WO2015189113A1

WO2015189113A1 - Active compound combinations comprising proquinazid and spiroxamine and optionally prothioconazole

Info

Publication number: WO2015189113A1
Application number: PCT/EP2015/062646
Authority: WO
Inventors: Frank Göhlich; Ulrich Krieg; Peter Dahmen; Andreas Goertz; Gunter Meyer
Original assignee: Bayer Cropscience Aktiengesellschaft
Priority date: 2014-06-11
Filing date: 2015-06-08
Publication date: 2015-12-17
Also published as: ZA201700198B; EP3154352A1; EA201692523A1

Abstract

The invention relates to active compound combinations, in particular within a fungicide composition, which comprises (A) proquinazid and (B) spiroxamine and optionally (C) prothioconazole. Moreover, the invention relates to a method for curatively or preventively controlling the phytopathogenic fungi of plants or crops, to the use of a combination according to the invention for the treatment of seed, to a method for protecting a seed and to the treated seed.

Description

Active Compound Combinations Comprising Proquinazid and Spiroxamine and optionally Prothioconazole

Proquinazid, having the chemical name 6-iodo-2-propoxy-3-propyl-4(3H)-quinazolinone (Compound A) and its manufacturing process starting from known and commercially available compounds is described in WO 94/26722. Proquinazid is commercially available under the trade designation Talius^®.

Spiroxamine, having the chemical name 8-tert-butyl-l,4-dioxaspiro[4.5]decan-2- ylmethyl(ethyl)(propyl)amine 8-(l,l-dimethylethyl)-N-ethyl-N-propyl-l,4-dioxaspiro[4.5]decane- 2-methanamine (Compound B) and its manufacturing process starting from known and commercially available compounds is described in EP-A 0 281 842.

Prothioconazole, having the chemical name 2-[2-(l -chlorocyclopropyl)-3-(2-chlorophenyl)-2- hydroxypropyl]-l,2-dihydro-3H- l,2,4-triazole-3-thione (Compound C) and its manufacturing process starting from known and commercially available compounds is described in WO-A 96/16048.

Active compound combinations comprising spiroxamine and further fungicides are disclosed in EP-A 0 627 163, EP-A 0 833 561, EP-A 0 831 702, and WO-A 01/37666. Active compound combinations comprising prothioconazole and further fungicides are disclosed in WO-A 98/47367, WO-A 03/073850, WO-A 03/073851, and WO-A 03/073852. Active compound combinations comprising proquinazid and further fungicides are disclosed in WO 98/33381. According to "Getreide Magazin 1/2008" page 1, col. 4, line 33 - page 2, col. 1, line 3 and table 1 the recommendation was provided to combine the product Input^® containing prothioconazole and spiroxamine with the product Talius^® containing proquinazid to improve the efficacy of the combination prothioconazole and spiroxamine against powdery mildrew. The proposed combination uses 50 g/ha proquinazid, 375 g/ha spiroxamine and 200 g/ha prothioconazole.

Since the environmental and economic requirements imposed on modern-day fungicides are continually increasing, with regard, for example, to the spectrum of action, as well as improving toxicity, selectivity, application rate, formation of residues, and favorable preparation ability, and since, furthermore, there may be problems, for example, with resistances, a constant task is to develop new fungicide agents which in some areas at least have advantages over their known counterparts.

The invention provides active compound combinations or compositions which in some aspects at least achieve the stated objectives. Surprisingly, the active compound combinations according to the present invention provide a broader spectrum of action and reduced application rates. More surprisingly, the active compound combinations according to the present invention provide a broader spectrum of action and reduced application rates in combination with very good yield effects.

It has additionally been found, surprisingly, that the combinations or compositions according to the invention not only bring about the additive enhancement of the spectrum of action with respect to the phytopathogens to be controlled that was in principle to be expected but achieves a synergistic effect which extends the range of action of the component (A) and of the component (B) and optionally of the component (C) in two ways. The rates of application of the component (A) and of the component (B) and optionally of the component (C) are lowered whilst the action remains equally good.

Additionally, very good yield effects were obtained with the combinations or compositions according to the invention.

However, besides the actual synergistic action with respect to fungicidal activity, the fungicidal combinations according to the invention also have further surprising advantageous properties which can also be described, in a wider sense, as synergistic activity. Examples of such advantageous properties that may be mentioned are: a broadening of the spectrum of fungicidal activity to other phytopathogens, for example to resistant strains; a reduction in the rate of application of the active ingredients; adequate fungicidal control with the aid of the compositions according to the invention, even at a rate of application at which the individual compounds are less effective or totally ineffective; advantageous behavior during formulation or upon application, for example upon grinding, sieving, emulsifying, dissolving or dispensing; increased storage stability; improved stability to light; more advantageous degradability; improved toxicological or ecotoxicological behavior; improved characteristics of the useful plants including: emergence, crop yields, more developed root system, tillering increase, increase in plant height, bigger leaf blade, less dead basal leaves, stronger tillers, greener leaf color, less fertilizers needed, less seeds needed, more productive tillers, earlier flowering, early grain maturity, less plant verse (lodging), increased shoot growth, improved plant vigor, and early germination; or any other advantages familiar to a person skilled in the art. The combination according to the invention can also provide an improved systemicity to the active compounds that are used. Indeed, even if some of the used fungicide compounds do not possess any or a satisfying systemicity, within the composition according to the invention these compounds can exhibit such a property.

Furthermore and preferably, the active compound combination according to the present invention allows excellent powdery mildew control.

In a similar manner, the combination according to the invention can allow an increased persistence of the fungicide efficacy of the active compounds that are employed.

Another advantage of the combination according to the invention relies in that an increased curativity is achievable.

Accordingly, the present invention provides an active compound combination comprising:

(A) proquinazid and

(B) spiroxamine and optionally

(C) prothioconazole wherein the weight ratio of A:B is from 1 : 1 to 1 :8. Preferably, the weight ratio of A:B is from 1 : 1 to 1 :7.

In particular this invention provides an active compound combination comprising (A) proquinazid and (B) spiroxamine.

In particular this invention provides an active compound combination comprising (A) proquinazid and (B) spiroxamine and (C) prothioconazole.

The active compound combinations according to the present invention do not comprise cyflufenamid.

In particular this invention provides an active compound combination consisting of (A) proquinazid and (B) spiroxamine and suitable adjuvants, solvents, carrier, surfactants or extenders. In particular this invention provides an active compound combination consisting of (A) pro- quinazid and (B) spiroxamine and (C) prothioconazole and suitable adjuvants, solvents, carrier, surfactants or extenders.

In the combinations according to the invention the compounds (A) and (B) or the compounds (A) and (C) or the compounds (B) and (C) are present in a synergistically effective weight ratio of A:B orA:C orB:C.

In preferred embodiments of the invention the weight ratio of A:B is from 1:1 to 1 :7, preferably from 1 :2 to 1 :6, more preferably from 1 :3 to 1 :6.

In preferred embodiments of the invention the weight ratio of A:C is from 1 : 1 to 1 :20, preferably from 1:1 to 1:10, more preferably from 1 :2 to 1:6.

In preferred embodiments of the invention the weight ratio of B:C is from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 3:1 to 1:3.

In preferred embodiments of the invention the weight ratio of A:B is from 1:1 to 1:7 and the weight ratio of A:C is from 1:1 to 1:20, preferably the weight ratio of A:B is from 1:2 to 1:6 and the weight ratio of A:C is from 1:1 to 1:10, more preferably the weight ratio of A:B is from 1 :3 to 1 :6 and the weight ratio of A:C is from 1 :2 to 1 :6.

