTCGCCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGCCCCCAGACGACUCGCCCGA 0521. _H32 0083. .H04
ATTGGTCAAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUCAAGGGAGGUACUGCAGACGACUCGCCCGA 0522. _H32
TTTG AAGGGTGATGG GTCTT GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCUUCAGACGACUCGCCCGA 0523. ,H32 3291. H04
CG TTTTAT ACTG CAAG TGCC GGGAGGACGAUGCGGCGUU UUAUACUGCAAGUGCCCAGACGACUCGCCCGA 0524. .H32
TTGGGTGTAGGGTGGTAC GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUACCAGACGACUCGCCCGA 0525. H32 2926. .H04
TGTGGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUGGGAGGAGGAGGUACCGCAGACGACLICGCCCGA 0526. _H32
TTGGGGGTAGGGCGGTACCG GGGAGGACGAUGCGGUUG6GG6UAGGGCGGUACCGCAGACGACUCGCCCGA 0527. H32 2723. .H04
TTTG G AGTAG G G TG G G ACCG GGGAGGACGAUGCGGUUUGGAGUAGGGUGGGACCGCAGACGACUCGCCCGA 0528. H32 3177. _H04
TCCTGTCGTCTGTITGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUUGUCCCCAGACGACUCGCCCGA 0529. H32 0103. _H04
TTCGAAGGGTGGTGGG TCCG GGGAGGACGAUGCGGUUCGAAGGGUGGUGGGUCCGCAGACGACLJCGCCCGA 0530. _H32 2128. _H04
TTGGGAGTAGGGCGGTACCG GGGAGGACGAUGCGGUUGGGAGUAGGGCGGUACCGCAGACGACUCGCCCGA 0531 H32 2.928 H04
# ϊη Η4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTGGAGGGTGATGGGACCG GGGAGGACGAUGCGGUUUGGAGGGUGAUGGGACCGCAGACGACUCGCCCGA 0532. .H32 2912... H04
TCCTGCCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGCCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0533. .H32 0136. _H04
Ml&GTGAAAGG GGTACCG GGGAGGACGAUGCGGAUUGGUGAAAGGAGGUACCGCAGACGACUCGCCCGA 0534. H32
TGACTCGTCTGTTTTCGTCCC GGGAGGACGAUGCGGUGACUCG UCUGUUUUCGUCCCCAGACGACUCGCCCGA 0535. _H32 3151. _H04
TTTG AAG G AGG ATG G G ACCG GGGAGGACGAUGCGGUUUGAAGGAGGAUGGGACCGCAGACGACUCGCCCGA 0536_ H32 3008. H04
1TG CGTGTAG G GTGG TACCG GGGAGGACGAUGCGGUUGCGUGUAGGGUGG UACCGCAGACGACUCGCCCGA 0537. .H32 4024. _H04
TTGGGTGTAGCGTGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGCGUGGUACCGCAGACGACUCGCCCGA 0538. .H32 3144. _H04
TTTGAAGGGGGATGGGGCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGGCCGCAGACGACUCGCCCGA 0539. ^H32 1221. „H04
CTAAGAAAGGGAGGTGGTGC GGGAGGACGAUGCGGCUAAGAAAGGGAGGUGG UGCCAGACGACUCGCCCGA 0540. _H32
TTCTGTCGTCTG TTCGTCCC GGGAGGACGAUGCGGUUCUGUCGUCUGU UCGUCCCCAGACGACUCGCCCGA 0541_ .H32 0250. _H04
TCCTGTCGTCTTTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUUUUCGUCCCCAGACGACUCGCCCGA 0542_ _H32 0063. H04
TGGAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGGAGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 0543. H32
TGTAGAAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGAAG6AGGAGGUACCGCAGACGACUCGCCCGA
as 0544. H32
TATTGGAGGAGTAGGGACCG GGGAGGACGAUGCGGUAUU6GA6GAGUAGGGACCGCAGACGACUCGCCC6A 0545. .H32 4722. _H04
AAACGAGC CGTCTTATCCTG GGGAGGACGAUGCGGAAACGAGCCGUCU UAUCCUGCAGACGACUCGCCCGA 0546. .H32
TTTGGGGCTTTCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGCUUUCCCGUGCCCCAGACGACUCGCCCGA 0547. .H32 1260. _HQ4
TCCTG TC GTCTG TTCG TTTC GGGAGGACGAUGCGGUCCUG UCGUCUGUUCGUUUCCAGACGACUCGCCCGA 0548. _H32 0207. _HG4
CGTTTCATCTGG i'TCGTCCC GGGAGGACGAUGCGGCG UUUCAUCUGGUUCGUCCCCAGACGACUCGCCCGA 0549. _H32
GATGAAGGCCTGTCGTGTAC GGGAGGACGAUGCGGGAUGAAGGCCUGUCGUG UACCAGACGACUCGCCCGA 0550. .H32
CAATAGGAGGAGTGGGGCGC GGGAGGACGAUGCGGCAAUAGGAGGAGUGGGGCGCCAGACGACUCGCCCGA 0551. .K32
ATT6 GTGAAGGCAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGCAGGUACUGCAGACGACUC6CCCGA 0552. .H32
TGTTGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUGUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 0553. H32
CTTGAA6GGTGGTGGGTCCG GGGAGGACGAUGCGGCU UGAAGGGUGGUGGGUCCGCAGACGACUCGCCCGA 0554, _ 32 2043. _H04
TTTGAAGGGAGCTGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGAGCUGGGUCCGCAGACGACUCGCCCGA 0555. H32 1319. _H04
GCCmCGTTGTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCG UUGUGCCCUCCCCAGACGACUCGCCCGA 0556. ,H32 0932. _H04
GCCTTTCGTTCAGACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCAGACCUCCCCAGACGACUCG CCCGA 0557. ,H32
GCCTTTCGTTCTGACCTTTC GGGAGGACGAUGCGGGCCUUUCG UUCUGACCUUUCCAGACGACUCGCCCGA 0558. _H32
GCCTTTCGTTTTGACCTCCC GGGAGGACGAUGCGGGCCUUUCG UUUUGACCUCCCCAGACGACUCGCCCGA 0559 H32
# in H4
HTS read variable region NA sequences tt in H3 selection selection Selected sequenc
ATTGGGGAAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGGGAAGGGAGGUACCGCAGACGACUCGCCCGA 0560. „H32
TGCAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGCAGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 0561. „H32
GCCTTTGGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCU UUGGUUCUGCCCUCCCCAGACGACUCGCCCGA 0562_ .H32 1821. „H04
AT1"GGGAAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGGAAGGGAGGUACUGCAGACGACUCGCCCGA 0563. .H32
ATAGGGAGGAGGGTGAACCG GGGAGGACGAUGCGGAUAGGGAGGAGGGUGAACCGCAGACGACUCGCCCGA 0564_ H32
TTTGAAGGGTGATAGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGUGAUAGGG UCCGCAGACGACUCGCCCGA 0565, ,H32 0587. .H04
TTG GGTTAG GGTGGTACCG GGGAGGACGAUGCGGU UGGGUUAGGGUGGUACCGCAGACGACUCGCCCGA 0566_ .H32 2099, „H04
TTTGAAGG6CGCTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGCGCUGGGUCCGCAGACGACUCGCCCGA 0567.. .H32 1173. „H04
CTTGAAGGGAGATGGGTCCG GGGAGGACGAUGCGGCUUGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 0568. _H32 2106. .H04
TTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGU UGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0569. .H32
TTTGAAGGGAGATGGGACCG GGGAGGACGAUGCGGU UUGAAGGGAGAUGGGACCGCAGACGACUCGCCCGA 0570_ .H32 1693. _H04
GCC7TTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCU UUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0571. .H32
ATTCGTGMG G G AG GTACTG GGGAGGACGAUGCGGAUUCGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0572. -H32
TTGGGTGTAGGGTCGTACCG GGGAGGACGAUGCGGU UGGGUGUAGGGUCGUACCGCAGACGACUCGCCCGA 0573.. .H32
TGAAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGAAGGAGGAGGAGG UACCGCAGACGACUCGCCCGA 0574_ _H32
TTTTTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGU UUUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0575. ,H32 1425. _H04
CATC G GTTCTC ATCG TTG TG GGGAGGACGAUGCGGCAUCGGU UCUCAUCGUUGUGCAGACGACUCGCCCGA 0576. _H32
CTGAGGAGAAGGGAGGTTGC GGGA6GACGAUGCGGCUGAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0577. _H32
TCCTGTCGTCTGTTCGTC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCAGACGACUCGCCCGA 0578. .H32 0212. _H04
TGTAGGAGGAGAAGGTACCG GGGAGGACGAU6CGGUGUAGGAGGAGAAGG UACCGCAGACGACUCGCCCGA 0579. H32
ATGGGTGAAGGGAGGTACCG GGGA6GA\CGAUGCGGAUGGGUGAAGGGAGG UACCGCAGACGACUC6CCCGA 0580. _H32
TCCTGTCGTCTGTTCATCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCAUCCCCAGACGACUCGCCCGA 0581. H32 0111. .H04
GCCTTTCGTTCTCACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUCACCUCCCCAGACGACUCGCCCGA 0582. .H32 5106. .H04
TTTGAAGGGCGATGGGCCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGCCCGCAGACGACUCGCCCGA 0583. _H32 1332. _H04
ATTGGTGAAGAGAG6TACCG GGGAGGACGAUGCGGAUUGGUGAAGAGAGGUACCGCAGACGACUCGCCCGA 0584. H32
TTGGAAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUGGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0585. ,H32 2232. _H04
TTTGGA6GGGGGTGGGTCCG GGGAGGACGAUGCGGUUUGGAGGGGGGUGGGUCCGCAGACGACUCGCCCGA 0586. _H32
TTG G G CGTAGG GCG GTACCG GGGAGGACGAUGCGGUUGGGCGUAGGGCGGUACCGCAGACGACUCGCCCGA 0587. _H32 2364. _H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTG AAG G GAG ATG G G CCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGGGCCCGCAGACGACUCGCCCGA 0588_ H32 1610. ,H04
TTTGAAGGGTGGAG GTCCG GGGAGGACGAUGCGGUUUGAAGGGUGGAGGGUCCGCAGACGACUCGCCCGA 0589. H32 2249. _HQ4
TTTGCA6GGAGATGGGTCCG GGGAGGACGAUGCGGUUUGCAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 0590. H32 1795. H04
TAGAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGUAGAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 059I_ .H32
GCCTTTCGTTCTGCCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCCCCAGACGACUCGCCCGA 0592. .H32 0909. .H04
GTGTTCGTCTGTTTTCGTCCC GGGAGGACGAUGCGGGUGUUCGUCUGUU UUCGUCCCCAGACGACUCGCCCGA 0593. .H32
TTTGAAGGGTGATGGGTACCT GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUACCUCAGACGACUCGCCCGA 0594. _H32
CAGAGGAGAGGGGA6GTTGC GGGAGGACGAUGCGGCAGAGGAGAGGGGAGGUUGCCAGACGACUCGCCCGA 0595_ .H32
GCCTTTCGTTCTGACCACCC GGGAGGACGAUGCGGGCaj UUCGUUCUGACCACCCCAGACGACUCGCCCGA 0596. .H32 4763. .H04
ATTAG TG AAGG 6 AG GTACCG GGGAGGACGAUGCGGAUUAGUGAAGGGAGG UACCGCAGACGACUCGCCCGA 0597. H32
GC CTTTGTTCTG ACCTCCC GGGAGGACGAUGCGGGCCU UUGUUCUGACCUCCCCAGACGACUCGCCCGA 0598. H32 7Q1_ .H04
GTAGGGAGAAGGGTGAACCG GGGAGGACGAUGCGGGUAGGGAGAAGGG UGAACCGCAGACGACUCGCCCGA 0599. .H32
TATTGGGGGTGTAGGGACCG GGGAGGACGAUGCGGUAUUGGGGGUGUAGGGACCGCAGACGACUCGCCCGA 0600_ H32 4972. _H04
ATTGGTGAAGGGAGGTAGTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUAG UGCAGACGACUCGCCCGA 0601. H32
TTGGGAGTAG6GAGGTACCG GGGAGGACGAUGCGGU UGGGAGUAGGGAGGUACCGCAGACGACUCGCCCGA 0602. _H32 3743. _H04
TCCTGTCGTCTTGCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUUGCGUCCCCAGACGACUCGCCCGA 0603. „H32 0162. .H04
ATAGG 6AGG AGGTGAATGTG GGGAGGACGAUGCGGAUAGGGAGGAGGUGAAUGUGCAGACGACUCGCCCGA 0604. H32
TTGGGTGTAGGGTGAACCG GGGAGGACGAUGCGGU UGGGUGUAGGG UGAACCGCAGACGACUCGCCCGA 0605. H32
TGTTGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUUGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 0506. .H32
GCCTTTCTTTCTGACCTCCC GGGAGGACGAUGCGGGCCU UUCUUUCUGACCUCCCCAGACGACUCGCCCGA 0607. .H32 4429. .H04
ATTG G TG A AG CG AG G T A CTG GGGAGGACGAUGCGGAUUGGUGAAGCGA6GUACUGCAGACGACUCGCCCGA 0608. _H32
TTTGAAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUU6AAAGG6UGAUGGGUCCGCAGACGACUCGCCCGA 0609. H32 1508. .H04
GATGACGGCGGTGTCGCTAC GGGAGGACGAUGCGGGAUGACGGCGGUGUCGCUACCAGACGACUCGCCCGA 0610. .H32
GCCGTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCGUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0611. H32 1179. _H04
ATTGCTGAAGGGAGGTACTG GGGAGGACGAUGCGGAUUGCUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0612. .H32
TTGGGTGTAGGGCGTTACC6 GGGAGGACGAUGCGGUUGGGUGUAGGGCGUUACCGCAGACGACUCGCCCGA 0613. _H32 4176. .H04
TTTGAAGGGGGATG6GCCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGCCCGCAGACGACUCGCCCGA 0624. ..H32 2009. .H04
TTTG ACGGG CG ATGG GTCCG GGGAGGACGAUGCGGUUUGACGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0615. .H32 1481. _H04
# in H4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc
TTTG ACG G G AGATQ GGTCCG GGGAGGACGAUGCGGU UUGACGGGAGAUGGGUCCGCAGACGACUCGCCCGA 0616_ ,H32 IS 94, _H0A
TCCTATCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUAUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0617_ „H32 0113_ _HQ4
G CGTTTCG TTCTGCCCTCCC GGGAGGACGAUGCGGGCGUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 06 8_ 32 13D3_ .H04
TTTGAGGTGATGGGTCCG GGGAGGACGAUGCGGU UUGAGGUGAUGGGUCCGCAGACGACUCGCCCGA 0619_ H32 2938. H04
iTTGAAGGGTGAGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAGGG UCCGCAGACGACUCGCCCGA Q620_ H32 2271. .H04
TTTG AAG G GGG ATG GGTCTG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGUCUGCAGACGACUCGCCCGA 0621_ H32 3783. .H04
TTTGAAGGGGGATGGTTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGG UUCCGCAGACGACUCGCCCGA 0622„ H32 2458. _H04
TTTG AAG G GGGTTG G GTCCG GGGAGGACGAUGCGGUUUGAAGGGGGU UGGGUCCGCAGACGACUCGCCCGA 0623_ H32 0856. _H04
AGTTGGAGGAGGGAGGTCTG GGGAGGACGAU6CGGAGUUSGAGGAGGGAG6UCUGCAGACGACUCGCCCGA 0624_ .H32
TCCTGTCGTCTGTTCGTCCA GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCCACAGACGACUCGCCCGA Q625_ .H32 0206. _HG4
ATTG G TG ATG G G AG G TAG C G GGGAGGACGAUGCGGAUUGGUGAUGGGAGG UACCGCAGACGACUCGCCCGA 0626. ,H32
TTTGGAGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGGAGGGUGGUGGGUCCGCAGACGACUCGCCCGA 0627_ _H32 2446. _H04
TTTGGTGTAGGGTGGGACCG GGGAGGACGAUGCGGUUUGGUGUAGGGUGGGACCGCAGACGACUCGCCCGA 0628_ .H32 3158. .H04
TCCTGTCGTCTG TTCG TCTC GGGAGGACGAUGCGGUCCUG UCGUCUGUUCGUCUCCAGACGACUCGCCCGA 0629.. .H32 0213. .H04
CGACTATGTCGTCTTGCCCC GGGAGGACGAUGCGGCGACUAUGUCGUCUUGCCCCCAGACGACUCGCCCGA Q630. .H32
CGGAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCGGAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0631. H32
GCCTTTCGTTCTG AACTCCC GGGAGGACGAUGCGGGCCU U UCGUUCUGAACUCCCCAGACGACUCGCCCGA 0632. _H32
TTTG GGGTTTTCCCTG CCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCUGCCCCAGACGACUCGCCCGA 0533. .H32 2562. _H04
ATTGATGAAGGGAGGTACCG GGGAGGACGAUGCGGAUUGAUGAAGGGAGGUACC6CAGACGACUCGCCCGA 0634. H32
TTGGGCGTAGGGAGGTACCG GGGAGGACGAUGCGGU UGGGC6UAGGGAGGUACCGCAGACGACUCGCCCGA 0635. _H32 2972_ _H04
TTTTG AAG G GTGATG GGTCCC GGGAGGACGAUGCGGU UUUGAAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 0636. .H32 1412. _H04
rrGGGAGGGGGGTGGGACCG GGGAGGACGAUGCGGU UGGGAGGGGGG UGGGACCGCAGACGACUCGCCCGA 0637. _H32 3707, .H04
TTTGAAGGGAGATGGGGCCG GGGA6GACGAUGCGGUUUGAAGGGAGAUGGGGCCGCAGACGACUCGCCCGA 0638. _H32 1562.. .H04
TTTGAAGTCGTCTTATCCTG GGGAGGACGAUGCGGUUUGAAGUCGUCUUAUCCUGCAGACGACljCGCCCGA 0639_ .H32
GCCTTTCGTACTGCGCTCCC GGGAG6ACGAUGCGGGCCUUUCGUACUGCCCUCCCCAGACGACUCGCCCGA 0640. .H32 1267. _H04
TGACTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUGACUCG UCUGUUCGUCCCCAGACGACUCGCCCGA 0641_ .H32
ATTGGTGAAGGAAGGTACCG GGGAGGACGAUGCGGAUUGGUGAAGGAAGGUACCGCAGACGACUCGCCCGA 0642. H32
TCCTGTCGTTTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUUUGUUCG UCCCCAGACGACUCGCCCGA 0543 H32 0174 H04
U in H4
HTS read variable region RNA sequences # in H3 selection selection
GCCTTTCGTCTACCCTCCC GGGAGGACGAUGCGGGCCUUUCGUCUACCCUCCCCAGACGACUCGCCCGA 0644. _H32
CGGAAAGGTGGAGGTGGTG GG6AGGACGAUGCGGC6GAAAGGUGGAGGUGGUGCAGACGACUCGCCCGA 0645. -H32
TTTG GTG G AGG GTG GTACCG GGGAGGACGAUGCGGUUUGGUGGAGGGUGGUACCGCAGACGACUCGCCCGA 0646_ .H32
TTTG A AG AATG ATGG 6TCCG GGGAGGACGAUGCGGUUUGAAGAAUGAUGGGUCCGCAGACGACUCGCCCGA 0647. .H32 1972. _H04
TTTG AAGG AGG GTG G GACCG GGGAGGACGAUGCGGUUUGAAGGAGGGUGGGACCGCAGACGACUCGCCCGA 0648. ,H32 4020. .H04
ATTGGTGAAGGGTGGGACTG GGGAGGACGAUGCGGAUUGGUGAAGGGUGGGACUGCAGACGACUCGCCCGA 0649. _H32
GTTGAAGGGTGGTGGGTCCG GGGAGGACGAUGCGGGUUGAAGGGUG6UGGGUCCGCAGACGACUCGCCCGA 0650. ^H32 1480^ H04
CTTTTTACTC i T VT TTICCG GGGAGGACGAUGCGGCUUU UUACUCUUUUU UUCCGCAGACGACUCGCCCGA G6S1. .H32
CAGAG GAG AAGG AAG GTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGAAGGUUGCCAGACGACUCGCCCGA G652. _H32
GCCTTTCTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCUUCUGCCCUCCCCAGACGACUCGCCCGA 0653_ .H32 1043. j-104
CAGAGGAGAAGGGAGGTTGT GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUUG UCAGACGACUCGCCCGA QS54. . H32
GCCTTTCGTTCTGCCCTTC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCU UCCAGACGACUCGCCCGA 0655. _H32
TTAG G GTGTAGG GTG GTCCG GGGAGGAC6AUGCGGU UAGGGUGUAG66U6GUCCGCAGACGACUCGCCCGA 06S6_ .H32
TGTTTCG CTCTCCCCG TCTG GGGAGGAC6AUGCGGUGUUUCGCUCUCCCCGUCUGCAGACGACUCGCCCGA 0657. _H32
TGACTCGTCTGTCTCGTCCC GGGAGGACGAUGCGGUGACUCG UCUGUCUCGUCCCCAGACGACUCGCCCGA 0658. _H32 3518. _H04
TATTGGAGGT6TG GGGACCG GGGAGGACGAUGCGGUAUUGGAGGUGUGGGGACCGCAGACGACUCGCCCGA 0659., „H32
CG AAG GGTG ATGG GTCCG GGGAGGACGALiGCGGCGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0660. ,H32 1353. _H04
TTGAAGGGTGGTGGGTCCG GGGAGGACGAUGCGGU UGAAGGGUGGLfGGGUCCGCAGACGACUCGCCCGA 0661. .H32 2402, _HQ4
TTTAGG GTGATG G GTCCG GGGAGGACGAUGCGGUUUAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0662. _H32 2696. .H04
TTGGGTGTAGGGGGTACCG GGGAGGACGAUGCGGU UGGGU6UAGGGGG UACCGCA6ACGACUCGCCCGA 0663. _H32 3804. _H04
TTTGAAGGAGGATGGGTCCG GGGAGGACGAUGCGGU UUGAAGGAG6AUGGGUCCGCAGACGACUCGCCCGA 0664. H32 2355. _H04
TTTGAAGGGTGGGGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGUGGGGGGUCCGCAGACGACUCGCCCGA 0665. _H32 2332. H04
TGTTTG AAG GGTG ATGG GTCCG GGGAGGACGAUGCGGUGUU UGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0666. ,H32 3742. _H04
GTTGAAGGGG6ATGGGTCCG GGGAGGACGAUGCGGGUUGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0667„ .H32 1541. _H04
CAGAGGAGTAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAGUAGGGAGGUUGCCAGACGACUCGCCCGA 0668. .H32
AGGCGGCTCGTCTTATACTG GGGAGGACGAUGCGGAGGCGGCUCGUCUUAUACUGCAGACGACUCGCCCGA 0669. _H32
CTTG TCGTCTTG CG TC CC GGGAGGACGAUGCGGCU UGUCG UCUUGCGUCCCCAGACGACUCGCCCGA 0670. _H32
GTCCTGTCGTCTGTTCGTCCT GGGAGGACGAUGCGGGUCCUGUCGUCUGUUCGUCCUCAGACGACUCGCCCGA 0671. _H32 2077. _H04
# ιη H4
HTS read variable region RNA sequences S in H3 selection selection Selected sequenc
TTTG AG GGGTGGTGG GTCCG GGGAGGACGAUGCGGU UU6AGGGGUGGU6GGUCCGCAGACGACUCGCCCGA 0672_ H32 2475. _H04
ATTGGTGAAGTGAGGTACCG GGGAGGACGAUGCGGAUUGGUGAAGUGAGGUACCGCAGACGACUCGCCCGA 0673_ ,H32
TCTG A AGG GTG GTG G GTCCG GGGAGGACGAUGCGGUCUGAA6GG UGGUGGGUCCGCAGACGACUCGCCCGA 0674. H32 3166. .H04
TATTGGAGGGGTAGGGACCG GGGAGGACGAUGCGGUAUUGGAGGGGUAGGGACCGCAGACGACUCGCCCGA 0675_ H32
GATGACGGCGAGTGTGCAC GGGAGGACGAUGCGGGAUGACGGCGAGUGUGCACCAGACGACUCGCCCGA 0676. .H32
GCTCTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCUCUUCGU UCUGCCCUCCCCAGACGACUCGCCCGA 0677. H32 0280. ,H04
TTG GG GTTTTCCCGT6CCC GGGAGGACGAUGCGGU UGGGGUU UUCCCGUGCCCCAGACGACUCGCCCGA 0678. _H32 0616,. .H04
6CCTATCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCUAUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0679, H32
GGTAGGGATGGAGGTGT't'CG GGGAGGACGAUGCGGGGUAGGGAUGGAGGUGUUCGCAGACGACUCGCCCGA 0680_ .H32
ATTGGTGGAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGUGGAGGGAGGGACUGCAGACGACUCGCCCGA 0681_ .H32
TTTGGTGAAGGGAGGGACTG GGGAGGACGAUGCGGUUUGGUGAAGGGAGGGACUGCAGACGACUCGCCCGA 0682. H32
ATTGGTGAGGGAGGTACTG GG6AGGACGAUGCGGAUUGGUGAG6GAGGUACUGCAGACGACUCGCCCGA 06S3_ H32
ATAGGGAGAAGGGGGAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGGGAACCGCAGACGACUCGCCCGA 0684_ .H32
TATTG AAGG GTG ATG G GTCCG GGGAGGACGAUGCGGUAUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 0685_ .H32 1582. .H04
TTTG GAGG GCG ATG G GTCCG GSGAGGACGAUGCGGUUUGGAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0686_ .H32 1372. _HG4
GCCTTTCGTTCTGACCTCGC GGGAGGACGAUGCGGGCCUUUCGUUCUGACCUCGCCAGACGACUCGCCCGA 0687. „H32
TTTGGGG ! l l l CCCGTGTCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCGUG UCCCAGACGACUCGCCCGA 0688. H32 1943, Ή04
TCCTGTCGTCTGTCCGTCCC 6G6AGGACGAUGCGGUCCUGUCGUCUGUCCGUCCCCAGACGACUCGCCCGA 0689. ^H32 0170. _H04
TCTTTCCTGTCGTACTGGTG GGGAGGACGAUGCGGUCUUUCCUG UCGUACUGGUGCAGACGACUCGCCCGA 0690. _H32
CCTCCAGACCTGTTTCTGTC GGGAGGACGAUGCGGCCUCCAGACCUGU UUCUGUCCAGACGACUCGCCCGA 0691. _H32 2998. „H04
TG AAAG TTCTCGTCG GCCCC GGGAGGACGAUGCGGUGAAAG UUCUCGUCGGCCCCCAGACGACUCGCCCGA 0692. ,H32 2181. „H04
GCCTGTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUG UCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0693. .H32 2142. ,HQ4
TTTGAAGGGTGGTGTGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGGUGUGUCCGCAGACGACUCGCCCGA 0694. .H32 4023. .H04
TTTGAAGGGTAGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUAGUGGGUCCGCAGACGACUCGCCCGA 0695_ _M32 2851. .H04
TTGGGTGTAGGGGGTTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGUUACCGCAGACGACUCGCCCGA 0696. _H32
TTTG A AG G G TG TG G G TCCG GGGAGGACGAUGCGGUUUGAAGGGUGUGGGUCCGCAGACGACUCGCCCGA 0697. .H32 1293. _H04
1TTGAAGGTGGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGUGGAUGGGUCCGCAGACGACUCGCCCGA 0698. _H32 2392. .H04
GACAGTCGTCACTTC CTG CC GGGAGGACGAUGCGGGACAGUCGUCACUUCCUGCCCAGACGACUCGCCCGA 0699 H32
HTS read variable region R A sequences in H3 selection Selected sequenc
TTTGGGGTTTTCCTGTGCCC GGGAGGACGAUGCGGUUUGGGGUUUUCCUGUGCCCCAGACGACUCGCCCGA 07OO„H32
ATTGGTGA AG G G AGGTACCA GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACCACAGACGACUCGCCCGA 0701_H32
ACCTCCTCGAGTTGTGTCGC GGGAGGACGAUGCGGACCUCCUCGAGUUG UGUCGCCAGACGACUCGCCCGA 07O2_H32
TGGACGACCGTCTGTATGTG GGGAGGACGAUGCGGUGGACGACCGUCUGUAUGUGCAGACGACUCGCCCGA Q703_H32
GTCCTGTCGTCTGTTCGTCC GGGAGGACGAUGCGGGUCCUGUCG UCUGUUCGUCCCAGACGACUCGCCCGA 0704_H32 3037 H04
TCTCCTGTGGCGTTGTGCTG GGGAGGACGAUGCGGUCUCCUGUGGCGUUGUGCUGCAGACGACUCGCCCGA 0705 JH32
TTGGGTGTAGGGGGGCACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGGCACCGCAGACGACUCGCCCGA 0706_H3.2
GTTTG AAG GG TG ATG G GTCCG GGGAGGACGAUGCGGGUUUGAAGGGUGAUGGGUCCG'CAGACGACUCGCCCGA 0707_H32 1624„H04
TTTG AAGG 6CG ATGGG ACCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGACCGCAGACGACUCGCCCGA 070S_H32 1843„H04
TTTGATGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAUGGGGGAUGGGUCCGCAGACGACUCGCCCGA 07Q9JH32 2236_H04
CAGAGGAGATGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAGAUGGGAGG UUGCCAGACGACUCGCCCGA 0 10_H32
ATAGGGAGAAGGGTGTACCG GGGAGGAC6AUGCGGAUAGGGAGAAGGGUGUACCGCAGACGACUCGCCCGA 0711_H3'2
TGACTCGTTTGTATCTTCCC GGGAGGACGAUGCGGUGACUCGUUUGUAUCUUCCCCAGACGACUCGCCCGA 0712. H32
TAATCGTTTCCTCCTGTGTC GGGAGGACGAUGCGGUAAUCGUUUCCUCCUG UGUCCAGACGACUCGCCCGA 0713_ H32
TGTAGGAGGAGGAAGTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAAG UACCGCAGACGACUCGCCCGA 0714_H32
AGTG G GAG AAGG GTGTTACG GGGAGGACGAUGCGGAGUGGGAGAAGGG'JGUUACGCAGACGACUCGCCCGA 0715_H32
GTTGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGGUUGGGUGUAGGGUGG UACCGCAGACGACUCGCCCGA 0716_H32 3795_H04
TTTG AG GGTGATG A ATCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGAAUCCGCAGACGACUCGCXCGA 0717_ H32 2378_H04
GCCTITCGTTCTGCTTTCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGCUUUCCCCAGACGACUCGCCCGA Q71S_H32 0958_HQ4
TTG6GGGIAGGGAGGTACCG GGGAGGACGAUGCGGUUGGGGGUAGGGAGGUACCGCAGACGACUCGCCCGA 0719_H32 4463_K04
TTTGGAGGAGGGTGGTACCG GGGAGGACGAUGCGGUUUGGAGGAGGGUGGUACCGCAGACGACUCGCCCGA 0720_H32 4539_H04
CAAAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAAAGGAGAAGGGAGGUUGCCAGACGACUCGCCCGA Q721_H32
CAGAGGAGAAGAGAG GTTGC GGGAGGACGAUGCGGCAGAGGAGAAGAGAGGUUGCCAGACGACUCGCCCGA 0722_H32
TGGGTG AAG G6TGTATAG CG GGGAGGACGAUGCGG UGGGUGAAGGGUGUAUAGCGCAGACGACUCGCCCGA 0723_H32
T7TGAAG6GTGGTGGTACCG GGGAG6ACGAUGCGGU UUGAAGGGUGGUGGUACCGCAGACGACUCGCCCGA Q724_H32
TCATCTTT CTCCCATGTG GC GGGAGGACGAUGCGGUCAUCUUUCUCCCAUGUGGCCAGACGACUCGCCCGA 0725_H32
TCGATCGTTTTCCGCGTGTG GGGAGGACGAUGCGGUCGAUCG UU UUCCGCGUGUGCAGACGACUCGCr.CGA 0726_H32
G CCTTTC GTTCTGCCCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UCUGCCCCUCCCCAGACGACUCGCCCGA Q727 H32 1824 H04
# in H4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc
TCCTQACGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGACGUCUGUUCGUCCCCAGACGACUCGCCCGA Q728. _H32 0169. _H04
TTGGGTGTAGGGCGGTACTG GGGAGGACGAUGCGGU UGGG UGUAGGGCGGUACUGCAGACGACUCGCCCGA 0729. _H32
TTGGGAGGGGG ATGG GACCG GGGAGCACGAUGCGGUUGGGAGGGGGAUGGGACCGCAGACGACUCGCCCGA 0730. _H32
TTGGGTGTAGGGCGGCACCG GGGAGGACGAUGCGGUUGGG UGUAGGGCGGCACCGCAGACGACUCGCCCGA 0731. _H32 4698_ .HQ4
TTTGAAGGGAGTTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGAGUUGGGUCCGCAGACGACUCGCCCGA 0732. .H32 1770. _H04
TTG G AAGG G G GATGG GACCG GGGAGGACGAUGCGGUUGGAAGGGGGAUGGGACCGCAGACGACUCGCCCGA 0733. .H32 3353. .H04
TTTG CAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGCAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0734. _H32 2208. .H04
TATGGAGGTGT AG G GACCG GGGAGGACGAUGCGGUAUGGAGG UGUAGGGACCGCAGACGACUCGCCCGA 0735. _H32 5005. H04
TTT6TAGG GC6 ATG G GTCCG GGGAGGACGAUGCGGUUUGUAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0736. _H32 2354. ,H04
GCCGTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCGUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0737. .H32
TTTGG G GTTTTCCCCTGCCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCCUGCCCCAGACGACUCGCCCGA 0738. _H32 1629. „H04
TGTAGGAGGAGGAGGTACCC GGGAGGACGAUGCGGUGUAGGAGGAGGAGG UACCCCAGACGACUCGCCCGA 0739. .H32
TG ACTCGπTGTTTCGTCCC GGGAGGACGAUGCGGUGACUCGUUUGUUUCGUCCCCAGACGACUCGCCCGA 0740. .H32
TGTGGGTCTGGTTGTGGC GGGAGGACGAUGCGGUGUGGGUCUGGUUGUGGCCAGACGACUCGCCCGA 0741. _H32
GCCTTTCGTTCTGCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCUCCCCAGACGACUCGCCCGA 0742. _H32 0805_ _H04
GCCTTTCGTTCTGACCTGCC GGGAGGACGAUGCGGGCCUUUCGU UCUGACCUGCCCAGACGACUCGCCCGA 0743. H32 4747. _H04
TTTG AAGG ATG TGG GTCCG GGGAGGACGAUGCGGUUUGAAGGAUGG UGGGUCCGCAGACGACUCGCCCGA 0744. H32 3831. .H04
GTTGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGGUUGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0745. _H32 1018. _H04
GCCTTTCCTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCCUUCUGCCCUCCCCAGACGACUCGCCCGA 0746. .H32 1466. H04
TG TAG G A G G AG G A GG TACCA GGGAGGACGAUGCGGUGUAGGAGGAGGAGG UACCACAGACGACUCGCCCGA 0747. .H32
TTGGGAGGAGGATGGGACCG GGGAGGACGAUGCGGUUGGGAGGAGGAUGGGACCGCAGACGACUCGCCCGA 0748. _H32 3860. _H04
TAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUAGGAGGAGGAGG UACCGCAGACGACUCGCCCGA 0749. _H32
TTTG AAGG GCGATTG GTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUUGGUCCGCAGACGACUCGCCCGA 0750. _H32 1874. _H04
TTTGAAGGGCGATGGGGCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGGCCGCAGACGACUCGCCCGA 0751. _H32 1699. _H04
CAGAG AAGAAGG G AG GTTGC GGGAGGACGAUGCGGCAGAGAAGAAGGGAGG UUGCCAGACGACUCGCCCGA 0752. _H32
AGTAGGTGAAGGGTGGCCCG GGGAGGACGAUGC.GGAGUAGG UGAAGGGUGGCCCGCAGACGACUC6CCCGA 0753. „H3
CAGAGGAGAAGTGAGGTTGC GGGAGGACGAUGCGGCAGAG6A6AAGUGAGGUUGCCAGACGACUCGCCCGA 0754. .H32
CAGAGGAGAATGGAGGTTGC G6GAGGACGAUGCGGCA6AGGAGAAUGGAGG UUGCCAGACGACUCGCCCGA 0755 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CTACTTTATCCTC CAAGGGC GGGAGGACGAUGCGGCUACUUUAUCCUGCAAGGGCCAGACGACUCGCCCGA 0756, _H32
G CGCATCG TTTTGACTG CTG GGGAGGACGAUGCGGGCGCAUCGUUUUGACUGCUGCAGACGACUCGCCCGA 0757. H32
TTG GGTGTAGGGTG TTCCG GGGAGGACGAUGCGGUUGGG UGUAGGGUGUUCCGCAGACGACUCGCCCGA 0758. _H32 0354. .HQ4
CTTGGGGTTTTCCCGTGCCC GGGAGGACGAUGCGGCUUGGGGUUUUCCCGUGCCCCAGACGACUCGCCCGA 0759. .H32 0836. _HQ4
ATTG GTG AATG G AGGTACCG GGGAGGACGAUGCGGAUUGGUGAAUGGAGGUACCGCAGACGACUCGCCCGA 0760. _H32
GCCATTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCAUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0761. .H32
TCCTGTCATCTGTTCGTCCC GGGAGCACGAUGCGGUCCUGUCAUCUGUUCGUCCCCACACGACUCGCCCGA 0762. „H32 0190. ,H0
CATTG G AG GTGTAG GGACCG GGGAGGACGAUGCGGCAUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 0763. .H32
G G CTTTCGTTCTG CCCTCCC GGGAGGACGAUGCGGGGCUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0764. _H32 1462. .H04
TTTGAAGTGGGATGGGTCCG GGGAGGACGAUGCGG UUUGAAGUGGGAUGGGUCCGCAGACGACUCGCCXGA 0765. .H32 2144. .H04
TTTG AGGGTG ATG G G ACTG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGACUGCAGACGACUCGCCCGA 0766. .H32
TTTGAAGGGGGATGGGACTG GGGAGGACGAUGCGGU UUGAAGGGGGAUGGGACUGCAGACGACUCGCCCGA 0767_ _H32 5261. _H04
G G C AG TCTTCTCG ATG TGTG GGGAGGACGAUGCGGGGCAGUGUUCUCGAUGUGUGCAGACGACUGGCCCGA 0768. _H32
TGCGTTTCGTTT'GCCCTGCC GGGAGGACGAUGCGGUGCGUU UCGUUUGCCCUGCCCAGACGACUCGCCCGA 0769. H32
ATTGGTGAAGGGAGGTACCC GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACCCCAGACGACUCGCCCGA 0770. ,H32
G ATCCG TG TCCTCTGGTGTG: GGGAGGACGAUGCGGGAUCCGUGUCCUCUGGUGUGCAGACGACUCGCCCGA 0771. _H32
G CCTTTCGTTCTGCCCTTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCU UCCCCAGACGACUCGCCCGA 0772. .H32 1302. _HQ4
AACTATCATACATATGGCTG GGGAGGACGAUGCGGAACUAUCAUACAUAUGGCUGCAGACGACUCGCCCGA 0773_ _H32
CG TTTCTTCTG G TTCGTCCC GGGAGGACGAUGCGGCGUUUCUUCUGGUUCG UCCCCAGACGACUCGCCCGA 0774. _H32 2932. .H04
TTTGAAGGGTGATGGGGGTG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGGGUGCAGACGACUCGCCCGA 0775. .H32
TTG GGCGTAG GGGG GTACCG GGGAGGACGAUGCGGU UGGGCGUAGGGGGG UACCGCAGACGACUCGCCCGA 0776. .H32
CTTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGCUUGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0777. .H32 1894. „HQ4
CAGAGGAAAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAAAAGGGAGGUUGCCAGACGACUCGCCCGA 0778. .H32
GCCTTCTGG CTTATCGTCCC GGGAGGACGAUGCGGGCCUUCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 0779. .H32 3052. _H04
TGGGCCTTCGTCTTGCTGTG GGGAGGACGAUGCGGUGGGCCUUCGUCUUGCUGUGCAGAC6ACUCGCCCGA 0780. _H32
GCCTTTCGTT6TGACCTCCC GGGAGGACGAIJGCGGGCCUUUCGUUGUGACCUCCCCAGACGACUCGCCCGA 0781. _H32
TGAGTCAGTCTTTACCGCCC GGGAGGACGAUGCGGUGAGUCAGUCUUUACCGCCCCAGACGACUC6CCCGA 0782. _H32
CGTTTCCTCTGGTTGTCCC GGGAGGACGAUGCGGCGUUUCCUCUGGUUGUCCCCAGACGACUCGCCCGA 0783 H32
# in H
HTS read variable region RNA sequences 8 in H3 selection selection Selected sequenc
ATTG GTG A AGG G AG CTACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGCUACCGCAGACGACUCGCCCGA Q784_H32
TTGATTCGTCTGTGCTCCCC GGGAGGACGAUGCGGU UGAUUCGUCUGUGCUCCf.CCAGACGACUCGCCCGA 0785_H32
TGTAGGAGGAGGAGCTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGCUACCGCAGACGACUCGCCCGA 0786_H32
CAGAG AGCAGG G AG GTTGC GGGAGGACGAU6CGGCAGAGGAGCAGGGAGGUUGCCAGACGACUCGCCCGA 07S7_H32
ATTGGTGAAGGGAGATACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGAUACCGCAGACGACUCGCCCGA G788_H32
GCCTTTCGTGCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUGCUGCCCUCCCCAGACGACUCGCCCGA 0789_H32 I849_ H04
TTTGAAGGGTGATGGGAG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGAGCAGACGACUCGCCCGA 0790_H32
TTG GG AGTAGG GGGGTACCG GGGAGGACGAUGCGGUUGGGAGUAGGGGGGUACCGCAGACGACUCGCCCGA 0791„H32 4479. .H04
TTTGGA6GGAGATG6GTCCG GGGAGGACGAUGCGGUUUGGAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 0792_H32 2470. .H04
TTTGAGGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAGGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0793_H32 2601. .H04
TTAAGGAGGAGATGGAGGTG GGGAGGACGAUGCGGUUAAGGAGGAGAUGGAGGUGCAGACGACUCGCCCGA 0794_H32
CAGAGGAGAAAGGAGGUGC GGGAGGACGAUGCGGCAGAGGAGAAAGGAGG U JGCCAGACGACUCGCCCGA 0795_H32
TCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0796_H32 0561. H04
AATTTTGGTGGAGGTGGATG GGGAGGACGAUGCGGAAUU UU6GUGGAGGUGGAUGCAGACGACUCGCCCGA 0797„Η32
ATTG GTTA AG GG AG GTACCG GGGAGGACGAUGCGGAUUGGUUAAGGGAGGUACCGCAGACGACUCGCCCGA 0798_H32
GCCTITCGTTCTGCCCTCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCUCCCCCAGACGACUCGCCCGA 0799__H32 _H04
TCCTTGTCGTCTT6CGTCC GGGAGGACGAUGCGGUCCUUGUCGUCUUGCGUCCCAGACGACUCGCCCGA 0800„H32 1813, H04
TCTGTACGGTGGTGAGG6GG G6GAGGACGAUGCGGUCUGUAC6G UGGUGAGGGGGCAGACGACUCGCCCGA 0801_H32
TGTAGGGAGGTAGTCATCGT GGGAGGACGAUGCGGUGUAGGGAGGUAG UCAUCGUCAGACGACUCGCCCGA 0802_H32
TAGGAGGAGGAGGTGTTGTG GGGAGGACGAUGCGGUAGGAGGAGGAGGUGUUGUGCAGACGACUCGCCCGA 0803_H32
TTTG AT6GG CGATGG GTCCG GGGAGGACGAUGCGGUUUGAUGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0804„H32 2147. .H04
AGGCGTCTCGTCTTATACTG GGGAGGACGAUGCGGAGGCGUCUCGUCUUAUACUGCAGACGACUCGCCCGA 0805„H32
CAGAGGCGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGCGAAGGGAGGUUGCCAGACGACUCGCCCGA 0806_H32
TTTGTCGTGTGTTCGTCCC GGGAGGACGAUGCGGU UUGUCGUCUUGUUCGUCCCCAGACGACUCGCCCGA 08Q7_H32
TTGGACTTGGAAACCGTGGC GGGAG6ACGAUGCGGU UGGACUUGGAAACCGUGGCCAGACGACUCGCCCGA 080a_H32
GGTGGTGAAGGGTTGGTCCG GGGAGGACGAUGC6GG6UG6UGAAGG6UUGGUCCGCAGACGACUCGCCCGA 0809_H32
T ATA ATT AG 6 AG G AG G TG G G GGGAGGACGAUGCGGUAUAAUUAGGAGGAGGUGGGCAGACGACUC GCCCGA 0810_H32
AGGGAGCAGGGAGTTTACCG GGGAGGACGAUGCGGAGGGAGCAGGGAGUUUACCGCAGACGACUCGCCCGA 0811 H32
# in m
NTS read variable region NA sequences it in HS selection selection Selected sequenc
TCGGGTCCTCGCATTGTGT6 GGGAGGACGAUGCGGUCGGGUCCUCGCAU UGUGUGCAGACGACUCGCCC6A 0812. _H32
GCCTTTCGTTCTGACCGCCC GGGAGGACGAUGCGGGCCU UUCGUUCUGACCGCCCCAGACGACUCGCCCGA 0813. _H32
ATTGGTGAAGGTAGGTACCG GGGAGGACGAUGCGGAUUGGUGAAGGUAGGUACCGCAGACGACUCGCCCeA 0814, .H32
TTGGGTGTACGGTGGTACCG GGGAGGACGAUGCGGUUGGG UGUACGGUGGUACCGCAGACGACUCGCCCGA 0815. .H32 3388. .H04
TTTGAAGGGAGATGGGTCCC GGGAGGACGAUGCGGU UUGAAGGGAGAUGGGUCCCCAGACGACUCGCCCGA 0816. _H32 1448. H04
T6TAGGAGGCGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAGGCGGAGGUACCGCAGACGACUCGCCCGA 0817. _H32
TTTAGGAGGAGGA6GTACCG GGGAGGACGAUGCGGUUUAGGAGCACGACGUACCGCAGACGACUCGCCCGA 0818. „H32
TTTGAAGGGCGTTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGCGUUGGGUCCGCAGACGACUCGCCCGA 0819. _H32 1620. .H04
7TCGT7TCCTTCGTCGTGTG GGGAGGACGAUGCGGUUCGUUUCCUUCG UCGUGUGCAGACGACUCGCCCGA 0820. _H32
CCAAGTCGTTCCATCGTGTG GGGAGGACGAUGCGGCCAAG UCGUUCCAUCGUGUGCAGACGACUCGCCCGA 0821. _H32
T6CCTCTGTCGTTGCTTGTG GGGAGGACGAUGCGGUGCCUCUGUCGUiJGCUUG UGCAGACGACUCGCCCGA 0822_ _H32
TGTAGGAAGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAAGAGGAGGUACCGCAGACGACUCGCCCGA 0823. .H32
TCTGGTGATGTTAGCCG GTG GGGAGGACGAUGCGGUCUGGUGAUGUUAGCCGG UGCAGACGACUCGCCCGA 0824. H32
GATGACGGCGACTCGCACTA GGGAGGACGAUGCGGGAUGACGGCGACUCGCACUACAGACGACUCGCCCGA 0825. .H32
CGACTTTTTGCCTCTTGGCC GGGAGGACGAUGCGGCGACUUUUUGCCUCUUGGCCCAGACGACUCGCCCGA 0826. _H32
CAGAGGAGAAGGGAGGTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUGCCAGACGACUCGCCCGA 0827. .H32
CAGAGGAGAAGGGAGGTTGG GGGAGGACGAUGCGGCAGAGGAG AG6GAGGUUGGCAGACGACUCGCCCGA 0828. .H32
CTTGGTGAAGGGAGGTACCG GGGAGGACGAUGCGGCUUGGUGAAGGGAGGUACCGCAGACGACUCGCCCGA 0829. _H32
GTTGCTGTAGTGCGGTGCGC GGGAGGACGAUGCGGG UUGCUGUAGUGCGGUGCGCCAGACGACUCGCCCGA 0830. _H32
TATTGGAGGTGTAGTGACCG GGGAGGACGAUGCGGUAU UGGAGGUGUAG UGACCGCAGACGACUCGCCCGA 0831. H32
TTTGAAGGGCGATGGGTCCC GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGUCCCCAGACGACUCGCCCGA 0832. .H32 0921. .H04
TTTGAAGGGG ί GGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGUGGGUCCGCAGACGAC UCGCCCGA 0833. _H32 1751. .H04
TGTAGGCGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGCGGAGGAGGUACCGCAGACGACUCGCCCGA 0834. .H32
TTTGAAGAGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAA6AGGGAUGG6UCCGCAGACGACUCGCCCGA 0835. .H32 3444. .H04
TTTGTTGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGUUGGGUGAUGGG UCCGCAGACGACUCGCCCGA 0836. .H32 2623, .H04
TT6G GTG TAG G GTG GTAGCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUAGCGCAGACGACUCGCCCGA 0837, .H32 4124. .H04
AATCG G G CCTTTTG TCACTG GGGAGGACGAUGCGGAAUCGGGCCU U UUGUCACUGCAGACGACUCG CCGA 0838. _H32
GCCTTTCGTCTGTTCGTCCC GGGAGGACGAUGCGGGCCU UUCGUCUGU UCGiiCCCCAGACGACUCGCCCGA 0839 H32 0715 H04
# in H4
NTS read variable region NA sequences # in H3 selection selection Selected sequenc
TGA6TAAGTCTGTTTCCCCC GGGAGGACGAUGCGGUGAGUAAGUCUGUUUCCCCCCAGACGACUCGCCCGA 0840_ H32
TGTGGCAAAATTACGCTGTG GGGAGGACGAUGCGGUGUGGCAAAAUUACGCUGUGCAGACGACUCGCCCGA 0841_ H32
TGACAGTTGTCTGCGCTCCA GGGAGGACGAUGCGGUGACAGUUGUCUGCGCUCCACAGACGACUCGCCCGA 0842_ H32
CAGAGGAGAAGGGCGGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGCGG UUGCCAGACGACUCGCCCGA 0843_ H32
TCCTGTCGTCTATTCGTCCC GGGAGGACGAUGC6GUCGU6UCGUCUAUUCGUCCCCAGACGACUCGCCCGA. 0844„ H32 0109 H04
CCAACAAATCATGTGTGCCC GGGAGGACGAUGCGGCCAACAAAUCAUGUG UGCCCCAGACGACUCGCCCGA 0845_ H32
AATTTGTCCTCGGTCGTGTG GGGAGGACGAUGCGGAAUUUG UCCUCGGUCG UGUGCAGACGACUCGCCCGA 0846_ H32
ATGTGTCCTTACCAGTGCCC GGGAGGACGAUGCGGAUGUGUCCU UACCAGUGCCCCAGACGACUCGCCCGA 0847_ H32
CC ATTG A ACCTCCCGTTG G C GGGAGGACGAUGCGGCCAUUGAAeCUCCCGUUGGCCAGACGACUCGCCCGA 0848_ H32
TCGATCGTTCTTGCCTACGC GGGAGGACGAUGCGGUCGAUCG UUCUUGCCUACGCCAGACGACUCGCCCGA 0849_ H32
ACCTCCAGTTGTCTGTACGC GGGAGGACGAUGCGGACCUCGAG UUGUCUGUACGCCAGACGACUCGCCCGA 0850_ H32
TTG GGTG TAGG GTG TTACTG GGGAGGACGAUGCGGU UGGGUG UAGGGUGUUACUGCAGACGACUCGCCCGA 0851_ H32
TTGGGTGTAGGGGGGTACTG GGGAGGACGAUGCGGU UGGGUGUAGGGGGGUACUGCAGACGACUCGCCCGA 0852^ H32
0
AACG TG TC G CTTTG CTG CCC GGGAGGACGAUGCGGAACGUGUCGCUUUGCUGCCCCAGACGAGUCGCCCGA 0853_ H32
TGTGGCGTGTCATCTCC6TG GGGAGGACGAUGCGGUGUGGCGUG UCAUCUCCGUGCAGACGACUCGCCCGA 085 _ H32
TTG GG AGTAG G GTGTTACCG GGGAGGACGAUGCGGU UGGGAGUAGGG UGUUACCGCAGACGACUCGCCCGA 0855_ H32
TGTAGGAGAAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGAGAAGGAGGUACCGCAGACGACUCGCCCGA 0856_ H32
TTGGGTGTAGGGAGTTACCG GGGAGGACGAUGCGGU UGGGUG UAGGGAGUUACCGCAGACGACUCGCCCGA 0857_ H32
TGTAGGAGGAGGAGGTACGG GGGAGGACGAU6CGGUGUAGGAGGAGGAGGUACGGCAGACGACUCGCCCGA 0858_ H32-
CTTGTCGTCTTGTTGTCCC GGGAGGACGAUGCGGCUUGUCGUCUU6UUG UCCCCAGACGACUCGCCCGA Q859_ H32 4515 H04
TGTAGGTGGAGGAGGTACCG GGGAGGACGAUGCGGU6UAGGUGGAGGAGGUACC6CAGACGACUCGCCCGA 0860_ H32
TTTG G AGG AG G ATG G GTCCG GGGAGGACGAUGCGGU UUGGAGGAGGAUGGGUCCGCAGACGACUCGCCCGA 0S6l_ H32
TGTAGTAGGAG -GAGGTACCG GGGAGGACGAUGCGGUGUAGUAGGAGGAGGUACCGCAGA.CGACUCGCCCGA 0862_ H32
GGATAGTCTGGTTTGCCCGC GGGAGGACGAUGCGGGGAUAGUCUGGUUUGCCCGCCAGACGACUCGCCCGA 0863_ H32
CAGAGGAGAAGGTAGGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGUAGGULGCCAGACGACUCGCCCGA 0864_ H32
CG TCCCTTCGTCTTGTG CGC GGGAGGACGAUGCGGCGUCCCUUCGUCUUGUGCaCCAGACGACUCGCCCGA 0865_ H32
TTGG6TGTAGGGTGGTGCTG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGUGCUGCAGACGACUCGCCCGA 0866„ H32
GCAATTTCGTCTAGTGTCCC GGGAGGACGAUGCGGGCAAUU UCGUCUAGUGUCCCCAGACGACUeGCCCGA 0867__ H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATGTCAACTACCC6TGACCG GGGAGGACGAUGCGGAUGUCAACUACCCGUGACCGCAGACGACUCGCCCGA 0S68_H32
GCCTTTCGTTCTGCCCCCTC GGGAGGACGAUGCGGGCCU UUCGUUCUGCCCCCUCCAGACGACUCGCCCGA 0869_H32 4240. H04
CCGTACGTTGTCTCCTGTG GGGAGGACGAUGCGGCCGUACG UUG UCUCCUGUGCAGACGACUCGCCCGA 0870_H32
TCCATCACACTAAGCCGCAC GGGAGGACGAUGCGGUCCAUCACACUAAGCCGCACCAGACGACUCGCCCGA 0871..Ή32
TGAGTCAGAATCTGCTGCCC GGGAGGACGAUGCGGUGAGUCAGAAUCUGCUGCCCCAGACGACUCGCCCGA 0872.. H32
TTTTTGGTTTTTTTTTTGCCCG GGGAGGACGAUGCGGUUUUUGGUUUUUUUUUUGCCCGCAGACGACUCGCCCGA 08?3_H32
TGACTCGTCTGTTTTGTCCC GGGAGGACGAUGCGGUGACUCG UCUGUUUUGUCCCCAGACGACUCGCCCGA 0874_H32
GCCTTTCGTTCGGACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCGGACCiJCCCCAGACGACUCGCCCGA 087S_H32
GGTAGGAGGAGGAGGTACC6 GGGAGGACGAUGCGGGGUAGGAGGAGGAGG UACCGCAGACGACUCGCCCGA 0876._H32
TATAG6A6GAGGAGGTACCG 6GGAGGACGAUGCGGUAUAGGAGGA6GAGGUACCGCAGACGACUCGCCCGA 0877_H32
TATCGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUAUCGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 0878_H32
ATTGAAGGGGGATGGGTCCG GGGAGGACGAUGCGGAUUGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 0879„H32 4338, „H04
oe TTTGTAGGGAGATGGGTCCG GGGAGGACGAUGCGGUUUGUAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 08S0_H32 2414,. .H04
o
TTTGAGGGGCGATGGGTCCG GGGAGGACGAUGCGGUUUGAGGGGCGAUGGGUCCGCAGACGACUCGCCCGA 0881_H32 3239. _H04
ATTG6TGAAGGGAGGTACT GGGAGGACGAUGCGGAUUGGUGAAGGGAGG UACUCAGACGACUCGCCCGA G882_H32
7TGGGTGTAGGGTGGTATTG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUAUUGCAGACGACUCGCCCGA 0883_H32
TTGGGTGTAGGGCGGAACCG GGGAGGACGAUGCGGUUGGGUGUAGGGCGGAACCGCAGACGACUCGCCCGA 0884 . H32 4935. .H04
TTTGAAGGGTGGTAGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGGUAGGUCCGCAGACGACUCGCCCGA 0885_H32 2841. .H0
CTTGAAGGGTGATGGGGCCG GGGAGGACGAUGCGGCUUGAAGGG UGAUGGGGCCGCAGACGACUCGCCCGA 0886_B32 2676. .H04
ATTGGTGTAGGGTGTTACCG GGGAGGACGAUGCGGAUUGGUGUAGGGUG UUACCGCAGACGACUCGCCCGA 0887_H32
CAGAGGAGAAGGGATGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAUGUUGCCAGACGACUCGCCCGA 0888„H32
TCCGCTCGCACATTCGTCTG GGGAGGACGAUGCGGUCCGCUCGCACAUUCGUCUGCAGACGACUCGCCCGA 0889„H32
TTG GG CGTAGG GTG TTACCG GGGAGGACGAUGCGGUUGGGCGUAGGGUGUUACCGCAGACGACUCGCCCGA 0890_H32
CTG G G TGT AG GGTGTT ACCG GGGAGGACGAUGCGGCUGGGUGUAGGGUGUUACCGCAGACGACUCGCCCGA 0891_H32
TTTGGGGTTCTCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGU UCUCCCGUGCCCCAGACGACUCGCCCGA 0892_H32 1259. _H04
TGTAGGAGGAAGAGGTACCG GGGAGGACGAUGC6GUGUAGGAGGAAGAGGUACCGCAGACGACUCGCCCGA 0893_H32
GTCGTCAGTTTTCCG CTCCC GGGAGGACGAUGCGGGUCG UCAGUUUUCCGCUCCCCAGACGACUCGCCCGA 0894_H32
AGTGCGATGGTCT6TGTGTG GGGAGGACGAUGCGGAGUGCGAUGGUCUGUGUGUGCAGACGACUCGCCCGA 0895 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATTGGGTGTAGGOTGGTACCG GGGAGGACGAUGCGGAUUGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0396. .H32
T6TAGGAGGAGGAGATACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGAUACCGCAGACGACUCGCCCGA 0897. .H32
ATCGTCCGTTCGTCTGCCC GGGAGGACGAUGCGGAUCGUCCGUUCGUCUGCCCCAGACGACUCGCCCGA 0898. _H32
TG ACTCGTCTGTTTC GTCC GGGAGGACGAUGCGGUGACUCG UCUGUU UCGUCCCAGACGACUCGCCCGA 0899. _H32
ATTTG TG AA G G AG GTACCG GGGAGGACGAUGCGGAUUUGUGAAGGGAGG UACCGCAGACGACUCGCCCGA 09Q0. .H32
CCTGTCGTCTTGTTCG TCCC GGGAGGACGAUGCGGCCUGUCG UCUUGUUCGUCCCCAGACGACUCGCC GA 0901. _H32
TCCTGTCGTCTGATCGTCCC GGGAGGACGAUGCGGUCCUG'JCGUCUGAUCGUCCCCAGACGACUCGCCCGA 0902. .H32 0203. _H04
TTTTGAAGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUUGAAGGG UGGUGGG UCCGCAGACGACUCGCCCGA 0903. _H32 3288. .H04
TTTGGAGGGTGGTGGGACCG GGGAGGACGAUGCGGUUUGGAGGGUGGUGGGACCGCAGACGACUCGCCCGA 0904. .H32
TTGGGAGTAGGATGGGACCG GGGAGGACGA'JGCGGUUGGGAGUAGGAUGGGACCGCAGACGACUCGCCCGA 0905. H32 4673. _R04
TTGGGTGTAGGGAGGAACCG GGGAGGACGAUGCGGUUGGGUGUAGGGAGGAACCGCAGACGACUCGCCCGA 0906. H32
TATTGGAGGTGAAGGGACCG G6GAGGACGAUGCG6UAUUGGAGGUGAAGGGACCGCAGACGACUCGCCCGA 0907. H32
GGGTGTAGGGTGGTA.CCG G6GAGGACGAUGCGGGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0908. _H32 3972. H04
C i A l l G GTGAAGGG AGGTACTG GGGAGGACGAUGCGGCUAUUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 0909. H32
GTTGGTCCGTCCTGCGTGTG GGGAGGACGAUGCGGG UUGGUCCGUCCUGCGUGUGCAGACGACUCGCCCGA 0910. .H32
AGTCCTGTCGTTTGTGGCGC GGGAGGACGAUGCGGAGUCCUGUCGUUUGUGGCGCCA6ACGACUCGCCCGA 0911. .H32
CAG AG G AG AAGG GAG GTCGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUCGCCAGACGACUCGCCCGA 0912. H32
GTCCTGTCGTCTGTTGTCCC GGGAGGACGAUGCGGGUCCUGUCGUCUGUUGUCCCCAGACGACUCGCCCGA 0913. H32 1598. _H04
GTTGAGCCTTCTTC6TTGTG GGGAGGACGAUGCGGGUUGAGCCUUCUUCGUUGUGCAGACGACUC6CCCGA 0914. .H32
GCCTTTCGTTCTGCCCTACCC GGGAGGACGAUGCGG6CCUUUCGUUCUGCCCUACCCCAGACGACUCGCCC6A 0915. H32 2174. .H04
TACCGCGTCGTTTGCTGCGG GGGAGGACGAUGCGGUACCGCGUCGUUUGCUGCGGCAGACGACUCGCCCGA 0916. .H32
TCCTGTCGTCTG CTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGCUGUCCCCAGACGACUCGCCCGA 0917. _H32 0181. H04
GCCTTTCGTTCGACCTCCC GGGAGGACGAUGCGGGCCUUUCG UUCGACCUCCCCAGACGACUCGCCCGA 0918. „H32
GCTCTTCGTTCTG ACCTCCC GGGAGGACGAUGCGGGCUCUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 0919. .H32
CAGAGGAGAAGGGAGGTTGA GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUUGACAGACGACUCGCCCGA 0920. H32
TGTAGGAGGAGGCGGTACCG GGGAGGACGAUGCG6UGUAGGAGGAGGCGGUACCGCAGACGACUCGCCCGA 0921. _H32
TG AGTCG ATGTTG CCG TGTC GGGAGGACGAUGCGGUGAGUCGAUGUUGCCGUGUCCAGACGACUC6CCCGA 0922. _H32
TAACCTATGTGTG GTTG CGC GGGAGGACGAUGCGGUAACCUAUGUGUGGUUGCGCCAGACGACUCGCCCGA 0923 H32
# in H4
HTS read variable region NA sequences # In H3 selection selection Selected sequenc
TTTGAAGGGTTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUUGGGUCCGCAGACGACUCGCCCGA 0924. H32 1474. H04
TTTGAATGGAGATG G GTCCG GGGAGGACGAUGCGGUU UGAAUGGAGAUGGGUCCGCAGACGACUCGCCCGA 0925. „H32 3941. .HQ4
GTCCTG TC G T T G TTCG TCCG GGGAGGACGAUGCGGGUCCUGUCG UCUGUUCGUCCGCAGACGACUCGCCCGA 0926. „H32 3512, „H04
CCCTATGTTCTCCCGTGCCC GGGAGGACGAUGCGGCCCUAUGUUCUCCCGUGCCCCAGACGACUCGCCCGA 0927. _H32
TTTGGAGGATGGTGGGACCG GGGAGGACGAUGCGGUU UGGAGGAUGGUGGGACCGCAGACGACUCGCCCGA 0928. .H32
ATTGG AG AAG GGTGG G ACTG GGGAGGACGAUGCGGAUUGGAGAAGGG UGGGACUGCAGACGACUCGCCCGA 0929, .H32
ATTGGTGAGGGGAGGQACTG GGGAGGACGAUGCGGAUUGGUGAGGGGAGGGACUGCAGACGACUCGCCCGA 0930. JH32
TTGGGTGTAGGGTGGTACC GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUACCCAGACGACUCGCCCGA 0931. _H32
TTGGGAGTGGGGTGGGACCG GG6A6GACGAUGCGG UUGGGAGUGGGGUGGGACCGCAGACGACUCGCCCGA 0932, _H32
ATTGG AGAAG GGAGG GACCG GGGAGGACGAUGCGGAUUGGAGAAG6GA6GGACCGCAGACGACUCGCCCGA 0933, _H32
TGTAGGATGAGGAGGTACCG GGGAGGACGAUGCGG UGUAGGAUGAGGAGG UACCGCAGACGACUCGCCCGA 0934. .H32
TTTGAAGGGTGGTGGGCCCG GGGAGGACGAUGCGG UUUGAAGGGUGGUGGGCCCGCAGACGACUCGCCCGA 0935. .H32 3287. .H04
GTCTTCTGG CTATCGTCCC GGGAGGACGAUGCGGGUCUUCUGGCUAUCGUCCCCAGACGACUCGCCCGA 0936. _H32 5181. .H04
GTGATTGCTGCC6TCCTGTG GGGAGGACGAUGCGGGUGAUUGCUGCCGUCCUGUGCAGACGACUCGCCCGA 0937. _H32
GTT6TCATATACTCCCCCCC GGGAGGACGAUGCGGGUUGUCAUAUACUCCCCCCCCAGACGACUCGCCCGA 0938, _H32
TTGGGAGTAGGGTGGTACTG GGGAGGACGAUGCGG UUGGGAGUAGGGUGGUACUGCAGACGACUCGCCCGA 0939. .H32
GCCTTTCGTTCTACCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUACCUCCCCAGACGACUCGCCCGA 0940. .H32 2175. .H04
TCCTTGTCGTCTTGTCCC GGGAGGACGAUGCGGUCCUUG UCGUCUUGUCCCCAGACGACUCGCCCGA 0941. _H32 1194. .H04
CATAGTCCTGTCGTGTTGCA GGGAGGACGAUGCGGCAUAGUCCUGUCGUGUUGCACAGACGACUCGCCCGA 0942. _H32
TATCGTTACCCGTTTGCCC GGGAGGACGAUGCGGUAUCGUUACCCGUUUGCCCCAGACGACUCGCCCQA 0943. _H32
CGATTTGTCTTCCACGTGTG GGGAGGACGAUGCGGCGAUUUGUCUUCGACGUGUGCAGACGACUCGCCCGA 0944. .H32
TTTGAAGGGTGGTGGGTCTG GGGAGGACGAUGCGGUUUGAAGGGUGGUGG6UCUGCAGACGACUCGCCC A 0945. .H32 3814. H04
TTTGAAGGGAGATGGTTCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGG UUCCGCAGACGACUCGCCCGA 0946. .H32 3179. ..H04
TATTGGAGGT6TTGGGACCG GGGAGGACGAUGCGGUAU UGGAGGUGUUGGGACCGCAGACGACUCGCCCGA 0947. .H32
TTG G GTGTAGG GAG GCACCG GGGAGGACGAUGCGGUUGGG UGUAGGGAGGCACCGCAGACGACUCGCCCGA 0948. _H32
TGTAGGAGGAGGAGGTATCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGGUAUCGCAGACGACUCGCCCGA 0949. _H32
TTGGGAGTGGGGTGGTACCG GGGAGGACGAUGCGGUUGGGAGUGGGGUGGUACCGCAGACGACUCGCCCGA 0950. _H32 4940. _H04
TATTGGAGGTAGGGACCG GGGAGGACGAUGCGGUAU UGGAGGUAGGGACCGCAGACGACUCGCCC A 0951 H32
# in H4
HTS read variable region R A sequences » in H3 selection selection Selected sequenc
CAGAAGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAAGAGAAGGGAGGUUGCCAGACGACUCGCCCGA 0952_ .H32
TTCTCAGTAT7GGTCGTGGC GGGAGGACGAUGCGGU UCUCAGUAUUGG UCGUGGCCAGACGACUCGCCCGA 0953. _H32
TGGCCTGTCGTCTACGTGTG GGGAGGACGAUGCGGUGGCCUGUCGUCUACGUGUGCAGACGACUCGCCCGA 0954_ _H32
ACGTGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGACGUGGUG UAGGGUGGUACCGCAGACGACUCGCCCGA 0955. H32
GTCGATTCGTTTGTCCTGCC GGGAGGACGAUGCGGGUCGAU UCGUU UGUCCUGCCCAGACGACUCGCCCGA 0956. H32
TCCTGTGTTGTCTTACACTG GGGAGGACGAUGCGGUCCUGUGUU6UCU UACACUGCAGACGACUCGCCCGA 0957_ .H32
GCCTTACGTTCTGACCTCCC GGGAGGACGAUGCGGGCCUUACGUUCUGACCUCCCCAGACGACUCGCCCGA 0958_ .H32
TG CG AGTGTTTGCTGTG CCC GGGAGGACGAUGCGGUGCGAGUGUUUGCUGUGCCCCAGACGACUCGCCCGA 0959_ _H32
ATCCTCACCCTGTCGTGCTG GGGAGGACGAUGCGGAUCCUCACCCUGUCGUGCUGCAGACGACUCGCCCGA 0960_ .H32
ATGCGAGGGAGGAGGGTGTG GGGAGGACGAUGCGGAUGCGAGGGAGGAGGGUG UGCAGACGACUCGCCCGA 0961. _H3.2
ATTGGTGAAGGGGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGGGUACUGCAGACGACUCGCCCGA 0962. .H32
TCGTTCCCTCGTCGTGTG GGGAGGACGAUGCGGUCGU UCCCUCGUCGUGUGCAGACGACUCGCCCGA 0963_ _H32
CTTGTCGTCTTGCTCGTCCC GGGAGGACGAUGCGGCUUG UCG'JCUUGCUCG UCCCCAGACGACUCGCCCGA 0964_ _H32
TCCTGTCGTCTGTTCGTCAC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCACCAGACGACUCGCCCGA 0965_ _H32 0240, HG4
ATTCCTCCGCGTGTCGTTTG GGGAGGACGAUGCGGAUUCCUCC6CGU6UCGU UUGCAGACGACUCGCCCGA 0966_ _H32
ATTGGAGAAGGGTGGTACTG G6GAGGACGAUGCGGAUUGGAGAAGGGUGGUACUGCAGACGACUCGCCCGA 0967_ .H32
AATTATAGGTGG ACTFG CAT GGGAGGACGAUGCGGAAUUAUAGGUGGACUUGCAUCAGACGACUCGCCCGA 0968, JH32
ATTGGAGGAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGAGGAGGGAGGGACUGCAGACGACUCGC.CCGA 0969_ . H32
GCTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 0970_ . H32 0518_ .H04
TTTGGGGTTTTCCCGTTCCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCG UUCCCCAGACGACUCGCCCGA 0971. .H32 1008_ ,H04
ATCTCATGGGGTGATGTCCC GGGAGGACGAUGCGGAUCUCAUGGGGUGAUGUCCCCAGACGACUCGCCCGA 0972. _H32
ATTGGTGAAGGGAGGTATG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUAUGCAGACGACUCGCCCGA 0973. .H32
TTGGGTGTGGGTGGTACCG GGGAGGACGAUGCGGU UGGGUGUGGGUGGUACCGCAGACGACUCGCCC6A 0974. .H32
TCCTGTCGTCTGTTCCTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCCUCCCCAGACGACUCGCCCGA 0975. H32 0310. _H04
TTTGAAGGGGGATGGGTCGG GGGAGGACGAUGCGGU UU6AAGGGGGAUGGGUCGGCAGACGACUCGCCCGA 0976_ ,H32 2977. _H04
TTTGAAG G GCTATGG GTCCG GGGAGGACGAUGCGGU UUGAAGGGCUAUGGGUCCGCAGACGACUCGCCCGA 0977_ ,H32 1643_ H04
TTTGAAGGTAGATGGGTCCG GGGAGGACGAUGCGGU UUGAAGGUAGAUGGGUCCGCAGACGACUCGCCCGA 0978. _H32 3136, _H04
TGTAGGGAGGAAGTTATCGT GGGAGGACGAUGCGGUGUAGGGAGGAAGUUAUCGUCAGACGACUCGCCCGA 0979 H32
# tn H4
HTS read variable region RNA sequences ft in H3 selection selection Selected sequenc
TTGGGTGAAGGGCGGTACCG GGGAGGACGAUGCGGUUGGGUGAAGGGCGGUACCGCAGACGACUCGCCC6A 0980_ .H32
TGTAGG6AGGTAGTTATCGG GGGAG6ACGAUGCGG UGUAGGGAGGUAGUUAUCGGCAGACGACUCGCCCGA 0981_ JH32
CAGAGGAGAAGGGAGGTTG GGGAGGACGAUGCGGCAGAGGAGAAGGGAGG UUGCAGACGACUCGCCCGA 0982, „H32
GTTTGCGTCTGACCTGTCCC GGGAGGACGAUGCGGGUU UGCGUCUGACCUGUCCCCAGACGACUCGCCCGA 0983 .H32
CTTGTCGTCTTGTTTCTCCC GGGAGGACGAUGCGGCUUGUCGUCUUGUUUCLCCCCAGACGACUCGCCCGA 0984. ,H32
TAGCGTCGTCTGGCCATGTG GGGAGGACGAUGCGGUAGCGUCGUCUGGCCAUGUGCAGACGACUCGCCCGA G985. _H32
TCATGTCCTGCCACGTTGCC GGGAGGACGAUGCGGUCAUGUCCUGCCACGUUGCCCAGACGACUCGCCCGA 0986. _H32
TCCCAAATG CCTCCCGTGTG GGGAGGACGAUGCGGUCCCAAAUGCCUCCCGUGUGCAGACGACUCGCCCGA 0987. „H32
TCGTTTGGTCTCTGGTGCCC GGGAGGACGAUGCGGUCGUUUGG UCUCUGG UGCCCCAGACGACUCGCCCGA 0988, _H32
TGTAGGAGTAGGA6GTACCG GGGAGGACGAUGCGGUGUAGGAGUAGGAGGUACCGCAGACGACUCGCCCGA 0989. _H32
ACTG ATATC CTCCC G TTG G C GGGAGGACGAU6CGGACUGAUAUCCUCCCGUUGGCCAGACGACUCGCCCGA 0990_ .H32
AGTGGGCTGTGGTGTACGTG GGGAGGACGAUGCGGAGUGGGCUGUGGUG UACGUGCAGACGACUCGCCCGA 0991_ _H32
90 TTTGAAGGGCGATGGGTCAG GGGAGGAC6AUGCGGUUUGAAGGGCGAUGGGUCAGCAGACGACUCGCCCGA 0992. ^H32 3178. H04
4-
TGTAAGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAAGAGGAGGAGGUACCGCAGACGACUCGCCCGA 0993. _H32
GTCCTGTTGTCTGTTCGTCCC GGGAGGACGAUGCGGG UCCUGUUGUCUGUUCGUCCCCAGACGACUCGCCCGA 0994. .H32 3384_ H04
AATTCGTGCTTCGCGGTCGC GGGAGGACGAUGCGGAAUUCGUGCUUCGCGG UCGCCAGACGACUCGCCCGA 0995. .H32
TTGGGTGTAGGGTGGTACG GGGAGGACGAUGCGGU UGGG UGUAGGGUGGUACGCAGACGACUCGCCCGA 0996. _H32 4562. H04
TTGGGAGTAGGAT6GTACCG GGGAGGACGAUGCGGU UGGGAGUAGGAUGGUACCGCAGACGACUCGCCCGA 0997. _H32
TTTGAAGGGTGGTGGTTCCG GGGAGGACGAUGCGGU UUGAAGGGUGGUGGUUCCGCAGACGACUCGCCCGA 0998. .H32 3714. H04
GCGG GGTGTAGGGTG GTACCG GGGAGGACGAUGCGGGCGGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 0999. .H32 3126. H04
TATTGGAGGTGCAGGGACCG G6GAGGACGAUGCGGUAUUGGAGGUGCAGGGACCGCAGACGACUCGCCCGA 1000. _H32
TTCGAAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUCGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1001. H32 3584. H04
TGTAG GG AG G GAG TTATCGT GGGAGGACGAUGCGGUGUAGGGAGGGAGUUAUCGUCAGACGACUCGCCCGA 1002. .H32
CCGCTTCGTCACG'GCTGTG GGGAGGACGAUGCGGCCGCU UCGUCACGUGCUG JGCAGACGACUCGCCCGA 1003. _H32
CG ACTCCTCTG GTTCGTCCC GGGAGGACGAUGCGGCGACUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 1004. H32 3155_ H04
ATCCTCGAACGTTGTGTCGC GGGAGGACGAUGCGGAUCCUCGAACGUUGUGUCGCCAGACGACUCGCCCGA 1005. H32
TTAGCCTCCCGACTGCTCGC GGGAGGAC6AU6CGGUUAGCCUCCCGACUGCUCGCCA6ACGACUCGCCC6A 1006. _H32
AATCCCGTCGTCTTGCGCGC GGGAGGACGAUGCGGAAUCCCGUCGUCUUGCGCGCCAGACGACUCGCCCGA 1007 H32
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
CCGC7GTCT7CCG TAGTCTG GGGAGGACGAUGCGGCCGCUGUCUUCCGUAGUCJGCAGACGACUCGCCCGA 1008. _H32
TGTAGGAGGAGGATGTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAUGUACCGCAGACGACUCGCCCGA 1009. „H32
CCATATTGTCCCGTCTGCTG GGGAGGACGAUGCGGCCAAJAUUGUCCCGUCUGCUGCAGACGACUCGCCCG 10 0. _H32
TTAGCCGTTCTGGTGTTGGC GGGAGGACGAUGCGGU UAGCCGUUCUGGUG ULGGCCAGACGACUCGCCCGA 1011. .H32
TCCTGTCGTCCTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCCUUCGUCCCCAGACGACUCGCCCGA 1012. H32
TCCTGGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGGUCUGUUCGUCCCCAGACGACUCGCCCGA 1013.. H32 3961. .H04
TCGACATACGTCTGTGCCCC GGGAGGACGAUGCGGUCGACAUACGUCUG UGCCCCCAGACGACUCGCCCGA 1014. .H32
TCAGAGAGCCTCCTGCTCCC GGGAGGACGAUGCGGUCAGAGAGCC'UCCUGCUCCCCAGACGACUCGCCCGA 1015. .H32
ATAGTTGGACTGGTGCTGTG GGGAGGACGAUGCGGAUAGUUGGACUGGUGCUGUGCAGACGACUCGCCCGA 1016. _H32
CATTTTT6GTTGGACAGCTG GGGAGGACGAUGCGGCAUU UUUGGUUGGACAGCUGCAGACGACUCGCCCGA 1017. _H32
ATCCTCCTGGTCGTGGTGTC GGGAGGACGAUGCGGAUCCUCCUGGUCGUGGUGUCCAGACGACUCGCCCGA 1018. H32
TGTGTCGTC CAC A ATGTG TG GGGAGGACGAUGCGGUGUGUCGUCCACAAUGUGUGCAGACGACUCGCCC6A 1019. .H32
AGCGTAGTTGCATGGTGCCC GGGAGGACGAUGCGGAGCGUAGUUGCAUGCUGCCCCAGACGACUCGCCCGA 1020. _H32
CCTGTTGTCTGATGCGCGC GGGAGGACGAUGCGGCCUGUUGUCUGAUGCGCGCCAGACGACUCGCCCGA 1021. .H32
GCCTTTCGTTCTGTCTCCC GGGAGGACGAUGCGGGCCU UUCGUUCUG UCUCCCCAGACGACUCGCCCGA 1022. .H32 3S41_ .H04
TCCTGTCGTCTGTTCGACCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGACCCCAGACGACUCGCCCGA 1Q23_ H32 0289. _H04
TGCCGCGATACCATGCTCGC GGGAGGACGAUGCGGUGCCGCGAUACCAUGCUCGCCAGACGACUCGCCC6A 1024, _H32
TGACTCGTCTGTTTCGTCCG GGGAGGACGAUGCGGUGACUCGUCUGUUUCGUCCGCAGACGACUCGCCCGA 1025. H3
TCCTGTCGTCTGTTAGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGU UAGUCCCCAGACGACUCGCCCGA 1026. H32 0225. B04
TCCTTTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUUUCGUCUGU UCG UCCCCAGAeGACUCGCCCGA 1027. .H32 0230. HQ4
TTTGAAGAGAGATGGGTCCG G6GAGGACGAUGCGGUUUGAAGA6AGAUGG6UCCGCAGACGACUCGCCCGA 1028. H32 3006. H04
TTTGAAGGGTGATGGGTCCCG GGGAGGACGAUGCGGUUUGAAGGG UGAUGGGUCCCGCAGACGACUCGCCCGA 1029. H32 3154. „H04
ATTGTTGAAGGGAGGTACCG GGGAGGACGAUGCGGAUUGUUGAAGGGAGGUACCGCAGACGACUCGCCCGA I030_ .H32
CTTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGCUUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1031. _H32
TGTAGGAGGAGGAGGTACAG GGGAGGACGAUGCGGUGUAGGAGGAGGAGGUACAGCA6ACGACUCGCCCGA 1032. „H32
TACTGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUACUG6AGGUGUAGGGACCGCAGACGACUCGCCCGA 1033. ,H32 4773. HQH
TTTGGTGTAGG6AGGTACCG G6GAGGACGAUGCGGUUUGGUGUAGGGAGGUACCGCAGACGACUCGCCCGA 1034_ JH32
TTTGAAGGGTGATGGTGCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGUGCCGCAGACGACUCGCCCGA 1035 H32 2513 H04
# in H4
HTS read variable region R A sequences # in H3 selection selection Selected sequenc
7TG6GGGGAGGGTGGTACCG GGGAGGACGAUGCGGU UGGGGGGAGGGUGGUACCGCAGACGACUCGCCCGA 1036_H32
TATTGGAGGTGTAGGGACGG GGGAGGAC.GAUGC6GUAUUGGAGGUGUAGGGACGGCAGACGACUCGCCCGA 1037_ H32
AGTAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGAGUAGGAGGAGGAGG UACCGCAGACGACUCGCCCGA 1Q38_H32
TTGGGTGTAGGGGGGTTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGGUUACCGCAGACGACUCGCCCGA 1039„H32
ATAG 6G AG AAGG TG AACTG GGGAGGACGAUGCGGAUAGGGAGAAGGGUGAACUGCAGACGACUCGCCCGA 1G40_H32
TTGGGTGTAGGGGGGAACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGGAACCGCAGACGACUCGCCCGA I0 1_H32 4144 H04
TAAGATGGTGAGCACGTGTC GGGAGGACGAUGCGGUAAGAUGGUGAGCACGUGUCCAGACGACUCGCCCGA 1042„H32
TTCGTTTTGCTCCCCGTGTG GGGAGGACGAUGCGGUUCG UUUUGCUCCCCGUGUGCAGACGACUCGCCCGA 1Q43„H32
GAGGTGTCTGGATGTCTGTG GGGAGGACGAUGCGGGAGGUGUCUGGAUGUCUGUGCAGACGACUCGCCCGA 1044_H32
CATTGTCCCGGTCGGTACTG G6GAGGACGAUGCGGCAUUGUCCCGGUCGGUACUGC.AGACGACUCGCCCGA 1Q45„H32
TACCGCTCA CTTGTCG TGTG GGGAGGACGAUGCGGUACCGCUCACUUGUCGUGUGCAGACGACUCGCCCGA 1046_H32
ATGGGTCGTCTTTACCTCCC GGGAGGACGAUGCGGAUGGGUCGUCUUUACCUCCCCAGACGACUCGCCCGA 1047_H32
TG GTCTCTG G TTCTGTGG G C GGGAGGACGAUGCGGUGGUCUCUGG'JUCUG UGGGCCAGACGACUCGCCCGA 1048_H32
ATGATGTTGTCCCATGTGTG GGGAGGACGAUGCGGAUGAUG UUG UCCCAUGUGUGCAGACGACUCGCCCGA 1049_H32
TGTCGAGTTTTTTGGTGCCC GGGAGGACGAUGCGGUGUCGAGUUUUU UGG UGCCCCAGACGACUCGCCCGA 1050_H32
TCCTGTAGTCTGTTCGTCCC G G G AGG ACG A U 6CG G U CQJ G U AG U CU G U U CG U CCCCAG ACGACU CG C CCG A 1051_H32 0291 H04
ATGTGTGAGATGCCCTGCGC GGGAGGACGAU6CGGAUGUGUGAGAUGCCCUGCGCCAGACGACUCGCCCGA 1052_H32
TCCTGTCTTGTCGTTGTCCC GGGAGGACGAUGCGGUCCUGUCUUGUCGUUG UCCCCAGACGACUCGCCCGA 1053_H32
ACGTTGTCCCACTGT6TCGC GGGAGGACGAUGCGGACGU UGUCCCACUGUGUCGCCAGACGACUCGCCCGA 1054_H32
GCCTTTGGTTCTGACCTCCC GGGAGGACGAUGC6GGCCUUUGGUUCUGACCUCCCCAGACGACUCGCCCGA 1055.. H32
TTTGATGGTGATGGGTCCG GGGAGGACGAUGC6GUUUGAU6GUGAUGGGUCCGCAGACGACUCGCCCGA 1056_ H32 3850_H04
TTTGGGGTTTTCCGTGCCC GGGAGGACGAUGCGGUUUGGGGU UUUCCGUGCCCCAGACGACUCGCCCGA 1057_H32 2646 H04
ATTGGTGAAGGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGGAGGUACUGCAGACGACUCGCCCGA 1058_H32
TGAGTGGTTATGGACCGGTG GGGAGGACGAUGCGGUGAG UGGUUAUGGACCGGUGCAGACGACUCGCCCGA 1059_H32
TCATGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCAUGUCG UCUGUUCGUCCCCAGACGACUCGCCCGA 1Q60_H32 0501_H04
TTTGAAGAGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGAGUGGUGGGUCCGCAGACGACUCGCCCGA 1061_H32 4G31_H04
TTTGGAGGGGGATGGGACTG GGGAGGACGAUGCGGUUUGGAGGGGGAUGGGACUGCAGACGACUCGCCCGA 1062_H32
TTTGAAGGGTGATGGGTGCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUGCCGCAGACGACUCGCCCGA 1063 H32 1993 H04
it in m
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATAGGGAGAAGGGAGAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGAGAACCGCAGACGACUCGCCCGA 1064, H32
TTTGGAGGGGGGTGGGACTG GGGAGGACGAUGCGGUUUGGAGGGGGGUGGGACUGCAGACGACUCGCCCGA 1065. „H32
TTTGAAGGGGGATGTGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGUGUCCGCAGACGACUCGCCCGA 1066. .H32 3351 ^H0
TTTGAAGGGGGAGGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAGGGGUCCGCAGACGACUCGCCCGA 106?_ .H32 3007. ,H04
TTGGGTGTAGGGGGGGACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGGGGACCGCAGACGACUCGCCCGA 1068_ _H32
TATTGGTGGTGTAGGGACCG GGGAGGACGAUGCGGUAUUGGUGGUGUAGGGACCGCAGACGACUCGCCCGA 1069, _H32
TATTTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUAUUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 1070. _H32
ATTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGAUUGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 1071. „H32 3256. _H04
TTTGAAGGGGGATGTGTCCG GGGAGGACGAUGCGG UUUGAAGGGCGAUGUGUCCGCAGACGACUCGCCCGA 1072. _H32 2408. _H04
TATTGGAGGTGTAGGGACCA GGGAGGACGAUGCGGUAUUGGAGGUGUAGGGACCACAGACGACUCGCCCGA 1073. _H32
TCCTGTCGTCTGTTCGTGCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUGCCCAGACGACUCGCCCGA 1074. _H32 0152. _H04
TTCGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGUUCGAAGGGCGAUGGG UCCGCAGACGACUCGCCCGA 1075_ ,H32 1576. _H04
TGATTTCCTCGTTGTCT6TG GGGAGGACGAUGCGGUGAUUUCCUCGUUGUCUG UGCAGACGACUCGCCCGA
oe 1076. .H32
T.GAGTGCCTGCGACCGTGTC GGGAGGACGAUGC6GUGAG UGCCUGCGACC6UGUCCAGACGACUCGCCCGA 1077. ,H32
CCATTAGGAGTAGGGAGGTA GGGAGGACGAUGC6GCCAL UAG6AGUAGGGAGGUACAGACGACUCGCCCGA 1078. H32
GTTGCAGTCTGTCCGTGTG GGGAGGACGAUGCGGG UUGCAGUCUGUCCGUGUGCAGACGACUCGCCCGA 1079. .H32
TCCTGTCGTCTGTTCGTCGC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUCGCCAGACGACUCGCCCGA 1080. .H32 0294. „H04
CGTCGTAATGGTTTGTGGTC GGGAGGACGAUGCGGCGUCGUAAUGGUU U6UGGUCCAGACGACUCGCCCGA 1081. _H32
AATGTCGAACCACTGCTCCC GGGAGGACGAUGCGGAAUG UCGAACCACUGCUCCCCAGACGACUCGCCCGA 1082. ,H32
ACAGTCGTGTCTGGTTTGTA GGGAGGACGAUGCGGACAG UCG UGUCUGGUUIJGUACAGACGACUCGCCCGA 1083. H32
CGTTTCCTCTGGTTCGTTCC GGGAGGACGAUGCGGCGUUUCCUCUGGUUCG UUCCCAGACGACUCGCCCGA 1084. _H32
AGAGTCGCCGTGTTCCTGTG G6GAGGACGAUGCGGAGAGUCGCCGUGUUCCUGUGCAGACGACUCGCCCGA 1085. H32
AACCGTGCGGATCTCCTGTG GGGAGGACGAUGCGGAACCG UGCGGAUCUCCUGUGCAGACGACUCGCCCGA 1086. .H32
ACATGATTAACTACTGTGCC GGGAGGACGAUGCGGACAUGAUUAACUACUGUGCCCAGACGACUCGCCCGA 1087. .H32
CAGAGGAGAAGGGAAGTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAAGU UGCCAGACGACUCGCCCGA 1088. .H32
TGGATCGCTGGCTTATGCGC GGGAGGACGAUGCGGUGGAUCGCUGGCUUAUGCGCCAGACGACUCGCCCGA 1089_ H32
GCCTTTCCTTCTGACCTCCC GGGAGGACGAUGCGGGCCL UUCCUUCUGACCUCCCCAGACGACUCGCCCGA 1090. .H32
ATCGTTACCTTCTGCCTCCC GGGAGGACGAUGCGGAUCGUUACCUUCUGCCUCCCCAGACGACUCGCCCGA 1091 H32
8 in H4
HTS read variable region R A sequences # In H3 selection selection Selected sequenc
CAAGTC6TTCGTAACCTGTG GGGAGGACGAUGCGGCAAG UCG UUCGUAACCUGUGCAGACGACUCGCCCGA 1092. H32
CAG GTTGTGTGAGTGTG CCC GGGAGGACGAUGCGGCAGG UUGUGUGAGUGUGCCCCAGACGACUCGCCCGA 1093„ ,H32
ACAGCACTTCACGCTGTTGC GGGAGGACGAUGCGGACAGCACU UCACGCUGUUGCCAGACGACUCGCCCGA 1094. H32
AACCCATTGTCCTCG CTGCC GGGAGGACGAUGCGGAACCCAUUGUCCUCGCiJGCCCAGACGACUCGCCCGA 1095. _H32
AGGA6AGGGGTTCGTCTTCC GGGAGGACGAUGCG6AGGAGAGGGGUUCGUCUUCCCAGACGACUCGCCC6A 1096. H32
TTTGGGGTTTTCCCGTGCTC GGGAGGACGAUGCGGUUUGGGGUUUUCCCG UGCUCCAGACGACUCGCCCGA 1097. .H32
TTTGGGGTTTTCCCGTGCCT GGGAGGACGAUGCGGUUUGGGGUUU UCCCG UGCCUCACACGACUCGCCCGA 1098. H32 3792. .HG4
CGTCTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGCGUCUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 1099„ H32 3805_ „HG4
CCAATCGTCTCCGTGTTGTG GGGAGGACGAUGCGGCCAAUCGUCUCCGUGUUGUGCAGACGACUCGCCCGA 1100_ H32
TTTGGAGAAG6GAGGGACTG GGGAGGACGAUGCGGUUUGGAGAAGGGAGGGACUGCAGACGACUCGCCCGA 1101_ H32
TTTGATTCGTCTCCTGTGCC GGGAGGACGAUGCGGUUUGAU UCGUCUCCUGUGCCCAGACGACUCGCCCGA 1102. H32
TGCGCTCCTCTAGTTGTCTG GGGAGGACGAUGCGGUGCGCUCCUCUAGUUGUCUGCAGACGACUCGCCCGA 1103. H32
ae TAACCATAAATGATGCGCTG GGGAGGACGAUGCGGUAACCAUAAAUGAUGCGCUGCAGACGACUCGCCCGA 1104_ H32
CTTGAAGGGTGATGGGTCCC GGGAGGACGAUGCGGCUUGAAGGG UGAUGGGUCCCCAGACGACUCGCCCGA 1105. .H32 1606. _H04
GTTGGTGAAGGGAGGTGCTG GGGAGGACGAUGCGGG UUGGUGAAGGGAGG UGCUGCAGACGACUCGCCCGA 1106. .H32
TTTG GGGTGGTGGATCCG GGGAGGACGAUGCGGU UUGAAGGGUGG UGGAUCCGCAGACGACUCGCCCGA 1107. H32 4094. _H04
ATTGTG A GGAGG TACCG GGGAGGACGAU6CGGAUUGUGAAGGGAGGUACCGCAGACGACUCGCCCGA 1108. _H32
ATTGGTGCAGGGAGGTACCG GGGAGGACGAUGCGGAUUGGUGCAGGGAGGUACCGCAGACGACUCGCCCGA 1109. _H32
ATTGGAGAAG G GAGGCACTG GGGAGGACGAUGCGGAUUGGAGAAGGGAGGCACUGCAGACGACUCGCCCGA 1110.. .H32
TTTGAAGGATGATGGGACCG GGGAGGACGAUGCGGU UUGAAGGAUGAUGGGACCGCAGACGACUCGCCCGA 1111. H32 3861. _"H04
GCCTTGCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUGCGUUCUGCCCUCCCCAGACGACUCGCCCGA 1112. H32 1886. JH04
GTTGAAGGGAGATGGGTCCG GGGAGGACGAUGCGGGUUGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1113. .H32 2390. .H04
TTTGAGGGGAGATGGGTCCG GGGAGGACGAUGCGGUUUGAGGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1114. .H32 2221. _H04
TTTGAAGGGCGATGGTTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGUUCCGCAGACGACUCGCCCGA 1115. H32 2875. _H04
TTTTG AG GGGG ATGGGTCCG GGGAGGACGAUGCGGUUUUGAAGGGGGAUGGG UCCGCAGACGACUCGCCCGA 1116. H32 3623. _H04
AGA6TTGTCTATGCTGCCCC GGGAGGACGAUGCGGAGAGUUGUCUAUGCUGCCCCCAGACGACUCGCCCGA 1U7_ H32
CAGAGGATAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAGGAUAAGGGAGG U UG CCAGACGACUCG CCCGA 1118. _H32
ATGTCATGTG AATG CCGTG C GGGAGGACGAUGCGGAUGUCAUGUGAAUGCCGUGCCAGACGACUCGCCCGA 1119 H32
if in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TCTTGTCGTCTG CTCG TCCC G6GAGGACGAUGCGGUCUUG UCGUCUGCUCGUCCCCAGAC6ACUCGCCCGA 1120_H32 4532. HG4
AGAGTCCTACGCCTGTTGTG GGGAGGACGAUGCGGAGAGUCCUACGCCUGUUGUGCAGACGACUCGCGCGA 1121_H32
TGTG CGTTCTCCGTGTTGTG GGGAGGACGAUGCGGUGUGCG UUCUCCGUGU UGUGCAGACGACUCGCCCGA 1122_ H32
GTTG AAGGGTGATGGGTCCC GGGAGGACGAUGCGGGUUGAAGGG UGAUGGGUCCCCAGACGACUCGCCGGA 1123_H32 1674. .HQ4
T CACCCTGG CTCTCGTCTG GGGAGGACGAUGCGGUACACCCUGGCUCUCGUCUGCAGACGACUCGCCCGA 1124„H32
CTACGTCAGTCTGCTGGTGC GGGAGGACGAUGCGGCUACG UCAGUCUGCUGGUGCCAGACGACUCGCCCGA 1125_H32
GCCTTTCGTGTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUG UGCCCUCGCCAGACGACUCGCCCGA 1126_H32
CAGAGTAGAAGGGAGGTTGC GGGAGGACGAUGCGGCAGAG UAGAAGGGAGGUUGCCAGACGACUCGCCCGA 1127_H32
T AAG TCCGC AGTG "7 GG C C G GGGAGGACGAUGCGGUAAGUCCGCAGUGUUGGCCGCAGACGACUCGCCCGA 1128_H32
GCCTCGTTCT6CCCTCCC GGGAGGACGAUGCGGGCCUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 1129_H32 1378. _H04
GTGGCCGTCTTCGTGTTGTG GGGAGGACGAUGCGGGUGGCCGUCUUCGUGUUGUGCAGACGACUCGCCCGA 1130_H32
TTGGGGGTAGGGTGGTACTG GGGAGGACGAUGCGGUUGGGGGUACGGUGGUACUGCAGACGACUCGCCCGA 1131_ H32
TACCTCTTCCT6TGCCTCCC GGGAGGACGAUGCGGUACGUCUUCCUGUGCCUCCCCAGACGACUCGCCCGA I132„H32
ATTTTGGAGAAGGGAGGTG GGGAGGACGAUGCGGAUUUUGGAGAAGGGAGGUGCAGACGACUCGCCCGA 1133.„H32
CTAGTTGCATGCCACCTGTG GGGAGGACGAUGCGGCUAGU UGCAUGCCACCUC UGCAGACGACUCGCCCGA 1134_H32
ATTG GTG AAGGG AG GTATCG GGGAGGACGAUGCGGAUUGGUG AAGGG AGGUAUCGCAGACGACUCGCCCGA 1135_ H32
ATGGGTGTAGGGAGGTACCG GGGAGGACGAUGCGGAUGG6UGUAGGGAGGUACCGCAGACGACUCGCCCGA 1136_ H32
TTTGAAGGGGGAAGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAAGGGUCCGCAGACGACUCGCCCGA 1137_H32 4369. J-104
ATTG GTGAAGGG GGTCCCG GGGA6GACGAUGCGGAUUGGUGAAGGGAGGUCCCGCAGACGACUCGCCCGA 1I38...H32
TTTG AAG GTCG ATG GGTCCG GGGAGGACGAUGCGGUUUGAAGGUCGAUGGG UCCGCAGACGACUCGCCCGA 1139_H3Z 2256. .H04
TCCACCTCGTATTCGTG ccc GGGAGGACGAUGCGGUCCACCUCGUAUUCGUGCCCCAGACGACUCGCCCGA 1140_H32
GCCTTTCG TACTG ACCTCCC GGGAGGACGAUGCGGGCCUU UCGUACUGACCUCCCCAGACGACUCGCCCGA 1141_H32
TCCTGGCGXCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGGCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1142_H32 0316. .H04
TCCGCACAACG TTCCTGCCC GGGAGGACGAUGCGGUCCGCACAACGUUCCUGCCCCAGACGACUCGCCCGA 1143_H32
TTTGAAGGGGGCTGGGTCCG GGGAGGACGAUGCGGUU UGAAGGGGGCUGGGUCCGCAGACGACUCGCCCGA 1144_H32 2282. .H04
TTTG AAG G G ATATG GGTCCG GGGAGGACGAUGCGGLi U UGAAGGGAUAUGGGUCCGCAGACGACUCGCCCGA 1145„H32 3072. .H04
TT'TGAGAGGTGATGGGTCCG GGGAGGACGAUGCGGUU UGAGAGGUGAUGGG UCCGCAGACGACUCGCCCGA 1146„H32
TTGGAAGGGGGGTGGGACCG GGGAGGACGAUGCGGUUGGAAGGGGGGUGGGACCGCAGACGACUCGCCCGA 1147 H32
# in m
UTS read variable region SNA sequences ft in H3 selection selection Selected sequenc
TIG AAG G G G ATG G GTCCG GGGAGGACGAUGCGGU UGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1148. .H32 2441 H04
TTGGAAGGAGGGTGGGACCG GGGAGGACGAUGCGGU UGGAAGGAGGG UGGGACCGCAGACGACUCGCCCGA 1149. _H32
TATTGGAGGTGTAAGGACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAAGGACCGCAGACGACUCGCCCGA 1150. .H32
CCCTTTCGTTCTCACCTCCC GGGAGGACGAUGCGGCCCUU UCGUUCUCACCUCCCCAGACGACUCGCCCGA 1151. _H32
TTTGAAAGGTGGTGGGTCCG GGGAGGACGAUGCGGU UUGAAAGGUGG UGGGUCCGCAGACGACUCGCCCGA 1152. .H32 4764_HG4
GTTGGGTGTAGGGAGTTCCG GGGAGGACGAUGCGGGUUGGGUGUAGGGAGUUCCGCAGACGACUCGCCCGA 1153. _H32
TTTGAATGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAUGGUGG UGGGUCCGCAGACGACUCGCCCGA 1154. .H32 3760_H04
TTGGAAGTAGG GTG GTACCG GGGAGGACGAUGCGGUUGGAAGUAGGGUGGUACCGCAGACGACUCGCCCGA 1155. .H32 4244_H04
GTTGTAGG GTG ATG G GTCCG GGGAGGACGAUGCGGGUUGUAGGG UGAUGGGUCCGCAGACGACUCGCCCGA 1156. .H32 4333 H04
TGTAGGAGGAGTAG GTACCG GGGAGGACGAUGCGGUGUAGGAGGAGUAGG UACCGCAGACGACUCGCCCGA 1157. .H32
ATTGGTGAACGGAGGTACTG GGGAGGACGAUGCG6AUUGGUGAACGGAGGUACUGCAGACGACUCGCCCGA 1158. .H32
AGAGCGAGAATCTGGTGCAC GGGA6GACGAUGCGGAGAGCGAGAAUCUGGUGCACCAGACGACUCGCCCGA 1159. _H32
AATAGTCCTTG CCTTG TGCC G GG AGG ACG AU G CG GAA UAGUCCUUGCCUUG U GCCC AG ACGACU CG CCCG A 1160_ _H32
o
TG ATCTTCTG G GT ACCACCC GGGAGGACGAUGCGGUGAUCUUCUGGGUACCACCCCAGACGACUCGCCCGA 1161. .H32
TGT AG TG G GTAC GTCG T6TG GGGAGGACGAUGCGGUGUAGUGGGUACGUCG UGUGCAGACGACUCGCCCGA 1162. „H32
CACCTTATCTTTG GTG ACCC GGGAGGACGAUGCGGCACCUUAUCUUUGG UGACCCCAGACGACUCGCCCGA 1163. _H32
ACG ACAGTCCTCTG CG CGTG GGGAGGACGAUGCGGACGACAGUCCUCU6CGCG UGCAGACGACUCGCCCGA 1164. _H32
TGTGATCGTCTGTTCCTCCA GGGAGGACGAU6CGGUGUGAUCGUCUGU UCCUCCACAGACGACUCGCCCGA 1165. „H32
TTAGA6TATGTTGGGTGCTG GGGAGGACGAUGCGGU UAGAG UAUG UUGGGUGCUGCAGACGACUCGCCCGA 1166. „H32
TTCG GGCCATCCTG CGTG TG GG6AGGACGAUGCGGU UCGGGCCAUCCUGCGUGUGCAGACGACUCGCCCGA 1167. .H32
CTCTGATGGAGGAGGGTGGA GGGAGGACGAUGCGGCUCUGAUGGAGGAGGG UGGACAGACGACUCGCCCGA 1168. HB2
TGAGTTCGTACTGTCGGCCC GGGAGGACGAUGCGGUGAG UUCGUACUGUCGGCCCCAGACGACUCGCCCGA 1169. .H32
TCGTTCCTGTCGTCGTGGTG GGGAGGACGAUGCGGUCGUUCCUGUCGUCGUGGUGCAGACGACUCGCCCGA 1170. .H32
CATCAGTATACTCCCCACTG GGGAGGACGAUGCGGCAUCAGUAUACUCCCCACUGCAGACGACUCGCCCGA 1171. _H32
TCG6TCATGTCGTTATGCTG GGGAGGACGAUGCGGUCCGOCAUGUCGUUAUGCUGCAGACGACUCGCCCGA 1172. _H32
TCCCTCTGTCGT6TGCTGTC GGGAGGACGAUGCGGUCCCUCUGUCGUGU6CUGIJCCAGACGACUCGCCCGA 1173. .H32
AACAGTCACTGGCTTGCCGC GGGAGGACGAUGCGGAACAG UCACUGGCUUGCCGCCAGACGACUCGCCCGA 1174. _H32
CAGAAGGCCAACTGCATGT6 GGGAGGACGAUGCGGCAGAAGGCCAACUGCAUG UGCAGACGACUCGCCCGA 1175 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
GTTAGTCTATGCGCGGTGCC GGGAGGACGAUGCGGG UUAGUCUAUGCGCGGUGCCCAGACGACUCGCCCGA 1176_H32
GACTGTCCTTCGTCGTGGTG GGGAGGACGAUGCG6GACUGUCCUUCGUCGUGGUGCAGACGACUCGCCCGA 1177_H32
TCCTTCCGTCGTTTGTGCCC GGGAGGACGAUGCGGUCCUUCCGUCGUU UGUGCCCCAGACGACUCGCCCGA Π78_Η32
TTG GGCGTAGG GTG GTACTG GGGAGGACGAUGCGGU UGGGCGUAGGGUGG UACUGCAGACGACUCGCCCGA 1179_H32
TGCGACACTATTTTGGCTG GGGAGGACGAUGCGGUGCGACACUAUUUUGGCUGCAGACGACUCGCCCGA 11S0_H32
TTTCGTCTCCCCTACGTGCC GGGAGGACGAUGCGGU UUCGUCUCCCCUACGUGCCCAGACGACUCGCCCGA 1181JH32
ACCGACACGTCTTCCATGGC GGGAGGACGAUGCGGACCGACACGUCUUCCAUGGCCAGACGACUCGCCCGA 1182_H32
TCTAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUCUAGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 1183JT32
TCAC AG ATCCTCCTTG TG G C GGGAGGACGAUGCGGUCACAGAUCCUCCUUGUGGCCAGACGACUCGCCCGA 1184_H32
TTTGAAGGGAGACGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGAGACGGG UCCGCAGACGACUCGCCCGA 1185_H32
TAG GTC G CTGCG CCGTTGTG GGGAGGACGAUGCGGUAGG UCGCUGCGCCGUUGUGCAGACGACUCGCCCGA 1186_H32
AGGCCCATGTCGTCGTGTG GGGAGGACGAUGCGGAGGCCCAUGUCGUCG UGUGCAGACGACUCGCCCGA 1187_H32
TTG GAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUGGAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 1188_H32
TTGGGAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUGGGAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1189„H32
TCCTGTCGTCTGTTGGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUGGUCCCCAGACGACUCGCCCGA 1190_H32 0154, .H04
AACCTCATCTTAACGTGCCC GGGAGGACGAUGCGGAACCUCAUCUUAACGUGCCCCAGACGACUCGCCCGA 1191_ H32
TATCCCAAAATGCCCCACCG GGGAGGACGAUGCGGUAUCC.CAAAAUGCCCCACCGCAGACGACUCGCCCGA 1192_H32
TGTC6AACGGTGTTTATCGC GGGAGGACGAUGCGGUGUCGAACGGUGUUUAUCGCCAGACGACUCGCCCGA 1193._H32
TTTG AAGG GAG AAG GGTCCG SGGAGG ACG A UG CG G U U UG AA G GG A G AAGG G U CCGCAG ACGAC U CG CCCG A 1194_H32 3-279. _HQ4
TCTTCTCGGCGCTCGTTGTG GGGA6GACGAUGCGGUCUUCUCGGCGCUCGUUGUGCAGACGACUCGCCC6A 1195JH32
TTTG AA TG G GG G TG T A GG GT GGGAGGACGAUGCGGUUUGAAUGGGGGUGUAGGGUCAGACGACUCGCCCGA 1196_H32
TTTG A AGG GTG A AG GG ACCG GGGAGGACGAUGCGGUUUGAAGGGUGAAGGGACCGCAGACGACUCGCCCGA 1197JH32
TTTGGGGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUGGGGGGUGAUGGGUCCGCAGACGACUCGCCCGA 1198_H32 3100. ,HQ4
TATTG GAG GTG TATG G ACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAUGGACCGCAGACGACUCGCCCGA I199_H32
TGTAGGAGGAGGAGGTACCT GGGA6GACGAUGCG6UGUAGGAG6AGGAGGUACCUCAGACGACUCGCCCGA 1200_H32
GTCCTGTCGTCTTTCGTCCC GGGAGGACGAUGCGGGUCCUGUCGUCUUUCGUCCCCAGACGACUCGCCCGA 1201_H32 2650. .HQ4
ATTG G TG A AG G G ATG AC CG GGGAGGACGAUGCGGAU UGGUGAAGGGAUGUACCGCAGACGACUCGCCCGA 1202_H32
TTTGAAGGGAGATGGATCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGGAUCCGCAGACGACUCGCCCGA 1203JH32 4161. .H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AGTGAGCTCGTCTATGGCCC GGGAGGACGAUGCGGAGUGAGCUCGUCUAUGGCCCCAGACGACUCGCCCGA 1204JH32
ATTGGAGGAGGGTGGGACTG GGGAGGACGAUGCGGAUUGGAGGAGGGUGGGACUGCAGACGACUCGCCCGA 1205JH32
TTTGAATGGGGATGGGTCCG GGGAGGACGAUGCGG UUUGAAUGGGGAUGGGUCCGCAGACGACUCGCCCGA 1206JH32 3352 H04
TTTGGAGTGGGGTGGGACCG GGGAGGACGAUGCGG UUUGGAGUGGG6UGGGACCGCAGACGACUCGCCC6A 1207_H32
TITGAAGGGTGCTGGGCCCG GGGAGGACGAUGCGGUUUGAAGGGUGCUGGGCCCGCAGACGACUCGCCCGA 120S_H32 4303 H04
TTGGGAGGAGGATGGTACCG GGGAGGACGAUGCGGUUGGGAGGAGGAUGGUACCGCA6ACGACUCGCCCGA 1209_K32
CCACGTTGTCTGCGACTGTG GGGAGGACGAUGCGGCCACG UUGUCUGCGACUGUGCAGACGACUCGCCCGA 1210 H32
TAGCTTTACCCTGCAAGCTA GGGAGGACGAUGCGGUAGCUUUACCCUGCAAGCUACAGACGACUCGCCCGA 1211_H32
CATAATACTCCTGTCGTGTA GGGAGGACGAUGCGGCAUAAUACUCCUGUCGUGUACAGACGACUCGCCCGA 1212_H32
GTGGTGTCGATCTTCGTCCC GGGAGGACGAUGCGGG UGCUG UCGAUCUUCGUCCCCAGACGACUCGCCCGA 1213_ H32
CATCGTTTTCGTCGCGTTAC GGGAGGACGAUGCGGCAUCGUUUUCGUCGCGUUACCAGACGACUCGCCCGA 1214_H32
GCCTTTCGTTCGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCGCCCUCCCCAGACGACUCGCCCGA 1215.JH32 2179 H04
TCTTCACCTGCCGTCGTGTG GGGAGGACGAUGCGGUCU UCACCUGCCGUCGUGUGCAGACGACUCGCCCGA 1216_H32
TTT6AAGGGTGATGGCTCCC 6GGAGGACGAUGCGGUUUGAAGGGUGAUGGCUCCCCAGACGACUCGCCCGA 1217_H32 3157 H04
ATCGATCTAATCCCGGTCGC 6GGAGGACGAUGC6GAUCGAUCUAAUCCCGGUCGCCAGACGACUCGCCCGA 12i8_H32
TCCTGTTCCTCTCGTGTCGG GGGAGGACGAUGCGGUCCUGUUCCUCUCGUGUCGGCAGACGACUCGCCCGA 1219_H32
C ATT G TCTT ATG TCG TGTG C GGGAGGACGAUGCGGCAUUGUCUUAUGUCGUGUGCCAGACGACUCGCCCGA 1220JH32
TCCCCTGATTGTCGTTTGTG GGGAGGACGAUGCGG UCCCCUGAUUGUCGUUUGUGCAGACGACUCGCCCGA 1221_H32
GCCTTTCGTTCTCTCCTCCC GG6AGGAGGAUGCG6GCCUUUCGUUCUCUCCUCCCCAGACGACUCGCCCGA 1222_H32 1028 H04
TGTCCTTCATTAGGCCGCAC GGGAGGACGAUGCGGUGUCCUUCAUUAGGCCGCACCA6AC6ACUCGCCCGA 1223JH32
TCTGGTTCTCCTGCTCGCTG GGGAGGACGAUGCGGUCUGGUUCUCCUGCUCGCUGCAGACGACUCGCCCGA 1224JH32
AAAGTCGGCTCTGGTGTGCC GGGAGGACGAUGCGG,AAAGUCGGCUCUGGUGUGCCCAGACGACUCGCCCGA 1225_H32
ACACCATTACTT'CGCCACCC GGGAGGACGAUGCGGACACCAUUACUUCGCCACCCCAGACGACUCGCCCGA 1226_H32
TCCTGTGTCTCCACGTACTG GGGAGGACGAUGCGGUCCUGUGUCUCCACGUACUGCAGACGAGUCGCCCGA 1227_H32
TG AGTCGTAATTTG CCG CGT GGGAGGACGAUGCGGUGAGUCGUAAUUUGCCGCGUCAGACGACUCGCCCGA I228_H32
ATTG GTAG GG AGG TACTG GGGAGGACGAUGCGGAUUGGUAGGGAGG UACUGCAGACGACUCGCCCGA 1.229_H32
TGAGTACCTCCGTTCGTGTC GGGAGGACGAUGCGGUGAGUACCUCCGUUCGUGUCCAGACGACUCGCCCGA 1230_H32
TCCCCATGTGTCTTCTGTGC GGGAGGACGAUGCGGUCCCCAUG UGUCUUCUG UGCCAGACGACUCGCCCGA 1231 H32
HTS read variable region RNA sequences # in H3 se!ection selection Selected sequenc
TAAAGTGCGGGTTC6TGTC GGGAGGACGAUGCGG UAAAGUGCGGGUUCG UGUCCAGACGACUCGCCCGA 1232. _H32
TTAGTCGTGTTCTGTCGCTG GGGAGGACGAUGCGGUUAGUCGUGUUCUGUCGCUGCAGACGACUCGCCCGA 1233. _H32
CGCTTCCTCTGGTTCGTCGC GGGAGGACGAUGCGGCGCUUCCUCUGGUUCG UCCCCAGACGACUCGCCCGA 1234_ .H32 366G_ H04
TGAGTCTGACGACTGTGCGC GGGAGGACGAUGCGGUGAGUCUGACGACUGUGCGCCAGACGACUCGCCCGA 1235. _H32
ATTGACCTCTATCCCGTGCC GGGAGGACGAUGCGGAUUGACCUCUAUCCCGUGCCCAGACGACUCGCCCGA 1236. _H32
CCCGTCCTTCCTCGTrrGCC GGGAGGACGAUGCGGCCCGUCCUUCCUCGU UUGCCCAGACGACUCGCCCGA 1237_ .H32
TCTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGUCUGAAGGGCGAIJGGGUCCGCAGACGACUCGCXCGA 1238. _H32 1956_ H04
TTTGAAGGGTGATCTCCG GGGAGGACGAUGCGGU UUGAAGGG UGAUCUCCGCAGACGACUCGCCCGA 1239. _H32 2663_ H04
TTTGATGGGAGATGG6TCCG GGGAGGAGGAUGCGGUUUGAUGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1240_ H32 2984_ H04
TGTGTAGTCTATCCGCCCG GGGAGGACGAUGCGGUGUGUAGUCUAUCCGCCCGCAGACGACUCGCCCGA 1241. _H32 45S3_ H04
TTTGAAGTGGGATGGGACCG GGGAGGACGAUGCGGUUUGAAGUGGGAUGGGACCGCAGACGACUCGCCCGA 1242. .H32
GCCTTTCGTGCTGACCTCCC GGGAGGACGAUGCGGGCCU UUCGUGCUGACCUCCCCAGACGACUCGCCCGA 1243. .H32
CATTGTCTCCCGTCGGTGTG GGGAGGACGAUGCGGCAUUGUCUCCCGUCGGUGUGCAGACGACUCGCCCGA 1244. _H32
TCCTGTCGTCTGTACGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGIJACG UCCCCAGACGACUCGCCCGA 1245. _H32 0319_ H04
AT6GGTTGTCTTACCGGTGC GGGAGGACGAUGCGGAUGGGUUG UCUUACCGGUGCCAGACGACUCGCCCGA 1246. H32
GTCCTGTGTCTGTTCGTCCC GGGAGGACGAUGCGGGUCCUG UGUCUGU UCGuCCCCAGACGACUCGCCCGA 1247. .H32 3033„ .H04
TTTGAAGTGAGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGUGAGAUGGGUCCGCAGACGACUCGCCCGA 1248. .H32 3354. H04
TTTG AAG G G AAATG G GTCCG GGGAGGACGAUGCGGUUUGAAGGGAAAUGGGUCCGCAGACGACUCGCCCGA 1249. _H32 3222 . .HQ4
ATAGGGGGAAGGGTGAACCG GGGAGGACGAUGCGGAUAGGGGGAAGGGUGAACCGCAGACGACUCGCCCGA 1250. _H32
TGTATGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUAUGAGGAGGAGG UACCGCAGACGACUCGCCCGA 1251. .H32
TTGGGTGTAGG6TGGACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGACCGCAGACGAGUCGCCCGA 1252. .H32
GTGGGTGTAGGGTGTACCG GGGAGGACGAUGCGGGUGGGUGUAGGGUG UACCGCAGACGACUCGCCCGA 1253. .H32
TTTGAAGGGAGATGAGTCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGAGUCCGCAGACGACUCGCCCGA 1254. _H32 4597_ .HQ4
TTTG AAG G G CG ATG G ATCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGAUCC6CAGACGACUCGCCCGA 1255. _H32 2874_ .H0
TTGAAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1256. _H32 2465_ .H04
TTTG GAGGAGGGTGGGTCCG GGGAGGACGAUGCGGUUUGGAGGAGGGUGGGUCCGCAGACGACUCGCCCGA 1257. _H32
TGTAGGGAGGTAGTTATCGC GGGAGGACGAUGCGGUGUAGGGAGGUAGUUAUCGCCAGACGACUCGCCCGA 258. .H32
G ICG TTTGTTAAG TG G CCGC GGGAGGACGAUGCGGGUCG UU UGU UAAGUGGCCGCCAGACGACUCGCCCGA 1259. .H32
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TATCGTCAGTTCTCCT6CGC GGGACGACGAUGCGGUAUCGUCAGUUCUCCUGCGCCAGACGACUCGCCCGA 1260_ _H32
TGAGTTTGTTTAGCTGGCCC GGGAGGACGAUGCGGUGAGUU UGUUUAGCUGGCCCCAGACGACUCGCCCGA 1261_ H32
CTTTCGTCTCCTGTCGTGCA GGGAGGACGAUGCGGCUUUCG UCUCCUGUCGUGCACAGACGACUCGCCCGA 1262. H32
TAGTCCTATGTCGTATGCTG GGGAGGACGAUGCGGUAGUCCUAUGUCGUAUGCUGCAGACGACUCGCCCGA 1263. _H32
TATGGGAGTAGGG.TGGCACG GGGAGGACGAUGCGGUAUGGGAG UAGGGUGGCACGCAGACGACUCGCCCGA 1264. _H32
C6TTTCCTCTGGTTCGTCG C GGGAGGACGAUGCGGCGU UUCCUCUGGUUCGUCGCCAGACGACUCGCCCGA 1265_ _H32
GTTGGTTCGTCTCCGTGTA GGGAGGACGAUGCGGGUUGG UUCGUCUCCGUGUACAGACGACUCGCCCGA 1266. _H32
TCTTTCTCCTCCTG TCG CTG GGGAGGACGAUGCGGUCU UUCUCCUCCUGUCGCUGCAGACGACUCGCCCGA 1267. _H32
GAACAACTTACTTCCCTGGC GGGAGGACGAUGCGGG.AACAACUUACUUCCCUGGCCAGACGACUCGCCCGA 1268. _H32
TAGGTCTGGTGCAGTGTGTG GGGAGGACGAUGCGGUAGGUCUGGUGCAGUGUGUGCAGACGACUCGCCCGA 1269. .H32
CCGCATTGTTCGTCTGTGTC GGGAGGACGAUGCGGCCGCAUUGUUCGUCUG UGUCCAGACGACUCGCCCGA 1270. .H32
TGCGAACTCTGGGGTCTGTG GGGAGGACGAUGCGGUGCGAACUCUGGGGUCUGUGCAGACGACUCGCCCGA 1271. _H32
CACCCTTTATACTGCAAGGG GGGAGGACGAUGCGGCACCCUUUAUACUGCAAGGGCAGACGACUCGCCCGA 1272. _H32
TTGTGTAGGGTGGTACCG GGGAGGACGAUGCGGUUGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 1273. _H32
TACTTTATCCTGCAAGTGAC GGGAGGACGAUGCGGUACUUUAUCCUGCAAGUGACCAGACGACUCGCCCGA 1274. .H32
GCCTTTCGTTCTGCCTTCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCUUCCCAGACGACUCGCCCGA 1275. _H32 3404 H04
TAGAGTCG G ACTCTG GTCG C GGGAGGACGAUGCGGUAGAGUCGGACUCUGGUCGCCAGACGACUCGCCCGA 1276, .H32
CATTGTCCCGACATTATCCG 6GGAGGACGAUGCGGCAUUGUCCCGACAUUAUCCGCAGACGACUCGCCCGA 1277. ..H32
ATGGG TTCGCTG CAGTTGTG GGGAGGACGAUGCGGAUGGGU UCGCUGCAGU UGUGCAGACGACUCGCCCGA 1278. .H32
TTGG GTGTAAA G TGGTACCG GGGAGGACGAUGCGGUUGGGUGUAAAGUG6UACCGCAGACGACUCGCCCGA 1279. H32
ACGGGTTAACCTGGTTACGC GGGAGGACGAUGCGGACGG6U UAACCUGGUUACGCCAGACGACUCGCCCGA 1280. _H32
AGAGACTACGGATTCGCGTG GGGAGGACGAUGCGGAGAGACUACGGAUUCGCGUGCAGACGACUCGCCCGA 1281. .H32
GTGTAGTG AAT G TG TG G CCC GG6AGGACGAUGCGGGUGUAG UGAAUGUGUGGCCCCA6ACGACUCGCCCGA 1282. _H32
TGCGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUGCGAAGGGUGAUGG6UCCGCAGACGACUCGCCCGA 1283. _H32 1485 K04
TTAGTC CTATCGTTTG G CA C GGGAGGACGAUGCGGU UAGUCCUAUCGUUUGGCACCAGAC6ACUCGCCCGA 1284. _H32
TG AG G 'f AT6CTCTG CGTGTG GGGAGGACGAUGCGGUGAGGUAUGCUCUGCGUGUGCAGACGACUCGCCCGA 1285. _H32
ACATGTCGTTCGCCTTGGTG GGGAGGACGAUGCGGACAUGUCGUUCGCCUUGGUGCAGACGACUCGCCCGA 1286. _H32
GGCTTTCGGTTTGCCCTCCC GGGAGGACGAUGCGGGCCU UUCGGUUUGCCCUCCCCAGACGACUCGCCCGA 1287 H32 0949 H04
if in H4
HTS read variable region RNA sequences if in H3 selection selection Selected sequenc
GTCCTATCCTCTGTTCGTCCC GGGAGGACGAUGCGGG UCCUAUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 12SS_ ,H32 3801 H04
TCCCGTCGTTGCGATTTCGC GGGAGGACGAUGCGGUCCCG UCGUUGCGAUUUCGCCAGACGACUCGCCCGA 1289_ _H32
