WO2015198345A1 - An apparatus for separating blood components - Google Patents

An apparatus for separating blood components Download PDF

Info

Publication number
WO2015198345A1
WO2015198345A1 PCT/IN2015/000259 IN2015000259W WO2015198345A1 WO 2015198345 A1 WO2015198345 A1 WO 2015198345A1 IN 2015000259 W IN2015000259 W IN 2015000259W WO 2015198345 A1 WO2015198345 A1 WO 2015198345A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
pressure plate
apparatus
pressure
blood
bag
Prior art date
Application number
PCT/IN2015/000259
Other languages
French (fr)
Inventor
Omprakash BENIWAL
Shailendra Mohan MATHUR
Original Assignee
Beniwal Omprakash
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61MDEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02Blood transfusion apparatus
    • A61M1/029Separating blood components present in distinct layers in a container, not otherwise provided for

Abstract

Semi automated Blood component Extractor of the present invention is a mechanical device for separating plasma and buffy coat removed RBCs. It exerts uniform pressure on the blood bags during the separation of blood components. The purpose of this innovation is to generate Leucocytes Reduced Red Blood Cells at blood banks to reduce the adverse blood transfusion reactions.

Description

AN APPARATUS FOR SEPARATING BLOOD CO PO E S

TECHNICAL FIELD OF THE INVE TION

The pf<2$Sfrt jt«ve«t:fc« . relates medical devices. aiticui ri) t e present invention relates to devices for separation of blood components especially, segre at on of R BCs fmm lasms a d h» ny COEH m blood.

BACKGROUND ART

Blood consists of var ious components. These components e s be ..broacHv divided into the fo l!owmg ivvo parts;

♦ · Solid particles- The solid particles include Red Blood Cells RB.Cs), White Blood Celis (WBC Le«cocytes) and Platelets. The solid particles are segregated by centrifuge ion such that red blood cells settle at the bottom While white feiood cells and platelets settle in a separate layer above RBCs, This layer of white blood cells and platelets that settle above RBCs after eentrifugstion is: called as "buffy coat';

* Plasma- The remaining part of the blood constitutes plasma. :\t is more than

9 % water. The Plasm also contains hormones, proteins etc. as it acts a a solvent lor them.

The separation, of plasma, white cells, red cells, platelets, etc; from a whole blood solution provides a variety of uses which are well known throughout the field of medicine. In 'India, many blood banks have the facility of sepa atin blood components but they are not capable separating Leucocytes Reduced Red Blood Cells. This is because the available automated plasma expressor top and bottom is very costly and most of the blood banks are unable to afford it. Even government hospitals are unable to afford it because of its higher price. At present in market two types of machines are available to separate blood components. One is fully automatic (imported) which is known as automated top and bottom and another is manually operated top and top machine. The automatic machine is very expensive and hospitals are unable to afford it; whereas; manually operated machine which is known as Top Plasma Expressor does not provide Leucocytes Reduced Red Blood cells. The different components of blood are first segregated by centrifugation when different components separate out weight-wise such that heaviest components settle at the bottom. The segregated components are then separated from expressor devices. A uniform pressure is applied on the bag containing segregated components when the. plasma moves to the top due to pressure and RBCs(which are heavier) move to the bottom. On applying a uniform pressure the plasma is taken out from the top while RBCs are removed from the bottom. In order to efficiently separate the components, it is necessary to apply the appropriate pressure uniformly such that RBCs are not mixed in the plasma or burTy coat. The outflow of plasma has to be stopped at exactly before the RBC layer begins to outflow from the top plasma side in order to prevent RBCs from contaminating the plasma or buffy coat. OBJECTS OF THE INVENTION

An object of the invention is to propose a system to avail Leucocytes Reduced RBCs at ail component blood banks to reduce the adverse blood transfusion reactions;

Another object of the present invention is to separate plasma from whole blood;

Another object is to separate platelets concentrate from buffy coat;

Another object of this invention is that it can be used by any blood bank as its cost is affordable and beneficial for patients; and

Another object of this invention is any top and bottom bag and top and top bag can be used on this machine for any kind of separation based on specific gravity principal.