In preferred embodiments of the invention the weight ratio of A:B is from 1:1 to 1:7 and the weight ratio of B:C is from 10:1 to 1:10, preferably the weight ratio of A:B is from 1:2 to 1:6 and the weight ratio of B:C is from 5:1 to 1 :5, more preferably the weight ratio of A:B is from 1 :3 to 1 :6 and the weight ratio of B:C is from 3:1 to 1 :3.

In preferred embodiments of the invention the weight ratio of A:C is from 1:1 to 1:20 and the weight ratio of B:C is from 10:1 to 1:10, preferably the weight ratio of A:C is from 1:1 to 1:10 and the weight ratio of B:C is from 5:1 to 1:5, more preferably the weight ratio of A:C is from 1:2 to 1:6 and the weight ratio of B:C is from 3:1 to 1:3.

In preferred embodiments of the invention the weight ratio of A:B is from 1:1 to 1: 7, the weight ration of A:C is from 1:1 to 1:20 and the weight ratio of B:C is from 10:1 to 1: 10, preferably the weight ratio of A:B is from 1 :2 to 1 :6, the weight ration of A:C is from 1:1 to 1:10 and the weight ratio of B:C is from 5:1 to 1:5, more preferably the weight ratio of A:B is from 1:3 to 1 :6, the weight ration of A:C is from 1:2 to 1:6 and the weight ratio of B:C is from 3:1 to 1:3. Where a compound (A) or a compound (B) or a compound (C) can be present in tautomeric form, such a compound is understood hereinabove and hereinbelow also to include, where applicable, corresponding tautomeric forms, even when these are not specifically mentioned in each case.

Compounds (A) or compounds (B) or compounds (C) having at least one basic centre are capable of forming, for example, acid addition salts, e.g. with strong inorganic acids, such as mineral acids, e.g. perchloric acid, sulfuric acid, nitric acid, nitrous acid, a phosphoric acid, a hydro- halic acid, such as hydrogen fluoride, hydrogen chloride, hydrogen bromide and hydrogen iodide, or acidic salts, such as NaHSC and KHSO4, with strong organic acids, formic acid, carbonic acid and alkanoic acids, such as acetic acid, trifluoroacetic acid, trichloroacetic acid and propionic acid, and also glycolic acid, thiocyanic acid, lactic acid, succinic acid, citric acid, benzoic acid, cinnamic acid, maleic acid, fumaric acid, tartaric acid, sorbic acid oxalic acid, al- kylsulphonic acids (sulphonic acids having straight-chain or branched alkyl radicals of 1 to 20 carbon atoms), arylsulphonic acids or aryldisulphonic acids (aromatic radicals, such as phenyl and naphthyl, which carry one or two sulphonic acid groups), alkylphosphonic acids (phosphonic acids having straight-chain or branched alkyl radicals of 1 to 20 carbon atoms), ar- ylphosphonic acids or aryldiphosphonic acids (aromatic radicals, such as phenyl and naphthyl, which carry one or two phosphonic acid radicals), where the alkyl and aryl radicals may carry further substituents, for example p-toluenesulphonic acid, 1,5-naphthalenedisulphonic acid, salicylic acid, p-aminosalicylic acid, 2-phenoxybenzoic acid, 2-acetoxybenzoic acid, etc.. Compounds (A) or compounds (B) or compounds (C) having at least one acid group are capable of forming, for example, salts with bases, e.g. metal salts, such as alkali metal or alkaline earth metal salts, e.g. sodium, potassium or magnesium salts, or salts with ammonia or an organic amine, such as morpholine, piperidine, pyrrolidine, a mono-, di- or tri-lower alkylamine, e.g. ethyl-, diethyl-, triethyl- or dimethyl-propyl-amine, or a mono-, di- or tri-hydroxy- lower alkylamine, e.g. mono-, di- or tri-ethanolamine. In addition, corresponding internal salts may optionally be formed. In the context of the invention, preference is given to agrochemically advantageous salts. In view of the close relationship between the compounds (A) or the compounds (B) or compounds (C) in free form and in the form of their salts, hereinabove and herein below any reference to the free compounds (A) or free compounds (B) or free compounds (C) or to their salts should be understood as including also the corresponding salts or the free compounds (A) or free compounds (B) or free compounds (C), respectively, where appropriate and expedient. The equivalent also applies to tautomers of compounds (A) or compounds (B) or compounds (C) and to their salts.

According to the invention the expression "combination" stands for the various combinations of compounds (A) and (B) and optionally (C), for example in a single "ready-mix" form, in a com- bined spray mixture composed from separate formulations of the single active compounds, such as a "tank-mix", and in a combined use of the single active ingredients when applied in a sequential manner, i.e. one after the other within a reasonably short period, such as a few hours or days. Preferably the order of applying the compounds (A) and (B) and optionally (C) is not essential for working the present invention.

In a further aspect, there is provided a composition comprising a combination according to this invention. Preferably the fungicidal composition comprises an agriculturally acceptable support, carrier or filler.

According to the invention, the term "support" denotes a natural or synthetic, organic or inorganic compound with which the active compound (A) and (B) and optionally (C) is combined or associated to make it easier to apply, notably to the parts of the plant. This support is thus generally inert and should be agriculturally acceptable. The support may be a solid or a liquid. Examples of suitable supports include clays, natural or synthetic silicates, silica, resins, waxes, solid fertilisers, water, alcohols, in particular butanol, organic solvents, mineral and plant oils and derivatives thereof. Mixtures of such supports may also be used.

The composition according to the invention may also comprise additional components. In particular, the composition may further comprise a surfactant. The surfactant can be an emulsifier, a dispersing agent or a wetting agent of ionic or non-ionic type or a mixture of such surfactants. Mention may be made, for example, of polyacrylic acid salts, lignosulphonic acid salts, phenol- sulphonic or naphthalenesulphonic acid salts, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, substituted phenols (in particular alkylphenols or arylphenols), salts of sulphosuccinic acid esters, taurine derivatives (in particular alkyl taurates), phosphoric esters of polyoxyethylated alcohols or phenols, fatty acid esters of polyols, and derivatives of the present compounds containing sulphate, sulphonate and phosphate functions. The presence of at least one surfactant is generally essential when the active compound and / or the inert support are water-insoluble and when the vector agent for the application is water. Preferably, surfactant content may be comprised from 5% to 40% by weight of the composition.

Colouring agents such as inorganic pigments, for example iron oxide, titanium oxide, ferrocy- anblue, and organic pigments such as alizarin, azo and metallophthalocyanine dyes, and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts can be used.

Optionally, other additional components may also be included, e.g. protective colloids, adhe- sives, thickeners, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active compounds can be combined with any solid or liquid additive, which complies with the usual formulation techniques.

In general, the composition according to the invention may contain from 0.05 to 99% by weight of active compounds, preferably from 10 to 70% by weight.

The combination or composition according to the invention can be used as such, in form of their formulations or as the use forms prepared therefrom, such as aerosol dispenser, capsule suspension, cold fogging concentrate, dustable powder, emulsifiable concentrate, emulsion oil in water, emulsion water in oil, encapsulated granule, fine granule, flowable concentrate for seed treatment, gas (under pressure), gas generating product, granule, hot fogging concentrate, mac- rogranule, microgranule, oil dispersible powder, oil miscible flowable concentrate, oil miscible liquid, paste, plant rodlet, powder for dry seed treatment, seed coated with a pesticide, soluble concentrate, soluble powder, solution for seed treatment, suspension concentrate (flowable concentrate), ultra low volume (ULV) liquid, ultra low volume (ULV) suspension, water dispersible granules or tablets, water dispersible powder for slurry treatment, water soluble granules or tablets, water soluble powder for seed treatment and wettable powder.