AAATTCGTCTCCCCGTTGTG GGGAGGACGAUGCGGAAAUUCGUCUCCCCG UUGUGCAGACGACUCGCCCGA 1290. .H32
ACTGTGTATAGACACTGGCC GGGAGGACGAUGCGGACUGUG UAUAGACACUGGCCCAGACGACUCGCCCGA 1291. H32
TATTG G AG GTG G A G GG ACCG GGGAGGACGAUGCGGUAUUGGAGGUGGAGGGACCGCAGACGACUCGCCCGA 1292_ .H32
ATTGGTGAAAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGUGAAAGGGAGGUACUGCA6ACGACUCGCCCGA 1293. .H32
ATTTGGTGAAGGGAGGTACTG GGGAGGACGAUGC6GAUUUGGUGAA6G6AGGUACUGCAGACGACUCGCCC6A 1294. ,H32
ATTGGTG AAGG GAG GTACCT GGGAGGACGAUGCGGAUUGGUGAAGGGAGG UACCUCAGACGACUCGCCCGA 1295_ _H32
TGAGTGTCTGCTTTCCACCC GGGAGGACGAUGCGGUGAGUG UCUGCUUUCCACCCCAGACGACUCGCCCGA 1296. H32
GTGGAGACTACATGTGGCGC GGGAGGACGAUGCGGGUGGAGACUACAUGUGGCGCCAGACGACUCGCCCGA 1297. .H32
TAATGCAGTGTTTCGTGCCC GGGAGGACGAUGCGGUAALiGCAGlJGUUUCG UGCCCCAGACGACUCGCCCGA 1298. _H32
TGTCACTTTTCACGTGGCTG G GG AGG ACGAUGCGGU G UCACU U U UCACG UG GCUGCAGACG ACUCG CCCGA 1299. _H32
TCCTTCACTGATTCG CTGTG GGGAGGACGAUGCGGUCCU UCACUGAUUCGCUGUGCAGACGACUCGCCCGA 1300_ .H32
AAGCCTCCGTGTTTGCGCGC GGGAGGACGAUGCGGAAGCCUCCGUG UUUGCGCGCCAGACGACUCGCCCGA 1301_ _H32
TTTGAAGTGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGUGUGGUGGGUCCGCAGACGACUCGCCCGA 1302. .H32
TTTGAAGGGGGATGGGTCCC GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGUCCCCAGACGACUCGCCCGA 1303. .H32 1519 H04
TGTAGGGAGGCAGTTATCGT GGGAGGACGAUGCGGUGUAGGGAGGCAGUUAUCGUCAGACGACUCGCCCGA 1304. .H32
TTGGGTGGAGGGGGGTACCG GGGAGGACGAUGCGGU UGGGUGGAGGGGGGUACCGCAGACGACUCGCCCGA 1305. .H32
GCCTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCCUCGUUCUGACCUCCCCAGACGACUCGCCCGA 1306. .H32
AACCGAGGTCCTTCGTACCC GGGAGGACGAUGCGGAACCGAGGUCCUUCGUACCCCAGACGACUCGCCCGA 1307_ .H32
TCCGCGGTTTGTCACCTCAC GGGAGGACGAUGCGGUCCGCGG UUU6UCACCUCACCAGACGACUCGCCCGA 1308_ H32
GTGACTTCCCTGGTTCTC6C GGGAGGACGAUGCGGGUGACUUCCCUGG UUCUCGCCAGACGACUCGCCCGA 1309. „Η32
TGTCCAATGAATGTCTGCCC GGGAGGACGAUGCGGUGUCCAAUGAAUGUCUGCCCCAGACGACUCGCCCGA 1310. .H32
ATTG A AGG G G G ATG GG ACCG GGGAGGACGAUGCGGAUUGAAGGGGGAUGGGACCGCAGACGACUCGCCCGA 1311. .H32
TTTGAAGGGTTGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUUGUGGGUCCGCAGACGACUCGCCCGA 1312. .H32 2955 H04
TTTG G AG G GGG ATG GGTCTG GGGAGGACGAUGCGGUUUGGAGGGGGAUGGGUCUGCA6ACGACUCGCCCGA 1313. .H32
TTTGGAGAAGGGAGGTACTG GGGAGGACGAUGCGGUUUGGAGAAGGGAGGUACUGCAGACGACUCGCCCGA 1314. H32
TTTTAAGGGTGGTGGGTCCG GGGAGGACGAUGCGGU UUUAAGGGUGGUGGGUCCGCAGACGACUCGCCCGA 1315 H32 4965 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTGGGAGGSGG6TGGTACCG GGGAGGACGAUGCGG U UGGGAGGGGGGUGGUACCGCAGACGACUCGCCCGA 1315, .H32
TGTAGGA6GATGA6GTACCG GGGAGGACGAUGCGGUGUAGGAGGAUGAGGUACCGCAGACGACUCGCCCGA 1317. H32
TTTGAAGAGGGATGGGACCG GGGAGGACG AUGCGG U UUGAA6AGGGAUGGGACCGCAGACGACUCGCCCGA 1318. _H32
TTTGAAGGGAG ATG G GTACG GGGAGGACGAUGCGGU UUGAA6GGAGAUGGGUACGCAGAC6ACUCGCCCGA 1319_ H32 3632 H04
ACAGGTCGTTCTGACTCCAC GGGAGGACGAUGCGGACAGG UCGUUCUGACUCCACCAGACGACUCGCCCGA 1320_ _H32
TCGAGTCCTCATCTGTGCTG GGGAGGACGAUGCGGUCGAGUCCUCAUCUGUGCUGCAGACGACUCGCCCGA 1321_ .H32
G TCTTCTG TCTTATCGTC CC G6GAGGACGAUGCGGGUCUUCUGUCUUAUCGUCCCCAGACGACUC6CCCGA 132.2^ _H32
CATCGATCCGT TACGTGTG GGGAGGACGAUGCGGCAUCGAUCCGUCUACG UGUGCAGACGACUCGCCCGA 1323. H32
TTAG TGTACAATCCCTG CCC GGGAGGACGAUGCGGUUAGUGUACAAUCCCUGCCCCAGACGACUCGCCCGA 1324. .H32
GTAGACGTCCGCGCGTTGTG GGGAGGACGAUGCGGG UAGACG UCCGCGCGUUGUGCAGACGACUCGCCCGA 1325. .H32
TCTCTCTCGTCGTCACTGTG GGGAGGACGAUGCGGUCUCUCUCGUCGUCACUGUGCAGACGACUCGCCCGA 1326. .H32
GTTGACTCGTCTCCTCTCCC GGGAGGACGAUGCGGG UUGACUCGUCUCCUCUCCCCAGACGACUC.GCCC6A 1327. _H32
GATCGTACTTTCCCCG TGCC GGGAGGACGAUGCGGGAUCGUACUUUCCCCGUGCtCAGACGACUCGCCCGA 1328. .H32
ATCCACATCCTCTTTGTCCG GGGAGGACG AUGCGGAUCCACAUCCUCUUUGUCCGCAGACGACUCGCCCG A 1329. .H32
TTTTCCTTTGTCGCTGGCGC GGGAGGACGAUGCGGUUUUCCU UUGUCGCUGGCGCCAGACGACUCGCCCGA 1330. .H32
TTCCTCAAATGTCGTGTGTG GGGAGGACGAUGCGGUUCCUCAAAUGUCGUGUGUGCAGACGACUCGCCCGA 1331.. _H32
TGCCGTTAGATCGCCTACCC GGGAGGACGAUGCGGUGCCG UUAGAUCGCCUACCCCAGACGACUCGCCCGA 1332. .H32
CACCCTTC TG TTGCCTG CC GGGAGGACGAUGCGGCACCCU UCAUGUUGCCUGCCCAGACGACUC6CCCGA 1333. .M32
TGATTCGTCTCATACGTGGC GGGA6GACGAUGCGGUGAUUCGUCUCAUACGUGGCCAGACGACUCGCCCGA 1334_ .H32
ACAGAAGTCGTCATTGCCCC GGGAGGACGAUGCGGACAGAAGUCGUCAUUGCCCCCAGAC ACUr.GCCCGA 1335. .H32
AAGGCCCTCGTCGTCTGCTG GGGAGGACGAUGC6GAAGGCCCUCGUCG UCUGCUGCAGACGACUCGCCCGA 1336. .H32
CTAGTTCCTTCTCGTGTGGC GGGAGGACGAUGCGGCUAGUUCCUUCUCGUGUGGCCAGACGACUCGCCCGA 1337_ _H32
CAAAGTCATCCTCGCGTGTG GGGAGGACGAUGCGGCAAAGUCAUCCUCGCG UGUGCAGACGACUCGCCCGA 1338. „H 2
GGCCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGGCCUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 1339. .H32 2691 H04
CTGGGTGTAGGGTGGTACTG GGGAGGACGAUGCGGCUGGGUGUAGGGUGG UACUGCAGACGACUCGCCCGA 1340. .H32
TCGCTCGTTATGTCGTTGCA GGGAGGACGAUGCGG UCGCUCGU UAUGUCGUUGCACAGACGACUCGCCCGA 1341. .H32
TAG GTAGTG G G ACTGTG CCC GGGAGGACG AUGCGG UAGG UAG UGGGACUG UGCCCCAGACGACUCGCCCG A 1342. H32
GTCTTGG CTTATCGTCCC GGGAGGACGAUGCGGGUCUUGGCUUAUCGUCCCCAGACGACUCGCCCGA 1343 H32
# in H4
NTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CAATG ATG TCCTCTCTCCCC GGGAGGACGAUGCGGCAAUGAUGUCCUCUCUCCCCCAGACGACUCGCCCGA 1344_H32
CCG CGGAATTGTCCCCTGTG GGGAGGACGAUGCGGCCGCGGAAUUGUCCCCUG UGCAGACGACUCGCCCGA 1345_H32
TCACTCACAATCCTTCACCG GGGAGGACGAUGCGGUCACUCACAAUCCtlUCACCGCAGACGACUCGCCCGA 1346_H32
ATCCTCTGGTCATGTTGCGC GGGAGGACGAUGCGGAUCCUCUGG UCAUG UUGCGCCAGACGACUCGCCCGA 1347„H32
TTTGAATGGCGATGGGTCCG GGGAGGACGAUGCGGUUUGAAUGGCGAUGGGUCCGCAGACGACUCGCCCGA 1348_H32 2979 H04
GCTGC AGA ACATG TG G ACCC GGGAGGACGAUGCGGGCUGCAGAACAUGUGGACCCCAGACGACUCGCCCGA 1349_H32
TAATGTCTAGTCCCGGACCC GGGAGGACGAUGCGGUAAUGUCUAGUCCCGGACCCCAGACGACUCGCCCGA 135G_H32
GATTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGGAUUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 1351_H32
ATTTTTTGGAGAAGGGAGGTG GGGAGGACGAUGCGGAUUUUUUGGAGAAGGGAGGUGCAGACGACUCGCCCGA ±352_H32
ACGGTGTCGTGAGCTCTGTG GGGAGGACGAUGCGGACGGUGUCGUGAGCUCUGUGCAGACGACUCGCCCGA 1353._H32
TTTG G AG AAGG G TG G G ACCG GGGAGGACGAUGCGGU UUGGAGAAGG6UGGGACCGCAGACGACUCGCCCGA 1354_H32
TGTGGTCTGGTTAGCTCGGC GGGAGGACGAUGCGGUGUGGUCUGGUUAGCUCGGCCAGACGACUCGCCCGA 1355_H32
TTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGU UGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 1356„H32 2959_H04
ACAAAAACCTCCCACGTGCC GGGAGGACGAUGCGGACAAAAACCUCCCACGUGCCCAGACGACUCGCCCGA 1357_H32
AGTCTGGTCAAGCCGTTGTG GGGAGGACGAUGCGGAGUCUGGUCAAGCCG UUGUGCAGACGACUCGCCCGA 13S8_H32
AACCGCGTGACTGTATGCCC GGGAG6ACGAUGCGGAACCGCGUGACUGUAUGCCCCAGACGACUCGCCCGA 1359_H32
NTGGGGTTTTCTCGTGCCC GGGAGGACGAUGCGGUUUGGGGUUUUCUCGUGCCCCAGACGACUCGCCC6A 136G_H32
TCCTGGATTGTCGTTGTCCC GGGAGGACGAUGCGGUCCUGGAUUGUCGUUGUCCCCAGACGACUCGCCCGA 1361_H32
GCCTTCGTTTGTTCGTCCC GGGAGGACGAUGCGGGCCUUCGUUUGUUCGUCCCCAGACGACUCGCCCGA 1362_H32
AATTGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGAAUUGG UGAAGGGAGGUACUGCAGACGACUCGCCCGA 1363_H32
TGGTG AAG GG AGGTACTG GGGAGGACGAU6CGGUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 1364_H32
TTTGACGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGACGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1365_H32 3133_H04
GCCTTTCGTTCTGACCCTCCC GGGAGGACGAUGCGGGCCUU UCGUUCUGACCCUCCCCAGACGACUCGCCCGA 1366_H32 3S54 H04
ACCGTCAAACGTCTGTGCGC GGGAGGACGAUGCGGACCGUCAAACGUCUGUGCGCCAGACGACUCGCCCGA 1367_H32
TTCCG CGTCCGTTTCCTG TG GGGAGGACGAUGCGGU UCCGCGUCCG UUUCCUG UGCAGACGACUCGCCCGA 1368_ H32
ATTGGTGAAG G GGTACTG GGGAGGACGAUGCGGAU UGGUGAAGGGGUACUGCAGACGACUCGCCCGA 1369 .H32
CGATATCGTCGTGTTGGTGC GGGAGGACGAUGCG6CGAUAUCGUCGUGUUGGUGCCAGACGACUCGCCCGA 1370_H32
TCAGTTGTCTTG GTG CGCAC GGGAGGACGAUGCGGUCAGUUG UCUUGGUGCGCACCAGACGACUCGCCCGA 1371 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AGCGATGTTTGTTGTGTG6C GGGAGGACGAUGCGGAGCGAUGUUUGUUGUGUGGCCAGACGACUGGCCCGA 1372. _H32
TCCTGGCTTGTCGTTTCCC GGGAGGACGAUGCGGUCCUGGCUUGUCGUUUCCCCAGACGACUCGCCCGA 1373. _H32
TCTGTAGATAACGTCTGCCC GGGAGGACGAUGCGGUCUGUAGAUAACGUCUGCCCCAGACGACUCGCCCGA 1374 . „H32
AATCGGGTTATCGTCTTGGC GGGAGGACGAUGCGGAAUCGGGUUAUCGUCUUGGCCAGACGACUCGCCCGA 1375, „H32
TTG G GTGTAGGGTGGTAT6 GGGAGGACGAUGCGGUUGGG UGUAGGGUGGUAUGCAGACGACUCGCCCGA 1376. _H32
TTTGAACGGCGATGGGTCCG GGGAGGACGAUGCGGUUUGAACGGCGAUGGGUCCGCA6ACGACUCGCCCGA 1377, _H32 1907. _H04
GTTGAGTCTGCGTTGCTGCC GGGAGGACGAUGCGGGUUGA6UCUGCGUUGCLGCCCAGACGACUCGCCCGA 1378. _H32
CATTTTCTTCCTG TCGTG TG GGGAGGACGAUGCGGCAUUUUCUUCCUGUCGUG UGCAGACGACUCGCCCGA 1379. _H32
AGTAGGTGTCGTCTTGTGGC GGGAGGACGAUGCGGAGUAGGUG UCGUCUUGUGGCCAGACGACUCGCCCGA 1380. _H32
TCCCTGTCGTCTTG CG TCCC GGGAGGACGAUGCGGUCCCUGUCGUCUUGCGUCCCCAGACGACUCGCCCGA 1381. _H32 2028_ ,H04
TTG G GTGTAG G GCG GTG CCG GGGAGGACGAUGCGGUUGGGUGUAGGGCGGUGCCGCAGACGACUCGCCCGA 1382. _H32
G TCCCGTCGTCTG TTCGTCCC GGGAGGACGAUGCGGGUCCCGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1383. _H32 3031. H04
CCA ATTTCTTC CCCG TG CTG GGGAGGACGAUGCGGCCAAU UUCUUCCCCGUGCUGCAGACGACUCGCCCGA 1384. .H32
TTCGAAGGGAGATGGGTCCG GGGAGGACGAUGCGGUUCGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 138.5. .H32 3'940_ H04
TTTGAAGGGGGATGGCTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGCUCCGCAGACGACUCGCCCGA 1386_ H32 3851„ H04
TTTTGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUUUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 1387. _H32
TTTGAAGTAGGGTGGGACCG GGGAGGACGAUGCGGUUUGAAGUAGGG UGGGACCGCAGACGACUCGCCCGA 1388. .H32
TTGGGAGTAG6GTGGCACCG GGGAGGACGAUGCGGUUGGGAGUAGGGUGGCACCGCAGACGACUCGCCCGA 1389. _H32
ATTGGTAAGG G AG G TACCG GGGAGGACGAUGCGGAUUGGUAAGGGAGGUACCGCAGACGACUCGCCCGA 1390. „H32
TTGGGGGTAGGGTGTTACCG GGGAGGACGAUGCGGUUGGGGGUAGGGUG UUACCGCAGACGACUCGCCCGA 1391. _H32
ATTGGAGGGGGGTGGGACTG GGGAGGACGAUGCGGAUUGGAGGGGGGUG6GACUGCAGACGACUCGCCCGA 1392. .H32
TTTGAAGGGAGATGGGTCAG GGGAGGACGAUGCGGUUUGAAGGGAGAUGGGUCAGCAGACGACUCGCCCGA 1393. .H32 3140. H04
TTTG A AG G G CGATG G CTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGCUCCGCAGACGACUCGCCCGA 1394. _H32 48Q5_ _HQ4
TTTGTAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGUAGGGGGAUGGGUCCGCA.GACGACUCGCCCGA 1395. .H32 2596_ .H04
TTTGATGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAUGGGUGGUGGGUCCGCAGACGACUCGCCCGA 1396. .H32 4183_ _H04
TTTGAAGGAGGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGAGGG UGGG UCCGCAGACGACUCGCCCGA 1397_ _H32
TTTGGAGAGGGGTGGGACCG GGGAGGACGAUGCGGtJUUGGAGAGGGGUGGGACCGCAGACGACUCGCCCGA 1398. _H32
TTTGGAGTAGGATGGGACCG GGGAGGACGAUGCGGUUUGGAGUAGGAUGGGACCGCAGACGACUCGCCCGA 1399 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ί TTG TAGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGUAGGG UGGUGGG UCCGCAGACGACUCGCCCGA 1400. .H32
ATAGGGAGAAGGGCGAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGCGAACCGCAGACGACUCGCCCGA 1401. _H32
TTTG A AG G G AG ATGTGTCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGUGUCCGCAGACGACUCGCCCGA 1402_ _H32 3487 H04
CCAACGTTCTCTCACGTGTG GGGAGGACGAUGCGGCCAACGUUCUCUCACGUGUGCAGACGACUCGCCCGA 1403. _H32
GATGTCTAG GG ATTTGTCCC GGGAGGACGAUGCGGGAUG UCUAGGGAUUUGUCCCCAGACGACUCGCCCGA 1404_ .H32
TTT6GAGTTTTCCCGTGCCC GGGAGGACGAUGCGGUUUGGAGUUUUCCCGUGCCCCAGACGACUCGCCCGA 1405_ .H32
TCTTCTTCTCCCATGTCGC GGGAGGACGAUGCGGUCUUCU UCUCCCAUGUCGCCAGACGACUCGCCCGA 1406. _H32
TC AC CTATG CTAGG GTG CCA G6GAGGACGAUGCGGUCACCUAUGCUAGGGUGCCACAGACGACUCGCCCGA 1407_ _H32
GCGTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCGUUUCGUUCUGACCUCCCCAGACGACUCGCCCGA 1408. .H32
TTCCTCTGTATTGCGCACCC GGGAGGACGAUGCGGUUCCUCUGUAUUGCGCACCCCAGACGACUCGCCCGA 1409, .H32
TTGGGTGTGGGGTGGTACTG GGGA6GACGAUGCGGUUGGGUGUGGGGUGGUACUGCAGACGACUCGCCCGA 1410. H32
GTAACGCGTCTG C ACG TGTC GGGAGGACGAUGCGGGUAACGCGUCUGCACGUGUCCAGACGACUCGCCCGA 1411. _H32
AGAGTCGTGCTGATTCCCCC GGGAGGACGAUGCGGAGAGI ICGUGCLIGAUUCCCCCCAGACGACUCGCCCGA 1412. .H¾
AG CG AGTATGTTTAGTG CTG GGGAGGACGAUGCGGAGCGAGUAUGUUUAGUGCUGCAGACGACUCGCCCGA 1413. .H32
ATCGGTCCACTTTGCGTGCC GGGAGGACGAUGCGGAUCGG UCCACU UUGCGUGCCCAGACGACUCGCCCGA 1414. .H32
GCTTGTCGTTCTCCTGTCTG GGGAGGACGAUGCGGGCUUGUCGUUCUCCUGUCUGCAGACGACUCGCCCGA 1415.. _H32
TTTG GGGTGATG6GTCCG GGGAGGACGAU6CGGUUUGGGGU6AUGGGUCCGCAGACGACUCGCCCGA 1416. .H32
AATGTCCTTTGCGGTCTCGC GGGAGGACGAUGCGGAAUGUCCUUUGCGG UCUCGCCAGACGACUCGCCCGA 1417. .H32
CCGCACGGTTATTCCCTCCG GGGAGGACGAUGCGGCCGCACGGUUAU UCCCUCCGCAGACGACiJCGCCCGA 1418. .H32
CAAGTGGTCTTACG GTCC ACCC GGGAGGACGAUGCGGCAAGUGG UCUUACGGUCCACCCC'AGACGACUCGCCCGA 1419. .H32
ATAGTCGAGCCTCTGTGCGC GGGAGGACGAUGCGGAUAGUCGAGCCUCUG UGCGCCAGACGACUCGCCCGA 1420. H32
TCCTTCGGTCTG CTCATGCC GGGAGGACGAUGCGGUCCUUCGGUCUGCUCAUGCCCAGACGACUCGCCCGA 1421. .H32
CGTATCGACTTGCCCCTGTG GGGAGGACGAUGCGGCGUAUCGACUUGCCCCUGUGCAGACGACUCGCCCGA 1422. _N32
CCTGTCGTTTGTACTGTTGC GGGAGGACGAUGCGGCCUGUCGU UUGUACUGUUGCCAGACGACUCGCCCGA 1423. .H32
TTAGTCG ATTG CCATGTGTG GGGAGGACGAUGCGGUUAGUCGAUUGCCAUGUGUGCAGACGACUCGCCCG 1424. .H32
TAGAGTCGTAACCGTGTCCC G6GAGGACGAUGCGGUAGAGUCGUAACCGUGUCCCCAGACGACUCGCCCGA 1425. _H32
TCCAACACCTGATGTCGCTG GGGAGGACGAUGCGGUCCAACACCUGAUGUCGCUGCAGACGACUCGCCCGA 1426. .H32
AG G CTAG CTG TCTG GTTGTG GGGAGGACGAUGCGGAGGCUAGCUGUCUGGUUGUGCAGACGACUCGCCCGA 1427 H32
# in H4
HTS read variable region RNA sequences tf in H3 selection selection Selected sequenc
TGAGAATGGATCTGGTGGGC GGGAGGACGAUGCGG UGAGAAUGGAUCUGGUGGGCCAGACGACUC6CCCGA 1428JH32
GCGGATAAATGGCCTGGGTA GGGAGGACGAUGCGGGCGGAUAAAUGGCCUGGG UACAGACGACUCGCCCGA 1429_H32
AUG GIG A AG GAG GTACCG GGGAGGACGAUGCGGAUUGGUGAAGGAGGUACCGCAGACGACUCGCCCGA 1430_H32
GTGACTTTATGAGTTGTGTG GGGAGGACGAUGCGGG UGACUUUAUGAGUUGUGUGCAGACGACUCGCCCGA 1431_H32
GATGACGGCGTTGTCGCTAC GGGAGGACGAUGCGGGAUGACGGCGU UGUC6CUACCAGACGACUCGCCCGA 1432...H32
GT6 ATCAG T'CCTCTGG TCTG GGGAGGACGAUGC6GGUGAUCAGUCCUCUGGUCUGCAGACGACUCGCCCGA 1433_H32
TTAGTCGAAGGTGTGCGTGC GGGAGGACGAUGCGGUUAGUCGAAGGUGUGCGUGCCAGACGACUCGCCCGA 1434_H32
TATGTAGTCGTCGTGTG CGC GGGAGGACGAUGCGGUAUGUAGUCGUCGUGUGCGCCAGACGACUCGCCCGA 1435_H32
CAGAGGAGGTGGTGACACCG GGGAGGACGAUGCGGCAGAGGAGGUGGUGACACCGCAGACGACUCGCCCGA 1436_H32
CTTGTCGTCTCCTTTCACCC GGGAGGACGAUGCGGCUUGUCGUCUCCUUUCACCCCAGACGACUCGCCCGA 1437_H32
G CCTGTCG TTCTG ACCTCCC GGGAGGACGAUGCGGGCCUGUCGUUCUGACCUCCCCAGACGACUCGCCCGA 1438_H32
ATCCTGCCTTGTCGTTGGCC GGGAGGACGAUGCGGAUCCUGCCUUGUCG UUGGCCCAGACGACUCGCCCGA 1439_H32
TTTG AAG G GTGTAG G GACCG GGGAGGACGAUGCGGUUUGAAGGGUGUAGGGACCGCAGACGACUCGCCCGA 1440_H32
o
o CCGTAGCGTCTCCATCTGTG GGGAGGACGAUGCGGCCGUAGCGUCUCCAUCUG UGCAGACGACUCGCCCGA 14 1_H32
CTAGTCCGTTTGTAACGCCA GGGAGGACGAUGCGGCUAGUCCGUU UGUAACGCCACAGACGACUCGCCCGA 1442_H32
TGAGCGCTCTGTTTCCGCCC GGGAGGACGAUGCGGUGAGCGCUCUGUUUCCGCCCCAGACGACUCGCCCGA 1443__H32
TG GG ACTCTGCG TGTTG TG GGGAGGACGAUGCGGUGGGACUCUGCGUGUUGUGCAGACGACUCGCCCGA 1444^H32
CTAATAGGTGGAGTAGGGTA GGGAGGACGAUGCGGCUAAUAGGUGGAGUAGGG UACAGACGACUCGCCCGA 1445__ H32
TTTGAAGGGTGATGGGTCGC GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGUCGCCAGACGACUCGCCCGA 1446_H32 3797 H04
TACTACAAGATAC AGTG CCC GGGAGGACGAUGCGGUACUACAAGAUACAG UGCCCCAGACGACUCGCCCGA 1447_ H32
TCCAGCCCGTTGTCGTTGGC GGGAGGACGAUGCGGUCCAGCCCGUUGUCGUUGGCCAGACGACUCGCCCGA 1448_H32
CG ATCGTAG GTCGTrG TG CC GGGAGGACGAUGCGGCGAUCGUAGGUCGUUGUGCCCAGACGACUCGCCCGA 1449_H32
ACGAAGCGTTTGTACGGGCC GGGAGGACGAUGCGGACGAAGCGUU 'JGUACGGGCCCAGACGACUCGCCCGA 1450„H32
GTCCTGTCGTGTGTTCGTCCC GGGAGGACGAUGCGGGUCCUGUCGUGUGUUCGUCCCCAGACGACUCGCCCGA 1451_H32
ATTGGTG6AGGGAGGGACCG GGGAGGACGAUGCGGAUUGGUGGAGGGAGGGACCGCAGACGACUCGCCCGA 1452_H32
GCCTTG TTCTG ACCTCCC GGGAGGACGAUGCGGGCCUUGUUCUGACCUCCCCAGACGACUCGCCCGA 1453_H32
GTTGTCATGACGTTCGTCCC GGGAG6AC6AUGCGGGUUGUCAUGACG UUCGUCCCCAGACGACUCGCCCGA 1454_H32
TCG ATCTTG G TGTCCCTTG C GGGAGGACGAUGCGGUCGAUCUUGGUG UCCCUUGCCAGACGACUCGCCCGA 1455 H32
# in H4
UTS read variable region RNA sequences P in H3 selection selection Selected sequenc
ATGATGTGTAGTAGCCGCCC GGGAGGACGAUGCGGAUGAUGUGUAGUAGCCGCCCCAGACGACUCGCCCGA 1456. H32
CATGAATGTTTCCCGCGCAC G GG AGG ACG AUG CG GCA UG AAUG U U U'CCCGCG CA CCAGACG AC U CG CCCGA 1457. H32
TCG CTAATCTCCTGTCTGTG GGGAGGACGAUGCGGUCGCUAAUCUCCUGUCUGUGCAGACGACUCGCCCGA 1458. H32
AGGTCCTCAGATTGCGTGCC GGGAGGACGAUGCGGAGGUCCUCAGAUUGCGUGCCCAGACGACUCGCCCGA 1459, H32
ATTGGAGAGGGGAGGGACTG GGGAGGACGAUGCGGAU UGGAGAGGGGAGGGACUGCAGACGACUCGCCCGA 1460_ .H32
TTGGG CGTAG6GTGG CAC CG GGGAGGACGAUGCGGUUGGGCGUAGGGUGGCACCGCAGACGACUCGCCCGA 1461_ .H32
TGTCGTCAACGCGTTCTGTG GGGAGGACGAUGCGGUGUCGUCAACGCGU UCUGUGCAGACGACUCGCCCGA 1462_ .H32
ATTGGTG AAG G G TG A ACCG GGGAGGACGAUGCGGAUUGGUGAAGGGUGAACCGCAGACGACUCGCCCGA 1463_ _H32
TTTGAAGGGCGATGGGTCCA GGGAGGACGAUGCGGUUUGAAGGGCGAUGGG UCCACAGACGACUCGCCCGA 1464_ .H32 4905 H04
ATTGCGCTACTCTCCTGTGC GGGAGGACGAUGCGGAUUGCGCUACUCUCCUGUGCCAGACGACUCGCCCGA 1465. H32
CCGTACTTTACAGTCGTGCC GGGAGGACGAUGCGGCCGUACUU UACAG UCGUGCCCA6ACGACUCGCCCGA 1466. .H32
TATTGGAGG TGTAG GA ACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAGGAACCGCAGACGAC'UCGCCCGA 1467. _H32
TCGTCACAAACTGTGTGCCC GGGAGGACGAUGCGGUCGUCACAAACUG UGUGCCCCAGACGACUCGCCCGA 146S_ H32
GAATTCACGTGTCGCTGGGC GGGAGGACGAUGCGGGAAUUCACGUGUCGCUGGGCCAGACGACUCGCCCGA 1469. .H32
TCGATTGTGTCGTTCTGCGG GGGAGGACGAUGCGGUCGAU UGUGUCGUUCUGCGGCAGACGACUCGCCCGA 1470_ _H32
AAGATTCTGGGACACTACTG GGGAGGACGAUGCGGAAGAUUCUGGGACACUACUGCAGACGACUCGCCC6A 1471. ^H32
CATCCTATCTTGTTCCG AC GGGAGGACGAUGCGGCAUCCUAUCUUGUUCCGCACCAGACGACUCGCCCGA 1472. H32
TCACTTGGTTTCGACTGCGC GGGAGGACGAUGCGGUCACUUGGUUUCGACUGCGCCAGACGACUCGCCCGA 1473. H32
ATGAGTGAGTTGTGT6CCCC GGGAGGACGAUGCGGAUGAG UGAGUUGUGUGCCCCCAGACGACUCGCCCGA 1474. _H32
GCTCCAATGTTGTCTGCGGC GGGAGGACGAUGCGGGCUCCAAU6UUGUCUGCGGCCAGACGACUCGCCCGA 1475. .H32
TGAGCCCATGGCTTTTTGGC GGGAGGACGAUGCGGUGAGCCCAUGGCUUUUUGGCCAGACGACUCGCCCGA 1476 . H32
GTGTCAGGATATATGCTCTG GGGAGGACGAUGCGGGUGUCAGGAUAUAUGCUCUGCAGACGACUCGCCCGA 1477. _H32
TTTG AAG GG GGATGG ATCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGAUCCGCAGACGACUCGCCCGA 1478. .H 32
TCCTGTCGTCTGTTCGTTC GGGAGGACGAUGCGG UCCUGUCGUCUGUUCGU UCCAGACGACUCGCCCGA 1479. H32 1228_H04
TTGATCCTCCTCTCGTGGAC GGGAGGACGAUGCGG UUGAUCCUCCUCUCGUGGACCAGACGACUCG CCCGA 1480. _H32
TTTGAAGGAAGATGGGTCCG GGGAGGACGAUGCGG UUUGAAGGAAGAUGGGUCCGCAGACGACUCGCCC A 1481. _H32 3373_H04
TTTGAA6GGTGGTTGGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGGU'UGGUCCGCAGACGACUCGCCCGA 1482. _H32 2"594_H04
TTTTAAGGGCGATGGGTCC6 GGGAGGACGAUGCGGUU UUAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 1483 H32 1589 H04
tt in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
A ATCGT AG CCTCC ATGTG CC GGGAGGACGAUGCGGAAUCGUAGCCUCCAUGUGCCCAGACGACUCGCCCGA 1484_H32
TTTGAAGGGAGATGGGTCTG GGGA.GGACGAUGCGGUUUGAAGGGAGAUGGGUCUGCAGACGACUCGCCCGA 1485_H32 3773 H04
ATCCGTAAGTCACCCGTGTG GGGAGGACGAUGCGGAUCCG UAAGUCACCCGUG UGCAGACGACUCGCCCGA 1486_H32
GTTTTG GTTGTGTGCCGCTG GGGAGGACGAUGCGGGUUUUGGUUGUGUGCCGCUGCAGACGACUCGCCCGA 1487_H32
TTTGAAGGGGGGTGGGACTG GGGAGGACGAUGCGGUUUGAAGGGGGGUGGGACUGCAGACGACUCGCCCGA 1488_H32
TTT6AAGGACGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGACGAUGGGUCCGCAGACGACUCGCCCGA 1489_H32 3607 H04
TGTAGGGAGGTGGTT ATCGT GGGAGGACGAUGCGGUGUAGGGAGGUGGU UAUCGUCAGACGACUCGCCCGA 1490„H32
TTGGGTGTAGGGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGUACCGCAGACGACUCGCCCGA 1491_H32
TTAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 1492_H32 4136 H04
TTTG6AGGGGGGTGGTACCG GGGAGGACGAUGCGGUUUGGAGGGGGGUG6UACCGCAGACGACUCGCCCGA 1493_ H32
TATTGGAGGTGTAGAGACCG GGGAGGACGAUGCGGUAU UGGAGGUGUAGAGACCGCAGACGACUCGCCCGA 1494_H 2
TGA6TGTCTCCCTTGGTCTG GGGAGGACGAUGCGGUGAGUG UCUCCCUUGGUCUGCAGACGACUCGCCCGA 1495_H32
GTTGAGTAGATCTGGTGCCG G6GAGGACGAUGCGGG UUGAG UAGAUCUGGUGCCGCAGACGACUCGCCCGA 1496_H32
o
GTATGAAGTCGCCGCGTCTG GGGAGGACGAUGCGGG UAUGAAGUCGCCGCGUCUGCAGACGACUCGCCCGA 1497_H32
GCCATAATCTTCTGCTGCCG GGGAGGACGAUGCGGGCCAUAAUCUUCUGCUGCCGCAGACGACUCGCCCGA 1498_ H32
GTCTTCTGGCCTATCGTCCC GGGAGGACGAUGCGGG UCUUCUGGCCUAUCGUCCCCAGACGACUCGCCCGA 1499_H32 4163 H04
ACTATGTCGTCTTG TCCCCA GGGAGGACGAUGCGGACUAUGUCGUCUUGUCCCCACAGACGACUCGCCCGA 1500_H32
TAGACTCCGTTCCCTGTCGC GGGAGGACGAUGCGGUAGACUCCGU UCCCUGUCGCCAGACGACUCGCCCGA 1501_H32
CG ATTCG ATCTGTTCGTCCC GGGAGGACGAUGCGGCGAUUCGAUCUGUUCG UCCCCAGACGACUCGCCCGA 1502_H32
AGTGAAGTTTGGAGTTGCGC GGGAGGACGAUGGGGAGLiGAAGU UUGGAGUUGCGCCAGACGACUCGCCCGA 1503_H32
GAAGTCTGTTTTTGCCGCGC GGGAGGACGAUGCGGGAAGUCUGU UUUUGCCGCGCCAGACGACUCGCCCGA 1504JH32
CATCTAGACTCCCG CGTGTG GGGAGGACGAUGCGGCAUCUAGACUCCCGCG UGUGCAGACGACUCGCCCGA 1505_H32
TTGTCTGCGATGGCGGTGGC GGGAG6ACGAUGCGGU UGUCU6CGAUGGCGGUGGCCAGACGACUCGCCCGA 1506JH32
GGCTTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGGCU UUCGUUCUGACCUCGCCAGACGACUCGCCCGA 15Q7_H32
TATTTGGTGAAGTGGAGGTG GGGAGGACGAUGCGGUAUU UGGUGAAGUGGAGG UGCAGACGACUCGCCCGA 1508_H32
CCCATAACCAATGTGTGCCC GGGAGGACGAUGCGGCCCAUAACCAAUGUGUGCCCCAGACGACUCGCCCGA 1509_H32
TGTGGTGTAACTGGTGACCC GGGAGGACGAUGCGGUGUGGUGUAACUGGUGACCCCAGACGACUCGCCCGA 1510_ H32
ATTG ACG TCG CTG CGCACTG GGGAGGACGAUGCGGAUUGACG UCGCUGCGCACU6CAGACGACUCGCCCGA 1511 H32
# in H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc
CACCCTAAGACCTCAATCCG GGGAGGACGAUGCGGCACCCUAAGACCUCAAUCCGCAGACGACUCGCCCGA 1512., _H32
CTAGTTCTCTCG TCG G TCTG GGGAGGACGAUGCGGCUAGUUCUCUCGUCGGUCUGCAGACGACUCGCCCGA 1513. .H32
ATTGGGGAAGGGAGGGACTG GGGAGGACGAUGCGGAUUGGGGAAGGGAGGGACUGCAGACGACUCGCCCGA 1514_ .H32
GTTCCTCGTCTCTGCATGTC GGGAGGACGAUGCGGGUUCCUCGUCUCUGCAUGUCCAGACGACUCGCCCGA 1515. .H32
TTTG AGGG GTG ATGG GTCCC GGGAGGACGAUGCGGU UUGAGGGGUGAUGGGUCCCCAGACGACUCGCCCGA 1516. _H32 1716 H04
AGTTCTGGACATGCCGCGAC GGGAGGACGAUGCGGAGUUCUGGACAUGCCGCGACCAGACGACUCGCCCGA 1517. .H32
TACTGGTTCCCACTTGTCTG GGGAGGACGAUGCGGUACUGGUUCCCACUUGUCUGCAGACGACUCGCCCGA 1518. .H32
TGTGTCTTGTCTGCTTGCTG GGGAGGACGAUGCGGUGUGUCUUGUCUGCUUGCUGCAGACGACUCGCCCGA 1519. .H32
CCGTTGTCTAG GTGTATG CC GGGAGGACGAUGCGGCCGUUGUCUAGGUGUAUGCCCAGACGACUCGCCCGA 1520. _H32
AAATCGACCTTCCACGTGGC GGGAGGACGAUGCGGAAAUCGACCUUCCACGUGGCCAGACGACUCGCCCGA 1521. _H32
TAAAGTCGTCAACACAGCGC GGGAGGACGAUGCGGUAAAGUCGUCAACACAGCGCCAGACGACUCGCCCGA 1522. H32
TTCATCCTTCGACGTCTGTG GGGA6GACGAUGCGGUUCAUCCUUCGACGUCUGUGCAGACGACUCGCCCGA 1523_ .H32
ATTAG GAG TGTAGTCG TGCC GGGAGGACGAUGCGGAUUAGGAGUGUAGUCG UGCCCAGACGACUCGCCCGA 1524. H32
o
CTG TCGTCTTG TTCGTCCC GGGAGGACGAUGCGGCUGUCGUCiJUGUUCGUCCCCAGACGACUCGCCCGA 1525. ^H32
TTTGAAG GGGGATGGGTCAG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGUCAGCAGACGACUCGCCCGA 1526. _H32
AACCAAGCCTCTCTGCGTGC GGGAGGACGAUGCGGAACCAAGCCUCUCUGCGUGCCAGACGACUCGCCCGA 1527. .H32
CTTGTCGTCTTGTTTGTCCC GGGAGGACGAUGCGGCUUGUCG UCUUGUUUGUCCCCAGACGACUCGCCCGA 1528. .H32
TTGGGTGAAGGGTGGTACTG GGGAGGACGAUGCGGU UGGGUGAAGGGUGG UACUGCAGACGACUCGCCCGA 1529. .H32
GAGTCATGTACACGATTGTG GGGAGGACGAUGCGGGAGUCAUGUACACGAUUGUGCAGACGACUCGCCCGA 1530. H32
TACGATAGGCTCATGCGCTG GGGAGGACGAUGCGGUACGAUAGGCUCAUGCGCUGCAGACGACUCGCCCGA 1531. H32
ACTG AG AATAATG GTG GCCG GGGAGGACGAU6CGGACUGAGAAUAAUGGUGGCCGCAGACGACUCGCCCGA 1532. H32
ACCTCCACACTATCGTGCCC GGGAGGAC6AUGCGGACCUCCACACUAUCGUGCCCCAGACGACUCGCCCGA 1533. .H32
CT ATTG GTGAAGGGGGGTG GGGAGGACGAUGCGGCUAUUGGUGAAGGGGGGUGCAGACGACUCGCCCGA 1534. .H32
TCCTCTTCTGTAGTCTGTGC GGGAGGACGAUGCGGUCCUCUUCUGUAGUCUG UGCCAGACGACUCGCCCGA 1535. _H32
TTTTTGGTGATGGGTCCG GGGAGGACGAUGCGGUUUU UGGUGAUGGGUCCGCAGACGACUCGCCCGA 1536. ,H32
TGAGACGTTCGGTACATCGC GGGAGGACGAUGCGGUGAGACG UUCGGUACAUCGCCAGACGACUCGCCCGA 1537. _H32
CATTGGAATCCCACTCTCCC GGGAGGACGAUGCGGCAUUGGAAUCCCACUCUCCCCAGACGACUCGCCCGA 1538. .H32
AATTCG TCAATGCG CTGCCC GGGAGGACGAUGCGGAAUUCGUCAAUGCGCUGCCCCAGACGACUCGCCXGA 1539 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AGTGAGCCTCTTCGATGCCG GGGAGGACGAUGCGGAGUGAGCCUCUUCGAUGCCGCAGACGACUCGCCCGA 1540. _H32
TTAGGGCATTCCTTCCTGCC GGGAGGACGAUGCGGU UAGGGCAUUCCUUCCUGCCCAGACGACUCGCCCGA 1541. „H32
GCAGGAGAGTTTAGCGGCGC GGGAGGACGAUGCGGGCAGGAGAGUUUAGCGGCGCCAGACGACUCGCCCGA 1542, .H32
TATCCTAGTCCCCTCGTCTG GGGAGGACGAUGCGGUAUCCUAG UCCCCUCGUCUGCAGACGACUCGCCCGA 1543. .H32
ACATGGCCTTGCACGTTGTC GGGAGGACGAUGC6GACAUGGCCUUGCACGUUGUCCAGACGACUCGCCCGA 1544_ _H32
GGAGAAACCCTGTGGTGTA GGGAGGACGAUGCGGGGAGAAACCCUGUGGUGUACAGACGACUCGCCCGA 1545. _H32
CTTGTCTCCGGTCTG CTGGC GGGAGGACGAUGCGGCU UG UCUCCGGUCU6CUGGCCAGACGACUCGCCCGA 1546. _H32
TAG TG TCTG GTATTG CACAC GGGAGGACGAUGCGGUAGUGUCUGG UAUUGCACACCAGACGACUCGCCCGA ^H32
ACAGTCGGATTGGGCCAGCA GGGAGGACGAUGCGGACAGUCGGAUUGGGCCACCACAGACGACUCGCCCGA 1548. _H32
ATTGG TG AAGG G AG GTTACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUUACUGCAGACGACUCGCCCGA 1549_ _H32
TTGGGTGTAGGGAGGGACCG GGGAGGACGAUGCGGUUGG6UGUAGGGAGGGACCGCAGACGACUCGCCCGA 1550. .H32
GTGTCTGAGTGTGCCTTCCC GGGAGGACGAUGCGGG UGUCUGAGUGUGCCU U 'CCCAGACGACUCGCCCGA 1551. H32
TTTGAAGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGAAGGUGGUGGGUCCGCAGACGACUCGCCCGA 1552_ H32
o
4- ATC6TTCTCTCTCCCGTGCC GGGAGGACGAUGCGGAUCGUUCUCUCUCCCGUGCCCAGACGACUCGCCCGA 1553. _H32
AGGAATACAGTCAAGGTCTG GGGAGGACGAUGCGGAGGAAUACAGUCAAGGUCUGCAGACGACUCGCCCGA 1554_ _H32
TCCAAACGAGCTCTGCTGCC GGGAGGACGAUGC6GUCCAAACGAGCUCUGCUGCCCAGACGACUCGCCCGA 1555. _H32
ATGAAGGCCCGTACACCCTG GGGAGGACGAUGCGGAUGAAGGCCCGUACACCCUGCAGACGACUCGCCCGA 1556. .H32
TGTCCTTTCTCCGTCTGCCG GGGAGGACGAUGCGGUGUCCUUUCUCCGUCUGCCGCAGACGACUCGCCCGA 1557. .H32
GTCCACCATGATGTTGTCTG GGGAGGACGAUGCGGG U'CCACCAUGAUGUUGUCUGCAGACGACUCGCCCGA 1558. .H32
TTCCTCGATCACCGATACTG GGGAGGACGAUGCGGU UCCUCGAUCACCGAUACIIGf AGACGACUCGCCCGA 1559. „H32
ACCATGTTTTAAGTGTGCGC GGGAGGACGAUGCGGACCAUGUU UUAAGUGUGCGCCAGACGACUCGCCCGA 1560. _H32
TAG T6TG AGTCG CCCTTGTG G6GAGGACGAUGCGGUAGUGUGAGUCGCCCUUGUGCAGACGACUCGCCC6A 1S61_ H32
TCCATTGCTTCTCGTCTGGC GGGAGGACGAUGCGGUCCAUUGCUUCUCGUCUGGCCAGACGACUCGCCCGA 1562. .H 2
TG ATACAAATTTTACTG CTG GGGAGGACGAUGCGGUGAUACAAAUUUUACUGCUGCAGACGACUCGCCCGA 1563. .H32
TTTG TAC AACG GTCTG G C G C GG6AGGACGAUGCGGUUUGUACAACGGUCUGGCGCCAGACGACUCGCCCGA 1564. .H32
TACC ACATATAGACTG GG CC GGGAGGACGAUGCGGUACCACAUAUAGACUGGGCCCAGACGACUC6CCCGA 1565, ,H32
ATTGCCCATATCCTCCT6CC GGGAGGACGAUGCGGAUUGCCCAUAUCCUCCUGCCCAGACGACUCGCCCGA 1566. .H32
ATCGTTTGTGGTATGGTGGC GGGAGGACGAUGCGGAUCGU UUGUGCUAUGG UGGCCAGACGACUCGCCCGA 1567 H32
# ίη Η4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
GCCTTTTCGTCTTGCCTGCC GGGAGGACGAUGCGGGCCUUUUCGUCUUGCeUCCCCAGACGACUCGCCCGA 156S_H32
ATTGGCGAAGGGAGGCACTG GGGAGGACGAUGCGGAUUGGCGAAGGGAGGCACUGCAGACGACUCGCCCGA 1569_H32
TGTA6GAGGAGGAGTACCG GGGAGGACGAUGCGGUGUAGGAGGAGGAGUACCGCAGACGACUCGCCCGA 1570_H32
TTCCTCTATCCTCGTGTCCC GGGAGGACGAUGCGGUUeCUCUAUCCUCGUGUCCCCAGACGACUCGCCCGA 1571_H32
ATAGGGAGAGGGGTGAACCG GGGAGGACGAUGCGGAUAGGGAGAGGGGUGAACCGCAGACGACUCGCCCGA 1572JH32
CGAATAGGTCTCGCCATGGC GGGAGGACGAUGCGGCGAAUAGGUCUCGCCAUGGCCAGACGACUCGCCCGA 1573_H32
TGACTCGTCTGTTTCGTTCC GGGAGGACGAUGCGGUGACUCGUCUGUUUCGUUCCCAGACGACUCGCCCGA 1574_H32
TCCACTGTTCCC CCTCCC GGGAGGACGAUGCGGUCCACUGUUCCCAACCUCCCCAGACGACUCGCCCGA 1575„H32
TTTGGTGGGGGGTGGGACCG GGGAGGACGAUGCGGUUUGGUGGGGGGUGGGACCGCAGACGACUCGCCCGA 1576_H32
GCCTTTCGTTCT GCCCACC GGGAGGACGAUGCGGGCCU U UCGUUCUGCGCACCCAGACGACUCGCCCGA 1577_H32 2444_ H04
ACC6ACGAACAATGTGGCCC GGGA6GACGAUGCGGACCGACGAACAAUGUGGCCCCAGACGACUCGCCCGA 157S_H32
CACCGTTAGCGTTTGCTGGC GGGA6GACGAUGCGGCACCGUUAGCGU UUGCUGGCCAGACGACUCGCCCGA 1579_H32
TTGGAAGTAGGGTGGGACCG GGGAGGACGAUGCGGUUGGAAGUAGGGUGGGACCGCAGACGACUCGCCCGA 15S0_H32
ο
TGTCCATATCGTAACCTCCC GGGAGGACGAUGCGGUGUCCAUAUCG UAACCUCCCCAGACGACUCGCCCGA 1581_H32
TTTG AAGG G GGGTG GTACCG GGGAGGACGAUGCGGU UUGAA.GGGGGGUGGUACCGCAGACGACUCGCCCGA 1582_ H32
ICG GACT AGTCG TTTTG CG C GGGA6GACGAUGCGGUCGGACUAGUCGUUUUGCGCCAGACGACUCGCCCGA 1583_H32
TCCTTTGGTTCTGAGCTCCC GGGAGGACGAUGCGGUCCUU UCGUUCUGAGCUCCCCAGACGACUCGCCCGA 1584_H32
TTTGACGG GTGCTG GG TCCG GGGA6GACGAUGCGGUUUGACGGGUGCUGGGUCCGCAGACGACUCGCCCGA 15S5_H32 4373_ H04
AT7GGTGACGG6AGGTACCG GGGAGGACGAUGCGGAUUGGUGACGGGAGGUACCGCAGACGACUCGCCCGA 15S6_H32
TTTGAAGGGAGATGGGTCCA GGGAGGACGAUGCGGUUUGAAGGGAGAUGGGUCCACAGACGACUCGCCCGA 1587_H32 4162_ H04
TTTG AAGGGAG ATAG GTCCG GGGA6GAC6AUGCGGUUUGAAGGGAGAUAGGUCCGCAGACGACUCGCCCGA I5S8_ H32 3919_ H04
GCCTTTCGTTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUUCUGCCCUCCCCAGACGACUCGCCCGA 1589_H32
GTTGATGTCTTGGGCTGCTG GGGAGGACGAUGCGGG UUGAUGUCUUGGGCUGCUGCAGACGACUCGCCCGA 1590_ H32
GCCTTTCGTTCTGCCTTCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCUUCCCCCAGACGACUCGCCCGA 1591_H32 1869_ _H04
TTG6GT6TAGGGCGGGACCG GGGAGGACGAUGCGGU UGGGUGUAGGGCGGGACCGCAGACGACUCGCCCGA 1592_H32
GTTGAAGGGTGATGGGCCCG GGGAGGACGAUGCGGGUUGAAGGGUGAUGGGGCC6CAGACGACUCGCCCGA 1593_.H32
TTTGAAGGGGGATTGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUUGGUCCGCAGACGACUCGCCCGA 1594J-B2 4182_ .HQ4
ATTGGTGAAGGGAGGTCTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUQJGCAGACGACUCGCCCGA 1595 H32
# in H4
HTS read variable region RNA sequences » in H3 selection selection Selected sequenc
TTTGCAGGGTGATGGGCCCG GGGAGGACGAUGCGGUUUGCAGGGUGAUGGGCCCGCAGACGACUCGCCCGA 1596_ .H32 40 3_H04
ATTGGAGAAGGGTGGGACCG GGGAGGACGAUGCGGAUUGGAGAAGGGUGGGACCGCAGACGACUCGCCC6A 1597. _H32
ΪΤΤ G AAG GG AG ATTG GTCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUUGGUCCGCAGACGACUCGCCCGA 1598. .H32 4483 H04
CTTGTCGTCTTGTTCGTTCC GGGAGGACGAUGCGGCUUG UCGUCUUGUUCGUUCCCAGACGACUCGCCCGA 1599^ _H32
TG GGTC GTCTTCTGTTCCCC GGGAGGACGAUGCGGUGGGUCGUCUUCUGU UCCCCCAGACGACUCGCCCGA 160Q_ _H32
TTGGCATATCACACGGTGTC GGGAGGACGAUGCGGUUGGCAUAUCACACGGUGUCCAGACGACUCGCCCGA 1601. H32
TIG GTCGTCTG AA ACCTCCC GGGAGGACGAUGCGGUUGGUCGUCUGAAACCUCCCCAGACGACUCGCCCGA 1602. .H32
TGGATCGTTTGGTGACACGC GGGAG6ACGAUGCGGUGGAUCGUUUGGUGACACGCCAGACGACUCGCCCGA 1603. _H32
A6TCCCAA CTGTTG TTGCTG GGGAGGACGAUGCGGAGUCCCAACUGU UGUUGCUGCAGACGACUCGCCCGA 1604. .H32
AAAA.GGGTCTTCGTCTGCGC GGGAGGACGAUGCGGAAAAGGG UCU UCGUCUGCGCCAGACGACUCGCCCGA 1605. _H32
CCGAGTCTTCCTGCGTGCGC GGGAGGACGAUGCGGCCGAGUCUUCCUGCG UGCGCCAGACGACUCGCCCGA 1606. .H32
AAACCGTAGCCGCACGTGGG GGGAGGACGAUGCGGAAACCGUAGCCGCACGUGGGCAGACGACUCGCCCGA 1607. ^H32
TGGAATGTTACCTCTAGCTG GGGAGGACGAUGCGGUGGAAUGUUACCUCUAGCUGCA6ACGACUCGCCCGA
o 1608. .H32
CCTGTCGCACTGC6GTACTG GGGAGGACGAUGCGGCCUGUCGCACUGCGGUACUGCAGACGACUCGCCCGA 1609_ .H32
TGTCCCCGAGTCTGTGTGGC G6GAGGACGAUGCGGUGUCCCCGAGUCUGUG UGGCCAGACGACUCGCCCGA 1610. .H32
TGCGATGCGTCCGATCTCGC GGGAGGACGAUGCGGUGCGAUGCGUCCGAUCUCGCCAGACGACUCGCCCGA 1611- ,H32
GTTCTTGGCGTCTTATACTG GGGAGGACGAUGCGGG UUCUUGGCGUCUUAUACUGCAGACGACUCGCCCGA 1612. H32
CCCGCTGTATTCCTCGTCCC 6GGAGGACGAUGCGGCCCGCUGUAUUCCUCGUCCCCAGACGACUCGCCCGA 1613. ^H3
CTTGTCGTCTTGTTCGTCC GGGAGGACGAUGCGGCUUGUCGUCUU6UUCG UCCCAGACGACUCGCCCGA 1614. _H32
GAATCCGTGTGATATG GCTG GGGAGGACGAUGCGGGAAUCCG UGUGAUAUGGCUGCAGACGACUCGCCCGA 1615_ _H32
TCCGTAAATGTTGTTGTCGC GGGAGGACGAUGCGGUCCGUAAAUGUUGUUGUCGCCAGACGACUCGCCCGA 1616. .H32
ATAGTCTACACATGACTGCC GGGAGGACGAUGCGGAUAG UCUACACAUGACUGCCCAGACGACUCGCCCGA 1617, ,H32
ACCTACGTCTTTTCCGTGTG GGGAGGACGAUGC6GACCUACGUCUU UUCCGUGUGCAGACGACUCGCCCGA 1618. _H32
TCCCATGCCTTCCTGTACCG GGGAGGACGAUGCGGUCCCAUGCCUUCCUGUACCGCAGACGACUCGCCCGA 1619, H32
TCACCAAACTCGTGCGCCC GGGAGGACGAUGCGGUCACCAAACUCGUGCGCCCCAGACGACUCGCCCGA 1620. _H32
CATCTACCTTCCTACGTGTC GGGAGGACGAUGCGGCAUCUACC UUCCUACGUG UCCAGACGACUCGCCCGA 1621. _H32
TGTTGTTGTGT6CGGTTGTG GGGAGGACGAUGCGGUGUUGUUG UGUGCGGUUGUGCAGACGACUCGCCCGA .1622. H32
GCGTCTGAATTCTCGCTCCC GGGAGGACGAUGCGGGCGUCUGAAUUCUCGCUCCCCAGACGACUCGCCCGA 1623 H32
8 in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TATCGTAACTCATGCGTGCC GGGAGGACGAUGCGGUAUCGUAACUCAUGCGUGCCCAGACGACUCGCCCGA 1624_ _H32
AG TGTCTCTGGGTTCACGC GGGAGGACGAUGCGGAGUGUCUCUGGGUUUCACGCCAGACGACUCGCCCGA 1625. .H32
AATTGTGCTCCTCCCATG6C GGGAGGACGAUGCGGAAUUGUGCUCCUCCCAUGGCCAGACGACUCGCCCGA 1626. ,H32
TTCCCGATCCTTCXCATGTG GGGAGGACGAUGCGGUUCCCGAUCCUUCCCAUGUGCA6AC6ACUCGCCCGA 1627. ..H32
GTTGAGTGTGTCATGCCCCC GGGAGGACGAUGCGGGUUGAGUG UGUCAUGCCCCCCAGACGACUCGCCCGA 1628. _H32
GACCTTAGTT6GACACTGTG GGGA6GACGAUGCG6GACCUUAG UUGGACACUGUGCAGACGACUCGCCCGA 1629. _H32
GTACG GTT6GTTTG CTGCGC GGGAGGACGAUGCGGG UACGGU UGGUUUGCUGCGCCAGACGACUCGCCCGA 1630. _H32
TCACTG CCACTAGCA ATCCG GGGAGGACGAUGCGGUCACUGCCACUAGCAAUCCGCAGACGACUCGCCCGA 1631. H32
CAGTCGTCATGTACCGCGTC GGGAGGACGAUGCGGCAGUCGUCAUGUACCGCGUCCAGACGACUCGCCCGA 1632_ .H32
TCCACGAATAGTG CACTGCC GGGAGGACGAUGCGGUCCACGAAUAGUGCACUGCCCAGACGACUCGCCCGA 1633. .H32
TTTGCGATTTCTCCCTGGC GGGAGGACGAUGCGGUUUGCGAUUUCUCCCUGGCCAGACGACUCGCCCGA 1634. H32
G AACATGG CGTG CCTCTGTG GGGAGGACGAUGCGGGAACAUGGCGUGCCUCUG UGCAGACGACUCGCCCGA 1635. _H32
CCCG CAATAGTCCCAGTG CC GGGAGGACGAUGCGGCCCGCAAUAGUCCCAGUGCCCAGACGACUCGCCCGA 1636_ H32
o
ATTGGAGGTGATGGGTCCG GGGA6GACGAUGCGGAUUGGAGGUGAUGGGUCCGCAGACGACUCGCCCGA 1637. H32
CTTGTCGGCTTGTTCGTCCC GGGA6GACGAUGCGGCU UGUCGGCUUGUUCGUCCCCAGACGACUCGCGCGA 1638. .H32
C ATCG CGTATCCCACG TCTG GGGAGGACGAUGCGGCAUCGCGUAUCCCACGUCUGCAGACGACUCGCCCGA 1639. .H32
6CCATGTTAGTCATGGGGGG GGGAGGACGAUGCGGGCCAUGUUAGUCAUGGGGGGCA6ACGACUCGGCCGA 1640. .H32
TTTGAAGGGTGATGGGGTCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGGUCGCAGACGACUCGCCCGA 1641. H32
CATGTGGGTTAGGCCGTCTG GGGAGGACGAUGCGGCAUGUGGGUUAGGCCGUC JGCAGACGACUCGCCCGA 1642. _H32
TCGTGTAAGTGTGTTGTGTG GGGAGGACGAUGCGG UCGUG UAAGUGUGliUGIJG UGCAGACGACUCGCCCGA 1643. H32
TACCAAGCACGTTCGTTGGC GGGAGGACGAUGCGGUACCAAGCACGUUCGUUGGCCAGACGACUCGCCCGA 1δ44. JH32
TCAGATGTACGATTGGTGTG GGGAGGACGAU6CGGUCAGAUGUACCAUUGGUGUGCAGACGACUCGCCCGA 1645. „H32
TGATGTG ATG GTTG GTGCTG GGGAGGACGAUGCGGUGAUGUGAUGGUUGGUGCUGCAGACGACUCGCCCGA 1646. .H32
GGTTTGAGCGTCCTTGTGTG GGGAGGACGAUGCGGGGUU UGAGCGUCCUUGUGUGCAGACGACUCGCCCGA 1647. .H32
TGTTTG CTAATAACGCTGTG GGGAGGACGAUGCGGUGUUUGCUAAUAACGCUGUGCAGACGACUCGCCCGA 1648. _H32
AGGCTTGTCTGCCGTTGGTG GGGAGGACGAUGCGGAG6CU UGUCUGCCGU UGGUGCAGACGACUCGCCCGA 1649. _H32
AATTTGGTGGAAACGCGCTG GGGAGGAC6AUGCGGAAUUUGGUGGAAACGCGCUGCAGACGACUCGCCCGA 1650. JH 32
TTTGGAGGGTGATGGGTCCC GGGAGGACGAUGCGGUUUGGAGGGUGAU6GGUCCCCA6ACGACUCGCCCGA 1651 H32 3265 H04
# in H4
HTS read variable region RNA sequences # In H3 selection selection Selected sequenc TGTAGTCTTAACCCGATGGC GGGAGGACGAUGCGGUGUAGUCUUAACCCGAUGGCCAGACGACUCGCCCGA 1652. _H32
TGAGTTGTCCCAATCTGCTG GGGAGGACGAUGCGGUGAG UUGUCCCAAUCUGCUGCAGACGACUCGCCCGA 1553. _H32
TGCGTTACATGATTGTGCGC GGGAGGACGAUGCGGUGCGUUACAUGAUUGUGCGCCAGACGACUCGCCCGA 1654_ H32
TGACTCGTCTGCTTCGTCCC GGGAGGACGAUGCGGUGACUCGUCUGCUUCGUCCCCAGACGACUCGCCCGA 1655. _H32
TCCTTGTACGTTCTACCCCC GGGAGGACGAUGCGGUCCUUGUACGUUCUACCCCCCAGACGACUCGCCCGA 1656. _H32
TT6GGTGTAGGGTGGTACCCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGG UACCCGCAGACGACUCGCCCGA 1657. _H32
ATACTGTTTGCGTGCTGCCC GGGAGGACGAUGCGGAUACUGU UUGCGUGCUGCCCCAGACGACUCGCCCGA 658, .H32
GTCA'ITGTTGTCTGTTGCCC GGGAGGACGAUGCGGGUCAU UGUUGUCUGUUGCCCCAGACGACUCGCCCGA 1659. H32
ACCGTATCGTATTGTGTGTG GGGAG6ACGAUGCGGACCGUAUCGUAUUGUGUGUGCAGACGACUCGCCCGA 1660. _H32
TACTGTCGTCTGTTCGTC CC GGGAGGACGAUGCGGUACUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1661. _H32 0386 H04
TTTCCTCGTGTTCGTGTGTG GGGAGGACGAUGCGGUUUCCUCG 'JGUUCGUGUGUGCAGACGACUCGCCCGA 1662. .H32
CGACACGCTTAATATGGCTG GGGAGGACGAUGCGGCGACACGCU UAAUAUGGCUGCAGACGACUCGCCCGA 1663. H32
CACACGCTATTCCCCGTCTG GGGAGGACGAUGC6GCACACGCUAUUCCCCGUCUGCAGACGACUCGCCCGA 1664. _H32
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TTTGGTGTAGGGA6GTACTG GGGAGGACGAUGCGGUUUGGUGUAGG6AGGUACUGCAGACGACUCGCCCGA 1665. _H32
ACAG TG TGTTCTGCCTTGTG GGGAGGACGAUGCGGACAGUGUGUUCUGCCUUGUGCAGACGACUCGCCCGA 1666, .H32
AACCTCTCTGTCG TTGAGTG GGGAGGACGAUGCGGAACCUCUCUGUCGUUGAGUGCAGACGACUCGCCCGA 1667. .H32
GTCTTTCGACGTCTTGGTAC GGGAGGACGAUGCG6GUCUUUCGACGUCUUGGUACCAGACGACUCGCCCGA 1668. ,H32
TCACTGATGGGTTGTACTG GGGAGGACGAUGCGGUCACUGAUGGGUUGUACUGCAGACGACUCGCCCGA 1669, _H32
TCCATCAACAACTTGCTCCG GGGAGGACGAUGCGGUCCAUCAACAACUUGCUCCGCAGACGACUCGCCCGA 1670. _H32
TTGCATTGCTCCTGCGT6TG GGGAGGACGAUGCGGUUGCAUUGCUCCUGCGUGUGCAGACGACUCGCCCGA 1671. .H32
TGTAGG G GGGTAGTTATCGT GGGAGGACGAUGCGGUGUAGGGGGGUAGUUAUCGUCAGACGACUCGCCCGA 1672. .H32
TTCGAGGGGTGATGGGTCCG GGGAGGACGAUGCGGUUCGAGGGGUGAUGGG UCCGCAGACGACUCGCCCGA 1673. H32
TTAAGTGTAG G GTGGTACCG 6G6AGGACGAUGCGGUUAAGUG UAGGGUGGUACCGCAGACGACUCGCCCGA 1674. _H32
ATTGGTGAAGGGAGGTACG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUACGCAGACGACUCGCCCGA 1675. .H32
TTTGAAGGGGGATGGTACCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGUACCGCAGACGACUCGCCCGA 1676. .H32
TT6GGAGTAGGGTGGAACCG GGGAGGACGAUGCGGUUGGGAGUAGGGUGGAACXGCAGACGACUCGCCCGA 1677. .H32
TTTG AAG G G C G AT G AG TCCG GGGAG6ACGAUGCGGUU UGAAGGGCGAUGAGUCCGCAGACGACUCGCCCGA 1678. H32 3768 H04
ATCAGTGTAAA6TTTGTGTG GGGAGGAC6AUGCGGAUCAG UGUAAAGUUUGUGUGCAGACGACUCGCCCGA 1679 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TCCTGTCGTCTGTCTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUCUG UCCCCAGACGACUCGCCCGA 16SO_H32 0450. _H04
TTTGGTGAAGG6TGGTACCG GGGAGGACGAUGCGGU UUGGUGAAGGG UGGUACCGCAGACGACUCGCCCGA 1681_H32
ATAGTTGTCTTGTGTTTGTG GGGAGGACGAUGCGGAUAGUUGUCUUGUGU UUGUGCAGACGACUCGCCCGA 1682_H32
ATT6AAGGGAGATGGGTCCG GGGAGGACGAUGCGGAUUGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1683„H32 4332_ _H04
TGGTTGGTACGAGGGCTGTG GGGAGGACGAUGCGGUGGUUGGUACGAGGGCUGUGCAGACGACUCGCCCGA 1684_H32
TATTGGCG6TGTAGGGACCG GGGAGGACGAUGCGGUAUUGGCGGUGUAGGGACCGCAGACGACUCGCCCGA 1685_H32
GCTTGTCTTCTGTTCGTCCC GGGAGGACGAUGCGGGCUUGUCUUCUGUUCGUCCCCAGACGACUCGCCCGA 1686_H32
CGTGACTTTCCTCCGGTCGC GGGAGGACGAUGCGGGGUGACUUUCCUCCGGUCGCCAGACGACUCGCCCGA 1687_H32
GTTTCCGATGGTGGTGTGGC GGGAGGACGAUGCGGG UUUCCGAUGGUGGUGuGGCCAGACGACUCGCCCGA 1688_H32
TTTGAT6GGTGATGGGACCG GGGAGGACGAUGCGGU UUGAUGGGUGAUGGGACCGCAGACGACUCGCCCGA 1689_H32 5258_ _H04
TCAACGTTCGTCTCCTGGC GGGAGGACGAUGCGGUCAACGUUCGUCUCCUGGCCAGACGACUCGCCCGA 1690._Η32
TATTCTCTTCCCTGTCGCAC GGGAGGACGAUGCGGUAUUCUCUUCCCUGUCGCACCAGACGACUCGCCCGA 1691_H32
TTTGGTCCTCGCCGAGTCAC GGGAGGACGAUGCGGUUUGGUCCUCGCCGAGUCACCAGACGACUCGCCCGA 1692„H32
o
CCACCGTGAAAATGTGTGCC GGGAGGACGAUGCGGCCACCG UGAAAAUGUGUGCCCAGACGACUCGCCCGA 1693_H32
TGTTCCCTGCAAATCGTGTG GGGAGGACGAUGCGGU6UUCCCUGCAAAUCGUGUGCAGACGACUCGCCCGA 1S94_H32
TTTGAAGGGCGATGCGTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGCGUCCGCAGACGACUCGCCCGA 1695„H32 2145. _H04
TTTGGAGGGTAATGGGTCC6 GGGAGGACGAUGCGGUUUGGAGGGUAAUGGGUCCGCAGACGACUC6CCCGA 1695_H32
TTCGATTATGCCTGCCACCC GGGAGGACGAUGCGGU UCGAUUAUGCCUGCCACCCCAGACGACUCGCCCGA 1697_H32
TTTCGCATTGGTTGTGGCTG GGGAGGACGAUGCGGU UUCGCAUUGGUUGUGGCUGCASACGACUCGCCCGA 1698_K32
GTCCTGTCGTCTGTTCGTTCC GGGAGGACGAUGCGGGUCCUGUCGUCUGUUCG UUCCCAGACGACUCGCCCGA 1699_H32 3162.. . H04
TGTTG G CACTATAG CTG CTG GGGAGGACGAUGCGG UG UUGGCACUAUAGCUGCUGCAGACGACUCGCCCGA 1700_H32
TCCACCAGTTCG ACTCGTG C GGGAGGACGAUGCGGUCCACCAGU UCGACUCG UGCCAGACGACUCGCCCGA 1701_H32
6TACACATTG ATG ATGG CG C GGGAGGACGAUGCGGGUACACAUUGAUGAUGGCGCCAGACGACUCGCCCGA 1702J-B2
CGTCAGATCGTTCACGTGTC GGGAGGACGAUGCGGCGUCAGAUCGUUCACGUG UCCAGACGACUCGCCCGA 17Q3_ H32
TTTG GTG AAG GGTGG G ACTG GGGAGGACGAUGCGGUUUGGUGAAGGGUGGGACUGCAGACGACUCGCCCGA 1704_H32
ATTGGAGAGGGGAGGTACTG GGGAGGACGAUGCGGAU UGGAGAGGGGAGGUACUGCAGACGACUCGCCCGA 1705_H32
ATTGGTGAAGGGGGGTGCTG GGGAGGACGAUGCGGAUUGGUGAAGGGGGGUGCUGCAGACGACUCGCCCGA i706_H32
TTGGGTGCAGGGTGTTACCG GGGAGGACGAUGCGGUUGGGUGCAGGGUG UUACCGCAGACGACUCGCCCGA 1707 H32
# ln H4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc
TTTG CTG GG TG ATG G G TCCG GGGAGGACGAUGCGGUUUGCUGGG UGAUGGGUCCGCAGACGACUCGCCCGA 1708. H32
TTGGGGGTAGGGTGGGACCG GGGAGGACGAUGCGGUUGGGGGUAGGGUGGGAC.C.GCAGACGACUCGCCCGA 1709, H32
TTTGAGGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1710_ _H32
ATTGGTGAAG G GTT A CTG GGGAGGACGAUGCGGAUUG6UGAAGGG UUACUGCAGACGACUCGCCCGA 1711. .H32
TTTGAAGGGTGGTGGGGCCG GGGAGGACGAUGCGGUUUGAAGGGUGGUGGGGCCGCAGACGACUCGCCCGA 17-12. .H32 3867. ,HQ4
TTTTAAGGGAGATGGGTCCG GGGAGGACGAUGCGGUUUUAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1713.. _H32 3713. H04
TTTG AAG G G CAATG G GTCCG GGGAGGACGAUGCGGUUUGAAGGGCAAUGGGUCCGCAGACGACUCGCCCGA 3-71 , .H32 3203. H04
GTTGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGGUUGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 1715. _H32 2396. H04
ATTGGTGAGGGGTGGGACTG GGGAGGACGAUGCGGAUUGGUGAGGGGUGGGACUGCAGACGACUCGCCCGA 1716. H32
ATTGGTG AA6G GCG GCACTG GGGAGGACGAUGCGGAUUGGUGAAGGGCGGCACUGCAGACGACUCGCCCGA 1717. .H32
TTTG A AAG GAG ATG G GTCCG GGGAGGACGAUGCGGUUUGAAAGGAGAUGGGUCCGCAGACGACUCGCCCGA 1718_ H32
TT6GGAGTGGGATGGGACCG GGGAGGACGAUGCGGUUGGGAGUGGGAUGG6ACCGCAGACGACUCGCCCGA 1719. .H32
GCCCTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCCU UUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 1720. .H32 2996. HG4
TATTGGAGGTATAGGGACCG GGGAGGACGAUGCGGUAUUGGAGGUAUAGGGACCGCAGACGACUCGCCCGA 1721_ 32
GTCCTGTCGTCTTTTCGTCCC GGGAGGACGAUGCGGG UCCUGUCG UCUUUUC6UCCCCAGACGACUCGCCCGA 1722_ _H32 1864. H04
ATTGGAGGAGGGTGGGACCG GGGAGGACGAUGCGGAUUGGAGGAGGGUGGGACCGCAGACGACUCGCCCGA 1723_ H32
TTTGAAGGGTGATGGGTATG GGGA.GGACGAUGCGGU UUGAAGGGUGAUGGGUAUGCAGACGACUCGCCCGA 1724. _H32
TTTGAAGGGTGATGGGGCCC GGGAGGACGAUGCGGU UUGAAGGGUGAUG6GGCCCCAGACGACUCGCCCGA 1725. H32 4123. HG4
TTTG CCGGGTGATGG GTCCG GGGAGGACGAUGC6GUUUGCCGGGUGAUGGGUCCGCAGACGACUCGCCC6A 1726. H32 3027. .H04
TTTAAA6 G G AG ATGG GTCCG GGGAGGACGAUGCGGU UUAAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 1727. H32
TTGGGTGTGGGGTGTTACCG GGGAGGACGAUGCGGU UGGGUGUGGGGUG UUACCGCAGACGACUCGCCCGA 1728. _H32
TTTG AAG G CCG ATGGGTCCG GGGAGGACGAUGCGGU UUGAAQGCCGAUGGGUCCGCAGACGACUCGCCCGA 1729. _H32 4941, H04
ATTGGGGAAGGGAGGCACTG GGGAGGACGAUGCGGAUUGGGGAAGGGAGGCACUGCAGACGACUCGCCCGA 1730. .H32
ATTGGTG AAGG GAG GTAC GGGAGGACGAUGCGGAUUGGUG.AAGGGAGGUACCAGACGACUCGCCCGA 1731. .H32
ATTGGCGAAGGGAGGAACTG GGGAGGACGAUGCGGAUUGGCGAAGGGAGGAACUGCAGACGACUCGCCCGA 1732. H32
TTCTCCAAGTGTCGTGTGTG GGGAGGACGAUGCGGUUCUCCAAGUGUCGUGUGUGCAGACGACUCGCCCGA 1733. H32
TTGAGTACTTGTCTGTTCCC GGGAGGACGAUGCGGUUGAGUACUUGUCUG UUCCCCAGACGACUCGCCCGA 1734_ H32
AGTGGGTCGTATTCCGTGTG GGGAGGACGAUGCGGAGUGGGUCG UAUUCCGUG UGCAGACGACUCGCCCGA 1735 H32
# in H4
HTS read variable region RNA sequences » in H3 selection selection Selected sequenc
CAG CG TCTC ATCTTC GTGTG GGGAGGACGAUGCGGCAGCG UCUCAUCUUCGUGUGCAGACGACUCGCCCGA 1736_ _H32
TC6TTTGTTTCTGCTCCCCA GGGAG6ACGAUGCGGUCGUUUGU UUCUGCUCCCCACAGACGACUCGCCC6A 1737_ H32
AGTCCCCAACTAGTTGTCT6 GGGAGGACGAUGCGGAGUCCCCAACUAGUUGUCUGCAGACGACUCGCCCGA 1738. H32
TCCGCGTCTCCTTCGTGCC GGGAGGACGAUGCGGUCCGCGUCUCCUUCGUGCCCAGACGACUCGCCCGA 1739_ H32
CGTCGTTCCTTTCCCCCC GGGAGGACGAUGCGGUUCGUCGUUCCUUUCCCCCCCAGACGACUCGCCCGA X74Q_ H32
TGAGTATTTCTGTTCCCCCC GGGAGGACGAUGCGGUGAGUAUUUCUGUUCCCGGCCAGACGACUCGCCCGA 1741_ H32
CAGTCGTTTTACCGCGTGTG GGGAGGACGAUGCGGCAGUCGUUUUACCGCGUGUGCAGACGACUCGCCCGA 1742_ H32
TTTG GAG GAG G ATG GTACCG 6GGAG6ACGAUGCGGUUUGGAGGAGGAUGGUACCGCAGACGACUCGCCCGA 1743_ H32
GTGAAGTTCTCTTGCGT7GC GGGAGGACGAUGCGGGUGAAG UUCUCUUGCGUUGCCAGACGACUCGCCCGA 1744_ H3
CATCTGTCCAGTCC G GT AG C GGGAGGACGAUGCGGCAUCUG'JCCAGUCCGGUAGCCAGACGACUCGCCCGA 1745_ H32
G TCG CCTTG A ATG ACTG CG C GGGAGGACGAUGCGGGUCGCCUUGAAUGACUGCGCCAGACGACUCGCCCGA 1746_ .H32
T7TATG CCTCTC ACG TGCTG G GG AGG ACG A UGCG G U U U AUGCCU C U CAC6 UG CU G CAG ACGACUCG CCCG A 1747_ .H32
AC GTC G A ACTCCCG TG TC G C GGGAGGACGAUGCGGACGUCGAACUCCCGUGUCGCCAGACGACUCGCCCGA 1748_ H32
TTGTAGTCGTTTTCATTCGC GGGAGGACGAUGCGGUUGUAG UCGUUUUCAUUCGCCAGACGACUCGCCCGA 1749_ H32
TGCCTCTGTCCGTTCGTGTG GGGA6GACGAUGCGGUGCCUCUGUCCGU UCGUGU6CAGACGACUCGCCCGA 1750_ H32
ATG GCTGTTC GTTC6CTCCC GGGAGGACGAUGCGGAUGGCUGUUCGUUCGCUCCCCAGACGAC UCGCCCGA 1751_ H32
GTGAGGAAATCATTGTGCAC GGGAGGACGAUGCGGGUGAGGAAAUCAUUGUGCACCAGACGACUCGCCCGA 1752_ H32
TGGACGTGTTCCCGTCTGTG GGGAGGACGAUGCGGUGGACGUGUUCCCGUCUGUGCAGACGACUCGCCCGA 1753_ .H32
TACTATCCTCCCTTGGTCTG GGGAGGACGAUGCGGUACUAUCCUCCCUUGGUCUGCAGACGACUCGCCCGA 1754_ H32
TCCTTCGTCGTTTGGCTCAC GGGAGGACGAUGCGGUCCUUCGUCGUUUGGCUCACCAGAGGACUCGCCCGA 1755_ H32
CTGGGTGTAGGGAGGTACCG GGGAGGACGAUGCGGCUGGGUGUAGGGAGGUACCGCAGACGACUCGCCCGA 1756_ H32
TAGAGTGTCTCACTCTGTG GGGAGGACGAUGCGGUAGAGUGUCUCACUCCUGUGCAGACGACUCGCCCGA "1757_ ,H32
TTACTCGTCCACATTGTGCC GGGAGGACGAUGCGGU UACUCGUCCACAUUGUGCCCAGACGACUCGCCCGA 1758. H32
CAACACGTTCTCTTGTCGTG GGGAGGACGAUGCGGCAACACGUUCUCUUGUCGUGCAGACGACUCGCCCGA 1759_ H32
TTAGTCGAGGCCACTTGTGC GGGAGGACGAUGCGGU UAG UCGAGGCCACUUGUGCCAGACGACUCGCCCGA 1760_ H32
TAAAGGTGAGGGTCGCTGTC GGGAGGACGAUGCGGUAAAGGUGAGGGUCGCUC UCCAGACGACUCGCCCGA 1761_ H32
ACCCTCCACAGTCGTTTGGT GGGAGGACGAUGCGGACCCUCCACAGUCGUU UGGUCAGACGACUCGCCCGA 1762_ H32
CCGTTCGCTTATTCTCCCCC GGGAGGACGAUGCGGCCGUUCGCUUAUUCUCCCCCCAGACGACUCGCCCGA 1763 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TGACTTCCCAGAACCATCGC GGGAGGACGAUGCGGUGAC'JUCCCAGAACCAUCGCCAGACGACUCGCCCGA 1764_ H32
GCCTTCGTTTTGGTTCGTCCC GGGAGGACGAUGCGGGCCU UCGUUUUGGUUCGUCCCCAGACGACUCGCCCGA 1765_ H32
CCG CACATTCCTCCGTGGTA GGGAGGACGAUGCGGCCGCACAU UCCUCCGUGG UACAGACGACUCGCCCGA 1766_ H32
TATA6GAGGTGTAGGGACCG GGGAGGACGAUGCGGUAUAGGAGGUGUAQGGACGGCAGACGACUCGCCCGA 1767_ H32
CTCCGAATTCCCCGTCTGCC GGGAGGACGAUGCGGCUCCGAAUUCCCCGUCUGCCCAGACGACUCGCCCGA 1768_ H32
CGCCATTGTGTTGGTGTGTG GGGAGGACGAUGCGGGGCCAUUGUGUUGGUGUGUGCAGACGACUCGCCCGA 1769_ H32
AAAAGGGATGACTCGTGGTG GGGAGGACGAUGCGGAAAA.GGGAUGACUCGUGGUGCAGACGACUCGCCCGA 1770_ H32
TCAGTCGTACTGAGTCTGTC GGGAGGACGAUGCGGUCAGUCGUACUGAG UCUGUCCAGACGACUCGCCCGA 1771_ _H32
AAGTCCATCGATCGTTG GTG GGGAGGACGAUGCGGAAGUCCAUCGAUCGUUGGUGCAGACGACUCGCCCGA 1772_ H32
AAT6AGGAGGAGGTGGTGCG GGGAGGACGAUGCGGAAUGAGGAGGAGG U6GU6CGCAGACGACUC.GCCCGA 1773_ .H32
CTTAGTTAATTGTTGTTGTG GGGAGGACGAUGCGGCUUAGU UAAUUGUUGUUGUGCAGACGACUCGC CGA 1774_ .H32
GCTGTTTCCTCTGTTCCCAC GGGAGGACGAUGCGGGCUGUU UCCUCUGUUCCCACCAGACGACUCGCCCGA 1775_ _H32
TG C CG AATG TCCTGTG ACCC GGGAGGACGAUGCGGUGCCGAAUGUCCUGUGACCCCAGACGACUCGCCCGA 1776_ H32
ATCGCTGTTCTCCATGTCCT GGGAGGACGAUGCGGAUCGC'JG UUCUCCAUGUCCUCAGACGACUCGCCCGA 1777_ H32
ATCCTCTGATCGTCGATCGC GGGAGGACGAUGCGGAUCCUCUGAUCGUCGAUCGCCAGACGACUCGCCCGA 1778_ H32
ATTGACTACCCTTACCGCGT GGGAGGACGAUGCGGAUUGACUACCCU UACCG GUCAGACGACUCGCCGGA 1779^ ^H32
TCCTTGAGTTG GTTAGGCCC GGGAGGACGAUGCGGUCCUUGAGUUGGUUAGGCCCCAGACGACUCGCCCGA 1780_ ,H32
TGCCGTTTGTAACTGGTCTG GGGAGGACGAUGCGGUGCCGUUUGUAACUGGUCUGCAGACGACUCGCCCGA 1781_ _H32
GTGACTTGTCCTGTG.TTGTG GGGAGGACGAUGCGGG UGACU UGUCCUGUGUUG UGCAGACGACUCGCCCGA 17S2_ .H32
TGACA ACTCCCCTG CAACTG GGGAGGACGAUGCGGUGACAACUCCCCUGCAACUGCAGACGACUCGCCCGA 17S3_ _H32
TTCTACG TCTG TC CC G G TG GGGAGGACGAUGCGGU UCUACGUCUGUCCCGGUGCAGACGACUCGCCCGA 1784_ .H32
TATCCGGGAGGAACGGTGTG GGGAGGACGAUGCGGUAUCCGGGAGGAACGGUGUGCAGACGACUCGCCCGA 1785. ,H32
GCATTGTGATGGTGCGGCTG GGGAGGACGAUGCGGGCAUUG UGAUGGUGCGGCUGCAGACGACUCGCCCGA 1786, „H32
ITG AATG G GTAACACTG GTG GGGAGGACGAUGCGGU UGAAUGGGUAACACUGGU6CAGACGACUCGCCCGA 1787_ _H32
CGTACTCGTCTATTCCCCCC GGGAGGACGAUGCGGCGUACUCGUCUAUUCCCCCCCAGACGACUCGCCCGA 1788. .H32
ATCCTG TCGTCTGTTCGTCCC GGGAGGACGAUGCGGAUCCUGUCGUCUGUUCG JCCCCAGACGACUCGCCCGA 1789. .H32
TTGGGTGTAGGGAGTTCCG GGGAGGACGAUGCGGU UGGGUGUAGGGAGU UCCGCAGACGACUCGCCCGA 1790_ _H32
ATTGGTG AG G GAGTACCG GGGAGGACGAUGCGGAUUGGUGAAGGGAGUACCGCAGACGACUCGCCCGA 1791 H32
HTS read variable region NA sequences in H3 selection selection Selected sequenc
CTTGAAGGGTGATGGGCCCG GGGAGGACGAUGCGGCUUGAAGGGUGAUGGGCCCGCAGACGACUCGCCCGA 1792, „H32 4823 H04
ACTTGTGGTTATGACCCC6C GGGAGGACGAUGCGGACUUGUGGUUAUGACCCCGCCAGACGACUCGCCCGA 1793_ _H32
CCTTGTTTCGTCCTGCTGGC GGGAGGACGAUGCGGCCUUGUUUCGUCCUGCUGGCCAGACGACUCGCCCGA 1794. _H32
TATAACTCATGTTGTCTGGC GGGAGGACGAUGCGGUAUAACUCAUGUUGUCUGGCCAGACGACUCGCCCGA 1795. H32
CCCGTGCATCGTGTACTGT6 GGGAGGACGAUGCGGCCCGUGCAUCGUGUACUG JGCAGACGACUCGCCCGA 1796. _H32
TTTAAAGGGGGATGGGTCCG GGGAGGACGAUGCGGU UUAAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 1797. _H32 3524 H04
TTCCTTTCGCGCGTTGTCTG GGGAGGACGAUGCGGU UCCU UUCGCGCGU UG UCUGCAGACGACUCGCCCGA 1798. _H32
AGCCGTAGTAGTCTGTTGCC GGGAGGACGAUGCGGAGCCG UAGUAGUCUGUUGCCCAGACGACUCGCCCGA 1799. .H32
TCTTTCTCGTCTCCTGGCGT GGGAGGACGAUGCGGUCUUUCUCG UCUCCUGGCGUCAGACGACUCGCCCGA 1800. .Ή32
TGAGCGTCAGCTGTTCGCGC GGGAGGACGAUGCGGUGAGCGUCAGCUGUUCGCGCCAGACGACUCGCCCGA 1801. H32
CAAAACGTCCGTTCTGGCTG GGGAGGACGAUGCGGCAAAACGUCCGUUCUGGCUGCAGACGACUCGCCCGA 1802. _H32
TG TCTTCCCTG CGG G TTGTC GGGAGGACGAUGCGGUGUCUUCCCUGCGGGU UGUCCAGACGACUCGCCCGA 1803. H32
CGTAATGCAGTCTGTGGCTG GGGAGGACGAUGCGGCGUAAUGCAG UCUG UGGCUGCAGACGACUCGCCCGA 1804. _H32
TCATCTAGGTCTCACTGGCC GGGAGGACGAUGCGGUCAUCUAGGUCUCACUGGCCCAGACGACUCGCCCGA 1805. _H32
TTTCGTCGTGCCGCCGTGTG GGGAGGACGAUGCGGUUUCGUCGUGCCGCCGUGUGCAGACGACUCGCCCGA 1806. _H32
CGATG AATTG ACGTGTTGTG GGGAGGACGAUGCGGCGAUGAAUUGACGUGUUG UGCAGACGACUCGCCCGA 1807. .H32
CCAGTAGTCTGTTCAGTGTG GGGAGGACGAUGCGGCCAGUAGUCUGUUCAGUGUGCAGACGACUCGCCCGA 1808. .H32
ACAGTCGGGATCTGTGTGGC GGGAGGACGAUGCGGACAGUCGGGAUCUG UGUGGCCAGACGACUCGCCCGA 1809. .H32
rrrc A AA AG c GTA ATTGC TG GGGAGGACGAUGCGGUUUCAAAAGCGUAAUUGCUGCAGACGACUCGCCCGA 1810. .H32
ATTCGCTCCCATCCGTTCGC GGGAGGACGAUGCGGAUUCGCUCCCAUCCGU UCGCCAGACGACUCGCCCGA 1811. H32
TTGGGTGTAGGGGTGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGGUGG UACCGCAGACGACUCGCCCGA 1812. „H32
GTAAACAGTGGTCGCCCCTC GGGAGGACGAUGCGGGUAAACAGUCGUCGCCCCUCCAGACGACUCGCCCGA 1813. H32
TCGATGTCGAGTTACATCCG GGGAGGACGAUGCGGUCGAUGUCGAGUUACAUCCGCAGACGACUCGCC GA 1814. .H32
CCTTACTGACTCCCGGTTGC GGGAGGACGAUGCGGCCUUACUGACUCCCGGUUGCCAGACGACUCGCCCGA 1815. .H32
TTCGGGTCTGTTCGCCTGTG GGGAGGACGAUGCGGUUCGGG UCUGUUCGCCUGUGCAGACGACUCGCCCGA 1816. H32
TTTGAAGGGTGATAGGTACTG GGGAGGACGAUGCGGUUUGAAGGGUGAUAGGUACUGCAGACGACUCGCCCGA 1817. „H32
ATTCGTCACTCTGCTGCTGC GGGAGGACGAUGCGGAUUCGUCACUCUGCUGCUGCCAGACGACUCGCCCGA 1818. .H32
GTCCTGTCGTCTGTTCGCCCC GGGAGGACGAUGCGGG UCCUGUCGUCUGUUCGCCCCCAGACGACUCGCCCGA 1819 H32 3167 H04
ff ίη Η4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc
TTGGGAG6G6GATGGTACCG GGGAGGACGAUGCGCU UGGGAGGGGGAUGGUACCGCAGACGACUCGCCCGA 1820_ .H32
CA6AGGAGAAGGGAGTTTGC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGUULGCCAGACGACUCGCCCGA 1821. .H32
CTTGTCGTCTTGTTCGTCCCC GGGAGGACGAUGCGGCUUGUCGUCUUGUUCG UCCCCCAGACGACUCGCCCGA 1822. _H32
ATTG G CG AAGG GAG G G ACTG GGGAGGACGAUGCGGAUUGGCGAAGGGAGGGACUGCAGACGACUCGCCCGA 1823. .H32
TTTG AAG G GTG GTGG GTCCA GGGAGGACGAUGCGGUUUGAAGGGUGG UGGGUCCACAGACGACUCGCCCGA 1824. .H32 4458. .H04
TTTGAAGGGTGGTGGGTACG GGGAGGACGAUGCGGUUUGAAGGGUGG UGGGUACGCAGACGACUCGCCCGA 1825, _H32
TTGGGTGGGGGGTGGGACCG GGGAGGACGAUGCGGUUGSGUGGGGGGUGGGACCGCAGACGACUCGCCCGA 1826. .H32
TATACGGCGGTGTGGGGGTA GGGAGGACGAUGCGGUAUACGGCGGUGUGGGGGUACAGACGACUCGCCCGA 1827. .H32
ATTGGTG AAGG GTG G GACCG GGGAGGACGAUGCGGAUUGGUGAAGGGUGGGACCGCAGACGACUCGCCCGA 1828_ .H32
TTTGAAGTTTGATGGGTCCG GGGA6GACGAUGCGGUUUGAAGUUUGAUGGGUCCGCAGACGACUCGCCCGA 1829 , _H32 4070. _H04
TG ACTTGTCTG TTTCG TCCC GGGAGGACGAUGCGGUGACUUGUCUGUUUCGUCCCCAGACGACUCGCCCGA 1830. _H32
TTTGAAGGGAGATGCGTCCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGCG UCCGCAGACGACUCGCCCGA 1831. H32 3101_ _H04
TTTGAAGGGGGTGGGTCCG G6GAGGACGAUGCGGUUUGAAGGGGGUGGGUCCGCAGACGACUCGCCCGA 1832. H32 2845_ _H04
GTAATTGTGTG AG CCGTCCC GGGAGGACGAUGCGGG UAAUUGUGUGAGCCGUCCCCAGACGACUCGCCCGA 1833. .H32
TGTA6GGAGGTAGTTATGT GGGAGGACGAUGCGGUGUAGGGAGGUAGUUAUG UCAGACGACUCGCCCGA 1834_ „H32
CTTGTCGCCTTGTTCGTCCC GGGAGGACGAUGCGGCUUGUCGCCUUGUUCGUCCCCAGACGACUCGCCCGA 1835. .H32
TTGGAAGGGTGATGGGACCG GGGAGGACGAUGCGGU UGGAAGGGUGAUGGGACCGCAGACGACUCGCCCGA 1836. .H32
CGGTGTAGGGT6GTACCG GGGAGGACGAUGCGGCGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 1837. _H32
CTTGTCG TCCTG TTCGTCCC GGGAGGACGAUGCGGCUUGUCGUCCUGUUCGUCCCCAGACGACUCGCCCGA 1838. _H32 4496. _H04
ATTGGTGAAGGAAGGGACTG GGGAGGACGAUGCGGAUUGGUGAAGGAAGGGACUGCAGACGACUC6CCCGA 1839. H32
TTTGACGGGTGATGGGCCC6 GGGAGGACGAUGCGGUUUGACGGG UGAUGGGCCCGCAGACGACUCGCCCGA 1840 H32 4376. K04
CGTTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGCGUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 1841. ,H32
TCCGTAACCTCCCCGTGCTG GGGAGGACGAUGCGGUCCGUAACCUCCCCGUGCUGCAGACGACUCGCCCGA 1842. _H32
TGTAGGGGGAGGAGGGACCG GGGAGGACGAUGCGGLIGUAGG6GGAGGAGGGACC6CAGACGACUCGCCCGA 1843. _H32
TTTGAAGGAGGGTGGTACCG GGGAGGACGAUGCGGUUUGAAGGAG6GUGGUACCGCAGACGACUCGCCCGA 1844. _H32
CTGATCGTTTGGGTGTG CCC GGGAGGAC6AUGCGGCUGAUCG UUUGGG UGUGCCCCAGACGACUCGCCCGA 1845. _H32
TTTGATGGGTGTTGGGTCCG GGGAGGACGAUGCGGUUUGAUGG6UGUUGGGUCCGCAGACGACUCGCCCGA 1846. ,H32 4370. _H04
TT'GGGTGCAGGGGGGTACCG GGGAGGACGAUGCGGUUGGGUGCAGGGGGG UACCGCAGACGAC.UCGCCCGA 1847 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TG G6T6TAG G GTG TTACCG GGGAGGACGAUGCGG UGGGUGUAGGG UGU UACCGCAGACGACUCGCCCGA 184S_ .H32
TTGGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGG U UGGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 1849 . „H32
TTTGAAG GGCGAAG GGTCCG GGGAGGACGAUGCGGU UUGAAGGGCGAAGGGUCCGCAGACGACUCGCCCGA 1850. H32 3646. _HG4
TTGGGCGTAGGGTGGGACCG GGGAGGACGAUGCGG UUGGGCGUAGGG UGGGACCGCAGACGACUCGCCCGA 18S1_ H32
CTTG A AG G GTG ATG G GTCTG GGGAGGACGAUGCGGCUUGAAGGG UGAUGGGUCUGCAGACGACUCGCCCGA 1852_ H32 5137. _H04
TTTG A AGG GTG ATTG G GTCCT GGGAGGACGAUGCGGUUUGAAGGGUGAUUGGGUCCUCAGACGACUCGCCCGA 1853„ H32 3113. _HG4
TTTGGAGGGTGATGCGTCTG GGGAGGACGAUGCGG UUUGGAGGGUGAUGGGUCUGCAGACGACUCGCCCGA 1854„ H32
TTTGAAGGGGGATGGGTCCT GGGAGGACGAUGCGG UUUGAAGGGGGAUGGGUCCUCAGACGACUCGCCCGA 1855_ .H32 5152_ ^H0
TTCGAAGGGTGATGGGTCCC GGGAGGACGAUGCGGUUCGAAGGG UGAUGGGUCCCCAGACGACUCGCCCGA 1856_ .H32 2080. .H04
TACTCAGTCGTCTTATACTG GGGAGGACGAUGCGGUACUCAG UCGUCUUAUACJGCAGACGACUCGCCCGA 1857_ .H32
GTAGAATCGTCGCGGCTGCC GGGAGGACGAUGCGGG UAGAAUCGUCGCGGCUGCCCAGACGACUCGCCCGA 1858_ _H32
TCCTGGCCTGTCGTTGTCCC GGGAGGACGAUGCGGliCCUGGCCUGUCGU UGUCCCCAGACGACUCGCCCGA 1859_ ,H32
ATATAGTGTGAGCCGTTTGC GGGAGGACGAUGCGGAUAUAG UG UGAGCCGU UUGCCAGACGACUCGCCCGA 1860_ H32
AAGACCGAATGTGTGTGCTG GGGAGGACGAUGCGGAAGACCGAAUG UGUGUGCUGCAGACGACUCGCCCGA 186 . .H32
TTTGAAGGTTGGTGGGTCCG GGGAGGACGAUGCGGU UUGAAGGUUGGUGGGUCCGCAGACGACUCGCCCGA 1862_ .H32 4575. _H04
ATGACAATTGGGTACTGGTG GGGAGGACGAUGCGGAUGACAAUUGGGUACUGGUGCAGACGACUCGCCCGA 1863_ H32
GATTCGATCTGTCACCTCCC GGGAGGACGAUGCGGGAU UCGAUCUGUCACCUCCCCAGACGACUCGCCCGA 1864_ H32
GTCGTTGTCAACCGTATCCC GGGAGGACGAUGCGGG UCG UUG UCAACCG UAUCCCCAGACGACUCGCCCGA 1865_ .H32
TTCGACTAGTAGTTGGTCAC GGGAGGACGAUGCGGUUCGACUAGUAGUUGG UCACCAGACGACUCGCCCGA 1866_ H32
GCAAGTTGTTGCTACCCCCC GGGAGGACGAUGCGGGCAAGUUGU UGCUACCCCCCCAGACGACUCGCCCGA 1S67_ .H32
CCGTATCTAACCTCCATGTG GGGAGGACGAUGCGGCCGUAUCUAACCUCCAUGUGCAGACGACUCGCCCGA 1868, ,H32
CAT AG C G TTT GGCGATGGGC GGGAGGACGAUGCGGCAUAGCG UU UGGCGAUGGGCCAGACGACUCGCCCGA 1869.. .H32
rCGGTAATCTTGTGTAGCTG GGGAGGACGAUGCGG UCGG UAAUCU UGUGUAGCUGCAGACGACUCGCCCGA 1870. .H32
GCCTTTCGTTCTGCTCCCCC GGGAGGACGAUGCGGGCCU U UCGUUC UGCUCCCCCCAGACGACUCGCCCGA 1871_ .H32 2491. _ H04
AGGTGTGATGAATATGGCCC GGGAGGACGAUGCGGAGG UGUGAUGAAUAUGGCCCCAGACGACUCGCCCGA 1872_ _H32
TCGGAGAGCTAACAACACCG GGGAGGACGAUGCGGUCGGAGAGCUAACAACACCGCAGACGACUCGCCCGA 1873_ „H32
GAACAACAAGTCGTCACGGC GGGAGGACGAUGCGGGAACAACAAG UCGUCACGGCCAGACGACUC6CCCGA 1.874_ ,H32
TTGGGTGAAGGGGGGTACC6 GGGAGGAOSAUGCGGU UGGGUGAAGGGGGG UACCGCAGACGACUCGCCCGA 1875 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTGGAGAAGGGTGGTACTG GGGAGGACGAUGCGGU UUGGAGAAGGGUGGUACUGCAGACGACUCGCCCGA 1876_ _H32
CATCTAACCCAGTGTGTGCC GGGAGGACGAUGCGGCAUCUAACCCAGUGUGUGCCCAGACGACUCGCCCGA 1877. .H32
ACGAAGATCGATGCTGTGCC GGGAGGACGAUGCGGACGAAGAUCGAUGCUGUGCCCAGACGACUCGCCCGA 1878_ _H32
GAATAGTCGGAATGCCGCGC GGGAGGACGAUGCGGGAAUAGUCGGAAUGCCGCGCCAGACGACUCGCCCGA 1879_ .H32
AGAGCGATGTCGGTTTGGCC GGGAGGACGAUGCGGAGAGCGAUGUCGGU UUGGCCCAGACGACUCGCCCGA 1S80. .H32
TCACCGTTTCCTCCTGTGCC GGGAGGACGAUGCGGUCACCGUU UCCUCCUGUGCCCAGACGACUCGCCCGA 1881. _H32
CTAG i CAGG TGTTTG GG CGC GGGAGGACGAUGCGGCUAGUCAGGUGUUUGGGCGCCAGACGACUCGCCCGA 1882_ H32
CACCATAACCGTGCGGTCTG GGGAGGACGAU6CGGCACCAUAACCGUGCGGUCUGCAGACGACUCGCCCGA 1883. .H32
AATATGGCGCCCTTCACGTG GGGAGGACGAUGCGGAAUAUGGCGCCCUUCACGUGCAGACGACUCGCCCGA 1884. H32
ATCAGTT AACG CACAG CCTG GGGAGGACGAUGCGGAUCAGUUAACGCACAGCCUGCAGACGACUCGCCCGA 1885. _H32
GTAAGAGGTCAATGCTTGGC GGGAGGACGAUGCGGGUAAGAGGUCAAUGCUUGGCCAGACGACUCGCCCGA 1886. H32
TCCTCGG 6TATCG ATCTGTG GGGAGGACGAUGCGGUCCUCGGGUAUCGAUCUGUGCAGACGACUCGCCCGA 1887_ H32
GCCTTTC6T CTTCCTCCC GGGAGGACGAUGCGGGCC UUUCGUUCUUCCUCCCCAGACGACUCGCCCGA 1888_ H32 2569 H04
GAAGCGCCTGACTATGTGCC GGGAGGACGAUGCGGGAAGCGCCUGACUAUGUGCCCAGAGGA UCGCCCGA 1889. H32
AATTGTTCATTCTACATG CC GGGAGGACGAUGCGGAAUUG UUCAUUCUACAUGCCCAGACGACUCGCCCGA 1890_ H32
GTG ATTGTACGTCTTCTCCC GGGAGGACGAUGCGGGUGAUUGUACGUCUUCUCCCCAGACGACUCGCCCGA 1891_ H32
ATTGGTCAAGGGAGGTACCG GGGAGGACGAUGCGGAU UGGUCAAGGGAGGUACCGCAGACGACUCGCCCGA 1892_ .H32
GAGATTGCCTGCGCCTTGTG GGGAGGACGAUGCGGGAGAUUGCCUGCGCCUUGUGCAGACGACUCGCCCGA 1893. „H32
ACG CCTTATTCTG CAG GCC A GGGAGGACGAUGCGGACGCCUUAUUCUGCAGGCCACAGACGACUCGCCCGA 1894. H32
GAG TACG TG TGCCGTAG CTG GGGAGGACGAUGCGG AGi IACGUGUGCCGUAGCUGCAGACGACUCGCCCGA 1895_ H32
TATCGGTCCTAGTCCAGCGC GGGAGGACGAUGCGGUAUCGGUCCUAGUCCAGCGCCAGACGACUCGCCCGA 1896. H32
TACGTTCTCTCCTGTGGCCC GGGAGGACGAUG'CGGUACGUUCUCUCCUGUGGCCCCAGACGACUCGCCCGA 1897_ _H32
CAGTTCGCTCCCACAAGCCC GGGAGGACGAUGCGGCAGUUCGCUCCCACAAGCCCCAGACGACUCGCCCGA 1S98_ .H32
TCTGGGGAAATAACGTGCTG GGGAGGACGAUGCGGUCUGGGGAAAUAACGUGCUGCAGACGACUCGCCCGA. 1899_ H32
GTCTTCTGTCTTACCGTCCC GGGAGGACGAUGCGGGUCUUCUGUCUUACCGUCCCCAGACGACUCGCCCGA 1900_ H32
TCCCGTCCCGTGTATGTTGC GGGAGGACGAUGCGGUCCCGUCCCGUGUAUGUUGCCAGACGACUCGCCCGA 1901. H32
TAGCTTGTTCCCCGATGCCC GGGAGGACGAU6CGGUAGCU UG UUCCCCGAUGCCCCAGACGACUCGCCCGA 1902_ H32
CTCAGTTGTGTGGCT GCGC GGGAGGAC6AUGCGGCUCAGUUG UGUGGCUUGCGCCAGACGACUCGCCC.GA 1903 H32
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc
CATTCGTTTGTGCTCGTTGC GGGAGGACGAUGCGGCAUUCGUUUGU6CUCGUUGCCAGACGACUCGCCCGA 1904. .H32
TTGGGTGTAGGGTGGGACTG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGGACUGCAGACGACUCGCCCGA 1905_ _H32
CGAGCAATAACTACTGTCCC GGGAGGACGAUGCGGCGAGCAAUAACUACUGUCCCCAGACGACUCGCCCGA 19Q6. _H32
ATTCGGCACACCTTCCTCGC GGGAGGACGAUGCGGAUUCGGCACACCUUCCUCGCCAGACGACUCGCCCGA 1907„ .H32
ACG AGTCTG TG ACTTCG CGC GGGAGGACGAUGCGGACGAGUCUGUGACU UCGCGCCAGACGACUCGCCCGA 1908. H32
TTTGAAGGGTGGTGGGTCGG GGGAGGACGAUGCGGU UUGAAGGGUGG UGGGUCGGCAGACGACUCGCCCGA 1909, _H32
TCCTGTCGTCTGTTCGGCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGGCCCCAGACGACUCGCCCGA 1910_ .H32 0298 H04
TCCTGGCTTGTCGTTGTCC GGGAGGACGAUGCGGUCCUGGCUUGUCGUUGUCCCAGACGACUCGCCCGA 1911. .H32
TTCTTCCCTTGCACGTGTGC GGGAGGACGAUGCGGUUCUUCCCUUGCACGUGUGCCAGACGACUCGCCCGA 1912. H32
TTTGAAGGGTGATGGGCCTG GGGAGGACGAUGCGGUUU6AAGGGUGAUGGGCCUGCAGACGACUCGCCCGA 1913. _H32
TCCTGGCTTGTGTTGTCCC GGGAGGACGAUGCGGUCCUGGCUUGUGUUGUCCCCAGACGACUCGCCCGA 1914. .H32 3954 H04
TTG G CC AGTAGATGTTACTG G6GA6GACGAUGCGGUUGGCCAGUAGAUGUUACUGCAGACGACUCGCCCGA 1915 ^H32
TTTGAAGGGTGATGCCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGCCCGCAGACGACUCGCCCGA 1916_ H32
CCAATACGTG ATTCTGGCCC GGGAGGACGAUGC6GCCAAUACGUGAUUCUGGCCCCAGACGACUCGCCCGA 1917. _H32
GTACGTAATTTACTGTGCCC GGGAGGACGAUGCGGG UACGUAAUUUACUGUGCCCCAGACGACUCGCCCGA _H32
GTTCACCAATGACCGATCTG GGGAGGACGAUGCGGGUUCACCAAUGACCGAUCUGCAGACGACUCGCCCGA 1919. .H32
G TG CTTGTCA G G TTT A.CC G C GGGAGGACGAUGCG6G UGCUUGUCAGGU UUACCGCCAGACGACUCGCCCGA 1920. _H32
TTTGAAGGGTGATGG6GGGTG GGGAGGACGAUGCG6UUUGAAGGGUGAUGGGGGGUGCAGACGACUCGCCCGA 1921. _H32
AACATCACTCCCACTCCCCC GGGAGGACGAUGCGGAACAUCACUCCCACUCCCCCCAGACGACUCGCCCGA 1922. .H32
TATG TTG CG CTTTCTCTCCC GGGAGGACGAUGCGGUAUGUUGCGCUUUCUCUCCCCAGACGACUCGCCCGA 1923. _H32
TCACGGTATTCTGCG TGCCC GGGAGGACGAUGCGGUCACGGUAUUCUGCGUGCCCCAGACGACUCGCCCGA 1924. H32
CATAGTCGTCTTTCAGCCCC GGGAGGACGAUGCGGCAUAG UCGUCUUUCAGCCCCCAGACGACUCGCCCGA 1925, .H32
CCCTTCGTCCTACGTCTGTG GGGAGGACGAUGCGGCCGUUCGUCCUACGUCUG UGCAGACGACUCGCCCGA 1926. .H32
GATTGGGCTGATCCTGTGTG GGGAGGACGAUGCGGGAUUGGGCUGAUCCUGUGUGCAGACGACUCGCCCGA 1927„ _H32
TCTCGTTACTCCCGTCCCCC GGGAGGACGAUGCGGUCUCGUUACUCCCGUCCCCCCAGACGACUCGCCCGA 1928. _H32
CGTAGTCATCGACACGTGTA GGGAGGACGAUGCGGCGUAG UCAUCGACACGUGUACAGACGAf UCGtrCGA 1929. _H32
AG AAGTCTGTATTG CG TG GC GGGAGGACGAUGCGGAGAAGUCUGUAUUGCG UoGCCAGACGACUCGCCCGA 1930. _H32
AAAT6GCTTACTGTGTGCTG GGGAGGACGAUGCGGAAAUGGCUUACUGUGUGCUGCAGACGACUCGCCCGA 1931. H32
# in H4
HTS read variable region NA sequences 8 in H3 selection selection Selected sequenc
TAG G TCGCTGCATG ACTGTG GGGAGGACGAUGCGGUAGGUCGCUGCAUGACUGU6CAGACGACUCGCCCGA 1932_H32
TTTGGGGTTTCCCCCTGCCC GGGAGGACGAUGCGGUUUGGGGUU UCCCCCUGCCCCAGACGACUCGCCCGA 1933_H32
Τ6εα 6ϋ6ΤΤΤ06ΤΤΤ0 GGGAGGACGAUGCGGUGCGUUGCGUUUGGUUUGCAGACGACUCGCCCGA 1934_H32
GTTG AAG G GTG AG G GGTCCG GGGAGGACGAUGCGGG UUGAAGGG UGAGGGGUCCGCAGACGACUCGCCCGA 1935_H32
TG GTTACTCTCTACG CTCCG GGGAGGACGAUGCGGUGGUUACUCUCUACGCUCCGCAGACGACUCGCCCGA 1936_H32
GTCATTGTTTTTGTCGTGTG GGGA6GACGAUGC6GGUCAUUGUUU UUGUCGUGUGCAGACGACUCGCCCGA 1937_H32
AATCTACCTTCCTGTC G TGC GGGAGGACGAUGCGGAAUCUACCUUCCUGUCGUGCCAGACGACUCGCCCGA I938_H32
TCTTCCAAACG GTCCGTGTG GGGAGGACGAUGCGGUCUUCCAAACGGUCC UG UGCAGACGACUCGCCCGA 1939„H32
ATTGGAGGGGGATGGGACTG GGGAGGACGAUGCGGAUUGGAGGGGGAUGGGACUGCAGACGACUCGCCCGA 1940_H32
GCCTTG CGTTCTG ACCTCCC GGGAGGACGAUGCGGGCCUUGCGUUCUGACCUCCCCAGACGACUCGCCCGA 1941„H32
TCGATCGTAATGTCTGGCCC GGGAGGACGAUGCGGUCGAUCGUAAUGUCUGGCCCCAGACGACUCGCCCGA 1942_ H32
CTAGTCGAACCGATGCTGTC GGGAGGACGAUGCGGCUAGUCGAACCGAUGCUG UCCAGACGACUCGCCCGA 1943_H32
ATGAGGTAAGGACCACCGTG GGGAGGACGAUGCGGAUGAGGUAAGGACCACCGUGCAGACGACUCGCCCGA 1944_H32
TCGATGTCTTCCACGTGGCC GGGAGGACGAUGCGGUCGAUG IJCLJU CACGUGGCCCAGACGACUCGCCCGA 1945„H32
ATTG CTCAGG CTGTGGTGGC GGGAGGACGAUGCGGAUUGCUCAGGCUGUGG UGGCCAGACGACUCGCCCGA 1946_ H32
TTTGTCGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUG UCGGGUGAUGGGUCCGCAGACGACUCGCCCGA 1947_H32 3845JH04
1TIG AAGGG CGACG G 6TCCG GGGAGGACGAUGCGGUUUGAAGGGCGACGGGUCCGCAGACGACUCGCCCG . 1948_H32 3G54_H04
TTTGAAGGGTGATATGTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUAUGUCCGCAGACGACUCGCCCGA 1949_H32 4777 H04
TATA CGG CG GTG G G G G GAT A GGGAGGACGAUGCGGUAUACGGCGG UGGGGGGAUACAGACGACUCGCCCGA 1950_H32
TCTTGTCG TCTCTTCGTCCC GGGAGGACGAUGCGGUCUUGUCGUCUCUUCGUCCCCAGACGACUCGCCCGA 1951_ B32
TTTGA A GGGGGATAG GTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUAGGUCCGCAGACGACUCGCCCGA 1952_ H32 4019 H04
ATTCCACATTGTCGCGTCCC GGGAGGACGAUGCGGAUUCCACAUUGUCGCGUCCCCAGACGACUCGCCCGA 1953_H32
ATATCGCCTCCTAACGTGCC GGGAGGACGAUGCGGAUAUCGCCUCCUAACGUGCCCAGACGACUCGCCCGA 1954_H32
TTTGAAGGGCGATGGGTCTG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGUCUGCAGACGACUCGCCCGA 1955_H32
ACAAATCGGCTGGCGTGCAC GGGAGGACGAUGCGGACAAAUCGGCUGGCGUGCACCAGACGACUCGCCCGA 1956_H32
GACGCTACACGGGGTTTGTG GGGAGGACGAUGCGGGACGCUACACGCGGU UUG JGCACACGACUCGCCCGA 1957__H32
TTTGAGGGGTGATGGGACCG GGGAGGACGAUGCGGUUUGAGGGGUGAUGGGACCGCAGACGACUCGCCCGA 1958_H32
TACCGCACCTCTACGATCCG GGGAGGACGAUGCGGUACCGCACCUCUACGAUCCGCAGACGACUCGCCCGA 1959 H32
# ir» H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CGTC6 A 6 CTTCCTG TCTG GGGAGGACGAUGCGGCGUCGAGCUUCCUGUCUGCAGACGACUCGCCCGA I960.. .H32
GATGACGTTGTCGTGGCCTC GGGAGGACGAUGCGGGAUGACGU UGUCGUGGCCUCCAGACGACUCGCCCGA 1961_ H32
AACCAGTAATACCCGCTCCC GGGAGGACGAUGCGGAACCAGUAAUACCCGCUCCCCAGACGACUCGCCCGA 1962_ .H3-2
ATTGGTG A AGG GAG G G TTG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGGU UGCAGACGACUCGCCCGA 1963_ H32
CATCG CACTGTTACTGTCCC GGGAGGACGAUGCGGCAUCGCACUGUUACUGUCCCCAGACGACUCGCCCGA 1964_ _H32
TTTGAAGGGTGATGGGTCTC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCUCCAGACGACUCGCCCGA 1965„ .H32 4503 H04
TCCTCAACG ATTCG CTG CGT GGGAGGACGAUGCGGUCCUCAACGAUUCGCUGCGUCAGACGACUCGCCCGA 1966_ ,H32
ACATACCGATCTCCCGTCCC GGGAGGACGAUGCGGACAUACCGAUCUCCCGUCCCCAGACGACUCGCCCGA 1967_ .H32
ATTCGGCTTCCTGTGTGCAC G6GAGGACGAUGCGGAUUCGGCUUCCUGUGUGCACCAGACGACUCGCCCGA 1968_ .H32
TATCGGTGTCCCACGGTCGC GGGAGGACGAU6CGGUAUCGGUGUCCCACGGUCGCCAGAC6ACUCGCCCGA 1969_ .H32
GTACT ACT G G C ATG CG G AC GGGAGGACGAU6CGGG UACUACUUGGCAUGCGGACCAGACGACUCGCCCGA 1970_ H32
CCTTTGTCTGGTGCCGTTAC GGGAGGACGAUGCGGCCUUUGUCUGGUGCCG UUACCAGACGACUCGCCCGA 1971_ mi
GAATTCGCTTGGCTAGTGGC GGGAGGACGAUGCGGGAAUUCGCUUGGCUAGUGGCCAGACGACUCGCCCGA 1S72_ .H32
TTTG AAG GG G G GTG GG TCTG GGGAGGACGAUGCGGU UUGAAGGGGGG UGGGUCUGCAGACGACUCGCCCGA 1973. .H32
AG AGTTG TA ACTG TG GTG TG GGGAGGACGAUGCGGAGAGUUGUAACUGUGGUG UGCAGACGACUCGCCCGA 1974_ .M32
TTCGCAATGCC TTCCGCCC GGGAGGACGAUGCGGU UCGCAAUGCCUUUCCGCCCCAGACGACUCGCCCGA 1975_ .H32
TGAGTTCTATCTGTTGGCCC GGGAGGACGAUGCGGUGAG UUCUAUCUGUUGGCCCCAGACGACUCGCCCGA 1976_ „H32
GTTGAAGGGTGATGGGACCG GGGAGGACGAUGCGGGUUGAAGGGUGAUGGGACCGCAGACGACUCGCCCGA 1977_ _H32
TCTAG G GTCCTCC G TTGCTG GGGAGGACGAUGCGGUCUAGGGUCCUCCGUUGCUGCAGACGACUCGCCCGA 197S_ _H32
CAAATACCTTCCACCCGCTG GGGAGGACGAUGCGGCAAAUACCUUCCACCCGCUGCAGACGACUCGCCCGA 1979_ _H32
CCGTCTCTTGACGTGCTCCC GGGAGGACGAUGCGGCCGUCUCUUGACGUGCUCCCCAGACGACUCGCCCGA 1980_ .H32
TTGGGTGTATGGAGGTACCG GGGAGGACGAUGCGGU UGGGUGUAUGGAGGUACCGCAGACGACUCGCCCGA 1981. H32
TGGTGGAGTTTGTCTGTGTG GGGAGGACGAUGCGGUGG U6GAGUUUGUCUGUGUGCAGACGACUCGCCCGA 1982. _H32
TTTGAGGG GTG ATGGGG CCG GGGAGGACGAUGCGGUUUGAGGGGUGAUGGGGCCGCAGACGACUCGCCCGA 1983_ _H32 4968_H04
GTTGAAGGGTGTTGGGTCCG G6GAGGACGAUGCGGGUUGAAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 1984_ _H32 38S9_H04
TTGGGTGGAGGGAGGTACCG GGGAGGACGAUGC6GUUGGGUGGAGGGAGGUACCGCAGACGACUCGCCCGA 1985_ .H32
CTGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGCUGAAGGGUGAUGGG UCCGCAGACGACUCGCCCGA 1986„ .H32 4S97 H04
TTTGGAGGATGATGGGACCG GGGAGGACGAUGCGGUUUGGAGGAUGAUGGGACCGCAGACGACUCGCCCGA 1987 H32
# in H4
HIS reed variable region RNA sequences # in H3 selection selection Selected sequenc
TTTGGTGTAGGGTGTTACCG GGGAGGACGAUGCGGU UUGG UGUAGGGUGUUACCGCAGACGACUCGCCCG 1988. H32
ATTG GGAAGGGAG GTACCG GGGAGGACGAUGCGGAUUGGGAAGGGAGGUACCGCAGACGACUCGCCCGA 1989. H32
ACGGTTGCTTTGGCGTTGGC GGGAGGACGALIGCGGACGGUUGCUUUGGCGUUGGCCAGACGACUCGCCCGA 1990. H32
CCGCAACCTTAATCCTGCCC GGGAGGACGAUGCGGCCGCAACCUUAAUCCUGCCCCAGACGACUCGCCCGA 1991_ .H32
TTTTGG GTG ATGGGTCCG GGGAGGACGAUGCGGU UUUGGGUGAUGGGUCCGCAGACGACUC6CCCGA 1.992. _H32
CTTGAAAGGTGATGGGTCCG GGGAGGACGAUGCGGCUUGAAAGG UGAUGGGUCCGCAGACGACUCGCCCGA 1993. H32
GGGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGGGGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 1994 . .H32
TTTGGAGGGGGGAGGGACCG GGGAGGACGAUGCGGUUUGGAGGGGGGAGGGACCGCAGACGACUCGCCCGA 1995_ H32
AATTGGAGGTGTAGGGACCG GGGAGGACGAUGCGGAAUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 1996. _H32
TATTAGAGGTG TAGGG ACCG GGGAGGACGAUGCGGUAUUAGAGG UGUAGGGACCGCAGACGACUCGCCCGA 1997. _H32
TTTG AAGG GTG AT3G GCCCC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGCCCCCAGACGACUCGCCCGA 199'8_ H32 2579 H04
GTCTTCTGGC7TATCGTCCA GGGAGGACGAUGCGGGUCU UCUGGCUUAUCGUCCACAGACGACUCGCCCGA 1999. .H32
TCTTCCGTAATAGCGCTGCC GGGAGGACGAUGCGGUCUUCCGUAAUAGCGCUGCCCAGACGACUCGCCCGA 20Q0_ H32
ο GCCTTCGTTACTCTGCACTG GGGAGGACGAUGCGGGCCU UCGUUACUCUGCACUGCAGACGACUCGCCCGA 2001_ .H32
CTAAGTGTCCTGCCGGTCTG GGGAGGACGAUGCGGCUAAGU6UCCUGCCGGUCUGCAGACGACUCGCCCGA 2002_ JH32
A C C CTC AT G ATCTTG G CC T G GGGAGGACGAUGCGGACCCUCAUGAUCUUGGCCUGCAGACGACUCGCCCGA 2003,. .H32
TTCACCTTCTGATCGT6CCC GGGAGGACGAUGCGGUUCACCUUCUGAUCGUGCCCCAGACGACUCGCCCGA 2004. .H32
ATGAGTCGAGTTGGGGGCTG GGGAGGACGAUGCG6AUGAGUCGAGUUGGGGGCUGCA6ACGACUCGCCCGA 200S_ .H32
TTTGTTCCCCCAATTGTGCC GGGAGGACGAUGCGGUUUGUUCCCCCAAUUGUGCCCAGACGACUCGCCCGA 2006_ H32
TG ATTGTCTG CTG G CTTG CC GGGAGGACGAUGCGGUGAUUG UCUGCUGGCU UGCCCAGACGACUCGCCCGA 2G07. .H32
TG CG ATCAC AGTACG G CGT6 GGGAGGACGAUGCGGUGCGAUCACAGUACGGCGUGCAGACGACUCGCCCGA 2008^ 32
T ATCTTCCGTCG CCTG TCAC GGGAGGACGAUGCGGUAUCUUCCGUCGCCUGUCACCAGACGACUCGCCCGA 2009. _H32
ATTGGTGAAGGGGGGGACTG GGGAGGACGAUGCGGAUUGGUGAAGGGGGGGACUGCAGACGACUCGCCCGA 2010. .H32
ATGTCCTACCCGTCGTTGGC GGGAGGACGAUGCGGAUGUCCUACCCGUCG UUGGCCAGACGACUCGCCCGA 2011. .H32
TCG CATTCATCCCCATGCCCC GGGAGGACGAUGCGGUCGCAUUCAUCCCCAUGCCCCCAGACGACUCGCCCGA 2012. H52
TCTTCCTTGTGTGTTCGCGC GGGAGGACGAUGCGGUCUUCCU UGUGUGUUCGCGCCAGACGACUCGCCCGA 2013. _H32
TATAGTGACATGTGTTGCGT GGGAGGACGAUGCGGUAUAGUGACAUGUGUUGCGUCAGACGACUCGCCCGA 2014. H32
TTA G TC CTG 6 TCTCG TTGG C GGGAGGACGAUGCGGU UAGUCCUGG UCUCGUUC-GCCAGACGACUCGCCCGA 2015 H32
# in H
HTS read variable region NA sequences # in H3 selection selection Selected sequenc TACAGCTGACCCCGTGTCAC GGGAGGACGAUGCGGUACA.GCUGACCCCG'JG UCACCAGACGACUCGCCCGA 2016, „H32
TGCGTTTCGTCTCG CGTCC GGGAGGACGAUGCGGUGCGUUUCG UCUCGCGUCCCAGACGACUCGCCCGA 2017. _H32
TTTGGTGGAGGGTGGGACTG GGGAGGACGAUGCGGUUUGGUGGAGGGUG6GACUGCAGACGACUCGCCCGA 2018_ _H32
CCTAG G AG A ATG AG TTG C AC GGGAGGACGAUGCGGCCUAGGAGAAUGAG UUGCACCAGACGACUCGCCCGA 201S_ .H32
GTAGCGTTATGGTGCAGTGC GGGAGGACGAUGCGGG UAGCGUUAUGG UGCAGUGCCAGACGACUCGCCCGA 2020. ^H32
TCCTGTCGTCTGTTGGCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGCCCCAGACGACUCGCCCGA 2021. _H32 0514 H04
TGTCGTAGATGGTTGCCGGC GGGAGGACGAUGCGGUGUCGUAGA'JGG UUGCCGGCCAGACGACUCGCCCGA 2022, .H32
CTTTATACTGTAAGCAACG6 GGGAGGACGAUGCGGCUUUAUACUGUAAGCAACGGCAGAC6ACUCGCCCGA 2023. JH32
ACCATACCTTCTTGCCCCCC GGGAGGACGAUGCGGACCAUACCUUCUUGCCCCCCCAGACGACUCGCCCGA 2024. .H32
CCTG C AACTTTTCGCTG CTG GGGAGGACGAUGCGGCCUGCAACUUUUCGCUGCUGCAGACGACUCGCCCGA 2025. .H32
TAG G G G CAATCG TCTATG G C GGGAGGACGAUGCGGUAGGGGCAAUCGUCUAUGGCCAGACGACUCGCCCGA 2026. _H32
ACCGCTTAATAATCCTGCCC GGGAGGACGAUGCGGACCGCUUAAUAAUCCUGCCCCAGACGACUCGCCCGA 2027. _H32
TCCTAAAATCGTTACCTGCC GGGAGGACGAUGCGGUCCUAAAAUCGUUACCUGCCGAGACGACUCGCCCGA 2028. _H32
CACGTCTTCTCCGTTTGCGC GGGAGGACGAUGCGGCACGUCUUCUCCGUU UGCGCCAGACGAC.UCGCCCGA 2029. H32
ATAGGGAGAAAGGTGAACCG GGGAGGACGAUGCGGAUAGGGAGAAAGGUGAACCGCAGACGACUCGCCCGA 2030. .H32
GTAAGTT6TCAGCCTCTCGC GGGAGGACGAUGCGGG UAAGUUG UCAGCCUCUCGCCAGACGACUCGCCCGA 2031. _H32
TTTG AGTGGTGATGG GTCCG GGGAGGACGAUGCGGU UUGAG UGGUGAUGGGUCCGCAGACGACUCGCCCGA 2032. ^H32
TGTGCTTTATGCCTGGCCC GGGAGGACGAUGCGGUGUGCU UUAUGCCUGGCCCCAGACGACUCGCCCGA 2033. _H32
GTTTTCTGGCTTATCGTCCC GGGAGGACGAUGCGGG UUUUCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 2034. .H32
G G ATG ATGTTAG TTTG GCG C GGGAGGACGAU6CGGGGAUGAUGUUAGUUUGGCGCCAGACGACUCGCCCGA 2035. ^H32
GTAAGACGTCCACATACGTG GGGAGGACGAUGCGGG UAAGACGUCCACAUACGUGCAGACGACUCGCCCGA 2036. ,H32
CGTTTCCTCTGGTTCGTCCT GGGAGGACGAUGCGGCG UUUCCUCUGGUUCG UCCUCAGACGACUCGCCCGA 2037. .H32
AATCCTCAAAAGTGCGTGTA GGGAGGACGAUGCGGAAUCCUCAAAAGUGCGUGUACAGACGACUCGCCCGA 2038. .H32
ATACTGAGTCGTAAGTTGTG GGGAGGACGAUGCGGAUACUGAGUCGUAAGU UGUGCAGACGACUCGCCCGA 2039. .H32
TCGGCTCGTCTCGTGATCCC GGGAGGACGAU6CGGUCGGCUCGUCUCGUGAUCCCCAGAGGACUCGCCCGA 2040. _H32
TTGAAGGGT6ATGGGTCCC GGGAGGACGAUGCGGU UGAAGGGUGAUGGG UCCCCAGACGACUCGCCCGA 2041. .H32 1630 H04
TGGCGTTTTGAGCGGCTGTG GGGAGGACGAUGCGGUGGCGUUUUGAGCGGCUGUGCAGACGACUCGCCCGA 2042. _H32
TTTGAAGG GTGTTGG GCCCG GGGAGGACGAUGCGGU UUGAAGGG UGU UGGGCCCGCAGACGACUCGCCCGA 2043 H32 3201 H04
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
CACTTTCGTTCTG ACCTCCC GGGAGGACGAUGCGGCACUU UCGUUCUGACCUCCCCAGACGACUCCCCCGA 2044. _H32
TCCTCAGTGTTATGGTGCGC GGGAGGACGAUGCGGUCCUCAGUGUUAUGGUGCGCCAGACGACUCGCCCGA 2045_ .H32
TTGTATGTACGACGGTGCTG GGGAGGACGAUGCGGU UGUAUGUACGACGGUGCUGCAGACGACUCGCCCGA 2046_ „H32
GCTAAGACACTCATGTGCCG GGGAGGACGAUGCGGGCUAAGACACUCAUG UGCCGCAGACGACUCGCCCGA 2047_ H32
TGGTTTGACTGTCTGTGCCC 6GGAGGACGAUGCGGUGGUUUGACUGUCUGUGCCCCAGACGACUCGCCCGA 2048_ .H32
TCTG TCCTCTG TG CACTCCC GGGAGGACGAUGCGGUCUG UCCUCUGUGCACUCCCCAGACGACUCGCCCGA 2049_ H32
GATCAACTGGAACACGTGGC GGGA6GACGAUGCGGGAUCAACUGGAACACGUGGCCAGACGACUCGCCCGA 2050_ H32
TATGAAG G AAGTGCCC ACTG GGGAGGACGAUGCGGUAUGAAGGAAGUGCCCACUGCAGACGACUCGCCCGA 2051, .H32
TTTTG G TCC ATCCTGCTGCC GGGAGGACGAUGCGGUUU UGG UCCAUCCUGCUGCCCAGACGACUCGCCCGA 2052, H32
TCCTTGTCGTCTTA.CGTCCC GGGAGGACGAUGCGGUCCUUGUCGUCUUACGUC CCAGACGACUCGCCCGA 2053. H32 1862 H04
RCGTCAACAAAATGTGACGC GGGAGGACGAUGCGGUCGUCAACAAAAUGUGACGCCAGACGACUCGCCCGA 2054_ H32
TTCCCGCTAACCAGTACCCC GGGAGGACGAUGCGGU UCCCGCUAACCAGUACCCCCAGACGACUCGCCCGA 20S5. H32
TGCTGGTGGTTTCGCGGCTG GGGAGGACGAUGCGGUGCUGG UGGUUUCGCGGCUGCAGACGACUCGCCCGA 2056_ H32
GTCTACGTCGTCAGCTGCAC GGGAGGACGAUGCGGGUCUACGUCGUCAGCUGCACCAGACGACUCGCCCGA 2057_ _H32
TTCCTGTCGTCGATTTGCGC GGGAGGACGAUGCGGU UCCUGUCGUCGAUUUGCGCCAGACGACUCGCCCGA 2058. .H32
GATGAGTATCAGGCCTGCGC GGGAGGACGAUGC6GGAUGAGUAUCAGGCCUGCGCCAGACGACUCGCCCGA 2059.. .H32
TCTATA G TC AT ATCG G TG CC GGGAGGACGAUGCGGUCUAUAGUCAUAUCGGUGCCCAGACGACUCGCCCGA 2060. _H32
AG CTG G AC ACTTG G G G TCTG GGGAGGACGAUGCGGAGCUGGACACUUGGGGUCUGCAGACGACUCGCCCGA 2061. _H32
TCCGCGTTGCTCTCGCACAC GGGAGGACGAUGCGGUCCGCGUUGCUCUCGCACACCAGACGACUCGCCCGA 2062. .H32
GTTGTCCTTTGCTTCCGCAC GGGAGGACGAUGCGGGUUGUCCUUUGCUUCCGCACCAGACGACUCGCCCGA 2063. .H32
A A ATCGTC ATTTAG TG TG TC GGGAGGACGAUGCGGAAAUC6UCAUUUAGUG UGUCCAGACGACUCGCCCGA 2064. HB2
C AG AG G AG AAGG G AG GTTCC GGGAGGACGAUGCGGCAGAGGAGAAGGGAGGUUCCCAGACGACUCGCCCGA 2065. _H32
TCGTGTTCGTTTTGCATGTG GGGAGGACGAUGCGGUCGUGUUCG UUUUGCAUGUGCAGACGACUCGCCCGA 2066. .H32
TTTGAAGGGAGATGGCTCCG GGGAGGACGAUGCGGU UUGAAGGGAGAUGGCUCCGCAGACGACUCGCCCGA 2067. .H32 3657_H04
CGACAAGTCTGTACCCTCCC GGGAGGACGAUGCGGCGACAAGUCUGUACCCUCCCCAGACGACUCGCCCGA 2068. .H32
TTTGAAAGGCGATGGGTCCG GGGAGGACGAUGCGGU UUGAAAGGCGAUGGGUCCGCAGACGACUCGCCCGA 2069. .H32 454Z_H04
TTTGTAG G GTGTTG GGT CCG GGGAGGACGAUGCGGUUUGUAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 2070. _H32 2688 H04
TTTGACGGGTGGTGGGTCCG G6GAGGACGAUGCGGUUUGACGGGUGGUGG6UCCGCAGACGACUCGCCC6A 2071 H32
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
AGAGTTAATCTGTCTGTGGC GGGAGGACGAUGCGGAGAGUUAAUCUGUCUGUGGCCAGACGACUCGCCCGA 2072_ _H32
TATTGGAAGTGTAGGGACCG GGGAGGACGAUGCG6UAUUGGAAGUGUAGGGACCGCAGACGACUCGCCCGA 2073. H32
ATTGGAAGGGAGGTACTG GGGAGGACGAUGC6GAUUGGAAGGGAGGUACUGCAGACGACUCGCCCGA 2074. .H32
CCGTCCATGTTACACCTCTC GGGAGGACGAUGCGGCCGUCCAUGLiUACACCUCUCCAGACGACUCGCCCGA 2075. H32
TTG6GGGTATGGTGGTACCG GGGAGGACGAUGCGGU UGGGGGUAUGGUGGUACCGCAGACGACUCGCCCGA 2076. _H32
CACACGCGTTATGTCTACCC GGGAGGACGAUGCGGCACACGCGUUAUGUCUACCCCAGACGACUCGCCCGA 2077_ _H32
CCG TAATAG TCCCTG ATCCC GGGAGGACGAUGCGGCCGUAAUAGUCCCUGAUCCCCAGACGACUCGCCCGA 2078. .H32
ATCATCAGGCTGTATACCGC GGGAGGACGAUGCGGAUCAUCAGGCUGUAUACCGCCA6ACGACUCGCCCGA 2079. _H32
TTGTCTACCTTCTACTG CCG GGGAGGACGAUGCGGUUG UCUACCUUCUACUGCCGCAGACGACUCGCCCGA 2080, H32
TCTGAAGGGAGATGGGTCCG GGGAGGACGAUGCGGUCUGAAGGGAGAUGGGUCCGCAGACGACUCGCCCGA 2081_ H32
ACACTACAAAATGGTCG CTG GGGAGGACGAUGCGGACACUACAAAAUGGUCGCUGCAGACGACUCGCCCGA 2082. H32
GACGGAGGTGTCGTCGTG TG G6GAGGACGAUGCGGGACG6AGGUGUCGUCGUG UGCAGACGACUCGCCCGA 2083. _H32
ATTGAAGGGTGGTGGGTCCG GGGA6GACGAUGCGGAUUGAAGGG UGGUGGGUCCGCAGACGACUCGCCCGA 2084, H32
GATT6GAGGTGTA6GGACCG GGGAGGACGAUGCGGGAUUGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 2085.. H32
ACCCCAATGTAGTCTGTGGC GGGAGGACGAUGCGGACCCCAAUG SJAGUCUGUGGCCAGACGACUCGCCCGA 2086. .H32
AG TCGTCTTTCG AG CCTGAC GGGAGGACGAUGCGGAGUC6UCUUUCGAGCCUGACCA6ACGACUCGCCCGA 2087. _H32
ATTGTGAATGGAGCCGGCTG GGGAGGACGAUGCGGAUUGUGAAUGGAGCCGGCUGCAGACGACUCGCCCG 2088. H32
CAGTCCTGTCGTCGTGTAGC GGGAGGACGAUGCGGCAGUCCUGUCGUCGU6UAGCCAGACGACUCGCCCGA 2089. H32
TTTGAAGGGGGATGAGTCCG GGGAGGACGAUGCGGU UUGAAGGGGGAUGAGUCCGCAGACGACUCGCCCGA 2090^ -H32 5086 H04
GAT6 AG TCCAACCCCGTGTG GGGAGGACGAUGCGGGAUGAGUCCAACCCCGUGUGCAGACGACUCGCCCGA 2091_ H32
TT7GATGGGGGGTGGGACCG GGGAGGACGAUGCGGUUUGAUGGGGGGUGGGACCGCAGACGACUCGCCCGA 2092. .H32
GTG CTCTTGTCTCTCG TGTG GGGAGGACGAUGCGGGUGCUCUUGUCUCUCGUGUGCAGACGACUCGCCCGA 2093. H32
TCGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGUCGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2094. _H32 4460 H04
ATTTGCGTTCTCTCGAGCCG GGGAGGACGAUGCGGAUUUGCGUUCUCUCGAGCCGCAGACGACUCGCCCGA 2095. _H32
ACACTGGAGAACCTGTGCCC GGGAGGACGAUGCGGACACUGGAGAACCUGUGCCCCAGACGACUCGCCCGA 2096_ H32
TTCGATTGGTCCCCAGTCCG GGGAGGACGAUGCGGUUCGAUUGGUCCCCAGUCCGCAGACGACUCGCCCGA 2097. H32
TTCGTTTGTCCCG6CTGCGT GGGAGGACGAUGCGGUUCG UUUGUCCCGGCUGCGUCAGACGACUCGCCCGA 2098. _H32
CAAAGCGCCTCCAACAGTAC GGGA6GACGAUGCGGCAAAGCGCCUCCAACAGUACCAGACGACUCGCCCGA 2099 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATCCAAGGGTAAACCAGGTG G GG AGG ACG AUGCGG A UCCAAGG G UAAACCAGG UGCAGACG ACU CGCCCGA 2100_ .H32
TAACATG CGTACCCCCTCCC GGGAGGACGAU6CGGUAACAUGCGUACCCCCUCCCCAGACGACUCGCCCGA 2101. .H32
TTGGGTGGAGGGCGGTACCG GGGAGGACG AUGCGG U UGGGUGGAGGGCGG UACCGCAG ACG ACUCGCCCGA 2102, _H32
TCCGAATAATATTGCGCCGC GGGAGGACGAUGCGGUCCGAAUAAUAUUGCGCCGCCAGACGACUCGCCC6A 2103. „H32
TCCTGTGTCTTTGCGTCCC 6GGAGGACGAUGCGGUCCUGUGUCUUUGCGUCCCCAGACGACUCGCCCGA 210 _H32 3791 H04
CTGGGTGTAGGGCGGTACCG GGGAGGACGAUGCGGCUGGGUGUAGGGCGG UACCGCAGACG ACUCGCCCGA 2105_ _H32
TAGGAAACCTCCTTGCGTGC GGGAGGACGAUGCGGUAGGAAACCUCCUUGCGUGCCAGACGACUCGCCCGA 2106_ H32
ATAGGGAGAAGGGTGAGCCG G6G AGG ACGAUGCGGAUAGGGAGAAGG UGAGCCGCAG ACG ACUCGCCCGA 2107. H32
GTGG CCTGTTCCTCTGTCCG GGGAGGACGAUGCGGG UGGCCUGUUCCUCUGUCCGCAGACG ACUCGCCCGA 2108_ H32
CAAACGGTTAAACTAG CGTG GGGAGGACGAUGCGGCAAACGGU UAAACUAGCGUGCAGACG ACUCGCCCGA 2109.. .H32
CAATAGATAAGCCCGGTGCC GGGAGGACGAUGCGGCAAUAGAUAAGCCCGG UGCCCAGACGACUCGCCCGA 2110. _H32
TCCTTGCTCTGCTGTATGCC GGGAGGACGAUGCGGUCCUU6CUCUGCUGUAUGCCCAGACG ACUCGCCCGA 2111. H32
ACAGTCCTCTTGGTTG CCCC GGGAGGACGAUGCGGACA6UCCUCUUGGUUGCCCCCA6 ACG ACUCGCCCGA 2112_ .H32
TGTAGGAGGAGGTGGGACCG GGGAGGACG AUGCGGUGU AGG AGG AGGUGGGACCGCAGACGACUCGCCCGA 2113_ .H32
TACCTCCTAGTGGTG GTCCC GGGAGGACG AUGCGGUACCUCCUAGUG6UG6UCCCCAGACGACUCGCCC6A 2114 _H32
ATCAACCTTCCTGACGTGTG GGGAGGACGAUGCGGAUCAACCUUCCUGACGUGUGCAGACGACUCGCCCGA 2115_ .H32
TCAACTCACAAACCTGTCG C GG6AGGACGAUGCGGUCAACUCACAAACCUGUCGCCAGACGACUCGCCCGA 2116_ .H32
ATTGGAGAAGGGAGGAACTG GGGAGGACGAUGCGGAU UGGAGAAGGGAGGAACUGCA6AC6ACUCGCCCGA 2117_ .H32
TTGGGAGCAGGGTGGTACCG GGGAGGACGAUGCGGU UGGGAGCAGGGUGGUACCGCAGACGACUCGCCCGA 2118_ ,K32
ATTGGTGGAGGGTGGGACTG G6GAGGACGAUGCGGAUUGGUGGAGGGUGGGACUGCAGACGACUCGCCCGA 2119. _H32
TTGGG AGTATGGTGGTACCG GGGAGGACGAUGCGGUUGGGAGUAUGGUGGUACCGCAGACG ACUCGCCCGA 2120_ H32
TTTGAAGGGAGATGGGTTCG GGGAGGACGAUGCGGUUUGAAGGGAGAUGGGUUCGCAGACGACUCGCCCGA 2121_ H 32
C ACC G TCG CTGTTTACG CCC GGGAGGACGAUGCGGCACCGUCGCUGUUUACGCCCCAGACGACUCGCCCGA 2122_ .H32
CTTGAAGGGTGTTGGGTCCG GGGAGGACGAUGCGGCU UGAAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 2123. .H32 3715..H04
