WO2015116907A1 - Aipa, ompa, and asp14 in vaccine compositions and diagnostic targets for anaplasma phagocytophilum infection - Google Patents

Aipa, ompa, and asp14 in vaccine compositions and diagnostic targets for anaplasma phagocytophilum infection Download PDF

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WO2015116907A1
WO2015116907A1 PCT/US2015/013715 US2015013715W WO2015116907A1 WO 2015116907 A1 WO2015116907 A1 WO 2015116907A1 US 2015013715 W US2015013715 W US 2015013715W WO 2015116907 A1 WO2015116907 A1 WO 2015116907A1
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ompa
seq
aipa
phagocytophilum
cells
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PCT/US2015/013715
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French (fr)
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Jason CARLYON
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Virginia Commonwealth University
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Priority to EP15742728.7A priority Critical patent/EP3102234B1/en
Priority to US15/115,954 priority patent/US10086058B2/en
Publication of WO2015116907A1 publication Critical patent/WO2015116907A1/en
Priority to US16/105,048 priority patent/US10842860B2/en
Priority to US17/071,385 priority patent/US11458197B2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/0233—Rickettsiales, e.g. Anaplasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/29—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Richettsiales (O)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention generally relates to a vaccine or immunogen and diagnostic for anaplasmosis in mammals.
  • the invention provides Anaplasma phagocytophilum (AipA) protein and fragments thereof comprising an invasin domain for these purposes, used alone or in combination with one or both of Anaplasma phagocytophilum outer surface protein A (OmpA) and/or antigenic fragments thereof, and Anaplasma phagocytophilum surface protein 14 (Asp 14) and/or antigenic fragments thereof.
  • 02940981TA_ST25.txt is 108 kilobytes, and was created on January 30, 2015.
  • Anaplasma phagocytophilum ⁇ Aph is a tick-transmitted obligate intracellular bacterium of the family Anap I as mataceae that can infect humans, livestock, companion animals, and wild animals.
  • the Anaplasmataceae family members include Anaplasma marginale, Anaplasma platys, Ehrlichia chaffeensis, Ehrlichia canis, and Ehrlichia ruminatium, among others, and all of these cause similar infections known collectively as ehrlichiosis or anaplasmosis.
  • HGA human granulocytic anaplasmosis
  • Other possible disease manifestations include leukopenia, thrombocytopenia, and elevated serum transaminase levels. Unfortunately, no vaccines against these infectious agents are currently available.
  • antibiotic treatments with doxycycline or tetracycline are currently the frontline treatments for anaplasmosis/ehrlichiosis and can be effective.
  • the overuse of antibiotics is currently of great concern, and many individuals, such as those that are allergic to the drug, pregnant women, and small children, either cannot or are not advised to take doxcycline. Also troubling is the lack of tools for rapid and definitive diagnosis of
  • HGA is an emerging and potentially fatal disease transmitted by the same vectors that transmit Lyme Disease, primarily ticks and deer, but other animal and human hosts can complete the vector cycle and therefore extend the spread of disease.
  • Lyme Disease primarily ticks and deer
  • HGA occurs primarily in the Northeast, Upper Midwest, and Northern California. Since HGA became a reportable disease in the U.S. in 1999, the number of cases has risen annually, reaching 2,037 in 2013. Since diagnostic tools for HGA are lacking, the number of actual cases is likely to be much higher.
  • HGA is increasingly recognized in Europe and Asia, and Aph infection is now the most widespread tick-transmitted disease of animals in Europe. Domestic animals, such as dogs and cats, and livestock, such as sheep can be become infected.
  • an obligate intracellular bacterium must enter a target cell in its human or animal host to survive, replicate, and move to the next host.
  • an Anaplasma spp. or Ehrlichia spp. infected tick bites a subject the bacteria is transferred from the tick salivary glands into the tissues of the host or into the bloodstream where they bind to the surface of host cells (especially neutrophils) and are internalized by being taken up into vacuoles that form around each bacterium.
  • host cells especially neutrophils
  • A. phagocytophilum optimal invasion of mammalian host cells is known to involve multiple cell surface proteins (various adhesins and invasins) which function cooperatively.
  • a resident bacterium prevents the vacuole from merging with lysosomes, which would otherwise destroy the bacterium. In doing so, the bacterium converts the cell into a protective niche that favors bacterial survival and completion of its zoonotic cycle.
  • A. phagocytophilum undergoes a biphasic developmental cycle within neutrophils in which it transitions between an infectious morphotype (dense-cored cell; DC) and a noninfectious, replicative morphotype (reticulate cell; RC).
  • Aph infection is Aph colonization of neutrophils
  • Aph has also been detected in the microvascular endothelium of heart and liver in experimentally infected severe combined immunodeficiency mice.
  • promyelocytic and endothelial cell lines are also useful as in vitro models for studying Aph-host cell interactions.
  • Bovine anaplasmosis causes severe economic loss in many countries, mainly due to the high morbidity and mortality in susceptible cattle herds.
  • the losses incurred by the U.S. cattle industry as a result of anaplasmosis are estimated to be $300 million per year whereas in Latin America, losses are calculated to be approximately $800 million per year.
  • Proteins which mediate the infection of endothelial cells and/or the dissemination of bacteria into the microvasculature of heart and liver are attractive targets for preventing disease caused by Anaplasmataceae bacteria such as Aph.
  • targeting ⁇ p/z proteins that are conserved among related Anaplasmataceae family members may also reduce or block
  • IgM antibodies which usually rise at the same time as IgG near the end of the first week of illness and remain elevated for months or longer, are even less specific than IgG antibodies and more likely to result in a false
  • EIA tests are qualitative rather than quantitative, meaning they only provide a positive/negative result, and are less useful to measure changes in antibody titers between paired specimens.
  • EIA assays rely on the evaluation of IgM antibody alone, which again may have a higher frequency of false positive results.
  • PCT/US2013/047325 to Carlyon which is herein incorporated by reference, relates to the invasin proteins OmpA and Asp 14, and fragments thereof comprising conserved invasin domains.
  • the proteins and fragments are used as therapeutic and diagnostic agents for phagocytophilum infection.
  • Anaplasmataceae invasin proteins provides a path forward for the development of therapies to treat and/or prevent Anaplasmataceae infections.
  • the discovery and characterization of such invasins also leads to improved diagnostic methods for detecting exposure to and/or infection with Anaplasmataceae, so that proper therapeutic measures can be undertaken.
  • AipA Anaplasma phagocytophilum invasion protein A
  • This protein corresponds to protein APH0915 identified by Nelson et al. (2008).
  • the studies presented in Example 1 below demonstrate that AipA is in fact an outer membrane protein, and further that AipA is, surprisingly, an A. phagocytophilum invasin protein that is useful as a therapeutic and diagnostic agent.
  • AipA is unique to A. phagocytophilum i.e. homologs of AipA are not present in other Anaplasmataceae species. Accordingly, certain aspects of this disclosure provide AipA and/or one or more functional fragments thereof for use as components of vaccines and/or immunogenic compositions to prevent or treat A. phagocytophilum infections.
  • Exemplary functional domains of AipA include but are not limited to fragments which include a linear amino acid sequence encompassing amino acids 1 -87 inclusive, and amino acids 9-21 (SEQ ID NO: 13), inclusive.
  • AipA and/or one or more of the identified functional fragments thereof are used for these purposes in combination with one or more additional invasins and/or functional domains of the additional invasins.
  • additional invasins include but are not limited to Aspl4 (14-kDa phagocytophilum surface protein; APH0248) and OmpA (Outer membrane protein A; APH0338 in the annotated A. phagocytophilum proteome), and homologs thereof from other species, e.g. other Anaplasmataceae species.
  • Exemplary functional domains of Asp 14 include but are not limited to fragments which include the linear amino acid sequence from amino acids: 19-60, 101-1 12, 101-124, and 1 13-124, all of which are inclusive.
  • the sequence is that which encompasses amino acids 1 13-124.
  • Exemplary functional domains of OmpA include but are not limited to fragments which include the amino acid sequence from amino acids: 19-74, 59-74, 48-56, 86-101 , 135-155, and 24-121 , all of which are inclusive.
  • the sequence is that which encompasses amino acids 59-74.
  • phagocytophilum surface proteins and/or invasion domain-containing fragments thereof as well as antibodies to one or more of these proteins or fragments, as protective/therapeutic/diagnostic agents or targets to treat or detect Anaplasmataceae infection.
  • some aspects of the invention provide therapeutic agents which are or which are based on combinations of at least two and usually all three of the proteins or relevant fragments thereof.
  • therapeutic agents which are or which are based on combinations of at least two and usually all three of the proteins or relevant fragments thereof.
  • linear peptide domains of each protein that mediate invasin and combinations of linear peptide domains are encompassed, as are antibodies targeting one, two, or all three domains. Such agents are shown herein to simultaneously work synergistically to nearly abolish A.
  • phagocytophilum invasion of host cells For example, antisera combinations targeting two of the three invasins together more effectively inhibited infection than an antiserum targeting an individual protein, and the most effective blocking of A. phagocytophilum infection was achieved using antisera against all three invasins.
  • An embodiment of the invention is a composition comprising one or more polypeptides which include one copy or more than one copy of SEQ ID NO: 14 together with one copy or more than one copy of either or both of SEQ ID NO: 3 and SEQ ID NO: 06.
  • a single polypeptide might include 1 , 2, or 3 copies of SEQ ID NO: 14 together with 0, 1, 2 or 3 copies of SEQ ID NO. 3 and/or 0, 1 , 2 or 3 copies of SEQ ID NO. 6, and may include additional amino acid sequences of interest (e.g. linkers, etc.).
  • the composition may include two or more polypeptides wherein one polypeptide is or includes one or more copies of SEQ ID No. 14, and the other polypeptide(s) is/are or include(s) one or more copies of either or both of SEQ ID No. 3 and SEQ ID No. 6.
  • the isolated peptides are in a vehicle or carrier suitable for administration to a subject.
  • At least one of the polypeptides may be linked to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, or one or more additional polypeptides comprising SEQ ID NO:01 , 02, 03, 04, 05, 06, 13, 14, or 15 or a combination thereof.
  • An exemplary embodiment is one or more polypeptides linked to a protein purification ligand, and protein purification ligands are a peptide encoding a histidine tag.
  • compositions that can be used for generating an immune response.
  • the compositions may take the form of DNA vaccines or other nucleic acid vaccines which comprise nucleic acids encoding one or more polypeptides as described herein, e.g. polypeptides which include one or more copies of SEQ ID NO: 14, optionally together with one or more copies of either or both SEQ ID NO: 3 and SEQ ID NO. 06.
  • a suitable composition might include a single nucleic acid in a vector (e.g., adenovirus, baculovirus, etc.) that codes for a single polypeptide or multiple polypeptides that include 1 , 2, or 3 copies of SEQ ID NO: 14, together with 0, 1, 2 or 3 copies of SEQ ID NO. 3; and 0, 1, 2 or 3 copies of SEQ ID NO. 6, and may include additional amino acid sequences of interest, linkers, etc.
  • the composition may include two or more nucleic acids, each on a vector, which code for a polypeptide that is or includes one or more repeats of SEQ ID NO. 14, and code for another polypeptide is or includes one or more repeats of either or both SEQ ID NO. 3 and SEQ ID NO.
  • DNA may also be provided without a vector and be combined with material which might also be driven into a cell or living tissue by an agent which crosses into a cell (e.g., Lipofectamine).
  • the DNA vaccines might be incorporated into a host bacterium that is itself administered as a vaccine to a subject.
  • Another embodiment of the invention is a method of protecting a subject from acquiring a zoonotic disease and/or treating a zoonotic disease in a subject by the step of administering a composition an immunogenic composition comprising the isolated polypeptide represented by SEQ ID NO: 14 and at least one of the isolated polypeptides represented by SEQ ID NO:03 and SEQ ID NO:06.
  • At least one of the polypeptides may be linked to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, or one or more additional polypeptides comprising SEQ ID NO:01, 02, 03, 04, 05, 06, 13, 14, or 15; or a combination thereof.
  • the zoonotic disease may be one caused by an obligate intracellular Anaplasmataceae bacterium selected from the group consisting of Anaplasma phagocytophilum, Anaplasma marginale,
  • the zoonotic disease may be human granulocytic anaplasmosis (HGA) if caused by Anaplasma phagocytophilum.
  • HGA human granulocytic anaplasmosis
  • the subject is an animal, the zoonotic disease may be anaplasmosis. If caused by an ehrlichia species, the human zoonotice disease may be monocytic ehrlichiosis or ehrlichiosis.
  • Another embodiment of the invention is a method of detecting antibodies that specifically bind an Anaplasmataceae polypeptide in a test sample.
  • the method may include the steps of contacting a test sample, under conditions that allow polypeptide-antibody complexes to form, with a composition that includes at least one or more polypeptides encoding all or a portion of SEQ ID NO:01, SEQ ID NO:04, and/or SEQ ID NO: 13, and detecting said polypeptide-antibody complexes, wherein the detection is an indication that antibodies specific for Anaplasmataceae Asp 14, OmpA, or AipA are present in the test sample.
  • antibodies are generated to at least one or more polypeptides encoding all or a portion of SEQ ID NO:01, SEQ ID NO:04, and/or SEQ ID NO:13, and the antibodies are used to detect the presence of AipA and/or OmpA and/or Asp 14 protein in a biological sample from a subject.
  • the method may be an assay selected from the group consisting of an immunoblot and an enzyme-linked immunosorbent assay (ELISA).
  • An exemplary embodiment of this methodology includes using at least one polypeptide which is or includes SEQ ID NO:03, SEQ ID NO:06, or SEQ ID NO: 14 in the assay, whereby infection with obligate intracellular Anaplasmataceae is determined from a serum sample exhibiting antibody binding with the at least one polypeptide.
  • FIG. 1 A-B Schematic diagrams of A. phagocytophilum AipA membrane topology and sequence.
  • A Diagram of the predicted topology of AipA in the A, phagocytophilum outer membrane.
  • N AipA amino terminus.
  • C AipA carboxy terminus. Numerical values indicate amino acid coordinates for predicted transmembrane spanning regions.
  • B Diagrams of the AipA sequence. The scale indicates 50-amino acid intervals.
  • the Kyte-Doolitle algorithm was used to determine hydrophobic (histogram above the axis) and hydrophilic (histogram below axis) regions.
  • the Emini algorithm was used to determine regions that are likely accessible on the surface of the AipA (histogram above the axis) or not (histogram below the axis).
  • the AipA amino acid segments against which antisera were raised are indicated on the Hydrophobicity plot by horizontal lines.
  • Figure 2 A-F Differential expression profiling of AipA throughout the A.
  • phagocytophilum life cycle (A) aipA transcriptional profile during A. phagocytophilum infection of HL-60 cells. DC bacteria were incubated with HL-60 cells to establish a
  • aipA transcript levels were normalized to A. phagocytophilum 16s rRNA gene transcript levels.
  • normalized aipA transcript levels per time point were calculated as the fold change in expression relative to expression at 16 h, a time point at which the entire bacterial population is in the DC morphotype. Data are the means + standard deviations (SD) for triplicate samples and are representative of two experiments having similar results.
  • phagocytophilum infected ticks on naive mice A. phagocytophilum-mfected I. scapularis nymphs were allowed to feed on mice for 72 h. Total RNA recovered from unfed and transmission-fed ticks that had been removed at 24, 48, and 72 h postattachment was subjected to RT-qPCR. Relative aipA and groEL transcript levels were normalized to A. phagocytophilum 16S rRNA gene levels.
  • AipA is not expressed during A. phagocytophilum infection of a tick cell line. Western blot analysis of uninfected and A.
  • phagocytophilum infected HL-60 and ISE6 cells using antiserum specific for AipAi -8 7 or APH0032. The number of weeks (Wk.) during which A. phagocytophilum was maintained in ISE6 cells are indicated.
  • AipA is expressed in vivo and elicits a humoral immune response. Western blot analysis of GST and GST-AipAi-87 screened with sera from an HGA patient and from an A. phagocytophilum infected dog. Results presented in panels B to F are each representative of at two to three independent experiments with similar results. Statistically significant (*, P ⁇ 0.05; **, P ⁇ 0.005; ***, P ⁇ 0.001) values are indicated.
  • AipA is an A. phagocytophilum OMP.
  • AipA colocalizes with the confirmed OMP, Msp2 (P44).
  • A. phagocytophilum-infected RF/6A cells were fixed and viewed by confocal microscopy to assess immunoreactivity with AipA antiserum in conjunction with Msp2 (P44) antiserum.
  • Host cell nuclei were stained with DAPI (4',6'-diamidino-2- phenylindole). The insets demarcated by solid boxes in the lower right corners of each panel are magnified versions of the representative A. phagocytophil m-occwpied vacuole that is denoted by the hatched box in each panel.
  • B AipA is exposed on the bacterial surface. Intact A.
  • phagocytophilum DC organisms were incubated with trypsin or vehicle control, solubilized, and Western-blotted. Immunoblots were screened with antiserum targeting AipAi -87 , Asp55, or APH0032. Data are representative of two experiments with similar results.
  • FIG. 4A-F GST-AipA requires amino acids 1 to 87 to bind and competitively inhibit phagocytophilum infection of mammalian host cells.
  • a and B GST-AipAi -8 7 binds to mammalian host cells. RF/6A cells were incubated with GST-AipAi -87 , GST-AipA 2 49- 3 55, or GST alone.
  • A The host cells were fixed, screened with GST antibody, and examined using confocal microscopy. Host cell nuclei were stained with DAPI. Representative merged fluorescent images from three experiments with similar results are shown.
  • B Flow cytometric analysis of GST fusion protein binding to RF/6A cells.
  • Figure 5A-C Pretreatment of A. phagocytophilum with AipAi-8 7 antiserum inhibits infection of HL-60 cells but does not alter binding to sLe x -capped PSGL-1.
  • phagocytophilum DC organisms were exposed to antiserum targeting AipAi -87 , AipA 2 49 -35 5, OmpA, or preimmune serum and then incubated with HL-60 (A and B), PSGL-1 CHO cells, or untransfected CHO cells (C).
  • HL-60 A and B
  • PSGL-1 CHO cells PSGL-1 CHO cells
  • C untransfected CHO cells
  • the infection of HL-60 cells was allowed to proceed for 24 h prior to being assessed, while bacterial binding to PSGL-1 CHO cells was assessed immediately.
  • phagocytophilum (Ap) vacuolar inclusions per HL-60 cell (B), and bound DC organisms per PSGL-1 CHO cell or untransfected CHO cell (C) were determined using immunofluorescence microscopy. Additional positive controls for blocking ⁇ , phagocytophilum to PSGL-1 CHO cells, besides incubating bacteria with OmpA antiserum, were PSGL-1 CHO cells that had been incubated with PSGL-1 N-terminus blocking antibody KPL-1 or sLe x -blocking antibody CSLEXl prior to the addition of bacteria. Negative controls were PGSL-1 CHO cells that had been incubated with isotype control antibodies prior to the addition of bacteria. Results shown are relative to GST-treated host cells and are the means + SD for three experiments. Statistically significant (***, P ⁇ 0.001) values are indicated.