The treatment of plants and plant parts with the active compound combination according to the invention is carried out directly or by action on their environment, habitat or storage area by means of the normal treatment methods, for example by watering (drenching), drip irrigation, spraying, atomizing, broadcasting, dusting, foaming, spreading-on, and as a powder for dry seed treatment, a solution for seed treatment, a water-soluble powder for seed treatment, a water- soluble powder for slurry treatment, or by encrusting.

These combinations or compositions include not only compositions which are ready to be applied to the plant or seed to be treated by means of a suitable device, such as a spraying or dusting device, but also concentrated commercial compositions which must be diluted before application to the crop.

The active compounds within the combinations or compositions according to the invention have potent microbicide activity and can be employed for controlling undesired micro-organisms, such as fungi or bacteria, in crop protection or in the protection of materials.

Within the combinations or compositions according to the invention, fungicide compounds can be employed in crop protection for example for controlling Plasmodiophoromycetes, Oomy- cetes, Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes and Deuteromycetes.

The fungicide combinations or compositions according to the invention can be used to curatively or preventively control the phytopathogenic fungi of plants or crops. Thus, according to a further as- pect of the invention, there is provided a method for curatively or preventively controlling the phy- topathogenic fungi of plants or crops comprising the use of a fungicide combination or composition according to the invention by application to the seed, the plant or to the fruit of the plant or to the soil in which the plant is growing or in which it is desired to grow.

The combinations or composition of the invention is also suitable for the treatment of seeds. A large part of the damage caused by diseases on cultigens occurs by infestation of the seed during storage and after sowing the seed in the ground as well as during and after germination of the plants. This phase is especially critical since the roots and shoots of the growing plant are particularly sensitive and even a small amount of damage can lead to withering of the whole plant.

The method of treatment according to the invention may also be useful to treat propagation material such as tubers or rhizomes, but also seeds, seedlings or seedlings pricking out and plants or plants pricking out. This method of treatment can also be useful to treat roots. The method of treatment according to the invention can also be useful to treat the over-ground parts of the plant such as trunks, stems or stalks, leaves, flowers and fruit of the concerned plant.

Plants which can be treated in accordance with the invention include the following main crop plants: maize, soya bean, alfalfa, cotton, sunflower, Brassica oil seeds such as Brassica napus (e.g. canola, rapeseed), Brassica rapa, B.juncea (e.g. (field) mustard) and Brassica carinata, Arecaceae sp. (e.g. oilpalm, coconut), rice, wheat, sugar beet, sugar cane, oats, rye, barley, millet and sorghum, triticale, flax, nuts, grapes and vine and various fruit and vegetables from various botanic taxa, e.g. Rosaceae sp. (e.g. pome fruits such as apples and pears, but also stone fruits such as apricots, cherries, almonds, plums and peaches, and berry fruits such as strawberries, raspberries, red and black currant and gooseberry), Ribesioidae sp., Juglandaceae sp., Betulaceae sp., Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae sp. (e.g. olive tree), Actinidaceae sp., Lauraceae sp. (e.g. avocado, cinnamon, camphor), Musaceae sp. (e.g. banana trees and plantations), Rubiaceae sp. (e.g. coffee), Theaceae sp. (e.g. tea), Sterculiceae sp., Rutaceae sp. (e.g. lemons, oranges, mandarins and grapefruit); Solanaceae sp. (e.g. tomatoes, potatoes, peppers, capsicum, aubergines, tobacco), Liliaceae sp., Compositae sp. (e.g. lettuce, artichokes and chicory - including root chicory, endive or common chicory), Umbelliferae sp. (e.g. carrots, parsley, celery and celeriac), Cucurbitaceae sp. (e.g. cucumbers - including gherkins, pumpkins, watermelons, calabashes and melons), AUiaceae sp. (e.g. leeks and onions), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, pak choi, kohlrabi, radishes, horseradish, cress and Chinese cabbage), Leguminosae sp. (e.g. peanuts, peas, lentils and beans - e.g. common beans and broad beans), Chenopodiaceae sp. (e.g. Swiss chard, fodder beet, spinach, beetroot), Linaceae sp. (e.g. hemp), Cannabeacea sp. (e.g. cannabis), Malvaceae sp. (e.g. okra, cocoa), Papaveraceae (e.g. poppy), Asparagaceae (e.g. asparagus); useful plants and ornamental plants in the garden and woods including turf, lawn, grass and Stevia rebaudi- ana; and in each case genetically modified types of these plants.

Preferably plants which can be treated in accordance with the invention include cereals such as wheat, rye, barley and triticale, Cucurbitaceae sp. (e.g. cucumbers - including gherkins, pumpkins, watermelons, calabashes and melons), Solanaceae sp. (e.g. tomatoes, peppers, capsicum, aubergines), Rosaceae sp. (e.g. pome fruits such as apples and pears, but also berry fruits such as strawberries), Compositae sp. (e.g. lettuce, artichokes and chicory - including root chicory, endive or common chicory), Umbelliferae sp. (e.g. carrots, parsley, celery and celeriac), Cucurbitaceae sp. (e.g. cucumbers - including gherkins, pumpkins, watermelons, calabashes and melons), AUiaceae sp. (e.g. leeks and onions), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, pak choi, kohlrabi, radishes, horseradish, cress and Chinese cabbage), Leguminosae sp. (e.g. peanuts, peas, lentils and beans - e.g. common beans and broad beans), soya bean, grapes and vine, sugar beet and cutflowers.

In a preferred embodiment of the present application preferred plants which can be treated with an active compound combination comprising (A) proquinazid and (B) spiroxamine include cereals such as wheat, rye, barley and triticale, Cucurbitaceae sp. (e.g. cucumbers - including gherkins, pumpkins, watermelons, calabashes and melons), Solanaceae sp. (e.g. tomatoes, peppers, capsicum, aubergines), Rosaceae sp. (e.g. pome fruits such as apples and pears, but also berry fruits such as strawberries), Compositae sp. (e.g. lettuce, artichokes and chicory - including root chicory, endive or common chicory), Umbelliferae sp. (e.g. carrots, parsley, celery and celeriac), Cucurbitaceae sp. (e.g. cucumbers - including gherkins, pumpkins, watermelons, calabashes and melons), AUiaceae sp. (e.g. leeks and onions), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, pak choi, kohlrabi, radishes, horseradish, cress and Chinese cabbage), Leguminosae sp. (e.g. peanuts, peas, lentils and beans - e.g. common beans and broad beans), soya bean, grapes and vine, sugar beet and cutflowers.

In a preferred embodiment of the present application preferred plants which can be treated with an active compound combination comprising (A) proquinazid and (B) spiroxamine and (C) prothiocon- azole include cereals such as wheat, rye, barley and triticale.

The method of treatment according to the invention can be used in the treatment of genetically modified organisms, e.g. plants or seeds. Genetically modified plants are plants of which a heterologous gene encoding a protein of interest has been stably integrated into genome. The expression "heterologous gene encoding a protein of interest" essentially means genes which give the transformed plant new agronomic properties, or genes for improving the agronomic quality of the modified plant. The inventive method for controlling unwanted fungi can also be employed for protecting storage goods. Storage goods are understood to mean natural substances of vegetable or animal origin or processed products thereof which are of natural origin, and for which long-term protection is desired. Storage goods of vegetable origin, for example plants or plant parts, such as stems, leaves, tubers, seeds, fruits, grains, can be protected freshly harvested or after processing by (pre)drying, moistening, comminuting, grinding, pressing or roasting. Storage goods also include timber, both unprocessed, such as construction timber, electricity poles and barriers, or in the form of finished products, such as furniture. Storage goods of animal origin are, for example, hides, leather, furs and hairs. The inventive active ingredients may prevent adverse effects, such as rotting, decay, discoloration, discoloration or formation of mould. Preferably "storage goods" is understood to denote natural substances of vegetable origin and their processed forms, more preferably fruits and their processed forms, such as pomes, stone fruits, soft fruits and citrus fruits and their processed forms.