TTTGAAGGGTGAGGGGGCCG GGGAGGACG AUGCGG UUUGAAGGGUGAGGGGGCCGCAGACGACUCGCCCGA 2124. H32 4494 H04
ACGAACAATGGGGGTTGCCC GGGAGGACGAUGCGGACGAA.CAAUGGGGCU UGCCCCAGACGACUCGCCCGA 2125. _H32
TTTGGAGAAGGATGGGACCG GGGAGGACGAUGCGGUUUGGAGAAGGAUGGGACCGCAG ACG ACUCGCCCGA 2126. H32
TTTTGAAGGGAGATGGGTCCG GGGAGGACG AUGCGGUUUUGAAGGGAGAUGGGUCCGCAGACGACUCGCCC6A 2127 H32 4961 H04
tt in H4
HIS read variable region RNA sequences U in H3 selection selection Selected sequenc
ATGAAGGGTGATCCGTCCC GGGAGGACGAUGCGGAUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2128_ ,H32 4488. H04
TTTG A AG G GCG ATCG GTCCG GGGAGGACGAUGCGGUUU6AAGGGCGAUCGGUCCGCAGACGACUCGCCCGA 212 _ _H32
TTTGAAGGGAGATGGGTCGG GGGAGGACGAUGCGGU UUGAAGGGAGAUGGGUCGGCAGACGACUCGCCCGA 2130. _H32 4424. .H04
CTTGTCGTTTTGTTCGTCCC GGGAGGACGAb'GCGGCUUGUCGUU U UGUUCGLCCCCAGACGACUCGCCCGA 2131. _H32
ATTTGGAGAAGGGAGGTG GGGAGGACGAUGCGGAULUGGAGAAGGGAGG UGCAGACGACUCGCCCGA 2132. . H32
ATTCCCTCCGTTCTCGTTAC GGGAGGACGAUGCGGAUUCCCUCCGUUCUCGUUACCAGACGACUCGCCCGA 2133. _H32
TTTAAAGGGTGGTGGGTCCG GGGAGGACGAUGCGGU UUAAAGGGUGGUGGGUCCGCAGACGACUCGCCCGA 2134. .H32 5061. JH04
TTTGAAGGGTGATGGTTCCC GGGAGGACGAUGCGGU UUGAAGGG UGAUGG UUCCCCAGACGACUCGCCCGA 2135. .H32 4355. „H04
GCTTCGTTCTGACCTCCC GGGAGGACGAUGCGGGCUUCGU UCUGACCUCCCCAGACGACUCGCCCGA 2136. .H32
TTG G A AG G AG G G TG GTACCG GGGAGGACGAUGCGGUUGGAAGGAGGGUGGUACCGCAGACGACUCGCCCGA 2137, _H32
TTTGAAGTGCGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGUGCGAUGGG UCCGCAGACGACUCGCCCGA 2138. _H32 3118. .H04
TTG G AAGG AGGATGGGACCG GGGAGGACGAUGCGGUUGGAAGGAGGAUGGGACCGCAGACGACUCGCCCGA 2139. H32
TAAACCGTCCAT CTGTCCC GGGAGGACGAUGCGGUAAACCGUCCAUCCUGUCCCCAGACGACUCGCCCGA 2140. _H32
TTCTCCATCGTTGTGCGCCA GGGAGGACGAUGCGGUUCUCCAUCGUUGUGCGCCACAGACGACUCGCCCGA 2141. _H32
TTTGGAGGGGGA.TGGTACCG GGGAGGACGAUGCGGUUUGGAGGGGGAUGG UACCGCAGACGACUCGCCCGA 2142. . H32
CAAGTCGCTGTTTTCCTGCC GGGAGGACGAUGCGGCAAGUCGCUGUUUUCCUGCCCAGACGACUCGCCCGA 2143. „H32
TTGG G AG 6 AG G G TG G GTCCG GGGAGGACGAUGCGGU UGGGAGGAGGGUGGGUCCGCAGACGACUCGCCCGA 2144. _H32
CTTGAAGTGTGATGGGTCCG GGGAGGACGAUGCGGCUUGAAC UGUGAUGGGUCCGCAGACGACUCGCCCGA 2145. ,H32
GTTGAAGTGTGATGGGTCCG GGGAGGACGAUGCGGGUUGAAGUGUGAUGGGUCCGCAGACGACUCGCCCGA 2146. .H32
TTGTGTGTAG 6G GGGT ACCG GGGAGGACGAUGCGGUUGU6UGUAG6GG6GUACCGCAGACGACUCGCCCGA 2147. H3
TTTGAAGGGTGAGGGGTG GGGAGGAC6AUGCGGUUUGAAGGGUGAGGG6UGCAGACGACUCGCCCGA 2148. .H32
ATTGGGAGAAGGGTGAACCG GGGAGGACGAUGCGGAUUGGGAGAAGGGUGAACCGCAGACGACUCGCCCGA 2149. _H32
AG ATCG ATG GGTTCTCG TG CC GGGAGGACGAUGCGGAGAUCGAUGGGUUCUCGUGCCCAGACGACUCGCCCGA 2150. .H32
CAAGTCGTCCTTGGTGTGTG GGGAGGACGAUGCGGCAAGUCGUCCUUGGUGUGUGCAGACGACUCGCCCGA 2151. .H32
TATCTAG G CG CCTATG ACTG GGGAGGACGAUGCGGUAUCUAGGCGCCUAUGACUGCAGACGACUCGCCCGA 2152. _H32
TGTAGTCGGTGCATTGTGGC GGGAGGACGAUGCGGUG UAGUCGGUGCAU UGUGGCCAGACGACUCGCCCGA 2153. .H32
CCnGTCGTCTQTTCGTCCC GGGAGGACGAUGCGGCCUUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 215 „ .H32
C AA A A AAG TAAA~GTC ACTG GGGAGGACGAUGCGGCAAAAAAGljAAAUGUCACUGCAGACGACUCGCCCGA 2155 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TG AGTCTAAGT ATGTG 6 CTG GGGAGGACGAUGCGGUGAGUCUAAGUAUGUGGCUGCAGACGACUCGCCCGA 2156_ .H32
TCCTTGTCGTCTT'GCGTCCG GGGAGGACGAUGCGGUCCUUG UCGUCUUGCGUCCGCAGACGACUCGCCCGA 2157. .H32
CTATTGGTGGAGGGTGGTG GGGAGGACGAUGCG6CUAU UGGUGGAGGGUG6UGCAGACGACUCGCCCGA 2158. .H32
TAGTAGTTAATCCTGTGCCC GGGAGGACGAUGCGGUAGUAG UUAAUCCUGUGCCCCA6ACGACUCGCCCGA 21S9. _H32
GCAACTAAGCCTCCGTGAC GGGAGGACGAUGCGGGCAACUAAGCCUCCGUGACCAGACGACUCGCCCGA 2160. _H32
GTTTCAAGGTAACGTCTGGC GGGAGGACGAUGCGGG UUUCAAGGUAACGUCUGGCCAGACGACUCGCCCGA 2161. H32
CATCG G TG CTCCGTCGTATA GGGAGGACGAUGCGGCAUCGGUGCUCCGUCGUAUACAGACGACUCGCCCGA 2162. H32
TTGGGTGGGGGGTGGTACCG GGGAGGACGAUGCGGU UGGGUGGG6GGUGGUACCGCAGACGACUCGCCCGA 2163 _ .H32
GCGTTCACCGTCGGTCTGTG GGGAGGACGAUGCGGGCGU UCACCG UCGGUCUGUGCAGACGACUCGCCCGA 2164. .H32
TTAGACATGACAGGCCGCGC GGGAGGACGAUGCGGUUAGACAUGACAGGCCGCGCCAGACGACUCGCCCGA 165. _H32
GATTCGTGCTGG CATATGTC GGGAGGACGAUGCGGGAUUCGUGCUGGCAUAUG UCCAGACGACUCGCCCGA 2166. _H32
AAAAGTCCTTGAGTGTGCCC G6GAGGACGAUGCGGAAAAGUCCUUGAGUGUGCCCCAGACGACUCGCCCGA 2167. .H32
TGTTCCCCTGGATCGTTGGC GGGAGGACGAUGCGGUGUUCCCCUGGAUCGUUGGCCAGACGAGUCGGCCGA 2168. H32
GATTCGTCCCCGATCGTTGG GGGAGGACGAUGCGGGAU UCGUCCCCGAUCGU UGGCAGACGACUCGCCCGA 2169. .H32
TACCGTCGTTTTTGGCTTGGC GGGAGGACGAUGCGGUACCGUCGUUUUUGGCUUGGCCAGACGACUCGCCCGA 2170. JH32
CCCTGTGAAAATGTGCTGTG GGGAGGACGAUGCGGCCCUGUGAAAAUGUGCUGUGCAGACGACUCGCCCGA 2171. _H32
TT6GCTTGTCGTTGTCCC GGGAGGAC6AUGCGGUUGGCUUGUCGUUGUCCCCAGACGACUCGCCCGA 2172_ H32
TCCAGCTCCTTTCACCTCCC GGGAGGACGAUGCGGUCCAGCUCCUU UCACCUCCCCAGACGACUCGCCCGA 2173. H32
CGTGCTCATACGGTCTGGCC GGGAGGACGAUGCGGCGUGCUCAUACGGUCUGGCCCAGACGACUCGCCCGA 2174. „H32
AAATCAGTGTGCCGCGACAC GGGAGGACGAUGCGGAAAUCAGUGUGCCGCGACACCAGACGACUC6CCCGA 2175. H32
ATTCGG TAAA TTACGT CTG GG6AGGACGAUGCGGAUUCGGUAAAGUUACGUCUGCAGACGACUCGCCCGA 2176. ^H32
GCCAAATGTCCTCTGCGCGC GGGAGGACGAUGCGGGCCAAAUGUCCUCUGCGCGCCAGACGACUCGCCCGA 2177. .H32
AATCGTTACTTATCG CTGGT GGGAGGACGAUGCGGAAUCGUUACUUAUCGCb'GGUCAGACGACUCGCCCGA 2178. .H32
AATGATATCCTCAT7ACGTG GGGAGGACGAUGCGGAAUGAUAUCC'JCAUUACG UGCAGACGACUCGCCCGA 2179. . H32
TCTG GCAACATGCTCCACCC GGGAGGACGAUGCGGUCUGGCAACAUGCUCCACCCCAGACGACUCGCCCGA 2180. .H32
AGGACGGTGTTCGGTCAGTA GGGAGGACGAU6CGGAGGACGGUG UUCGGUCAGIJACAGACGACUCGCCC6A 2181. _H32
GA6CGATGATATGACATCCG GGGAGGACGAUGCGGGAGCGAUGAUAUGACAUCCGCAGACGACUCGCCCGA 2182. _H32
TTGGGTGGAGGGTGTTACCG GGGAGGACGAUGCGGU UGGGUGGAGGG UGUUACCGCAGACGACUCGCCCGA 2183 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATACGATCCTCCAGGTGCCC GGGAGGACGAUGCGGAUACGAUCCU.CCAGGUGCCCCAGACGACUCGCCCGA 2184 . H32
CATCCGTCCGGCGTACATGC GGGAGGACGAUGCGGCAUCCGUCCGGCG UACAUGCCAGACGACUCGCCCGA 2185_ .H32
TCACCCTTGGCTTTCCACCG GGGAGGACGAUGCGGUCACCCUUGGCUUUCCACGGCAGACGACUCGCCCGA 2186_ .H32
CGTTGCGATCTGTCCGTGTG GGGAGGACGAUGCGGCG UUGCGAUCUGUCCGUGUGCAGACGACUCGCCCGA 2187. H32
GTGCGTCATGTCTGTCTGCC GGGAGGACGAUGCGGGUGCGUCAUGUCUGUCUGCCCAGACGACUCGCCCGA 2188, H32
TTGGGTGCAGGGAGGTACCG GGGAGGACGAUGCGGUUGGGUGCAGGGAGG UACCGCAGACGACUCGCCCGA 2189, H32
CTAAGAGGAGGAGATGGGAG GGGAGGACGAUGCGGCUAAGAGGAGGAGAUGGGAGCAGACGACUCGCCCGA 2190. H32
CATCGTGCACTCCGCGTGTC GGGAGGACGAUGCGGCAUCG UGCACUCCGCGUG UCCAGACGACUCGCCCGA 2191 . H32
TTAGCTGCGCACAG CATGCC GGGAGGACGAUGCGGUUAGCUGCGCACAGCAUGCCCAGACGACUCGCCCGA 2192_ H32
TCTTCCATTAGCGCAGTGCC GGGAGGACGAUGCGGUCUUCCAUUAGCGCAGUGCCCAGACGACUCGCCCGA 2193_ H32
AAC AAG CCTCTCCG TTGCC C GGGAGGACGAUGCGGAACAAGCCUCUCCGUUGCCCCAGACGACUCGCCCGA 2194_ H32
TAAGCACG ATAATCCCCCCC GGGAGGACGAUGCGGUAAGCACGAUAAUCCCCCCCCAGACGACUCGCCCGA 2195_ H32
GATTGGGTCGTCCCACTGTG GGGAGGACGAUGCGGGA'UUGGGUCGUCCCACUGUGCAGACGACUCGCCCGA 2196. H32
CTTG CGTTATG GCGTTGTGC GGGAGGACGAUGCGGCUUGCGUUAUGGCGUUGUGCCAGACGACUCGCCCGA 2197. H32
TTGAGTGTAGGGTGTTACCG GGGAGGACGAUGCGGU UGAGUGUAGGGUGU UACCGCAGACGACUCGCCCGA 2198_ H32
AACCATGGCTTCCCCGTGTC GGGAGGACGAUGCGGAACCAUGGCUUCCCCGUGUCCAGACGACUCGCCCGA 2199. H32
GTTTGGTAAGCGCCTCTGTG GGGAGGACGAUGCGGGUUUGG UAAGCGCCUCUGUGCAGACGACUCGCCCGA 2200_ H32
ATAACTGGGTAC7 CT6GCCG GGGAGGACGAUGCGGAUAACUGGGUACUCUGGCCGCAGACGACUCGCCCGA 2201. .H32
AGTCG CTG ATGTTG TCG CTG GGGAGGACGAUGCGGAGUC.GCUGAU6UU6UCGCUGCAGACGACUCGCCCGA 2202_ .H32
TCTGTCCGGGTCTGTCGCTA GGGAGGACGAUGCGGUCUG UCCGGG UCUGUCGCUACAGACGACUCGCCCGA 2203_ H32
ACGGCIATTAAGCTACTCGC GGGAGGACGAUGCGGACGGCUAUUAAGCUACUCGCCAGACGACUCGCCCGA 2204_ H32
GTAGTGCTCTGGTACGCGGC GGGAGGACGAUGCGGGUAGUGCUCUGGUACGCGGCCAGACGACUCGCCCGA 2205_ H32
AAAGAACACCTCATCTTGTC GGGAGGACGAUGCGGAAAGAACACCUCAUCUUGUCCAGACGACUCGCCCGA 2206_ H32
TATTCGAGCACATACGTGTG GGGAGGACGAUGCGGUAUUCGAGCACAUACGUG UGCAGACGACUCGCCCGA 2207., H32
ACCTCCAATAATATGTGCGC GGGAGGACGAUGCGGACCUCCAAUAAUAUGU6CGCCAGACGACUCGCCCGA 2208. H32
GTGACGGTGCCTTG AGTGTG G6GAGGACGAUGCG6GU6ACGGUGCCUUGAGUGUGCAGACGACUCGCCCGA 2209. H 32
TCTAAACTCTCCTG CACCTG GGGAGGACGAUGCGGUCUAAACUCUCCUGCACCUGCAGACGACUCGCCCGA 2210_ H32
ACCAGCGAGTATGTGCGCAC GGGAGGACGAUGCGGACGAGCGAGUAUGUGCGCACCAGACGACUCGCCCGA 2211 H32
# in H4
HTS read variable region R A sequences # in H3 selection selection Selected sequenc
TATCCGGAACGTGCTGTCGC G6GAGGACGAUGCGGUAUCCGGAACGUGCUGUCGCCAGACGACUCGCCCGA 2212. „H32
TCTAAAGTG CACTGTGTCCC GGGAGGACGAUGCGGUCUAAAGUGCACUG UGUCCCCAGACGACUCGCCCGA 2213. ,H32
TCTTGTCGTCTT GCGTCCC GGGAGGACGAUGCGGUCUUGUCGUCUUGCGUCCCCAGACGACUCGCCCGA 2204. .H32
TACGAAAGAACAATCTTGTG GGGAGGACGAUGCGGUACGAAAGAACAAUCUUG JGCAGACGACUCGCCCGA 2215. _H32
GAATAGTGTGTGTGGTTGGC GGGAGGACGAUGCGGGAAUAG UGUGUGUGGUUGGCCAGACGACUCGCCCGA 2216. _H32
CGTTCACCCTGTCGTTTGGC GGGAGGACGAUGCGGCGUUCACCCUGUCGUUUGGCCAGACGACUCGCCCGA 2217. _H32
GTCCTGTCGTCTGmrrccc GGGAGGACGAUGCGGGUCCUGUCGUCUGUUCUUCCCCAGACGACUCGCCCGA 2218. .H32 4766 H04
ATGTCTATG G CTACACGG GC GGGAGGACGAUGCCGAUGUCUAUGGCUACACGGGCCAGACGACUCGCCCGA 2219. ,H32
TACCGAGAGTTCTTCACCGC G GGAGGACGAUGCGGUACCG AGAG U UCUUCACCGCCAGACGACUCGCCCGA 2220. .H32
CCATGTTAACAGTCACTCCC GGGAGGACGAUGCGGCCAUG UUAACAGUCACUCCCCAGACGACUCGCCCGA 2221. _H32
TCACAGTAGCATG CCGTCCC GGGAGGACGAUGCGGUCACAGUAGCAUGCCGUCCCCAGACGACUCGCCCGA 2222. _H32
CCGTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGCCGUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 2223. _H32
TCCATTCCCGTTAACGGCCG GGGAGGACGAUGCGGUCCAU UCCCGUUAACGGCCGCAGACGACUCGCCCGA 2224. ,H32
TACCTCGGCTTCTGTGTGCA GGGAGGACGAUGCGGUACCUCGGCUUCUG UGUGCACAGACGACUCGCCCGA 2225. .H32
TG TGGTTG TCTGG TG ATGTG GGGAGGACGAUGCGGUGUGGUUGUCUGGUGAUGUGCAGACGACUCGCCCGA 2226. .H32
ACGGCGCGTTGTCTCGTCAC GGGAGGACGAUGCGGACGGCGCGUUGUCUCGUCACCAGACGACUCGCCCGA 2227. _H32
GIG ATTGTTG AG G TCTG G CC GGGAGGACGAUGCGGG UGAUUGUUGAGGUCUGGCCCAGACGACUCGCCCGA 2228. .H32
TCATTTATTCCGTCTGTGCC GGGAGGACGAUGCGGUCAU UUAU UCCG UCUGUGCCCAGACGACUCGCCCGA 2229. „H32
GTACGTGAACTCGTTGTGGC GGGAGGACGAUGCGGG UACGUGAACUCGUUGUGGCCAGACGACUCGCCCGA 2230. ,H32
CATAAGTGAGTGTGAGCCCC GGGAGGACGAUGCGGCAUAAGUGAGUGUGAGCCCCCAGACGACUCGCCCGA 2231. _H32
TGTAATTAG TTTGTACTGTG GGGAGGACGAUGCGGUGUAAUUAGU UUGUACUG UGCAGACGACUCGCCCGA 2232. _H32
TTTCACGTCATGCCTGTGTC GGGAGGACGAUGCGGUUUCACGUCAUGCCUGUGUCCAGACGACUCGCCCGA 2233. _H32
TTAGCTTGACGTGCCGCGTG GGGAGGACGAUGCGGUUAGCLi UGACGUGCCGCGUGCAGACGACUCGCCCGA 2234. .H32
TCCTTTCGAGTATTCGTCTG GGGAGGACGAUGCGGUCCU UUCGAGUAUUCGUCUGCAGACGACUCGCCCGA 2235. .H32
TTTGATGGGTGATGGGTCCC GGGAGGACGAUGCGGU UUGAUGGGUGAUGGGUCCCCAGACGACUCGCCCGA 2236. _H32 3530 H04
CCTGCAACATTGTGCCATAC GGGAGGACGAUGCGGCCUGCAACAUUGUGCCAUACCAGACGACUCGCCCGA 2237. „H32
ATACATTGTGACTACCGTGC GGGAGGACGAUGCGGAUACAUUGUGACUAf.C UGCCAGACGACUCGCCCGA 2238. .H3
AC CTTA ACA ATTCTG CGG CC GGGAGGACGAU6CGGACCUUAACAAUUCUGCGGCCCAGACGACUCGCCCGA 2239 H32
# in H4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc
TTTGAAGGGTGATAGGTCCC GGGAGGACGAUGCGG U UUGAAGGGUGAUAGGUCCCCAGACGACUCGCCCGA 2240_H32
AAAC ACCTCCTATG CGTG AC GGGAGGACGAUGCGGAAACACCUCCUAUGCG UGACCAGACGACUCGCCCGA 2241_H32
TCGTCGCGTAACTCTGCCTG GGGAGGACGAUGCGGUCGUCGCGUAACUCUGCCUGCAGACGACUCGCCCGA 2242_H32
CCCAGTAGTCTGCACCTGTA GGGAGGACGAUGCGGCCCAG UAG UCUGCACCUGUACAGACGACUCGCCCGA 2243._H32
AC ACTCCATTA AG CCTCCTG GGGAGGACGAUGCGGACACUCCAUUAAGCCUCCUGCAGACGACUCGCCCGA 2244_H32
TCTGCGTATCGTTTGTGCCC GGGAGGACGAUGCGGUCUGCGUAUCGU UUGUGCCCCAGACGACUCGCCCGA 2245_H32
CGATGGTTCTCTTCAGTGTG GGGAGGACGAUGCGGCGAUGGUUCUCUUCAGUGUGCAGACGACUCGCCCGA 2246_ H32
ATAGCCGATCTTTGCCACGT GGGAGGACGAUGCGGAUAGCCGAUCUUUGCCACGUCAGACGACUCGCCCGA 2247_H32
ATTGGTGAAGGGAGGTAACG GGGAGGACGAUGCGGAUUGGUGAAGGGAGGUAACGCAGACGACUCGCCCGA 2248_H32
CGATTCACCTG GCTCCGCAC GGGAGGACGAUGCGGCGAU UCACCUGGCUCCGCACCAGACGACUCGCCCGA 2249_ H32
TTGATCATCCTCGCTGCGTG GGGAGGACGAUGCGGUUGAUCAUCCUCGCUGCGUGCAGACGACUCGCCCGA 225D_H32
CTCTAGTTCCTCCTGTGTGC GGGAGGACGAUGCGGCUCUAGUUCCUCCUGUGUGCCAGACGACUCGCCCGA 2251_H32
ACCGTGTCTGTGGTGTGGTG GGGAGGACGAUGCGGACCGUGUCUG UGGUGUGGUGCAGACGACUCGCCCGA 2252_H32
ATAAGTGGTTGTAGTCGTGC GGGAGGACGAUGCGGAUA^GUGGU UGUAGUCGUGCCAGACGACUCGCCCGA 2253_H32
TATC A ATTGG A ATG GTG C G C GGGAGGACGAUGCGGUAUCAAU UGGAAUGGUGCGCCAGACGACUCGCCCGA 2254_ H32
T GACTCGTCTATTTCGTCCC GGGAGGACGAUGCGGUGACUCG UCUAUUUCGUCCCCAGACGACUCGCCCGA 2255„H32
TTCGTGGGCTATGACGCGCC GGGAGGACGAUGCGGUUCGUGGGCUAUGACGCGCCCAGACGAC'JCGCCCGA 2256_H32
GAACATGTAACAAGGTGCTG GGGAGGACGAUGCGGGAACAUGUAACAAGGUGCUGCAGACGACUCGCCCGA 2257_H32
TTCG TGTATCTG GG TG CTAC GGGAGGACGAUGCGGUUCG UGUAUCUGGGUGCUACCAGACGACUCGCCCGA 2258_H32
CGTTTCCTCTGGTTCGTCCA GGGAGGACGAUGCGGCGUUUCCUCUGGU UCGUCCACAGACGACUCGCCCGA 2259_H32
GC6AAGGGTGATGGGTCCG GGGAGGACGAUGCGGGC6AAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2260_H32 4659 HQ4
TGGTCTACGCGTATCGTGTG GGGAGGACGAUGCGGUGGUCUACGCGUAUCGUGUGCAGACGACUCGCCCGA 2261_H32
TTTGAAGGGCGATGGGTCGG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGUCGGCAGACGACUCGCCCGA 2262_H32 4674 H04
TGACTCGTCCGTTTCGTCCC GGGAGGACGAUGCGGUGACUCGUCCGUUUCGUCCCCAGACGACUCGCCCGA 2263_H32
AACTCCTTCTCTCGGCTCGC GGGAGGACGAUGCGGAACUCCUUCUCUCGGCUCGCCAGACGACUCGCCCGA 2264_K32
TTTGAAGTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGUGAUGGGUCCGCAGACGACUCGCCCGA 2265_H32
TATTGGAGGTGTAGGGACCT GGGAGGACGAUGCGGUAUUGGAGG UGUAGGGACCUCAGACGACUCGCCCGA 2266_H32
TTTTTGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUUUGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2267 H32
# ίη H4
NTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TATTGTAGGTGTAGGGACCG GGGAGGACGAUGCGGUAU UGUAG6UGUAGGGACCGCAGACGACUCGCCCGA 2268__H32
TTTG AAG G GG G ACG GGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGACGGGUCCGCAGACGACUCGCCCGA 2269„H32 4732_ .H04
TGAGTCCCTAGGTATGCCCC GGGAGGAC6AUGCGGUGA6UCCCUAGGUAUGCCCCCAGACGACUCGCCCGA 2270„H32
CTTG A AG G GTG ATG AGTCCG GGGAGGACGAUGCGGCUUGAAGGGUGAUGAGUCCGCAGACGACUCGCCCGA 2271_H32 5174.. .H04
TTG GGT6TTG G G TGTTACCG GGGAGGACGAUGCGGU UGGGUGUUGGGUGUUACCGCAGACGACUCGCCCGA 2272_H32
TTGGTGTAGGGTGGTACTG GGGAGGACGAUGCGGU UGGUGU'AGGGUGGUACUGCAGACGACUCGCCCGA 2273_H32
AGAGTCTATGAGTGTGACCC GGGAGGACGAUGCGGAGAG UCUAUGAGUGUGACCCCAGACGACUCGCCCGA 2274J 32
TTTGAAGGCGG ATG GGTCCG GGGAGGACGAUGCGGU UUGAAGGCGGAUGGGUCCGCAGACGACUCGCCCGA 2275_H32 4944_ .H04
TTATG TTCAAGTG ACGTG G C GGGAGGACGAUGCGGU UAUGUUCAAGUGACGUGGCCAGACGACUCGCCCGA 2276_H32
GG CGGTA AAC ACG CTGTGGC GGGAGGACGAUGCGGGGCGGUAAACACGCUGUGGCCAGACGACUCGCCCGA 2277_H32
TGCGTCATCTGGTTCATGGC GGGAGGACGAUGCGGUGCG UCAUCUGG UUCAUGGCCAGACGACUCGCCCGA 2278_H32
GTCGAGATCGACTCGTTTGC GGGAGGACGAUGCGGGUCGAGAUCGACUCGUUUGCCAGACGACUCGCCCGA 2279_H32
TTGGGTGTATGGCGGTACCG GGGAGGACGAUGCGGUUGGGUGUAUGGCGG UACCGCA6ACGACUCGCCCGA 2280_H32
CCATTC6AGCTGGCAGTGCC GGGAGGACGAUGCGGCCAUUCGAGCUGGCAGUGCCCAGACGACUCGCCCGA 2281_H32
TTTGAAGGATGATGTCCG GGGAGGACGAUGCGGUUUGAAGGAUGAUGUCCGCAGACGACUCGCCCGA 2282„H32
TGGCCGTAAAAGTGTGGCCC GGGAGGACGAUGCGGUGGCCGUAAAAGUGUGGCCCCAGACGACUCGCCCGA 2283_H32
TTCACTGTAGTATCCACTAC GGGAGGACGAUGCGGUUCACUGUAGUAUCCACUACCAGACGACUCGCCCGA 228.4_H32
TTGGGTGAAGGGTGGCACCG G6GAGGACGAUGCGGUUGGGUGAAGGGUGGCACCGCAGACGACUC6CCCGA 2285_H32
TATCGTATTGTTTATCCCAC GGGAGGACGAUGCGGUAUCGUAUUGUUUAUCCCACCAGACGACUCGCCCGA 2286_H32
GCTTTC6TTCAGCCCTCCC GG6AGGACGAUGCGGGCUUUCG UUCAGCCCUCCCCAGACGACUCGCCCGA 2287_H32
CA AG TC ATTA GG G CTG TTG C GGGAGGACGAUGCGGCAAGUCAU UAGGGCUGUUGCCAGACGACUCGCCCGA 2288„H32
TGTGTTGTCTGTCTGGGGTC GGGAGGACGAUGCGGUGUG UU6UCUGUCUGGGGUCCAGACGACUCGCCCGA 2289_H32
TCCTTGTTGTCTTGCGTCCC GGGAGGACGAUGCGGUCCUUGU UGUCUUGCGUCCCCAGACGACUCGCCCGA 2290_H32 2170._ _H04
TGT A A AG TCG TCCC6TG CCC GGGAGGACGAUGCGGUGUAAAGUCGUCCCG UGCCCCAGACGACUCGCCCGA 2291_H32
TTGGGAGGAGGATGGGTCCG GGGAGGACGAUGCGGUUGGGAGGA6GAUGGGUCCGCAGACGACUCGCCCGA 22'92_H32
TGTTGCTATGGACTGTGCCG GGGAGGACGAUGCGGUGUUGCUAUGGACUGUGCCGCAGACGACUCGCCCGA 2293_H32
CACAGTGAGTGATGCCGGTA GGGAGGACGAUGCGGCACAGU6AGUGAUGCCGGUACAGACGACUCGCCCGA 2294_H32
TTTTGGGGTTTTCCCGTGCCC GGGAGGACGAUGCGGUUUUGGGGUUUUCCCG UGCCCCAGACGACUCGCCCGA 2295 H32 2029 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
T6 TAG G G AG G T AG TTATCCG GGGAGGACGAUGCGGUGUAGGGAGGUAGUUAUCCGCAGACGACUCGCCCGA 2296. .H32
6ATCCTCCTAATTGCGCCGC GGGAGGACGAUGCGGGAUCCUCCUAAUUGCGCCGCCAGACGACUCGCC'CGA 2297. _H3
TTGAAGGGTGATGGGCCCG GGGAGGACGAUGCGGUUGAAGGG UGAUGGGCCCGCAGACGACUCGCCCGA 2298. _H32
GAAGTTGCGCTGCACGTGTC GGGAGGACGAUGCGGGAAG UUGCGCUGCACGUGUCCAGACGACUCGCCCGA 2299 H32
TTGGAAGTGGGATGGGACCG GGGAGGACGAUGCGGUUGGAAGUGGGAUGGGACCGCAGACGACUCGCCCGA 2300. .H32
CTG G ATATACTCCTG ATCCC GGGAGGACGAUGCGGCUGGAUAUACUCCUGAUCCCCAGACGACUCGCCCGA 2301. .H32
TTGGGTGTACGATGGGACCG GGGAGGACGAUGCGGUUGGGUGUAGGAUGGGACCGCAGACGACUCGCCCCA 2302. .H32
CTAACGATGATACAGGTCCA GGGAGGACGAUGCGGCUAACGAUGAUACAGGUCCACAGACGACUCGCCCGA 2303. _H32
AC G G ATG ACTAG ACCCACTG GGGAGGACGAUGCGGACGGAUGACUAGACCCACUGCAGACGACUCGCCCGA 2304. .H32
CATGACCACACTCCTGTCCC 6GGAGGACGAUGCGGCAUGACCACACUCCUG UCCCCAGACGACUCGCCCGA 2305. .H32
A7TGGCGAAGGGCGGTACTG GGGAGGACGAUGCGGAUUGGCGAAGGGCGGUACUGCAGACGACUCGCCGGA 2306. .H32
TTGTCTGTGTGGATTGCGTA GGGAGGACGAUGCGGUUG UCUGUGUGGAUUGCGUACAGACGACUCGCCCGA 2307. .H32
AACATCGTGTCG AAG CTCCC GGGAGGACGAUGCGGAACAUCG UGUCGAAGCUCCCCAGACGACUCGCCCGA 2308. _H32
CTCATCACCATACTGTCGTG GGGAGGACGAUGCGGCUCAUCACCAUACUGUCGUGCAGACGACUCGCCCGA 2309. _H32
AAGTCCAATCTTTTGCGCGC GGGAGGACGAUGCGGAAGUCCAAUCUUUUGCGCGCCAGACGACUCGCCCGA 2310. _H32
TCTCCTAGTAATTTGTCGTC GGGAGGACGAUGCGGUCUCCUAGUAAUUUGUCGUCCAGACGACUCGCCCGA 2311. H32
TCG AG AGTCAG CCACTTCGC GGGAGGACGAUGCGGUCGAGAGUCAGCCACUUCGCCAGACGACUCGCCCGA 2312. JH32
GTTGAAGGGTGATGGGGCCG GGGAGGACGAUGCGGG UUGAAG6GUGAUGGGGCCGCAGACGACUCGCCCGA 2313. _H32 4671 H04
TTG G ATGTAGGGTG GGACCG GGGAGGACGAUGCGGUUG.GAUGUAGGGUGGGACCGCAGACGACUCGCCCGA 2314. .H32
TTTGAAGGGGGATCGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUCGGUCCGCAGACGACUCGCCCGA 2315. _H32
AATGAACGTCCTATGCTG GGGAGGACGAUGCGGAAUGAACGUCCUAUGCUGCAGACGACUCGCCCGA 2316. .H32
TCTCTGGACAACCCGGTTGC GGGAGGACGAUGCGGUCUC'UGGACAACCCGGUUGCCAGACGACUCGCCCGA 2317. ,H32
TAGGCAACATGGACATGCTG GGGAGGACGAUGCGGUAG6CAACAUGGACAUGCUGCAGACGACUCGCCCGA 2318. _H32
TTGGGAGGGGGGTGGGTCCG GGGAGGACGAUGCGGU UGGGAGGGGGGUGGGUCCGCAGACGACUCGCCCGA 2319. .H32
TTG GGGTTGG AGATGG ACTG GGGAGGACGAUGCGGU UGGGGUUGGAGAUGGACUGCAGACGACUCGCCCGA 2320. _H32
TATTGGAGGTGTAGGGGACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAGGGGACCGCAGACGACUCGCCCGA 2321. .H3
CATTCATAGTCTCCCGTGGC GGGAGGACGAUGCGGCAUUCAUAGUCUCCCGUGGCCAGACGACUCGCCCGA 2322. _H32
CG CTAG ACCTACATGCTG CC GGGAGGACGAUGCGGCGCUAGACCUA'CAUGCUGCCCAGACGACUCGCCCGA 2323 H32
# in H4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc
TGCGATGTAAGCTGGTGCGC GGGAGGACGAUGCGGUGCGAUGUAAGCUGGUGCGCCAGACGACUCGCCCGA 2324_H32
TTCCTCTACTCCCTAGCGTA GGGAGGACGAUGCGGUUCCUCUACUCCCUAGCGUACAGACGACUCGCCCGA 2325_H32
ATATCGTCTCCTTCG CTCTG GGGAGGACGAUGCGGAUAUCGUCUCCUUCGCUCUGCAGACGACUCGCCCGA 2326 H32
TATTGGAGATGTAGGGACCG GGGAGGACGAUGCGGUAUUGGAGAUGUAGGGACCGCAGACGACUCGCCCGA 2327_H32
CCATTG TCCTTG ATGT ACCG GGGAGGACGAUGCGGCCAUUGUCCUUGAUGUACCGCAGACGACUCGCCCGA 2328_H32
TCCGAAGG6TGATGGGTCCG G6GAGGACGAUGCGGUCCGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2329.H32 3852_H04
AACCACGAT6CTGCCAGCCG GGGAGGACGAUGCGGAAC ACGAUGCUGCCAGCCGCAGACGACUCGCCCGA 2330_H32
ATTGGAGGAGGGAGGGACCG GGGAGGACGAUGCGGAUUGGAGGAGGGAGGGACCGCAGACGACUCGCCCGA 233 _H32
ATT6GTGGGG6GAGGGACTG G6GAGGACGAUGCGGAUUG6UGGGGGGAGGGACUGCAGACGACUCGCCCGA 2332_H32
GATTCAAGGCCCCGCGTGTG GGGAGGACGAUGCGGGAUUCAAGGCCCCGCGUGUGCAGACGACUCGCCCGA 2333„H32
TGCAGGGAGGTAGTTATCGT GGGAGGACGAUGCGGUGCAGG6A6GUAGU UAUCGUCAGAC6ACUCGCCCGA 2334_H32
TTTGGAGTGGGGTGGTACCG GGGAGGACGAUGCGGUUUGGAGUGGGG UGG UACCGCAGACGACUCGCCCGA 2335_H32
A'fTC GTG CTCATTCCG CCCC GGGAGGACGAUGCGGAU UCGUGCUCAUUCCGCCCCCAGACGACUCGCCCGA 2336_H32
TGTAGGGTGGTAGTTATCGT GGGAGGACGAUGC6GUGUAGGGUGGUAGUUAUCGUCAGACGACUCGCCCGA 2337„H32
AGTTCACAAATCATCT6CTG GGGAGGACGAUGCGGAGLiUCACAAAUCAUCUGCUGCAGACGACUCGCCCGA 2338_H32
TGACTCGTCTGTTTCGTACC GGGAGGACGAUGCGGUGACUCGUCUGUUUCGUACCCAGACGACUCGCCCGA 2339_H32
TTGGAAGTAGGATGGGACCG GGGAGGACGAUGCGGUUGGAAG UAGGAUGGGACCGCAGACGACUCGCCCGA 2340_H32
TI GAAGGGTGATGGGTCCGC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUCCGCCAGACGACUCGCCCGA 2341_H32
TTGGGTGTAGGGCGCTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGCGCUACCGCAGACGACUCGCCCGA 2342_H32
TTGG GTAG G GTG GTACTG GGGAGGACGAUGCGGUUGGGUAGGG UGGUACUGCAGACGACUCGCCCGA 2343_ H32
1TTGAAGGGTGATGGGTCCGG GGGAGGACGAUGCGGU U UGAAGGGUGAUGGG UCCGGCAGACGACUCGCCCGA 2344_H32
TTGGGTGTAGGGAGGTACCC GGGAGGACGAUGCGGUUGGGUGUAGGGAGG UACCCCAGACGACUCGCCCGA 2345_H32
TTTGAAG GTTG ATG GTTCCG GGGAGGACGAUGCGGUU UGAAGGUUGAUGGUUCCGCA6ACGACUC6CCCGA 2 46JH32
TTTGAAGGGGGATGGGTCCA GGGAGGACGAUGCGGUUUGAAGGGGGAUGGGUCCACAGACGACUCGCCC A 2347_H32 3S32_H04
TTTGAAG CG C G ATG G G TCCG GGGAGGACGAUGCGGUUUGAAGCGCGAUGGGUCCGCAGACGACUCGCCCGA 2 48_H32 4251JHQ4
TTTGCAGGGTGGTGGGTCCG GGGAGGACGAUGCGGUUUGCAGGGUGGUGGG UCCGCAGACGACUCGCCCGA 2349_H32 4490_H04
ATTG G AG G GGG GAG GGACTG GGGAGGACGAUGCGGAUUGGAGGGGGGAGGGACUGCAGACGACUCGCCCGA 235G - H32
TTGGGAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUGGGAGGGUGAUGGGUCCGCAGACGACUCGCCC6A 2351 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTG CAG G G TG CTG G GTCCG GGGAGGACGAUGCGGU UUGCAGGGUGCUGGGUCCGCAGACGACUCGCCCGA 2352_ H32 41 S_H04
TTTGAAG GGTG TTGGGTCCC GGGAGGACGAUGC6GU UUGAAGGGUGU UG6GUCCCCAGACGACUCGCCCGA 2353. H32 2264 H04
TTTGG GTGTAGG GTG GTACCT GGGAGGACGAUGCGGU UUGGGUG UAGGGUGGUACCUCAGACGACUCGCCCGA 2354. H32
TGTCGGAGGAGGAGGTACCG GGGAGGACGAUGCGGUGUCGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 2355. .H32
TCATCGTTGTCCTTGGTCCC GGGAGGACGAUGCGGUCAUCGUUGUCCUUGGUCCCCAGACGACUCGCCCGA 23S5_ .H32
CCACTGCAAGAATGGTGCT6 GGGAGGACGAUGCGGCCACUGCAAGAAUGG UGCUGCAGACGACUCGCCCGA 2357. H32
CTTGTCGTCTTG GTC6TCCC GGGAGGACGAUGCGGCUUG UCGUCUUGGUCGUCCCCAGACGACUCGCCCGA 2358. .H32
GTTG A AGTGTCCGTACTG TG GGGAGGACGAUGCGGGUUGAAGUGUCCGUACUGUGCAGACGACUCGCCC6A 2359_ H32
AGAGTTTCGTCAGCCGTGTG GGGAGGACGAUGCGGAGAGUU UCG UCAGCCGUGUGCAGACGACUCGCCCGA 2360. H32
GAGTCCGTCAATGTTGTGTC GGGAGGACGAUGCGGGAGUCCGUCAAUGUUGUGUCCAGACGACUCGCCCGA 2361. H32
ACCGAACACAATAAGTACCG GGGAGGACGAUGCGGACCGAACACAAUAAGUACCGCAGACGACUCGCCCGA 2362_ _H32
GTGTGCCGATTTGGCATGGC GGGA6GACGAUGCGGG UGUGCCGAUUUGGCAUGGCCAGACGACUCGCCCGA 2363. H32
CGAATCGTACTACCGTGCAC GGGAGGACGAUGCGGCGAAUCGUACUACCGUGCACCAGACGACUCGCCCGA 2364_ .H32
CCATCAATGTAAGGGGTGAC GGGAGGACGAUGCGGCCAUCAALiGUAAGGGGUGACCAGACGACUCGCCCGA 2365. H32
AACGAGAAGTCTACTCCCCC GGGAGGACGAUGCGGAACGAGAAGUCUACUCCCCCCAGACGACUCGCCCGA 2366. .H32
TGTCGTAGTGGCGTTGTGGC GGGAGGACGAUGCGGUGUCGUAGUGGCGUUGUGGCCAGACGACUCGCCCGA 2367. H32
AGTCGTGGTTCCCGCGTCAT GGGAGGACGAUGCGGAGUCGUGGUUCCCGCGUCAUCAGACGACUCGCCCGA 2368. .H32
ACCAGCCAGTACAGCGCTGC GGGAGGACGAUGCGGACCAGCCAG UACAGCGCUGCCAGACGACUCGCCCGA 2369. H32
TTTGAAGGGTGGTGGCTCCG GGGAGGACGAUGCGGU UUGAAGGGUGGUGGCUCCGCAGACGACUCGCCCGA 2370. .H32
TCG GTACAG TTG CTCTGCTG G G GAGG ACG AU G CG G U CGG U AC AG U U G C U CU GCU G CAG ACG AC UCG CCCGA 2371. .H32
TCTCCGATGCATGTAGCCGT GGGAGGACGAUGCGGUCUCCGAUGCAUGUAGCCGUCAGACGACUCGCCCGA 2372. _H32
ATGTATGTGGGAGCCACGC GGGAGGACGAUGCGGAUGUALJGUGGGAGCCACGCCAGACGACUCGCCC6A 2373. H32
AG CTGTAATTC TGTCTGTG GGGAGGACGAUGCGGAGCUG UAAUUCAUG UCUGUGCAGACGACUCGCCCGA 2374. H32
TTC7TCTCCCAACGTGTGTA GGGAGGACGAUGCGGUUCUUCUCCCAACGUGUGUACAGACGACUCGCCCGA 2375. H32
AACCCCTTCCTCGTCTCGTC GGGAGGACGAUGCGGAACCCCUUCCUCGUCUCGUCCAGACGACUCGCCCGA 2376. .H32
CTTCAACACCTCTTCGTCTG GGGAGGACGAUGCGGCUUCAACACCUCU UCGUCUGCAGACGACUCGCCCGA 2377. H32
CATACGTCTAACCCGTGCCC GGGAGGACGAUGCGGCAUACGUCUAACCCGUGCCCCAGACGACUCGCCCGA 2378. .H32
CACTAAGGTTGACCGGTAJC GGGAGGACGAUGCGGCACUAAGG UUGACCGGUAUCCAGACGACUCGCCCGA 2379. .H32
# in H4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc
ATTTCTACCTATA6CTTCT6 GGGAGGACGAUGCGGAUUUCUACCUAUAGCUUCUGCAGACGACUCGCCCGA 2380_ .H32
TCTGAAGGGTGATGGGTCCC GGGAGGACGAUGCGGUCUGAAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 2381_ .H32
CTTG GCGTCTTGTTCG TCCC GGGAGGACGAUGCGGCUUGGCGUCUUGUUCG UCCCCAGACGACUCGCCCGA 2382. ,H32
ATAGGGAGAAGTGTGAACCG GGGAGGACGAUGCGGAUAGGGAGAAGUG UGAACCGCAGACGACUCGCCCGA 2383_ _H32
TAATCTGTCCAACGCGTGCC GGGAGGACGAUGCGGUAAUCUGUCCAACGCGUGCCCAGACGACUCGCCCGA 2384_ .H32
ATCCGGCATTCCTCCGTGTG GGGAGGACGAUGCGGAUCCGGCAUUCCUCCG UGUGCAGACGAGUCGCCCGA 2385_ .H32
ATTGGTGAGGG GAGGGACCG GGGAGGACGAUGCGGAUUGGUGAGGGGAGGGACCGCAGACGACUCGCCCGA 23S6„ .H32
CCAATGAATCCTGTACCGCA GGGAGGACGAUGCGGCCAAUGAAUCCUGUACCGCACAGACGACUCGCCCGA 238 ^ ^H32
TTGGGTGTAGG GTGG AACTG GGGAGGACGAUGCGGU UGGGUGUAGGGUGGAACUGCAGACGACUCGCCCGA 238S_ _H32
TTTCTCCCACG CTGTG TTG C GGGAGGACGAUGCGGU UUCUCCCACGCUGUGUUGCCAGACGACUCGCCCGA 238 _ H32
GG TTCTACTG 6TCACCGTGC GGGAGGACGAUGCGGGGU UCUACUGGUCACCGUGCCAGACGACUCGCCCGA 2390_ H32
ACTiTTGCGATATG TAGCCG GGGAGGACGAUGCGGACUUUUGCGAUAUGUAGCCGCAGACGACUCGCCCGA 2391_ .H32
ATCG G G TA G CCGTCCAGTGC GGGAGGACGAUGCGGAUCGGGUAGCCGUCCAGUGCCAGACGACUCGGCCGA 2392_ _H32
4- TCG CATTCGATTGTTCCCCC GGGAGGACGAUGCGGUCGCAUUCGAUUGUUCCCCCCAGACGACUCGCCCGA 2393_ _H32
TCTAGTCTTGGATGGTGCGC GGGAGGACGAUGCGGUCUA UCUUGGAUGGUGCGCCAGACGACUCGCCCGA 2394, .H32
GTTGAACGGGCAACCTACCC GGGAGGACGAUGCGGGUUGAACGGGCAACCUACCCCAGACGACUCGCCCGA 2 95_ _H32
ATCGCATGTCTGTCGCTGCC G6GAGGACGAUGCGGAUCGCAUGUCUGUCGCUGCCCAGACGACUCGCCCGA 2396_ .H32
ATTAATGAAGGGAGGTACTG G6GAGGACGAUGCGGAUUAAUGAAGGGAGGUACUGCAGACGACUCGCCCGA 2397. .H32
TG A G AACTCCAC GTGC G CTC GGGAGGACGAUGCGGUGAGAACUCCACGUGCGCUCCAGACGACUCGCCCGA 239S__ ,H32
ATGTCCCCATCTTCGTCCCC GGGAGGACGAUGCGGAUGUCCCCAUCUUCGUCCCCCAGACGACUCGCCCGA 2399. .H32
C ATTG TG CTC6 TTTT6 C CCC GGGAGGACGAUGCGGCAUUGUGCUCGUUUUGCCCCCAGACGACUCGCCCGA 2400. .H32
ATCGTGTGGCTGTGTGTGGC GGGAGGACGAUGCGGAUCGUG UGGCUGUGUGUGGCCAGACGACUCGCCCGA 2401_ ^H32
TATCACTCAAAGTCTG CCGC GGGAGGACGAUGCG6UAUCACUCAAAGUCUGCCGCCAGACGACUCGCCCGA 2402_ .H32
TGCATGCTAGAACATGTGTG GGGAGGACGAUGCGGUGCA.UGCUAGAACAUGUGUGCAGACGACUCGCCCG 2403_ „H32
TTGTCCTGCATAACTGTGCC GGGAGGACGAUGCGGU UGUCCUGCAUAACUGUGCCCAGAC6ACUCGCCCGA 2404_ _H32
CTCCCCATGAGTGTCTTGTG GGGAGGACGAUGCGGCUCCCCAUGAGUGUCUUGUGCAGACGACUCGCCCGA 24Q5_ _H32
ACGAGAGCCATCTGTAGGTG GGGAGGACGAUGCGGACGAGAGCCAUCUGUAGGUGCAGACGACUCGCCCGA 2406_ .H32
GTATGTAATCTGCCGTTCTG GGGAGGACGAUGCGGG UAUGUAAUCUGCCGUUCUGCAGACGACUCGCCCGA 2407 H32
# in H4
NTS read variable region RNA sequences U in H3 selection selection Selected sequenc
GACGACGAGTTTGCCGCATG CGCACGACGAUGCGGGACGACGAGUUUGCCGCAUGCAGACGACUCGCCCGA 2408. .H32
CTTGGTGTAGGGTGGTACCG GGGAGGACGAUGCGGCUUGGUGUAGGGUGGUACCGCAGACGACUCGCCCGA 2409. _H32
TGTAGGAGGAGGAGGTCCCG GGGAGGACGAUGCGGUGUAGGAGGA6GAGGUCCCGCAGACGACUCGCCCGA 2410. _H32
ATCGTCG AGTG ATCTG CCCG GGGAGGACGAUGCGGAUCGUCGAGUGAUCUGCCCGCAGACGACUCGCCCGA 2411. _H32
CTG CACAA AATAGG CTG CAC GGGAGGACGAUGCGGCUGCACAAAAUAGGCUGCACCAGACGACUCGCCCGA 2412. _H32
ATTG CTGG GATTTTTG G CCG GGGAGGACGAUGCGGAUUGCUGGGAUUUUUGGCCGCAGACGACUCGCCCGA 2413. _H32
TGTCTAGTAATTGGTGTCTG GGGAGGACGAUGCGGUGUCUAGUAAUUGGUGUCUGCAGACGACUCGCCCGA 2414. .H32
AAACTCCGTCTTCCACGCGC GGGAGGACGAUGCGGAAACUCCGUCUUCCACGCGCCAGACGACUCGCCCGA 2415. .H32
AAGTGTCCTCTTACTGGTGC GGGAGGACGAUGCGGAAGUGUCCUCUUACUGGUGCCAGACGACUCGCCCGA 2416. _H32
TCCCCCAATGTCGCTGTGGC GGGAGGACGAUGCGGUCCCCCAAUGUCGCUG UGGCCAGACGACUCGCCCGA 2417. _H32
GTTGAAGAGTGATGGGTCCG GGGAGGACGAUGCGGGUUGAAGAGUGAUGGGUCCGCAGACGACUCGCCCGA 2418. _H32
TTTCG CTATTCCTG ACTCGC GGGAGGACGAUGCGGU UUCGCUAUUCCUGACUCGCCAGACGACUCGCCCGA 2419. _H32
CG AG CATCTGTCAACG GTAC GGGAGGACGAUGCGGCGAGCAUCUGUCAACGGUACCAGACGACUCGCCCGA 2420. _H32
•J\ ATCATCATCCTTTGCGTGG C GGGAGGACGAUGCGGAUCAUCAUCCUU UGCGUGGCCAGACGACUCGCCCGA 2421. H32
TGACTCCACTGTACT6TCCC GGGAGGACGAUGCGGUGACUCCACUGUACUGUCCCCAGACGACUCGCCCGA 2422. . H32
CAACGTCCAAAATGTAGGTC GGGAGGACGAUGCGGCAACGUCCAAAAUGUAGGUCCAGACGACUCGCCCGA 2423. .H32
GACGAGCATACCACGGTCCC GGGAGGACGAUGCGGGACGAGCAUACCACGG UCCCCAGACGACUCGCCCGA 2424. _H32
TTCTCCACACGACTCGTCTG G6GAGGACGAUGCGGUUCUCCACACGACUCGUCUGCAGACGACUCGCCCGA 2425. _H32
CAA7CGTCTG TTGTTG GTG C GGGAGGACGAUGCGGCAAUCGUCUGUUGUUGGUGCCAGACGACUCGCCCGA 2425. _H32
AATACGGACGAATCCTCGTA GGGAGGACGAU6CGGAAUACGGACGAAUCCUCGUACAGACGACUCGCCCGA 2427, .H32
AG TCTGTCTGT AC AATGCTG GGGA6GACGAUGCGGAGUCUGUCUGUACAAUGCUGCAGACGACUCGCCCGA 2428. „H32
CTATCAGTCAACGTGGTGCC GGGAGGACGAUGCGGCUAUCAGUCAACGUGGUGCCCAGACGACUCGCCCGA 2429. .H32
AAGATGATCGTCACAGTATG GGGAGGACGAUGCGGAAGAUGAUCGUCACAGUAUGCAGACGACUCGCCCGA 2430. _H32
GTTCGGTCTGATTCCTCeCC GGGAGGACGAUGCGGGUUCGG UCUGAUUCCUCCCCCAGACGACUCGCCCGA 2431. .H32
TATCTACTTATTGAAGT6CC GGGAGGACGAUGCGGUAUCUACUUAUUGAAG UGCCCAGACGAC UCGCCCGA 2432. _H32
TGTCTTG G AA AACTG G TCCG GGGAGGACGAUGCGGUGUCUUGGAAAACUG6UCCGCAGACGACUCGCCCG 2433. _H32
ACAAGCTGTAACACCGCGTG GGGAGGACGAUGCGGACAAGCUG UAACACCGCGUGCAGACGACUCGCCCGA 2434, „H32
CAGTCATCCATCTGGTCGTA GGGAGGACGAUGCGGCAGUCAUCCAUCUGG UCGUACAGACGACUCGCCCGA 2435 H32
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
ATTGTCT6TG GTG CTAG CCC GGGAGGACGAUGCGGAUUGUC UG UGGUGCUAGCCCCA6ACGACUCGCCCGA 2436„H32
CTGTGAGGAGCAGGGTGGTG GGGAGGACGAUGCGGCUGUGAGGAGCAGGGUGGUGCAGACGACUCGCCCGA 2437_,H32
TATTGGAGGTGTAGGGACAG GGGAGGACGAUGCGGUAU UGGAGGUGUAGGGACAGCAGACGACUCGCCCGA 2438_H32
GAG ATTG TACGG GTGTG CCC GGGAGGACGAUGCGGGAGAUUGUACGGGUG UGCCCCAGACGACUCGCCCGA 2439_H32
ATCGTG ATG GATGCCG TGCC GGGAGGACGAUGCGGAUCG UGAUGGAUGCCGUGCCCAGACGACUCGCCCGA 2440_H32
TCTGAAGGGG ATG GGTCCG G6GAGGACGAUGCGGUCUGAAGGGGGAUGGGUCCGCAGACGACUCGCCCGA 2441JH32
TTGGGTGTCGGGCGGTACCG GGGAGGACGAyGCGGUUGGGUGUCGeGCGG UACCGCAGACGACUCGCCCGA 2442_H32
TTTCGTTCCTCTGTGCGCTG GGGAGGACGAUGCGGUUUCGUUCCUCUGUGCGCUGCAGACGACUCGCCCGA 2443_H32
TTTTAAGGGTTATGGGTCCG G6GAGGACGAUGCGGUUU UAAGGGUUAUGGGUCCGCAGACGACUCGCCCGA 2444_H32
TTGGGTGTAGGTGGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGUGGGUACCGCAGACGACUCGCCCGA 2445_H32
TTTG AAG TAG G G To G T ACCG GGGAGGACGAUGCGGUUUGAAGUAGGGUGGUACCGCAGACGACUCGCCCGA 2446_H32
TATTGGAGGTGTAGGGATCG GGGAGGACGAUGCGGUAU UGGAGGUGUAGGGAUCGCAGACGACUCGCCCGA 2447_H32
TTGGGAGAAGGGTGGTACCG GGGAGGACGAUGCGGUUGGGAGAAGGGUGGUACCGCA6ACGACUCGCCCGA 2448_,H32
CCGTTTAACCGTCCTGTCCC GGGAGGACGAUGCGGCCGUUUAACCGUCCUGUCCCCAGAC6AGUCGCCCGA 2449_ H32
ATTGGAGGGTGATGGGTCCG GGGAGGACGAUGCGGAUUGGAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 2450„H32
TTAGTCGTTGTTTGCCTGTG GGGAGGACGAUGCGGUUAGUCGUUGUU UGCCUGUGCAGACGACUCGCCCGA 2451_H32
TTTGTTCTCCTTTGGCTCAC GGGAGGACGAUGCGGUUUGUUCUCCUUUGGCUCACCAGACGACUCGCCCGA 2452_H32
TGACTCGTCTGTTTCATCCC GGGAGGACGAUGCGGUGACUCGUCUGUUUCAUCCCCAGACGACUCGCCCGA 2453_H32
TGGCTCGATGTCCTGTACCC GGGAGGACGAUGCGGUGGCUCGAUGUCCUGUACCCCAGACGACUCGCCCGA 2454_H32
ATCGTTTG6CTGTTGTGCAC GGGAGGACGAUGCGGAUCGUUUGGCUGUUGUGCACCAGACGACUCGCCCGA 2455_H32
TCCTTG TC GTCTTGTGTCCC GGGAGGACGAUGCGGUCCU UGUCGUCUUGUGUCCCCAGACGACUCGCCCGA 2456_ H32 2924 H04
TCTGG CG AACATAGACTGCC GGGAGGACGAUGCGGUCUGGCGAACAUAGACUGCCCAGACGACUCGCCCGA 2457_H32
CATAGTAGACTTGACCTCCC GGGAGGACGAUGCGGCAUAGUAGACUUGACCUCCCCAGACGACUCGCCCGA 2458_H32
CAGTTGTCTTCACTCCCCCC GGGAGGACGAUGCGGCAGUUG UCU UCACUCCCCCCCAGACGACUCGCCCGA 2459_H32
CAAG TG A ACGTCTG AG CCCC GGGAGGACGAUGCGGCAAGUGAACG UCUGAGCCCCCAGACGACUCGCCCGA 246Q_H32
ACCGCACGTCTTTATGTCGT GGGAGGACGAUGCGGACCGCACG UCU UUAUGUCGUCAGACGACDCGCCCGA 2461_H32
AT ATTG TAG GTCTG CATGTG GGGAGGACGAUGCGGAUAUUG UAGG UCUGCAUG UGCAGACGACUCGCCCGA 2462_H32
TGTTCGCGTGTGCTTTTGCC GGGAGGACGAUGCGGUGUUCGCG UGUGCUU UUGCCCAGACGACUCGCCCGA 2463 H32
# in H4
HTS read variable region RNA sequences » in H3 selection selection Selected sequenc
AGATGCCGCCCTTCCGTGTG GGGAGGACGAUGCGGA6AUGCC6CCCUUCCGUG UGCAGACGACUCGCCCGA 2464_ _H32
TG CTTGG ACTT ATGTCGGTA GGGAGGACGAUGCGGUGCU UGGACUUAUGUCGGUACAGACGACUCGCCCGA 2465. _H32
TAACATTCCTA7GCG CTCTG GGGAGGACGAUGCGGUAACAUUCCUAUGCGCUCUGCAGACGACUCGCCCGA 2466. ^H32
TGAACGTAAAAGTTCATGTG GGGAGGACGAUGCGGUGAACGUAAAAGUUCAUGUGCAGACGACUCGCCCGA 2467. .H32
TIG TCTAGTTTGGGTGCTAC GGGAGGACGAUGCG6UUGUCUAGUUUGGGUGCUACCAGACGACUCGCCCGA 2468. _H32
ATGCCCGACGATGTCCCGTA GGGAGGACGAUGCGGAUGCCCGACGAUGUCCCGUACAGACGACUCGCCCGA 2469. _H32
GACCGTGTCAGGATTGCCCT GGGAGGACGAUGCGGGACCGUG UCAGGAU UGCCCUCAGACGACUCGCCCGA 2470. _H32
TTTGAAG GGTAATGGGTCCC GGGAGGACGAUGCGG U UUGAAGGGUAAUGGGUCCCCAGACGACUCGCCCGA 2471. .H32 2563 H04
TAAGTAGTCTGTATGCG GTC GGGAGGACGAUGCGGUAAG UAGUCUGUAUGCGGUCCAGACGACUCGCCCGA 2472. .H32
TCTC CCA C A A AGTTTG G CTG GGGAGGACGAUGCGGUCUCCCACAAAGU UUGGCUGCAGACGACUC6CCCGA 2473. _H32
CACGTCTTAATTTGCCCCCC GGGAGGACGAUGCGGCACGUCUUAAUUUCCCCCCCCAGACGACUCGCCCGA 2474. _H32
TAACA T ACG TCT G GCCGCTG GGGAGGACGAUGCGGUAACAUACGUCUGGCCGCUGCAGACGACUCGCCCGA 2475. .H32
TTTGAAGGGTGATGGGTGGG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUGGGCAGACGACUCGCCCGA 2476. H32
TCCCCTTCGTTTTG CTG CCG GGGAGGACGAUGCGGUCCCCUUCGUU UUGCUGCCGCAGACGACUCGCCCGA 2477. _H32
ATCAG GT6CTCTG TC GTGTA GGGAGGACGAUGCGGAUCAGGUGCUCUGUCGUGUACAGACGACUCGCCCGA 2478. .H32
CCGCTTCTCGTGTTACCCGC GGGAGGACGAUGCGGCCGCUUCUCGUGUUACCCGCCAG'ACGACUCGCCCGA 2479. _H32
CATCGACTGTAGTTTATGTG GGGAG6ACGAUGCGGCAUCGACUGUAGUUUAUGUGCAGACGACUCGCCCGA 2480. _H32
AGTGTCGGATCTGGTGTT6C GGGAGGACGAUGCGGAGUGUCGGAUCUGGUGUUGCCAGACGACUCGCCCGA 2481. _H32
CGATTGTTGGGGATCTGCCC GGGAGGACGAUGCGGCGAUUG UUGGGGAUCUGCCCCAGACGACUCGCCCGA 2482.. ,H32
AATCG'CTC CC A ATTG C G TG C GGGAGGACGAUGCGGAAUCGCUCCCAAUUGCGUGCCAGACGACUCGCCCGA 2483. .H32
TATCATACGAATACGTGTG GGGAGGACGAUGCGGUAUCAUACGAAUACG UG UGCAGACGACUCGCCCGA 2.484. _H32
CAGTAAGGTCGGTGCGTGTC GGGAGGACGAUGCGGCAGUAAGGUCGGU6CGUGUCCAGACGACUCGCCCGA 2485. .H32
ATCCTCGTAATTGCCGCGTA GGGAGGACGAUGCGGAUCCUCGUAAUUGCCGCG UACAGACGACUCGCCCGA 2486. .H32
TTTGGTGAAGGGGGGTACTG GGGAGGACGAUGCGGUUUGGUGAAGGGGGGUACUGCAGACGACUCGCCCGA 2487. _H32
TCCTTGTCGTTTGCGTCCC GGGAGGACGAUGCGGUCCUUGUCGUUUGCGUCCCCAGACGACUCGCCCGA 2488. .H32 1743 H04
AGAGTCTGATGTCACCTGTG GGGAGGACGAUGCGGAGAGUCUGAUGUCACCUG JGCAGACGACUCCCCCGA 2489. .H32
GCGGTACG ATCTA ATCTCCC GGGAGGACGAUGCGGGCGGUACGAUCUAAUCUCCCCAGACGACUCGCCCGA 2490. .H32
G CAGCG CT AGTTCTCGTG AC GGGAGGACGAUGCGGGCAGCGCUAGUUCUCGUGACCAGACGACUCGCCCGA 2491 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AATGAGTCTGACCGTCACGC GGGAGGACGAUGCGGAAUGAGUCUGACCG UCACGCCAGAC6ACUCGCCCGA 2492_ _H32
GTTGAGGGGTGATGGGTCCG GGGAGGACGAUGCGGG UUGAG6GGUGAUGGG UCCGCAGACGACUCGCCCGA 2493. _H32 3710_H04
GCCTTTCGTTCCCCTCCC GGGAGGACGAUGCGGGCCU U UCGU UCCCCUCCCCAGACGACUCGCCCGA 2494_ _H32 3846 H04
TTTGAAGGGTTATGGGCCCG GGGAGGACGAUGCGGU UUGAAGGGUUAUGG6CCCGCAGACGACUC6CCCGA 2495. .H32
GTTGGTGGAGGGAGGTACTG GGGAGGACGAUGCGGGUUGGUGGAGGGAGGUACUGCAGACGACUCGCCCGA 2496. .H32
AATGAATTATCTACATTCCC GGGAGGACGAUGCGGAAUGAAUUAUCUACAUUCCCCAGACGACUCGCCCGA 2497. .H32
TCATGCGATAGTCTCCCCCC GGGAGGACGAUGCGGUCAUGCGAUAGUCUCCCCCCCAGACGACUCGCCCGA 2498. _H32
TTCTTCCCTTGTTG CGGGTA GGGAGGACGAUGCGGU UCUUCCCUUGUUGCGGGUACAGACGACUCGCCCGA 2499„ -H32
CCCGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGCCCGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 2500. .H32
AACCCTGTG CACATGCCGTA GGGAGGACGAUGCGGAACCCUGUGCACAUGCCGUACAGACGACUCGCCCGA 2501. .H32
ATATACGTACATGGGTGGCC GGGAGGACGAUGCGGAUAUACGUACAUGGGUGGCCCAGACGACUCGCCCGA 2502. _H32
GTC CT6TG CGCCTGTGTGAC GGGAGGACGAUGCGGGUCCUGUGCGCCUG UGUGACCAGACGACUCGCCCGA 2503. _H32
TAGGTGCAAATCCACAGCCC GGGAGGACGAUGCGGUAGGUGCAAAUCCACAGCCCCAGACGACUCGCCCGA 2504_ _H32
AATCTTG AC C CCTCG CCATG GGGAGGACGAUGCGGAAUCU UGACCCCUCGCCAUGCAGACGACUCGCCCGA 2505. _H32
ACCGTATCATC ATG CCGCC A GGGAGGACGAUGCGGACCGUAUCAUCAUGCCGCCACAGACGACUCGCCCGA 2506. _H32
ATTGGAGGAGGGAGGTACTG GGGAGGACGAUGCGGAUUGGAGGAGGGAGGUACUGCAGACGACUCGCCCGA 2507. _H32
TCTGTGTGGGATGTTGCCC GGGAGGACGAUGCGGUCUGUGUGGGAUGUUGCCCCAGACGACUCGCCCGA 2508. _H32
CCTATCGTCATGTTCGTGGC GGGAGGACGAUGCGGCCUAUCGUCAUGUUCGUGGCCAGACGACUCGCCCGA 2509. .H32
CATAG GACAGG GTTGTG GCC GGGAGGACGAU6CGGCAUAGGACAGGGUUGUGGCCCAGACGACUCGCCCGA 2510. .H32
CCTTCATTGTCTTCGCGGTA GGGAGGACGAUGCGGCCUUCAUUGUCUUCGCGGUACAGACGACUCGCCCGA 2511. _H32
GCTATGTGTGTTCGTTGGGC GGGAGGACGAUGCGGGCUAUGUGUGUUCGU UGGGCCAGACGACUCGCCCGA 2512. _H32
TCCTGTTGCCCCATGTGTGC GGGAGGACGAUGCGGUCCUG UUGCCCCAUGUG UGCCAGACGACUCGCCCGA 2513. _H32
ACCAGCGTATGTACACTCCC GGGAGGACGAUGCGGACCAGCGUAUGUACACUCCCCAGACGACUCGCCCGA 2514. .H32
TGTAGGAGGTGTAGGGACCG GGGAGGACGAUGCGGUGUAGGAGGUGUAGGGACCGCAGACGACUCGCCCGA 2515. .H32
GTTG ATG CTG GTC A CG ATGC G6GAGGACGAUGCGGGUUGAUGCUGGUCACGAUGCCAGACGACUCGCCCGA 2516. .H32
TGTCCG TTCCCG ATCTGTG C GGGAGGACGAUGCGGUGUCCGUUCCCGAUCUG UGCCAGACGACUCGCCCGA 2517. _H32
AGTGTGAGTCGTTGTACCCC CGGAGGACGAUGCGGAGUGUGAGUCGUUGUACCCCCAGACGAQJCGCCCGA 2518. „H32
AG TAGCG CCTGCTG ACTGCC GGGAGGACGAUGCGGAGUAGCGCCUGCUGACUGCCCAGACGACUCGCCCGA 2519 H32
# in H4
HTS read varia le region RNA sequences # in H3 selection selection Seiected sequenc
GTGAGCATCTGACCCCT6TC GGGAGGACGAUGCGGGUGAGCAUCUGACCCCUG UCCAGACGACUCGCCCGA 2520_H32
TATAATCCTGTGCCTGACCC GGGAGGACGAUGCGGUAUAAUCCUGUGCCUGACCCCAGACGACUCGCCCGA 2521_H32
GGAACCTCACAGTATGTCCG GGGAGGACGAUGCGGGGAACCUCACAGUAUGUCCGCAGACGACUCGCCCGA 2522_H32
GTTCACGATTAGCGCCTGGC G6GAGGACGAUGCGGGUUCACGAUUAGCGCCUG6CCAGAC6ACUCGCCCGA 2523_H32
CGTATTGTCTCGCCAGTGTG GGGAGGACGAUGCGGCGUAUUGUCUCGCCAGUGUGCAGACGACUCGCCCGA 2524_H32
GTGTCTTCTGA6TTTTGGCC GGGAGGACGAUGCGGGUGUCUUCUGAGUUU UGGCCCAGACGACUCGCCCGA 2525__H32
TTTGAAGGGGGATGGGTTCG GGGA6GACGAUGCGGUUUGAAGGGGGAUGGGU UCGCAGACGACUCGCCCGA 2526„H32 4926 H04
TTTGAAGATTGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGAUUGAUGGGUCCGCAGACGACUCGCCCGA 2527_H32
TTTGAAGGCAGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGCAGAUGGG UCCGCAGACGACUCGCCCGA 2528_H32
TTTGAAGGGCGATGGGTACG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGUACGCAGACGACUCGCCCGA 2529_H32 4253_H04
TTTGTGGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUG UGGGGU6AUGGGUCCGCAGACGACUCGCCCGA 2530_H32 3626 H04
TTGGGTGTAGGGGGGTACCC GGGAGGACGAUGCGGUUGGGUG UAGGGGGG UACCCCAGACGACUCGCCCGA 2531_H32
ATT6GTGGAGGGTGGTACTG GGGAGGACGAUGCGGAU UGGUGGAGGGUGG UACUGCAGACGACUCGCCCGA 2532_H32
ATTGGTGCAGGGAGGCACTG GGGAGGACGAUGCGGAUUGGUGCAGGGAGGCACUGCAGACGACUCGCCCGA 2533_H32
TTTGAAGGGTGATGGGGCGG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGGCGGCAGACGACUCGCCCGA 2534_H32
GTGGGTGTAGGGGGGTACCG GGGAGGACGAUGCGGGUGGGUGUAGGGGGGLiACCGCAGACGACUCGCCCGA 2535„H32
TTTGAAGGTTGATGGGTCCC GGGAGGACGAUGCGGUUUGAAGGU UGAUGGGUCCCCAGACGACUCGCCCGA 2536_H32 4365 H04
TTTGAAGGGTGGTGGGTTCG GGGAGGACGAUGCGGUUUGAAGGG UGGUGGGUUCGCAGACGACUCGCCCGA 2537_H32
TTGGGTGTAGAATGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGAAUGGUACCGCAGACGACUCGCCCGA 2538_H32
TTTGAACGGAGATGGGTCCG GGGAGGACGAUGCGGUUUGAACGGAGAUGGGUCCGCAGACGACUCGCCCGA 2539_H32 4252 H04
ATCGAAGGATTTTTGGGCTG GGGAGGACGAUGCGGAUCGAAGGAUUUUUGGGCUGCAGACGACUCGCCCGA 2540_H32
TTTGAAGGGTGATGATGTCCG GGGAGGACGAUGCGGUUUGAAGGG UGAUGAUGUCCGCAGACGACUCGCCCGA 2541_H32 5100 H04
TATTTGAGGTGTAGGGACCG GGGAGGACGAUGCGGUAUU UGAGGUGUAGGGACCGCAGACGACUCGCCCGA 2542_H32
TGTAGGGAGGTAGCTATCGT GGGAGGAC6AUGCGGUG UA6GGAGGUAGCUAUCGUCAGACGACUCGCCCGA 2543_H32
TTGGGTGTAGGGCTGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGCUG UACCGCAGACGACUCGCCCGA 2544_H32
TAGCCTTCATTGTTGCCCCC GGGAGGACGAUGCGGUAGCCUUCAUUGUUGCCCCCCAGACGACUCGCCCGA 2545_H32
AC AG G G AG AAG G G TG A ACCG GGGAGGACGAUGCGGACAGGGAGAAGGGUGAACCGCAGACGACUCGCCCGA 2546_H32
TTTG AATG GIG ATG GG TCTG GGGAGGACGAUGCGGUU UGAAUGG UGAUGGGUCUGCAGACGACUCGCCCGA 2547 H32
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TCCTGTCGTCTTTGCGTTCC GGGAGGACGAUGCGGUCCUGUCGUCUUUGCGUUCCCAGACGACUCGCCCGA 2548_ _H32
CGG6GTGTAGGGTGGTACCG GGGAGGACGAUGCGGCGGGGUGUAGGGUGG UACCGCAGACGACUCGCCCGA 2549_ ,H32
CTGGTG AAGG GAG GTACTG GGGAGGACGAUGCGGCUGG UGAAGGGAGGUACUGCAGACGACUCGCCCGA 2550. .H32
TTTGAAGGGTGATTGGTCCC GGGAGGACGAUGCGGUUUGAAGGGUGAUUGGUCCCCAGACGACUCGCCCGA 2551. .H32 3264 H04
TTGGGTGAGGGTGGTACCG G6GAGGACGAUGCGGUUGGGUGAGGGUGGUACCGCAGACGACUCGCCC6A 2552, _H32
GTGACAAACTAGGTGGGCGC GGGAGGACGAUGCGGGUGACAAACUAGGUGGGCGCCAGACGACUCGCCCGA 2553, .H32
CCA6GTCGTTCTACTCACCC GGGAGGACGAUGCGGCCAGGUCGUUCUACUCACCCCAGACGACUCGCCCGA 2554. .H32
TGCCGATTAAGTCTGGTTGC GGGAGGACGAUGCGGUGCCGAliUAAGUCUGGUUGCCAGACGACUCGCCCGA 2555. .H32
TCAGCTGGAGGTTGCCCCTA GGGAGGACGAUGCGGUCAGCUGGAGGUUGCCCCUACAGACGACUCGCCCGA 2556. .H32
GAGGTAATGTCTGGGTGCTC GGGAGGACGAUGCGGGAGGUAAUGUCUGGGUGCUCCAGACGACUCGCCCGA 2557.. . H32
CATCCGTTGTGTCATGTCGG G6GAGGACGAUGCGGCAUCCGUUGUGUCAUGUCGGCAGACGACUCGCCCGA 2558. .H32
ATCACTTC G CACCTTGTCCC GGGAGGACGAUGCGGAUCACUUCGCACCUUGUCCCCAGACGACUCGCCGGA 2559. .H32
AGTTAGCATCATCTCGTCCG GG6AGGACGAIJGCGGAGUUAGCAUCAUCUCGUCCGCAGACGACUCGCCCGA 2S60. _H32
TGAGATGTCTGTTTGCCGGC GGGAGGACGAUGCGGUGAGAU UCUGUU UGCC GCCAGACGACUCGCCCGA 2561_ .H32
GATCCGGAATCCGTCGTGTA GGGAGGACGAUGCGGGAUCCGGAAUCCGUCGUG UACAGACGACUCGCCCGA 2562. .H32
TCCCGTTCTCTCTCGTACGC GGGAGGACGAUGCGGUCCCGUUCUCUCUCGUACGCCAGACGACUCGCCCGA 2563. H32
T ACCG CGTTCATATCCCG G C GGGAGGACGAUGCGGUACCGCGUUCAUAUCCCGGCCAGACGACUCGCCCGA 2564. _H32
TCGGTGTCCACAG-TTGTGTA GGGAGGACGAUGCGGUCGGUGUCCACAGUUGUGUACAGACGACUCGCCCGA 2565. _H32
TGTG GTGAGTAG GCACTGAC GGGAGGACGAUGCGGUGUGGUGAGUAGGCACUGACCAGACGAC UCGCCCGA 2566. _H32
TTTGTGCAATATTCGTCGTG GGGAGGACGAUGCGGUUUG UGCAAUAUUCGUCGUGCAGACGACUCGCCCGA 2567. H32
TGAGATCTGACGACATGCGC GG6AGGACGAUGCGGUGAGAUCUGACGACAUGCGCCAGACGACUCGCCCGA 2568. H32
ACGATGTACTGGTTTGCCGC GGGAGGACGAUGCGGACGAUGUACUGGUUUGCCGCCAGACGACUCGCCCGA 2569. JH32
ATTG CG TAACC AG CGTTGTA GGGAGGACGAUGCGGAUUGCeUAACCAGCGUUG UACAGACGACUCGCCCGA 2570. „H32
CMTTCGTCTTACGCCACCC GGGAGGACGAUGCGGCAAUUCGUCUUACGCCACCCCAGACGACUCGCCCGA 2571_ ,H32
TCAGAGTACTTGAGAGCCCC GGGAGGACGAUGCGGUCAGAGUACUUGAGAGCCCCCAGACGACUCGCCCGA 2572. .H32
TGAGTAAGCCTGTGTTTGTC GGGAGGACGAUGCGGUGAGUAAGCCUGUGUUUGUCCAGACGACUCGCCCGA 2573. .H32
GTCGCCAATACTTACGGCCG GGGAGGACGAUGCGGGUCGCCAAvJACUUACGGCCGCAGACGACUCGCCCGA 2574. .H32
GTTTG GTACTG GTTTCCCAC GGGAGGACGAUGCGGGUUUGGUACUGGUUUCCCACCAGACGACUCGCCCGA 2575 H32
if in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AAACGACGGTGACGTTACTG GGGAGGACGAUGCGGAAACGACGGUGACGUUACUGCAGACGACUCGCCCGA 2576_ .H32
CACCGCTTTCGTGGTCTCCC GGGAGGACGAUGCGGCACCGCUUUCGUGGUCUCCCCAGACGACUCGCCCGA 2577. „H32
CATCAGTTTTTTCCCGTGTG GGGAGGACGAUGCGGCAUCA.GUUUU UUCCCGUGUGCAGACGACUCGCCCGA 2578. .H32
GTGTCTCTGGTTGGCGGAC GGGAGGACGAUGC6GGUGUCUCUG6UUGGCGGACCAGACGACUCGCCCGA 2579. „H32
AGCGAATAGGGTCAGGTGTG GGGAGGACGAUGCGGAGCGAAUAGGGUCAGGUGUGCAGACGACUCGCCCGA 2580. H32
TTTCGTTTCTAACCCGTGTG GGGAGGACGAUGCGGUUUCGUUUCUAACCCGUG UGCAGACGACUCGCCCGA 2581, H.32
CAG TTGTAAGGTTAGTTG GC GGGAGGACGAUGCGGCAGUUGUAAGGUUAGUUGGCCAGACGACUCGCCCGA 2532. H32
CCGCCATAATCTTCGCTCCC GGGAGGACGAUGCGGCCGCCAUAAUCUUCGCUCCCCAGACGACUCGCCCGA 2583. .H32
GATGACGTGCTGTTGCGCGC GGGAGGACGAUGCGGGAUGACGUGCUGUUGCGCGCCAGACGACUCGCCCGA 2584. _H 2
CTGTGGTAATATTCTGTCCG GGGAGGACGAUGCGGCUGUGGUAAUAUUCUG UCCGCAGACGACUCGCCCGA 2585. .H32
GCCTTTCGTT GCCCCCCCC GGGAGGACGAUGCGGGCCUUUCG UUCUGCCCCCCCCCAGACGACUCGCCCGA 2586. .H32 5250 H04
AAGGTGTAGAGGTGTTGTGC GGGAGGACGAUGCGGAAGGUGUAGAGGUGUUGUGCCAGACGACUCGCCCGA 2587. H32
TC ACCTG ACGTAATCAGG GC GGGAGGACGAUGCGGUCACCUGACGUAAUCAGGGCCAGACGACUCGCCCGA 2588. H32
CGACTGTACGAGTTCCCCCC GGGAGGACGAUGCGGCGACUGUACGAGUUCCCCCCCAGACGACUCGCCCGA 2589, ,H32
GTATATGCTCCGCGATTGGC GGGAGGACGAUGCGGG UAUAUGCUCCGCGAU-UGGCCAGACGACUCGCCCGA 2590. .H32
ATCGACTGTGCTTTCGGGGC GGGAGGACGAUGCGGAUCGACUGUGCUUUCGGGGCCAGACGACUCGCCCGA 2592. .H32
TCCCGAGATTTGTGAGTGTC GGGAGGACGAUGCGGUCCCGAGAUUUGUGAGUGUCGAGACGACUCGCCCGA 2592. .B32
T6GGTCGTCTCCATGGTGCC GGGAGGACGAUGCGGUGGG UCGUCUCCAUGGUGCCCAGACGACUCGCCCGA 2593. .H32
ACGATCGTCGTCTACAGCTG GGGAGGACGAUGCGGACGAUCGUCGUCUACAGCUGCAGACGACUCGCCCGA 2594. .H32
ATCTTCTG G CTTATCGTCCC GGGAGGACGAUGCGGAUeUUCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 2595, _H32
TCCCGCTTTTCGTTGCACCC GGGAGGACGAUGCGGUCCCGCUUUUCGUUGCACCCCAGACGACUCGCCGGA 2595. .H32
AAT AACCTCCATACTG TGTG GGGAGGACGAUGCGGAA.UAACCUCCAUACUGUGUGCAGACGACUCGCCCGA 2597. _H32
AACGTAAACCTCCATACGTC GGGAGGACGAUGCGGAACGUAAACCUCCAUACGUCCAGACGACUCGCCCGA 2598. H32
GTTCGTAGTGTGCAGGGCTG GGGAGGACGAUGCGGGliUCG UAG UGUGCAGGGCUGCAGACGACUCGCCCGA 2599. H32
TG AGTTCCTTCGG TCTGCGC GGGAGGACGAUGCGGUGAGUUCCUUCGGUCUGCGCCAGACGACUCGCCCGA 2600. H32
TCAGGGGATGGCG ATG ATG C GGGAGGACGAUGCGGUCAGGGGAUGGCGAUGAUGCCAGACGACUCGCCCGA 2601. H32
ACGTTGAGTGTGGCGATCGC GGGAGGACGAUGCGGACGUUGAG UGUGGCGAUCGCCAGACGACUCGCCCGA 2602. .H32
AATGATAGGATACACCGCTC GGGAGGACGAUGCGGAAUGAUAGGAUACACCGCUCCAGACGACUCGCCCGA 2603 H32
# trs H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TATGAGGCGTGCTAGCTGTA GGGAGGACGAUGCGGUAUGAGGCGUGCUAGCUGUACAGACGACUCGCCCGA 2604, _H32
TTTGAAGCGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAAGCGGGAUGGGUCCGCAGACGACUCGCCC6A 2605_ _H32
TGCCCAG GATATACTGGCCT GGGAGGACGAUGCGGUGCCCAGGAUAUACUGGCCUCAGACGACUCGCCCGA 2606. _H32
TCATCTGCGTTCGCCCTG GGGAGGACGAUGCGGUCAUCUGCGUUCGCCCUGCAGACGACUCGCCCGA 2507. _H32
CTCCGTACTCCCGAACGCCA GGGAGGACGAUGCGGCUCCG UACUCCCGAACGCCACAGACGACUCGCCCGA 2608. . H32
GTAGGAGGAGGAGGTACCG GGGAGGACGAUGCGGGUAGGAGGAGGAGGUACCGCAGACGACUCGCCCGA 26G9_ _H32
TTTGAAGGAGGGTGGGACTG GGGAGGACGAUGCGG'J UUGAAGGAGGGUGGGACUGCAGACGACUCGCCCGA 2610. .H32
ATACGTGAGACATATGCCTG GGGAGGACGAUGCGGAUACG UGAGACAUAUGCCUGCAGACGACUCGCCCGA 2611. .H32
CACC G TCCTTCG ACAC GTAC GGGAGGACGAUGCGGCACCGUCCU UCGACACGUACCAGACGACUCGCCCGA 2612. _H32
TGCTAACATCTACACATCTG GG6AGGACGAUGCGGUGCUAACAUCUACACAUCUGCAGACGACUCGCCCGA 2613. _H32
CTTCCTGTCGTCTTGTACTG GGGAGGACGAUGCGGCUUCCUGUCGUCUUGUACUGCAGACGACUCGCCCGA 2614. .H32
TGTCGATTCTGGATAG6GCC GGGAGGACGAUGCGGUGUCGAUUCUGGAUAGGGCCCAGAGGACUCGCCCGA 2615. _H32
TTGG TCATTTGTACACACTA GGGAGGACGAUGCGGUUGGUCAUU UGUACACAC JACAGACGA.CUCGCCCGA 2616 mi
CATTTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGCAUUUCCUCUGGUUCGUCCCCAGACGACUCGCCCGA 2617. _H32
TGTCCTTCGTGTGCACTCCC GGGAGGACGAUGCGGUGUCCUUCGUGUGCACUCCCCAGACGACUCGCCCGA 2618. .H32
TATACG GCG GGGTGGGG ATA GGGAGGACGAUGCGGUAUACGGCGGGGUGGGGAUACAGACGACUCGCCCGA 2619. _H32
CGAAGAAGTGTCGTCACGGC GGGAGGACGAUGCGGCGAAGAAG UGUC6UCACGGCCAGACGACUCGCCCGA 2620. _H32
TGTG TTCCTAG TCTGTG GTG GGGAGGACGAUGCGGUGUGUUCCUAGUCUGUGGUGCAGACGACUCGCCCGA 2621. „H32
CCATAACAGGATATCCTCCC GGGAGGACGAUGCGGCCAUAACAGGAUAUCCUCCCCAGACGACUCGCCCGA 2622. _H32
TACCGTTTATG CTCCATGTG GGGAGGACGAUGC6GUACCG UUUAUGCUCCAUGUGCAGACGACUCGCCCGA 2623. _H32
TAGGGTCGATGCTATCTGTG G6GAGGACGAUGCGGUAGGGUCGAUGCUAUCUGUGCAGACGACUCGCCCGA 2624. .H32
TCTGTTAACGTTCACCCCCC GGGAGGACGAUGCGGUCUGUUAACG UUCACCCCCCCAGACGACUCGCCCGA 2625. .H32
TGAGTCACTAGCTGTACGCC GGGA6GACGAUGCGGUGAG UCACUAGCUGUACGCCCAGACGACUCGCCCGA 2626. _H32
TTTGAAGGGTAATGGGACCG GGGA6GACGAUGCGG UUUGAAGGG UAAUGGGACCGCAGACGACUCGCCCGA 2627. _H32
ATAG G G AG AATG GTG AACCG GGGAGGACGAUGCGGAUAGGGAGAAUGGUGAACCGCAGACGACUCGCCCGA 2628, _H32
TTTTGACGTCGGTGTGGCCC GGGAGGACGAUGCGGUUUUGACGUCGG UGUGGCCCCAGACGACUCGCCCGA 2629, _H32
TTTG AAGTAG G ATG GG ACCG GGGAGGACGAUGCGGUUUGAAGUAGGAUGGGACCGCAGACGACUCGCCC6A 2630. _H32
CTACGG TTTGTTTACG CGTA GGGAGGACGAUGCGGCUACGGUUUGUUUACGCGUACAGACGACUCGCCCGA 2631 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTGAATGTAGGGTGGTACCG GGGAGGACGAUGCGGU UGAAUGUAGGGUGG UACCGCAGACGACUCGCCCGA 2632. _H32
CG A ACTG TTTG GTTG CTGTC GGGAGGACGAUGCGGCGAACUG UUUGGUUGCUGUCCAGACGACUCGCCCGA 2633. _H32
GTTGCGCCTGCATTGGTGTG GGGAGGACGAUGC6GGUUGCGCCUGCAUUGG UG UGC.AGACGACUCGCCCGA 2634. ,H32
TCCGGTCGTTACTGTGATGC GGGAGGACGAUGCGGUCCGG UCGUUACUG UGAJGCCAGACGACUCGCCCGA 2635.. .H32
TGAGGCAGACCAfiT GTGIG GGGAGGACGAUGCGGUGAGG'CAGACCAAUUGUGUGCAGACGACUCGCCCGA 2636. .H32
CAACTTTGACCTCTGCTCCC GGGAGGACGAUGCGGCAACUUUGACCUCUGCUCCCCAGACGACUCGCCCGA 2637. _H32
AOTACTCCTCCACCGTCTG GGGAGGACGAUGCGGACUUACUCCUCCACCGUCUGCAGACGACUCGCCCGA 2638. .H32
GGTGTCCGAACTGCTGTCTG GGGAGGACGAUGCGGGGUGUCCGAACUGCUG UCUGCAGACGACUCGCCCGA 2639. ,H32
CGAAGAGTGTTTGTGTACTG GGGAGGACGAUGCGGCGAA.GAG UGUUUG UGUACUGCAGACGACUCGCCCGA 2640_ .H32
TCGTCTTACTAGCCTCCCTG GGGAGGACGAUGCGGUCGUCUUACUAGCCUCCCUG'CAGACGACUCGCCCGA 2641. .H32
ATC GTCTTTCCC CGTTG CCG GGGAGGACGAUGCGGAUCGUCUUUCCCCGUUGCCGCAGACGACUCGCCGGA 2642. _H32
GTCTTCTGGCTCATCGTCCC GGGAGGACGAUGCGGGUCU UC'JGGCUCAUCGUCCCCAGACGACUCGCCCGA 2643. _H32 4980 H04
GAGGTTGTTTGACCGGGCGC GGGAGGACGAUGCGGGAGGUUGUUUGACCGGGCGCCAGACGACUCGCCCGA 2644. H32
TTCACGTGTCCTGCGTCCC GGGAGGACGAUGCGGUUCACGUGUCGUGCGUCCCCAGACGACUCGCCCGA 2645. .H32
AATG G ACTC ACCG CAG CCCG GGGAGGACGAUGCGGAAU6GACUCACCGCAGCCCGCAGACGACUCGCCCGA 2646. .H32
CCCACATTTTGAGGACCCTG GGGAGGACGAUGCGGCCCACAUUUUGAGGACCCUGCAGACGACUCGCCCGA 2647. .H32
ACGTCCGTGTTAGCATGCGC GGGAGGACGAUGCGGACGUCCGUGUUAGCAUGCGCCAGACGACUCGCCCGA 2648. _H32
TCCTTGTCGCCTTGCGTCCC GGGAGGACGAUGCGGUCCUUGUCGCCUUGCGUCCCCAGACGACUCGCCCGA 2649. .H32
AACACTACTCAACGCTTTGC GGGAGGACGAUGCGGAACACUACUCAACGCU UUGCCAGACGACUCGCCCGA 2650. H32
CAACATGGTGAGTCGCTCCC GGGAGGACGAUGCGGCAACAUGGUGAGUCGCUCCCCAGACGACUCGCCCGA 2651. _H32
CACGTTCAATCGTGCGTGGC GGGAGGACGAUGCGGCACGU UCAAUCGUGCGUGGCCAGACGACUCGCCCGA 2652. _H32
TGAGCGGTTCGTCTGTGT'C GGGAGGACGAUGCGGUGAGCGGUUCGUCUGUGUCCAGACGACUCGCCCGA 2653. .H32
TGGCTGACGTCGTCTATGTG GGGAGGACGAUGCGGUGGCUGACGUCGUCUAUGUGCAGACGACUCGCCCGA 2654. .H32
TCGTTGCCCAGTTCTGTGCC GGGAGGACGAUGCGGUCGU UGCCCAGUUCUGUGCCCAGACGACUCGCCCGA 2655. H32
GAAAAGG CGAC AATCTG CTG GGGAGGACGAUGCGGGAAAAGGCGACAAUCUGCUGCAGACGACUCGCCCGA 2656. H32
AAACGGTCCTGCTCCTTCCC G6GAGGACGAUGCGGAAACGGUCCUGCUCCU UCCCCAGACGACUCGCCCGA 2557. .H32
AAAACACACGTTAGG CGCT6 GGGAGGACGAUGCGGAAAACACACGUUAGGCGCUGCAGACGACUCGCCCGA 2658. _H32
TCCTGCATCAAGTCTTGCCG G6GAGGACGAUGCGGUCCUGCAUCAAGUCUUGCCGCAGACGACUCGCCCGA 265 H32
# irt H4
HTS read variable region SNA sequences # in H3 selection selection Selected sequenc
TGCTTGGGCAAGACCGTGTG GGGAGGACGAUGCGGUGCUUGGGCAAGACCGUGUGCAGACGACUCGCCCGA 2660_ ,H32
ACAATCAAATCCTGCCGCCC GGGAGGACGAUGCGGACAAUCAAAUCCUGCCGCCCCA6ACGACUCGCCCGA 2661. .H32
ATCGTTTGTCCGTGTTTGTG GGGAGGACGAUGCGGAUCGUUUGUCCGUGUU DGUGCAGACGACUCGCCCGA 2662_ .H32
CGTTTCCCCT6GTTCGTCCC GGGAGGACGAUGCGGCGUUUCCCCUGGUUCGUCCCCAGACGACUCGCCCGA 2663. H32
TAATACA.CCGTGCTGCTCCC G6GA6GACGAUGCGGUAAUACACCGUGCUGCUCCCCAGACGACUCGCCCGA 2664_ H32
TTGGGTGTTGGGTGGTACTG GGGAGGACGAUGCGGU UGGGUGUUGGGUGGUACUGCAGACGACUCGCCCGA 2665, H32
TTTGAA6GGTGATGATTCCG GGGAGGACGAUGCGGU UUGAAGGGUGAUGAULCCGCAGACGACUCGCCCGA 2666_ H32
ATCCATG AC AGCG CTGTG TG GGGAGGACGAUGCGGAUCCAUGACAGCGCUGUG UGCAGACGACUCGCCCGA 2667_ H32
CCTTTG ACCG G ICG TCTG'G C GGGAG6ACGAUGC6GCCUUUGACCGGUCGUCUGGCCAGACGACUCGCCCGA 2668. .H32
AATG G 6CTCGCTT6TCACTG GGGAGGACGAUGCGGAAUGGGCUCGCUUGUCACUGCAGACGACUCGCCCGA 2669. H32
AATATG TACGTCCCCCTGG C GGGAGGACGAUGCGGAAUAUGUACG UCCCCCUGGCCAGACGACUCGCCCGA 2670_ _H32
TAGTACGCAGTTACCCGGAC GGGAGGACGAUGCGGUAGUACGCAGUUACCCGGACCAGACGACUCGCCCGA 2671. H32
ATATCATGTTTTCACGTGTA GGGAGGACGAUGCGGAUAUCAUGUUUUCACGUGUACAGACGACUCGCCCGA 2672_ H32
TGAGAGTAGGTTATCCGGAC GGGAGGACGAUGCGGUGAGAGUAGGU UAUCCGGACCAGACGACUCGCCCGA 2673_ _H32
TTTGAAGAGTGCTGGGTCCG GGGAGGACGAUGCGGUUUGAAGAGUGCUGGGUCC6CAGACGACUCGCCCGA 2674. _H32
ATACTTGTCTACCCCGTGCC GGGAGGACGAUGCGGAUACU UGUCUACCCCGUGCCCAGACGACUCGCCCGA 267S_ ,H32
TTTGAGGGGTGATGGGCCCG GGGAGGACGAUGCGGUUUGAGGGGUGAUGGGCCCGCAGACGACUCGCCCGA 2676_ .H32 5038 Ή04
ACCGTTGTG G TA AACTG CAC GGGAGGACGAUGCGGACCGUUG UGGUAAACUGCACCAGACGACUCGCCCGA 2677_ .H32
TCCGTCATTCGTCATTGCCC GGGAGGACGAUGCGGUCCGUCAU UCGUCAUUGCCCCAGACGACUCGCCCGA 2678. H32.
GTAC A AACA C CATG G GCTG GGGAGGACGAUGCGGGUACAAACACCAU6AGGCUGCAGACGACUCGCCCGA 2679_ _H32
TGTGATCTGTCGTCGCGTA GGGAGGACGAUGCGGUGUGAUCUG UCGUCGCGUACAGACGACUCGCCCGA 2680_ _H32
CCG ICC ACAGTCTACTG TG GGGAGGACGAUGCGGCCGUCCACAGUCUACUGUGCAGACGACUCGCCCGA 2681. .H32
CATCTGACTCCCCAGCGTAC GGGAGGACGAUGCGGCAUCUGACUCCCCAGCGUACCAGACGACUCGCCCGA 2682. .H32
C AG TAGTG GTCTTG CGTCC C GGGAGGACGAUGCGGCAGUAGUGGUCUUGCGUCCCCAGACGACUCGCCCGA 2683. .H32
GTTG AAGG GTG ATGTGTCCG GGGAGGACGAUGC6GGUUGAAGGG UGAUGU6UCCGCAGACGAC UCGCCCGA 2684. .H32 5098 H04
TTCGAGAGACTTATCCGTGT GGGAGGACGAUGCGGUUCGAGAGACUUAUCCGUGUCAGACGACUCGCCCGA 2685. ^H32
CCGCTTACGAAAATGATCTG GGGAGGACGAUGCGGCCGCUUACGAAAAUGAUCUGCAGACGACUCGCCCGA 2686. .H32
TGTGCCTGTCTGTACACGTA GGGAGGACGAUGCGGUGUGCCUGUCUGUACAC6 JACAGACGACUCGCCCGA 2687 H32
# in H4
HTS read variable region R A sequences # in H3 selection selection Selected sequenc
CTAGGATATAAACTGATCTG GGGAGCACCAUGCGGCUACGAUAUAAACUGAUCUGCAGACGACUCGCCCGA 2688. .H32
ACTCCTCTTCGATGTGGGTA GGGAGGACGAUGCGGACUCCUCUUCGAUGUGG6UACAGACGACUCGCCCGA 2689, „H32
TTGGAAGGGGGGTGGGTCCG GGGAGGACGAUGCGGUUG6AA6GGGGG UGGGUCCGCAGACG ACUCGCCCGA 2690. _H32
CATTCGCAGTCCGTTGGCCC GGGAGGACGAUGCGGCAUUCGCAGUCCGUUGGCCCCAGACGACUCGCCCGA 2691. _H32
TGTGAGCTCATTCCGTTGC GGGAGGACGAUGCGGUGUGAGCUCAUUCCGUUGCCAGACGACUCGC CGA 2692. _H32
TTTTACTCCTGCCTCCCCCC GGGAGGACGAUGCGGU UUUACUCCUGCCUCCCCCCCAGACGACUCGCCCGA 2693. .H32
TCCGTGAATTTCCTGCACCC GGGAGGACGAUGCGGUCCG UGAAUUUCCUGCACCCCAGACGACUCCCCCGA 2694. .H32
TCGTTCAAGTGACCATGCCC GGGAGGACGAUGCGGUCGUUCAAGUGACCAUGCCCCAGACGACUCGCCCGA 2695. .H32
TTTGGTGAGGGGTGGGACTG GGGAGGACGAUGCGGU UUGGUGAGGGGUGGGACUGCAGACGACUCGCCCGA 2696_ .H32
CAGAAGTCCCGATTTGCCGT GGGAGGACGAUG CG GCAGAAG U CCCGAUUUGCCG UCAG ACG ACUCGCCCGA 2697. .H32
GTGTTTCGTCTACCAGTGCC GGGAGGACGAUGCGGGUGUUUCGUCUACCAGUGCCCAGACGACUCGCCCGA 2698_ _H32
TTTGG AGTG GG ATGG GACCG GGGAGGACGAUGCGGU UUGGAG UGGGAUGGGACCGCAGACG ACUCGCCCGA 2699_ _H32
AAGCAACAACTAGCCGCGTC GGGAGGACGAUGCGGAAGCAACAACUAGCCGCGUCCAGACGACUCGCCCGA 2700_ .H32
TGTCGATGGTTG AGCTGGC G6GAGGACGAUGCGGUGUCGAUGG UUGCAGCUGGCCAGACGACUCGCCCGA 2701. .H32
TGGGACATAGCTTCCTTGTG GGGAGGACGAUGCGGUGGGACAUAGCUUCCUUGUGCAG ACG ACUCGCCCGA 2702_ _H32
TTTGGGGTAGGGTG GTACCG GGGA6GACGAUGCGGUUUGGGGUAGGGUGGUACCGCAGACGACUCGCCCGA 2703. _H32
TAG GCG CCGTCTTATGTGTG G6GAGGACGAUGCGGUAGGCGCCGUCUUAUGUGUGCAGACGACUCGCCCGA 2704. „H32
CTATTGGTGAAGGGAGGTG GGGAGGACGAUGCGG'CUAU UGGUGAAGGGAGGUGCAGACGACUCGCCCGA 2705. .H32
GATGACGGCGACTCGGTAAC GGGAGGACGAUGCGGGAUGACGGCGACUCGGUAACCAGACG ACUCGCCCGA 2706. .H32
TCTGAATGGTGAT6GGTCC6 GGGAGGACGAUGCGGUCUGAAUGGUGAU6GG UCCGCA6ACG ACUCGCCCGA 2707. .H32
GTTGAATGGTGATGGGTCCG GGGAGGACGAUGCGGGUUGAAUGGUGAUGGGUCCGCAGACGACUCGCCCGA 2708. .H32
TTTG AAGG ATGCTG GGTCCG GGGAGGACGAUGCGGUUUGAAGGAUGCUGGG UCCGCAGACG ACUCGCCCGA 2709. .H32
AAGGTGTGTCGTGCTGTGTC GGGAGGACGAUGCGGAAGG UGUGUCGUGCUG UGUCCAGACGACUCGCCCGA 2710. .H32
ACATAC ACTTGT GTCCCGT A GGGAGGACGAUGCGGACAUACACUUGUG UCCCG UACAGACGACUCGCCCGA 2711. _H32
TTTGGAGGGGGAAGGGACTG GGGAGGACGAUGCGGU UUGGAGGGGGAAGG6ACUGCAG ACG ACUCGCCCGA 2712. ,H32
CGAATCGTACTCGTGTTCTG GGGAGGACGAUGCGGCGAAUCGUACUCGUGUUCUGCAGACGACUCGCCCGA 2713. .H32
TTTG AAGGG GG ATG CGTCCG GGGAGGACGAUGCGGU UU6AAGGGGGAUGCGUCCGCAGACGACUCGCCCGA 2714. H32
TTTTGAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUUUGAGGGUGAUGGGUCCGCAGACG ACUCGCCCGA 2715. _H32 4537 H04
# in H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc
TGTAGGACGAGGAGGTACCG GGGAGGACGAUGCGGUGUAGGACGAGGAGGUACCGCAGACGACUCGCCCGA 2716. .H32
ICG GTTG CTG CTTCTGTCCC GGGAGGACGAUGCGGUCGG UUGCUGCUUCUGUCCCCAGACGACUCGCCCGA 2717_ .H32
ATACACTCAAACCAGTGCTC GGGAGGACGAUGCGGAUACACUCAAACCAGUGCUCCAGACGACUCG CCCGA 2718, .H32
TCCATCAGAGTCTGTTCCCC GGGAGGACGAUGCGGUCCAUCAGAGUCUGUUCCCCCAGACGACUCGCCCGA 2719, H32
TTTGAAAGGGGATGGGTCCG GGGAGGACGAUGCGGUUUGAAAGGGGAUGGGUCCGCAGACGACUCGCCCGA 2720. .H32
TCCTCGTTGCATACG CACTG GGGAGGACGAUGCGGUCCUCGU UGCAUACGCACUGCAGACGACUCGCCCGA 2721. .H32
TTTGAAGTGTGTTGGGTCCG GGGAGGACGAUGCGGU UUGAAGUGUGUUGGGUCCGCAGACGACUCGCCCGA 2722_ .H32
TAAACTGTG ACAG TTGGTGG GGGAGGACGAUGCGGUAAACUG UGACAGUUGG UGGCAGACGACUCGCCCGA 2723. .H32
TTTGAAGGGTGATGGAACCG GGGAGGACGAUGCGGU UUGAAGGGUGAUGGAACCGCAGACGACUCGCCCGA 2724. .H32
CTGGGTGTAGGGGGGTACCG GGGAGGACGAUGCGGCUGGGUGUAGGGGGG UACCGCAGACGACUCGCCCGA 2725.. „H32
TTGGGTGCAGTGTGGTACCG GGGAGGACGAUGCGGU UGGGUGCA6UGUGGUACCGCAGACGACUCGCCC6A 2726_ _H32
TTGGGAGTAGGGTGGTTCCG GGGAGGACGAUGCGGU UGGGAGUAGGG UGG UUCCGCAGACGACUCGCCCGA 2727. H32
AATCGAACGGGCACATTGCC GGGAGGACGAUGCGGAAUCGAACGGGCACAU UGCCCAGACGACUCGCCCGA 2728. ^H32
ACATCAAGATTGTGTACCGC G6GAGGACGAUGCGGACAUCAAGAUUG UGUACCGCCAGACGACUCGCCCGA 2729. _H32
TGG GTG AGCATG TCATGGG C GGGAGGACGAUGCGGUGGGUGAGCAUGUCAUGGGCCAGACGACUCGCCCGA 2730. .H32
CGATTCCTCTG CTTCG TCCC GG6AGGACGAUGCGGCGAUUCCUCUGCUUCGUCCCCAGACGACUCGCCCGA 2731. _H32
TATT6GAGGTGTAGCGACCG GGGAGGACGAUGCGGUAUUGGAGGUGUAGCGACC6CAGACGACUCGCCCGA 2732. _H32
TCAATGTCC ACCCG CGTCAC G G GAGGACGA UGCGGUCAAUGU CCACCCG CG U CACCAG ACQ AC UCGCCCGA 2733. _H32
TGGTCTTCGATTCCTCCCCC GGGAGGACGAUGCGGUGGUCUUCGAUUCCUCCCCCCAGACGACUCGCCCGA 2734. .H32
TTTGAAG6GGGATGGGTACG GGGAGGACGAUGCGGUUiJGAAGGGGGAUGGGUACGCAGACGACUCGCCCGA 2735. J-B2.
TTTTAAGGG G GATGG GTCCG GGGAGGACGAUGCGGUUUUAA6GGGGAUGGGUCCGCAGACGACUCGCCCGA 273S. H 2 4489 H04
GTATGTCCACTGGGTTGGCC GGGAGGACGAUGCGGGUAUGUCCACUGGGUUGGCCCAGACGACUCGCCCGA 2737. .H32
TATTGGAGGTTTAGGGACCG GGGAGGACGAUGCGGUAUUGGAGGUUUAGGGACCGCAGACGACUCGCCCGA 2738. _H32
TTTCCTTCCCTGAACTGGC GGGAGGACGAUGCGGUUUCCUUCCCUGAACUGGCCAGACGACUCGCCCGA 2739. _H32
CTTGTCATCTTG TTCGTCC C GGGAGGACGAUGCGGCUUG UCAUCUUGUUCGUCCCCAGACGACUCGCCCGA 2740. H32
AT G ATCGTG CACCCTGTG GT GGGAGGACGA'JGCGGAUGAUCGUGCACCCUGUGGUCAGACGACUCGCCCGA 2741_ .H32
AGCCGCACAAGTATTGTGGC GGGAGGACGAUGCGGAGCCGCACAAGUAUUGUGGCCAGACGACUCGCCCGA 2742. ,H32
AATGGCCCATAACGTATCTG GGGAGGACGAUGCGGAAUGGCCCAUAACGUAUCUGCAGACGACUCGCCC6A 2743 H32
» in H4
HTS read variable region NA sequences in H3 selection selection Selected sequenc
CATCAACCTCCGTCCTACCC GGGAGGACGAUGCGGCAUCAACCUCCGUCCUACCCCAGACGACUCGCCCGA 2744_ .H32
ATTGGTGAATG6AGGGACTG GGGAGGACGAUGCGGAUUGGUGAAUGGAGGGACUGCAGACGACUCGCCCGA 2745_ .H32
TCATTCATCATGTTTAGCTG GGGAGGACGAUGCGGUCAUUCAUCAUGUUUAGCUGCAGACGACUCGCCCGA 2746_ _H32
ATC GGTG A AG G GAG G CACTG GGGAGGACGAUGCGGAUCGGUGAAGGGAGGCACUGCAGACGACUCGCCCGA 2747„ _H32
TTTGAAGGGTGAATGGTCC6 GGGAGGACGAUGCGGU UUGAAGGGUGAAUGGUCCGCAGACGACUCGCCCGA 2748_ .H32
AAG77CCTCGTG TCGTTGAC GGGAGGACGAUGCGGAAGUUCCUCGUGUCGUUGACCAGACGACUCGCCCGA 2749.. .H32
ACAATACACTGTACCCGCCC GGGAGGACGAUGCGGACAAUACACUGUACCCGCCCCAGACGACUCGCCCGA 2750„ H32
GATCCATCAATGGTTTGCGC GGGAGGACGAUGCGGGAUCCAUCAAUGGUUUGCGCCAGACGACUCGCCCGA 2751_ .H32
TCTTTCCTCTTGTCGTGCAC GGGAGGACGAUGCGGUCUUUCCUCU UGUCGUGCACCAGACGACUCGCCCGA 2752_ _H32
ATTGGTGGAGGGGGGTACTG GGGAGGACGAUGCGGAUUGGUGGAGGGGGGUACUGCAGACGACUCGCCCGA 2753_ H32
TTTGAAGGGTTVUTGGTCCG G6GAGGACGAUGCGGUUUGAAGGGUUAUUGGUCCGCAGACGACUCGCCCGA 2754_ H32
AAAGAAAGTCACACAATCTG GGGA6GACGAUGCGGAAAGAAAGUCACACAAUCUGCAGACGACUCGCCCGA 275S_ _H32
ACACAACTCTTGGAGTGGCC GGGAGGACGAUGCGGACACAACUCUUGGAG UGGCCCAGACGACUCGCCCGA 2756^ _H32
GTCCTTCTGTCGTATGGTA GGGAGGACGAUGCGGGUCCUUCUGUCGUAUGGUACAGACGACUCGCCCGA 2757_ „H32
TTTGTAGGGTGATGGGTCCC GGGAGGACGAUGCGGUUUGUAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 275S. _H32 3051 H04
TTTGAAGTGTGATGGGACCG GGGAGGACGAUGCGGU UUGAAGUGUGAUGGGACC6CAGACGACUCGCCCGA 2759_ _H32
TTCATGGACTATAGCACCCC GGGAGGACGAUGCGGU UCAUGGACUAUAGCACCCCCAGACGACUCGCCCGA 2760_ .H32
CCG C ATG ATCTACCTCTG C A GGGAGGAGGAUGCGGCCGCAUGAUCUACCUCUGCACAGACGACUCGCCCGA 2761_ .H32
TCG G 77 C GTTCTGTC ATCCC GGGAGGACGAUGCGGUCGGUUCGUUCUGUCAUCCCCAGACGACUCGCCCGA 2762„ .H32
TCTTTGCCTCCCTTGGTTGC GGGAGGACGAUGCGGUCUUUGCCUCCCUUGGUUGCCAGACGACUCGCCCGA 2763_ _H32
GGCGAAGTTTATAACGGCTG GGGAGGACGAUGCGGGGCGAAGUUUAUAACGGCUGCAGACGACUCGCCCGA 2764_ .H32
TTTGAAGGGTGATGGGACGG GGGAGGACGAUGCGGUUUGAAGGGUGAUGG6ACGGCAGACGACUCGCCCGA 276S_ .H32
GTGTCTGATTTGCCTCCCCC GGGAGGACGAUGCGGGUGUCUGAU UUGCCLJCCCCCCAGACGACUCGCCCGA 2766_ .H32
TTGGGAGTCGGGTGGTACCG GGGAGGACGAUGCGGU UGGGAGUCGGGUGG UACCGCAGACGACUCGCCCGA 2767_ .H32
TTTTGAAGGGTGATGGGTCCA GGGAGGACGAUGCGGUUUUGAAGGGUGAUGGGUCCACAGACGACUCGCCCGA 2768, _H32 4737 H04
TA TCCT6ATCGTCAGTGCC GGGAGGACGAUGCGGUACUCCUGAUCGUCAGUGCCCAGACGACUCGCCCGA 2769_ _H32
TCACGGATGGAGGTGATGTG GGGAGGACGAUGCGGUCACGGAUGGAGGUGAUGUGCAGACGACUCGCCCGA 2770_ H32
GCCTTTCGTTCTGCCTCC GGGAGGACGAUGCGGGCCUU UCGUUCUGCCUCCCAGACGACUCGCCCGA 2771 H32 3173 H04
# m H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TCG GGTG TAG GG TG TTACCG GGGAGGACGAUGCGGUCGGGUGUAGGGUGUUACCGCAGACGACUCGCCCGA 2772. _H32
TTTGAAGGGT ATAG GGCCG GGGAGGACGAUGCGGUU UGAAGGGUGAUAGGGCCGCAGACGACUCGCCCGA 2773, .H32
GAAAAGTGAATAGGCTACCC GGGAGGACGAUGCGGGAAAAGUGAAUAGGCUACCCCAGACGACUCGCCCGA 2774, _H32
TTGGT7TAGGGTGGTACCG GGGAGGACGAUGCGGUUGGUUUAGGG UGGUACCGCAGACGACUCGCCCGA 2775, _H32
ACCGTTTCGTCGCCAAGCAC GGGAGGACGAUGCGGACCGUUUCGUCGCCAAGCACCAGACGACUCGCCCGA 2776_ _H32
AATTCATTTCCTCCCCCCCC GGGAGGACGAUGCGGAAUUCAUUUCCUCCCCCCCCCAGACGACUCGCCCGA 2777. .H32
TTGG GTATAGG GTG GTACTG GGGAGGACGAUGCGG UUGGGUAUAGGGUGGUACUGCAGACGACUCGCCCGA 2778. _H32
TCCAG6CA6AAT G ICACCA GGGAGGACGAUGCGGUCCAGGCAGAAUCGUCACCACAGACGACUCGCCCGA 2779. .H32
TCTTCCGAAGCGTGGTTCTG GGGAGGACGAUGCGGUCUUCCGAAGCGUGGUUCUGCAGACGACUCGCCCGA 2780. .H32
TTTCCCTTTCGACTGCTGCC G6GAGGACGAUGCGGUUUCCCUUUC6ACUGCU6CCCAGACGACUCGCCC6A 2781. .H32
TCCCTCTTTTGTTCGTG GTC GGGAGGACGAUGCGGUCCCUCUUU UGUUCGUGGUCCAGACGACUCGCCCGA 2782. _H32
TTGGAAGGGGGGTGGTACCG GGGAGGACGAUGCGGUUGGAAGGGGGG UGGUACCGCAGACGACUCGCCCGA 2783. _H32
TTCACTCCAA A ACAC ACCGC GGGAGGACGAUGCGGU UCACUCCAAAACACACCGCCAGACGACUCGCCCGA 2784. .H32
TCAGTGTGATAATATTGCGG GGGAGGACGAUGCGGUCAGUG UGAUAAUAUUGCGGCAGACGACUCGCCCGA 2785. „H32
TTT6GTGTATGGTGGTACCG GGGAGGACGAUGCGGU UUGG UGUAUGGUGGUACCGCAGACXiACUCGCCCGA 2786. ^H32
GTTGGTGAA66GAGGGACT6 GGGAGGACGAUGCGGGUUGGUGAAGGGAGGGACUGCAGACGACUCGCCCGA 2787. _H32
CCTGATGTTACTATTCGTGC 6GGAGGACGAUGCGGCCUGAUG UUACUAUUCG UGCCAGACGACUCGCCCGA 2788. .H32
TGACGAGCGTGCGTTTGGTC GGGAGGACGAUGCGGU6ACGAGCGUGCGUUUGGUCCAGACGACUC6CCC6A 278.9. _H32
TTG GGTGTG G G GTG GG ACCG GG6AGGACGAUGCGGUUGGGUGUGGGGU6GGACCGCAGACGACUCGCCCGA 2790. _H32
rrCACCTTTTCGATCCGCCC GGGAGGACGAUGCGGU UCACCUUUUCGAU CCGCCCCAGACGACUCGCCCGA 2791. _H32
TTTGACGGGTGATGGGACCG GGGAGGACGAUGCGGU UUGACGGGUGAUGGGACCGCAGACGACUCGCCCGA 2792._ _H32
ACTAGCATCACGGTTGTCCA GGGAGGACGAUGCGGACUAGCAUCACGGUUGUCCACAGACGACUCGCCCGA 2793. _H32
TTGGGTGTG6TGGTACCG GGGAGGACGAUGCGGU UGGGUGUG6UGGUACCGCAGACGACUCGCCCG A 2794_ .H32
CAGAGGTTGGTCGTCGAGGC GGGAGGACGAUGCGGCAGAGGUUGGUCG UCGAGGCCAGACGACUCGCCCGA 2795. .H32
GTTTTACAAG GTAC AGTCTG GGGAGGACGAUGCGGGUUUUACAAGGUACAGUCUGCAGACGACUCGCCCGA 2796. .H32
TTTGAAGGGTG ATGCCTCCG GGGAGGACGAUGCGG UUGAAGGGUGAUGCCUCCGCAGACGACUCGCCCGA 2797. _H32
TTTG AAG G AGG ATG G G ACTG GGGAGGACGAUGCGGU UUGAAGGAGGAUGGGACUGCAGACGACUCGCCC6A 2798. ,H32
AATCCTCTGTGTTGTCTGGC GGGAGGACGAUGCGGAAUCCUCUGUGUUGUCUGGCCAGACGACUCGCCCGA 2799 H32
# in m
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
GCAGGGAAACTCTTCACGCC GGGAGGACGAUGCGGGCAGGGAAACUCUUGACGCCCAGACGACUCGCCCGA 2800. .H32
TTTGCAGGGTGATGCGTCCG GGGAGGACGAUGCGGUUUGCAGGGUGAUGCG UCCGCAGACGACUCGCCCGA 2801_ J-B2
ICG GGTCGTGCGTGTGTTGC GGGAGGACGAUGCGGUCGG6UCGUGCGUGUGUU6CCAGACGACUCGCCCGA 2802_ _H32
TCCTCATGTCTATGTCTCCG GGGAGGACGAUGCGGUCCUCAUGUCUAUGUCUCCGCAGACGACUCGCCCGA 2803. .H32
TCCAACGTCGATTCTGTTGC GGGAGGACGAUGCGGUCCAACGUCGAU UCUGUUGCCAGACGACUCGCCCGA 2804_ _H32
TCATTCCCTTAG CGACTCCG GGGAGGACGAUGCGGUCAUUCCCUUAGCGACUCCGCAGACGACUCGCCCGA 2805_ _H32
ACAGCCCTATAHX^ACCG GGGAGGACGAUGCGGACAGCCCUAUALtUGUUACCGCAGACGACUCGCCCGA 2806. .H32
TTTGAAGGTTGTTGGGTCC6 GGGAGGACGAUGCGGUUUGAAGGUUGUUGGGUCCGCAGACGACUCGCCCGA 2807. _H32
GCTTATCTTCG TTTGTG CG C GGGAGGACGAUGCGGGCUUAUCUUCGUUUGUGCGCCAGACGACUCGCCCGA 2808. .H32
TCATCCTATGTAGTGTTGTG GGGAGGACGAUGCGGUCAUCCUAUGUAGUGUUGUGCAGACGACUCGCCCGA 2809. .H32
TCCTTAAGCGTACTGGTGCC GGGAGGACGAUGCGGUCCUUAAGCGUACUGGUGCCCAGACGACUCGCCCGA 2810. „H32
GGATGGTTGTGGCTATACGC GGGAGGACGAUGCGGGGAUGGUUGUGGCUAUACGCCAGACGACUCGCCCGA 2811_ _H32
GTCCGTCTGACTTATCCCTG GGGAGGACGAUGCGGG UCCG UCUGACUUAUCCCUGCAGACGACUCGCCCGA 2812. .H32
TCCTGCGTAAACTCGTGCCG GGGAGGACGAUGCGGUCCUGCGUAAACUCGUGCCGCAGACGACUCGCCCGA 2813_ .H32
TATTG CC ACG G G G CAATAGC GGGAGGACGAUGCGGUAUUGCCACGGGGCAAUAGCCAGACGACUCGCCCGA 2814 .H32
TATCGAAACAGTCTG GTCTG GGGAGGACGAUGCGGUAUCGAAACAGUCUGGUCUGCAGACGACUCGCCCGA 2815,, .H32
TGGGCATGAGATCGTAGGAC GGGAGGACGAUGCGGUGGGCAUGAGAUCGUAGGACCAGACGACUCGCCCGA 2816, H32
GCG A AATG TCG'TG CTCTCCC G6GAGGACGAUGCG6GCGAAAUGUCGUGCUCUCCCCAGACGACUCGCCCGA 2817, H32
TTGGGA.GTAGGGTGGGTCCG GGGAGGACGAUGCGGUUGGGAGUAGGGUGGGUCCGCAGACGACUCGCCCGA 2818. H32
GACCGCGATCCATCTCCGTA GGGAGGACGAUGCGGGACCGCGAUCCAUCUCCGUACAGACGACUCGCCCGA 2S19_ H32
TCAGTTAGTCCCGTCCGTGC GGGAGGACGAUGCGGUCAGU UAGUCCCGUCCGUGCCAGACGACUCGCCCGA 2820_ .H32
AGTGGACTATGATGATGGCC GGGAGGACGAUGCGGAGUGGACUAUGAUGAUGGCCCAGACGACUCGCCCGA 2821_ .H32
TCTTCGAAAACCGAGTTGTG GGGAGGACGAUGCGGUCUUCGAAAACCGAGU UGUGCAGACGACUCGCCCGA 2S22^ H32
AGTGGCCTATACCACTTGGC GGGAGGACGAUGCGGAGUGGCCUAUACCACUUG6CCAGACGACUCGCCCGA 2823_ H32
GAAAG CAC ACTG G ACTTGTG GGGAGGACGAUGCGGGAAAGCACACUGGACU UG UGCAGACGACUCGCCCGA 2824. H32
TGTTGTTAGTCTGATGTTGC GGGAGGACGAUGCGGUG UUGUUAGUCUGAUGUUGCCAGACGACUCGCCCGA 2825. H32
TTAGAGCTTGCATTGGGCAC GGGAGGACGAUGCGGUUAGAGCUUGCAUUGGGCACCAGACGACUCGCCCGA 2826. _H32
CGTTGTCTTCTTCACGTGTC GGGAGGACGAUGCGGCGUUGUCUUCUUCACGUGJCCAGACGACUCGCCCGA 2827 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TG A AAC AA ATCTAT6CTCCC G6GAGGACGAU6CGGUGAAACAAAUCUAUGCUCCCCAGACGACUCGCCCGA 2828. JH32
GTACGTGTGAGCAATGTGGC GGGAGGACGAUGCGGGUACGUGUGAGCAAUGUGGCCAGACGACUCGCCCGA 2829. .H32
ACGATGATCGTCTGTACGGC GGGAGGACGAUGCGGACGAUGAUCGUCUGUACGGCCAGACGACUCGCCCGA 2830. .H32
TCGCAGAAAGG ATG CGTG CC GGGAGGACGAUGCGGUCGCAGAAAGGAUGCGUGCCCAGACGACliCGCCCGA 2831„ ,H32
TACTGAAAACAAACTGTCTG GGGAGGACGAUGCGGUACUGAAAACAAACUGUCUGCAGACGACUCGCCCGA 2832. _H32
T'TGGGCGTATGGTGGTACCG GGGAGGACGAUGCGGUUGGGCGUAUGGUGGUACCGCAGACGACUCGCCCGA 2833. H32
TTTGAAGAGTGATGGGACCG GGGAGGACGAUGCGGUUUGAAGAGUGAUGGGACCGCAGACGACUCGCCCGA 2834. H32 5244 H04
GCCGTTATCTGGTGTGCGTG GGGAGGACGAUGCGGGCCG UUAUCUGGUGUGCGUGCAGACGACUCGCCCGA 2335. H32
TTTGAAGGGTGATGGGTCTA GGGAGGACGAUGCGGU UUGAAGGGUGAUGGGUCUACAGACGACUCGCCCGA 2836_ H32
GCTTCAAAGTCTTCCGTGTG GGGAGGACGAUGCGGGCUUCAAAGUCUUCCGUG UGCAGACGACUCGCCCGA 2837. H32
TCCTGTCGTTTTTGCGTCCC GGGAGGACGAUGCGGUCCUGUCGU UUUUGCGUCCCCAGACGACUCGCCCGA 2838_ .H32 3378 H04
AACTAGCCGAGCGGTATCGC GGGAGGACGAUGCGGAACUAGCCGAGCGGUAUCGCCAGACGACUCGCCCGA 2839_ _H32
TTACAACACGTCCCCATCCC GGGAGGACGAU6CGGUUACAACACGUCCCCAUCCCCAGACGACUCGCCCGA 2840_ H32
TAAG CAACTC CATG ATA CTG GGGAGGACGAUGCGGUAAGCAACUCCAUGAUACU6CAGACGACUCGCCCGA 2841_ _H32
TTTGAGCGGTGATGGGTCCG GGGA6GACGAUGCGGU UUGAGCGG UGAUGGGUCCGCAGACGACUCGCCCGA 2842. .H32
GCCGCGTCTAGCCTT CG TAC GGGAGGACGAUGCGGGCCGCGUCUAGCCUUCGUACCAGACGACUCGCCCGA 2843. ,H32
GTTAG TCCA AATCGCGTG TA GGGAGGACGAUGCGGGUUAGUCCAAAUCGCGUGUACAGACGACUCGCCCGA 2844_ -H32
GACAGTGTTAAAAGGGGCCG GGGAGGACGAUGCGGGACAG UGU UAAAAGGGGCCGCAGACGACUCGCCCGA 2845.. .H32
GCCTTTC6TTCTGCTCTACC GGGAGGACGAUGCGGGCCUUUCGUUCUGCUCUACCCAGACGACUCGCCCGA 2846. .H32
TCGTACGAGTGCATCTGGTG GGGAGGACGAUGCGGUCGUAC6AGUGCAUCUGGUGCAGACGACUCGCCCGA 2847. _M32
GCTTACGTCTCAATCCACCC GGGAGGACGAUGCGGGCUUACGUCUCAAUCCACCCCAGACGACUCGCCCGA 2848. _H32
GTGGGTGTAGGGCGGTACCG GGGAGGACGAUGCGGGUGGGUGUAGGGCGGUACCGCAGACGACUCGCCCGA 2849. „H32
TAAGCAAAACTATACAGCCG GGGAGGACGAUGCGGUAAGCAAAACUAUACAGCCGCAGACGACUCGCCC A 2850. ,H32
AACACTATACCACGGGACTG GGGAGGACGAUGCGGAACACUAUACCACGGGACUGCAGACGACUCGCCCGA 2851. .H32
ACGAGACTGCGGCCTGTGAC GGGAGGACGAUGCGGACGAGACUGCGGCCUGUGACCAGACGACUCGCCCGA 2852. .H32
αποτοΑτατττ6Α6α6το G6GAGGACGAUGCG6GUUGUCAUGUUUGAGCUG UGCAGACGACUCGCC.CGA 2853. _H32
GCATAAC G TACTG G TC ACCC GGGAGGACGAUGCGGGCAUAACGUACUGGUCACCCCAGACGACUCGCCCGA 2854. _H32
TTCCTTTCCTCCCGTTTTGC GGGAGGACGAUGCGGUUCCUUUCCUCCCGU UUUGCCAGACGAC'JCGCCCGA 2855 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTC ATTTAGGTTG G GTG CTG GGGAGGACGAUGCGGU UGAUU UAGGUUGG6UGCUGCAGACGACUCGCCCGA 2856_ .H32
ACGTGGTGTAGGGAGGTGTC GGGAGGACGAUGCGGACGUGG UGUAGGGAGGUGUCCAGACGACUCGCCCGA 2857. _H32
CTTGTCGTCTTGTTCGTGCC GGGAGGACGAUGCGGCUUGUCGUCUUGUUCGUGCCCAGACGACUCGCCCGA 2858_ _H32
ACTAGTAAACCTCCGTGGC GGGAGGACGAUGCGGACUAGUAAACCUCCGUGGCCAGACGACUCGCCCGA 2859. .H32
AGTTTGATGTTGATGGTGTG GGGAGGACGAUGCGGAGUU UGAUGUUGAUGGUGUGCAGACGACUCGCCCGA 2860. _H32
TTTGAAGGGTGATGCGGCCG GGGAGGACGAUGCGGU UUGAAGGG UGAUGCGGCCGCAGACGACUCGCCCGA 2861. .H32
TTGGGTGGAGGGTGGCACCG GGGAGGACGAUGCGGU UGGGUGGAGGGUGGCACCGCAGACGACUCGCCCGA 2862. ,H32
TCTTTGTCGTCTTGCGTCCC GGGAGGACGAUGCGGUCUUUGUCC UCUUGCGUCCCCAGACGACUCGCCCGA 2863. ,H32
TATTCG ATTTC G TCGTGGTC GGGAGGACGAUGCGGUAUUCGAUUUCGUCGUGG UCCAGACGACUCGCCCGA 2864_ .H32
TAATTTCGTCCTAACG TCCC GGGAGGACGAUGCGGUAAUUUCGUCCUAACGUCCCCAGACGACUCGCCC6A 2865,, H32
TTGGGTGTGGGGTGGTTCCG GGGAGGACGAUGCGGUUGGGUGUGGGGUGGUUCCGCAGACGACUCGCCCGA 2866. H32
TTTGAGGG GTGTTGGGTCCG GGGAGGACGAUGCGGU UUGAGGGGUGUUGGGUCCGCAGACGACUCGCCCGA 2867. H32 2958 H04
TAACCGAAATCTGTAGTGTG GGGAGGACGAUGCGGUAACCGAAAUCUGUAGUGUGCAGACGACUCGCCCGA 2868. .H32
ATAAGGAGAAGGGTGAACCG GGGAGGACGAUGCGGAUAAGGAGAAGGGUGAACCGCAGACGACUCGCCCGA 2869. .H32
ATAGGGAGAAGGGTAAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGUAAACCGCAGACGACUCGCCCG 2870_ _H32
TIG G GTG TAG G 6 TG GTAATG GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUAAUGCAGACGACUCGCCCGA 28 1. _H32
GTTCAACAG CCG CCTTTGTG GGGAGGACGAUGCGGGUUCAACAGCCGCCUUUG UGCAGACGACUCGCCCGA 2872. H32
ATAGGGTGAAGGGTGAACCG GGGAGGACGAUGCGGAUAGGG UGAAGGGUGAACCGCAGACGACUCGCCCGA. 2873_ H32
TTTGGGGGGTGATGGGACCG GGGAGGACGAUGCGGU UUGGGGGG UGAUGGGACCGCAGACGACUCGCCCG A 2874. .H32
1 CTTTCGTCCCCTTATGTG GGGAGGACGAU6CGGUUCUUUC6UCCCCUUAUGUGCAGACGACUCGCCC6A 2875. .H32
CAATCAAACTGTCTTCCCCC GGGAGGACGAUGCGGCAAUCAAACUGUCUUCCCGCCAGACGACUCGCCCGA 2876. „H32
ATCCGCTTCTCCCTTG G CAC GGGAGGACGAUGCGGAUCCGCUUCUCCCUUGGCACCAGACGACUCGCCCGA 2877. H32
TGTGCTGTG T ACTATG TG TA GGGAGGACGAUGCGGUGUGCUGUG UACUAUGUGUACAGACGACUCGCCCGA 2878. .H32
TTGGGTGTAGGGTAATACCG GGGAGGACGAUGCGGU UGGGUGUAGGGUAAUACCGCA6ACGACUC6CCCGA 2879. .H32
GAGGCGTGTTCTCTGGTCGC GGGAGGACGAUGCGGGAGGCGUGULJCUCUGGUCGCCAGACGACUCGCCCGA 2880. _H32
TCCAAATATGTCGCGCTCCC GGGAGGACGAUGCGGUCCAAAUAUGUCGCGCUCCCCAGACGACUCGCCCGA 2881. _H32
GTTGAAGG GTGATG GGTCGG GGGAGGACGAUGC6GG UUGAAGGGUGAUGGGUCGGCAGACGACUCGCCCGA 2882. _H32
AAATGG G ACG ATTTCACCAG GGGAGGACGAUGCGGAAAUGGGACGAUUUCACCAGCAGACGACUCGCCCGA 2883 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTCG CTG TTCATACCC ACCC GGGAGGACGAUGCGGUUCGCUG U UCAUACCCACCCCAGACGACUCGCCCGA 2884. „H32
TACGGGTTATATGCTCTGTG GGGAGGACGAUGCGGUACGGGUUAUAUGCUCUGUGCAGACGACUCGCCCGA 2885. .H32
TCCG G AC G C ATG TTCGACTG GGGAGGACGAUGCGGUCCGGACGCAUG UUC6ACUGCAGACGACUCGCCCGA 2886_ .H32
GCCGTCATTTAATGTGGCCC GGGAGGACGAUGCGGGCCGUCAU UUAAUGUGGCCCCAGACGACUCGCCCGA 2887. .H32
TGCACTTTGG G ATTG GCCCA GGGAGGACGAUGCGGUGCACUUU6GGAUUGGCCCACAGACGACUCGCCCGA 2888. _H32
TCGTTTCCCATGTGTGTCAC GGGAGGACGAUGC6GUCGUUUCCCAUG UGUGUCACCAGACGACUCGCCCGA 2889_ _H32
CCATTCG CCTGT ATCG AGTA GGGAGGACGAUGCGGCCAUUCGCCUGUAUCGAGUACA.GACGACUCGCCC6A 2890. .H32
GAGCTCGTTCTGTGAC CTG GGGAGGACGAUGCG6GAGCUCGUUCUGUGACGCUGCAGACGACUCGCCCGA 2891. H32
CACTGAACTGATGGCTACCC GGGAGGACGAUGCGGCACUGAACUGAUGGCUACCCCAGACGACUCGCCCGA 2892. _H32
CACTTCATCGAACGACTCCC GGGAGGACGAUGCGGCACUUCAUCGAACGACUCCCCAGACGACUCGCCCGA 2893. H32
CATCAAACGGGGTGAGTGCC GGGAGGACGAUGCGGCAUCAAACGGGGUGAGUGCCCAGACGACUCGCCCGA 2894. H32
AAAGAGCTGAGTCGCTGTGCC GGGAGGACGAUGCGGAAAGAGCUGAGUCGCUGUGCCCAGACGACUCGCCCGA 2895. H 32
TTTGCAGGGTGATGGGTCCC GGGAGGACGAUGCGGUUUGCAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 2896. H32 3571 H04
•J\
TTGG GTCTAGG GTGTTAC C G GGGAGGACGAUGCGGUUGGGUCUAGGGUGUUACCGCAGACGACUCGCCCGA 2897. .H32
CGTGTCGCTCGGTCTGTG GGGAGGACGAU6CGGCGUGUCGCUCGGUCUGUGCAGACGACUCGCCCGA 2898. „H32
TGAATG CTCTTCTGTTG GTA GGGAGGACGAUGCGG UGAAUGCUCUUCUGUUGGUACAGACGACUCGCCCGA 2899. _H32
A AAC ATGTTAC A G CCG CTA GGGAGGACGAUGCGGAAACAUGUUACAAGCCGCLACAGACGACUCGCCCGA 29QD. _H32
6TTGATGTGGGTATTCACCC GGGAGGACGAUGCGGGUUGAUG UGGGUAUUCACCCCAGACGACUCGCCCGA 2901. H32
ACATGCGTACGGACTTGG6C GGGAGGACGAUGCGGACAUGCGUACGGACUUGGGCCAGACGACUCGCCCGA 2902. .H32
ATTGAAGGAGGGAGGGACTG GGGAGGACGAUGCGGAU UGAAGGAGGGAGGGACUGCAGACGACUCGCCCGA 2903. .H32
GTTTAAGGGTGATGGGTCCG GGGAGGACGAUGCGGGUUUAAGGG UGAUGGGUCC6CAGACGACUCGCCCGA 2904. H32
G AAC ATG ACTT CTCTACCCC GGGAGGACGAUGCGGGAACAUGACUUCUCUACCCCCAGACGACUCGCCCGA 2905. .H32
TCATGTCCCCTATGTACCGC GGGAGGACGAUGCGGUCAUGUCCCCUAUGUACCGCCAGACGACUCGCCCGA 2906. _H32
TGGGCCGTAGTGCGTCTGTG GGGAGGACGAUGCGGUGGGCCGUAGUGCGUCUGiJGCAGACGACUCGCCCGA 2907. .H32
A ATA AAGTCCG ACTTG G CTG GGGAGGACGAUGCGGMUAAAGUCCGACUUGGCUGCAGACGACUCGCCCGA 2908. .H32
ACCTATA ATTAGTCTG TGTG GGGAGGACGAUGCGGACCUAUAAUUAGUCUG UGUGCAGACGACUCGCCCGA 2909.. .H32
TTGATGACTTATGGCACATG GGGAGGACGAUGCGG UUGAUGACUUAUGGCACAUGCAGACGACUCGCCCGA 2910. .H32
TGTAGAGAGGTAGTTATCGT GGGAGGACGAUGCGG UGUAGAGAGGUAGU UAUCG UCAGACGACUCGCCCGA 2911 H 2
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATACG GTGTGTG CTTGG CCC GGGAGGACGAUGCGGAUACGGUGUGUGCUUGGCCCCAGACGACUCGCCCGA 2912. H32
TTGGGTGTAGGGTAGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGGGUAGGUACCGCAGACGACUCGCCCGA 2913, .H32
GGTGGTGAAGGGA6GTACTG GGGAGGACGAUGCGGGGUGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 2914. .H32
ATTTAGTCCTCGGGTTGCTG GGGAGGACGAUGCGGAUUUAGUCCUCGGGUUGCUGCAGACGACUCGCCCGA 2915. ,H32
GTAGCACAAGTCTGCTTGGC GGGAGGACGAUGCGGGUAGCACAAGUCUGCUUGGCCAGACGACU.CGCCCGA 2916_ .H32
TTTGAAGAGCGATGGGTCCG GGGAGGACGAUGCGGU UUGAAGAGCGAUGGGUCCGCAGACGACUCGCCCGA 2917_ .H32 4025 H04
TGATCGT'CAGTTCTCCTGTG GGGA6GACGAUGCGGUGAUCGUCAGUUCUCCUGUGCAGACGACUCGCCCGA 2918. _H32
TCGAGGTAGGAATGGCGTGC GGGAGGACGAUGCGGUCGAGGUAGGAAUGGCGUGCCAGACGACUCGCCCGA 2919, _H32
TTGG G TG TAG G G C G G TACCC GGGAGGACGAUGCGGUUGGGUGUAGGGCGGUACCCCAGACGACUCGC CGA 292Q_ .H32
TCACCTAACGGTTACACCAT GGGAGGACGAUGCGGUCACCUAACGGUUACACCAUCAGACGACUCGCCCGA 2921_ _H32
CGGTGAAGGGAGGTACTG GGGAGGACGAUGCGGCGGUGAAGGGAGGUACUGCAGACGACUCGCCCGA 2922_ JH32
TTAGCGTTCTTCCCGGGTGC GGGAGGACGAUGCGGUUAGCGUUCUUCCCGGG UGCCAGACGACUCGCCGGA 2923. .H32
TGATTCGTGTACTCCGGCCC GGGAGGACGAUGCGGUGAU UCGUG UACUCCGGCCCCAGACGACUCGCCCGA 2924, .H32
TTTGAAGGGGGATGGTCCG GGGAGGACGAUGCGGUUUGAAGGGGGAUGGUCCGCAGACGACUCGCCCGA 2925_ .H32
AGAAGAGTTGGTTGTGCCCC GGGAG6ACGAUGCGGAGAAGAGUUGGUUGUGCCCCCAGACGACUCGCCCGA 2926_ .H32
ACACCGTGGTCCCAATTGGC GGGAGGACGAUGCGGACACCGUGGUCCCAAUUGGCCAGACGACUCGCCCGA 2927. _H32
TACGAGCCGACTGCGTACGC GGGAGGACGAUGCGG UACGAGCCGACUGCGUACGCCAGACGACUCGCCCGA 2928. .H32
TGTCCGTTTCTCCCCGTAAC GGGAGGACGAUGCGGUG UCCGUUUCUCCCCGUAACCAGACGACUCGCCCGA 2929„ .H32
TCTGCGGCGTATTGCATCAC GGGAGGACGAUGCGGUCUGCGGCGUAUUGCAUCACCAGACGACUCGCCCGA 2930. .H32
TTCGTFTGGCGATACGTGTC GGGAGGACGA.UGCGGUUCGUUUGGCGAUACGUGUCCAGACGACUCGCCCGA 2931_ „H32
TTGGGTGTAGTGCGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGUGCGGUACCGCAGAC6ACUCGCCCGA 2932. .M32
GATGTCATGGCATGTGTGGC GGGAGGACGAUGCGGGAUGUCAUGGCAUG UGUGGCCAGACGACUCGCCCGA 2933. .H32
CAGACTAGACAATCAGTGTG GGGAGGACGAUGCGGCAGACUAGACAA.UCAGUGUGCAGACGACUCGCCCGA 2934. .H32
TTTG AATTGTG ATGG GTCCG GGGAGGACGAUGCGGU UUGAAUUGUGAUGGGUCCGCAGACGACUCGCCCGA 2935„ .H32
CATGGGA CC ACAACTCACT G GGGAGGACGAUGCGGCAUGGGACCACAACUCACUGCAGACGACUCGCCCGA 2936. _H32
CATGGATTGACGTTGCTGTG GGGAGGACGAUGCGGCAUGGAUUGACGUUGCUGUGCAGACGACUCGCCCGA 2937. „H32
CAGTATGTCTGCATTGGCTG GGGAGGACGAUGCGGCAGUAUGUCUGCAUUGGCUGCAGACGACUCGCCCGA 2938. .H32
TATACG6C6GCGTGGGGATA GGGAGGACGAUGCGGUAUACGGCGGCGUGGGGAUACAGACGACUCGCCCGA 2939 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATGAGGACCATTCACCACAC G 6GAGG ACGA UG CG6A U GAG GACCAU U CACC A CACC AGACG AC U CGCCCG A 9 0_ H32
TGTG CATATTTAACG CTCTG GGGAGGACGAUGCGGUGUGCAUAUUUAACGCUCUGCAGACGACUCGCCCGA 2941_ H32
TTG G CGTAGG GTG GTACCG GGGAGGACGAUGCGGUUGGCGUAGGGUGGUACCGCAGACGACUCGCCCGA 2942_ H 2
ACCG TG ATCATAGTGCGCCC GGGAGGACGAUGCGGACCGUGAUCAUAGUGCGCCCCAGACGACUCGCCCGA 2943_ H32
T6AGTTCTTGTAAGGCTCCT GGGAGGACGAUGCGGUGAGUUCUUGUAAGGCUCCUCAGACGACUCGCCCGA 2944„ H32
TCCGGCTTACTGATCCGCCA GGGAGGACGAUGCGGUCCGGCUUACUGAUCCGCCACAGACGACUCGCCCGA 2945_ H32
CCATCGCTAATAACCCGCGC GGGAGGAC6AUGCGGCCAUCGCUAAUAACCCGCGCCAGACGACUCGCCCGA 2946_ H32
GCATTGTATGTGGGGTGGTC GGGAGGACGAUGCGGGCAUUGUAUGUGGG6UGGUCCAGACGACUCGCCCGA 2947. H32
ATGATCACGTTAAGCCCCTG GGGAGGACGAUGCGGAU6AUCACGUUAAGCCCCUGCAGACGACUCGCCCGA 2948_ H32
TCTTCAACCAAATGTATGCC GGGAGGACGAUGCGGUCUUCAACCAAALJGUAUGCCCAGAC6ACUCGCCCGA 2949. H32
TTTGAA6GGTGATGGGTTGG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGUUGGCAGACGACUCGCCCGA 2950_ .H32
TACTG TTCCG C CCCG TTG TA GGGAGGACGAUGCGGUACUG UUCCGCCCCGU UGUACAGACGACUCGCCCGA 2951_ H32
TCTTACGTCGCTGCAGACCC GGGAGGACGAUGCGGUCUUACGUCGCUGCAGACCCCAGACGACUCGCCCGA 2952_ H32
CCGCGTAATCAAGCCATGTC GGGAGGACGAUGCGGCCGCGUAAUCAAGCCAUGUCCAGACGACUCGCCCGA 2953_ .H32
GGAGTGTGATTCAGCTTGTG GGGAGGACGAUGCGGGGAGUGUGAUUCAGCUUGUGCAGACGACUC.GCCCGA 2954_ H32
GCCTTTCGTTCTTCTCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUCUCUCCCCAGACGACUCGCCCGA 2955. .H32 3392 H04
TATGATCTTGAAATGCTGTG GGGAGGACGAUGCGGUAUGAUCU UGAAAUGCUGUGCAGACGACUCGCCCGA 2956_ H32
AAATCCTCCAACGTATTCCC GGGAGGACGAUGCGGAAAUCCUCCAACGUAUUCCCCAGACGACUCGCCCGA 2957_ H32
ATCTAACCCGCAAGTGCCG GGGAGGACGAUGCGGAUCUAACCCGCAAGUGCCGCAGACGACUCGCCCGA 2958. .H32
CAATCTATAGGGGATGTGTC GGGAGGACGAU6CGGCAAUCUAUAGGGGAUGUGUCCAGACGACUCGCCCGA 2959_ .H32
TTCG G TA A A ATTGG ATGCTG GGGAGGACGAUGCGGUUCGGUAAAAUUGGAUGCUGCAGACGACUCGCCCGA 2960. .H32
GACCTATCATAATACACCGC GGGAGGACGAUGCGGGACCUAUCAUAAUACACCGCCAGACGACUCGCCCGA 2961. _H32
CTATTGGCGAAGGGTGGTG GGGAGGACGAUGCGGCUAUUGGCGAAGGGUGGUGCAGACGACUCGCCCGA 2962. .H32
CTCTG G ATGTC AGCTG TGTG GGGAGGACGAUGCGGCUCUGGAUGUCAGCUGUGUGCAGACGACUCGCCCGA 2963.. .H32
AGCGCACAACCCTTACGCGT GGGAGGACGAUGCGGAGCGCACAACCCUUACGCGUCAGACGACUCGCCCGA 2964. .H32
TCACTGCAGATAGCCCGCTG GGGAGGACGAUGCGGUCACUGCAGAUAGCCCGCUGCAGACGACUCGCCCGA 2965. .H32
CTATCAG GAAACG ATGTG GC GGGAGGACGAUGCGGCUAUCAGGAAACGAUG UGGCCAGACGACUCGCCCGA 2966. H32
ATTCCTCTCAGCTCACTCCC GGGAGGACGAUGCGGAUUCCUCUCAGCUCACUCCCCAGACGACUCGCCCGA 296? H 32
# in H4
HTS read variable region RNA sequences # in H 3 selection Selected sequenc
TCCATCQTTATG CCGTTCCG GGGAGGACGAUGCGGUCCAUCGUUAUGCCGUUCCGCAGACGACUCGCCCGA 2968_ H32
TTAGGTGTAGGGTGGTACTG GGGAGGACGAUGCGGUUAGGUGUAGGGUGG UACUGCAGACGACUCGCCCGA 2969_ H32
TACCGTCCAATTTCACTGCC GGGAGGACGAUGCGGUACCGUCCAAUU UCACUGCCCAGAC6ACUCGCCCGA 2970„ H32
GAACTGTGAAATGTCGCCTG GGGAGGACGAUGCGGGAACUGUGAAAUGUCGCCUGCAGACGACUCGCCCGA 2971_ H32
TCTGGAATACAACCTGTGCC GGGAGGACGASJGCGGUCUGGAAUACAACCUGUGCCCAGACGACUCGCCCGA 2972_ H32