  • FIG. 6A-B A combination of antisera targeting AipA, OmpA, and Aspl4 blocks A. phagocytophilum infection of host cells. DC bacteria were incubated with preimmune serum or antiserum targeting AipAi -87 , OmpA, and/or Aspl4 and then incubated with HL-60 cells.
  • A The cells were fixed and screened by confocal microscopy to assess the percentage of infected cells. Results shown are relative to host cells that had been treated with preimmune serum and are the means + SD for three experiments.
  • phagocytophilum 16s rRNA gene were normalized to DNA loads of the human ⁇ -actin gene. Results shown are the means + SD of triplicate samples and are representative of three independent experiments with similar results. Statistically significant (*, P ⁇ 0.05; **, P ⁇ 0.005; ***, P ⁇ 0.001) values relative to the bacterial load of host cells that had been incubated with preimmune antisera are presented.
  • FIG. 7A-C AipA residues 9-21 are critical for establishing infection in host cells.
  • A Western blot analyses in which rabbit antiserum targeting AipA ⁇ i, AipA6i -8 4, AipAi 65- i 82 , AipAi 83-201 , AipAi -87 , or preimmune rabbit serum was used to screen whole cell lysates of uninfected (U) and A. phagocytophilum infected HL-60 cells (I). Data are representative of two experiments with similar results.
  • DC bacteria were pretreated with antiserum specific for AipA 9-2 i , AipA 6 i -84 , AipAi 65- i 82 , AipAi 83-2 oi, AipAi -87 , AipA 24 9-3 55 , OmpA, or preimmune serum for 30 min.
  • the treated bacteria were incubated with HL-60 cells for 60 min.
  • host cells were incubated for 24 h and subsequently examined using Msp2 (P44) antibody and confocal microscopy to assess the percentage of infected cells. Results shown are relative to preimmune serum-treated host cells and are the means + SD for six experiments.
  • Statistically significant (*, P ⁇ 0.05; **, P ⁇ 0.005; ***, P ⁇ 0.001) values are indicated.
  • FIG. 8A-C OmpA amino acids 59 to 74 are critical for A. phagocytophilum to bind to sLe x -capped PSGL-1 and for infection of mammalian host cells.
  • A ELISA in which OmpA23-40, OmpA41 -58, and OmpA59-74 antibodies (diluted 1 : 1600) were used to screen wells coated with GST, GST-OmpA, GST-OmpA 19-74, GST-OmpA75-205, or peptides corresponding to OmpA23-40, OmpA41-58, or OmpA59-74. Results shown are the mean + SD of triplicate samples and are representative of three independent experiments with similar results.
  • B Pretreatment of A.
  • phagocytophilum with OmpA59-74 antibody inhibits infection of HL-60 cells in a dose-dependent manner.
  • DC bacteria were incubated with 200 ⁇ g/ml of preimmune serum, 200 ⁇ ig/ml of serum raised against GST-OmpA, or twofold serially-diluted concentrations of sera raised against OmpA23-40, OmpA41-58, or OmpA59-74 ranging from 0 to 200 ⁇ g/ml and then incubated with HL-60 cells. The infection was allowed to proceed for 24 h after which the cells were fixed and examined using immunofluorescence microscopy to quantify the percentage of infected cells.
  • Results shown are relative to host cells that had been incubated with bacteria exposed to preimmune serum and are representative of three experiments with similar results.
  • C OmpA59-74 antibody inhibits A. phagocytophilum binding to sLe x -capped PSGL-1.
  • DC bacteria were exposed to preimmune serum, antibodies against OmpA, OmpA23-40, OmpA41-58, or OmpA59-74 and then incubated with PSGL-1 CHO cells.
  • Bacteria that were not exposed to antibodies and incubated with PSGL-1 CHO cells or CHO (-) cells were positive and negative controls, respectively, for bacterial binding.
  • the mean numbers + SD of bound DC organisms per cell were determined using immunofluorescence microscopy. Results shown are the mean + SD of six combined experiments. Statistically significant (** P ⁇ 0.005; ***P ⁇
  • FIG. 9A-F Molecular docking models of A. phagocytophilum OmpA-sLe interactions.
  • A Predicted tertiary structure for A. phagocytophilum OmpA. The dotted line separates the regions encompassed by residues 19 to 74 and 75 to 205. Residues K60, G61, and K64 positions are indicated.
  • B Stick representation of the N-terminal PSGL-1 amino acids 61 to 77 capped with sLe x derived from PDB lgls. The sLe x glycan extends off of threonine 73. sLe x linkages and individual sugar residues are denoted.
  • C Electrostatic surface map of A.
  • phagocytophilum OmpA as generated using the PyMol APBS plugin.
  • the image is oriented as in (A).
  • OmpA is presented as a ribbon model, sLe x as a stick model, and hydrogen bonding by dotted lines.
  • OmpA residue 64 is predicted to interact with a2, 3-1080 sialic acid of sLe x (E and F).
  • Residue G61 is predicted to interact with either a2,3-sialic acid (E) or al ,3-fucose of sLe (F).
  • Residue 60 is predicted to interact with ⁇ 1 ,3 galactose of sLe x (F).
  • FIG. 10A-B OmpA is highly conserved among A. phagocytophilum isolates and its key binding residues exhibit variable conservation among Anaplasmataceae species.
  • A Alignment of OmpA amino acid sequence from the A. phagocytophilum strain (isolated from a human patient in Massachusetts), with OmpA sequences from A. phagocytophilum strains HZ (human; New York), HGE1 (human; Minnesota), Dog (Minnesota), JM (jumping mouse;
  • the binding domain corresponding to NCH-1 OmpA comprises residues 59 to 74. Numbers above the alignments in (A) and (B) denote amino acid position numbers. The arrows in (A) and (B) denote d.
  • phagocytophilum OmpA G61and K64 which were predicted to form interactions with sLe in Figure 9 panels E and F and were shown to be critical for OmpA to bind to and mediate infection of mammaliam host cells in Figures 8 and 1 1.
  • FIG. 11A-D G61 and K64 are essential for recombinant OmpA to optimally bind to mammalian host cells and competitively inhibit s, phagocytophilum infection.
  • GST1087 OmpA proteins having the CLNHL peptide inserted between OmpA amino acids 67 and 68 or having G61 and/or K64 mutated to alanine are unable to bind to competitively inhibit A.
  • HL-60 cells were incubated with DC organisms in the presence of GST alone, GST-OmpA, GST-OmpA proteins bearing insertions of CLNHL (SEQ ID NO:75) between the indicated residues (A), or GST-OmpA proteins having the indicated amino acids substituted with alanine (B and C) for 1 h. After washing to remove unbound bacteria, host cells were incubated for 24 h and subsequently examined by
  • Figure 12A-C Treatment with al,3/4-fucosidase reduces A. phagocytophilum binding to PSGL-1 CHO cells and binding to and infection of RF/6A endothelial cells.
  • PSGL-1 CHO cells (A) and RF/6A cells (B and C) were treated with al ,3/4-fucosidase (+ fucosidase) or vehicle control (- fucosidase). Fucosidase- and mock-treated cells were incubated with A. phagocytophilum DC organisms. Following the removal of unbound bacteria, the infection of RF/6A cells was allowed to proceed for 24 h prior to being assessed, while bacterial binding to PSGL-1 CHO and RF/6A cells was examined immediately. The mean + SD of bound DC bacteria per PSGL-1 CHO (A) or RF/6A cell (B) or percentage of infected RF/6A cells (C) were determined using immunofluorescence microscopy. Results shown are the means + SD for three combined experiments. Statistically significant (***P ⁇ 0.001) values are indicated.
  • FIG. 13A-E OmpA interacts with l,3-fucose on mammalian host cell surfaces.
  • a to D RF/6A cells were treated with al ,3/4-fucosidase (A and C), a2,3/6-sialidase (B and D), or vehicle control (- fucosidase and - sialidase, respectively).
  • Glycosidase- and mock-treated cells were incubated with al,3/6-fucose-specific lectin, AAL; the a2,3- sialic acid-specific lectin, MAL II; His-OmpA; or His-Aspl4.
  • A The host cells were fixed and screened using
  • a and B immmunofluorescence microscopy
  • C and D flow cytometry
  • E AAL and MAL II competitively inhibit His- OmpA binding to mammalian host cells.
  • RF/6A cells were incubated with AAL and MAL II, after which His-OmpA was added. Following the removal of unbound recombinant protein, His- OmpA bound on RF/6A cell surfaces was detected by flow cytometry.
  • Statistically significant ***P ⁇ 0.001) values are indicated. Results shown are representative of three experiments with similar results.
  • FIG. 14A-D OmpA interacts with 6-sulfo sLe* on RF/6A endothelial cell surfaces.
  • FIG. 1 Schematic representations of sLe x and 6-sulfo sLe x . Individual sugar and glycosidic linkages are indicated.
  • B and C 6-sulfo sLe x is present in high abundance relative to sLe x on RF/6A cells.
  • RF/6A cells were screened with sLe -specific antibodies, CSLEX1 and KM93; 6-sulfo sLe x -specific antibody, G72; or IgM isotype control followed by detection of cell surface bound antibodies using immunofluorescence microscopy (B) and flow cytometry (C).
  • D Antibody blocking of 6-sulfo sLe" inhibits His-OmpA binding to RF/6A cells.
  • RF/6A cells were incubated with CSLEXl , KM93, G72, IgM, or vehicle (Cells only) followed by the addition of His-OmpA, and washing to remove unbound recombinant protein. Flow cytometry was used to detect bound His-OmpA. Statistically significant (*** p ⁇ 0.001) values are indicated. Results shown are representative of two experiments with similar results.
  • FIG. 15A-D OmpA coated beads bind to and are internalized by non-phagocytic endothelial cells.
  • OmpA-coated microspheres were incubated with OmpA antibody (aOmpA) or isotype control. Unconjugated (Control) beads were included as a negative control. Bound antibody was detected using immunofluorescence microscopy.
  • B to D OmpA coated beads bind to and are internalized by nonphagocytic RF/6A cells. OmpA coated beads were incubated with RF/6A cells for 1 h after which unbound beads were washed off. Cells were screened with OmpA antibody and examined using immunofluorescence (B and D) or scanning electron microscopy (C) to assess binding or were incubated further to allow for bead uptake.
  • FIG 16A-I OmpA coated bead binding to and uptake by promyelocytic HL-60 cells involve OmpA residues G61 and K64 and are dependent on sLe ⁇
  • A Scanning electron micrographs depicting OmpA coated beads bound to and being internalized by HL-60 cells. Arrows point to filopodia-like structures adhered to beads. Scale bars are indicated.
  • B and C HL-60 cells were incubated with beads coated with OmpA, OmpA proteins having the indicated amino acids substituted with alanine, or non-coated control beads. The numbers of bound and internalized beads were determined using immunofluorescence microscopy.
  • FIG. 17A-D The Aspl4 binding domain is contained within amino acids 113 to 124.
  • A. phagocytophilum Pretreatment of A. phagocytophilum with Aspl41 13-124 antiserum inhibits infection of HL-60 cells in a dose-dependent manner.
  • DC bacteria were incubated with 200 ng/ml of preimmune serum, 200 ⁇ g/ml of serum raised against full-length Asp 14, or two-fold serially- diluted concentrations of anti -Asp 1498-112 or anti-Asp 14113-124 ranging from 0 to 200 ⁇ g/ml and then incubated with HL-60 cells. The infection was allowed to proceed for 24 h prior to being assessed by immunofluorescence microscopy for the percentage of infected cells.
  • DC organisms were exposed to preimmune serum or antisera targeting OmpA59-74, Aspl41 13-124, OmpA59- 74 plus Aspl498-1 12, or anti- Aspl41 13-124 together with OmpA59-74, OmpA23-40, or OmpA43-58 antibodies. The cells were fixed and screened using immunofluorescence microscopy to determine the percentages of infected cells.
  • C and D OmpA59-74 and
  • Aspl 4113-124 Fab fragments effectively inhibit A. phagocytophilum infection of HL-60 cells.
  • DC bacteria were incubated with Fab fragments derived from preimmune serum, antibodies targeting OmpA23-40, OmpA41-58, OmpA59-74, Aspl498-1 12, Aspl4113-124, or OmpA59-74 Fab fragment together with Aspl4113- 124 Fab fragment.
  • the cells were fixed and screened to determine the percentages of infected cells (C) and morulae per cell (D).
  • Results presented in (B) to (D) are relative to host cells that had been incubated with bacteria treated with preimmune serum.
  • Results presented in (A) and (B) are the means + SD for three experiments.
  • Results in (C) and (D) are the mean + SD of triplicate samples and are representative of two experiments with similar results.
  • Statistically significant (* P ⁇ 0.05; ** P ⁇ 0.005; ***P ⁇
  • FIG. 18A-C A combination of antisera targeting the binding domains of OmpA,
  • AipA and suitable fragments thereof which represent domains involved in bacterial invasion, can be used as both therapeutic agents (e.g. immunogenic agents and vaccinogens to prevent or treat infection); as diagnostic agents (e.g. as targets to detect antibodies to A. phagocytophilum in biological samples); and/or to generate antibodies which are used as therapeutic agents or as diagnostic agents to detect A. phagocytophilum AipA protein in biological samples.
  • therapeutic agents e.g. immunogenic agents and vaccinogens to prevent or treat infection
  • diagnostic agents e.g. as targets to detect antibodies to A. phagocytophilum in biological samples
  • diagnostic agents e.g. as targets to detect antibodies to A. phagocytophilum in biological samples
  • AipA and/or suitable effective fragments thereof are used for these purposes in combination with one or more other invasin proteins or suitable effective invasin fragments thereof, examples of which include OmpA and Asp 14. Homologs of OmpA and Asp 14 may also be employed for these purposes.
  • the critical regions of OmpA and Asp 14 that mediate infection are highly conserved among family members Aph, A. marginale, and closely related Ehrlichia species, such as E. chaffeensis, E. canis, and E. ruminatium, and may be highly conserved in A. platys.
  • Aph and A. marginale are closely related and express many gene homologs, including Asp 14 and OmpA and other surface antigens.
  • AipA has no known homologs in other Anaplasma and Ehrlichia species (all of which cause disease in humans and/or animals - cattle, horses, sheep, dogs, cats) or other non-Anaplasmataceae organisms.
  • AipA is unique to A. phagocytophilum and is thus not employed alone to treat or diagnose infections other than A. phagocytophilum.
  • assays and methods of using the assays to distinguish exposure to or infection with A. phagocytophilum from exposure to or infection with a non-A phagocytophilum Anaplasmataseae species are provided, e.g. to permit specialized treatment and/or to track the occurrence or frequency of A. phagocytophilum infections.
  • a biological sample from a subject is thus screened with two or more of the agents described herein, at least one or which is AipA based or derived, and if a sample contains (is positive for the presence of) AipA proteins or antibodies to AipA proteins, it is concluded that the subject is infected with (or at least was previously exposed to) A. phagocytophilum. Previous exposure to A.
  • phagocytophilum may result from a prior vaccination. Therefore, the methods of the invention may be used to determine if a subject has or has not been previously vaccinated. However, if the sample contains only OmpA and/or Asp 14 proteins or antibodies, but AipA proteins/antibodies are absent, a conclusion is drawn that the subject is infected or has been exposed to an Anaplasmataseae species that is not A. phagocytophilum. If the subject is currently infected with A.
  • phagocytophilum then treatment specific for that organism may be undertaken, e.g.
  • compositions comprising AipA protein or fragments thereof as described herein
  • administration of antibodies directed specifically to AipA protein or fragments thereof etc.
  • Anaplasma phagocytophilum or A. phagocytophilum, an Anaplasmataseae family bacterium that is tick-born and causes anaplasmosis in humans and animals.
  • Anaplasmataceae a family of closely related bacteria, including Anaplasma and Ehrlichia species. The genera Neorickettsia and Wolbachhia are also Anaplasmataceae, bacteria but do not cause anaplasmosis.
  • Antigen term used historically to designate an entity that is bound by an antibody, and also to designate the entity that induces the production of the antibody. More current usage limits the meaning of antigen to that entity bound by an antibody, while the word "immunogen" is used for the entity that induces antibody production. Where an entity discussed herein is both
  • an antigen, immunogen or epitope is generally a portion of a protein (e.g. a peptide or polypeptide).
  • AipA 36.9-kilodalton ⁇ ? ! surface protein. AipA has no known homologs in other Anaplasma and Ehrlichia species or other non-Anaplasmataceae organisms.
  • Aspl4 14-kilodalton ⁇ /z surface protein. Aspl4 homologs are expressed by Anaplasmataceae family members, including Aph, A. marginale, Ehrlichia chaffeensis, E. canis, E. ewingii, and E. ruminatium.
  • OmpA Outer membrane protein A. OmpA homologs are expressed by Anaplasmataceae family members, including Aph, A. marginale, Ehrlichia chaffeensis, E. canis, E. ewingii, and E.
  • DC and RC Aph undergoes a biphasic developmental cycle, the kinetics of which have been tracked in promyelocytic HL-60 cells. The cycle begins with attachment and entry of an infectious dense-cored (DC) organism. Once intracellular, the DC differentiates to the noninfectious reticulate cell (RC) form and replicates by binary fission to produce a bacteria- filled organelle called a morula. Later, the RCs transition back to DCs, which initiate the next round of infection.
  • DC dense-cored
  • Epitope a specific chemical domain on an antigen that is recognized by a B-cell receptor, and which can be bound by secreted antibody.
  • the term as used herein is interchangeable with "antigenic determinant”.
  • An epitope may comprise a single, non-interrupted, contiguous chain of amino acids joined together by peptide bonds to form a peptide or polypeptide. Such an epitope can be described by its primary structure, i.e. the linear sequence of amino acids in the peptide chain.
  • Epitope may also refer to conformational epitopes, which are comprised of at least some amino acids that are not part of an uninterrupted, linear sequence of amino acids, but which are brought into proximity to other residues in the epitope by secondary, tertiary and/or quaternary interactions of the protein. Residues in conformational epitopes may be located far from other residues in the epitope with respect to primary sequence, but may be spatially located near other residues in the conformational epitope due to protein folding.
  • Immunodominant epitope The epitope on a molecule that induces the dominant, or most intense, immune response.
  • the immunodominant epitope would elicit the greatest antibody titer during infection or immunization, as measured by, for example, the fraction of reactivity attributable to a certain antigen or epitope in an enzyme-linked immunosorbant assay as compared with the total responsiveness to an antigen set or entire protein.