Non-limiting examples of pathogens of fungal diseases which can be treated in accordance with the invention include: diseases caused by powdery mildew pathogens, for example Blumeria species, for example Blume- ria graminis; Podosphaera species, for example Podosphaera leucotricha; Sphaerotheca species, for example Sphaerotheca fuliginea; Uncinula species, for example Uncinula necator; Microsphae- ra species, for example Microsphaera diffusa; Leveillula species, for example Leveillula taurica; Erysiphe species, for example Erysiphe cichoracearum; diseases caused by rust disease pathogens, for example Gymnosporangium species, for example Gymnosporangium sabinae; Hemileia species, for example Hemileia vastatrix; Phakopsora species, for example Phakopsora pachyrhizi and Phakopsora meibomiae; Puccinia species, for example Puccinia recondite, P. triticina, P. graminis or P. striiformis; Uromyces species, for example Uro- myces appendiculatus; diseases caused by pathogens from the group of the Oomycetes, for example Albugo species, for example Algubo Candida; Bremia species, for example Bremia lactucae; Peronospora species, for example Peronospora pisi or P. brassicae; Phytophthora species, for example Phytophthora infestans; Plasmopara species, for example Plasmopara viticola; Pseudoperonospora species, for example Pseudoperonospora humuli or Pseudoperonospora cubensis; Pythium species, for example Pythium ultimum; leaf blotch diseases and leaf wilt diseases caused, for example, by Alternaria species, for example Alternaria solani; Cercospora species, for example Cercospora beticola; Cladiosporium species, for example Cladiosporium cucumerinum; Cochliobolus species, for example Cochliobolus sativus (co- nidia form: Drechslera, Syn: Helminthosporium), Cochliobolus miyabeanus; Colletotrichum species, for example Colletotrichum lindemuthanium; Cycloconium species, for example Cycloconium ole- aginum; Diaporthe species, for example Diaporthe citri; Elsinoe species, for example Elsinoe faw- cettii; Gloeosporium species, for example Gloeosporium laeticolor; Glomerella species, for example Glomerella cingulata; Guignardia species, for example Guignardia bidwelli; Leptosphaeria species, for example Leptosphaeria maculans, Leptosphaeria nodorum; Magnaporthe species, for example Magnaporthe grisea; Marssonia species, for example Marssonia coronaria; Microdochium species, for example Microdochium nivale; Mycosphaerella species, for example Mycosphaerella gramini- cola, M. arachidicola and M. fijiensis; Phaeosphaeria species, for example Phaeosphaeria nodorum; Pyrenophora species, for example Pyrenophora teres, Pyrenophora tritici repentis; Ramularia species, for example Ramularia collo-cygni, Ramularia areola; Rhynchosporium species, for example Rhynchosporium secalis; Septoria species, for example Septoria apii, Septoria lycopersii; Typhula species, for example Typhula incarnata; Venturia species, for example Venturia inaequalis; root and stem diseases caused, for example, by Corticium species, for example Corticium gramine- arum; Fusarium species, for example Fusarium oxysporum; Gaeumannomyces species, for example Gaeumannomyces graminis; Rhizoctonia species, such as, for example Rhizoctonia solani; Saro- cladium diseases caused for example by Sarocladium oryzae; Sclerotium diseases caused for example by Sclerotium oryzae; Tapesia species, for example Tapesia acuformis; Thielaviopsis species, for example Thielaviopsis basicola; ear and panicle diseases (including corn cobs) caused, for example, by Alternaria species, for example Alternaria spp.; Aspergillus species, for example Aspergillus flavus; Cladosporium species, for example Cladosporium cladosporioides; Claviceps species, for example Claviceps purpurea; Fusarium species, for example Fusarium culmorum; Gibberella species, for example Gibberella ze- ae; Monographella species, for example Monographella nivalis; Septoria species, for example Septoria nodorum; diseases caused by smut fungi, for example Sphacelotheca species, for example Sphacelotheca reili- ana; Tilletia species, for example Tilletia caries, T. controversy Urocystis species, for example Urocystis occulta; Ustilago species, for example Ustilago nuda, U. nuda tritici; fruit rot caused, for example, by Aspergillus species, for example Aspergillus flavus; Botrytis species, for example Botrytis cinerea; Penicillium species, for example Penicillium expansum and P. purpurogenum; Sderotinia species, for example Sderotinia sclerotiorum; Verticilium species, for example Verticilium alboatrum; seed and soilborne decay, mould, wilt, rot and damping-off diseases caused, for example, by Alternaria species, caused for example by Alternaria brassicicola; Aphanomyces species, caused for example by Aphanomyces euteiches; Ascochyta species, caused for example by Ascochyta lentis; As- pergillus species, caused for example by Aspergillus flavus; Cladosporium species, caused for example by Cladosporium herbarum; Cochliobolus species, caused for example by Cochliobolus sa- tivus; (Conidiaform: Drechslera, Bipolaris Syn: Helminthosporium); Colletotrichum species, caused for example by Colletotrichum coccodes; Fusarium species, caused for example by Fusarium cul- morum; Gibberella species, caused for example by Gibberella zeae; Macrophomina species, caused for example by Macrophomina phaseolina; Monographella species, caused for example by Monographella nivalis; Penicillium species, caused for example by Penicillium expansum; Phoma species, caused for example by Phoma lingam; Phomopsis species, caused for example by Phomop- sis sojae; Phytophthora species, caused for example by Phytophthora cactorum; Pyrenophora species, caused for example by Pyrenophora graminea; Pyricularia species, caused for example by Pyricularia oryzae; Pythium species, caused for example by Pythium ultimum; Rhizoctonia species, caused for example by Rhizoctonia solani; Rhizopus species, caused for example by Rhizopus oryzae; Sclerotium species, caused for example by Sclerotium rolfsii; Septoria species, caused for example by Septoria nodorum; Typhula species, caused for example by Typhula incarnata; Verticilli- um species, caused for example by Verticillium dahliae; cancers, galls and witches' broom caused, for example, by Nectria species, for example Nectria gal- ligena; wilt diseases caused, for example, by Monilinia species, for example Monilinia laxa; leaf blister or leaf curl diseases caused, for example, by Exobasidium species, for example Exobasid- ium vexans;

Taphrina species, for example Taphrina deformans; decline diseases of wooden plants caused, for example, by Esca disease, caused for example by Phaemoniella damydospora, Phaeoacremonium aleophilum and Fomitiporia mediterranea; Eutypa dyeback, caused for example by Eutypa lata ; Ganoderma diseases caused for example by Ganoderma boninense; Rigidoporus diseases caused for example by Rigidoporus lignosus; diseases of flowers and seeds caused, for example, by Botrytis species, for example Botrytis cinerea; diseases of plant tubers caused, for example, by Rhizoctonia species, for example Rhizoctonia solani; Helminthosporium species, for example Helminthosporium solani;

Club root caused, for example, by Plasmodiophora species, for example Plamodiophora brassicae; diseases caused by bacterial pathogens, for example Xanthomonas species, for example Xanthomo- nas campestris pv. oryzae; Pseudomonas species, for example Pseudomonas syringae pv. lachry- mans; Erwinia species, for example Erwinia amylovora.