GCATGGTGTTTGTAGGTGTG GGGAGGACGAUGCGGGCAUGGUGU UUGUAGGLGUGCAGACGACUCGCCCGA 2973_ H32
AATGTGCTCCGCTCCATGGT GGGAGGACGAUGCGGAAUG UGCUCCGCUCCAUGGUCAGACGACUCGCCCGA 2974 _ H32
GTGTCTAAGGGTTGCGTGCC GGGAGGACGAUGCGGGUGUCUAAGGGUUGCGUGCCCAGACGACUCGCCCGA 2975„ H32
CTTG ACGTCTTGTTCG TCCC GGGAGGACGAUGCGGCU UGACG UCUUGUUCGUCCCCAGACGACUCGCCCGA 2976_ H32
TTATTCCG TCCTACGTGCCC GGGAGGACGAUGCGGUUAU UCCGUCCUACGUCCCCCAGACGACUCGCCCGA 2977_ H32
AGCGTAGTTATGGCGTGGGC GGGAGGACGAUGCGGAGCGUAGUUAUGGCGU6GGCCAGACGACUCGCCCGA 2978_ H32
ACCGCTCGTAGATTTGTGCC GGGAGGACGAUGCGGACCGCUCGUAGAUUUGUGCCCAGACGACUCGCCCGA 2979_ H32
TACTATGTGTAGTCCATCTG GGGAGGACGAUGCGGUACUAUGUGUAGUCCAUCUGCAGACGACUCGCCCGA 2980_ H32
TTGATCAGAGGTGGTCTGTA GGGAGGACGAUGCGGUUGAUCAGAGGUGGUCUGUACAGACGACUCGCCCGA 2981_ .H32
TAACTTGTGCGATCGATGTG GGGAGGACGAUGCGGUAACUUGUGCGAUCGAUGUGCAGACGACUCGCCCGA 2982_ H32
TGATTAACTTTAACTGTGTG GGGAGGACGAUGCGGUGAUUAACUUUAACUGUGUGCAGACGACUCGCCC6A 2983_ H32
TCGGCTACGTACAATGTTGC GGGAGGACGAUGCGGUCGGCUACGUACAAUGUUGCCAGACGACUCGCCCGA 2984 , H32
CAGGAACGTCTGTTTGTCCC GGGAGGACGAUGCGGCAGGAACGUCUGUUUGUCCCCAGACGACUCGCCCGA 2985_ H32
TAGAGATAAGAAGTTGCTGT GGGA6GACGAUGCGGUAGAGAUAAGAAGUUGCUGUCAGACGACUC6CCCGA 2986_ H32
TAAGTCATCTATACG CTCCC GGGAGGACGAUGCGGUAACUCAUCUAUACGCUCCCCAGACGACUCGCCCGA 2987„ H32
TCATCCTCCTGTCCTGTGTG GGGAGGACGAUGCGGUCAUCCUCCUGUCCUGUG UGCAGACGACUCGCCCGA 2988_ H32
AACTCGAGTCGCCATTTGTG GGGAGGACGAUGCGGAACUCGAG UCGCCAUUUG UGCAGACGACUCGCCCGA 2989_ H32
TTTGAAGGGTGATGGGGGCG GGGAGGACGAUGCGGUUUGAAGGG UGAUGGGGGCGCAGACGACUCGCCCGA 2990„ H32
ATAGGGAGAAGGGTTAACCG GGGAGGACGAUGCGGAUAGGGAGAAGGGUUAACCGCAGACGACUCGCCCGA 2991_ H32
GTCCTGTCGTCTGTTCGTCAC GGGA6GACGAUGCGGGUCCUGUCGUCUGU UCGUCACCAGACGACUCGCCCGA 2992_ H32
TCTCTGTGGATTTTGCACCC GGGAGGACGAUGCGGUCUCUGUGGAUUUUGCACCCCAGACGACUCGCCCGA 2993_ H32
CATACATGCGTCTCTACCGT GGGAGGACGAUGCGGCAUACAUGCGUCUCUACCG UCAGACGACUCGCCCGA 2994_ H32
ATTGGTGAAGGGATACTG GGGAGGACGAUGCGGAUUGGUGAAGGGAUACUGCAGACGACUCGCCCGA 2995 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTCAAGGGTGATGGGTCCG GGGAGGACGAUGCGGU UCAAGGGUGAUGGG UCCGCAGACGACUCGCCCGA 2996. „H32
CTTAGTGTCTCTGGTATGAC GGGAGGACGAUGCGGCUUAGUGUCUCUGGUAUGACCAGACGACUCGCCCGA 2997. .H32
TGGACCTTAGACAGCCCGTC GGGAGGACGAUGCGGUGGACCUUAGACAGCCCGUCCAGACGACUCGCCCGA 2998. .H32
CCG CACGTCTTTTCGTACCG GGGAGGACGAUGCGGCCGCACGUCUUUUCGUACCGCAGACGACUCGCCCGA 2999. .H32
TTTG AAG G G TG ATTG G CCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUUG6CCCGCAG CGACUCGCCC6A 3000. .H32
CAACGCATCAA6TTGTGCGC GGGAGGACGAUGCGGCAACGCAUCAAGUUG UGCGCCAGACGACUCGCCCGA 3001. _H32
TTTGAAGGGTGGGGGTCCG GGGAGGACGAUGCGGUUUGAAG6GUGGGGGUCC6CAGACGACUCGCCCGA 3002. .H32
AAACTCCTAGTCATGTGCCC GGGAGGACGAUGCGGAAACUCCUAGUCAUG UGCCCCAGACGACUCGCCCGA 3003. H32
TCCTGTCGTCTTTGCGTCC GGGAGGACGAUGCGGUCCUG UCGUCUUUGCGUCCCAGACGACUCGCCCGA 3004. H32 2652 H04
GTCACTTAACACTAATGTAC GGGAGGACGAUGCGGG UGXCUUAACACUAAUGUACCAGACGACUCGCCCGA 3005. H32
AACATTGGTATAAATGTGCC GGGAGGACGAUGC6GAACAUUGGUAUAAAUGU6CCCAGACGACUCGCCCGA 3006. .H32
TCCTTCAAGCCTTCCCCTGGC GGGAGGACGAUGCGGUCCUUCAAGCCUUCCCCUGGCCAGACGACUCGCCCGA 3007. .H32
TTTGAAGGGTGAT6GTCCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUGGUCCCGCAGACGACUCGCCCGA 30Q8. H32
•J\
ACGTGCGCT GACCCACTT6C GGGAGGACGAUGCGGACGUGCGCUGACCCACUUGCCAGACGACUCGCCCGA 3009„ ,H32
A CTTAG T AACT AT AATG CTG GGGAGGACGAUGCGGACUUAGUAACUAUAAUGCUGCAGACGACUCGCCCGA 3010_ H32
TTTGAAGGGTGCTGGTTCCG GGGAGGACGAUGCGGUUUGAAGGGUGCUGGUUCCGCA6ACGACUCGCCCGA 3011. H32 4938 H04
G AATATTTG AC ACCG CG CTG GGGAGGACGAUGCGGGAAUAUUUGACACCGCGCUGCAGACGACUCGCCCGA 3012. .H32
GCCTTTCGGTCTGGCCTCCC GGGAGGACGAUGCGGGCCUUUCGGUCUGGCCUCCCCAGACGACUCGCCCGA 3013. H32
TGAGTCCTGGTCACTGTTGT GGGAGGACGAUGCGGUGAG UCCUGGUCACUGU UGUCAGACGACUCGCCCGA 3014. H32
TACGAGCATGTGACGTTGCC GGGAGGACGAUGCGGUACGAGCAUGUGACG UUGCCCAGACGACUCGCCCGA 3015_ H32
CTGG ATTGCACACTGTACTG GGGAGGACGAUGCGGCUGGAUUGCACACUGUACUGCAGACGACUCGCCCGA 3016_ H32
TTTG A AG G G TTATG G G G CCG GGGAGGACGAUGCGGUUUGAAGGGUUAUGGGGCCGCAGACGACUCGCCCGA 3017. H32 3546 H04
A AGTCTGTGATTGTT6CGT GGGAGGACGAUGCGGAUAG UCUGUGAUUGUUGCGUCAGACGACUCGCCCGA 3018. H32
TACGACATAACCTCTTTGGC GG G AG G ACG A UG CG G UACGACAU AACC UC U U UG G CCAG ACG ACU CG CCCG A 3019. H32
TCGTGTTGGATTCTTCCCCC GGGAGGACGAU6CGGUCGUGUUGGAUUCUUCCCCCCAGACGACUCGCCCGA 3O20„ H32
CGTCAACTTGAACATGTCGC GGGAGGACGAUGCGGCGUCAACUUGAACAUGUCGCCAGACGACUCGCCCGA 3021_ .H32
AACG CCTCCTG TGTCG TG AT GGGAGGACGAUGCGGAACGCCUCCUGUGUCG UGAUCAGACGACUCGCCCGA 3022. .K32
TGACTCGTCTGTTTCGTCTC GGGAGGACGAUGCGGUGACUCGUCUGUUUCGUCJCCAGACGACUCGCCCGA 3023 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ACCGAACCTATCGCTAGCTG GGGAGGACGAUGCGGACCGAACCUAUCGCUAGCUGCAGACGACUCGCCCGA 3024_ H32
TACGTAATG AAATACTG CCC GGGAGGACGAUGCGGUACGUAAUGAAAUACUGCCCCAGACGACUCGCCCGA 3025, ,H32
TTG ATTCGAGGTGACAG CCC GGGAGGACGAUGCGGU UGAUUCGAGGUGACAGCCCCAGACGACUCGCCCGA 3026.. _H32
ACAGCTTATGACACCCTCCC GGGAGGACGAUGCGGACAGCUUAUGACACCCUCCCCAGACGACUCGCCCGA 3027_ .H32
CTCAACGGACCATTGGCCAG GGGAGGACGAUGCGGCUCAACGGACCAUUGGCCAGCAGACGACUCGCCCGA 3028. „H32
ATCGACACGAGTTGTTCGTG GGGAGGACGAUGCGGAUCGACACGAGUUGUUCGUGCAGACGACUCGCCCGA 3029. ,H32
AAC6ATC AG CTACGTTG CGC GGGAGGACGAUGCGGAACGAUCAGCUACGUUGCGCCAGACGACUCGCCCGA 3030_ H32
CG1TTCCTCTGGTTAGTCCC GGGAGGACGAUGCGGCG UUUCCUCUGGUUAG UCCCCA6AC6ACUCGCCCGA 3031_ _H32
TTGGGTGTGGGGAGGTACCG GG6AGGACGAUGCGGU UGGGUGUGGGGAGGUACCGCAGACGACUCGCCCGA 3032_ _H32
GCCTTTCGTTCTGTCCTCTC GGGAGGACGAUGCGGGCCUUUCGUUCUGUCCUCUCCAGACGACUCGCCCGA 3033_ _H32
TACAACGCGAAACCTCTGTG GGGAGGACGAUGCGGUACAACGCGAAACCUCUGUGCAGACGACUCGCCCGA 3034_ .H32
CTTGAATGGTGATGGGTCCG GGGAGGACGAUGCGGCU UGAAUGG UGAUGGGUCCGCAGACGACUCGCCCGA 3035_ .H32
AG AGTGTATCTG CTAJTATG GGGAGGACGAUGCGGAGAGUGUAUCUGCUAU UAUGCAGACGACUCGCCCGA 3036_ _H32
AGGACGACTGCGATATGCTG GGGAGGACGAUGCGGAGGACGACUGCGAUAUGCUGCAGACGACUCGCCCGA 3037_ _H32
CTGGATGGCGCAAGCATGTG GGGAGGAC6AUGCGGCU6GAUGGCGCAAGCAUG UGCAGACGACUCGCCCGA 3038_ _H32
TCGGAAAGCTGCTTAGCCGC GGGAGGACGAUGCGGUCGGAAAGCUGCUUAGCCGCCAGACGACUCGCCCGA 3039_ _H32
GACCCATCCA'CTAACCTGTG GGGAGGACGAUGCGGGACCCAUCCACUAACCUGJGCAGACGACUCGCCCGA 3040.. ,H32
CCTTTGTTTCTGG GTATGCC GGGAGGACGAUGCGGCCUUUGU UUCUGGGUAUGCCCAGACGACUCGCCCGA 3041. ,H32
TAAGTAGTTTGCCAACTTGG GGGAGGACGAUGCGGUAAG UAG UU UGCCAACUUGGCAGACGACUCGCCCGA 3042_ _H32
CG AGTGTTCTGATGTCCCCC GGGAGGACGAUGCGGCGAGUGUUCUGAUGUCCCCCCAGACGACUCGCCCGA 3043_ _H32
TGTTG A AGG GTGATG GGTCC GGGAGGACGAUGCGGUGUUGAAGGGUGAUGGGUCCUCAGACGACUCGCCCGA 3044_ „H32 4708 H04
TTACCTCCGTTTTGTGGCCC GGGAGGACGAUGCGGU UACCUCCGUUUUG UGGCCCCAGACGACUCGCCCGA 3045_ _H32
CGGTGGATGTTGTCTGTGTG GGGAGGACGAUGCGGCGGUGGAUGUUGUCUGUG UGCAGACGACUCGCCCGA 3046. _H32
GG CCC ATTAG TCAGTCTGTG GGGAGGACGAUGCGGGGCCCAUUAGUCAGUCUGUGCAGACGACUCGCCCGA 3047.. .H32
CGTTTCCTCTGGTTCGTTTC GGGAGGACGAUGCGGCGUU UCCUCUGGUUCGU UUCCAGACGACUCGCCCGA 3048, .H32
TTTGGGGTTiTCCGGTGCCC CGGAGGACGAUGCGGUUUGGGGUUUUCCGG UGCCCCAGACGACUCGCCCGA 3049_ „H32
GCG AATACA GCTCTTGGTG C GGGAGGACGAUGCGGGCGAAUACAGCUCUUGGUGCCAGACGACUCGCCCGA 3050. .H32
TGG TCGTGTGTACGTCTG GC GGGAGGACGAUGCGGUGGUCGUGUGUACGUCUGGCCAGACGACUCGCCCGA 3051 H32
in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ACGTCCTTTCTAT6G CTGTG GGGAGGACGAUGCGGACGUCCUUUCUAUGGCUGUGCAGAC6ACUCGCCCGA 3052_ _H32
ACGTG GTG TAG G G G G G AGTC GGGAGGACGAUGCGGACGUGGUGUAGGGGGGAG UCCAGACGACUCGCCCGA 3053. .H32
CATG CAATG AGTG G CACCTG GGGAGGACGAUGCGGCAUGCAAUGAGUGGCACCUGCAGACG ACUCGCCCGA 3054. .H32
AG CTG GTA AACTG TCG CCG GGG AGGACGAUGC6GAGCUGGUAAACUGUCGCCGCAGACGACUCGCCCGA 3055. ..H32
GTGTCGT ACTGG CGTTG GTG GGGAGGACGAUGCGGG UGUCG UACUGGCG U UGG UGCAGACG ACUCGCCCGA 3056_ .H32
TTTGACGTGTGATGGGTCCG GGGAGGACGAUGCGGUUUGACGUGUGAUGGGUCCGCAGACG ACUCGCCCGA 3057. _H32
CAAAGTATAGACTGTGGATG GGGAGGACGAUGCGGCAAAGUAUAGACUGUGGAUGCAGACGACUCGCCCGA 3058. .H32
TTTGAGGGGTGATGGGTCGG GGGAGGACGAUGCGGUUUGAGGGGUGAUGGGUCGGCAGACGACUCGCCCGA 3059. .H32
TGGGTTCTATCGACCGTGTG GGGAGGACGAUGCGGUGGG UUCUAUCGACCGUGUGCAGACGACUCGCCC6A 3060. _H32
TTTCGTGTCCACTCACCCCA GGGA6GACGAUGCGGUUUCGUGUCCACUCACCCCACAGACGACUCGCCCGA 3061. H32
TAGTAAACCTCTCGTGTAGC GGGAGGACGAUGCGGUAGUAAACCUCUCGUGUAGCCAGACG ACUCGCCCGA 3062. _H32
TTGGGTGGGGGATGGGACCG GGGAGGAC6AUGCGGU UGGGUGGGGGAUGGGACCGCAGACG ACUCGCCCGA 3063. _H32
AACCGCACAGTGACCAGCCC GGGAGGACGAUGCGGAACCGCACAGUGACCAGCCCCAGACGACUCGCCCGA 3064. _H32
'Jl
ACTCAAAGTCTCCCG TTCTC GGGAGGACGAUGCGGACUCAAAGUCUCCCGUUCUCCAGACGACUCGCCCGA 3065. _H32
GAGAACAATGGTGTGTTTGC GGGAGGACGAUGCGGGA6AACAAUGGUGUGUUUGCCAGACGACUCGCCCGA 3066. .H32
TTTAGGGTTAATG CGGG GTC GGGAGGACGAUGCGGUUUAGGGUUAAUGCGGGG UCCAGACGACUCGCCCGA 3067.. .H32
TCATGGCAGCACCCGAGCTG GGGAGGACGAUGCGGUCAUGGCAGCACCCGAGCUGCAGACGACUCGCCCGA 3068. _H32
TTTGAAGGGTGTTGGGTCAG GGGAGGACGAUGCGGU UUGAAGGGUGUUGGGUCAGCAGACGACUCGCCCGA 3069. H32
TTGGGTGTAGAGAGGTACCG GGGAGGACGAUGCGGU UGGGUGUAGAGAGGUACCGCAGACG ACUCGCCCGA 3070. .H32
GTTCTCCGeCCGATTGTGGC GG6AGGACGAUGCGGGUUCUCCGGCCGAUUGUGGCCAGACGACUCGCCCGA 3071. ,H32
CCTGGTAACCG AT A ATG C CC GGGAGGACGAUGCGGCCUGGUAACCGAUAAUGCCCCAGACGACUCGCCCGA 3072. H32
AAACGAGCGACCGTCACCTA GGGAGGACGAUGCGGAAACGAGCGACCGUCACCUACAGACGACUCGCCCGA 3073. „H32
TAAGTCTTACCAATCGTGTG GGGAGGACGAUGCGGUAAGUCUUACCAAUCGUGUGCAGACGACUCGCCCGA 3074. .H32
CTAA G AACCTAACGTCCATG GGGAGGACGAUGCGGCUAAGAACCUAAC6UCCAUGCAGACGACUCGCCCGA 3075. _H32
CATACCTCQTCCAGACTGTG GGGAGGACGAUGCGGCAUACCUCGUCCA6ACUGUGCAGACGACUCGCCCGA 3076. _H32
ACCACACACTACGCGAGCTG GGGAGGACGAUGCGGACCACACACUACGCGAGCUGCAGACGACUCGCCC.6A 3077. _H32
TACTGCGCTGTACTCGTCCC GGGAGGACGAUGCGGUACUGCGCUGUACUCGUCCCCAGACGACUCGCCCGA 3078, .H32
CACAGGAGAAGGGAGGTTGC GGGAGGACGAUGCGGCACAGGAGAAGGGAG6UUGCCAGACG ACUCGCCCGA 3079 H32
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
AGCCGATCTTGCCGTTTGCC GGGAGGACGAUGCGGAGCCGAUCUUGCCGUUU6CCCAGACGACUCGCCCGA 3080. _H32
CATTGACTTCCGTTGCCCCC GGGAGGACGAUGCGGCAUUGACUUCCGUUGCCCCCCAGACGACUCGCCCGA 3081_ _H32
ACG GGTACTTGGTCTGTGTG GGGAGGACGAUGCGGACGGG UACUUGGUCUG UGUGCAGACGACUCGCCCGA 3082, _H32
TAACTGTCGACAACCCCGTC GGGAGGACGAUGCGGUAACUGUCGACAACCCCGUCCAGACGACUCGCCCGA 3083_ .H32
TTGGAAGGAGGATGGGTCCG GGGAGGACGAUGCGGUUGGAAGGAGGAUGGGUCCGCAGACGACUCGCCCGA 3084_ .H32
TTCGAAGGAT-GATGGGTCCG GGGAGGACGAUGCGGUUCGAAGGAUGAUGGGUCCGCAGACGACUCGCCCGA 3085. .H32
TATTAATGCTAAAGTGTCCC GGGAGGACGAUGCGGUAUUAAUGCUAAAG UGUCCCCAGACGACUCGCCCGA 3086. _H32
6TG GGTGTAGGGTGTTACCG GGGAGGACGAUGCGGG UGGGUGUAGGGUGUUACCGCAGACGACUCGCCCGA 3087. _H32
TGAAAAACACATTGTTGCAT GGGAGGACGAUGCGGUGAAAAACACAUUGU UGCAUCAGACGACUCGCCCGA 3088. .H32
TTGGTTGTAGGGGGGTACCG GGGAGGACGAUGCGGU UGGUUGUAGGGGGG UACCGCAGACGACUCGCCCGA 3089. .H32
ACGCCGCTATTCACTCCC GGGAGGACGAUGCGGACGCCGCUAUUCACUCCCCAGACGACUCGCCCGA 3090. .H32
AAACAGGCAAAAAGCTTGTA GGGAGGACGAUGCGGAAACAGGCAAAAAGCUUGUACAGACGACUCGCCCGA 30 1_ _H32
TCATGTGAGAACGATCCCCC GGGAGGACGAUGCGGUCAUG UGAGAACGAUCCCCCCAGACGACUCGCCCGA 3092. _H32
•J\
CGACCTCTGAGTTG CGTG AC GGGAGGACGAUGCGGCGACCUCUGAGUUGCGUGACCAGACGACUCGCCCGA 3093_ H32
TCTTTTCTCCCTGCCCTCTC GGG'AGGACGAUGCGGUCUUUUCUCCCUGCCCUCUCCAGACGACUCGCCCGA 3094. „H32
ACCTTCG CAG CTGG TTTCGC GGGAGGACGAUGCGGACCUUCGCAGCUGGUUUCGCCAGACGACUCGCCCGA 30S5. .H32
GTATACAGAAATTTGCCCGT GGGAGGACGAUGCGGGUAUACAGAAAUUUGCCCG UCAGACGACUCGCCCGA 3096_ .M32
TCCGCCGAGTTTTCCCCCTA GGGAGGACGAUGCGG UCCGCCGAG UUUUCCCCCUACAGACGACUCGCCCGA 3097. _H32
CGTAAAAAGTCGGTGGTCGC GGGAGGACGAUGCGGCGUAAAAAGUCGGUGGUCGCCAGACGACUCGCCCGA 3098. mi
TTCGTAGGGTGATGGGTCCG GGGAGGACGAUGCGGUUCGUAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 3D99_ _H32
CCGTATCTTCTCTGTCCCCC GGGAGGACGAUGCGGCCGUAUCUUCUCUGUCCCCCCAGACGACUCGCCCGA 3100. H32
AATAC A GTCTCTTTTCG CCG GGGAGGACGAUGCGGAAUACAGUCUCUUUUCGCCGCAGACGACUGGCCCGA 3101. H32
CAATCATCGCATGTCTGGGC GGGAGGACGAUGCGGCAAUCAUCGCAUGUCU6GGCCAGACGACUC6CCCGA 3102. H32
TCCCCTAATTTCTGTCGCCG GGGAGGACGAUGC6GUCCCCUAAUUUCUGUCGCCGCAGACGACUCGCCCGA 3103. H32
CCTGGAGAGCATCGTTGGTG GGGAGGACGAUGCGGCCUGGAGAGCAUCGUUG UGCAGACGACUCGCCCGA 3104. H32
TACCAATTAGTTATGACCGC GGGAGGAC6AUGCGGUACCAAUUAGUUAUGACCGCCAGACGACUCGCCCGA 3105. H32
ATCTACACTGAGGTGGTGTA GGGAGGACGAUGCGGAUCUACACUGAGGUGGUGUACAGACGACUCGCCCGA 3106. .H32
GGGAGGACGAUGCGGUUGCGGUGUAGGGUGG UACCGCAGACGACUCGCCCGA 3107 H32
n in
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TCGTTGGGTCGGTGCTGCAC GGGAGGACGAUGC6GUCGUUGGGUCGGUGCUGCACCAGACGACUCGCCCGA 3108. .H32
ATTGCTCTTCTCTGCCGCAC GGGAGGACGAUGCGGAUUGCUCUUCUCUGCCGCACCAGACGACUCGCCCGA 3109. .H32
TGACCTGTGTCATG CCCCAC GGGAGGACGAUGCGGUGACCUGUGUCAUGCCCCACCAGACGACUCGCCCGA 3110. .H32
TTGGGTGTAGTGAGGTACCG GGGAGGACGAUGCGGUUGGGUGUAGUGAGGUACCGCAGACGACUCGCCCGA 3111. .H32
TAGGTAACAAATTACACCTG GGGAGGACGAUGCGGUAGGUAACAAAUUACACCUGCAGACGACUCGCCCGA 3112. .H32
TTTG AAGT6TG ATGG GGCCG GGGAGGACGAUGCGGUUUGAAGUGUGAUGGGGCCGCAGACGACUCGCCCGA 3113. „H32
TTTGGTGGAGGATGGGACCG GGGAGGACGAUGCGGUUUGGUGGAGGAUGGGACCGCAGACGACUCGCCCGA 3114. _H32
ATTC ACG ATCTGTCG CTG CC GGGAGGACGAUGCGGAUUCACGAUCUGUCGCUGCCCAGACGACUCGCCCGA 3115. .H32
GTCCTAGTTCTGTCCCTCAC GGGAGGACGAUGCGGGUCCUAGUUCUGUCCCUCACCAGACGACUCGCCCGA 3116. .H32
CAAGG GCG AAACCCTATCTG GGGAGGACGAUGCGGCAAGGGCGAAACCCUAUCUGCAGACGACUCGCCCGA 3117. _H32
TTTCTATG GATGATTCCGTG GGGAGGACGAUGCGGUUUCUAUGGAUGAUUCCGUGCAGACGACUCGCCCGA 3118. .H32
TTTGCAGGGTGATGGCTCCG G GGAGG ACG AU G CG G ϋ UU G CAG G GU G AU GG C UCCGCAG ACG AC UC G CCCG A 3119. _H32
TCGCTTGCACAATTTTTGGC GGGAGGACGAUGCGGUCGCUUGCACAAUU U'JUGGCCAGACGACUCGCCCGA 3120. _H32
ο CCACTGTCACTCCCTGTCCG GGGAGGACGAUGCGGCCACUGUCACUCCCUG UCCGCAGACGACUCGCCCGA 3121. .H32
AAAAATCTCCTTCACCGGTG GGGAGGACGAUGCG6AAAAAUCUCCUUCACCGGUGCAGACGACUCGCCCGA 3122. _H32
ACCAGTAACCGTACGCGCTG GGGAGGACGAUGCGGACCAG UAACCGUACGCGCUGCAGACGACUCGCCCG 3123. .H32
CCTACG ACCAGTACGTG CTG GGGAGGACGAUGCGGCCUACGACCAGUACGUGCUGCAGACGACUCGCCCGA 3124. _H32
ATTGTCTATGTTTAC ATG G C GGGAGGACGAUGCGGAUUGUCUAUGUUUACAUGGCCAGACGACUCGCCCGA 3125. ,H32
TGCGATACGACATTTCTCCC GGGAGGACGAUGCGGUGCGAUACGACAUUUCUCCCCAGACGACUCGCCCGA 3126. ,H32
TTGG GTGTAG AG CG GTAC CG GGGAGGACGAUGCG6UUGGGUGUAGAGCGG UACCGCAGACGACUCGCCCGA 3127. .H32
TTTTTG A AGG GIG ATG G GTC CT GGGAGGACGAUGCGGU UUUUGAAGGGUGAUGGGUCCUCAGACGACUCGCCCGA 3128. .H32
CTATG ACG ATCATCCCTG CC GGGAGGACGAUGCGGCUAUGACGAUCAUCCCUGCCCAGACGACUCGCCCGA 3129. .H32
CCG CACTTATACCACCGCTG GGGAGGACGAUGCG6CC6CACUUAUACCACCGCUGCAGACGACUCGCCCGA 3130. ,H32
CGTTAAAGTAATGTGGGCGC GGGAGGACGAUGCGGCG UUA.AAGUAA.UGUGGGCGCCAGACGACUCGCCCGA 3131. H32
GAATTGCCTTCCTGTAGCCC GGGAGGACGAUGCGGGAAU UGCCUUCCU6UAGCCCCAGACGACUCGCCCGA 3132. .H32
TTTGATGGAGGGTSGGACCG GGGAGGACGAUGCGGUUUGAUGGAGG6UGGGACCGCAGACGACUCGCCCGA 3133. H32
GTCTTCAGGCTTATCGTCCC GGGAGGACGAUGCGGGUCU UCAGGCU UAUCGUCCCCAGACGACUCGCCCGA 3134. .H32
ACGAGGTCCAATGGTTTGCC GGGAGGACGAUGCGGACGAGGUCCAAUGGUUUGCCCAGACGACUCGCCCGA 3135 H32
# irs H4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc CTATTGGTGAAGGGCGGTG GGGAGGACGAUGCGGCUAUUGGUGAAGGGCGGUGCAGACGACUCGCCCGA 3136_H32
C AT.CTTC CTG CTCCCTG G C GGGAGGACGAUGCGGCAUCUUCCUGCUCCCUGGCCAGACGACUCGCCCGA 3137_H32
TTTCGTT A ATTG TCGTTGC A GGGAGGACGAUGCGGUUUCGU UAAUUGUCGUUGCACAGACGACUCGCCCGA 3138_H32
GTAT AG AC AT AG G CTCTGTG GGGAG6ACGAUGCGGGUAUAGACAUAGGCUCUGUGCAGACGACUCGCCCGA 3i39_.H32
AAC G GTG AT6GTCCAG TGTC GGGAGGACGAUGCGGAACGGUGAUGGUCCAGUGUCCAGACGACUCGCCCGA 3140_H32
TTTGAAGGGTGATGGGCCC GGGAGGACGAUGCGGUUUGAAGGGUGAUGGGCCCCAGACGACUCGCCCGA 3141..H32
ACC G TTAAG CTCCC C G TTG C GGGAGGACGAUGCGGACCGU UAAGCUCCCCG UUGCCAGACGACUCGCCCGA 3142_ H32
TAAACATCTG CATCACCCCC GGGAGGACGAUGCGGUAAACAUCUGCAUCACCCCCCAGACGACUCGCCCGA 3143_H32
TTTGG6AGTAGGGTGTTCCG GGGAGGACGAUGCGGU UU6GGAGUAGGGUG UUCCGCAGACGACUCGCCCGA 0005. H04
GTTGGTGTAGGGGGGTTCCG GGGAGGACGAUGCGGGUUGGUGUAGGGGGGUUCCGCAGACGACUCGCCC'GA 0020. H04
ATGCTTGGAGTAGGGAGGTG GGGAGGACGAUGCGGAUGCU UGGAGUAGGGAGGUGCAGACGACUCGCCCGA 0022. H04
CATAGGAGTAGGGTGGTAGC GGGAGGACGAU6CGGCAUAGGAGUAGGGUGG UAGCCAGACGACUCGCCCGA 0034_ H04
AAAGG AG G AG TTG G G ATCT6 GGGAGGACGAUGCGGAAAGGAGGAG UUGGGAUCUGCAGACGACUCGCCCGA 00?»9_ H04
CTTATG 6 A G TAG G GTG GTG C GGGA6GACGAUGCGGCUUAUGGAGUAGGGUGGUGCCAGACGACUCGCCCGA 0048. H04
ATTCACTTCTTTTCTTG CCG GGGAGGACGAUGCGGAUUCACUUCUUUUCUUGCCGCAGACGACUCGCCCGA 0049. H04
ATTTG G AGG AG G AG G TTCCG GGGAGGACGAUGCGGAU UUGGAGGAGGAGGU UCCGCAGACGACUCGCCCGA 0057_ H04
TrrGTTTTTCTCTTTTTC C G GGGAGGACGAUGCGGUUUG UU UUUCUCUUU UUCCGCAGACGACUCGCCCGA 0060, H04
TTTATTTCTTTCCAC G TTCCG GGGAGGAC6AUGCGGUUUAUUUCUUUCCACGU UCCGCAGACGACUCGCCC6A 0065, H04 U5
TTCTTTA'rnTTTCCCTCCG GGGAGGACGAUGCGGUUCUUUAUUUUUUCCCUCCGCAGACGACUCGCCCGA 0073.. H04
CCTTTTACTTTTTTTCTCCG GG GAG GACG A UG CG G CC U U U UAC UU U UU U UC UCCG CAG ACGACUCG CCCG A 0076. H04
TTTTATTCTTTTCCGCTCCG GGGAGGACGAUGCGGUUUUAUUCUUU UCCGCUCCGCAGACGACUCGCCCGA 0077_ H04
AATTAT6TGTTTTTTGTGACCG GGGAG6ACGAUGCGGAAUUAUG UGUUUUUUGUGACCGCAGACGACUCGCCCGA 0078. H04
ATCTTTTCTTTCTTCTGCCG GGGA6GACGAUGCGGAUCUU UUCUUUCUUCUGCCGCAGACGACUCGCCCGA 0081. H04 KU4
CTTATGGAGTAGGGAGGTGC GGGAGGACGAUGCGGCUUAUGGAGUAGGGAGGUGCCAGACGACUCGCCCGA 0089. H04
TCTTTTCATG TTTCTCTC CG GGGAGGACGAUGCGGUCUUUUCAUG UUUCUCUCCGCAGACGACUCGCCCGA 0094. H04
TTTTTCTTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUUUCU UUUCCUUUGUCC6CAGAC6ACUCGCCCGA 0098. H04
ATTACTC'ITCTCTTTTGCCG GGGAGGACGAUGCGGAUUACUCUUCUCUUUUGCCGCAGACGACUCGCCCGA 0100. H04
TTTGTTTTA i 1 1 i 1 i GTCCC GGGAGGACGAUGCGGUUUG UUUUAUUUUUUGUCCCCAGACGACUCGCCCGA 0101 H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
AAGGGTGTAGGGTGTTCCCG GGGAGGACGAUGCGGAAGGGUGUAGGGUG UUCCCGCAGACGACUCGCCCGA 0107_H04
ATTTCTTTTCTCTGTTGCCG GGGAGGACGAUGCGGAUUUCU UUUCUCUGU UGCCGCAGACGACUCGCCCGA 0112_H04
ATTCCTTTTCTTTTTCTCCG GGGAGGACGAUGCGGAUUCCUUUUCUUUUUCUCCGCAGACGACUCGCCCGA 0116_H04
TTATTTTCCTTTTTCTGCCG GGGAGGACGAUGCGG UUAUUUUCCUUU UUCUGCCGCAGACGACUCGCCCGA Q11S_HQ4
ATTCTTTTCTCTTTTCACCG GGGAGGACGAUGCGGAUUCUU UUCUCUU UUCACCGCAGACGACUCGCCCGA 0113_H04
TTTGTTAATTCTTGTTTCCG GGGAGGACGAUGCGGUUUGUUAAUUCUUG UUUCCGCAGACGACUCGCCCGA 0120_H04 KU6
ATTTCTTTTCCTTTTTACCG GGGAGGACGAUGCGGAUU UCUUUUCCUUUUUACCGCAGACGACUCGCCCGA 0124_H04
ATTCTC1TTCTTTTTCACCG GGGAGGACGAUGCGGAUUCUCUUUCUUU UUCACCGCAGACGACUCGCCCGA 0128_H04
ATTTCTCTTCTTCTTTGCCG GGGAGGACGAUGCGGAUU UCUCUUCUUCUUUGCCGCAGACGACUCGCCCGA O13OJH04
TTTGTTTATTCn I I I I' CCG GGGAGGACGAUGCGGU UUGUUUAUUCUUUUUUCCGCAGACGACUCGCCCGA 0131_H04
ATC7TTCTTATTTTCTGGCG GGGAGGACGAUGCGGAUCU UUCUUAUUUUCUGCCGCAGACGACUCGCCCGA 0132_HG4
TCTTTTTAACTTTTTCTCCG GGGAGGACGAUGCG6UCUUUUUAACUUUUUCUCCGCAGACGACUCGCCCGA Q134_H04
ACTTTCTTCTCTTCTTGCCG GGGAGGACGAUGCGGACIJUUCUUCUCUUCUUGCCGCAGACGACUCGCCCGA 0135_H04
CTTTCAC i I ! i I I rCTCTCCG GGGAGGACGAUGCGGCUUUCACUUUUUUUCUCUCCGCAGACGACUCGCCCGA 0137_H04
ATTTTCTCTTTTG CTTG CCG GGGAGGACGAUGCGGAUUU UCUCUU UUGCUUGCCGCAGACGACUCGCCCGA 0139_H04
TAAGGAGGAGTTGGGAACCG 66GAGGACGAUGCGGUAAGGAGGAGUUGGGAACCGCAGACGACUCGCCCGA 0140_H04
ATTCTTGCTTTTTTTGCCG GGGAGGACGAUGCGGAUUCUUGCUUUUUUUGCCGCAGACGACUCGCCCGA Q141_H04
TTTTTCATTCCCTTTTTCCG GGGAGGACGAUGCGGUUUUUCAUUCCCUUUUUCCGCAGACGACUCGCCCGA 0145_H04
TTTTCACTCTCTTCTCTCCG GGGAGGACGAUGCGGUUUUCACUCUCUUCUCUCCGCAGACGACUCGCCCGA 0146_H04
ATTTTCTTCTTCTTTTACCG GGGAGGACGAUGCGGAUUUUCUUCUUCUUUUACCGCAGACGACUCGCCCGA G148_ H04
TCTTTACTTTCTCTTTGCCG GGGAGGACGAUGCGGUCUUUACUUUCUCUUUGCCGCAGACGACUCGCCCGA 0149_H04
TTTCTTGTCTATTTTTTCCG GGGAGGACGAUGCGGUUUCUUGUCUAUUUULfUCCGCAGACGACUCGCCCGA Q151_H04
ATTTTCTCTTTTTTCTG CCG GGGAGGACGAUGCGGAUU UUCUCUUUUUUCUGCCGCAGACOACUCGCCCGA 0155,_H04
ACTTCTCTTGTCTTCTTCCG GGGAGGACGAUGCGGACUUCUCUIJGUCU UCUUCCGCAGACGACUCGCCCGA 0156_H04
TCTTTTGTTATTTTCATCCG GGGAGGACGAUGCGGUCUU UUGUUAtJUUUCAUCCGCAGACGACUCGCCCGA 0157_HQ4
CTTTTCTTCGCTTTTTACCG GGGAGGACGAUGCGGCUUU UCULSCGCUUUUUACCGCAGACGACUCGCCCGA 0158„H04
TTTCTCTTTTTCTCTTTCCG GGGAGGACGAUGCGGUUUCUCUUUUUCUCUUUCCGCAGACGACUCGCCCGA 0159_K04
CTTTATTTTTG t i I 1 1 I GCCC GGGAGGACGAUGCGGCUUUAUUUUUGUUU UUUGCCCCAGACGACUCGCCCGA 0160 H04
t in H4
HTS read variable region RNA sequences ff in H3 selection selection Selected sequenc
CTTCTTCACTTTTCCCTCC6 GGGAGCACGAUGCCGCUUCU UCACUUUUCCCUCCGCAGACGACUCGCCCGA 0163. .H04-
CTTTTTCTTCTCTTGTTCCG GGGAGGACGAUGCGGCUUUUUCUUCUCUUGU UCCGCAGACGACUCGCCCGA 0165. .H04
ATTCTTCTATTTTCTCACCG GGGA6GACGAUGCGGAUUCUUCUAUUUUCUCACCGCAGACGACUCGCCCGA 0172. .H04
TCTTATTTTTCTCTTCTCCG GGGAGGACGAUGCGGUCUUAUUUUUCUCU UCUCCGCAGACGACUCGCCCGA 0173_ _H04
TTTTGTCATTTCTTGTTCC6 GGGAGGACGAUGCGGUUUUGUCAUU UCUUGUUCCGCAGACGACUCG CCGA 0175. ,H04
TTTATTTTCTCGTTGTTGTG GGGAGGACGAUGCGGUUUAUUUUCUCGUUGUUGUGCAGACGACUCGCCCGA 0178. „H04
ATTTTTCTCTACTTTCACCG GGGAGGACGAUGCGGAUUUUUCUGUACUUUCACCGCAGACGACUCGCCCGA 0179. ^H04
TTCATTTTCTTTTCTCTCCG GGGAGGACGAUGCGGUUCAUUUUCUUUUCUCUCCGCAGACGACUCGCCCGA 0180. .H04
AGAGGTGTAGGGAGGTTCCG GGGAGGACGAUGCGGAGAGGUGUAGGGAGGUUCCGCAGACGACUCGCCC6A 0182. _H04
TTTTTTATG GTTTG TTTCCG GGGAGGACGAUGCGGUUU UUUAUGGUU UGUU UCCGCAGACGACUCGCCCGA 0183. H04
ACTTTTTTTG TCTTCTG CCG GGGAGGACGAUGCGGACUU UUUUUG UCUUCUGCCGCAGACGACUCGCCCGA 0189. _H04
GTTTTCTTGTTTCTTGTTCCG GGGAGGACGAUGCGGGUUUUCUUGUUUCUUGUUCCGCAGACGACUCGCGCGA 0191. _H04
CTTTTTGCTCTTTTTCTCCG GGGAGGACGAUGCGGCUUU UUGCUCUUU UUCUCCGOXGACGACUCGCCCGA 0193. _H04
CTTTTCTTTCTC TCCG GGGAGGACGAUGCGGCUUUUCUUUCUCUUUUUCCGCAGACGACUCGCCCGA 0195. . H04
ATCTTTAT JTCTCTCCG GGGAGGACGAUGCGGAUCU UUAUU UUUUCUCUCCGCAGACGACUCGCCCGA 0196. _H04
TTCACTTTTTCCTCATTCCG GGGAGGACGAUGCGGUUCACUUU.UUCCUCAUUCCGCAGACGACUCGCCCGA 0198,. H04
ACTCTTTCTTCTCTTTGCCG GGGAGGACGAUGCGGACUCUUUCUUCUCUUUGCCGCAGACGACUCGCCCGA 0199. „H04
ATTCCTTTTCTCCTTTGCCG GGGAGGACGAUGCGGAU UCCUUU UCUCCUUUGCCGCAGACGACUCGCCCGA 0200. H04
TCCTGTCGTCTTTCGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUUUCGUCCCCAGACGACUCGCCCGA 0201. H04
ACTTTACTC7TCTTTTACCG GGGAGGACGAUGCGGACUU UACUCUUCUUUUACCGCAGACGACUCGCCCGA 0205. .H04
CTTCTTTAAATTTCCCTCCG GGGAGGACGAUGCGGCUUCUUUAAAUU UCCCUCCGCAGACGACUCGCCCGA 0211. _H04
TATTTTCCTTGTTTTCTCCG GGGAGGACGAU-GCGGUAUUUUCCUUGUUUUCUCCGCAGACGACUCGCCCGA 0214. _H04
TTTTCmCTATTCTTTCCG GGGAGGACGAUGCGGU UU UCUUUCUAUUCUUUCCGCAGACGACLiCGCCCGA 0216. _H04
TTTTTGTTCCCTTTTG CCCG G6GAGGACGAUGCGGU UU UUG UUCCCUU UU6CCCGCAGACGACUCGCCCGA 0217. .ΗΌ4
TGGGTGTAGGGAGGTAACCG GGGAGGACGAUGCGGUGGGUGUAGGGAGGUAACCGCAGACGACUCGCCCGA 0219. _H04
ACTTTTTTCTCTCCTTTCCG GGGAGGACGAUGCGGACUU UUUUCUCUCCUUUC GCAGACGACUCGCCCGA 0222. .H04
mCTTTCCATTTCTTG CCG GGGAGGACGAUGCGGUUUCUUUCCAUUUCUUGCCGCAGACGACUCGCCCGA 0227. _H04
ATTATTT GGGAGGACGAUGCGGAU UAUUUACUUUUUGCCCCGCAGACGACUCGCCCGA 0228 H04
# ίη Η4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc TTTCTTTCGTTTTCTCTCCG GGGAGGACGAUGCGGUUUCUUUCGUUUUCUCUCCGCAGACGACUCGCCCGA 0233_ H04
TCTTTTCTTCTCTTCTACCG GGGAGGACGAUGCGGUCUUUUCU UCUCUUCUACCGCAGACGACUCGCCCGA 0234. _H04
TTTCTTTTACTTTCTTTCCG GGGAGGACGAUGCGGUUUCUUUUACUUUCUUUCCGCAGACGACUCGCCCGA G235„ .H04
ACCTTTTCTTTCCTATTCCG GGGAGGACGAUGCGGACCUUUUCUUUCCUAUUCCGCAGACGACUCGCCCGA 0236. R04
TTTTATTTTCTTTTCCCCCG GGGAGGACGAUGCGGU UUAUUUUCUU UUCCCCCGCAGACGACUCGCCCGA 0237. H04
TTTTTTG ATTCTTGTTTGCG GGGAGGACGAUGCGGUUUUUUGAUUCUUGUUUCCGCAGACGACUCGCCCGA 0238. .H04
TCCTGTCGGCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGGCUGUUCGUCCCCAGACGACUCGCCCGA 0239. H04
TTCATTTGTTCTCTTCTCCG GGGAGGACGAUGCGGUUCAUU UCU UCUCUUCUCCGCAGACGACUCGCCCGA 0241 . .H04
TTTGGGAGTAGGGTGTTCCC GGGAGGACGAUGCGGUUUGGGAGUAGGGUGUUCCCCAGACGACUCGCCCGA 0242. R04
TTTACTTTCCTCTTTTG CCG GGGAGGACGAUGCGGUUUACUUUCCUCUUUUGCCGCAGACGACUCGCCCGA 0243_ .H04
ATCTTTCTTTACTCTCACCG GGGAGGACGAUGCGGAUCU UUCUUUACUCUCACCGCAGACGACUCGCCCGA 0245_ _RQ4
TTTTTCTACTTTCTT TCCG GGGAGGACGAUGCGGUUUUUCUACUUUCUU UUCCGCAGACGACUCGCCCGA 024?_ _H04
TTTCTTTTG CTTTCTCTCCG GGGAGGACGAUGCGGUUUCUUUUGCU UUCUCUCCGCAGACGACUCGCCCGA 025i_ H04
4- TCTTTTTCTACCTTTTTCCG GGGAGGACGAUGCGGUCUU UUUCUACCUUUU UCCGCAGACGACUCGCCCGA 0252_ _H04
TTGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUGGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 0253. .H04
TTTTACTATTCTCCTTTCCG GGGAGGACGAUGCGGUUU UACUAUUCUCCUU UCCGCAGACGACUCGCCCGA 0254_ _H04
ATTTTTTCTTTCCTTTG CCG GGGAGGACGAUGCGGAUUU UUUCUU UCGUU UGCCGCAGACGACUCGCCCGA 0255. _H04
TATTTTCTTCTTTTTCTCCG GGGAGGACGAUGCGGUAUUUUCyUCUUUUUCUCCGCAGACGACUCGCCCGA 0256. _H04
TCCGTCGTCTGTTCGTCCC G6GAGGACGAUGCGGUCCG UCG UCUGUUCGUCCCCAGACGACUCGCCCGA 0257_ _H04
TCTTTTTGTTTCTTTATCCG GGGAGGACGAUGCGGUCUU UUUG UUUCUUUAUCCGCAGACGACUCGCCCGA 025S_ .RQ4
TTTGATTCTCTTTGTTTCCG GGGAGGACGAUGCGGUUUGAUUCUCUUUGUUUCCGCAGACGACUCGCCCGA 0261_ .H04
TCCTGTCGTCTGGTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGGUCGUCCCCAGACGACUCGCCCGA 0252_ _H04
ATTTGTTGTTCTTTTTACCG GGGAGGACGAUGCGGAUUUGUUGUUCUUUUUACCGCAGACGACUCGCCCGA 0265. „HG4
TTCTTCATTTCTCTCTG CCG GGGAGGACGAUGCGGUUCUUCAUUUCUCUCUGCCGCAGACGACUCGCCCGA 0267. .H04
TTTCACTTTTCCATTCTCCG GGGAGGACGAUGCGGU UUCACUUUUCCAU UCUCCGCAGACGACUCGCCCGA 0268_ ,RG4
TCCTTGTCGTCT6TTCGTCCC GGGAGGACGAUGCGGUCCUUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0269. .R04
TTTTAAGTTTTTCGTTTGTG GGGAGGACGAUGCGGUUUUAAGUUUUUCGUUUGUGCAGACGACUCGCCCGA 0270. .H04
GGTTGGAGGTG6TGGATCCG GGGAGGACGAUGCGGGGUUGGAGG UGGUGGAUCCGCAGACGACUCGCCCGA 0271 H04
# in H4
HTS read variable region RNA sequences [election selection Selected sequenc
ACATTCTTCTCTCTTTACCG GGGAGGACGAUGCGGACAU UCUUCUCUCUUUACCGCAGACGACUCGCCCGA 0272 H04
ATTCTCTTTTATCCCTACCG GGGAGGACGAUGCGGAULJCUCUUUUAUCCCUACCGCAGACGACUCGCCCGA 0273_H04
ATTTTCTTTCCTCTTTACCG GGGAGGACGAUGCGGAUUUUCUUUCCUCUUUACCGCAGACGACUCGCCCGA 0275_H04
TTTTTCATACCi'f I I I I CCG GGGAGGACGAUGCGGU UU UUCAUACCU UUUUUCCGCAGACGACUCGCCCGA 0276_H04
TTTTCTTTTCCACTTTTCCG GGGAGGACGAUGCGGU UUUCUUUUCCACUUUUCCGCAGACGACUCGCCCGA 0278_ H04
ATTATCTTTTCTTTGTTCCG GGGAGGACGAUGCGGAUUAUCUUUUCUUUG UUCCGCAGACGACUCGCCCGA 0281_H04
"rTTCTTACTTTTTTCATCCG GGGAGGACGAUGCGGU UUCUUACUUUUUUCAUCCGCAGACGACUCGCCCGA 0282_H04
TTTCTTCTTTCTTCT7TCCG GGGAGGACGAUGCGGU UUCUUCUUUCUUCLJUUCCGCAGACGACUCGCCCGA 0283_H04
TCCTGTCGTCTGTTCGTACC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCG UACCCAGACGACUCGCCCGA G284_HG4
TATTGAGAGAGGAGGTGGTG GGGAGGACGAUGCGGUAUUGAGAGAGGAGGUGGUGCAGACGACUCGCCCGA 0286_ H04
TTTTTGTTATTTTTG CTCTG GGGAGGACGAUGCGGU UUUUGUUAUUUUUGCUCUGCAGAC6ACUCGCCCGA 0288„H04
TTTCTTTTCACTCTTATCCG GGGAGGACGAUGCGGU UUCUUUUCACUCUUAUCCGCAGACGACUCGCCCGA 0290_H04
TCCTGTCGTCTGTTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUUCGUCCCCAGACGACUCGCCCGA 0292_H04
ATGTTTTCTCTTCTTTACCG G6GAGGACGAUGCGGAUGU UUUCUCUUCUU UACCGCAGACGACUCGCCCGA 0293_H04
TATGAAGGGGAGGTGGTCCG GGGAGGACGAUGCGGUAUGAAGGGGAGGUGGUCCGCAGACGACUCGCCCGA 0296_H04
GGTAGGTGTAGGGAGGTCCC 6GGAGGACGAUGCGGGGUAGG UGUAGGGAGGUCCCCAGACGACUCGCCCGA 0299„H04
TTTTATTCACTrTTTGTCCC GGGAGGACGAUGCGGUUUUAU'JCACUUU UUGUCCCCAGACGACUCGCCCGA 030Q_H04
CATC AT GTTTTG CTTTCG TC GGGAGGACGAUGCGGCAUCAUG UUUUGCUUUCGUCCAGACGACUCGCCCGA 0301_H04
CTTTTCTCTTCTTGTTTACCG GGGAGGACGAUGCGGCU UUUCUCU'JCUUGU U UACCGCAGACGACUCGCCCGA G 02_H04
TAπTACTTCTTTTTGCCCG GGGAGGACGAUGCGGUAUU UACUUCUUU UUGCCCGCAGACGACUCGCCCGA 0303_H04
TTTTTGTTCCATTTTATCCG GGGAGGACGAUGCGGUUUUUGUUCCAUUUUAUCCGCAGACGACUCGCCCGA 0304_ HQ4
CTTTTCTTCTTTTCTTTCC G GGGAGGACGAUGCGGCUUUUCUUCUUUUCUU UCCGCAGACGACUCGCCCGA 0306_H04
ATTTTTTCCTGTTTTTGCCG GGGAGGACGAUGCGGAUUUUUUCCUGUUUUUGCCGCAGACGACUCGCCCGA 0307_H04
CTT FATTACTT CTCCG GGGAGGACGAUGCGGCUUUUUAUUACUU UUCUCCGCAGACGACUCGCCCGA 03D8_H04
TTTTTCTTATGTTTG CTCCG GGGAGGACGAUGCGGUUUUUCUUAUGUUUGCUCCGCAGACGACUCGCCCGA 03Q9_HG4
CCTTTTTCTTTCCTTTTCCG GGGAGGACGAUGCGGCCUUUUUCUUUCCUUU UCCGCAGACGACUCGCCCGA 0311_H04
TTTATATTTTGTTTCCTGTG GGGAGGACGAUGCGGUUUAUAU UUUGU UUCCUGUGCAGACGACUCGCCCGA 0312_H04
CTTCTTTTCTCGCTTTTCCG GGGAGGACGAUGCGGCUUCUUUUCUCGCUUU UCCGCAGACGACUCGCCCGA 0313_H04
# !n H4
HIS read variable region RNA sequences tt in H3 selection selection Selected sequenc
ATTTCTCTTCTATTTTACCG GGGAGGACGAUGCGGAUUUCUCUUCUAUU UUACCGCAGACGACUCGCCCGA 0315. _H04
TTCTTTCTTTCCCTTTTCCG GGGAGGACGAUGCGGUUCUUUCUUUCCCUUUUCCGCAGACGACUCGCCCGA 0317. _H04
TTTTCTTGC CCTTTTTTCCG G6GAGGACGAUGCGGU UUUCUUGCCCUUUUUUCCGCAGACGACUCGCCC6A 031S_ .H04
TTTTTCTGTCTTTTGCTCCG GGGAGGACGAUGCGGU UUUUCUG UCUUUUGCUCCGCAGACGACUCGCCCGA 0322. .H04
GATGGTGGTGTAGGGTACTG GGGA6GACGAUGCGGGAUGGUGGUGUAGGGUACUGCAGACGACUCGCCCGA 0324. .H04
TTTTAGCTTCT I i I I i I GTCCG GGGAGGACGAUGCGGUUUUAGCUUCUUUUUUUGUCCGCAGACGACUCGCCCGA 0325. .H04
TTTTCACTATTCTCTCTCCG GGGAGGACGAUGCGGUU UUCACUAU UCUCUCUCCGCAGACGACUCGCCCGA 0326. .H04
CCACTTATTTCCTTTTTCCG GGGAGGACGAUGCGGCCACUUAUUUCCUUU UUCCGCAGACGACUCGCCCGA 0327. .H04
TTCTTTCTTCTTTTC CTCCG GGGAGGACGAUGCGGUUCUUUCUUCUUUUCCUCCGCAGACGACUCGCCCGA 0329. .H04
TTTTATCTTACTTTTTGCTG GGGAGGACGAUGCGGUUUUAUCUUACUUUUUGCUGCAGACGACUCGCCCGA Q330. _H04
AACTACTTTTTCTCTCTCCG GGGAGGACGAUGCGGAACUACUUUUUCUCUCUCCGCAGACGACUCGCCCGA 0331. _H04
TTCTTTTCTTATTCTTTCCG GGGAGGACGAUGCGGUUCU UUUCUUAUUCUUUCCGCAGACGACUCGCCCGA 0332. .H04
TCCTGTCGTCTGTTCGTCCCC GGGAGGACGAUGCGGUCCUG UCG UCUG UUCGUCCCCCAGACGACUCGCCCGA 0334. .H04
CTTTTCATTTCTCTTTACCG GGGAGGACGAUGCGGCUUUUCAUU UCUCUUUACCGCAGACGACUCGCCCGA 0335. .H04
TTTATTTTCTTCTC ATTTC CG GGGAGGACGAUGCGGUUUAUUUUCUUCUCAUUUCCGCAGACGACUCGCCCGA 0337. _H04
ATTCTGTTTTCTTG TTACCG GGGAGGACGAUGCGGAU UCUGUUU UCUUGUUACCGCAGACGACUCGCCCGA 0338. H04
TTTCTTGT TCTTTTCCG GGGAGGACGAUGCGGUU UCUUG UU UCUUUUCCGCAGACGACUCGCCCGA 0339. .H04
πτΊτccττττGCCGπccG GGGAGGACGAUGCGGUU UUUCCUUUUGCCGUUCCGCAGACGACUCGCCCGA 0340. .H04
TTCTTTATCCTTTTTCTCCG GGGAGGACGAUGCGGUUCUUUAUCCUUUUUCUCCGCAGACGACUCGCCCGA 0341. _H04
TCCTGTCGTCTGTTCGTCCT GGGAGGACGAUGCGGUCCUGUCGUCUGUUC6UCCUCAGACGACUCGCCCGA 0342. _H04
TTTATTCTTTTCTTTG CC C C GGGAGGACGAUGCGGUUUAU UCUUUUCUUUGCCCCCAGACGACUCGCCCGA 0344. _H04
TCCTGTCGACTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGACUGUUCGUCCCCAGACGACUCGCCCGA 0345. .H04
CTCATTTCTTCTCTTTTCCG GGGAGGACGAUGCGGCUCAUUUCUUCUCUU UUCCGCAGACGACUCGCCC6A 0346. .H04
CTTTTACTGπCTTTTACCG GGGAGGACGAUGCGGCUUUUACUGUUCUU UUACCGCAGACGACUCGCCCGA 0347. _H04
TTTTTGTCTTGTTT TCCG GGGAGGACGAUGCGGUUUU UGUCUUGUUUUUUCCGCAGACGACUCGCCCGA 0349. _H04
TTTTCTATTATTC1 TCCG GGGAGGACGAUGCGGUUUUCUAUUAUUCUUUUCCGCAGACGACUCGCCCGA 0350. .H04
TCTTTCACTTACTTn'TCCG GGGAGGACGAUGCGGUCUUUCACUUACUUUUUCCGCAGACGACUCGCCCGA 0351. _H04
TTTTCTAG G CTTTTTTTC CG GGGAGGACGAUGCGGUU UUCUAGGCUUUUUUUCCGCAGACGACUCGCCCGA 0352 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTCCAC7TCTTTTCTTTCCG GGGAGGACGAUGCGGUUCCACUUCUUUUCUU UCCGCAGACGACUCGCCCGA 0355. H04
T7TTTGTTTTGTTTTTGGTG GGGAGGACGAUGCGGUU UUUGUUUUGUUUU UGG UGCAGACCACUCGCCCGA 0360. H04
ATTTTA CTCTCATTTTACC G GGGAGGACGAUGCGGAUUUUACUCUCAUUUUACCGCAGACGACUCGCCCGA Q361_ .H04
TCTTTTG CTTTCTTTTCCG GGGAGGACGAUGCGG UCUUUUGCUUUCUUU UCCGCAGACGACUCGCCCGA 0363. _H04
TTTTCATTTACTTCGCTCCG GGGAGGACGAUGCGGUUU UCAUUUACUUCGCUCCGCAGACGACUCGCCCGA 0364. .H04
CCTTTAATTATTTCTCTCCG GGGAGGACGAUGCGGCCUUUAAUUAU UUCUCUCCGCAGACGACUCGCCCGA 0366. .H04
TTTACTCTTTTCACTTTCCC GGGAGGACGAUGCGGU UUACUCUUUUCACUU UCCCCAGACGACUCGCCCGA 0367. _H04
ATTTTCCCTTTCTTTT6CCG GGGAGGACGAUSCGGAUUUUCCCUUUCUUUUGCCGCAGACGACUCGCCCGA 0368. .H04
TTCTTTTCTTTCTTTCTCCG GGGAGGACGAUGCGGU UCUUUUGUUUCUUUCUCCGCAGACGACUCGCCCGA 0370. .H04
AGAGGCGTAGGGAGGTTCCG GGGAGGACGAUGCGGAGAGGCGUAGGGAGGU UCCGCAGACGACUC6CCCGA 0371. .H04
ACCTTTTCTCTCTCTATCCG GGGAGGACGAUGCGGACCUUUUCUCUCUCUAUCCGCAGACGACUCGCCCGA 0372. .H04
TCTTTTTTTGTTTTTCTGCCG GGGAGGACGAUGCGGUCUUUUUUUGUUUUUCUGCCGCAGACGACUCGCCCGA 0374. _H04
CTTTTC ACTCG CTTTTTCCG GGGA6GACGAUGCGGCUUUUCACUC6CUUUUUCCGCAGACGACUCGCCCGA 0375. _H04
TTATTTTATTTCCTTTG CCC GGGAGGACGAUGCGGUUAUUUUAUUUCCUUUGCCCCAGACGACUCGCCCGA 0377. .H04
TCCTGTCGTCTGTGCGTCCC GGGAGGACGAUGGGGUCCUGUCGUCUGUGCGUCCCCAGACGACUCGCCCGA 0378. H04
ATTTTTGTATTCTTTTGCCC GGGAGGACGAUGCGGAUUUUUGUAUUCUUUUGCCCCAGACGACUCeCCCGA 0379. _H04
ATCTATTTATTTTCTCACCG GGGAGGACGAUGCGGAUCUAUUUAUUUUCUCACCGCAGACGACUCGCCCGA 0380. .H04
TTTTCTTTCCTTATTTGCCG GGGAGGACGAUGCGGU UUUCUUUCCUUAUUUGCCGCAGACGACUCGCCCGA 0381. .H04
TTTTTCTCAAGCTTTTTCCG GGGAGGACGAUGCGGU UUU UCUCAAGCUUUU UCCGCAGACGACUCGCCCGA 0382. _H04
CTTCTTTTTCTTTCTTTCCG GGGAGGACGAUGCGGCU UCUUUUUCUUUCUUUCCGCA6ACGACUCGCCCGA 0383. H04
CTTTACCTTTTTCTTTTCCG G6GAGGACGAUGCGGCU UUACCUUUUUCUUUUCCGCAGACGACUCGCCCGA 0384. _H04
TACTTTTC 1 I i l l ! ! CTCCG GGGAGGACGAUGCGGUACU UUUCUUUUU UUCUCCGCAGACGACUCGCCCGA 0385. _H04
TTTCn ATTTCCGTTTTCCG GGGAGGACGAUGCGGUUUCUUAUUUCCGUUUUCCGCAGACGACUCGCCCGA 0387. .H04
CTCTTTCTTCrTTTTCTCCG GGGAGGACGAUGCGGCUCUU UCUUCUUUUUCUCCGCAGACGACUCGCCCGA 0392. .H04
CCTTTTTATTCCTTTTGCCG GGGAGGACGAUGCGGCCUUU UUAUUCCUU UUGCCGCAGACGACUCGCCCGA 0393. .H04
CCTTTTATTATTCCCTTCCG GGGAGGACGAUGCGGCCUUUUAUUAU UCCCUUCCGCAGACGACLICGCCCGA D394. _H04
TTATTCTCTCTTTCTTCCG GGGAGGACGAUGCGGUUAUUCUCUCUUUCUUCCGCAGACGACUCGCCCGA 0397. _H04
TCTTTTT ATTTG CTTTTCCG GGGAGGACGAUGCGGUCUUUUUAUUUGCUU ULCCGCAGACGACUCGCCCGA 0398 H04
# in H4
NTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTACACTTTTCCCCTCCG GGGAGGACGAUGCGGU UUUACACU UUUCCCCUCC6CA6AC6ACUCGCCCGA 0399_ H04
ATTTTTGTCTTTT6TTACTG GGGAGGACGAUGCGGAUUUUUGUCUUUUGUUACUGCAGACGACUCGCCCGA 0400_ H04
TTTTGTT7CACTTTTGTCTG GGGAGGACGAUGCGGU UUUG UUUCACUUUUG UCUGCAGACGACUCGCCCGA 0402_ .H04
TCTCTTTTTC'CTTATCTCCG GGGAGGACGAUGCGGUCUCUUUUUCCUUAUCUCCGCAGACGACUCGCCCGA 0403_ H04
ATATT7TGTTTTTCTTGCCC GGGAGGACGAUGCGGAUAUUU UGUUUUUCUUGCCCCAGACGACUCGCCCGA 0404_ H04
ATTATTTACTCTTTCTACCG GGGAGGACGAUGCGGAUUAUU UACUCUUUCUACCGCAGACGACUCGCCCGA 0406_ .H04
ATCTTTA ATTTCTCTTG C C G GGGAGGACGAUGCGGAUCUUUAAUUUCUCUUGCCGCAGACGACUCGCCCGA Q407_ H04
CTTTTATTCTTG CTCTACCG GGGAGGACGAUGCGGCUUUUAUUCUUGCUCUACCGCAGACGACUCGCCCGA 0408_ H04
CTTTTTTCTCTCTTTTACCG GGGAGGACGAUGCGGCUUUUUUCUCUCU UUUACCGCAGACGACUCGCCCGA 0409_ .H04
CTTTTCCTTTTTTTG TTCCG GGGAGGACGAUGCGGCUUUUCCUU UUUUUG UUCCGCAGACGACUCGCCCGA 0413_ H04
rTTT rcCAACCTTTTTTCG CCCCG GGGAGGACGAUGCGGUUUUUCACUUUUUUCGCCCCGCAGACGACUCGCCCGA 0414„ H04
TTTrcTACTcr "T rccG GGGAGGACGAUGCGGUUUUUCUACUCUUUUUUCCGCA6ACGACUCGCCCGA 0415_ H04
TCCTGTCCTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCCUCUGUUCGUCCCCAGACGACUCGCCCGA 0416_ H04
TCCTGTCGTATGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUAUGU UCG UCCCCAGACGACUCGCCCGA 0417 H04
ACTTTTCTTTCCATTCACCG GGGAGGACGAUGCGGACUUUUCUUUCCAUUCACCGCAGACGACUCGCCCGA 0418_ _H04
GTTGTTTCACTCTTTTTCC6 GGGAGGACGAUGCGGGUUGUUUCACUCUUU UUCCGCAGACGACUCGCCCGA 0420^ H04
ATTTACATTTCTTTTTGCCC GGGAGGACGAUGCGGAUUUACAUUUCUUUU UGCCCCAGACGACUCGCCCGA 0421_ .H04
TCTTTTACATTCCCTTTCCG GGGAGGACGAUGCGGUCUUUUACAUUCCCUUUCCGCAGACGACUCGCCCGA 0426_ .H04
ATCTTTATCTCTTTTCCCCG GGGAGGACGAUGCGGAUCUUUAUCUCUUUUCCCCGCAGACGACUCGCCCGA 0428_ _H04
TTCTTTGTACTTTCCCTtCG GGGAGGACGAUGCGGUUCUUUGUACUUUCCCUCCGCAGACGACLrCGCCCGA 0430_ _H04
GTTTATTCATCCCTTTTCCG GGGAGGACGAUGCGGGUUUAUUCAUCCCUUU UCCGCAGACGACUCGCCCGA 0431_ .H04
ATTTTTCG CCTATTTTACCG GGGAGGACGAUGCGGAUUUUUCGCCUAUUU UACCGCAGACGACUCGCCCGA 0432_ H04
6CTTTTCCTTCTTTTTCCCG GGGAGGACGAUGCGGGCUUUUCCUUCUUUUUCCCGCAGACGACUCGCCCGA 0433. ,H04
ATTCTTGCTTTTTTTTGCCG GGGAGGACGAUGCGGAUUCUUGCUUUUUUUUGCCGCAGACGACUCGCCCGA 0436_ .H04
TTTTTGTCACGTTTTCTCCG GGGAGGACGAUGCGGUUUUUGUCACGUUUUCUCCGCAGACGACUCGCCCGA 043?_ H04
TTTTGTTCTTTTTTCTTCCG GGGAGGACGAUGCGGUUUUG'JUCU UUUUUCUUCCGCAGACGACUCGCCCGA 0439_ .H04
CTTTTATTCACTTTfTTCCG GGGAGGACGAUGCGGCUUUUAUUCACUUUU UUCCGCAGACGACUCGCCCGA 0440_ ,H04
TTATTTTTTTGTTTGTTGTG GGGAGGACGAUGCGGUUAUUUUUUUGUU UGUUGUGCAGACGACUCGCCCGA 0441 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CTTTCTTGTTTGrrCTACCG GGGAGGACGAUGCGGCUUUCUUGUUUCUUCUACCGCACACGACUCGCCCGA 0444_ _H04
TTTTCATTATTTACTTTCCG GGGAGGACGAUGCGGU UUUCAUUAUUUACUUUCCGCAGACGACUCGCCCGA 0445. _H04
ATTTTATTTCCCTTTTACCG GGGAGGACGAUGCGGAUUU UAUUUCCCUUUUACCGCAGACGACUCGCCCGA 0447, .H04
TTTTTGTTAATTTGTCCCTG GGGAGGACGAUGCGGUUUUUGUUAAU UUG UCCCUGCAGACGACUCGCCCGA 0448. _H04
TTTTATCTTTCTTTTTTCCC GGGAGGACGAUGCGGUUUUAUCUUUCUUUU UUCCCCAGACGACUCGCCCGA 0451. _H04
GTTTGCTCTTTTTTCTGCCG G6GAGGACGAUGCGGG U U UGCUCU U U U UUCUGCCGCAG ACGACUCGCCCGA 0452. _H04
TCATTACTTCTTTTCATCCG GGGAGGACGAUGCGGUCAUUACUUCUUUUCAUCCGCAGACGACUCGCCCGA 0453. _H04
TCCGGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCGG UCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0454. _H04
ATTTTCATTTTTTTCCGCCG GGGAGGACGAUGCGGAUUUUCAUUUUUUUCCGCCGCAGACGACUCGCCCGA 0455. _H04
CTTTTTTTATTTCCCTTCCG GGGAGGACGAUGCGGCUUUUUUUAUUUCCCUUCCGCAGACGACUCGCCCGA 0456. _H04
ACTTCTATCCTTCTTTTCCG GGGAGGACGAUGCGGACUUCUAUCCUUCUUUUCCGCAGACGACUCGCCCGA 0458. _H04
TCTTTTCGTTTTCTTCTCCG GGGAGGACGAUGCGGUCUUUUCGU UUUCUUCUCCGCAGACGACUCGCCCGA 0459. _H04
CCGTTTATTTGGTTTTTCCG 6GGAGGACGAUGCGGCCGUU UAUU UCCUUUUUCCGCAGACGACUCGCCCGA 0460. _H04
TA7TT ATTTTTTCTTG C C CCG GGGAGGACGAUGCGGUAUUUAUUUUUUCUUGCCCCGCAGACGACUCGCCCGA 0461. H04
TTTTCTCACTCTTTTCTCCG GGGAGGACGAUGCGGUUUUCUCACUCUUUUCUCCGCAGACGACUCGCCCGA 0462. H04
ATTTCTTATTCCTTTCCCCG GGGAGGACGAUGCGGAUUUCUUAU UCCUUUCCCCGCAGACGACUCGCCCGA 0463. _H04
TTATTTTCA CTGTTTTTG CC GGGAGGACGAUGCGGU UAUUUUCACUGUU UUUGCCCAGACGACUCGCCCGA 0464. _H04
TTTTTCACTTTTTCTTCTGCGC GGGAGGACGAUGCGGUUUUUCACU UUUUCUUCU6CGCCAGACGACUCGCCCGA 0465. H04
TTTTTTATTTTCCTG G TTC CG GGGAGGACGAUGCGGU UUUUUAUUUUCCUG6UUCC6CAGAC6ACUCGCCCGA 0466. H04
TCTATTTCTT'GCTTTTTCCG GGGAGGACGAUGCGGUCUAUU UCUUGCUUUU UCCGCAGACGACUCGCCCGA 0467. H04
TTCTTTA I M i l I ! CCCTCCG GGGAGGACGAUGCGGU UCUUUAUU UUUUUCCCLCCGCAGACGACUCGCCCGA 0468. _H04
TCGTTTATTTCi I I I ! I CCG GGGAGGACGAUGCGGUCGU UUAUUUCUU UU UUCCGCAGACGACUCGCCCGA 0469. .H04
rCCTGTCGTC TACGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUUACGUCCCCAGACGACUCGCCCGA 0470. HQ4
ATCTTTCTTCTCTATTACCG GGGAGGACGAUGCGGAUCUUUCUUCUCUAUUACCGCAGACGACUCGCCCGA 0471. _H04
ATTTG TTATGTTTTG TACC G GGGAGGACGAUGCGGAUUUGUUAUGUUUUGUACCGCAGACGACUCGCCCGA 0472. _H04
ITITTGTTTTTATTTTTCCG GGGAGGACGAUGCGGUUUU UGUU UUUAUU UU UCCGCAGACGACUCGCCCGA 0473. H04
TTTTTTCCTCGCTTTTGCCG GGGAGGACGAUGCGGUUUU UUCCUCGCUUUUGCCGCAGACGACUCGCCCGA 0475. H04
TTCG CTTTCTCTTTTATCCG GGGAGGACGAUGCGGUUCGCUUUCUCUUUUAUCCGCAGACGACUCGCCCGA 0476. _H04
n in
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTCTTGATCCTTTTTTCCG GGGA6GACGAUGCGGUUUCUUGAUCCUUUU UUCCGCAGAC6ACUCGCCCGA 0478. _H04
ATTTTCTCACTCTCTTACCG GGGAGGACGAUGCGGAUUU UCUCACUCUCUUACCGCAGACGACUCGCCCGA 0479, _H04
TTTTTGTTATTCTTTTCCG GGGAGGACGAUGCGGUUUUUCUUAUUCUUUUCCGCAGACGACUCGCCCGA 0481_ _H04
TCCTGTCGTCTGTTCCCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCCCCCCAGACGACUCGCeCGA 0482. H04
ATTTATCTTCTTTTTCCCCG GGGAGGACGAUGCGGAUUUAUCUUCUUU IIUCCCCGCAGACGACUCGCCCGA 0483. H04
CTTTTTCTTCATT rCCG GGGAGGACGAUGCGGCUUUUUCUUCAUUUU UUCCGCAGACGACUCGCCCGA 0484. H04
CTTAcn TTACTTCCTCCG G6GA6GACGAUGCGGCUUACUUUUUACUUCCUCCGCAGACGACUCGCCCGA 0485, .H04
TTTTCAATTATTTCTGTCCG GGGAGGACGAUGCGGU UUUCAAUUAUUUCUG UCCGCAGACGACUCGCCCGA 0486. .H04
TTTCTCTTCTTTTCTATCCG GGGAGGACGAUGCGGUUUCUCUUCUUUUCUAUCCGCAGACGACUCGCCCGA 0487. .H04
ATCTTTTCGCTTT7TTACCG GGGAGGACGAUGCGGAUGUUUUCGCUUUUUUACCGCAGACGACUCGCCCGA 0488. _H04
TCCTG TCGTCG GTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCGGUUCG UCCCGAGACGACUCGCCCGA 0489. .H04
TCTCATCTTCTTTTTCTCCG GGGAGGACGAUGCGGUCUCAUCUUCUUUU UCUCCGCAGACGACUCGCCCGA 0490. .H04
TTCTTGCTTTATTmTCCG GGGAGGACGAUGCGGU UCUUGCUUUAUUUU UUCCGCAGACGACUCGCCCGA 0491. .H04
ATTTTATTGTTTTTTTA CG GGGAGGACGAUGCGGAUUU UAUUGUUUUUUUACCGCAGACGACUCGCCCGA 0492. _H04
TTTTTACCC I I I 1 i ! I GTCCG GGGAGGACGAUGCGGUUUU UACCCUUUUUUUGUCCGCAGACGACUCGCCCGA 0493. _H04
TT7TTTCAGATTTGTTTCGG GGGAGGACGAUGCGGUUUU UUCAGAUUUGUUUCCGCAGACGACUCGCCCGA 0495. .H04
TCTCπCTTTCTTTATCGCCG GGGAGGACGAUGCGGUCUCUUCUUUCUUUAUCGCCGCAGACGACUCGCCCGA 0496. .H04
CTTTTCTTTTTCCCTATCCG GGGAGGACGAUGCGGCU UUUCU UU UUCCCUAUCCGCAGACGACUCGCCCGA 0497. -H04
CCATTCAGTTTTCTTTTCCG GGGAGGACGAUGCGGCCAUUCACUUUUCUUUUCCGCAGACGACUCGCCCGA 0498. ,H04
TTTTCTCTTTTCCATTTCCG GGGAGGACGAUGCG6UUUUCUCUUUUCCAUU UCCGCAGACGACUCGCCCGA 0499. . H0
CTTTTCTCTTTTTTCCG CGC GGGAGGACGAUGCGGCUUU UCUCUUUUUUCCGCGCCAGACGACUCGCCCGA 0500. _H04
TTTGTATTCCCCTTTTTCCG GGGAGGACGAUGCGGUUUGUAUUCCCCUUUU UCCGCAGACGACUCGCCCGA 0S02. H04
TCTCTTTATTTTTTCTTCCG GGGAGGACGAUGCGGUCUCU UUAUUUUU UCU IJCCGCAGACGACUCGCCCGA 0503. .H04
ATTCTCTTTTTTTTCCCACCG GGGAGGACGAUGCGGAU UCUCU UUUUUU UCCCACCGCAGACGACUCGCCCGA 0506. .H04
CTTTCTACTCTCTTCTTCCG GGGAGGACGAUGCGGCUUUCUACUCUCUUCUUCCGCAGACGACUCGCCCGA 0507. .H04
GTTGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGGUUGGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 0508. H04
T TTATTATTACCTGTTCCG GGGAGGACGAUGC6GU UUUAUUAUUACCUGU UCCGCAGACGACUCGCCCGA 0509. .H04
ATTTTCTCATCCTTTTG CCG GGGAGGACGAUGCGGAU UUUCUCAUCCUU UUGCCGCAGACGACUCGCCCGA 0520 H04
# in H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc
TACATTCTTTTTTCTCTCCG GGGAGGACGAUGCGGUACAUUCULJUU UUCUCUCCGCAGACGACUCGCCCGA 0511. _HG4
TTTGGGAGTAGGGTGTCCG GGGAGGACGAUGCGGU UUGGGAG UAGGGUGUCCGCAGACGACUCGCCCGA 0512. .H04
TTCATTTACATTCTTTTCCG GGGAGGACGAUGCGGU UCAUUUACAUUCUUUUCCGCAGACGACUCGCCCGA 0513. _H04
ACTTTCA CTATT !TACCG GGGAGGACGAUGCGGACUUUCACUAUUUUUUACCGCAGACGACUCGCCCGA 0515. . H04
ATCATG TCTTCT rcTccG GGGAGGACGAUGCGGAUCAUGUCUUCUUU UCUCCGCAGACGACUCGCCCGA 051G. .H04
A ACTACT 1" ! "Γ 'Π' CTCTCTCCG GGGAGGACGAUGCGGAACUACUUUUUUCUCUCUCCGCAGACGACUCGCCCGA 0517_ _H04
TTTCCTTlTTTiCCCATCCG GGGAGGACGAUGCGGUUUCCUUUUUUUCCCAUCCGCAGACGACUCGCCCGA 0520. .H04
TTTCTTACTCTTCATTTCCG GGGAGGACGAUGCGGUUUCUUACUCUUCAUUUCCGCAGACGACUCGCCCGA 0521. .H04
TCTTCATTCTATTTTCTCCG GGGAGGACGAUGCGGUCUUCAUUCUAUUUUCUCCGCAGACGACUCGCCCGA 0522. .H04
TTTTCTTAATCCCTTTTCCG GGGAGGACGAUGCGGUUUUCUUAAUCCCUUUUCCGCAGAC6ACUCGCCCGA 0523. _H04
ATTGrmATTGrnTACcG GGGAGGACGAUGCGGAUUGUUUUAUUGUUUUACCGCAGACGACUCGCCCGA 0524. _H04
GATTTCTATTTTCTCCACCC GGGAGGACGAUGCGGGAUUUCUAUUUUCUCCACCCCAGACGACUCGCCCGA 0526. H04
ATTTTTGTTCTTGTCTACCG GGGAGGACGAUGCGGAU UUUUGUUCUUG UCUACCGCAGACGACUCGCCCGA 0527. .H04
GT TAT TTACCTTCTCCG GGGAGGACGAUGCGGCUUU UAUUUUACCUUCUCCGCAGACGACUCGCCCGA 0528. .H04
TTTTTCATTTTACCGTTCCG GGGAGGACGAUGCGGUU UUUCAUU UUACCGUUCCGCAGACGACUCGCCCGA 0529. .H04
TTTTTCTCTTTTTTCCG CCG GGGAGGACGAUGCGGUUUUUCUCUUUUUUCCGCCGCAGACGACUCGCCCGA 0531. .H04
TCTCTTACAATTTTCTTCCG GGGAGGACGAUGCGGUCUCU UACAAUUUUCUUCCGCAGACGACUCGCCCGA 0533. .H04
T6TTFCTCTTACTTTTTCCG GGGAGGACGAUGCGGUGUU UCUCUUACUUUUUCCGCAGACGACUCGCCCGA 0534. .HQ4
TCTTTTATGAATTCTCTCCG GG6A6GACGAUGCGGUCUUUUAUCAAUUCUCUCCGCAGACGACUCGCCCGA 0536. .H04
AATT ATG TG TTTTT6TG A C CG GGGAGGACGAUGCGGAAUUAUGUGUUUUUGUGACCGCAGACGACUCGCCCGA 0537. .H04
TCGGTGGTGTGGGGATTCCG GGGAGGACGAUGCGGUCGGUGGUGUGGGGAU UCCGCAGACGACUCGCCCGA 0538. _H04
ACCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGACCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0539. _H04
ACTT'i A'lTCTCTTTTTTCCG GG6AGGACGAUGCGGACUUUAUUCUCUUUUUUCCGCAGACGACUCGCCCGA 0543. .H04
TTATTTGTTTATTTTCTCCG GGGAGGACGAUGCGGUUAU UUGUU UAUUUUCUCCGCAGACGACUCGCCCGA 0544. .H04
TTTATTTAT7TTTCCGTCCC GGGAGGACGAUGCGGUUUAUUUAU UUUUCCGUCCCCAGACGACUCGCCCGA 0545. _H04
TTCTTTTTACTTTCCTTCCG GGGAGGACGAUGCGGUUCUU UUUACUUUCCUUCCGCAGACGACUCGCCCGA 0546. .H04
TATTTTTCCTTTTCCATGTG GGGAGGACGAUGCGGUAUU UUUCCUUUUCCAUGUGCAGAGGACUCGCCCGA 0547. .H04
CTTTTCTTTGTTGCTTTCCG GGGAGGACGAUGCGGCUUUUCUUUGUUGCUUUCCGCAGACGACUCGCCCGA 0548 H04
# in H4
NTS read variable region RNA sequences selection selection Selected sequenc
AGTTGTTTTCCTTTTGTCCG GGGAGGACGAUGCGGAGUUGUUUUCCUU UUCUCCCCAGACGACUCGCCCGA 0549JH04
AATTTG CTTCTTTTTATCCG GGGAGGACGAUGCGGAAUUUGCUUCUUUUUAUCCGCAGACGACUCGCCCGA 0550_HQ4
CTTTG ACTTACTTTCTACC G GGGAGGACGAUGCGGCU UUCACUUACUUUCUACCGCAGACGACUCGCCCGA 0551_H04
GTTCTTTATTCTTCTCACCG GGGAGGACGAUGCGGGUUCUUUAUUCUUCUCACCGCAGACGAGUCGCCCGA 0552„H04
A I M I I I ACTTCTTTCACCG GGGAGGACGAUGCGGAUUU UUIJACUUCUUUCACCGCAGACGACUCGCCCGA 0553_HC4
TCCTGTGGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUGGUCUGUUCG UCCCCAGACGACUCGCCCGA 0554_H04
TTTCTCTACACTTTTTGCCG GGGAGGACGAUGCGGUUUCUCUACACUUUUUGCCGCAGACGACUCGCCCGA 0555„H04
CTTACTCATTTCTCTCTCCG GGGAGGACGAUGGGGCUUACUCAUUUCUCUCUCCGCAGACGACUCGCCCGA 0556_H04
ATTTCCTATCCTTTTTACCG GGGAGGACGAUGCGGAU UUCCUAUCCUUU UUACCGCAGACGACUCGCCCGA 0557JH04
CTCTTTACTTTTCTCCTCCG GGGAGGACGAUGCGGCUCUU UACUUU UCUCCUCCGCAGACGACUCGCCCGA 0558JH04
ATTTTCTATTTATTC CACCG G6GAGGACGAUGC6GAUUU UCUAUUUAUUCCACCGCAGACGACUCGCCCGA 0559JH04
TTGTT ATTCTTTTTTTG CCC GGGAGGACGAUGCGGUUGU UAUUCUU UUUUUGCCCCAGACGACUCGCCCGA OS60_H04
GTTTCCTTTCTCTTTTCCCG GGGAGGACGAUGCGGGUUUCCU UUCUCUUUUCCCGCAGACGACUCGCCCGA 0562JH04
TTTCATTATTACTTTCTGCC GGGAGGACGAUGCGGUUUCAUUAUUACUUUCUGCCCAGACGACUCGCCCGA 0563_H04
CTTTTTACCTTCTAT7TCCG GGGAGGACGAUGCGGCUUU UUACCUUCUAUUUCCGCAGACGACUCGCCCGA 0564J-K34
TCCTCTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCliCUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0565_H04
CTTTTACCTTTCTTTATCCG GGGAGGACGAUGCGGCUUU UACCUU UCUUUAUCCGCAGACGACUCGCCCGA 0S66_H04
TTTTCCTTATTCTTTTTCCG GGGAGGACGAUGCGGUU UUCCUUAUUCUU UUUCCGCAGACGACUCGCCCGA 0S67_H04
TTATTTTTCTTCGCTGTCCG GGGAGGACGAUGCGGUUAUUUUUCUUCGCUG UCCGCAGACGACUCGCCCGA 0568_H04
TCI I I I I TiCACTTTCTCCG GGGAGGACGAUGCGGUCUUUUU UUCACUU UCUCCGCAGACGACUCGCCCGA 0S69_H04
GTTTTACATTTCTTTCCCCC GGGAGGACGAUGCGGGUUU UACAUUUCUUUCCCCCCAGACGACUCGCCCGA 0570„H04
TTTCTG AC ACTTTTTTTCCG GGGAGGACGAUGCGGUUUCUGACACUUUUUUUCCGCAGACGACUCGCCCGA OS71_H04
TTTCTTTTTCTTCTTCTCTG GGGAGGACGAUGCGGUUUCU UU UUCUUCUUCUCUGCAGACGACUCGCCCGA 0572_ H04
TTTGGGAGTAGGGTGGTACCG 6GGAGGACGAUGCGGUUUGGGAGUA6GGUG6UACCGCAGACGACUCGCCCGA 0573_H04
GTTATTTCTCTCTTTGTCCG GGGAGGACGAUGCGGGUUAUUUCUCUCUUUGUCCGCAGACGACUCGCCCGA 057 _H04
TTTTTCTCTTTACGTTTCCG GGGAGGACGAUGCGGUUUU UCUCUUUACG UU UCCGCAGACGACUCGCCCGA 0575_HQ4
ΤΤΤ6π6ΤΤΑΤΤ6ΤΤΰΚ€6 GGGAGGAGGAUGCGGUUUGUUGUUAUUGUUGUCCGCAGACGACUCGCCCGA 0576_H04
TCTATTTCCTTTCTTTTCCG GGGAGGACGAUGCGGUCUAU UUCCUUUCUUUUCCGCAGACGACUCGCCCGA QS7 _ H04
Figure imgf000174_0001
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTCTTATATCTCTGTCCG GGGAGGACGAUGCGGU UUUCUUAUAUCUCUG UCCGCAGACGACUCGCCCGA 0578. _H04
GATTTTGTCTCTT7TACCCG GGGAGGACGAUGCGGGAUU UUGUCUCUUUUACCCGCAGACGACUCGCCCGA 0579. _H04
TTCGTTATTTCCTTTTTCCG GGGAGGACGAUGCGGU UCGUUAUUUCCUUU UUCCGCAGACGACUCGCCCGA 058G„ _H04
TTTTTCCTACCT ITTCCG GGGAGGACGAUGCGGU UUUUCCUACCUUUUUUCCGCAGACGACUCGCCCGA 0581. .H04
CTTTGTTTTACTGTTTTCCG GGGAGGACGAUGCGGCUUUGUUUUACUGUUUUCCGCAGACGACUCGCCCGA 0583. .H04
τcτττAπ TTCCTTTCCG GGGAGGACGAUGCGGUCUUUAUUUUUUCCUUUCCGCAGACGACUCGCCCGA 0584_ _H04
TGT" GTTTATTTTG CCG GGGAGGACGAUGCGGUGUUUUUG UUUAUUUUGCCGCAGACGACUCGCCCGA 0585, „H04
GTTATTCTTCTCTATTTCCG GGGAGGACGAUGCGGG UUAUUCU UCUCUAUUUCCGCAGACGACUCGCCCGA 0586. H04
CTACTTCTTATTTCTTTCCG GGGAGGACGAUGCGGCUACUUCUUAUUUCUUUCCGCAGACGACUCGCCCGA 0588. .H04
TTTTTCATTTCCTTTTCCCG GGGAGGACGAUGCGGU UUUUCAUUUCCUUUUCCCGCAGACGACUCGC CGA 0589. H04
TATCTTTT6CTTTCCCTCC6 GGGAGGACGAUGCGGUAUCUUUUGCUUUCCCUCCGCAGACGACUCGCCCGA 0591. _H04
TTATTATCTTTCTCG CTCCG GGGAGGACGAUGCGGUUAUUAUCUUl!CUCGCUCCGCAGACGACUCGCCCGA 0592, _H04
TTCTTATTCCTTCTATTCCG GGGAGGACGAUGCGGUUCUUAUUCCUUCUAUUCCGCAGACGACUCGCCCGA 0593_ _H04
CCTTTTTACTCTCTCTTCCG GGGAGGACGAUGCGGCCUUUUUACUCUCUCUUCCGCAGACGACUCGCCCGA 0594 _H04
TCACTTACTTTTTTCCTCTG GGGAGGACGAUGCGGUCAaJUACUUUUUUCCUCUGCAGACGACUCGCCCGA 0595., , H04
TTACTTATTTGCTTTTTCCC GGGAGGACGAUGCGGUUACUUAUUUGCUUUUUCCCCAGACGACUCGCCCGA 0597. _H04
TTTGTCAACTTTCTTTTCCG GGGAGGACGAUGCGGUUUGUCAACUUUCUUUUCCGCAGACGACUCGCCCGA 0598. _H04
ATTTTTGG TATTTTTT ACCG GGGAGGACGAUGCGGAUUU UUGGUAU UUUUUACCGCAGACGACUCGCCCGA 0599. .H04
TTTATTCTG H i l l ni'CCG GGGAGGACGAUGCGGU UUAUUCUGUUUU UU UUCCGCAGACGACUCGCCCGA 0600. _H04
ACTCACGTTli'CTTCTTGCG GGGAGGACGAUGCGGACUCACGU UUUCUUCUUCCGCAGACGACUCGCCCGA 0601_ _H04
TTACTTTCTTCTCTTTTCCC GGGAGGACGAUGCGGUUACUUUCUUCUCUUUUCCCCAGACGACUCGCCCGA 0602. .H04
TTTGTTTTATTTTfTTGTCCC GGGAGGACGAUGCGGUUUGUUUUAU UUUUUUGUCCCCAGACGACUCGCCCGA 06Q3_ _H04
TTTCT TATTTCCCCTG GGGAGGACGAUGCGGUUUCUUUUUUAUUUCCCCUGCAGACGACUCGCCCGA 0605. .H04
TGAGTCAC I I I I i i ACCCC GGGAGGACGAUGCGGUGAGUCACAUUUUUUACCCCCAGACGACUCGCCCGA 0605. .H04
TTTGGGAGTAGGGGGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGGG UUCCGCAGACGACUCGCCCGA 0607. _H04
ATTTCTCACTTCTTTTACCG GGGAGGACGAUGCGGAUUUCUCACUUCUUUUACCGCAGACGACUCGCCCGA 0608. JH04
CTTTCTTTTCC TTATTTCCG GGGAGGACGAUGCGGCUUUCUU UUCCUUAUUUCCGCAGACGACUCGCCCGA 0609. H04
I I 1 1 i I CACGGTTTTTTCTG GGGAGGACGAUGCGGUUUU UUCACGGUUUUU UCUGCAGACGACUCGCCCGA 0610 H04
# m H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTGTCTTTTCCTTTTCCG GGGAGGACGAUGCGGUUUUG UCUUUUCCUU JUCCGCAGACGACUCGCCCCA 0613. _H04
ATTTTCTTTCTTCTTTTCCG GGGAGGACGAUGCGGAUUUUCUU UCUUCUUUUCCGCAGACGACUCGCCCGA 0614. „H04
TTTA I ί i ΠΊ CCCGTTTGTG GGGAGGACGAUGCGGUUUAUUUUUUCCCGU UUGUGCAGACGACUCGCCCGA 0615. .H04
TCTTTTTTGTCTTTCTTCCG GGGAGGACGAUGCGGUCUUUU UUG UCU UUCUUCCGCAGACGACUCGCCCGA 0617. .H04
TTTATTCATTTCTTGTTCCC GGGAGGACGAUGCGGUUUAUUCAUU UCUUGUUCCCCAGACGACUCGCCCGA 0620. .H04
ATTTGTTATTTTTG TTTG CC GGGAGGACGAUGCGGAUU UGUUAUUUUUGUUUGCCCAGACGACUCGCCCGA 0621. „HG4
G CCTGTCGTCTGTTC GTCCC GGGAGGACGAUGCGGGCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0622. „HQ4
TCTTTTCATTTTTCGTTCCG GGGAGGACGAUGCGGUCUU UUCAUUUUUCGUUCCGCAGACGACUCGCCCGA 0524. .H04
TTTTCCG TTCCTTTTTTCCG GGGAGGACGAUGCGGU UUUCCGUUCCUUUUUUCCGCAGACGACUCGCCCGA 0625. _H04
CTTTTTCTTTTCTCTTTCCG GGGAGGACGAUGCGGCUUUUUCUUUUCUCUUUCC6CAGACGACUCGCCCGA 0626. _H04
TTCG CTTATCTTTATTTCCG GGGAGGACGAUGCGGU UCGCUUAUCUUUAUU UCCGCAGACGACUCGCCCGA 0628. _H04
TATTTTTATTTTCTCCGCCG GGGAGGACGAUGCGGUAUUUU UAUUUUCUCCGCCGCAGACGACUCGCCCGA 0630. .H04
CTTCTATCTTTTCTCTACCG GGGAGGACGAUGCGGCUUCUAUCUUUUCUCUACCGCAGACGACUCGCCCGA 0631. H04
GTTACTTTCTTTCTTTTCCG GGGAGGACGAUGCGGGUUACUUUCUUUCUU UUCCGCAGACGACUCGCCCGA 0632. _H04
TTTTATTTCTTTTTTCCCCC GGGAGGACGAUGCGGUUUUAUUUCUUU UUUCCCCCCAGACGACUCGCGCGA 0633. .H04
AGTTTTTGTCTTTTTTCCCC GGGAGGACGAUGCGGAGUUUUUG UCUUUUUUCCCCCAGACGACUCGCCCGA 0634. _HQ4
ATTTTCTC I I ! ! I i iCTGCCG GGGAGGACGAUGCGGAUUU JCUCUUUUUUUCUGCCGCAGACGACUCGCCCGA 0635. .H04
imACATTCATTTTTGCGC GGGAGGACGAUGCGGUUU UACAUUCAU UUUUGCGCCAGACGACUCGCCCGA 0636. H04
CCATTCTTTTATTTTCTCCG GGGAGGACGAUGCGGCCAUUCUUUUAUUUUCUCCGCAGACGACUCGCCCGA 0637. .H04
TTTTCATTC I ! I I 1 I i CC CC GGGAGGACGAUGCGGUUUUCAUUCUUUUUUUCCCCCAGACGACUCGCCCGA 0638. .H04
TGTTTTATTTGTl'TTTTCCC GGGAGGACGAUGCGGUGUUUUAUUUGUUUUU UCCCCAGACGACUCGCCCGA 0639. H04
TTTTCTTCTTG CTTCTTC CG GGGAGGACGAUGCGGUUUUCU UCU UGCUUCUUCCGCAGACGACUCGCCC6A 0640. .H04
ATTTTTTG TTGTTTTG ACCG GGGAGGACGAUGCGGAUUUUUUGUUGUULiUGACCGCAGACGACUCGCCCGA 0641. .H04
TTTCACTC I 1 HTI CCCCGC GGGAGGACGAUGCGGUUUCACUCUU UUUUCCCCGCCAGACGACUCGCCCGA 0643. H04
AATTCTTTATCTTTCCACCG GGGAGGACGAUGCGGAAUUCUU UAUCUUUCCACCGCAGACGACUCGCCCGA 0645. _H04
ATTCACTCTTTTCCTTGCCG GCGAGGACGAU'GCGGAUUCACUCU UUUCCUUGCCGCAGACGACUCGCCCGA 0646. _H04
CAGTTTTACTTTTTCTTTGCC GGGAGGACGAUGCGGCAGL UUUACUUU UUCU UUGCCCAGACGACUCGCCCGA 0647. .H04
AATT G C TCTTTTTTTCT ACC G GGGAGGACGAUGCGGAAUUGCUCUUUUUUUCUAeCGCAGACGACUCGCCCGA 0648. .H04
# in H4
HTS read variable region RNA sequences election selection Selected sequenc
CATT TATTATTTCTCTCCG GGGAGGACGAUGCGGCAUU UUAUUAUUUCUCUCCGCAGACGACUCGCCCGA 0650_H04
AC I I M I i l CTTTTCTACCG GGGAGGACGAUGCGGACUU UUUUUCUUU UCUACCGCAGACGACUCGCCCGA 0651_H04
TTTCTTTTCATTTTTTTCCG GGGAGGACGAUGCGGUUUCUUUUCAUUU UUUUCCGCAGACGACUCGCCCGA 0652_H04
CTCTTTTTTCCCTTTCTCCG GGGAGGACGAUGCGGCUCUUUUUUCCCUUUCUCCGCAGACGACUCGCCCGA O653_H04
CTTiACTTTTTATTTTTCCG GGGAGGACGAUGCGGCUUUACUU UUUAU UUU UCCGCAGACGACUCGCCCGA 0654_ H04
TCCTGTCGTCAGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCAGUUCGUCCCCAGACGACUCGCCCGA 0655_H04
TTTATCTTTAACTTTTTCCG GGGAGCACGAUGCGGU UUAUCUUUAACUUUU UCCGCAGACGACUCGCCCGA 0656JH04
TTTTCT ATCTTTTTTGTC CG GG GAGG ACGA UGCG GU UUUCUAUCUUU U UUG UCCGCAGACG ACU CGCCCG A Q657JH04
GTTCTTGTTTCCTTTTACCG GGGAGGACGAUGeGGGUUCUUGUUUCCUUUUACCGCAGACGACUCGCCCGA 06S8_H04
CTTTTCC M i l l ! CACTCCG GGGAGGACGAUGCGGCUUUUCCUUUUUUCACUCCGCAGACGACUCGCCCGA G66Q_H04
TCTACTATTTTCTCCATCCG GGGAGGACGAUGCGGUCUACUAUUUUCUCCAUCCGCAGACGACUCGCCCGA 0661_H04
TTTG CTTT AT ATTTTG TCC C GGGAGGACGAUGCGGU UUGCUUUAUAUU UUGUCCCCAGACGACUCGCCCGA 0662_H04
TCCTGTCG TCTG TTCTC CC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCUCCCCAGACGACUCGCCCGA 0663_H04
CCTTATTGTTCTTGTTTCCG GGGAGGACGAUGCGGCCUUAUUGUUCUUGUU UCCGCAGACGACUCGCCCGA 0δ64_Η04
TATTTACTTTCTTTCCTCCG GGGAGGACGAUGCGGUAUUUACUUUCUUUCCUCCGCAGACGACUCGCCCGA 0665_H04
TCTCTTATGTATTCCTCCG GGGAGGACGAUGCGGUCUCU UAUGUAUUCUCUCCGCAGACGACUCGCCCGA 066&_H04
TTT'tTTAGTCTATTTTTCCG GGGAGGACGAUGCGGU UUU UUAGUCUAU UUUUCCGCAGACGACUCGCCCGA G667_H04
TGAAGGAGGAGGAGGGACTG GGGAGGACGAUGCGGUGAAGGAGGAGGAGGGACUGCAGACGACUCGCCCGA 0669_H04
TTiTCACTATATTTTCTCCG GGGAGGACGAUGCGGUUUUCACUAUAUUUUCUCCGCAGACGACUCGCCCGA 0670_HQ4
ATTCTCTTTTTTTCCCACCG GGGAGGACGAUGCGGAUUCUCUUUUUUUCCCACCGCAGACGACUCGCCCGA 0671_H04
TTTTTCTCACAATTCTCATCCG GGGAGGACGAUGCG6UUUU UCUCACAAUUCUCAUCCGCAGACGACUCGCCCGA 0672_H04
ATTCTTTGATTCTTTTTCCG GGGAGGACGAUGCGGAUUCUUUGAUUCUUUUUCCGCAGACGACUCGCCCGA 0674_H04
TTTCCACGGTTTTTTTTCCG GGGAGGACGAUGCGGUUUCCACGGUUUUU UUUCCGCAGACGA.CUCGCCCGA 0675_H04
CTTATTTATTTTTCTCTCCG GGGAGGACGAUGCGGCUUAUUUAUUUUUCUCUCCGCAGACGACUCGCCCGA 06 6_H04
TTTGTTTTCTTGATTTTCCC GGGAGGACGAUGCGGUUUGUUU UCU UGAUU UUCCCCAGACGACUCGCCCGA 0677_H04
AATTATGTGTTTTTTTGTGCCG GGGAGGACGAUGCGGAAUUAUGUGUUUU UUUG UGCCGCAGACGACUCGCCCGA 0678 H04
CTTTCTCTTCTCTTCTTCCG GGGAGGACGAUGCGGCUUUCUCUUCUCUUCUUCCGCAGACGACUCGC.CCGA 0679„K04
CTTTCTTTGTTTCCTCTCCG GGGAGGACGAUGCGGCUUUCUUU6UUUCCUCUCCGCAGACGACUCGCCCGA 0680 H04
# in H4
HTS read variable region RNA sequences election selection Selected sequenc
TCTTTTAACACCTTTTTCCG GGGAGGACGAUGCGGUCUUUUAACACCUUUUUCCGCAGACGACUCGCCCGA 0681_H04
TTGTTTCTACCTTI I I i CCG GGGAGGACGAUGCGGUUGUUUCUACCUUUUUUCCGCAGACGACUCGCCCGA 0683JH04
TCTGTTTTACTTTTTTTCCC GGGAGGACGAUGCGG'JCUG UUU UACUUUUU UUCCCCAGACGACUCGCCCGA 0684 HQ4
—
TTTTATACTGTTTTTCCCGC GGGAGGACGAUGCGGUUUUAUACUGUUUUUCCCGCCAGACGACUCGCCCGA 06S5„H04
C'FTTTTCAACTTCTTTTCCG GGGAGGACGAUGCGGCUUUUUCAACUUCUUUUCCGCAGACGACUCGCCCGA 0686„H04
ATTTTTCTCCTTTTTCACCG GGGAGGACGAUGCGGAUUULJUCUCCU UUUUCA tGCAGACGACUCGCCCGA Q687_H04
TCTTTATATCACTTTTTCCG GGGAGGACGAUGCGGUCUUUAUAUCACUUUU UCCGCAGACGACUCGCCCGA 0688__H04
TTTCTAATCTCCTTTTTCCG GGGAGGACGAUGCGGU UUCUAAUCUCCUUUUUCCGCAGACGACUCGCCCGA 0689_H04
TACTTCTTTTTTTCTCTCCG GGGAGGACGAUGCGGUACUUCU UUUUUUCUCUCCGCAGACGACUCGCCCGA 0690_H04
ACCTTATCTCCTTTTCTCCG GGGAGGACGAUGCGGACCUUAUCUCCUUUUCUCCGCAGACGACUCGCCCGA 0691_H04
ATCTTTTCCCGCTTTTTCCG GGGAGGACGAUGCGGAUCUUUUCCCGCU UUUUCCGCAGACGACUCGCCCGA 0692_ HG4
TTTTTCCATATTCTCTGTCCG GGGAGGACGAUGCGGUUU UUCCAUAUUCUCUGJCCGCAGACGACUCGCCCGA 0693_H04
TTTG GGAGTAG G GCGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGCG UtJCCGCAGACGACUCGCCCGA 0694_H04
ATCTTTTTGTTTTTCCTCCG GGGAGGACGA'UGCGGAUCUUUUUGUUUUUCCUCCGCAGACGACUCGCCCGA 0695_H04
TCTCTTTTCTTTCTTATCCG GGGAGGACGAUGCGGUCUCU UUUCUUUCUUAUCCGCAGACGACUCGCCCGA 0702 JH04
ACTTCTTTATTTTTCTTCCG GGGAGGACGAUGCGGACULCUUUAUU UUUCUUCCGCAGACGACUCGCCCGA 0703 JH04
CATTCTTATCTCTTTCTCCG GGGAGGACGAUGCGGCAUUCUUAUCUCUUUCUCCGCAGACGACUCGCCCGA 07G6JH04
AATTCCTTTCTTCTTTACCG GGGAGGACGAUGCGGAAUUCCUUUCUUCUUUACCGCAGACGACUCGCCCGA 0 07 JH 04
TCCTTGTCGTCTTTCGTCCC GGGAGGACGAUGCGGUCGUUGUCGUCUUUCGUCCCCAGACGACUCGCCCGA Q708_H04
TCTCTTTACCTTi'CTTGCCG GGGAGGACGAUGCGGUCUCUUUACCUUUCUUGCCGCAGACGACUCGCCCGA 0710_H04
CTTG GGAGTAG GGTGTTCC6 GGGAGGACGAUGCGGCU UGGGAGUAGGG UGU UCCGCAGACGACUCGCCCGA 0711JH04
TACTTATCTCTCTCTTTCCG GGGAGGACGAUGCGGUACUUAUCUCUCUCUUUCCGCAGACGACUCGCCCGA 0712_H04
TTCTTTTCTCGTTTATTCCG GGGAGGACGAUGCGGUUCU UUUCUCGUUUAUUCCGCAGACGACUCGCCCGA 0713_H04
TTTT TGTTACGTTTTTTTGCG GGGAGGACGAUGCGGUUUU LiGU UACGUU UU UUUGCGCAGACGACUCGCCCGA 0714_H04
ACTTTCACTGCTTTTTGCCG GGGAGGACGAUGCGGACUUUCACUGCUUU U UGCCGCAGACGACUCGCCCGA 0716_H04
ATCTTTGTATTTCTTT A CCG GGGAGGAeGAUGCGGAUCUUUGUAUUUC'JUUACCGCAGACGACUCGCCCGA 0717 H04
CTT ITCATTTTTCTTTCCG GGGAGGACGAUGCGGCUUCU UCAUUUUUCUUUCCGCAGACGACUCGCCCGA 0718_H04
TTTCTTTCCTTATTTTCCG GGGAGGACGAUGCGGUU UCUUUCCUUAUU UUCCGCAGACGACUCGCCCGA 0719 H04
S in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
ATTTCTTTCCACTCTTTCCG GGGAGGACGAUGCGGAUUUCUUUCCACUCUUUCCGCAGACGACUCGCCCGA 0720_ .H04
ATTTTATCCTTCTTCTGCCG GGGAGGACGAUGCGGAUUUUAUCCUUCUUCUGCCGCAGACGACUCGCCCGA 0721_ .H04
TTTTTC ACCTTTTG CTTCCG GGGAGGACGAUGCGGU UUUUCACCUUUUGCUUCCGCAGACGACUCGCCCGA 0722_ _H04
CTTACTTTTCGCTTCTTCCG GGGAGGACGAUGCGGCUUACUU UUCGCUUCUUCCGCAGACGACUCGCCCGA 0723. .H04
TTCATTCTTTCTTTTCTGCC GGGAGGACGAUGCGGUUCAUUCUUUCUUUUCUGCCCAGACGACUCGCCCGA 0724. .H04
TTCACTACTTATTTCTTCCG GGGAGGACGAUGCGGUUCACUACUUAUUUCU UCCGCAGACGACUCGCCCGA 0725_ _H04
TTTCACTATTTCTTTCCCGC GGGAGGACGAUGCGGUUUCACUAUUUCUUUCCCGCCAGACGACUCGCCCGA G726. _H04
TATTATTGTTTCTTTCCCCG GGGAGGACGAUGCGGUAUUAUUGUUUCUUUCCCCGCAGACGACUCGCCCGA 0727. _H04
TACTTTTGTTTCTT7TTCCG GGGAGGACGAUGCGGUACU U UUGUUUCUUUUUCCGCAGACGACUCGCCCGA 0728.. „H04
TTTTACTTGTTTTTTGTCCG GGGAGGACGAUGCGGUUUUACUUGUUUU UUGUCCGCAGACGACUCGCCCGA _H04
ATCTTGCTGCTTTTTTACCG GGGAGGACGAUGCGGAUCUUGCUGCUUUUUUACCGCAGACGACUCGCCCGA Q73l_ _HQ4
ATATTTCTTCTTCTTTACCG GGGAGGACGAUGCGGAUAUUUCUUCUUCUUUACCGCAGACGACUCGCCCGA 0732. _HQ4
TT'CGTG CTTTTCTTTCTCCG GGGAGGACGAUGCGGUUCGUGCUUUUCUUUCUCCGCAGACGACUCGCCCGA 0733. ,H04
TTTGTGTTTTTCTTGTTGCG GGGAGGACGAUGCGGU UUG UGUU UUUCUUG UUCCGCAGACGACUCGCCCGA 0734 ,H04
CCATTACTTTTTCCCATCCG GGGAGGACGAUGCGGCCAUUACUUUUUCCCAUCCGCAGACGACUCGCCCGA 0735. .H04
GTTTTCTCTCCTTTGTTCCG GGGAGGACGAUGCGGGUUU UCUCUCCUUUGUUCCGCAGACGACUCGCCCGA 0736. .H04
TTCGTTTTTACTCTTTTCCG GGGAGGACGAUGCGGUUCG UUUUUACUCUUU UCCGCAGACGACUCGCCCGA 0737. _H04
TTTCTTATTATTCTCTCCG GGGAGGACGAUGCGGUUUCUUAUUAUUCUCUCCGCAGACGACUCGCCCGA 0738. _H04
TTTGTT'CTTTTCTTTTCCCC GGGAGGACGAUGCGGUUUGUUCUUUUCUUUUCCCCCAGACGACUCGCCCGA 0739. _H04
CTTTCTTACACTTTGCTCCG GGGAGGACGAUGCGGCUUUCUUACACU UUGCUCCGCAGACGACUCGCCCGA 0740. -H04
TTCTTTTCTCTCTTCGTCCG GGGAGGACGAUGCGGU UCU UUUCUCUCUUCGUCCGCAGACGACUCGCCCGA 0741. -H04
GACTTTCCTTTCTTTriCCC GGGAGGACGAUGCGGGACUUUCCUUUCUU UUUCCCCAGACGACUCGCCCGA 0742. _HG4
TTTCTCTTACATTT CTTCCG GGGAGGACGAUGCGGUUUCUCUUACAUUUCUUCCGCAGACGACUCGCCCGA 0743. .H04
TTTTATTTG TTGTTTGTTG G GGGAGGACGAUGCGGUUUUAU UUGUUGUUUGUUGGCAGACGACUCGCCCGA 0744. _H04
TCTTTTGTTCACTATCTCCG GGGAGGACGAUGCGGUCUUUUGUUCACUAUCUCCGCAGACGACUCGCCCGA 0746, _H04
CTTTTCTCAATTTCGCTCCG GGGA6GACGAUGCGGCUUUUCUCAAU UUCGCUCCGCAGACGACUCGCCCGA 0747. _H04
ACATCTTTTCTTTCCTACCG GGGAGGACGAUGCGGACAUCUUU UCUUUCCUACCGCAGACGACUCGCCCGA 0748. .H04
TTCTTTTCTTACTCCTTCCG GGGAGGACGAUGCGGUUCUUU'JCUUACUCCUUCCGCAGACGACUCGCCCGA 0749 H04
8 in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTGTTCTTCTTATTCCCCG GGGAGGACGAUGCGGU UUGUUCUUCUUAUUCCCCGCAGACGACUCGCCCGA 0750. .H04
TATTTTGTTTTCTGTTTGCG GGGAGGACGAUGCGGUAUU UUGUU UUCUGUUUGCGCAGACGACUGGCCCGA G751. .H04
ATTTG G TCTCTTTTTCG CCG GGGAGGACGAUGCGGAUUUGGUCUC 'UUUUCGCCGCAGACGACUCGCCCGA 0753_ _H04
TTTGTTTATTCCITTTTCCG GGGAGGACGAUGCGGUUUGUUUAUUCCUUUUUCCGCAGACGACUCGCCCGA 075 . .H04
CTTCTTGTTTTGTCTTTCCG GGGAGGACGAUGCGGCUUCUUGUUUUGUCU UUCCGCAGACGACUCGCCCGA 0755. _H04
TTTTATCTTCTTTTGTGCCG GGGAGGACGAUGCGGUUU UAUCUUCUUUUGUGCCGCAGACGACUCGCCCGA 0756. .H04
TTCCGTTCACTTTTTTTCCG GGGAGGACGAUGCG6UUGCGUUCACUUUU UUUCCGCAGACGACUCGCCCGA 0757. .H04
TTTTTCGTG I Π Π Ί I'GCACCG GGGAGGACGAUGCGGU UUUUCGUGUUU UU UUGCACCGCAGACGACUCGCCCGA 0 58^ _HQ4
CATTATTATTTTTCGCTCCG GGGAGGACGAUGCGGCAUUA'JUAUUUUUCGCUCCGCAGACGACUCGCCCGA 0760. .H04
TTTG TTTCTCT7TG TCTCTG GGGAGGACGAUGCGGU UUGUU UCUCUUUGUCUCUGCAGACGACUCGCCCGA 0761. .H04
ACTTCTTACTTTTTCCTCCG GGGAGGACGAUGCGGACUUCUUACUUUUUCCUCCGCAGACGACUCGCCCGA 0762. .H04
ACTTTCTTCTCrfTTTG CCG GGGAGGACGAUGCGGACUUUCUUCUCUUUUUGCCGCAGACGACUCGCCCGA 0763. _H04
CTTTTTTGTTCTTTCATCCG GGGAGGACGAUGCGGCUUUUU UGUUCUUUCAUCCGCAGACGACUCGCCCGA 0764. ^H0
TTTTTGTCTACTTTTTACCG GGGAGGACGAUGCGGUUUU UGUCUACUUUU UACCGCAGACGACUCGCCCGA 0765. .H04
TTTGTACGCG i I I I I I I GTCCG GGGAGGACGAUGCGGUUUGUACGCGUUUUUUUGUCCGCAGACGACUCGCCCGA 0766. _H04
CTTTTTCGTTHT'TCTTCCG GGGAGGACGAUGCGGCUUU UUCGUUUUUUCUUCCGCAGACGACUCGCCCGA 0767. _H04
TTTTACTTTATCTCTTTGTG GGGAGGACGAUGCGGUUUUACUUUAUCUCUU UGUGCAGACGACUCGCCCGA 0768. _H04
TCCTGTCGTCT6TTCCCC GGGAGGACGAUGCGGUCCUG UCGUCU6U UCCCCCAGACGACUCGCCCGA 0770. .H04
CCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 0771. _H04
TCTTTTCTCTCTTCTCACCG GGGAGGACGAUGCGGUCUUUUCUCUCUUCUCACCGCAGACGACUCGCCCGA 0772. _H04
TTTGGGGGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGGGGUAGGGUGUUCCGCAGACGACUCGCCCGA 077 _H04
GTTTTTGTTATTGTTTTGCC GGGAGGACGAUGCGGGUUUUUGUUAUUGUUUUGCCCAGACGACUCGCCCGA 0775. .H04
CTTTCACi Γ! 1 ί I I CCCTCCG GGGAGGACGAUGCGGCU UUCACU UUUUUUCCCUCCGCAGACGACUCGCCCGA 0776. _H04
GTTCTTTGTCTTTTCTCCCG GGGAGGACGAUGCGGGUUCUUUGUCUUUUCUCCCGCAGACGACUCGCCCGA 0777. .H04
GTTTTGTATTCTTTTTCCCC GGGAGGACGAUGCGGGUUU UGUAUUCUUUUUCCCCCAGACGACUCGCCCGA 077S. .H04
CCTTTTACTTTTTTCTCC6 GGGAGGACGAUGCGGCCUUUUACUUUUUUCUCCGCAGACGACUCGCCCGA 0779. _H04
CTATTTACTTTCCTTCTCCG GGGAGGACGAUGCGGCUAUUUACUU UCCUUCUCCGCAGACGACUCGCCCGA 0781. _H04
rGTTCTGTCTTATTTCCG GGGAGGACGAUGCGGUUUGUUCUGUCUUAUUUCC6CAGACGACUCGCCCGA 0782 H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
TTGTGCGTTTTTTTTTTGCCG GGGAGGACGAUGCGGUUGUGCGUUUUU UUUUUGCCGCAGACGACUCGCCCGA 0783_H04
TCTCTTTAATTCTTCTTCCG GGGAGGACGAUGCGGUCUCUUUAAUUCUUCUUCCGCAGACGACUCGCCCGA 0784JH04
TATTTCTCTTTACTTTTCCC GGGAGGACGAUGCGGUAUUUCUCUUUACUUUUCCCCAGACGACUCGCCCGA 0785_ HQ4
T ATTTTAC G TTTTTTTCG CTG GGGAGGAeGAUGCGGUAUUUUACGUUUU UUUCGCUGCAGACGACUCGCCCGA 0786_H04
TCCTGTCGTCGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCG UCGUUCGUCCCCAGACGACUCGCCCGA 0787_H04
CACCTTTTCTTTCTTCTCCG GGGAGGACGAUGCGGCACCUUUUCUUUCU UCUGCGCAGACGACUCGCGCGA 0788„H04
CTTTCTCTTTGTCTTTTCCG GGGAGGACGAUGCGGCUUUCUCUUUG UCUUUUCCGCAGACGACUCGCCCGA 0789_H04
TCTTTCmTTTTATCTCCG GGGAGGACGAUGCGGUCUUUCU UCUU UUAUCUCCGCAGACGACUCGCCCGA 079G_H04
GTTTTTATTCTTTTTCCCCC GGGAGGACGAUGCGGGUUU UUAUUCUUUUUCCCCCCAGACGACUCGCCCGA 0791_H04
GCTTCTTTTCTCTTTTTCCC GGGAGGACGAUGCGGGCUUCUU UUCUCUUUUUCCCCAGACGACUCGCCCGA 0792_H04
TTTACTTTTTTATTGCTCTG GGGAGGACGAUGCGGUUUACUUUUUUAU UGCUCUGCAGACGACUCGCCCGA 0793_H04
TGTTTTACTATTTTTTCCCG GGGAGGACGAUGCGGUG UUUUACUAUUUUUUCCCGCAGACGACUCGCCCGA 0794_H04
AATTTCCTTCTCTTCTACCG GGGAGGACGAUGCGGAAUUUCCUUCUCUUCUACCGCAGACGACUCGCCCGA 0795_H04
TTTATTTCG CGTTTTCTCCG GGGAGGACGAUGCGGUUUAUUUCGCeUUUUCUCCGCAGACGACUCGCCCGA G796_H04
GTTTCTTCACTTTTCTTCCG GGGAGGACGAUGCGGGU UUCUUCACUUUUCUUCCGCAGACGACUCGCCCGA 0797„H04
CTTTCACTTTTTTTTCTCTCCG GGGAGGACGAUGCGGCUUUCACU UUUUUU UCUCUCCGCAGACGACUCGCCCGA 0798_B04
ATATTATTTTTCTCTCGCCG GGGAGGACGAUGCGGAUAUUAU UUUUCUCUCGCCGCAGACGACUCGCCCGA 0?99_H04
TTTCTTTATTCATTCCTCCG GGGAGGACGAUGCGGU U UCUUUAUUCAUUCCUCCGCAGACGACUCGCCCGA 0800_H04
CCTTTTTCGTTTTCTATCCG GGGAGGACGAUGCeGCCUUU UUCGUUUUCUAUCCGCAGACGACUCGCCCGA 0801_H04
ATTTCACTGTC I I I 1 I I CCCG GQGAGGACGAUGCGGAU UUCACUGUCUUUUU UGCCGCAGACGACUCGCCCGA 0802_H04
ACATTTTGTTACTTTTTACTG GGQAGGACGAUGCGGACAUUUUGUUACUUUUUACUGCAGACGACUCGCCCGA 0803_H04
TTTCTTGTCTATTTTTTTCCG GGGAGGAC6AUGCGGUU UCUUGUCUAUUU UU UUCCGCAGACGACUCGCCCGA 0804_H04
TTGTG CGTTTTTTTTTTTGCCG GGGAGGACGAUGCGGUUGUGCGUUUUUUUUUU UGCCGCAGACGACUCGCCCGA 0806_HQ4
TTTTCCAAGCTTTCTCTCCG GGGAGGACGAUGCGG UU UUCCAAGCUU UCUCUCCGCAGACGACUCGCCCGA 0807_,H04
ATTTCTTTGTTTCTTTGCCC GGGAGGACGAUGCGGAUUUCUUUGUUUCUUUGCCCCAGACGACUCGCCCGA 0809 _H04
TATTTTACGTTTTTTCGCTG GGGAGGACGAUGCGGUAUUUUACGUUUU UUCGCUGCAGACGACUCGCCCGA 0810_H04
TTCTCTTTTTCTTTTCACCG GGGAGGACGAUGCGGUUCUCUUUUUCUUUUGACCGCAGACGACUCGCCCGA 0S11_H04
TTTCAC ACTTTTTTTTG CTG GG6AGGACGAUGCGGUUUCACACUUUU UUUUGCUGCAGACGACUCGCCCGA 0812 H04
tt in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ATTTTGTTATATTGTTGCCG GGGAGGACGAUGCGGAUUUUGUUAUAU UGUUGCCGCAGACGACUCGCCCGA 0814. .H04
CTATTTTCTCTCTTACTCCG GGGAGGACGAUGCGGCUAUUU UCUCUCUUACUCCGCAGACGACUCGCCCGA 0815. _H04
TTCACTACTCTTTT CTCCG GGGAGGACGAUSCGGUUCACUACUCUUUUUCUCCGCAGACGACUCGCCCGA 0816. _H04
CTTTTCTCGATTACTTTCCG GGGAGGACGAUGCGGCUUUUCUCGAUUACUU UCCGCAGACGACUCGCCCGA 0817. .H04
TATTTTCTATGCTTTTTCCG GGGAGGACGAUGCGGUAUUUUCUAUGCUUUUUCCGCAGACGACUCGCCCGA 0818. .H04
TACTTTTATTCTTTTTTCCC GGGAGGACGAUGCGGUACUUUUAU UCUU UUUUCCCCAGACGACUCGCCCGA 0819. _H04
TTTTTCTCTCTATCGCTCCG GGGAGGACGAUGCGGU UUUUCUCUCUAUCGCUCCGCAGACGACUCGCCCGA 0820. .H04
AATTCTTTTCTTCTCGTCCG GGGAGGACGAUGCGGAAUUCUUUUCUUCUCGUCCGCAGACGACUCGCCCGA 0821. .H04
ATTTCCGTTCATTTTTGCCG GGGAGGACGAUGCGGAUUUCCGUUCAUUUUUGCCGCAGACGACUCGCCCGA 0822. .H04
TTTGTCATTTTTCTTGTCCG GGGAGGACGAUGCGGUUUGUCAUUUUUCUUGUCCGCAGACGACUCGCCCGA 0823. .H04
TCTTCAACTTTTCTTTTCCG GGGAGGACGAUGCGGUCUUCAACUUUUCUUUUCCGCAGACGACUCGCCCGA 0S24. .H04
ACCACTATTTTCTTTTTCCG GGGAGGACGAUGCGGACCACUAUU UUCUUUUUCCGCAGACGACUCGCCCGA 0825. .H04
TTCTTGTTTAC i ! I ! ! CCC GGGAGGACGAUGCGGUUCUUGUUUACUU UUUUCCCCAGAGGACUCGCCCGA 0826. .H04
o AGTTTTCTTTTTG CTTTTCTG GGGAGGACGAUGCGGAG UUUUCUUUUUGCUUUUCUGCAGACGACUCGCCCGA 0827. _H04
AATTATGTTTTTTTTGTGACCG GGGAGGACGAUGCGGAAUUAUGUUUUUUUUGU6ACCGCAGACGACUCGCCCGA 0828. _H04
TGTCTTTGCTTTTTGTTCCG GGGAGGACGAUGCGGUGUCUUUGCUUUUUG UUCCGCAGACGACUCGCCCGA 0830. _H04
TTCTTG CTCTTTTTCCTCCG GGGAGGACGAUGCGGUUCUUGCUCUUUUUCCUCCGCAGACGACUCGCCCGA 0831. _H04
TTCTACTTGTTTTTCCCCCG GGGAGGACGAUGCGGUUCUAC'JUGUUUUUCCCCCGCAGACGACUCGCCCGA 0832. _H04
T CTTTTTGTATTCTTGTCCG GGGAGGACGAUGCGGUCUUUU UGUAUUCU UCUCCGCAGACGACUCGCCCGA 0833. _H04
TTTTCATACTTTTTCCCCCG GGGAGGACGAUGCGGUUUU A!JACn UlJUUCCCCCGCAGACGACUCGCCCGA 0834. _H04
TTATTTTTGTTTTTTG CGTG GGeAGGACGAUGCGGUUAU UUUUGUUUUUUGCGUGCAGACGACUCGCCCGA 0835. _H04
TTTGGGGTTTTCCCTTGCCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCUUGCCCCAGACGACUCGCCCGA 0837. .H04
ATTTTTTATTTCCTGTGCCG GGGAG6ACGAUGCGGAUUUUUUAU UUCCUGUGCCGCAGACGACUCGCCCGA 0839. .H04
ATCTTCTTATTCTTTCG CCG GGGAGGACGAUGCGGAUCUUCUUAUUCUUUCGCCGCAGACGACUCGCCCGA 0840. ,.H04
TCTTTTTATTrGTGTCTCCG GGGAGGACGAUGCGGUCUUU UUAU UUGLiGUCUCCGCAGACGACUCGCCCGA 0841. _H04
TTTCTATATCCTTCTTTCCG GGGAGGACGAUGCGGUUUCUAUAUCCUUCUUUCCGCAGACGACUCGCCCGA 0842. _H04
GACTTTCTTTTTCTTTTCCC GGGAGGACGAUGCGGGACUUUCUUU UUCUUUUCCCCAGACGACUCGCCCGA 0843. _H04
CTTTTGTCTTTTTCTTTCCG GGGAGGACGAUGCGGCU UUUGUCUUUUUCUUUCCGCAGACGACUCGCCCGA 0844 .H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ATTCCACTTTTTTTTCACCG GGGAGGACGAUGCGGAUUCCACUUUUUUUUCACCCCAQACGACUCGCCCGA QMS. .H04
TTTATTTCTTTTTATCTCCG GGGAGGACGAUGCGGUUUAUUUCUUUUUAUCUCCGCAGACGACUCGCCCGA 0846. _H04
CCTTTTACTTTTTTTTCTCCG GGGAGGACGAUGCGGCCUU U UACU UUUUUUUCUCCGCAGACGACUCGCCCGA 0847. _H04
TTTGTTGTTGT f 1 ! "! "I f I CTG GGGAGGACGAUGCGGU UUGUUGUUGUUUUUUUUCUGCAGACGACUCGCCCGA 0848. _H04
TTTTTTTATTGTTGTTTCTG GGGAGGACGAUGCGGUUUUUU UAUUGUUG UU UCUGCAGACGACUCGCCCGA 0849, .H04
TTTGGGGGTTTCCCGTGCCC GGGAGGACGAUGCGGUUUGGGGGUUUCCCG UGCCCCAGACGACUCGCCCGA 0850. .H04
TATTCTTCTTCTTACCTCCG GGGAGGACGAUGCGGUAUUCUUCUUCUUACCUCCGCAGACGACUCGCCCGA 0851. _H04
ATTCTTCTACTCTT A TTC C G GGGAGGACGAUGCGGAUUCU UCUACUCUUAUUCCGCAGACGACUCGCCCGA 0852. _H04
GTTCTTACTTTATCTTGCCG GGGAGGACGAUGCGGG UUCUUACUUUAUCUUGCCGCAGACGAC UCGCCCGA 0855. _H04
ATTTTCATGTCCCTTTACCG GGGAGGACGAUGCGGAU UUUCAUGUCCCUUUACCGCAGACGACUCGCCCGA 0854. .H04
ATTTTCAACTCCTTTTCCCG GGGAGGACGAUGCGGAU UUUCAACUCCUUU UCCCGCAGACGACUCGCCCGA 0855. .H04
CTATTATCCTTTTCTTTC CG GGGAGGACGAUGCGGCUAUUAUCCUUUUCUUUCCGCAGACGACUCGCCCGA 0857. _H04
CTTTC7TTTCACTTCTTCCG GGGAGGACGAUGCGGCU UUCUUUUCACUUCUUCCGCAGACGACUCGCCCGA 0859. _H04
GTTTCTTTCT ACCTTTTCCG GGGAGGACGAUGCGGGUUUCUUUCUACCUUUUCCGCAGACGACUCGCCCGA 0860. _H04
CTTCTTGCTTTTTTTCCCCCG GGGAGGACGAUGCGGCU UCUUGCUU UUUUUCCCCCGCAGACGACUCGCCCGA 0861. H04
TATTTTTCTTACTTTCCCCG GGGAGGACGAUGCGGUAUUUUUCUUACUUUCCCCGCAGACGACUCGCCCGA 0863. _H04
TTTCTATTTTCTTTTTTCCC GGGAGGACGAUGCG.GUUUCUAUUUUCUUUUUUCCCCAGACGACUCGCCCGA 0864. _H04
TCCAGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCAGUCG UCUGUUCGUCCCCAGACGACUCGCCCGA 0.865. _H04
TCCTGTCGTTGTTCGTCCC GGGAGGACGAUGCGGUCCUG UCGUUGUUCGUCCCCAGACGACUCGCCCGA 0866. _H04
TAATTCTTCCTTTTTCTCCG GGGAGGACGAUGCGGUAAUUCUUCCUUUUUCUCCGCAGACGACUCGCCCGA 0867. .H04
CCTTTATATTACCTTCTCCG GGGAGGACGAUGCGGCCUUUAUAUUACCUUCUCCGCAGACGACUCGCCCGA 0868. .H04
TCACTTTATATTTCTTTCCG GGGAGGACGAUGCGGUCACUUUAUAUUUCUUUCCGCAGACGACUCGCCCGA 0869. _H04
TTTTT ATT ATCTCTG CTCCG GGGAGGACGAUGCGGUUUUUAUUAUCUCUGCUCCGCAGACGACUCGCCCGA 0870. _H04
TATTTATCTTTCTTTCCCCG GGGAGGACGAUGCGGUAUUUAUCUUUCUU UCCCCGCAGACGACUCGCCCGA 0871. .H04
TTCTTTTCACACTTTATCCG GGGAGGACGAUGCGGUUCUU UUCACACUUU.AUCCGCAGACGACUGGCCCGA 0872. .H04
TCTCTTTTGTATTCTTCCCG GGGAGGACGAUGCGGUCUCUUUUCUAUUCUUCCCGCAGACGACUCGCCCGA 0873. .H04
TTTCTT CTATTTTTCTTCC G GGGAGGACGAUGCGGUU UCU UGUAUUUUUCUUCCGCAGACGACUCGCCCGA 0875. _H04
TTTTTACTTTTATTT6CCCG GGGAGGACGAUGCGGUUUUUACUUUUAUUUGCCCGCAGACGACUCGCCCGA 0876 H04
tt in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc mAGCTTTTTTTCCCTCCG GGGAGGACGAUGCGGU UUAGCUUUUUUUCCCUCCGCAGACGACUCGCCCGA 0879. ..H04
TCTTATTCCTTCTCTTTCCG GGGAGGACGAUGCGGUCUUAUUCCUUCUCU UUCCGCAGACGACUCGCCCGA 0880. _H04
ACTTTATTGCCTTTTCACCG GGGAGGACGAUGCGGACUU UAUUGCCUUU UCACCGCAGACGACUCGCCCGA 0881. _H04
TCATTTTATCTTTTTCCCGC GGGAGGACGAUGCGGUCAUUUUAUCUUU UUCCCGCCAGACGACUCGCCCGA 0882. _HG4
TGTTTTCATTTCTCTTTCCG GGGAGGACGAUGCGGUGU UUUCAU U UCUCUUUCCGCAGACGACUCGCCCGA 0883. _H04
TTTCTTTCATTTTTCTACCG GGGAGGACGAU6CGGUUUCUUUCAUU UUUCUACCGCAGACGACUCGCCCGA 0884. _H04
ATCTTTrCAGCTTTTTACCG GGGAGGACGAUGCGGAUCU UUUCAGCUUUUUACCGCAGACGACUCGCCCGA 0885, JH04
ACTTTCTTTCCTTTATTCTG GGGAGGACGAUGCGGACUU UCUUUCCUUUAUUCUGCAGACGACUCGCCCGA 0886_ _H04
TTCTTACGTTTCCCTTTCCG GGGAGGACGAUGCGGUUCU UACGU UUCCCUUUCCGCAGACGACUCGCCCGA 0887. _H04
CTCATTTCCTTTCTTTTCCG G6GAGGACGAUGCGGCUCAU UUCCUUUCUUUUCCGCAGACGACUCGCCCG 0888. _H04
CCATTTCTTTTCTCCTACCG GGGAGGACGAUGCG6CCAUU UCUUUUCUCCUACCGCAGACGACUCGCCCGA 0889. .H04
TTTCTTGATTTTCCCTTGTG GGGAGGACGAUGCGGU UUCUUGAUUUUCCCUUGUGCAGACGACUCGCCCGA 0890. .H04
GTTTACTTTACTTTCCACCG GGGAGGACGAUGCGGGUUUACU UUACUUUCCACCGCAGACGACUCGCCCGA 0891. .H04
ae
ACATTCTC I I 1 1 I ! i'CACCG GGGAGGACGAUGCGGACAUUCUCUUUUUUUCACCGCAGACGACUCGCCCGA 0892. H04
TTACTTTG TTTTTTTCTCCG GGGAGGACGAUGCGGU UACUUUGUUUUUUUCUCCGCAGACGA UCGCCCGA 0893. .H04
TTTGTTATTATTTGTTTCCC GGGAGGACGAUGCGGU UUG UUAUUAUUUGUUUCCCCAGACGACUCGCCCGA 0894. _H04
TTTGGGAGCAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGGAGCAGGGUGU UCCGCAGACGACUCGCCCGA 0895. _H04
rrCTTTATTTTTGCCTCCG GGGAGGACGAUGCGGUUCUUUAUUUUUCCCUCCGCAGACGACUCGCCCGA 0896. .H04
ATTTCTACTTTCTCTTTCCC GGGAGGACGAUGCGGAUUUCUACUUUCUCUUUCCGCAGACGACUCGCCCGA 0898. .H04
ACTTTTTG CTGTTTTTTCCC GGGAGGACGAUGCGGACUUUUUGCUG UUUUU UCCCCAGACGACUCGCCCGA 0899. .H04
TTTTATTCTTTCTTTTTGTG GGGAGGACGAUGCGGUUUUAUUCU UUCU UUUUG UGCAGACGACUCGCCCGA 0900. _H04
TCTCATTCTCTTCT CTCCG GGGAGGACGAUGCGGUCUCAUUCUCUUCUUCUCCGCAGACGACUCGCCCGA 0902. _H04
CCTTTTCTTCTCATTCTCCG GGGAGGACGAUGCGGCCUUU UCU UCUCAUUCUCCGCAGACGACUCGCCCGA 0904. .H04
TTTCAITTTCTCTACTTCCG GGGAGGACGAUGCGGUUUCAUUUUCUCUACUUCCGCA.GACGACUCGCCCGA 0905. _H04
TCTCTTTACTTCCTCTTCCG GGGAGGACGAUGCGGUCUCUUUACUUCCUCUUCCGCAGACGACUCGCCCGA 0906. .H04
ATTTCTCTTTTTTCGTCCCG GGGAGGACGAUGCGGAU UUCUCUUUU UUCGUCCCGCAGACGACUCGCCCGA 0907. _H04
ATTCTTTAATCCTTTTCCCG GGGAGGACGAUGCGGAU UCUUUAAUCCUUUUCCCGCAGACGACUCGCCCGA 0908. -H04
TTATT6 ATTTT6TTTTG CG C GGGAGGACGAUGCGGUUAUUGAULi UUGUUU UGCGCCAGACGACUCGCCCGA 0910 H04
# in H4
NTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CTTCATCTTTTTTCTTACCG GGGAGGACGAUGCGGCU UCAUCUUUU UUCUUACCGCAGACGACUCGCCCGA 0911, H04
ATTTCTCTATCCTTTTTC C C GGGAGGACGAUGCGGAUUUCUCUAUCCUUUUUCCCCAGACGACUCGCCCGA 0912_ ,H04
TTTGCTCTCTATTTTTTCCG GGGAGGACGAUGCGGUUUGCUCUCUAUUU UU UCCGCAGACGACUCGCCCGA 0913. .H04
TTTTCTCTGATTACTTTCCG GGGAGGACGAUGCGGUUUUCUCUGAUUACUUUCCGCAGACGACUCGCCCGA 0914^ .H04
CTTATTATCTCCACTTTCCG GGGAGGACGAUGCGGCUUAUUAUCUCCACUUUCCGCAGACGACUCGCCCGA 0915. _H04
TTTTACTACTTTTTTG CCCG GGGAGGACGAUGCGGUUU UACUACUUUU UUGCCCGCAGACGACUCGCCCGA 0916. _H04
GTTTTTCTTTTCTTTTTCCG GGGAGGACGAUGCGGG UUUUUCUUUUCUUU UUCCGCAGACGACUCGCCCGA 0917. .H04
TTTATTTCTTTTCATGTGTG GGGAGGACGAUGCGGU UUAUUUCU UUUCAUG UGUGCAGACGACUCGCCCGA 0918. .H04
TTCTTCTCTTTTCCCTGCCG GGGAGGACGAUGCGGU UCUUCUCUUUUCCCUGCCGCAGACGACUCGCCCGA 0920. .H04
CTTCTACTCTTATCTTTCCG GGGAGGACGAUGCGGCU UCUACUCUUAUCU UUCCGCAGACGACUCGCCCGA 0922. _H04
TTTCTTTTACGTTTTTTCCC GGGAGGACGAUGCGGUUUCUUUUACGUUUUIJUCCCCAGACGACUCGCCCGA 0923. _H04
A1TTCCTTTTATTTTTACCG GGGAGGACGAUGCGGAUUUCCUUUUAUUUUUACCGCAGACGACUCGCCCGA 0924. _H04
TACTTCTTTTTTTTCTCTGCG GGGAGGACGAUGCGGUACUUCUUUUUUU UCUCUCCGCAGACGACUCGCGCGA 0925, _H04
TCTTTATG GCTTTTTCTCCG GGGAGGACGAUGCGGUCUU UAUGGCUUUUUCUCCGCAGACGACUCGCCCGA 0927. _HQ4
ATTTTTTATTTTTGCTACCG GGGAGGACGAUGCGGAUUUUUUAUUUUUGCUACCGCAGACGACUCGCCCGA 0929, .H04
ATCTTTATTTTTTTCTCTCCG GGGAGGACGAUGCGGAUCUUUAUU UUUUUCUCUCCGCAGACGACUCGCCCGA 0930_ _H04
CTTTTTTTCTTTTG TTCCC G GGGAGGACGAUGCGGCU UUUUULJCUUUUGUUCCCGCAGACGACUCGCCCGA 0931. .H04
TTACTTAT'fTCTT CTCTGCC GGGAGGACGAUGCGGUUACUUAUUUCUUCUCUGCCCAGACGACUCGCCCGA 093 H04
TTATTTTCCCACTCTTTCCG GGGAGGACGAUGCGG U U AU U U UCCCACUCU U UCC6CAG ACGACUCGCCCGA 0935. _H04
TTTGG G AGG AG G GTGTTCCG GGGAGGACGAUGCGGUUUGGGAGGAGGGUG UUCCGCAGACGACUCGCCCGA 0936. JH04
TTTCTTACCATTTCTTTCCG GGGAGGACGAUGCGGUUUCUUACCAUU UCUUUCCGCAGACGACUCGCCCGA 0937. .H04
TTCCATTTTTACTTTCCCCG GGGAGGACGAUGCGGUUCCAUUU UUACUUUCCCCGCAGACGACUCGCCCGA 0938. _H04
'niTCACTTATCTTCTTCCG GGGAGGACGAUGCGGUUUUCACU UAUCUUCUUCCGCAGACGACUCGCCCGA 0939. _HQ4
CTTTTTCTTGCTATTTTCCG GGGAGGACGAUGCGGCUUUUUCUUGCUAUUUUCCGCAGACGACUCGCCCGA 09 0^ H04
ATTTTTTTCCTCTTTTACCG GGGAGGACGAUGCGGAU UUUUUUCCUCUU UUACCGCAGACGACUCGCCCGA 0941. ,H04
TTTTTGTCTTGTTTTTTTCCG GGGAGGACGAUGCGGUUUUUGUCUUGUU UUUUUCCGCAGACGACUCGCCCGA 0942. ,H04
TCCTGTC6TCTGTTCGTTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGUUCCCCAGACGACUCGCCCGA 0943. _H04
TCCAmTTccTTrrrGCCG GGGAGGACGAUGCGG UCCAJUCUUCCUUUU UGCCGCAGACGACUCGCCCGA 09 4^ _H04
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTTCTTG CCCTTTTTTTCCG GGGAGGACGAUGCGGUUUUGU UGCCCUUU U UUUCCGCAGACGACUCGCCCGA 094S. _HQ4
TG CTTTTTTTCTTTTCGCCG GGGAGGACGAUGCGGUGCU UUUUU UCU UUUCGCCGCAGACGACUCGCCCGA 0946. _H04
CTC ATTTCCTATTCT CTCCG GGGAGGACGAUGCGGCUCAUU UCCUAUUCUCUCCGCAGACGACUCGCCCGA 0947. _H04
TTTTTTATTCACCTTCTCCG GGGAGGACGAUGCGGUUU UUUAU UCACCUUCUCCGCAGACGACUCGCCGGA 0948. ,H04
TTTACATTTCTTTTCTTGGC GGGAGGACGAUGCGGUUUACAUUUCU LJUUCUUGCCCAGACGACUCGCCCGA 0950. .H04
CTTCTTTG ACTTTCTTTCCG GGGAGGACGAUGCGGCUUCUUUGACUU UCUU UCCGCAGACGACUCGCCCGA 0951. _H04
TTTTTTTATGGTTTGTTTCCG GGGAGGACGAUGCGGU UU UUUUAUGG UUUGU UUCCGCAGACGACUCGCCCGA 0952. _H04
TTTTTGTTGGTGTTTTTCCG G6GAGGACGAUGCGGU UU UUG UUGG UGUUUUUCC6CAGACGACUCGCCCGA 0953. .K04
CTTTATTTTTG ! I I I I ITGCCC GGGAGGACGAUGCGGCUUUAUUUUUGUUU UUUUGCCCCAGACGACUCGCCCGA 0955. .H04
rrCACTTTATTACTTTTCCC GGGAGGACGAUGCGGUUCACUUUAUUACUUUUCCCCAGACGACUCGCCCGA 0956. _H04
CTTCACTC A ATTTCTCTC CG GGGAGGACGAUGCGGCUUCACUCAAUUUCUCUCCGCAGACGACUCGCCCGA 0959. _H04
TTTTG G G AGTAG G GTGTTCCG GGGAGGACGAUGCGGUUUUGGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 0960. .H04
CTTCTTTTACCTCTCTTCCG GGGAGGACGAUGCGGCUUCUUUUACCUCUCUUCCGCAGACGACUCGCCCGA 0962_ .H04
90
4- CTTTTTCCTTTTCTACTCCG GGGAGGACGAUGCGGCUUUUUCCUUUUCUACUCCGCAGACGACUCGCCCGA 0963_ H04
TTTCTCTTCTTTTGTTCCCG GGGAGGACGAUGCGGU UUCUCUUCUUUUCUUCCCGCAGACGACUCGCCCGA 0964. .H04
ACTTTCATATTTTTTCACCG GGGAGGACGAUGCGGACUUUCAUAUUUUUUCACCGCAGACGACUCGCCCGA 0965. .H04
TTTTTGAACACTTTTTTCTG GGGAGGACGAUGCGGU UUUUGAACACUUUU UUCUGCAGACGACUCGCCCGA 0966. _H04
TTTGGGAGTGGGGTGTTCCG GGGAGGACGAU6CGGU UUGGGAG UGGGGUGU UCCGCAGACGACUCGCCCGA 0967. .H04
ATCTTTCTTTTATCCTGCCG GGCAGGACGAUGCCGAUCUUUCUU UUAUCCUGCCGCAGACGACUCGCCCGA 0969. „H04
CACTTTTCTTCACCTTTCCG GGGAGGACGAUGCGGCACUU UUCUUCACCUUUCCGCAGACGACUCGCCCGA 0970. ,H04
TTTTCGCTGCTTTTTCCCCG GGGAGGACGAUGCGGU UUUCGCUGCUUUUUCCCCGCAGACGACUCGCCCGA 0971. .H04
ATAGTTTTGTTTTTTCGCCG GGGAGGACGAUGCGGAUAGUUUUGUUUUU UCGCCGCAGACGACUCGCCCGA 0972. _H04
TTTTTGTTGATTTG'iTTGCC GGGAGGACGAUGCGGU UUUUGUUGAUUUGUUUGCCCAGACGACUCGCCCGA 0973. .H04
CTTTCTCTTGCTCTTTTCCG GGGAGGACGAUGCGGCUUUCUCUUGCUCUUUUCCGCAGACGACUCGCCCGA 0974. .H04
TCTT7TCTTCTTATTTTCCG GGGAGGACGAUGCGGUCUUUUCU UCUUAUUUUCCGCAGACGACUCGCCCGA 0975. _H04
AATTATGTG I I I i t S I ! GACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUUUUUGACCGCAGACGACUCGCCCGA 0976. _H04
TTTTCTCTTTAATCTTGCCG GGGAGGACGAUGCGGUUUUCUCUUUAAUCUUGCCGCAGACGACUCGCCCGA 0977. „H04
TTTTATTTCACTTTTACCCG GGGAGGACGAUGCGGUUUUAU UUCACUUUUACCCGCAGACGACUCGCCCGA 0979. .H04
# in H4
HTS read variable region RNA sequences tt in H3 selection selection Selected sequenc
TTTTTCGATTTTCTCATCCG GGGAGGACGAUGCGGUUUUUCCAUUUUCUCAUCCGCAGACGACUCGCCCGA 0981. .H04
ATCTTG CTCTTTATTTG CCG GGGAGGACGAUGCGGAUCUUGCUCUUUAUUUGCCGCAGACGACUCGCCCGA 0982_ _H04
TTCTTACTTTTCACCTTCCG GGGAGGACGAUGCGGUUCUUACUUUUCACCUUCCGCAGACGACUCGCCCGA 0983_ _HQ4
TTTTTTCTATCTTCCGTCCG GGGAGGACGAUGCGGUUUUUUCUAUCUUCCGUCCGCAGACGACUCGCCCGA 0984. _H04
CTTTACTGTTTCCTTTTCCG GGGAGGACGAUGCGGCUUUACUGUUUCCUUUUCCGCAGACGACUCGCCCGA 0985. .H04
TCTTTCTTTAACTTTTTCCG GGGAGGACGAUGCGGUCUUUCUUUAACUUUUUCCGCAGACGACUCGCCCGA 0986. .H04
CCTTTTCATTTCTCTTACCG GGGAGGACGAUGCGGCCUUUUCAUUUCUCUUACCGCAGACGACUCGCCCGA Q98 _ _H04
ATCTC TGTTATTTTTCCCG GGGAGGACGAUGCGGAUCUCUUGUUAUUUUUCCCGCAGACGACUCGCCCGA 0988, .H04
TTTG'i "I I I"! rCTCTTTTTCCG GGGAGGACGAUGCGGUUUGUUUUUUCUCUUUUUCCGCAGACGACUCGCCCGA 0989_ .H04
TTCTTTGTTTTTTTCTCCCG GGGAGGACGAUGCGGUUCUUUGUUUUUUUCUCCCGCAGACGACUCGCCCGA 0990. _H04
TTrrcTTCACTTTTTTCCCG GGGAGGACGAUGCGGUUUUCUUCACUUUUUUCCCGCAGACGACUCGCCCGA 0991. H04
TTTTATCCTCGTTTTCTCCG GGGAGGACGAUGCGGUUUUAUCCUCGUUUUCUCCGCAGACGACUCGCCCGA 0992.. .H04
TTTCACTCTTTTTTTCCCCGC GGGAGGACGAUGCGGUUUCACUCUUUUUUUCCCCGCCAGACGACUCGCCCGA 0993. _H04
GTTTTG CTCCTTTTTCGCCG GGGAGGACGAUGCGGGUUUUGCUCCUUUUUCGCCGCAGACGACUCGCCCGA 0994. _H04
CCTTATGTTTTCCCTTTCCG GGGAGGACGAUGCGGCCUUAUGUUUUCCCUUUCCGCAGACGACUCGCCCGA 0995. .H04
ACTTTTCCCTATCTTTTCCG GGGAGGACGAUGCGGACUUUUCCCUAUCUUUUCCGCAGACGACUCGCCCGA 0996. _H04
TATTTTTCACGTTTCCTCCG GGGAGGACGAUGCGGUAUUUUUCACGUUUCCUCCGCAGACGACUCGCCCGA 0997. JH04
CTTTTCC TATTTCTACTCCG GGGAGGACGAUGCGGCUUUUCCUAUUUCUACUCCGCAGACGACUCGCCCGA 0998. _H04
mOTGmnrcTTCCG GGGAGGACGAUGCGGUUUCUUGUUCUUUCUUCCGCAGACGACUCGCCCGA 0999. .H04
GTTCTTATTATTCTC ACCG GGGAGGACGAUGCGGGUUCUUAUUAUUCUCUACCGCAGACGACUCGCCCGA 1000. H04.