  • An invasin domain is a region of a pathogen's protein that binds a host cell and mediates intracellular signaling and pathogen entry into the host cell. In some cases, uptake of the pathogen results in the formation of a vacuole in which the intracellular pathogen will reside.
  • the invasin domains of the invention are linear amino acid sequences within Asp 14, OmpA, AipA or other surface proteins that are found on the outer membrane of the bacteria Aph and other Anaplasmataceae family members, and can vary slightly from one family member to the next. However, the invasin domain in each Asp 14 homolog is critical for uptake of bacteria into host cells (known to be neutrophils and endothelial cells in the case of Anaplasmataceae).
  • Invasin domains may be referred to herein as "effector domains”.
  • linear amino acid sequence, inclusive refers to a sequence that includes the first- and last-numbered amino acids, e.g. "amino acids 1-87 inclusive” refers to a linear amino acid sequence that includes amino acids 1 , 2, 3... etc. up to and including amino acids ...85, 86 and 87 of a sequence, e.g. a sequence specified as a SEQ ID NO: or in referenced to a SEQ ID NO:.
  • Linker sequences short peptide sequences encoding functional units that may be engineered or otherwise added at the ends or within recombinant proteins, polypeptides, peptides of interest. Linker sequences may be used as "handles" for protein purification, as detectable signals of expression or binding to other proteins or macromolecules, to modulate tertiary structure, or enhance antigenicity. Examples of linker sequences include, but are not limited to, an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, and a protein purification ligand.
  • LINKER a program to generate linker sequences for fusion proteins.
  • Protein Engineering 13(5): 309-312 which is a reference that describes unstructured linkers.
  • Structured (e.g. helical) sequence linkers may also be designed using, for example, existing sequences that are known to have that secondary structure, or using basic known biochemical principles to design the linkers.
  • Tags Recombinant protein sequences that can be added to the N- or C-terminus of a
  • recombinant protein tags examples include, but are not limited to, glutathione-S-transferease (GST), poly-histidine, maltose binding protein (MBP), FLAG, V5, halo, myc, hemaglutinin (HA), S-tag, calmodulin, tag, streptavidin binding protein (SBP), SoftaglTM, Softag3TM, Xpress tag, isopeptag, Spy Tag, biotin carboxyl carrier protein (BCCP), GFP, Nus-tag, strep-tag, thioredoxin tag, TC tag, and Ty tag. All such tags are well-known to those of ordinary skill in the art of recombinant protein production.
  • Protein Generally means a linear sequence of about 100 or more amino acids covalently joined by peptide bonds.
  • Polypeptide Generally means a linear sequence of about 55 to about 100 amino acids covalently joined by peptide bonds.
  • Peptide Generally means a linear sequence of about 55 or fewer amino acids covalently joined by peptide bonds.
  • peptide may be used interchangeably herein.
  • Chimeric or fusion peptide or polypeptide a recombinant or synthetic peptide or polypeptide whose primary sequence comprises two or more linear amino acid sequences which do not both occur in a single molecule in nature, or at least are not linearly contiguous in a single molecule in nature. (For example, they may both be present in a single molecule which comprises e.g.
  • the two or more sequences may be, for example, a peptide (e.g. an epitope or antigenic region) and a linker sequence, or two or more peptides (which may be the same or different) which are either contiguous or separated by a linker sequence, etc.
  • Tandem repeats two or more copies of nucleic acid or amino acid sequences encoding the same peptide, which are arranged in a linear molecule and are either contiguous or separated by a linker sequence, etc.
  • Original or native or wild type sequence The sequence of a peptide, polypeptide, protein or nucleic acid as found in nature.
  • Recombinant peptide, polypeptide, protein or nucleic acid peptide, polypeptide, protein or nucleic acid that has been produced and/or manipulated using molecular biology techniques such as cloning, polymerase chain reaction (PCR), etc.
  • Synthetic peptide, polypeptide, protein or nucleic acid peptide, polypeptide, protein or nucleic acid that has been produced using chemical synthesis procedures.
  • Type-specific associated primarily with a single phyletic group.
  • Surface protein A protein located on the outer surface membrane of a cell or bacterium.
  • Tables 1 , 2 and 3 provide a list of the SEQ ID NOS: for sequences described herein.
  • VIVVEFAHIPRSGVADMHAPVASSITS ENSNASAEGEDMEASEFSSAIAN
  • SEQ ID E. canis Ecaj_0636 MADDEYKGVIQQYINTVKEIVSDSKTF
  • SEQ ID E. canis Ecaj_0636 MADDEYKGVIQQYINTVKEIVSDSKTF
  • SEQ ID E. canis Ecaj_0636 HKVLKPKEGA
  • SEQ ID E. canis Ecaj_0563 MKHKLVFIKFILLCLILS S CKTTDH VPL V
  • SEQ ID E. canis Ecaj_0563 TTDHVPLVNTDHVFSNMKTIEKIYFDFG NO:49 KATIGDSDKAILE VIQKAQKDTNTNIVI
  • SEQ ID E. canis Ecaj_0563 GDSDKAILEKVIQ AQ DTNTNIVIV NO:50
  • SEQ ID E. canis Ecaj_0563 GHTDTRGTDEYNLELGE
  • SEQ ID E. canis Ecaj_0563 QRANAVKDFIIEHDKSLENRITVQSKGKS
  • SEQ ID E. canis Ecaj_0563 LVYS SNPEE AEHAH AKNRRVVI
  • Aph surface proteins AipA, OmpA, and Asp 14 have been identified as mediating bacteria-host cell binding and entry.
  • the surface proteins AipA, OmpA, and Asp 14 and fragments thereof that encompass an invasin domain are used for diagnosing whether a patient has been suffering from Aph infection.
  • antibodies to AipA are present in a suitable biological sample from a subject, then it can be concluded that the subject has been exposed to and/or is infected wi i Aph.
  • antibodies to AipA and one or both of OmpA and Asp 14 are identified in a suitable biological sample from a subject, then it can be concluded that the subject has been exposed to and/or is infected with ⁇ ijo z.
  • determinations are made, e.g. by testing serum or other biological material from a subject suspected of on Aph infection by a suitable assay, such as an ELISA or immunoblot using, for example, antibodies to (e.g. specific antibodies to AipA (and optionally, to OmpA, and Asp 14).
  • antibodies can also be raised against two or more of AipA, OmpA and Asp 14, or to invasin domains from two or more of AipA, OmpA and Aspl4, e.g. combined in a chimeric protein. If the antibodies bind to or interact with proteins or peptide fragments in the sample, then it can be determined that the subject has been exposed to, infected with, and/or is currently infected with Aph. Alternatively, if one or more of the amino acid sequences described herein (e.g.
  • administration of AipA protein or suitable antigenic fragments thereof, or nucleic acids encoding the protein or fragments is used to elicit an immunogenic response, e.g. a protective immunogenic response, in a subject.
  • the immunogenic response may protect a naive subject from subsequent full-blown Aph infection when exposed to the bacterium (e.g. when bitten by an infected tick).
  • Antibodies elicited by AipA administration bind to invasin proteins of bacteria that enter the bloodstream and prevent host cell invasion and establishment of a productive infection.
  • AipA protein or suitable antigenic fragments thereof, or nucleic acids encoding the protein or fragments is used to provide treatment for an existing Aph infection by eliciting the production of antibodies to Aph.
  • one or both of OmpA and Asp 14 proteins or suitable antigenic fragments thereof, or nucleic acids encoding such proteins and fragments may be administered.
  • all three proteins, or suitable effective fragments thereof as described herein, may be administered.
  • a polypeptide or protein comprising at least one "core" sequence or segment consisting of an invasin domain sequence from one or more of AipA, Asp 14 and OmpA as described herein.
  • the core sequence(s) is/are flanked on at least one of the amino terminus and carboxy terminus (i.e. at one or the other or both termini) by a sequence which is not found adjacent to the core sequence in nature.
  • the sequence which is not found adjacent to the core sequence in nature may be a heterologous sequence (e.g. synthetic and/or from another species or strain) or may be a repeat or duplication of the same sequence.
  • one core sequence is present alone; in other constructs, multiple core sequences of a single type (i.e. AipA, Asp 14 or OmpA) are present. In yet other constructs, one core sequence from at least two of AipA, Asp 14 and OmpA are present. In yet other constructions, multiple copies of one or more core sequence from at least two of AipA, Asp 14 and OmpA are present.
  • any protein sequence, as well as its corresponding nucleic acid sequence coding for the protein sequence that is or includes SEQ ID NO: 14 may be used as described herein.
  • sequences include one or more amino acids or amino acid sequences which are adjacent to or which flank SEQ ID NO: 14 in nature (such as those which are present in SEQ ID NO: 13).
  • polypeptide sequences as described herein may also be shortened on either the amino or carboxy terminus (or both) by one, two, or more amino acids to produce fragments within the context of the invention wherein the fragments produce the same or a similar protective effect.
  • the polypeptide may be a chimera or fusion protein which comprises flanking amino acids sequences which are not adjacent to the native sequence in nature.
  • the adjacent sequences may be corresponding amino acids which are from different but related species (e.g. other
  • Anaplasmataceae or amino acids which are from different species (e.g. from other bacteria or eukaryotes of interest, e.g. from infectious agents); or from a synthetic sequence, e.g. various tags such as histidine or glutathione S-transferase (GST) tags, linkers, spacers, targeting sequences, etc. as described elsewhere herein).
  • GST glutathione S-transferase
  • a single recombinant polypeptide may comprise one copy, or more than one copy (a plurality, two or more) of each of these different sequences, all of which include SEQ ID NO: 14.
  • a single polypeptide may contain two or more copies of a single sequence, a single copy of one sequence and two or more copies of one or more different sequences, or two or more copies of at least two different sequences.
  • a polypeptide of the invention includes one or more copies of SEQ ID NO: 14 and optionally one or more copies of at least one of SEQ ID NO:03 and SEQ ID NO:06 on a single polypeptide or multiple polypeptides. Another aspect of the disclosure provides a mixture of at least two of any of the peptides and/or polypeptides described herein.
  • the composition consists of or comprises three polypeptides with amino acid sequences encoding an AipA, OmpA, or Asp 14 invasin domain.
  • the Asp 14 invasin domain lies within aal 13-124 (SEQ ID NO:03).
  • polypeptide fragments such as Aspl4 aalOl-124 (SEQ ID NO:02) or the full length protein (SEQ ID NO:01) are used.
  • the composition of the invention comprises the invasin domain of OmpA, which lies within aa59-74 (SEQ ID NO:04).
  • a larger fragment of OmpA encompassing aal 9-74 (SEQ ID NO:05), or the full length OmpA protein (SEQ ID NO:06) is used.
  • the invention is a vaccine for prevention or treatment of anaplasmosis and HGA.
  • One embodiment of the invention is a pharmaceutically acceptable composition comprising one or a plurality of any one of or a mixture of at least two amino acid sequences which are or include the amino acid sequences which are identified as SEQ ID NO:01 , 02, 03, 04, 05, 06, 13, 14, or 15.
  • Administration of the composition of the invention stimulates an immune response in a subject and production of antibodies against AipA, Asp 14, OmpA, or all three.
  • AipA, OmpA, and Asp 14 are on the outer surface of Aph bacteria, antibodies produced by the subject will block binding of bacteria to host cells and interfere with uptake into vacuoles. Bacteria unable to enter host cells are detected by the host immune system and cleared from the body. Blockade can occur at the point of entry into neutrophils or endothelial cells or transfer between these two host cell types. Interruption of the zoonotic life cycle provides a further benefit to public health and well-being by breaking the chain of disease transmission to others.
  • the invention provides a method to detect the presence of Aph AipA, OmpA, and/or Asp 14 in assays of biological samples obtained from subjects to bind to antibodies produced by an Anaplasmataceae-infected individual, either of which would be diagnostic for HGA or anaplasmosis.
  • the preferred composition for diagnostic testing may comprise full length AipA (SEQ ID NO: 13), Asp 14 (SEQ ID NO: 3), and/or OmpA (SEQ ID NO:06).
  • compositions comprising fragments of AipA such as SEQ ID NO: 14 or a mixture of at least two of SEQ ID NO: 13, 14, and 15 are also contemplated.
  • a composition comprising fragments of OmpA, such as SEQ ID NO:04 and/or 05, and any mixtures of at least two of SEQ ID NO:04, 05, and 06 are also contemplated.
  • Fragments of Aspl4, such as SEQ ID NO:01 and/or 02 are also contemplated, as are any mixtures of at least two of SEQ ID NO:01, 02, and 03.
  • the assay used to detect antibodies may be any type of immunoassay, such as an immunoblot or an enzyme-linked immunosorbent assay.
  • the test sample may be any type of body fluid, such as blood, plasma, serum, urine, saliva, or other body fluid. Tissues or cells may also be used, such as tissue sections or cell preparations adhered to slides or coverslips for immunohistochemical staining.
  • the assay used is an ELISA with each protein type to independently detect antibodies to AipA, Asp 14, and OmpA, however, a combination to detect AipA, Asp 14, and OmpA antibodies in one ELISA is also contemplated.
  • Table 4 shows examples of proteins that can be included in the "cocktail" of peptides, polypeptides or protein sequences of the composition of the invention. Examples of these include APHJ325 (Msp2), APHJ235, APHJ378, APHJ412, APH_0346, APH_0838, APH_0839, APH_0874, and APH_0906 because all are upregulated 3- to 60-fold during RC-DC transition, DC exit, and/or reinfection and our surface proteomic study indicates that they are surface proteins.
  • the file names for each of the aforementioned proteins are from the A.
  • APH_1235 is another late stage gene that is upregulated 70-fold (Troese et al., 201 1).
  • APH_1235 is an A. phagocytophilum surface protein.
  • P44 is a 44 kilodalton surface protein and is the bacterium's major surface protein. Synonyms of P44 are Msp2 (major surface protein 2) and Msp2 (P44). All Anaplasma species encode P44 proteins and there are huge repertoires of P44 genes in these bacterial species' chromosomes. For instance, the annotated Aph strain HZ genome encodes 113 P44 proteins.
  • sequences from Aph surface proteins include but are not limited to antigenic peptide sequences such as linker sequences which in and of themselves are antigenic.
  • GST glutathione-S-transferease
  • MBP maltose binding protein
  • FLAG V5
  • halo myc
  • HA hemaglutinin
  • SBP streptavidin binding protein
  • SoftaglTM Softag3TM
  • Xpress tag isopeptag
  • Spy Tag biotin carboxyl carrier protein (BCCP)
  • BCCP biotin carboxyl carrier protein
  • linker sequences include but are not limited to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, and a protein purification ligand. It should also be recognized that a multitude of other such sequences are known to those of skill in the art, and inclusion of other antigenic, linker, or tag sequences is contemplated.
  • amino acid sequences that are chosen for inclusion in the polypeptides of the invention correspond exactly to the primary amino acid sequence of the original or native sequences of an AipA, Aspl 4, or OmpA protein, this need not always be the case.
  • the amino acid sequence of an epitope that is included in the polypeptides of the invention may be altered somewhat and still be suitable for use in the present invention. For example, certain conservative amino acid substitutions may be made without having a deleterious effect on the ability of the polypeptides to elicit an immune response.
  • substitution of a positively charged amino acid for another positively charged amino acid e.g. for R or vice versa
  • substitution of a negatively charged amino acid for another negatively charged amino acid e.g. D for E or vice versa
  • substitution of a hydrophobic amino acid for another hydrophobic amino acid e.g. substitution of A, V, L, I, W, etc. for one another; etc. All such substitutions or alterations of the sequences of the polypeptides that are disclosed herein are intended to be encompassed by the present invention, so long as the resulting polypeptides still function to elicit a suitable immune response.
  • amino acid sequences that are included in the polypeptides or any chimeric proteins of the invention need not encompass a full length native polypeptide.
  • amino acid sequences that are known to be or to contain antigenic polypeptides may, for a variety of reasons, be preferable for use in the practice of the invention, so long as the criteria set forth for an epitope is fulfilled by the sequence.
  • Amino acid sequences that are so substituted or otherwise altered may be referred to herein as "based on” or “derived from” the original wild type or native sequence.
  • the AipA, Asp 14, or OmpA proteins or polypeptide fragments from which the linear epitopes are "derived” or on which the linear epitopes are “based” are the AipA, Aspl4, or OmpA proteins or peptide fragments as they occur in nature.
  • These natural AipA, Asp 14, or OmpA proteins may alternatively be referred to as native or wild type proteins.
  • Such changes to the primary sequence may be introduced for any of a variety of reasons, for example, to eliminate or introduce a protease cleavage site, to increase or decrease solubility, to promote or discourage intra- or inter-molecular interactions such as folding, ionic interactions, salt bridges, etc, which might otherwise interfere with the presentation and accessibility of the individual epitopes along the length of a peptide or polypeptide. All such changes are intended to be encompassed by the present invention, so long as the resulting amino acid sequence functions to elicit a protective antibody response in a host to whom it is administered.
  • substituted sequences will be at least about 50% identical to the corresponding sequence in the native protein, preferably about 60 to 70, or even 70 to 80, or 80 to 90% identical to the wild type sequence, and preferably at least about 95, 96, 97, 98, 99, or even 100% identical to a native AipA, Asp 14, or OmpA sequence or peptide fragment.
  • the reference native AipA, Asp 14, or OmpA sequence or peptide fragment may be from any suitable type of Anaplasmataceae, e.g. from any Anaplasmataceae which is known to infect mammals.
  • individual linear epitopes in a chimeric vaccinogen are separated from one another by intervening sequences that are more or less neutral in character, i.e. they do not in and of themselves elicit an immune response to Anaplasmataceae.
  • sequences may or may not be present between the epitopes of a chimera. If present, they may, for example, serve to separate the epitopes and contribute to the steric isolation of the epitopes from each other.
  • sequences may be simply artifacts of recombinant processing procedures, e.g. cloning procedures.
  • Such sequences are typically known as linker or spacer peptides, many examples of which are known to those of skill in the art. See, for example, Crasto, C. J. and J. A. Feng. 2000.
  • chimeric proteins may be present in chimeric proteins, for example leader sequences or sequences that "tag" the protein to facilitate purification or detection of the protein, examples of which include but are not limited to tags that facilitate detection or purification (e.g. S-tag, Flag-tag, Histidine tags,), other antigenic amino acid sequences such as known T-cell epitope containing sequences and protein stabilizing motifs, etc.
  • tags that facilitate detection or purification e.g. S-tag, Flag-tag, Histidine tags,
  • the chimeric proteins may be chemically modified, e.g. by amidation, sulfonylation, lipidation, or other techniques that are known to those of skill in the art.
  • the invention further provides nucleic acid sequences that encode the proteins, protein fragments, polypeptides, peptides and/or chimeric proteins described herein.
  • nucleic acids include DNA, cDNA, RNA (e.g. mRNA), and/or hybrids thereof, and the like, both single and double stranded, and complements thereof.