The following diseases of soya beans can be controlled with preference:

Fungal diseases on leaves, stems, pods and seeds caused, for example, by Altemaria leaf spot {Altemaria spec, atrans tenuissima), Anthracnose (Colletotrichum gloeosporoides dematium var. trun- catum), brown spot (Septoria glycines), cercospora leaf spot and blight (Cercospora kikuchii), cho- anephora leaf blight (Choanephora infundibulifera trispora (Syn.)), dactuliophora leaf spot (Dac- tuliophora glycines), downy mildew (Peronospora manshurica), drechslera blight (Drechslera glycini), frogeye leaf spot (Cercospora sojina), leptosphaerulina leaf spot (LeptosphaeruUna trifolii), phyllostica leaf spot (Phyllosticta sojaecola), pod and stem blight (Phomopsis sojae), powdery mildew (Microsphaera diffusa), pyrenochaeta leaf spot (Pyrenochaeta glycines), rhizoctonia aerial, foliage, and web blight (Rhizoctonia solani), rust (Phakopsora pachyrhizi, Phakopsora meibomiae), scab (Sphaceloma glycines), stemphylium leaf blight (Stemphylium botryosum), target spot (Corynespora cassiicola).

Fungal diseases on roots and the stem base caused, for example, by black root rot (Calonectria crot- alariae), charcoal rot (Macrophomina phaseolina), fusarium blight or wilt, root rot, and pod and collar rot (Fusarium oxysporum, Fusarium orthoceras, Fusarium semitectum, Fusarium equiseti), my- coleptodiscus root rot (Mycoleptodiscus terrestris), neocosmospora (Neocosmospora vasinfecta), pod and stem blight (Diaporthe phaseolorum), stem canker (Diaporthe phaseolorum var. caulivora), phytophthora rot (Phytophthora megasperma), brown stem rot (Phialophora gregata), pythium rot (Pythium aphanidermatum, Pythium irregulare, Pythium debaryanum, Pythium myriotylum, Pythium ultimum), rhizoctonia root rot, stem decay, and damping-off (Rhizoctonia solani), sclerotinia stem decay (Sclerotinia sclerotiorum), sclerotinia southern blight (Sclerotinia rolfsii), thielaviopsis root rot (Thielaviopsis basicola).

Preferably, the active compound combination according to the present invention allows excellent powdery mildew control.

Therefore, in a particular preferred embodiment diseases of plants or crops that can be controlled by the method according to the invention are diseases caused by powdery mildew pathogens, for example Blumeria species, for example Blumeria graminis; Podosphaera species, for example Podosphaera leucotricha; Sphaerotheca species, for example Sphaerotheca fuliginea; Uncinula species, for example Uncinula necator; Microsphaera species, for example Microsphaera diffusa; Leveillula species, for example Leveillula taurica; Erysiphe species, for example Erysiphe cichoracearum Particularly preferred, the active ingredient combinations or compositions according to the invention are suitable for controlling the following plant diseases:

Blumeria (old name: Erysiphe) graminis (powdery mildew) on cereals (e.g. wheat or barley); Ery- siphe spp. (powdery mildew) on sugar beet (E. betae), vegetables (e.g. E. pisi), such as cucumber, gourd, muskmelon (cantaloupe), pumpkin, and squash species (e.g. E. cichoracearum) and cabbage species, such as red cabbage, broccoli, brussels sprouts, cauliflower (e.g. E. cruciferarum); and tomato (Erysiphe lycopersici), and pepper, eggplant, artischoke (Leveillula taurica), Microsphaera diffusa (powdery mildew) on soybeans; Podosphaera spp. (powdery mildew) on Rosaceae, hops, pomaceaus fruit and soft fruit, e.g. P. leucotricha on apple; Uncinula (syn. Erysiphe) necator {powdery mildew, anamorph: Oidium tuckeri) on grapevines; Sphaerotheca fuliginea (powdery mildew) on cucumber species.

In addition to such excellent control of diseases caused by powdery mildew pathogens the active compound combination or compositions according to the invention comprising prothioconazole are particularly suitable for controlling diseases on cereals. Such active compound combination or compositions according to the invention comprising prothioconazole are active compound combination or compositions comprising (A) proquinazid and (B) spiroxamine and (C) prothioconazole.

Such active compound combination or compositions according to the invention comprising prothioconazole are particularly suitable for controlling the following diseases on cereals, in particular on wheat or barley:

Mycosphaerella species, for example Mycosphaerella graminicola (synonym Septoria tritici), Puc- cinia species, for example Puccinia recondite, P. triticina, P. graminis or P. striiformis; Lepto- sphaeria species, for example Leptosphaeria nodorum; Pyrenophora species, for example Pyre- nophora teres, Pyrenophora tritici repentis, Pyrenophora graminea; Ramularia species, for example Ramularia collo-cygni, Gaeumannomyces species, for example Gaeumannomyces graminis; Fusarium species, for example Fusarium culmorum; Gibberella species, for example Gibberella ze- ae; Monographella species, for example Monographella nivalis; Septoria species, for example Septoria nodorum; Cochliobolus species, caused for example by Cochliobolus sativus; (Conidiaform: Drechslera, Bipolaris Syn: Helminthosporium) Rhynchosporium species, for example Rhyn- chosporium secalis.

With the active compound combination or compositions according to the invention comprising prothioconazole in particular improved control of Mycosphaerella species, for example Mycosphaerella graminicola (synonym Septoria tritici) can be obtained. The active compound combination or compositions according to the invention allow a broadening of the spectrum of fungicidal activity to other phytopathogens against the individual compounds are less effective or totally ineffective: Additionally, although achieving adequate fungicidal control a reduction in the rate of application of the active ingredients can be achieved by the active compound combination or compositions according to the invention. Such reduction in the rate of application of the individual active ingredients reduces the risk of resistances against such individual active ingredients.

In particular the application rate of at least one of the active ingredient of the active compound combination or compositions according to the invention can be reduced while the excellent control of diseases caused by powdery mildew pathogens and/or other diseases on cereals, such as diseases caused by Mycosphaerella species, for example Mycosphaerella graminicola (synonym Septoria tritici) remain equally good.

The method of treatment according to the invention also provides the use or application of compounds (A) and (B) and optionally (C) in a simultaneous, separate or sequential manner. If the single active ingredients are applied in a sequential manner, i.e. at different times, they are applied one after the other within a reasonably short period, such as a few hours or days. Preferably the order of applying the compounds (A) and (B) and optionally (C) is not essential for working the present invention.

When using active ingredients as fungicides, the application rates can be varied within a relatively wide range, depending on the kind of application. The application rate of the inventive active ingredients is generally and advantageously

• in the case of treatment of plant parts, for example leaves: from 0.1 to 10 000 g/ha, preferably from 10 to 1000 g/ha, more preferably from 10 to 800 g/ha, even more preferably from 50 to 300 g/ha (in the case of application by watering or dripping, it is even possible to reduce the application rate, especially when inert substrates such as rock- wool or perlite are used);

• in the case of seed treatment: from 2 to 200 g per 100 kg of seed, preferably from 3 to 150 g per 100 kg of seed, more preferably from 2.5 to 25 g per 100 kg of seed, even more preferably from 2.5 to 12.5 g per 100 kg of seed;

• in the case of soil treatment: from 0.1 to 10 000 g/ha, preferably from 1 to 5000 g/ha.

In case of treatment of plant parts the application rate of the inventive active ingredients is preferably • from 20 to 100 g/ha proquinazid (compound (A)), more preferably from 30 to 70 g/ha proquinazid, most preferably from 35 to 60 g/ha proquinazid;

• from 100 to 350 g/ha spiroxamine (compound (B)), more preferably from 150 to 300 g/ha spiroxamine, most preferably from 180 to 270 g/ha spiroxamine;

• from 100 to 250 g/ha prothioconazole (Compound (C)), more preferably from 125 to 225 g/ha prothioconazole, most preferably from 140 to 220 g/ha prothioconazole.

It is particularly preferred that the application rate for spiroxamine (compound (B)) is below 300 g/ha, more preferably lower that 275 g/ha.

Treatment of seeds

The combinations or compositions of the invention are particularly suitable for the treatment of seeds.