ATTTTTGACTTTTCTTACCG GGGAGGACGAUGCGGAUUUUUGACUUUUCUUACCGCAGACGACUCGCCCGA 1001. .H04
TCTTTTTCTCG CCTTATCCG GGGAGGACGAUGCGGUCUUUUUCUCGCCUUAUCCGCAGACGACUCGCCCGA 1002. _H04
ATTTTATTTATTCG CTACCG GGGAGGACGAUGCGGAUUUUAUUUAUUCGCUACCGCAGACGACUCGCCCGA 1003. .H04
AATTTTCTATCCmTACCG GGGAGGACGAUGCGGAAUUUUCUAUCCUUUUACCGCAGACGACUCGCCCGA 1004. .H04
TCTTTTTTTTGTTTCCCCCG GGGAGGACGAUGCGGUCUUUUUUUUGUUUCCCCCGCAGACGACUCGCCCGA 1005. _HG4
CTTCATACTTTTTTCTTCCG GGGAGGACGAUGCGGCUUCAUACUUUUUUCUUCCGCAGACGACUCGCCCGA 1006. _H04
TTTTTTAATGTTTTTTTCCG GGGAGGACGAUGCGGUUUUUUAAU6UUUUUUUCCGCAGACGACUCGCCCGA 1007. .H04
TTGTGCGT! H I Π NGCCG GGGAGGACGAUGCGGUUGUGCGUUUUUUUUUGCCGCAGACGACUCGCCCGA 1009. .H04
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TTGTTGTTTTATTGGTTCCG GGGAGGACGAUGCGGU UGUUGL'UUUAUUGGUUCCGCAGACGACUCGCCCGA 1010, _H04
ATTTGmAATTTTGTTCCC GGGAGGACGAUGCGGAUUUGUUUAAUUUUGUUCCCCAGACGACUCGCCCGA 1011. H04
TTACTTATTTATTTGGTCCG GGGAGGACGAUGCGGUUACUUAUUUAUUUGCUCCGCAGACGACUCGCCCGA 1012_ _H04
TCTGTTACACTTTTTTTCCG GGGAGGACGAUGCGGUCUG UUACACUUUUUUUCCGCAGACGACUCGCCCGA 1013. _H04
CTTCTATTTTTCGCTTACCG GGGAGGACGAUGCGGCUUCUAUUUUUCGCUUACCGCAGACGACUCGCCCGA 1014. HQ4
ATTTCCTTGTTTTTGTTGTG GGGAGGACGAUGCGGAUUUCCUUGUUUUUGUUG UGCAGACGACUCGCCCGA 1015. _MQ4
TTTCTTTCA i ! I i I t ! I CCG GGGAGGACGAUCCGCUUUCUUUCAUUUUUUUUCCGCAGACGACUCGCCCGA 1016. _HQ4
CTTTTCTATCTTTTCTTCCG GGGAGGACGAUGCGGCUUUUCUAUCUUUUCUUCCGCAGACGACUCGCCCGA 1017. ..H04
TATTCTCTTGCTTTGCTCCG GGGAGGACGAUGCGGUAUUCUCUUGCUUUGCUCCGCAGACGACUCGCCCGA 1019. .H04
TCTGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUCUGGGAGUAGGG UGUUCCGCAGACGACUCGCCCGA 1021. _H04
ATTAGGTG TAGGGTGGACTG GGGAGGACGAUGCGGAUUAGGUGUAGGGUGGACUGCAGACGACUCGCCCGA 1Q22. .H04
ATTATCG7TTTCTTTCACCG GGGAGGACGAUGCGGAUUAUCGUUUUCUUUCACCGCAGACGACUCGCCCGA 1023. H04
CTTTTTrrcTCCTTTTGCTG GGGAGGACGAUGCGGCUUU UUUUCUCCUUUUGCUGCAGACGACUCGCCCGA 1024. H04
TCATCTTTGTCTTTTTTCCG GGGAGGACGAUGCGGUCAUCUUUGUCUUUUUUCCGCAGACGACUCGCCCGA 1025. H04
GTT GTTGGTTTTTTCCG GGGAGGACGAUGCGGG UUUUUGUUGGUUU UUUCCGCAGACGACUCGCCCGA 1026. .H04
TTT ATTTCTC ACTTTTTG CG GGGAGGACGAUGCGGU UUAUU UCUCACUUUUUGCGCAGACGACUCGCCCGA 1027 H04
ATTTTGCT'1 1 1 ! IACTACC6 GGGAGGACGAUGCGGAUUUUGCUUUUUUACUACCGCAGACGACUCGCCCGA 1029. _H04
ATTTTACTGTTCTCTTTCCC GGGAGGACGAUGCGGAUUU UACUGUUCUCUUUCCCCAGACGACUCGCCCGA 1032. _H04
ATTTTCCATGGCTTTTACCG GGGAGGACGAUGCGGAUUU UCCAUGGCUU UUACCGCAGACGACUCGCCCGA 1033 H04
TTTTTACCCTTTTTTTTGTCCG GGGAGGACGAUGCGGUUUUUACCCUUUUUUU UGUCCGCAGACGACUCGCCCGA 1.034, „H04
ATCATCACTTTCTTTTACCG GGGAGGACGAUGCGGAUCAUCACUUUCUUUUACCGCAGACGACUCGCCCGA 1035. .H04
TTGTTTCTTTATTTTTCC CG GGGAGGACGAUGCGGUUGUUUCUUUAUUUUUCCCGCAGACGACUCGCCCGA 1036_ _H04
TCTTTTTAAC GTTTCCTCCG GGGAGGACGAUGCGGUCUU UUUAACGUUUCCUCCGCAGACGACUCGCCCGA 1037. _H04
TTTTCACTTTTTTTCGGCTG GGGAGGACGAUGCGGUU UUCACUUUUUUUCGGCUGCAGACGACUCGCCCGA 1038. H04
rcT rGTCATCTCTCCG GGGAGGACGAUGCGGUUCU UUUUGUCAUCUCUCCGCAGACGACUCGCCCGA 1039, _H04
TTTCTCAACTATCTTTTCCG GG'GAGGACGAUGCGGUUUCUCAACUAUCUU UUCCGCAGACGACUCGCCCGA 1040. _H04
TTCTTTGTTTTATTATTCCG GGGAGGACGAUGCGGUUCUUUG UUUUAU UAUUCCGCAGACGACUCGCCCGA 1041. . H04
TGTTTTATTTGTTTTGCGTG GGGAGGACGAUGCGGUGUU UUAUUUGUUUUGCGUGCAGACGACUCGCCCGA 1042 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc TTTGGGAGTAGGGAGTTCCG GCGAGGACGAUGCGCU UUGGGAGUAGGGAGUUCCGCAGACGACUCGCCCGA 1044_H04
TTTG GGAGAAG GGTGTTCCG GGGAGGACGAUGCGGU UUGGGAGAAGGGUGUUCCGCAGACGACUCGCCCGA 1045 H04
TG CTTTATTTTTCC TT CCCG GGGAGGACGAUGCGGUGCUUUAUUUUUCCUUCCCGCAGACGACUCGCCCGA 1046_H04
TTTGTTTTATTTTTGTCCC GGGAGGACGAUGCGGUUUG UU UUAUUUUUGUCCCCAGACGACUCGCCCGA 1047_H04
CTTTTCACTTTTTTCTTCCG GGGAGGACGAUGCGGCUUUUCACUU UUUUCUUCCGCAGACGACUCGCCCGA 1048.. H04
TTTTATCTTTTGTTTTTG CG GGGAGGACGAUGCGGUUUUAUCUUUUGUUUUUGCGCAGACGACUCGCCCGA 1049_H04
CGATTCCTCTGGCTCGTCCC GGGAGGACGAUGCGGCGAUUCCUCUGGCUCG UCCCCAGACGACUCGCCCGA 1050_H04
TATCTTCTCTTTCTCATCCG GGGAGGACGAUGCGGUAUCU UCUCU UUCUCAUCCGCAGACGACUCGCCCGA 1052_H04
TTTCCTTTTAACTTGCTCCG GGGAGGACGAUGCGGUUUCCUUUUAACUUGCUCCGCAGACGACUCGCCCGA 1053_H04
ATTTT G GTTATTTTTG G CCG GGGAGGACGAUGCGGAU UUUGGUUAUUUUUGGCCGCAGACGACUCGCCCGA 1055_H04
ATTCTC ! I i I ! I !TTCCCACCG GGGAGGACGAUGCGGAU UCUCUUUUUUUUUCC ACCGCAGACGACUCGCCCGA 1056JH04
GITTTGTCTTTTTCCTTCCG GGGAGGACGAUGCGGGUUUUGUCUULiUUCCUUCCGCAGACGACUCGCCCGA 1057JH04
TTC ACTTTTTTCTCTTG CTG GGGAGGACGAUGCGGUUCACUUUUUUCUCUUGCUGCAGACGACUCGCCCGA 1058_H04
TATTGCTCTTTTATTTTCCC GGGAGGACGAUGCGGUAUUGCUCUUUUAUUU UCCCCAGACGACUCGCCCGA 1059_H04
TTCCACTM ΊΊΊ 1 1 j'CTCCG GGGAGGACGAUGCGGUUCCACUUUUUUUUUCUCCGCAGACGACUCGCCCGA 1060JH04
TTTTTG TA A G TTTTTTCCC GGGAGGACGAUGCGGUUUU UG UAAUGUUUU UUCCCCAGACGACUCGCCCGA 1Q61_H04
TTTATTTTCACTTTTTCCCG GGGAGGACGAUGCGG UULiAUUUUCACUUUUUCCCGCAGACGACUCGCCCGA 1G62JH04
ATATT ATTTTGTTTTTTG C C GGGAGGACGAUGCGGAUAUUAUUU UGUUUU 'JUGCCCAGACGACUCGCCCGA 1063_H04
TTTTCTTACGCGTTTTTCCG GGGAG6ACGAUGCGGUUUUCUUACGCGUUUUUCCGCAGACGACUCGCCCGA 1064_.H04
TCACTTTCTATTCCCTTCCG GGGAGGACGAUGCGGUCACUUUCUAUUCCCUUCCGCAGACGACUCGCCCGA 1Q6S_H04
GATAGAGGAGGTGGTGCCTG GGGAGGACGAUGCGGGAUAGAGGAGGUGGUGCCUGCAGACGACUCGCCCGA 1066_H04
TTTCTCAGTCTTCTTTTCC6 GGGAGGACGAUGCGGU U UCUCAGUCUUCUUUUCCGCAGACGACUCGCCCGA 1067_H04
C ATTTTC CTAGTTTTCTCCG GGGAGGACGAUGCGGCAU UUUCCUAGUUUUCUCCGCAGACGACUCGCCCGA 1068_H04
TCCTGTCGTCTGTTCCGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUGUUCCGUCCCCAGACGACUCGCCCGA 1069_H04
AATTATGTGTTTTTTGT6ACCT GGGAGGACGAUGCGGAAUUAUGUGUUUUUUGUGACCUCAGACGACUCGCCCGA 1070_H04
TCATTATTTC7TCTCCTCCG GGGAGGACGAUGCGGUCAUUAUUUCUUCUCCUCCGCAGACGACUCGCCCGA 1073_H04
CCTTTATTATACTCTCTCCG GGGAGGACGAUGCGGCCUUUAUUAUACUCUCUCCGCAGACGACUCGCCCGA 1074_H04
ACTACTCG GCATTGGTGCCC GGGAGGACGAUGCGGACUACUCGGCAUUGGUGCCCCAGACGACUCGCCCGA 1075 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTCGCTACAC I 1 1 ! f TCC6 GGGAGGACGAUGCGGU UUCGCUACACUU U UUUCCGCAGACGACUCGCCCGA 1076_ H04
TCTTTTTTTTG TG TTTCTCCG GGGAGGACGAUGCGGUCUU UUUUUUGUGUUUCUCCGCAGACGACUCGCCCGA 1077. .H04
CTATCCTTGT ΓΠΊ ' I CTTCCG GGGAGGACGAUGCGGCUAUCCUUGUUUUUUCUUCCGCAGACGACUCGCCCGA 1078.. .BQ4
TTTACT mCTATCTTTCCG GGGAGGACGAUGCGGU UUACU UUUCUAUCUU UCCGCAGACGACUCGCCCGA 1079. _H04
GATTT1 rCGCTGTTTTACCC GGGAGGACGAUGCGGGAUU UUUCGCUGUUUUACCCCAGACGACUCGCCCGA 1080. .H04
T1TGTTTTTTTGTTTCTG GGGAGGACGAUGCGGUUUGUUUUUUUGUUUCUGCAGACGACUCGCCCGA 1081. H04
TTTCAACTTTTTCATTTCCC GGGAGGACGAUGCGGUUUCAACUUUUUCAUU UCCCCAGACGACUCGCCCGA 1082. H04
TTTCATTTATTCTTTCTGTG GGGAGGACGAUGCGGUUUCAU UUAUUCU UUCUGUGCAGACGACUCGCCCGA 1083. _H04
CAT AG G AGTAG 6GTG GTG G C GGGAGGACGAUGCGGCAUAGGAGUAGGGUGGUGGCCAGACGACUCGCCCGA 1084. .HQ4
CTTTTCTATTTCTC CTTCCG GGGAGGACGAUGCGGCUUUUCUAUUUCUCCUUCCGCAGACGACUCGCCCGA 1086. .H04
GTTTCTTCTTCTTATTTCCG GGGAGGACGAUGCGGGUUUCUUCUUCUUAUUUCCGCAGACGACUCGCCCGA 1087. .H04
CTTTACTTTTTATCTCACCG GGGAGGACGAUGCGGCUUUACUUUUUAUCUCACCGCAGACGACUCGCCCGA 1088. .H04
ATTCTTTCTTTTCTGTCCCG GGGAGGACGAUGCGGAUUCUUUCUUUUCUGUCCCGCAGACGACUCGCCCGA 1089. .H04
TTTCTTTGTTTGTTTTCGT6 GGGAGGACGAUGCGGU UUCUUUGUUUG UUUUCGUGCAGACGACUCGCCCGA 1090. _H04
GTTTTTCATTATTCTTTCCC GGGAGGACGAUGCGGGUUU UUCAUUAUUCU UUCCCCAGACGACUCGCCCGA 1091. .H04
TCCTGTCTCTGTTCGTC C C GGGAGGACGAUGCGGUCCUGUCUCUGUUCGUCCCCAGACGACUCGCCCGA 1093. _H04
ATTACTCTTCTCTCTTG CCG GGGAGGACGAUGCGGAUUACUCUUCUCUCUUGCCGCAGACGACUCGCCCGA 1094 H04
CGCTTTTTGTTTTTCCTCGC GGGAGGACGAUGCGGCGCUUUUUGUUUUUCCUCGCCAGACGACUCGCCCGA 1095. _H04
GTCTTGTCTATTTTTCCCCG GGGAGGACGAUGCGGGUCU UGUCUAUUU UUCCCCGCAGACGACUCGCCCGA 1097. _H04
ACTTTCTCTTCTTTTATCCC GGGAGGACGAUGCGGACUUUCUCUUCUUUUAUCCCCAGACGACUCGCCCGA 1098. .HQ4
TTTCCTTT'i'T'i'TTCCCATCCG GGGAGGACGAUGCGGUUUCCUU UUUUUUCCCAUCCGCAGACGACUCGCCCGA 1099. .H04
TCTTTTATTCTTCTTTACCG GGGAGGACGAUGCGGUCUUUUAUUCUUCUUUACCGCAGACGACUCGCCCGA 1100. _H04
T CTGTCGTGTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUGUGUUCGUCCCCAGACGACUCGCCCGA 1101. _H04
ATTCTTGCTTTTiTGCCG GGGAGGACGAUGCGGAU UCU UGCUUUUUUGCCGCAGACGACUCGCCCGA 1103. _H04
ATCTTTTTACACTCTCACCG GGGAGGACGAUGCGGAUCUUUUUACACUCUCACCGCAGACGACUCGCCCGA 1104. .H04
CTTTTTACACTTTTATCCCG GGGAGGACGAUGCGGCU UUUUACACUUUUAUCCCGCAGACGACUCGCCCGA 1105. .H04
ATTTTATTTCCCTTTCCCCG GGGAGGACGAUGCGGAUUUUAUUUCCCUUUCCCCGCAGACGACUCGCCCGA 1106. „H04
TTTTCCATTTTTTCACTCCG GGGAGGACGAUGCGGUUUUCCAUUUUUUCACUCCGCAGACGACUCGCCCGA 1107. _H04
in H4
HTS read variab!e region RNA sequences lection selection Selected sequenc
CTTTT ATTATTTG TTT A CC G GGGAGGACGAUGCGGCUUU UAUUAUUUGUU UACCGCAGACGACUCGCCCGA 1109_H04
TCTTATTTCTTTCTTCCCCG GGGAGGACGAUGCGGUCUUAUUUCUUUCUUCCCCGCAGACGACUCGCCCGA 1110_H04
TTTCTTTGTTTCTTTTCCTG GGGAGGACGAUGCGGUUUCUUUGUUUCUUU UCCUGCAGACGACUCGCCCGA mi_M04
TTTG GGGTTTTCCCGCG CCC GGGAGGACGAUGCGGU UUGGGGU UUUCCCGCGCCCCAGACGACUCGCCCGA 1U2_H04
6πGGGGτTTTCCCGTGCCC GGGAGGACGAUGCGGG UUGGGGUUUUCCCGUGCCCCAGACGACUCGCCCGA 1113_H04
CAATTGGTGGAGGTGGGCTG GGGAGGACGAUGCGGCAAUUGGUGGAGGUGGGCUGCAGACGACUCGCCCGA 1114_H04
ATTTCTCGATCTTTTCTCCG GGGAGGACGAUGCGGAUUUCUCGAUCUUUUCUCCGCAGACGACUCGCCCGA 1U5_H04
TTTACTCTCTTACTTCTCCG GGGAGGACGAUGCGGUUUACUCUCU UACUUCUCCGCAGACGACUCGCCCGA 1117_H04
nTTTCGTGTTTTTTGCACCG GGGAGGACGAUGCGGUUUU UCGUGUUUUUUGCACCGCAGACGACUCGCCCGA 1118_H04
TTTGTTG CCCCTTTTTTCCG GGGAGGACGAUGCGGUUUGUUGCCCCUU U UUUCCGCAGACGACUCGCCCGA 1119_ HG4
TTTTAACATTACCTTTTCCC GGGAGGACGAUGCGGUUUUAACAUUACCU UUUCCCCAGACGACUCGCCCGA 1120_H04
ATTCCTACTTTTTTTCCCCG GGGAGGACGAUGCGGAU UCGUACUUUUUU UCCCCGCAGACGACUCGCCCGA 1121_H04
CCTTTTCATTTTCTATTCCG GGGAGGACGAUGCGGCCUUUUCAUUUUCUAUUCCGCAGACGACUCGCCCGA 1122_H04
ITTrCACTTTTTCTTTTGCC GGGAGGACGAUGCGGUUUUCACUUUUUCUUUUGCCCAGACGACUCGCCCGA 1123_H04
TTTTTTCATTATCTTG TCCC GGGAGGACGAUGCGGUUUUUUCAU UAUCUUGUCCCCAGACGACUCGCCCGA 1124_H04
CAAAGGGAGGTGTAGGGTGC GGGAGGACGAUGCGGCAAAGGGAGGUGUAGGGUGCCAGACGACUCGCCCGA 1125_H04
G TTCTTAG TCTTTCTTTCCG GGGAGGACGAUGCGGGUUCUUAGUCUUUCUUUCCGCAGACGACUCGCCCGA 1127JH04
TTTATTTCTTTCCAC G TTCCT GGGAGGACGAUGCGGUUUAUUUCUUUCCACGUUCCUCAGACGACUCGCCCGA 1128„H04
TTTTACAAAATTTCGCTCCG GGGAGGACGAUGCGGUUUUACA.AAAUUUCGCUCCGCAGACGACUCGCCCGA 1129_H04
TCTTTACrrTTTTTCACCCG GGGAGGACGAUGCGGUCUUUACUUUUUUUCACCCGCAGACGACUCGCCCGA 1130_H04
TT ATTTTTTG CCTG CTTC CG GGGAGGACGAUGCGGUUAUUUUUUGCCUGCUUCCGCAGACGACUCGCCCGA 1131JH04
TTTTTTATACACCTTTTCCG GGGAGGACGAUGCGGUUUU UUAUACACCU UUUCCGCAGACGACUCGCCCGA 1132_HQ4
TTTITATTTCGTGTTTTTGCG GGGAGGACGAUGCGGUUUUUAUUUCGUGUU UUUGCGCAGACGACUCGCCCGA 1133_H04
TTCTTTATTTCTATCTTCCG GGGAGGACGAUGCGGU UCUUUAUUUCUAUCUUCCGCAGACGACUCGCCCGA 113 H04
TCCTTTCGTTCTTCCCTCCC GGGAGGACGAUGCGGUCCUU UCGUUCUUCCCUCCCCAGACGACUCGCCCGA 1135„H04
TTCCATTCG CTTTTTCTCCG GGGAGGACGAUGCGGUUCCAUUCGCUUUUUCUCCGCAGACGACUCGCCCGA 1136_H04
CATTACTTCTTCTTCTCCG GGGAGGACGAUGCGGCAUUACUUCUUCUUCUCCGCAGACGACUCGCCCGA 1137_H04
TTTTCTTGTTG TTTTTG CCC GGGAGGACGAUGCGGUUUUCUUGUUGUUUUUGCCCCAGACGACUCGCCCGA 1138 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CTTTATTTCACTCTTTACCG GGGAGGACGAUGCGGCUUUAUUUCACUCUUUACCGCAGACGACUCGCCCGA 1139. _HQ4
CTTCTTTGTTCGTTTTTCCG GGGAGGACGAUGCGGCUUCUUUGUUCGUUUUUCCGCAGACGACUCGCCCGA 1140. _H04
TTTTCGCTTTTCTTTATCCG GGGAGGACGAUGCGGUUU UCGCUUU UCU UUAUCCGCAGACGACUCGCCCGA 1141. _H04
TCTCTTATAACTTCTTTCCG GGGAGGACGAUGCGGUCUCUUAUAACUUCUUUCCGCAGACGACUCGCCCGA 1142. .H04
TTTTATCTCTG TTTTTTGCC GGGAGGACGAUGCGGU UUUAUCUCUGUUUU UUGCCCAGACGACUCGCCCGA 1143_ .H04
TTTTTC C A ΓΤΤ C77 TAG C CG GGGAGGACGAUGCGGU UUUUCCAUUUCU UUACCCGCAGACGACUCGCCCGA 1144, H04
TTCATTTGTTCT TTTTCCCG GGGAGGACGAUGCGGUUCAUUUGUUCUUUUUCGCGCAGACGACUCGCCCGA 1145. _H04
TTTTTCGCCATI TTTCCCCG GGGAGGACGAUGCGGU UUU UCGCCAU UUU UCCCCGCAGACGACUCGCCCGA 1146. .H04
TTCCAC I ! I ! I I ! I ITCTCCG GGGAGGACGAUGCGGUUCCACUUUUU UUUUUCUCCGCAGACGACUCGCCCGA 1147. _H04
rCTTTTTTCTACCG GGGAGGACGAUGCGGCCUUUUUCUUUUUUCUACCGCAGACGACUCGCCCGA 1148_ „H04
rCTTCTTTCTCCG GGGAGGACGAUGCGGUCUUUUUUCUUCUUUCUCCGCAGACGACUCGCCCGA 1149. .H04 TCTTTTTCTACrrTTACCCG GGGAGGACGAUGCGGUCUUUUUCUACUUUUACCCGCAGACGACUCGCCCGA 1150_ .H04
CTTTATTATTTTCCTTCCCG GGGAGGACGAUGCGGCUUUAUUAUUUUCCUUCCCGCAGACGACUCGCCCGA 1151. .H04
o GTrCCATTTCTCTTTTGCCG GGGAGGACGAUb GGG UUCCAUUUCUCUU UUGCCGCAGACGACUCGCCCGA 1152. .H04
TTTTTCTTCTTTTAGTrCCG GGGAGGACGAUGCGGUUUUUCUUCUUU UAGUUCCGCAGACGACUCGCCCGA 1153. _H0
TGTTTTTTTCCCTTTCCCCG GGGAGGACGAUGCGGUGUUUUUUUCCCUUUCCCCGCAGACGACUCGCCCGA 1154. .H04
GTATCTCTTTTCTTTTCTCCG GGGAGGACGAUGCGGGUAUCUCUUUUCUUUUCUCCGCAGACGACUCGCCCGA 1155. ,H04
TCTTTTCATTGCTCTTTCCG GGGAGGACGAUGCGGUCUU UUCAU UGCUCUUUCCGCAGACGACUCGCCCGA 1156. .H04
TTTTTTTCTCCGGTTTTCCG GGGAGGACGAUGCGGU UUUUU UCUCCGGUUU UCCGCAGACGACUCGCCCGA 1157. .H04
ACCTTTCTAATCT7TCTCCG GGGAGGACGAUGCGGACCUUUCUAAUCUUUCUCCGCAGACGACUCGCCCGA 1158. .H04
ATCCTTTTTGTTTTTCGCCG GGGAGGACGAUGCGGAUCCUUUUUGUUU UUCGCCGCAGACGACUCGCCCGA 1159. _H04
TTTTTTCTATCTCTTCTCCG GGGAGGACGAUGCGGUUUU UUCUAUCUCUUCUCCGCAGACGACUCGCCCGA. 1160. _H04
TTTTCATGACTTTTTGCCCG GGGAGGACGAUGCGGUUUUCAUGACUUUUUGCCCGCAGACGACUCGCCCGA 1161. H04
ATTCTTGCTTTTTTTTTGCCG GGGAGGACGAUGCGGAUUCUUGCUUU UUUUUUGCCGCAGACGACUCGCCCGA 1162. HOA
TTTACTGTTTCCTTTTTCGC GGGAGGACGAUGCGGUUUACUGUUUCCUU UUUCGCCAGACGACUCGCCCGA 1163. .H04
CTTTTTATTCTTTTTCACCG GGGAGGACGAUGCGGCUUU UUAUUCU UUUUCACCGCAGACGACUCGCCCGA 1 164. .H04
ACTTTTG TT ACTTTTT ACCC GGGAGGACGAUGCGGACUUUUGUUACUUUUUACCCCAGACGACUCGCCCGA 1165. .H04
TTACCTTTCCTTTTACTCCG GGGAGGACGAUGCGGUUACCUUUCCUUUUACUCCGCAGACGACUCGCCCGA 1166. .H04
tf in H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc TTTATTTTCCTGTGCTTCCG GGGAGGACGAUGCGGUUUAUUUUCCUGUGCUUCCGCAGACGACUCGCCCGA 1167. _H04
CITATTTCATTCCTCTTCCG G.GGAGGACGAUGCGGCUUAUUUCAUUCCUCUUCCGCAGACGACUCG'CCCGA 1168. _H04
AATTTC TCG TTTTTTTG CTG GGGAGGACGAUGCGGAAUUUCUCGUUUU UUUGCUGCAGACGACUCGCCCGA 1169. H04
TCATTACTGTTTTTTTATCCG GGGAGGACGAUGCGGUCAU UACUGUUUUUUUAUCCGCAGACGACUCGCCCGA 1170. _H04
CCTATTTCTTTCTTCTTCCG GGGAGGACGAUGCGGCCUAU UUCUUUCUUCUUCCGCAGACGACUCGCCCGA 1171. H04
TTTTTACT I TGGTCCC GGGAGGACGAUGCGGUUUUUACUCUUUUUGGUCCCCAGACGACUCGCCCGA 1172. .H04
ACCTTTTGTC7 TTTACCG GGGAGGACGAUGCGGACCLJUUUGUCUUUUUUACCGCAGACGACUCGCCCGA 1174. .H04
TATTTTCTACCTTTTTTCCG GGGAGGACGAUGCGGUAUUUUCUACCUUUUUUCCGCAGACGACUCGCCCGA 1175, _H04
TATTTCTTTTATTCCTTGTG GGGAGGACGAUGCGGUAUUUCUUUUAUUCCUUG UGCAGACGACUCGCCCGA 1176. .H04
ACTFTTTTTCTCTCCTTTCCG GGGAGGACGAUGCGGACUUUU UUUCUCUCCUUUCCGCAGACGACUCGCCCGA 1177. .H04
ATTTTTCATTTGCTCTACCG GGGAGGACGAUGCGGAUUUUUCAUUUGCUCUACCGCAGACGACUCGCCCGA 1178. _H04
TTATTTACCTTTCCTTTCCG GGGAGGACGAUGCGGUUAUUUACCUUUCCUUUCCGCAGACGACUCGCCCGA 1180 H04
TTCTTITTG TTTTTCCCCGG GGGAGGACGAUGCGGUUCUUUUUGUUUUUCCCCGGCAGACGACUCGCCCGA 1181. H04
TTATTGCTTTTTTTG GTCCG GGGAGGACGAUGCGGUUAU UGCUUUUUUUGGUCCG AGACGACUCGCCCGA 1182. .H04
TTATTT A A CTCCTTTCTC CG GGGAGGACGAUGCGGUUAUUUAACUCCUUUCUCCGCAGACGACUCGCCCGA 1183, _H04
CATTCTTCTGTTTTCCTTCGC GGGAGGACGAUGCGGCAUUCUUCUG UUUUCCU UCGCCAGACGACUCGCCCGA 1184 _H04
TTTC ACTTTTAJTCCTG CCG GGGAGGACGAUGCGGUUUCACU UUUAU UCCUGCCGCAGACGACUCGCCCGA. 1185. .H04
CTTTCACTTTTTTCTCTCCG GGGAGGACGAUGCGGCUUUCACU UU UUUCUCUCCGCAGACGACUCGCCCGA 1186. .H04
TTTTTAATGTTCTTCGTCCG GGGAGGACGAUGCGGU UUU UAAUGUUC'dUCG UCCGCAGACGACUCGCCCGA 1187. .H04
TGTTTTTTCTATTGTTCCCG GGGAGGACGAUGCGGUGUUUUUUCUAUUGUUCCCGCAGACGACUCGCCCGA 1188. _H04
TCTTTAATTCGCTTTTCCCG GGGAGGACGAUGCGGUCUU UAAUUCGCUUUUCCCGCAGACGACUCGCCCGA 1189. _H04
GTTTATTATCTTTCCTACCG GGGAGGACGAUGCGGGU UUAUUAUCUUUCCUACCGCAGACGACUGGCCCGA 1190. _H04
TTTGG TTTTAATTTTTCCCG GGGAGGACGAUGCGGUU UGGUUUUAAUUUUUCCCGCAGACGACUCGCCCGA 1191. _H04
TATTGTTCATGTTTI'CTCTG GGGAGGACGAUGCGGUAUUGUUCAUGUU UUCUCUGCAGACGACUCGCCCGA 1192. .H04
TTATT'riTrGTTTGTTGTG GGGAGGACGAUGCGGUUAU UU UUUGUUUGU UGUGCAGACGACUCGCCCGA 1193. _H04
TTTG G GGTTTTCCCATG CCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCAUGCCCCAGACGACUCGCCCGA 1195. _H04
AATTATGTGTTTTGTGACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUGUGACCGCAGACGACUCGCCCGA 1196. _H04
TTATTTTTGTTACC G TTCCG GGGAGGACGAUGCGGUUAUUUUUGUUACCGUUCCGCAGACGACUCGCCCGA 1197 H04
tt in H4
HTS read variable region RNA sequences ft in H3 selection selection Selected sequenc
GGTGTGTTACCCTACTGCGC GGGAGGACGAUGCGGGGUGUGUUACCCUACUGCGCCAGACGACUCGCCCGA 1198_ _H04
TACTTTCCTTTTG CTCTCCG GGGAGGACGAUGCGGUACU UUCCUUUUGCUCUCCGCAGACGACUCGCCCGA 1199. _H04
TTTTTCCATTTATTTCGCCG GGGAGGACGAUGCGGUUU UUCCAUUUAU UUCGCCGCAGACGACUCGCCCGA 1200. .H04
CTATACTCTTCTCTTTT'CCG GGGAGGACGAUGCGGCUAUACUCUUCUCUUUUCCGCAGACGACUCGCCCGA 1201. _H04
TTTTAC7TGTTTTTTTGTCCG GGGAGGACGAUGCGGU UUUACUUG UUU UUUUG UCCGCAGACGACUCGCCCGA 1202_ .H04
TTCTATTTTC GTTTCTT C CG GGGAGGACGAUGCGGU UCUAUUUUCGUUUCUUCCGCAGACGACUCGCCCGA 1203. _H04
TTTTCTCATTACATTTTCCG GGGAGGACGAUGCGGUUUUCUCAUUACAUUUUCC6CAGACGACUCGCCCGA 1204. _H04
ACTTTTTATTCTTTCTACTG GGGAGGACGAUGCGGACUU UUUAUUCUU UCUACUGCAGACGACUCGCCCGA 1205. .H04
ACTTACTGTTTTCTTTTCCG GGGAGGACGAUGCGGACUUACUGUUUUCU UUUCCGCAGACGACUCGCCCGA 1206. ,H04
ATTTCTTTCTTTTTCCTCTG GGGAGGACGAUGCGGAU UUCUUUCUUUUUCCUCUGCAGACGACUCGCCCGA 1207. ,H04
CTTTTTTCTGTTTTTATCCG GGGAGGACGAUGCGGCUUUUUUGUGUUUUUAUCCGCAGAGGACUCGCCCGA 1208. _H04
TTCTTTG CTTTTATTTTCCC GGGAGGACGAUGC6GUUCUUUGCUUUUAUUUUCCCCAGACGACUCGCCCGA 1209, _H04
ATTCG TTTTTTTCTTG TTC CG GGGAGGACGAUGCGGAUUCG UUUUUUUCUUGUUCCGCAGACGACUCGCCCGA 1210:. .H04
TTTTCCTTC G CT CTTTCCG GGGAGGACGAUGCGGUUUUCCUUCGCUUCUUUCCGCAGACGACUCGCCCGA 1211. _H04
TCTCATCTACTTTTCTCCG GGGAGGACGAU6CG6UCUCAUCUACUUUUCUCCGCAGACGACUCGCCCGA 1212. .H04
GTATTTCTCTTTTCTCTCCG GGGAGGACGAUGCGGGUAUUUCUCUUUUCUCUCCGCAGACGACUCGCCCGA 1213. _H04
TCTTTTGTTATTCTTGTCCC GGGAGGACGAUGCGGUCUU UUGUUAUUCUUG UCCCCAGACGACUCGCCCGA 1214, .H04
TTrcmATTCTACTCTccG GGGAGGACGAUGCGGUU UCUUUAUUCUACUCUCCGCAGACGACUCGCCCGA 1215„ _H04
TGTAGTGGAGGTGGTGTGCG GGGAGGACGAUGCGGUG UAGUGGAGGUGGUGUGCGCAGACGACUCGCCCGA 1216_ .H04
CTTCTACTCACTTTCTTCCG GGGAGGACGAUGCGGCU UCUACUCACUUUCUUCCGCAGACGACUCGCCCGA 1217. _H04
TATTTTGTTCTCTTTTTGCC GGGAGGACGAUGCGGUAUUUUGUUCUCUUUUUGCCCAGACGACUCGCCCGA 1218. _H04
ATTTCACGTTG Tl i 1 1 ! ACCG GGGAGGACGAUGCGGAUUUCACG UUGUUU UUUACCGCAGACGACUCGCCCGA 1219. .H04
CTTACTCTTACTTTTTACCG GGGAGGACGAUGCGGCUUACUCUUACU UUUUACCGCAGACGACUCGCCCGA 1220. .HQ4
TTTTTACTTTTTTCGTTCCG GGGAGGACGAUGCGGUUUUUACUUUU UUCGUUCCGCAGACGACUCGCCCGA 1222. _H04
TTTCATATTTCTTTCTTCGC GGGAGGACGAUGCGGUUUCAUAUUUCUUUCUUCGCCAGACGACUCGCCCGA 1223. .HQ4
TTCTCCG GGGAGGACGAUGCGGCUUUU UUCUUUUU UCUCCGCAGACGACUCGCCCGA 1224. .H04
CG TTTTCTTTGTTTTCCG GGGAGGACGAUGCGGCCUUU UUUCUUUGU UUUCCGCAGACGACUCGCCCGA 1225. .H04
err πΑΤΑθτττπτπ α GGGAGGACGAUGCGGCUUUUUAUACUUUUUUUCCGCAGACGACUCGCCCGA 1226. _H04
# m H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc
CTTGTTTGCTTTCTTCTCCG GGGAGGACGAUGCGGCUUGUUUGCUUUCUUCUCCGCAGACGACUCGCCCGA 1229. _H04
TTTGTTTTTGTG CTTTTCCC GGGAGGACGAUGCGGUUUGUUUU UGUGCU UUUCCCCAGACGACUCGCCCGA 1230. _H04
ATCTTTGTTCTCTATTACCG GGGAGGACGAUGCGGAUCU UUGUUCUCUAUUACCGCAGACGACUCGCCCGA 1231. _HQ4
CTTTCTACTTGTCTCACCG GGGAGGACGAUGCGGCUUUCUACUUQJ UCUCACCGCAGACGACUCGCCCGA 1232. .H04
TTCTTTTG CG CCTTTTTCCG GGGAGGACGAUGCGGU UCUUUUGCGCCUUUUUCCGCAGACGACUCGCCCGA 1233. .H04
ATTTATTTTCTTTTCAGCCG GGGAGGACGAUGCGGAUUUAU UUUCUUU UCAGCCGCAGACGACUCGCCCGA 1234. .HQ4
TTTTTCCTATT ATG CTTCCG GGGAGGACGAUGCGGUUUUUCCUAUUAUGCUUCCGCAGACGACUCGCCCGA 1235. „H04
TTTTTTGTCTATCTTTTCCG GGGAGGAC'GAUGCGGUUUU UUGUCUAUCUU UUCCGCAGACGACUCGCCCGA 1236, _H04
TTCTTTCTTTTTTCCTTCCG GGGAGGACGAUGCGGUUCU UUCUU UUUUCCUUCCGCAGACGACLICGCCCGA 1237. .H04
GGGAGGACGAUGCGGCUUUCAU UCCUUUUUUCCCGCAGACGACUCGCCCGA 1238. _H04
TTTCACAC I H i l l ! !TGCTG GGGAGGACGAUGCGGUUUCACACUUUU UUUUUGCUGCAGACGACUCGCCCGA 1239 , .H04
ATTTTTTCTAGTTTTTGCTG GGGAGGACGAUGCGGAU UUUUUCUAGUUUUUGCUGCAGACGACUCGCCCGA 1240. _H04
TTTTTCTTTTA A CTTTTC C G GGGAGGACGAUGCGGU UUUUCUUUUAACUUUUCCGCAGACGACUCGCCCGA 124i_ _H04
GT^CTTTTATTTGTTTTTCCC GGGAGGACGAUGCGGGUUCUUUUAUUUGUUUUUCCCCAGACGACUCGCCCGA 1242. _H04
1TATTTAAATTTTTGTTGTG GGGAGGACGAUGCGGUUAUUUAAAUUUU UGUUG UGCAGACGACUCGCCCGA 1243. _H04
TCTGTTGTGCTTGATATGCCC GGGAGGACGAUGCGGUCUGUUGUGCUUGAUAUGCCCCAGACGACUCGCCCGA 1244 _H04
TTTTCTTGTTTATTCCACCG GGGAGGACGAUGCGGUUUUCU UGUUUAUUCCACCGCAGACGACUCGCCCGA 1245. JH 04
TATACTTTTTTCTTCCG CCG GGGAGGACGAUGCGGUAUACUU UUUUCUUCCGCCGCAGACGACUCGCCCGA 1246. H04
TCTTT ATCATTCTG TTTCCG GGGAGGACGAUGCGGUCUUUAUCAU UCUGUUUCCGCAGACGACUCGCCCGA 1247. .H04
GTCTTTTCITGTTTTCTG CC GGGAGGACGAUGCGGGUCU UUUCUUGUU UUCUGCCCAGACGACUCGCCCGA 1248. _H04
TATTTTATTCTATCCCTCCG GGGAGGACGAUGCGG UAUUU UAUUCUAUCCCUCCGCAGACGACUCGCCCGA 1249. _H04
CTCTTTAACCTTTCTTTCCG GGGAGGACGAUGCGGCUCUUUAACCUUUCUUUCCGCAGACGACUCGCCCGA 1250. _H04
TTTCCG GTTCTT'tTTTTCCG GGGAGGACGAUGCGGUUUCCGGUUCUUUUUUUCCGCAGACGACUCGCCCGA 1251. .H04
ATTTCTTATTCCTTACCCCG GGGAGGACGAUGCGGAU UUCUUAUUCCUUACCCCGCAGACGACUCGCCCGA 1252. .H04
ACTTCTCTTTCCTATTACCG GGGAGGACGAUGCG6ACUUCUCUUUC UAUUACCGCAGACGACUCGCCCGA 1253. _HD4
CTTTCTTTCTCTTTTTCCCG GGGAGGACGAUGCGGCUUUCUUUCUCUUU UUCCCGCAGACGACUCGCCCGA 1254. .H04
G TTTACATTTTCTTCTTCCG GGGAGGACGAUGCGGGUUUACAUUUUCUUCUUCCGCAGACGACUCGCCCGA 1255. _H04
CTTrTTTTCCTTTATTTCCG GGGAGGACGAUGCGGCU UUUUU UCCUUUAUUUCCGCAGACGACUCGCCCGA 1256. .H04
# ill H4
HIS read variable region RNA sequences ft in H3 selection selection Selected sequenc
TTTTTTACGCCTTTTTCCCG GGGAGGACGAUGCGGUUUUUUACGCCUUUUUCCCGCAGACGACUCGCCCGA 1257. H04
TTATATTACTTTTCCTTCTG GGGAGGACGAUGCGGUUAUAU UACUUUUCCUUCUGCAGACGACUCGCCCGA 1258. H04
ATCCCTTTCTTTTTCCCCCG GGGAGGACGAUGCGGAUCCCUUUCUUUUUCCCCCGCAGACGACUCGGCCGA 1261. ,H04
AATTTTCTT GTTTG CTG CCC GGGAGGACGAUGCGGAAUUUUCUUGUUUGCUGCCCCAGACGACUCGCCCGA 1262_ _H04
TTTGGGAGTAGGGTGCTCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGUGCUCCGCAGACGACUCGCCCGA 1263. .H04
CTTT'CTTCT G ACTTTCTCCG GGGAGGACGAUGCGGCUUUCUUCUGACUUUCUCCGCAGACGACUCGCCCGA 1264. H04
TCTGTTTTTCTTCCTTTGCC GGGAGGACGAUGCGGUCUG UUUUUCUUCCUU UGCCCAGACGACUCGCCCGA 1265. .H04
ATCTTTAATACTTCTCTCCG GGGAGGACGAUGCGGAUCU U UAAUACUUCUCUCCGCAGACGACUCGCCCGA 1265. .H04
TTTCTATCTTCCTTACTCCG GGGAGGACGAUGCGGU UUCUAUCUUCCUUACUCCGCAGACGACUCGCCCGA 1268. .H04
TATTTTCTTTTTT'CGCTGCC GGGAGGACGAUGCGGUAUUUUCUU UUUUCGCUGCCCAGACGACUCGCCCGA 1259. _H04
CTTGCTTTTTCATTTTTCCG GGGAGGACOAUGCGGCU UGCUUUU UCAUUUUUCCGCAGACGACUCGCCCGA 1270. .H04
TTT TTACATTCTCTCTCTG GGGAGGACGAUGCGGUUUUUUACAUUCUCUCUCUGCAGACGACUCGCCCGA 1271_ H04
CTTTTCATACGCTTTTTCCG GGGAGGACGAUGCGGCUUUUCAUACGCUUUUUCCGCAGACGACUCGCCCGA 1272. HQ4
GCTATT'CTTTTCTTTTTCCC GGGAGGACGAUGCGGGCUAU UCUUUUCUUUUUCCCCAGACGACUCGCCCGA 1273. HQ4
TTTATTTTAGTCT rTCCCG GGGAGGACGAUGCGG UUUAUUUUAGUCUCUUCCCGCAGACGACUCGCCCGA 1274. .H04
TTCATTTTTGTTTTCTCCCG GGGAGGACGAUGCGGUUCAUUUUUGUUUUCUCCCGCAGACGACUCGCCCGA 1275. _H04
TTTACTTTTTTACG CTTTCCC GGGAGGACGAUGCGGUU UACUUUU UUACGCUUJCCCCAGACGACUCGCCCGA 1276. _H04
GTTTTCTACCTTCTTTACCG GGGAGGACGAUGCGGGU UU UCUACCUUCUUUACCGCAGACGACUCGCCCGA 1277. _HQ4
TTT6GGGTTTCCCGTGC.CG GGGAGGACGAUGCGGU U UGGGGUUU CCCG UGCCGCAG ACG ACU CGCCCGA 1278. .H04
TTTTTACCTTTTTCCTTCCG GGGAGGACGAUGC6GUUUU UACCUU UUUCCUUCCGCAGACGACUCGCCCGA 1279. _H0
TTCATCGCTGTTTTTCTCCG GGGAGGACGAUGCGGUUCAUCGCUGUUUU UCUCCGCAGACGACUCGCCCGA 1280. H04
ACTTTTTTTTGTCTTCTGCCG GGGAGGACGAUGCGGACUUU UU UUUGUCUUCUGCCGCAGACGACUCGCCCGA 1281. .H04
CGATTTTTCTTCTTTTGCGC GGGAGGACGAUGCGGCGAUU UUUCUUCUU UUGCGCCAGACGACUCGCCCGA 1282. _H04
TCTTTTTTCATTTCTCACCG GGGAGGACGAUGCGGUCUUU UUUCAUUUCUCACCGCAGACGACUCGCCCGA 1283. _H04
ATTTTTTCTCTT ATTTG CCG GGGAGGACGAUGCGGAU UUUUUCUCUUAUUUGCCGCAGACGACUCGCCCGA 1284. _H04
ATTTTTCCTTGTCTTATACCG GGGAGGACGAUGCGGAU UUUUCCU'UGUCUUAUACCGCAGACGACUCGCCCGA 1285. H04
TTCCTTTTACCTTTTCG CCG GGGAGGACGAUGCGGUUCCUUUUACCUUUUCGCCGCAGACGACUCGCCCGA 1286. _HQ4
ccTTTCTmcTCTAcrccG GGGAGGACGAUGCGGCCUUUCUUUUCUCUACUCCGCAGACGACUCGCCCGA 1287 H04
in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc
TTTCTACTTTTTCTTTTCCC GGGAGGACGAUGCGGUUUCUACUUUUUCUUU UCCCCAGACGACUCGCCCGA 1289. _HG4
TTTTTACCCTTTTTTGTCCG GGGAGGACGAUGCGGUUUUUACCCUU UUU UGUCCGCAGACGACUCGCCCGA 1290. _H04
CTTGTTTCCTTTTTTCTCCG GGGAGGACGAUGCGGCU UG UUUCCUUUU UUCUCCGCAGACGACUCGCCCGA 1291. .H04
TCTTTTTTTCATTTTTTCCG GGGAGGACGAUGCGGUCUUUUUUUCAUU UUUUCCGCAGACGACUCGCCCGA 1292_ .H04
TCTTATTCTTTCTTCTCCGC GGGAGGACGAUGCGGUCUUAUUCUUUCUUCUCCGCCAGACGACUCGCCCGA 1294. _HQ4
TTCTTTTTA ACTTACTT CCG GGGAGGACGAUGCGGUUCU UUUUAACUUACUUCCGCAGACGACUCGCCCGA 1295. _H04
TTTTTCTCTTTTTTTCC6CCG GGGAGGACGAUGCGGUUUUUCUCUUUUUUUCCGCCGCAGACGACUCGCCCGA 1296. „H04
i I ί 11 I ATTTGTTAGTTGTG GGGAGGACGAUGCGGUUUUUUAUUUGU UAG UUGUGCAGACGACUCGCCCGA 1297. „H04
TTTTTCACACATTATTTCCG G GG AGG ACG AUG CGGUUUU UCACACA UUAUU UCCG CAG ACG ACU CG CCCG A 1298. .H04
TTTGTTTATTCTTTTTTTCCG GGGAGGACGAUGCGGU UUG UUUAUUCUUU UUU UCCGCAGACGACUCGCCCGA 1299. .H04
TCCTGTCGTCTTC6TCCC GGGAGGACGAUGCGGUCCUGUCGUCUUCGUCCCCAGACGACUCGCCCGA 1300. _H04
TATTTTCTTCTCTCGGTCCG GGGAGGACGAUGCGGUAUUUUCUUCUCUCGGUCCGCAGACGACUCGCCCGA 1301. HQ4
ATCTCTTCTTTCTTACCCCG GGGAGGACGAUGCGGAUCUCUUCUUUCUUACCCCGCAGACGACUCGCCCGA 1304 .H04
•J\ TTTCTATTTCCTTTG CCCCG GGGAGGACGAUGCGGUUUCUAUUUCCUUUGCCCCGCAGACGACUCGCCCGA 1305. .H04
TTTATATTTTCTCG CCTCCG GGGAGGACGAUGCGGUUUAUAUUUUCUCGCCUCCGCAGACGACUCGCCCGA 1306. „H04
GCTTCTTCTTTCTTCATCCG GGGAGGACGAUGCGGGCUUCUUCUUUCUUCAUCCGCAGACGACUCGCCCGA 1307. .H04
TATCTTG TTACTTTTTC CCC GGGAGGACGAUGCGGUAUCU UGUUACUUUUUCCCCCAGACGACUCGCCCGA 1308. .H04
απακττττττ τασ GGGAGGACGAUGCGGCCUUG UCU UUUU UCUUCCGCAGACGACUCGCCCGA 1309. _H04
TTTCTCTTATTCTCCTTCCG GGGAGGACGAUGCGGUUUCUCU UAUUCUCCUUCCGCAGACGACUCGCCCGA 1310. _H04
TAGTGTTTTTTTTTTGTGCC GGGAGGACGAUGCG6UAGUGUUUUUUU UUUGUGCCCAGACGACUCGCCCGA 1311. _H04
ATTTCTATCGC TTTCCCG GGGA6GACGAUGCGGAU UUCUAUCGCUUUUUCCCGCAGACGACUCGCCCGA 1312. „H04
TCA' rrGTCTTTTATTCCG GGGAGGACGAUGCGGUCAUUUUGUCUUUUAUUCCGCAGACGACUCGCCCGA 1313. .H04
ATCTTTCTTTCTCTTACCG C GGGAGGACGAUGCGGAUCUUUCUUUCUCUUACCGCCAGACGACUCGCCCGA 1314. .H04
CTTTCTAATACTCTTCTCCG GGGAGGACGAUGCGGCU UUCUAAUACUCUUCUCCGCAGACGACUCGCCCGA 1315. ,H04
TTTTCTAGCTTTCCTTTCCG GGGAGGACGAUGCGGUUUUCUAGCUUUCCUUUCCGCAGACGACUCGCCCGA 1316. .H04
CTTTTATT'CTGTTTTCACCG GG 6AGG ACG A UGCGGCU UU U AU U UUG U U UU CACCGCAG ACGACUCG CCCG A 1317. _H04
TTTCACTATCTTTTCTCCCG GGGAGGACGAUGCGGUUUCACUAUCUUUUCUCCCGCAGACGACUCGCCCGA 1318. _H04
ATCCTTTTG TTTTCTTACCG GGGAGGACGAUGCGGAUCCUUUUGUUUUCUUACCGCAGACGACUCGCCCGA 1320. _H04
# in H4
HTS read variable region RNA sequences 3 selection sseelleeccttiioonn Selected sequenc
CCTTTTTCATTTTTATCCCG GGGAGGACGAUGCGGCCUL UUUCAUUUUUAUCCCGCAGACGACUCGCCCGA 1321. .H04
TTTGTTG TTGTTTTTTTTTCT6 GGGAGGACGAUGCGGU UUGUUGUUGUUUUUUUUUCUGCAGACGACUCGCCCGA 1322.. OA
TTTGGGATTTTCCCGTGCCC GGGAGGACGAUGCGGU UUGGGAUUUUCCCGUGCCCCAGACGACUCGCCCGA 1323, _H04
TTTTG TTTG CTTTG TCTCCG GGGAGGACGAUGCGGU UUUGU UUGCUUUGUCUCCGCAGACGACUCGCCCGA 1324. ,H04
ATTCATTC ! i I I M ACCCCG GGGAGGACGAUGCGGAUUCAUUCUUUUUUACCCCGCAGACGACUCGCCCGA 1325. .H04
CTTATTTCTTGCG G TTTGCG GGGAGGACGAUGCGGCU UAUUUCUUCCGGUU UCCGCAGACGACUCGCCCGA 1326. _H04
TATTTCTT TTACTTTTG CCC GGGAGGACGAUGCGGUAUU UCUUUUACUUUUGCCCCAGACGACUCGCCCGA 1327. „HG4
TTTTTCCTTTTAACTTTCCG GGGAGGACGAUGCGGUUUU UCCUUUUAACUU UCCGCAGACGACUCGCCCGA 1328. .H04
TTCCTTTATTTTTCTCG CCG GGGAGGACGAUGCGGUUCCUUUAUUUUUCUCGCCGCAGACGACUCGCCCGA 1329. .H04
TATTTTTCCACTGTTTTCCG GGGAGGACGAUGCGGUAUU UUUCCACUGU UUUCCGCAGACGACUCGCCCGA 1330. .H04
CG7TTTACTTATTCTTTCCG GGGAGGACGAUGCGGCGUUUUACUUAUUCUUUCCGCAGACGACUCGCCCGA 1331. .H04
TTCACTTCTTTATTCTC CCG GGGA6GACGAUGCGGUUCA.CUUCUUUAUUCUCCCGCAGACGACUCGCCCGA 1333. _H04
TTTTTTCATCCTTTTGTCTG G6GAGGAC6AUGCGG UUUUUUCAUCCUUUUG UCUGCAGACGACUCGCCCGA 335 _H04
TTTCCTACTTTTTTCTTCCG GGGAGGACGAUGCGGUUUCCUACUUUUUUCUUCCGCAGACGACUCGCCCGA 1336. .H04
ACTTT1 TATTCI TTCCG GGGAGGACGAUGCGGACUUUUU UAUUCUU UU UCCGCAGACGACUCGCCCGA 1337. .H04
TTATTCTTAATTCTTTTCCG GGGAGGACGAUGCGGUUAU UCU UAAUUCUUUUCCGCAGACGACUCGCCCGA 1338. _H04
TCTTTCTCTCCT CTTTG CCG GGGAGGACGAUGCGGUCUUUCUCUCCUCUU UGCCGCAGACGACUCGCCCGA 1339. _H04
A CTCTGGTTTCTTTCTCCG GGGAGGACGAUGCGGAOCUCUGGUUUCUU UCUCCGCAGACGACUCGCCCGA 1340. .H04
TTACTGCTTATTTCTCTCCG GGGAGGACGAUGCGGUUACUGCUUAUU UGUCUCCGCAGACGACUCGCCCGA 1341. ,H04
TTTTCTTTTCTGTTTATCCG GGGAGGACGAUGCGGUUUUCUUUUCUGUUUAUCCGCAGACGACUCGCCCGA 1342. H04
TTCTACTTCTTTCTTCACCG GGGAGGACGAUGCGGUUCUACUUCUUUCUUCACCGCAGACGACUCGCCCGA 1343. _H04
CTTTTTCGTTTTTTCCACCG GGGAGGACGAUGCGGCUUU UUCGUUUUUUCCACCGCAGACGACUCGCCCGA 1344. .H04
TTGTTCTTTCATT1TCTCC6 GGGAGGACGAUGCGGUUGUUCUUUCAUUUUCUCCGCAGACGACUCGCCCGA 1345. .H04
CTTTTAATTGTTCCCTTCCG GGGAGGACGAUGCGGCUUUUAAUUGUUCCCUUCCGCAGACGACUCGCCCGA 1346. _H04
A.CTATTTTCTGTTTTCACCG GGGAGGACGAUGCGGACUAUUUUCUGUUUUCACCGCAGACGACUCGCCCGA 1347. _HG4
CCTTATTTACTTTTACTCCG GGGAGGACGAUGCGGCCUUAUUUACUU UUACUCCGCAGACGACUCGCCCGA 1348 HD4
TTTTTCAGGTTTTTTTCGCAT GGGAGGACGAUGCGGUU UUUCAGGUU UUUUUCGCAUCAGACGAGUCGCCCGA 1349„H04
TCCTGTCGTCTTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCG UCU UGUUCGUCCCCAGACGACUCGCCCGA 1350 H04
# iti H4
HTS read variable region RNA sequences # irt H3 selection selection Seiected sequenc
TTTTATCACCTTCTTGTCCG GGGAGGACGAUGCGGUUUUAUCACCUUCUUGUCCGCAGACGACUCGCCCGA 1354 .H04
CTTTTACTTGTCTTTTACCG GGGAGGACGAUGCGGCUUUUACUUGUCUUUUACCGCAGACGACUCGCCC6A 1355. HQ4
TTATTTCTTT ATTTTCG CTG GGGAGGACGAUGCGGU UAUUUCUUUAUU UUCGCUGCAGACGACUCGCCCGA 1356, .H04
TCTTAATATGTTCTTCTCCG GGGAGGACGAUGCGGUCUUAAUAUGUUCUUCUCCGCAGACGACUCGCCCGA 1357_ .H04
CTTTCTTTTACTTTTCACCG GGGAGGACGAUGCGGCU UUCUUUUACUUUUCACCGCAGACGACUCGCCCGA 1358. .H04
TTTTTTTCCTATCTCTGTCCG GGGAGGACGAUGCGGU UUUUU UCCUAUCUCUGLCCGCAGACGACUCGCCCGA 1359. .H04
CTmCCTTTTTTTTGTTCCG GGGAGGACGAUGCGGCUUUUCCUUUUUUUUGUUCCGCAGACGACUCGCCCGA 136D. .H04
TTTCTCATTTTCATTATCCG GGGAGGACGAUGCGGUUUCUCAUUUUCAUUAUCCGCAGACGACUCGCCCGA 1361. „H04
CTCT TATTATTTTTCCCCG GGGAGGACGAUGCGGCUCUUUAUUAUU UUUCCCCGCAGACGACUCGCCCGA 1362. .H04
ATTCTTTCTTATTCTTGCTG GGGAGGACGAUGCGGAUUCUUUCUUAU UCUUGCUGCAGACGACUCGCCCGA 1363. .H04
TTCTTTGCTATCTTTTTCCG GGGAGGACGAUGCGGUUCU UUGCUAUCUUUUUCCGCAGACGACUCGCCCGA 1364. .H04
CCTTTACCTCTTTTATTCCG GGGAGGACGAUGCGGCCUUUACCUCUUUUAUUCCGCAGACGACUCGCCCGA 1365. _H04
CATTTACTTT CTTTTCACTG GGGAeGACGAUGCGGCAUUUACUUUCUUUUCACUGCAGACGACUCGCCCGA 1365. _H04
TCGTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCGUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1367. .H04
CTATTTTCTCTGTTCCTCCG GGGAGGACGAUGCGGCUAUUUUCUCUGUUCCUCCGCAGACGACUCGCCCGA 1369. _H04
TTGGGTGTAGGGGGGTTCCG GGGAGGACGAUGGGGUUGGGUGUAGGGGGGUUCCGCAGACGACUCGCCCGA 1370.. .H04
TTTTC ACTAC CCTTTCTCCG GGGAGGAC6AUGCGGUUUUCACUACCCUUUCUCCGCAGACGACUCGCCC6A 1371. _H04
TT6TTTCTCTTATTTCG CCC GGGAGGACGAUGCGGUUGU UUCUCUUAUUUCGCCCCAGACGACUCGCCCGA 1373. _H04
TTTACCTTTTCATCTCTCCG GGGAGGACGAUGCGGUUUACCUUUUCAUCUCUCCGCAGACGACUCGCCCGA 1374. _H04
TCTTTTATTTCATTCATCCG GGGAGGACGAUGCGGUCUUUUAUUUCAUUCAUCCGCAGACGACUCGCCCGA 1375. _H04
TTTTTCTCATCTTATCTCCG GGGAGGACGAUGCGG UUUUUCUCAUCUUAUCUCCGCAGACGACUCGCCCGA 1376. HOA
CTCTTTACTTTATCTTTCCG GGGAGGACGAUGCGGCUCUUUACUUUAUCU UUCCGCAGACGACUCGCCCGA 1377. HOA
TTTCTTTATTTCTCTTTG CG GGGAGGACGAUGCGGUUUCUUUAUUUCUCUUUGCGCAGACGACUCGCCCGA 13 9. HQ4
CTCTTAATG CTTTCTCTCCG GGGAGGACGAUGCGGCUCUUAAUGCUU UCUCUCCGCAGACGACUCGCCCGA 1380. HQ4
TTTACTTCTTTATATCTCCG GGGAGGACGAUGCGGU U UACUUCUUUAUAUCUCCGCAGACGACUCGCCCGA 1381. .H04
TTTCATATTTTTCTTCTCCG GGGAGGACGAUGCGGU U UCAUAUUUUUCUUCUCCGCAGACGACUCGCCCGA 1382. _H04
T ATTG CTATCTTTTTC C CCG GGGAGGACGAUGCGGUAUUGCUAUCUUUU UCCCCGCAGACGACUCGCCCGA 1383. _H04
ΤΑΤΤΪ ! I Π ! CCTTTTCTCCG GGGAGGACGAUGCGGUAUUUUUUUUCCUUUUCUCCGCAGACGACUCGCCCGA 1384. _H04
# in
HTS read variable region NA sequences in H3 selection selection Selected sequenc
ATTTTTTCCTATTTCTACCG GGGAGGACGAUGCGGAUUU UUUCCUAUU UGUACCGCAGAGGACUCGCCXGA 1385. _H04
TTATTGTTTTATrTTTTTGCC GGGAGGACGAUGCGGUUAU UGUUUUAUUU UUUUGCCCAGACGACUCGCCCGA 1386_ _H04
CTTTCTTTTTATACTTTCCG GGGAGGACGAUGCGGCUUUCUUUUUAUACUUUCCGCAGACGACUCGCCCGA 1387, .H04
TCCTCGTCGTCTTGCGTCGC GGGAGGACGAUGCCGUCCUCGUCGUCUUGCGUCCCCAGACGACUCGCCCGA 1388. _H04
TCTATTCATTCTCTTATCCG GGGAGGACGAUGCGGUCUAUUCAUUCUCUUAUCCGCAGACGACUCGCCCGA 1390. .H04
AACTTTTCCCTTTTC CACCG GGGAGGACGAUGCGGAACUUUUCCCUUUUCCACCGCAGACGACUCGGCCGA 1391. _H04
TTTCTTCTTGCTTCTTCCCG GGGAGGACGAUGCGGU UUCUUCUUGCUUCUUCCCGCAGACGACUCGCCCGA 1392, HQS,
ττττΑπετο6Ττ €τε€6 GGGAGGACGAUGCGGU UUUAU UCUCGUUUUCUCCGCAGACGACUCGCCCGA 1393,. _H04
fTFTCCTGTTC TACTCCG 6GGAGGACGAUGCGGUUUU UCCUGUUCUUACUCCGCAGACGACUC6CCCGA 1394. _H04
CTTTCTTATGCTCTTTACCG GGGAGGACGAUGCGGCU UUCUUAUGCUCUUUACCGCAGACGACUCGCCCGA 1395, _H04
TTTTTTCCTTTCACTATCCG GGGAGGACGAUGCGGUUUU UUCCUUUCACUAUCCGCAGACGACUCGCCCGA. 1396. _H04
TTCGTACTTTTTTTTTCCG GGGAGGACGAUGCGGUUCGUACUUUUUUUUUCCGCAGACGACUGGCCCGA 1397. „H04
AATCATTTCTTCTTCCACCG GGGAGGACGAUGCGGAAUCAUUUCUUCUUCCACCGCAGACGACUCGCGC6A 1398. .H04
TTTTI'CACTTTCACTTG CTG GGGAGGACGAUGCGGUUUUUCAC'UUUCACUUGCUGCAGACGACUCGCCCGA 1399. .H04
TTTATTTACTTTTCCCTCGC GGGAGGACGAUGCGGUUUAUUUACUUUUCCCUCGCCAGACGACUCGCCCGA 1400. .H04
CTTTACATTATCTCTTTCCG GGGAGGACGAUGCGGCU UUACAUUAUCUCU UUCCGCAGACGACUCGCCCGA 1401. _H04
TCTCTTTAAAATTCTCTCCG GGGAGGACGAUGCGGUCUCUUUAAAAUUCUCUCCGCAGACGACUCGCCCGA 1402. „H04
TCTT CTTTTACCCTATCCG GGGAGGACGAUGCGGUCUUUCUUUUACCCUAUCCGCAGACGACUCGCCCGA 1403. _H04
GTTTTATTTGTTGTGTTCCC GGGAGGACGAUGCGGGUUUUAUUUGUUGUGUUCCCCAGACGACUCGCCCGA 1404. .H04
C CTTTATTTC TTA7TCTC CG GGGAGGACGAUGCGGCCUUUAUUUCUUAUUCUCCGCAGACGACUCGCCCGA 1405. _H04
CTTTTTTCTTTTTCATTCCG GGGAGGACGAUGCGGCUUUUUUCUUUUUCAUUCCGCAGACGACUCGCCCGA 1406. _HQ4
TCTTATTCTTTTTTCCTCTG GGGAGGACGAUGCGGUCUUAUUCUUUUUUCCUCUGCAGACGACUCGCCCGA 1407. -H04
ATCTTTTCTGTCTTTTCCCG GGGAG6ACGAUGCGGAUCUU UUCUGUCU U UUCCCGCAGACGACUCGCCCGA 140S. .H04
TTTGTTGTGTTCTTTATCCG GGGAGGACGAUGCGGUUUGUUGUGUUCUUUAUCCGCAGACGACUCGCCCGA 1409. .H04
TTTGTC CTTG TTTTTG TC CG GGGAGGACGAUGCGGUUUGUCCUUGUUUUUGUCCGCAGACGACUCGCCCGA 1410. _H04
TATTTTTGCTTTCCTGTCCG GGGAGGACGAUGCGGUAUUUUUGCUUUCCUG UCCGCAGACGACUCGCCCGA 1411. _HQ4
TTTTGCTTATCTTCTGTCCG GGGAGGACGAUGCGGU UUUGCU UAUCUUCUG UCCGCAGACGACUCGCCCGA 1413. „H04
G ATACTTTTTTTTTTG CCCC GGGAGGACGAUGCGGGAUACUUUU UUUUUUGCCCCCAGACGACUCGCCCGA 1414 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CCTACTTTGTCTCTTTTCCG GGGAGGACGAUGCGGCCUACUUUGUCUCUUUUCCGCAGACGACUCGCCCGA 1415. _H04
GTTTTTTGATCTCTTGTCCC GGGAGGACGAUGCGGGUUUUUUGAUCUCUUGUCCCCAGACGACUCGCCCGA 1416. _H04
TOTCTnTTCTGnCTCCG GGGAGGACGAUGCGGUCUUCUUUU UCUGUUCUCCGCAGACGACUCGCCCGA 1417, „H04
GTTTTTATTTCCACTGTTCCC GGGAGGACGAUGCGGGUUUUUAUUUCCACUGUUCCCCAGACGACUCGCGCGA 1418. _H04
TTTATTTTACTGTTTCTCCG GGGAGGACGAUGCGGUUUAUUUUACUGUUUCUCCGCAGACGACUCGCCCGA 1419. H04
TTTGG I 1 1 t i 1 1 I I I GCCCG GGGAGGACGAUGCGGUUUGGU UU UUUUUUUGCCCGCAGACGACUCGCCCGA .1420. _H04
TTTCCACGGTTTTTTTTTCCG GGGAGGACGAUGCGGU UUCCACGGUUUUUUUUUCCGCAGACGACUCGCCCGA 1421. _H04
ATTTCTTCTCTTATTTCCCG GGGAGGACGAUGCGGAU UUCUUCUCUUAU UUCCCGCAGACGACUCGCCCGA 1422, JH04
TATTTTGATTTACTTTTCCC GGGAGGACGAUGCGGUAUUUUGAUU UACUUUUCCCCAGACGACUCGCCCGA 1423, „H04
GTTCTTTCATGTl rrccc GGGAGGACGAUGCGGGUUCUUUCAUGUUUUUUCCCCAGACGACUCGCCCGA 1424. .H04
GTTTGTTCTTTP TCCG GGGAGGACGAUGCGGGUUUGUUCUUUUUUUUUCCGCAGACGACUCGCCCGA 1426. .H04
TATTTACGTTTTTTTTTGTG GGGAGGACGAUGCGGUAUUUACG UUU UUUUUUGUGCAGACGACUCGCCCGA 1427. _HG4
AAGGAG6TGGTGTTATGGTG GGGAGGACGAUGCGGAAGGAGG UGGUGUUAUGG UGCAGACGACUCGCCCGA 1428_ _H04
TTATCAC CTTTCTTCTTCCG GGGAGGACGAUGCGGUUAUCACCUUUCUUCUUCCGCAGACGACUCGCCCGA 1429. .H04
TTTTCTTGGTCTTGTTGCCG GGGAGGACGAUGCGGUUUUCUUGGUCUUCUUGCCGCAGACGACUCGCCCGA 1430. _H04
TGCTTCTTTTTCTTTCCCCG GGGAGGACGAUGCGGUGCU UCU UUUUCUUUCCCCGCAGACGACUCGCCCGA 1431. _H04
TTTTCATTTCTCTTTTACCC GGGAGGACGAUGCGGUUUUCAUUUCUCUUUUACrXCAGACGACUCGCCCGA 1432. .H04
CTTCTCGTCTTTTCTTACCG GGGAGGACGAUGCGGCUUCUCGUCUUUUCUUACCGCAGACGACUCGCCCGA 1433. _H04
TTTTTCA6TGTTTTTGTGTG GGGAGGACGAUGGGGUUUU UCAGUGUUU UUGUGUGCAGACGACUCGCCCGA 1434. _HQ4
GTTTACTTTTTCCCTnTCC GGGAGGACGAUGCGGGU UUACUUUUUCCCUUUUCCCAGACGACUCGCCCGA 1435. .H04
CTTCTCTTACTTTTCTTCCG GGGAGGACGAUGCGGCUUCUCUUACUUUUCUUCCGCAGACGACUCGCCCGA 1436. _H04
CTTTTGTTTCTTCTTTCCCG GGGAGGACGAUGCGGCUUUUGU UUCUUCUUUCCCGCAGACGACUCGCCCGA 1437. _H04
TTTTCTTTTCTTGTTTTG CG GGGAGGACGAUGCGGUU UUCUUUUCUUGUU UUGCGCAGACGACUCGCCCGA 1438. .H04
ATTTTATTTTG TCTTTACCG GGGAGGACGAUGCGGAU UUUAU UUUGUCUUUACCGCAGACGACUCGCCCGA 1439, _HG4
TTTTTGCTTCTTTTTTTG CG GGGAGGACGAUGCGGUU UUUGCUUCUUUUU ULGCGCAGACGACUCGCCCGA 1440. , H04
TATTATCTTTTATTCTTGTG GGGAGGACGAUGCGGUAUUAUCUUUUAUUCUUGUGCAGACGACUCGCCCGA 1441. _H04
ATTCTCTTTCTTTTCACCG GGGAGGACGAUGCGGAUUCUCUU UCUUUUCACCGCAGACGACUCGCCCGA 1442. .H04
TACTTTCCCCTTTTTCTCCG GGGAGGACGAUGCGGUACUU UCCCCUUU UUCUCCGCAGACGACUCGCCCGA 1443. _H04
# in H4
HTS read variable region WA sequences # in H3 selection selection Selected sequenc
AGTTTCGTGTTTTTTTGCCC GGGAGGACGAU6CGGAGUU UCGUG U UUUUU UGCCCCAGACGACUCGCCCGA 1444 , .H04
TTATTCTTCGCCTTTCTCCG GGGAGGACGAUGCGGU UAU UCUUCGCCUUUCUCCGCAGACGACUCGCCCGA 1445. _H04
TTTATTCCACTTTCTTCCCG GGGAGGACGAUGCGGUUUAUUCCACUUUCUUCCCGCAGACGACUCGCCCGA 1446_ .H04
TTTGGGTGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGG UGUAGGGUGUUCCGCAGACGACUCGCCCGA 1447. .H04
CTTTTCTCTTTTTTTCC G CGC GGGAGGACGAUGCGGCUUUUCUCUUUUU UUCCGCGCCAGACGACUCGCCCGA 1449. J-f04
ATTTTTGCTCTTTCCTTCCG GGGAGGACGAUGCGGAU UUUUGCUCUUUCCUUCCGCAGACGACUCGCCCGA 1450_ _H04
CTTTCTTTATTTCCCTTCCG GGGAGGACGAUGCGGCU UUCUUUAUUUCCCUUCCGCAGACGACUCGCCCGA 1451 .H04
T ACTTTCG CTTTTCTTTCCG GGGAGGACGAUGCGGUACUUUCGCUUUUCUUUCCGCAGACGACUCGCCCGA 1452. _H04
CCTTTTCG TTTCCTTATCCG GGGAGGACGAUGCGGCCUJUUCG UUUCCUUAUCCGCAGACGACUCGCCCGA 1453_ _HQ4
TTTTTATACGTTTTGCTCCG GGGAGGACGAUGCGGUUUU UAUACGUUUUGCUCCGCAGACGACUCGCCCGA 1454 .H04
TTTC ACTG CTTCCTTTTCCG G6GAGGACGAUGCGGU U UCACUGCUUCCUU UUCCGCAGACG ACUCGCCCGA 1455. _H04
GTTTGCTTTTCTCTTTTCCG GGGAGGACGAUGCGGGU UUGCU UUUCUCUUU UCCGCAGACGACUCGCCCGA 1456. .H04
CCTTTACTCATTCTTTTCCG GGGAGGACGAUGCGGCCUUUACUCAUUCUU UUCCGCAGACGACUCGCCCGA 1457. _HQ4
ο
© πτταΑττοττταΑττττ α GGGAGGACGAUGCGGUUUUCAUUGUUUCAUUUUCUGCAGACGACUCGCCCGA 1458. _H04
TCTTTTTTTGTGTTTCTCCG GGGAGGACGAUGCGGUCUUUUUUUGUGUUUCUCCGCAGACGACUCGCCCGA 1459. -H04
TTGTTTTATTTTATTTGCCC GGGAGGACGAUGCGGUUGUUUUAUUUUAUU UGCCCCAGACGACUCGCCCGA 1460. ,H04
TTACCTTTTG ATTTTTTTCCC GGGAGGACGAUGCGGUUACCUUUUGAUUUUUUUCCCCAGACGACUCGCCCGA 1461. .H04
G TTACCTTTTCCTTTTTCCC GGGAGGACGAUGCGGGU UACC UUUUCCUUU UUCCCCAGACGACUCGCCCGA 1463. .H04
G TTTTCTCATTCTCTTA CCG GGGAGGACGAUGCGGGUUU LfCUCAUUCUCUUACCGCAGACGACUCGCCCGA 1464. .H04
ATTTTCCTCTTTACTTGCCG GGGAGGACGAUGC6GAUUUUCCUCUUUACUUGCCGCAGACGACUCGCCCGA 1465. .H04
ATTTTTCTTCTCTTAGCCCG GGGAGGACGAUGCGGAUUUU UCUUCUC'JUACCCCGCAGACGACUCGCCCGA 1467. .H04
CCTTTTACTTCCTTTTCCCG GGGAGGACGAUGCGGCCUUUUACU UCCU UUUCCCGCAGACGACUCGCCCGA 1468. _H04
AATTTTATTTTGTTGTACCG GGGAGGACGAUGCGGAAUUUUAU UUUGUUGUACCGCAGACGACUCGCCCGA 1469. _H04
TTCTTTGACTATTTACTCCG GGGAGGACGAUGCGGUUCUU UGACUAU UUACUCCGCAGACGACUCGCCCGA. 1470. _H04
TTTTCGTTTTTTTTTGCGCCG GGGAGGACGAUGCGG UU UUCGUUUUUUU UUGCGCCGCAGACGACUCGCCCGA 1471. .H04
TrrmTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUUUCUUUCCUUUGUCCGCAGACGACUCGCCCGA 1472. H04
A I G I T Π CTTCAT7TTACCG GGGAGGACGAUGCGGAUGUUUUCUUCAUUUUACCGCAGACGACUCGCCCGA 1473_ „H04
CTATTCAACTTCTTTTTCCG GGGAGGACGAUGCGGCUAUUCAACUUCUUUUUCCGCAGACGACUCGCCCGA 1475 H04
# in H4
HTS read variable region RNA sequences lection selection Selected sequenc TTTGTTTATTCTTTTTCCG GGGAGGACGAUGCGGU UUG UUUAUUCUUU UUCCGCAGACGACUCGCCCGA i476_HQ4
TTTCTTTG TGTTTTTATCCC GGGAGGACGAUGCGGU UUCUUUGUGUU UUUAUCCCCAGACGACUCGCCCGA 1477_H04
ATTTCACTACTACTTTACCG GGGAGGACGAUGCGGAULUCACUACUACUU UACCGCAGACGACUCGCCCGA 1478_H04
TTTTCTTTGTTTCTCG CCCG GGGAGGACGAU6CGGUUUUCUUUGUUUCUCGCCCGCAGACGACUCGCCCGA 1479„H04
TTCGTACm ί ! l ll! I CCG GGGAGGACGAUGCGGU UCGUACUUUUUU UUUUCCGCAGACGACUCGCCCGA 1482_H04
AATTTCTCGTTTTTTTTGCTG GGGAGGACGAUGCGGAAUU UCUCGUUUU UUUUGCUGCAGACGACUCGCCCGA 1483_H04
TTATCTTTTACTCTATTCCG GGGAGGACGAUGCGGUUAUCUUUUACUCUAU UCCGCAGACGACUCGCCCGA 1484_H04
TTTTAGCTTOT TTGTCCG GGGAGGACGAUGCGGUUUUAGCUUCUUUUUUGUCCGCAGACGACUCGCCCGA 1486. H04
GTTTTGTT1 CCTTTCCG GGGAGGACGAUGCGGG UUUUGUUUUUUUUCCUUUCCGCAGACGACUCGCCCGA 1487JH04
TTTTTTCTAATTTCTCCCCG GGGAGGACGAUGCGGUUUUUUCUAAUUUCUCCCCGCAGACGACUCGCCCGA 1488JHG4
AG IT CTTT ATTTT CTTTCCC GGGAGGACGAUGCGGAG UUCUU UAUUUUCUU UCCCCAGACGACUCGCCCGA 1489 . H04
CCTTATTT ATTTTCCTTCC G GGGAGGACGAUGCGGCCUUAUUUAUU UUCCUUCCGCAGACGACUCGCCCGA 1490„H04
TTCGGGGTTTTCCCGTGCCC GGGAGGACGAUGCGGU UCGGG6UUU UCCCGUGCCCCAGACGACUCGCCCGA 1491_H04
TCCTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1492_H04
TTAGGTGAAGGGTGGTTCCG GGGAGGACGAUGCGGUUAGGUGAAGGGUGG UUCCGCAGACGACUCGCCCGA 1493_H04
ATTCTTTATATCCCTTACCG GGGAGGACGAUGCGGAUUCUUUAUAUCCCUUACCGCAGACGACUCGCCCGA 1494_H04
GCTCTATTCCTTCTTTTCCG GGGAGGACGAUGCGGGCUCUAUUCCUUCUUUUCCGCAGACGACUCGCCCGA 1495_H04
TTTCTATGTCGTTTTGTCCG GGGAGGACGAUGCGGUUUCUAUGUCG UUUUG UCCGCAGACGACUCGCCCGA 1496„H04
GTTA CTCTTTTCCG CTTC C G GGGAGGACGAUGCGGGUUACUCUUUUCCGCUUCCGCAGACGACUCGCCCGA 1497_ HG4
ATGCTTGGAGTAGGGGGGTG GGGAGGACGAUGCGGAUGCUUGGAGUAGGGGGG UGC.AGAC6ACUCGCCCGA 1498_H04
TTATTTCTCCTTCG CTTC CG GGGAGGACGAUGCGGUUAUUUCUCCUUCGCUUCCGCAGACGACUCGCCCGA 1499_H04
TTTCTTTTCACCTTTACCCG GGGAGGACGAUGCGGUU UCUUUUCAeCUUUACCCGCAGACGACUCGCCCGA 1500_H04
TTTACTCTAGCTTTTCTCCG GGGAGGACGAUGCGGUU UACUCUAGCUUUUCUCCGCAGACGACUCGCCCGA 1501_H04
GTTTE'CTCTCTTCTTTGCC GGGAGGACGAUGCGGGUUUUCUCUCUUCUUUGCCCAGACGACUCGCCCGA 1502_H04
AGCTTTTCTCTTTCTTTCCG GGGAGGACGAUGCGGAGCUU UUCUCUUUCUUUCCGCAGACGACUCGCCCGA 1504_H04
CTTTAAACTTTTCTTTTCCG GGGAGGACGAUGCGGCU UuAAACUUUUCUUUUCCGCAGACGACUCGCCC A 15G5_ HQ4
CCTATTATTCATTTTCTCCG GGGAGGACGAUGCGGCCUAUUAU UCAUUUUCUCCGCAGACGACUC6CCCGA 1506_HQ4
CTCACTTCTTTCTTATTCCG GGGAGGACGAUGCGGCUCACUUCUUUCUUAUUCCGCAGACGACUCGCCCGA 1507 _H04
# in H4
HTS read variable region RNA sequences in H3 selection selection Selected sequenc ATCTTTCATTCTTCTTCCCG GGGAGGACGAUGCGGAUCUUUCAUUCUUeUUCCCGCAGACGACUCGCCCGA 1509. _H04
TTATTTATTTGTTCTTTGCC GGGAGGACGAUGCGGUUAUUUAUUUG UUCUUUGCCCAGACGACUCGCCCGA 1S10. _HG4
TTTTACTTTCTTACTTTG CC GGGAGGACGAUGCGGUUUUACUUUCUUACUU UGCCCAGACGACUCGCCCGA 1511. _H04
TTTA TTTTTG ACTTTTTCCG GGGAGGACGAUGCGGUUUAUUUUUGACUUU UUCCGCAGACGACUCGCCCGA 1512. _H04
TTCTA I I I 1 I I CTTCTTCCG GGGAGGACGAUGCGGUUCUAUUUUUUCUUCUUCCGCAGACGACUCGCCCGA 1513. _H04
TTTTATTTCTTCTTTTTCGC GGGAGGACGAUGCGGUUUUAUUUCUUCUUUUUCGCCAGACGACUCGCCCGA 1514. _H04
TTGTTTATTTTCTCTTTG CG GGGAGGACGAUGCGGUUGUUUAUUUUCUCU UUGCGCAGACGACUCGCCCGA 1515. .H04
GATCTCTTTTCCTCTACCCG GGGAGGACGAUGCGGGAUCUCUUUUCCUCUACCCGCAGACGACUCGCCCGA 1517. _HQ4
ATTTTCTCTCTTCTGCCCCG GGGAGGACGAUGCGGAUUUUCUCUCUUCUGCCCCGCAGACGACUCGCCCGA 1518. _H04
AGTGGAGAAGGGAGGTGCT6 GGGAGGAC6AUGCGGAGUGGAGAA6GGAGGUGCUGCAGACGACUCGCCCGA 1520. H04
TTCTTGTCTTTCTTTCCG GGGAGGACGAUGCGGUUCUUG UCUUUCUUUCCGCAGACGACUCGCCCGA 1521. .H04
TAG TGTTTT7TTTTGTG CC GGGAGGACGAUGCGGUAGUGUUUUUUU UUGUGCCCAGACGACUCGCCCGA 1523. _H04
TCmACGCTTTCTTCTCCG GGGAGGACGAUGCGGUCUU UACGCUUUCU UCUCCGCAGACGACUCGCCCGA 1524. .H04
ο
TTTTTCATGAACCCTTTCCG GGGAGGACGAUGCGGUUUUUCAUGAACCCUUUCCGCAGACGACUCGCCCGA 1525. _H04
ACTCTTTATCGTTTTTTCCG GGGAGGACGAUGCGGACUCU UUAUCGUUUUUUCCGCAGACGACUCGCCCGA 1526. _H04
eCTTTTTTTCTTTTCCACCG GGGAGGACGAUGCGGCCUUU UUUUCUUUUCCACCGCAGACGACUCGCCCGA 1527. .H04
TTTTTGTCGCTTTATTCCCG GGGAGGACGAUGCGGU UUUUG UCGCUUUAU UCCCGCAGACGACUCGCCCGA 1528, H04
TTTTTTCACTTTTCCCTC G GGGAGGACGAUGCGGUUUUUUCACUUUUCCCUCUGCAGACGACUCGCCCGA 1529. .H04
TTACTCTTTCTATTTCCCCC GGGAGGACGAUGCGGUUACUCUUUCUAUUUCCCCCCAGACGACUCGCCCGA 1530. _HQ4
ATTTTTATGTGTTGTTTGTG GGGAGGACGAUGCGGAUUU UUAUG UGUUGUUUGUGCAGACGACUCGCCCGA 1531. _H04
ATTCTTAATTTCTTTG CCCG GG GAG GACG A UG CG G AU UC U UA A U U U CU U UG CCCG CAG ACG AC U CGCCCG A 1532. _H04
TCTATTTTATTTGCTTTCCG GGGAGGACGAUGCGGUCUAU UUUAUUUGCUUUCCGCAGACGACUCGCCCGA 1533. _H04
TTTTCTTTCATCCTG GTCCG GGGAGGACGAUGCGGUUUUCUUUCAUCCUGGUCCGCAGACGACUCGCCCGA 1534 H04
ACCTTTTGTTTTCTATTCCG GGGAGGACGAUGCGGACCUUUUGUUUUCUAUUCCGCAGACGACUCGCCCGA 1535. _H04
ATTTTG TCTTTTTCTT C C G GGGAGGACGAUGCGGAUUUUGCUCU UUUUCUUCCGCAGACGACUCGCCCGA 1536. „H04
TTTTTGTCTACTTTTTCCGC GGGAGGACGAUGCGGUUUUUG UCUACUUUUUCCGCCAGACGACUCGCCCGA 1537. .H04
ATCTTTCTAAA' 'CACCG GGGAGGACGAUGCGGAUCUUUCUAAAUUUUCACCGCAGACGACUCGCCCGA 1538. _HQ4
TCTT 'CCTATTTCTCCG GGGAGGACGAUGCGGUCUUUUU UCCUAUUUCUCCGCAGACGACUCGCCCGA 1539. .H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
TTTTCATTATCTTTTTCCGC GGGAGGACGAUGCGGU UUUCAUUAUCUUUU UCCGCCAGAC6ACUCGCCCGA 1S42_H04
TTTGTTTTTCTTTTTTTCGCC GGGAGGACGAUGCGGUUUGUUUUUCUUUUUUUCGCCCAGACGACUCGCCCGA 1543. H04
TCCATGTTGTTTTTTTTTCCG GGGAGGACGAUGCGGUCCAUGUUGUU UUUUUUUCCGCAGACGACUCGCCCGA 1544_H04
TATTTACG TTTTTTTTTTG TG GGGAGGACGAUGCGGUAUU UACGU UUUUUU UU UGUGCAGACGACUCGCCCGA 1545_ H04
TCTTTTTTTTCACmCTCCG GGGAGGACGAUGCGGUCUUUUUUUUCACUUUCUCCGCAGACGACUCGCCCGA 1546_H04
TTT CCTTTTTATCTTTTC c c GGGAGGACGAUGCGGUUUCCUUUUUAUCUUUUCCCCAGACGACUCGCCCGA 1547JH04
TTTTTATGTTTTTTTCCGTG GGGAGGACGAUGCGCU UUUUAUGUUUUUUUCCGUGCAGACGACUCGCCCGA 1548_H04
TGmTTmGTTTCTTGTC GGGAGGACGAUGCGGUGUUAUUUUUGUUUCUUGUCCAGA GACUCGCCCGA 1549_H04
CAT C ACTTTTTCTTTACACCG GGGAGGACGAUGCGGCAUCACUUUUUCUUUACACCGCAGACGACUCGCCCGA 1551_H04
ATCTTTTTG TTCG CTTTCCG GGGAGGACGAUGCGGAUCUUU UUGUUCGCUUUCCGCAGACGACUCGCCCGA 1552_H04
CCTTT CTTCTTTTCCTT G CC GGGAGGACGAUGCGGCCUUUCUUCUUUUCCUUGCCCAGACGACUCGCCCGA 1553_H04
ATTTG TTCC ATTTTCC ACC G GGGAGGACGAUGCGGAUUUGUUCCAUUUUCCACCGCAGAGGACUCGCCCGA 1555_H04
T ACTCTTCTTTTTATCG CCG GGGAGGACGAUGCGGUACUCUUCUUUUUAUCGCCGCAGACGACUCGCCCGA 15S6_H04
ATCCTGTCTTATTTTCTCCG GGGAGGACGAUGCGGAUCCUGUCUUAUUUUCUCCGCAGACGACUCGCCCGA 1557_H04
TTTTCACATTATTTCCCCGC GGGAGGACGAUGCGGUUUUCACAUUAUUUCCCCGCCAGACGACUCGCCCGA 1558_H04
CTTTTTTTCCCTTGTTGTTGC GGGAGGACGAUGCGGCU UUUUUUCCCUUGUUGUUGCCAGACGACUCGCCCGA 1559_H04
TCTTTACTCATATTTTTCCG GGGAGGACGAUGCGGUCUUUACUCAUAUUUUUCCGCAGACGACUCGCCCGA 1560_H04
TTCTTTG TTTACCT!TCCCG GGGAGGACGAUGCGGUUCUUUGUUUACCU UUCCCGCAGACGACUCGCCCGA 1561_H04
I I I I I I CCTTTATTTTTGCC GGGAGGACGAUGCGGUUUUUUCCUUUAUUUUUGCCCAGACGACUCGCCCGA 1S63_H04
TTAGGIGAAGGGTGG7TTCG GGGAGGACGAUGCGGUUAGGUGAAGGGUGGUUUCGCAGACGACUCGCCCGA 1564_H04
ACTTTITCTTTTTTT CCCTG GGGAGGACGAUGCGGACUUUUUCUUUUUUUCCCUGCAGACGACUCGCCCGA 1565_H04
TTTTTTACTTCTCTTCG CTG GGGAGGACGAUGCGGUUUUUUACUUCUCUUCGCUGCAGACGACUCGCCCGA 156'6_H04
TTATTTACTITTTTTCTCTG GGGAGGACGAUGCGGUUAUUUACUUUUUUUCUCUGCAGACGACUCGCCCGA 1567_H04
TTTATTCTGTTTTTTTTTCCG GGGAGGACGAUGCGGUUUAU UCUGUUUUUUUUUCCGCAGACGACUCGCCCGA 1568_H04
CT FTCTATACATTTTTCCG GGGAGGACGAUGCGGCU UUUCUAUACAUUUUUCCGCAGACGACUCGCCCGA 1569_H04
TTATCTTACGTTTTTCTCTG GGGAGGACGAUGCGGUUAUCUUACGUUUUUCUCUGCAGACGACUCGCCCGA 157G_H04
TTTG TG I 1 1 I I I ATTTTCTG GGGAGGACGAUGCGGUU UGUG UUUUUUAUUUUCUGCAGACGACUCGCCCGA 1571_H04
ACACTTTTCTTTTCTTTCCC GGGAGGACGAUGCGGACACUU UUCUUU UCU UUCCCCAGACGACUCGCCCGA 1572 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc CTTTTATGTITCCCGTTCCG GGGAGGACGAUGCGGCUUUUAUGUUUCCCGU UCCGCAGACGACUCGCCCGA 1574_HQ4
ATTTTTTG ACTG TTTCCTC CG GGGAGGACGAUGCGGAUUU UU JGACUGUUUCCUCCGCAGACGACUCGCCCGA 1575„H04
TTCTTTTCTTTCTTCTCCG GGGAGGACGAUGCGGUUCUUUUCUUUCUUCUCCGCAGACGACUCGCCCGA 1577 H04
ACTTCTATTGCTCCTTTCCG GGGAGGACGAUGCGGACUUCUAUUGCUCCUUUCCGCAGACGACUCGCCCGA 1578_H04
GTTTGTTTCTTTGTTACCG GGGAGGACGAUGCGGG U'JUGUUUCUUUGUUACCGCAGACGACUCGCCCGA 1579_H04
ACTTTCACTTACTCTCACCG GGGAGGACGAUGCGGACUU UCACUUACUCUCACCGCAGACGACUCGCCCGA 1580_H04
GAATTATCCTG CATTCACCG G6GAGGACGAUGCGGGAAUUAUCCUGCAUUCACCGCAGACGACUCGCCCGA 1581_H04
TGCTTTGGAGTAGGGGGGTG GGGAGGACGAUGCGGUGCUUUGGAGUAGGGGGGUGCAGACGACUCGCCCGA 1583JH04
TTATTTTTG TTTCTCTTC CG GGGAGGACGAUGCGGUUAUUUUUGUUUCUCUUCCGCAGACGACUCGCCCGA 1584_H04
ATTTCAATTTG CTTTCACCG GGGAGGACGAUGCGGAUUUCAAUUUGCUUUCACCGCAGACGACUCGCCCGA 15S5_H04
TTCTTTTCTCTTTAACTCCG GGGAGGACGAUGCGGU UCUUU UCUCUUUAACUCCGCAGACGACUCGCCCGA 1586_H04
CATTCTTTTCTTTTTCGCTG GGGAGGACGAUGCGGCAUueuUUUCUUUUUCGCUGCAGACGACUCGCCCGA 1587_H04
TTATT ACTTTATTCTG TCCG GGGAGGACGAUGCGGUUAUUACUUUAUUCUGUCCGCAGACGACUCGCCCGA I588_H04
CATATCTTTTCATTTTCACCG GGGAGGACGAUGCGGCAUAUCUUUUCAUUUUCACCGCAGACGACUCGCCCGA 1590_H04
TTTTTCATACCTTTATTCCG GGGAGGACGAUGCGGUUUU UCAUACCUUUAU UCCGCAGACGACUCGCCCGA 1S91_H04
TTTCATCTTTTl rrrccG GGGAGGACGAUGCGGUUUCAUCUUUUUUUUUCCGCAGACGACUCGCCCGA 1592_H04
TTTTGTCT rCTGCAC GGGAGGACGAUGCGGUUUU UGLJCUUUUUUCUGCACCAGACGACUCGCCCGA 1593_ H04
ATTTTATCGCTTCTTTGCCG GGGAGGACGAUGCGGAUUU UAUCGCUUCUUUGCCGCAGACGACUCGCCCGA 1595_H04
ACTTCTTTTCTTCTTAACCG GGGAGGACGAUGCGGACUUCUU UUCUUCUUAACCGCAGACGACUCGCCCGA 1596._H04
TTTCTTTCATTTTTTTCCG GGGAGGACGAUGCGGUUUCUUUCAUUUU UUUCCGCAGACGACUCGCCCGA 1S97_H04
ACCGTTTCTTTTTCTTGCCG GGGAGGACGAUGCGGACCGU UUCUUUUUCUUGCCGCAGACGACUCGCCCGA 1S99_H04
TCCTGTCGCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGCUG UUCGUCCCCAGACGACUCGCCCGA 16O0_H04
ATCTCTTCTTTTTCCCCCCG GGGAGGACGAUGCGGAUCUCUUCUUUUUCCCCCCGCAGACGACUCGCCCGA 1601_H04
CCTTTTTACTATCCTCTCCG GGGAGGACGAUGCGGCCUUUUUACUAUCCUCUCCGCAGACGACUCGCCCGA 1602..H04
ττττΑπΰτττεςοοτοοα GGGAGGACGAUGCGGUUUUAU UCUUUCCGCUCCGCAGACGACUCGCCCGA 1603_H04
mrcAcmccTA^ccG GGGAGGACGAUGCGGUUUUCACUUUCCUACUUCCGCAGACGACUCGCGCGA 1604 H04
CTCACTTG CTCTTTTCTCCQ GGGAGGACGAUGCGGCUCACU UGCUCUUUUCUCCGCAGACGACUCGCCCGA ie05_H04
TATCTTCTCTACTCTACTCCG GGGAGGACGAUGCGGUAUCU UCUCUACUCUACUCCGCAGACGACUCGCCCGA 1607 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ACTTTCCATTACTTTTCCCG GGGAGGACGAUGCGGACUU UCCAUUACUUUUCCCGCAGACGACUCGCCCGA 16GS. H04
CCTTTACTTGTTTTTCACCG GGGAGGACGAUGCGGCCUUUACU UGUUUUUCACCGCAGACGACUCGCCCGA 1609. _H04
ATCTTACTAATTCCTCTCCG GGGAGGACGAUGCGGAUCU UACUAAU UCCUCUCCGCAGACGACUCGCCCGA 1611. .H04
ATTTTCATTTTTTTTCCGCCG GGGAGGACGAUGCGGAUUUUCAUUUUUUUUCCGCCGCAGACGACUCGCCCGA 1612. JH04
GTTTTCCTTCATTCTTTCCG GGGAGGACGAUGCGGGUUUUCCUUCAUUCUUUCCGCAGACGACUCGCCCGA 1613. _H04
ACTTTTCTTCGTTTTACCCG GGGAGGACGAUGCGGACUUUUCUUCGUUUUACCCGCAGACGACUCGCCCGA 1614. _H04
ATTCTCTTATTATCTTGCCG GGGAGGACGAUGCGGAU UCUCUUAUUAUCUUGCCGCAGACGACUCGCCCGA 1615. .H04
TCTTGTTTn'TTTTTTGGCCC GGGAGGACGAUGCGGUCUUGUUUUUUU UUUUGGCCCCAGACGACUCGCCCGA 1616. .H04
AATCTTCTCATTTCTTACCG GGGAGGACGAUGCGGAAUCU UCUCAUU UCUUACCGCAGACGACUCGCCCGA 1617. .H04
ATTTCTTCTTTTTGCCCCTG GGGAGGACGAUGCGGAUUUCUUCUUUUUGCCCCUGCAGACGACUCGCCCGA 1618. .H04
TTTTATGTCTT FGCTCCG GGGAGGACGAUGCGGUUUUAUGUCUUUUUGCUCCGCAGACGACUCGCCCGA 1619. .H04
GTTTTCT1 rcATrmccc GGGAGGACGAUGCGGGUUUUCUUUUCAU UUUUCCCCAGACGACUCGGCCGA 1621. _H04
TCTTTTTTTG TCTTTCTTCCG GGGAGGACGAUGCGGUCUUUUUUUG UCUUUCUUCCGCAGACGACUCGCCCGA 1622. _H04
ο
ACTTTTTTCTCTCTTTTCT G GGGAGGACGAUGCGGACUUU UUUCUCUCUUUUCUGCAGACGACUCGCCCGA 1623. .H04
! I I I I 1 AACTTCTTTTTCCG GGGAGGACGAUGCGGUUUUUUAACUUCUUUUUCCGCAGACGACUCGCCCGA 1625. _H04
ACTTTTCTTGTTTATTCTG GGGAGGACGAUGCGGACUUUUCUUGU UUAUUCUGCAGACGACUCGCCCGA 1626. .H04
TTA1 i l l I TATCCTTTTCCG GGGAGGACGAUGCGGUUAUUUUUUAUCCUUU UCCGCAGACGACUCGCCCGA 1627. _H04
TTCTTTATTTl TTCCCTCCG GGGAGGACGAUGCGGUUCUUUAUUUUUU UUCCCUCCGCAGACGACUCGCCCGA 1628. .H04
TCCTTTC G TTCTG CCTCCC GGGAGGACGAUGCGGUCCUUUCGUUCUGCCUCCCCAGACGACUCGCCCGA 1631. .H04
ATCTTTCTCTTTACTCTCCG GGGAGGACGAUGCGGAUCUUUCUCUUUACUCUCCGCAGACGACUCGCCCGA 1632. _H04
TTTATTTTTCATTCG CC CCG GGGAGGACGAUGCGGUUUAUUUUUCAUUCGCCCCGCAGACGACUCGCCCGA 1633. .H04
ACCG CTCTTTTCTTTTTCTCCG GGGAGGACGAUGCGGACCGCUCUUUUCUUUUUCUCCGCAGACGACUCGCCCGA 1634. _H04
TCnTTCATCTACTCATCCG GGGAGGACGAUGCGGUCUUUUCAUCUACUCAUCCGCAGACGACUCGCCCGA 1635. _H04
TTTCACTCTCTTTCACTCCG GGGAGGACGAUGCGGUUUCACUCUCUUUCACUCCGCAGACGACUCGCCCGA 1636. .H04
TCTTTTGTAATTGCTCTCCG GGGAGGACGAUGCGGUCUUU UGUAAU UGCUCUCCGCAGACGACUCGCCCGA 1637. .HD4
TTTGTTATTCATTTTTG CCC GGGAGGACGAUGCGGUUUGUUAUUCAUUUUUGCCCCAGACGACUCGCCCGA 1638. _H04
GTTCTCTTTTTATTCTG CCC GGGAGGACGAUGCGGGUUCUCUUUUUAUUCUGCCCCAGACGACUCGCCCGA 1639. _HD4
TCCGTTTCTTTCTTTATCCG GGGAGGACGAUGCGGUCCGUUUCUUUCUUUAUCCGCAGACGACUCGCCCGA 1640 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTCCAATGCTC7TTTCCG GGGAGGACGAUGCGGUUUUCCAAUGCUCU UUUCCGCAGACGACUCGCCCGA 1641_ HQ4
TTTGGGAGTATGGTGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAUGGUGUUCCGCAGACGACUCGCCGGA 16 2_H04
TATTCATCTTATTCTCTCCG GGGAGGACGAUGCGGUAUUCAUCUUAUUCUCUCCGCAGACGACUCGCCCGA 1644_H04
CTTTTAACATTCTCTTTCCG GGGAGGACGAUGCGGGUUUUAACAU UCUCUUUCCGCAGACGACUCGCCCGA 1645_H04
GATCTACTTTCTTTTCACCC GGGAGGACGAUGCGGGAUCUACUUUCUUUUCACCCCAGACGACUCGCCCGA 1646_H04
GTCTTTTGATTCTTTTGCCG GGGAGGACGAUGCGGGUCUUUUGAUUCU UUUGCCGCAGACGACUCGCCCGA 164?_H04
TTTTTTCTTTG CCCTTTCCG GGGAGGACGAUGCGGUUUU UUCUUUGCCCUU UCCGCAGAC6ACUCGCCCGA 1648_H04
TGTTTTTTATGCTTTTGCC6 GGGAGGACGAUGCGGUGUUUUUUAUGCUUU UGCCGCAGACGACUCGCCCGA 1650__H04
CTTTTATTTTTTCTTTG CCC GGGAGGACGAUGCGGCUUUUAUUUUUUCUUUGCCCCAGACGACUCGCCCGA 1651JH04
CTCTCTTTACA TTTCTCCG GGGAGGACGAUGCGGCUCUCUUUACAUUUUCUCCGCAGACGACUCGCCCGA 1652JHQ4
ATTTCTTAG CTTCCTTACCG GGGAGGACGAUGCGGAU UUCUUAGCUUCCUUACCGCAGACGACUCGCCCGA 1653JH04
CTTTTCACTTTTCTCTTCTG GGGAGGACGAUGCGGCUUUUCACUUUUCUCUUCUGCAGACGACUCGCCCGA 16S4_H04
TATCTTTAATTCTTTTTCCG GGGAGGACGAIJGCGGUAUCU UUAAUUCUUUUUCCGCAGACGACUCGCCCGA 1655_H04
ο
CTTTTCCTTATTCTTTACCG GGGA6GACGAUGCGGCU UUUCCUUAUUCUUUACCGCAGACGACUCGCCCGA 1656_H04
Al TTTAGTTCTGTTTCCG GGGAGGACGAUGCGGAUUU UUUUAGUUCUG UUUCCGCAGACGACUCGCCCGA 1657_ H04
TCI TCTAATTTTACCCCG GGGAGGACGAUGCGG UCUU U UCUAAUUUUACCCCGCAGACGACUCGCCCGA 1658_H04
TTTTCACTTACACTTTTCGC GGGAGGACGAUGCGGUUUUCACUUACACUUUUC6CCAGACGACUCGCCCGA 1659_H04
TTTTACACTTACTTTTTCGC GGGAGGACGAUGCGGUU UUACACUUACUU UUUCGCCAGACGACUCGCCCGA 1660_H04
TTTTTATCTCG TTTTTTCTG GGGAGGACGAUGCGGUU UUUAUCUCGUUUUU UCUGCAGACGACUCGCCCGA 1661_ H04
ATTATTTCTTTCTTTTACTG GGGAGGACGAUGCGGAU UAU UUCUUUCUUlJUAri lGCAGACGACUCGCCCGA 1662_H04
TTTTTCTATGTTCTTTTCGC GGGAGGACGAUGCGGUU UU UCUAUGUUCUUU DCGCCAGACGACUCGCCCGA 1663_H04
TTTTTTGTTTCTCTGTTGCTG 6GGAGGACGAUGCGGUUUUUUGUUUCUCUGUUGCUGCAGACGACUCGCCCGA 1G64_H04
TTTTCGAGTACCTTTTTCCG GGGAGGACGAUGCGGUUUUCGAGUACCUUUUUCCGCAGACGACUCGCCCGA 1665_H04
TTTTCGTTGTTTTTGTTTCG GGGAGGACGAUGCGGUUUUCGUUG UUUUUG UUUCGCAGACGACUCGCCCGA 1666_H04
TTTCTACTTTTTTATTGTCCG GGGAGGACGAUGCGGUU UCUACU UUUUUAUUGLCCGCAGACGACuCGCCCGA 166?_ H04
nAGTGTTTAGTTTTTTGTG GGGAGGACGAUGCGGUUAGUGUUUAGUU UUUUGUGCAGACGACUCGCCCGA 1668_H04
TATTTCACTTACTTTTTCTG GGGAGGACGAUGCGGUAUUUCACUUACUUUU'JCUGCAGACGACUCGCCCGA 1669_H04
TTTTCACTTCTTATTCCCCG GGGAGGACGAUGCGGUU UUCACUUCUUAUUCCCCGGAGACGACUCGCCCGA 1670 H04
# in H4
HTS read variable region A sequences # in H3 selection selection Selected sequenc
CG TTTCCTCT6 G CTCGTCCC GGGAGGACGAUGCGGCGUU UCCUCUGGCUCGUCCCCAGACGACUCGCCCGA 1671_ _H04
TTGTCGTCTGTTCGTCCC G6GAGGACGAUGCGGUUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1672. _H04
ATCTTACTCTCTTTTCGCCG GGGAGGACGAUGCGGAUCU UACUCUCUUU UCGCCGCAGACGACUCGCCCGA 1673_ .H04
CTTTGCTCTTTTTGTTACCG GGGAGGACGAUGCGGCUUUGCUCUUUUUGUUACCGCAGACGACUCGCCCGA 1675. _H04
TTTGGGAGTAGGGTGTTCTG GGGAGGACGAUGCGGUUUGGGAGUAGGGUG UUCUGCAGACGACUCGCCCGA 1677. _HQ4
GATTTCTTATTCCCTTTCCC GGGAGGACGAUGCGGGAUUUCUUAU UCCCUUUCCCCAGACGACUCGCCCGA 1678. _H04
TATCTTTTCTTTTTCCCCGG GGGAGGACGAUGCGGUAUCUUUUCUUUUUCCCCGGCAGACGACUCGCCCGA 1679. _H04
TrrCAAACTTTTACTTTCGG GGGAGGACGAUGCGGU UUCAAACUU UUACUUUCCGCAGACGACUCGCCCGA 1680. HG4
TCATTTATATTCTTCCTCCG GGGAGGACGAUGCGGUCAUUUAUAUUCUUCCUCCGCAGACGACUCGCCCGA 1681. _H04
CATTTCTTCTTTTCGTTCGC GGGAGGACGAUGCGGCAUUUCU UCU UUUCGU UCGCCAGACGACUCGCCCGA 1682„ .H04
AACTTTTTTCTTTTATGCCG GGGAGGACGAUGCGGAACUU UUUUCUU UUAUGCCGCAGACGACUCGCCCGA 1683. .H04
GTTTGCTC I ! i I ί I I CTGCCG GGGAGGACGAUGCGGG UUUGCUCUUU UUUUCUGCCGCAGACGACUCGCCCGA 1684. .H04
CTTTTCCACTATTCG TTCCG GGGAGGACGAUGCGGCUUU UCCACUAUUCGUUCCGCAGACGACUCGCCCGA 1685. _H04
ο
TTTTTTCATATTACGCTCCG GGGAGGACGAUGCGGUUUUUUCAUAUUACGCUCCGCAGACGACUCGCCCGA 1686. _H04
TTATTTTTTCTTCGCTG TCCG GGGAGGACGAUGCGGUUAUUUUUUCUUCGCUGUCCGCAGACGACUCGCCCGA 1687. JHQ4
ATTCTTCATCTTTTGCCCCG GGGAGGACGAUGCGGAUUCUUCAUCUUUUGCCCCGCAGACGACUCGCCCGA 1688. _H04
CTTCATTTATTTCTCCACCG GGGAGGACGAUGCGGCU UCAUUUAU UUCUCCACCGCAGACGACUCGCCCGA 1689. .H04
GCTTTCTTTCTTCTTTCCTG GGGAGGACGAUGCGGGCUUUCUUUCUUCUUUCCUGCAGACGACUCGCCCGA 1690. _H04
ATTCTCTTTTTCTCTAACCG GGGAGGACGAUGCGGAUUCUCUUUUUCUCIJAACCGCAGACGACUCGCCCGA 1691. _H04
CATTTCTTT ATTTCTTT CCG GGGAGGACGAUGCGGCAUUUCUUUAUUUCUU UCCGCAGACGACUCGCCCGA 1692. _H04
CTATTTCTAATCTCTCTCCG GGGAGGACGAUGCGGCUAUUUCUAAUCUCUCUCCGCAGAC.GACUCGCCCGA 1694. _H04
CCTACTTTTTTCTTTTTCCG GGGAGGACGAUGCGGCCUACUUUUUUCUUUUUCCGCAGACGACUCGCCCGA 1695. ,H04
ATTCTTTTGTT I TTTTCC CG GGGAGGACGAUGCGGAUUCU UUUG UUUUUU UCCCGCAGACGACUCGCCC6A 1696. _H04
CTTTTTTATTTCCCTTCCG GGGAGGACGAUGCGGCUUUUUUAU UUCCCUUCCGCAGACGACUCGCCCGA 1697. _H04
τττ Α€Α6ΑτττττοΓ εσ GGGAGGACGAUGCGGUUUUUACAGAUUUUUGCCCGCAGACGACUCGCCCGA 1698. .H04
TTTTATTGATTTTGTTTCCC GGGA6GACGAUGCGGU UUUAU UGAUUUUGUUUCCCCAGACGACUCGCCCGA 1700. _H04
TATTTCTCTATTTTCCTCTG GGGAGGACGAUGCGGUAUUUCUCUAUUUUCCUCUGCAGACGACUCGCCCGA 1701. _H04
TGTTTTCTTTCTTCATTCCG GGGAGGACGAUGCGGUGUU UUCUUUCUUCAU UCCGCAGACGACUCGCCCGA 1702 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc TTTATTTCTTTGCTCTTGCC GGGAGGACGAU6CGGUUUAUUUCUUUGCUCUUGCCCA.GACGACUCGCCCGA 1703_HQ4
ATTTTATTCCTTTTTTCCCG GGGAGGACGAUGCGGAUUU UAUUCCUUU UUUCCCGCAGACGACUCGCGCGA 1704_H04
TTTA I 1 1 1 1 1 Ή I CGCTCCG GGGAGGACGAUGCGGUUUAUUUUUUUUUCGCUCCGCAGACGACUCGCCCGA 1705 H04
TCTGAATAGGAGGAGGA6GC GGGAGGACGAUGCGGUCUGAAUAGGAGGAGGAGGCCAGACGACUCGCCCGA 1706_H04
TTTCG CTTTCATATTTTCCG GGGAGGACGAUGCGGU UUCGCUUUCAUAUUUUCCGCAGACGACUCGCCCGA 1707_H04
ACTTTTTG TTATTTTTTGC C GGGAGGACGAUGCGGACUUUUUGUUAUUUUUUGCCCAGACGACUCGCCCGA 1708_H04
TCCTGGCTTGTCGTTGCCCC GGGAGGACGAUGCGGUCCUGGCUUGUCGUUGCCCCCAGACGACUCGCCCGA 1709JH04
TCTATTCTTTTGCTcrrccG GGGAGGACGAUGCGGUCUAUUCUUUUGCUCUUCCGCAGACGACUCGCCCGA 171Q_H04
ATTCTTTGTATTTTTG CCCG GGGAGGACGAUGCGGAUUCUU UGUAUUUUUGCCCGCAGACGACUCGCCCGA 1711_H04
ATTTTATTCCGTTGTTGCCG GGGAGGACGAUGCGGAUUU UAUUCCCUUG UUGCCGCAGA.CGACUCGCCCGA 1712.. H04
ATCGCTCTATTTTTTCTCCG GGGAGGACGAUGCGGAUCGCUCUAUUUUU UCUCCGCAGACGACUCGCCCGA 1713_H04
CTCCTTGTTCTTTTCCTCCG GGGAGGACGAUGCGGCUCCUUGUUCUUUUCCUCCGCAGACGACUCGCCCGA 1714_H04
G G CTTTT ACCTTTTTT CCCC GGGAGGACGAUGCGGGGCUUUUACCUUUUUUCCCCCAGACGACUCGCCCGA 1715_H04
TATTTCTCTTAACTCCTCCG GGGAGGACGAUGCGGUAUUUCUCUUAACUCCUCC6CAGACGACUCGCCCGA 1717_H04
TCTTG 1 I ί I I I I I I N i GGCCC GGGAGGACGAUGCGGUCUUGUUU UUUUUUUUUGGCCCCAGACGACUCGCCCGA 1718...H04
CTTTTCTCTTTCACCTACCG GGGAGGACGAUGCGGCUUU UCUCUUUCACCUACCGCAGACGACUCGCCCGA 1719_H04
TTTTCTATGTTCTTTGTCCG GGGAGGACGAUGCGGUUUUCUAUCUUCUUUGUCCGCAGACGACUCGCCCGA 1720_H04
CTTTCTCAcccrrmTCCG GGGA6GAC6AUGCGGCU UUCUCACCCUU UUUUCCGCAGACGACUCGCCCGA 1721_H04
ΤΤΤΤΑπΑΑΑΤΕΧΟΪΤΤίΧβ GGGAGGACGAUGCGGUUUUAUUAAAUCCG UUUCCGCAGACGACUCGCCCGA 1722_H04
ATCTTCTTI'GTTTTTTG CTG GGGAGGACGAUGCGGAUCUUCUUUGUUUUUU6CUGCAGAC6ACUCGCCCGA 1723_ HQ4
AAAAAGGAGGAGGTGGTGTG GGGAGGACGAUGCGGAAAAAGGAGGAGGUGGUGUGCAGACGACUCGCCCGA 1 24JH04
TCTTTTTTTGTTTCCCCCG GGGAGGACGAUGCGGUCUUUUUUUGUUUCCCCCGCAGACGACUCGCCCGA 1725_H04
CCTTTUCCXrATCTTTCCG GGGAGGACGAUGCGGCCU UUUUCCUUAUCUUUCCGCAGACGACUCGCCCGA 1726_H04
ATTCTCTTTTTCTATTACCG GGGAGGACGAUGCGGAUUCUCUUUUUCUAUUACCGCAGACGACUCGCCCGA 1727_H04
G'I'TCATGTTTTCCTTTTCGTC GGGAGGACGAUGCGGGU UCAUGUUUUCCUUUUCG UCCAGACGACUCGCCCGA 1728_H04
ATTTTGTCTTTTTCCTTCTG GGGAGGACGAUGCGGAU UUUGUCUUUUUCCUUCUGCAGACGACUCGCCCGA 1729 H04
TTTCTGTTTTTTTATTCCCG GGGAGGACGAUGCGGUUUCUGUUU UUUUAU UCCCGCAGACGACUCGCCCGA 1730„H04
TcrrrcTTTTCTTATTTccG GGGAGGACGAUGCGGUCUUUCUUUUCUUAUUUCCGCAGACGACUCGCCCGA 1731. H04
U in H4
HTS read variable region RNA sequences ilectfon selection Selected sequenc ATTGGTGAAG6GTGGTT6CG GGGAGGACGAUGCGGAUUGGUGAAGGGUGGUUGCGCAGACGACUCGCCCGA 1732_ H04
GTrfTCTCTCTrATTTTCCG GGGAGGACGAUGCGGG UUUUCUCUCUUAUUUUCCGCAGACGACUCGCCCGA 1733 H04
TACTTATTTGCCTTTTTCCG GGGAGGACGAUGCGGUACU UAUUUGCCUU UUUCCGCAGACGACUCGCCCGA 1734_HQ4
CCTTTTTCATTATTCTTCCG GGGAGGACGAUGCGGCCUU U UUCAUUAUUCUUCCGCAGACGACUCGCCCGA 1735_H04
CATTTATTA TTCCCCG GGGAGGACGAUGCGGCAUUUAU UAUUUUUUCCCCGCAGACGACUCGCCCGA 1736_H04
AA T CCTTCT7TACCCG GGGAGGACGAUGCGGAAUUUUUCCUUCUUUACCCGCAGACGACUCGCCCGA 1737„H04
TTTTC TTTCTATTTTGCC GGGAGGACGAUGCGGU UUUCUUUUUCUAUU UUGCCCAGACGACUCGCCCGA I738„H04
TACTT CTTTCATTTTGCC GGGAGGACGAUGCGGUACUUU UCUUUCAUUUUGCCCAGACGACUCGCCCGA 1739_H04
TTTCAC'I H I ! l ACTTTCGC GGGAGGACGAUGCGGUUUCACUUUUUUACUU UCGCCAGACGACUCGCCCGA 1740_H04
TATTTCACTG TTTGCTG GGGAGGACGAUGCGGUAUUUCACUGUUUUUUUGCUGCAGACGACUCGCCCGA 1741_H04
TG rTTACTTTTCCCG GGGAGGACGAUGCGGUGUU UUUUUUACUUU UCCCGCAGACGACUCGCCCGA 1742_H04
TCTGGTTTCTTTTGTTTCCG GGGAGGACGAUGCGGUCUGGUUUCUUUUGUUUCCGCAGACGACUCGCCCGA 1746„H04
TATCTCACACTTTCTTTCCG GGGAGGACGAUGCGGUAUCUCACACUUUCUUUCCGCAGACGACUCGCCCGA 1747_H04
ATTCTCAATTTCTCTCGCCG GGGAGGACGAUGCGGAUUCUCAAUUUCUCUCGCCGCAGACGACUCGCCCGA 1748_H04
TTATTTTTCCTTTTTCTGCCG GGGAGGACGAUGCGGUUAUUU UUCCUUU UUCUGCCGCAGACGACUCGCCCGA 1749_H04
TTrCTCTTAATTTTCCCCCG GGGAGGACGAUGCGGU UUCUCUUAAUUUUCCCCCGCAGACGACUCGCCCGA 1750_H04
TCATCTTTACTCTTCATCCG GGGAGGACGAUGCGGUCAUCUUUACUCUUCAUCCGCAGACGACUGGCCCGA 1752_H04
TTTTGC ! I i I f I 1 I i CCCC GGGAGGACGAUGCGGUUUUGCUUUUUUUUUCCCCCAGACGACUCGCCCGA 1753_H04
TTTTTCATTCCCCTTTTtCG G GGAGGACGAUGCGGUU U U UCA U U CCCCU U UUCCG CAGACG ACU CG CCCGA 17S4_H04
CTmCCTTTTTTTCACTCCG GGGAGGACGAUGCG6CUUUUCCUUUUUUUCACUCCGCAGACGACUCGCCCGA 1755JH04
TCTTGTTTTTTTTTTG G CCC GGGAGGACGAUGCGGUCUUGUUUUUUUUUUGGCCCCAGACGACUCGCCCGA 1756JH04
TCACTTTTTTTCATCTCCG GGGAGGACGAUGCGGUCACUUUUUUUCAUCUCCGCAGACGACUCGCCCGA 1757_H04
T Π"ί ί ! S ATTTTCCTGGTTCCG GGGAGGACGAUGCGGUUUUUUUAUUUUCCUGGU UCCGCAGACGACUCGCCCGA 1758_H04
TTTTCTTG CCCTTTTTCCG GGGAGGACGAUGCGGUUUUCU UGCCCUUUUUCCGCAGACGACUCGCCCGA 1759_H04
TTTATGAGAGTAGGGTGGTG GGGAGGACGAUGCGGUUUAUGAGAGUAGGGUGGUGCAGACGACUCGCCCGA 1760 H04
ATTGTTTTATTTTCTCACCC GGGAGGACGAUGCGGAUUGUUUUAUUUUCUCACCCCAGACGACUCGCCCGA 1?61_HQ4
CTTTTACATTATTTTCTCCG GGGAGGACGAUGCGGCUUUUACAUUAUUUUCUCCGCAGACGACUC6CCCGA 1762„H04
TTGTTTCTACCTTTTTTTCCG GGGAGGACGAUGCGGUUGUUUCUACCUUUUUUUCCGCAGACGACUCGCCCGA 1763 H04
» in B4
HTS read variable region RNA sequences 3 selection selection Selected sequenc TTTATCTTTTTTTGTGTG GGGAGGACGAUGCGGUUUAUCUUUUUU UGUG UGCAGACGACUCGCCCGA 1764_H04
ATCT'iTCGCTTTl TTCCCG GGGAGGACGAUGCGGAUCUUUCGCUUUUUU UCCCGCAGACGACUCGCCCGA 1765„H04
CTTTCCTAATTTTCTCTCCG GGGAGGACGAUGCGGCUUUCCUAAUU UUCUCUCCGCAGACGACUCGCCCGA 1766_H04
CTGTTCTTTTCCTTTTACCC GGGAGGACGAUGCGGCUGUUCUUUUCCUUUUACCCCAGACGACUCGCCCGA 1 67_H04
TTTATTCTG l 1 1 I ϊ I ! CCG GGGAGGACGAUGCGGUUUAUUCUGUUUUUUUCCGCAGACGACUCGCCCGA 1768_H04
TTTTACTGTTTTTCTTTGCG GGGAGGACGAUGCGGUUU UACUGUUUUUCUUUGCGCAGACGACUCGCCCGA 1769_H04
CTTTTGTTTGTTTCTTTGCG GGGAGGACGAUGCGGCUUU UGU UUGUUUCUUUGCGCAGACGACUCGCCCGA 1771„H04
TTCT ATTTAATTFTTCTCCG GGGAGGACGAUGCGGU UCUAUUUAAUUUUUCUCCGCAGACGACUCGCCCGA 1772_H04
GTTTTCAAGACTTRTTTCCC GGGAGGACGAUGCGGGUUUUCAAGACUUUUUUCCCCAGACGACUCGCCCGA 1773_H04
TTTGTA TG 1 1 I I I I I ! CCG GGGAGGACGAUGCGGU UUGuAAUGUUUUUUU UCCGCAGACGACUCGCCCGA 1774_ H04
TTCGTACTTTTTTTTTTTCCG GGGAGGACGAUGCGGU UCGUACUUUUUUUU UUUCCGCAGACGACUCGCCCGA 1775_ H04
CTTTTTTTTCCTTTTTTTC CG GGGAGGACGAUGCGGCU UUUUUUUCCUUUUUU UCCGCAGACGACUCGCCCGA 1776_H04
ATCTTCATTATCTTTTTGCC GGGAGGACGAUGCGGAUCUUCAUUAUCUUUUUGCCCAGACGACUCGCCCGA 1777„H04
TTTTTGCACTTATTTTTCCG G6GAGGACGAUGCGGUUUUUGCACUUAUUUUUCC6CAGACGACUCGCCCGA 1778_H04
TATTTTGTCACTTTTTTTTCTG GGGAGGACGAUGCGGUAUU UUG UCACUUUUUUU UCUGCAGACGACUCGCCCGA 1779_H04
TGTTTTTTCTTTTCTGTCTG GGGAGGACGAUGCGGUGUUUUUUCUUUUCUGUCUGCAGACGACUCGCCCGA 1780_H04
TTTCGATTTTATTTTTTCCC GGGAGGACGAUGCGGUUUCGAUUU UAUUUU UUCCCCAGACGACUCGCCCGA i'781_H04
ATTACCTTTTTCCCTTTCCG GGGAGGACGAUGCGGAUUACCUUUUUCCCUUUCCGCAGACGACUCGCCCGA 1 83_H04
AGTTTTTTCCATTTTTGCCC G6GAGGACGAUGCGGAG UUUUUUCCAUUUUU6CCCCAGACGACUCGCCCGA 178 JH04
ACTACTTTTCTCTTTTTCCG GGGAGGACGAUGCGGACUACUUUUCUCUUUUUCCGCAGACGACUCGCCCGA 1785_H04
CATTTACTTTTTACCCTCCG GGGAGGACGAUGCGGCAUUUACUUUU UACCCUCCGCAGACGACUCGCCCGA 17S6JH04
TTTCCTTTTCCTTTTCCCGC GGGAGGACGAUGCGGU ULCCUUUUCCUUU UCCCGCCAGACGACUCGCCCGA 1787JHG4
GATTTTCTTCCTTTTTGCCG GGGAGGACGAUGCGGGAUUUUCUUCCUUUUUGCCGCAGACGACUCGCCCGA 1788_H04
TITCCTAAATTTTTCCTCCG GGGAGGACGAUGCGGUUUCCUAAAUUUUUCCUCCGCAGACGACUCGCCCGA 1789_H04
TCTCATCTATTTTTCTTCCG GGGAGGACGAUGCGGUCUCAUCUAUUUUUCUUCCGCAGACGACUCGCCCGA 1790_H04
ACTTCTCTATTCTTTTCCCG GGGAGGACGAUGCGGACUUCUCUAUUCUUUUCCCGCAGACGACUCGCCCGA 1791_HG4
TTTTTTIGATTCTTGTTTCCG GGGAGGACGAUGCGGUUUU UUUGAUUCUUG UU UCCGCAGACGACUCGCGCGA 1792JHC4
TTCTTTGTCTAT'fTrCCCGC GGGAGGACGAUGCGGUUCUUUGUCUAUU UUCCCGCCAGACGACUCGCCCGA 1793 H04
# 5n H4
HTS read variable region RNA sequences lection selection Selected sequenc
TCATTTTAATTl'G CTCTCCG GGGAGGACGAUGCGGUCAU UU UAAUUUGCUCUCCGCAGACGACUCGCCCGA 1794_H04
CTTTTAATCTCTTTTTACCG GGGAGGACGAUGCGGCUUUUAAUCUCUUUUUACCGCAGACGACUCGCCCGA 1796JH04
TTATTTGTGATTTTTTTCCG GGGAGGACGAUGCGGUUAUUUGUGAUUUUUUUCCGCAGACGACUCGCCCGA 1797 JH04
TTTCTCAG CTTTTTTTTCCG GGGAGGACGAUGCGGUUUCUCAGCUUUUUUUUCCGCAGACGACUCGCCC6A 1798_H04
GACTT ATTCTTTTCTTGCC GGGAGGACGAUGCGGGACUUUAUUCU UUUCUUGCCCAGACGACUCGCCCGA 1799_H04
TTTTACA I 1 M i l l I GCTG GGGAGGACGAUGCGGU UUUACAUU UUUUUUCGCUGCAGACGACUCGCCCGA 1800_H04
ATTTTTTTC CTGTTTTTGCCG GG6AGGACGAUGCGGAU UUUU UUCCUGUUU UUGCCGCAGACGACUCGCCCGA 1801_H04
TTTCTTTCATTTTTTTTTCCG GGGAGGACGAUGCGGUUUCUU UCAUUUUUUUUUCCGCAGACGACUCGCCCGA 1802„H04
ACTTTTT6CTTATTTACCCG GGGAGGACGAUGCGGACUUU UUGCUUAUUUACCCGCAGACGACUCGCCCGA 18D3_H04
TTTTGC I I Ί I I ί I I i I CCCC GGGAGGACGAUGCGGUUUUGCUUUU UUUUUUCCCCCAGACGACUCGCCCGA 1804_H04
ACTTTTTCTCTTTTCTACCG GGGAGGACGAUGCGGACUUUUUCUCUUUUCUACCGCAGACGACUCGCCCGA 1805JHG4
TTCG CTTAATTCTTTTCCC6 GGGAGGACGAUGCGGUUCGCUUAAU UCU U UUCCCGCAGACGACUCGCCCGA 1806 _H04
TTTTATTTATTTCTG TCCCG G6GA6GACGAUGCGGU UUUAU UUAUUUCUG UCCC6CAGACGACUCGCCCGA 1807_ HG4
TTTTCTTCAATATTTTCCCG G GG AGG ACG A UG CGG U U U U C U U CAA U AU U U U CCCG CAG ACGAC U CG CCCG A 1808_H04
TCTTATTATTTCTTTCGCGC GGGAGGACGAUGCGGUCUUAUUAUUUCUUUCCCGCCAGACGAC CGCCCGA 18G9_H04
TGTTTTTCTA I I ! I ! i TCCC GGGAGGACGAUGCGGUGUUUUUCUAUUUUUU'UCCCCAGACGACUCGCCCGA 1810_H04
ATTTTATTTCTCTTTTCCCG GGGAGGACGAUGCGGAUUUUAUUUCUCU'UUUCCCGCAGACGACUCGCCCGA 1811._H04
ATTCTTTTTTGTTTTTTG CC GGGAGGACGAUGCGGAUUCUUU UUUGUUUUUUGCCCAGACGACUCGCCCGA 1812__HG4
TCCTTGTCGTCTCG CGTCCC GGGAGGAC6AUGCGGUCCUUGUCGUCUCGC6UCCCCAGACGACUCGCCCGA 1,814 JH04
TTTGGGAGTAAGGTGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAAGGUGUUCCGCAGACGACUCGCCCGA 1816_H04
GTTCTTATCCTTCTG TTCCG GGGAGGACGAUGCGGGUUCU UAUCCUUCUGUUCCGCAGACGACLfCGCCCGA 1817_H04
TTTTTCG ATCTTTGTTTCCG GGGAGGACGAUGCGGUUUU UCGAUCUU UGUUUCCG'CAGACGACUCGCCCGA 1818_H04
ATTTCTTCACTTGCTCACCG GGGAGGACGAUGCGGAU UUCUUCACUUGCUCACCGCAGACGACUCGCCCGA 1819„H04
AACTCTCTCTTTiTCTG CCG GGGAGGACGAUGCGGAACUCUCUCUUUUUCUGCCGCAGACGACUCGCCCGA 1820_H04
GTTGTTCTTTTAC CTCCG G C GGGAGGACGAUGCGGGUUGUUCUUUUACCUCCGGCCAGACGACUCGCCCGA 1822_H04
TTCCACTTTTTTTTCTCCG GGGAGGACGAUGCGGUUCCACUU UUUUUUCUCCGCAGACGACUCGCCCGA 1823_H04
TCTATTCTCTATTTCTTCCG GGGAGGACGAUGCGGUCUAUUCUCUAU UUCUUCCGCAGACGACUCGCCCGA 1825_H04
CATTTCACTTCTTTTCGCTG GGGAGGACGAUGCGGCAU UUCACUUCUUUUCGCUGCAGACGACUCGCCCGA 1826 H04
# ίπ Η4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ACATTTCATTTTTTCCeeCG GGGAGGACGAUGCGGACAUUUCAUU UUUUCCCCCGCAGACGACUCGCCCGA 1827_HQ4
CTTCTATT TTTTCCATCCG GGGAGGACGAUGCGGCUUCUAUUAUUUUCCAUCCGCAGACGACUCGCCCGA 182S_H04
T ATTT CA CTG TTTTTTG CTG GGGAGGACGAUGCGGUAUUUCACUGUUUUU UGCUGCAGACGACUCGCCCGA 1829_ HG4
ACTTTCTCTCTCTCTTTCCG GGGAGGACGAUGCGGACUUUQJCUCUCUCUUUCCGCAGACGACUCGCCCGA 1830J-TO4
TCTTTACT TTACATCTCCG GGGAGGACGAUGCGGUCUUUACUUUUACAUCUCCGCAGACGACUCGCCCGA 1831_H04
ATI rATTCTTTGGTTCCCG GGGAGGACGAUGCGGAUUUUAUUCUU UGGUUCCCGCAGACGACUCGCCCGA 1832_H04
ACTCATTCATTTCTCTACCG GGGAGGACGAUGCGGACUCAUUCAUUUCUCUACCGCAGACGACUCGCCCGA 1833_HG4
CTTTTAAATTTCTCCTTCCG GGGAGGACGAUGCGGCUUUUAAAUUUCUCCUUCCGCAGACGACUCGCCCGA 1834_ H04
CCATTTTTTCTTTTCCTGCC GGGAGGACGAUGCGGCCAUU UU UUCUUUUCCUGCCCAGACGACUCGCCCGA 1835JH04
ATTTCTTCGACTTTCCT'CCG GGGAGGACGAUGCGGAUUUCUUCGACUUUCCUCCGCAGACGACUCGCCCGA 1836_H04
TTTCTACACTTCTTTTTCCG GGGAGGACGAUGCGGUUUCUACACUUCUUUUUCCGCAGACGACUCGCCCGA 1837_H04
ATTTTGTTATGTTTTTGCCA GGGAGGACGAUGCGGAU UU UGUUAUG U U U U UG CCACAG ACGACU CGCCCGA 1838_H04
TCTTTTTTTTG TTTTTCTGCCG GGGAGGACGAUGCGGUCUUUUUUUUGUUUUUCUGCCGCAGACGACUCGCCCGA 1839_H04
CCTTTCTTTCCATTTTTCCG GGGAGGACGAUGCGGCCUUUCUUUCCAUUUUUCCGCAGACGACUCGCCCGA 1840_H04
ATCTTTACATTCACTTACCG GGGAGGACGAUGCGGAUCUUUACAUUCACUUACCGCAGACGACUCGCCCGA 1841JH04
TTTCATACTCATTTTTTCCG GGGAGGACGAUGCGGUU UCAUACUCAU UUUUUCCGCAGACGACUCGCCCGA 1842__H04
CCTTTTCTACTTCTTATCCG GGGAGGACGAUGCGGCCU UUUCUACUUCUUAUCCGCAGACGACUCGCCCGA 184"5_H04
ACTTTTTATTTTTATCACCG GGGAGGACGAUGCGGACUUU UUAUUUUUAUCACCGCAGACGACUCGCCCGA 1846JH04
GCTTCTCTCG Π Π I "I CCCG GGGAGGACGAUGCGGGCUUCUCUCGUUUUUUCCCGCAGACGACUCGCCCGA 1S47J-I04
ACGTTTTCTTCTATTTACCG GGGAGGACGAUGCGGACGUU UUCU UCUAU UUACCGCAGACGACUCGCCCGA 1848_H04
TTTCATCTCACTTTTTCCCG GGGAGGACGAUGCGG UUUCAUCUCACUUUUUCCC6CAGACGACUCGCCCGA 1850_ H04
GTTGTTTGGTTTCTTTTCCG GGGAGGACGAUGCGGGUUGUUUGGU UUCUUUUCCGCAGACGACUCGCCCGA 1851_H04
TT TTCATTTCATCTCTCCG GGGAGGACGAUGCGGUUUUUCAUUUCAUCUCUCCGCAGACGACUCGCCCGA 1852_H04
TCATTTTCTGTTTTCTTCTG GGGAGGACGAUGCGGUCAUUUUCUGUUUUCUUCUGCAGACGACUCGCCCGA 1853_HQ4
TTCTTGTGTTTTTCTTTCTG GGGAGGACGAUGCGGUUCUUGUGU UUUUCUUUCUGCAGACGACUCGCCCGA 1854_H04
TTTTGTTTTTCTTTTGTTCC GGGAGGACGAUGCGGUUUUGUUUUUOJU UUGUUCCCAGACGACUCGCCCGA 1855_HG4
TTCI GTTATTTTTCCCCTG GGGAGGACGAUGCGGUUCUUGU UAUUUUUCCCCUGCAGACGACUCGCCCGA 1856_H04
GCTTTTCTTCCTTTTTTCCG GGGAGGACGAUGCGGGCUUUUCUUCCUUU UUUCCGCAGACGACUCGCCCGA 1857 H04
# in H
HTS read variable region RNA sequences in H3 selection selection Selected sequenc CCTTGTTTGTTT TTTTCCCG GGGAGGACGAUGCGGCCUUGUUUGUUUUUUUUCCCGCAGACGACUCGCCCGA 1858_ H04
GTTTCTTATC1 TTTTCCG GGGAGGACGAUGCGGGUUUCUUAUCUUUUUUUCCGCAGACGACUCGCCCGA 1859_ H04
ATT ATTTTCTCTATTT A CTG GGGAGGACGAUGCGGAUUAUUUUCUCUAUUUACUGCAGACGACUCGCCCGA 1860_H04
TCCTGTCGTCTGTGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUGUGUCCCCAGACGACUCGCCCGA 1861JH04
TCCTGTCGTCTCTCGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUCUCG UCCCCAGACGACUCGCCCGA 1863JH04
TCCTTTCGTTCTACCCTCCC GGGAGGACGAUGCGGUCCUUUCGUUCUACCCUCCCCAGACGACUCGCCCGA 1865„H04
TTTG G GAGTAG G GTTC C G GGGAGGACGAUGCGGU UUGGGAGUAGGGUUCCGCAGACGACUCGCCCGA 1866_H04
6TTGGTGTAGGGGGGTTCCC GGGAGGACGAUGCGGG UUGGUGUAGGGGGGUUCCCCAGACGACUCGCCCGA 1867_H04
TTTTGGTGTTTTCTATTGTG GGGAGGACGAUGCGGU UUUCG UGUU UUCUAULGUGCAGACGACUCGCCCGA 1SS8_H04
GCCTTTCGTTCTTG CCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUGCCCUCCCCAGACGACUCGCCCGA 1870_H04
TAAT CTTCTTTTTGCTCCC GGGAGGACGAUGCGGUAAUUCUUCUUUUUGCUCCCCAGACGACUCGCCCGA 1871_ H04
TCGTTG mCTTTTTTCCCC GGGAGGACGAUGCGGUCGUUGUUUCUUUUUUCCCCCAGACGACUCGCCCGA 1S72._H04
TTGTTTTTTCcrrrrcTCGC GGGAGGACGAUGCGGUUGUUUUU UCCUUUUCI ICGCCAGACGACUCGCCCGA 1873_H04
TCATTT' rCTTCCTATTCCG GGGAGGACGAUGCGGUCAUUUCUCUUCCUAUUCCGCAGACGACUCGCCCGA 1875_H04
CTTTTTTCTTCTCTTGTTCCG GGGAGGACGAUGCGGCUUU UUUCUUCUCUUGUUCCGCAGACGACUCGCCCGA 1876_H04
TTTCAC ! I I ! I t I I CCCCGC GGGAGGACGAUGCGGU UUCACUUUUUUUUCCCCGCCAGACGACUCGCCCGA 1877„H04
ATTTTTTCCC I i I I H CCG GGGAGGACGAUGCGGAU UUUUUCCCAUUUUUUCCGCAGACGACUCGCCCGA 1878_H04
CTTAATTTGCTTCCTTTCCG GGGAGGACGAUGCGGCU UAAUUUGCUUCCUUUCCGCAGACGACUCGCCCGA 1879_H04
GGTTTGTTTTTCTGTTG CCG GGGAGGACGAUGCGGGGUU UGUU UUUCUGU UGCCGCAGACGACUCGCCCGA 1880_HD4
GTTCTTGTACCTTTTTTCCG GGGAGGACGAUGCGGGUUCUUGUACCUUUUUUCCGCAGACGACUCGCCCGA 1881_H04
TCTTmcArrTTrcGTTccG GGGAGGACGAUGCGGUCUUUUUCAUU UUUCGUUCCGCAGACGACUCGCCCGA 1882JH04
TTATTTCATCTTCTTTTGTG GGGAGGACGAUGCGGUUAU UUCAUCUUCUUU UGUGCAGACGACUCGCCCGA 1883_ H04
GTTTACGTACC nTTACCG GGGAGGACGAUGCGGGUUUACGUACCUUUUUUUACCGCAGACGACUCGCCCGA 1884_H04
CTC" GTTCTTATTTCCG GGGAGGACGAUGCGGCUCUUUUGUUCUUAUUUCCGCAGACGACUCGCCCGA 1885„H04
TTTTTAATG ATTTGTTCCCG GGGAGGACGAUGCGG'JUUU UAAUGAUUUGUUCCCGCAGACGACUCGCCCGA 1S87_H04
TTTrTTAGATTTTTG CTCCC GGGAGGACGAUGCGGUUUUUUAGAUUUUUGCUCCCCAGACGACUCGCCCGA 188&_ H04
TTTCTCTTTATATCGTTCCG GGGAGGACGAUGCGGUUUCUCUUUAUAUCGUUCCGCAGACGACUCGCCCGA 1889_H04
ATTTTTTTCTTTTT'n'GCCG GGGAGGACGAUGCGGAU UUUUUUCUUUUUU UGCCGCAGACGACUCGCCCGA 1890 H04
9 in H4
HTS read variable region RNA sequences election selection Selected sequenc
6TTCTATACTTTTCCTTCCG GGGAGGACGAUGCGGG UUCUAUACUUUUCCUOCCGCAGACGACUCGCCCGA 1891_H04
GTTT6CTCAAC I 1 1 1 I i CCG GGGAGGACGAUGCG6G UU UGCUCAACU UU UU UCCGCAGACGACUCGCCCGA 1892_H04
CCATTTTA.AATTTCTCTCCG GGGAGGACGAUGCGGCCAUU UUAAAU U UCUCUCCGCAGACGACUCGCCCGA 1893 H04
TTTTATTCTTTTTCTCCCTG GGGAGGACGAUGCGGU UUUAU UCUUUUUCUCCCUGCAGACGACUCGCCCGA 1895_H04
ATTCTTCATCATTTCTTCCG GGGA'GGACGAUGCGGAUUCUUCAUCAUUUCUUCCGCAGACGACUCGCCCGA 1896JH04
AATATTACTTTTTTTCCCCG GGGAGGACGAUGCGGAAUAUUACUUUUUUUCCCCGCAGACGACUCGCCCGA 1897_H04
ACTTGTTCGTTTTTTTGCTG GGGAGGACGAUGCGGACUUGUUCGUUUU UU UGCUGCAGACGACUCGCCCGA 1898„HQ4
TTTTTCTAATTTCG CTTGTG GGGAGGACGAUGCGGU UUUUCUAAUUUCGCUUGUGCAGACGACUCGCCCGA 1899_H04
TTTnCTCTTGTTTATlGJG GGGAGGACGAUGCGGUUUUUCUCUUGUU UAUUGUGCAGACGACUCGCCCGA I900_H04
GCTTTTTCGTTCTT~TTCCG GGGAGGACGAUGCGGGCUUUU UCGUUCUU U UUCCGCAGACGACUCGCCCGA 1901_H04
CCATCTrrTTCTTTTTTCCG GGGAGGACGAUGCGGCCAUCUUUUUCUUUUUUCCGCAGACGACUCGCCCGA 1902_H04
TTTTTATTATTCCTGTTGTG GGGAGGACGAUGCGGU UUUUAUUAUUCCUGUUGUGCAGACGACUCGCCCGA 1903_H04
TTTTCTAATTACTTTTCCCC GGGAGGACGAUGCGGUUUUCUAAUUACUUUUCCCCCAGACGACUCGCCCGA 1904 _H04
TTTTTATCTTTCTTCTTCCG GGGAGGACGAUGCGGUUUUUAUCUUUCUUCUUCCGCAGACGACUCGCCCGA 1905_H04
TCCTGTCGTTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCG UUCUGUUCG UCCCCAGACGACUCGCCCGA 1906_H04
Π I I I I CCCCTTTCTTTCCG GGGAGGACGAUGCGGU UUU UUCCCCUUUCUUUCCGCAGACGACUCGCCCGA 1908_H04
CATTTGTTCTTATTCCTCCG GGGAGGACGAUGCGGCAUUUGUUCUUAUUCCUCCGCAGACGACUCGCCCGA 1909_H04
TCTCATRCACGCTTTCTCCG GGGAGGACGAUGCGGUCUCAUUGACGCUUUCUCCGCAGACGACUCGCCCGA 1910.. H04
TTCGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUCGGGAG'UAGGG UGUUCCGCAGACGACUCGCCCGA 1911_H04
GATTTCGCTCATTTTTTGCC GGGAGGACGAUGCGGGAUUUCGCUCAUUUUUUGCCCAGACGACUCGCCCGA 1912_H04
GTTACTTCCTTCTTTTCCCG GGGAGGACGAUGCGGGUUACUUCCUUCUUUUCCCGCAGACGACUCGCCCGA I913_H04
TTTACTCTCTCTTTTCTCCG GGGAGGACGAUGCGGUUUACUCUCUCUUU'JCUCCGCAGACGACUCGCCCGA 1914_K04
ACCTATTCTCTTTTTCACCG GGGAGGACGAUGeGGACCUAUUCUCUU UUUCACCGCAGACGACUCGCCCGA 1915 H04
TCGTGTTTATTTTTTTTCCG GGGAGGACGAUGCGGUCGUG UUUAUUUUUU UUCCGCAGACGACUCGCCCGA 1917_H04
TTTCTGTTCTTTCTTCACCG GGGAGGACGAUGCGGU UUCUG UUCUUUCU UCACCGCAGACGACUCGCCCGA 1918 H04
TCACTTTCGCA I i ! I i CTACCG GGGAGGACGAUGCGGUCACUUUCGCAUUUUUCUACCGCAGACGACUCGCCCGA 1919 H04
CTATCTTTTACTTCCCTCCG GGGAGGACGAUGCGGCUAUCUU UUACUUCCCUCCGCAGACGACUCGCCCGA 1920_H04
AATCCTTTTTCTTGTCACCG GGGAGGACGAUGCGGAAUCCUUUIJUCUUG UCACCGCAGACGACUCGCCCGA 1921 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTTTGTATTGCTTCTCCG GGGAGGACGAUGCGGUUUUU UGUAUUGCU UCUCCGCAGACGACUCGCCCGA 1922, .H04
ACATTCTCTTTTTTTTCACC6 GGGAGGACGAUGCGGACAUUCUCUUUUUUUUCACCGCAGACGACUCGCCC6A 1923. _H04
CTTTTCGTCTCTTTTATCCG GGGAGGACGAUGCGGCUUU UCG UCUCUUUUAUCCGCAGACGACUCGCCCGA 1924_ H04
TTTTTCTTTTATTTCCGCGC GGGAGGACGAUGCGGUUU UUCUUUUAUUUCCGCGCCAGACGACUCGCCCGA 1925. _H04
TTT'CATTCACG I 1 1 I I I CCG GGGAGGACGAUGCGGUUUCAUUCACGUUUUUUCCGCAGACGACUCGCCCGA 1926. .H04
AACTTTTTCTCTTTTTACCC GGGAGGACGAUGCGGAACUUUUUCUCUUUUUACCCCAGACGACUCGCCCGA 1927. .H04