  • the invention comprehends vectors which contain or house one or more of such coding sequences, or sequences complementary to the coding sequences. Examples of suitable vectors include but are not limited to plasmids, cosmids, viral based vectors, expression vectors, etc. In some embodiments, the vector is a plasmid expression vector.
  • the vector is a vector suitable for administration to a mammal such as a human, e.g. an attenuated viral vector such as an adenoviral, retroviral, herpes simplex, or other viral vector which is replication competent.
  • the vector is a host cell harboring the nucleic acid.
  • the encoding sequences are typically configured within the vectors so as to be translatable, e.g. by being operationally connected to a functional promoter sequence or region.
  • the vectors may or may not be capable of replication, but are capable of being translated to produce the encoded polypeptide(s).
  • Nucleic acids encoding the sequences of interest may also be present in or associated with, for example, lipoplexes, polymersomes, polyplexes, dendrimers, organic and inorganic nanoparticles, etc.
  • the invention also provides host cells containing the nucleic acids of the invention.
  • the host cell can be any cell that can grow in culture, be transformed or transfected with
  • heterologous nucleotide sequences and can express those sequences. These include bacteria, such as E. coli, Bacillus, yeast and other fungi, plant cells, insect cells, and mammalian cells. In addition, expression of these sequences in higher eukaryotic host cells may be transient or stable.
  • the chimeric proteins of the invention may be produced by any suitable method, many of which are known to those of skill in the art. For example, they may be chemically synthesized, or produced using recombinant DNA technology (e.g. in bacterial cells, in cell culture (mammalian, yeast or insect cells), in plants or plant cells, or by cell-free prokaryotic or eukaryotic-based expression systems, by other in vitro systems, etc.). In some embodiments, the polypeptides are produced using chemical synthesis methods.
  • compositions for use in eliciting an immune response comprise one or more peptides, polypeptides, or proteins as described herein, or nucleic acids encoding them (e.g. as the nucleic acid per se, or in a vector such as a host cell comprising the nucleic acid).
  • the compositions may be utilized as
  • immunogenic composition and/or vaccines to prevent or treat anaplasmosis, particularly when manifested in humans as HGA.
  • eliciting an immune response it is meant that administration of the antigen causes the synthesis of specific antibodies (at a titer as described above) and/or cellular proliferation, as measured, e.g. by 3 H thymidine incorporation, or by other known techniques.
  • vaccine we mean a linear polypeptide, a mixture of linear polypeptides or a chimeric or fusion polypeptide that elicits an immune response, which results in protection of an organism against challenge with an Anaplasmataceae species bacterium. The protective response either wholly or partially prevents or arrests the development of symptoms related to
  • compositions include one or more isolated and substantially purified polypeptides or chimeric peptides as described herein, and a pharmacologically suitable carrier.
  • the polypeptides or chimeric peptides in the composition may be the same or different, i.e. the composition may be a "cocktail" of different polypeptides or chimeric peptides, or a composition containing only a single type of polypeptide or chimeric peptide.
  • compositions for use as vaccines are well known to those of skill in the art.
  • such compositions are prepared either as liquid solutions or suspensions, however solid forms such as tablets, pills, powders and the like are also contemplated.
  • the liquid may be an aqueous liquid.
  • Solid forms suitable for solution in, or suspension in, liquids prior to administration may also be prepared.
  • the preparation may also be emulsified.
  • the active ingredients may be mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredients. Suitable excipients are, for example, water, saline, dextrose, glycerol, ethanol and the like, or combinations thereof.
  • compositions may contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, and the like.
  • the vaccine preparations of the present invention may further comprise an adjuvant, suitable examples of which include but are not limited to Seppic, Quil A, Alhydrogel, etc. If it is desired to administer an oral form of the composition, various thickeners, flavorings, diluents, emulsifiers, dispersing aids or binders and the like may be added.
  • the composition of the present invention may contain any such additional ingredients so as to provide the composition in a form suitable for administration.
  • the final amount of polypeptides or chimeric peptides in the formulations may vary. However, in general, the amount in the formulations will be from about 0.01-99%, weight/volume.
  • the methods involve administering a composition comprising proteins, protein fragments (peptides), recombinant polypeptides and/or chimeric peptides described herein, or by
  • nucleic acids encoding these proteins, fragments and recombinant polypeptides and chimeric peptides, in a pharmacologically acceptable carrier to a mammal.
  • the nucleic acids may be housed in a vector (e.g. a host cell) as described herein.
  • the mammal may be a human, but this need not always be the case. Because anaplasmosis is a zoonotic disease that causes anaplasmosis in all known mammalian hosts, veterinary applications of this technology are also contemplated.
  • the vaccine preparations of the present invention may be administered by any of the many suitable means which are well known to those of skill in the art, including but not limited to by injection, inhalation, orally, intranasally, by ingestion of a food product containing the polypeptides or chimeric peptides, etc.
  • the mode of administration is subcutaneous or intramuscular.
  • the compositions may be administered in conjunction with other treatment modalities such as substances that boost the immune system, various anti- bacterial chemotherapeutic agents, antibiotics, and the like.
  • the present invention provides methods to elicit an immune response to
  • Anaplasmataceae and/or to vaccinate against Anaplasmataceae infection in mammals are a human.
  • the mammal is a human.
  • the preparations may also be used for veterinary purposes. Examples include but are not limited to companion "pets" such as dogs, cats, etc.; food source, work and recreational animals such as cattle, horses, oxen, sheep, pigs, goats, and the like; or wild animals that serve as a reservoir of Anaplasmataceae, particularly wild animals adapted to living in close proximity to urban areas (e.g. mice, deer, rats, raccoons, opossum, coyotes, etc).
  • compositions of the invention may be used as bait vaccines, such as bait containing any of the vaccine preps described herein (e.g. mouse bait, deer bait, etc.) Also, animals in zoos and reserves may benefit from protection against Anaplasmataceae infection.
  • the invention also provides a diagnostic and a method for using the diagnostic to identify individuals who have antibodies to the epitopes contained within the polypeptides or chimeric proteins of the invention.
  • a biological sample from an individual e.g. a human, a deer, or other mammals susceptible to infection by Anaplasmataceae
  • Anaplasmataceae suspected of having been exposed to Anaplasmataceae, or at risk for being exposed to Anaplasmataceae
  • the peptides, polypeptides, or chimeric proteins of the invention is contacted with the peptides, polypeptides, or chimeric proteins of the invention.
  • a positive result i.e.
  • Anaplasmataceae indicates that the individual has been exposed to and has previously mounted an immune response to, and/or is infected with Anaplasmataceae.
  • the diagnostic aspects of the invention are not confined to clinical use or home use, but may also be valuable for use in the laboratory as a research tool, e.g. to identify Anaplasmataceae bacteria isolated from ticks, to investigate the geographical distribution of Anaplasmataceae species and strains, etc.
  • the present invention also encompasses antibodies to the epitopes and/or to the polypeptides or chimeric proteins disclosed herein.
  • Such antibodies may be polyclonal, monoclonal, or chimeric, and may be generated in any manner known to those of skill in the art.
  • the antibodies are bactericidal, i.e. exposure of
  • Anaplasmataceae bacteria to the antibodies causes death of the bacteria.
  • Such antibodies may be used in a variety of ways, e.g. as detection reagents to diagnose prior exposure to
  • Anaplasmataceae as a reagent in a kit for the investigation of Anaplasmataceae, to treat Anaplasmataceae infections, etc.
  • antigen fragments or antigenic sequences or epitopes may be identified by their ability, when included in polypeptides or chimeric proteins, to elicit suitable antibody production to the epitope in a host to which the polypeptides or chimeric proteins are administered.
  • suitable antibody production is characterized by an antibody titer in the range of from about 100 to about 100,000, and preferably in the range of from about 10,000 to about 10,000,000.
  • the "titer" should be about three times the background level of binding.
  • reactivity in a test should be at least three times greater than reactivity detected in serum from uninfected individuals.
  • the antibody response is protective, i.e. prevents or lessens the development of symptoms of disease in a vaccinated host that is later exposed to Anaplasmataceae, compared to an unvaccinated host.
  • Anaplasma phagocytophilum surface protein AipA mediates invasion of mammalian host cells
  • Anaplasma phagocytophilum which causes granulocytic anaplasmosis in humans and animals, is a tick-transmitted obligate intracellular bacterium that mediates its own uptake into neutrophils and non-phagocytic cells. Invasins of obligate intracellular pathogens are attractive targets for protecting against or curing infection because blocking the internalization step prevents survival of these organisms.
  • the complement of A. phagocytophilum invasins is incompletely defined.
  • AipA the significance of a novel A. phagocytophilum invasin protein, AipA is reported.
  • the bacterium upregulated aipA transcription when it transitioned from its non-infectious reticulate cell morphotype to its infectious dense-cored morphotype during infection of HL-60 cells.
  • AipA localized to the bacterial surface and was expressed during in vivo infection.
  • residues 1 to 87 residues 1 to 87 (AipAi-87) were found to be essential for host cell invasion.
  • Recombinant AipAi-8 7 protein bound to and competitively inhibited A. phagocytophilum infection of mammalian cells. Antiserum specific for AipAi -87 , but not other AipA regions, antagonized infection.
  • Anaplasma phagocytophilum is an obligate intracellular bacterium in the order
  • HGA human granulocytic anaplasmosis
  • the disease presents as a non-specific febrile illness that can be accompanied by leukopenia, thrombocytopenia, elevated levels of serum
  • HGA is an emerging infection in the United States, Europe, and Asia (Truchan et al, 2013). The number of reported HGA cases in the United States increased over six-fold from
  • DC dense-cored
  • RC non-infectious reticulate cell
  • phagocytophilum OMPs that are upregulated during RC-to-DC transition, bacterial exit, and reinfection are attractive targets to evaluate for both their roles in infection and their prospect as protective antigens.
  • OmpA (APH0338) and Aspl4 (14-kDa A phagocytophilum surface protein; APH0248) were recently identified as being important for A. phagocytophilum entry into mammalian cells (Ojogun et at, 2012; Kahlon et at, 2013).
  • OmpA binds to a2,3-sialic acid of the sialyl Lewis x (sLe x ) tetrasaccharide that caps P-selectin glycoprotein ligand-1 (PSGL-1) on myeloid cell surfaces.
  • SGL-1 P-selectin glycoprotein ligand-1
  • a whole genome transcriptional profiling study revealed A. phagocytophilum genes that are upregulated during infection of mammalian versus tick cells (Nelson et at, 2008). Several of these encode putative OMPs, one of which is APH0915. In this study, it is shown that APH0 15, hereafter referred to as AipA (A. phagocytophilum invasion protein A), is important for bacterial entry into mammalian cells and identify its invasion domain. It is further demonstrated that a combination of antisera targeting AipA, OmpA, and Asp 14 synergistically blocks infection.
  • the AipA sequence was assessed for transmembrane domains using the TMpred and TMHMM algorithms (Hofmann et at, 1993; Krogh et at, 2001), each of which yielded highly similar predictions. Results obtained using TMpred are presented in Figure 1.
  • Protean which is part of the Lasergene® software package (version 8.02; DNASTAR®, Madison, WI), was used to assess AipA for regions of hydrophobicity and probability of being surface-exposed using the Kyte-Doolittle (Kyte et at, 1982) and Emini (Emini et at, 1985) algorithms, respectively. Recombinant protein and antiserum production
  • nucleotides 745 to 1068 encoding AipA amino acids 249 to 355, was generated using primers 5 ' -CACCATCTATCAAGGAAATTACGAAGATCGC AAC-3 ' (SEQ ID NO:71) and 5'- GAGCAGCATGCTTTA-3 ' (SEQ ID NO:72).
  • the amplicons were cloned into the pDest-15 vector (Life Technologies, Carlsbad, CA) downstream of and in frame with the gene encoding GST as described previously (Ojogun et al., 2012). Expression and purification GST-tagged AipA residues 1 to 87 (GST-AipAi.
  • Rabbit polyclonal antisera were raised against synthetic keyhole limpet hemocyanin ( LH)-conjugated peptides corresponding to AipA amino acid residues 9 to 21, 61 to 84, 165 to 182, and 185 to 201 (New England Peptide, Gardner, MA). Specificity of each AipA peptide antiserum for its target peptides was determined by the enzyme-linked immunosorbent assay using the TMB substrate kit (Thermo Scientific, Waltham, MA) according to the manufacturer's instructions.
  • LH keyhole limpet hemocyanin
  • HL-60 cells were synchronously infected with A. phagocytophilum DC organisms (Troese et al, 2009). The infection time course was allowed to proceed for 36 h, a time period that enabled the bacteria to complete their biphasic developmental cycle and initiate a second round of infection (Troese et al, 2009).
  • RNA isolated from aliquots taken every 4 hours was subjected to reverse transcriptase-quantitative PCR (RT-qPCR) as described previously (Troese et al, 2011) using AipA specific primers 5 ' -CCTCAACTAAAGAAGCGTC ATC AAA-3 ' (SEQ ID NO:73) and 5 '-GTACGGTGTACAAAACGAGGAACA-3 ' (SEQ ID NO:74), which targeted nucleotides 179 to 388. Relative aipA transcript levels were normalized to the transcript levels of the A.
  • phagocytophilum 16s rRNA gene (aphlOOO) using the 2 'AACT method (Livak et al, 2001 ; Kahlon et al, 2013).
  • normalized aipA transcript levels were calculated as fold changes in expression relative to expression at 16 h, a time point at which the entire A. phagocytophilum population existed in the RC form (Troese et al, 2009; Mastronunzio et al, 2012). aipA expression during blood meal acquisition by A.
  • phagocytophilum infected nymphs from mice was monitored as described (Mastronunzio et al, 2012) using AipA primers targeting nucleotides 179 to 388.
  • expression of the A. phagocytophilum groEL gene was monitored as described (Mastronunzio et al, 2012) using AipA primers targeting nucleotides 179 to 388.
  • Sera from an HGA patient and a dog that had been naturally infected with A. phagocytophilum were previously described (Ojogun et al, 2012).
  • Western blot analyses (Troese et al, 2011) were performed on uninfected or A. phagocytophilum infected host cells or A phagocytophilum DC organisms that had been subjected to surface trypsinolysis as described previously (Kahlon et al, 2013).
  • A. phagocytophilum infected host cells were analyzed by spinning disk confocal microscopy as described (Huang et al, 2010a; Huang et al, 2012).
  • each respective antiserum was at a concentration of 2 mg mL "1 , and control antiserum was matched accordingly.
  • bacterial adhesion to and infection of HL-60 cells were monitored using spinning disk confocal microscopy (Ojogun et al, 2012).
  • Mammalian host cells cells were incubated with 4 ⁇ GST, GST-AipAi -87 , or GST- AipA 24 9 -3 55 for 1 h at 37°C. Spinning disk confocal microscopy and flow cytometry were used to assess the binding of recombinant proteins to host cells and competitive inhibition of A.
  • PSGL-1 DC organisms were incubated with antiserum targeting AipA] -87 , AipA 2 49- 3 55, OmpA (Ojogun et al, 2012), or preimmune control serum as described above.
  • the treated bacteria were incubated with PSGL-1 CHO cells or untransfected CHO cells for 1 h, followed by two rounds of washing with PBS to remove unbound bacteria, and enumeration of bound organisms using spinning disk confocal microscopy as described (Troese et al, 2009).
  • PSGL-1 CHO cells were incubated with the PSGL-1 N-terminus-specific antibody, KPL-1 (BD Biosciences, San Jose, CA), or the sLe x -specific antibody, CSLEX1 (BD Biosciences) for 30 min prior to the addition of bacteria.
  • KPL-1 BD Biosciences, San Jose, CA
  • CSLEX1 BD Biosciences
  • ANOVA One-way analysis of variance
  • A. phagocytophilum differentially expresses AipA during the infectious stage of its biphasic developmental cycle, the transmission bloodmeal of infected ticks, and infection of mammalian hosts
  • AipA is a 355-amino acid (36.9 kDa) protein and a putative OMP (Nelson et al, 2008).
  • OMP Organic et al, 2008.
  • residues 107 to 127, 136 to 155, and 220 to 243 form transmembrane domains that position residues 1 to 106 and 156 to 219 on the bacterial surface ( Figure 1 A).
  • Protein BLAST searches revealed that AipA does not display high sequence identity to sequenced proteins of other organisms, including
  • Anaplasmataceae and Rickettsiales members are important for infection (Huang et al, 2010b; Troese et al, 2011 ; Mastronunzio et al, 2012), AipA expression throughout the infection cycle in human promyelocytic HL-60 cells was examined.
  • aipA mRNA levels were approximately 10- to 20-fold higher between 30 and 36 hours - time points that correspond to RC-to-DC transition, exit, and reinfection - than between 4 and 26 hours, time points that correspond to conversion to and replication of non-infectious RC organisms (Troese et al, 2009) ( Figure 2A). Also, the aipA mRNA level of the DC inoculum was comparable to that detected at 36 h, a time point that correlates with reinfection.
  • AipA amino acids 1 - 87 (AipAi-87), 165-204 (AipA[65-204) and 249-355 (A1PA249-355) contain segments that are hydrophilic and predicted to be accessible on the protein's surface (Figure IB).
  • AipAi.8 7 and AipAi 65 -204 are predicted to be exposed on the bacterial surface, while AipA 2 4 - 3 5 is not ( Figure IB).
  • AipAi-87 and AipA 249 -3 55 were expressed in Escherichia coli as proteins N-terminally fused to glutathione-5-transferase. Despite numerous attempts, soluble GST-tagged full-length AipA and AipAi 65-2 o 4 could not be expressed (data not shown).
  • Mouse antiserum raised against AipAj. 87 and AipA 24 9-35 5 fusion proteins recognized a band of the expected size in lysates of A.
  • phagocytophilum infected but not uninfected HL-60 cells (Figure 2B).
  • Anti-AipA2 4 9- 355 recognized an additional band having an apparent mobility that was slightly smaller than 75 kDa, suggesting that AipA may dimerize.
  • anti-AipA 24 9 -3 5 5 may recognize an epitope that is shared with or is similar in sequence to that of the unknown A. phagocytophilum protein.
  • An AipAi-87 antibody was used to screen infected HL-60 cells by immunofluorescence microscopy. Consistent with the transcriptional data, approximately 70% and 96% of the A.
  • phagocytophilum inclusions contained AipA-expressing bacteria at 28 and 32 h, respectively, whereas considerably fewer inclusions were AipA-positive at earlier time points (Figure 2C). These data demonstrate that AipA is both transcriptionally and translationally upregulated during periods when A. phagocytophilum converts to and is in its infectious DC morphotype.
  • groEL aph0240 expression was also examined. groEL was detected at the highest level in infected unfed nymphs and decreased in expression over the duration of the bloodmeal. Thus, A. phagocytophihim specifically induces select genes, including aipA, as it adapts during the transmission bloodmeal to colonize the mammalian host.