The invention comprises a procedure in which the seed is treated at the same time with a compound (A) and a compound (B) and optionally a compound (C). It further comprises a method in which the seed is treated with compound (A) and compound (B) and optionally compound (C) sequentially or separately, i.e. at different times. If the single active ingredients are applied in a sequential / separate manner, i.e. at different times, they are applied one after the other within a reasonably short period, such as a few hours or days. Preferably the order of applying the compounds (A) and (B) and optionally (C) is not essential for working the present invention.

The invention also comprises a seed, which has been treated with compound (A) and compound (B) and optionally compound (C) at the same time. The invention also comprises a seed, which has been treated with compound (A) and compound (B) and optionally compound (C) sequentially or separately, i.e. at different times. For the seed treated with compound (A) and compound (B) and optionally compound (C) sequentially or separately, the active ingredients can be applied in separate layers. These layers can optionally be separated by an additional layer that may or may not contain an active ingredient. According to a preferred embodiment of the present invention, seed which has been treated with compound (A) and compound (B) and optionally compound (C) at the same time or at different times refers to seed that still comprises an amount of compound (A) and compound (B) and optionally compound (C).

A large part of the damage to crop plants caused by harmful organisms is triggered by the infection of the seed during storage or after sowing, and also during and after germination of the plant. This phase is particularly critical since the roots and shoots of the growing plant are particularly sensitive, and even minor damage may result in the death of the plant. There is therefore a great interest in protecting the seed and the germinating plant by using appropriate compositions.

The control of phytopathogenic fungi by treating the seed of plants has been known for a long time and is the subject of constant improvements. However, the treatment of seed entails a series of problems which cannot always be solved in a satisfactory manner. For instance, it is desirable to develop methods for protecting the seed and the germinating plant, which dispense with, or at least significantly reduce, the additional deployment of crop protection compositions after planting or after emergence of the plants. It is also desirable to optimize the amount of the active ingredient used so as to provide the best possible protection for the seed and the germinating plant from attack by phytopathogenic fungi, but without damaging the plant itself by the active ingredient employed. In particular, methods for the treatment of seed should also take account of the intrinsic fungicidal properties of transgenic plants in order to achieve optimal protection of the seed and the germinating plant with a minimum expenditure of crop protection compositions.

The present invention therefore also relates to a method for protection of seed and germinating plants from attack by phytopathogenic fungi, by treating the seed with an inventive composition. The invention likewise relates to the use of the inventive compositions for treatment of seed to protect the seed and the germinating plant from phytopathogenic fungi. The invention further relates to seed which has been treated with an inventive composition for protection from phytopathogenic fungi.

The control of phytopathogenic fungi which damage plants post-emergence is effected primarily by treating the soil and the above-ground parts of plants with crop protection compositions. Owing to the concerns regarding a possible influence of the crop protection compositions on the environment and the health of humans and animals, there are efforts to reduce the amount of active ingredients deployed.

One of the advantages of the present invention is that the particular systemic properties of the inventive active ingredients and compositions mean that treatment of the seed with these active ingredients and compositions not only protects the seed itself, but also the resulting plants after emergence, from phytopathogenic fungi. In this way, the immediate treatment of the crop at the time of sowing or shortly thereafter can be dispensed with.

It is likewise considered to be advantageous that the inventive active ingredients or compositions can especially also be used with transgenic seed, in which case the plant growing from this seed is capable of expressing a protein which acts against pests. By virtue of the treatment of such seed with the inventive active ingredients or compositions, merely the expression of the protein, for example an insecticidal protein, can control certain pests. Surprisingly, a further synergistic effect can be observed in this case, which additionally increases the effectiveness for protection against attack by pests.

The inventive compositions are suitable for protecting seed of any plant variety which is used in agriculture, in greenhouses, in forests or in horticulture and viticulture. In particular, this is the seed of cereals (such as wheat, barley, rye, triticale, sorghum/millet and oats), maize, cotton, soya beans, rice, potatoes, sunflower, bean, coffee, beet (for example sugar beet and fodder beet), peanut, oilseed rape, poppy, olive, coconut, cocoa, sugar cane, tobacco, vegetables (such as tomato, cucumbers, onions and lettuce), turf and ornamentals (see also below). The treatment of the seed of cereals (such as wheat, barley, rye, triticale and oats), maize and rice is of particular significance.

As also described below, the treatment of transgenic seed with the inventive active ingredients or compositions is of particular significance. This relates to the seed of plants containing at least one heterologous gene. Definition and examples of suitable heterologous genes are given below.

In the context of the present invention, the inventive composition is applied to the seed alone or in a suitable formulation. Preferably, the seed is treated in a state in which it is sufficiently stable for no damage to occur in the course of treatment. In general, the seed can be treated at any time between harvest and sowing. It is customary to use seed which has been separated from the plant and freed from cobs, shells, stalks, coats, hairs or the flesh of the fruits. For example, it is possible to use seed which has been harvested, cleaned and dried down to a moisture content of less than 15 % by weight. Alternatively, it is also possible to use seed which, after drying, for example, has been treated with water and then dried again.

When treating the seed, care must generally be taken that the amount of the inventive composition applied to the seed and/or the amount of further additives is selected such that the germination of the seed is not impaired, or that the resulting plant is not damaged. This has to be borne in mind in particular in the case of active ingredients which can have phytotoxic effects at certain application rates.

The inventive compositions can be applied directly, i.e. without containing any other components and without having been diluted. In general, it is preferable to apply the compositions to the seed in the form of a suitable formulation. Suitable formulations and methods for seed treatment are known to those skilled in the art and are described, for example, in the following documents: US 4,272,417, US 4,245,432, US 4,808,430, US 5,876,739, US 2003/0176428 Al, WO 2002/080675, WO 2002/028186.

The active ingredients usable in accordance with the invention can be converted to the customary seed dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries or other coating compositions for seed, and also ULV formulations.

These formulations are prepared in a known manner, by mixing the active ingredients with customary additives, for example customary extenders and also solvents or diluents, dyes, wetting agents, dispersants, emulsifiers, antifoams, preservatives, secondary thickeners, adhesives, gib- berellins and also water.

Useful dyes which may be present in the seed dressing formulations usable in accordance with the invention are all dyes which are customary for such purposes. It is possible to use either pigments, which are sparingly soluble in water, or dyes, which are soluble in water. Examples include the dyes known by the names Rhodamine B, C.I. Pigment Red 112 and C.I. Solvent Red 1.

Useful wetting agents which may be present in the seed dressing formulations usable in accordance with the invention are all substances which promote wetting and which are conventionally used for the formulation of active agrochemical ingredients. Preference is given to using alkyl naphthalenesulphonates, such as diisopropyl or diisobutyl naphthalenesulphonates.

Useful dispersants and/or emulsifiers which may be present in the seed dressing formulations usable in accordance with the invention are all nonionic, anionic and cationic dispersants conventionally used for the formulation of active agrochemical ingredients. Usable with preference are nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants. Suitable nonionic dispersants include especially ethylene oxide/propylene oxide block polymers, al- kylphenol polyglycol ethers and tristryrylphenol polyglycol ether, and the phosphated or sul- phated derivatives thereof. Suitable anionic dispersants are especially lignosulphonates, poly- acrylic acid salts and arylsulphonate/formaldehyde condensates.

Antifoams which may be present in the seed dressing formulations usable in accordance with the invention are all foam-inhibiting substances conventionally used for the formulation of active agrochemical ingredients. Silicone antifoams and magnesium stearate can be used with preference.

Preservatives which may be present in the seed dressing formulations usable in accordance with the invention are all substances usable for such purposes in agrochemical compositions. Examples include dichlorophene and benzyl alcohol hemiformal. Secondary thickeners which may be present in the seed dressing formulations usable in accordance with the invention are all substances usable for such purposes in agrochemical compositions. Preferred examples include cellulose derivatives, acrylic acid derivatives, xanthan, modified clays and finely divided silica.