TTTTCATCAATTTTTCTCCG GGGAGGACGAUGCGGU UUUCAUCAAUUUUUCUCCGCAGACGACUCGCCCGA 1928. _H04
TTATTCTTTTCTTATTCCCC GGGAGGACGAUGCGGU UAUUCUUUUCUUAU UCCCCCAGACGACUCGCCCGA 1929. HG4
TTTTTATTCTTTTACCCCCC GGGAGGACGAUGCGGU UUUUAUUCUUU UACCCCCCCAGACGACUCGCCCGA 1930. .H04
CATTTTCTACTTTTTTCCG GGGAGGACGAUGCGGCAUU UUCUACUUUUUUCCGCAGACGACUCGCCCGA 1931. H04
TCTTTCG C ACTATTTTTCCG GGGAGGACGAUGCGGUCUUUCGCACUAUUUUUCCGCAGACGACUCGCCCGA 1932. .H04
GTTTATTTATTGTTTTTCCG GGGAGGACGAUGCGGGUUUAUUUAUUGUUUUUCCGCAGACGACUCGCCCGA 1933. .H04
ATTTCACGTTG TTTTTTTACCG GGGAGGACGAUGCGGAUUUCACGUUGUUUUUUUACCGCAGACGACUCGCCCGA 1934. .H04
•J\ TATTTGTTCTTTTG TTGCG GGGAGGACGAUGCGGUAUUUG UUCUUUUGUUGCGCAGACGACUCGCCCGA 1935. .H04
T ATTTTTTTCCTTTTCTC C G GGGAGGACGAUGCGGUAUUUUUUUCCUUUUCUCCGCAGACGACUCGCCCGA 1936. .H04
TTCTTTATTTTTACCTTCGC GGGAGGACGAUGCGGU UCUUUAUUUUUACCU UCGCCAGACGACUCGCCCGA 1937. .H04
TTTTATGTTACCTTTTCCTG GGGAGGACGAUGCGGUUUUAUGUUACCUUUUCCUGCAGACGACUCGCCCGA 1938. _H04
TTTGTTTTCTCTTTTTCCG GGGAGGACGAUGCGGUUUG UUUUCUCUUUUUCCGCAGACGACUCGCCCGA 1939. .H04
TTTTTTTCTATTCCTTTCCG GGGAGGACGAUGCGGUUUU UUUCUAUUCCUUUCCGCAGACGACUCGCCCGA 1940. .H04
TTTTAT TTTTGCTTTTCCCA GGGAGGACGAUGCGGUUUUAUUUUUGCUUU UCCCACAGACGACUCGCCCGA 1941. .H04
TTTATTTTCTCTTTGTTGTG GGGAGGACGAUGCGGUUUAUUUUCUCUUUGUUGUGCAGACGACUCGCCCGA 1942. .H04
TCCTGTCGTTCTGCCCTCCC GGGAGGACGAUGCGGUCCUG UCGUUCUGCCCUCCCCAGACGACUCGCCCGA 1944. _H04
ACCTTACTATTTCCCTTCCG GGGAGGACGAUGCGGACCUUACUAUUUCCCUUCCGCAGACGACUCGCCCGA 1947. .H04
CArrTTTCTCTTTTTGTGCC GGGAGGACGAUGCGGCAUUUUUCUCUUUUUGUGCCCAGACGACUCGCCCGA 1948. .H04
TTTTTTG CCTTCTCTCTCCG GGGAGGACGAUGCGGUUUUUUGCCUUCUCUCUCCGCAGACGACUCGCCCGA 1949. .H04
AATTATG TGTTTTTTTTGTG A GGGAGGACGAUGCGGAAUUAUGUGUUUUUUUUG UGACAGACGACUCGCCCGA 1950. .H04
TTTATTTCTTTCCACGTTCCC GGGAGGACGAUGCGG UUUAUUUCUUUCCACGUUCCCCAGACGACUCGCCCGA 1951. .H04
TCTTTTCCAACTTTTTGCCG GGGAGGACGAUGCGGUCUUU UCCAACUUUU UGCCGCAGACGACUCGCCCGA 1952 H04
U in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ACTTTTTA C CTTTT C C ACTG GGGAGGACGAUGCGGACUU UUUACCUUUUCCACUGCAGACGACUCGCCCGA 1954. _H04
ATTTTGCCCAGTTTTTTACCG GGGAGGACGAUGCGGAUUUUGeCCAGUUUUUUACCGCAGACGACUCGCCCGA 1955, HO
CC'fTTCTG TTTTCCTCTCCG GGGA6GACGAUGCGGCCUUUCU6UUUUCCUCUCCGCAGACGACUCGCCCGA 1957^ _Η04
TACTTTTGTTnTfTTCTCCG GGGAGGACGAUGCGGUACUUUUCUUUUUUUUCUCCGCAGACGACUCGCCCGA 1958. .H04
TTTCATTCTCTTCTTCTCCG GGGAGGACGAUGCGGU UUCAUUCUCUUCU UCUCCGCAGACGACUCGCCCGA 1959. _H04
CCGTTTGATTTC CTTTTCCG GGGAGGACGAUGCGGCCGJUUGAUUUCCUUU UCCGCAGACGACUCGCCCGA 1960. .H04
TATTTACG !")"!"! "I'M I CACCG GGGAGGACGAUGCGGUAUUUACGUUU UUUUUCACCGCAGACGACUCGCCCGA 1961. JH04
TTTTTTGTGGTTTGTTTCCG GG6AGGACGAUGCGGUUUUUUGUGGUU UGUUUCCGCAGACGACUCGCCCGA 1962. _H04
GATTTGT CTTTT CTTTGGCC GGGAGGACGAUGCGGGAUUUGUCUUUUCUUUGGCCCAGACGACUCGCCCGA 1964. _H04
CTTCATTG TTTCTTTTCCG C GGGAGGACGAUGCGGCUUCAUUGUUUCUUUUCCGCCAGACGACUCGCCCGA 1965. .H04
CCATTTCTTTTTTCCTTCCG GGGAGGACGAUGCGGCCAUUUCU UUUUUCCUUCCGCAGACGACUCGCCCGA 1966. .H04
GGTTCTTTCTTCCTTTTCCG GGGAGGACGAUGCGGGGUUCUUUCUUCCUUUUCCGCAGACGACUCGCCCGA 1967. _H04
TTTTCATTTC ACTTCTTG CC GGGAGGACGAUGCGGUUUUCAUUUCACUUCUUGCCCAGACGACUCGCCCGA 1968. _H04
CTTTTCACTCTATCTTACCG GGGAGGACGAUGCGGCUUUUCACUCUAUCUUACCGCAGACGACUCGCCCGA 1369, _HQ4
TTTGGG AGTAGG GTGTCCCG GGGAGGACGAUGCGGUUUGGGAGUAGGG UGUCCCGCAGACGACUCGCCCGA 137G_ .H04
TATTTTTTGCGCTTTCTCCG GGGAGGACGAUGCGGUAUUUUUUGCGCUU UCUCCGCAGACGACUCGCCCGA 1971. „H04
TTTTCTTTGTTTATCTCCCG GGGA6GACGAUGCGGUU UUCUUUG UUUAUCUCCCGCAGACGACUCGCCCGA 1973.. H04
TTTATTATTTTTCGCTTCCA GGGAGGACGAUGCGGUU UAUUAUUUUUCGCU UCCACAGACGACUCGCCCGA 197.4. _H04
TTTTTCTTTTTCTACGTCCG GGGAGGACGAUGCGGUU UUUCUUU UUCUACGUCCGCAGACGACUCGCCCGA 1975. .H04
TAGTGTTTTTTTTTTTTGTGCC GGGAGGACGAUGCGGUAGUGUUUUU UUUUU UUGUGCCCAGACGACUCGCCCGA 1976. _H04
TATCTCTTG TACTTCTTCCG GGGAGGACGAUGCGGUAUCUCUUGUACUUCUUCCGCAGACGACUCGCCCGA 1977. _H04
TTTTTTTGTTCG CTGTTTCTG GG6AGGACGAUGCGGUUUUUU UGUUCGCUGUUUCUGCAGACGACUCGCCCGA 1978. ,H04
TTTTGTTCTTTTTTTCTTCCG GGGAGGACGAUGCGGUUUUGUUCUUUUUUUCUUCCGCAGACGACUCGCCCGA 1979. _H04
GTTGTTTCACTTTTTCTCCG GGGAGGACGAUGCGGGUUGUUUCACUUUUUCUCCGCAGACGACUCGCCCGA 1980. .H04
TTTTATTCCTTTCTTTTCTG GGGAGGACGAUGCGGUUUUAUUCCUUUCUUUUCUGCAGACGACUCGCCCGA 1981. .H04
GATTCTACTCTTTCTCCCCG GGGAGGACGAUGCGGGAUUCUACUCUUUCUCCCCGCAGACGACUCGCCCGA 1982. .H04
ATTGTTTCTA I 1 5 I I I I CCG GGGAGGACGAUGCGGAU UGUUUCUAUUUUUU UCCGCAGACGACUCGCCCGA 1983. HQA
CTTTCTCACTTTTTTTCCCG GGGAGGACGAUGCGGCUUUCUCACUUUUU UUCCCGCAGACGACUCGCCCGA 1984 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
ATTCCACGTTTTTATTACCG GGGAGGACGAUGCGGAUUCCACGUU UUUAUUACCGCAGACGACUCGCCCGA 19S5 _H04
TATTTACGTTTTTTTTTCACCG GGGAGGACGAUGCGGUAUU UACGUUUUUUUUUCACCGCAGACGACUCGCCCGA I986_HG4
nTATTACTGTCTTTTTCTG GGGAGGACGAUGCGGU UUAUUACUGUCU UUUUCUGCAGACGACUCGCCCGA 1987_N04
CCATTTG TTTTTTTTCTTCCG GGGAGGACGAUGCGGCCAUUUGUUUUUUUUCUIJCCGCAGACGACUCGCCCGA 1988_HG4
TTTTATCACTTCTTTCG CGT GGGAGGACGAUGCGGUUUUAUCACUUCUUUCGCGUCAGACGACUCGCCCGA 1989_H0
TTTTTACTCTGATTTTTCTG GGGAGGACGAUGCGGUUU UUACUCUGAUUUUUCUGCAGACGACUCGCCCGA 1990_.HG4
TCCCT I C6TC I ITCGTCCC GGGAGGACGAUGCGGUCCCUGUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 1991_H04
TCCTGGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGGUCGUCUGUUCG UCCGCAGACGACUCGCCCGA 1992_H04
TACTTTTGTTTGTTTTGCGC GGGAGGACGAUGCGGUACUUUUGUUUGUUUUGCGCCAGACGACUCGCCCGA 1994_H04
CACTTTGTTTATTCCCTCCG GGGAGGACGAUGCGGCACUUUGUUUAUUCCCUCCGCAGACGACUCGCCCGA 1995_H04
TCCCTTTTGTTTTTCCCCGC GGGAGGACGAUGCGGUCCCU UUUGUUUUUCCCCGCCAGACGACUCGCCCGA I996,_H04
TACTCTTTGTTCCCTTTCCG GGGAGGACGAUGCGGUACUCUU UGUUCCCUUUCCGCAGACGACUCGCCCGA 199?_H04
TI TCCTTTTTTTTTCCCATCCG GGGAGGACGAUGCGGUUUCCUUUUUUUUUCCCAUCCGCAGACGACUCGCCCGA 1998_ H04
TGTACTCACTTTTTTTGTCCG GGGAGGACGAUGCGGUGUACUCACUUUUUUUGUCCGCAGACGACUCGCCCGA 2000_H04
TCTTTTGTTATTTCCATCCG GGGAGGACGAUGCGGUCUUUUGUUAUUUCCAUCCGCAGACGACUCGCCCGA 2001„H04
CTTCTTCTCTCTTATCTCCG GGGAGGACGAUGCGGCUUCU UCUCUCUUAUCUCCGCAGACGACUCGCCCGA 2002„H04
ATTCTTTTATCCTTGTTCCG GGGAGGACGAUGCGGAUUCUUUUAUCCUUGU UCCGCAGACGACUCGCCCGA 2003_H04
TTTCACACTTTATTTTGCCG GGGAGGACGAUGCGGUUUCACACUUUAUUUUGCCGCAGACGACUCGCCCGA 2004_H04
TGTCTTTCTAGTTTTTTGCCG GGGAGGACGAUGCGGUGUCUU UCUAGUUUUUUGCCGCAGACGACUCGCCCGA 2005_H04
TTTGGTTTTTTTTTTTGCCCG GGGAGGAC6AUGCGGUUUG6UUUUUUUUU UUGCCGGCAGACGACUCGCCCGA 2006_H04
AGTATCTATCTTTTTTGCCG GGGAGGACGAUGCGGAG UAUCUAUCUUUU UUGCCGCAGACGACUCGCCCGA 2007„H04
ATCTCTCTAATTCTCTTCCG GGGAGGACGAUGCGGAUCUCUCUAAU UCUCUUCCGCAGACGACUCGCCCGA 2008_H04
CTTTTTATTATTTCTTGCCC GGGAGGACGAUGCGGCUUUU UAUUAUUUCUUGCCCCAGACGACUCGCCCGA 2010_H04
TTTTCATGACTTATTTTCCC GGGAGGACGAUGCGGUUUUCAUGACUUAUUU UCCCCAGACGACUCGCCCGA 2011 H04
CACCTTTTCTCTTTTTACCG GGGAGGACGAUGCGGCACCUUUUCUCUUUUUACCGCAGACGACUCGCCCGA 2012_H04
ATTTGCTACTTTTCTCACCG GGGAGGACGAUGCGGAUUUGCUACUUUUCUCACCGCAGACGACUCGCCCGA 2013_H04
ATTTCG CT A ATTTTTT AC C G GGGAGGACGAUGCGGAU UUCGCUAAUUUUUUACCGCAGACGACUCGCCCGA 2014_H04
TTTTTCTTTCGTTGCTTCTG GGGAGGACGAUGCGGUUUU UCUUUCGUUGCU UCUGCAGACGACUCGCCCGA 2015 H04
in H4
HTS read variable region RNA sequences selection Selected sequenc
TATTTACATTTTTCCTTGTG GGGAGGACGAUGCGGUAUUUACAUUUUUCCUUG UGCAGACGACUCGCCCGA 20.16„H04
TCTCTTTTG TTCTTTTTG CC GGGAGGACGAUGCGGUCUCUUUUGUUCUUUUUGCCCAGACGACUCGCCCGA 2Q17 H04
TrTTATl'CTCTTTTCGCCAC GGGAGGACGAUGCGGUUUUAUUCUCUUUUCGCCACCAGACGACUCGCCCGA 2018J4Q4
TACTTTTCTTTCTCTTTCTG GGGAGGACGAUGCGGUACUUUUCUUUCUCUUUCUGCAGACGACUCGCCCGA 2019_H04
CTTTTCATTTC I ! 1 t i l ! I CCG GGGAGGACGAUGCGGCUUU UCAUUUCUUUUU UUUCCGCAGACGACUCGCCCGA 2020J-104
TTTTTTTCCATTGTTTTCCG GGGAGGACGAUGCGGUUUUUUUCCAUUGUUUUCCGCAGACGACUCGCCCGA 2021JH04
TTTT G TTTTTTC G TTTG TG GGGAGGACGAUGCGGU UUUAAGUUUUUUCGUUUGUGCAGACGACUCGCCCGA 2Q22JHD4
TGGAGTGGGGAATCGTCTCC GGGAGGACGAUGCGGUGGAGUGGGGAAUCGUCUCCCAGACGACUCGCCCGA 2G23_H04
AATTTTGTTCTTTTTTTGCC GGGAGGACGAUGCGGAAUUUUGUUCUUUUUUUGCCCAGACGACUCGCCCGA 2024_H04
TTTTCATTCTTTTTTCCCC GGGAGGACGAUGCGGUUUUCAU UCUUUUU UCCCCCAGACGACUCGCCCGA 2025„H04
TATTTTATCATTTTTCTCCG GGGAGGACGAUGCGGUAUU UUAUCAUUUUUCUCCGCAGACGACUCGCCCGA 2026_H04
TTACTTTG τττττττ ι CTCCG GGGAGGACGAUGCGGUUACU UUGUUUUUUUUCUCCGCAGACGACUCGCCCGA 2G27_H04
TTTG G G TTTTCCCGTG CCC GGGAGGACGAUGCGGUUUGGG UUUUCCCGUCCCCCAGACGACUCGCCCGA 2030_H04
TTTGGGAGTTGGGTGTTCCG GGGAGGACGAUGCGGUU UGGGAGU UGGGUGUUCCGCAGACGACUCGCCCGA 2031_H04
TTTGGAAGTAGGGTGTTCCG GGGAGGACGAUGCGGU UUGGAAGUAGGGUGUUCCGCAGACGACUCGCCCGA 2Q33_ HQ4
TTATCTCT AG TTTTG CTCC G GGGAGGACGAUGCGGUUAUCUCUAGUUUUGCUCCGCAGACGACUCGCCCGA 2034_HQ4
ATTCTTCTATTATTGTTCCG GGGAGGACGAUGCGGAUUCUUQJAUUAUUGUUCCGCAGACGACUCGCCCGA 2O35_H04
CACTCTG TTTCCTTTCTCCG GGGAGGACGAUGCGGCACUCUGUU UCCU UUCUCCGCAGACGACUCGCCCGA 2036_H04
TTTGGGAGGAGGGGGTTCCG GGGAGGACGAUGCGGUUUGGGAGGAGGGGGUUCCGCAGACGACUCGCCCGA 2037_H04
TTTTTCG ACTTTCTG CTCCG GGGAGGACGAUGCGGUUUUUCGACUUUCUGCUCCGCAGACGACUCGCCCGA 2Q38_ H04
CGTTTTATTCTTTTTTCGCC GGGAGGACGAUGCGGCGUUU UAUUCUUUUUUCGCCCAGACGACUCGCCCGA 2039_HO4
TCATTTTCTACTTTTTCCCG GGGAGGACGAUGCGG'UCAUUUUCUACUUUUUCCCGCAGACGACUCGCCCGA 2040_H04
CCATT'iTTTCTTTCTTTCCG GGGAGGACGAUGCGGCCAUUU UUUCUU UCU UUCCGCAGACGACUCGCCCGA 20 1_H04-
TTTTCATTGCTCACTCTCCG GGGAGGACGAUGCGG UUUUCAUUGCUCACUCUCCGCAGACGACUCGCCCGA 2042_HQ4
CTGTATGTT6GTAGCCACGC GGGAGGAC6AUGC6GCUGUAUGUU6GUAGCCACGCCAGA.CGACUCGCCCGA 2043_H04
TTTCTTCTACTTCTCGTCCG GGGAG GACGAU GC6G U U UCU UCU -ACU U CUCG UCCGCA ACG ACUCGCCCGA 2044_ H04
GTTTACTTCTTCTTTCCCCG GGGAGGACGAUGCGGGUUUACUUCUUCUU UCCCCGCAGACGACUCGCCCGA 2050„HG4
TTTTATCCTTTATTTCCCTG GGGAGGACGAUGCGGUUUUAUCCUUUAUUUCCCUGCAGACGACUCGCCCGA 2051 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TGTTTATTATC TTCGC GGGAGGACGAUGCGGUGUUUAUUAUCUU UUUUCGCCAGACGACUCGCCCGA 2052. _H04
CT AG CTTCTTTT ACCG GGGAGGACGAUGCGGCU UUUUAGCUUCUU UUACCGCAGACGACUCGCCCGA 2053. .H04
GCCTTGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUGUUCUGCCCUCCCCAGACGACUCGCCCGA 2054. _H04
TTTTCTACTTTCCTATTGTG GGGAGGACGAUGCGGUUUUCUACUUUCCUAUUGUGCAGACGACUCGCCCGA 2055. .H04
ACTTGTTCG I I I I I ! I I GCTG GGGAGGACGAUGCGGACUUG UUC6U U UUUUU UGCUGCAGACGACUCGCCCGA 2056. .H04
ACTTTTTATCTTG CTTACCC GGGAGGACGAUGCGGACUUU UUAUCUUGCUUACCCCAGACGACUCGCCCGA 2057. .H04
TTTACTTTTTATTTTGCGCC GGGAGGACGAUGCGGUUUACUUUU UAUUUUGCGCCCAGACGACUCGCCCGA 2058. _H04
TTTTTACATG TTTTCTC CCG GGGAGGACGAUGCGGUUUUUACAUGUUUUCUCCCGCAGACGACUCGCCCGA 2059. . H04
TTTTACTTCTTTTTTTTCG CAT GGGAGGACGAUGCGGUU UUACUUCUU UUUUU UCGCAUCAGACGACUCGCCCGA 2060. .H04
ATTTATCTA ί ! I I M I GCTG GGGAGGACGAUGCGGAU UUAUCUAUUUUUUUGCUGCAGACGACUCGCCCGA 2061. ,HQ4
ATCATTTTCTATTCTTCCCG GGGAGGACGAUGCGGAUCAUUUUCUAUUCUUCCCGCAGACGACUCGCCCGA 2062. .H04
1TTCGTAATACCTTTTTCCG GGGAGGACGAUGCGGUU UCGUAAUACCUUUUUCCGCAGACGACUCGCCCGA 2063. .H04
TTTTTTG TACTTTCTTCCCG GGGAGGACGAUGCGGUU UUUUGUACUUUCUUCCCGCAGACGACUCGCCCGA 2064. _H04
TrTCTTTTCATTTTrrTTCCG GGGAGGACGAUGCGGUUUCUUU UCAU UUUUUUUCCGCAGACGACUCGCCCGA 2065. _H04
TTTCTATTATTTCTTATCTG GGGAGGACGAUGCGGUUUCUAUUAUUUCU UAUCUGCAGACGACUCGCCCGA 2066. .H04
ATTTCTTTTAATTCTTG CCC GGGAGGACGAUGCG6AUUUCUUUUAAUUCUUGCCCCAGACGACUCGCCCGA 2067. _H04
CTTTTCATTTATTTTACCG GGGAGGACGAUGCGGCUUUUCAU UUAUUU UACCGCAGACGACUCGCCCGA 2068. _H04
TTTTATTTTATGCGTTTCCC GG6AGGACGAUGCGG UUUUAUUUUAUGCGUUUCCCCAGACGACUCGCCCGA 2069. HQ4
TTTATCATCATTTaTTCCG GGGAGGACGAUGCGGUUUAUCAUCAUUUCUUUCCGCAGACGACUCGCCCGA 2070. .H04
CTA f I 1 1 I I CC I 1 1 ί I 1 CCG GGGAGGACGAUGCGGCUAUUUUUUCCUUUUUUCCGCAGACGACUCGCCCGA 2071. „H04
ATTTTTTCTATTTCTTACCG GGGAGGACGAUGCGGAU UUUUUCUAU UUCUUACCGCAGACGACUCeCCCGA 2072. _HQ4
TAGTG TT'f'Tl I ί 1 1 TTGTGCC GGGAGGACGAUGCGGUAGUGUUUUUUUUUUUGUGCCCAGACGACUCGCCCGA 2073. _HG4
6TCTTATCTTCTTTTTACCG GGGAGGACGAUGCGGGUCUUAUCUUCUUU'JUACCGCAGACGACUCGCCCGA 2074. .H04
CATTTTTTCATTTTTCTCCG GGGAGGACGAUGCGGCAUUUUUUCAUUUUUCUCCGCAGACGACUCGCCCGA 2075. .H04
TATTCTTTTATTCTCTTGTG GGGAGGACGAUGCGGUAUUCUUUUAU UCUCUUGUGCAGACGAC'JCeCCCGA 2076. _H04
TCACTACAATTTTCCCTCCG GGGAGGACGAUGCGGUCACUACAAUUUUCCCUCCGCAGACGACUCGCCCGA 2078. _H04
G ATA CTTTTTTTTTG CCCC GGGAGGACGAUGCGGGAUACU UUUUUUUUGCCCCCAGACGACUCGCCCGA 2079. .H04
ACCA nTTCTCTTTTTCCG GGGAGGAC6AUGCGGACCACUUUUCUCUUUUUCC6CAGACGACUCGCCCGA 2081 H04
# irs H4
HIS read variable region RNA sequences election selection Selected sequenc ACTCACTTTTTCTTCT7CCG GGGAGGACGAUGCGGACUCACUUUUUQJUCUUCCGCAGACGACUCGCCCGA 2082_H04
TCTTTTACTTGTCCACTCCG GGGAGGACGAUGCGGUCUU UUACU UGUCCACUCCGCAGACGACUCGCCCGA 2O83_H04
T T A TC TTCT T CAT T G CT CC G GGGAGGACGAUGCGGU UAUCUUCU UCAUUGCUCCGCAGACGACUCGCCCGA 2084„H04
GGCTTTTCTTCTTTTTGCCG GGGAGGACGAUGCGGGGCUUUUCUUCUUUU UGCCGCAGACGACUCGCCCGA 2085_H04
αττΓπ £τττττοτ6€ςο GGGAGGACGAUGCGGCUUUUUCUCUUUUUG UGGCGCAGACGACUCGCCCGA 20S6_H04
ATTTATTTTTTTG CG CCCCG GGGAGGACGAUGCGGAUUUAU UUUUUUGCGCCCCGCAGACGACUCGCCCGA 2087_H04
TTTTTCCTACACTTATTCCG GGGAGGACGAUGCGGU UUUUCCUACACUUAUUCCGCAGACGACUCGCCCGA 2088_H04
CCATCTCTTTTTTTCCTCCG GGGAGGACGAUGCGGCCAUCUCUUUUUUUCCUCCGCAGACGACUCGCCCGA 2089_H04
TTCTACTTTCTTTTCATCCG GGGAGGACGAUGCGGUUCUACU UUCUUUUCAUCCGCAGACGACUCGCCCGA 2090_H04
CTCATTAATTCTTTTCTCCG GGGAGGACGAUGCGGCUCAUUAAUUCUUUUCUCCGCAGACGACUCGCCCGA 2091„H04
CTTTTTTTC G CCTATTTCCG GGGAGGACGAUGCGGCU UUUU UUCGCCUAUU UCCGCAGACGACUCGC CGA 2G92_ H04
CTCTTTACTTTTCTTCTCCG GGGAGGACGAUGCGGCUCUUUACUUUUCUUCUCCGCAGACGACUCGCCCGA 2093_H04
TTTG GGCGTAGG GTGTTCCG GGGAGGACGAUGCGGUUUGGGCGUAGGGUG UUCCGCAGACGACUCGCCCGA 2094_H04
TTCCTTTTTC CTTTG CTCCG GGGAGGACGAUGCGGUUCCUU UUUCCUUUGCUCCGCAGACGACUCGCCCGA 2095„H04
TCATTATTCTTTCACATCCG GGGAGGACGAUGCGGUCAUUAUUCUUUCACAUCCGCAGACGACUCGCCCGA 2096„H04
TTTCTCACTAATCCTTT'CCG GGGAGGACGAUGCGGUUUCUCACUAAUCCUUUCCGCAGACGACUCGCCCGA 2097„H04
ATTCCACTTTTTTTCACCG GGGAGGACGAUGCGGAUUCCACU UUUUUUCACCGCAGACGACUCGCCCGA 2098_H04
CATTATTTCTTCTCGCTATCCG GGGAGGACGAUGCGGCAUUAUUUCUUCUCGCUAUCCGCAGACGACUCGCCCGA 2100_H04
ACT' TACATCATTTTTACCG GGGAGGACGAUGCGGACUUUACAUCAUUUUUACCGCAGACGACUCGCCCGA 210i_H04
ACCTTATTTCTTTTTTG CTG GGGAGGACGAUGCGGACCUUAUUUCUUUUUUGCUGCAGAGGACUCGCCCGA 2102_ 04
CTTTCACTTTTTTTCTCTCCT GGGAGGACGAUGCGGCUUUCACUUUUUUUCUCUCCUCAGACGACUCGCCCGA 2104_H04
TTTTCTTTTCTTCCTTTCTG GGGAGGACGAUGCGGUUUUCUUUUCUUCCUU UCUGCAGACGACUCGCCCGA 2105..H04
TTTTTCTGTTTCG TTTTCCG GGGAGGACGAUGCGGU UUUUCUGUUUCGUU UUCCGCAGACGACUCGCCCGA 210?_H04
TTT CTTTATTTTTTG TGC G C GGGAGGACGAUGCGGU UUCUUUAUUUUUUGUGCGCCAGACGACUCGCCCGA 2108_ H04
TTTCATCTTTTI'TTTTrCCG GGGAGGACGAUGCGGUUUCAUCUU UUUUUU UUCCGCAGACGACUCGCCCGA 2109_H04
TTTCCGTCTTTCATTTTCCG GGGAGGACGAUGCGGUUUCCGUCUUUCAUUU'UCCGCAGACGACUCGCCCGA 21iG_H04
CTTTATATTG CACTTTTCCG GGGAGGACGAUGCGGCU UUAUAUUGCACUUUUCCGCAGACGACUCGCCCGA 2111_H04
TCTTCATATTTACTCTTCCG GGGAGGACGAUGCGGUCUUCAUAUUUACUCUUCCGCAGACGACUCGCCCGA 2112 H04
# in H4
HIS read variable region RNA sequences 3 selection selection Selected sequenc TTACTTTTAATTTCTTTCCG GGGAGGACGAUGCGGUUACUUUUAAUUUCUUUCCGCAGACGACUCGCCCGA 2 il3_ H04
TCTTTTTTCATTATCTCCCCG GGGAGGACGAUGCGGUCUU UUUUCAUUAUCUCCCCGCAGACGACUCGCCCGA 211 J-TO
TT rci TTGCCTTCCG GGGAGGACGAUGCGGUUU UUUCUUUUUGCCUUCCGCAGACGACUCGCCCGA 2115.J-I04
ATTCTCTTT ITTCCCACCG GGGAGGACGAUGCGGAUUCUCUUUUUUCCCACCGCAGACGACUCGCCCGA 2116_H04
TTTTATTTCAATCTTCTGTG GGGAGGACGAUGCGGUUUUAU UUCAAUCUUCUGUGCAGACGACUCGCCCGA 21i7_ HG4
TTTAcr rACTTTCCCCA GGGAGGACGAUGCGGU UUACUUUUUACUUUCCCCACAGACGACUCGCCCGA 2118_ H04
CTTTCGcrrrTATTT rccG GGGAGGACGAUGCGGCU UUCGCUUUUAUUUUCCGCAGACGACUCGCCCGA 2119 _HQ4
TTTTCTCTAAATTTTTCTCCG GGGAGGACGAUGCGGUUUUCUCUAAAUUUUUCUCCGCAGACGACUCGCCCGA 2120_H04
CTTTACTTTCTTATTTCCCC GGGAGGACGAUGCGGCU iJUACU UUCUUAUUUCCCCCAGACGACUCGCCCGA 2121_H04
TTGTTTATTTTTGTTATCCC GGGAGGACGAUGCGGU UGUUUAUU UUUGUUAUCCCCAGACGACUCGCCCGA 2122JH04
TTCGCTTATATTTTTTTCCG GGGAGGACGAUGCGGU UCGCUUAUAU UUUUU UCCGCAGACGACUCGCCCGA 212.3_Η04
TCTTTTTTTTT G TG'TTT CTC CG GGGAGGACGAUGCGGUCUUUUUUUUUGUGUUU UCCGCAGACGACUCGCCCGA 2124JH04
TTTGGGG f I I I 1 1 CCCGTGCCC GGGAGGACGAUGCGGU UUGGGGUUUUU UCCCGUGCCCCAGACGACUCGCCCGA 2125_H04
TCCTG TTCGTCTGTTCG TCCC GGGAGGACGAUGCGGUCCUGUUCGUCUGUUCGUCCCCAGACGACUCGCCCGA 126._H04
TTTG G G AG TAG G GTG TTCG GGGAGGACGAUGCGGUUUGGGAGUAG6GUG UUCGCAGACGACUCGCCCGA 2127_H04
ATTTAT6 I 1 1 1 ! I CTTGCCG GGGAGGACGAUGCGGAUUUAUGUUU UUUCUUGCCGCAGACGACUCGCCCGA 2.129_H04
AATTCTTATCTTTTTG GCC6 GGGAGGACGAUGCGGAAUUCUUAUCUUUUUGGCCGCAGACGACUCGCCCGA 2130__H04
CTTTTCCTATCCTTTCACCG GGGAGGACGAUGCGGCUUU UCCUAUCCUUUCACCGCAGACGACUCGCCCGA 2131_H04
A TTCG I H l i l 1 1 CTTGTTCCG GGGAGGACGAUGCGGAUUCGUUUUUUUUCUUGUUCCGCAGACGACUCGCCCGA 2132J-I04
ATTACTCTTCTCTTG CCG GGGAGGACGAUGCGGAUUACUCUUCUCUUGCCGCAGACGACUCGCCCGA 2133JH04
TGTTTTTTTTCCCTTTCCCCG GGGAGGACGAUGCGGUG UUUU UUUUCCCUUUCCCCGCAGACGACUCGCCCGA 2135_H04
TTTCTTTGCATTTCTATCCG GGGAGGACGAUGCGGUUUCUUUGCAUUUCUAUCCGCAGACGACUCGCCCGA 2136_H04
ATTTTCTCTTTTTTTTCTGCCG GGGAGGACGAUGCGGAUUUUCUCUUUUUUUUCUGCCGCAGACGACUCGCCCGA 2137_ H04
ATCTCGCTCAATTTTCTCCG GGGAGGACGAUGCGGAUCUCGCUCAAUU UUCUCCGCAGACGACUCGCCCGA 2138_H04
TTGTTTACATCTCCTTTCCG GGGAGGACGAUGCGGUUCUU UACAUCUCCU UUCCGCAGACGACUCGCCCGA 2139 H04
TCTTTCTCTCTTTTGCTCCG GGGAGGACGAUGCGGUCUUUCUCUCUUUUGCUCCGCAGACGACUCGCCCGA 2140_H04
TTCCTATTTCGTTCTTTCCG GGGAGGACGAUGCGGUUCCUAUUUCGUUCUUUCCGCAGACGACUCGCCCGA 2141_H04
TTACTTTCHTTTCCCACCC GGGA.GGACGAUGCGGUUACU UUCUUUUUCCCACCCCAGACGACUCGCCCGA 2143 H04
# in H
HIS read variable region RNA sequences # in H3 selection selection Selected sequenc CTCTTT'CTT'ITATCCTTCCG GGGAGGACGAUGCGGCUCUUUCUUUUAUCCUUCCGCAGACGACUCGCCCGA 2146. HQ4
CATTATTTTTTTTCCCTCG G GGGAGGACGAUGCGGCAUUAU UUUUUU UCCCUCGGCAGACGACUCGCCCGA 2148. „H04
ACTTTTCTTG G TTTTTCCCG GGGAGGACGAUGCGGACUUUUCUUGGUUUUUCGCGCAGACGACUCGCCCGA 2'149_ .H04
TCTTTTTT6TTTTTCTGCCG GGGAGGACGAUGCGGUCUUUU UUG UUUUUCUGCCGCAGACGACUCGGCCGA 2150_ _H04
TTTTTCACTATG CTTTTCTG GGGAGGACGAUGCGGUUUUUCACUAUGCUUUUCUGCAGACGACUCGCCCGA 2151. _H04
TATTTTTTCTT'CTTCTTGTG GGGAGGACGAUGCGGUAU UUUUUCUUCUUCUUG UGCAGACGACUCGCCCGA 2152. _H04
TTCTTGTTCTTATTCATCCG GGGAGGACGAUGCGGUUCU UGUUCUUAUUCAUCCGCAGACGACUCGCCCGA 2153„ H04
TCTTTACTTAAATTTCTCCG GGGAGGACGAUGCGGUCUU UACUUAAAUUUCUCCGCAGACGACUCGCCCGA 2154. .H04
TTCTCTATTTGTTCTTTCCG GGGAGGACGAUGCGGUUCUCUAUUUGUUCUUUCCGCAGACGACUCGCCCGA 2155_ .H04
AATTG CTC ! I I ! 1 I i ! CTACCG GGGAGGACGAUGCGGAAUUGCUCUUUUUUUUCUACCGCAGACGACUCGCCCGA 2156. .H04
ATATTTCACG TTTTTTACTG GGGAGGACGAUGCGGAUAUUUCACGUUUUUUACUGCAGACGACUCGCCCGA 2157. .H04
ATTTTCAGTTTGTTTCACCG GGGAGGACGAUGCGGAUUUUCAGUUUGUUUCACCGCAGACGACUCGCCCGA 2158. „H04
CTTCTCTACTTTTCTTTCCG GGGAGGACGAUGCGGCUUCUCUACU UUUCUU UCCGCAGACGACUCGCCCGA 2159. .H04
ACTTTTTACTTCTTTCACCG GGGAGGACGAUGCGGACUUUUUACUUCUUUCACCGCAGACGACUCGCCCGA 2160. _HG4
TTTCTATTTTATTTTG TGTG GGGAGGACGAUGCGGU UUCUAUUUUAUUUUGUGUGCAGACGACUCGCCCGA 2161. _H04
TTTTACTTTAG CTTTTTCCC GGGAGGACGAUGCGGU UUUACUUUAGCUUU UUCCCCAGACGACUCGCCCGA 2162. _HG4
TTCAl rTTTATTCCCTGTG GGGAGGACGAUGCGGU UCAUUUUUUAUUCCCUG UGCAGACGACUCGCCCGA 2163. _H04
Acm rCTTTCTTATC ccc GGGAGGACGAUGCGGACUUUU UCUUUCU UAUCCCCCAGACGACUCGCCCGA 2164. _H04
TTTTATCTTTTTCATTTCCC GGGAGGACGAUGCGGU UUUAUCUUUUUCAUUUCCCCAGACGACUCGCCCGA 2165. _H04
GTCTTACTATTTCTTTTCCG GGGAGGACGAUGCGGGUCUUACUAUUUCUUUUCCGCAGACGACUCGCCCGA 2166. _H04
TCTT I 1 1 Γ ί ί ί GTTTCCCCCG GGGAGGACGAUGCGGUCUUUUUU UUUGUUUCCCCCGCAGACGACUCGCCCGA 2X67. .H04
TTTTATTCTTTCCTTTACGC GGGAGGACGAUGCGGU UUUAU UCUUUCCUUUACGCCAGACGACUCGCCCGA 2158. JHQ4
TTTTTCTCTTCCTTTGTCCG GGGAGGACGAUGCGGU UUUUCUCUUCCUUUGUCCGCAGACGACUCGCCCGA 2169. _HQ4
ATTCGGTCTTTTTTTTGCTCCG GGGAGGACGAUGCGGAUUCGGUCUUUUUUUUGCUCCGCAGACGACUCGCCCGA 2171. _H04
ATTGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGAUUGGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 2172. _HQ4
ATTATATTCTCTCTCTGCCG GGGAGGACGAUGCGGAUUAUAUUCUCUCUCUGCCGCAGACGAGUCGCCCGA 2173. _H04
ACTTCTTTTTTTGTTTTG CCG GGGAGGACGAUGCGGACUUCUU UUUUUGUUUUGGCGCAGACGACUCGCCCGA 2176. _H04
GTTTATCTCCTTTTTTGCCC GGGAGGACGAUGCGGGUUUAUCUCCUUUUUUGCCCCAGACGACUCGCCCGA 2177 H04
# in H4
HTS read variable region NA sequences selection Selected sequenc TTCTCTG TTCCTTTCTGCCG GGGAGGACGAUGCGGU UCUCUGUUCCUUUCUGCCGCAGACGACUCGCCCGA 2178_H04
GATGGTGGTGTAGGGTACCG GGGAGGACGAUGCGGGAUGGUGGUGUAGGGUACCGCAGACGACUCGCCCGA 2180_ H04
TTTCTTACTTCCTCGTTCCG GGGAGGACGAUGCGGUUUCUUACUUCCUCGUUCCGCAGACGACUCGCCCGA 2182JHQ4
TTTTTCTCTCTACATTTCCG GGGAGGACGAUGCGGUUUUUCUCUCUACAUUUCCGCAGACGACUCGCCCGA 21S3, H04
TTATTTTCCGCCTTTTGCCG GGGAGGACGAUGCGGUUAU UUUCCGCCUUUUGCCGCAGACGACUCGCCCGA 2184_HG
TTCTCTATP] rCTTGCCG GGGAGGACGAUGCGGUUCUCUAUUUUUUCUUGCCeCAGACGACUCGCCCGA 2185_H04
AC ATTATCCTG CATGTACCG GGGAGGACGAUGCGGACAUUAUCCUGCAUG UACCGCAGACGACUCGCCCGA 2186_H04
ATCTTTCTTTCTTTCTGTCCG G6GAGGACGAUGCGGAUCUU UCUUUCUUUCUGUCCGCAGACGACUCGCCCGA 218?„H04
TTTTCTATTTGTTG CTTCCG GGGAGGACGAUGCGGUUUUCUAU UUGUUGCUUCCGCAGACGACUCGCCCGA 2189__HG4
TTl'ACATTTTCTCCTCTCCG GGGAGGACGAUGCGGU UUACAUUU UCUCCUCUCCGCAGACGACUCGCCCGA 21S0_H04
TATTCTTATGTTACTTTCCG GGGAGGACGAUGCGGUAUUCUUAUG UUACUU UCCGCAGACGACUCGCCCGA 2191_H04
CATTCTTTTTTCTCCTGTG GGGAGGACGAUGCGGCAUUCUUUU UUCUCCUGUGCAGACGACUCGCCCGA 2192_H04
CTTTATTTFCGGTTTTACCG GGGAGGACGAUGCGGCU UUAU UUUCGGU UU UACCGCAGACGACUCGCCCGA 2193„H04
CTCTTTTTTCTCCATATCCG GGGAGGACGAUGCGGCUCUUUU UUCUCCAUAUCCGCAGACGACUCGCCCGA 219 JH04
CTCTCTCTTTGTTTTCTCCG GGGAGGACGAUGCGGCUCUCUCUUUGUUUUCUCCGCAGACGACUCGCCCGA 2195_H04
ATTCTTGTTTTTTTTGCCG GGGAGGACGAUGCGGAUUCUUGUUUUUUUUGCCGCAGACGACUCGCCCGA 2196_H04
ATTCGTTTTTTCTTGTTCCG GGGAGGACGAUGCGGAUUCGUUUU UUCU UGULCCGCAGACGACUCGCCCGA 2197_H04
ATT7TCCGTTTCTTACTCCG GGGAGGACGAUGCGGAUUUUCCCUU UCUUACUCCGCAGACGACUCGCCCGA 2198_H04
CTTTATTTTTTG'fTTTTTGCCC GGGAGGACGAUGCGGCUUUAUUUU UUGUUU UUUGCCCCAGACGACUCGCCCGA 2199„H04
TTTGGGAGTAGGGTGTTCCA GGGAGGACGAUGCGGUUUGGGAGUAGGGUGUUCCACAGACGACUCGCCCGA 2200_H04
TTmCCTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUUUCCUUUCCUU UGUCCGCAGACGACUCGCCCGA 2201_H04
ACCTTTAATTACTC CTTCCG GGGAGGACGAUGCGGACCUU UAAUUACUCCU UCCGCAGACGACUCGCCCGA 2202_H04
TTTCCACG GTTTTTTTCCG GGGAGGACGAUGCGGUUUCCACGGUUUUUUUCCGCAGACGACUCGCCCGA 2203_H04
TTCTTTATAArrATTCTCCG GG6AGGACGAUGCGGUUCUUUAUAAUUAUUCUCCGCAGACGACUCGCCCGA 2204_H04
GTTCTTAATTCTTTTCACCG GGGAGGACGAUGCGGGUUCU UAAUUCUUUUCACCGCAGACGACUCGCCCGA 2205JH04
rrrriCATTcccTTTTTTCCG GGGAGGACGAUGCGGUUUU UCAUUCCCUUUUUUCCGCAGACGACUCGCCCGA 22Q6_H0
TTTTTCTATTACTTTCTG CC GGGAGCACGAUGCGGUUUU UCUAUUACUUUCUGCCCAGACGACUCGCCCGA 2209_H04
TCCTTTATTTCTCTTCG CCG GGGAGGACGAUGCGGUCCUU UAUUUCUCUUCGCCGCAGACGACUCGCCCGA 2210 H04
U in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CACTTATCTTATTCTCTCCe GGGAGGACGAUGCGGCACUUAUCUUAUUCUCUCCGCAGACGACUCGCCCGA 2211. _H04
TTTCCGTTCTTCTTCATCCG GGGAGGACGAUGCGGU UUCCGUUCUUCUUCAUCCGCAGACGACUCGCCCGA 2212. _H04
TTTTCTCG CTTCTATGTCCG GGGAGGACGAUGCGGU UUUCUCGCUUCUAUG UCCGCAGACGACUCGCCCGA 2213_ _H04
TTTTTATGGTTTGTTTCCG GGGAGGACGAUGCGGUUUUUAUGGUUUGUUUCCGCAGACGACUCGCCCGA 2214. .H04
ATCTTTCTCTTCTTGTCCCG GGGAGGACGAUGCGGAUCU UUCUCUUCUUGUCCCGCAGACGACUCGCCCGA 2216. JHG4
TTTTTC6CTGTCTTTTTCCG" GGGAGGACGAUGCGGUUU UUCGCUGUCU UUULCCGCAGACGACUCGCCCGA 2217. .H04
TGTTTATTCTTGTTTGTCCG GGGAGGACGAUGCGGUGUUUAUUCUUGUUUGUCCGCAGACGACUCGCCCGA 2218. .H04
TCTCTTCTTTATTCT CCG GGGAGGACGAUGCGGUCUCUUCUUUAU UCUUCCGCAGACGACUCGCCCGA 2219. _H04
AA7TTCATTCTCTTTTTG CC GGGAGGACGAUGCGGAAUUUCAUUCUCUUUUUGCCCAGACGACUCGCCCGA 2220. Q4
CTTTTATCACATTCTTTCCG GGGAGGACGAUGCGGCU UUUAUCACAUUCUUUCCGCAGACGACUCGCCCGA 2222_ _H04
GCATTTCTTTTCCCTTACCG GGGAGGACGAUGCGGGCAUUUCUUUUCCCUUACCGCAGACGACUCGCCCGA 2223.. JH04
TCCATGTTGTTTTTTTTCCG GGGAGGACGAUGCGGUCCAUGUUGUUUUUUUUCCGCAGACGACUCGCCCGA 2224. _H04
CATTTTTCATTCCTTTTCCG GGGAGGACGAUGCGGCAUUUUUCAUUCCUUUUCCGCAGACGACUCGCCCGA 2225. _H04
ATTTTCTTTG CTTTATTCTG GGGAGGACGAUGCGGAUUUUCUUUGCUU UAUUCUGCAGACGACUCGCCCGA 2226. _H04
CTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGCUUUCGUUCUGCCCUCCCCAGAGGACUCGCCGGA 2227. .H04
ACTTTTTTTCCTCTTACCCG GGGAGGACGAUGCGGACUUUUUUUCCUCU UACCCGCAGACGACUCGCCCGA 2228. _H04
AAC CTTG TTTTCTTTC ACCG GGGAGGACGAUGCGGAACCUUGUUU UCUUUCACCGCAGACGACUCGCCCGA 2229. .H04
GTTTTTCTTTACTCTTTCCC GGGAGGACGAUGCGGGUUUUUCUUUACUCUUUCCCCAGACGACUCGCCCGA 2230. _HQ4
ATTTTTTTCTTTCCTTTG CCG GGGAGGACGAUGCGGAUUU UUUUCUUUCCUUUGCCGCAGACGACUCGCCCGA 2231. _H04
TTTGTAATGTTTTTTTTTCC6 GGGAGGACGAUGCGGUUUG UAAUGUUUUUU UUUCCGCAGACGACUCGCCCGA 2233. .H04
TTTTTTCATTCCCTTTTTCCG GGGAGGACGAUGCGGUUUUUUGAUUCCCUUUUUCCGCAGACGACUCGCCCGA 2234.. .H04
CTTTCTATAATTATTCTCCG GGGAGGACGAUGCGGCUUUCUAUAAUUAU UCUCCGCAGACGACUCGCCCGA 2235. _H04
TTTATTGTTTATTTGTTCCA GGGAGGACGAUGCGGUUUAUUGUUUAUUUGUUCCACAGACGACUCGCCCGA 2237. _H04
TGTTTTTTGG I I I I I I TGCG GGGAGGACGAUGCGGUG UUUU UUGGUUUUUUUGCGGAGACGACUCGCCCGA 2238. .H04
TTCGTAC I I ! 1 1 I i U !TTCCG GGGAGGACGAUGCGGUUCGUACUUU UUUUUU UUUCCGCAGACGACUCGCCCGA 2239. _H04
rG G 77 ATATTTCTCCG GGGAGGACGAUGCGGU UUUUGGUUAUAUUUCUCCGCAGACGACUCGCCCGA 2240. _H04
TTTTATTTCCATTTTTGCAC GGGAGGACGAUGCGGUUUUAUUUCCAUUUUUGCACCAGACGACUCGCCCGA 2241. _HQ4
"(TCATTTTAGTTTTTTCCCC GGGAGGACGAUGCGGUUCAUUU UAGUU UUUUCCCCCAGACGACUCGCCCGA 2242 H04
in m
HTS read variable region RNA sequences selection selection Selected sequenc
CC7TACTATACTTTTTTCCG GGGAGGACGAUGCGGCCUUACUAUACUUUUUUCCGCAGACGACUCGCCCGA. 2243_H04
TTAAGGAGAAGGGTGGTACG GGGAGGACGAUGCGGUUAAGGAGAAGGGUGGUACGCAGACGACUCGCCCGA 2244 H04
TTTTTCATACCTTTTTTTCCG GGGAGGACGAUGCGGU UUUUCAUACCUUUUUUUCCGCAGACGACUCGCCCGA 2245„H04
TTTTTGTTGGTGTTT'iTFCCG GGGAGGACGAUGC6GU UUUUGUUGGUGUUUUUUCCGCAGAC6ACUCGCCCGA 2246_ H04
TATTTTTTCCTTGTTTTCTG GGGAGGACGAUGCGGUAUU UUUUCCUUG UUUUCUGCAGACGACUCGCCCGA 2247_H04
TTTTTCATTTATTACTTCTG GGGAGGACGAUGCGGUUUU UCAUUUAUUACUUCUGCAGACGACUCGCCCGA 2248_H04
TCCTGTCGTCTTTGTCCC 6GGAGGACGAUGCGGUCCUGUCGUCUUU6UCCCCAGACGACUCGCCCGA 2250_H04
ΑΑΤΤΑΤ6ΤΊ "ΓΠ ΊΊ GTGACCG GGGAGGACGAUGCGGAAUUAUGUUUUUUUGUGACCGCAGACGACUCGCCCGA 2251„H04
TCCTGTCGTCTCTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUCUGUCCCCAGACGACUCGCCCGA 2252JH04
TGCTTTCTCATTTCTTTCCG GGGAGGACGAUGCGGUGCUUUCUCAUUUCUUUCCGCAGACGACUCGCCCGA 2255_H04
AATTATGTG M ! 1 1 GACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUUUUGACCGCAGACGACUCGCCCGA 225?_H04
CATAGGAGTAGGGTGGTGC GGGAGGACGAUGCGGCAUAGGAG UAGGGUGGUGCCAGACGACUCGCCCGA 2258_H04
TTTCTTCTACTATCTATCCG GGGAGGACGAUGCGGUUUCUUCUACUAUCUAUCCGCAGACGACUCGCCCGA 2259_H04
ATCCATTATACTTTTCTCCG GGGAGGACGAUGCGGAUCCAU UAUACUUUUCUCCGCAGACGACUCGCCCGA 2260_H04
TTTGGGAGTAGAGTGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAGAGUGUUCCGCAGACGACUCGCCCGA 2261.. H04
GTTTTCCTTTTCCCTTTGCC GCGAGGACGAUGCGGG UUUUCCUUUUCCCUUUGCCCAGACGACUCGCCCGA 2262_H04
TCATTTTATTTTTTCTTC CG GGGAGGACGAUGCGGUCAUUUUAUUUUUUCUUCCGCAGACGACUCGCCCGA 2265_HG4
TAGTTTTCCTTTCTTTTCCG GGGAGGACGAUGCGGUAGUUUUCCU UUCUUUUCCGCAGACGACUCGCCCGA 2266_H04
AGATTTGTTTTTTGTGGCCG GGGAGGACGAUGCGGAGAUUUGUU UUUUGUGGCCGCAGACGACUCGCCCGA 2267_HQ4
TCCTTTAATTTTCTTTG CCG 6GGAGGACGAUGC6GUCCUUUAAUUUUCUUUGCCGCAGACGACUCGCCCGA 2268JH04
ATTGGTGTAGGGGGGTTCCG GGGAGGACGAUGCGGAUUGG UG UAGGGGGG UUCCGCAGACGACUC6CCCGA 22-69_H04
TTATCTTGTTCTTTCATCCC GGGAGGACGAUGCGGUUAUCUUGUUCUUUCAUCCCCAGACGACUCGCCCGA 2272_H04
TTTTTCTCATTTTCTTAC CG GGGAGGACGAUGCGGUUUUUCUCAUUUUCUUACCGCAGACGACUCGCCCGA 2273JH04
ATTCTTTTC S M M I CGCTG GGGAGGACGAUGCGGAU UCU UUUCUUUUUUCGCUGCAGACGACUCGCCCGA 2274_H04
ATTTTTTCATTCCTTTTCCG GGGAGGACGAUGCGGAU UUUUUCAU UCCUUUUCCGCAGACGACUCGCCCGA 2275_H04
AGTTTCATGTTTTTTTGTCCC GGGAGGACGAUGCGGAG UUUCAUGUUUUUUUGUCCCCAGACGACUCGCCCGA 2276_H04
ACTTTTCATTTC ATTTG CCG GGGAGGACGAUGCGGACU UUUCAUUUCAUUUGCCGCAGACGACUCGCCCGA 2277mH04
ATTTCAATGTTCTTTCTCCG GGGAGGACGAUGCGGAUUUCAAUGUUCUUUCUCCGCAGACGACUCGCCCGA 2278 H04
U in H4
HTS read variable region RNA sequences election selection Selected sequenc TCTTGTTTTTTTTTG GCCC GGGAGGACGAUGCGGUCUUGUUUUUUUUUGGCCCCAGACGACUCGCCCGA 2279_HQ4
TTTCTCTTATCTTCTG CCG GGGAGGACGAUGCGGUUUCUCUUAUCUUCUGCCGCAGACGACUCGCCCGA 2280 H04
TTTTCCTTCTTTTCCTTCCG GGGAGGACGAUGCGGUUUUCCUUCUUUUCCUUCCGCAGACGACUCGCCCGA 2281JH04
TTTCACACTTTTTTTG CTG GGGAGGACGAUGCGGUUUCACACUUUUUUUGCUGCAGACGACUCGCCCGA 2283_H04
TTCCTTA ATTTCTTTC CCCG GGGAGGACGAUGCGGUUCCUUAAUUUCUU UCCCCGCAGACGACUCGCCCGA 2284. H04
AGTTTTGACTCTCTTTTCCG GGGAGGACGAUGCGGAG UUUUGACUCUCUUUUCCGCAGACGACUCGCCCGA 2285_H04
CTTGTGTTTTTTTTTTGCC GGGAGGACGAUGCGGCUUGUGUUUUUUUUUUGCCCAGACGACUCGCCCGA 2286_H04
TTTATATGTTTCTTTCCCCG GGGAGGACGAUGCGGU ULAUAUGUUUCU UUCCCCGCAGACGACUCGCCCGA 2287J-I04
CAmTTCcrrcrrcrrcGc GGGAGGACGAUGCGGCAUUUUUCCUUCUUCUUCGCCAGACGACUCGCCCGA 2288„H04
TTTTTCTATG TTTATTTCTG GGGAGGACGAUGCGGU UUU UCUAUGUUUAUUUCUGCAGACGACUCGCCCGA 2289_H04
GTATTTTTGTTCTTCTCCCG GGGAGGACGAUGCGGGUAUUUUUGUUCUUCUCCCGCAGACGACUCGCCCGA 2290_H04
TTTATCTTTAATTTCCCCCG GGGAGGACGAUGCGGUUUAUCUUUAAUU UCCCCCGCAGACGACUCGCCCGA 2291_H04
ATTCTTTATCTCTTTTTGCC GGGAGGACGAUGCGGAUUCUUUAUCUCUU UUUGCCCAGACGACUCGCCCGA 2292_H04
TTTTCTTATTTTCACATCCC GGGAGGACGAUGCGGUUUUCUUAUUUUCACAUCCCCAGACGACUCGCCCGA 2293__H04
τατατΑτπττΰπΑτςςο GGGAGGACGAUGCGGUCUUGUAUUUUUG UUAUCCGCAGACGACUCGCCCGA 2294_ H04
TTTTTACTATTTTACTTCTG GGGAGGACGAUGCGGUUUUUAC'JAUUUUACUUCUGCAGACGACUCGCCCGA 2295_H04
TTTCTTTACTTTTACCTCTG GGGAGGACGAUGCGGUUUCUUUACUU UUACCUCUGCAGACGACUCGCCCGA 2296_H04
TGTTTTTGTTTATTTTTGCCG GGGAGGACGAUGCGGUG UUUU UGUUUAUUUUUGCCGCAGACGACUCGCCCGA 2297_H04
TCTTTTTTCACTTTCTCCG GGGAGGACGAUGCGGUCUUUUUUCACUUUCUCCGCAGACGACUCGCCCGA 229S_H04
ATCACTATTTTTTTTCACCG GGeAGGACGAUGCGGAUCACUAUUUUUUUUCACCGCAGACGACUCGCCCGA 2299_H04
CATATTTCTTC 1 I t I i I CCG GGGAGGACGAUGCGGCAUAUUUCUUCUUUUUUCCGCAGACGACUCGCCCGA 23OOJH04
CTCTTTCTTTTCATCTTCCG GGGAGGACGAUGCGGCUCUUUCUUUUCAUCUUCCGCAGACGACUCGCCCGA 230l_H04
TTATTTTATTCTTCTCTCTG GGGAGGACGAUGCGGUUAUUUUAUUCUUCUCUCUGCAGACGACUCGCCCGA 2302_H04
ATCTCTTTCACTTATTACCG GGGAGGACGAUGCGGAUCUCUUUCACUUAUUACCGCAGACGACUCGCCCGA 2303_H04
TTTTTTTAGTTATTCTCTCCG GGGAGGACGAUGCGGUUUU UUUAGUUAUUCUCUCCGCAGACGACUCGCCCGA 2304_HQ4
TCTTTTTTTGTTTTTCCG CCG GGGAGGACGAUGCGGUCUUU UUUUGUUUUUCCGCCGCAGACGACUCGCCCGA 2305_H04
TTTCCA CG GTTTTTTTTTTCCG GGGAGGACGAUGCGGUU UCCACGGUU U UUUU UUUCCGCAGACGACUCGCCCGA 2306_H04
TTATTTTTTTTGTTTGTTGTG GGGAGGACGAUGCGGUUAUUUU UUUUGUUUGU UGUGCAGACGACUCGCCCGA 23Q7 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TCCTTGTCGTCTTG CG TTCC GGGAGGACGAUGCGGUCCUUGUCGUCUUGCGUUCCCAGACGACUCGCCCGA 2308_H04
AAAGGAG G AG TTG G GATCCG GGGAGGACGAUGCGGAAAGGAGGAG UUGGGAUCCGCAGACGACUC6CCCGA 2309_H04
AATTATGTGTTTGTGACCG GGGAGGACGAUGCGGAAUUAUGUGUU UGUGACCGCAGACGACUCGCCCGA 231Q_H04
TTTGGGAGTAGG6TGGTCCG GGGA6GACGAUGCGGUUUGGGAG UAGGGUGGUCCGCAGACGACUCGCCCGA 2311_H04
TTTCTACTATGCCCTTTCCG GGGAGGACGAUGCGGUULiCUACUAUGCCCUUUCCGCAGACGACUCGCCCGA 2312_H04
CGTTATTTCTCTTTTTGCTG GGGAGGACGAU6CGGCGUUAUUUCUCUUUUUGCUGCAGACGACUCGCCCGA 2314_H04
TCACGTTCTCCTAG TCCCCC GGGAGGACGAUGCGGUCACGUUCUCCUAGUCCCCCCAGACGACUCGCCCGA 2315_HQ4
GTTTCCTCGTTTTCTCTCCG GGGAGGACGAUGCGGGUUUCCUCGUUUUCUCUCCGCAGACGACUCGCCCGA 2316_H04
TACTTTCCTTATTTG CTCTCCG GGGAGGACGAUGCGGUACUUUCCUUAUUUGCUCUCCGCAGACGACUCGCCCGA 2317_H04
CGTTCATTTTCTTCTTACCG GGGAGGACGAUGCGGCGUUCAU UUUCUUCUUACCGCAGACGACUCGCCCGA 2318_H04
CTTTTCCTCTTTCTTCTGCC GGGAGGACGAUGCGGCUUUUCCUCUUUCUUCUGCCCAGACGACUCGCCCGA 2319_H04
TCTTTTC.GTACTTG CTTCCG GGGAGGACGAUGCGGUCUUUUCGUACUUGCUUCCGCAGACGACUCGCCCGA 2320_H04
CTTTCTTGACCCTTTCTCCG GGGAGGACGAUGCGGCU UUCUUGACCCUUUCUCCGCAGACGACUCGCCCGA 2321_H04
TTTGGGAGTAGTGTGTTCCG GGGAGGACGAUGCGGUUUGGGAGUAGUGUGUUCCGCAGACGACUCGCCCGA 2322_H04
AGTTTCATGTTTTTTGTCCC GGGAGGACGAUGCGGAGUUUCAUGUUUUUUG UCCCCAGACGACUCGCCCGA 2323_H04
ATTCGTTTTCATTATTACCG GGGAGGACGAUGCGGAUUCG UU UUCAUUAUUACCGCAGACGACUCGCCCGA 2324_^HG4
TCACTTTTCCTTTATCCCCG GGGAGGACGAUGCGGUCACUUUUCCUUUAUCCCCGCAGACGACUCGCCCGA 2325„H04
ATTCCAC I I 1 1 1 ! N I CACCG GGGAGGACGAUGCGGAUUCCACUUUUUUUUUCACCGCAGACGACUCGCCCGA 2326_ HQ4
CTTTTTTGTATTTGTTTCCG G6GAGGACGAUGCGGCUUUUUUGUAUUUGUUUCCGCAGACGACUC6CCCGA 2 27_H04
TTTGGTTTTTTTTTGCCCG GGGAGGACGAUGCGGUUUGGUUUUUUUUUGCCCGCAGACGACUCGCCCGA 2328_H04
TCTrmArrrrccTCGGCc GGGAGGACGAUGCGGUCUUUUUAUUUUCCUCGGCCCAGACGACUCGCCCGA 2329_H04
TGTTTTG I Ϊ ' Π ITGTCCCC GGGAGGACGAUGCGGUGUUUUGUUUUUUUG UCCCCCAGACGACUCGCCCGA 2330_H04
TTTTTTTCCTCG CTTTTG CCG GGGAGGACGAUGCGG UUUUUUUCCUCGCUUUUGCCGCAGACGACUCGCCCGA 2331„H04
G ΤΓ ΑΤΊΤ ACGTTTTTTTC CG GGGAGGACGAUGCGGGUUAU UUACCUUUUUU UCCGCAGACGACUCGCCCGA 233.3_.HC4
CTCTTTAAAATTCTCTTCCG GGGAGGACGAUGC6GCUCUU UAAAAUUCUCUUCCGCAGACGACLICGCCCGA 2334_H04
TATTTTCTTTTTCTTCGCAC GGGAGGACGAUGCGGUAUUUUCUUUUUCUUCGCACCAGACGACUCGCCCGA 233S_H04
TTTATTTCTTATTGTTTGCG GGGAGGACGAUGCGGUUUAUUUCUUAUUGUUUGCGCAGACGACUCGCCCGA 2336_H04
GTTTTTACTTTTTTACTCCC GGGAGGACGAUGCGGGU UUUUACUU UUUUACUCCCCAGACGACUCGCCCGA 2337 H04
# in H4
HTS read variable region R A sequences # in H3 se selection Selected sequenc
CTT CT ATATTTTCTACTCCG GGGAGGACGAUGCGGCU UCUAUAUUUUCUACUCCGCAGACGACUCGCCCGA 2338_H04
TTTCTTTACTTACTCTCCCG GGGAGGACGAUGCGGUUUCUUUACUUACUCUCCCGCAGACGACUCGCCCGA 2339_H04
GTTTATG TTTACCTTTCGTC GGGAGGACGAUGCGGGUUUAUGUUUACCUUUCGUCCAGACGACUCGCCCGA 2340„H04
ATATTTTATCTCTI'TTCCCG GGGAGGACGAUGCGGAUAUUUUAUCUCUUUUCCCGCAGACGACUCGCCCGA 2341JH04
ACACTATTTTCCTTTTTCCC GGGAGGACGAUGCGGACACUAUUUUCCUUUUUCCCCAGACGACUCGCCCGA 2342_H04
ATCTTCTTGACTCTTTACCG GGGAGGACGAUGCGGAUCUUCU UGACUCU UUACCGCAGACGACUCGCCCGA 2343_H04
CCTTTTTTCTATCCTTTCCG GGGAGGACGAUGCGGCCUUU UUUCUAUCCUUUCCGCAGACGACUCGCCCGA 2344_H04
TCTTCAGGTTTATTTTTCCG GGGAGGACGAUGCGGUCUUCAGGUUUAUUUUUeCGCAGACGACUCGCCCGA 2345_H04
TTTCTATTAATCC TTTCTG GGGAGGACGAUGCGGUU UCUAU UAAUCCUUUUCUGCAGACGACUCGCCCGA 2346_HQ4
TACTTTTC I l i l t I'CTCCG GGGAGGACGAUGCGGUACUUUUCUUUUUUCUCCGCAGACGACUCGCCCGA 2347 JHQ4
TCTTGTCTTAATTTTTCCCG GGGAGGACGAUGCGGUCUUGUCUUAAUUUUUCCCGCAGACGACUCGCCCGA 2348_H04
GTTTTTCTTTTTCCTTACCG GGGAGGACGAUGCGGGUUU UUCUUUUUCCUUACCGCAGACGACUCGCCCGA 2349„H04
GTTTGTTCTTTTTTTTCCG GGGAGGAC6AUGCGGGUUU6UUCUUUUUUU UCCGCAGACGACUC6CCCGA 23SCLH04
TTTTGTTCTGTTATTTTCCC GGGAGGACGAUGCGGUUUUGUUCUGUUAUU UUCCCCAGACGACUCGCCCGA 2351_H04
TGTTTTATTTATCCTTTCG C GGGAGGACGAUGCGGUGUUUUAUUUAUCCUUUCGCCAGACGACUCGCCCGA 2352„H04
GTTGTTTTAATGTTTTTCCC GGGAGGACGAUGCGGGUUGUUUUAAUGUUUUUCCCCAGACGACUCGCCCGA 2353_H04
GATTCTCTTATTTCTTACCC GGGAGGACGAUGCGGGAUUCUCUUAUUUCUUACCCCAGACGACUCGCCCGA 2356„H04
TTTTACTTTTTTTTTGTG GGGAGGACGAUGCGGU U UUACUUUUUUU UUGUGCAGACGACUCGCCCGA 23S7_H04
TTTTATTTCTTCTTTGT6TC GGGAGGACGAUGCGGUU IJUAUUUCUUCUUUG UGUCCAGACGACUCGCCCGA 2358_H04
TTTATTCTGTTTTTTTTTTCCG GGGAGGACGAUGCGGUU UAUUCUGUUUU UUUUU UCCGCAGACGACUCGCCCGA 2359_H04
CTTTCTAATCTATTTTTCCG GGGAGGACGAUGCGGCU UUCUAAUCUAUUUUUCCGCAGAC6ACUCGCCCGA 2360_H04
TTTGGGGTTTICCCGTCCCC GGGAGGACGAUGCGGUUUGGGGUUUUCCCGUCCCCCAGACGACUCGCCCGA 2361„H04
TCCTGTCGTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCG UGUUCGUCCCCAGACGACUCGCCCGA 2362_H04
TCCTTGTCGTCTTGCGCCCC GGGAGGACGAUGCGGUCCUUGUCGUCUUGCGCCCCCAGACGACUCGCCCGA 2363_H04
GTTGGTGTAGGGAGGTTCCG GGGAGGACGAUGCGGGU UGGUGUAGGGAGGUUCCGCAGACGACUCGCCCGA 2365_hi04
ATTTGTCTTTTGTGTTACCG GGGAGGACGAUGCGGAUUUGUCUUUUGUGUUACC6CAGACGACUCGCCCGA 2366_H04
T CrrCTTTTCTTCTGCTCCG GGGAGGACGAUGCGGUCUUCUUUUCUUCUGCUCCGCAGACGACUCGCCCGA 2367_H04
ATTTGTTCATGTTTCCTCCG GGGAGGACGAUGCGGAU UUG UUCAUGUUUCCUCCGCAGACGACUCGCCCGA 2368_H04
# irs H4
HTS read variable region RNA sequences selection Selected sequenc
CACTACTTTATTCCTCTCCG GGGAGGACGAUGCGGCACUACUUUAUUCCUCUCCGCAGACGACUCGCCCGA 2365_H04
GTTTTCAATCGCTCTTTCC6 GGGAGGACGAUGCGGGUUUUCAAUCGCUCUUUCCGCAGACGACUCGCCCGA 237D_H04
TACTTTCTCATTTTCTGCTG GGGAGGACGAUGCGGUACUUUCUCAUUUUCUGCUGCAGACGACUCGCCCGA 2371_H04
ATTTATTATCGCTGTTTCCG GGGAGGACGAUGCGGAUUUAUUAUCGCUGUUUCCGCAGACGACUCGCCCGA 2374_H04
CTTTTTACG C C CTTTTTCCG GGGAGGACGAUGCGGCUUUUUACGCCCUUUUUCCGCAGACGACUCGCCCGA 2375_H04
TTTCC AGAGTTTTTTGTTCCG GGGAGGACGAU6C6GU UUCCUAGAGUUUUUUGU UCCGCAGACGACUCGCCCGA 2376_H04
GTTTTTATTCTCTCATTCCC GGGAGGACGAUGGGGGUUUUUAUUCUCUCAUUCCCCAGACGACUCGCCCGA 2377_H04
TTTCATACTTCCTACTTCCG GGGAGGACGAUGCGGU UUCAUACUUCCUACUUCCGCAGACGACUCGCCCGA 2379_H04
CTTTTCCTTTTTTTTTGTTCCG GGGAGGACGAUGCGGCUUUUCCUU UUUUUU UGUUCCGCAGACGACUCGCCCGA 2380_H04
ACTTTG CTCTTGTTTTACCC GGGAGGACGAUGCGGACUU UGCUCUUGUUUUACCCCAGACGACUCGCCCGA 2381_H04
TC7TTATTTA7TG CTATCCG GGGAGGACGAUGCGGUCUUUAUUUAUUGCUAUCCGCAGACGACUCGCCCGA 2382_H04
CTTTCGCTTTTTATCTTCCG GGGAGGACGAUGCGGCUUUCGCUUUUUAUCU UCCGCAGACGACUCGCCCGA 2383„H04
TTTTCACATTCTTTTCTCGC GGGAGGACGAUGCGGUUUUCACAUUCUUUUCUCGCCAGACGACUCGCCCGA 2384_.H04
ATCTTAATCTCTTTTCrCCG GGGAGGACGAUGCGGAUCU UAAUCUCUUUUCUCCGCAGACGACUCGCCCGA 2385_H04
TCTTTAATTATTCCCCTCCG GGGAGGACGAUGCGGUCUUUAAUUAUUCCCCUCCGCAGACGACUCGCCCGA 238S_ H04
AATTCCTTTTATCTTTGCGC GGGAGGACGAUGCGGAAUUCCU UUUAUCUUUGCCCCAGACGACUCGCCCGA 2387_H04
GTTTCTTATTATCTTTCCCC GGGAGGACGAUGCGGGUUUCUUAUUAUCUUUCCCCCAGACGACUCGCCCGA 2388_HQ4
TATCTCTGGCCTTTTTTCCG GGGAGGACGAUGCGGUAUCUCUGGCCUUUUUUCCGCAGACGACUCGCCCGA 2389 _H04
TTATTTTTGTTTGTTTGCCC GGGAGGACGAUGCGGUUAUUUUUGUUUGUUUGCCCCAGACGACUCGCCCGA 2391_H04
ACTTTTTATCTTTCCAACCG GGGAGGACGAUGCGGACUU UUUAUCUUUCCAACCGCAGACGACUCGCCCGA 2393_ H04
ATTCTTTACACTATTCACCG GGGAGGACGAUGCGGAUUCUUUACACUAUUCACCGCAGACGACUCGCCCGA 2394_H04
CTTTTTCAATCCTTCTTCCG GGGAGGACGAUGCGGCUUUUUCAAUCCUUCUUCCGCAGACGACUCGCCCGA 2395_H04
AC T Ί" Π "I f C CTTTCTTCC G GGGAGGACGAUGCGGACUUU UUUCACUUUCU UCCGCAGACGACUCGCCCGA 2397_H04
GATTGTTTATTTTTTACCCC GGGAGGACGAUGCGGGAUUGU UUAUUUUUUACCCCCAGACGACUCGCCC6A 2398 _H04
CTTCTGTTTTCATTCTTCCG GGGAGGACGAUGCGGCUUCUGUUU UCAUUCUUCCGCAGACGACUCGCCCGA 2399 _H04
TTTCA.I I N N ! ATTCTCCG GGGAGGACGAUGCGGU UUCAUUU UUUUAUUCUCCGCAGACGACUCGCCCGA 2400_H04
TCATTCTTTTTATTCCTCTG GGGAGGACGAUGCGGUCAUUCUUUUUAUUCCUCUGCAGACGACUCGCCCGA 2401. H04
TTTCTGACACTTTTTTTTCCG GGGAGGACGAUGCGGUUUCUGACACUUUUUUUUCCGCAGACGACUCGCCCGA 2403 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
ACTTTCTATATTTCATTCCG GGGAGGACGAUGCGGACUUUCUAUAUU UCAUUCCGCAGACGACUCGCCCGA 2404. _H04
ATTTATTTATGCTTTTTCCC GGGAGGACGAUGCGGAUUUAUUUAUGCUUU UUCCCCA.GACGACUCGCCCGA 2 05. „H04
TTTG TTTTTTTTGTTTCTG GGGAGGACGAUGCGGUUUGUU UU UUUUGUUUCUGCAGACGACUCGCCCGA 2406. .H04
TATTCTCTTTTTCCTTTCTG GGGA6GACGAUGCGGUAUUCUCUUUUUCCUUUCUGCAGACGACUCGCCCGA 2407. .H04
ATATTTTCCATCTTTTGCTG GGGAGGACGAUGCGGAUAUUUUCCAUCUUUUGCUGCAGACGACUCGCCCGA 2409. H04
TCTTTTATTTTATTTCCCCC GGGAGGACGAUGCGGUCUUUUAUUUUAU UUCCCCCCAGACGACUCGCCCGA 2410_ H04
CTTTTATTCTATTTTTTCC6 SGGAGGACGAUGCGGCUUUUAUUCUAUUUUUUCCGCAGACGACUCGCCCGA 2411. H04
TTTTTATTTTTCTTTrCG CCA GGGAGGACGAUGCGGUUUU UAUUUUUCUUUUCGCCACAGACGACUCGCCCGA 2412_ H04
CTTTA ! l I I i rCCTCTTGCC GGGAGGACGAUGCGGCUUUAUUUUUUCCUCUUGCCCAGACGACUCGCCCGA 2413. H04
CTTCTTTATTTCTCTCTCGC GGGAGGACGAUGCGGCUUCU UUAUUUCUCUCUCGCCAGACGACUCGCCCGA 2415. .H04
ATTGTITTATTGTTTTTACCG GGGAGGACGAUGCGGAUUGUUUUAUUGUUUUUACCGCAGACGACUCGCCCGA 2416. H04
ATAnATTTTGTTTrTTTGCC GGGAGGACGAUGCGGAUAUUAUUUUGUUUUUUUGCCCAGACGACUCGCCCGA 2417. _H0
ATTTACTTTTTATTACTGTG GGGAGGACGAUGCGGAUUUACU UUUUAUUACUGIJGCAGACGACUCGCCCGA 2418. Q4
TTTTCCTTATACTTTTGCG GGGAGGACGAUGCGGUU UUCCUUAUACUUUUCCGCAGACGACUCGCCCGA 2419. H04
CCATTTCTTCATTTTTTTCCG GGGAGGACGAUGCGGCCAUUUCUUCAU UUUUU UCCGCAGACGACUCGCCCGA 2420. _H04
TCCT6TCGGCTGTGCGTCCC GGGAGGACGAUGCGGUCCU6UCGGCUGUGCGUCCCCAGACGACUCGCCCGA 2421. _H04
TCCTTTCGTTCTGCCTTCCC GGGAGGACGAUGCGGUCCUUUCG UUCUGCCUUCCCCAGACGACUCGCCCGA 2423. _H04
TTCTCATTTCTTCACCTCCG GGGAGGACGAUGCGGUUCUCAUU UCUUCACCUCCGCAGACGACUCGCCCGA 2425. _H04
AATGTTCTTCTCTTTGTCCG GGGAGGACGAUGCGGAAUGUUCUUCUCUUUGUCCGCAGACGACUCGCCCGA 2426. H04
TTTGGGAGTAGGATGTTCCG GGGAGGACGAUGCGGUU UGGGAGUAGGAUGUUCCGCAGACGAC'JCGCCCGA 2427. ,H04
COTTCTTTTCTTTCCC.GCC GGGAGGACGAUGCGGCCUUUCUU UUCUUUCCCGCCCAGACGACUCGCCCGA 2428. .H04
TCTTTGTAACCTTCTTTCCG GGGAGGACGAUGCGGUCUUUGUAACCUUCUUUCCGCAGACGACUCGCCCGA 2429. H04
GATCATTTTACTCTTCTCCC GGGAGGACGAUGCGGGAUCAUUUUACUCUUCUCCCCAGACGACUCGCCCGA 2430. ,K04
ACTCTTTCGTATTCTTTCCG GGGAGGACGAUGCGGACUCUUUCG UAUUCUUUCCGCAGACGACUCGCCCGA 2431. .H0
CTCTTTTATGTCCCTTTCCG GGGAGGACGAUGCGGCUCUUUUAUGUCCCUUUCCGCAGACGACUCGCCCGA 2432. H04
TCTTTTGCTGT7TTCATCCG GGGAGGACGAUGCGGUCUUUUGCUGUUUUCAUCCGCAGACGACUGGCCCGA 2433. .H04
TACTCATCTTACTTCTTTCCG GGGAGGACGAUGCGGUACUCAUCU UACUUCUUUCCGCAGACGACUCGCCCGA 2434. _H04
TTTCCTATCTT TTCTTCCG GGGAGGACGAUGCGGUU UCCUAUCUUGUUCUUCCGCAGACGACUCGCCCGA 2435. H04
U in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TATTTT CCTTTCTTGTGCC GGGAGGACGAUGCGGUAUUUUUCCUUUCUUGUGCCCAGACGACUCGCCCGA 2436_ .H04
TCTGTTTTTATTGTTTGCCG GGGAGGACGAUGCGGUCUGUUUUUAUUGUUUGCCGCAGACGACUCGCCCGA 2437_ _H04
TTTTTGTTTCACCTTTTCCC GGGAGGACGAUGCGGUUUUUCUUUCACCUUUUCCCCAGACGACUCGCCCGA 2438. _H04
TTCCTTACTTTTCC CTTCCG GGGAGGACGAUGCGGUUCCUUACUUUUCCCUUCCGCAGACGACUCGC C6A 2439. .H04
GCTTTTTTCTTTTCCCGCCG GGGAGGACGAUGCGGGCUUUUUUCU UUUCCCGCCGCAGACGACUCGCCCGA 2440. .H04
TCTTGTTTG GTCTTTCTCCG GGGAGGACGAUGCGGUCUUGUUUGG UCUUUCUCCGCAGACGACUCGCCCGA 2442_ .H04
TATTTTCTTATTTTCGACTG GGGAGGACGAUGCGGUAUUUUCUUAUUUUCGACUGCAGACGACUCGCCCGA 2443_ .H04
TTATTTCTTTG TTTTCG CCC GGGAGGACGAUGCGGUUAU UUCUUUGUUUUCGCCCCAGACGACUCGCCCGA 2445. .H04
ATTCTCTTTCTTTTTCTCC G GGGAGGACGAUGCGGAUUCUCUUUCUUUUUCUCCGCAGACGACUCGCCCGA 2447. .H04
TTTTCGTTTT I ! I I I I GCGCCG GGGAGGACGAUGCGGUUUUCGUUU U UUUUUUGCGCCGCAGACGACUCGCCCGA 2448. _HG4
CCTTCATTCATTCTCTTCCG GGGAGGACGAUGCGGCCUUCAUUCAUUCUCUUCCGCAGACGACUCGCCCGA 2449. _H04
ATAGTTTTGTTTTTTTCGCCG GGGAGGACGAUGCGGAUAGU UU UG UUUUUU UCGCCGCAGACGACUCGCCCGA 2451. _H04
ACTTCTCATCTTTTTCTCCG GGGAGGACGAUGCGGACUUCUCAUCUU UUUCUCCGCAGAC6ACUCGCCCGA 2452. .H04
T6TTTTTACTCTTGCTTCCG GGGAGGACGAUGCGGUGUU UUUACUCUUGCUUCCGCAGACGACUCGCCCGA 2453. .H04
TTTTTGTTAGCTTTCC'CCCG GGGAGGACGAUGCGGUUUUUGUUAGCUUUCCCCCGCAGACGACUCGCCCGA 2454. .H04
TGCTTTGGTTTTTTTTTCCG GGGAGGACGAUGCGGUGCUU UGGUUUUUUU UUCCGCAGACGACUCGCCCGA 2455. .H04
TTTTCTTTATCTA7TCG CCG GGGAGGACGAUGCGGUUUUCUU UAUCUAU UCGCCGCAGACGACUCGCCCGA 2456. .H04
ATCTTATTTCTATTTTGCCG GGGAGGACGAUGCGGAUCUUAUUUCUAUUUUGCCGCAGACGACUCGCCCGA 2457. _H04
CTTCTCGTTTTTTTCTACCG GGGAGGACGAUGCGGCUUCUCGU UUUUUUCUACCGCAGACGACUCGCCCGA 2459. _H04
CCTTT AACGATTCTTTTCCG GGGAGGACGAUGCGGCCUUUAACGAUUCUU UUCCGCAGACGACUCGCCCGA 2460. _H04
TTCTTTTTGTTTTCATGCCG GGGAGGACGAUGCGGUUCUUUUUGUUUUCAUGCCGCAGACGACUCGCCCGA 2461. .H04
ATTTTCTTCTTCT CTTA CC G GGGAGGACGAUGCGGAUUUUCUUCUUCUCUUACCGCAGACGACUCGCCCGA 2462. .HQ4
CTTTCATG CTTCCTTTACCG GGGAGGACGAUGCGGCU U JCAUGCUUCCUU UACCGCAGACGACUCGCCCGA 2463. _H04
TTTATCCTATTTCTCGTCCG GGGAGGACGAUGCGG UUUAUCCUAUUUCUCGUCCGCAGACGACUCGCCCGA 2464. _HG4
CTTTATTAC G TTTCTTTC CG GGGAGGACGAUGCGGCLIU JAUUACGUUUCU UUCCGCAGACGACUCGCCCGA 2466. .H04
Figure imgf000232_0001
GGGAGGACGAUGCGGUUGUUAUUCUUUUUUUUGCCCCAGACGACUCGCCCGA 2467. _H04
ACCTTTTTAATTTTTCTCCG 6GGAG6ACGAUGCGGACCUUUUUAAUUUUUCUCC6CAGACGACUCGCCCGA 2468. .H04
TTTGTCTCTTATTTTACCCG GGGAGGACGAUGCGG'JUUGUCUCUUAUUUUACCCGCAGACGACUCGCCCGA 2469 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTTTATTTTCTTGTTCCTG GGGAGGACGAUGCGGUUUUUAUUUUCUUGUUCCUGCAGACGACUCGCCCGA 2471. JHG4
GTTTTTTAATTTTTGTTCCC GGGAGGACGAUGCGGGUUUUU UAAUUUUUGUUCCCCAGACGACUCGCCCGA 2472. _H04
TCATTTG GTTTTT TATCCG GGGAGGACGAUGCGGUCAU U UGGUUUUUUUAUCCGCAGACGACUCGCCCGA 2473_ _H04
TTCCACTTTT'I ! l"i 1 1 ! CTCCG GGGAGGACGAUGCGGU UCCACUUUUUUUUUUUCUCCGCAGACGACUCGCCCGA 2474. _H04
TTACT TCTTTTTCTACCC GGGAGGACGAUGCGGU UACUU UUCUUUUUCUACCCCAGACGACUCGCCCGA 2476. .H04
CTTT7TATCTATCTTTACCG GGGAGGACGAUGCGGCU UUUUAUCUAUCUUUACCGCAGACGACUCGCCCGA 2477. .H04
AACACTTTTTTATTCTTACTG GGGAGGACGAUGCGGAACACUUU UUUAUUCUUACUGCAGAC.GACUCGCCC6A 2478. .H04
TCGCTTTATTTACTTTTCCC GGGAGGACGAUGCGGUCGCU UUAUUUACUUUUCCCCAGACGACUCGCCCGA 2479. .H04
TCTTTTACTA ATTTAC CCCG GGGAGGACGAUGCGGUCUU UUACUAAUUUACCCCGCAGACGACUCGCCCGA 2480. .H04
TGTTTTATTTGTTTTTTTCCC GGGAGGACGAUGCGGUGUUUUAUUUGU UUUUU UCCCCAGACGACUCGCCCGA 2481. _H04
TTTGTTTTTCTCTTTCCG GGGAGGACGAUGCGGUUUGUUUUUCUCUUUCCGCAGACGACUCGCCCGA 2482. .H04
TTTGTTTTATTTTTTTTGTCCC GG GAGG ACGA UGCGG UUUGUUU UAU U U UU U U U GUCCCCAG ACG ACU CGCCCGA 2483. .H04
TATTTACG i I I I I \ I I GTG GGGAGGACGAUGCGGUAUU UACGUUUUU UUUGUGCAGACGACUCGCCCGA 2484. _H04
TTTCTTCT1 TCTTTTCCG GGGAGGACGAUGCGGUUUCUUCUUUCUUUUCCGCAGACGACUCGCCCGA 2485. _H04
TTTTATCTCTGTTrTTTTGCC GGGAGGACGAUGCGGUUUUAUCUCUGU UUUUUUGCCCAGACGACUCGCCCGA 2486. _H04
TCCTGTCGTCTGTTCGTGCCC GGGAGGACGAUGCGGUCCUG UCGUCUG UUCGUGCCCCAGACGACUCGCCCGA 2487. .H04
ATCTTTTCTTTCTTGCCG GGGAGGACGAUGCGGAUCUUUUCUUUCUUGCC6CAGACGACUCGCCCGA 2489, ,H04
ATTCGGTC I M i l l I GCTCCG GGGAGGACGAUGCGGAU UCGGUCUU UUUUUGCUCCGCAGACGACUCGCCCGA 2490. .H04
TTTTTAG TTTTCTTGTTCCG GGGAGGACGAUGCGGU U UUUAGUUUUCUUGUUCCGCAGACGACUCGCCCGA 2493. _H04
ATTCACTTTATTCCCCACCG 6GGAGGACGAUGCG6AU UCACUUUAUUCCCCACCGCAGACGACUCGCCCGA 2495. _H04
CTTTTCC M i l l ! GTTCCG GGGAGGACGAUGC6GCU UUUCCUUUUUUGUUCCGCAGACGACUCGCCCGA 2496. .H04
G TTTTG TTCTTGTG TTTCCG GGGAGGACGAUGCGGGUUU UGUUCUUGUGU UUCCGCAGACGACUCGCCCGA 2497. Q4
ATCTTTTCTTTCCTTTG CCG GGGAGGACGAUGCGGAUCUU UUCUUUCCUUUGCCGCAGACGACUCGCCCGA 2498. ..H04
TCACATTTTTCTCTCGTCCC GGGAGGACGAUGCGGUCACAUUUUUCUCUCGUCCCCAGACGACUCGCCCGA 2499. .H04
TACTTACTTTATCrTCTCCG GGGAGGAC6AUGCGGUACUUACUUUAUCUUCUCCGCA6ACGACUCGCCCGA 2500. .H04
AATCCTTTTCCTTCTATCCG GGGAGGACGAU6CGGAAUCCU UUUCCUUCUAUCCGCAGACGACUCGCCCGA 2501. .H04
TrTTTTGATTCTTGTTCCG GGGAGGACGAUGCGGUU UUUUGAUUCULIGUUCCGCAGACGACUCGCCCGA 2502. -H04
TCTTATCACGTTCTTTTCCG GGGAGGACGAUGCGG UCU UAUCACGUUCUU UUCCGCAGACGACUCGCCCGA 2503 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
CTTTCACAACTCTCTCTCCG GGGAGGACGAUGCGGCUUUCACAACUCUCUCUCCGCAGACGACUCGCCCGA 2504. _H04
TCTTTTTTTCATTTCICACCG GGGAGGACGAUGCGGUCUU UUUUUCAUU UCUCACCGCAGACGACUCGCCCGA 2505. .H04
TGCTCTTGTTTTCCTTTCCG GGGAGGACGAUGCGGUGCUCUUGUUUUCCUUUCCGCAGACGACUCGCCCGA 2506. .H04
GTTCTACTATTTCTTCACCG GGGAGGACGAUGCGGGUUCUACUAU U UCUUCACCGCAGACGACUCGCCCGA 2507_ _H04
AC CTcmcT TCTGCCG GGGA6GACGAUGCGGACUCUCUU UCUUUUCUGCCGCAGACGACUCGCCCGA 2508. _H04
CC1 ΓΑ A CTACCTTTCCG GGGAGGACGAUGCGGCCUUU UUAACUACCUUUCCGCAGACGACUCGCCCGA 2509. H04
TCCATGCTTTTTTTTTG CCG GGGAGGACGAUGCGGUCCAUGCUUUUUUUUUGCCGCAGACGACUCGCCCGA 2510. _H04
CCT CTTTTTCCCTATCCG GGGAGGACGAUGCGGCCUUUCUUUUUCCCUAUCCGCAGACGACUCGCCCGA 2511. .H04
GTTCATG rrci CCACCG GGGAGGACGAUGCGGGUUCAUGUUUCUUUCCACCGCAGACGACUCGCCCGA 2512. .H04
ATTTCCCTCTTTCATTACCG GGGAGGACGAUGCGGAUUUCCCUCUUUCAUUACCGCAGACGACUCGCCCGA 2514. _H04
ATTTCTCTTCTTCTTGCCG GGGAGGACGAUGCGGAUUUCUCUUCUUCUUGCCGCAGACGACUCGCCCGA 2515. „H04
AATTTCTCTC6CTCTATCCG GGGAGGACGAUGCGGAAUU UCUCUCGCUCUAUCCGCAGACGACUCGCCCGA 2516. .H04
TC I ! I I I GTTCTTTCTTG CC GGGAGGACGAUGCGGUCUU UUUGU UCUUUCUUGCCCAGACGACUCGCCCGA 2517. _H04
TTTTTTCCTTAATTTGTCCG GGGAGGACGAUGCGGUU UUUUCCUUAAUUUGUCCGCAGACGACUCGCCCGA 2518. .H04
ATTTTTAAGTTGTTTTTGCC GGGAGGACGAUGCGGAUUUUUAAGUUGUUUUUGCCCAGACGACUCGCCCGA 2519. .H04
TTCTTTTTGTCATTCTTCTG GGGAGGACGAUGCGGUUCUUUUUGUCAUUCU UCUGCAGACGACUCGCCCGA 2520. _H04
ATACTTTA CCTTTTTTTG CC GGGAGGACGAUGCGGAUACUUUACCUUUUUUUGCCCAGACGACUCGCCCGA 2521. .ΗΌ4
CCATTTCTTTTCTTACTCCG GGGAGGACGAUGCGGCCAUU UCUUUUCUUACUCCGCAGACGACUCGCCC6A 2522. H04
TTCTCTATT ATCTTTG TCCG GGGAGGACGAUGCGGUUCUCUAU UAUCUUUGUCCGCAGACGACUCGCCCGA 2523. _H04
ACTTTATTCTCTTTTTTTCCG GGGAGGACGAUGCGGACULiUAUUCUCIJ UUUU U JCCGCAGACGACUCGCCCGA 2524. .H04
ATTTTTTTGTTGTTTTGACCG GGGAGGACGAUGCGGAUUU UUUUG U UGU UU UGACCGCAGACGACUCGCCCGA 2525. .H04
TTTTCTmGTTTTTTTGTG GGGAGGACGAUGCGGUUUUCUUUUGUUUUUUUGUGCAGAC ACUCGCCCGA 2526. .H04
TT ATTCTTTTG TTTTTACG C GGGAGGACGAUGCGGUUAU UCU UUUGUUUUUACGCCAGACGACUCGCCCGA 2527. .H04
TTACTTG GTTATTTTTTCTG GGGAGGACGAUGCGGUUACU UGGUUAUUUUU UCUGCAGACGACUCGCCCGA 2528. _H04
TTTTTGCTTTATCTGTCCCG GGGAGGACGAUGCGGUU UUUGCUU UAUCUGUCCCGCAGACGACUCGCCCGA 2529. _H04
TTCATTTTCT ATTCTTTG CC GGGAGGACGAUGCGGUUCAUUUUCUAUUCUUUGCCCAGACGACUCGCCCGA 2530. .H04
TTCTTTTTCTTCTATTCCCG GGGAGGACGAUGCGGUUCUUUUUCUUCUAUUCCCGCAGACGACUCGCCCGA 2531. .H04
CTTTCTCTTATTCTTCCCCG GGGAGGACGAUGCGGCUUUCUCUUAUUCUUCCCCGCAGACGACUCGCCCGA 2532 HD4
# in H4
HTS read variable region RNA sequences # in H3 selection se Section Selected sequenc
TTTTTACTG CTTTTT ATCCG GGGAGGACGAUGCGGUUUUUACUGCUUUUUAUCCGCAGACGACUCGCCCGA 2533. _H04
TCACTTTCATATTTC TCCG GGGAGGACGAUGCGGUCACUUUCAUAUUUCUUCCGCAGACGACUCGCCCGA 2534. .H04
TTTTTTTTTCTTGTTTCCG GGGAGGACGAUGCGGUUUUUUUUUCUUGU UUCCGCAGACGACUCGCCCGA 2535, _H04
TCTTTCAGCTCTTTTTCCCG GGGAGGACGAUGCG6UCUUUCAGCUCUUU UUCCCGCAGACGACUCGCCCGA 2536. .H04
CCTTGTTTGTTTTTTTTTCCCG GGGAGGACGAUGCGGCCUUGUUUG U UUUUU UUUCCCGCAGACGACUCGCCCGA 2537.. .H04
TTTCTG CTATTTTTCTTCCG GGGAGGACGAUGCGGUUUCUGCUAUUUUUCU UCCGCAGACGACUCGCCCGA 2538. _H04
AATTTATTATTTCTCCG CCG GGGAGGACGAUGCGGAAUUUAUUAUUUCUCCGCCGCAGACGACUCGCCCGA 2539. _H04
T I G T ! ΑΤΠ A I T IGTTTGCC GGGAGGACGAUGCGGUUGU UAUUUAUUUG UU UGCCCAGACGACUCGCCCGA 2540. _H04
CTTTTTTAATTACCCTTCCG GGGAGGACGAUGCGGCU UUUUUAAUUACCCUUCCGCAGACGACUCGCCCGA 2541. _H04
CTTATTTAT'CTTGTTTTCTG GGGAGGACGAUGCGGCU UAUUUAUCUUG UUUUCUGCAGACGACUCGCCCGA 2542. .H04
CACTTTC ATTTTCTTTTC G C GGGAGGACGAUGCGGCACUU'JCAUUUUCUUUUCGCCAGACGACUCGCCCGA 2543. .H04
CC CTTATTTATTCG CTTCCG GGGAGGACGAUGCGGCCCUUAUUUAUUCGCUUCCGCAGACGACUCGCCCGA 2544. _H04
TCTTTAT'l'TTTTTCCTTTCCG GGGAGGACGAUGCGGUCUU UAUUUU UUUCCUUUCCGCAGACGACUCGCCCGA 2545. .H04
TATTTCACATCTCTTTTCGG GGGAGGACGAUGCGGUAUU UCACAUCUCUUUU LGCAGACGACUCGCCCGA 2546. .H04
TCTTTTACTTTCITATCCTG GGGAGGACGAUGCGGUCUUUUACUUUCUUAUCCUGCAGACGACUCGCCCGA 2547. .H04
TTT ATTTT AC GTTTG TTCTG GGGAGGACGAUGCGGUUUAUUUUACGUUUGUUCUGCAGACGACUCGCCCGA 2548. .H04
CTTTTTTTCC'riTTTTTC CG GGGAGGACGAUGCGGCUUUUUUUCCUUU UUUUCCGCAGACGACUCGCCCGA 2549. HQ4
TCCGTTTCTTTCTTTCCCCG GGGAGGACGAUGCGGUCCGU UUCUUUCUUUCCCCGCAGACGACUCGCCCGA 2550. H04
TTTTTTACCAACCTTTTCCG GGGAGGACGAUGCGGU UUUUUACCAACCUULJUCCGCAGACGACUCGCCCGA 2551. .H04
TTTATTTTTTTTTTCGCTCCG GG'GAGGACGAUGCGGUUUAUUUUUUUUUUCGCUCCGCAGACGACUCGCCCGA 2552. _H04
TTT CATTTTTTTT ATTCTC CG GGGAGGACGAUGCGGUUUCAUU UUUUUUAUUCUCCGCAGACGACUCGCCCGA 2553. .H04
TACTTTAATCTTTTTTTTCCG GGGAGGACGAUGCGGUACUUUAAUCUUUU UUUUCCGCAGACGACUCGCCCGA 2S54. _H04
ACATTTTCTCTTTTTCTGC6 GGGAGGACGAUGCGGACAUU UUCUCUUUU UCUGCGCAGACGACUCGCCCGA 2555. _H04
ΤΑΤΤΤΑ Ϊ I I 1 1 i ! CTTGCCCCG GGGAGGACGAUGCGGUAUUUAUUU UUUUCU UGCCCCGCAGACGACUCGCCCGA 2556. .H04
ATTTTTTGTCT rTGTTACTG GGGAGGACGAUGCGGAUUUUUUGUCUUUUGUUACUGCAGACGACUCGCCCGA 2557. .H04
TTTTTCATACCTTTTTCCG GGGAGGACGAUGCGGUU UUUCAUACCUUUUUCCGCAQACGACUCGCCCGA 2558. _H04
τττο ΰπΏτττττττετσ GGGAGGACGAUGCGGUU UGUUGUUGUUUU UUUCUGCAGACGACUCGCCCGA 2559. _H04
CTTTTCATTTClTiT TTCCG GGGAGGACGAUGCGGCUUUUCAUUUCUUUUUUUCCGCAGACGACUCGCCCGA 2560. _H04
if
HTS read variable region RNA sequences 3 selection selection Selected sequenc T'CTTG "ITTTTTTTG G CCC GGGAGGACGAUGCGGUCUUGUUU UUUUUGGCCCCAGACGACUCGCCCGA 2554JH04
TG TG TCTTTTCTTTTCTG C CC GGGAGGACGAUGCGGUGUGUCUUUUCUUUUCUGCCCCAGAC6ACUCGCCCGA 2565_H04
TCCCTTCGTTCTGCCCTCCC G6GAGGACGAUGCGGUCCCUUCG UUCU6CCCUCCCCAGACGACUCGCCCGA 2566_H04
TCTTATT7TCTTTTG TCCCC GGGAGGACGAUGCGGUCUUAUUUUCUUUUG UCCCCCAGACGACUCGCCCGA 2568_H04
CACCTTTTCTTTTCCGCTCCG GGGAGGACGAUGCGGCACCUUUUCUUUUCCGCUCCGCAGACGACUCGCCCGA 2570_H04
ATTCCTTTTCTTCTTTGCCG GGGAGGACGAUGCGGAUUCCUUUUCUUCU UUGCCGCAGACGACUCGCCCGA 2571_H04
TTCTTCGTATTCTCTTGCCG GGGAGGACGAUGCGGU UCUUCGUAUUCUCUUGCCGCAGACGACUCGCCCGA ■2572_H04
TTTTCGTTi Π I H GCGCCG GGGAGGACGAUGCGGUUUUCGUUUUUU UUGCGCCGCAGACGACUCGCCCGA 2573_H04
TATCTGT CTCTTTTT C G CC G GGGAGGACGAUGCGGUAUCUGUCUCUUUUUCGCCGCAGACGACUCGCCCGA 2574_ H04
CCTGTTTATTTTTTTGCCCCC GGGAGGACGAUGCGGCCUGU UUAUUUUU UUGCCCCCCAGACGACUCGCCCGA 2575_H04
AGATTTGTT iTTTGTGGCCG GGGAGGACGAUGCGGAGAUUUGUUUUUUUGUGGCCGCAGACGACUCGCCCGA 2576_H04
TTCTT GGCTTATTCTCCG GGGAGGACGAUGCGGUUCUUUUGGCUUAUUCUCCGCAGACGACUCGCCCGA 2577_H04
ATCTTTTCTTCTTCTGCCG GGGAGGACGAUGCGGAUCUUUUCUUCUUCUGCCGCAGACGACUCGCCCGA 2578_H04
GTTG CTTTTTTCTATTCCCC GGGAGGACGAUGCGGGUUGCUUUUUUCUAU UCCCCCAGACGACUCGCCCGA 2580_H04
TTTTACTCATTTTTCCCGTG GGGAGGACGAUGCGGU UUUACUCAU UUUUCCCGUGCAGACGACUCGCCCGA 2581 .HG4
TGTTTTGTTTT TTTTG TCCCC GGGAGGACGAUGCGGUGUUUUGUUUUUUUUG UCCCCCAGACGACUCGCCCGA 2582_H04
ATG CTCTCTTTTTCTTGCCG G6GAGGACGAUGCGGAUGCUCUCUUUUUCUUGCCGCAGACGACUCGCCCGA 2583JH04
TTTTCTTTGTCTTCTTGCCG GGGAGGACGAUGCGGUUUUCUUUGUCUUCUUGCCGCAGACGACUCGCCCGA 2584_H04
ACCTTATTTTATCTTCTCCG GGGAGGACGAUGCGGACCUUAUUUUAUCUUCUCCGCAGACGACUCGCCCGA 2585_H04
ATTATCTAATTCTTTCACCG GGGAGGACGAUGCGGALJ UAUCUAAUUCliU UCACCGCAGACGACUCGCCCGA 2586JHQ4
CTTTG CTTATTTTTTCTCCG GGGAGGACGAUGCGGCU UUGCU UAUUUUUUCUCCGCAGACGACUCGCCCGA 2587„H04
TTATTATCCTTTTTCTCCCG GGGAGGACGAUGCGGUUAU UAUCCUUUUUCUCCCGCAGACGACUCGCCCGA 2588JH04
AATTTTCCCCTCTnTACCG GGGAGGACGAUGCGGAAUUUUCCCCUCU UUUACCGCAGACGACUCGCCCGA 2589_H04
TCTTCATGGTTATTTTTCCG GGGAGGACGAUGCGGUCUUCAUGGU UAUUUU UCCGCAGACGACUCGCCCGA 2590_HQ4
TCTTTTCTCTATCTATCCG GGGAGGACGAUGCGGUCUU U UCUCUAUCUAUCCGCAGACGACUCGCCCGA 2591_H04
ATGTTTCTCTTCTCTTTCCG GGGAGGACGAUGCGGAUGUUUCUCU UCUGUUUCCGCAGACGACUCGCCCGA 2592 H04
CTATTCCTATTCCCTTTCCG GGGAGGACGAUGCGGCUAUUCCUAUUCCCUUUCCGCAGACGACUCGCCCGA 2593_H04
TTTTCTTATETCTCTACCCG GGGAGGACGAUGCGGU UUUCUUAUUUCUCUACCCGCAGACGACUCGCCCGA 2597 H04
# in
H7S read variable region RNA sequences 3 selection selection Selected sequenc
ATTG 6 AG GAG G AG GTTCCG GGGAGGACGAUGCGGAUUGGAGGAGGAGGUUCCGCAGACGACUCGCCCGA 2598_ H04
TCTT C ACTAG CTTTTTCCG GGGAGGACGAUGCGGUCUUUCACUAGCUUUUUCCGCAGACGACUCGCCCGA 2599 JH 04
ATTTCTTCTTCTTTTACCG GGGAGGACGAUGCGGAUUUCUUCUUCUUUUACCGCAGACGACUCGCCCGA 2600_HQ4
OTTTTmcTTTCTCCGc GGGAGGACGAUGCGGCU UU UUCUUCUUUCUCCGCCAGACGACUCGCCCGA 2602J404
TTTcrrrrG TATTTTATCCC GGGAGGACGAUGCGGUUUCUUUUGUAUUUUAUCCCCAGACGACUCGCCCGA 2603_H04
TTTCTTTGTTT CCTG CCG C GGGAGGACGAUGCGGU UUCUU UGUUUUCCUGCCGCCAGACGACUCGCCCGA 2604_H04
GCCnCG CTGCCTCCC GGGAGGACGAUGCGGGCCUUCGUUCUGCCUCCCCAGACGACUCGCCCGA 2605„HQ4
TTCTTTTTATTCTACATCCG GGGAGGACGAUGCGGU UCUUU UUAUUCUACAUCCGCAGACGACUCGCCCGA 2606_H04
TTTTCTG GTTTTT ACTTCCG GGGAGGACGAUGCGGUUUUCUGGUUUUUACUUCCGCAGACGACUCGCCCGA 2607_H04
ATTTTCTCATTTCTCTACCC GGGAGGACGAUGCGGAUUUUCUCAUUUCUCUACCCCAGACGACUCGCCCGA 2608_H04
TTTTCTCTATGTTTTATCCC GGGAGGACGAUGCGGU'JUUCUCUAUGUUUUAUCCCCAGACGACUCGCCCGA 2609_H04
TATTCTTTCTTTCTTTTCGC GGGAGGACGAUGCGGUAUUCUUUCUUUCUUU UCGCCAGACGACUCGCCCGA 2610_H04
CCTGTTTTTCTCGTTTTCCG GGGAGGACGAUGCGGCCUGU UUUUCUCG UUUUCCGCAGACGACUCGCCCGA 2611„H04
TTTGG CTCTCTTATTTTCCG GGGAGGACGAUGCGGUUUGGCUCUCUUAU UUUCCGCAGACGACUCGCCCGA 2612.. H04
GTTTAC TTAACTTTTTCCC GGGAGGACGAUGCGGGUUUACU UUAACUUUUUCCCCAGACGACUCGCCCGA 2613„H04
TTTTTTAATG I I ! ] I i l l CCG GGGAGGACGAUGCGGU UUUUUAAUGUUUUUUUUCCGCAGACGACUCGCCCGA 2614_H04
GTATTTATTTCTTGTTGCTG GGGAGGACGAUGCGGGUAU UUAUUUCUUGUUGCUGCAGACGACUCGCCCGA 261S_H04
TTTTCTATCTTTTTTTG TC CG GGGAGGACGAUGCGGUUUUCUAUCUUUUUUUGUCCGCAGACGACUCGCCCGA 2616JH04
TTTTTTCTTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUUUUCUUUUCCUUUGUCCGCAGACGACUCGCCCGA 2617_H04
TTTGTTTTTCTCTTTTTTCCG GGGAGGACGAUGCGGUUUGUU UUUCUCUUU UUUCCGCAGACGACUCGCCCGA 2618J-I04
TTTTAGCTTC I I I ! T S I GTCCT GGGAGGAC6AUGCGGUUUUAGCUUCUUUUUUUGUCCUCAGACGACUCGCCCGA 2619„H04
ATTCCTATTTTCTTCTCCCG GGGAGGACGAUGCGGAUUCCUAU UUUCUUCUCCCGCAGACGACUCGCCCGA 2620JHD4
CTTTTCTCGTACTATTTCCG GGGAGGACGAUGCGGCU UUUCUCGUACUAUUUCCGCAGACGACUCGCCCGA 2622JH04
TTTTACATTCATTTTTTGCGC GGGAGGACGAUGCGGUUUUACAUUCAUUU UU UGCGCCAGACGACUCGCCCGA 2624_H04
TTTTATTTATTCTCTCTCCG GGGAGGACGAUGCGGUUUUAUUUAUUCUCUCUCCGCAGACGACUCGCCCGA 2625 H04
CTTTATATCTATTTTCTCCG GGGAGGACGAUGCGGCUUUAUAUCUAUU UUCUCCGCAGACGACUCGCCCGA 2626_HQ4
ΤΓΤ n i CTTGTTGCTTGCTG GGGAGGACGAUGCGGUUUUUUCUUGUUGCUUGCUGCAGACGACUCGCCCGA 2627_H04
GTTCTTATTTTCTTCCCCTG GGGAGGACGAUGCGGGUUCUUAUUUUCUUCCCCUGCAGACGACUCGCCCGA 2628 HQ4
# in H4
HTS read variable region RNA sequences # in H3 selection selection Sefected sequenc
TTATTTCTATTCTTTCTCTG GGGAGGACGAUGCGGU UAUUUCUAUUCUUUCUCUGCAGACGACUCGCCCGA 262θ_Η04
TTTTTGTTATCCTTTTACGC GGGAGGACGAUGCGGUUUUUGUUAUCCU UUUACGCCAGACGACUCGCCCGA 263Q_H04
TCTTCTTTTTTCACTTTCCG GGGAGGACGAUGCGGUCUUCUUUUUUCACUUUCCGCAGACGACUCGCCCGA 2631_ H04
TTTTGC I ΓΠΊΤΠΊ I I CCCC GGGAGGACGAUGCGGUUUUGCUUUU UUUUU UUCCCCCAGACGACUCGCCCGA 2632JH04
TCTTTTTTTCCTATTTCTCCG GGGAGGACGAUGCGGUCUUUUUUUCCUAU UUCUCCGCAGACGACUCGCCCGA 2633_H04
TTTCTCTTTTTCTCACCCGC GGGAGGACGAUGCGGUU UCUCUUUUUCUCACCCGCCAGACGACUCGCCCGA 2634JH04
TTATTTCTTTG TTCTTTG CG G6GAGGACGAUGCGGUUAUUUGUUUGUUCUUUGCGCAGACGACUCGCCCGA 2635JH04
TTGTTCTTGTTTTTATTCTG GGGAGGACGAUGCGGUUGUUCUUGUUUUUAUUCUGCAGACGACUCGCCCGA 2636_H04
AGTTACTTTTTACTTTTCrG GGGAGGACGAUGCGGAGUUACUUUUUACUUUUCUGCAGACGACUCGCCCGA 2637„H04
TTTTTATATTTGTGGTTCTG GGGAGGACGAUGCGGUUUU UAUAU UUGUGGUUCUGCAGACGACUCGCCCGA 2638_H04
TCACTTTTA CTTTTTCTG CG GGGAGGACGAUGCGG UCACLiUUUACUUUUUCUGCGCAGACGACUCGCCCGA 2639_H04
TTTATCAAGTTTTTTTTCCG GGGAGGACGAUGCGGUUUAUCAAGUUUUUUU UCCGCAGACGACUCGCCCGA 2640 _H04
TTTGTTTTTCTCrrTTCCG GGGAGGACGAUGCGGUUUGUUUUUCUCUUUUCCGCAGACGACUCGCCCGA 2641_H04
TTTTACT I ! 1 1 n'TVT GTG GGGAGGACGAUGCGGUUUUACUUUUUUUUUUGUGCAGACGACUCGCCCGA 2642_ H04
CTTGTTTCCTTTTTTTCTCCG GGGAGGACGAUGCGGCUUGUUUCCUUUUUUUCUCCGCAGACGACUCGCCCGA 2643„H04
TCTTA 1 I f I ! I CTCTTCTCCG GGGAGGACGAUGCGG UCU UAUUUUUUCUCUUCUCCGCAGACGACUCGCCCGA 2644_H04
TTTCAC ACTTTTTTTTTTG CTG GGGAGGACGAUGCGG UUUCACACUUUUUUUUUUGCUGCAGACGACUCGCCCGA 2645_H04
TCCTGTCGTCTGTCTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUCUCGUCCCCAGACGACUCGCCCGA 2647_H04
TCCTGTCGTCCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCCUGUUCGUCCCCAGACGACUCGCCCGA 264g_H04
AATTATGTGTTTTTTGTTACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUUUGUUACCGCAGACGACUCGCCCGA 2649_ H04
TCCTTTCGTTCTGCCCCCCC GGGAGGACGAUGCGGUCCUUUCGUUCUGCCCCCCCCAGACGACUCGCCCGA 2551_H04
TTTGGGAGTAGGGTGTACCG GGGAGGACGAUGCGGUU UGGGAGUAGGGUGUACCGCAGACGACUCGCCCGA 2S53_H04
GTTTTGTTTTTCCTCGTGCCG GGGAGGACGAUGCG6GUUUUGUUUUUCCUCGUGCCGCAGAC6ACUCGCCCGA 2654._H04
TTTCTT ACTCA CTTTCG CCG GGGAGGACGAUGCGGUU UCUUACUCACUUUCGCCGCAGACGACUCGCCCGA 2655„H04
AATTATGTGTTTTTTTGTACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUUUUGUACCGCAGACGACUCGCCCGA 2656„H04
TCTTGTTTCTTCTTTCTCCG GGGAGGACGAUGCGG UCUUGUUUCUUCUUUCUCCGCAGACGACUCGCCCGA 2657_H04
TVTTACGATCCTTTTTGCCG GGGAGGACGAUGCGGUUUUACGAUCCUUUUUGCCGCAGACGACUCGCCCGA 2658_H04
ATGTTTTTCTCTCTTGACCG GGGAGGACGAUGCGGAUGUUUUUCUCUCU UGACCGCAGACGACUCGCCCGA 2659 H04
n in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTCATTCCATTCTTACTCC6 GGGAGGACGAU6CGGUUCAU UCCAUUCUUACUCCGCAGACGACUCGCCCGA 2660_ .HQ4
TGTATTTTCCCTCTTTTCCG GGGAGGACGAUGCGGUGUAUUUUCCCUCUUUUCCGCAGACGACUCGCCCGA 2661. H04
ATTTCTTTTCCTTTTACCG GGGAGGACGAUGCGGAUUUCUUUUCCUUUUACCGCAGACGACUCGCCCGA 2662. _H04
TTGGGTGTAGGGTGTTCCCG GGGAGGACGAUGCGGUUGGGUGUAGGGUGUUCCCGCAGACGACUCGCCCGA 2664. .H04
CTTTTTTT ATTTTGG CTCCG GGGAGGACGAUGCGGCUUU UUUUAUUUUGGCUCCGCAGACGACUCGCCCGA 2667_ .H04
CTTTCACGTTTCTTCTTCCG GGGAGGACGAUGCGGCUUUCACGUUUCUUCUUCCGCAGACGACUCGCCCGA 2668_ _H04
TACTTCTTGTTTCATCTCCG GGGAGGACGAUGCGGUACUUCUUGUUUCAUCUCCGCAGACGACUCGCCCGA 2669_ _H04
CTTTTCTTTACTCATTACCG GGGAGGACGAUGCGGCUUUUCUUUACUCAUUACCGCAGACGACUCGCCCGA 2670. _H04
ATTTTACTAATTCCTACCG GGGAGGACGAUGCGGAUUUUACLJAAUUCCUUACCGCAGACGACUCGCCCGA 2671. .H04
TTCTATTAAATTTCACTCCG GGGAGGACGAUGCGGUUCUAUUAAAUUUCACUCCGCAGACGACUCGCCCGA 2672. _H04
TTTCCGGGTTTTTT'ITGCCG GGGAGGACGAUGCGGUUUCCGGGUUUUU UUUGCCGCAGACGACUCGCCCGA 2673. _H04
AITTTTCTCTTTACTTG CCG GGGAGGACGAUGCGGAUUU UUCUCUUUACUUGCCGCAGACGACUCGCCCGA 2674. _H04
TACTTTTTTTTTTCCACTCCG GGGAGGACGAUGCGGUACUU UUUUUUUUCCACUCCGCAGACGACUCGCCCGA 2675. _H04
TTTGAAGGGTGCTGGGTCCC GGGAGGACGAUGCGGUUUGAAGGGUGCUGGG UCCCCAGACGACUCGCCCGA 2677. _H04
CTTTTTACATTCTTGCTCCG GGGAGGACGAUGCGGCUUUUUACAUUCUUGCUCCGCAGACGACUCGCCCGA 2678. „H04
CTCTTTCTTTTTTTGCTCGC GGGAGGACGAUGCGGCUCUUUCU UU U'JUUGCUCCCCAGACGACUCGCCCGA 2679. _H04
TTTTATTTTTCCCTATCCCG GGGAGGACGAUGCGGUUUUAUUU UUCCCUAUCCCGCAGACGACUCGCCCGA 2680. _H04
CTTTATACTGCAAGCCGTAC GGGAGGACGAUGCGGCUUUAUACUGCAAGCCGUACCAGACGACUCGCCCGA 2681. .H04
GTATGCTTTCTCTTTTTCCG GGGAGGACGAUGCGGG UAUGCUUUCUCUU UUUCCGCAGACGACUCGCCCGA 2682. .H04
TTTCCmTGCTTACTTCTG GGGAGGACGAUGCGGUUUCCUUUUGCUUACUUCUGCAGACGACUCGCCCGA 2683. _H04
TTC-ATATTTTCACTTTCCCG GGGAGGACGAUGCGGUUCAUAUUUUCACU UUCCCGCAGACGACUCGCCCGA 2684. _H04
CTATATGGAGGAGGTGGAGC GGGAGGACGAUGCGGCUAUAUGGAGGAGGUGGAGCCAGACGACUCGCCCGA 2685. _H04
GCTTTCTCACTTTTTTCCCC GGGAGGACGAUGCGGGCUUUCUCACUUUUUUCCCCCAGACGACUCGCCCGA 2686. _H04
TCCTGCGTCTGTTCGTCCC GGGAGGACGAUGCGGUCCUGCGUCUCU UCGUCCCCAGA GACUCGCCCGA 2687. .H04
CTTTTTGTATCTTCTTTCCG GGGAGGACGAUGCGGCUUUUUGUAUCUUCUUUCCGCAGACGACUCGCCCGA 2689. .H04
TG AG TC ACATTTTf'TTACC CC GGGAGGACGAUGCGGUGAGUCACAUUUUUUUACCCCCAGACGACUCGCCCGA 2690. .H04
ACACTTTTTCCTCTTTTCCC GGGAGGACGAUGCGGACACUUUUUCCUCUUUUCCCCAGACGACUCGCCCGA 2692. _HQ4
TTTCCCTTTACTTTTTTCCG GGGAGGACGAUGCGGUUUCCCUUUACUUUUUUCCGCAGACGACUCGCCCGA 2693 H04
# in M
NTS read variable region RNA sequences # in H3 selection selection Selected sequenc TCTATTTTCTTCTACTTCCG GGGAGGACGAUGCGGUCUAUUUUCUUCUACU UCCGCAGACGACUCGCCCGA 2694. _H04
CTTTATTATTATTCACTCCG GGGAGGACGAUGCGGCUUUAUUAUUAUUCACUCCGCAGACGACUCGCCCGA 2695_ _HG4
TTCTTATCATTCCCTTTCCG GGGAGGACGAUGCGGU UCUUAUCAUUCCCUUUCCGCAGACGACUCGCCCGA 2697. _H04
ATTTATTTTTTTTGCGCCCCG GGGAGGACGAUGCGGAUUUAUUUUUUUUGCGCCCCGCAGACGACUCGCCC6A 26.98. _H04
TTCTTTGTCTTTTG TTTGCC GGGAGGACGAUGCGGU UCUUUGUCUUUUGUUUGCCCAGACGACUCGCCCGA 2699. H04
TTTTTATTTG TACTTTTCG C GGGAGGACGAUGCGGUUUUUAUUUGUACUUUUCGCCAGACGACUCGCCCGA 2700. .H04
ATTATTTCCATTCCTTACC6 GGGAGGACGAUGCGGAUUAUUUCCAUUCCUUACCGCAGACGACUCGCCCGA 2701. _H04
TTATTGC rn ! I f I I GGTCCG GGGAGGACGAUGCGGUUAU UGCUUU UUUUUGGUCCGCAGACGACUCGCCCGA 2702. _H04
TGTTTTTCGTA I "I ! I ί I CCG GGGAGGACGAUGCGGUGUUUU UCGUAUUUUUUCCGCAGACGACUCGCCCGA 2703. _HQ4
CTTTATTATTCTTTTACCG GGGAGGACGAUGCGGCUUUAUUAU UCUU UUACCGCAGACGACUCGCCCGA 2704. _H04
TTATTTGTTTATTTTCTCGC GGGAGGACGAUGCG6UUAUUUGUUUAU UUUCUCGCCAGACGACUCGCCCGA 2705. _HQ4
ATCTACACTTTT7 TTTCCCG GGGAGGACGAUGCGGAUCUACACUUUUUUUUUCCCGCAGACGACUCGCCCGA 2706. JH04
TTTGC I I I I Π I TTTATCCG GGGAGGACGAUGCGGUUUGCUUUUUUU UU UAUCCGCAGACGACUCGCCCGA 2707. _H04
TTTTCTCGCTTCACTTTCCG GGGAGGACGAUGCGGUUUUCUCGCUUCACUUUCCGCAGACGACUCGCCCGA 2708. .H04
CATAGGAGTAGGGAGGTTTG GGGAGGACGAUGC6GCAUAGGAGUAGGGAGGUUUGCAGACGACUCGCCCGA 2709. .H04
ATTTATTTTCTTGGCTGCCG GGGAGGACGAUGCGGAUUUAUUUUCUUGGCUGCCGCAGACGACUCGCCCGA 2710. H04
TCTCTCTACTTCTTTTG CCG GGGAGGACGAUGCGGUCUCUCUAGUUCUUU UGCCGCAGACGACUCGCCCGA 2711. _HG4
GTTCTAATACTCTCTTTCCG GGGAGGACGAUGCGGGUUCUAAUACUCUCUUUCCGCAGACGACUCGCCCGA 2722. .H04
T'T CGTGTTTTCTTTTTG CTG GGGAGGACGAUGCGGUUCG UGUUUUCUUUUUGCUGCAGACGACUCGCCCGA 2713. _H04
TCTTTAT6CTTTTGTCTCCG GGGAGGACGAUGCGGUCUU UAUGCUUUUGUCUCCGCAGACGACUCGCCCGA 2714. _H04
ATTTCTGATTATTTTTACCG GGGA6GACGAUGCGGAU UUCUGAUUAUUU UUACCGCAGACGACUCGCCCGA 2715. _H04
TTTATTTTTG CTGTTTTCCG GGGAGGACGAUGCGGUU UAUUUUUGCUG UU UUCCGCAGACGACUCGCCCGA 2716_ -H04
AATTTACATCTTTTTTGCCG GGGAGGACGAUGCG6AAUUUACAUCUU UUUUGCCGCAGACGACUC6CCCGA 2717. .H04
CCT TATTTCT iTTTTCCC GGGAGGACGAUGCGGCCUUUUAUUUCUUUUUUCGCCAGACGACUCGCCCGA 2718. .H04
TTTTGTTT CTT rCACGCGC GGGAGGACGAUGCGGUUUUGUUUUCUUUCACGCGCCAGACGACUCGCCGGA 2719. .H04
TTCTTATTTCCTTTTCCGCC GGGAGGACGAUGCGGUUCUUAUUUCCUUUUCCGCCCAGACGACUCGCCCGA 2720. _H04
TCTTTGCTTTTCGTTTTCCG GGGAGGACGAUGCGGUCUUUGCUUUUCGU UU UCCGCAGACGACUCGCCCGA 2721. _H04
TTTTTGTATG ATTTTCrCCG GGGAGGACGAUGCGGUUULJ UGUAUGAUUUUCUCCGCAGACGACUCGCCCGA 2722 H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
TTCATTCTG TTTTCTCCTG GGGAGGACGAUGCGGUUCAUUCUGUUUUUCUCCUGCAGACGACUCGCCCGA 2724 _H04
TCTCTTTATTTTCCTACCCG GGGAGGACGAUGCGGUCUCUUUAUUUUCCUACCCGCAGACGACUCGCCCGA 2725_H04
CCTT TACTTTTTTTTTCTCCG GGGAGGACGAUGCGGCCUU UUACUUUUU UUUUCUCCGCAGACGACUCGCCCGA 2726JH04
TTTCTTGTCTATTTTTCCG GGGAGGACGAUGCGGU UUCUUGUCUAUUUU UCCGCAGACGACUCGCCCGA 2727__H04
TTTCACCTTATTCCTTTCTG GGGAGGACGAUGCGGU UUCACCUUAUUCCUUUCUGCAGACGACUCGCCCGA 2728_H04
CTTTTATCTTCTATCCTCCG GGGAGGACGAUGCGGCUUUUAUCUUCUAUCCUCCGCAGACGACUCGCCCGA 2729_H04
CCTTATCTATTTTC TTACCG GGGAGGACGAUGCGGCCUUAUCUAUUUUCUUACCGCAGACGACUCGCCCGA 2730_H04
TTCTTGCTTTA I I i I l l f CCG GGGAGGACGAUGCGGU UCUUGCUU UAUU UUUUUCCGCAGACGACUCGCCCGA 2731„H04
Απατ rCTTATTCCCG GGGAGGACGAUGCGGAUUCUUUUUUCUUAU UCCCGCAGACGAC UCGCCCGA 2732„H04
ATTTCACTF TTTTCCTTCGC GGGAGGACGAUGCGGAUUUCACUUUUUUCCUUCGCCAGACGACUCGCCCGA 2733_H04
ACTTTCATATTTTTTTCACCG GGGAGGACGAUGCGGACUU UCAUAUUU UUUUCACCGCAGACGACUCGCCC6A 2734_H04
CACATTTCTCATTTTATCCG GGGAGGACGAUGCGGCACAU UUCUCAUUUUAUCCGCAGACGACUCGCCCGA 2735_H04
CTTTTTCAAATTTTTCTCCG GGGAGGACGAUGCGGCUUU UUCAAAUUUUUCUCCGCAGACGACUCGCCCGA 2736JHG4
TCATn TATTTCTTTTCGC GGGAGGACGAUGCGGUCAU UUUUAUUUCUUU UCGCCAeACGACUCGCCCGA 2737JH04
TGTTTTCTTCTG FGCG G6GAGGACGAUGCGGUGUUUUCUUCUGUUU UGCGCAGA.C6ACUCGCCCGA 273S. H04
TTTTATTTATTTCCGTCCCG GGGAGGACGAUGCGGUUUUAUUUAUUUCCGUCCCGCAGACGACUCGCCCGA 2739JH04
CATCTTTCTTTATTA CTCCG GGGAGGACGAUGCGGCAUCUUUCUUUAUUACUCCGCAGACGACUCGCCCGA 2740_H04
GATACTTTTTTTT'nTTGCCCC GGGAGGACGAUGCGGGAUACUUU UUUUU UUUUGCCCCCAGACGACUCGCCCGA 2741JHG4
TTAGTGTTTAGTTTTTTTGTG GGGAGGACGAUGCGGU UAG UGUUUAGUUUUUUUG UGCAGACGACUCGCCCGA 2742JH04
ACCTTTTG TTTCTATTTCCG GGGAGGACGAUGCGGACCUUUUGUUUCUAUUUCC6CAGACGACUCGCCCGA 2743._H04
CATTCTCTAATTTTTCTCCG GGGAGGACGAUGCGGCAUUCUCUAAUU UUUCUCCGCAGACGACUCGCCCGA 2744_ H04
TCACTTTATTCTATTCTCTG GGGAGGACGAUGCGGUCACUUUAUUCUAUUCUCUGCAGACGACUCGCCCGA 2745JHG4
TTTGGGGTTTTCCCGAGCCC GGGAGGACGAUGCGGUUUG6GGUUUUCCCGAGCCCCAGACGACUCGCCCGA 2746JH04
TTTG AG GTTTTCCGGTGCCC GGGAGGACGAUGCGGUUUGAGG UUUUCCCGUGCCCCAGACGACUCGCCCGA 2747_H04
TGAGTCGTTCCCTTGTCCC GGGAGGACGAUGCGGUGAGUCG UUCCCUUGUCCCCAGACGACUCGCCCGA 2748_H04
TCCTTGTCGTCCTG CGTCCC GGGAGGACGAUGCGGUCCUUGUCGUCCUGCGUCCCCAGACGACUCGCCCGA 2749_H04
TTTAGGAGTAGG GTGTTCCG GGGAGGACGAUGCGGUU UAGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 2750„H04
TCCTTTCGTCCTGCCCTCCC GGGAGGACGAUGCGGUCCUUUCGUCCUGCCCUCCCCAGACGACUCGCCCGA 2751 H04
# in
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTG G'G AATAG G GTG TTCCG GGGAGGACGAUGCGGU UUGGGAAUAGGGUGUUCCGCAGACGACUCGCCCGA 2752_ JH 04
ATTTCCTTTCTCTGTTGCCG GGGAGGACGAUGCGGAUUUCCUUUCUCUGUUGCCGCAGACGACUCGCCCGA 2754_ .H04
GCTTTCTTCCTGTTTCTCCG GGGAGGACGAUGCGGGCUU UCUUCCUGUUUCUCCGCAGACGACUCGCCCGA 2755. _H04
ATCCGTTTGTTCTTTCTCCG GGGAGGACGAUGCGGAUCCG UU UGUUCUUUCUCCGCAGACGACUCGCCCGA 2756. _H04
ATTTTCTCTTTTTCTG CCG GGGAGGACGAUGCGGAUUU UCUCUUUUUCUGCCGCAGACGACUCGCCCGA 2757. _HG4
CCACTA7TGTCTTTTCTCC6 GGGAGGACGAUGCGGCCACUAUUGUCUUU UCUCCGCAGACGACUCGCCCGA 2758, H04
CCTiTGCTCTTTTCCCTCCG GGGAGGACGAUGCG6CCUUUGCUCUUUUCCCUCCGCAGACGACUCGCCC6A 2760. .H04
TTTTCCGTTCCTTTTTCCG GGGAGGACGAUGCGGU UUUCCG UUCCUUUUUCCGCAGACGACUCGCCCGA 2761. _H04
ATCCTTTATTTTTCTCACGCCG GGGAGGACGAUGCGGAUCCUUUAUUUUUCUCACGCCGCAGACGACUCGCCCGA 2762. .H04
ACTTCTCCTA.CTTTTCTCCG GGGAGGACGAUGCGGACUUCUCCUACUUUUCUCCGCAGACGACUCGCCCGA 2763. _H04
GATTTTAACTTTGCTCTCCC GGGAGGACGAUGCGGGAUUUUAACUUUeCUCUCCCCAGACGACUCGCCCGA 2764. .H04
CCCTTTAATCTTCTTTTCCG GGGAGGACGAUGCGGCCCUUUAAUCU UCUUUUCCGCAGACGACUCGCCCGA 2765. .H04
TTTCTACTCCTTTCA CTCCG GGGAGGACGAUGCGGUUUCUACUCCUUUCACUCCGCAGACGACUCGCCCGA 2766. .H04
ATTTTCTTCTTCTTTACCG GGGAGGACGAUGCGGAU UU UCU UCU UCUU UACCGCAGACGACUCGCCCGA 2767. .H04
CTTTCTCTTTCATG CTTCCG GGGAGGACGAUGCGGCUUUCUCUUUCAUGCUUCCGCAGACGACUCGCCCGA 2768. .H04
GTTGCCTTCACTTTTTTCCG GGGAGGACGAUGCGGGUUGCCU UCACUUUUUUCCGCAGACGACUCGCCCGA 2769. _HG4
CTT ATTCTTTCCG TTTTC CG GGGAGGACCAUGCGGCUUAU UCUUUCCGUUUUCCGCAGACGACUCGCCCGA 2770. .H04
TTTATTTTGGTACCTTTCCG GGGAGGACGAUGCGGUUUAUUUUGGUACCUUUCCGCAGACGACUCGCCCGA 2771. _H04
CTTCGTTATTTCrrCTTCCG GGGAGGACGAUGCGGCUUCG UUAUU UCUUCUUCCGCAGACGACUCGCCCGA 2772. H04
TTTCCG ATTTCTTTTTG CCC GGGAGGACGAUGCGGUUUCCGAUUUCUUUUUGCCCCAGACGACUCGCCCGA 2773. ,H04
CATCTTATT rCTTATCCG GGGAGGACGAUGCGGCAUCU UAUUUUUCUUAUCCGCAGACGACUCGCCCGA 2774. .H04
ATACTCTTTTCCTT TGCC GGGAGGACGAUGCGGAUACUCUUUUCCUUUUUGCCCAGACGACUCGCCCGA 2775. _H04
TTTTTCTCTTTTCTGCTG CC GGGAGGACGAUGCGGUUUU UCUCUUUUCUGCUGCCCAGACGACUCGCCCGA 2776. .H04
TTCTTACTTACTCTTCTCTG GGGAGGACGAUGCGGUUCUUACU UACUCUUCUCUGCAGACGACUCGCCCGA 2777. H04
TGTTTTTGCTATTTCTTGCC GGGAGGACGAUGCGGUGUUUU UGCUAUUUCUUGCCCAGACGACUCGCGCGA 2778. H04
TGATTTATTTGTTTTTGCAC GGGAGGACGAUGCG UGAUUUAUUUGUUUUUGCACCAGACGACUCGCCCGA 2779. .H04
TTCTGTCTTCTTTTTACCCG GGGAGGACGAUGCGG UUCUGUCUUCUUUUUACCCGCAGACGACUCGCCCGA 2780. .H04
ATTTACATCTTTCGCTCCCG GGGAGGACGAUGC6GAUUUACAUCUU UCGCUCCCGCAGACGACUCGCCCGA 2781 H04
8 in H4
HTS read variable region NA sequences 3 selection selection Selected sequenc TATTTATCCTTTCTTCTGTG GGGAGGACGAUGCGGUAUU UAUCCUUUCUUCUG UGCAGACGACUCGCCCGA 27S3_H04
CATTGTTTTA1 TCCCCGC GGGAGGACGAUGCGGCAUUGU UUUAUUUUCCCCGCCAGACGACUCGCCCGA 2784_H04
CCTTTTACTTTTT rrccccG GGGAGGACGAUGCGGCCUUUUACUUUUUUUCCCCGCAGACGACUCGCCCGA 2786 H04
CTTTTTTCATTCCCTTTTGCCG GGGAGGACGAUGCGGCUUUUU UCAUUCCCUUUUGCCGCAGACGACUCGCCCGA 2787_H04
TTTG TTTTTCTCCTTTTCCG GGGAGGACGAU6CGGUUUGUU UU UCUCCUUUUCCGCAGACGACUCGCCCGA 278S_H04
TTCAATTCTTTACTTTTCCC GGGAGGACGAUGCGGUUCAAUUCUUUACU UUUCCCCAGACGACUCGCCCGA 2789...H04
TCTTTTTTTCTTCTACCCCCG GGGAGGACGAUGCGGUCUU UUUUUCUUCUACCCCCGCAGACGACUCGCCCGA 2790_H04
AACTTTTATTGCTTTTACCC GGGAGGACGAUGCGGAACUUUUAUU6CUUUUACCCCAGACGACUCGCCCGA 2791_H04
GATACTTTTTTTTTTTGCCCC GGGAGGACGAUGCGGGAUACUU UUUUUUUUUGCCCCCAGACGACUCGCCCGA 2792 _H04
TC ATACTTTTTTTCG CTCTG GGGAGGACGAUGCGGUGAUACUUUUUUUCGCUCUGCAGACGACUCGCCCGA 2793.. HG4
CCAC G TTTATTTTTTATCCG GGGAGGACGAUGCGGCCACGUUUAUUUUU UAUCCGCAGACGACUCGCCCGA 2794_H04
CTTTTTATGTTCTTTTTCCC GGGAGGACGAUGCGGCU UUUUAUGU UCU UUUUCCCCAGACGACUCGCCCGA 2795JH04
CTTTATTTTCATG CTTACCG GGGAGGACGAUGCGGCUUUAUUUUCAUGCUUACCGCAGACGACUCGCCCGA 2796JHQ4
ATTAACTCTTTCCTTTACCG GGGAGGACGAUGCGGAUUAACUCUUUGCUUUACCGCAGACGACUCGCCCGA 2797_H04
GTTTTTAG TTTTTG TTTCCC GGGAGGACGAUGCGGGUUUUUAGUUUU UGUUUCCCCAGACGACUCGCCCGA 2798_H04
C fTTTTCTTCCTTTTACGC GGGAGGACGAUGCGGCCUUUUUCUUCCUUUUACGCCAGACGACUCGCCCGA 2799_H04
ΑΑπΰττεκττττοΑΤϋοα GGGAGGACGAUGCGGAAUUGUUCUCUUUUCAUCCGCAGACGACUCGCCCGA 2800_H04
TTATTACTTATCTTTCTGTG GGGAGGACGAUGCGGUUAUUACUUAUCUUUCUGUGCAGACGACUCGCCCGA 2801_H04
ATTTTCAGCGTTTTTTCCCG GGGAGGACGAUGCGGAU UUUCAGCGUUUUUUCCCGCAGACGACUCGCCCGA 2802_H04
AATCTTCTCATTTTCGTCCG GGGAGGACGAUGCGGAAUCUUCUCAU UUUCGUCCGCAGACGACUCGCCCGA 2803„HQ4
ATTATACTTTCCTTTTACCC GGGAGGACGAUGCGGAUUAUACUUUCCUUUUACCCCAGACGACUCGCCCGA 2805_H04
CCTTTTCTATACTTCAT'CCG GGGAGGACGAUGCGGCCUUUUCUAUACUUCAUCCeCAGACGACUCGCCCGA 2806_ H04
TTACTTATTGCTTTCTCCCG GGGAGGACGAUGCGGUUACU UAUUCCUUUCUCCCGCAGACGACUCGCCCGA 2807_H04
TTTTfTCACG GTTTTTTTCTG GGGAGGACGAUGCGGUUUU UUCACGGUUUUU UUCUGCAGACGACUCGCCCGA 28Q9_H04
TTTGTTTCTCTCTTTTTCCG GGGAGGACGAUGCGGUUUGUUUCUCUCUUUUUCCGCAGACGACUCGCCCGA 2810_H04
CTCTTCTTG TCTCTTTTCCG GGGAGGACGAUGCGGCUCUUCUUGUCUCUUUUCCGCAGACGACUCGCCCGA 2811 HD4
GTTCTTCTTCTGTTTTTCCG GGGAGGACGAUGCGGGU UCUUCUUCUGUUUUUCCGCAGACGACUCGCCCGA 2812_ HG4
TTACTTTT ATG CTTTTTC CG GGGAGGACGAUGCGGUUACUUUUAUGCUUUU UCCGCAGACGACUCGCCCGA 2813 H04
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# in H4
HIS read variable region RNA sequences selection Selected sequenc
TCATTTG TCTTTATCCTCCG GGGAGGACGAUGCGGUCAUUUGUCUUUAUCCUCCGCAGACGACUCGCCCGA 2844_H04
ATTTTCTCTTTTTTTG CCG GGGAGGACGAUGCGGAUUUUCUCUUUUUUUGCCGCAGACGACUCGCCCGA 2846_H04
TTTTGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUUGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 2847_H04
TTTTTGTTATCTCTCTGCGC GGGAGGACGAUGCGGUUUUUGUUAUCUCUCUGCGCCAGACGACUCGCCCGA 2848_H04
G ATTTCGCTCATTTTTTTG CC GGGAGGACGAUGCGGGAUUUCGCUCAUUU UUUUGCCCAGACGACUCGCCCGA 2849_H04
TTTTCTCGCTTTCTCCGCCG GGGAGGACGAUGCGGUUUUCUCGCUUUCUCCGCCGCAGACGACUCGCCCGA 2850_H04
TCCATGC'I 'Π 'Π Ϊ Ί I !TTGCCG GGGAGGACGAUGCGGUCCAUGCUUUUUUUUUUUGCCGCAGACGACUCGCCCGA 2853_H04
TTACTTTTTTCTT CC TGCCG G G GAG G ACGA UG CG G U U ACU U U U U U CU U CCU 6 CCG CAG ACG AC UCGC CCG A 2854JH04
CTCTTCATACCATTTCTCCG GGGAGGACGAUGCGGCUCUUCAUACCAUUUCUCCGCAGACGACUCGCCCGA 2856_H04
CTTGTGTTTTT TTGCC GGGAGGACGAUGCGGCUUGUGUUUUUUUUUUUGCCCAGACGACUCGCCCGA 2857_H04
TTTCACTTTGAC1 !TTGCC GGGAGGACGAUGCGGUUUCACUUUGACUUUU UGCCCAGACGACUCGCCCGA 2858_H04
GTTCGTGTTTCTTCTTTCCG GGGAGGACGAU6CGGGUUCGUGUUUCUUCUUUCCGCAGACGACUCGCCCGA 2859JH04
TTTTrTTATTG ATTG CTCCG GGGAG6ACGAUGCGGUUUU UUUAUUGAUUGCUCCGCAGACGACUCGCCCGA 2860__H04
ATATTGTCTCTTTTTTTCCG GGGAGGACGAUGCGGAUAUUGUCUCUUUUUUUCCGCAGACGACUCGCCCGA 2861_H04
GTTTTGTTTGTTGTTTCCCG GGGAGGACGAUGCGGGU UUUGUUUGUUGUyuC'CCGCAGACGACUCGCCCGA 2862_H04
TTGGGGTTTTCCCGTGCC6 GGGAGGACGAUGCGGUUGGGGUUUUCCCG UGCCGCAGACGACUCGCCCGA 2S63_H04
TCTTTCACTCTTATTATCCG GGGAGGACGAUGCGGUCUUUCACUCUUAUUAUCCGCAGACGACUCGCCCGA 2865JH04
ATTCCTTTCTCTTTTCACCG GGGAGGACGAUGCGGAUUCCUUUCUCUUUUCACCGCAGACGACUCGCCCGA 2866..H04
TTACTTTCTTTTTTCCCACCC GGGAGGACGAUGCGGUUACUUU-CUUUUUUCCCACCCCAGACGACUCGCCCGA 2867_H04
GTTG CTTTTTATGTTTTACCG GGGAGGACGAUGCGGGUUGCUUUUUAUGUUUUACCGCAGACGACUCGCCCGA 2868_H04
TTTCATCTA7TCTACTTCCG GGGAGGACGAUGCGGUUUCAUCUAUUCUACUUCCGCAGACGACUCGCCCGA 2869_H04
ACTCTTTTTTTGCTTCTTCCG GGGAGGACGAUGCGGACUCUUUUUUUGCUUCU UCCGCAGACGACUCGCCCGA 2870JH04
AGTTTCGTG 1 1 1 ! I f I t GCCC GGGAGGACGAUGCGGAG UUUCGUGUUUUUU ULGCCCCAGACGACUCGCCCGA 2871_H04
TTTTCCGAAGTC i I I I I I CCG GGGAGGACGAUGCGGUUUUCCGAAGUCUUUUUUCCGCAGACGACUCGCCCGA 2872_H04
ATTCCACTTTTTTTTCCACCG GGGAGGACGAUGCGGAUUCCACUUUUUUUUCCACCGCAGACGACUCGCCCGA 2873_H04
TCATTTTTCCA GTTTTTCCC GGGAGGACGAUGCGGUCAUUUUUCCAGUUUUUCCCCAGACGACUCGCCCGA 28 6_H04
ACTTTATTTCCCTTATTCTG GGGAGGACGAUGCGGACUUUAUUUCCCUUAUUCUGCAGACGACUCGCCCGA 2877_H04
TACTATTTTTACTTCCTCCG GGGAGGACGAUGCGG UACUAUUUUUACUUCCUCCGCAGACGACUCGCCCGA 2878_H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
CTTTTCTTTCTCCTTCACCG GGGAGGACGAUGCGGCUUU'JCUUUCUCCUUCACCGCAGACGACUCGCCCGA 2879_H04
TTATTTTTGTTTGTTTGTCC GGGAGGACGAUGCGGUUAUUUUUGUUUG'JUUGUCCCAGACGACUCGCCCGA 2880_H04
TTTTCACTTCATTTTTTTTGCC GGGAGGACGAUGCGGUUUUCACUUCAUUUUU UUUGCCCAGACGACUCGCCCGA 2881_H04
ATTTAATCCTTTCTTCACC GGGAGGACGAUGCGGAUUUAAUCCUUUCUUCACCGCAGACGACUCGCCCGA 2882_H04
CTTCTTTATTCTTTTCTCTG GGGAGGACGAUGCGGCUUCUUUAUUCUUUUCUCUGCAGACGACUCGCCCGA 2883_.H04
GTTTTCCGTGCTCTTTTCCG GGGAGGACGAUGCGGG UUUUCCGUGCUCUU'UUCCGCAGACGACUCGCCCGA 2884_H04
TATTCTTTCTTTTTCAACCt GGGAGGACGAUGCGGUAUUCUUUCUUUUUCAACCCCAGACGACUCGCCCGA 2885_H04
TTTTTAGCTGTCTTTTTCCG GGGAGGACGAUGCGGU UUUUAGCUGUCU UUUUCCGCAGACGACUCGCCCGA 2886_H04
ATTTTTGTTTCGTTTGCCTG GGGAGGACGAUGCGGAULUUUGUU UCGUUUGCCUGCAGACGACUCGCCCGA 2887J-I04
G7TTACTTTTTACTCTGCCG GGGAGGACGAUGCGGGUUUACUUUUUACUCUGCCGCAGACGACUCGCCCGA 2888_H04
ACTTTACTCTTCCTTT ACCG GGGAGGACGAUGCGGACUUUACUCUUCCUUUACCGCAGACGACUCGCCC6A Z890_H04
GTACTATTTCTTTCCTTCCG GGGAGGACGAUGCGGGUACUAUUUCUUUCCUUCCGCAGACGACUCGCCCGA 2891JH04
ACTTTTCCTGACTTATTCCG GGGAGGACGAUGCGGACUU UUCCUGACUUAUUCCGCAGACGACUCGCCCGA 2892JH04
CATTCTTTTTTACTTTTCCC GGGAGGACGAUGCGGCAUUCUUUUUUACUUUUCCCCAGACGACUCGCCCGA 2893_H04
TTTTTGTTACG I I i t n'TTGCG GGGAGGACGAUGCGGUUUUUG UUACGUUUUUUUUGCGCAGACGACUCGCCCGA 2894_H04
GTCCATATTTTCTTCTTCCG GGGAGGACGAUGCGGGUCCAUAUUUUCUUCUUCCGCAGACGACUCGCCCGA 2896_H04
TTATTTTTATTTTCC CTGTA GGGAGGACGAUGCGGUUAUUU UUAUUU UCCCUGUACAGACGACUCGCCCGA 2897_H04
TTTTCATG AAGTTTTTTCCG GGGAGGACGAUGCGGUUUUCAUGAAGUU UUUUCCGCAGACGACUCGCCCGA 2898_H04
ATTTTTTGTTTCCTTTGCCG GGGAGGACGAUGCGGAU UUUUUGUUUCCUU UGCCGCAGACGACUCGCCCGA 2S99_H04
TTTTTCTACTCTTTTTTTCCG GGGAGGACGAUGCGGUUUUUCUACUCUUUUUUUCCGCAGACGACUCGCCCGA 29QQ_H04