  • A. phagocytophihim preferentially expresses aipA during growth in HL-60 cells versus I. scapidaris embryo-derived ISE6 cells (Nelson et al., 2008), AipAi_87 antibody failed to detect a protein of the expected size in lysates of ISE6 cells in which A. phagocytophihim had been continually passaged for one, six, or seven weeks (Figure 2E).
  • APH0032 which is an A. phagocytophihim protein that was previously demonstrated to be expressed during infection of ISE6 cells (Huang et al., 2010b), was detected in all infected samples. HGA patient serum and serum from a dog that had been naturally infected with A.
  • AipA is an A. phagocytophilum surface protein
  • confocal microscopy was used to screen infected RF/6A endothelial cells with AipAi_ 87 antibody in conjunction with antiserum targeting the A. phagocytophilum major surface protein, Msp2 (P44) (Carlyon, 2012). Both antibodies detected intravacuolar organisms, yielding the ring-like staining pattern on their peripheries that is characteristic for Msp2 (P44) and other confirmed A. phagocytophilum OMPs (Ge et al, 2007; Ojogun et al, 2012; Kahlon et al, 2013) ( Figure 3A).
  • AipA residues 1 to 87 include six lysine and three arginine residues, making this putative surface exposed region susceptible to tryptic digest. If AipAi -87 or portions thereof were exposed on the A.
  • GST-AipA requires amino acids 1 to 87 to bind and competitively inhibit A. phagocytophilum infection of mammalian host cells
  • AipA is an exposed surface protein that is induced during key infection stages in the A. phagocytophilum life cycle. It was assessed if GST-tagged AipA( -8 7 and AipA 24 9- 3 55 bind to RF/6A cells. GST alone served as a negative control. GST antibody detected GST-AipAi -87 that had adhered to the host cells by both immunofluorescence microscopy and flow cytometry ( Figure 4, A and B). GST-AipA 24 9- 35 5 bound poorly, at best, and GST alone did not bind to host cells.
  • GST-AipAi_ 87 but not GST-AipA 24 9-35 5 would be able to serve as a competitive agonist to inhibit A. phagocytophilum infection.
  • -87 significantly reduced the percentages of infected cells and the number of bacterial inclusions per cell by nearly four-fold relative to incubation with GST alone ( Figure 4, C to F).
  • GST-AipA 2 49.3 55 did not inhibit A. phagocytophilum infection of HL-60 cells and reduced infection of RF/6A cells by only a small degree.
  • Antiserum targeting AipA residues 1 to 87 inhibits A. phagocytophilum infection of host cells Given that AipA amino acids 1 to 87 are exposed on the A. phagocytophilum surface and contribute to infection, it was assessed if treating DC organisms with heat-inactivated AipAi -87 antiserum prior to incubating them with HL-60 cells would inhibit infection. OmpA antiserum, for which its ability to inhibit A. phagocytophilum infection was previously validated (Ojogun et al, 2012), was a positive control. Anti-AipAi -87 and anti-OmpA each reduced the number of infected cells and the number of bacterial inclusions per cell by approximately 40% ( Figure 5, A and B).
  • AipA 24 9 -3 55 antiserum had no effect on A. phagocytophilum infection. Consistent with the studies of A. phagocytophilum invasins (Ojogun et al, 2012; Kahlon et al, 2013), neither AipAi -87 nor OmpA antiserum inhibited bacterial adhesion to HL-60 cells (data not shown).
  • AipA targets a Le ⁇ -capped PSGL-1 -independent receptor
  • sLe x -capped PSGL-1 is the only known A. phagocytophilum receptor on myeloid host cells (Herron et al, 2000), and OmpA binds the 2,3-sialic acid determinant of sLe x (Ojogun et al, 2012). Since A.
  • phagocytophilum interactions with sLe x -capped PSGL-1 involve at least one bacterial surface protein in addition to OmpA (Carl yon et al, 2003; Yago et al, 2003; Reneer et al, 2006; Sarkar et al, 2007; Reneer et al, 2008; Ojogun et al, 2012), it was investigated if AipAikos87 antiserum could inhibit bacterial binding to Chinese hamster ovary (CHO) cells transfected to express sLe x -capped PSGL-1 (PSGL-1 CHO cells).
  • CHO Chinese hamster ovary
  • phagocytophilum adhesion were PSGL-1 CHO cells that had been pretreated with KPL-1 or CSLEX1, which are monoclonal antibodies that block the bacterium's access to the PSGL-1 N-terminus or the a2,3-linked sialic acid determinant of sLe x , respectively (Goodman et al, 1999; Herron et al, 2000; Troese et al, 2009).
  • a combination of antisera targeting AipA, OmpA, and Aspl4 blocks A. phagocytophilum infection of host cells
  • A. phagocytophilum infection requires cooperative interactions of multiple invasins with the host cell surface (Truchan et al, 2013). Incubating DC organisms with antiserum targeting full-length OmpA (Ojogun et al, 2012), full-length Aspl4 (Kahlon et al, 2013), or AipAi- 87 significantly, but only partially, reduced A. phagocytophilum infection of mammalian host cells. It was therefore investigated if blocking multiple bacterial -host interactions by treating DC organisms with combinations of AipAi-g 7 , OmpA, and/or Aspl4 antisera could improve blocking efficacy.
  • Invasins of obligate intracellular pathogens are dualistic: they are essential for bacterial entry into host cells but, as such, they are also "Achilles' Heels" that can be blocked to prevent infection and pathogen survival.
  • Targeting invasins of arthropod-transmitted pathogens that are induced during the arthropod blood meal is an attractive approach because of its ability to prevent not only establishment of infection but also disease transmission. Given that A.
  • phagocytophilum infection is predicated on the cooperative actions of multiple bacterial surface- associated invasins (Truchan et al, 2013), most of which are induced during the tick
  • AipA is an attractive target to include in a multicomponent granulocytic anaplasmosis vaccine. It is an invasin that is induced during tick transmission feeding, is preferentially expressed during the bacterium's infectious stage, and functions synergistically with OmpA and Asp 14 to mediate optimal infection of mammalian host cells.
  • AipA The exposure of AipA on the infectious DC form surface makes it accessible to blocking antibodies. Indeed, preincubating DC organisms with AipA antiserum significantly reduced infection of HL-60 cells. Pretreatment of DC bacteria with a combination of antibodies targeting AipA, OmpA, and Asp 14 nearly abolished infection, whereas pretreatment with antibodies against one or two of the three invasins was less effective. Thus, AipA, OmpA, and Asp 14 are collectively critical for infection and targeting all three together blocks infection in vitro. Spotted fever group Rickettsia species, which are in the Order Rickettsiales with A. phagocytophilum, also use multiple surface proteins to promote entry into host cells (Martinez et al., 2004;
  • GST- AipA is capable of binding to mammalian cells, which suggests that it functions not only as an invasin but also as an adhesin. Yet, AipA antibodies and GST-AipAi -87 each failed to inhibit A. phagocytophilum binding to mammalian cells. In these experiments, the role of AipA as an adhesin was presumably masked by the presence of other adhesins/invasins, such as OmpA and Aspl4, on the bacterial surface (Ojogun et al, 2012; Kahlon et al, 2013). The AipA receptor is unknown. However, because AipA antibody failed to inhibit A.
  • AipA recognizes a sLe x -capped PSGL-1 independent receptor.
  • AipA and Asp 14 which also engages a sLe x -capped PSGL-1 independent receptor (Kahlon et al, 2013), complement the sLe x -targeting activity of OmpA (Ojogun et al, 2012).
  • Bacterial genes that are upregulated during transmission feeding of arthropod vectors are critical for various vector-transmitted bacteria to establish infection in their mammalian hosts (Hinnebusch et al, 1996; Perry et al, 1997; Grimm et al, 2004; Tilly et al, 2006). Consistent with these phenomena, aipA is not expressed by phagocytophilum during its residence in ISE6 cells or I. scapularis nymphs, but is induced when the bacterium is cultivated in mammalian tissue culture cells and during tick transmission feeding. Furthermore, A. phagocytophilum expresses AipA during infection of humans and dogs.
  • AipA is dispensable for bacterial colonization of the tick vector, but is important for infecting mammalian hosts. Similar expression profiles have been observed for the other identified invasins OmpA, Asp 14, and APH1235 (Mastronunzio et al, 2012; Ojogun et al, 2012; Kahlon et al, 2013). Also, like APH1235 (Troese et al, 201 1 ; Mastronunzio et al, 2012), AipA is pronouncedly upregulated when the bacterium is in the DC stage. In agreement with the invasive role of the DC
  • both proteins are important for establishing and/or maintaining infection in mammalian host cells.
  • AipA is transported to and associates with the bacterial outer membrane, as it lacks a canonical signal peptide that would target it for Sec-dependent or twin-arginine secretion. This conundrum is further complicated as AipA is unique to A. phagocytophilum and bears no homology to any known crystal structure. Perhaps AipA is an atypical transmembrane protein or a peripheral membrane protein that is anchored to the bacterial outer membrane via a posttranslational modification.
  • AipA colocalizes with the confirmed outer membrane protein, Msp2 (P44) and functions in concert with OmpA and Asp 14, both of which have also been shown to colocalize with Msp2 (P44) (Kahlon et al, 2013; Ojogun et al, 2012).
  • Msp2 (P44) has been proposed to form heteromeric complexes that mediate interactions with host cells (Park et al, 2003). Given that AipA, Aspl4, and OmpA synergistically promote A. phagocytophilum infection of host cells, it will be important to determine if they do so as a multimeric invasin complex that includes Msp2 (P44).
  • the AipA invasion domain lies within residues 9 to 21, which is a hydrophilic region of the protein that is exposed on the bacterial surface. Antiserum targeting this span reduces A. phagocytophilum infection of host cells by a level comparable to that achieved by antiserum targeting the entire surface-exposed N-terminal domain of AipA. The observed inhibition is specific to anti-AipA 9-2 i, as antisera targeting peptides corresponding to all other predicted hydrophilic regions of AipA exhibited no inhibitory effect.
  • Granulocytic anaplasmosis can be debilitating or fatal, and there is no vaccine that protects against the disease.
  • the results described herein show for the first time that
  • phagocytophilum invasins effectively blocks infection in vitro.
  • EXAMPLE 2 Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells.
  • Anaplasma phagocytophilum causes granulocytic anaplasmosis, an emerging disease of humans and domestic animals.
  • the obligate intracellular bacterium uses its invasins OmpA, Asp 14, and AipA to infect myeloid and non-phagocytic cells.
  • the present study identifies the OmpA binding domain as residues 59 to 74.
  • Polyclonal antibody generated against a peptide spanning OmpA residues 59 to 74 inhibited A. phagocytophilum infection of host cells and binding to its receptor, sialyl Lewis x (sLe x )-capped P-selectin glycoprotein ligand 1.
  • HGA Human granulocytic anaplasmosis
  • HGA can also be spread via perinatal, nosocomial, and blood transfusion routes (Bakken et al, 1998; Alhumaidan et al., 2013; Annen et al, 2012; Jereb et al., 2012). It is an acute illness characterized by fever, chills, headache, malaise, leukopenia, thrombocytopenia, and elevated liver enzymes. Complications can include shock, seizures, pneumonitis, rhabdomyolysis, hemorrhage, increased susceptibility to secondary infections, and death. Risk for complications and fatality is greater for the elderly, the immunocompromised, and when proper diagnosis and/or antibiotic therapy are delayed (Truchan et al., 2013).
  • the causative agent of HGA is Anaplasma phagocytophilum, an obligate intracellular bacterium that exhibits a tropism for neutrophils (Truchan et al., 2013).
  • A. phagocytophilum is carried by a variety of wild animal reservoirs and, in addition to humans, causes disease in domestic animals including dogs, cats, horses, and sheep (Stuen et ai, 2013).
  • A. phagocytophilum exhibits a biphasic developmental cycle similar to that of Chlamydia spp., Ehrlichia spp., and Coxiella burnetii (Bastidas et al., 2013; Minnick et al., 2012; Zhang et al., 2007; Troese et al., 2009).
  • the A. phagocytophilum infectious dense-cored (DC) form promotes its receptor-mediated uptake into a host cell derived vacuole.
  • the DC develops into the non-infectious reticulate cell (RC) form that replicates to form a bacterial cluster called a morula (Troese et ah, 2009; Ojogun et al., 2012). RCs then convert back to DCs and are released to initiate the next infection cycle (Troese et al., 2009).
  • RC non-infectious reticulate cell
  • Sialyl Lewis x [NeuAca(2-3)Gai i-4(Fucal-3)GlcNac]; sLe x ), an a2,3-5 sialylated and al ,3-fucosylated core-2 O-linked glycan that caps the N-termini of selectin ligands (Sperandio et al., 2006), is a critical A. phagocytophilum receptor (Goodman et al., 1999). sLe x is richly expressed on mammalian cells that are permissive for A.
  • phagocytophilum infection such as neutrophils, bone marrow progenitors, and promyelocytic HL-60 cells ( arakantza et al, 1994; Symington et al., 1985; Fukuda et al., 1984).
  • A. phagocytophilum recognizes sLe x that caps the N-terminus of P-selectin glycoprotein ligand-1 (PSGL-1) on these myeloid cells (Goodman et al, 1999; Herron et al., 2000).
  • Neutrophils and HL-60 cells that have been treated with an sLe x blocking antibody, from which surface sialic acids have been enzymatically removed, or that are devoid of sialyltransferase and/or l ,3-fucosyltransferase activity are resistant to A.
  • A.phagocytophilum also infects rhesus monkey choroidal (RF/6A) endothelial cells, megakaryoblastic MEG-01 cells, and bone marrow-derived mast cells in tissue culture.
  • RF/6A rhesus monkey choroidal
  • Infection of these non-myeloid host cell types depends on sLe x itself, 2,3-sialic acid, and/or l,3-fucose (Huang et al., 2012; Kahlon et al., 2013; Mastronunzio et al., 2012; Ojogun et al, 201 1).
  • sLe x and possibly other closely related a2,3-sialylated and ctl,3-fucosylated molecules are essential for efficient A phagocytophilum infection of mammalian cells.
  • phagocytophilum OmpA and a2,3-sialic acid was identified as the bacterium's first
  • adhesin/invasin-receptor pair (Ojogun et al., 2012).
  • OmpA binding to the a2,3-sialic acid determinant of sLe x on myeloid cells and to a2,3-sialylated glycans on RF/6A cells are vital steps in A. phagocytophilum invasion of these host cell types (Ojogun et al., 2012).
  • Exposure of OmpA on the A. phagocytophilum DC surface makes it accessible to antibodies (Ojogun et al, 2012), which could be used to exploit the bacterium's obligatory intracellular nature to block the host cell invasion step that is essential for survival.
  • the OmpA binding domain that recognizes a2,3-sialic acid lies within amino acids 19 to 74 (Ojogun et al., 2012), but has yet to be specifically identified.
  • the phagocytophilum OMP that recognizes l,3-fucose is unknown.
  • OmpA functions in concert with two additional invasins that are also upregulated during tick transmission feeding, Asp 14 (14-kDa A phagocytophilum surface protein) and AipA A. phagocytophilum invasion protein A), to promote optimal A.
  • the AipA binding domain was defined in Example 1 as residues 9 to 21.
  • phagocytophilum infected NASH-1 strain
  • HL-60 cells ATCC CCL- 240
  • RF/6A cells ATCC CRL-1790, Manassas, VA
  • CHO (-) and PSGL-1 CHO cells were cultivated as described (Li et al., 1996).
  • pGST-OmpA which encodes OmpAl 9-205 N-terminally fused to GST, was previously constructed (Ojogun et al, 2012). Using pGST-OmpA as the template, the QuikChange®
  • Lightning (Agilent Technologies, Santa Clara, CA) protocol was used per the manufacturer's guidelines to perform site-directed insertions and point mutagenesis of the ompA insert sequence.
  • a five-amino acid insert sequence (CLNHL; SEQ ID NO:75) was selected based on previous studies that had successfully employed the linker-scanning method (Anton et al, 2004; Okoye et al, 2006), which is used to insert peptide "linkers" to disrupt protein binding domains without perturbing overall protein structure.
  • CLNHL The sequence chosen for the insertion peptide, CLNHL (SEQ ID NO:75), was a consensus sequence based on the most common amino acids at their respective positions in the insertion peptides used in prior studies (Anton et al, 2004; Okoye et al, 2006).
  • the nucleotide sequence, 5'-TGCCTGAACCACCTG-3' (SEQ ID NO:76), which encoded CLNHL (SEQ ID NO:75) was inserted in the ompA sequence of pGST-OmpA between ompA nucleotides 102 and 103, 162 and 163, 186 and 187, 201 and 202, 216 and 217, and 231 and 232 to yield plasmids that encoded GST-OmpA proteins bearing CLNHL inserts between OmpA amino acids 34 and 35, 54 and 55, 62 and 63, 67 and 68, 72 and 73, and 77 and 78, respectively.
  • the QuikChange protocol was used to perform site directed mutagenesis to yield plasmids that encoded GST-OmpA proteins having R32, D53, K60, G61, K64, K65, E69, and/or E72 converted to alanine.
  • GST-OmpA mutants were expressed and purified as previously described (Ojogun et al, 2012).
  • Plasmids encoding His- tagged wild type and site-directed mutant OmpA proteins were generated by amplifying wild type and mutant ompA sequences using primers containing ligase-independent cloning (LIC) tails and annealing the amplicons into the pET46 Ek/LIC vector (Novagen, EMD Millipore, Darmstadt, DE) per the manufacturer's instructions. His-OmpA proteins were expressed and purified by immobilized metal-affinity chromatography as previously described (Miller et ai, 2011).
  • the mature OmpA sequence was threaded onto the solved crystal structures of proteins with similar sequences using the PHYRE2 server as previously described (Ojogun et ah, 2012).
  • Amino acids 19 to 150 (73% of the mature OmpA sequence) were modeled with greater than 90% confidence to known structures for similar proteins (Protein Data Bank [PDB] files 2aiz ⁇ Haemophilus influenzae OmpP6 peptidoglycan associated lipoprotein (PAL)], 4g4v [Acinetobacter baumanni PAL], 4b5c [Burkholderia pseudomallei PAL], 31dt [Legionella pneumophila OmpA], 2kgw [Mycobaterium tuberculosis OmpATb]).
  • PDB Protein Data Bank
  • the remainder of the protein lacked sufficient homology to any experimentally derived structure, but could be modeled using the Poing method (Kelley et ai, 2009), which was performed as part of the Phyre2 analyses.
  • the sLe x -PSGL-lpeptide (residues 61 to 77) and the sLe x glycan itself was extracted from the solved crystal structure of PSGL-1 (PDB 1G1 S) in PyMol and saved as an individual PDB file.
  • Open Babel software was used to convert PDB files to PDBQT (Protein Data Bank, Partial Charge and Atom Type) format in order to perform OmpAsLe" docking analysis.