Adhesives which may be present in the seed dressing formulations usable in accordance with the invention are all customary binders usable in seed dressing products. Preferred examples include polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose.

The gibberellins which may be present in the seed dressing formulations usable in accordance with the invention may preferably be gibberellins Al, A3 (= gibberellic acid), A4 and A7; particular preference is given to using gibberellic acid. The gibberellins are known (cf. R. Wegler "Chemie der Pflanzenschutz- und Schadlingsbekampfungsmittel" [Chemistry of the Crop Protection Compositions and Pesticides], vol. 2, Springer Verlag, 1970, p. 401-412).

The seed dressing formulations usable in accordance with the invention can be used, either directly or after previously having been diluted with water, for the treatment of a wide range of different seed, including the seed of transgenic plants. In this case, additional synergistic effects may also occur in interaction with the substances formed by expression.

For treatment of seed with the seed dressing formulations usable in accordance with the invention, or the preparations prepared therefrom by adding water, all mixing units usable customarily for the seed dressing are useful. Specifically, the procedure in the seed dressing is to place the seed into a mixer, to add the particular desired amount of seed dressing formulations, either as such or after prior dilution with water, and to mix everything until the formulation is distributed homogeneously on the seed. If appropriate, this is followed by a drying process.

According to another aspect of the present invention, in the combination or composition according to the invention, the compound ratio A/B, A/C or A B/C may be advantageously chosen so as to produce a synergistic effect. A synergistic effect of fungicides is always present when the fungicidal activity of the active compound combinations exceeds the total of the activities of the active compounds when applied individually. The expected activity for a given combination of two or three active compounds can be calculated as follows (cf. Colby, S.R., "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22).

The latter article mentions the formula for combinations of 2 active compounds:

X^■ Y

E, = X + Y and the formula for a combination of 3 active compounds:

wherein

X denotes the efficacy when using active compound A at an application rate of m ppm (or g ha),

Y denotes the efficacy when using active compound B (or C) at an application rate of n ppm

(or g/ha),

Z denotes the efficacy when using active compound C at an application rate of r ppm (or g ha),

Ei denotes the efficacy when using active compounds A and B at application rates of m and n ppm (or g/ha), and

E2 denotes the efficacy when using active compounds A and B and C at application rates of m and n and r ppm (or g/ha),

The term "synergistic effect" also means the effect defined by application of the Tammes method, "Isoboles, a graphic representation of synergism in pesticides", Netherlands Journal of Plant Pathology, 70(1964), pages 73-80.

Here, the efficacy is determined in %. 0 % means an efficacy which corresponds to that of the control, whereas an efficacy of 100 % means that no infection is observed.

If the actual fungicidal action exceeds the calculated value, the action of the combination is superad- ditive, i.e. a synergistic effect is present. In this case, the actually observed efficacy must exceed the value calculated using the above formula for the expected efficacy (Ei or E2).

The invention is illustrated by the following examples. However the invention is not limited to the examples. Examples

Example 1 :

The advanced fungicidal activity of the active compound combinations according to the invention is evident from the example below. While the individual active compounds exhibit weaknesses with regard to the fungicidal activity, the combinations have an activity which exceeds a simple addition of activities.

A synergistic effect of fungicides is always present when the fungicidal activity of the active compound combinations exceeds the total of the activities of the active compounds when applied individually. The expected activity for a given combination of two active compounds can be calculated as follows (cf. Colby, S.R., "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22):

If

X is the efficacy when active compound A is applied at an application rate of m ppm (or g ha),

Y is the efficacy when active compound B is applied at an application rate of n ppm (or g ha),

E is the efficacy when the active compounds A and B are applied at application rates of m and n ppm (or g/ha), respectively, and then

E _{= X +} Y - ^∑

100

The degree of efficacy, expressed in % is denoted. 0 % means an efficacy which corresponds to that of the control while an efficacy of 100 % means that no disease is observed.

If the actual fungicidal activity exceeds the calculated value, then the activity of the combination is superadditive, i.e. a synergistic effect exists. In this case, the efficacy which was actually observed must be greater than the value for the expected efficacy (E) calculated from the abovementioned formula.

A further way of demonstrating a synergistic effect is the method of Tammes (cf. "Isoboles, a graphic representation of synergism in pesticides" in Neth. J. Plant Path., 1964, 70, 73-80). The invention is illustrated by the following examples. However the invention is not limited to the examples.

Example: in vivo preventive Blumeria test (wheat)

Solvent: 49 parts by weight of N,N-dimethylacetamide

Emulsifier: 1 part by weight of alkylaryl polyglycol ether

To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration. To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application. After the spray coating has been dried, the plants are dusted with spores of Blumeria graminis f.sp. tritici. The plants are placed in the greenhouse at a temperature of approximately 18°C and a relative atmospheric humidity of approximately 80% to promote the development of mildew pustules.

The test is evaluated 7 days after the inoculation. 0%> means an efficacy which corresponds to that of the untreated control, while an efficacy of 100%> means that no disease is observed. The table below clearly shows that the observed activity of the active compound combination according to the invention is greater than the calculated activity, i.e. a synergistic effect is present.

Tab. 1 : in vivo preventive Blumeria test (wheat)

found = activity found

calc. = activity calculated using Colby's formula

The results show that the mixture of proqinazid and sprioxamine achieved an excellent and pected level of powdery mildew control. Example 2:

In field experiments the performances against Powdery mildew (Erysiphe graminis), Septoria leaf blotch {Septoria tritici) and the effects on yield of the fungicide combinations PQA+PTZ+SPX 400 EC (active substances proquinazid + prothioconazole + spiroxamine), Input^® 460 EC (active substances prothioconazole + spiroxamine), the tank mixtures Proline^® 250 EC + Talius^® 200 EC (active substances prothioconazole + proquinazid) as well as Talius^® 200 EC + Impulse^® 500 EC (proquinazid + spiroxamine) were compared.

Field trials in winter wheat were conducted in 2013 in cereal growing regions of Germany (Mecklenburg- Vorpommern, / n=2 trials; Hessen / n=l trial). The experiments were carried out according to approved guidelines (EPPO), respectively Bayer in-house guidelines which mostly follow the EPPO guidelines. The trials were carried out in four replicates with randomized plots.

The products were applied once. The treatment was made in May at the elongation stage (BBCH 31-32). The spray volumes were in line with farmers' practice, varying from 200-300 1/ha (Tab. 2).

Tab. 2: Field trials in winter wheat (Germany; 2013)

Fertilization, herbicide, plant growth regulator application was carried out according to local agricultural practice. Trials were harvested in August (92-97 days after application of the products in BBCH 31-32).

Detailed information about the products tested is shown in Tab. 3.

Tab. 3: Products tested Product FormulaDose rate Active ingredients Content Content tion type 1 / ha g a.i / 1 g a.i / ha

PQA+PTZ 400 EC 1,25 Proquinazid (PQA) 40 50

+SPX

Prothioconazole (PTZ) 160 200

Spiroxamine (SPX) 200 250

Input^® 460 EC 1,25 Prothioconazole 160 200

Spiroxamine 300 375

Proline^® 250 EC 0,80 Prothioconazole 250 200

+ Talius^® 200 EC + 0,25 Proquinazid 200 50

Talius^® 200 EC 0,25 Proquinazid 200 50

+ Impulse^® 500 EC + 0,50 Spiroxamine 500 250

Results

An overview of the efficacy results, with the assessment (disease severity in untreated control > 5 %) made on the most relevant leaves of each trial (E. graminis: 17-48 days after application, S. tritici: 17-36 days after application), is given in Tab. 4.