CTTTTTT CG TCTCTTTTCCG GGGAGGACGAUGCGGCUUUUUUCGUCUCUUUUCCGCAGACGACUCGCCCGA 2901_ H04
TTATTTTCC! I ; I I iCTGCCG GGGAGGACGAUGCGGUUAU UUUCCUUUU UUCUGCCGCAGACGACUCGCCCGA 2902_H04
TATGTTCTTTTTTTG TCCCG GGGAGGACGAUGCGGUAUGUUCUUUUUUUG UCCCGCAGACGACUCGCCCGA 2903_H04
ATTTTCTTCTTCTTACCG GGGAGGACGAUGCGGAUUU UCUUCUUCUUACCGCAGACGACUCGCCCGA 2904_H04
ATTACTTAATACTTTTTGCC GGGAGGACGAUGCGGAUUACUUAAUACUUUUU6CCCAGACGACUCGCCCGA 290S_H04
CTTTCTCTCTATTTATTCCG GGGAGGACGAUGCGGCUUUCUCUCUAUUUAUUCCGCAGACGACUCGCCCGA 2906_ H04
TTCTAATCATTTCTTTTCCC GGGAGGACGAUGCGGUUCUAAUGAUUUCUUUUCCCCAGACGACUCGCCCGA 2907„H04
TTTTATTTCTTTCCATTCCC GGGAGGACGAUGCGGUU UUAUUUCUUUCCAUUCCCCAGACGACUCGCCCGA 2908_H04
# in H4
NTS read variable region NA sequences selection Selected sequenc
ACTTCTTTATTTTTTCTTCCG GGGAGGACGAUGCGGACUUCUUUAUUUUUUCUUCCGCAGAGGACUCGCCCGA 2909_H04
ATATTCTTTTTTCTCAGCTG GGGAGGACGAUGCGGAUAUUCUUUUUUCUCAGCUGCAGACGACUCGCCCGA 291CLH04
ACATCATTTTCTTTTTACCC GGGAGGACGAUGCGGACAUCAUUUUCUUUUUACCCCAGACGACUCGCCCGA 2911_H04
TTncAcrrrTTTTTCGGCTG GGGAGGACGAUGCGGU UUUCACUU UUUUUUCGGCUGCAGACGACUCGCCCGA 2913..H04
TTTTCCTAG TTTTTTCCCCG GGGAGGACGAUGCGGUUUUCCUAGUUUUUUCCCCGCAGACGACUCGCCCGA 2914_H04
CCTTTTTACTTTCTCTTCCG GGGAGGACGAUGCGGCCUUUUUACUUUCUCUUCCGCAGACGACUCGCCCGA 2915_H04
TTTGTTTTCCTCTTTTTCCG GGGAGGACGAUGCGGUUUGUUUUCCUCUUUUUCCGCAGACGACUCGCCCGA 2916_HG4
CTTTCTTG CTCTCTTTCCCG GGGAGGACGAUGCGGCUUUCUUGCUCUCUUUCCCGCAGACGACUCGCCCGA 2917_H04
TTTTATTACTTCTTGTTCTG GGGAGGACGAUGCGGUUU UAUUACUUCU UGUUCUGCAGACGACUCGCCCGA 2918_HG4
TTTTAATTTTTTCCTTCGTG GGGAGGACGAUGCGGUUUUAAU UUUUUCCUUCGUGCAGACGACUCGCCCGA 2919_H04
GCTTTTTTTCTCTTTCCCTG GGGAGGACGAUGCGGGCUUUUUUUCUCUUUCCCUGCAGACGACUCGCCCGA 2920JH04
TTTTC ACTATTTTTATTCG C GGGAGGACGAUGCGGUUUUCACUAUUUU UAUUCGCCAGACGACUCGCCCGA 2921„H04
TCCTGTCGTCTG CTTGTCCC GGGAGGACGAUGCGGUCCUG UCGUCUGCU UGUCCCCAGACGACUCGCCCGA 2922_H04
CCTTTCG TTCTTCCCTCCC GGGAGGACGAUGCGGCCUUUCGUUCUUCCCUCCCCAGACGAC'JCGCCCGA 2923_H04
TGAGTCGTTCCCCTCGTCCC GGGAGGACGAUGCGGUGAGUCGUUCCCCUCGUCCCCAGACGACUCGCCCGA 2925„H04
GCTTTTCGTTCTACCCTCCC GGGAGGACGAUGCGGGCUUUUCGUUCUACCCUCCCCAGACGACUCGCCCGA 2927_H04
ATTC ACTTTTTCTTG CCG GGGAGGACGAUGGGGAUUCACUU UUUCUUGCCGCAGACGACUCGCCCGA 2929_ H04
CTTTCCTTGCTTTCCCTCCG GGGAGGACGAUGCGGCUUUCCU UGCUUUCCCUCCGCAGACGACUCGCCCGA 2930_HG4
TTTTATTATCTCCGTCTCCG GGGAGGACGAUGCGGUUUUAU UAUCUCCGUCUCCGCAGACGACUCGCCCGA 2931_H04
TTTGAAGGGTGATGTGTCCC GGGAGGACGAUGCG6U UUGA.AGGG UGAUGUGUCCCCA6AGGACUCGCCCGA 2933_M04
TACATTCTTn'TTTCTCTCCG GGGAGGACGAUGCGGUACAUUCUU UUUUUCUCUCCGCAGACGACUCGCCCGA 2934_H04
αΑποτΑΤΑετεοτττεεα GGGAGGACGAUGCGGCUAUUCUAUACUCCUUUCCGCAGACGACUCGCCCGA 2935 _HG4
CACTTCTTTCTCTTATCCG GGGAGGACGAUGCGGCACUUCUUUCUCUUAUCCGCAGACGACUCGCCCGA 2936_ HG4
TCCTTTCGTTCTGCCTCCCC GGGAGGACGAUGCGGUCCUUUCGUUCUGCCUCCCCCAGACGACUCGCCCGA 2937_HG4
T7TTTGTTGTATTGGTTCCG GGGAGGACGAUGCGGU UUU UGUUGUAUL!GGUUCCGCAGACGACUCGCCCGA 2939_H04
CCATCTACTTTCACTTTCCG GGGAGGACGAUGCGGCCAUCUACUUUCACUU UCCGCAGACGACUCGCCCGA 2940_H04
TTTGTTCTTGTTTTTGTCCG GGGAGGACGAUGCGGUUUGUUCUUGUUUUUGUCCGCAGACGACUCGCCCGA 2941 H04
in αι u c
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Figure imgf000248_0001
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Figure imgf000248_0002
# tn H4
HTS read variable region RNA sequences U in H3 selection selection Selected sequenc TATTGTATTTTTTGTTCCCG GGGAGGACGAUGCGGUAU UGUAU UUUUUGUUCCCGCAGACGACUCGCCCGA 29?4_HQ4
TTTCTTTTCATTTTTTCCG GGGAGGACGAUGCGGULf UCUUUUCAUU UUUUCCGCAGACGACUCGCCCGA 297S_HQ4
CCTTTTCTCGCTGTTATCCG GGGAGGACGAUGCGGCCUUUUCUCGCUGUUAUCCGCAGACGACUCGCCCGA 2976_ H04
TTTCTTTCTTCTTGTTCCCG GGGAGGACGAUGCGGUUUCUUUCUUCUUGUUCCCGCAGACGACUCGCCCeA 29?8_H04
TCTATTTCCTTTCTTTCCG GGGAGGACGAUGCGGUCUAUUUCCUUUCUUUCCGCAGACGACUCGCCCGA 298Q_H04
^ΟΤΤΤ< ΑπΑΤΤ6π 6 GGGAGGACGAUGCGGUUUGUUUCCAU UAUUGUCCGCAGACGACUCGCCCGA 2981„Η04
CCCTTTATTTATTTCTACCG GGGAGGACGAUGCGGCCCUUUAUUUAUU UCUACCGCAGACGACUCGCCCGA 2982„H04
TTTCTCTTTCTCTCTTTCCG GGGAGGACGAUGCGGUUUCUCUUUCUCUCUU UCCGCAGACGACUCGCCCGA 2983„H04
TCTCTTTATTTCTTCTTGCG GGGAGGACGAUGCGGUCUCUU UAUUUCUUCUUCCGCAGACGACUCGCCCGA 2985_H04
ττεΑΤτττοΑπτττϋΤΏεσ GGGAGGACGAUGCGGUUCAUUUUCAUUUUUCUGCGCAGACGACUCGCCCGA 2986JH04
TTATCCTTGTTTTTTTATCCC GGGAGGACGAUGCGGUUAUCCUUG UU UUUUUAUCCCCAGACGACUCGCCCGA 2987_H04
TTTTCTCTCTTCATCTTCCG GGGAGGACGAUGCGGUUUUCUCUCUUCAUCUUCCGCAGACGACUCGCCCGA 2988_H04
4- TAGTATGTTTTTGTTTTGTG GGGAGGACGAUGCGGUAGUAUGUUUUUGUUU UGUGCA6ACGACUCGCCCGA 2989_HQ4
TCTTTTCGCTTTACTTACCG GGGAGGACGAUGCGGUCU UUUCGCU UUACUUACCGCAGACGACUCGCCCGA 2990„H04
TATCTATTTACACTTTTCCG GGGAGGACGAUGCGGUAUCUAUUUACACUUUUCCGCAGACGACUCGCCCGA 2991_H04
TCTTTATTCTCACTTTCCCG GGGAGGACGAUGCGGUCUUUAUUCUCACUUUCCCGCAGACGACUCGCCCGA 2992„H04
SACTTTTATACTTTTTGCCA GGGAGGACGAUGCGGUACUUUUAUACUUUU UGCCACAGACGACUCGCCCGA 2993„H04
ATCTTTTTTGTTCTTGTCTG GGGAGGACGAUGCGGAUCUUUUU UGUUCUUGUCUGCAGACGACUCGCCCGA 2994_H04
TACTT lTTTTTCCACTCCG GGGAGGACGAUGCGGUACUUU UUUUUUCCACUCCGCAGACGACUCGCCCGA 2999_.H04
GTTCTTTTCTTCCGCTACCG GGGAGGACGAUGCGGGUUCUU UUCUUCCGCUACCGCAGACGACUCGCCCGA 300Q_H04
ATCTTCTTTGTTTTTTACCC GGGAGGACGAUGCGGAUCUUCUUUGUUUUUUACCCCAGACGACUCGCCCGA 3Q01J-I04
TTTTTTTGTCTATCTTTTCCG GGGAGGACGAUGCGGUUUUUUUGUCUAUCUUUUCCGCAGACGACUCGCCCGA 3002_H04
TTTATTTACTTTGTTTCCCC GGGAGGACGAUGCGGUUUAUUUACUUUG UUUCCCCCAGACGACUCGCCCGA 3G03_H04
TTTCACTCTCTTTTTCGCGT GGGAGGACGAUGCGGUUUCACUCUCUUUUUCGCG UCAGACGACUCGCCCGA 3004„H04
TTTCTTTCTATTCTTTCCG GGGAGGACGAUGCGGUUUCUU UCUAUUCUU UCCGCAGACGACUCGCCCGA 3005_H04
TTATTTTATCACTT'ITGCG C GGGAGGACGAUGCGGUUAUUUUAUCACU UUUGCGCCAGACGACUCGCCCGA 3009 ΗΌ
# in H4
HTS read variable region SNA sequences selection selection Selected sequenc
TCATTTG CTCATTTCCTCCG GGGAGGACGAUGCGGUCAUU UGCUCAUUUCCUCCGCAGACGACUCGCCCGA 3010 H04
G GTTACTTTATCTTTTG CCG GGGAGGACGAUGCGGGGU UACUUUAUCU UUUGCCGCAGACGACUCGCCCGA 3011_H04
ACTATTGTTTTCTC1TTCCC GGGAGGACGAUGCGGACUAU UGUUUUCUCUUUCCCCAGACGACUCGCCCGA 3012_H04
TCTTTGAATCTTCTATTCCG GGGAGGACGAUGCGGUCUU UCAAUCUUCUAUUCCGCAGACGACUCGCCCGA 3013_H04
GGGAGGACGAUGCGGACUUCUUUCACUUCUUUCUGCAGACGACUCGCCCGA 3014__H04
TTTATTT7TCTTGCCCCCCG GGGAGGAC6AUGCGGUUUAUU UUUCUUGCCCCCCGCAGACGACUCGCCCGA 3015„H04
TCTCTTG1 ! I Α i I I I CtCC GGGAGGACGAUGCGGUCUCU UGU UUAUUUU UCCCCCAGACGACUCGCCCGA 3016_H04
TCTTAACTTCTTTTCCTCTG GGGAGGACGAUGCGGUCUUAACUUCUUUUCCUCUGCAGACGACUCGCCCGA 3017_H04
TCTTTTCAACCTTTTACCCG GGGAGGACGAUGCGGUCUUUUCAACCUUUUACCCGCAGACGACUCGCCCGA 3018_H04
TTTTCTATTATCTTCTTGTG GGGAGGACGAUGCGGUUUUCUAUUAUCUUCU UGUGCAGACGACUCGCCCGA 3019_H04
TTTTTG ATTCTTG TTTCCG GGGAGGACGAUGCGGUUUU UGAUUCUUGUU UCCGCAGACGACUCGCCCGA 3020_H04
TCTTTATTG TTTTTTTAC CC GGGAGGACGAUGCGGUCUU UAUUGUUUUUU UACCCCAGACGACUCGCCCGA 3021_HG4
ττττςτττΑτσττοττΘπο GGGAGGACGAUGCGGUU UUCUUUAUGUUGUUGU UGCAGACGACUCGCCCGA 3022„HO4
CCT7TATTTTTATCTTACC6 GGGAGGACGAUGCG6CCUUUAUUUUUAUCUUACCGCAGACGACUCGCCCGA 3023_ H04
TTGTTTTTCTATTGTTTGCG GGGAGGACGAUGCGGUUGUUU UUCUAUUGUUUGCGCAGACGACUCGCCCGA 3024_H04
TTTCTCAG CTTTTTTTTTCCG GGGAGGACGAUGCGG UU UCUCAGCU UUUUUUUUCCGCAGAGGACUCGCCCGA 3025_H04
CTTTTTATCTTACTTTTCCC GGGAGGACGAUGCGGCUUUUUAUCUUACUUUUCCCCAGACGACUCGCCCGA 3026_H04
TCCTGTCGGCTGTTGGTCCC GGGAGGACGAUGCGGUCCUGUCGGCUGUUGGUCCCCAGACGACUCGCCCGA 3028_H04
GTCCTGTCGTCTGTTTCGTCCC GGGAGGACGAUGCGGGUCCUGUCG UCUG UUUCGUCCCCAGACGACUCGCCCGA 3029__H04
TCTTGTCGTCTTTGCGTCCC GGGAGGACGAUGCGGUCUUG UCGUCUUUGCGUCCCCAGACGACUCGCCCGA 3030_H04
TCCTGTCGTCTTTGCGCCCC GGGAGGACGAU6CGGUCCUGUCGUCUUUGCGCCCCCAGACGACUCGCCCGA 3032._ H04
TCCTG TCG TCTGTTCGG TTC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCGGUUCCAGACGACUCGCCCGA 3034 H04
CGATTCCTCTGTTTCGTCCC GGGAGGACGAUGCGGCGAUUCCUCUGUUUCG UCCCCAGACGACUCGCCCGA 3035_H04
TGAGTCGCTCCCTTCGTCCC GGGAGGACGAUGCGGUGAGUCGCUCCCUUCGUCCCCAGACGACUCGCCCGA 3036 H04
CGCTTTTCTTTTTCTCG CCC GGGAGGACGAUGCGGCGCUUUUCUU UUUCUCGCCCCAGACGACUCGCCCGA 3038 H04
TTTGGGATTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGGAUUAGG UGUUCCGCAGACGACUCGCCCGA 3039 H04
U in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
TT7GGGAGTAGGTGTTCCG GGGAGGACGAUGCGGU UUGGGAGUAGGUGUUCCGCAGACGACUCGCCCGA 3041 H04
TTTCTTTAATTTCCCCGCCG GGGAGGACGAUGCGGU UUCUUUAAUUUCCCCGCCGCAGACGACUCGCCCGA 3042_ HQ4
TTTGGTGTAGGGGGGTTCCG GGGAGGACGAUGCGGU UUGGUGUAGGGGGGUUCCGCAGACGACUCGCCCGA 3043_HQ4
TTTATTCCACmCTCTCCG GGGAGGACGAUGCGGUUUAUUCCACUUUCUCUCCGCAGACGACUCGCCCGA 3G44„H04
TGmATTTTGTTcrrGccG GGGAGGACGAUGCGGUGU UUAUUUUGUUCUUGCCGCAGACGACUCGCCCGA 3045_H04
GTTCTTATTTCCTCTGTC C G G6GAGGACGAUGCGGGUUCUUAUUUCCUCUG UCCGCAGACGACUCGCCCGA 3046_HD4
AATCTTTGTATTTTTCGCCG GGGAGGACGAUGCGGAAUCU UUGUAUUUUUCGCCGCAGACGACUCGCCCGA 3047_H04
TTATTTTATCTCTG GCTCCG GGGAGGACGAUGCGGUUAUUUUAUCUCUGGCUCCGCAGACGACUCGCCCGA 3048_HQ4
CCGTTTC ATCTTTTTTACC G GGGAGGACGAUGCGGCCGUU UCAUCUUUU UUACCGCAGACGACUCGCCCGA 3049_H04
TCTCG CTTTCTTTTACTCCG GGGAGGACGAUGCGGUCUCGCUUUCUUUUACUCCGCAGACGACUCGCCCGA 3Q5D__HC4
TCTTTCATTTCTTTC CCCGC GGGAGGACGAUGCGGUCUUUCAUUUCUUUCCCCGCCAGACGACUCGCCCGA 3053„H04
CTTTTATTGTTTCCTTCCG GGGAGGACGAUGCGGCU UUUAUUGUUUCCUUCCGCAGACGACUCGCCCGA 3055_H04
TCATTTTGTTATCTTTTGTG GGGAGGACGAUGCGGUCAUUUUGUUAUCUUUUGUGCAGACGACUCGCCCGA 3056..H04
TCGCTTTCATTCATTTTCCG GGGAGGACGAUGCGGUCGCUUUCAUUCAUUUUCCGCAGACGACUCGCCCGA 30S7_H04
GCTCTTTCACCTTTCTTCCG GGGAGGACGAUGCGGGCUCUUUCACCUUUCUUCCGCAGACGACUCGCCCGA 3058_H04
ATATATCATTCTTT CACCC GGGAGGACGAUGCGGAUAUAUCAUUCUUU UCACCCCAGACGACUCGCCCGA 3059_H04
CCCTTTT ATC CTG TTTT CCG GGGAGGACGAUGCGGCCCUUUUAUCCUGUUUUCCGCAGACGACUCGCCCGA 3G60._HG4
AACACTTTCTTTCTCTACCG GGGAGGACGAUGCGGAACACU UUCUUUCUCUACCGCAGACGACUCGCCCGA 3061.. H04
ATAACTTTTTACTCTCACCG GGGAGGACGAUGCGGAUAACUUUUUACUCUCACCGCAGACGACUCGCCCGA 3062JH04
TTTTCTTTC A A CCTTTG CCG GGGAGGACGAUGCGGUU LIUCUUUCAACCUUUGCCGCAGACGACUCGCCCGA 3O63_.H04
TCTTTTTGTCACTTCTCCGC GGGAGGACGAUGCGGUCUUUUUGUCACUUCUCCGCCAGACGACUCGCCCGA 3064_HQ4
CTTATCTTC A ΤΤΠΤΤΑ CCG GGGAGGACGAUGCGGCU UAUCUUCAUUUUUUACCGCAGACGACUCGCCCGA 3065 HQ 4
ACTCTGTTTTTT TCCTTCCG GGGAGGACGAUGCGGACUCUGUU UUUUUUCCUUCCGCAGACGACUCGCCCGA 3066_H04
GTTTTGTATCTrTGTTACCG GGGAGGACGAUGCGG6UUU UGUAUCUUUGU UACCGCAGACGACUCGCCCGA 3067 H04
TCTATTTTCTTTCTCTACCC GGGAGG/ CGAUGCGGUCUAUUUUCUUUCUCUACCCCAGACGACUCGCCCGA 306S_H04
TTTCCTTATATTCTTCTGTG GGGAGGACGAUGCGGUUUCCUUAUAUUCUUCUGUGCAGACGACUCGCCCGA 3069 H04
# in H4
HTS read variable region NA sequences 3 selection selection Selected sequenc GTTCTTTATTGTATTCCC CG GGGAGGACGAUGCGGGUUCUUUAU UCUAUUCCCCGCAGA GACUCGCCCGA 3070_H04
TGTT1TCTCCGTTCTTG CCG GGGAGGACGAUGCGGUGUUUUCUCCGUUCUUGCCGCAGACGACUCGCCCGA 3071 H04
ATTCCTTACTATCTCTTCCG GGGAGGACGAUGCGGAUUCCUUACUAUCUCUUCCGCAGACGACUCGCCCGA 3073_H04
TCATT'rCTCTCCTTTTCCG GGGAGGACGAUGCGGUCAU UUCUCUCCUUU UCCGCAGACGACUCGCCCGA 3D74_H04
ccTTGTC 1 1 rm rcTTCCG GGGAGGACGAUGCGGCCUUGUCUUUUUUUCUUCCGCAGACGACUCGCCCGA 3075_H04
TTTATTTCTTTCCACTTTCCG GGGAGGACGAUGCGGU UUAUUUCUUUCCACUULCCGCAGACGACUCGCCCGA 3076_H04
CCTTTTAGTTCCTTTTACCG GGGAGGACGAUGCGGCCUUUUAGUUCCUUUUACCGCAGACGACUCGCCCGA 3Q77_H04
ACTTTATTCTCTCACTTCCG G6GAGGACGAUGCG6ACUUUAUUCUCUCACUU-CCGCAGACGACUCGCCCGA 3078._H04
ATTACATTTTCATTTCACCC GGGAGGACGAUGCGGAUUACAUUUUCAUUUCACCCCAGACGACUCGCCCGA 3079_H04
TTTCTCAATATTTTCGCCCG GGGAGGACGAUGCGGUUUCUCAAUAUUUUCGCCCGCAGACGACUCGCCCGA 3080_H04
TTTCACTTTTcrrmcccA GGGAGGACGAUGCGGUUUCACUUUUCUUUUUCCCACAGACGACUCGCCCGA 3081_H04
ACATTCCTTACTTTTTTCCG GGGAGGACGAUGCGGACAUUCCUUACUUUUUUCCGCAGACGACUCGCCCGA 3082_ H04
ATTTTGTTTTCGTTTTTGCCA GGGAGGACGAUGCGGAUUUUGUUUUCGUUUUUGCCACAGACGACUCGCCCGA 3083J 04
TTTTAACTCTTTTCTCTCTG GGGAGGACGAUGCGGU UUUAACUCUUUUCUCUCUGCAGACGACUCGCCCGA 3084_H04
TATfCTTTTGTTTCTTTTCG GGGAGGACGAUGCGGUAUUCUUUUG UUUCUUUUCGCAGACGACUCGCCCGA 3085_H04
TTTATTCTTATTTTG CT CC A GGGAGGACGAUGCGGUUUAUUCUUAUUUUGCUCCACAGACGACUCGCCCGA 30S6_ H04
TTTCCATTCTTTCATATGCG GGGAGGACGAUGCGGUU UCCAUUCUUUCAiJAUCCGCAGACGACUCGCCCGA 3087_HG4
TATATTCTGTTTTCCTTCTG GGGAGGACGAUGCGGUAUAUUCUGUUUUCCUUCUGCAGACGACUCGCCCGA 3088_H04
CCTTTTTTATCCCCT TTCCG GGGAGGACGAUGCGGCCUUUUUUAUGCCCUUUCCGCAGACGACUCGCCCGA 3089_H04
CTTTTTTAGTCCTTTTG CCG GGGAGGACGAUGCGGCUUUUUUAGUCCUUUUGCCGCAGACGACUCGCCCGA 3090_H04
CATTTATCTCTCT7TTCCCG GGGAGGACGAUGCGGCAUUUAUCUCUCUUUUCCCGCAGACGACUCGCCCGA 3091_H04
ATTCTTAATTGTTTTTACCG GGGAGGACGAUGCGGAUUCUUAAUUGUUUUUACCGCA6AC6ACUCGCCCGA 3092JHQ4
GATCTTTTATTTTCACACCC GGGAGGACGAUGCGGGAUCU UUUAUU UUCACACCCCAGACGACUCGCCCGA 3093.. H04
CTTTCATTTTTTTTCTCTCCG GGGAGGACGAUGCGGCUUUCAUUUUUUUUCUCUCCGCAGACGACUCGCCCGA 3094 H04
TTCTTCTTACTTCCTTCCG GGGAGGACGAUGCGGUUCUUCUUACUUCCUUCCGCAGACGACUCGCC GA 3095 H04
CTTTTCTACTATTTACCCCG GGGAGGACGAUGCGGCUUUUCUACUAUU UACCCCGCAGACGACUCGCCCGA 3096 H04
# in H4
HTS read variable region RMA sequences # in H3 selection selection Selected sequenc TTCTCATTCGT7ACTTTCCG GGGAGGACGAUGCGGU UCUCAUUCGUUACUUUCGGCAGACGACUCGCCCGA 3097_ H04
CTTTTCTTTCTCTTTCCG GGGAGGACGAUGCGGCUUUUCUUUCUCUUUCCGCAGACGACUCGCCCGA 3098_H04
AGTTTGTCTTTGTTTTTGCC GGGAGGACGAUGCGGACUUUGUCUUUGUUUUUGCCCAGACGACUCGCCCGA 3099_H04
GTTTTTCAATTTTCATTCCG GGGAGGACGAUGCGGGUUUUUCAAUUUUCAUUCCGCAGACGACUCGCCCGA 3102JH04
TTTTTGTTTTATTGCCTGCG GGGAGGACGAUGCGGUUU UUG UUUUAUUGCCUGCGCAGACGACUCGCCCGA 3103J 04
TTTCACTTTTCTCTTCGCCA GGGAGGACGAUGCGGUUUCACUUUUCUCU UCGCCACAGACGACUCGCCCGA 3104_H04
CGTTTATATTTTCCTTCCCG GGGAGGACGAUGCGGCG UUUAUAUUUUCCUUCCCGCAGACGACUCGCCCGA 31G5J-I04
TC ATTTCTTAGTT CTTCCC G GGGAGGACGAUGCGGUCAUUUCUUAGUUCUUCCCGCAGACGACUCGCCCGA 3106_HG4
TTTTCTTTTAACACTTTCTG GGGAGGACGAUGCGGUUUUCUUUUAACACUUUCUGCAGACGACUCGCCCGA 3107_H04
ATTTATATCACTCTTTTGCC GGGAGGACGAUGCGGAUUUAUAUCACUCUUUUGCCCAGACGACUCGCCCGA 3108_H04
ATCTTCACAATTTTTCGCCG GGGAGGACGAUGCGGAUCIJUCACAAUUUUUCGCCGCAGACGACUCGCCCGA 3109_H04
TTTTCTATTTTTCCCCTATG GGGAGGACGAUGCGGUUUUCUAUUUUUCCCCUAUGCAGACGACUCGCCCGA 3Π0_Η04
•J\ TCCTTTGTTTCTTTACCCCG GGGAGGACGAUGCGG UCCUUUG UUUCUUUACCCCGCAGACGACUCGCCCGA 3111„H04
TTTTATTTCTATGTGTTCCC GGGAGGACGAUGCGGUUUUAUUUCUAUG UGUUCCCCAGACGACUCGCCCGA 3112_H04
TTTCTT.CCTG TTTTTCC CCG GGGAGGACGAUGCGGUUUCUUCCUGUUUU UCCCCGCAGACGACUCGCCCGA 3114_H04
TTTG CTCTTTTCTTATTC CC GGGAGGACGAUGCGGUUUGCUCUUUUCUUAU UCCCCAGACGACUCGCCCGA 311S_ H04
CTTACTGTTTTTTCTTTCCG GGGAGGACGAUGCGGCUUACUGUUU UUUCUUUCCGCAGACGACUCGCCCGA 3117_H04
TTTTTATTTTGTTACATGTG GGGAGGACGAUGCGGUUUU UAUUU UGUUACAUGUGCAGACGACUCGCCCGA 3119JH04
TCTTATTTTGTATTTTCCCG GGGAGGACGAUGCGGUCUUAUUUUGUAUUUUCCCGCAGACGACUCGCCCGA 3120_ H04
AATTATTTTCTTTCACACTG GGGAGGACGAUGCGGAAUUAUU U UCUUUCACACUGCAGACGACUC6CCCGA 3121_H04
TTTATTCCATTT ATTTTCCG GGGAGGACGAUGCGGUU UAU UCCAUUUAUUUUCCGCAGACGACUCGCCCGA 3122_H04
TTTATTTTTTTCCCGTTTGTG GGGAGGACGAUGCGGUUUAU UUUUUUCCCGUUUGUGCAGACGACUCGCCCGA 312'3_H04
TTTTTACTTG ATCCTTTCTG GGGAGGACGAUGCGGUUUU UACUUGAUCCUUUCUGCAGACGACUCGCCCGA 3124_H04
ATTTTCTTTTTTTTCTG CCG GGGAGGACGAUGCGGAUUUUCUUU UUUUUCUGCCGCAGACGACUCGCCCGA 3125_H04
ATATTCGTTGTTTCTTCCCG GGGAGGACGAUGCGGAUAUUCGUUGUUUCUUCCCGCAGACGACUCGCCCGA 3127_H04
TTTCTCAGCTTTTTTTCCG GGGAGGACGAUGCGGUUUCUCAGCUU UUUUUCCGCAGACGACUCGCCCGA 3128 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
TTaAATTTTATTCCCTCCG GGGAGGACGAUGCGGUUCUAAUUUUAUUCCCUCCGCAGACGACUCGCCCGA 3129 H04
TTATTTTTGTTTTTTTGCGTG GGGAGGACGAUGCGGUUAU UUUUGU UUUU UUGCGUGCAGACGACUCGCCCGA 3130_H04
CCTTTTTCTTTCTTTAC CCG GGGAGGACGAUGCGGCCUUUUUCUUUCUUUACCCGCAGACGACUCGCCCGA 3131_HG4
ATTCCACTTTTTTT'nTC ACCG GGGAGGACGAUGCGGAUUCCACU UUUUUU UUUCACCGCAGACGACUCGCCCGA 3132_ HQ4
GTTTTGTTTTTTTCCTTTCCG GGGAGGACGAUGCGGGUUUUG UUUUUUUCCUUUCCGCAGACGACUCGCCCGA 3134J-104
GTTTTTTTATCACTTTTCCC GGGAGGACGAUGCGGGUUUUU UUAUCACUUUUCCCCAGACGACUCGCCCGA 3135JH04
GATTTG ΓΓΤΤΤΤΰΤΤΠ TAG GGGAGGACGAUGCGGGAUUUGUUUUUUGUUUUGUACCAGACGACUCGCCCGA 3136_H04
r CTTATTTTCTTTTCTGTG GGGAGGACGAUGCGGUUCUiJAUUUUCUUUUCUGUGCAGACGACUCGCCCGA 3137„H04
ACTGTTCTCTATTTTTACCG GGGAGGACGAUGCGGACUGUUCUCUAUUU UUACCGCAGACGACUCGCCCGA 3138_H04
TATTnCTACCTTTTTTTCCG GGGAGGACGAUGCGGUAUU UUCUACCUUU UU UUCCGCAGACGACUCGCCCGA 3139..H04
ATTTTATTG TTTTTTACCG GGGAGGACGAUGCGGAUUU UAUUGUUUUUUACCGCAGACGACUCGCCCGA 3141_H04
TTCTTTATTTTTTCCTCCG GGGAGGACGAUGCGGUUCU UUAUUUUUUCCUCCGCAGACGACUCGCCCGA 3142_HG4
ATTCTCTTTC ! I H l iCACCG GGGA6GACGAUGCGGAUUCUCUUUCUUUUUUCACCGCAGACGACUCGCCCGA 3143_HQ4
TTTCATC i M l ! ! i TCCG GGGAGGACGAUGCGG UU UCAUCUUUU UUUUCCGCAGACGACUCGCCCGA 3145JH04
CT'OTTTTATG I ) I i 1 1 CCC GGGAGGACGAUGCGGCUCUUUUUAUGUUUUUUCCCCAGACGACUCGCCCGA 3146_HC4
TTTTCATTCi ί I I ! I TTCCCC GGGAGGACGAUGCGGUUUUCAU UCUUUUUUUUCCCCCAGACGACUCGCCCGA 3147 JHQ4
CTTCTTTTCCATTATTTCCG GGGAGGACGAUGCGGCU UCUUUUCCAUUAU UCCGCAGACGACUCGCCCGA 3148_H04
TGAGTCG'ITCCTTTCGTCCC GGGAGGACGAUGCGGUGAGUCGUUCCU UUCGUCCCCAGACGACUCGCCCGA 3149_HQ4
TGAGTCGTTTCCTTCGTCCC GGGAGGACGAUGCGGUGAGUCGUUUCCUUCGUCCCCAGACGACUCGCCCGA 3150_H04
TCCTG TCG TCCTTG CGTCCC GGGAGGACGAUGCGGUCCUG UCGUCCUUGCG UCCCCAGACGACUCGCCCGA 3152_H04
TTTGGGGTTTTCCCGTGCCA GGGAGGACGAUGCGGUU UGGGGUU UUCCCGUGCCACAGACGACUCGCCCGA 3153_H04
ATTCACTTCTTTCTTGCCG GGGAGGACGAUGCGGAUUCACUUCUUUCUUGCCGCAGACGACUCGCCCGA 3156_H04
TTTAATTCATTCCCGTTCCG GGGAGGACGAUGCGG UU UAAUUCAUUCCCGUUCCGCAGACGACUCGCCCGA 3159_H04
TTCCCTGTTATTTTCTCCG GGGAGGACGAUGCGG UUCCCUGUUAUUUUCUCC6CAGACGACUCGCCCGA 3160_H04
CTTTTCCnTTCCCfATCCG GGGAGGACGAUGCGGCUUUUCCUUU UCCGUAUCCGCAGACGACUCGCCCGA 3163_HQ4
G TCTCCTG G CTTATCGTCCC GGGAGGACGAUGCGGGUCUCCUGGCUUAUCGUCCCCAGACGACUCGCCCGA 3164 H04
U in H4
HTS read variable region RNA sequences :3 selection selection Selected sequenc ATTCTTGCCTTTTTT6CCG GGGAGGACGAUGCGGAUUCUUGCCUUUUUUGCCGCAGACGACUCGCCCGA 3165_H04
TTTCCTTGTCTCCATTTCCG GGGAGGACGAUGCGGUUUCCUUGUCUCCAUU UCCGCAGACGACUCGCCeGA 3168_H04
G TTTTTATG CTTCCTCTC C G GGGAGGACGAUGCGGGUU UUUAUGCUUCCUCUCCGCAGACGACUCGCCCGA 3169_H04
CTTTAITTTTGTTTTTGCCC GGGAGGACGAUGCGGCUUUAU UUUUGUUUUUGCCCCAGACGACUCGCCCGA 3170_HQ4
TCTTGTTTTTTTTCTTTGCCGC GGGAGGACGAUGCGGUCUUGUUUUUUUUCUUUGCCGCCAGACGACUCGCCCGA 3171_H04
GATTCTTGTTACTTTCACCC GGGAGGACGAUGCGGGAUUCUUGUUACUUUCACCCCAGACGACUCGCCCGA 3174_H04
TCTTTTCG AACTTGTTTCCG GGGAGGACGAUGCGGUCUU UUCGAACUUGUUUCCGCAGACGACUCGCCCGA 3175_H04
CTTTCTCTTCCGTTGTTCCG GGGAGGACGAUGCGGCUUUCUCUUCCGUUGU UCCGCAGACGACUCGCCCGA 3176..H04
TTTTATT'CCTTTCCGCTCCG GGGAGGACGAUGCGGU UUUAU UCCUUUCCGCUCCGCAGACGACUCGCCCGA 3180_H04
TCTTATTCGATTTCCCTC CG GGGAGGACGAUGCGGUCUUAUUCGAUUUCCCUCCGCAGACGACUCGCCCGA 3 81_H04
GTTTATTCCCTGTTGTTCCG GGGAGGACGAUGCGGGUUUAUUCCCUGUUGUUCCGCAGACGACUCGCCCGA 3182JH04
CCTTTTACCTCACTTATCCG GGGAGGACGAUGCGGCCUUUUACCUCACUUAUCCGCA6ACGACUCGCCCGA 3183_H04
TTTTTATACATGTTCCTCCG GGGAGGACGAUGCGGUUUUUAUACAUGUUCCUCCGCAGACGACUCGCCCGA 3184 H04
CTTTTATTCTCTCTTCCCCG GGGAGGACGAUGCGGCUUUUAUUCUCUCUUCCCCGCAGACGACUCGCCCGA 3185_H04
ACTTTGTTACCTCTTTACCG GGGAGGACGAUGCGGACUU UGUUACCUCUUUACCGCAGACGACUCGCCCGA 3186_H0
TTACT7TTCTAACTTCTCCG GGGAGGACGAUGCGGUUACUU'JUCUAACUUCUCCGCAGACGACUCGCCCGA 3187_H04
ATTCTCCTGCTTTTCTTCCG GGGAGGACGAUGCGGAU UCUCCUGCUUUUCUUCCGCAGACGACUCGCCCGA 3188_H04
ACTTTCATACT7TTTCACCG GGGAGGACGAUGCGGACUU UCAUACUUUU UCACCGCAGACGACUCGCCCGA 3189_H0
TATTCTTCTTTCGCTTTGTG GGGAGGACGAUGCGGUAUUCUUCUUUCGCUUUGUGCAGACGACUCGCCCGA 31S0_H04
CTTCTTGTGTTTTTTTTGCTG GGGAGGACGAUGCGGCU UCUUGUGUUUU UUUUGCUGCAGACGACUCGCCCGA 31 1_H04
TTTGAACTCTTTTTTTTTCCG GGGAGGACGAUGCGGU UUGAACUCUUUUUUUUUCCGCAGACGACUCGCCCGA 3192_H04
CCATGTTTTCCTTTTATCCG GGGAGGACGAUGCGGCCAUG UUUUCCU UUUAUCCGCAGACGACUCGCCCGA 3193 H04
AACTACi i I I I [ !CTCTCTCCG GGGAGGACGAUGCGGAACUACUUUUUUUCUCUCUCCGCAGACGACUCGCCCGA 3194 H04
TTTTTCACTTTTTTTCGCCCCG GGGAGGACGAUGCGGUUUU UCACUUUUUUUCGCCCCGCAGACGACUCGCCCGA 3195 H04
ATTTCTTTTCCTTTTTGCCG GGGAGGACGAUGCGGAUUUCUUUUCCUUU UUGCCGCAGACGACUCGCCCGA 3196 H04
TCTTTTTTTTTTGTTTCCCCCG GGGAGGACGAUGCGGUCUU UUUUU UUUGUU UCCCCCGCAGACGACUCGCCCGA 3197 H04
# in H4
HTS read variable region NA sequences 3 selection selection Selected sequenc GATTTTCTTGCCnTTACCG GGGAGGACGAUGCGGGAUU UUCUUGCCU UUUACCGCAGACGACUCGCCCGA 3198_H04
TTTTTCCTATCTCCTTGCCG GGGAGGACGAUGCGGUUU UUCCUAUCUCCUUGCCGCAGACGACUCGCCCGA 3199 HQ4
TTT6GTAGTAGGGTGTTCCG GGGAGGACGAUGCGG UUUGGUAGUAGGGUGUUCCGCAGACGAC iCGCCCGA 3204_H04
GTTTCCTCTTCTTTTTCCCC GGGAGGACGAUGCGGG UUUCCUCUUCUUUUUCCCCCAGACGACUCGCCCGA 3205_H04
GATATTTACTTTTTTTT6GCC GGGAGGACGAUGCGGGAUAUU UACUUUUUUUUGGCCCAGACGACUCGCCCGA 3206_ HQ4
CTCATTTACACTTATTTCCG GGGAGGACGAUGCGGCUCAUUUACACUUAU UUCCGCAGACGACUCGCCCGA 3207_H04
CTTGTGTTTTTTTTTGCC GGGAGGACGAUGCGGCUUGUGUUUUUUUUUGCCCAGACGACUCGCCCGA 3208_H04
TTTTATTCTTTTCCGTTCCG GGGAGGACGAUGCGGU UUUAU UCUUUUCCGU LiCCGCAGACGACUCGCCCGA 3209„H04
TTCTTTGTTTATTCCCCCTG GGGAGGACGAUGCGGU UCUUUGUUUAUUCCCGCUGCAGACGACUCGCCCGA 3211_H04
CTTTTCTTTTTCCTCTTCTG GGGAGGACGAUGCGGCU UUUCUUU UUCCUCUUCUGCAGACGACUCGCCCGA 3212_HQ4
TTTTTTTCTACTTTTCACCG GGGAGGACGAUGCGGUUUUUUUCUACUUUUCACCGCAGACGACUCGCCCGA 3213_H04
TTTCTTTATTGTTTTTGTGC GGGAGGACGAUGCGGUUUCUU UAUUGUUUU UGUGCCAGACGACUCGCCCGA 3214_H04
TTTTTTTTTCT6TTTTCCCCG GGGAGGACGAUGCGGUUU UUUIjU !JCUGUUUUCCCCGCAGACGACUCGCCCGA 3215_H04
ACTTTCTCTTTTGTATTCCG GGGAGGACGAUGCGGACUUUCUCUUUUGUAUUCCGCAGACGACUCGCCCGA 3216_HQ4
TATTTTTTCAGGTTTCCCCG GGGAGGACGAUGCGGUAUUUUUUCAGGU UUCCCCGCAGACGACUCGCCCGA 3217_H04
TTCACTTrTTCTTTTTTGCG GGGAGGACGAUGCGGUUCACUUUUUCUUUUUUGCGCAGACGACUCGCCCGA 3218_H04
ATTGCGTACTTTTTTTTTCCG GGGAGGACGAUGCGGAU UGCGUACUUUUUUUUUGCGCAGACGACUCGCCCGA 3219JHG4
ATTTGCTTTTTTTTTGATTCCG GGGAGGACGAUGCGGAUUUGCU UUUUUUUUGAU JCCGCAGACGACUCGCCCGA 322Q_H04
GTTTCTACGACTTTTTTCC6 GGGAGGACGAUGCGGG UUUCUACGACUUUUU UCCGCAGACGACUCGCCCGA 3221 _HQ4
TCACTTTTTTTTCTACCCCG G6GAGGACGAUGCGGUCACUUUUUUUUCUACCCCGCAGACGACUCGCCCGA 3223_H04
ATTCTTCTCTACTTACACCG GGGAGGACGAUGCGGAUUCUUCUCUACUUACACCGCAGACGACUCGCCCGA 3224_H04
TATCTTTTAACTTTTG CCC G GGGAGGACGAUGCGGUAUCU UU UAACUUU UGCCCGCAGACGACUCGCCCGA 3225 H04
TGTTTTATTTGTTTTTCCC GGGAGGACGAUGCGGUG UUUUAUUUGUUUUUCCCCAGACGACUCGCCCGA 3226_H04
TATCTTGCTTCTCTTTTGCC GGGAGGACGAUGCGGUAUCU UGCUUCUCUUUUGCCCAGACGACUCGCCCGA 3227 H04
ACTTTACTTTACfCTTTCCC GGGAGGACGAUGCGGACUUUACUUUACUCU UUCCCCAGACGACUeGCCCGA 3228 H04
GTTCCATTTTTTTTTCCACCG GGGAGGACGAUGCGGGU UCCAUUUU U UUUUCCACCGCAGACGACUCGCCCGA 3229 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
ACTGCTTTTACTTTTTTCCC GGGAGGACGAUGCGGACUGCUUUUACUUUUUUCCCCAGACGACUCGCCCGA 3230 H04
ATCTTTTC G CTTTl ACCG GGGAGGACGAUGCGGAUCU UUUCGCUUUUUUUACCGCAGACGACUCGCCCGA 3232__H04
TACT 1 1 I I i ! ICO'CCACTCCG GGGAGGACGAUGCGGUACUUUUU UUUUUUCCACUCCGCAGACGACUCGCCCGA 3233JH04
ATriTCATTTTTTTTTCCG CCG GGGAGGACGAUGCGGAUUUUCAUU UUUUUU UCCGCCGCAGACGACUCGCCCGA 3234JHG4
ATTiTGC 11 I I T I l ACTACCG GGGAGGACGAUGCGGAUUUUGCUUUUUUUACUACCGCAGACGACUCGCCCGA 3235...H04
CTTTTTACTACTTTTCTCCG GGGAGGACGAUGCGGCU UUUUACUACUUUUCUCCGCAGACGACUCGCCCGA 3236_H04
TCTATGTTTATTTTGTCTCC6 GGGAGGACGAUGCGGUCUAUGUUUAUUUUG UCUCCGCAGACGACUCGCCCGA 3237_H04
ATTTTTTCCTGTTTTTTGCCG GGGAGGACGAUGCGGAUUU UUUCCUG'JUUUUUGCCGCAGACGACUCGCCCGA 3238_H04
TTCTTTATTACTATCTTCCG GGGAGGACGAUGCGGUUCUUUAUUACUAUCUUCCGCAGACGACUCGCCCGA 3240_H04
TTACCTm rrcTTTTCCCG GGGAGGACGAUGCGGUUACCUUUUUCUUUUCCCGCAGACGACUCGCCCGA 3241_ H0
TTTAT TTGTTTGTCGG GGGAGGACGAUGCGGUUUUUAUUUUUGUUUGUCGGCAGACGACUCGCCCGA 3242_H04
ATTTTTCTTC ATCTTTAC CG GGGAGGACGAUGCGGAUUUUUCUUCAUCUUUACCGCAGACGACUCGCCCGA 3243_H04
TACTTTGTTCTTCTTCTCTG GGGACGACGAUGCGGUACUUUG UUCUUCUUCUCLGCAGACGACiJ GCrCGA 3244_ HG4
TCTTTTTCTACCTTTTTTCCG GGGAGGACGAUGCGGUCUUUUUCUACCUUUUUUCCGCAGACGACUCGCCCGA 3245_H04
CGTTTTTCACTTACTTTCCG GGGAGGACGAUGCGGCGUU U UUCACUUACUUUCCGCAGACGACUCGCCCGA 3246.. H04
CTTTACTTATTTCATTTCTG GGGAGGACGAUGCGGCUUUACUUAUUUCAUUUCUGCAGACGACUCGCCCGA 3247JH04
CTTTTTiTTTCCTTTTTTTCCG GGGAGGACGAUGCGGCUUUU UUUUUCCUUUUUUUCCGCAGACGACUCGCCCGA 3248_H04
CATTTA ! I 'l l ί ITCCTTGGC GGGAGGACGAUGCGGCAUUUAUUUUUUUCCUUGGCCAGACGACUCGCCCGA 3249_H04
TTTCTTTTTCACTTCCCTATG GGGAGGACGAUGCGGUU UCUUUUUCACUUCCCUAUGCAGACGACUCGCCCGA 325Q_H04
TCATTTCTTTCTTATTCCCC GGGAGGACGAUGCGGUCAUUUCUUUCUUAUUCCCCCAGACGACUCGCCCGA 3251_H04
TTTGGTTTTAATTTTTTCCCG GGGAGGACGAUGCGGUU UGGUUUUAAUUUU UUCCCGCAGACGACUCGCGCGA 3252JHQ4
C ATTTC Π" S"l "I I CTTTTCC G GGGAGGACGAUGCGGCAUUUCUU UUUUCUUU UCCGCAGACGACUCGCCCGA 3253 H04
TCTCTTTG TACTTACTTC CG GGGAGGACGAUGCGG UCUCUUUGUACUUACUUCCGCAGACGACUCGCCCGA 3254,„H04
TACTTCTTTTTTTI' ί CTCTCC6 GGGAGGACGAUGCGGUACUUCUUUUUUUUUCUCUCCGCAGACGACUCGCCCGA 3255 H04
TATTCTTTATTA TCTTGTC GGGAGGACGAUGCGGUAUUCUUUAU UAUUCUUGUCCAGACGACUCGCCCGA 3257_H04
ATCTACACTT I I I I I t I I CCCG GGGAGGACGAUGCGGAUCUACACUUUUUUUUU UCCCGCAGACGACUCGCCCGA n H0
# in H4
HTS read variable region RNA sequences lection selection Selected sequenc TTTTATCTTTCTTTTTCCC GGGAGGACGAUGCGGUUUUAUCUUUCUUUUUCCCCAGACGACUCGCCCGA 3259_HG4
TCTTTTAACTTTTTCTCC6 GGGAGGACGAUGCGGUCUUUUAACUUUU UCUCCGCAGACGACUCGCCCGA 3260 H04
TCCTTGTGTCTTG GTCCC GGGAGGACGAUGCGGUCCUUGUGUCUUGCGUCCCCAGACGACUCGCCCGA 3261_H04
TTTGGGGTTrTTCCGTGCCC GGGAGGACGAUGCGGUUUGGGGUUUUUCCGUGCCCCAGACGACUCGCCCGA 3262_H04
TCCTGTCGTCTCTGCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUCUGCGUCCCCAGACGACUCGCCCGA 3263„H04
TTCCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGU UCCUGUCGUCUGUUCG UCCCCAGACGACUCGCCCGA 3266J 04
TCATTGTTTTTG TG TG G CCC GGGAGGACGAUGCGGUCAUUGUUUUUGUGUGGCCCCAGACGACUCGCCCGA 3267_H04
TTCG AAGGGTGTTGG GTCCG GGGAGGACGAUGCGGU UC6AAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 3268_ H04
TTTAGCTTTTTTTTCCCTCCG GGGAGGACGAUGCGGU UUAGCUUUUUUUUCCCUCCGCAGACGACUCGCCCGA 3269_H04
TCACTTTGCCTTTCTTTCCG GGGAGGACGAUGCGGUCACUUUGCCUUUCUUUCCGCAGACGACUCGCCCGA 3270_H04
CTTCTTGCTTTTTTCCCCCG GGGAGGACGAUGCGGCUUCUUGCUUUUUUCCCCCGCAGACGACUCGCCCGA 327i _HG4
TTTCACTCTTTTTTTTCCCCG C GGGAGGACGAUGCGGUUUCACUCUUUUUUUUCCCCGCCAGACGACUCGCCCGA 3272J Q4
TCTGTATTCACTTTTTTCCC GGGAGGACGAUGCGGUCUG UAUUCACUUU UUUCCCCAGACGACUCGCCCGA 3273_H04
TTATTCTCATTTATCCTCCG GGGAGGACGAUGCGGUUAUUCUCAUU UAUCCUCCGCAGACGACUCGCCCGA 3274_ H04
G ACTTT ACCTTCTT ATTC CC GGGAGGACGAUGCGGGACUUUACCUUCUUAUUCCCCAGACGACUCGCCCGA 3275_H04
TTCCATTCTTTCTTATTCCC GGGAGGACGAUGCGGU UCCAUUCUUUCUUAUUCCCCAGACGACUCGCCCGA 3276JH04
ATTCTTrrCTTTTTCA CCG GGGAGGACGAUGCGGAU UCUUUUCUUUUUCACCGCAGACGACUCGCCCGA 3278_HQ4
GTCArrGCTATTTTTTTCCG GGGAGGACGAUGCGGG UWUUGCUAUUUUU UUCCGCAGACGACUCGCCCGA 3280_H04
TTTTCTTGCCCT ! TT I H TCCG GGGAGGACGAUGCGGU UUUCUUGCCCUUUUUUU UCCGCAGACGACUCGCCCGA 3281_ H04
ATTCTTCTTTC ACTTACCG C GGGAGGACGAUGCGGAUUCUUCUUUCACUUACC6CCAGACGACUCGCCCGA 32S2_H04
CTTTTCTCTTCTTGTTTACCT GGGAGGACGAUGCGGCU UUUCUCLIUCUUGUUUACCUCAGACGACUCGCCCGA 3283_H04
GG TTTTC AATCTTTTCCC CC GGGAGGACGAUGCGGGG UUUUCAAUCUUUUCCCCCCAGACGACUCGCCCGA 3284 H04
AATACTTCTTATTCTCTCCG GGGAGGACGAUGCGGAAUACUUCUUAUUCUCUCCGCAGACGACUCGCCCGA 3285_H04
ATCTTTTCG CTTTTTTG CCG GGGAGGACGAUGCGGAUCUU UUCGCUUUUUUGCCGCAGACGACUCGCCCGA 3286_ H04
CTT CTCTAT AACTCTCTCCG GGGAGGACGAUGCGGCUUCUCUAUAACUCUCUCCGCAGACGACUCGCCCGA 3289JH04
TTFATTTCTTTTCACGTTCCG GGGAGGACGAUGCGGUU UAUUUCUUUUCACGUUCCGCAGACGACUCGCCCGA 3290 H04
# in H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc ACTT ATACTTCTCTTCT C CG GGGAGGACGAUGCGGACUUAUACUUCUCUUCUC GCAGACGACUrGCCCGA 3292. _H04
GTTTTTTCCCACTGTTCCCG GGGAGGACGAUGCGGGUU UUUUCCCACUG UUCCCGCAGACGACUCGCCCGA 3293. H04
CTCTTTTATACTTATCTCCG GGGAGGACGAUGCGGCUCUUUUAUACUUAUCUCCGCAGACGACUGGCCCGA 3294_ _H04
rrCTTCACTCACTTTTCCCG GGGAGGACGAUGCGGUUCU UCACUCACUUUUCCCGCAGACGACUCGCCCGA 3295. „HG4
TTTTTCTAAATTTG CTTCCG GGGAGGACGAUGCGGU UUUUCUAAAUUUGCUUCCGCAGACGACUCGCCCGA 3296. _HQ4
ATCATCACTTTCTTTTTACCG GGGAGGACGAUGCGGAUCAUCACUUUCU UUUUACCGCAGACGACUCGCCCGA 3297_ .H04
CTCACTTTCATTCCAmCG GGGAGGACGAUGCGGCUCACUUUCAUUCCAUUCCGCAGACGACUCGCCCGA 3298. .H04
TTTCTTTTCTC GCTTTTCCG GGGAGGACGAUGCGGU UUCUUUUCUCGCUUUUCCGCAGACGAeUCGCCCGA 3299. .H04
ATmcTTCTcrcTTCTccG GGGAGGACGAUGCGGAUUU UCUUCUCUCUUCUCCGCAGACGACUCGCCCGA 3-300. .H04
CA TTTACTATTTCTCTCCG GGGAGGACGAUGCGGCAUUUUACUAUUUCUCUCGGCAGACGACUCGCCCGA 3301. _H04
GTCTTGTCTATTTTTTCCCCG GGGAGGACGAUGCGGGUCUUGUCUAUUUUUUCCCCGCAGACGACUCGCCCGA 3302. ,H04
TCTTTATTTACG G CTTTCCG GGGAGGACGAUGCGGUCUU UAUUUACGGCUUUCCGCAGACGACUCGCCCGA 3303. H04
•J\ TGGTTGGCGTTATGGAGGTG GGGAGGACGAUGCGGUGGUUGGCGUUAUGGAGG JGCAGACGACUCGCCCGA
oe 3304. .H04
CTTTTTTTTCATGCTTTCCG GGGAGGACGAUGCGGCUUU UUUUUCAUGCUUUCCGCAGACGACUCGCCCGA 3305. _MQ4
ATTTTCTCT T TTTTTTG C CG GGGAGGACGAUGCGGAUUUUCUCUUUUUUUUGCCGCAGACGACUCGCCCGA 3306. _HQ4
TCACTTCTTTCTATTCCCCG GGGAGGACGAUGCGGUCACU UGUUUCUAUUCCCCGCAGACGACUCGCCCGA 3307. ,H0
A CTTTTCTTTCTTTGC CG GGGAGGACGAUGCGGAUCUUUUCUUUCUUUGCCGCAGACGACUCGCCCGA 3308. JH 04
TTTCTTGATCCTTTTTTTCCG GGGAGGACGAUGCGGUUUCUUGAUCCUUUUU UUCCGCAGACGACUCGCCCGA 3309. _H04
CT ITTATGTTTCCCTTTCCG GGGAGGACGAUGCGGCU UUUAUGUUUCCCUUUCCGCAGACGACUCGCCCGA 3310. .H04
CCTTATTTATTTTCTTACCG GGGAGGACGAUGCGGCCUUAUU UAUUUUCUUACCGCAGACGACUCGCCCGA 3311. .H04
CTTCTTTTCTTTCTCTTGTG GGGAGGACGAUGCGGCU UCUUUUCUUUCUCUUGUGCAGACGACUCGCCCGA 3312. .H04
TTTCTTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUCUUUUCCUUUGUCCGCAGACGACUCGCCCGA 3313. .H04
ATTCCTTTTCTTTTITCTCCG GG6A6GACGAUGCGGAUUCCUU UUCUUUUUUCUCCGCAGACGACUCGCCC6A 3314. .H04
TTTAmCACACTCTITGCC GGGAGGACGAUGCGGUU UAUUUCACACUCUUUGCCCAGACGACUCGCCCGA 3315. _H04
CTTTCTATATATTCCTTCCG GGGAGGAC6AUGCGGCUUUCUAUAUAUUCCUUCCGCAGACGACUC6CCCGA 3317. _H04
TCTTTTCTGTTTCTGTCCCG GGGAGGACGAUGCGGUCUU UUCUGUUUCUGUCCCGCAGACGACUCGCCCGA 3318 H04
# in H
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTATTATCTTTCTCTGCTG GGGAGGACGAUGCGGUUUAUUAUCUU UCUCUGCUGCAGACGACUCGCCCGA 3319. _H04
TTTTCTTTCTCTCATCTCCG GGGAGGACGAUGCGGU UUUCUUUCUCUCAUCUCCGCAGACGACUCGCCCGA 3320. _H04
ATTATTGCTTTTTTTTGCTG GGGAGGACGAUGCGGAUUAUUGCU UUUUUUUGCUGCAGACGACUCGCCCGA 3321. .H04
TTTATTCTTTTTTCTTTGCG GGGAGGACGAUGCGGUUUAUUCUUUUUUCUUUGCGCAGACGACUCGCCCGA 3322. _H04
AGTTTCTTTTCTTTCTTCCG GGGAGGACGAUGCGGAGUU UCUUUUCUUUCUUCCGCAGACGACUCGCCCGA 3323. _H04
ACCTTATAACTCTTCTTCCG GGGAGGACGAUGCGGACCUUAUAACUCUUCUUCCGCAGACGACUCGCCCGA 3324. .H04
TGTTCACG ί I M i l l i TCTG GGGAGGACGAUGCGGUGUUCACGUUUUUUUUUCUGCAGACGACUCGCCCGA 3325. .H04
GTCTATTATTATTTCTGC CG GGGAGGACGAUGCGGGUCUAUUAU UAUUUCUGCCGCAGACGACUCGCCCGA 3326. ,H04
TTCATACTTTCTTTCTTGCC GGGAGGACGAUGCGGUUCAUACUUUCUUUCU UGCCCAGACGACUCGCCCGA 3327. .H04
ATCTTTATCTTCTTCTCCCG GGGAGGACGAUGCGGAUCUUUAUCUUCUUCUCCCGCAGACGACUCGCCCGA 3328. H04
TTATTnTmTCTGTCCG GGGAGGACGAUGCGGU UAU UU UUUUUUCUGUCCGCAGACGACUCGCCCGA 3329. _H04
GACTCTTTATTTCTTTTCCC GGGAGGACGAUGCGGGACUCUU UAUUUCUUU UCCCCAGACGACUCGCCCGA 3330. _H04
CCTTTTTGTTACTTTATCCC GGGAGGACGAUGCGGCCUUUUUGUUACUUUAUCCCCAGACGACUCGCCCGA 3331. _H04
TTTTTCATAACTTTCCTCTG GGGAGGACGAUGCGGUU UUUCAUAACU UUCCUCUGCAGACGACUCGCCCGA 3332. .H04
ACACTTATCACTTTTTACCG GGGAGGACGAUGCGGACACUUAUCACUUUU UACCGCAGACGACUCGCCCGA 3333. .H04
CTAGGGTGAAGGGTGGATGC GGGAGGAC6AUGCGGCUAGGGUGAAGGGUGGAUGCCAGACGACUCGCCCGA 3334. .H04
TTTTACCTCGTTTTTCTGTG GGGAGGACGAUGCGG UUUUACCUCGUUUUUCUGUGCAGACGACUCGCCCGA 3335. _H04
G CTTTCGTTCTGC CTCCC GGGAGGACGAUGCGGGCUUUCG UUCUGCCUCCCCAGACGACUCGCCCGA 3336. _H04
CTTCATGTTTAGCTTTCGCC GGGAGGAC6AUGCGGCU UCAUG UUUAGCUUUCGCCCAGACGACUCGCCCGA 3337. .H04
ATCTTTCTTTCTTCTG CCG GGGAGGACGAUGCGGAUCUUUCUUUCUUCUGCCGCAGACGACUCGCCCGA 3338. .H04
ATTTCGCTAA1 TTTACCG GGGAGGACGAUGC6GAUUUCGCUAAUUUU UUUACCGCAGACGACUCGCCCGA 3339. .H04
GCCTTCT rcTTTCTCccG GGGAGGACGAUGCGGGCCUUCUUUUCUUUCUCCCGCAGACGACUCGCCCGA 3340. _H04
TTATTCTCTATTCTTTTG CC GGGAGGACGAUGCGGU UAUUCUCUAUUCUUUUGCCCAGACGACUCGCCCGA 3341. .H04
TCTTTCATTTTCTCTCTCTG GGGAGGACGAUGCGGUCUUUCAUUUUCUCUCUCUGCAGACGACUCGCCCGA 3342. .H04
TTTGTAATGTTTTTI TTTTTCCG GGGAGGACGAUGCGGUU UGUAAUGUUU UUU UU UUCCGCAGACGACUCGCCCGA 3343. ,H04
TCATTACTGTTT TTTATCCG GGGAGGACGAUGCGGUCAUUACUGUUUUU UU UAUCCGCAGACGACUCGCCCGA 3344. .H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TTTTTTCAC I I I il l CGCCCCG GGGAGGACGAUGCGGUUUUUUCACUUUUUUCGCCCCGCAGACGACUCGCCCGA 3345_H04
ATTTTTTTTGTCGCTTCCCG GGGAGGACGAUGCGGAUUU JUUU UGUCGCUUCCCGCAGACGACUCGCCCGA 3346_H04
ATTTCTTTTCCTTTACCG GGGAGGACGAUGCGGAUUUCU UUUCCUU UACCGCAGACGACUCGCCCGA 3347_H04
TACTCTTTTATTTCTTCCCG GGGAGGACGAUGCGGUACUCUU UUAUUUCUUCCCGCAGACGACUCGCCCGA 3348„H04
TTTCTTAC'(TTTTTTCATCCG GGGAGGACGAUGCGGU UUCUUACUUUUUUUCAUCCGCAGACGACUCGCCCGA 3349_H04
TTTTTCAATTATTTCTGTCCG GGGAGGACGAUGCGGU UUUUCAAU UAUUUCUGUCCGCAGACGACUCGCCCGA 3350_H04
TTTTTGTCATTTCTTGTTCCG GG6A6GACGAUGCGGU UUUUGUCAUUUCUUGUUCCGCAGACGACUCGCCCGA 3355_H04
TTTTAGTTATGTTTTGTTCC GGGAGGACGAUGCGGU UUUAGUUAUGUUUUGUUCCCAGACGACUCGCCCGA 3356_H04
TTTCTTTTCTACTCATTCTG GGGAGGACGAUGCGGUUUCUU UUCUACUCAUUCUGCAGACGACUCGCCCGA 3357_H04
GTTTTTTGTAACT'GCTTCCC GGGAGGACGAUGCGGGUUUUUUG UAACUGCU UCCCCAGACGACUCGCCCGA 33S8_H04
TTTTTTTT ATTG TTG TTTCTG GGGAGGACGAUGCGGUUUU JUUUAUUGUUGUUUCUGCAGACGACUCGCCCGA 33S9_H04
CTCTTATCGTTATTTTTCCG GGGAGGACGAUGCGGCUCUUAUCGUUAUUUUUCCGCAGACGACUCGCCCGA 3360_H04
TTCACATTATTTTTTTTTCCG GGGAGGACGAUGCGGUUCACAUUAUUUUUUU UUCCGCAGACGACUCGCCCGA
ο 3361_H04
CTCATTATCTATCTCTTCCG GGGAGGACGAUGCGGCUCAUUAUCUAUCUCUUCCGCAGACGACUCGCCCGA 3362JH04
TCTTTTTATCACTATTTCCC GGGAGGACGAUGCGGUCUU UUUAUCACUAUUUCCCCAGACGACUCGCCCGA 3363„H04
CCTTTCATTTTTTTTTTTG CCG GGGAGGACGAUGCGGCCUUUCAUUUUUUUUUUUGCCGCAGACGACUCGCCCGA 3364_H04
TATTGTTTTCTCGTTTTGTG GGGAGGACGAUGCGGUAUUGU UUUCUCG UUUUGUGCAGACGACUCGCCCGA 3365_H04
CATCTCTTTCATTTTATCCG GGGAGGACGAUGCGGCAUCUCUUUCAUUUUAUCCGCAGACGACUCGCCCGA 3366_H04
GTTCTTTCATG I ! i ! I l i CCC GGGAGGACGAUGCGGGUUCUUUCAUGUU UUUUUCCCCAGACGACUCGCCCGA 3367_H04
AATTTCTCGTTTTTTTTTGCTG GGGAGGACGAUGCGGAAUU UCUCGUUUUUUUUUGCUGCAGACGACUCGCC GA 3368_K04
GTTGCTTAGTTrmTCCGC GGGAGGACGAUGCGGGU UGCUUACUU UUUUUCCGCCAGACGACUCGCCCGA 3369_H04
TTACTCATTTTATTTCTCCG GGGAGGACGAUGCGGUUACUCAUUUUAUUUCUCCGCAGACGACUCGCCCGA 3370_.H04
TTTTTCCTTTTTTTATCCG GGGAGGACGAUGCGGUU UUUCCUUUUUUUAUCCGCAGACGACUCGCCCGA 3371_H04
CCATTTGTTTTTTTTTCTTCCG GGGAGGACGAUGCGGCCAUU UGUUU UUUUUUCL UCCGCAGACGACUCGCCCGA 3372_H04
TACTTTAATCTTTTTTTCCG GGGAGGACGAUGCGG UACUUUAAUCUUUUUU UCCGCAGACCACUCGCCCGA 3374_H04
TCGTTTATTTCTTTTTCCG GGGAGGACGAUGCGGUCGUUUAUUUCUUUUUCCGCAGACGACUCGCCCGA 3375 H04
# in m
HTS read variable region RNA sequences H3 selection selection Selected sequenc CTTTCTCTTTAATTCTTCCG GGGAGGACGAUGCGGCUUUCUCUUUAAUUCU UCCGCAGACGACUCGCCCGA 3376_ HG4
CCTTTTTCTTTCCTTTCCG GGGAGGACGAUGCGGCCUUUUUCU UUCCUUUCCGCAGACGACUCGCCCGA 3377 H04
TCCTTGTCGTTTTGCGTCCC GGGAGGACGAUGCGGUCCUUGUCGUUUUGCGUCCCCAGACGACUCGCCCGA 3379_H04
TCCTTTCGTTCTG CCCTCCG GGGAGGACGAUGCGGUCCUUUCGUUCUGCCCUCCGCAGACGACUCGCCCGA 3380_HG4
TCTTCTGGC1TATCGTCCC G GG AGG ACG AUG CGG U C U U CU G GC U U A U CG UC CCC AG AC G AC U CGCCCG A 3381 _HQ4
TCCTTTCGTTGCCCTCCC GGGAGGACGAUGCGGUCCUUUCGUUGCCCUCCCCAGACGACUCGCCCGA 33S2. H04
GCCCTTCGTTCTTCCCTCCC GGGAGGACGAUGCGGGCCCU UCGUUCUUCCCUCCCCAGACGACUCGCCCGA 3383_H04
TCCTCTCGTTCTGCCCTCCC GGGAGGACGAUGCGGUCCUCUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 3385„H04
ATAGTCTGGACTT6CCGCCC GGGAGGACGAUGCGGAUAGUCUGGACUUGCCGCCCCAGACGACUCGCCCGA 3386_H04
TCCTGGCTTGTCGTGTCCC GGGAGGACGAU6CGGUCCUGGCUUGUCGUGUCCCCAGACGACUCGCCCGA 3387„H04
TCTTTTG CCTTCTTTTCCG GGGAGGACGAUGCGGUCUUUUGCCUUCUUUUCCGCAGACGACUCGCCCGA 3389_H04
TTTCACTTTTATTTCTGCCG GGGAGGACGAUGCGGUUUCACUUU UAUUUCUGCCGCAGACGACUCGCCCGA 3390_H04
TTTGGGAGTAGGGTGTGCCG GGGA6GACGAUGCGGUUUGGGAG UAGGGUGUGCCGCAGACGACUCGCCC6A 3391_H04
TTTGGGGTTTTTCCC6TGCCG GGGAGGACGAUGCGGUUUGGGGUUUUUCCCGUGCCGCAGACGACUCGCCCGA 3393_ BQ4
ATTTG A AGG GTG ATG G GTCCG GGGAGGACGAUGCGGAUUUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 3 94_H04
TTTCCATTCAATCTTCTCCG GGGAGGACGAUGCGGUUUCCAUUCAAUCUUCUCCGCAGACGACUCGCCCGA 3395_H04
TTTTTGTCTACTTTTCGCCG GGGAGGACGAUGCGGUUUUUGUCUACUUUUCGCCGCAGACGACUCGCCCGA 3396_H04
C'fTTTCCTTACTATTTTGCC GGGAGGACGAUGCGGCUUUUCCUUACUAUUUUGCCCAGACGACUCGCCCGA 3397_H04
ATGCTTGGAGTAGGGAGGCG GGGAGGACGAUGCGGAUGCUUGGAGUAGGGAGGCGCAGACGACUCGCCCGA 339H _HQ4
TCACTTTTTTACTTCCTGCC G GGAGG ACG AU G CG GUCACU UUUUUACUUCCU GCCCAGACG ACU CG CCCGA 3399_H04
ATCTTTCTTATTTTTGCCG GGGAGGACGAUGCGGAUCUUUCUUAU UUUUGCCGCAGACGACUCGCCCGA 3400_H04
TTTGGGGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGGGUAGGGUGUUCCGCAGACGACUCGCCCGA 3401_H04
TCTTACTTTTTCACTCCG GGGAGGACGAUGCGGUCUUACUUUUUCACUCCGCAGACGACUCGCCCGA 3402_H04
TTGTTTTCGl I Π 1 1 I ! GCC GGGAGGACGAUGCGGU UGU UU UCGUUU UUUUUGCCCAGACGACUCGCCCGA 3405 H0
GTTTCGTTCTGCCCTCCC G GG AGG ACGAUGCG GG UU U CG U UC UGCCCUCCCCAG ACGACUCGCCCG A 3406 H04
CTTTCAC n "I I ! I I CTCTCCC GGGAGGACGAUGCGGCU UUCACUUUUUUUCUCUCCCCAGACGACUCGCCCGA 3407 H04
# in H4
HT5 read variable region RNA sequences selection selection Selected sequenc GTTGGCGTAGGGGGGTTCCG GGGAGGACGAUGCGGGUUGGCGUAGGGGGGUUCCGCAGACGACUCGCCCGA 3408_H04
TTTTACTCTTTTCCGCTCCG GGGAGGAGGAUGCGGUUUUACUCUUUUCCGCUCCGCAGACGACUCGCCCGA 3409„H04
TCTTTTTAACTTTTCTCCG GGGAGGACGAUGCGGUCUUUUUAACUUUUCUCCGCAGACGACUCGCCCGA 3410„H04
ATTTGCTTTTTTTTGGTTCCG GGGAGGACGAUGCGGAUUUGCU UUU UUUUGGUUCCGCAGACGACUCGCCCGA 34I1_HG4
CTTTATTCTTGTTGTCTCCG GGGAGGACGAUGC6GCU UUAUUCUUGUUGUCUCCGCA6AC6ACUCGCCCGA 3412_H04
CATTCATTTTCTTTCTACCG GGGAGGACGAUGCGGCAUUCAUUUUCUUUCUACCGCAGACGACUCGCCCGA 3413„H04
TTTCTTGTTTC CTTTCCG GGGAGGACGA-UGCG6UUUCUUGUU UCCUUUCCGCAGACGACUCGCCC6A 3 14_H04
TTATTTGTATGTTTTTTCCG GGGAGGACGAUGCGGUUAUUUGUAUGUUUUUUCCGCAGACGACUCGCCCGA 3415„HG4
ACTTCGC'iTTTATTTATCCC GGGAGGACGAUGCGGACUUCGCUUUUAUUUAUCCCCAGACGACUCGCCCGA 3416_H04
CTCTTATTCTCTCATTTCCG GGGAGGACGAUGCGGCUCUUAUUCUCUCAU UUCCGCAGACGACUCGCCCGA 3417_H04
ATTTAATGTTCTCTTTTCCG GGGAGGACGAUGCGGAU UUAAUGUUCUCUUU UCCGCAGACGACUCGCCCGA 3418_H04
TTTITTG TTCCCTTTTG CCCG GGGAGGACGAUGCGGUUUU UUGUUCCCUUUUGCCCGCAGACGACUCGCCCGA 3419_H04
TTTTGTGTTTTATGTATCCG GGGAGGACGAUGCGGUUUUGUGUUUUAUGUAUCCGCAGACGACUCGCCCGA 3420_H04
ATCATTTTTTCATCCCACCG GGGAGGACGAUGCGGAUCAU UUUUUCAUCCCACCGCAGACGACUCGCCCGA 3421_H04
AACTTTTCTTCTCTCGTCCG GGGAGGACGAUGCGGAACUU UUCUUCUCUCGUCCGCAGACGACUCGCCCGA 3422_H04
CTTGTGTTTTTTTTTTTTG CC GGGAGGACGAUGCGGCUUGUGUUU UUUUUU UUUGCCCAGACGACUCGCCCGA 342 _H04
ATTTTTTTGATCCTTCTCCG GGGAGGACGAUGCGGAUUUUUUUGAUCCUUCUCCGCAGACGACUCGCCCGA 3424_ H04
GATTTTTCTTTTTTTGTGCCG GGGAGGACGAUGCGGGAUUUUUCUUUUU UUGUGCCGCAGACGACUCGCCCGA 3425_H04
CTCTTTCTTCGCTTTTCCCG GGGAGGACGAUGCGGCUCUU UCUUCGCUUUUCCCGCAGACGACUCGCCCGA 3426_HG4
GCTCTTTTCTTTCTTCACCC GGGAGGACGAUGCGGGCUCUUUUCUUUCUUCACCCCAGACGACUCGCCCGA 3427_H04
ATCTTTTCG ΤΤΤΤΤΤΓ A C C G GGGAGGACGAUGCGGAUCUUUUCGUUUUUUUACCGCAGACGACUCGCCCGA 3428JHG4
TAACTATT rCTTTCTGCCC GGGAGGACGAUGCGGUAACUAUUUUCUUUCUGCCCCAGACGACUCGCCCGA 3429_H04
TTTTTGTCTTGCTTTTTCCG GGGAGGACGAUGCGGUUUU UGUCUUGCUUUUUCCGCAGACGACUCGCCCGA 3430_H04
TTTCTTTTCGTTACTTGCCG GGGAGGACGAUGCGGUUUCUUUUCGUUACUUGCTGCAGACGACUCGCCCGA 3431 H04
G CTCTATTCTTTTCCCACCG GGGAGGACGAUGCGGGCUCUAUUCUUUUCCCACCGCAGACGACUCGCCCGA 3432_HQ4
G C7TTTCTTCCTTTTTTTCCG GGGAGGACGAUGCGGGCUUUUCUUCCUUUUUUUCCGCAGACGACUCGCCCGA 3434 H04
tt in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc ACCTTTTGATTTTTTTGttC GGGA6GACGAUGCGGACCUU UUGAU UUU UU UGCCCCAGACGACUCGCCCGA 3435 H04
GCTTTCTTTACGCTTTTCCC GGGAGGACGAUGCGGGCUUUCUUUACGCUUU UCCCCAGACGACUCGCCCGA 3436_R04
ΑπΑΤΓΤ€ΤΤΤΓσΤΤΤ6€€σ GGGAGGACGAUGCGGAUUAUU UCUUUCG UUUGCCGCAGACGACUCGCCCGA 343?_HG4
CGTTTTCTACπCTTTCCG GGGAGGACGAUGCGGCGUUUUCUACUUCUUUCCGCAGACGACUCGCCCGA 3438_H04
CTTTCAC ί I 1 I I I I TC CCTCCG GGGAGGACGAUGCGGCUUUCACUUUUUUUUCCCUCCGCAGACGACUCGCCCGA 3439„H04
TTTTTCATTCCCTTTTTTTCCG GGGAGGACGAUGCGGUUUUUCAUUCCCUUUUUU UCCGCAGACGACUCGCCCGA 344i_HG4
TTTGGGGGGAGGGGGTTCCG GGGAGGACGAUGCGGUUUGGGGGGAGGGGGUUCCGCAGACGACUCGCCCGA 3442_H04
TTTCCTTTG CTTTCTCTCCG GGGAGGACGAUGCGGUUUCCUU UGCUUUCUCUCCGCAGACGACUCGCCCGA 3 43 _H 04
CAATT6TTTTTTTTTCACCGC GGGAGGACGAUGCGGCAAU UGUUU UUUUUUCACCGCCAGACGACUCGCCCGA 3445__HG4
TTCATTTTTTTATTCCCTGTG GGGAGGACGAUGCGGUUCAUUUUUUUAUUCCCUGUGCAGACGACUCGCCCGA 3446_H04
CATTCT7TGTTC7TCTACGC GGGAGGACGAUGCGGCAUUCUUUGUUCUUCUACGCCAGACGACUCGCCCGA 3447_H04
TTTG TTCTTTTCTTTTTCCCC GGGAGGACGAUGCGGUUUGUUCUUUUCUUUUUCCCCCAGACGACUCGCCCGA 3448_HG4
TTCTTATTTATGTCTTTCCG GGGAGGACGAUGCGGUUCUUAUUUAUGUCUUUCCGCAGACGACUCGCCCGA 3449 .H04
AATTCTATTTATTTTTGCCG GGGAGGACGAUGCGGAAUUCUAUUUAUUUUUGCCGCAGACGACUCGCCCGA 3 50_HG4
CCTTT ATTATTTTTT A C CCG GGGAGGACGAUGCGGCCUUUAUUAUU UUUUACCCGCAGACGACUCGCCCGA 3451_H04
ACTTCTTCATATTCTeACCG GGGAGGACGAUGCGGACUUCUUCA'JAUUCUCACCGCAGACGACUCGCCCGA 3452_BG
CTTTTTCTATTTAGTTTCCG GGGAGGACGAUGCGGCUUU UUCUAUUUAGUUUCCGCAGACGACUCGCCCGA 3453_H04
ACTTGTTGTTCTTTTTACCG G6GAGGACGAUGCGGACUUGUUGU UCUUUU UACCGCAGAC6ACUCGCCCGA 3454raHQ4
ACATTACTriTTTTTTTCCCG GGGAGGACGAUGCGGACAUUACUUU UUUUUUUCCCGCAGACGACUCGCCCGA 34E.5. HQ4
CTTTTTGT1 CTTTTACTG GGGAGGACGAUGCGGCUUU UUGUUUUCUUUUACUGCAGACGACUCGCCCGA 3456_H04
TTTTCGCT ATTTTTCACCG C GGGAGGACGAUGCGGUUU UCGCUAUUUUUCACCGCCAGACGACUCGCCCGA 3457_H04
TTTTC ACTTTCTTTTTG CTC GGGAGGACGAUGCGGUUUUCACUUUCUUUUUGCUCCAGACGACUCGCCCGA 3458_H04
TCTCATTTGCTTTTATCCCG GGGAGGACGAUGCGGUCUCAUUUGCUUUUAUCCCGCAGACGACUCGCCCGA 3459J-I04
ACTCTAGTTTATTTTCACCG GGGAGGACGAUGCGGACUCUAGUUUAUUUUCACCGCAGACGACUCGCCCGA 3460_H04
ACTTTTTTCGTTTTCCAACCG GGGAGGACGAUGCGGACUUUUUUCG UUUUCCAACCGCAGACGACUCGCCCGA 3461_H04
ACTTTTCTCGCACTTTACCG GGGAGGACGAUGCGGACUUUUCUCGCACUU UACCGCAGACGACUCGCCCGA 3462 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc AACCTTGTTTTTCTTTCACCG GGGAGGACGAUGCGGAACCU UGUUUUUCUUUCACCGCAGACGACUCGCCCGA 3463_H04
AATTCCTCATATTTTCTCCG GGGAGGACGAUGCGGAAUUCCUCAUAUUUUCUCCGCAGACGACUCGCCCGA 3464_H.04
GTTTCTATTTCTCTCCGCCG GGGAGGACGAUGCGGG UUUCUAUU UCUCUCCGCCGCAGACGACUCGCCCGA 3465_H04
ATACTTTCTTTTTACCACTG GGGAGGACGAUGCGGAUACUUUCUUUUUACCACUGCAGACGACUCGCCCGA 3466_H04
TCTTG TTACTTTTCTTACCG GGGAGGACGAUGCGGUCUUGUUACUUUUCUUACCGCAGACGACUCGCCCGA 3467_H04
TTTCCAATCCmrCCTTCCG GGGAGGACGAUGCGGU UUCCAAUCCUUUCCUUCCGCAGACGACUCGCCCGA 346S_H04
TTATTATTATCTTTCTGCCC GGGAGGACGAUGCGGU UAU UAUUAUCUU UCUGCCCCAGACGACUCGCCCGA 3469_H04
TTTCAAATTTTACTTTTCCG GGGAGGACGAUGCGGU UUCAAAUUUUACU UUUCCGCAGACGACUCGCCCGA 3470_H04
TCTCTTTrrcATTGrrcccG GGGAGGACGAUGC6GUCUCUUUUUCAUUGUUCCCGCAGACGACUCGCCCGA 347i_H04
TTACTTTTTCTTTTGGCGTC GGGAGGACGAUGCGGUUACU UUUUCUUU UGGCG UCCAGACGACUCGCCCGA 3472_H04
CC I t I 1 1 1 I I GTTCTTCCCG GGGAGGACGAUGCGGCCUUUUU UUUGU UCUUCCCGCAGACGACUCGCCCGA 3473_H04
CATTTTCTGTTTTTTTTCCG GGGAGGACGAUGCGGCAUUU UCUG UUUUUUUUCCGCAGACGACUCGCCCGA 3474JH04
TTTCAATI'CCCC CTTTCCG G6GA6GACGAUGCGGUUUCAAUUCCCCUCUUUCCGCAGAC6ACUCGCCC6A 3475_H04
CATTTTCCTTTCTCCTTCCG GGGAGGACGAUGCGGCAUUUUCCUUUCUCCUUCCGCAGACGACUCGCCCGA 3476_ H04
GTTATATTG GCTTTTTTCCG GGGAGGACGAUGCGGGUUAUAU UGGCUUUUUUCCGCAGACGACUCGCCCGA 3477_H04
ATATTTTGTTTTTTCTTGCCC GGGAGGACGAUGCGGAUAU U UUGUU UUUUCUUGCCCCAGACGACUCGCCCGA 3478_ H04
GATTTTTTCTCTCTTTTCCC GGGAGGACGAUGCGGGAUUUUUUCUCUCUUUUCCCCAGACGACUCGCCCGA 3479„H04
ATTTTTCCTTACCCTCTCCG GGGAGGACGAUGCGGAUUU UUCCUUACCCUCUCCGCAGACGACUCGCCCGA 3480_H04
CACTTTGTTCTTTTCTCCCG GGGAGGACGAUGCGGCACUUUGUUCUUUUCUCCCGCAGACGACUCGCCCGA 3481_HQ4
ATCTTTAG CTTTTTACACCG GGGAGGACGAUGCGGAUCUU UAGCUUUUUACACCGCAGACGACUCGCCCGA 3482_H04
TCATTCACTTTTTCTTTCCC GGGAGGACGAUGCGGUCAUUCACUUUUUCUUUCCCCAGACGACUCGCCCGA 3483_H04
TG I I Ί I t Π TCCCTTTCCCCG GGGAGGACGAUGCGGUG UUUUUUUUUCCCUUUCCCCGCAGACGACUCGCCCGA 3484_H04
TTTGGGAGTAGGGTGTTCAG 6GGAGGAC6AUGC6GUU UGGGAGUAGGG UGUUCAGCAGACGACUCGCCCGA 3485_H04
ATTCTTCTA I I I I i I CACCG GGGAGGACGAUGCGGAU UCUUCUAU UUUUUCACCGCAGACGACUCGCCCGA 3486_H04
CTTTTATTCACTTTTTCCG GGGAGGACGAUGCGGCUUUUAUUCACUUUUUCCGCAGACGACUCGCCCGA 3488_ HG4
TTTTCTTTGTTTTT AC G CG C GGGAGGACGAUGCGGUU UUCUUUGUUUUUACGCGCCAGACGACUCGCCCGA 3489 H04
« in H4
HTS read variable region RNA sequences lection selection Selected sequenc GCTTTTTTACTTTCTTACCG GGGAGGACGAUGCGGGCUUUU UUACUUUCUUACCGCAGACGACUCGCCCGA 3490 HQ4
ATTTATCTATT7TTTTTGCTG GGGAGGACGAUGCGGAUUUAUCUAUUUUUUUUGCUGCAGACGACUCGCCCGA 3491_H04
TCTCTTTA TTTTTTTCTTCCG GGGAGGACGAUGCGGUCUCUUUAU UUUUUUCUUCCGCAGACGACUCGCCCG 3492_H04
CCTTATTTTTTG CCTTCCC G GGGAGGACGAUGCGGCCUUAUU UUUUGCCUUCCCGCAGACGACUCGCCCGA 3493_HQ4
TTTGTCAGCTTT 1 ΓΠ HTCCG GGGAGGACGAUGCGGUUUCUCAGCUUUUUUUUUUCCGCAGACGACUCGCCCGA 3494_HG4
CTTTTG G CTT ATTTTTCCCG GGGAGGACGAUGCGGCUUU UGGCUUAUUUU UCCCGCAGACGACUCGCCC6A 3495_K04
TTTTTTATATCG CGTTTCCG GGGAGGACGAUGCGGUUUUUUAUAUCGCGUUUCCGCAGACGACUCGCCCGA 3496_H04
TTACTTTTCTTTTCTTCCCC GGGAGGACGAUGCGGU UACUUUUCUUUUCUUCCCCCAGACGACUCGCCCGA 3497„H04
TTTTCTATCTTTTGTCCG GGGAGGACGAUGCGGU UUUCUAUCUUUUGUCCGCAGACGACUCGCCCGA 3498_H04
CTTTTTTTTTTTTCCCACCG GGGAGGACGAUGCGGCU UUUUUUUUUIJUCCCACCGCAGACGACUCGCCCGA 3499_H04
TG ACTCAATTTTTTCCG GGGAGGACGAUGCGGUGUUCACUCAAUUUUU UCCGCAGACGACUCGCCCGA 3500_H04
ΤΤΤΑπΤΤΤ'1'TCTCGTCCCG GGGAGGACGAUGCGGUUUAUUUUUUUCUCGUCCCGCAGACGACUCGCCCGA 3SQ1_H04
CTTTTTGTTTTACTTTTGTG GGGAGGACGAUGCGGCUUUUUGUUUUACUUUUGUGCAGACGACUCGCCCGA 3502J-W4
CTTTCTTTTCCACTTTTCCG GGGAGGACGAUGCGGCUUUCUUUUCCACUUUUCCGCAGACGACUCGCCCGA 3503_H04
CCATTTGTTCTTTTTCTTCCG GGGAGGACGAUGCGGCCAUUUGUUCUUUUUCUUCCGCAGACGACUCGCCCGA 3504_H04
CTTCACTTTTTTTCTCTCCG GGGAGGACGAUGCGGCUUCACUUUUUUUCUCUCCGCAGACGACUCGCCCGA 3505„H04
ATTTTTGGTATTTTTTTACCG GGGAGGACGAUGCGGAUUUUUGGUAUUUUUUUACCGCAGACGACUCGCCCGA 3506„H04
TTTACTCGTTATTTTTGCCG GGGAGGACGAUGCGG UUUACUCGUUAUUUUUGCCGCAGACGACUCGCCCGA 3507_H04
ATCTTGCTGGTTTTTTTACCG GGGACCACGAUGCGGAUCUUGCUGCUUUUUU UACCGCAGACGACUCGCCCGA 3508_H04
ATTTGCTCCTTTTTTTCCCG GGGAGGACGAUGCGGAUUUGCUCCUUUUUUUCCCGCAGACGACUCGCCCGA 3509__H04
AGTTTTTTTATTTGTTGCC GGGAGGACGAUGCGGAGUU UUUUUAUUUGUUGCCGAGACGACUCGCCCGA 3510_H04
GTTGGTGTAG G G GG GTCCG GGGAGGACGAUGCGGGUUGGUGUAGGGGGGUCCGCAGACGACUCGCCCGA 3511JH04
TTCGAAGGGCGGTGGGTCCG GGGAGGACGAUGCGGUUCGAAGGGCGGUGGG UCCGCAGACGACUCGCCCGA 3513_ HQ4
TTTG A AG G GTG ATGTTCCG GGGAGGACGAUGCGGUUUGAAGGG U6AUGUUCCGCAGACGACUCGCCCGA 3514_H04
CATAGGAGTAGGGTGGTACCG GGGA6GACGAUGCGGCAUAGGAGUAGGGUGGUACGGCAGACGACUCGCCCGA 3515„H04
TCCTTGTCGTCTTCGTCCC GGGAGGACGAUGCGGUCCUUGUCGUCUUCG UCCCCAGACGACUCGCCCGA 3516 H04
# irt H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTCTTGTCGTCTTGCGTCCC GGGAGGACGAUGCGGUUCU UGUCG UCUUG G UCCCCAGACQACUCGCCCGA 3517_ H04
AACCTCCTCAGTCGTGTGGC GGGAGGACGAUGCGGAACCUCCUCAGUCGUGUGGCCAGACGACUCGCCCGA 3519_H04
TCCTGTCGTCTGTTCTCCCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCUCCCCCAGACGACUCGCCCGA 3520„H04
TCCTTTCGTTCTGCCCCCC GGGAGGACGAUGCGGUCCUUUCGUUCUGCCCCCCCAGACGACUCGCCCGA 3521_H04
TCTGCTGTGCTTGATATGCCC GGGAGGACGAUGCGGUCUGCUGUGCUUGAUAUGCCCCAGACGACUCGCCCGA 3522_H04
ACTTTTTTCTCTCTTTTCCG GGGAGGACGAUGCGGACUUUUUUCUCUCUUUUCCGCAGACGACUCGCCCGA 3523_H04
ATCCTTTACTTTCTTTGCCC GGGAGGACGAUGCGGAUCCUUUACUU UCUUUGCCCCAGACGACUCGCCCGA 3525_H04
TTCTTCTTCTATTCTCACCG GGGAGGACGAUGCGGUUCUUCUUCUAUUCUCACCGCAGACGACUCGCCCGA 3528_H04
ATTTTCTCTTATT PGCCC GGGAGGACGAUGCGGAUUU UCUCU UAUUCUUGCCCCAGACGACUCGCCCGA 3529_H0
TTTTTCCCTTTCCTCTGCCG GGGAGGACGAUGCGG UUUUUCCCUUUCCUCUGCCGCAGACGACUCGCCCGA 3531_H04
GATTTCTCTTGCCTTTGCCC GGGAGGACGAUGCGGGAU UUCUCUUGCCU UUGCCCCAGACGACUCGCCCGA 3532_H04
CTTTTCTC ! i I i I ! I ! CCGCGC GGGAGGACGAUGCGGCUUU UCUCUUUUUUUUCCGCGCCAGAC6ACUCGCCCGA 3533_H04
GCCTCTCGTTCTTCCCTCCC GGGAGGACGAUGCGGGCCUCUCGUUCUUCCCUCCCCAGACGACUCGCCCGA 3534_H04
TT TTCnTTCCTTTGTGCG GGGAGGACGAUGCGGUUCU UCUUUUCCUUUGUCCGCAGACGACUCGCCCGA 3535_HG4
TCTATTT6GTACTTTTTTCCG GGGAGGACGAUGCGGUCUAUUUGGUACUUUUUUCCGCAGACGACUCGCCCGA 3536_H04
CCACACTTTTCCTCTTTCCG GGGAGGACGAUGCGGCCACACUU UUCC.UCUUUCCGCAGACGACUCGCCCGA 3537„H04
CCTTACATATTCCTCTTCCG GGGAGGACGAUGCGGCCUUACAUAUUCCUCUUCCGCAGACGACUCGCCCGA 3538_H04
TCTCTATGTTCGCTTCTCCG GGGAGGACGAUGCGGUCUCUAUG UUCGCUUCUCCGCAGACGACUCGCCCGA 3539„HG4
GTTTATGGAGGAGGTT6GTG GGGAGGACGAUGCGGGUUUAUGGAGGAGGU UGGUGCAGACGACUCGCCCGA 3540._H04
CCTTTTACTTTTTCTCCG GGGAGGACGAUGCGGCCUU UUACUU U UUCUCCGCAGACGACUCGCCCGA 3542„H04
CCTTTGTTCCTGTATTTCCG GGGAGGACGAUGCGGCCUUUGUUCCUGUAUUUCCGCAGACGACUCGCCCGA 3543_.H04
ACTTTTTTTTTGTCTTCTGCCG GGGAGGACGAUGCGGACUUUUUUUUUGUCUUCUGCCGCAGACGACUCGCCCGA 3544_H04
CCTTTGTA I ! I I I I I I CATCCG GGGAGGACGAUGCGGCCUUUGUAUUUUUUUUCAUCCGCAGACGACUCGCCCGA 3547_H04
ATC TCG TTTCACTTTCACCG GGGAGGACGAUGCGGAUCUCGU UUCACUUUCACCGCAGACGACUCGCCCGA 3548_H04
TTTATTCGGTATTTTCTCCG GGGAGGACGAUGCGCUUUAUUCGC UAUU UUCUCCGCAGACGACUCGCCCGA 3549_H04
TATTTTCCATTCACCTTCCG GGGAGGACGAUGCGGUAUUUUCCAUUCACCUUCCGCAGACGACUCGCCCGA 3550 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc GTCTCACTTTTTTATCTCCG GGGAGGACGAUGCGGGUCUCACUUUUUUAUCUCCGCAGACGACUCGCCCGA 3551_HQ4
TTG I I I I ! I I ATTGTGTCCCG GGGAGGACGAUGCGGUUGUUUUUU UAUUG UGUCCCGCAGACGACUCGCCCGA 3552_H04
TTTTAGCTTTTTTTTGTCCG GGGAGGACGAUGCGGUUUUAGCUUUUUUUUGUCCGCAGACGACUCGCCCGA 3553_H04
AGTATCTATC t I ! I i I ! GCCG GGGAGGACGAUGCGGAGUAUCUAUCUUUUUUUGCCGCAGACGACUCGCCCGA 3557_H04
CT TTTC ATG CTTTCCG GGGAGGACGAUGCGGCUUUUUUUCAUGCUUUCCGCAGACGACUCGCCCGA 3558_H04
TCTT7TT CATTATCTCCCCG GGGAGGACGAUGCGGUCUUUUUU UCAUUAUCUCCCCGCAGACGACUCGCCCGA 3559_H04
GCTTTCTGTCCC ITCCG GGGAGGACGAUGCGGGCUUUCUGUCCCUU UUUCCGCAGACGACUCGCCCGA 356Q_HQ4
TCTTCGTTTCCTTTTCACCG GGGAGGACGAUGCGGUCUUCGUUUCCUUUUCACCGCAGACGACUCGCCCGA 3561_H04
ATCTTTiTTTCTCACCTGCG GGGAGGACGAUGCGGAUCUUUUUUUCUCACCUCCGCAGACGACUCGCCCGA 3562_ H04
TTTTTTGTGTTTCTTGCCCC GGGAGGACGAUGCGGU UUUUUGUGU UUCUUGCCCCCAGACGACUCGCCCGA 3563_H04
TTTTTTTTG T CTTTCTTCCG GGGAGGACGAUGCGGUU'JUUUUUGUCUUUCUUCCGCAGACGACUCGCCCGA 3564_H04
TTT6CCCTTATCTTTTTCCG GGGAGGACGAUGCGGUUUGCCCUUAUCUUUUUCCGCAGACGACUCGCCCGA 3565_H04
TTTTGTTCTCTTTTCTTCCG GGGAGGACGAUGCGGUUUUGUUCUCUUUUCUUCCGCAGACGACUCGCCCGA 3566„H04
TTCGTAC ' M i l l ! I CCG GGGAGGACGAUGCGGUUCG UACUUUUUUUUCCGCAGACGACUCGCCCGA 3567„H04
CTTTTTAAACTCTCACTCCG GGGAGGACGAUGCGGCUUUUUAAACUCUCACUCCGCAGACGACUCGCCCGA 3568_ H04
CCTTTTACTTT'l'TTCCTCCG GGGAGGACGAUGCGGCCUUUUACUUUUUUCCUCCGCAGACGACUCGCCCGA 3559_H04
GTCTTTACTGTTTCTCCCCG GGGAGGACGAUGCGGGUCU UUACUGUUUCUCCCCGCAGACGACUCGCCCGA 3570_H04
TCCATTTTCTTTTCTCTCCG GGGAGGACGAUGCGGUCCAUUUUCUUUUCUCUCCGCAGACGACUCGCCCGA 3572_H04
CCTTGTTTGTTTTTTTCCCG GGGAGGACGAUGCGGCCUUGUUUGUUUUUUUCCCGCAGACGACUCGCCCGA 3573_H04
CCTTTACTTTTTATTCCCGC GGGAGG ACGAUGCGGCCUU U ACUU U U UAU UCCCGCCAGACGACUCGCCCGA 3574_KG4
TTTCTTGATTTTTTCCTCGG GGGAGGACGAUGCGGUUUCUUGAU UUUUUCCUCGGCAGACGACUCGCCCGA 3576_H04-
CTTTCTCTTGTCTTATACCG GGGAGGACGAUGCGGCUUUCUCUUGUCUUAUACCGCAGACGACUCGCCCGA 3577_H0
ACTTATTTG TTTTCTTCCTG GGGAGGACGAUGCGGACUUAUUUGUUUUCUUCCUGCAGACGACUCGCCCGA 3578_H04
TTACTTTCTAATTTTCCGGCCG GGGAGGACGAUGCGGU UACUUUCUAAUUUUCCGGCCGCAGACGACUCGCCCGA 3579_H04
TTCTTTCTATATTTTTTCCG GGGAGGACGAUGCGGU UCUUUCUAUAUUUUUUCCGCAGACGACUGGCCCGA 3580_H04
ACTTTTTATTCATTTCCCTG GGGAGGACGAUGCGGACUUUUUAUUCAUUUCCCUGCAGACGACUCGCCCGA 3581 H04
U in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc ACTTTATACTTGTTTCCCCG GGGAGGACGAUGCGGACUUUAUACUUGU UUCCCCGCAGACGACUCGCCCGA 3582 HQ4
TCTTTTTATCTTCATTCCC6 GGGAGGACGAUGCGG UCUUUU UAUCUUCAUUCCCGCAGACGACUCGCCCGA 3583J-IQ4
fi ATCTnCACmTTCCG GGGAGGACGAUGCGGAUUAUCUUUCACUU UUUCCGCAGACGACUCGCCCGA 35S4_HQ4
CTTTGTCTTAATTTCTACCG GGGAGGACGAUGCGGCUUUGUCUUAAU UUCUACCGCAGACGACUCGCCCGA 3585_H04
TATTCATTTCTCTTTTACG C GGGAGGACGAUGCGGUAU UCAUUUCUCUUUUACGCCAGACGACUCGCCCGA 586„HQ4
CTCTATACAATTTCTTTCCG GGGAGGACGAUGCGGCUCUAUACAAUU UCU UUCCGCAGACGACUCGCCCGA 3587__H04
TTACTTTAATTC M i l l GTG GGGAGGACGAUGCGGU UACUUUAAUUCUUUU UGUGCAGACGACUCGCCCGA 3588_H04
TTTTTAATTTTTCGTTCCC6 GGGAGGACGAUGCGGU UUU UAAUUUUUCGUUCCCGCAGACGACUCGCCCGA 3589_H04
TTTTCTCTCTT'TTCTCACCC GGGAGGACGAUGCGGUUUUCUCUCUUUUCUCACCCCAGACGACUCGCCCGA 3590_H04
TCATTTCTTTTTTCCCAGTG GGGAGGACGAUGCGGUCAUUUCUUUUUUCCCAGUGCAGACGACUCGCCCGA 3591_H04
TCTTATTCTTTTTTTCCTCTG GGGAGGACGAUGCGGUCUUAUUCUUUUUUUCCLCUGCAGACGACUCGCCCGA 3592_H04
TATCTCTTTTCCTTCGCTG GGGAeGACGAUGCGG'JAUCUUCUUUUCCUUCGCUGCAGACGACUCGCCCGA 3593JHG4
'FTG TTTTTT ATTCT ATTCCG G6GAGGACGAUGCGGUUGUUUUUUAUUCUAUUCCGCAGACGACUC6CCCGA 3594_ H04
CATTTTATCCTGTTCATCCG GGGAGGACGAUGCGGCAUUUUAUCCUGUUCAUCCGCAGACGACUCGCCCGA 3595_H04
T ATATTCCTTG TTTTCTCTG GGGAGGACGAUGCGGUAUAUUCCUUGUUUUCUCUGCAGACGACUCGCCCGA 3596_H04
ATTTATCAATTCTTTCTACCC GGGAGGACGAUGCGGAU UUAUCAAUUCUUUCUACCCCAGACGACUCGCCCGA 3S97_H04
CTTCTGTCTCTI'ATTTTCCG GGGAGGACGAUGCGGCUUCUGUCUCUUAUUUUCCGCAGACGACUCGCCCGA 3598_H04
TATCTTTTCTCCTTTGGCCG GGGAGGACGAUGCGGUAUCUUU UCUCCUUUGGCCGCAGACGACUC6CCCGA 3599_H04
TTTTTCTATCCrrTTCCACTG GGGAGGACGAUGCGGUUUU UCUAUCCUUU UCCACUGCAGACGACUCGrrCGA 3600_H04
ACTTACTGTTTACTTTACCG GGGAGGACGAUGCGGACUUACUGUUUACUUUACCGCAGACGACUCGCCCGA 3601_H04
CACTTTTTTTCTTTATCCCC GGGAGGACGAUGCGGCACUUUUUUUCUUUAUCCCCCAGACGACUCGCCCGA 3602JH04
TTATCACTTTCTTTTTACGC GGGAGGACGAUGCGGUUAUCACUUUCUUU UUACGCCAGACGACUCGCCCGA 3603JH04
TCTTTTCTTTTTTAGCTCCC GGGAGGACGAUGCGGUCUUUUCUUUUUUAGCUCCCCAGACGACUCGCCCGA 36Q4JH04
TTTCTATTTGTCTTCATGTG GGGAGGACGAUGCGG UU UCUAUUUGUCUUCAUGUGCAGACGACUCGCCCGA 3605 H04
A ACTTTTTCCTTTATTTC CG GGGAGGACGAUGCGGAACUUUUUCCUUUAUUUCCGCAGACGACUCGCCCGA 3606_H04
TATTGTGATCTTT1 TTTCCG GGGAGGACGAUGCGGUAUUCUGAUCU UUUUU UUCCGCAGACGACUCGCCCGA 3608 H04
# in H4
HTS read variable region RNA sequences selection selection Selected sequenc
TTTTAGC 11 1 1 1 Ϊ TTTTGTCCG GGGAGGACGAUGCG6U UUUAGCUUUUUUUUUUGUCCGCAGACGACUCGCCCGA 3609mH04
ATTACTTGGTTTTTTTCACCG GGGAGGACGAUGCGGAUUACUUGGUUUUUUUCACCGCAGACGACUCGCCCGA 3610 H04
TTCTTAATTACTCTCTCCCG GGGAGGACGAUGCGGU UCUUAAUUACUCUCUCCCGCAGACGACUCGCCCGA 3611 H04
CATTCTTTTCGCTTTTTCCG GGGAGGACGAUGCGGCAUUCUUUUCGCUUUUUCCGCAGACGACUCGCCCGA 3612„H04
AC C ATT ATTTTTCT ATTCC C GGGAGGACGAUGCGGACCAUUAUUUUUCUAUUCCCCAGACGACUCGCCCGA 3613_H04
ACTTTTTG CTGTTTTTTTCCC GGGAGGACGAUGCGGACUUUUUGCUGUUUUUUUCCCCAGACGACUCGCCCGA 3514_H04
TTTACTATTTATTCTGTCTG GGGAGGACGAUGCGGUUUACUAUUUAUUCUGUCUGCAGACGACUCGCCCGA 3615_HQ4
GTCT'TTAAATCTCTTTTCCC GGGAGGACGAUGCGGGUCU UUAAAUCUCUUUUCCCCAGACGACUCGCCCGA 3616_ H04
ACTTTACTCTTCTTTACCG GGGAGGACGAUGCGGACUUUACUCUUCUUUACCGCAGACGACUCGCCCGA 3617„H04
TTTCTACACAACCTTTTCCG GGGAGGACGAUGCGGUUUCUACACAACCUUUUCCGCAGACGACUCGCCCGA 3618 JHQ4
TCACTTTTATCTCTTTCTG GGGAGGACGAUGCGGUCACU UU UAUCUCUUUCUGCAGACGACUCGCCCGA 3619_HG4
A TTTcc i n u t i TTTCCCGc GGGA6GACGAUGCGGAUUUUCCUUUUUUUUUUCCCGCCAGACGACUCGCCCGA 3620.. H04
TACTTATTTCTTACTCTCTG GGGAGGACGAUGCGGUACU UAUUUCUUACUCUCUGCAGACGACUCGCCCGA 3621_H04
TTTTTTCTCTTTTTTCCGCCG GGGAGGACGAUGCGGU UUUUUCUCUUUUUUCCGCCGCAGACGACUCGCCCGA 3622JHQ4
CCGTTATTCTAGTTTTTCCG G GG A GG ACGAUG CG G CCG U U AU UCU AG U U U U UCCG CAG ACG AC U CG CCCG A 3624_H04
τττσπΰτεΑ'Πϋ i I CCG GGGAGGACGAUGCGGU UUCUUGUCAUUUUU UCCGCAGACGACUCGCCCGA 3625_ H04
AGTTTTTCACTTATTTTCCG GGGAGGACGAUGCGGAGUU UUUCACUUAUUUUCCGCAGACGACUCGCCCGA 3627_H04
TTTCAATTTCCCTCTTTCCG GGGAGGACGAUGCGGU UUCAAU UUCCCUCUUUCCGCAGACGACUCGCCCGA 3628„H04
CTTCATAATCTTTGTTTCCG GGGA6GACGAUGCGGCU UCAUAAUCUUUCUUUCCGCAGACGACUCGCCCGA 3629,_H04
GTTTTG CTCCT7TTTCACCG GGGAGGACGAUGCGGGU UUUGCUCCUUUUUCACCGCAGACGACUCGCCCGA 3630„H04
TATCTTTATCCCTATTTCCG GGGAGGACGAUGCGGUAUCUUUAUCCCUAUUUCCGCAGACGACUCGCCCGA 3631_H04
TTTTCCTTAATCCTTTCCCG GGGAGGACGAUGCGGUUUUCCU UAAUCCUUUCCCGCAGACGAOJCGCCCGA 3633_H04
TTACTTTCTCTTTTCTTG TC GGGAGGACGAUGCGGUUACU UUCUCUUUUCU UGUCCAGACGACUCGCCCGA B634J 04
TATTCATTTCTATCTTTCCC GGGAGGACGAUGCGGUAUUCAU UUeUAUCUUUCCCCAGACGACUCGCCCGA 3635_H04
TmGCTTTTTTTTCCCC GGGAGGACGAUGCGGUUUUGCU UU UUU UUCCCCCAGACGACUCGCCCGA 3636J-I04
ATACTTTAACTCTCTTCCCG GGGAGGACGAUGCGGAUACUUUAACUCUCUUCCCGCAGACGACUCGCCCGA 3637 H04
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# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc GTTATTGCTCCTTTTTGCCG GGGAGGACGAUGCGGGUUAUUGCUCCUUUU UGCCeCAGACGACUCGCCCGA 3669. _HQ4
ATTCATTTCTTTTCTTGCCG GGGAGGACGAUGCGGAUUCAUUUCUUUUCUUGCCGCAGACGACUCGCCCGA 367Q. H04
TTTCTCTCTTGCTCGTTCCG GGGAGGACGAUGCGGUUUCUCUCUU6CUC6UUCCGCAGACGACUCGCCCGA 3671. _HQ4
TCTTTCACTGTCTTTTGCCG GGGAGGACGAUGCGGUCUU UCACUGUCUU UUGCCGCAGACGACUCGCCCGA 3672. .H04
ACCTTTTCTTCCTATTCCG GGGAGGACGAUGCGGACCUU UUCUUCCUAUUCCGCAGACGACUCGCCCGA 3673. H04
ATCGTTTACTGTTTTTGCCG GGGAGGACGAUGCGGAUCGUUUACUGUU UU UGCCGCAGACGACUCGCCCGA 3674. _H04
CTTATTTTACTCTTTCTGCC GGGAGGA GAUGCGGCU UAUUUUACUCUUUCUGCCCAGACGACUCGCCCGA 3675., . H04
ATGCTTGGAGCAGGGAGGTG GGGAGGACGAUGCGGAUGCU UGGAGCAGGGAGGUGCAGACGACUCGCCCGA 3676. .H04
TTTGGGGGTAGGGGGTTCCG 6GGAGGACGAUGCGGUUUGGGGGUAGGGGGUUCCGCAGACGACUCGCCCGA 3677. _H04
CG'TTTCATTTTTCCCTTGCC GGGAGGACGAUGCGGCGUUUCAUUUUUCCCUUGCCCAGACGACUCGCCCGA 3678. .H04
TTTTCCAG G TTTTTTTCCCG C GGGAGGACGAUGCGGUUU UCCAGGUUUUUUUCCCGCCAGACGACUCGCCCGA 3679. .H04
ATTATTCCTCTTTTT6TCCG GGGAGGACGAUGCGGAU UAUUCCUCUUUU UGUCCGCAGACGACUCGCCCGA 3680. _H04
TTTCCTGTATTTCTCATCCG GGGAGGACGAUGCGGUUUCCUGUAUUUCUCAUCCGCAGACGACUCGCCCGA 3681_ ^H0
TTTTTAGTTTGT1 TTCCCG C GGGAGGACGAUGCGGU UUUUAGU UUG UUUUCCCGCCAGACGACUCGCCCGA 3682. .H04
ATTCTTGG 1 1 i I i I I 1 1 I GCCG GGGAGGACGAUGCGGAU UUGCU UUUUUU UUUGCCGCAGACGACUCGCCCGA 3683. .H0.4
CCTTTATCTCTTTG CCTCCG GGGAGGACGAUGCGGCCUUUAUCUCUUUGCCUCCGCAGACGACUCGCCCGA 3685. _H04
TTTGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 3686. H04
ACTTACTTATTTCCCTTCCG GGGAGGACGAUGCGGACUUACUUAUU UCCCUUCCGCAGACGACUCGCCCGA 3687. _H04
CTTTTTCCATCTTATTACCG GGGAGGACGAUGCGGCU UUUUCCAUCUUAUUACCGCAGACGACUCGCCCGA 3688. _H04
TCTTTTACTG CTTATTTCCG GGGAGGACGAUGCGGUCUUUUACUGCUUAUUUCCGCAGACGACUCGCCCGA 3689. _H04
TCTTTATTCCTCTGCTTCCG GGGAGGACGAUGCGGUCUU UAUUCCUCUGCUUCCGCAGACGACUCGCCCGA 3690. .H04
ATCrrCGTCTATTTTTTCCG GGGAGGACGA'JGCGGAUCUUCGUCUAUUU UUUCCGCAGACGACUCGCCCGA 3691. .H04
C ATTACCTG TCTTTTCTCC G GGGAGGACGAUGCGGCAUUACCUGUCUUUUCUCCGCAGACGACUCGCCCGA 3692. „H04
ACTTTCTTCTCTTTGCCG GGGAGGACGAUGCGGACU UUCUUCUCUUUGCCGCAGACGACUCGCCCGA 3693. _H04
TTTGG GGTTTTCCGTG CCG GGGAGGACGAUGCGGUU UGGGG UUUUCCGUGCCGCAGACGACUCGCCCGA 3694. _H04
TCCTGG CTTGTCGTTG TTCC GGGAGGACGAUGCGGUCCUGGCU UGUCGUUGUUCCCAGACGACUCGCCCGA 3695. .H04
# in H4
UTS read variable region RNA sequences # in H3 selection selection Selected sequenc CCTTTACTTCTATCTTACCG GGGAGGACGAUGCGGCCUUUACUUCUAUCUUACCGCAGACGACUCGCCCGA 3696_HQ4
TTTCTACACTCCTTTTTCCG GGGAGGACGAUGCGGUUUCJACACUCCUUUUUCCGCAGACGACUCGCCCGA 3697_H04
ACCTTTTCCATTTTTTTCCG GGGAGGACGAUGCGGACCUUUUCCAUUUUUUUCCGCAGACGACUCGCGCGA 3698_H04
AATTTTTGTTCCCTGTACCG GGGAGGACGAUGCGGAAUU UU UGUUCCCUGUACCGCAGACGACUCGCCCGA 3699__H04
TTTCTTTTTTTATCCCCGTG GGGAGGACGAUGCGGUUUCUU UUUUUAUCCCCGUGCAGACGACUCGCCCGA 3700_H04
TCATTTCTTTCTCG CCTCCG GGGAGGACGAUGCGGUCAUUUCUU UCUCGCCUCCGCAGACGACUCGCCCGA 3701_H04
ACGCTTTCTCCITTTCTCCG GGGAGGACGAUGCGGACGCU UUCUCCU UUUCUCCGCAGACGACUCGCCCGA 3702„HG4
TTTTTGTTTTGTTTTTTGGTG GGGAGGACGAUGCGGUUUUUG UUUUGUUUUUUGGUGCAGACGACUCGCCCGA 3703_H04
TCTTTATGTTCCTTTTTCCC GGGAGGACGAUGCGGUCUU UAUGUUCCU UUUUCCCCAGACGACUCGCCCGA 37Q4JHG4
cTTTrcTTTTTTTr GCGc GGGAGGACGAUGCGGCUUUUCUUUUUUUUCCGCGCCAGACGACUCGCCCGA 3705JH04
CATTTCTTCTTCTTTCAGCC GGGAGGACGAUGCGGCAUUUCUUCUUCUU UCAGCCCAGACGACUCGCCCGA 3706_H04
TTTTG TTTTTCTCTTTTT'GCG GGGAGGACGAUGCGGU UUUGUUUUUCUCUUUUUCCGCAGACGACUCGCCCGA 3708_H04
TTTGGGAGTAGGGTGTTACG GGGAGGACGAUGCGGU UUGGGAGUAGGGUGUUACGCAGACGACUCGCCCGA 3709_H04
ATGCTTGGAGTAGGGTGGTG GGGAGGACGAUGCGGAUGCUUGGAGUAGGGUGGUGCAGACGACUCGCCCGA 3711_ H04
CTTTTTTCATTCGCGTTCCG GGGAGGACGAUGCGGCUUUUU UCAUUCGCGU UCCGCAGACGACUCGCCCGA 3712_H04
ATfTTTCTTCTTCTTTTACCG GGGAGGACGAUGCGGAUUUUUCUUCUUCUUUUACCGCAGACGACUCGCCCGA 3716_H04
CTCATTTTTTTCCGTCTCCG G6GAGGACGAUGCGGCUCAUUUUUUUCCGUCUCCGCAGACGACUCGCCCGA 3717_H04
TCTTTTTATCATCTTTCCTG GGGAGGACGAUGCGGUCUU UUUAUCAUCUUUCCLGCAGACGACUCGCCCGA 3718raH04
CTTTTTACTGTTCTTTTCTG GGGAGGACGAUGCGGCUUU UUACUGUUCUUUUCUGCAGACGACUCGCCCGA 3719J-i04
TTTCCATATTTACTTTCCCG GGGAGGACGAUGCGGU UUCCAUAUUUACU UUCCCGCAGACGACUCGCCCGA 3720J G4
CTTTATCG TGTTTCTTAC CG GGGAGGACGAUGCGGCUUUAUCGUGUUUCUUACCGCAGACGACUCGCCCGA 3721_HG4
CTTACTCACTACTTTCTCCG GGGAGGACGAUGCGGCU UAeUCACUACUUUCUCCGCAGACGACUCGCCCGA 3722JH04
GGTTCnTTTTCTCCATCCC GGGAGGACGAUGCGGGG UUCUUUUUUCUCCAUCCCCAGACGACUCGCCCGA 3?23_HG4
TACCACT TTATTTTTCCCCG GG6AGGACGAUGCGGUACCACUUUAUU UUUCCCCGCAGACGACUCGCCCGA 372 _H04
TTTTTCAGTCTATTTTTCCG GGGAGGACGAUGCGGUUUUUCAGUCUAUUUUUCCGCAGACGACUCGCCCGA 3725_HQ4
ATTCTTTTACTCTGCTACCG GGGAGGACGAUGCGGAUUCU UUUACUCUGCUACCGCAGACGACUCGCCCGA 3726 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CTTTTTCG TTTTTTTCTTCCG GGGAGGACGAUGCGGCUUU UUCGUUUUUUUCUUCCGCAGACGACUCGCCCGA 3727. _HG4
ATTTTT6TATTC 1 1 i I N GCCC GGGAGGACGAUGCGGAUU UUUGUAUUCUUU UUUGCCCCAGACGACUCGCCCGA 3728. .H04
CCATTTACACTTATTTTCCG GGGAGGACGAUGCGGCCAUU UACACUUAUUUUCCGCAGACGACUCGCCCGA 3729. _H04
TTTTTGTTAATCCTTTTGCC GGGAGGACGAUGCGGU UUUUGUUAAUCCUU UUGCCCAGACGACUCGCCCGA 3730. .H04
AACTTTACTTTCCCTTTCCC GGGAGGACGAUGCGGAACUUUACUU UCCCUUUCCCCAGACGACUCGCCCGA 3731, _H04
CTTCTTTTCTCGCTTTCCG GGGAGGACGAUGCGGCU UCUUUUCUCGCU UUCCGCAGACGAC UCGCCCGA 3732 _H0
TATTTTTATTTCTTTGTCTG GGGA6GACGAUGCGGUAUU UUUAU UUCUUUGUCUGCAGACGACUCGCCCGA 3734. H04
TTACTτTTAAAπCGCTCTG GGGAGGACGAUGCGGUUACUUUUAAAUUCGCUCUGCAGACGACUCGCCCGA 3735. H04
TCCTTTACATTTTCTTTCGG GGGAGGACGAUGCGGUCCUU UACAUUUUCUUUCCGCAGACGACUCGCCCGA 3736. _H04
CTTTAGTTTCATTTTTTCCG GGGAGGACGAUGCGGCUUUAGU UUCAUUUUU UCCGCA6ACGACUCGCCCGA 3737. _HQ4
TTTCTGACACTTTTTTCCG GGGAGGACGAUGCGGU UUCUGACACUUUUUUCCGCAGACGACUCGCCCGA 3738. _HQ4
TGCTTAGGAGGATGGAGGTG GGGAGGACGAUGCGGU6CU U AG GAG G AU G G A G G U GCAG ACG AC U CGCCCGA 3739. _H04
TTTCATGCCCATTCTTTCCG G.GGAGGAGGAUGCGGUUUCAUGCCCAUUCUUUCCGCAGACGACUCGCCC6A 3740. _H0.4
GTGTCTTTCTTTCTTTTCCC GGGAGGACGAUGCGGGUGUCUUUCUUUCUUUUCCCCAGACGACUCGCCCGA 3741. .H04
TCATTTTCTTTTTi'CCCCAC GGGAGGACGAUGCGGUCAUUUUCUUUUU UCCCCACCAGACGACUCGCCCGA 3744. .H04
AGGGATTGGAGGTGTACCCG GGGAGGACGAUGCGGAGGGAUUGGAGGUGUACCCGCAGACGACUCGCCCGA 3745. .H04
TTTATTTTCTTCTCATTTCCT GGGAGGACGAUGCGGUU UAUUUUCUUCUCAUUUCCUCAGACGACUCGCCCGA 3746. _H04
ATTTCTTTCCTTTTTACCG GGGAGGACGAUGCGGAU UUCUUUCCUUUU UACCGCAGACGACUCGCCCGA 3747_ .H04
CTATCCTTGT Π" 11 1 ' I'CTTCCG GGGAGGACGAUGCGGCUAUCCUUGUUUUUUUCUUCCGCAGACGACUCGCCCGA 3748. _H04
C f l i'H I CCCACCATTTCCG GGGAGGACGAUGCGGCU UUUUUCCCACCAUU UCCGCAGACGACUCGCCCGA 3749. H04
ATCG CTCTATTTTTTTCTCCG GGGAGGACGAUGCGGAUCGCUCUAUUUUUUUCUCCGCAGACGACUCGCCCGA 3750. H04
CTTTCTTCGCTTTTTACCG GGGAGGACGAUGCGGCUUUCUUCGCUUUUUACCGCAGACGACUCGCCCGA 3751. _HG4
CTATATCATTTCTCTTTCCG GGGAGGACGAUGCGGCUAUAUCAUUUCUCU UUCCGCAGACGACUCGCCCGA 3752. _H04
CTTTCCAATTCCTTTTTCCG GGGAGGACGAUGCGGCUUUCCAAUUCCUUUUUCCGCAGACGACUCGCCCGA 3753. ,H04
AATTCCTTTGTTTCTTACCG GGGAGGACGAUGCGGAAUUCCUUUGUUUCUUACCGCAGACGACUCGCCCGA 3754. .H04
CCTTTTATT'TTTTTTCTCCG GGGAGGAGGAUGCGGCCUUUUAUUUUUUUUCUCCGCAGACGACUCGCCCGA 3755. _HG4
# in H4
HIS read variable region NA sequences selection Selected sequenc CTTACATTACCCTTTTTCC G GGGAGGACGAUGCGGCUUACAUUACCCUUUUUCCGCAGACGACUCGCCCGA 3756. ,H04
AACTTGI TTTTTTTTACCGC GGGAGGACGAUGCGGAACUUGUUUUU UUUUUACCGCCAGACGACUCGCCCGA 3757. H04
CTACTTACTCTT TTCTCC G GGGAGGACGAUGCGGCUACUUACUCUUAUUCUCCGCAGACGACUCGCCCGA 3758. H0
TCTTTTCACn" ACTITTTCCG GGGAGGACGAUGCGGUCUU UUCACUUACUUUUUCCGCAGACGACUCGCCCGA 3759_ .H04
CTTGTCACACTCTTTTTCCG GGGAGGACGAUGCGGCUUGUCACACUCUUUUUCCGCAGACGACUCGCCCGA. 3761. H04
CCAATTATTCTTTTCTACCG GGGAGGACGAUGCGGCCAAU UAUUCUUUUCUACCGCAGACGACUCGCCCGA 3762. .H04
TCTTTCTCTTATTTACTCCC GGGAGGACGAUGCGGUCUU UCUCUUAUU UACUCCCCAGACGACUCGCCCGA 3763. ma
ATTCTTTTTrfGTrTTTTGCC GGGA6GACGAUGCGGAUUCUUUUUUUG UU UUUJGCCCAGACGACUC6CCCGA 3764_ H04
CTTCTTCTATATACTCTCCG GGGAGGACGAUGCGGCU UCUUCUAUAUACUCUCCGCAGACGACUCGCCCGA 3765„ H04
CTTTCTTTATTCTCTCTTG C GGGAGGACGAUGCGGCU UUCUUUAUUCUCUCUUGCCAGACGACUCGCCCGA 3766„ .H04
TTCTATTAACCTTTTTCCCG GGGAGGACGAUGC6GU UCUAUUAACCUUUUUCCCGCAGACGACUC6CCCGA 3767. .H04
CTTTTTGTTGCTTTATTCCG GGGAGGACGAUGCGGCUUUUUGUUGCUUUAUUCCGCAGACGACUCGCCCGA 3769. .H04
TTFTTACCCTTTTTTTGTCCT GGGAGGACGAUGCGGUUUUUACCCUUUUUUUGUCCUCAGACGACUCGCCCGA 3 70. .H04
ATTTTTTTCTCTTATTT6CCG GGGAGGACGAUGCGGAUUU UUUUCUCUUAUUUGCCGCAGACGACUCGCCCGA 3771. „HQ4
TTTTTCTCTTTTTTTTCCGCCG GGGAGGACGAUGCGGUUUUUCUCU UUU UUUUCCGCCGCAGACGACUCGCCCGA 3772. H04
TTATTTATTTTTTTTCGCCCG GGGAGGACGAUGCGGUUAUUUAUUUUUUUUCGCCCGCAGACGACUCGCCCGA 3774. H04
GATATTrrCTCTTTTTTTGCC GGGAGGACGAUGCGGGAUAUUUUCUCUUUUUUUGCCCAGACGACUCGCCCGA 3775. H04
TTC ATTTG TTTTTTTTCGTG GGGAGGACGAUGCGGUUCAUUUGU UU UUU UUCGUGCAGACGACUCGCCCGA 3776. .H04
ATTTCACTGTCTTTTTTTCCCG GGGAGGACGAUGCGGAUUUCACUGUCU UUUUUUCCCGCAGACGACUCGCCCGA 3777. _H04
TCTGTTTTACi 1 ! I IITTCCC GGGAGGACGAUGCGGUCUGUUU UACUUUUU UU UCCCCAGACGACUCGCCCGA 3778. H04
GCTrrrTATCGTTTTTTCCG GGGAGGACGAUGCGGGCUUUUUAUCGUUUU UUCCGCAGACGACUCGCCCGA 3779. H04
CTTATTAC 1 1 1 I I I i l ! G CC GGGAGGACGAUGCGGCUUAUUACU UUUUUUUUGCCCAGACGACUCGCCCGA 3780. .H04
TCTTTTTTAACTTTTTCTCCG GGGAGGACGAUGCGGUCUUUUUUAACUUUUUCUCCGCAGACGACUCGCCCGA 3781. _HQ4
ACACTTCTCTTTTTCTACCG GGGAGGACGAUGCGGACACUUCUCUUUUUCUACCGCAGACGACUCGCCCGA 3782^ _H04
TCCTG ACGTCTGTTG GTCCC GGGAGGACGAUGCGGUCCUGACG UCUGUUGGUCCCCAGACGACUCGCCCGA 3784. H04
TCCTGTCGTCTGCCCTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGCCCUCCCCAGACGACUCGCCCGA 3785 H04
S in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
TCCTTGTCGTCTTCCGTCCC GGCAGGACGAUGCGGUCCL UGUCGUCUUCCGUCCCCAGACGACUCGCCCGA 3786_H04
TCCTGTCGTCTGCTCG CCCC GGGAGGACGAUGCGGUCCLGUCGUCUGCUCGCCCCCAGACGACUCGCCCGA 3787_H04
TCCTGTCGGCGGTTCGTCCC GGGAGGACGAUGCGGUCCLGUCGGCGGUUCGUCCCCAGACGACUCGCCCGA 3788_H04
ATTCACTTCTCTTCTTGCCG GGGAGGACGAUGCGGAUUCACUUCUCUUCUUGCCGCAGACGACUCGCCCGA 3?89_ H04
AATT ATGTGTTTTTTG ACCG GGGAGGACGAUGCGGAAUUAUGUGUUUUUUGACCGCAGACGACUCGCCCGA 3790_H04
TTTG G G AG TAG G GTGTTCCT GGGAGGACGAUGCGGUUUGGGAGUAGGGUG UUCCUCAGACGACUCGCCCGA 3793^H04
TATCTTCTTCTTTTTCTCCG GGGAGGACGAUGCGGUAUCU UCUUCUUUUUCUCCGCAGACGACUCGCCCGA 3794_H04
ATTTCTTTTCCTTTTTTCCG GGGAGGACGAUGCGGAUUUCUU UUCCUUUUU UCCGCAGACGACUCGCCCGA 3796_HQ4
TATTCCTTGTTCTCTTTGCCG 6GGAGGACGAUGCGGUAUUCCU UGUUCUCUUUGCCGCAGACGACUCGCCCGA 3798_H04
ACTTTC7TCTTTCTTG CCG GGGAGGACGAUGCGGACUU UCU UCU UUCUUGCCGCAGACGACUCGCCCGA 3799_H04
ACTTTATCCTGCAAGTCCC GGGAGGACGAUGCGGACUL UAUCCUGCAAG UCCCCAGACGACUCGCCCGA 3800_H04
AATTATGTGTTTTTTGTG ACTG GGGAGGACGAUGCGGAAUUAUGUGUU UUUUGUGACUGCAGACGACUCGCCCGA 3SQ2„H04
TTCTCTTG TTGTTTTTGCCC 06GAGGACGAUGCGGUUCUCUUGUUGUUUUUGCCCCAGACGACUCGCCCGA 3803_H04
TCTGGGGTTTTCACTGTCCG GGGAGG ACGAUGCGGUCUGGGGUUUUCACU6UCCGGAGACGACUCGCCCGA 3806_ HG4
TTGGGTGTAGGGAGGTTCCG GGGAGG ACGAUGCGGUUGGGUGUAGGGAGGUUCCGCAGACGACUCGCCCGA 3807_ H04
AAG G GTGTAGG GTG GTACCG GGGAGG ACGAUGCGGAAGGGUGUAGGGUGGU ACCGCAGACGACUCGCCCGA 3808_H04
AITTCCTTTCCTTTTTACCG GGGAGG ACGAUGCGGAU UUCCUUUCCU UUUU ACCGCAGACGACUCGCCCGA 3S09_H04
TTCTTTTCCTTCTTTCTCCG GGGAGGACGAUGCGGUUCUUUUCCUUCUUUCUCCGCAGACGACUCGCCCGA 381Q_H04
CCTTTCGTTCTGCCTCCC GGGAGGACGAUGCGGCCU UUCGUUCUGCCUCCCCAGACGACllCGCCCGA 3811_H04
TCCTGTTATTCTCTTCTCCG GGGAGGACGAUGCGGUCCUGUUAUUCUCUUCUCCGCAGACGACUCGCCCGA 3812_H04
TTTTATTATTCTCTGCTGCC GGGAGG ACGAUGCGGUUUUAU UAUUCUCUGCUGCCCAGACGACUCGCCCG A 3813_H04
TTTGTTTTATCTTTTG TCCC GGGAGGACGAUGCGGUUUG UUUUAUCUUUUGUCCCCA6ACGACUCGCCCGA 3815 H04
CCTTTTCTTTCCTATTeCG GGGAGGACGAUGCGGCCUUUUCUUUCCUAUUCCGCAGACGACUCGCCCGA 3816_HG4
GTACTCTTTTCTTACCTCCG GGGAGGACGAUGCGGCUACUCUUUUCUUACCUCCGCAGACGACUCGCCCGA 3817_H04
TTTTACTATTCACTTTCCGC GGGAGGACGAUGCGGUUUUACUAUUCACUUUCCGCCAGACGACUCGCCCGA 3818 H04
TCCAGTTTCTATTTTCTCCG GGGAGGACGAUGCGGUCCAGUUUCUAUUUUCUCCGCAGACGACUCGCCCGA 3819 H04
-
U in H4
HTS read variable region NA sequences selection Selected sequenc ■ATTTTCTCTTTTTTG CCG GGGAGGACGAUGCGGAUUU UCUCUUUUUUGCGGCAGACGACUCGCCCGA 3820_ .H04
T ATC ATT ATTATTTC CTCC G GGGAGGACGAUGCGGUAUCAUUAUUAUUUCCUCCGCAGACGACUCGCCCGA 3821_ H04
TCTTTTCATGTTTTTCTCCG GGGAGGACGAUGCGGUCUU UUCAUGUUUUUGUCCGCAGACGACUCGCCCGA 3822. .H04
ACATCTCACTTTCTCTACCG GGGAGGACGAUGCGGACAUCUCACUUUCUCUACCGCAGACGACUCGCCCGA 3823. .H04
ATCATTTTCCTACTTCTCCG GGGAGGACGAUGCGGAUCAUUUUCCUACUUCUCCGCAGACGACUCGCCCGA 3824_ _H04
GCCTTTCGTTCTGCTTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCUUCCCCAGACGACUCGCCCGA 3825_ _H04
CCTTATCTCTACTCTTTCCG GGGAGGACGAUGCGGCCUUAUCUCUACUCUUUCCGCAGACGAGUCGCCCGA 3826. .H04
GCCTTTCGTTCTCCCCCC GGGAGGACGAUGCGGGCCUUUCGUUCUCCCCCCCAGACGACUCGCCCGA 3827. .H04
GCATTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCAUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 3828. ,H04
ATTACTCTTCTCTTTTTGCCG GGGAGGACGAUGCGGAUUACUCUUCUCUUUUUGCCGCAGACGACUCGCCCGA 3829. .H04
TTCATTTTCTTTCCTCTCCG GGGAGGACGAUGCGGUUCAU UUUCUU UCCUCUCCGCAGACGACUCGCCCGA 3830. _H04
TTTACmTTTCCACGTTCCG GGGAGGACGAUGCGGUUUACUUCUUUCCACGUUCCGCAGACGACUCGCCCGA 3833. _H04
TGTTTTGTTTGCTTTATCCC G6GAGGACGAUGGGGUGUUUUGUU UGCUUUAUCCCCAGACGACUCGCCCGA 3834_ JHG4
GTTTTCCTTCTTTTCGCCCG GGGAGGACGAUGCGGGUUU UCCUUCUUUUCGCCCGCAGACGACUCGCCCGA 3835. -H04
cirmTmcATTTTACCG GGGAGGACGAUGCGGCU UUUCUCUUCAUUUUACCGCAGACGACUCGCCCGA 3836. _H04
CCTTATTACTACCTTTTCCG GGGAGGACGAUGCGGCCU UAUUACUACCUUUUCCGCAGACGACUCGCCCGA 3837. _H04
TTTAITACTACTTCGTTCCG GGGAGGACGAUGCGGUUUAUUACUACUUCGUUCCGCAGACGACUCGCCCGA 3838. .H04
I I I 1 1 1 GATTTTGTTTCCG GGGAGGACGAUGCGGUUUU UUGAU UUUGUUUCCGCAGACGACUCGCCCGA 3839. „H04
TACTTGTTCTACTTTCTCCG GGGAGGACGAUGCGGUACUUGUUCUACUUUCUCCGCAGACGACUCGCCCGA 3840. .H04
ATTCTTGCTTATATrCACCG GGGAGGACGAUGCGGAU UCU UGCUUAUAUUCACCGCAGACGACUCGCCCGA 3841. _H04
CTTACTCTTTTTATTCACCG GGGAGGACGAUGCGGCUUACUCUUUUUAUUCACCGCAGACGACUCGCCCGA 3842_ _H04
CCTTTCTCTTGTTTCTTGCC GGGAGGACGAUGCGGCCUUUCUCUUGUUUCUUGCCCAGACGACUCGCCCGA 3843_ _H04
GATTCTTACTCTTATTTCCC GGGAGGACGAUGCGGGAUUCUUACUCU'JAU UUCCCCAGACGACUCGCCCGA 3844. _HQ4
GCCTTTC6GTCTTCCCTCCG GGGAGGACGAUGCGGGCCUUUCGGUCUUCCCUCCCCAGACGACUCGCCCGA 3847. _H04
ATTACTTCTATTTCTTACCC GGGAGGACGAUGCGGAUUACUUCUAUUUCUUACCCCAGACGACUCGCCCGA 3848. _H04
ACTTTTTTCTTTTCTACCG GGGAGGACGAUGCGGACUUU U UUCUUUUCUACCGCAGACGACtJCGCCCGA 3849 H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc CCATTTTTGTTCCTTTTCCG GGGAGGACGAUGCGGCCAUUUUUGUUCCUUU UCCGCAGACGACUCGCCCGA 3853_H04
TTTCCGGGTTTTTTTTTG CCG GGGAGGACGAUGCGG UUUCCGGGUUUUUU UUUGCCGCAGACGACUCGCCCGA 3854_H04
CTTTTCTCTCTTTTTCCG GGGAGGACGAUGCGGCUUU UCUCUCUUUUUCCGCAGACGACUCGCCCGA 3S55_H04
TCCTTTCGnCTGTTCTCCC GGGAGGACGAUGCGGUCCUUUCGUUCUG UUCUCCCCAGACGACUCGCCCGA 3856_H04
CGTTATTTTCTCCTTGGTCGC GGGAGGACGAUGCGGCGUUAUUUUCUCCUUGGUCGCCAGACGACUCGCCCGA 3857 HG4
CTCATTTTCTCTCCTTTCCG GGGAGGACGAUGCGGCUCAUUUUCUCUCCUUUCCGCAGACGACUCGCCCGA 3858_H04
TTATTmCACTTCTCACGC GGGAGGACGAUGCGGU UAUUU UUCACUUCUCACGCCAGACGACUCGCCCGA 3862_ H04
ATAT rCGTTATTTTTGTGCC GGGAGGACGAUGCGGAUAUUCG UUAUUUUUGUGCCCAGACGACUCGCCCGA 3863_HG4
TATCTTTTGTTTTTCCTCG C GGGAGGACGAUGCG6UAUCUU UUGUUUUUCCUCGCCAGACGACUCGCCCGA 3864_H04
CTTCTTG CAGTC7TTTTCCG GGGAGGACGAUGCGGCUUCUUGCAGUCUU UUUCCGCAGACGACUCGCCCGA 3865_H04
TCTCTTCATGTTTCTCTCCG GGGAGGAGGAUGGGGUCUCUUCAUGUUUCUCUCCGCAGACGACUCGCCCGA 3866..H04
TTTG AAG G G CG ATAG GTCCG GGGAGGACGAUGCGGUUUGAAGGGCGAUAGGUCCGCAGACGACUCGCCCGA 3868_H04
CCTTTACTGTTTCGCTTCCG GGGAGGACGAUGCGGCCUU UACUGUUUCGCUUCCGCAGACGACUCGCCCGA 3869_ H04
TCTCTGTTGTATTTCCTCCG GGGAGGACGAUGCGGUCUCUGUUG UAUU UCCUCCGCAGACGACUCGCCCGA 387G_ H04
ATTCTCTTTCTTTTTACCG GGGAGGACGAUGCGGAUUCUCUUUCU UUUUACCGCAGACGACUCGCCCGA 3871_H04
TACTTTTTTTCCCTTTGCGTG GGGAGGACGAUGCGGUACUUUUUUUCCCUUUGCGUGCAGACGACUCGCCCGA 3872_H04
TTTTTTTTCTTGTTTCCG GGGAGGACGAUGCGGUUUUUUUUCUUGUUUCCGCAGACGACUCGCCCGA. 3873„H04
ATTTTTG ACTTCTTTACC C G GGGAGGACGAUGCGGAUUU UUGACUUCU UUACCCGCAOACGACUCGCCCGA 3874_H04
TTTATTTTCTTCTTTTG CAC GGGAGGACGAUGCGGUUUAUUUUCLIUCU UUUGCACCAGACGACUCGCCCGA 3875..H04
CTATTTTATTGTTTTCTCTG GGGAGGACGAUGCGGCUAUUUUAUUGUUUUCUCUGCAGACGACUCGCCCGA 3876_HG4
TTATCCTG ACT CCGTACGTC GGGAGGACGAUGCGGUUAUCCUGACUCCGUACGUCCAGACGACUCGCCCGA 3877_H04
TCTTTATTTCATTTCC G CCG GGGAGGACGAUGCGGUCUU UAUUUCAUUUCCGCCGCAGACGACUCGCCCGA 3878_H04
CATG CTTTACTTTTTTTCCG GGGAGGACGAUGCGGCAUGCUUUACUUUUUUUCCGCAGACGACUCGCCCGA 3879_H04
ATACGCTCTTATTTTTGCCG GGGAGGACGAUGCGGAUACGCUCUUAU UUUUGCCGCAGACGACUCGCCCGA 388Q_H04
TCTTTAATTTTGCTCCCCG GGGAGGACGAUGCGGUCUUUAAUUUUGCUCCCCGCAGACGACUCGCCCGA 3881_ HG4
TTAGTTTTiATTTTGTTTG GGGAGGACGAUGCGGU UAG UUUUUAUUUUGUUUGCAGACGACUCGCCCGA 3882 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TTTG AACTCTTTTTTTTTTCCG GGGAGGACGAUGCGGUUUGAACUCUUU UUUUU UUCCGCAGACGACUCGCCCGA 3883_H04
TTTTTCTTCTATTC G CTCC A GGGAGGACGAUGCGGU UUUUCUUCUAUUCGCUCCACAGACGACUCGCCCGA 3884_H04
ATTTTGATAATTTTGCTCT6 GGGAGGACGAUGCGGAUUUUGAUAAUUUUGCUCUGCAGACGACUCGCCCGA 3885J-I04
ATTCTGTTGTTTTCCTACCG GGGAGGACGAUGCGGAUUCUGUUGUU UUCCUACCGCAGACGACUCGCCCGA 3SS6_H04
TTTATTGTATTTTTCCTTGG GGGAGGACGAUGCGGUUUAUUGUAUUUUUCCUUGGCAGACGAC'JCGCCCGA 3887_H04
CTTATTTA CG GTTTCTTCCG GGGAGGACGAUGCGGCUUAUUUACGG UU UCUUCCGCAGACGACUCGCCCGA 3888_HG4
CTCTACCTTTTTATCTTCCG GGGAGGACGAUGCGGCUCUACCUUUUUAUCUUCCGCAGACGACUCGCCCGA 3889_H04
TTCT CTCTGTTCACCCG GGGAGCACGAUGCGGUUCU UU UCUCUGU UCACCCGCAGACGACUCGCCCGA 3B90„HG4
ATTT rMCTTTACGCTCCC CGGAGGACGAUGCGGAUUUUUUAACUUUACGCUCCCCAGACGACUCGCCCGA 3891„H04
CTTTCTGTCTTTTACTACCG GGGAGGACGAUGCGGCUUUCUGUCUU UUACUACCGCAGACGACUCGCCCGA 3892_H04
TCACT i 1 t m I 1 CTACCCCG GGGAGGACGAUGCGGUCACUUUUUUUUUCUA.CCCCGCAGACGACUCGCCCGA 3893J-I04
TACTATTTTCTTCACTTGCC GGGAGGACGAUGCGGUACUAUU UUCUUCACUUGCCCAGACGACUCGCCCGA 3894_H04
TACTTTTTCTTTCCTTA CGC GGGAGGACGAUGCGGUACUUUU UCUUUCCUUACGCCAGACGACUCGCCCGA 3895_H04
ACCACTTTTATCCTATTCCG GGGAGGACGAUGCGGACCACUUUUAUCCUAUUCCGCAGACGACUCGCCCGA 3896_H04
ACTTAACTTTTCTCTACCCG GGGAGGACGAUGCGGACUUAACU UUUCUCUACCCGCAGACGACUCGCCCGA 3897_ H04
TTCTTTCTTCTTTTTCTC CG GGGAGGACGAUGCGGUUCUUUCUUCUUUUUCUeCGCAGACGACUCGCCCGA 3898_H04
ATTTCCTTTATCTCATACCG GGGAGGACGAUGCGGAUUUCCU UUAUCUCAUACCGCAGACGACUCGCCCGA 3899_H04
CATTTTACTTTCCTTCTCCG GGGAGGACGAUGCGGCAU.UU UACUUUCCUUCUCCGCAGACGACUCGCGCGA 3902_H04
CT'i'CATCTTTG CTCTTACCG GGGAGGACGAUGCGGCUUCAUCU UUGCUCUUACCGCAGACGACUCGCCCGA 3903_H04
TTTCCACAACTTCTCTTCCG GGGAGGACGAUGCGG UUUCCACAACUUCUCUUCCGCAGACGACUCGCCCGA 3904_H04
G TTT A ATTTATG CTTTTCC6 GGGAGGACGAUGCGGGUUUAAU UUAUGCUUUUCCGCAGACGACUCGCCCGA 3905„H04
TGTTACTCACTTTCTTTCCG GGGAGGACGAUGCGGUGUUACUCACUUUCUUUCCGCAGACGACUCGCCCGA 3906J-I04
TTTCTTTTACGTTTTTTTCCC GGGAGGACGAUGCGGUUUCUUU UACGUUUUUUUCCCCAGACGACUCGCCCGA 3907JH04
1"iTTTCAGTG I ! ί I I IGTGT6 GGGAGGACGAUGCGGUUUU UCAGUGUUUUU UGUGUGCAGACGACUCGCCCGA 3908_H04
TTTTTCGATCTTTGTTCCG GGGAGGACGAUGCGGUUUU UCGAUCUUUGUUCCGCAGACGACUCGCCCGA 3909JHQ4
TTATTCTCTTTCATCTTCCG GGGAGGACGAUGCGGUUAU UCUCUUUCAUCUUCCGCAGACGACUCGCCCGA 3910 H04
# in H4
HIS read variable region RNA sequences # in H3 selection selection Selected sequenc TTACCTTTCTTTTTACTCCG GGGAGGACGAUGCGGUUACCUUUCUUUUUACUCCGCAGACGACUCGCCCGA 3911. _H04
CTTTGTCAACTGTTTTTCCG GGGAGGACGAUGCGGCUUUGUCAACUGUUUUUCCGCAGACGACUCGCCCGA 3912_ _H04
TTCTTTAG TCACTTTTTCCC GGGAGGACGAUGCGGUUCU UUAGUCACUUUUUCCCCAGACGACUCGCCCGA 3913_ .H04
CTCTACTTTC7TACCTTCCG GGGAGGACGAUGCGGCUCUACUUUCUUACCUUCCGCAGACGACUCGCCCGA 3914_ _HQ4
T TCTCTTTTCTCTG CCCGC GGGAGGACGAUGCGGU UUCUCUUUUCUCUGCCCGCCAGACGACUCGCCCGA 3915_ H04
TTTTTCTTTTCCTTGTCCG GGGAGGACGAUGCGGU UUU UCUUUUCCUUGUCCGCAGACGACUCGCCCGA 3916. .H04
TTTTATTCTAACTTTCCCTG GGGAGGACGAUGCGGU UUUAU UCUAACUU UCCCUGCAGACGACUCGCCCGA 3917. _H04
TTGTTTCCTCTTCTTTTCCG GGGAGGACGAUGCGGUUGUUUCCUCUUCUUUUCCGCAGACGACUCGCCCGA 3918. _H04
ATTCTTTTCTTCTCTCTCGC GGGAGGACGAUGCGGAUUCU UUUCUUCUCUCUCGCCAGACGACUCGCCCGA 3920. H04
ACTCTTTCTTCTCTTTTCCG GGGAGGACGAUGCGGACUCU UUCUUCUCUUUUCCGCAGACGACUCGCCCGA 3921. .H04
TTTGGAGGGTGATGGGGCCG GGGAGGACGAUGCGGUUUGGAGGG UGAUGGGGCCGCAGACGACUCGCCCGA 3922. _H04
TTTTTCA CTTTTTTCG CCCCT GGGAGGACGAUGCGGUULIUUCACUUUUUUCGCCCCUCAGACGACUCGCCCGA 3923. -H04
-J TTTTTCTACTTTCCTTTCCG GGGA6GACGAUGCGG UU UU UCUACUU UCCU U UCCGCAG ACGACUCGCCCGA 3924.