SUMMARY OF THE INVENTION

Semi automated Blood component Extractor of the present invention is a mechanical device for separating plasma and buffy coat removed RBCs. It exerts uniform pressure on the blood bags during the separation of blood components. The purpose of this innovation is to generate Leucocytes Reduced Red Blood Cells at blood banks to reduce the adverse blood transfusion reactions. It will be an alternative to commercially available imported automated plasma, expressor top and bottom, which are very costly. The main components of the machine are linear actuator, pressure lock knob, pressure plates, motors, micro switches, flow control locks etc. BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS

The foregoing and other objects, aspects and advantages of the invention will be better understood from the following drawings of the invention in which:

Fig. 1 A illustrates a front view of plasma expressor device according to an embodiment of the present invention:

Fig I B illustrates a rear view of plasma expressor device according to an embodiment of the present invention; and

Fig.2 illustrates a pressing assembly according to an embodiment of the present invention.

DESCRIPTION OF A PREFERRED EMBODIMENT

In describing the embodiments of the invention, specific terminology is resorted for the sake of clarity. However, it is not intended that the invention be limited to the specific terms so selected and it is to be understood that each specific term includes all technical equivalents that operate in a similar manner to accomplish a similar purpose.

Illustrative embodiments of the invention now will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the invention are shown. Indeed, the invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements.

Fig. I Λ shows a front view of plasma expressor device according to an embodiment of the present invention which comprises a pressurizing section having a first pressure plate (3) and a second pressure plate (2). The second pressure plate (2) is preferably made up of transparent Acrylic plate so that its user is able to monitor a bag of segregated blood components while the components are being separated. The second pressure plate (2) is locked on the frame by a second plate lock (1).

The bag is hung between the first pressure plate (3) and the second pressure plate (2) on a hanger (9) such that the bag can be pressed by the said two pressure plates (3,2). Pressure is applied on the bag by moving the first pressure plate (3) against the second pressure plate (2) while the bag is in between them.

According to an embodiment, the first pressure plate (3) is moved by way of a hydraulic mechanism (Figure I B) configured to move the first pressure plate (3) in a linear manner towards the second pressure plate (2). The hydraulic mechanism (Figure I B) comprises a motor ( 101 ) driven by a battery ( 103) located at the rear of the device. The motor ( 101 ) drives a hydraulic pump (102) that causes the first pressure plate (3) to move linearly towards or against the second pressure plate (2). The hydraulic pressure is controlled by a hydraulic pressure lock ( 104). The motor 101 may also be actuated by connecting it to A.C. mains directly or through an A.C. to D.C. converter. The flow of RBCs and Plasma can be controlled by the respective locks (8, 1 1) manually.

In another embodiment a linear actuator is connected to a motor which causes the movement of a linear actuator and subsequently, that of the first pressure plate (3). In both the cases, the speed and rotation of the motor 101 is variable by a regulator (not shown). The movement and the speed of.moving of the first pressure plate can be controlled accordingly. The speed regulator according to an embodiment is a microcontroller behind the control panel ( 10) for regulating speed of the motor ( 101 ). In yet another embodiment, the hydraulic pump or the linear actuator are controlled manually.

Returning to Figure 1 , the first pressure plate (3) is thick steel coated with silicon and rubber. The linear actuator or the hydraulic pump moves the first pressure plate (3 ) such that a uniform pressure is applied on the bag. The second pressure plate (2) has the second plate lock (1 ) on one side and the other side is fixed with a hinge joint (4), which will allow the bag to be fixed between the first and second plates (3, 2). There is a gap above and below the plate allowing top and bottom satellite bags to be attached. This differs from the conventional manual plasma expressor in which there is a gap only on the top while the lower end of the plate is fixed to the body.

In operation, on the front side the second pressure plate (2), which is transparent and has a second pressure plate lock ( 1 ), is unlocked. The centrifuged bag containing a liquid (preferably blood) is hung on the hanger (9) and the second pressure plate (2) is thereafter locked. The motor starts moving the actuator or hydraulic pump (not shown) which moves the first pressure plate (3) and a uniform pressure is applied on the bag.