Tab. 4: Efficacy against Erysiphe graminis and Septoria tritici; yield data

S. tritici (n=3) 15,5 82,8 76,0 80,4 39,3

Yield (n=3) 83,6 110,0 109,3 109,7 107,9

Data show that 50 g proquinazid/ha, added to 200 g prothioconazole/ha and 250 g spirox- amine/ha (ratio 1 : 4 : 5 ), clearly bring an additional control of powdery mildew (Erysiphe graminis) and Septoria tritici.

The results clearly indicate that the new combination pro- quinazid + prothioconazole + spiroxamine 400 EC, providing 50 g/ha proquinazid + 200 g/ha prothioconazole and a dose of 250 g/ha spiroxamine, achieved an excellent level of disease control (Powdery mildew and Septoria tritici) superior to the full label dose of the commercial products Input^® 460 EC, providing 200 g/ha prothioconazole and 375 g/ha spiroxamine and to the tank mixture of Proline^® 250 EC + Talius^® 200 EC, providing 200 g prothioconazole and 50 g/ha proquinazid. The effect of the new combination proquinazid + prothioconazole + spiroxamine 400 EC, providing 50 g/ha proquinazid + 200 g/ha prothioconazole and a dose of 250 g/ha spiroxamine, on disease control and on yield was also superior over the effect of the tank mixture Talius^® 200 EC + Impulse^® 500 EC (50 g/ha proquinazid added to 250 g/ha spiroxamine).

The results clearly indicate that the new three-way mixture proquinazid + prothioconazole + spiroxamine 400 EC achieved an excellent level of performance (disease control and yield) superior to the full label dose of tested the commercial products (Input^® 460 EC, Proline^® 250 EC + Talius^® 200 EC) as well as over the tank mixture Talius^® 200 EC + Impulse^® 500 EC.

Example 3: in vivo preventive Pyrenoyhora teres test (barley)

Solvent: 49 parts by weight of N,N-dimethylacetamide

Emulsifier: 1 part by weight of alkylaryl polyglycol ether

To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration. To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application. After the spray coating has been dried, the plants are sprayed with a spore suspension of Pyrenophora teres. The plants remain for 48 hours in an incubation cabinet at approximately 20°C and a relative atmospheric humidity of approximately 100%. The plants are placed in the greenhouse at a temperature of approximately 20°C and a relative atmospheric humidity of approximately 80%. The test is evaluated 8 days after the inoculation. 0%> means an efficacy which corresponds to that of the untreated control, while an efficacy of 100%) means that no disease is observed.

Table 5: in vivo preventive Pyrenophora teres test (barley)

The application rates 400 + 750 + 100 ppm of the active compounds correspond to 200 + 375 + 50 g a.i./ha and the application rates 400 + 500 + 100 ppm of the active compounds correspond to 200 + 250 + 50 g a.i./ha.

Example 4: in vivo preventive Puccinia triticina test (wheat)

Solvent: 49 parts by weight of N,N-dimethylacetamide

Emulsifier: 1 part by weight of alkylaryl polyglycol ether

To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration. To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application. After the spray coating has been dried, the plants are sprayed with a spore suspension of Puccinia triticina. The plants remain for 48 hours in an incubation cabinet at approximately 20°C and a relative atmospheric humidity of approximately 100%). The plants are placed in the greenhouse at a temperature of approximately 20°C and a relative atmospheric humidity of approximately 80%>. The test is evaluated 8 days after the inoculation. 0%) means an efficacy which corresponds to that of the untreated control, while an efficacy of 100%) means that no disease is observed.

Table 6: in vivo preventive Puccinia triticina test (wheat)

Combination of active compound: Rate of application of acEfficacy tive compound in ppm in %

Prothioconazole + Spiroxamine + Proquinazid 400 + 750 + 100 100 Prothioconazole + Spiroxamine + Proquinazid 400 + 500 + 100 100

Example 5: in vivo preventive Fusarium sraminearum test (barley)

Solvent: 49 parts by weight of N,N-dimethylacetamide

Emulsifier: 1 part by weight of alkylaryl polyglycol ether

To produce a suitable preparation of active compound, 1 part by weight of active compound or active compound combination is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration. To test for preventive activity, young plants are sprayed with the preparation of active compound or active compound combination at the stated rate of application. After the spray coating has been dried, the plants are slightly injured by using a sandblast and afterwards they are sprayed with a conidia suspension of Fusarium graminearum. The plants are placed in the greenhouse under a translucent incubation cabinet at a temperature of approximately 25°C and a relative atmospheric humidity of approximately 100%. The test is evaluated 5 days after the inoculation. 0% means an efficacy which corresponds to that of the untreated control, while an efficacy of 100% means that no disease is observed.

Table 7: in vivo preventive Fusarium sraminearum test (barley)

Examples 3, 4 and 5 show that surprisingly in the ternary mixture according to the invention the weight ratio of proquinazid to spriroxamine and therewith the application rate of spriox- amine and the active ingredients in total can be significantly reduced without losing any efficacy.

Claims

Claims:

1. Active compound combination comprising

(A) proquinazid

and

(B) spiroxamine and optionally

(C) prothioconazole wherein the weight ratio of A:B is from 1:1 to 1:8.

2. Combination according to claim 1 wherein the weight ratio of A:B is from 1:1 to 1:7, preferably from 1 :2 to 1 :6, more preferably from 1 :3 to 1 :6 and the weight ratio of A:C is from 1:1 to 1:20, preferably from 1:1 to 1:10, more preferably from 1:2 to 1:6.

3. Active compound combination according to claim 1 comprising (A) proquinazid and (B) spiroxamine wherein the weight ratio of A:B is from 1:1 to 1:7, preferably from 1 :2 to 1 :6, more preferably from 1 :3 to 1 :6.

4. Active compound combination according to claim 1 comprising (A) proquinazid, (B) spiroxamine and (C) prothioconazole wherein the weight ratio of A:B is from 1:1 to 1:7, preferably from 1 :2 to 1 :6, more preferably from 1 :3 to 1 :6 and the weight ratio of A:C is from 1:1 to 1:20, preferably from 1:1 to 1:10, more preferably from 1:2 to 1:6.

5. Active compound combination according to claim 1 comprising (A) proquinazid, (B) spiroxamine and (C) prothioconazole wherein the weight ratio of A:B is from 1:1 to 1:7, preferably from 1 :2 to 1 :6, more preferably from 1 :3 to 1 :6 and the weight ratio of A:C is from 1 : 1 to 1 :20, preferably from 1:1 to 1:10, more preferably from 1 :2 to 1:6 and the weight ratio of B:C is from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 3:1 to 1:3.

6. Active compound combination according to any of claims 1 to 5 wherein the application rate of compound (B) is below 300 g/ha.

7. A composition comprising a combination according to claims 1 to 6.

8. A composition according to claim 5 further comprising adjuvants, solvents, carrier, surfactants or extenders.

9. Use of an active compound combination according to any of claims 1 to 6 for the treatment of seed.

10. Seed that has been treated with an active compound combination according to any of claims 1 to 6.

11. A method for curative ly or preventively controlling the phytopathogenic fungi of plants or crops comprising the use of an active compound combination according to any of claims 1 to 6 or a composition according to claim 7 or 8 for control of powdery mildew diseases.

12. The method according to claim 11 characterized in that an active compound combination to any of claims 1 to 6 or a composition according to claim 7or 8 is applied to the seed, the plant, to fruits of plants or to the soil on which the plant grows or is supposed to grow.

13. Use of an active compound combination according to any of claims 1 to 6 or a composition according to claim 7 or 8 for treatment of diseases caused by powdery mildew pathogens.

14. Use according to claim 13 characterized in that an active compound combination according to any of claims 1 to 6 or a composition according to claim 7 or 8 is applied to the seed, the plant, to fruits of plants or to the soil on which the plant grows or is supposed to grow.