NO .H04
CTGTTTCATTCTTTTTTCCC GGGAGGACGAUGCGGCUGUUUCAUUCUUUUUUCCCCAGACGACUCGCCCGA 3325. .H04
TTTATTCTTATTCT7TTG CG GGGAGGACGAUGCGGUUUAUUCUUAUUCUUU UGCGCAGACGACUCGCCCGA 3926. .H04
GCCTTTTCTCCTTTCTACCG GGGAGGACGAUGCGGGCCUU UUCUCCUUUCUACCGCAGACGACUCGCCCGA 3927. H04
TTTCCTGTTTGTTTTCTGCG GGGAGGACGAU6CGGUUUCCUGUUUGUUUUCUGCGCAGACGACUCGCCCGA 3928. ..H04
TTTTTCG G ACTTTTCTCCCG GGGAGGACGAUGCGGUUUU UCGGACUUUUCUCCCGCAGACGACUCGCCCGA 3929. HQ4
TTTTATTTCTACTTCTTTGC CGGAGGACGAUGCGGUUUUAUUUCUACUUCUUUGCCAGACGACUCGQ GA 3930. HQ4
TTTGTTTATTCTCTTTTCCG GGGAGGACGAUGCGGUUUGUUUAUUCUOJUU UCCGCAGACGACUCGCCCGA 393.1. H04
TTCTTTCTATCTCTTCTCCG GGGAGGACGAUGCGGUUCUU UCUAUCUCUUCUCCGCAGACGACUCGCCCGA 3932. _H04
CTTTTTACCTTTCCTTTCTG GGGAGGACGAUGCGGCU UUUUACCUUUCCU UUCUGCAGACGACUCGCCCGA 3933. HQ4
TTTCTTTCATTTTTTTTTTCCG 6GGAGGAC6AUGCGGUU UCUUUCAUUUUUUU UUUCCGCAGACGACUCGCCCGA 3934. .H04
TTCACTTACTACTTTTTCTG GGGAGGACGAUGCGGUUCACUUACUACU UUUUCUGCAGACGACUCGCCCGA 3935. _HQ4
GTCTTCTTTTTCTTGTACCG GGGAGGACGAUGCGGGUCUUCUUUUUCUUGUACCGCAGACGACUCGCCCGA 3936. _H04
ccTTTTAcrrrnTTTCCG GGGAGGACGAUGCGGCCUUUUACUUUUUUU UCCGCAGACGACUCGCCCGA 3937. HQ4-
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ATTTCCAACTTTTCTTTCCG GGGAGGACGAUGCGGAUUUCCAACUUUUCUUUCCGCAGACGACUCGCCCGA 3938. _H04
TTTTA GCTCCTTTTTTTGTCCG GGGAGGACGAUGCGGUUU UAGCUCCUUUUUUUG UCCGCAGACGACUCGCCCGA 3939. _H04
TTTTC AATATCACTTTTCG C GGGAGGACGAUGCGGUUU UCAAUAUCACUUUUCCCCAGACGACUCGCCCGA 3942. _H04
ACTTTTTGTTACTTTTTACCC GGGAGGACGAUGCGGACUU UUUGU UACUUUUUACCCCAGACGACUCGCCCGA 3943. _HQ4
TTTATCTTTTACCTGTTCCC GGGAGGACGAUGCGGUUUAUCUUUUACCUGUUCCCCAGACGACUCGCCCGA 3944. H04
ATTCTCAATTTATTTCCCCG GGGAGGACGAUGCGGAUUCUCAAUU UAUUUCCCCGCAGACGACUCGCCCGA 3945. _H04
CTTTCTTTCTCTTTTTCCG GGGAGGACGAUGCGGCUUUCUU UCUCUUUUUCCGCAGACGACUCGCCCGA 3946.. .H04
TTTTCTACTTTCTTTTCCG G GG AGG ACGAUG CG 6U U U UCU ACU U U C U U U UCCG CAG ACG ACU CGCCCG A 3947. .H04
T7TTCTATTATTCCTTTCCG GGGAGGACGAUGCGGUUUUCUAUUAUUCCUUUCCGCAGACGACUCGCCCGA 3948. _H04
CTTTATTCTTTACTTTACTG GGGAGGACGAUGCGGCUUUAUUCUUUACUUUACUGCAGACGACUCGCCCGA 3949. .H04
TTTG GTGTTTTCCCGTG CCC GGGAGGACGAUGCGGU UUGGUGUUUUCCCGUGCCCCAGACGACUCGCCCGA 3950. .H04
TCTC ATG TTCCTC CTGTG CC 6GGA6GACGAUGCGGUCUCAUGUUCCUCCUGUGCCCAGACGACUCGCCCGA 3951. _H04
ο TCCTTTCGTCTTTTCGTCCC GGGAGGACGAUGCGGUCCUU UCGUCUUUUCGUCCCCAGACGACUCGCCCGA 3952. _H04
TCCTGTCGTCTTTGTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUUUGUG UCCCCAGACGACUCGCCCGA 3953. H04
TCCTTGTC GTCTTG CTTCCC GGGAGGACGAUGCGGUCCUUGUCGUCUUGCUUCCCCAGACGACUCGCCCGA 3955. _H04
ATTCTTCTATTTTCTCTCCG GGGAGGACGAUGCGGAUUCUUCUAUUUUCUCUCCGCAGACGACUCGCCCGA 3956. .H04
TCCTTGTCG TCTTG CCTC CC GGGAGGACGAUGCGGUCCUUGUCG UCUUGCCUCCCCAGACGACUCGCCCGA 3957, _HQ4
CCTTT CG TTCTACCCTCCC GGGAGGACGAUGCGGCCUUUCGUUCUACCCUCCCCAGACGACUCGCCCGA 3958. _H04
ATGCTTGGAGGAGGGAGGTG GGGAGGACGA'JGCGGAUGCUUGGAGGAGGGAGGUGCAGACGACUCGCCCGA 3959. .H04
TCCTGTCGTCTTTACGTCCC GGGAG-GACGAUGCGGUCCUGUC6UCUUUACGUCCCCAGACGACUCGCCCGA 3960. _H04
TGAGTCG TTCCCTTCG TTCC GGGAGGACGAUGCGGUGAG UCGUUCCCUUCGU UCCCAGACGA.CUCGCCCGA 3962. .H04
ATACTTCTACCTTTTCTCCG GGGAGGACGAUGCGGAUACUUCUACCUUUUCUCCGCAGACGACUCGCCCGA 3963. .H04
CGTTTCTCTGGTTCGTCCC GGGAGGACGAiJGCGGCG UUUCUCUGGUUCGUCCCCAGACGACUCGCCCGA 3964. _H04
TCCTGTCGTCCrrTCGTCCC GGGAGGACGAUGCGGUCCUGUCGUCCUUUCGUCCCCAGACGACUCGCCCGA 3965. _H04
GTTGGTGTAGGGGGTTCCG GGGAGGACGAUGCGGGUUGGUGUAGGGGGUUCCGCAGACGACUCGCCCGA 3966. _H04
TTTCCTC.TT6TT7TTGTCCC GGGAGGACGAUGCGGU UUCCUCUUGUUUU UGUCCCCAGACGACUCGCCCGA 3967 H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc
A CTTCTTACCTTTTTCTCCG GGGAGGACGAUGCGGACUUCUUACCUUUUUCUCCGCAGACGACUCGCCCGA 3968_H04
GGCTTCTCTCTTTACTTCCC GGGAGGACGAUGCGGGGCUUCUCUCUUUACUUCCCCAGACGACUCGCCCGA 3969_HQ4
CCTGTTTATTTTTTG CCCCC GGGAGGACGAUGCGGCCUGUUUAU UUUUUGCCCCCCAGACGACUCGCCCGA 397Q„H04
TCCAGCTTTCTTTTrCTCCG GGGAGGACGAUGCGGUCCAGCU UUCUUUUUCUCCGCAGACGACUCGCCCGA 3971. H04
TCTTTTG CTTTCTTTCCG GGGAGGACGAUGCGGUCUUUUGCUUUCUUUCCGCAGACGACUCGCCCGA 3973_H04
CTATTTCTTCTTG CCCTC C G GGGAGGACGAUGCGGCUAUUUCUUCUUGCCCUCCGCAGACGACUCGCCCGA 3974.H04
TTTG AAGGGAG ATG GGTCCT GGGAGGACGAUGCGGUUUGAAGGGAGAUGGGUCCUCAGACGACUCGCCCGA 3975_H04
CTTATGGAGTAGGGCGGTGC GGGAGGACGAUGCGGCUUAUGGAGUAGGGCGGUGCCAGACGACUCGCCCGA 3976_H04
ATGCTTGGAGTAGGGAGGGG G6GAGGACGAUGC6GAUGCUUGGAGUA6GGAGGGGCAGACGACUCGCCCGA 3977_H04
TCATCTTTGTCTTCTTTCCG GGGAGGACGAUGCGGUCAUCUUUG UCUUCUUUCCGCAGACGACUCGCCCGA 3978_H04
AGTTTCGTCTCTTTGTTCCG GGGAGGACGAUGCGGAGUU UCGUCUCUUUGUUCCGCAGACGACUCGCCCGA 3979_H04
TTCATTTCTTCTTCTCCG GGGAGGACGAUGCGGU UCAUUUCU UCUUCUCCGCAGACGACUCGCCCGA 3980_H04
crrTATccTTTTCTCCTccG GGGAGGACGAUGCGGCUUUAUCCUUUUCUCCUCCGCAGACGACUCGCCCGA 3981_H04
CCTTTTTCTTTTTCTACCG GGGAGGACGAUGCGGCCUUUUUCUUUUUCUACCGCAGACGACUCGCCCGA 3982._H04
T'CTATTCTTCTTTTCTC CGC GGGAGGACGAUGCGGUCUAUUCUUCUUUUCUCCGCCAGACGACUCGCCCGA 3983_H04
ATTTTTCTCTTTTG CTTGCCG GGGAGGACGAUGCGGAUUU UUCUCUUUUGCUUGCCGCAGACGACUCGCCCGA 39S4_H04
CTTTTTAACCACCTTTTCC G GGGAGGACGAUGCGGCUUU UUAACCACCUUUUCCGCAGACGACUCGCCCGA 39S5_H04
GTTAC'iTTCCTTTCCTACCG GGGAGGACGAUGCGGGUUACUiJUCCUUUCCUACCGCAGACGACUCGCCCGA 3986_H04
TAATTTCTATTTTCCGCTCCG GGGAGGACGAUGCGGUAAUUUCUAUUUUCCGCUCCGCAGACGACUCGCCCGA 39S7_H04
ATTCTTTCTCTTTTCACCG GGGAGGACGAUGCGGAUUCUUUCUCUUUUCACCGCAGACGACUCGCCCGA 398S„H04
TCTTTTTGTTTCTTATCCG GGGAGGACGAUGCGGUCUU UUUGUUUCUUAUCCGCAGACGACUCGCCCGA 3989_B04
CTTATGGAGTAGGGGGGTGC GGGAGGACGAUGCGGCUUAUGGAGUAGGGGGGUGCCAGACGACUCGCCCGA 3990_H04
GTCGAAGGGTGATGGGTCCG GGGAGGACGAUGCGGGUCGAAGGGUGAUGGG UCCGCAGACGACUCGCCCGA 3991JH04
TATGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUAUGGGAGUAGGGUG UUCCGCAGACGACUCGCCCGA 3992_H04
TTTG A AG G 6TTATG G GTCCC GGGAGGACGAUGCGGU'UUGAAGGGUUAUGGGUCCCCAGACGACUCGCCCGA 3993_H04
TTTCCTTGTTTTACTG CCCG GGGAGGACGAUGCGGUUUCCUUGUUUUACUGCCCGCAGACGACUCGCCCGA 3994.. H04
# in m
HTS read variable region RNA sequences selection Selected sequenc
TTATTTCATTTCCTTCTG CC GGGAGGACGAUGCGGUUAUUUCAUUUCCUUCUGCCCAGACGACUCGCCCGA 3995__H04
TCTATTATCCTTCTTTTCCG GGGAGGACGAUGCGGUCUAUUAUCCUUCUUU UCCGGAGACGACUCGCCCGA 3996_HD
AATCTACCTTTTTTTTCCTCCG GGGAGGACGAUGCGGAAUCUACCUUUUUUUUCCUCCGCAGACGACUCGCCCGA 3997_H04
TTCGTTTAACTCTT TTTCCG GGGAGGACGAUGCGGUUCGUUUAACUCU UUUUCCGCAGACGACUCGCCCGA 3998_H04
TTTC ATATG CTTTTTCGCTG GGGAGGACGAUGCGGU UUCAUAUGCUUUUUCCCUGCAGACGACUCGCCCGA 3999_H0
ATTCGCTCATCTTTTTG CCG GGGAGGACGAUGCGGAUUCGCUCAUCU UUUUGCCGCAGACGACUCGCCCGA 4000_H04
TTCC G CTATACTTTCTTC C G GGGAGGACGAUGCGGUUCCGCUAUACUUUCUUCCGCAGACGACUCGCCCGA 4001_H04
TGTTAG GTC I I 1 1 f I iTTCCG GGGAGGACGAUGCGGUGUUAGGUCULJUUUUUUUCCGCAGACGACUCGCCCGA 4OO2_H04
TTTATTTCTCCTGTTTTCCC GGGAGGACGAUGCGG UUUAUUUCUCCUGUU UUCCCCAGACGACUCGCCCGA 4003_H04
AATGCTTTTTCTTCTATCCG GGGAGGACGAUGCGGAAUCCUUUU'JCUUCUAUCCGCAGACGACUCGCCCGA 4004_HQ4
AATTTTTC6CTTTGTTACCG GGGAGGACGAUGCGGAAUUUUUCGCUUUGU UACCGCAGACGACUCGCCCGA 40Q5_ H04
TCTTATTTATGCCTTATCCG GGGAGGACGAUGCGGUCUUAUU UAUGCCUUAUCCGCAGACGACUCGCCCGA 40Q6_H04
TTCGCTACATTTTTTATCCC. GGGAGGACGAUGCGGU UCGCUACAU UUUUUAUCCCCAGACGACUCGCCCGA 4007_H04
TACATTCTTATTTTCTTCG C GGGAGGACGAUGCGGUACAUUCUUAUUUUCUUCGCCAGACGACUCGCCCGA 4008... H04
TCTTTAG CTTTTCCCATC CG GGGAGGACGAUGCGGUCUUUAGCUUUUCCCAUCCGCAGACGACUCGCCCGA 4009_H04
TTTCCTTTTATTTTC C CCAC GGGAGGACGAUGCGGUUUCCUU UUAUUUUCCCCACCAGACGACUCGCCCGA 4010_H04
ATATCACTTTCCTTTCG CCG GGGAGGACGAUGCGGAUAUCACUUUCCUUUCGCCGCAGACGACUCGCCCGA 4011_H04
TTTCTCTTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUCUCU UUUCCUUUG UCCGCAGACGACUCGCCCGA 4012_H04
TCCCATTTTGTCTTTTTCCG GGGAGGACGAUGCGGUCCCAUUUUG UCUU UU UCCGCAGACGACUCGCCCGA 4013_H04
TTTTTT GTTCCATTTTATCCG GGGAGGACGAUGCGGUUUUUUGUUCCAUUU UAUCCGCAGACGACUCGCCCGA 4014_H04
ATA TTTTG TTTTTTTG CCC GGGAGGACGAUGCGGAUAUU UUGUUUUUUUGCCCCAGACGACUCGCCCGA 4015„.Η04
CTTATATTf C ACTG TTTCCG GGGAGGACGAUGCGGCU UAUAUUUCACUGUUUCCGCAGACGACUCGCCCGA 40i6_H04
TTTGTTTTTCTCTTTTTCCC GGGAGGACGAUGCGGUUUGUUUUUCUCUUUUUCCCCAGACGACUCGCCCGA 4Q17_H04
CC TTTGTTTTTTTTCCTCCG GGGAGGACGAUGCGGCCAUUUGUUUUUU UUCCUCCGCAGACGACUCGCCCGA 4018_H04
6AACTCTTTTTTTTTTCGCCC GGGAGGACGAUGCGGGAACUCUUUUUUUUUUCGCCCCAGACGACUCGCCCGA 4021_H04
CCATTTGTTTTTTTCTTCCG GGGAGGACGAUGCGGCCAUU UGUUUUUUUCUUCCGCAGACGACUCGCCCGA 4022_H04
U in H4
HTS read variable region NA sequences action selection Selected sequenc TIG A AG 6 GTG TTGG 6 TCCG GGGAGGACGAUGCGGUUGAAGGGUGUUGGGUCC6CAGACGACUCGCCCGA 4026_H04
TTCACTAACATTTCTTTCCG GGGAGGACGAUGCGGUUCACUAACAUUUCUUUCCGCAGACGACUCGCCCGA 4027_H04
CTTTTAATGATGCTTTTCCG GGGAGGACGAUGCGGCUUU UAAUGAUGCUUUUCCGCAGACGACUCGCCCGA 4028_HQ4
ATTTTTTGTATTCTTTTGCCC GGGAGGACGAUGCGGAUU UUUUG UAUUCU U UUGCCCCAGACGACUCGCCCGA 4029_HQ4
CGTTGTTAATGTTTTTTGCC GGGAGGACGAUGCGGCGUUGUUAAUGU UUUUUGCCCAGACGACUCGCCCGA 4030_HQ4
CTTTCTTC G TTTCTTTCCCG GGGAGGACGAUGCGGCUUUCUUCGUU UCUUUCCCGCAGACGACUCGCCCGA 4032_H04
ATATTTCATTCTTTCTACCC GGGAGGACGAUGCGGAUAUUUCAUUCUUUCUACCCCAGACGACUCGCCCGA 4033_H04
ATTTTCAT677TCGTTCCCG GGGAGGACGAUGCGGAUUUUCAUG UUUCGU UCCCGCAGACGACUCGCCCGA 4034_H04
GTTTTTTTCTATTG TTTCCG GGGAGGACGAUGCGGG UUUUUUUCUAUUGUUUCCGCAGACGACUCGCCCGA 4035_H04
CTTTTTCTATTCCCATACCG GGGAGGACGAUGCGGCU UUUUCUAUUCCCAUACCGCAGACGACUCGCCCGA 4036_H04
TTTTTGTCTCTTATTTCCTG GGGAGGACGAUGCGGU UUU UGUCUCUUAUU UCCUGCAGACGACUCGCCCGA 4037_H04
TTTATTCATTTTCTTGTCG C GGGAGGACGAUGCGGUUUAUUCAUUUUCUUGUCGCCAGACGACUCGCCCGA 4038„H04
CACTTTTTTATCTTTACCCCC GG6A6GACGAUGCGGCACUUUUUUAUCUUUACCCCCCAGACGACUCGCCCGA 4039_H04
TTTTTCTTCTTTTTTCC G GGGAGGACGAUGCGGUU.UUUCUUCUUU UUUCCGCAGACGACUCGCCCGA 4O4O_H04
ATCTTG GTTTTT ATTTCC C G GGGAGGACGAUGCGGAUCUUGGUU UUUAUU UCCCGCAGACGACUCGCCCGA 4041_ H04
CATCTACTCTTTACTCTCCG GGGAGGACGAUGCGGCAUCUACUCUUUACUCUCCGCAGACGACUCGCCCGA 4042_H04
ACTTCGTTTCACTTTCACCG GGGAGGACGAUGCGGACUUCGUUUCACUUUCACCGCAGACGACUCGCCCGA 4G43_H04
TTTTCTACCAGCmCTCCG GGGAGGACGAUGCGGUUUUCUACCAGCUUUCUCCGCAGACGACUCGCCCGA 4044_H04
TTCACTTCTTTCTTTTTCG C GGGAGGACGAUGCGGUUCACUUCUUUCUUUU UCGCCAGACGACUCGCCCGA 4045_H04
CATTTTCTACTTTTTTTCCG GGGAGGACGAUGCGGCAUU UUCUACUU UUUUUCCGCAGACGACUCGCCCGA 4046_H04
CTCTTTGCTTCTTTATCCCG GGGAGGACGAUGCGGCUCUUUGCUUCUUUAUCCCGCAGACGACUCGCCCGA 4047_H04
TATTTCACTG I ΤΊ 'Π Τ i TGCTG GGGAGGACGAUGCGGUAUU'UCACUGUUUUUU UUGCUGCAGACGACUCGCCCGA 4048 H04
TTnTCGTGTTTTI'ACCCCG GGGAGGACGAUGCGGU UUU UCGUGUUUUUACCCCGCAGACGACUCGCCCGA 4049_H04
TTATTGTTTCATTTTGTGTG GGGAGGACGAUGCGGU UAU UGUUUCAUUUUGUGiJGCAGACGACUCGCCCGA 405G_H04
CTTATTTATCTTTCTCTCCG GGGAGGACGAUGCGGCU UAUUUAUCUUUCUCUCCGCAGACGACUCGCCCGA 4051_H04
TTTGCTTTTTTTTTCCTTG CC GGGAGGACGAUGCGGUUUGCUUUUUUUUUCCUUGCCCAGACGACUCGCGCGA 4052 H04
c
01 3 S3"
EU
Figure imgf000285_0001
X
cr
Figure imgf000285_0002
# in H4
HTS read variable region RNA sequences selection selection Selected sequenc TTTTA ACTTTTTCCTTTG C A GGGAGGACGAUGCGGU UUU.AACUUUUUCCUU UGCACAGACGACUCGCCCGA 4081_H04
CCTTTTTATTTCATTTTCCG GGGAGGACGAUGCGGCCUU UUUAUUUCAUUU UCCGCAGACGACUCGCCCGA 4Q82_H04
TTTATTTTCGATTTTCTGCC GGGAGGACGAUGCGGUUUAUUUUCGAUUUUCUGCCCAGACGACUCGCCCGA 4083_H04
TCCTTTATCTTGTT'T'l'TCCG GGGAGGACGAUGCGGUCCUUUAUCUUGUUUUUCCGCAGACGACUCGCCCGA 4084_H04
TTTTTCTAATT AC G TTCCG GGGAGGACGAUGCGGUUU UUCUAAUUACGUUCCGCAGACGACUCGCCCGA 408S_H04
TTATTCT I I ! I i I I GTGTC GGGAGGACGAUGCGGUUAUUCUCUUUU UUUGUGUCCAGACGACUCGCCCGA 40S6__H04
CTTTTTT G GTTTTCTTT CCG GGGAGGACGAUGCGGCUUUUUUGGUUUUCUUUCCGCAGACGACUCGCCCGA 4087_H04
TTTGGGTTCTT 1 1 M 1 1 CTG GGGAGGACGAUGCGGUUUGGGUUCUUUUUUUUCUGCAGACGACUCGCCCGA 4088_ HQ4
GTTTCG CCTG TTTT CTTC C G GGGAGGACGAUGCGGG UUUCGCCUGUUUUCU UCCGCAGACGACUCGCCCGA 4089_H04
GTTTTATTTATTCGTTTCCG GGGAGGACGAUGCGGGUUU UAUUUAUUCGU UUCCGCAGACGACUCGCCCGA 4090„H04
TACT ATTCTTCTTTTTG CCG GGGAGGACGAUGCGGUACUAUUCUUCUUUUUGCCGCAGACGACUCGCCCGA 4091__H04
TTTCTTTACTTTCTTTCCG GGGAGGACGAUGCGGUULICUUUACUUUCU UUCC6CAGACGACUCGCCCGA 4092_H04
CCTTTTAACGTTTCGCTCCG GGGAGGACGAUGCGGCCUUUUAACGUUUCGCUeCGCAGACGACUCGCCCGA 4095_ H04
TTTTAGCT TTTTTTTGTCCG GGGAGGACGAUGCGGUU UUAGCU UUUUUUU UGUCCGCAGACGACUCGCCCGA 4G96_H04
rrrTTACCTTTTTCGTTCCG GGGAGGACGAUGCGGUU UUUACCUUU UUCGUUCCGCAGACGACUCGCCCGA 409 _H04
TCTTTTTTTTCTTCTA.CCCCCG GGGAGGACGAUGCGGUCUU UUUUU UCUUCUACCCCCGCAGACGACUCGCCCGA 4098_H04
TCTTTTAACGTGTTTTCCCG G6GAGGACGAUGCGG UCUUUUAACGUGUUUUCCCGCAGACGACUCGCCCGA 4099_H04
GTTTCTTCTTTATTACTCCC GGGAGGACGAUGCGGGUUUCUUCUUUAUUACUCCCCAGACGACUCGCCCGA 4100JHG4
TTCTrTATTTTTTT'CCCTCCT GGGAGGACGAUGCGGUUCUUUAUUUUUU UCCCUCCUCAGACGACUCGCCCGA 4101JH04
TTTGG 6GTTTTCCAGTGCCC GGGAGGAC6AUGCGGUUUGGGGUUUUCCAGUGCCCCAGACGACUCGCCCGA 4102_H04
CCCTGTCGTCTTTGCGTCCC GGGAGGACGAUGCGGCCCUGUCGUCUUUGCGUCCCCAGACGACUCGCCCGA 41Q3_H04
TCCTTATCG TCTTG CGTCCC GGGAGGACGAUGCGGUCCUUALJCGUCUUGCG'JCCCCAGACGACUCGCCCGA 4104_H04
TCGTGACGTCTGTGCGTCCC GGGAGGACGAUGCGGUCCUGACGUCUGUGCG UCCCCAGACGACUCGCCCGA 4105_H04
CTTTGCTCTTTTTCTCCG GGGAGGACGAUGCGGCUUUGCUCUUUUUCUCCGCAGACGACUCGCCCGA 4106_H04
TGAGTCGTTCTGTTCGTCCC GGGAGGACGAUGCGGUGAGUCGUUCUCUUCGUCCCCAGACGACUCGCCCGA 4107_H04
TTTATTTCTTCCACG TTCCG GGGAGGACGAUGCGG UU UAUU U CU UCCACG UUCC6CAGACG ACU C6CCCGA 4108 H04
# m H
HTS read variable region NA sequences selection selection Selected sequenc
ATCTTTATTTTTTCCCTCCG GGGAGGACGAUGCGGAUCUUUAUUUUUUCCCUCCGCAGACGACUCGCCCGA 4109 H04
TACCTTATCCTG CAG GTGCC GGGAGGACGAUGCGGUACCUUAUCCUGCAGGUCCCCAGACGACUCGCCCGA 411GJHG4
GTCCTGTCGTCTGTTTGTCCC 6GGAGGACGAUGCGGGUCCUGUCGUCUGUUUGUCCCCAGACGACUCGCCCGA 4111„H04
TGCTG G CTTG TTGTTG TCC C GGGAGGACGAUGCGGUCCUGGCUUGUUGUU6UCCCCAGACGACUCGCCCGA 4112_H04
TTTACTTTCCTCTTGCCG GGGAGGACGAUGCGGU UUACUUUCCUCUUGCCGCAGACGACUCGCCCGA 4113_ H04
TTTG GGAG AAG GGGGTTCCG GGGAGGACGAUGCGGU UUGGGA6AAGGGGG UUCCGCAGACGACUCGCCCGA 114_HQ4
TTACCCTTACTTTTGCTCCG GGGAGGACGAUGC6GU UACCCUUACUUUUGCUCCGCAGAC6ACUCGCCCGA 4115_H04
ATTTTTCCTGTTTT7GCCG GGGAGGACGAUGCGGAUUU UUCCUGUUU UUGCCGCAGACGACUCGCCCGA 4116_H04
ATGCTTGGAGTATGGAGGTG GGGAGGACGAUGCGGAUGCU UGGAGUAUGGAGGUGCA6AC6ACUCGCCCGA 4117_H04
TTTG AAG GGTG ATGTTTCCG GGGAGGACGAUGCCGUUUGAAGGGUGAUGU UUCCGCAGACGACUCGCCCGA 4118_H04
TCCTGTCGTCTGGTTGTCCC GGGAGGACGAUGCGGUCCUGUCGUCUGGUUGUCCCCAGACGACUCGCCCGA 4U9_H04
ATCTTTTCTTTCCTCTGCCG GGGAGGACGAUGCGGAUCU UUUCUUUCCUCUGCCGCAGACGACUCGCCCGA 4120_H04
TTTTTG CTCTTTTCCCTCCG GGGAGGACGAUGCGGUUUUUGCUCUUUUCCCUCCGCAGACGACUCGCCCGA 4121_ H04
TCTTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGUCUU UUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 4122_H04
TCTTT6TTATTTTCATCCG GGGAGGACGAUGCGGUCUU UGU UAUUUUCAUCCGCAGACGACUCGCCCGA 4125_H04
TGTTTTTTACTCTTTTGCGC GGGAGGACGAUGCGGUGUU UU UUACUCUUUUGCGCCAGACGACUCGCCCGA 4126_HQ4
ACCATTCTTATCTCCTTCCG GGGAGGACGAUGCGGACCAUUCUUAUCUCCU UCCGCAGACGACUCGCCCGA 4127„H04
AATTACTTACTTTTTACCCC GGGAGGACGAUGCGGAAUUACUUACUUUU UACCCCCAGACGACUCGCCCGA 4128_H04
ATTCTTTTG 1' I ! 1 1 ! I !CCCG GGGAGGACGAUGCGGAUUCUUU UGU UUUUUUUCCCGCAGACGACUCGCCCGA 4129..H04
CCTTTCTTAT CTACCTCCG GGGAGGACGAUGCGGCCUUUCUUAUUCUACCUCCGCAGACGACUCGCCCGA 4130_H04
GTATTG CTTTCTTTTTGCCC GGGAGGACGAUGCGGGUAUyGCU UUCUUUUUGCCCCAGACGACUCGCCCGA 4l3l_H04
TACTTTCTTTCCACCTTCCG GGGAGGACGAUGCGGUACUU UCUUUCCACCUUCCGCAGACGACUCGCCCGA 4132_ H04
ATTTCTCTTTTTTTCGTCCCG GGGAGGACGAUGCGGAU UUCUCUUUUUUUCGUCCCGCAGACGACUCGCCCGA 4133_HG4
GTTrATAGCCTCTTTTTCCG GGGAGGACGAUGCGGGUUUAUAGCCUCUUU UUCCGCAGACGACUCGCCCGA 4134_H04
TTTCCTTTTTTCCCATCCG GGGAGGACGAUGCGGUU UCCUUUUUUCCCAUCCGCAGACGACUCGCCCGA 4135_H04
GCCTTTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUUUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 4137 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CTCCTTGTTGCTTTTTCTCCG GGGAGGACGAU6CGGCUCCUUGUUGCUUUUUCUCCGCAGAC6ACUCGCCCGA 4133. _H04
TTATCACTTTTCCTTGTCCC GGGAGGACGAUGCGGUUAUCACUUUUCCUUGUCCCCAGACGACUCGCCCGA 4139. _H04
CTTTCACTTTTTTTCCTCTCCG GGGAGGACGAUGCGGCUUUCACUUUUUUUCCUCUCCGCAGACGACUCGCCCGA 4140. H04
ATTACTCTTCTCTTTGCCG GGGAGGACGAUGCGGAUUACUCUUCUCUUUGCCGCAGACGACUCGCCCGA 4141_ _H04
Cr.TTTTACCTTTTTTCTCCG GGGAGGACGAUGCGGCCUUUUACCUUUUUUCUCCGCAGACGACUCGCCCGA 4142, -H04
TTTCTTTTTA'fTCCTGTCCG GGGAGGACGAUGCGGUUUCUUUUUAUUCCUGUCCGCAGACGACUCGCCCGA 4143. H04
ATTCACTTCTTTTCTTTCCG GGGAGGACGAUGCGGAUUCACUUCUUUUCUU UCCGCAGACCACUCGCCCGA 4145, .H04
TCTTTTC ATG TTTTCTCTCCG GGGAGGACGAUGCGGUCUU UUCAUGUUUUCUCUCCGCAGACGACUCGCCCGA 4146. H0
GTTCACTCTTTCCATTCCCC GGGAGGACGAUGCGGGUUCACUCUUUCCAUUCCCCCAGACGACUCGCCCGA 4147_ .H04
AG TTTTTTG TCTTTTTTCCC C GGGAGGACGAUGCGGAGUU UUUUGUCUUUUUUCCCCCAGACGACUCGCCCGA 4148. _H04
CTTACCGTTTTCTTCTTCCG GGGAGGACGAUGCGGCUUACCGUUUUCUUCUUCCGCAGACGACUCGCCCGA 4149. ,H04
CTCTATTATCCTCTTCTCCG GGGAGGACGAUGCGGCUCUAUUAUCCUCUUCUCCGCAGACGACUCGCCCGA 4150. .H04
GTTTGTTTCTTTAGTTTCCC GGGAGGACGAUGCGGGUU UGU UUCUUUAGUUUCCCCAGACGACUCGCCCGA 4151. H04
CTTTTTTTGTTCTTTCATCCG GGGAGGACGAUGCGGCUUU UUUUGUUCUUUCAUCCGCAGACGACUCGCCCGA 4152. _H04
TATTGCTTGTATTTCCTCCG GGGAGGACGAUGCGGUAUUGCUUGUAUUUCCUCCGCAGACGACUCGCCCGA 4153. O
TCTACCTTTACTTCTTCCCG GGGAGGACGAUGCGGUCUACCUUUACUUCUUCCCGCAGACGACUCGCCCGA 4154. H04
GCTTTTCGTTCTGCCTCCC GGGAGGACGAUGCGGGCUUUUCGUUCUGCCUCCCCAGACGACUCGCCCGA 4155. _H04
GCCTCTCGTTCTGCTCTCCC GGGAGGACGAUGCGGGCCUCUCGUUCUGCUCUCCCCAGACGACUCGCCCGA 4156. H04
C TTCTTTTATCTCTCTTCCG GGGAGGACGAUGCGGCU UCUUUUAUCUCUCUUCCGCAGACGACUCGCCCGA 4157. _H04
CATTTCACCTTA CTTTTCCG GGGAGGACGAUGCGGCAUUUCACCUUACUUUUCCGCAGACGACUCGCCCGA 4158. H04
ATCTTCTTATTTTCTG CCG GGGAGGACGAUGCGGAUCU UCUUAUUUUCUGCCGCAGACGACUCGCCCGA 4159. H04
CTTTCATTG TTCTTTTG CCC GGGAGGACGAUGCGGCUUUCAU UGUUCUUUUGCCCCAGACGACUCGCCCGA 416.0. _H04
TTTGTATTCCCCTTTTTTCCG GGGAGGACGAUGCGGUUUGUAUUCCCCUUUU UL!CCGCAGACGACUCGCCCGA 4164. .H04
TTTTCTTTTCCTTTGTCCG GGGAGGACGAUGCGGUUUUCUUUUCCUUUGUCCGCAGACGACUCGCCCGA 4165, _H04
GTtGGAGTAGGGGGGTTCCG GGGAGGACGAUGCGGGUUGGAGUAGGGGGG UUCCGCAGACGACUCGCCCGA 4166. . H04
ATTTTCTCTTTTTTTTTG CCG GGGAGGACGAUGCGGAU UUUCUCU UUUUUUUUGCCGCAGACGACUCGCCCGA 4167 H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc
TCACTTTTCTTTTCTTACCC GGGAGGACGAUGCGGUCACU UUUCUUUUCUUACeCCAGACGACUCGCCCGA 4168 H04
TTTGT AGGGTGATGGGACCG GGGAGGACGAUGCGGU UUGUAGGGUGAUGGGACCGCAGACGACUCGCCCGA 4169_H04
ATATCCTCTTTTTTCTGCCG GGGAGGACGAUGCGGAUAUCCUCUU UUUUCUGCCGCAGACGACUCGCCCGA 417Q_H04
TTATTCCTTTTT TTG CCGC GGGAGGACGAUGCGGUUAU UCCUU UUUCUUGCCGCCAGACGACUCGCCCGA 171_H04
G7TGGTGTAGG6GTGTTCCG GGGAGGACGAUGCGGGUUGGUGUAGGGGUGUUCCGCAGACGACUCGCCCGA 4172 JH04
TTTGAAGGGGTATGGGTCCG GGGAG6ACGAUGCGGUUUGAAGGGGUAUGGGUCCGCAGACGACUCGCCCGA 4173„H04
GTTTTTGTTG 6 1 1 I I ! I I CCG GGGAGGACGAUGCGGGUUUUUGUUGGUUUUUUUCCGCAGACGACUCGCCCGA 4174_H04
TTTTATATCTTCCTTTTG CC GGGAGGACGAUGCGGUUUUAUAUCUUCCUUUUGCCCAGACGACUCGCCCGA 4i77_H04
CCTTTTTTATTCCTTTTGCCG GGGAGGACGAUGCGGCCUUU UUUAUUCCU UUUGCCGCAGACGACUCGCCCGA 4178_H04
TTTTG AAGGGTGATGGGCCCG GGGAGGACGAUGCGGU UUUGAAGGGUGAUGGGCCCGCAGACGACUCGCCCGA 4179_H04
Ι Π Ι Ι Ί I'CCTTTCACTATCCG GGGAGGACGAUGCGGU UUU UUUCCUUUCACUAUCCGCAGACGACUCGCCCGA 4180_H04
TCATTCTTTTCTTCTTACGC GGGAGGACGAUGCGGUCAU UCUUUUCUUCUUACGCCAGACGACUCGCCCGA 4181_H04
rCTTATACTGATGGCCGCGT GGGAGGACGAUGCGGUCUUAiJACUGAUGGCCGCGUCAGACGACUCGCCCGA 4184_H04
TCTTTATTTG TACTCTCCTG GGGAGGACGAUGCGGUCUU UAUUUGUACUCUCCUGCAGACGACUCGCCCGA 4186_H04
TTCGTTTTTG CATTTCTCCG GGGAGGACGAUGCGGUUCGUUUUUGCAUUUCUCCGCAGACGACUCGCCCGA 4187_H04
TGTTGTTTTCGTGTTTTCCG GGGAGGACGAUGCGG UGUUGU UUUCGUGUUUUCCGCAGACGACUCGCCCGA 4188_H04
ATTCCACTTTTTTTTTCGCCG GGGAGGACGAUGCGGAU UCCACUUUUUUUUUCGCCGCAGACGACUCGCCCGA 41'89_H04
AATTTCTTTTCTACCCGCCG GGGAGGACGAUGCGGAAUUUCU UUUCUACCCGCCGCAGACGACUC6CCCGA 4190_H04
ACTGTTT!TATCATTTTGCC GGGAGGACGAUGCGGACUGU UUUUAUCAU UUUGCCCAGACGACUCGCCCGA 4191_H04
TATTTTAATTCTCCTTTGCC GGGAGGACGAUGCGGUAUU UUAAUUCUCCUUUGCCCAGACGACUCGCCCGA 4192_H04
CTCT I I 1 1 !' i ] GTTTCCCCG GGGAGGACGAUGCGGCUCUU UUU UUUGUUUCCCCGCAGACGACUCGCCCGA 4193_H04
TGCTTTG GTTTTTTTTCCG GGGAGGACGAUGCGGUGCUUUGGUUUUUUUUCCGCAGACGACUCGCCCGA 4194..HG4
TGTG CTTGATTATTTTTCCG GGGAGGACGAUGCGGUG UGCUUGAUUAUUUUUCCGCAGACGACUCGCCCGA 4195_H04
TTTTTGTTT1TTTTACGCG GGGAGGACGAUGCGGUU UUUGUUUUU UUUACCCGCAGACGACUCGCCCGA 4196„H04
CTTTTCTTCTTTTCCTTCCG GGGAGGACGAUGCGGCUUUUCUUCUUUUCCLfUCCGCAGACGACUCGCCCGA 4197_H04
TTTTTT ATATCATGTTC CCG GGGAGGACGAUGCGGUUUUUUAUAUCAUGUUCCCGCAGACGACUCGCCCGA 4198 H04
# in H4
ΗΤ5 read variable region RNA sequences # in H3 selection selection Selected sequenc CTTTTCATT CTCTTC6 TTG C GGGAGGACGAUGCGGCUUU UCAUUCUCUUCGU UGCCAGACGACUCGCCCG 199JH04
CCTGTTTTCTTGTTATTCCG GGGAGGACGAUGCGGCCUG UUUUCUUGUUAU UCCGCAGACGACUCGCCCGA 42QO_H04
CTTATTCTTACTTTACTCCG GGGAGGACGAUGCGGCUUAUUCUUACU UUACUCCGCAGACGACUCGCCCGA 4201_H04
GCCTTATCTTTTTTACTCCG GGGAGGACGAUGCGGGCCUUAUCUUUUUUACUCCGCAGACGACUCGCCCGA 4202_H04
CTTTGTTTTATTCTTGTTGC GGGAGGACGAUGCGGCU UUGU UUUAUUCUUGUUGCCAGACGACUCGCCCGA 4203_H04
CTTTGTCTTTTATTCTCCCG GGGAGGACGAUGCGGCUUUGUCUUUUAU UCUCCCGCAGACGACUCGCCCGA 4204„H04
TCTCTTTAGCATTTTTTCCG GGGAGGACGAUGCGGUCUCUUUAGCAUUUUU UCCGCAGACGACUCGCCCGA 4205_HQ4
TGTCTA7TTATTTCCTTCCG GGGAGGACGAUGCGGUGUCUAUUUAUUUCCUUCCGCAGACGAGUCGCCCGA 4206_.HQ4
ΪΤΓΠ Απ τ6Τ€π6Τ< 0 GGGAGGACGAUGCGGUUUUCAUCCUGUCUUGUCCGCAGACGACUCGCCCGA 4207_H04
GACTTCTTATCTTTTCTCCC GGGAGGACGAUGCGGGACUUCUUAUCUUUUCUCCCCAGACGACUCGCCCGA 4208_H04
TTTTCTATTTTAACTCTCCC GGGAGGACGAUGCGGU UUUCUAUU UUAACUCUCCCCAGACGACUCGCCCGA 4209_H04
TACCATTTCTTTCTCTTGTG GGGAGGACGAUGCGGUACCAUUUCUUUCUCUUGU6CAGACGACUCGCCCGA 421Q_H04
TATTTTCTTTTCTGTGTCCC GGGAGGACGAUGCGGUAUUUUCUUUUCUGUGUCCCCAGACGACUCGCCCGA 4211J-i04
TTTTCTTG CTTTTTCCG CTA GGGAGGACGAUGCGGUUUUCUUGCUUUU UCCGCUACAGACGACUCGCCCGA 4212„H04
GTTTTTTTACTTATGTTGCCG GGGAGGACGAUGCGGGUUU UUU UACUUAUGUUGCCGCAGACGACUCGCCCGA 4213_ H04
TTCTTG T ATTAATTTTTCCG GGGAGGACGAUGCGGUUCUUGUAUUAAUUUU UCCGCAGACGACUCGCCCGA 4214_H04
TCTTTTCATGTTTATTCCC GGGAGGACGAUGCGGUCUUUUCAUGU UUAUUCCCCAGACGACUCGCCCGA 4215_H04
CATTCCTTTATTTCTTCGCC GGGAGGACGAUGCGGCAUUCCUU UAUUUCU UCGCCCAGACGACUCGCCCGA 4216_H04
CATTCTTCTGTTTTTCCTTCGC GGGAGGACGAUGCGGCAUUCUUCUGUUUUUCCUUCGCCAGACGACUCGCCCGA 4217_H04
CGAATGGAGGAGGAGGATGC GGGAGGACGAUGCGGCGAAUGGAGGAGGAGGAUGCCAGACGACUCGCCCGA 4218JH04
ATTTTCATATCTTTTCCCGC GGGAGGACGAUGCGGAUUUUCAUAUCUUUUCCCGCCAGACGACUCGCCCGA 4219_H04
TTTTTGTTTTGTTTTTGCTG GGGAGGACGAUGCGGUUUU UG UUUUGUUUUUGCUGCAGACGACUCGCCCGA 4220„H04
CTTTTCTTTCATTTTCC C GG GGGAGGACGAUGCGGCUUUUCUUUCAUUUUCCC6GCAGACGACUCGCCCGA 4221_H04
TTTGCTATTCATTTTTCCCC GGGAGGACGAUGCGGUUUGCUAUUCAUUUUUCCCCCAGACGACUCGCCCGA 4222.. H04
TTTG G G AGTAGG GTGTTCG G GGGAGGACGAUGCGGUUUGGGAGUAGGG UGUUCGGCAGACGACUCGCCCGA 4223_ H04
GTTG GTGT AG GGGG G GTCCG GGGAGGACGAUGCGGGU UGGUGUAGGGGGGGUCCGCAGACGACUCGCCCGA 4225 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TACTTTTTCTTCCTTTTCCA GGGAGGACGAUGCGGUACUUU UUCUUCCUUUUCCACAGACGACUCGCCCGA 4226. _H04
TTCGTTTArrAATTTCTCCG GGGAGGACGAUGCGGUUCG UUUAU UAAUUUCUCCGCAGACGACUCGCCC6A 4227. .H04
CTTTTAAACTCCTTTTCCCG GGGAGGACGAU6CGGCUUU UAAACUCCUUUUCCCGCAGACGACUCGCCCGA 4228. .H04
TTTTTTAGATAGTTTTTGTG G6GAGGACGAUGCG6U UU UUUAGAUAGUUUUUGUGCAGACGACUCGCCCGA 4229. _HG4
TTTCCACTACATTATTTCCG GGGAGGACGAUGCGGU UUCCACUACAUUAUUUCCGCAGACGACUCGCCCGA 4230 H04
TTTTCTCATGCCTTATCCG 6GGAGGACGAUGCGGCU UUUCUCAUGCCUUAUCCGCAGACGACUCGCCCGA 4231. _H04
TTTG CTCTCTATTTTTTTCCG GGGAGGACGAUGCGGU UUGCUCUCUAUUUUUUUCCGCAGACGACUCGCCCGA 4232. „HG4
TCTCTGTCATTCTATTTCC6 GGGAGGACGAUGCGGUCUCUGUCAUUCUAUUUCCGCAGACGACUCGCCCGA 4233. .H04
ATTCTACTATTA CTCCACCG GGGAGGACGAUGCGGAUUCUACUAUUACUCCACCGCAGACGACUCGCCC6A 4234. .H04
TTTGATATTTCTCTCCTCTG GGGAGGACGAUGCGGUUUCAUAUUUCUCUCCUCUGCAGACGACUCGCCCGA 4235. _KQ4
CACTCACTTTTTTTCTTCG C GGGAGGACGAUGCGGCACUCACUUUUU UUCUUCGCCAGACGACUCGCCCGA 4236. _H04
GCCTCTCCTTTTTCTTCCCG GGGAGGACGAUGCGGGCCUCUCCU UUUUCUUCCCGCAGACGACUCGCCCGA 4237. .H04
TTTAC ATTTTCTCACCTG CCC GGGAGGACGAUGCGGUUUACAUUUUCUCACCUGCCCCAGACGACUCGCCCGA
ο 4239. .H04
CTTTCTTTGTTTCCTTCCG G6GAGGACGAUGCGGCUUUCUUUGUUUCCUUCCGCAGACGACUCGCCCGA 4241. _H04
ATTTTTTG CCGTGTTCTCCG GGGAGGACGAUGCGGAUUUUU UGCCGUG UUCUCCGCAGACGACUCGCCCGA 4242. _HQ4
ATTTTTCTCTTTT TCTG CCG GGGAGGACGAUGCGGAUUUUUCUCUUUUUUCUGCCGCAGACGACUCGCCCGA 4243. .H04
TTTTCTCTTTTCCTTTTCCG GGGAGGACGAUGCGGUUUUCUCUUUUCCUUUUCCGCAGACGACUCGCCCGA 4245. .H04
TTTCTTTTTTTGTCTTG CTCCG GGGAGGACGAUGC6GU UUCUUUUUUUGUCUUGCUCCGCAGAC6ACUCGCCCGA 4246. H04
TTTCTTTTGCTTTTTCTCCG GGGACCACGAUGCGGUUUCUUUUGCUUUUUCUCCGCAGACGACUCGCCCGA 4247. .H04
TTTTTTGTCTTTTGCTCCG GGGAGGACGAUGCGGUUUU UUGUCUUUUGCUCCGCAGACGACUCGCCCGA 4248. _H04
TTTATTTTTCTCGTTG TTGTG GGGAGGAC6AUGCGGUU UAUUU UUCUCGUUGUUGUGCAGACGACUCGCCCGA 4249. _H04
TTCACTTTCTTTTCTCTCCG GG'GAGGACGAUGCGGUUCACUUUCUUUUCUCUCCGCAGACGACUCGCCCGA 4250. .H04
TCTTTTCATGTTTCTTCCG GGGAGGACGAUGCGGUCUUUUCAUGUUUCUUCCGCAGACGACUCGCCCGA 4254. „HG4
TTATCTAACTCTTTTCCCCG GGGAGGACGAUGCGG UUAUCUAACUCUUUUCCCCGCAGACGACUCGCCCGA 4255. _H04
TTG TTACTG ATTTTATTCC G GGGAGGACGAUGCGG UUGU UACUGAU UU UAUUCCGCAGACGACUCGCCCGA 4256. .H04
TTTTC ACTTTTTCTTTG CC GGGAGGACGAUGCGG UUUUCACUUUUUCUUUGCCCAGACGACUC6CCCG A 4257 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected se uenc mATTTGTATTTTATCCCC GGGAGGACGAUGCGGU UCAUUUGUAUU UUAUCCCCCAGACGACUCGCCCGA 4258_HQ4
CACCATTTTGT[TTTCTCC6 GGGAGGACGAUGCGGCACCAUUUUGUUUUUCUCCGCAGACGACUCGCCCGA 4259_H04
TTCCTTCATTATTCTTGCCG GGGAGGACGAUGCGGUUCCUUCAUUAUUCUUGCCGCAGACGACUCGCCCGA 4260..H04
TTCTATATTTTATTCTTCTG GGGAGGACGAUGCGGU UCUAUAUUUUAUUCUUCUGCAGACGACUCGCCCGA 426I_HG
TTTC AT A CTTGTTTTCTG C C GGGAGGACGAUGCGGUUUCAUACUUGUU UUCUGCCCAGACGACUCGCCCGA 4262_H04
TTTGTTCTTCTCTTTTTCCG GGGAGGACGAUGCGGUUUGUUCUUCUCUUUUUCCGCAGACGACUCGCCCGA. 4263_ H04
TCTCATCTTTTTTTTTTCCG GGGAGGACGAUGCGGUCUCAUCUUU UUUUU UUCCGCAGACGACUCGCCCGA 4264_H04
CCCTTTATTTGCTTATTCCG GGGAGGACGAUGCGGCCCUUUAUUUGCUUAU UCCGCAGACGACUCGCCCGA 426S_H04
CTCTCTTTTTATTCTTTCCG GGGAGGACGAUGCGGCUCUCUU UUUAUUCUU UCCGCAGACGACUCGCCCGA 267_H04
ACTTTTACTTTATCCTGCTG GGGAGGACGAUGCGGACUU UUACUUUAUCCUGCUGCAGACGACUCGCCCGA 4268„H04
TTTGGGAGTAGGGTGTTTCG GGGAGGACGAUGCGGUUUGGGAGUAGGGUGUUUCGCAGACGACUCGCCCGA 4269_H04
TTTTCCATTTTTTTCACTCCG GGGAGGACGAUGCGGU UUUCCAUUUUUUUCACUCCGCAGACGACUCGCCCGA 4270_H04
GGTGAAGGGT.6ATGGGTCCG GGGAGGACGAUGCGGGGUGAAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 4271__H04
TTTGTTTTTCCCTTTTTCCG GGGAGGACGAUGCGGU UUG UUUUUCCeUUU UUCCGCAGACGACUCGCCCGA 4272_H04
CTTATGGAGTAGGGAGGCGC GGGAGGACGAUGCGGCU UAUGGAGUAGGGAGGCGCCAGACGACUCGCGCGA 4274_H04
CCATCTCTTTTTTTTCCTCCG GGGAGGACGAUGCGGCCAUCUCUUUUUUUUCCUCCGCAGACGACUCGCCCGA 4275„HQ4
TTTG TTTTTTGTGCTTTTCCC GGGAGGACGAUGCGGUUUGUUUU UUGUGCUUUUCCCCAGACGACUCGCCCGA 4276_ H04
CTTTTTTAGATCTTTTTCCG GGGAGGACGAUGCGGCUUUUU UAGAUCU UUUUCCGCAGACGACUCGCCCGA 4277_H04
GGGAGGACGAUGCGGCAACU UUUCUUUUUAU UCCCCAGACGACUCGCCCGA 427S_H04
CTTTTTCTTTTCCCTTTCCG GGGAGGACGAUGCGGCUUU UUCUUUUCCCUUUCCGCAGACGACUCGCCCGA 4279_H04
TTTTCTATCTTTTTGTCCG GGGAGGACGAUGCGGUUUUCUAUCUUUUUG UCCGCAGACGACUCGCCCGA 4280_H04
CTTTTCTCTTCTTGTCTACCG GGGAGGACGAUGCGGCUUU UCUCUUCUUG UCUACCGCAGACGACUCGCCCGA 428l_H04
TTTGAAGTGTGATGTGTCCG GGGAGGACGAUGCGGU UUGAAGUGUGAUGUGUCCGCAGACGACUCGCCCGA 4282_H04
TCTTTTTATTTGCCTTTCCG GGGAGGACGAUGCGGUCUUUUUAU UUGCCUUUCCGCAGACGACUCGCCCGA 4283_H04
CCTTTTAACATG TTTTTCC6 GGGAGGACGAUGCGGCCUUU UAACAUG UUUUUCCGCAGACGACUCGCCCGA 4284_H04
TTTCATTTTTCTCTACTTCCG GGGAGGACGAUGCGGU UUCAUU UUUCUCUACUUCCGCAGACGACUCGCCCGA 4285 HQ4
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Figure imgf000293_0001
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Figure imgf000293_0002
# iri H4
HTS read variable region NA sequences # in H3 selection selection Selected sequenc TTTATTTCTCGnGTTGTG GGGAGGACGAUGCGGUUUAUUUCUCGU UGU UGUGCAGACGACUCGCCCGA 4313_H04
AGTTTTTGCCTTGTTGTCCG GGGAGGACGAUGCGGAGU UUUUGCCUUGUUGUCCGCAGACGACUCGCCCGA 4314_ HQ4
G TTTTC CACTTTTTTATCCC GGGAGGACGAUGCGGG UUUUCCACUUUU UUAUCCCCAGACGACUCGCCCGA 4315_H04
TCATCACTTTTCTTCTGCCG GGGAGGACGAUGCGGUCAUCACUUUUCUUCUGCCGCAGACGACUCGCCCGA 4316„H04
GTTTTGTCTTTTTTCCTTCCG GGGAGGACGAUGCGGG UUUUGUCUUUUUUCCU UCCGCAGACGACUCGCCCGA 4317_H04
TCACATTTAT TCTGCTG GGGAGGACGAUGCGGUCACAUUUAUUUU UCUGCUGCAGACGACUCGCCCGA 4318__H04
TTTGGGAGGAGGGGGGTCCG GGGAGGACGAUGCGGU UU6GGAGGAGGGGGGUCCGCAGACGACUCGCCCGA 4319_H04
TTCCTTATTTTTTCCCTCCG GGGAGGACGAUGCGGU UCCUUAUUUUUUCCCUCCGCAGACGACUCGCCCGA 4320_H04
CTTTTCTTCGCTTTA CCC GGGAGGACGAUGCGGCUUUUCUUCGCUUUACCGCAGACGACUCGCCCGA 4321_H04
CCTTTTAC CACTTTCCTCC6 GGGAGGACGAUGCGGCCUUUUACCACUUUCCUCCGCAGACGACUCGCCCGA 4322_H04
TTTCT A ATTTTTG TC GTG TG GGGAGGACGAUGCGGUUUCUAAUU UUUGUCGUG UGCAGACGACUCGCCCGA 4323_H04
CCTTTCGTTCTCCCTCCC GGGAGGACGAUGCGGCCUUUCGUUCUCCCUCCCCAGACGACUCGCCCGA 4324_ H04
TCCTTTCGTTCGGCCCTCCC GG6AGGACGAUGCGGUCOJUUCGUUCGGCCCUCCCCAGACGACUCGCCCGA 4325_B04
CCTTTC6TTCTG CTCTCCC GGGAGGACGAUGCGGCCUUUCG UUCUGCUCUCCCCAGACGACUCGCCCGA 4326_H04
TTTCTCTTTTCCTCTTTCCG GGGAGGACGAUGCGGUUUCUCU UU UCCUCUUUCCGCAGACGACUCGCCCGA 4327_H04
TTTCTTACTAGTTTTCrGCC GGGAGGACGAUGCGGUUUCUUACUAGUU UUCUGCCCAGACGACUCGCCCGA 4328_H04
TCTTTCTG TCTTTTG CTCCG GGGAGGACGAUGCGG'JCUU UCUGUCUUUUGCUCCGCAGACGACUCGCCCGA 4329 _HG4
AATTATTG7TFTT I T GTGACCG GGGAGGACGAUGCGGAAUUAUUGU UUUUUUGUGACCGCAGAGGACUCGCCCGA 4330_H04
ATCTrTTCCTTCTTCTGCCG GGGAGGACGAUGCGGAUCU UUUCCUUCUUCUGCCGCAGACGACUCGCCCGA 4331_H04
TTTG GG AGTAG G GTATTCCG GGGA6GACGAUGCGGUUUGGGAGUAGGGUAUUCCGCAGACGACUCGCCCGA 4334_ H04
TCCTGTCGTCTGTTCG TAAC GGGAGGACGAUGCGGUCCUG UCGUCUGUUCGUMCCAGACGACUCGCCCGA 4335„H04
TACTTTCCTTGTTTTCTCCG GGGAGGACGAUGCGGUACUUUCCUUGUUUUCUCCGCAGACGACUCGCCCGA 4336_HQ4
TTTGAAGGGGTGATGGGTCCT GGGAGGACGAUGCGGU UUGAAGGGGUGAUGGGUCCUCAGACGACUCGCCCGA 4337JH04
ATTTGGAGGAGGAGGTTCCC GGGAGGACGAUGCGGAUUUGGAGGAGGAGGUUCCCCAGACGACUCGCCCGA 4339_H04
ACTCTG 1 Π 'ΠΊ i TCCCTTCCG GGGAGGACGAUGCGGACUCUGU UUUUUU UCCCUUCCGCAGACGACUCGCCCGA 434Q_ H04
ACTTTTTCATTCTCTCTGCC GGGAGGACGAUGCGGACUUUUUCAUUCUCUCUGCCCAGACGACUCGCCCGA 4341 H04
# irt H4
HTS read variable region RNA sequences 13 selection selection Selected sequenc
CCTTTCATGCATTTTCTCCG GGGAGGACGAUGCGGCCUUUCAUGCAUUUUCUCCGCAGACGACUCGCCCGA 4342 H04
ACATTTCG I ! l l l I CTCGCCG GGGAGGACGAUGCGGACAUUUCGUUUUUUCUCGCCGCAGACGACUCGCCCGA 4343 ΗΌ4
TTTTATTTGCCCCTTTTCCC GGG AGG ACGAUGCGG U UU U AU U U GCCCCU U U UCCCCAG ACG AC UCG CCCG A 4344_H04
ACTTTTGATCTTTTACACCG GGGAGGACGAUGCGGACUU UUGAUCUUUUACACCGCAGACGACUCGCCCGA 4345__H04
ATCTTTTTCTTTTTCCACCA GGGAGGACGAUGCGGAUCU UUU UCUUUUUCCACCACAGACGACUCGCCCGA 4346_H04
ATTTCTCTTATTTCCTCCGC GGGAGGACGAUGCGGAUUUCUCUUAUUUCCUCCGCCAGACGACUCGCCCGA 4347_H04
ATTTCCTTTTACCTTCACCG GGGAGGACGAUGCGGAUUUCCUUUUACCUUCACCGCAGACGACUCGCCCGA 4348_H0
TTTCACCTCTTTTTCTGCCG GGGAGGACGAUGCGGUUUCACCUCUUUUUCUGCCGCAGACGACUCGCCCGA 4349_H04
'fTACTCATTTTATTTTCTCCG GGGAGGACGAUGCGGUUACUCAUUUUAUUUUCUCCGCAGACGACUCGCCCGA 4350_H04
ACGTTTACATTCTTTCACCG GGGAGGACGAUGCGGACGUUUACAUUCUU UCACCGCAGACGACUCGCCCGA 4351_H04
GCCTTTCGTTCTTTTCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUU UCUCCCCAGACGACUCGCCCGA 4352_H04
CATTCTTTGTTGTTTCTCGC GGGAGGACGAUGCGGCAUUCUUUGUUGU UUCUCGCCAGACGACUCGCCCGA 4353_H04
TTTTATGTTGATTTGTGTG GGGAGGACGAUGCGGUUUUAUGUUGAUUUGUGUGCAGACGACUCGCCCGA 4354_H04
TTTTATGGGTGATG6GTCCG GGGAGGACGAUGCGGU UUUAUGGGUGAUGGGUCCGCA6ACGACUCGCCCGA 4356JH04
TCTTCTTGTTACTCGTTCCG GGGAGGACGAUGCGGUCUUCUUGUUACUCGUUCCGCAGACGACUCGCCCGA 4357._H04
GCCTTTCGTTCTACCTTCCC GGGAGGACGAUGCGGGCCUUUCG UUCUACCIJUCCCCAGACGACUCGCCCGA 4358_H04
TATTTACCTTTCTTTCCCCG GGGAGGACGAUGCGGUAUUUACCUUUCUUUCCCCGCAGACGACUCGCCCGA 4359_H04
TTACTT ATTTCTTCTTTG cc GGGAGGACGAUGCGGUUACUUAUUUCUUCUU UGCCCAGACGACUCGCCCGA 4360_H04
TTTTTCGTGTTTTTTTG CACC GGGAGGACGAUGCGGUUUU UCGUG UUUUUUUGCACCUCAGACGACUCGCCCGA 4361_H04
GCTGAAGGGTGAl'GGGTCCG GGGAGGACGAUGCGGGCU6AAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 4362_H04
CmCACTTTTTTTCTCCG GGGAGGACGAUGCGGCUUUCACUUUUU UUCUCCGCAGACGACUCGCCCGA 4363_H04
GTTGATGGGTGATGGGTCCG GGGAGGACGAUGCGGGUUGAUGGGUGAUGGGUCCGCAGACGACIJCGCCCGA 4364JHQ4
TATTTTCTTCTTTTCTCCG GGGAGGACGAU6CGGUAUUU UCUUCUUUUCUCCGCAGACGACUCGCCCGA 436δ_Η04
CCTTTTACTTTTTCTCTCCG GGGAGGACGAUGCGGCCUUUUACUUUUUCUCUCCGCAGACGACUCGCCCGA 4367_H04
TCTTGTTTTTTTTTTGCCGC GGGAGGACGAUGCGGUCUUG UUUUU UUUUUGCCGCCAGACGACUCGCCCGA 436S_ H04
GCCGTTCGGTCrGCCCTCCC GGGAGGACGAUGCGGGCCGU UCGGUCUGCCCUCCCCAGACGACUCGCCCGA 4371 . H04
# in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc TC ACTCTTTCTCTTTTG CC GGGAGGACGAUGCGGAUCACUCUUUCUCUUUUGCCCAGACGACUCGCCCGA 4372_H04
TTGGGTGTAGGGTGGTAGC GGGAGGACGAUGCGGUUGGGUGUAGGGUGGUAGCCAGACGACUC6CCCGA 4374 H04
TTTTTGTTTTGTTTTGGTG GGGAGGACGAUGCGGU UUU'UGUUUUGUUUUGGUGCAGACGACUCGCCCGA 4375_H04
G CTAC'iTTTTACTCTTTCCG GGGAGGACGAUGCGGGCUACUUUUUACUCUUUCCGCAGACGACUCGCCCGA 4377„H04
ACATTCTCTTTTTTTTTCACCG GGGAGGACGAUGCGGACAU UCUCUUUUUUUU UCACCGCAGACGACUCGCCCGA 4378_H04
CCTTTTCTGTCTTCTACCCG GGGAGGACGAUGCGGCCUU UUCUGUCUUCUACCCGCAGACGACUCGCCCGA 4379_H04
ACTTTA ACATTCTTCTTC CG GGGAGGACGAUGCGGACUUUAACAUUCUUCUUCCGCAGACGACUCGCCCGA 4380_H04
TCGTTGTCTTTT1TTTTGCCC GGGAGGACGAUGCGGUCGUUGUCUUUUUUUUUGCCCCAGACGACUCGCCCGA 4381_ H04
ATTTCCTAATG CTTCTACCG GGGAGGACGAUGCGGAUUUCCUAAUGCUUCUACCGCAGACGACUCGCCCGA 43S2_H04
GTTCCATTTTTTTTTTCCACC6 GGGAGGACGAUGCGGGUUCCAUUUUUUUUUUCCACCGCAGACGACUCGCCCGA 4383_H04
TTTTTCTCACAATTCTCATCCT GGGAGGACGAUGCGGUUUUUCUCACAAUUCUCAUCCUCAGACGACUCGCCCGA 43g4_H04
TTTTTATTTTCCTG G TTCCG GGGAGGACGAUGCGGUUU UUAUU UUCCUGG UUCCGCAGACGACUCGCCC6A 4385_H04
GTTTACTTTTTCTCCATTCC. G GGAGG ACG AUG CGGG UUUACUUU UUCUCCAU UCCC AG ACG AC UCG CCCG A 4386_H04
TGTTTTCTCTTTGTTTACTG GGGAGGAC6AUGCGGUGUUUUCUCUUUGUUUACUGCAGACGACUCGCCCGA 4387_HG4
TTTCTTTATATTTATGCCCC GGGAGGACGAUGCGGU UUCUUUAUAUUUAUGCCCCCAGACGACUCGCCCGA 388_H04
TCTHTACAAACACTCTCCG GGGAGGACGAUGCGGUCUUUUACAAACACUCUCCGCAGACGACUCGCCCGA 4389_H04
CCTTAATTTTCACTTTCCCG GGGAGGACGAUGCGGCCUUAAUUUUCACUUUCCCGCAGACGACUCGCCCGA 439O„H04
TTTTCTGTTTTGTTCATCCG GGGAGGACGAUGCGGU UUUCUGUUUUGUUCAUCCGCAGACGACUCGCCCGA 4 9l_H04
AGTTTTTATTTCTATTTTGCC GGGACGACGAUGCGGAGUU UUUAU UUCUAUUUUGCCCAGACGACUCGCCCGA 4392_H04
TGTTTTAGTTCTTTTTCTCG C GGGAGGACGAUGCGGUG UUUUAGUUCUUUU UCUCGCCAGACGACUCGCCCGA 4393JH04
CCACTTAAAT rCTCCG GGGAGGACGAUGCGGCCACUUAAAUUUUUUCUCCGCAGAC6ACUCGCCCGA 4394_H04
ATCTTACTACTTTTCTCCG C GGGAGGACGAUGCG6AUCUUACUACUUUUCUCCGCCAGACGACUCGCCCGA 4395__H04
TCTTTCACTATTTTTATCCC GGGAGGACGAUGCGGUCUUUCACUAUUUU UAUCCCCAGACGACUCGCCCGA 4396_H04
CCTTACTTACτTTACπ"CCG GGGAGGACGAUGCGGCCUUACUUACUUUACUUCCGCAGACGACUCGCCCGA 4397_H04
ACACTTTTTTTTTCACTTCCC GGGAGGACGAUGCGGACACUU UUUU UUUCACUUCCCCACACGACUCGCCCGA 439S_H04
TTTCTTCTATCTTTTTCCCC GGGAGGACGAUGCGGUUUCUUCUAUCUUUUUCCCCCAGACGACUCGCCCGA 4399 H04
# in H4
NTS read variable region RNA sequences selection Selected sequenc CCATTTTAATTCTTTTACCe GGGAGGACGAUGCGGCCAUUUUAAUUCUUUUACCGCAGACGACUCGCCCGA 4400„H04
TCTTTTTTTTT ACTTTCC CCG GGGAGGACGAUGCGGUCUUUU UU UUUACUUUCCCCGCAGACGACUCGCCCGA 4401_H04
TTATTTACTTCCTTTTCACAC GGGAGGACGAUGCGGUUAUUUACUUCCUUUUCACACCAGACGACUCGCCCGA 4402_H04
ATTTTGACCTTTTTTACCCG GGGAGGACGAUGCGGAUUUUGACCUUUUUUACCCGCAGACGACUCGCCCGA 4403_H04
TTTTTTCTTGACTTTATCTG GGGAGGACGAUGCGGUUU UUUCUUGACUUUAUCUGCAGACGACUCGCCCGA 4404__H04
TTTTGTTCTCTATTCCTCTG GGGAGGACGAUGCGGUUU UGUUCUCUAUUCCUCUGCAGACGACUCGCCCGA 4405_H04
CTTTTTTTTCTTTTG TT CCCG GGGAGGACGAU6CGGCUUU UUUUUCUUU UGUUCCCGCAGACGACUCGCCCGA 4406_HQ4
CCACTTACACACTTFTFCCG GGGAGGACGAUGCGGCCACU UACACACUUUUUCCGCAGACGACUCGCCCGA 44O7_H04
rrTTTCTATCTTTTTTG TCCG GGGAGGACGAUGCGGU UUUUCUAUCUUUUU UGUCCGCAGACGACUCGCCCGA 4408_H04
ATC I 1 1 1 i CTCTTACCGCC GGGAGGACGAUGCGGAUCUUU UUUUCUCUUACCGCCCAGACGACUCGCCCGA 4409_H04
GGTTTTTCGCTTTATTTCCC GGGAGGACGAUGCGGGGUUUUUCGCUUUAUUUCCCCAGACGACUCGCCCGA 4410_H04
TATCG CTTACTTT ATTTC C G GGGAGGACGAUGCGGUAUCGCUUACUUUAUU UCCGCAGACGACUCGCCCGA 44il„H04
GTTTATTTTTTTGTGTTCCG GGGAGGACGAUGCGGGUUUAUUU UUUUG UGUUCCGCAGACGACUCGCCCGA 4412_B04
TTATATTTCCTCTTCTTCCG GGGAGGACGAUGCGGUUAUAUUUCCUCUUCUUCCGCAGACGACUCGCCCGA 4413raH04
TCTTCTACTCATTTTTTCCG GGGAGGACGAUGCGGUCUUCUACUCAUUUUUUCCGCAGACGACUCGCCCGA 4414_.H04
ATT'iTACTTCACTTTGACCG GGGAGGACGAUGCGGAU UUUACUUCACUUUGACCGCAGACGACUCGCCCGA 4415_HQ4
ATTTTACTTTCTTCATCCCG GGGAGGACGAUGCGGAUUUUACUUUCUUCAUCCCGCAGACGACUCGCCCGA 4416_H04
TCTAACTATTCTTTCCTCCG GGGAGGACGAUGCGG'JCUAACUAUUCUUUCCUCCGCAGACGACUCGCCCGA 4417_H04
GACCTTTCTTG TTTCTACC C GGGAGGACGAUGCGGGACCU UUCUUGUUUCUACCCCAGACGACUCGCCCGA 4418_H04
CTTTTCATTTCAATTCTCCG GGGAGGACGAUGCGGCUUU UCAUUUCAAUUCUCCGCAGACGACUCGCCCGA 4419_H04
CTTTGTCTATTTTTTTTTTCCG GGGAGGACGAUGCGGCUUUGUCUAUUUUUU UUUJCCGCAGACGACUCGCCCGA 4420_H04
TCTTTACTATATTTCACCCG GGGAGGACGAUGCGGUCUUUACUAUAUUUCACCCGCAGACGACUCGCCCGA 4421_H04
CTTTTCTTTCTCTTTTCCG GGGA6GACGAUGCGGCU UUUCUUUCUCUUUUCCGCAGACGACUCGCCCGA 4422_H04
ACTTTTATCCATTTATCCCG GGGAGGACGAUGCGGACUUUUAUCCAUUUAUCCCGCAGACGACUCGCCCGA 4425_H04
GAACTC I I ί I I I ! I I CGCCC G6GAGGACGAUGCGGGAACUCUUUUUUUUUC6CCCCAGACGACUCGCCCGA 4426...H04
ACTTGTTC CTTCTTTCGCCG GGGAGGACGAUGCGGACUUG UUCCUUCUUUCGCCGCAGACGACUCGCCCGA 4427 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CTCTTACACTTCCCTTACCG GGGAGGACGAUGCGGCUCUUACACUUCCCUUACCGCAGACGACUCGCCCGA 4428. _H04
GTTTTTCG CTGCCCTCCC GGGAGGACGAUGCGGGUUUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 4430. _H04
TGCCGTAGTCTATCCGCCCG GGGAGGACGAUGCGGUGCCG UAGUCUAUCCGCCCGCAGACGACUCGCCCGA 4431. .H04
TTTTATTTCTTTTTTTCCCCC GGGAGGACGAUGCGGUUUUAUUUCUUUUUUUCCCCCCAGACGACUCGCCCGA 4432. _HQ4
CC ATTTTGTTTTTG TTTCCG GGGAGGACGAUGCGGCCAUUUUGUUUUUGU UUCCGCAGACGACUCGCCCGA 4433_ _H04
TTTCTCTTTCTTTTCTCCCC GGGAGGACGAUGCGGUUUCUCU UUCUUU UCUCCCCCAGACGACUCGCCCGA 4434_ _H04
ACCTTATTTTTTGTTTGCCG GGGAGGACGAUGCGGACCUUAUUUUUUGUUUGCCGCAGACGACUCGCCCGA 4435. .H04
ATTTTTACTG TTCTCTTTCC C GGGAGGACGAUGCGGAUU'JUUACUGUUCUCUUUCCCCAGACGACUCGCCCGA 4436. .H04
CTTTTATTATCTCTTTCCGC GGGAGGACGAUGCGGCU UUUAU UAUCUCUUUCCGCCAGACGACUCGCCCGA 4437 , .H04
TTCTCTTCTTTCTTTCCCCG GGGAGGACGAUGCGGU UCUCUUCUUUCUUUCCCCGCAGACGACUCGCCCGA 4438. _H04
ATTTCAATCTTTTCCTACCG GGGAGGACGAUGCGGAUUUCAAUCUUUUCCUACCGCAGACGACUCGCCCGA 4439. .H04
TTTTCTATTTCTCTTCTGTA GGGAGGACGAUGCGGUUUUCUAUUUCUCU UCUGUACAGACGACUCGCCCGA 4440. .H0
CATTTCTATTATTCCCTCG C GGGAGGACGAUGCGGCAUUUCUAUUAU UCCCUCGCCAGACGACUCGCCCGA 4441. .H04
TTATTATTCTTTTCTTCG CC GGGAGGACGAUGCGGUUAUUAUUCUUUUCUUCGCCCAGACGACUCGCCCGA 4442. „H04
TTTCTCTTTTTTCTCTTTCCG GGGAGGACGAUGCGGUU UCUCU UUUUUCUCU UUCCGCAGACGACUCGCCCGA 4443. .H04
CTTTTrCACGTTTTTTCCTG GGGAGGACGAUGCGGCUUU UUCACGUU UUUUCCUGCAGACGACUCGCCCGA 4444. _HG4
TTTTTGTTTGTTTTTGGTG GGGAGGACGAUGCGGUUUUUG UUUGUUUUUGGUGCAGACGACUCGCCCGA 4445. H04
AATTATCCTTTTTCACCCCG GGGAGGACGAUGCGG UUAUCCUU UUUCACCCCGCAGACGACUCGCCCGA 4446. .H04
TTTCTTTTTATTTCCCCTG GGGAGGACGAUGCGGUUUCUUU UUAUUUCCCCUGCAGACGACUC6CCCGA 4447. .H04
CCTATACCTGTTTCmCCG GGGAGGACGAUGCGGCCUAUACCUGUUUCUUUCCGCAGACGACUCGCCCGA 4448. _H04
TTTTTCTTTTCC CTTG TC CG GGGAGGACGAUGCGGUUUU UCU UUUCCCUUGUCCGCAGACGACUCGCCCGA 4449. _H04
TTTCTCTTTTTCTCTTCCG GGGAGGACGAUGCGGUUUCUCU UUU UCUCUUCCGCAGACGACUCGCCCGA 4450. _H04
TTTTTTTTCCTGCTiGTCCG GGGAGGACGAUGCGGUUUU UUUUCCUGCUUGUCCGCAGACGACUCGCCCGA 4451. „H04
TCCTTTCGTTTTGCCCTCCC GGGAGGACGAUGCGGUCCUU UCGUUUUGCCCUCCCCAGACGACUCGCCCGA 4452. .H04
ΤΠ GTAGGGTCATGGGTCCG GGGAGGACGAUGCGGUUUGUAGGGUCAUGGG UCCGCAGACGACUC6CCCGA 4453. _H04
Tl'TCAAGGGCGATGGGTCCG GGGAGGACGAUGCGGUU UCAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 4454. _H04
# in H4
HTS read variable region RNA sequences selection Selected sequenc TCATCTTTTTACTTCCTGTG GGGAGGACGAUGCGGUCAUCliUUUUACUUCCUGUGCAGACGACUCGCCCGA 4455_H04
ATTTTCTTTTTGCTTG CCG GGGAGGACGAUGCGGAUUUUCUUUUUGCUUGCCGCAGACGACUCGCCCGA 4456_H04
TTTTTT I'GTCTTGCTCCG GGGAGGACGAUGCGGUUUUUUUUGUCUUGCUCCGCAGACGACUCGCCCGA 4457_H04
CTCCATTCTATCTTTTTCCG GGGAGGACGAUGCGGCUCCAUUCUAUCUUUUUCCGCAGACGACUCGCCCGA 4 59_H04
ATTCCTTTTCCTTTTTACCG GGGAGGACGAUGCGGAU UCCUUUUCCUUUUUACCGCAGACGACUCGCCCGA 4461 H04
ATTTCTCTTTTTTCTG CCG GGGAGGACGAUGCGGAU UUCUCUU UUUUCUGCCGCAGACGACUCGCCCGA 4462_H04
GAACTC I I I Π ! I i ITTCG CCC GGGAGGACGAUGGGGGAACUCUU UU UUUUUUUCGCCCCAGACGACUCGCCCGA 4464_H04
TTTGAAGGGt rCGGTCCG GGGAGGACGAUGCGGU UUGAAGGGUUUUGGGUCCGCAGACGACUCGCCCGA 4465„H04
TTTCTCTACGTT TCTGTCCG GGGAGGACGAUGCGGU UUCUCUACGUUUUCUG UCCGCAGACGACUCGCCCGA 4466_H04
TTATTCTTGTTTTACCCCTG GGGAGGACGAUGCGGUUAU UCUUGU UUUACCCCUGCAGACGACUCGCCCGA 4467_H04
TTTTTGTTCTTTTrrCTTCC GGGAGGACGAUGCGGUUUU UGUUCUUUUUUCU UCCGCAGACGACUCGCCCGA 446.8_H04
CCTTTTG TATTGTTATTCC G GGGAGGACGAUGCGGCCUUUUGUAUUGUUAU UCCGCAGACGACUCGCCCGA 4469_H04
TCTGCTC I 'i I f 1 1 i l !CCCCC GGGA6GACGAUGCGGUCUGCUCU UUUUUUUUCCCCCCAGACGACUCGCCCGA 4470_H04
AGTTTTTTTAATTTCTGTCCC GGGAGGACGAUGCGGAG UUU UUUUAAUUUCU6UCCCCAGACGACUCGCCCGA 4471JH04
TCACTAATTCTTTTCTTCCG GGGAGGACGAUGCGG UCACUAAUUCUUUUCUUCXGCAGACGACUCGCCCGA 4472_H04
TTTTCTACACTTCTTTTTCCG GGGAGGACGAUGCGGUU UUCUACACUUCUU UUUCCGCAGACGACUCGCCCGA 4473_H04
TTTTATCCTTCTACTGCCCG GGGAGGACGAUGCGGUUUUAUCCUUCUACUGCCCGCAGACGACUCGCCCGA 4474_H04
TTTCTTTTTTCTTACTTG CC GGGAGGACGAUGCGGUUUCUUU UUUCUUACUUGCCCAGACGACUCGCCCGA 4475_H04
TTCTAGCTTCTTTTTTTG TCCG GGGAGGACGAUGCGGUUCUAGCU UCUUUU UU UGUCCGCAGACGACUCGCCCGA 4476_H04
TTTTATTTTCTTTTCGCCG GGGAGGACGAUGCGGUUUUAUUUUCUUUUCCCCGCAGACGACUCGCCCGA 4477 JH04
ACATTCTTTATTTACCACCG GGGAGGACGAUGCGGACAUUCUUUAUUUACCACCGCAGACGACUCGCCCGA 4478„H04
TCTTTTTGTTTGTTTTGCTC GGGAGGACGAUGCGGUCUUU UUG UUUGUUUUGCUCCAGACGACUCGCCCGA 4480_H04
TTTGTGAGTA6GGTGTTCCG GGGAGGACGAUGCGGUU UGUGAGUAGGG UGUUCCGCAGACGACUCGCCCGA 4481_H04
TCACATCTAATTTTTTCTCCG GGGAGGACGAUGCGGUCACAUCUAAUUU UUUCUCCGCAGACGACUCGCCCGA 4484_H04
TTACTTG GTTATTTTTTTCTG GGGAGGACGAUGCGGUUACUUGGUUAUUUUUUUGUGCAGACGACUCGCCCGA 4485_H04
TTTGAAGGGTGATAGGGTCCT GGGAGGACGAUGCGG UU UGAAGGGUGAUAGGGLCCUCAGACGACUCGCCCGA 4486 HQ4
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# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TCCCCTGAGTTTCTGCTGCC GGGAGGACGAUGCGGUCCCCUGAGUUUCUGCUGCCCAGACGACUCGCCCGA 4521 _H04
TG GTTTTCTCTTCTTTTGCC GGGAGGACGAUGCGGUGGUU UUCUCUUCUU UUGCCCAGACGACUCGCCCGA 4522_H04
TACTTCTTTTTTT CCTCTCCG GGGAGGACGAUGCGGUACUUCUUU UUUUCCUCUCCGCAGACGACUCGCCCGA 4524„H04
TTTCCT TTCTCTTGTCGG GGCAGGACGAUGCGGUUUCCUUUU UCUCUUG UCGGCAGACGACUCGCCCGA 4525_H04
GTTTACTTCTTTCTCTGCTG GGGAGGACGAUGCGGGUU UACUUCUUUCUCUGCUGCAGACGACUCGCCCGA 4526_H04
AATTATGTTTTiT I ' '!' GTGACCT GGGAGGACGAUGCGGAAUUAUG UUUUUU UUGUGACCUCA6ACGACUCGCCCGA 4527„H04
TTTTATCCTTTTCCG CTCCG GGGAGGACGAUGCGGU UUUAUCCUUUUCCGCUCCGCAGACGACUCGCCCGA 4528_HG4
CTTTTTGATTCTTGTTTCCG GGGAGGACGAUGCGGCUUU UUGAU UCUUGUUUCCGCAGACGACUCGCCCGA 4529JH04
CTCTTTCTTCTTTTCTCCG GGGAGGACGAUGCGGCUCUUUCUUCUU UUCUCCGCAGACGACUCGCCCGA 4530_H04
GCCTTTCGTTCTTGCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUUGCCUCCCCAGACGACUCGCCCGA 4531_H04
GCCCTGTCGTCTTACGTCeC GGGAGGACGAUGCGGGCCCUGUCGUCUUACGUCCCCAGACGACUCGCCCGA 4533JHG4
TCTTTACTTTCTCTTG C CG GGGAGGACGAUGCGGUCUUUACUUUCUCUUGCCGCAGACGACUCGCCCGA 4534 JHG4
o ACTTTCATATTTTTCCACCG GGGAGGACGAUGCGGACUUUCAUAUUU UUCCACCGCAGACGACUCGCCCGA 4535.. H04
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CTTTTCGTTCTG CCCTCCC GGGAGGACGAUGCGGCUUU UCGUUCUGCCCUCCCCAGACGACUCGCCCGA 4536„H04
TGTTTATTTTCTCCGTGCCC GGGAGGACGAUGCGG UGUUUAUUUUCUCCGUGCCCCAGACGACUCGCCCGA 4538_HQ4
TCTTTTTATTCTCTCCCCCC GGGAGGACGAUGCGG UCUUUUUAUUCUCUCCCCCCCAGACGACUCGCCCGA 4540_H04
CCTTTGATCCTTTTTCTCCG GGGAG6ACGAUGCGGCCUUUGAUCCU UUUUCUCCGCAGACGACUCGCCCGA 4541_H04
TTTGG GAGTAGG GTCTCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGUCUCCGCAGACGACUCGCCCGA 4543_H04
TCTC TTACTTCCTTTTCCG GGGAGGACGAUGCGGUCUCUUUACUUCCUUUUCCGCAGACGACUCGCCCGA 4544_H04
TGACTCGTCTTTTTCGTCCC GGGAGGACGAUGCGGUGACUCGUCUUUUUCGUCCCCAGACGACUCGCCCGA 454S_H04
ATTCTCTTCTCTTTTCACCG GGGAGGACGAUGCGGAUUCUCUUCUCUUUUCACCGCAGACGACUCGCCCGA 4546_H04
CGCTTTTCTTACTTCTTCCG GGGAGGACGAUGCGGCGCUU UUCUUACUUCUUCCGCAGACGACUCGCCCGA 4547_H04
CTTTCTCTTG CT CTTTC CG GGGAGGACGAUGCGGCUUUCUCUUGCUCUUUCCGCAGACGACUCGCCCGA 4548_H04
TTTACTTTTA CTCTTG CCC GGGAGGACGAUGCGGUU UACUUUUACUCUUGCCCCAGACGACUCGCCCGA 4549_H04
CTTTATTATCCTTTTTTCCC GGGAGGACGAUGCGGCUUUAUUAUCCUUUUUUCCCCAGACGACUCGCCCGA 4S5Q_ HG4
CTCTTTATTTCCTTGCCCCG GGGAGGACGAUGCGGCUCUUUAUUUCCUUGCCCCGCAGACGACUCGCCCGA 4551 H04
# in H4
HT5 read variable region RNA sequences 3 selection selection Selected sequenc TTTCTTTG CTTTCTCTCCG GGGAGGACGAUGCGGUUUCUU UGCUUUCUCUCCGCAGACGACUCGCCCGA 4552 H04
ATTTCCCCTTTCTTTTGCCG GGGAGGACGAUGCGGAUUUCCCCUUUCUUUUGCCGCAGACGACUCGCCCGA 4553_H04
TTTG G GGTTTTC G TG CC G GGGAGGACGAUGCGGU UUGGG6U UUUCGUGCCGCAGACGACUCGCCCGA 4554_H04
ATCCTTTTGCTTTCTACCG GGGAGGACGAUGCGGAUCCU UUUGCU UUCUACCGCAGACGACUCGCCCGA 4555„H04
TCCTTTATTGTTTTCTCCTG GGGAGGACGAUGCGGUCCUUUAUUGUUUUCUCCUGCAGACGACUCGCCC6A 4556_H04
TATTCTTCTATACCTCTCCG GGGAGGACGAUGCGGUAUUCUUCUAUACCUCUCC6CAGACGACUCGCCCGA 4557„H04
GTTTCCTTGTTTCTTGTTCCG G6GAGGACGAUGCGGGUUUCCU UGUUUCUUGUUCCGCAGACGACUCGCCCGA 4558_H04
CTTTTCCAGTCTTTTTCCCG GGGAGGACGAUGCGGCUUUUCCAGUCUUUUUCCCGCAGACGACUCGCCCGA 4559_H04
TTTCTCTTG CTT A CTC CCC G GGGAGGACGAUGCGGUUUCUCU UGCUUACUCCCCGCAGACGACUCGCCCGA 4560_H04
ATTG AAGG GTG ATG GGTCCC GGGAGGACGAUGCGGAUUGAAGGGUGAUGGGUCCCCAGACGACUCGCCCGA 4561_H04
TTTGGGGTCTTCCCGTGCCG GGGAGGACGAUGCGGUUUGGGGUCUUCCCGUGCCGCAGACGACUCGCCCGA 4564_H04
GCCTTTCGCTCTACCCTCCC GGGAGGACGAUGCGGGCCUU UCGCUCUACCCUCCCCAGACGACUCGCCCGA 4565_H04
GTCTTTTG GCTTATCGTCCC GGGAGGAC6AUGCGGG UCUUUUGGCUUAUCGUCCCCA6ACGACUCGCCCGA 4566_K04
ATTCTTTTCTCTTTCAGCG GGGAGGACGAUGCGGAUUCUUUUCUCUUUCACCGCAGACGACUCGCCCGA 4567_H04
CTTTTCCTTTTTTTTCACTCCG GGGAGGACGAUGCGGCUUUUCCUUUUUUUUCACUCCGCAGACGACUCGCCCGA 4568„H04
TCTTTACTTTTTCTTTGCCG GGGAGGACGAUGCGGUCUUUACUUUUUCU UUGCCGCAGACGACUCGCCCGA 4569_H04
TTTGAAGGGACATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGACAUGGG UCCGCAGACGACUCGCCCGA 4570_H04
C'CTTCACTTTTTCTCTCCG GGGAGGACGAUGCGGCUUUCACUUUUUCUCUCCGCAGACGACUCGCCCGA 4571_H04
TTTGCAGGGTGTTGGGTCCG GGGAGGACGAUGCGGUU UGCAGGGUGUUGGGUCC6CAGACGACUCGCCCGA 4572_HQ4
ATCCTTTCTTTCTTCTG CCG GGGAGGACGAUGCGGAUCCUUUCUUUCUUCUGCCGCAGACGACUCGCCCGA 4573_H04
TCTTTTCATGTCTCTCTCCG GGGAGGACGAUGCGG UCUUUUCAUG UCUCUCUCCGCAGACGACUCGCCCGA 4574_H04
TTTGAAGGGTATTG6GTCCG GGGAGGACGAUGCGGUUUGAAGGGUAUUGGGUCCGCAGACGACUC6CCCGA 4576_H04
TGTTATTTCCCTCTTCTCCG GGGAGGACGAUGCGGUGUUAUUUCCCUCUUCUCCGCAGACGACUCGCCCGA 4577_H04
GCCTTTCGTTCTTTCCTCCC GGGAGGACGAUGCGGGCCUU UCG U UCUUUCCUCCCCAGACGACUCGCCCGA 4578JH04
TTCTTTTCTCAG CTCTTCCG GGGAGGACGAUGCGGUUCUU UUCUCAGCUCUUCCGCAGACGACUCGCCCGA 4579„H04
CTCTTTTTCCCTTTCTCCG GGGAGGACGAUGCGGCUCUU UUUCCCUUUCUCCGCAGACGACUCGCCCGA 4580 H04
8 in H4
HTS read variable region R A sequences # in H3 selection selection Selected sequenc TG TTTTGTTCTCCCTGGCCG GGGAGGACGAUGCGGUGUUUUGUUCUCCCUGGCCGCAGACGACUCGCCCGA 4581. .H04
CTTGGAGGGTGATGGGTCCG GGGAGGACGAUGCGGCUUGGAGGGUGAUGGGUCCGCAGACGACUCGCCCGA 4582. .H04
TCTTTTCTTCTCTTA CCG GGGAGGACGAUGCGGUCUUUUCUUCUCUUACCGCAGACGACUCGCCCGA 4583. .H04
AGTTGTTTAATTTGTTTGCC GGGAGGACGAUGCGGAGUUGUUUAAUUUGUUUGCCCAGACGACUCGCCCGA 4584, .H04
TTrCCTTTCACCTTTCCCCC GGGAGGACGAUGCGGUUUCCUUUCACCUUUCCCCCCAGACGACUCGCCCGA 4585. .H04
ATTCTGTTTTTCTTGTTACCG GGGAGGACGAUGC6GAUUCUGUUUU UCU UGUUACCGCAGACGACUCGCCCGA 4586. _H04
ATTTCTTCTCCTCTTCTCCG GGGAGGACGAUGCGGAUUUCUUCUCCUCUUCUCCGCAGACGACUCGCCCGA 4587. _H04
TTTTATTACTTTTCCCTG G C GGGAGGACGAUGCGGUUUUAUUACUUUUCCCUGGCCAGACGACUCGCCCGA 4588. .H04
TTGAAGGGTGATGGTCCG GGGAGGACGAUGCGGUUG GGGUGAU6GUCCGCAGACGACUCGCCCGA 4589. _H04
TTCATTTCTTTTCTCTCCG GGGAGGACGAUGCGGUUCAU UUCUUU UCUCUCCGCAGACGACUCGCCCGA 4590, _H04
TTTG GGAGTAGGGTG TTTCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGUG UU UCCGCAGACGACUCGCCCGA 4591, „H04
CAT'iTTCTG TTTTTTC GGCC GGGAGGACGAUGCGGCAUUUUCUGUUUUUUCGGCCCAGACGACUCGCCCGA 4592. _HQ4
o TTTATCAA6TTTTT TCCG GGGAGGACGAUGCGGUUUAUCAAGUUUUUUUCCGCAGACGACUCGCCCGA 4593. _HQ4
TTTGTCTTTGTTTITGTCCG GGGAGGACGAUGC6GUUUGUCU UUGUUUUUGUCCGCAGACGACUCGCCCGA 4594. _H04
TTATTGCTTTTTTTTTGGTCCG GGGAGGACGAUGCGGUUAU UGCUUUUUUUUUGGUCCGCAGACGACUCGCCCGA 4595. ,H04
TTTATTTTCTGTTGTTGTG GGGAGGACGAUGCGGUUUAUUUUCUGUUGU UGUGCAGACGACUCGCCCGA 4596. .H04
GCTGGTGTA6GGG6GTTCCG GGGAGGACGAUGCGGGCUGGUG UAGGGGGGUUCCGCAGACGACUCGCCCGA 4598. .H04
CCATTCTTTCTCTGCTTCCG GGGAGGACGAUGCGGCCAUUCUU UCUCUGCUUCCGCAGACGACUCGCCCGA 4599. .H04
CTTGTTTCTTCCTATTACCG GGGAGGACGAUGCGGCUUGUUUCUUCCUAUUACCGCAGACGACUCGCCCGA 4600. .H04
τΑ ι'ττΑεαπετΑτταοεο GGGAGGACGAUGCGGUAUUUACGUUCUAUUCUCCG-CAGACGACUCGC.CCGA 4601. _HQ4
TCTTTATTACATTCTTTGCG GGGAGGACGAUGCGGUCUUUAU UACAUUCUUUGCGCAGACGACUCGCCCGA 4602. .H04
TTCTTGT'nTTACTCATCCC GGGAGGACGAUGCGGUUCUUGU UUUUACUCAUCCCCAGACGACUCGCCCGA 4603. .HQ4
CTTAC ATTTCCCTTTTTC CG GGGAGGACGAUGCGGCUUACAUUUCCCUUUUUCCGCAGACGACUCGCCCGA 4604. _H04
TACTATATCTTTTTTCGCTG GGGAGGACGAUGCGGUACUAUAUCUUUUUUCGCUGCAGACGACUCGCCCGA 4605. .H04
ATTGCGTACTT ΓΠ I I ! 1 1 CCG GGGAGGACGAUGCGGAUUGCGUACUUUUUUUUUUCGGCAGACGACUCGCCCG'A 4606. _H04
ATTCTTTAACTCTCTTCCCG GGGAGGACGAUGCGGAUUCU UUAACUCUCUUCCCGCAGACGACUCGCCCGA 4607 H04
# in H4
HTS read variable region RNA sequences H3 selection selection Selected sequenc AATTTG CTTCTTTTTTATCCG GGGAGGACGAUGCGGAAUUUGCUUCUUUUUUAUCCGCAGACGACUCGCCCGA 4608_H04
TGTTCTATTCGCTCfTTCCG GGGA6GACGAUGCGGUGUUCUAUUCGCUCUUUCCGCAGACGACUCGCCCGA 4609 H04
ATTACTTACCTTCTTTTGCC GGGAGGACGAUGCGGAUUACUUACCUUCUUUUGCCCAGACGACUCGCCCGA 461Q_HG4
CTTC6TTA AATTTTC CTCCG GGGAGGACGAUGCGGCU UCGUUAAAUUUUCCUCCGCAGACGACUCGCCCGA 4611_H04
CTCTTTGTTTTTTCTTG CTG GGGAGGACGAUGCGGCUCUUUG UUUUUUCUUGCUGCAGACGACUCGCCCGA 4612J-I04
GAAG GAGGAGTTG 6 G ATCTG GGGAGGACGAUGCGGGAAGGAG6AGUUGGGAUCUGCA6ACGACUCGCCCGA 4613_H04
TCTTTnXATTTGTTrCCC GGGAGGACGAUGCGGUCUUUUUCAUUUGUUUCCCCAGACGACUCGCCCGA 4614_HQ4
rnACTATTTTATTGCCCCG GGGAGGACGAUGCGGUUUACUAUUUUAUUGCCCCGCAGACGACUCGCCCGA 4615_H04
TACCTTTGTAATTTTTTCCG GGGAGGACGAUGCGGUACCU UUGUAAUUUUU UCCGCAGACGACUCGCCCGA 4616_H04
GCTTTTTACATTTCTCACCC GGGAGGACGAUGCGGGCULJUUUACAUUUCUCACCCCAGACGACUCGCCCGA 4617_H04
GATTT CCTCTTTTATCCG GGGAGGACGAUGCGGGAUUUCUCCUCUUUUAUCCGCAGACGACUCGCCCGA 4618J-i04
CTTTTCAATTCCTTTTACCC GGGAGGACGAUGCGGCU UUUCAAUUCCUUUUACCCCAGACGACUCGCCCGA 4619„H04
TTTTCATTTTATCCTCTGTG GGGAGGACGAUGCGGUUUUCAUUUUAUCCUCUGUGCAGACGACUCGCCCGA 4620..H04
TCTTTTTTTGTTTTTCTG CCT GGGAGGACGAUGCGGUCUUUUU UUGUUUUUCUGCCUCAGACGACUCGCCCGA 4621JH04
ACATCTTTTTTTCTTTTGCGC GGGAGGACGAUGCGGACAUCU UU UUUUCU UU UGCGCCAGACGACUCGCCCGA 4622_H04
TTTTCTCAATTTATTTCCCG GGGAGGACGAUGCGGUUUUCUCAAUUUAU UUCCCGCAGACGACUCGCCCGA 4623_H04
ATTTCATCTTTACTTCTCTG GGGAGGACGAUGCGGAUUUCAUCUUUACUUCUCUGCAGACGACUCGCCCGA 4624_H04
ATTTTCATTTCCCTTTACCG GGGAGGACGAUGCGGAU UU UCAUUUCCCUU UACCGCAGACGACUCGCCCGA 4625_HQ4
CTTTTTACTATCCTTTTCTG GGGAGCACGAUGCGGCUUUU UACUAUCCUU UUCUGCAGACGAC'JCGCCCGA 4626_H04
TTATTTCTTCT'ACCC CTCCG GGGAGGACGAUGCGGUUAUUUCUUCUACCCCUCCGCAGACGACUCGCCCGA 4627_ H04
TCTAATTTTTACCTCCTTCCG GGGAGGACGAUGCGGUCUAAUUUUUACCUCCUUCCGCAGACGACUCGCCCGA 4628_H04
CTTTCAACTTTTTTCCGCGT GGGAGGACGAUGCGGCUUUCAACUUU UUUCCGCGUCAGACGAC'JCGCCCGA 4629_H04
AT'f TATTTTTG CCCTTACCA GGGAG6ACGAUGCGGAUUUAUUUUUGCCCUUACCACAGACGACUCGCCC6A 4630_H04
TTTTTCTTTAATTTGTG CCC GGGAGGACGAUGCGGUUUUUCUUUAAUUUGUGCCCCAGACGACUCGCCCGA 4631_H04
CATTCACTATTCTTTTTCCG GGGAGGACGAUGCGGCAUUCACUAUUCUUUUUCCGCAGACGACUCGCCCGA 4632JH04
TTCTTATATTCCGCTTCCCG GGGAGGACGAUGCGGUUCUUAUAUUCCGCUUCCCGCAGACGACUCGCCCGA 4633 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc
TATTTTTCCTTTTCTGGCCC GGGAGGACGAUGCGGUAUUUUUCCUUUUCUGGCCCCAGACGACUCGCCCGA 4634_H04
GTTTCCTTTAGTCTTTTCCG GGGAGGACGAUGCGGG UUUCCUUUAGUCUUU UCCGCAGACGACUCGCCCGA 4635JH04
TTCATTTATTTTACTTTGCC GGGAGGACGAUGCGGU UCAUUUAUUUUACUU UGCCCAGACGACUCGCCCGA 4636_ H04
TATCTTG TTATTTCTTCCTG GGGAGGACGAUGCGGUAUCUUG UUAUUUCUUCCUGCAGACGACUCGCCCGA 4637_H04
TTTCTTTTTAGTTCTTTCCG GGGAGGACGAUGCGGU UUCUUUUUAGUUCUUUCCGCAGACGACUCGCCCGA 463S_H04
TTATATGTCTTTTTTTTGTCTG GGGAGGACGAUGCGGU UAUALJGUCUUUUUUUUGUCUGCAGACGACUCGCCCGA 4€39„H04
GACTT1 ACTCTTCTTTTCCC GGGAGGACGAUGCGGGACUU UACUCUUCUUUUCCCCAGACGACUCGCCCGA 4S40..H04
ATCTCTTTG CTCTTATACCG GGGAGGACGAUGCGGAUCUCUU UGCUCUUAUACCGCAGACGACUCGCCCGA 4641_M04
TTTACAATTTTCTCTTG CCG GGGAGGACGAUGCGGUUUACAAUU UUCUCUUGCCGCAGACGACUCGCCCGA 4642_H04
ACTTCTATTTCTCTCTTGCC GGGAGGACGAUGCGGACUUCUAUUUCUCUCUUGCCCAGACGACUCGCCCGA 4643__H04
TCTTTATTCTG TCTTCT CTG GGGAGGACGAUGCGGUCUU UAUUCUGUCUUCUCUGCAGACGACUCGCCCGA 4644_H04
CTTACTTATATCTTTCTCCG GGGAGGACGAUGCGGCUUACUUAUAUCU UUCUCCGCAGACGACUCGCCC A 4645_H04
o CCTTTTCATTTCTCTTCCTG GGGAGGACGAUGCGGCCUUUUCAUUUCUCUUCCUGCAGACGACUCGCCCGA 4646_H04
TATTT ATTTTTTCTTG CCCCT GGGAGGACGAUGC6GUAUUUAU UUUUUCUUGCCCCUCAGACGACUCGCCCGA 4647JH04
CTTTATTTTAATTCCCCCCG GGGAGGACGAUGCGGCU UUAUU UUAAUUCCCCCCGCAGACGACUCGCCCGA 4648 JH04
CATAGGAGTAGGGCGGTAGC GGGAGGACGAUGCGGCAUAGGAG UAGGGCGGUAGCCAGACGACUCGCCCGA 4649_H04
TTTTATGCTTTTACCTTCCG GGGAGGACGAUGCGGUUUUAUGCUUUUACCU UCCGCAGACGACUCGCCCGA 465O„H04
CTACATACTTTCTTCTTCCG GGGAGGACGAUGCGGCUACAUACU UUCUUCUUCCGCAGACGACUCGCCCGA 46S1_H04
CCTTTTGTAATCTTTCCG GGGAGGACGAUGCGGCCU UUUGUAAUCUUUCCGCAGACGACUCGCCCGA 4652_HQ4
G CCTTTTG TA ATTTTTTCCC GGGAGGACGAUGCGGGCCUU UUGUAAUUUUUUCCCCAGACGACUCGCCCGA 4653_H04
ATTTGTTATGTTTTTGTACCG GGGAGGACGAUGCGGAUiJUGUUAUGUUUUUGUACCGCAGACGACUCGCCCGA 4654_H04
TTTATTTATTTTACTCTCCA GGGAGGACGAUGCGGUUUAUUUAUUUUACUCUCCACAGACGACUCGCCCGA 4655_ H04
TCTTCACTTTTTATGTTCCG GGGAGGACGAUGCGGUCUUCACU UUUUAUGUUCCGCAGACGACUCGCCCGA 4656_,Η04
TTTCCCTTTTTGTTACTTGCCG GGGAGGACGAUGCGGUUUCCCUU UUUGUUACU UGCCGCAGACGACUCGCCCGA 4657_ H04
TTGTACTCTTCTCTTTCCCG GGGAGGACGAUGCGGUUGUACUCUUCUCUUUCCCGCAGACGACUCGCCCGA 4658_H04
ATCTATACTTACTCTCACCG GGGAGGACGAUGCGGAUCUAUACUUACUCUCACCGCAGACGACUCGCCCGA 4660 H04
# in H4
HTS read variable region A sequences # in H3 selection selection Selected sequenc TTTG TTT ATTCTTTTTTTTCCG GGGAGGACGAUGCGGU UUGUUUAUUCUUU UUUUUCCGCAGACGACUCGCCCGA 4661_H04
A TTCATCTTTCTTTTGCCA GGGAGGACGAUGCGGAUUUCAUCUUUCUUUUGCCACAGACGACUCGCCCGA 4662_H04
ATGTGGTGTTTGCTTAGCTG GGGAGGACGAUGCGGAUGUGGUG UU UGCUUAGCUGCAGACGACUCGCCCGA 4663„H04
TriTTTGATTTTCCGGTCTG GGGAGGACGAUGCGGU UUUUUGAUUUUCCGGUCUOCAGACGACUCGCCCGA 4664_H04
ATCTTTCACTATTCACTCCG GGGAGGACGAUGCGGAUCUUUCACUAUUCACUCCGCAGACGACUCGCCCGA 4665_H04
TCCTGTCGTCTGTTCTTCC GGGAGGACGAUGCGGUCCU6UCGUCUGUUCU UCCCAGACGACUCGCCCGA 4666_ H04
GCCTTTCGTCTCCCTCCC GGGAGGACGAUGCGGGCCUUUCGUCUCCCUCCCCAGACGACUCGCCCGA 4567_HG4
TGATTCCTCTGGTTCGTCCC GGGAGGACGAUGCGGUGAUUCCUCUGGUUCGUCCGCAGACGACUCGCCCGA 4668„HQ4
GCCTTTCGGTCTACCCTCCC GGGAGGACGAUGCGGGCCUU UCGGUCUACCCUCCCCAGACGACUCGCCCGA 4669_H04
GCCTTTCGTTCTG CACCTCCC GGGAGGACGAUGCGGGCCUU UCGUUCUGCACCUCCCCAGACGACUCGCCCGA 467CLH04
TTTG6AGGGTGCTGGGTCCG G6GAGGACGAUGCGGUUUGGAGGGUGCUGGGUCCGCAGACGACUCGCCCGA 4672_H04
CTTCATTCCTTTCACTACCG GGGAGGACGAUGCGGCU UCAUUCCUU'JCACUACCGCAGACGACUCGCCCGA 675_H04
o
•J\ TCTTTTTCTACCCTTTCCG GGGAGGACGAUGCGGUCUUUUUCUACCCUUUCCGCAGACGACUCGCCCGA 4676J G4
TAAGGAGGAGTTGGGGACCG GGGAGGACGAUGCGGUAAGGAGGAGUUGGGGACCGCAGACGACUCGCCCGA 4677_H04
TCCCTTTTACCTTTTCCCCG GGGAGGACGAUGCGGUCCCUU UUACCUUUUCCCCGCAGACGACUCGCCCGA 4678„H04
TTTTTTTCACTTTTCCCTCTG GGGAGGACGAUGCGGijUUU UUyCACUUU UCCCUCUGCAGACGACUCGCCCGA 4679_H04
TACTTTCACTTGC CTTTCCG GGGAGGACGAUGCGGUACUU UCACUUGCCUUUCCGCAGACGACUCGCCCGA 468D_H0
TGTTTTATAGTTCTGTTCCG GGGAGGACGAUGCGGUGUUUUAUAGUUCUG UUCCGCAGACGACUCGCCCGA 4681_H04
ACTTTTTTA ! I 1 1 i I'ACCTG GGGAGGACGAUGCGGACUUU UUUAUUUUUUACCUGCAGACGACUCGCCCGA 4S82_H04
TTCTTGTTTTCTCTATCCCC GGGAGGACGAUGCGGUUCUUGUUU UCUCUAUCCCCCAGACGACUCGCCCGA 4683_H04
ACTTTCCTTTGTTTC CACCG GGGAGGACGAUGCGGACUUUCCUUUGUUUCCACCGCAGACGAeUCGCCCGA; 4684_H04
CATTG7TCGTTTTTTCTCGC GGGAGGACGAUGCGGCAUUGUUCGUUUUUUCUCGCCAGACGACUCGCCCGA 4685_HG4
TTTCTCTTTTCTCTTTCC G GGGAGGACGAUGCGGUUUCUCUUUUCUCU UUCCGCAGACGACUCGCCCGA 4686_H04
CTCATTCTTATTTATTTCCG GGGAGGACGAUGCGGCUCAUUCUUAUUUAUUUCCGCAGACGACUCGCCCGA 4687_ H04
TATT HTTTGG TTCCA ACCTTTTTTTTTCCG GGGAGGACGAUGCGGUAUU UUGUCACUUUUUUUUCCGCAGACGACUCGCCCGA 46SS_H04
TFTGTTTTTCTTI rrrccG: GGGAGGACGAUGCGGUUUG UUU UUCUUUUUUUCCGCAGACGACUCGCCCGA 4689 H04
c cu
Figure imgf000307_0001
c 0 x
Figure imgf000307_0002
# in H4
HTS read variable region RNA sequences selection Selected sequenc
TCTAT ATTG ATCTCTCTCCG GGGAGGACGAUGCGGUCUAUAUUGAUCUCUCUCCGCAGACGACUCGCCCGA 4721_H04
GTTTGTC ! T 1 1 1 f 1 1 CGTCCG GGGAGGACGAUGCGGGUUUGUCUUUUUUUUCGUCCGCAGACGACUCGCCCGA 4723_H04
CCTTTTACTCTTTTTCTCCG GGGAGGACGAUGCGGCCUUUUACUCUUUUUCUCCGCAGACGACUCGCCCGA 4?24_H04
CTTTCACTTTTTCTCTCTCCG GGGAGGACGAUGCGGCUUUCACUUUUUCUCUCUCCGCAGACGACUCGCCCGA 472S_H04
TCTTT['TAACTTTTTTCTCCG G6GAGGACGAUGCGGUCUUUU UAACU UU UUUCUCCGCAGACGACUCGCCCGA 4726_H04
TTTTTGTCTTTTTTTACCCG G GGAGG ACG A UGCGG'J UU UUG UCUUUU UU UACCCGCAGACG ACUCGCCCGA 4727 H04
GCTATTCTnTCTTTTTCCCC GGGAGGACGAUGCGGGCUAUUCUUUUCUUUUUCCCCCAGACGACUCGCCCGA 4728_H04
TTTG A AG GTTT ATG G GTC C G GGGAGGACGAUGCGGU'JUGAAGGUUUAUGGGUCCGCAGACGACUCGCCCGA 4729JH04
CCTTTACTTTAATCArrCCCG GGGAGGACGAUGCGGCCUUUACUUUAAUCAUUCCCGCAGACGACUCGCCCGA 473Q.. H04
CTTCATITTATCTCTTTC CG GGGAGGACGAUGCGGCU UCAUU UUAUCUCUUUCCGCAGACGACUCGCCCGA 4731_H04
CTTTCACTTTTTTTTCTCCG GGGAGGACGAUGCGGCU UUCACU UU UUUUUCUCCGCAGACGACUCGCCCGA 4733JH04
TTTTTCATTTCCTTTTTCCG GGGAGGACGAUGCGGUUUUUCAUUUCCUUUUUCCGCAGACGACUCGCCCGA 4734_H04
CCATTTGTT I H i l l I CCTCCG GGGAGGACGAUGCGGCCAUUUGUUUUUUUUUCCUCCGCAGACGACUCGCCCGA 4735_HQ4
CTTGTTTTTCTCTTTTTCCG GGGAGGACGAUGCGGCUUGUUUUUCUCUUUUUCCGCAGACGACUCGCCCGA 4736_H04
GTTCTGTCGTCTGTTCGTCCC GGGAGGACGAUGCGGGUUCUGUCGUCUGUUCGUCCCCAGACGAC'JCGCCCGA 473S_H04
ATTCTTGTTTTTTTG CCG GGGAGGACGAU6CGGAU UCUUG UUUUUUUGCCGCAGACGACUCGCCCGA 4739_H04
TCCrrCCGTTCTGCCCTCCC GGGAGGACGAUGCGGUCCUUCCGUUCUGCCCUCCCCAGACGACUCGCCCGA 4740J-I04
ATTCACTCCTTTTCTTG CCG GGGAGGACGAUGCGGAUUCACUCCUUUUCUUGCCGCAGACGACUCGCCCGA 4741_H04
TACTCTTATTTTTACCTCCG GGGAGGACGAUGCGGUACUCUUAUUUUUACCUCCGCAGACGACUCGCCCGA 4742_H04
GCTTTCGTTCTACCCTCCC GGGAGGACGAUGCGGGCUUUCGUUCUACCCUCCCCAGACG ACUCGCCCGA 4743JH04
TTCATTTTCTTTTCTTCCG GGGAGGACGAUGCGGUUCAUUUUCU UUUCUUCCGCAGACGACUCGCCCGA 4744_H04
TCCTGTCGACTGCTCGTCCC GGGAGGACGAUGCGGUCCUGUCGACUGCUCGUCCCCAGACGACUCGCCCGA 4745_H04
GTCCTGTCGTCTCTTCGTCCC G6GAGGACGAUGCGGGUCCUGUCGUCUCUUCGUCCCCAGACG ACUCGCCCGA 4746_H04
G TCCTGTC G TCTGTACGTCCC GG6A6GACGAUGCGGGUCCUGUCGUCUGUAC6UCCCCAGACGACUCGCCCGA 4748_H04
G CCTiCG TTCTCCCTCCC GGGAGGACGAUGCGGGCCUUCGU UCUCCCUCCCCAGACGACUCGCCCGA 4749_H04
TCCTTATGTTTTACGTCCCC GGGAGGACGAUGCGGUCCUUAUGUUUUACG UCCCCCAGACGACUCGCCCGA 4750 H04
# in H4
HTS read variable region RNA sequences # H3 selection selection Selected sequenc TACGCGGTATAAGTGTGCCC GGGAGGACGAUGCGGUACGCGGUAUAAGUGUGCCCCAGACGACUCGCCCGA 4751_H04
TTCCTTTTGC TTCTCTCCG GGGAGGACGAUGCGGUUCCUU UUGCUUUCUCUCCGCAGACGACUCGCCCGA 4752_HQ4
TG ACTCGTCTGTTTGTCCC GGGAGGACGAUGCG6UGACUCGUCUGUU LJGUCCCCAGACGACUCGCCC6A 4753_H04
ATTCTTGCC i D i l i 1'GCCG GGGAGGACGAUGCGGAUUCUUGCCUUUUUUUGCCGCAGACGACUCGCCCGA 4754 .H04
TTTTAAGGGTGTTGGGTCCG GGGAGGACGAUGCGGUUUUAAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 4755_H04
GTTTTT6TCTTTCTCATCCC GGGAGGACGAUGCGGGUU UUUGUCUUUCUCAUCCCCAGACGACUCGCCCGA 4756„H04
TTC ATC ATTCATTTTCTG CC GGGAGGACGAUGCGGUUCAUCAUUCAUUUUCUGCCCAGACGACUCGCCCGA 4757_H04
TCCmCGTTCTG CCCTCC GGGAGGACGAUGCGGUCCUUUCGUUCUGCCCUCCCAGACGACUCGCCCGA 4758_H04
TTTCCTGCTTCTATTATCCG GGGA6GACGAUGCGGUUUCCUGCUUCUAUUAUCCGCAGACGACUCGCCCGA 4759_H04
ATTTGGTCTCTTTTITCGCCG GGGAGGACGAUGCGGAUU UGG UCUCUUUUU UCGCCGCAGACGACUCGCCCGA 4760_H04
TGTTTCCTTTTCCTTCGCCC GGGAGGACGAUGCGGUGUUUCCUUUUCCUUCGCCCCAGACGACUCGCCCGA 4761_H04
ATTTCTCTCTTCTTTGCCG GGGAGGACGAUGCGGAUUUCUCUCUUCUUUGCCGCAGACGACUCGCCCGA 762_H04
CATAGGGGTAGGGTGGTAGC GGGAGGACGAUGCGGCAUAGGGGUAGGGUGGUAGCCAGACGACUCGCCCGA 4765_H04
TCCTTGTCGTCTTGCGACCC GGGAGGACGAUGCGGUCCU UGUCGUCUUGCGACCCCAGACGACUCGCCCGA 767^ H04
GCTTTTCGTTCTGCCCCCCC GGGAGGACGAUGCGGGCUUUUCGUUCUGCCCCCCCCAGACGACUCGCCCGA 4768„H04
TCAC AGCG G AATTGTG G CCC GGGAGGACGAUGCGGUCACAGCG6AAUUGUGGCCCCAGACGACUCGCCCGA 4769.H04
ATTACTCTTTTCTTTTGCCG GGGAGGACGAUGCGGAUUACUCUUUUCUUUUGCCGCAGACGACUCGCCCGA 4770_H04
CTCGCTTTTCATCTTCTCCG GGGAGGACGAUGCGGCUCGCUU UUCAUCUUCUCCGCAGACGACUCGCCCGA 4771_H04
TCTCATCTTCTTTTCTCCG GGGAGGACGAUGCGGUCUCAUCUUCUUUUCUCCGCAGACGACUCGCCCGA 4772_H04
TCTTTTCATG CTTCTCTCCG GGGAGGACGAUGCGGUCUU UUCAUGCUUCUCUCCGCAGACGACUCGCCCGA 4774_H04
TCCTGTCGCCTGCTCGTCCC GGGAGGACGAUGCGGUCCUG UCGCCUGCUCGUCCCCAGACGACUCGCCCGA 4775_H04
ATTCTTTTCTCTTTTCTCCG GGGAGGACGAUGCGGAUUCUUUUCUCUUUUCUCCGCAGACGACUCGCCCGA 4776_H04
ACATTATTTTCTTCTCTGCC GGGAGGACGAUGCGGACAUUAU UUUCUUCUCUGCCCAGACGACUCGCCCGA 4778_H04
CCTATTCCTTACTCTCTCCG GGGAGGACGAUGCGGCCUAUUCCUUACUCUCUCCGCAGACGACUCGCCCGA 4779 H04
ATA CTTTCACTCTCTTCCCG GGGAGGACGAUGCGGAUACUUUCACUCUCU UCCCGCAGACGACUCGCCCGA 4780_HQ4
GCCTTTCGTTCTATCCTCCC GGGAGGACGAUGCGGGCCUUUCGUUCUAUCCUCCCCAGACGACUCGCCCGA 4781 H04
cr at
Figure imgf000310_0001
¾
Figure imgf000310_0002
# in H4
HTS read aria le region RNA sequences 5 selection selection Selected sequenc TTTGAAGGGTGTTGGTTCCG GGGAGGACGAUGCGGUUUGAAGGGUGUUGGUUCCGCAGACGACUCGCCCGA 4809_H04
TTCGAAG G GTGACG G GTCCG GGGAGGACGAUGCGGUUCGAAGGGUGACGGGUCCGCAGACGACUCGCCCGA 4810_H04
TTAGGGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUAGGGAGUAGGGUGUUCCGCAGACGACUCG'CCCG'A 4812__HG4
TTTTTGTTTTATTGGTTCCG GGGAGGACGAUGCGGUUUUUGUU UUAUUGGUUCCGCAGACGACUCGCCCGA 4813 JH04
GCCTTTCGTTCTACCCTCCG GGGAGGACGAUGCGGG CUU UCGUUCUACCCUCCGCAGACGACUCGCCCGA 4814_H04
ATTCTTTTCTCTTTC CA CCG GGGAGGACGAUGCGGAU UCUUUUCUCX'UUCCACCGCAGACGACUCGCCCGA 4S15_HQ4
TTTGAAGGGTGTTAGGTCCG GGGAGGACGAUGCGGUUUGAAGGG UGUUAGGUCCGCAGACGACJCGCCCGA 4816_H04
TTGGGTGCAGGGCGGTACCG GGGAGGACGAUGCGGU UGGGUGCAGGGCGGUACCGCAGACGACUCGCCCGA 4817JH04
TTTGAGGGGAGGTGGGTCCG GGGAGGACGAUGCGGUUUGAGGGGAGGUGGGUCCGCAGACGACUCGCCCGA 4818_H04
GATATTTACTTTTT TGGCC GGGAGGACGAUGCGGGAUAUUUACUUUU UUUGGCCCAGACGACUCGCCCGA 4819_H04
TTTG ACG G G TGTTG GGTCCG GGGAGGACGAUGCGGU UUGACGGGUGUUGGGUCCGCAGACGACUCGCCCGA 4820_H04
TCTG TTTTTCTCTTTTTCCG GGGAGGACGAUGCGGUCUG UUUUUCUCUUUUUCCGCAGACGACUCGCCCGA 4821„H04
ACATTTCATTTTTTTCCCCCG GGGAGGACGAUGCGGACAUUUCAUUUUUU UCCCCCGCAGACGACUCGCCCGA 4822^H04
TTCTTA ! I Π Ι CCCTCCG GGGAGGACGAUGCGGUUCUUAU UUU UUCCCUCCGCAGACGACUCGCCCGA 4824_ H04
CCTTTTTATGTTCTTTGCG GGGAGGACGAUGCGGCCUUUUUAUGU UCU UUCCGCAGACGACUCGCCCGA 4825_H04
CTTCTTGTGTTTTTTTGCTG GGGAGGACGAUGCGGCUUCUUGUGUUIJUUU UGCUGCAGACGACUCGCCCGA 4826_H04
TTCTTCTTCTTTTCCTCCG GGGAGGACGAUGCGGUUCUUCUUCU UUUCCUCCGCAGACGACUCGCCCGA 4827_H04
TTTG A AGTGTTATG G GTCCG GGGAGGACGAUGCGGUU UGAAG UGU UAUGGGUCCGCAGACGACUCGCCCGA 4828_H04
GTTCTTAATCACCTTTTCCC GGGAGGACGAUGCGGGU UCUUAAUCACCUU UUCCCCAGACGACUCGCCCGA 4829_H04
TGTATTATTATTTGTTGCTG GGGAGGACGAUGCG6UGUAU UAUUAUUUGUUGCUGCAGACGACUCGCCCGA 4830J4Q4
CTTTATTTTrGTTTTTTG CC GGGAGGACGAUGCGGCUUUAUUUUUGUUUUUUGCCCAGACGACUCGCCCGA 4S31JHQ4
CCATATTTCTTCTTTATCCC GGGAGGACGAUGCGGCCAUAUUUCUUCUUUAUCCCCAGACGACUCGCCCGA 4832 H04
I I I i I I CTCCGGTTTTCCG GGGAGGACGAUGCGGUUUUUUCUCCGGUU UUCCGCAGACGACUCGCCCGA 4S33_H04
TATTCTTCATTATTTG TCAC GGGAGGACGAUGCGGUAUUCUUCAUUAUUUGUCACCAGACGACUCGCCCGA 4834_H04
CCTTTTTTTGTCCTTTGCCC GGGAGGACGAUGCGGCCU UUUUUUGUCCUUUGCCCCAGACGACUCGCCCGA 4S35_H04
CTCATTTATTACTTTTACCG GGGAGGACGAUGCGGCUCAUUUAUUACUUUUACCGCAGACGACUCGCCCGA 4836 H04
» in H4
HTS read variable region RNA sequences 3 selection selection Selected sequenc ATTTTATCTATCCGTTTCCG GGGAGGACGAUGCGGAUUUUAUCUAUCCGUUUCCGCAGACGf¾CUCGCCCGA 4837 H04
CTTTCACGTCATCTTTTCC G GGGAGGACGAUGCGGCUUUCACGUCAUCUUUUCCGCAGACGACUCGCCCGA 48B8 H04
- GCTTTACGTTCTTTATTCCG GGGAGGACGAUGCGGGCUU UACGliUCUUUAUUCCGCAGACGACUCGCCCGA 4839_K04
TCACATTTAC f 1 1 1 1 1 I GCG GGGAGGACGAUGCGGUCACAUUUACUUUUUUUGCGCAGACGACUCGCGCGA 4840_H04
CTTGTTG TTTTTTTTATCCC GGGAGGACGAUGCGGCUUGUUGUUUUUUUUAUCCCCAGACGACUCGCCCGA 4841JHG4
TTCCTTTTTACTTTCTTGTG GGGAGGACGAUGCGGU UCCUUOUUACUUUCUUGUGCAGACGACUCGCCCGA 4842_'H04
TTTTCTCTTCTCTACTTCTG GGGAGGACGAUGCGGUUUUCUCUUCUCUACUUCUGCAGACGACUCGCCCGA 4843_H04
CTTCACTTATTCCTTATCCC GGGAGGACGAUGCGGCUUCACUUAUUCCUUAUCCCCAGACGACUCGCCCGA 4S44_H04
ATTTTTACATTTGCTCTCCG GGGAGGACGAUGCGGAUUU UUACAUU UGCUCUCCGCAGACGACUCGCCCGA 4S45_H04
TTATCTATATTCTCTCTGTG GGGAGGACGAUGCGGUUAUCUAUAUUCUCUCUGUGCAGAC6ACUCGCCCGA 4846_H04
TCTTTTCATTTTTCTCTCCG GGGAGGACGAUGCGGUCUUUUCAUUUUUCUCUCCGCAGACGACUCGCCCGA 484?_H04
TTTTTTTACTTCTCT7CGCTG GGGAGGACGAUGCGGUUUUUUUACUUCUCUUCGCUGCAGACGACUCGCCCGA 4848_ H04
CTTTTACTGTTTCCTTTTCCG GGGAGGACGAUGCGGCUUUUACUGUUUCCUUUUCCGCAGACGACUCGCCCGA 4849_H04
ATTTGTTCTTTAATTTGCCG GGGAGGACGAUGCGGAU UUGUUCUUUAAUU UGCCGCAGACGACUCGCCCGA 4850„H04
TTCTTTATTTTCTCCCTCCG GGGAGGACGAUGCGGUUCUUUAUUUUCUCCCUCCGCAGACGACUCGCCCGA 4851_H04
CTTTCCAGGCTTTTTCTCCG GGGAGGACGAUGCGGCU UUCCAGGCU UUUUCUCCGCAGACGACUCGCCCGA 4852JH04
TCA6TTATTATTTTTTTCGC GGGAGGACGAUGCGGUCAGUUAUUAUUUUUUUCGCCAGACGACUCGCCCGA 4853_H04
TATG A AG GTG AG GTG GTCCG GGGAGGACGAUGCGGUAUGAAGGUGAGGUGGUCCGCAGACGACUCGCCCGA 485 JH04
CTTAATCTTTCTTCCTTCCG GGGAGGACGAUGCGGCUUAAUCUUUCUUCCUUCCGCAGACGACUCGCCCGA 485S_H04
ACTCTTATCTTATCTT6CCG GGGAGGACGAUGCGGACUCUUAUCUUAUCU UGCCGCAGACGACUCGCCCGA 4856_H04
TTTTCTTTTTCACTTTTCCG GGGAGGACGAUGCGGUUUUCUUUUUCACU UUUCCGCAGACGACUCGCCCGA 4857_H04
TCATTATCTTTCTTCTCCAC GGGAGGACGAUGCGGUCAUUAUCUUUCUUCUCCACCAGACGACUCGCCCGA 4858_H04
AATTA I I i I i I GCCTCTCCG GGGAGGACGAUGCGGAAUUAUU UUUUGCCUCUCCGCAGACGACUCGCCCGA 4859_H04
CTCTTTTTG GTTACTCTCCG GGGAGGACGAUGCGGCUCUUUUUGGUUACUCUCCGCAGACGACUCGCCCGA 4860 H04
TTTACTCTTACCTTTGCCCG GGGAGGACGAUGCGGUU UACUCUUACCUUUGCCCGCAGACGACUCGCCCGA 4861 H04
GTCCTTTTACTTTTTCCCCG GGGAGGACGAUGCGGGUCCUUUUACUUUUUCCCCGCAGACGACUCGCCCGA 4862 H04
# in H4
HTS read variable region NA sequences selection Selected sequenc GCTTCTTTTCTCTTTTCCC GGGAGGACGAUGCGGGCUUCU UUUCUCUUU UCCCCAGACGACUCGCCCGA 4863_H04
GTATCTTTTTCCTCGCTCCG GGGAGGACGAUGCGGGUAUCUUU UUCCUCGCUCCGCAGACGACUCGCCCGA 4864_H0
ACCTATTCTTTTACCCTACCG GGGAGGACGAUGCGGACCUAUUCUUUUACCCUACCGCAGACGACUCGCCCGA 4865_HQ4
ΤΑΤΤΤΑΪ Π T i ί Ί Ί CTCTCTG GGGAGGACGAUGCGGUAUUUAUUUUUUUUCUCUCUGCAGACGACUCGCCCGA 4866_H04
TCCCTTTGTTCTTTTCACCG GGGAGGACGAUGCGGUCCCU UUGUUCUU UUCACCGCAGACGACUCGCXCGA 4867_H04
TGTT ATTTTATCTGTTCC GGGAGGACGAUGCGGUGUUUUAUUU UAUCUGUUCCCAGACGACUCGCCCGA 4868_H04
c iTCA CTTCTATTTCCC GGGAGGACGAUGCGGCUUUUUCACUUCUAUUUCCCCAGACGACUCGCCCGA 4869_H04
CACTTTTGCTCT TTCTCGC GGGAGGACGAUGCGGCACUUUUGCUCUUUUCUCGCCAGACGACUCGCCCGA 4870_H04
CC ACTTTT A ATTTTCTCCG C GGGAGGACGAUGCGGCCACUUU UAAU UUUCUCCGCCAGACGACUCGCCCGA 4871_ H04
TTTCACTATCTTACCTTCCG CGGAGGACGAUGCGGUUUCACUAUCUUACCUUCCGCAGACGACUCGCCCGA 4872_H04
TTTG A ATG G 7GTTG GG TCCG GGGAGGACGAUGCGGUUUGAAUGGUGUUGGGUCCGCAGACGACUCGCC GA 4873_H04
TTTCTACTTTTTCATTGTCCG GGGAGGACGAUGCGGU UUeUACUUUUUCAUUGUCCGCAGACGACUCGCCCGA 4874_H04
TTTGAT6GTTGATGGGTCCG GGGAGGACGAUGCGGU UUGAU6GUUGAUGGGUCCGCAGACGACUCGCCCGA 4875_H04
TTTATCACTTTTCTTTGCAC GGGAGGACGAUGCGGU UUAUCACUUUUCUUUGCACCAGACGACUCGCCCGA 4876_H04
ACTTTGTCATCCTTTTG CCG GGGAGGACGAUGCGGACUUUGUCAUCCUU UUGCCGCAGACGACUCGCCCGA 4877„H04
CCTTCACTTTTTCTTTTCCG GGGAGGACGAUGCGGCCUUCACUUUUUCUUUUCCGCAGACGACUCGCCCGA 487g_ H04
CTTTCATTCACATTCCTCCG GGGAGGACGAUGCGGCUUUCAUUCACAU UCCUCCGCAGACGACUCGCCCGA 4879_H04
CTTTTTCAGTCTTGCTTCCG GGGAGGACGAUGCGGCUUUUUCAGUCUUGCU UCCGCAGACGACUCGCCCG 4880_H04
GATTTGTTTATGTTTACCTG GGGAGGACGAUGCGGGAUU UGUU UAUGUUUACCUGCAGAC6ACUCGCCCGA 4881_H04
TTTCCGTTCTATTTTCTG CC GGGAGGACGAUGCGGUUUCCGU UCUAUUUUCUGCCCAGACGACUCGCCCGA 4882_ HG4
CTTTTTTTTCCT TTTCCG GGGAGGACGAUGCGGCUUUUU UUUCCUUUU UUCCGCAGACGACUCGCCCGA 4884_H04
TTATTTATTTCT rTGTGTG G6GAGGACGAUGCGGUUAUUUAUUUCUUUUGUGUGCAGACGACUCGCCCGA 4885_H04
CATTCG'ITTACTTTTTCCGG GGGAGGACGAUGCGGCAUUGGUUUACUUUUUCCCGCAGACGACUCGCCCGA 4886_H04
AA AG GAG GAG CTG GG ATCTG GGGAGGACGAUGCGGAAAGGAGGAGCUGGGAUCL'GCAGACGACUCGCCCGA 4887_H04
GTTGGGGTAGGGGGGTTCCG GGGAGGACGAUGCGGG UUGGGGUAGGGGGGUUCCGCAGACGACUCGCCCGA 4888_HQ4
CTTTTCACGGCTTTTTTCCG GGGAGGACGAUGCGGCUUU UCACGGCUUU UUUCCGCAGACGACUCGCCCGA 4889 H04
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# in H4
HTS read variable region RNA sequences i3 selection selection Selected sequenc CTTAGGAGTAGGGTGGTAGC GGGAGGACGAUGCGGCU UAGGAGUAGGGUGGUAGCGAGACGACUCGCCCGA 4952JH04
TATTTTACTAACCTTTTTCCG GGGAGGACGAUGCGGUAUUUUACUAACCUUUU UCCGCAGACGACUCGCCCGA 4953_H04
TTTCTTTCGTTTTTCTCCG GGGAGGACGAUGCGGU UUCUUUCGUUUUUCUCCGCAGACGACUCGCCCGA 4954_H04
TTATTTAAATTTTTTGTTGTG GGGAGGACGAUGCGGUUAU UUAAAUUUUUUGUUGU6CAGACGACUCGCCCGA 4955_H04
GGGAGGACGAUGCGGUAUCCUU UUUCUUUUU UGCCCAGACGACUCGCCCGA 4956_H04
ACCTTTAACGTTTCATTCCG GGGAGGACGAUGCGGACCUU UAACG UUUCAUUCCGCAGACGACUCGCCCGA 4957_ H04
TTCTTTG ! ! 1 TTTTTCTCCCG GGGAGGACGAUGCGGUUOJUUGUUUUUUUUCUCCCGCAGACGACUCGCCCGA 4958_H04
TTTGTTTATTTTTTTrCCG GGGAGGACGAUGCGGUUUGUUUAUUUUUUUUCCGCAGACGACUCGCCCGA 4959_HG4
TACCTATTCTCGTTTTTTTGCC GGGAGGACGAUGCGGUACCUAUUCUCGUUUUUUUGCCCAGACGACUCGCCCGA 4960_H0
TTTTTATGTTTTCTCGTCGC GGGAGGACGAUGCGGUUUUUAUGUUU UCUCGUCGCGAGACGACUCGCCCGA 4962_H04
TTTTGTTTTTTT!TTCTGCC GGGAGGACGAUGCGGUUUUGU UUUUUUUUUCUGCCCAGACGACUCGCCCGA 49S3_H04
TCTTATTTTTCTCTCTCCG GGGAGGACGAUGCGGUCUUAUUUUUCUCUCUCCGCAGACGACUCGCCCGA 4964_H04
■ ■ I TGAAGGGCCATGGGTCCG GGGAGGACGAUGCGGUUUGAAGGGCCAUGGGUCCGCAGACGACUCGCCCGA 4966_H04
CCTTTACTTTTTTTCTCCG GGGAGGACGAUGCGGCCUUUACUUU UUUUCUCCGCAGACGACUCGCCCGA 4967_H04
CCATTTAATTTTTTCTCCCG GGGAGGACGAUGCGGCCAUUUAAUUUUUUCUCCCGCAGACGACUCGCCCGA 4969_H04
TATTTTCTCTTTACTTTTCCC GGGAGGACGAUGCGGUAUUUUCUCUUUACUUUUCCCCAGACGACUCGCCCGA 4970_H04
TTTTATTTTCTCTTCATTG G GGGAGGACGAUGCGGUUUUAUUUUCUCUUCAUUGGCAGACGACUCGCCCGA 49 1..H04
TTCTTTCTT CTTTTCTCCG GGGAGGACGAUGCGGUUCUU UCUUCUUUUCUCCGCAGACGACUCGCCCGA 4973_H04
TTTGAAGGGTAATGGGCCCG GGGAGGACGAUGCGGUU UGAAGGGUAAUGGGCCCGCAGACGACUCGCCCGA 4974_ H04
TTAGAAGGGCGATGGGTCCG GGGAGGACGAUGCGGUUAGAAGGGCGAUGGGUCCGCAGACGACUCGCCCGA 4975„H04
TTTGCGGGGTGATGGGTCCG GGGAGGACGAUGCGGUU UGCGGGGUGAUGGG UCCGCAGACGACUCGCCCGA 4976_H04
TCTATTCCTCCCTGCCGCCC GGGAGGACGAUGCGGUCUAUUCCUCCCUGCCGCCCCAGACGACUCGCGCGA 4977 H04
TTCATTTCTTTCCACGTTCCG GGGAGGACGAUG CGG UUCAUUUCUU UCCACG U UCCGCAGACG ACU CGCCCG A 497g_HG4
TTTG G G AG TAG G G GTTCCG GG6AGGACGAUGCGGUUUGGGAGUAGGGGUUCCGCAGACGACUCGCCCGA 4979_H04
ATTCTCTTTCTTTTCCACCG GGGAGGACGAUGCGGAUUCUCUU UCUUUUCCACCGCAGACGACUCGCCCGA 4981 H04
ACATG ACTG ATC AT6TGCCC GGGAGGACGAUGCGGACAUGACUGAUCAUGUGCCCCAGACGACUCGCCCGA 4982 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc TCCTTTCGTTCTGCGCTCCC GGGAGGACGAUGCGGUCCU UUCGUUCUGCGCUCCCCAGACGACUCGCCCGA 4983_H04
TCCTGTCGTCTGTTCTACCC GGGAGGACGAUGCGGUCCUGUCGUCUGUUCUACCCCAGACGACUCGCCCGA 4984_H04
TTTGGGAGTAGGGTGGTACCT GGGAGGACGAUGCGGUUUGGGAG UAGGGUGGUACCUCAGACGACUCGCCCGA 4985_MQ4
TCCTTTCGTTCTG CCCG CCC GGGAGGACGAUGCGGUCCU UUCGUUCUGCCCGCCCCAGACGACUCGCCCGA 4986_H04
TCCTGTCGGCTATTC6TCCC GGGAGGACGAUGCGGUCCUGUCGGCUAUUCGUCCCCAGACGACUCGCCCGA 49S7_ HQ4
GTTGTTTTACCTCCCCGCCC GGGAGGACGAUGCGGG UUGUU UUACCUCCCCGCCCCAGACGACUCGCCCGA 4988_ HG4
GCCTATTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCUAUUCGUUCUGCCeuCCCCAGACGACUCGCCCGA 4S89_H04
CCCACTTCTTCTCTATTCCG GGGAGGACGAUGCGGCCCACUUCUUCUCUAUUCCGCAGACGACUCGCCCGA 4990JH04
ATTTATTACCCCTTTTGCCC GGGAGGACGAUGCGGAUUUAUUACCCCUUUUGCCCCAGACGACUCGCCCGA 4991_HG4
TCTTTTTAACCTfTTCTCCG GGGAGGACGAUGCGGUCUU UUUAACCUUUIJCUCCGCAGACGACUCGCCCGA 4992„H04
TATTCTCTTGCTTTTCTCCG GGGAGGACGAUGCGGUAUUCUCUUGCUUUUCUCCGCAGACGACUCGCCCGA 4993_ H0
cATTCTcrrmTrmGccc GGGAGGACGAUGCGGCAUUCUCUUUU UU UCUUGCCCCAGACGACUCGCCCGA 4994_H04
ATTCACTTCTTTTTCTTGCCG GGGAGGACGAUGCGGAUUCACUUCUUUUUCUUGCCGCAGACGACUCGCCCGA 4996_ H04
GCTTTTCGTTGCCCTCCC GGGAGGACGAUGCGGGCU'JUUCGU UGCCCUCCCCAGACGACUCGCCCGA 4998_H04
ACATG TAATACCAGTGG CCC GGGAGGACGAUGCGGACAUG UAAUACCAGUGGCCCCAGACGACUCGCCCGA 4999_H04
ATTTCTCTTTCTTTGCCG GGGAGGACGAUGCGGAUUUCUCUUUCUUUGCCGCAGACGACUCGCCCGA 5000_H04
CCTTTCG'tTCTGCCCCCCC GGGAGGACGAUGCGGCCUUUCGUUCUGCCCCCCCCAGACGACUCGCCCGA 5001_H04
TCCTTTCGATCTGCCCTCCC GGGAGGACGAUGCGGUCCUUUCGAUCUGCCCUCCCCAGACGACUCGCCCGA 5002_H04
GCTTTCGTTCTGCTCTGCC GGGAGGACGAUGCGGGCUUUCGUUCUGCUCUCCCCAGACGACUCGCCCGA 5003__HQ4
TCCTTATGTTCCTCCTGCC GGGAGGACGAUGCGGUCCUUAUGUUCCUCCUGCCCAGACGACUCGCCCGA 5006_H04
TCTGAAG G GTG TTGG GTCCG GGGA6GACGAUGCGGUCUGAAGGGUGUUGGGUCCGCAGACGACUCGCCCGA 5007..H04
AAGATGAGGTGGCTCGTGCC G6GAGGACGAUGCGGAAGAUGAGGUGGCUCGUGCCCAGACGACUCGCCCGA 5008_H04
TCTAACGTTCCTTCCGTCCC GGGAGGACGAUGCGGUCUAACGUUCCUUCCGUCCCCAGACGACUCGCCCGA 5009_H04
TTGAAGTGTGATGGGTCCG GGGAGGACGAUGCGGU UGAAGUGUGAUGGGUCCGCAGACGACUCGCCCGA 5010_ H04
TTCTTCAAATGTTTTCCCCG GGGAGGACGAUGCGGU UCUUCAAAUGUUU UCCCCGCAGACGACUCGCCCGA 5011_H04
TATTTTCTTCCTTTTCTCCG GGGAGGACGAUGCGGUAUUUUCUUCCUUUUCUCCGCAGACGACUCGCCCGA SOI 2 H04
# irt H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc GCCTTTATTTTCCATCTCCG GGGAGGACGAUGCGGGGCU UUAUU UUCCAUCUCCGCAGACGACUCGCCCGA 5013_H04
TTTGAAGGGTGTTGGCTCCG 6GGAGGACGAUGCGGUUUGAAGGGUGUUGGCUCCGCAGACGACUCGCCCGA 5O14_H04
ATTATCTTTTCCTTG TTCCG GGGAGGACGAUGCGGAUUAUCUUUUCCUUGUUCCGCAGACGACUCGCCCGA 5015_ HQ4
TT7GATGG6TGCTGGGTCCG GGGAGGACGAUGCGGUUUGAUGGGUGCUGGGUCCGCAGACGACUCGCCCGA 50i6_ H04
TCACACCTTTTCTCTATCCG GGGAGGACGAUGCGGUCACACCUUU UCUCUAUCCGCAGACGACUCGCCCGA 5017_H04
TCTTATTCTTCTCTTCTCCG GGGAGGACGAUGCGGUCUUAUUCU UCUCUUCUCCGCAGACGACUCGCCCGA 5018_H04
CCTTTCTTCCACTTTTTGCC GGGAGGACGAUGCGGCCUUUCUUCCACUUUUUGCCCAGACGACUCGCCCGA 5019_HQ4
TATTT A CTTCTTTTTG TCCG GGGAGGACGAUGCGGUAU UUACUUCUUUUUGUCCGCAGACGACUCGCCCGA 5020_HG4
TGTTTTCTTCTTCTACTGCC GGGAGGACGAUGCGGUGUUUUCU UCUUCUACUGCCCAGACGACUCGCCCGA 5021_H04
TTATGTTTGTCCTTTTTCCG GGGAGGACGAUGCGGU UAUGU UUG UCCUUUUUCCGCAGACGACUCGCCCGA 5Q22_HQ4
TTCG AAAG GTG ATGG GTCCG GGGAGGACGAUGCGGU UCGAAAGG UGAUGGGUCC6CAGACGACUCGCCCGA S023_HG4
TTCTTACC GTCTTCTTG CCG GGGAGGACGAUGCGGU UCUUACCG UCUUCUUGCCGCAGACGACUCGCCCGA 5Q24„H04
ACCTTTTTGTTCGCTTTCCG GGGAGGACGAUGCGGACCUU UUUGUUCGCUUUCCGCAGACGACUCGCCCGA 5025_H04
GCCTTTCTTTCTACCCTCCC GGGAGGACGAUGCGGGCCUUUCUUUCUACCCUCCCCAGACGACUCGCCCGA 5026_H04
TCTTATCTATTTTCCCGCC C GGGAGGACGAUGCGGUCUUAUCUAUUUUCCCGCCCCAGACGACUCGCCCGA 5027_ H04
G C CTTTC GTTCTTCCCTTC c GGGAGGACGAUGCGGGCCUUUCGUUCUUCCCUUCCCAGACGACUCGCCCGA 5Q28_H04
CATTTTCCTTATTTTCGG CC GGGAGGACGAUGCGGCAUUUUCCUUAUUU UCGGCCCAGACGACUCGCCCGA SQ29_H04
ATG GTGTGTTTTATG CGGCTG GGGAGGACGAUGCGGAUGGUG UGUUUUAUGCGGCUGCAGACGACUCGCCCGA 5030„Η04
GTTGGTGTAGGAGGGTTCCG GGGAGGACGAUGCGGGUUGGUGUAGGAGGG UUCCGCAGACGACUCGCCCGA 5031_H04
CTTATGGAGTAGGGAGGGGC GGGAGGACGAUGCGGCUUAUGGAGUAGGGAGGGGCCAGACGACUCGCCCGA 5032_HO4
CTGTTTTTCCTGTTTGTCCC GGGAGGACGAUGCGGCUGU UUUUCCUGUUUGUCCCCAGACGACUCGCCCGA 5D33_ H04
TCCTGGCTCGTCGTTGTCCC GGGAGGACGAUGCGGUCCUGGCUCGUCGUUGUCCCCAGACGACUCGCCCGA S034_H04
GCCTTTCGTTCTGCCCTGCCC GGGAGGACGAUGCGGGCCUU UCGU UCUGCCCUGCCCCAGACGACUCGCCCGA 5035_H04
ACAGTGTAATGATCTGTGCC GGGAGGACGA'JGCGGACAGUGUAAUGAUCUGUGCCCAGACGACUCGCCCGA 5036_K04
TTTGAACGGTGATGGGCCCG GGGAGGACGAUGCGGU UUGAACGGUGAUGGGCCCGCAGACGACUCGCCCGA 5037J4O4
TTTG GGGGGAGGTGGGTCCG GGGAGGACGAUGCGGUUUG6GGGGAGGUGGGUCCGCAGACGACUCGCCCGA 5039 H04
HTS read variable region NA sequences # in H3 selection selection Selected sequenc TTTACTTTTTCTCCATGCCC GGGAGGACGAUGCGGUUUACU UU UUCUCCAUGCCCCAGACGACUCGCCCGA 5040, H04
TTTACTCGTTATTTTTTTG CCG GGGAGGACGAUGCGGUUUACUCGU UAUUUUUUUGCCGCAGACGACUCGCCCGA 5041_ _H04
TCTACATTTCTCTTCTTG C C GGGAGGACGAUGCGGUCUACAU UUCUCUUCUUGCCCAGACGACUCGCCCGA 5042. .H04
TCCTTGCTCGTCTTACACTG GGGAGGACGAUGCGGUCCUUGCUCGUCUUACACUGCAGACGACUCGCCCGA 5043. _HG4
AATTTCTCG ! I Π f l'GCTG GGGAGGACGAUGCGGAAUUUCUCGUUUUUUGCUGCAGACGACUCGCCCGA 5044_ _H04
TTTTCATCATATTTCTCCG C GGGAGGACGAUGCGGU UUUCAUCAUAUU UCUCCGCCAGACGACUCGCCCGA 5045. .H04
TTG TTTTTCTCTTTTTCCG GGGAGGACGAUGCGGU UGUUUUUCUCUUU UUCCGCAGACGACUCGCCCGA 5046. .H04
TTGTTTTCAGTGTTTGTCCG GGGAGGACGAUGCGGU UGUUUUCAGUGUUUGUCCGCAGACGACUCGCCCGA 5047. _H04
ATTATCTTTTCCACTTACCC GGGAGGACGAUGCGGAUUAUCU UUUCCACUUACCCCAGACGACUCGCCCGA 504S. H04
G ACTTTTT AATTCCTTAC C C GGGAGGACGAUGCGGGACU UUUUAAUUCCUUACCCCAGACGACUCGCCCGA 5Q49. „H04
TCTCTTTATTTTTTTTCTTCCG GGGAGGACGAUGCGGUCUCUUUAU UUUUUUUCUUCCGCAGACGACUCGCCCGA 5050. _H04
GAGTTTGTATGTTTTTGCCC GGGAGGACGAUGCGGGAGUUUGUAUG ULIUUU6CCCCAGACGACUCGCCCGA 5051. _H04
CTTTTTGCTCTTTTTTCTCCG GGGAGGACGAUGCGGCUUUUUGCUCUUUUUUCUCCGCAGACGACUCGCCCGA 5052. _H04
AATTTTATTTTACCTCACTG GGGAGGACGAUGCGGAAUUUUAUU UUACCUCACUGCAGACGACUCGCCCGA 5053. _H04
CCATTTTACGTTTACTTCCG GGGAGGACGAUGCGGCCAUU UUACGUUUACUUCCGCAGACGACUCGCCCGA 5054. _H04
CTTATATATCACTTTCTCCG GGGAGGACGAUGCGGCUUAUAUAUCACUUUCUCCGCAGACGACUCGCCCGA 5055. H04
AATTTTTCACTTTTTAACCC GGGAGGACGAUGCGGAAUUUUUCACUUUUUAACCCCAGACGACUCGCCCGA 5056. „H04
AGTATTTTTTCTCCGTTTCCG GGGAGGACGAUGCGGAGUAUUUUUUCUCCGU UUCCGCAGACGACUCGCCCGA 5057. .H04
TTTTCTACGTTGCTTTTCCC GGGAGGACGAUGCGGU UUUCUACGUUGCU UUUCCCCAGACGAr.UCGCCCGA 5058. .H04
CTTTTTTTATTCGTCTCCG GGGAGGACGAUGCGGCU UUUUUUAUUCG UCUCCGCAGACGACUCGCCCGA 5059. _H04
CTCTTTTCAATGTTTTACCG GGGAGGACGAUGCGGCUCUUUUCAAUGUUUUACCGCAGACGACUCGCCCGA 5060. .H04
GTCTTTCTTTCATTTTTCCC GGGAGGACGAUGCGGGUCUUUCUUUCAUUUUUCCCCAGACGACUCGCCCGA 5062. _H04
CTTTGCT TATCTTCTTCCG GG6AGGACGAUGCGGCUUUGCUUUAUCUUCUUCCGCAGACGACUCGCCCGA 5063. .H04
TGTTTGTTTTTTT GTG GGGAGGACGAUGCGGUGUUUG UUUUUUUUUG UGCAGACGACUCGCCCGA 5064. .H04
TTGTCTATTCrCTTTCGCCG GGGAGGACGAUGCGG UUGUCUAUUCUCUUUCGCCGCAGACGACUGGCCCGA 5065. .H04
ACTTTTCTCTACTTTCACCG GGGAGGACGAUGCGGACUUUUCUCUACUUUCACCGCAGACGACUCGCCCGA 5066. .H04
# irt H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc GAAC TTATTCTTTTTCG CCC GGGAGGACGAUGCGGGAACUUAUUCU UUUUCGCCCCAGACGACUCGCCCGA 5067_H04
ATCTTATGCTATTTTCTCCG GGGAGGACGAUGCGGAUCUUAUGCUAUUUUCUCCGCAGACGACUCGCCCGA 5068_H04
TTTGTAGGGTGCTGGGTCCG GGGAGGACGAUGCGGU UUGUAGGGUGCUGGGUCCGCAGACGACUCGCCCGA 5069_H04
GTATGTTTTTATTTGTTCCG GGGAGGACGAUGCGGG UAUGUUUUUAUUUGUUCCGCAGACGACUCGCCCGA 5070_H04
ATTTGTTTATTCTCCTTCCG GGGAGGACGAUGCGGAUUUGUUUAUUC'JCCU UCCGCAGACGACUCGCCCGA S071_H04
T nTTCATTTTACCGTTCCG GGGAGGACGAUGCGGUUUUUUCAUUUUACCG UUCCGCAGACGACUCGCCCGA S072_H04
CATCTTATTCTTTCCAACCG GGGAGGACGAUGCGGCAUCUUAUUCUUUCCAACCGCAGACGACUCGCCCGA 5073_HO4
AGTTTACTATCTCTGTTTCCG GGGAGGACGAUGCGGAGUUUACUAUCUCUGUUUCCGCAGACGACUCGCCCGA 5074..H04
TTGTTTTTGTTTGTGTCCCG GGGAGGACGAUGCGGUUGUUUUUGUUUGUGUCCCGCAGACGACUCGCCCGA 5075JH04
TTTTTTTATI'CACCTTCTCCG GGGAGGACGAUGCGGUUU U JUUAUUCACCUUCUCCGCAGACGACUCGCCCGA 5076 . HQ4
TT G CTCCTTCTTATTTCCG GGGAGGACGAUGCGGUUUGCUCCUUCUUAUUUCCGCAGACGACUCGCCCGA 5077_H04
GCTGTTATTTTTGTTATCCG GGGAGGACGAUGCGGGCUGU UAUU UUUGUUAUCCGCAGACGACUCGCCCGA 5078_H04
ACTGATTTCTCTTTTACCCC GGGAGGACGAUGCGGACUGAUUUCUCUUSJUACCCCCAGACGACUCGCCCGA 5079_H04
CTCTTACC ATG CTTTCTCCG GGGAGGACGAUGCGGCUCUUACCAU6CUUUCUCCGCAGACGACUCGCCCGA 5080_H04
GATTTGTTTTTTGTTTTGTG C GGGAGGACGAUGCGGGAUU UGU UUUUUGUUUUGUGCCAGACGACUCGCCCGA 5081_H04
TTTTATCTTTTG i I 1 1 I I GCG GGGAGGACGAUGCGGUUUUAUCUUUUGUUU UUUGCGCAGACGACUCGCCCGA 5082_H04
TTTGTTTTTCTCTTllTTTCCG GGGAGGACGAUGCGGUUUGUUUUUCUCUUUUUUUCCGCAGACGACUCGCCCGA S083„H04
TCATCTTATATTCCTTTCCG GGGAGGACGAUGCGGUCAUCUUAUAUUCCUUUCCGCAGACGACUCGCCCGA 5G84„.H04
TATTTTCG AG T ATTTTTCCG GGGA6GACGAUGCGGUAUUUUCGAGUAUUUUUCC6CAGACGACUCGCCCGA 5085„H04
GTTTCCCTTGTTTG CTTCCG GGGAGGACGAUGCGGGU UUCCCUUG UUUGCUUCCGCAGACGACUCGCCCGA 5087_H04
AGAGGAGGAGTTGGGATCTG GGGAGGACGAUGCGGAGAGGAGGAGUUGGGAUCUGCAGACGACUCGCCCGA 5088_H04
ΑΑΤΠ ACATTACTCTITGCC GGGAGGACGAUGCGGAAUUUACAUUACUCUUUGCCCAGACGACUCGCCCGA 5089_H04
G CCCTTCTCGTCTTATACTG GGGAGGACGAUGCGGGCCCUUCUCGUCUUAUACUGCAGACGACUCGCCCGA 5090JH04
ATCTTTAT I 1 1 1 1 1 TCTCCG GGGAG6ACGAUGCGGAUCUUUAUUUUUU UUCUCCGCAGACGACUCGCCCGA 5091JH04
ACCCTTTTATTTTCTTG CCG GGGAGGACGAUGCGGACCCUUUUAUUUUCUUGCCGCAGACGACUCGCCCGA 5092_H04
TACTTTTTTTTTCTCTCCG GGGAGGACGAUGCGGUACUU UUUUUUUCUCUCCGCAGACGACUCGCCCGA 5093 H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc CCTTTTACTTTTCTTCTCCG GGGAGGACGAUGCGGCCUU UUACUUUUCUUCUCCGCAGACGACUCGCCCGA 5094. _H04
CTTTACTTCTTTCCTTTCCC GGGAGGACGAUGCGGCUU UAGUUCUUUCCUU UCCCCAGACGACUCGCCCGA 5095_ .H04
ACCGCTGTTTATTTTTTTCCG GGGAGGACGAUGCGGACCGCUGUUUAUUUU LJU UCCGCAGACGACUCGCCCGA 5096. .H04
TCCCATTTCATTTCTTG CCC GGGAGGACGAUGCGGUCCCAUUUCAUUUCUUGCCCCAGACGACUCGCCCGA 5097_ .H04
ATTCGCTTACTATTTACCG GGGAGGACGAUGCGGAUUCGCUUACUAUUUACCGCAGACGACUCGCCCGA 5099. H04
CCTTATTTllTGTTTCCCAC GGGAGGACGAUGCGGCCUUAUUUUUUGUUUCCCACCAGACGACUCGCCCGA 5101 _H04
ATTAmGTTTCACCTTCAe GGGAGGACGAUGCGGAUUAUUUG UUUCACCUUCACCAGACGACUCGCCCGA 5103. „H04
GCCTTTCGTTTCGCCCTCCC GGGAGGACGAUGCGGGCCUUUCGU UUCGCCCUCCCCAGACGACUCGCCCGA 5104. _H04
GTCCTGTCGTCTGTCTGTCCC GGGAGGACGAUGCGGGUCCUG UCGUCUG UCUGUCCCCAGACGACUCGCCCGA 5105. .H04
G CCTTTCGCTCTTCCCTCCC GGGAGGACGAUGCGGGCCUUUCGCUCUUCCCUCCCCAGACGACUCGCCCGA 5107. ,H04
CACTCTCTTCATTCACTCCG GGGAGGACGAUGCGGCACUCUCUUCAU UCACUCCGCAGACGACUCGCCCGA 5108. .H04
GCCTTTCGTTCTGCCCCTCC GGGAGGACGAUGCGGGCCUUUCGUUCUGCCCCUCCCAGACGACUCGCCGGA 5109. _H04
© GTCTTCTGGTTTATCGTCCC GGGAGGACGAUGCGGGUCUUCUGGUUUAUCGUCCCCAGACGACUCGCCCGA 5111. _H04
CTTATTTTCACTTG CTACCC GGGAGGACGAUGCGGCUUAUUUUCACUUGCUACCCCAGACGACUCGCCCGA 5112. _H04
TTTTTCACTTTTTTCGCCCCC GGGAGGACGAUGCGGU UUUUCACUUUUUUCGCCCCCCAGACGACUCGCCCGA 5113. _H04
TCTTTTTATTCTATCGCCTG GGGAGGACGAUGCGGUCU UUUUAUUCUAUCCCCUGCAGACGACUCGCCCGA 5114. _H04
TTTATTGTTTTCTTCGTGCC GGGAGGACGAUGCGGU UUAUUGUUUUCUUCGUGCCCAGACGACUCGCCCGA 5115. _H04
TCTTGTTTGTTCTTTCTCCG GGGAGGACGAUGCGGUCU UGU UUGUUCUUUCUCCGCAGACGACUCGCCCGA 5116. .H04
CCTTTCTTCG CTTTTTACCG GGGAGGACGAUGCGGCCUUUCUUCGCUUUUUACCGCAGACGACUCGCCCGA 5117. H04
ATATTCTAGTTTT CTTTG CC GGGAGGACGAUGCGGAUAU UCUAGUUUUCUUUGCCCAGACGACUCGCCCGA 5118. _H04
TTTTTG TTATTTTTTG CTCTG GGGAGGACGAUGCGGUUUUUGUUAUUUUUUGCJCUGCAGACGACUCGCCCGA 5119. .H04
ATCCTTGCTATTTCTCTCTG GGGAGGACGAUGCGGAUCCUUGCUAUUUCUCUCUGCAGACGACUCGCCCGA 5120. .H04
CCTATTCCTTTTCACCTCCG GGGAGGACGAUGCGGCCUAUUCCUU UUCACCUCCGCAGACGACUCGCCCGA 5121. .H04
TCTTTATTTCTCTTGTTCG C GGGAGGACGAUGCGGUCUU UAUUUCUCUUGUUCGCCAGACGACUCGCCCGA 5122. _H04
AATACTCTT7 ΤΓΉΊ CCACCG GGGAGGACGAUGCGGAAUACUCU UUUUU UUCCACCGCAGACGACUCGCCCGA 5123. _H04
TTTTTTATTCTCTCGCTGCG GGGAGGACGAUGCGGUUU UUUAU UCUCUCGCUGCGCAGACGACUCGCCCGA 5124. .H04
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc ACCTTTCGTTCTGCCTCCC GGGAGGACGAUGCGGACCU U UCGUUCUGCCUCCCCAGACGACUCGCCCGA 5125_HG4
ATTCTTTTTTGT'T TTTTGCC GGGAGGACGAUGCGGAUUCUUUUUUGU UU UUUUGCCCAGACGACUCGCCCGA S126_H04
TTTG GAGGGTTATGGGTCCG GGGAGGACGAUGCGGU UUGGAGGGUUAUGGGUCCGCAGACGACUCGCCCGA 5127_HG4
TATCGTATTTCCCTTGTCGC GGGAGGACGAUGCGGUAUCGUAUU UCCC.U UGUCGCCAGACGACUCGCCCGA 5128_H04
TTTGAAGGGGCATGGGTCCG GGGAGGACGAUGCGGU UUGAAGGGGCAUGG6UCCGCAGACGACUCGCCCGA 5129J-I04
TTATAGGAGTGGTGGTGGTG GGGAGGACGAUGCGGU UAUAGGAGUGGUGGUGGUGCAGACGACUCGCCCGA 5130_H04
TACTTCTTTTTTCTCTCCG GGGAGGACGAUGCGGUACUUCUUUUUUCUCUCCGCAGACGACUCGCCCGA 5132_ HG4
TTTGAAGGGTGATTGCTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUUGCUCCGCAGACGACUCGCCCGA 5133_H04
TTTGATGGGTGATGGCTCCG GGGAGGACGAUGCGGU UUGAUGGGUGAUGGCUCCGCAGACGACUCGCCCGA 5134_H04
ATTGCAGG GTGATG GGTCCG GGGAGG ACGAUGCGGAUUGCAGGG UGAUGGG UCCGCAGACGACUCGCCCGA 5135__H04
TCAC7TA.CTTTTTTCCTCCG GGGAGGACGAUGCGGUCACU UACUU UUUUCCUCCGCAGACGACUCGCCCGA 5136_H04
AT7TCCTCTTTTTTCTG CCG GGGAGGACGAUGCGGAUUUCCUCUUUUUUCUGCCGCAGACGACUCGCCCGA 5138_ H04
TATTAGGAGTAGGTAGGGTG GG6AGGACGAUGCGGUAUUAGGAG UA6GUAGGGUGCAGACGACUCGCCCGA 5139_ H04
TCTTTTTATTTGCTTTCCG GGGAGGACGAUGCGGUCUUUU UAUUUGCUUUCCGCAGACGACUCGCCCGA 5140_H04
CCTTTTACTTCTTTTCTCCG GGGAGGACGAUGCGGCCUUUUACUUCUUUUCUCCGCAGACGACUCGCCCGA 5141„H04
TCTTTGTCTTGTTTTFTCCG GGGAGGACGAUGCGGUCUUUG UCU UGUUUUUUCCGCAGACGACUCGCCCGA 5142_H04
TTTTACATTTACTTTTTCGC GGGAGGACGAUGCGGUUUUACAUUUACUUUUUCGCCAGACGACUCGCCCGA 5143_ H04
GTTCTTACTTTCTTTTCGCC GGGAGGACGAUGCGGGUUCUUACUUUCUUUUCGCCCAGACGACUCGCCCGA 5144_H04
CTTTTTTG CTCTTTTTCTCCG GGGAGGACGAUGCGGCUUUUU UGCUCUU UU UCUCCGCAGACGACUCGCCC6A 5145_H04
ATCTACATTACTTCTTACCC GGGAGGACGAUGCGGAUCUACAUUACUUCUUACCCCAGACGACUCGCCCGA 5146_H04
GTTGTTTTATTTTTTGTCCC GGGAGGACGAUGCGGGUUGUU UUAUUUUUUG UCCCCAGACGACUCGCCCGA 514?_H04
ATTCCAC! 1 1 1 ! S I ! I CCACCG GGGAGG ACGAUGCGGAU UCCACU UU U UUU U UCCACCGCAG ACGACUCGCCCGA 5148_H04
TTT ATATTTTGTG CTTCTC GGGAGGACGAUGCGGUUUAUAUUUUGUGCUUCUCCAGACGACUCGCCCGA 5149_H04
TTTCTCTTTTTCTTTCCG GGGAGGACGAUGCGGUUUCUCU UUUUCU UUCCGCAGACGACUCGCCCGA 5150_H04
TTTTAAGGGTGCTGGGTCCG GGGAGG ACGAUGCGGU UU UAAGGGtjGCUGGGUCCGCAGACCACUCGCCCGA 5151_H04
TTATGTTTCTCCCGTGGCCG G GGAGG ACGA UGCGGUUAUGU UUCU CCCG U G G CCG CAG ACQ AC UCG CCCG A 5153 H04
# in K4
HTS read variable region RNA sequences 3 selection selection Selected sequenc AGTTTTTTG rCTTTTTTTCCCC GGGAGGACGAUGCGGAGUUUUUUGUCUUUUUUUCCCCCAGACGACUCGCCCGA 5154 H04
TTTCCATTTCATTATTTCCC GGGAGGACGAUGCGGUUUCCAUUUCAUUAUUUCCCCAGACGACUCGCCCGA 5155 H04
- TTTACTTTACATTTTGCCCA GGGAGGACGAUGCGGUUUACUUUACAUUUUGCCCACAGACGACUCGCCCGA 5156_H04
TTTGTTTTTA I 1 1 I I I GTCCC GGGAGGACGAUGCGGUUUGUUUUUAUUUUUUGUCCCCAGACGACUCGCCCGA 5157_H04
TTTGAAGGGCG ATGG GTTCG GGGAGGACGAUGCGGUUUGAAGGGCGAUGGGU UCGCAGACGACUCGCCCGA 5158„H04
TCTATTTGGTACT I M i l l CCG GGGAGGACGAUGCGGUCUAUU UGGUACUUUUU UUCCGCAGACGACUCGCCCGA 5159_H04
TTCGTCACTGCTCCTGTCCG GGGAGGACGAUGCGGUUCG UCACUGCUCCUGUCCGCAGACGACUCGCCCGA 5160mH04
TCTTTACTTTCTCTTTGCCC GGGAGGACGAUGCGGUCUU UACUUUCUCU UUGCCCCAGACGACUCGCCCGA 5161„H04
TI GATCAATTCTCCCTGCC GGGAGGACGAUGCGGU UUGAUCAAUUCUCCCUGCCCAGACGACUCGCCCGA 5162_H04
TTTGAGGGTTGATGGGTCCG GGGAGGACGAUGCGGU UUGAGGG UUGAUGGGUCCGCAGACGACUCGCCCGA 5163_H04
TCCTGTCGTC GCTC6GCCC GGGAGGACGAUGCGGUCCUGUCGUCUGCUCGGCCCCAGACGACUCGCCCGA 5164_HQ4
TTTGATTCTTTTTGTTTCCG GGGAGGACGAUGCGGU UUGAUUCUUUUUGUUUCCGCAGACGACUCGCCCGA 5165_H04
TTCTGTCGTC7TTGCGTCCC GGGAGGACGAUGCGGUUCUGUCGUCUUUGCGUCCCCAGACGACUCGCCCGA 5166_ H04
AAACTAGTAGTACTGGT6CC GGGAGGACGAUGCGGAAACUAGUAG UACUGGUGCCCAGACGACUCGCCCGA 5167_ HQ4
CATAGGAGTAGGGGGGTAGC GGGAGGACGAUGCGGCAUAGGAGUAGGGGGGUAGCCAGACGACUCGCCCGA 5168_H04
ATTATCTTTTCTTGTTCCG GGGAGGACGAUGCGGAUUAUCUUUUCUUGUUCCGCAGACGACUCGCCCGA 5169_B04
TTTGCGAGTAGGGTGTTCCG GGGAGGACGAUGCGGUUUGCGAGUAGGGUGUUCCGCAGACGACUCGCCCGA 5170_H04
GCCTTTCGTTCTACCCTCC G6GAGGACGAUGCGGGCCUU UCGUUCUACCCUCCCAGACGACUCGCCCGA 5171_H04
GTTTATTCTTTTCCG CTCCG GGGAGGACGAUGCGGG UUUAU UCUUUUCCGCUCCGCAGACGACUCGCCCGA 5172_H04
AAGCCAGGAGATGTTGTGGC GGGAGGACGAUGCGGAAGCCAGGAGAUGUUGUGGCCAGACGACUCGCCCGA 5173_H04
CTTAATCTACTTCCTCTCCG GGGAGGACGAUGCGGCU UAAUCUACUUCCUCUCCGCAGACGACUCGCCCGA 5175 _H04
CGATTCCTCCGGTTC6TCCC GGGAGGACGAUGCGGCGAU UCCUCCGGUUCGUCCCCAGACGACUCGCCCGA 5176„HQ4
GTGCTCGTCTGTTTTCGTCCC GGGAGGACGAUGCGGGUGCUCGUCUGUUUUCGUCCCCAGACGACUCGCCCGA 5177...H04
ACATACTTAATGTGTGTCCC GGGAGGACGAUGCGGACAUACUUAAUGUGUGUCCCCAGACGACUCGCCCGA 5178 H04
TTCTG GCTTG TCGTTG TC CG GGGAGGACGAUGCGGUUCUGGCUUGUCGUUG UCCGCAGACGACUCGCCCGA 5179 H04
AACGTGTTATTTACGTGCCC GGGAGGACGAUGCGGAACGUGUUAUUUACGUGCCCCAGACGACUCGCCCGA 518Q H04
—
Figure imgf000324_0001
c
O m X
c
Figure imgf000324_0002
# in H4
HTS read variable region RNA sequences # in H3 selection selection Selected sequenc GTTATTTC ! I I Ί I Ί GCTG GGGAGGACGAUGCGGGUUAU UUCUUUUUUGCUGCAGACGACUCGCCCGA 5208_H04
TTCAATCATTTCTTCTCGTG GGGA6GACGAUGCGGUUCAAUCAUU UCU UCUCGUGCAGACGACUCGCCCGA 5209„H04
AGTTCCTTTTCTACTTTCCG GGGAGGACGAUGCGGAGUUCCUUUUCUACUU UCCGCAGACGACUCGCCCGA 5210_H04
TCACTTAGTCTTTTTCTCTG GGGAGGACGAUGCGGUCACUUAGUCUUUUUCUCUGCAGACGACUCGCCCGA 5211_H04
TAT7 CACTCATTTTACTCCG GGGAGGACGAUGCGGUAUUCACUCAUUUUACUCCGCAGACGACUCGCCCGA 5212_H04
CTCT FTTG CTTCTTTTG CC GGGAGGACGAUGCGGCUCUUU UUGCUUCUU UUGCCCAGACGACUCGCCCGA 5213_H04
ATTTCTCTTCCTTTTTACCG GGGAGGACGAUGCGGAUU UCUCUUCCUUUUUACCGCAGACGACUCGCCCGA 5214_H04
CCATTTTCTTTCATCTTCCG GGGAGGACGAUGCGGCCAUUUUCUUUCAUCUUCCGCAGACGACUCGCCCGA 5215_H04
G ATTCTTCACTTTTTACCTG GGGAGGACGAUGCGGGAUUCUUCACU UUUUACCUGCAGACGACUCGCCCGA 5216JH04
CAATTG i ! I I I ITTCACCGC GGGAGGACGAUGCGGCAAUUGUUUUUUUUCACCGCCAGACGACUCGCCCGA 5217_ H04
TTTGCTTTA'i' t I i 'l ! GTCCC GGGAGGACGAUGCGGUUUGCU UUAUUUUUUGUCCCCAGACGACUCGCCCGA 5218_H04
ACTTATCTTTCTCACTACCC GGGAGGACGAUGCGGACUUAUCUUUCUCACUACCCCAGACGACUCGCCCGA 5219_H04
CTTTTTATC AG TTTCTCC G C GGGAGGACGAUGCGGCUUU UUAUCAGUUUCUCCGCCAGACGACUCGCCCGA S22Q_H04
TTAATTTATTTTCAGCTCCC GGGAGGACGAUGCGGUUAAUUUAUUUUCAGCUCCCCAGACGACUCGCCCGA 5221. H04
CATTTACATTCTTTCTGCGC GGGAGGACGAUGCGGCAUU UACAUUCU UUCUGCGCCAGACGACUCGCCCGA 5222_H04
ATTTTTTTTCI Π I I I'G CCG GGGAGGACGAUGCGGAUUUUU UUUCUU UUU UGCCGCAGACGACUCGCCCGA 5223„H04
TTTTTTCACCTTTTGCTTCCG G6GAGGACGAUGCGGU UUUUUCACCU UUUGCUUCCGCAGACGACUCGCCCGA 5224_ HQ4
ACTTTCTCTTATCTCTGCTG GGGAGGAGGAUGCGGACUUUCUCUUAUCUCUGCUGCAGACGACUCGCCCGA 5225JH04
TTTTCCAGTTTG CTTTTCCC GGGAGGACGAUGCGGU UUUCCAGUUUGCUUUUCCCCAGACGACUCGGCCGA 5226JH04
TCTATTTC G AATTCTCTCCG GGGAGGACGAUGCGGUCUAU UUCGAAU UCUCUCCGCAGACGACUCGCCCGA 5227_H04
CATTTTCTCCATTTTCGGCC GGGAGGACGAUGCGGCAUUUUCUCCAUUUUCGGCCCAGACGACUCGCCCGA 522S_H04
TTCATTTTCCCGTTTTGCCG GGGAGGACGAUGCGGUUCAUUUUCCCGUU UUGCCGCAGACGACUCGCCCGA 5229_M04
TCTTTTTCG l i i l' Π TACCG GGGAGGACGAUGCGGUCUU UUUCGUUUUUU UACCGCAGACGACUCGCCCGA 5230_H04
GCCCTTCGTTCTGCCTCCC GGGAGGACGAUGCGGGCCCUUCGDUCUGCCUCCCCAGACGACUCGCCCGA 5231_H04
CCTTACTTTTTTCACCACCC GGGAGGACGAUGCGGCCUUACUUUUUUCACCACCCCAGACGACUCGCCCGA 5232 JH04
CATTTGTCCTGTTTTCACCG GGGAGGACGAUGCGGCAUUUGUCCUGUUU UCACCGCAGACGACUCGCCCGA 5233 H04
# in H4
HTS read variable region RNA sequences selection selection Selected sequenc TATTTATTT7TCTT6CCCCG GGGAGGACGAUGCGGUAU UUAUU UUUCUUGCCCCGCAGACGACUCGCCCGA 5234_HQ4
ATTTTTGTTACG TTG CTC CG GGGAGGACGAUGCGGAUUU U UG U UACG U UG CUCCGCAG ACGACUCGCCCGA 5235_H04
TTfACTTTCTTCTTTTGCCG GGGAGGACGAUGCGGUUUACUUUCUUCUUUUGCCGCAGACGACUCGCCCGA 5236JH04
TTTTTTGTT ATTTTTG CTCTG GGGAGGACGAUGCGGUUUUUUGUUAUUUUUGCUCUGCAGACGACUCGCCCGA 5237_H04
TTTTACTTCCTCA7TTGCCG GGGAGGACGAUGCGG U UUUACUUCCUCAU UUGCCGCAGACGACUCGCCCGA 5238_H04
TTTTTTTT CCTATCTCTGTC CG GGGAGGACGAUGCGGUUUUU UUUCCUAUCUCUGUCCGCAGACGACUCGCCCGA 5239 _HD4
TTG CCTTTTATTTTTTTTTCCG GGGAG6ACGAUGCGGUUGCCUUUUAUUUUUUUUUCCGCAGACGACUCGCCCGA 5240_H04
TTTTTCTACTCTTTTTCCG GGGAGGACGAUGCGGUUUUUCUACUCUUUU UCCGCAGACGACUCGCCCGA 5241_ H04
A1TCTCTC ( i l l I ! CTGCCG GGGAGGACGAUGCGGAUUCUCUCUUUUUUCUGCCGCAGACGACUCGCCCGA 5242_H04
CCTTTTCTTCATTTTTTCCG GGGAGGACGAUGCGGCCUUUUCUUCAUUUUU UCCGCAGACGACUCGCCCGA 5243_H04
GCCTTTCGCTCTGCCTCCC GGGAGGACGAUGCGGGCCUUUCGCUCUGCCUCCCCAGACGACUCGCCCGA 5245_H04
TTTGGGGTTTCCCCGTGCCG GGGAGGACGAUGCGGU UUGGGGU UUCCCCGUGCCGCAG ACGACUCGCCCGA 52 6_ HG4
GCCGTTTCGTTCTGCCCTCCC GGGAGGACGAUGCGGGCCGUUUCGUUCUGCCCUCCCCAGACGACUCGCCCGA 5248_H04
TTCCTGTTTCCTCTG CTCCC GGGAGGACGAUGCGGUUCCUGUUUCCUCUGCUCCCCAGACGACUCGCCCGA 5251_HG4
GTCTTCTGG C1TATTGTCCC GGGAGGACGAUGCGGGUCUUCUGGCUUAUUGUCCCCAGACGACUCGCCCGA 5252_HQ4
ACAGATGGATAGTTGGGGTC GGGAGG ACQ AUGCGGACAGAUGGAUAGUUGGGGUCCAG ACGACUCGCCCGA 5253_H04
CTG AC ATTATCCTG CATGTC GGGAGGACGAUGCGGCUGACAUUAUCCUGCAUG UCCAGACGACUCGCCCGA 5254_H04
TTTGGGAGTAGGGTGATCCG GGGAGGACGAUGCGGUUUGGGAGUAGGGUGAUCCGCAGACGACUCGCCCGA S25S_H04
ACTTTATTCTC I I I Π I I t CCG GGGAGGACGAUGCGGACUUUAU UCUCUUUUU UUUCCGCAGACGACUCGCCCGA 5256JH04
TTTGAAGGGTGATTGTTCCG GGGAGGACGAUGCGGUUUGAAGGGUGAUUGUUCCGCAG ACGACUCGCCCGA 5257_H04
ATTTCTCTTCTTCTTTTGCCG GGGAGGACGAUGCGGAUUUCUCUUCUUCUUU UGCCGCAGACGA.CUCGCCCGA 5259JH04
CTTG AAG G GTG A AG G GTC CG GGGAGGACGAUGCGGCUUGAAGGGUGAAGGGUCCGCAG ACGACUCGCCCGA 5260_H04
ATTCTCTTCTTCTTTTACCG GG6AG6ACGAUGCGGAU UCUCU UCUUCUU UUACCGCAGACGACUC6CCCGA 5262„H04
TTTGATG6GTGAC6GGTCCG GGGAGG ACGAUGCGGUUUGAUG6GUGACGGGUCCGCAGACGACUCGCCCGA 5263...HG4
TCTTCnTnTTCACTTTCCG GGGAGGACGAUGCGGUCUUCUUUUUUUCACUUUCCGCAGACGACUCGCCCGA 5264_H04
TTTATTTTCTTGTTG TTGTG GGGAGGACGAUGCGGU UUAUU UUCUUGUUGUUGUGCAGACGACUCGCCCGA 5265J-I04
Although the foregoing specification and examples fully disclose and enable the present invention, they are not intended to limit the scope of the invention, which is defined by the claims appended hereto.
All publications, patents and patent applications are incorporated herein by reference. While in the foregoing specification this invention has been described in relation to certain embodiments thereof, and many details have been set forth for purposes of illustration, it will be apparent to those skilled in the art that the invention is susceptible to additional embodiments and that certain of the details described herein may be varied considerably without departing from the basic principles of the invention.
The use of the terms "a"' and "an" and "the" and similar referents in the context of describing the invention are to be construed to co er both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms "comprising," "having," 'including," and "containing" are to be construed as open-ended terms (i. e. , meaning "including, but not limited to") unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein, or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. , "such as"') provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating an non-claimed element as essential to the practice of the invention.
Embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. Variations of those embodiments may become apparent to those of ordinary skill in the art upon reading the foregoing description. The inventors expect skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is
encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.

Claims

WHAT IS CLAIMED IS: ί . A nucleic acid molecule not more than 90 nucleotides in length comprising an
aptamer, wherein the aptamer specifical ly targets extracellular histones,
2. The nucleic acid molecule of claim 1 , wherein the aptamer specifically targets 1-13 and/or H4.
3. The nucleic acid molecule of claim 2, wherein the aptamer comprises at least 90% identit to an RNA sequence listed in Table 1 .
4. The nucleic acid molecule of claim 2, wherein the aptamer comprises at least. 95% identity to an RNA sequence listed in Table 1.
5. The nucleic, acid molecule of claim 2, wherein the aptamer comprises 1.00% identity to an RNA sequence listed in Table 1 .
6. The nucleic acid molecule of any one of claims 1-5, wherein the aptarner specifically targets human H3 and H4.
7. The nucieic acid molecule of claim 6, wherein the aptanier comprises at least 90% identity to KU L KU2 or KU3.
8. The nucleic acid molecule of claim 6. wherein the aptamer comprises at least 95% identity to KUL U2 or K.U3,
9. Fhe nucleic acid molecule of claim 6, wherein the aptamer has 100% identity to KU1 , U2 or U3.
10. The nucleic acid molecule of any one of claims 1 -5. wherein the aptamer specifically targets human 113.
1 1. The nucleic acid molecule of claim 10. wherein the aptamer comprises at least 90% identity to K.U7, KU8 or KU9.
12. The nucleic acid molecule of claim 10, wherein the aptamer comprises at least 95% identity to KU7, U8 or KU9.
13. The nucleic acid molecule of ciaim 10, wherein the aptamer has 100% identity to KD7, KU8 or KU9.
1 . The nucleic acid molecule of any one of claims 1 -5, wherein the apiamer specifically targets human 14.
15. The nucleic acid molecule of claim 14, wherein the aptamer comprises at least 90% identity to KU4, KU5 or U6.
16. The nucleic acid molecule of claim 14. wherein the aptamer comprises at least 95% identity to U4, U5 or KU6.
17. The nucleic acid molecule of claim 14. wherein the aptamer has 1 0% identity to KU4. KU5 or U6.
1 8. The nucleic acid molecule of any one of claims 1 to 17, wherein the nucleotides are R A.
19. The nucleic acid molecule of claim 18, wherein the RNA comprises a modified nucleotide.
20. 1 he nucleic acid molecule of claim 19, wherein the chemically modified RNA
comprises a 2' substituted sugar such as 2'-0-methyl-; 2'-0-(2-methoxyethyl); 2-0- al!yi; 2-O-allyl; 2'-S-alkyl; 2'-S-al3yl; 2'-iluoro-; 2'-amino 2'-halo or 2-azido-ribose, a carbocyclic sugar analogue, an a-anomeric sugar; an epimeric sugar such as arabinose, xylose or lyxose, a pyranose sugar, a furanose sugar, sedoheptulose; 2'- fluoro-p-D-arabinonucleotide (FANA) modificatioiv, or a Locked nucleic acid (LNA).
The nucleic acid molecule of claim.20. wherein 2'O e modi fied bases are alternated with Uft-modified RNA bases.
The nucleic acid molecule of claim 1 , wherein the chemically modified RNA comprises a 2'-iluoro pyrimidine and/or a 2' O-meth.y.1 pyrimidine.
The nucleic acid molecule of claim 19, comprising an 2'OMe purine and a 2'-fluoro pyrimidine.
The nucleic acid molecule of claim 19, wherein the nucleic acid comprises a bridging ph.osphorothioaie, and end cap that reverses the polarity of the chain and/or a linker.
A conjugate comprisi ng the nucleic acid molecule of any one of claims 1-24 linked to a therapeutic molecule.
The conjugate of claim 25, wherein the therapeutic molecule is an RNAi molecule.
The conjugate of claim 26, wherein the RNAi molecule is an siRNA molecule or an miRN.A molecule.
A pharmaceutical composition comprising a pharmaceutically acceptable carrier and (i ) the nucleic acid molecule of any one of claims 1 to 24; or
(ii) the conj ugate of any one of claims 25 to 27.
A method for treating a subject having or being disposed to having a bistone-mduced injury or disease comprising administering the composition as described in claim 28 to the subject.
30. :Fhe method of claim 29. wherein the subject is a mammal. 1 . The method of claim 30, wherein the subject is a human.
32. The method of any one of claims 29 to 31 , wherein the administration is by
intravenous injection or by inhalation.
33. The method of any one of claims 29 to 32, wherein the composition is administered within 0-24 hours after an injury or diagnosis of disease.
34. The method of any one of claims 29 to 32, wherein the composition is administered within 0-12 hours after an injury or diagnosis of disease.
35. The method of claim 34, wherein the injury is trauma, bum, sepsis, iransfusi on-related acute lung injury, organ ischemia or infarction, or inhalation lung injury.
36. The method of any one of claims 29 to 32. wherein the composition is administered in response to a prolonged severe disease,
37. The method of any one of claims 29 to 36, wherein the composition is administered more than one time.
38. The method of claim 37. wherein the composition is administered two, three, four, five or six times.
39. The method of any one of claims 29 to 38, wherein the disease is an autoimmune disease (such as systemic lupus erythematosus), arthritis, rheumatoid arthritis, or juvenile arthritis; edema: sepsis; septic shock; inflammation: non-septic hyper inflammatory disorder; infectious disease; thrombosis: nephritis; inflammatory liver injury: traumatic hemorrhage: acute pancreatitis; acute respiratory distress syndrome: ischemic injury; ischemia-reperfusioii injury; ischemic stroke; cardiovascular disease; atherosclerosis; myocardial infarction; radiotherapy toxicity; cytokine therapy toxicity; granulomatous disease; asthma; graft-vs. host disease; cachexia; a coagulopathy; inhalation injury; trauma; cancer; burn effects; multiple organ dysfunction syndrome (MODS i/aenle respiratory distress syndrome (ARDS); and/or complications thereof
40. The method of any one of claims 29 to 39, further comprising administering at least one other therapeutic agent to the subject, before, concurrentl with or after the composition.
41 . The method of claim 40, wherein the therapeutic agent is selected front the group consisting, of an antibody, an antibody fragment, an immune conjugate, a
radionuclide, an immunomodulatory, an anti-angiogeuic agent, a pro-apoptotic agent, a cytokine, a chemokine, a drug, a toxin, a hormone, an siRNA, a cytokine, a chemokine, a coagulation inhibitor, a stern cell growth factor, a lymphotoxin, a hematopoietic factor, a colony stimulating factor, an interferon, erythropoietin, thrombopoietin, an enzyme, recombinant human ihrombomoduiin and activated human protein ,
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