When a uniform pressure is applied on the bag, the plasma flows upwards and it is extracted through plasma lock (1 1) where a plasma satellite bag is connected. The plasma lock ( 1 1 ) controls starting and stopping of the flow of plasma into the plasma satellite bag. Similarly, a RBC lock (8) is located at the bottom to control the flow of RBCs into a RBC satellite bag.

After applying the pressure, the plasma lock ( I I ) is opened on the top of the bag for plasma satellite bag connected to the plasma lock ( I I ) by a plasma tube. As mentioned before, flow of plasma in the plasma tube is controlled by the plasma lock ( I I ). The flow of plasma and RBCs from the plasma lock (1 1 ) and the RBC lock (8), respectively, may be manually controlled or may be controlled by motors driven by microcontroller either remotely or automatically. Similarly platelet concentrates can be removed from buffy coat and transferred into a platelet satellite bag.

Fig. 2 illustrates a pressing assembly according to an embodiment of the present invention. The pressing assembly comprises a linear actuator (6) that moves in horizontal direction pushing a first pressure plate (3) towards and away from a second pressure plate (2) locked by a second pressure plate lock ( 1 ) and moveable by a hinge joint (4). A bag on which the pressure is to be applied (containing the blood) is fixed between the two plates (2, 3) and a uniform pressure is applied on it by the first pressure pfate (3) as it presses the bag against the second pressure plate (2). The linear actuator (6) is driven by a motor (5) connected to the linear actuator (6) through a set of gears (not shown) that transform rotary motion to a linear motion. The gears may also be used to control the speed of the linear actuator.

In a preferred embodiment, the speed of motor (5) is controlled by a microcontroller installed in the plasma expressor such that variable speeds of the motor (5) can be achieved. Sometimes it is preferred to exert the pressure on the bag in a slow manner to achieve high efficiency in separation. Experiment:

In an experiment conducted on the preferred embodiment of the present invention using a small motor and a hydraulic system the following results were obtained:

At slow speed, the total time of operation was 4. minutes 50 seconds and leukocytes reduction was found to be 91 percent whereas in combination of slow and fast speed, the time was reduced to 3 minutes. The results are at par with the automated plasma expressor which is very expensive and unaffordable by various blood banks.

The device successfully separated Red cell concentrate, leucocytes reduced RBCs, platelets from PRP, Platelets from buffy coat, Granulocyte from buffy coat, FFP, CRYO and CPP. While the invention has been particularly shown and described with reference to exemplary embodiments thereof, the invention is not limited to these embodiments. It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from' the spirit and scope of the invention as defined by the following claims.

The above disclosure is non-limiting and modifications and variations are possible without departing from the spirit arid scope of the invention. Since other modifications and changes varied to fit particular operating requirements and environments are apparent to those skilled in the art, the invention is not considered limited to the example chosen for purposes of disclosure, and covers all changes and modifications which do not constitute departures from the true spirit and scope of this invention.

Having thus described the invention, what is desired to be protected by Letters Patent is presented in the subsequently appended claims. Although the invention has been explained in relation to its preferred embodiment, it is to be understood that many other possible modifications and variations can be made without departing from the spirit and scope of the invention as herein described.

As one of ordinary skill in the art may appreciate, the example process described herein can be modified. For example, certain steps can be omitted, certain steps can be carried out concurrently, and other steps can be added. Although particular embodiments of the invention have been described in detail, it is understood that the invention is not limited correspondingly in scope, but includes all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto. This written description uses examples to disclose the invention, including the best mode, and also to enable any person skilled in the art to practice the invention, including making and using any devices or systems and performing any incorporated methods. The patentable scope the invention is defined in the claims, and may include other examples that occur to those skilled in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that do not differ from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal languages of the claims.