  • AutoDock Tools software was used to generate the docking output files for both the OmpA protein structure and the sLe x ligand.
  • the search location for OmpA was generated in AutoDock Tools by setting a search grid that encompassed OmpA residues 19 to 74.
  • Molecular docking was performed using AutoDock Vina to identify potential points of interaction between OmpA and sLe x .
  • the top two OmpA-sLe models generated by AutoDock Vina had the same predicted affinity value of -4.2 kcal/mol and were selected for analysis in PyMol to determine potential points of contact.
  • Antibodies Antibodies, reagents, enzyme-linked immunosorbent assay (ELISA), and Western blotting
  • peptides corresponding to OmpA residues 23 to 40, 41 to 58, and 59 to 74 and Aspl4 residues 98 to 112 and 1 13 to 124 were synthesized, conjugated to keyhole limpet hemocyanin, administered to rabbits, and the resulting OmpA and Asp 14 peptide-specific sera were affinity-purified by New England Peptide (Gardner, MA).
  • the NCH-1 gene sequence for ompA was previously determined (Ojogun et al., 2012; Kahlon et al., 2013; Seidman et al., 2014).
  • a Protein BLAST (basic local alignment search tool) search using the NCH-1 OmpA predicted protein sequence as the query was used to identify homologs in other Anaplasmataceae species and in A. phagocytophilum strains HZ (Rikihisa et al, 1997), HGE1 (Goodman et al., 1996), Dog (Al-Khedery et al., 2012), JM
  • CRT35 and CRT38 are isolates of the A phagocytophilum 1 strain that were recovered from ticks collected at Camp Ripley, MN, USA.
  • NorV2 is a naturally occurring A. phagocytophilum isolate that was maintained in an experimentally infected lamb, exhibits reduced virulence in sheep, and differs in its 16S rRNA gene sequence when compared to other sheep isolates. OmpA sequence alignments were generated using Clustal W.
  • RF/6A or HL-60 cells were incubated with 4 ⁇ recombinant protein in culture media for 1 h in a 37°C incubator supplemented with 5% C02 and a humidified atmosphere.
  • the cells were fixed in 4% PFA in PBS for 1 h at room temperature followed by incubation with CSLEX1 , KM93, or G72 for 1 h at room temperature.
  • Antibody incubations and washes were performed as described previously (Reneer et al., 2006).
  • Spinning-disk confocal microscopy using an Olympus BX51 microscope affixed with a disk- spinning unit (Olympus, Center Valley, PA) and/or flow cytometry using a BD FACS CantoTM II (BD Biosciences) were performed to assess binding of antibodies or His-OmpA proteins to host cell surfaces as previously described (Ojogun et al, 2012; Kahlon et al, 2013).
  • RF/6A cells were pretreated with a2,3/6-sialidase, al,3/4-fucosidase, AAL, MAL II, or sLe x - or 6-sulfo-sLe x -specific antibodies prior to incubation with His-OmpA.
  • A. phagocytophilum DC bacteria were incubated with serially diluted concentrations of antiserum.
  • Preimmune rabbit serum 200 ⁇ g/mL was a negative control.
  • Assays using combinations of two or three different OmpA, Asp 14, or AipA peptide antibodies were performed using 100 ⁇ g/mL per antibody.
  • Preimmune serum 200 ⁇ g/mL or 300 ⁇ g/mL, based on the combined total of peptide antisera
  • Competitive inhibition assays using OmpA and/or Asp 14 Fab fragments were performed exactly as described for antisera.
  • Preimmune Fab fragments served as a negative control.
  • OmpA coated bead uptake assay 1.8 x 107 red fluorescent sulfate-modified 1.0 ⁇ diameter microfluorospheres (Life Technologies, Carlsbad, CA) were mixed by rotation with 8 ⁇ g of His-OmpA, or His-OmpA proteins bearing alanine substitutions, in 400 of 50 mM phosphate buffered saline (PBS) supplemented with 0.9% NaCl at room temperature overnight in the absence of light.
  • PBS phosphate buffered saline
  • the His- OmpA coated beads were centrifuged at 5,000 g for 25 min, followed by three washes in 50 mM PBS.
  • Coated beads were resuspended in 400 ⁇ , of 50 mM PBS, 0.9% NaCl, 1% BSA and stored at 4°C until use.
  • 1.8x104 of the beads were screened by immunofluorescent microscopy using mouse polyclonal OmpA antisera followed by Alexa Fluor 488-conjugated goat anti-mouse IgG as described (Ojogun et al., 2012).
  • His-OmpA coated beads or uncoated control beads were resuspended in the appropriate culture medium and added to host cells at a concentration of 500 beads/cell.
  • beads were centrifuged onto the host cells at 1,000 g for 5 min. The cells plus beads were incubated for 1 h at 37°C in a 5% C0 2 supplemented humidified incubator followed by washing the cells three times with PBS to remove unbound beads.
  • Non-adherent HL-60 cells were mixed with the beads in suspension, incubated as described above, and three PBS washes were performed intermittently between five-min spins performed at 300 g. To assess binding, the host cells were fixed in 4%
  • PFA paraformaldehyde
  • DAPI Pro Long® Antifade Gold gel mounting medium containing 4',6-diamidino-2-phenylindole
  • DAPI 4',6-diamidino-2-phenylindole
  • the host cells were resuspended in culture medium and cultivated for an additional 7 h. The cells were washed three times in PBS, incubated with a 0.25% trypsin solution
  • HL- 60 cells were cytospun onto glass microscope slides and fixed, mounted, and screened as described above.
  • RF/6A cells were added to wells containing coverslips, incubated overnight in a 37°C incubator supplemented with 5% CO? and a humidified atmosphere to allow the host cells to adhere prior to further processing.
  • His-OmpA coated bead binding or uptake was temperature sensitive, some experiments were performed at 4°C.
  • sLe x or PSGL-1 determinants were pretreated with a2,3-sialidase (5 ⁇ g/mL), al ,3/4-fucosidase ( ⁇ /mL), sLe x -specific antibody CSLEX1 (10 ⁇ ⁇ ,), PSGL-1 N-terminus-specific antibody KPL-1 (10 ⁇ g/mL), or vehicle or isotype controls as previously described (Ojogun et al., 2012) prior to the bead binding and uptake assays.
  • a2,3-sialidase 5 ⁇ g/mL
  • al ,3/4-fucosidase ⁇ /mL
  • sLe x -specific antibody CSLEX1 10 ⁇ ⁇
  • PSGL-1 N-terminus-specific antibody KPL-1 10 ⁇ g/mL
  • vehicle or isotype controls as previously described (Ojogun et al., 2012) prior to the bead binding and uptake assays.
  • Coverslips of RF/6A cells were incubated with OmpA coated or control beads as described above.
  • the coverslips were fixed in 2.0% glutaraldehyde in 0.1 M sodium cacodylate for 1 h at room temperature.
  • the coverslips were subjected to two 10-min washes in 0.1 M sodium cadodylate and fixed in 1.0% osmium tetroxide in 0.1 M sodium cacodylate for 1 h.
  • the coverslips were rinsed two more times with 0.1 M sodium cadodylate buffer for 10 min each.
  • the samples were dehydrated by successive 5 -min incubations in 50% ethanol, 70% ethanol, 80%) ethanol, 95% ethanol, and three 10-min washes in 10% ethanol.
  • the samples were incubated three times for 30 min each in hexamethyldisilazane, air-dried, mounted with silver paint, and sputter coated with gold before imaging on a Zeiss EVO® 50XVP scanning electron microscope (Thornwood, NY).
  • HL-60 cells incubated in suspension with beads, the samples were retained on a 0.1 ⁇ filter and processed exactly as described for RF/6A cells.
  • the Prism 5.0 software package 680 (Graphpad, San Diego, CA) was used to determine the statistical significance of data using one-way analysis of variance (ANOVA) or the Student's T-test, as previously described (Ojogun et al., 2012). Statistical significance was set to P ⁇ 0.05.
  • OmpA amino acids 59 to 74 are critical for A. phagocytophilum to bind to sLex capped PSGL-1 and for infection of mammalian host cells
  • the OmpA region that is important for A. phagocytophilum infection of mammalian host cells lies within residues 19 to 74 (OmpA19-74) (Ojogun et al., 2012).
  • polyclonal antisera against peptides corresponding to OmpA amino acids 23 to 40, 41 to 58, and 59 to 74 were raised. It was verified that the antisera were specific for OmpA by confirming that each recognized recombinant forms of mature OmpA (minus the signal sequence; corresponding to residues 19 to 205 and hereafter referred to as OmpA) and OmpA19-74, but neither OmpA75-205 nor Aspl4 (Fig. 8A).
  • Anti-OmpA41-58 and anti-OmpA59-74 were specific for their target peptides at all serum dilutions.
  • Anti-OmpA23-40 was specific for its target peptide at most dilutions tested, but exhibited low level recognition of OmpA41-58 at dilutions below 1 : 12,800 (Fig. 8A).
  • OmpA antibody reduced the percentage of A. phagocytophilum infected HL-60 cells by approximately 40% (Fig. 8B).
  • OmpA59-74 antibody exhibited a dose-dependent inhibitory effect and, at a concentration of 200 ug/ml, reduced the percentage of infected HL-60 cells by approximately three-fold.
  • Antisera targeting OmpA residues 23 to 40 and 41 to 58 exhibited very little to no inhibition of infection, regardless of concentration. Unless otherwise specified, all antisera were used at a concentration of 200 ug/ml in subsequent blocking experiments.
  • sLe x -capped PSGL-1 is an A. phagocytophilum receptor on human myeloid cells
  • the sLe x glycan (Fig. 9B) was extracted from the crystal structure of sLe x -capped PSGL-1 (DOI: 10.2210/pdblgls/PDB). Autodock Vina was used to predict how OmpA might interact with sLe ⁇
  • the search grid encapsulated OmpA19-74 (Fig. 9A).
  • K.64 of OmpA was predicted to bind the a2,3-sialic acid residue of sLe x (Fig. 9E and F).
  • G61 was also predicted to interact with sLe x in both models, though it was predicted to bind a2,3-sialic acid in one model and al,3-fucose in the other.
  • K60 was predicted to bind the Bl,3-galactose residue of sLe x in the docking model presented in Fig. 9F.
  • OmpA 9- 74 contains critical residues that interact with sLe x to promote A. phagocytophilum infection of host cells.
  • OmpA is conserved among A. phagocytophilum strains and K64 is conserved among Anaplasmataceae OmpA proteins
  • NCH-1 OmpA residues 19 to 74 were aligned with corresponding regions of OmpA homologs from marginale and Ehrlichia spp., which are in the family A naplasmataceae with A. phagocytophilum and infect bovine erythrocytes and human and animal monocytes, respectively (Carylon et al, 2012; Mansueto et al, 2012; Suarez et al., 201 1). A.
  • phagocytophilum OmpA K64 that was predicted to potentially interact with sLe x (Fig. 9, E and F), was the only binding domain residue that was conserved among all Anaplasmataceae OmpA regions examined (Fig. 10B). Additional residues within the A. phagocytophilum OmpA binding domain, including the other two predicted to interact with sLe , K60 and G61 (Fig. 9, E and F), were conserved among Anaplasma spp. but not Ehrlichia spp. OmpA proteins (Fig. 10B).
  • G61 and 64 are essential for recombinant OmpA to optimally bind to mammalian host cells and competitively inhibit A. phagocytophilum infection
  • OmpA Recombinant OmpA can be used as a competitive agonist to block A.
  • OmpA proteins N-terminally fused to glutathione-S-transferase (GST) were generated, each of which had an insertion of the peptide CLNHL at one of six different sites within residues 19 to 78.
  • phagocytophilum infection were identified.
  • the competitive agonist assay was repeated using GST-OmpA proteins in which select amino acids had been mutated to alanine (Fig. 10 and Fig. 1 1, B and C). Many of the targeted residues were within OmpA amino acids 59 to 74. R32 and D53 were selected because they lie outside of residues 59 to 74, and, accordingly, it was anticipated that substituting them would not alter OmpA function.
  • GST-OmpAK64A was considerably reduced in its ability to inhibit A. phagocytophilum infection (Fig. 1 1 , B and C), thereby indicating that this highly conserved residue was critical for GST-OmpA to serve as a competitive agonist.
  • GST-OmpAG61 A displayed a modest but significant decline in its competitive agonist ability. Replacement of both G61 and K64 with alanines yielded an additive effect that was greater than substituting either residue alone, as GST
  • OmpAGK6164AA was unable to inhibit infection.
  • GST-OmpA proteins in which R32, D53, K60, E69 and E72 had been mutated to alanine were each unaffected in the ability to hinder infection.
  • OmpAD53A bound equally well to RF/6A cells (Fig. 1 ID). His-OmpAG61A bound poorly, His- OmpAK64A even more so, and His-OmpAGK6164AA could not bind to host cells.
  • phagocytophilum to bind PSGL-l-modeled glycopeptides, to bind and invade human and murine myeloid cells, and to establish infection in laboratory mice. Consistent with these observations, PSGL-1 CHO cells that had been pretreated with al,3/4- fucosidase were approximately three-fold less permissive for A. phagocytophilum binding (Fig. 12A). Multiple lines of evidence led to the hypothesis that OmpA binds al,3-fucose. First, OmpA binds a2,3 -sialic acid, which is in close proximity to a 1,3 -fucose on sLe x . Second, the docking model in Fig.
  • OmpA residues within the binding domain contact both a2,3-sialic acid and al ,3-fucose of sLe x .
  • OmpA is important for A. phagocytophilum infection of not only myeloid, but also endothelial cells.
  • fucose residues are critical for the pathogen to invade RF/6A endothelial cells, as pretreatment of the host cells with al ,3/4- fucosidase made them significantly less permissive to A. phagocytophilum binding (Fig. 12B) and infection (Fig. 12C).
  • OmpA recognizes fucose
  • His-tagged OmpA was incubated with RF/6A cells that had been treated with al ,3/4-fucosidase and binding was assessed by
  • a2,3/6-sialidase-treated RF/6A cells were included as a positive control for a treatment that would make the host cells less permissive to recombinant OmpA binding.
  • treated and untreated host cells were screened with AAL (Aleuria aurantia lectin) and MAL II (Maackia amurensis lectin II).
  • AAL recognizes fucose residues that are in al,3- and l ,6-linkages with N- acetylglucosamine (Yamashita et al., 1985; Chandrasekaran et al., 2003).
  • MAL II detects sialic acids that are in a2,3-linkages with galactose. Fucosidase treatment abolished AAL but not MAL II binding, while sialidase treatment eliminated MAL II but not AAL binding (Fig. 13, A to D). Thus, the glycosidases were effective and specific. His-OmpA binding to both sialidase- and fucosidase-treated RF/6A cells was comparablyreduced relative to vehicle control treated cells, while binding of His-Aspl4 was unaffected. Incubating the host cells with MAL II or AAL prior to the addition of His-OmpA competitively reduced the efficiency of His-OmpA binding by similar degrees as sialidase or fucosidase, respectively (Fig.
  • OmpA interacts with 6-sulfo sLe* on RF/6A endothelial cell surfaces Because His-OmpA binding to RF/6A cells involved recognition of a2,3-sialic acid and al ,3-fucose (Fig. 13), it was hypothesized that OmpA interacts with sLe” or a sLe -like receptor on these host cells.
  • sLe x and the sLe x -like molecule, 6-sulfo sLe x (Neu5Ac(a2-3)Gal(pi- 4)[Fuc(al-3)][HS03(3-6)]GlcNAcl) (Fig.
  • OmpA-coated beads bind to and are internalized by non-phagocytic endothelial cells
  • phagocytophihim DC organism 0.8 ⁇ 0.2 ⁇ .
  • Successful conjugation of His-OmpA to the beads was confirmed by immunofluorescence using OmpA antiserum (Fig. 15A).
  • RF/6A cells were incubated with recombinant OmpA-coated or non-coated control beads and screened with OmpA antibody to determine the numbers of beads bound per cell. To assess bead uptake, the cells were incubated for an additional 1 to 8 h and trypsin was used to remove non-internalized beads prior to screening. Immunofluorescence microscopy revealed that significantly more OmpA coated beads bound to and were internalized by RF/6A cells versus control beads (Fig. 15, B and D).
  • OmpA K6465AA were significantly compromised in their abilities to bind to and be
  • Asp 14 is a 124-amino acid (13.8 kDa) protein, and its binding domain lies within residues 101 to 124.
  • antisera were raised against residues 98 to 1 12 and 1 13 to 124. Both antisera recognized GST-Aspl4, but not GST-Aspl41-88 or GST alone.
  • antiserum targeting Aspl 498-112 but not Aspl41 13-124 detected GST- Aspl 41-112 and each antiserum was specific for the peptide against which it had been raised.
  • Fab fragment fragment antigen binding portions of OmpA23-40, OmpA41-48, OmpA59-74, Aspl498-112, Asp 141 13-124, or OmpA59-74 and Aspl41 13-124 antibodies were prepared and assessed for the ability to inhibit ⁇ , phagocytophilum infection of HL-60 cells. Consistent with results obtained using intact antibodies, OmpA59-74 Fab, Aspl4113-124 Fab, and the combination thereof achieved the greatest reductions in the percentage of infected cells and morulae per cell (Fig. 17, C and D).
  • Example 1 It was shown in Example 1 that a combination of antisera that had been raised against the entireties of OmpA, AipA, and Asp 14 strongly inhibited A. phagocytophilum infection of mammalian host cells.
  • DC organisms were treated with a cocktail of antibodies specific for OmpA59-74, Aspl41 13-124, and AipA9-21 prior to incubating the bacteria with HL-60 cells. This antibody combination significantly attenuated infection, reducing the percentage of infected cells and number of morulae per cell by approximately five- fold (Fig. 18, A and B).
  • the reduction in infection achieved using the combination antisera was due to effective blocking of bacterial adhesion to HL-60 cell surfaces, as combination antisera specific for OmpA59-74, Aspl4113-124, and AipA9-21 reduced the numbers of bound A. phagocytophilum organisms per cell by more than four-fold relative to the same amount of preimmune serum (Fig. 18C).
  • the observed reductions in bacterial adhesion and infection achieved by targeting all three binding domains were greater than those achieved using (1) antibodies that targeted only one or two of the binding domains and (2) combinations of antibodies against one or two of the binding domains together with antibodies against irrelevant portions of OmpA, Asp 14, or AipA.
  • targeting the OmpA, Asp 14, and AipA binding domains together produced a synergistic blocking effect that protects host cells from A.
  • the OmpA binding domain lies within amino acids 59 to 74 and it, like the rest of the protein, is highly conserved among A. phagocytophilum strains known to cause disease in humans and animals.
  • Antibody against OmpA59-74 inhibited bacterial binding to PSGL-1 CHO cells and infection of HL-60 cells.
  • OmpA59-74 is predicted to be a solvent exposed alpha helix and part of a cationic surface patch that binds sLe x , an interaction that is similar to those between staphylococcal superantigen-like (SSL) protein family members and sLe ⁇ SSL4, SSL5, and SSL1 1 each use basic residues within cationic surface pockets to interact with a2,3-sialic acid of sLe x (Chung et al., 2007; Hermans et al., 2012; Baker et al, 2007).
  • SSL staphylococcal superantigen-like
  • pathogens' sialic acid binding proteins including uropathogenic Escherichia coli sialic acid-specific S fimbrial adhesin (Morschhauser et al., 1990), pertussis toxin of Bordetella pertussis (Stein et al., 1994), influenza viral neuraminidase (Varghese et al., 1992), canine adenovirus 2 capsid protein (Rademacher et al., 2012), and rhesus rotavirus VP4 (Dormitzer et al, 2002) all use basic residues localized within cationic surface pockets to target sialic acid.
  • the Asp 14 binding domain is within amino acids 1 13 to 124.
  • Antibody specific for Aspl41 13-124 abrogated bacterial binding and infection of host cells.
  • Aspl4 bears no semblance to any known crystal structure, it could not be modeled. However, from the data presented herein it can be inferred that Asp 14 amino acids 1 13 to 124 are exposed on the surfaces of A. phagocytophilum and the invasin itself.
  • OmpA K64 is essential for and G61 contributes to the ability of OmpA to bind to mammalian host cells.
  • These experimental findings support the top two OmpA-sLe" docking models, both of which predicted the involvement of K64 and G61 in interacting with a2,3-sialic acid and al ,3-fucose of sLe ⁇
  • the actual interactions between OmpA and sLe x are likely a hybrid of those predicted by the two docking models because, while both predicted the involvement of K64 and G61, one also predicted the involvement of K60, which was found to be negligible for OmpA to act as a competitive agonist.
  • OmpA K64 and G61 may play functionally conserved roles among members of the fdmWyAnaplasmataceae and the genus Anaplasma.
  • K64 is present in all Anaplasma and Ehrlichia spp. OmpA proteins, while G61 is conserved among Anaplasma but not Ehrlichia spp. OmpA proteins.
  • E. chaffeensis OmpA contributes to infection of monocytic cells (Cheng et al., 2011). Compared to the conservation exhibited among Anaplasma spp. OmpA proteins, A.
  • phagocytophilum and E. chaffeensis OmpA proteins are more divergent in sequence, especially in the binding domain, which may contribute to these pathogens' tropisms for different leukocytes. Still, because of its conservation, the E. chaffeensis OmpA residue that corresponds to A. phagocytophilumOmpA K64 may be involved in binding to a sLe x -related glycan on monocytic cells.
  • al,3-fucose is critical for A. phagocytophilum binding and infection.
  • OmpA binds l ,3-fucose, as can be inferred from the observations that recombinant OmpA bound poorly to RF/6A endothelial cells from which l ,3/4-fucose residues had been removed or that had been incubated with the al ,3/6-fucose-specific lectin, AAL.
  • OmpA ability of OmpA to bind a2,3-sialic acid and al,3-fucose is consistent with the close proximity of the two sugar residues to each other in sLe x and related glycans and also with OmpA-sLe x molecular docking predictions.
  • RF/6A cells which support ⁇ , phagocytophilum binding and infection, express very little to no sLe x . Rather, they express 6-sulfo-sLe x , which presents a2,3 -sialic acid and l ,3-fucose in the same orientation and proximity to each other as sLe x .
  • A. phagocytophilum OmpA interacts with glycans that present a2,3-sialic acid and ccl,3-fucose in a similar manner as sLe ⁇
  • OmpA by itself functions as both an adhesin and an invasin, as demonstrated by the ability of His-OmpA to confer adhesive and internalization capabilities to inert beads.
  • the Sca2 autotransporter protein from Rickettsia conorii is sufficient to mediate adherence to and invasion of cultured mammalian cells.
  • Murine neutrophils require alphal ,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. Blood 102, 3387-3395.
  • Rickettsial outer-membrane protein B mediates bacterial invasion through u70 in an actin, c-Cbl, clathrin and caveolin 2-dependent manner.
  • influenzae vaccine candidate outer membrane protein P6 is not conserved in all strains.
  • the rhesus rotavirus VP4 sialic acid binding domain has a galectin fold with a novel carbohydrate binding site.
  • Lcl of Legionella pneumophila is an immunogenic GAG binding adhesin that promotes interactions with lung epithelial cells and plays a crucial role in biofilm formation.
  • Legionella pneumophila mediates invasion in a HeLa cell model. Infection and immunity 66, 4602-4610.
  • Leukocyte infection by the granulocytic ehrlichiosis agent is linked to expression of a selectin ligand.
  • TMbase- A database of membrane spanning proteins
  • GTPases that are predominantly associated with recycling endosomes. Cellular microbiology 12, 1292-1307.
  • Anaplasma phagocytophilum APH_0032 is expressed late during infection and localizes to the pathogen-occupied vacuolar membrane. Microbial pathogenesis 49, 273-284.
  • Haemophilus influenzae protein F mediates binding to laminin and human pulmonary epithelial cells. The Journal of infectious diseases 207, 803-813.
  • PilA protein of non-typeable Haemophilus influenzae plays a role in biofilm formation, adherence to epithelial cells and colonization of the mammalian upper respiratory tract. Molecular microbiology 65, 1288-1299.
  • Anaplasma phagocytophilum Asp 14 is an invasin that interacts with mammalian host cells via its C terminus to facilitate infection. Infection and immunity 81, 65-79.
  • phagocytophilum infects mast cells via alphal,3-fucosylated but not sialylated glycans and inhibits IgE-mediated cytokine production and histamine release.
  • Anaplasma phagocytophilum outer membrane protein A interacts with sialylated glycoproteins to promote infection of mammalian host cells. Infection and immunity 80, 3748-3760.
  • phagocytophilum PSGL-1 -independent infection does not require Syk and leads to less efficient AnkA delivery.
  • the Rickettsia conorii autotransporter protein Seal promotes adherence to nonphagocytic mammalian cells.
  • phagocytophilum surface protein AipA mediates invasion of mammalian host cells.
  • pneumophila collagen-like protein encoded by a gene with a variable number of tandem repeats is involved in the adherence and invasion of host cells.
  • Varghese JN McKimm-Breschkin JL, Caldwell JB, Kortt AA, Colman PM (1992) The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor. Proteins 14: 327-332.
  • Leptospiral endostatin-like protein A is a bacterial cell surface receptor for human plasminogen. Infection and immunity 78, 2053-2059.

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Abstract

Anaplasma phagocytophilum surface protein AipA and/or fragments thereof which comprise an invasin domain are used in compositions suitable to elicit an immune response to treat or prevent infections caused by tick-born bacteria of the Anaplasmatacaea family. AipA proteins and protein fragments or antibodies directed to AipA proteins and protein fragments are also used in diagnostic assays to detect exposure to and/or infection with Anaplasmatacaea. AipA and/or fragments thereof are also used for these purposes in combination with one or both of Asp14 and OmpA proteins and/or fragments thereof which comprise an invasin domain. Homologs of these proteins are also used in the compositions and assays.

Description

AIPA, OMPA, AND ASP14 IN VACCINE COMPOSITIONS AND DIAGNOSTIC TARGETS FOR ANAPLASMA PHAGOCYTOPHILUM INFECTION
FIELD OF THE INVENTION
The invention generally relates to a vaccine or immunogen and diagnostic for anaplasmosis in mammals. In particular, the invention provides Anaplasma phagocytophilum (AipA) protein and fragments thereof comprising an invasin domain for these purposes, used alone or in combination with one or both of Anaplasma phagocytophilum outer surface protein A (OmpA) and/or antigenic fragments thereof, and Anaplasma phagocytophilum surface protein 14 (Asp 14) and/or antigenic fragments thereof.
PRIORITY
This application claims the benefit of U.S. application 61/935,012, filed February 3, 2014. This application is incorporated herein by reference in its entirety.
SEQUENCE LISTING
This document incorporates by reference an electronic sequence listing text file, which was electronically submitted along with this document. The text file is named
02940981TA_ST25.txt, is 108 kilobytes, and was created on January 30, 2015.
BACKGROUND OF THE INVENTION
Anaplasma phagocytophilum {Aph) is a tick-transmitted obligate intracellular bacterium of the family Anap I as mataceae that can infect humans, livestock, companion animals, and wild animals. In addition to Aph, the Anaplasmataceae family members include Anaplasma marginale, Anaplasma platys, Ehrlichia chaffeensis, Ehrlichia canis, and Ehrlichia ruminatium, among others, and all of these cause similar infections known collectively as ehrlichiosis or anaplasmosis. When humans contract an Aph infection, it is more specifically known as human granulocytic anaplasmosis (HGA) and is marked by fever and increased susceptibility to potentially fatal opportunistic infections. Other possible disease manifestations include leukopenia, thrombocytopenia, and elevated serum transaminase levels. Unfortunately, no vaccines against these infectious agents are currently available.
Instead, antibiotic treatments with doxycycline or tetracycline are currently the frontline treatments for anaplasmosis/ehrlichiosis and can be effective. However, the overuse of antibiotics is currently of great concern, and many individuals, such as those that are allergic to the drug, pregnant women, and small children, either cannot or are not advised to take doxcycline. Also troubling is the lack of tools for rapid and definitive diagnosis of
anaplasmosis/ehrlichiosis in any species other than dogs.
HGA is an emerging and potentially fatal disease transmitted by the same vectors that transmit Lyme Disease, primarily ticks and deer, but other animal and human hosts can complete the vector cycle and therefore extend the spread of disease. In the U.S., HGA occurs primarily in the Northeast, Upper Midwest, and Northern California. Since HGA became a reportable disease in the U.S. in 1999, the number of cases has risen annually, reaching 2,037 in 2013. Since diagnostic tools for HGA are lacking, the number of actual cases is likely to be much higher. HGA is increasingly recognized in Europe and Asia, and Aph infection is now the most widespread tick-transmitted disease of animals in Europe. Domestic animals, such as dogs and cats, and livestock, such as sheep can be become infected.
As the name implies, an obligate intracellular bacterium must enter a target cell in its human or animal host to survive, replicate, and move to the next host. When an Anaplasma spp. or Ehrlichia spp. infected tick bites a subject, the bacteria is transferred from the tick salivary glands into the tissues of the host or into the bloodstream where they bind to the surface of host cells (especially neutrophils) and are internalized by being taken up into vacuoles that form around each bacterium. For A. phagocytophilum, optimal invasion of mammalian host cells is known to involve multiple cell surface proteins (various adhesins and invasins) which function cooperatively. Within the cell, a resident bacterium prevents the vacuole from merging with lysosomes, which would otherwise destroy the bacterium. In doing so, the bacterium converts the cell into a protective niche that favors bacterial survival and completion of its zoonotic cycle. For example, A. phagocytophilum undergoes a biphasic developmental cycle within neutrophils in which it transitions between an infectious morphotype (dense-cored cell; DC) and a noninfectious, replicative morphotype (reticulate cell; RC). Subsequently, if the Aph infected host is bitten by a tick, DC cells from the host can be ingested by the tick during the blood meal, and the disease is then transferred to new hosts when the infected tick takes another blood meal. However, HGA can also be transmitted perinatally and by blood transfusion, and possibly nosocomially.
While the hallmark of Aph infection is Aph colonization of neutrophils, Aph has also been detected in the microvascular endothelium of heart and liver in experimentally infected severe combined immunodeficiency mice. For experimental purposes, promyelocytic and endothelial cell lines are also useful as in vitro models for studying Aph-host cell interactions. It has been shown that when na'ive neutrophils or HL-60 cells are overlaid on 4p/i-infected endothelial cells, the bacterium rapidly transmigrates into the myeloid cells, demonstrating that Aph infects endothelial cells in vitro and suggesting that the bacterium may transmigrate between endothelial cells and neutrophils during the course of mammalian in vivo infections.
As noted above, humans are not the only species that are infected by members of the Anaplasmataceae family. For example, A. marginale infects bovine erythrocytes. Bovine anaplasmosis causes severe economic loss in many countries, mainly due to the high morbidity and mortality in susceptible cattle herds. Several parameters contribute to the losses due to anaplasmosis and include: low weight gain, reduction in milk production, abortion, the cost of anaplasmosis treatments, and mortality. The losses incurred by the U.S. cattle industry as a result of anaplasmosis are estimated to be $300 million per year whereas in Latin America, losses are calculated to be approximately $800 million per year.
Proteins which mediate the infection of endothelial cells and/or the dissemination of bacteria into the microvasculature of heart and liver are attractive targets for preventing disease caused by Anaplasmataceae bacteria such as Aph. In particular, targeting ^p/z proteins that are conserved among related Anaplasmataceae family members may also reduce or block
transmission of disease caused by a broad spectrum of Anaplasmataceae species. In addition, it would be advantageous to have available a rapid and highly accurate diagnostic test for detecting Anaplasmataceae species infection, especially one that allowed detection early in infection. Unfortunately, no FDA-approved vaccines against Anaplasmataceae species having purified antigens are currently available.
The current gold standard serologic test for diagnosis of anaplasmosis in humans is indirect immunofluorescence assays (IF A) performed as timed pairs over a period of a few weeks, only available in specialized reference laboratories. This assay measures non-specific increases in IgM and IgG antibody levels. However, IgM antibodies, which usually rise at the same time as IgG near the end of the first week of illness and remain elevated for months or longer, are even less specific than IgG antibodies and more likely to result in a false
positive. Serologic tests based on enzyme immunoassay (EIA) technology are available from some commercial laboratories. However, EIA tests are qualitative rather than quantitative, meaning they only provide a positive/negative result, and are less useful to measure changes in antibody titers between paired specimens. Furthermore, some EIA assays rely on the evaluation of IgM antibody alone, which again may have a higher frequency of false positive results.
Between 5-10% of currently healthy people in some areas may have elevated antibody titers due to past exposure to Aph or to other Anaplasmataceae family members. If only one sample is tested, it can be difficult to interpret. A four- fold rise in antibody titer is needed to achieve significance in paired samples taken weeks apart. Thus, tools for a rapid and definitive diagnosis in any species other than dogs are lacking.
US 7,906,296 B2 to Beall et al teaches polynucleotide sequences from major outer surface protein P44 of Anaplasma platys (Apl), which causes tick-born anaplasmosis in dogs. P44 and peptides from the translated protein can be used for detection of Apl and Aph infection and/or to elicit an immune response in vivo and confer resistance to anaplasmosis caused by Apl ox Aph.
US 8,158,370; 8,303,959 and US 2013/0064842, all to Liu et al, teach the use of P44 surface proteins from various strain variants of Aph to diagnose and protect against
anaplasmosis.
US 8,609,350 B2 to Liu et al. teaches polypeptides ixom Aph to diagnose and protect against anaplasmosis. The Aph sequences were derived from APH_0915, which encodes a hypothetical open reading frame of a protein of unknown function.
PCT/US2013/047325 to Carlyon, which is herein incorporated by reference, relates to the invasin proteins OmpA and Asp 14, and fragments thereof comprising conserved invasin domains. The proteins and fragments are used as therapeutic and diagnostic agents for phagocytophilum infection.
Nelson, CM., et al. {BMC Genomics 9, 364; 2008) used a whole genome transcriptional profiling tiling array analysis to detect A. phagocytophilum genes that are upregulated in vitro during infection of mammalian versus tick cell lines. Many of the proteins encoded by the identified genes were assigned a designation of "hypothetical protein" and of these, several had a predicted cellular location of "outer membrane", among them APH0915. However, no further information about this putative protein was provided, and no confirmation of its status as an outer membrane protein or possible significance was shown.
A need remains in the art for immunogenic compositions and vaccines to combat Anaplasmataceae infections and for methods to rapidly and accurately diagnose new cases of these diseases, especially with respect to Anaplasmataceae infections that cause anaplasmosis and HGA.
SUMMARY OF THE INVENTION
Obligate intracellular bacteria such as Anaplasmataceae use outer membrane proteins called invasins to enter and infect eukaryotic host cells. Since these organisms are incapable of extracellular survival, blocking this internalization step prevents infection and transmission of diseases caused by Anaplasmataceae. Thus, the identification and characterization of
Anaplasmataceae invasin proteins provides a path forward for the development of therapies to treat and/or prevent Anaplasmataceae infections. In addition, the discovery and characterization of such invasins also leads to improved diagnostic methods for detecting exposure to and/or infection with Anaplasmataceae, so that proper therapeutic measures can be undertaken.
Provided herein is a newly characterized invasin protein from A. phagocytophilum, denoted AipA (Anaplasma phagocytophilum invasion protein A) (SEQ ID NO: 13). This protein corresponds to protein APH0915 identified by Nelson et al. (2008). The studies presented in Example 1 below demonstrate that AipA is in fact an outer membrane protein, and further that AipA is, surprisingly, an A. phagocytophilum invasin protein that is useful as a therapeutic and diagnostic agent. In addition, the detailed characterization of AipA presented herein has identified particular fragments of AipA which constitute domains required for the protein to function as an invasion, so-called effector or invasin domains, that are also useful as therapeutic and diagnostic agents. Further, AipA is unique to A. phagocytophilum i.e. homologs of AipA are not present in other Anaplasmataceae species. Accordingly, certain aspects of this disclosure provide AipA and/or one or more functional fragments thereof for use as components of vaccines and/or immunogenic compositions to prevent or treat A. phagocytophilum infections. Other aspects provide AipA protein and fragments thereof comprising invasin domains, and antibodies thereto, as diagnostic targets to detect exposure to and/or infection with A. phagocytophilum. Exemplary functional domains of AipA include but are not limited to fragments which include a linear amino acid sequence encompassing amino acids 1 -87 inclusive, and amino acids 9-21 (SEQ ID NO: 13), inclusive.
In other aspects, AipA and/or one or more of the identified functional fragments thereof are used for these purposes in combination with one or more additional invasins and/or functional domains of the additional invasins. Exemplary additional invasins include but are not limited to Aspl4 (14-kDa phagocytophilum surface protein; APH0248) and OmpA (Outer membrane protein A; APH0338 in the annotated A. phagocytophilum proteome), and homologs thereof from other species, e.g. other Anaplasmataceae species. Exemplary functional domains of Asp 14 include but are not limited to fragments which include the linear amino acid sequence from amino acids: 19-60, 101-1 12, 101-124, and 1 13-124, all of which are inclusive. In some aspects, the sequence is that which encompasses amino acids 1 13-124. Exemplary functional domains of OmpA include but are not limited to fragments which include the amino acid sequence from amino acids: 19-74, 59-74, 48-56, 86-101 , 135-155, and 24-121 , all of which are inclusive. In some aspects, the sequence is that which encompasses amino acids 59-74.