Claims

We claim:
1. An apparatus for separating blood components comprising:
a first pressure plate moveable in linear direction juxtaposed to a second pressure plate;
means to detachably place a liquid bag between the first and second pressure plates such that movement of the first pressure plate exerts pressure on the blood bag;
means to extract one component from the blood bag from top and means to extract a second component from the bottom;
wherein, the first pressure plate is self actuated.
2. The apparatus as claimed in claim 1 , wherein, the first pressure plate is driven by a linear actuator.
3. The apparatus as claimed in claim 1 , wherein, the first pressure plate is driven by a hydraulic pump.
4. The apparatus as claimed in any of the above claims wherein, the movement of the first pressure plate is controlled by a micro-controller.
5. The apparatus as claimed in any of the claims 1 to 3, wherein the first pressure plate exerts a uniform pressure on the liquid bag by controlling it manually.
6. The apparatus as claimed in any of the claims 1 to 4, wherein the first pressure plate is configured to move at different variable speeds and pressure.
7. The apparatus as claimed in any of the above claims, comprising at least one tube connected to the apparatus to collect at least one component of the liquid.
8. The apparatus as claimed in claim 6, wherein flow in the at least one tube is controlled by a lock.
9. The apparatus as claimed in claim 7, wherein the lock is operated manually.
10. The apparatus as claimed in claim 7, wherein the lock is automatically operated by a microcontroller.
1 1. A method for separating blood components comprising:
arranging of first pressure plate to be moveable in linear direction juxtaposed to a second pressure plate;
detachably placing a liquid bag between the first and second pressure plates such that movement of the first pressure plate exerts pressure on the blood bag;
extracting one component fi om the blood bag from top and means to extract a second component from the bottom.
PCT/IN2015/000259 2014-06-25 2015-06-24 An apparatus for separating blood components WO2015198345A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
IN1127/DEL/2014 2014-06-25
IN1127DE2014 2014-06-25

Publications (1)

Publication Number Publication Date
WO2015198345A1 true true WO2015198345A1 (en) 2015-12-30

Family

ID=54937500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2015/000259 WO2015198345A1 (en) 2014-06-25 2015-06-24 An apparatus for separating blood components

Country Status (1)

Country Link
WO (1) WO2015198345A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060018B2 (en) * 2003-09-11 2006-06-13 Cobe Cardiovascular, Inc. Centrifuge apparatus for processing blood

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060018B2 (en) * 2003-09-11 2006-06-13 Cobe Cardiovascular, Inc. Centrifuge apparatus for processing blood

Similar Documents

Publication Publication Date Title
US3655123A (en) Continuous flow blood separator
US5252228A (en) Cytocentrifugation device, apparatus, and method
US7011761B2 (en) Red blood cell processing systems and methods which control red blood cell hematocrit
US4285464A (en) Apparatus for separation of blood into components thereof
US5690815A (en) Automated system for processing biological fluid
US4596547A (en) Valve apparatus for photoactivation patient treatment system
US6730054B2 (en) Blood collection systems and methods that derive estimated effects upon the donor&#39;s blood volume and hematocrit
US7832566B2 (en) Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US5538405A (en) Peristaltic pulse pumping systems and methods
US3050239A (en) Centrifuge apparatus
US7553413B2 (en) Plasma concentrator device
US6027657A (en) Systems and methods for collecting diluted mononuclear cells
Sin et al. Enrichment using antibody‐coated microfluidic chambers in shear flow: Model mixtures of human lymphocytes
US5632906A (en) Apparatus and method for transferring separated component in liquid from a flexible bag in a centrifugal container
US20060196885A1 (en) Method and apparatus for application of a fluid
US6605223B2 (en) Blood component preparation (BCP) device and method of use thereof
US6488860B2 (en) Device and method for separating blood into blood components
US7179391B2 (en) Apparatus and method for separating and concentrating fluids containing multiple components
US20040104182A1 (en) Methods and apparatuses for blood component separation
US6982038B2 (en) Centrifuge system utilizing disposable components and automated processing of blood to collect platelet rich plasma
US6716187B1 (en) Platelet concentration syringe kit
US4718888A (en) Centrifuge bowl mount
US4187979A (en) Method and system for fractionating a quantity of blood into the components thereof
US20030116512A1 (en) Methods and apparatus for separation of particles
EP0014093A1 (en) An apparatus, a method and a disposable blood pathway for use in separating blood into components thereof

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15811727

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15811727

Country of ref document: EP

Kind code of ref document: A1