Accordingly, in some aspects, what is provided herein are various combinations of these three A. phagocytophilum surface proteins and/or invasion domain-containing fragments thereof, as well as antibodies to one or more of these proteins or fragments, as protective/therapeutic/diagnostic agents or targets to treat or detect Anaplasmataceae infection.
Significantly, it has been discovered that these three invasins work together
synergistically to promote infection of host cells, and that combinations of two of AipA, Asp 14 and OmpA proteins (or peptides comprising invasin domains thereof) promote infection more effectively than any one alone, and, in some aspects, the most effective combination utilizes all three. Conversely, to prevent infection of host cells from infection, some aspects of the invention provide therapeutic agents which are or which are based on combinations of at least two and usually all three of the proteins or relevant fragments thereof. For example, linear peptide domains of each protein that mediate invasin and combinations of linear peptide domains, are encompassed, as are antibodies targeting one, two, or all three domains. Such agents are shown herein to simultaneously work synergistically to nearly abolish A. phagocytophilum invasion of host cells. For example, antisera combinations targeting two of the three invasins together more effectively inhibited infection than an antiserum targeting an individual protein, and the most effective blocking of A. phagocytophilum infection was achieved using antisera against all three invasins.
An embodiment of the invention is a composition comprising one or more polypeptides which include one copy or more than one copy of SEQ ID NO: 14 together with one copy or more than one copy of either or both of SEQ ID NO: 3 and SEQ ID NO: 06. For example, a single polypeptide might include 1 , 2, or 3 copies of SEQ ID NO: 14 together with 0, 1, 2 or 3 copies of SEQ ID NO. 3 and/or 0, 1 , 2 or 3 copies of SEQ ID NO. 6, and may include additional amino acid sequences of interest (e.g. linkers, etc.). Or the composition may include two or more polypeptides wherein one polypeptide is or includes one or more copies of SEQ ID No. 14, and the other polypeptide(s) is/are or include(s) one or more copies of either or both of SEQ ID No. 3 and SEQ ID No. 6.
In exemplary embodiments, the isolated peptides are in a vehicle or carrier suitable for administration to a subject. At least one of the polypeptides may be linked to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, or one or more additional polypeptides comprising SEQ ID NO:01 , 02, 03, 04, 05, 06, 13, 14, or 15 or a combination thereof. An exemplary embodiment is one or more polypeptides linked to a protein purification ligand, and protein purification ligands are a peptide encoding a histidine tag.
Another embodiment of the invention comprises one or more polypeptides selected from the group consisting of SEQ ID NO:01, SEQ ID NO:02, and SEQ ID NO:03. Another embodiment of the invention comprises one or more polypeptides selected from the group consisting of SEQ ID NO:04, SEQ ID NO:05, and SEQ ID NO:06. Another embodiment of the invention comprises one or more polypeptides selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14, and SEQ ID NO: 15.
Another embodiment of the invention includes nucleic acids and nucleic acid based compositions that can be used for generating an immune response. In particular embodiments, the compositions may take the form of DNA vaccines or other nucleic acid vaccines which comprise nucleic acids encoding one or more polypeptides as described herein, e.g. polypeptides which include one or more copies of SEQ ID NO: 14, optionally together with one or more copies of either or both SEQ ID NO: 3 and SEQ ID NO. 06. For example, a suitable composition might include a single nucleic acid in a vector (e.g., adenovirus, baculovirus, etc.) that codes for a single polypeptide or multiple polypeptides that include 1 , 2, or 3 copies of SEQ ID NO: 14, together with 0, 1, 2 or 3 copies of SEQ ID NO. 3; and 0, 1, 2 or 3 copies of SEQ ID NO. 6, and may include additional amino acid sequences of interest, linkers, etc. Or the composition may include two or more nucleic acids, each on a vector, which code for a polypeptide that is or includes one or more repeats of SEQ ID NO. 14, and code for another polypeptide is or includes one or more repeats of either or both SEQ ID NO. 3 and SEQ ID NO. 6. DNA may also be provided without a vector and be combined with material which might also be driven into a cell or living tissue by an agent which crosses into a cell (e.g., Lipofectamine). Furthermore, the DNA vaccines might be incorporated into a host bacterium that is itself administered as a vaccine to a subject.
Another embodiment of the invention is a method of protecting a subject from acquiring a zoonotic disease and/or treating a zoonotic disease in a subject by the step of administering a composition an immunogenic composition comprising the isolated polypeptide represented by SEQ ID NO: 14 and at least one of the isolated polypeptides represented by SEQ ID NO:03 and SEQ ID NO:06. At least one of the polypeptides may be linked to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, or one or more additional polypeptides comprising SEQ ID NO:01, 02, 03, 04, 05, 06, 13, 14, or 15; or a combination thereof. The zoonotic disease may be one caused by an obligate intracellular Anaplasmataceae bacterium selected from the group consisting of Anaplasma phagocytophilum, Anaplasma marginale,
Anaplasma platys, Ehrlichia chaffeensis, Ehrlichia canis, Ehrlicia ruminantium, and Ehrlichia ewingii. When the subject is a human, the zoonotic disease may be human granulocytic anaplasmosis (HGA) if caused by Anaplasma phagocytophilum. When the subject is an animal, the zoonotic disease may be anaplasmosis. If caused by an ehrlichia species, the human zoonotice disease may be monocytic ehrlichiosis or ehrlichiosis.
Another embodiment of the invention is a method of detecting antibodies that specifically bind an Anaplasmataceae polypeptide in a test sample. The method may include the steps of contacting a test sample, under conditions that allow polypeptide-antibody complexes to form, with a composition that includes at least one or more polypeptides encoding all or a portion of SEQ ID NO:01, SEQ ID NO:04, and/or SEQ ID NO: 13, and detecting said polypeptide-antibody complexes, wherein the detection is an indication that antibodies specific for Anaplasmataceae Asp 14, OmpA, or AipA are present in the test sample. Alternatively, antibodies are generated to at least one or more polypeptides encoding all or a portion of SEQ ID NO:01, SEQ ID NO:04, and/or SEQ ID NO:13, and the antibodies are used to detect the presence of AipA and/or OmpA and/or Asp 14 protein in a biological sample from a subject. The method may be an assay selected from the group consisting of an immunoblot and an enzyme-linked immunosorbent assay (ELISA). An exemplary embodiment of this methodology includes using at least one polypeptide which is or includes SEQ ID NO:03, SEQ ID NO:06, or SEQ ID NO: 14 in the assay, whereby infection with obligate intracellular Anaplasmataceae is determined from a serum sample exhibiting antibody binding with the at least one polypeptide.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 A-B. Schematic diagrams of A. phagocytophilum AipA membrane topology and sequence. (A) Diagram of the predicted topology of AipA in the A, phagocytophilum outer membrane. N, AipA amino terminus. C, AipA carboxy terminus. Numerical values indicate amino acid coordinates for predicted transmembrane spanning regions. (B) Diagrams of the AipA sequence. The scale indicates 50-amino acid intervals. For the Hydrophobicity diagram, the Kyte-Doolitle algorithm was used to determine hydrophobic (histogram above the axis) and hydrophilic (histogram below axis) regions. For the Surface diagram, the Emini algorithm was used to determine regions that are likely accessible on the surface of the AipA (histogram above the axis) or not (histogram below the axis). The AipA amino acid segments against which antisera were raised are indicated on the Hydrophobicity plot by horizontal lines.
Figure 2 A-F. Differential expression profiling of AipA throughout the A.
phagocytophilum life cycle. (A) aipA transcriptional profile during A. phagocytophilum infection of HL-60 cells. DC bacteria were incubated with HL-60 cells to establish a
synchronous infection. Total NA isolated from the DC inoculum and infected host cells at several postinfection time points was subjected to reverse transcriptase-quantitative PCR (RT- qPCR). Relative aipA transcript levels were normalized to A. phagocytophilum 16s rRNA gene transcript levels. To determine relative aipA transcription between RC and DC organisms, normalized aipA transcript levels per time point were calculated as the fold change in expression relative to expression at 16 h, a time point at which the entire bacterial population is in the DC morphotype. Data are the means + standard deviations (SD) for triplicate samples and are representative of two experiments having similar results. (B) Western blot screening of whole- cell lysates of uninfected (U) and A. phagocytophilum infected HL-60 cells (I) using mouse antiserum raised against GST-AipAi-87 (aAipA1 -87) and GST-AipA24g-355 (aAipA249-355). (C) AipA expression over the course of infection of mammalian host cells. RF/6A cells that had been synchronously infected with A. phagocytophilum (Ap) were screened with antibodies targeting Msp2 (P44) (to denote all A. phagocytophilum inclusions) and AipA viewed by confocal microscopy. Data presented are the mean percentages + SD of Msp2 (P44)-positive A.
phagocytophilum inclusions that were also AipA-positive. At least 100 bacterial inclusions were scored per time point. (D) aipA and groEL expression during transmission feeding of A.
phagocytophilum infected ticks on naive mice. A. phagocytophilum-mfected I. scapularis nymphs were allowed to feed on mice for 72 h. Total RNA recovered from unfed and transmission-fed ticks that had been removed at 24, 48, and 72 h postattachment was subjected to RT-qPCR. Relative aipA and groEL transcript levels were normalized to A. phagocytophilum 16S rRNA gene levels. (E) AipA is not expressed during A. phagocytophilum infection of a tick cell line. Western blot analysis of uninfected and A. phagocytophilum infected (Inf.) HL-60 and ISE6 cells using antiserum specific for AipAi-87 or APH0032. The number of weeks (Wk.) during which A. phagocytophilum was maintained in ISE6 cells are indicated. (F) AipA is expressed in vivo and elicits a humoral immune response. Western blot analysis of GST and GST-AipAi-87 screened with sera from an HGA patient and from an A. phagocytophilum infected dog. Results presented in panels B to F are each representative of at two to three independent experiments with similar results. Statistically significant (*, P < 0.05; **, P < 0.005; ***, P < 0.001) values are indicated.
Figure 3A-B. AipA is an A. phagocytophilum OMP. (A) AipA colocalizes with the confirmed OMP, Msp2 (P44). A. phagocytophilum-infected RF/6A cells were fixed and viewed by confocal microscopy to assess immunoreactivity with AipA antiserum in conjunction with Msp2 (P44) antiserum. Host cell nuclei were stained with DAPI (4',6'-diamidino-2- phenylindole). The insets demarcated by solid boxes in the lower right corners of each panel are magnified versions of the representative A. phagocytophil m-occwpied vacuole that is denoted by the hatched box in each panel. (B) AipA is exposed on the bacterial surface. Intact A.
phagocytophilum DC organisms were incubated with trypsin or vehicle control, solubilized, and Western-blotted. Immunoblots were screened with antiserum targeting AipAi-87, Asp55, or APH0032. Data are representative of two experiments with similar results.
Figure 4A-F. GST-AipA requires amino acids 1 to 87 to bind and competitively inhibit phagocytophilum infection of mammalian host cells. (A and B) GST-AipAi-87 binds to mammalian host cells. RF/6A cells were incubated with GST-AipAi-87, GST-AipA249-355, or GST alone. (A) The host cells were fixed, screened with GST antibody, and examined using confocal microscopy. Host cell nuclei were stained with DAPI. Representative merged fluorescent images from three experiments with similar results are shown. (B) Flow cytometric analysis of GST fusion protein binding to RF/6A cells. (C to F) GST-AipAi-87 competitively inhibits A. phagocytophilum infection. HL-60 (C and D) and RF/6A cells (E and F) were incubated with DC bacteria in the presence of GST, GST-AipAi- 7, or GST-AipA24g_355 for 1 h. Following removal of unbound bacteria, host cells were incubated for 24 h (C and D) or 48 h (E and F) and subsequently examined using confocal microscopy to assess the percentage of infected cells (C and E) or the mean number (+ SD) of pathogen-occupied vacuoles per cell (D and F). Results shown are relative to GST-treated host cells and are the means + SD for three experiments. Statistically significant (*, P < 0.05; **, P < 0.005; ***, P < 0.001) values are indicated.
Figure 5A-C. Pretreatment of A. phagocytophilum with AipAi-87 antiserum inhibits infection of HL-60 cells but does not alter binding to sLex-capped PSGL-1. A.
phagocytophilum DC organisms were exposed to antiserum targeting AipAi-87, AipA249-355, OmpA, or preimmune serum and then incubated with HL-60 (A and B), PSGL-1 CHO cells, or untransfected CHO cells (C). The infection of HL-60 cells was allowed to proceed for 24 h prior to being assessed, while bacterial binding to PSGL-1 CHO cells was assessed immediately. The mean + standard deviations of percentages of infected HL-60 cells (A), A. phagocytophilum (Ap) vacuolar inclusions per HL-60 cell (B), and bound DC organisms per PSGL-1 CHO cell or untransfected CHO cell (C) were determined using immunofluorescence microscopy. Additional positive controls for blocking ^, phagocytophilum to PSGL-1 CHO cells, besides incubating bacteria with OmpA antiserum, were PSGL-1 CHO cells that had been incubated with PSGL-1 N-terminus blocking antibody KPL-1 or sLex-blocking antibody CSLEXl prior to the addition of bacteria. Negative controls were PGSL-1 CHO cells that had been incubated with isotype control antibodies prior to the addition of bacteria. Results shown are relative to GST-treated host cells and are the means + SD for three experiments. Statistically significant (***, P < 0.001) values are indicated.
Figure 6A-B. A combination of antisera targeting AipA, OmpA, and Aspl4 blocks A. phagocytophilum infection of host cells. DC bacteria were incubated with preimmune serum or antiserum targeting AipAi-87, OmpA, and/or Aspl4 and then incubated with HL-60 cells. (A) The cells were fixed and screened by confocal microscopy to assess the percentage of infected cells. Results shown are relative to host cells that had been treated with preimmune serum and are the means + SD for three experiments. (B) DNA isolated from the cells was subjected to quantitative PCR analyses. Relative DNA loads of A. phagocytophilum 16s rRNA gene were normalized to DNA loads of the human β-actin gene. Results shown are the means + SD of triplicate samples and are representative of three independent experiments with similar results. Statistically significant (*, P < 0.05; **, P < 0.005; ***, P < 0.001) values relative to the bacterial load of host cells that had been incubated with preimmune antisera are presented.
Figure 7A-C. AipA residues 9-21 are critical for establishing infection in host cells. (A) Western blot analyses in which rabbit antiserum targeting AipA^i, AipA6i-84, AipAi65-i82, AipAi 83-201 , AipAi-87, or preimmune rabbit serum was used to screen whole cell lysates of uninfected (U) and A. phagocytophilum infected HL-60 cells (I). Data are representative of two experiments with similar results. (B) ELISA in which AipAg-21, AipA6 i-84, AipA|65.i82, and AipA 183-201 antibodies were used to screen wells coated with peptides corresponding to AipA residues 9-21 , 61-84, 165-182 and 183-201. Each antiserum only recognized the peptide against which it had been raised. Results shown are the mean (+ SD) of triplicate samples. Data are representative of three experiments with similar results. (C) Pretreatment of A. phagocytophilum with AipAci.21 antiserum inhibits infection of HL-60 cells. DC bacteria were pretreated with antiserum specific for AipA9-2i , AipA6i-84, AipAi65-i82, AipAi83-2oi, AipAi-87, AipA249-355, OmpA, or preimmune serum for 30 min. Next, the treated bacteria were incubated with HL-60 cells for 60 min. After removal of unbound bacteria, host cells were incubated for 24 h and subsequently examined using Msp2 (P44) antibody and confocal microscopy to assess the percentage of infected cells. Results shown are relative to preimmune serum-treated host cells and are the means + SD for six experiments. Statistically significant (*, P < 0.05; **, P < 0.005; ***, P < 0.001) values are indicated.
Figure 8A-C. OmpA amino acids 59 to 74 are critical for A. phagocytophilum to bind to sLex-capped PSGL-1 and for infection of mammalian host cells. (A) ELISA in which OmpA23-40, OmpA41 -58, and OmpA59-74 antibodies (diluted 1 : 1600) were used to screen wells coated with GST, GST-OmpA, GST-OmpA 19-74, GST-OmpA75-205, or peptides corresponding to OmpA23-40, OmpA41-58, or OmpA59-74. Results shown are the mean + SD of triplicate samples and are representative of three independent experiments with similar results. (B) Pretreatment of A. phagocytophilum with OmpA59-74 antibody inhibits infection of HL-60 cells in a dose-dependent manner. DC bacteria were incubated with 200 μg/ml of preimmune serum, 200 ^ig/ml of serum raised against GST-OmpA, or twofold serially-diluted concentrations of sera raised against OmpA23-40, OmpA41-58, or OmpA59-74 ranging from 0 to 200 μg/ml and then incubated with HL-60 cells. The infection was allowed to proceed for 24 h after which the cells were fixed and examined using immunofluorescence microscopy to quantify the percentage of infected cells. Results shown are relative to host cells that had been incubated with bacteria exposed to preimmune serum and are representative of three experiments with similar results. (C) OmpA59-74 antibody inhibits A. phagocytophilum binding to sLex-capped PSGL-1. DC bacteria were exposed to preimmune serum, antibodies against OmpA, OmpA23-40, OmpA41-58, or OmpA59-74 and then incubated with PSGL-1 CHO cells. Bacteria that were not exposed to antibodies and incubated with PSGL-1 CHO cells or CHO (-) cells were positive and negative controls, respectively, for bacterial binding. The mean numbers + SD of bound DC organisms per cell were determined using immunofluorescence microscopy. Results shown are the mean + SD of six combined experiments. Statistically significant (** P < 0.005; ***P <
0.001) values are indicated.
Figure 9A-F. Molecular docking models of A. phagocytophilum OmpA-sLe interactions. (A) Predicted tertiary structure for A. phagocytophilum OmpA. The dotted line separates the regions encompassed by residues 19 to 74 and 75 to 205. Residues K60, G61, and K64 positions are indicated. (B) Stick representation of the N-terminal PSGL-1 amino acids 61 to 77 capped with sLex derived from PDB lgls. The sLex glycan extends off of threonine 73. sLex linkages and individual sugar residues are denoted. (C) Electrostatic surface map of A.
phagocytophilum OmpA, as generated using the PyMol APBS plugin. The image is oriented as in (A). (D) The electrostatic surface map as in (C) rotated 180° around the y-axis. The dotted line is a demarcation between the regions encompassed by residues 19 to 74 and 75 to 205, which have overall cationic and anionic surface charges, respectively. (E and F) OmpAand sLex interactions predicted by the Autodock Vina algorithm. OmpA is presented as a ribbon model, sLex as a stick model, and hydrogen bonding by dotted lines. OmpA residue 64 is predicted to interact with a2, 3-1080 sialic acid of sLex (E and F). Residue G61 is predicted to interact with either a2,3-sialic acid (E) or al ,3-fucose of sLe (F). Residue 60 is predicted to interact with β1 ,3 galactose of sLex (F).
Figure 10A-B. OmpA is highly conserved among A. phagocytophilum isolates and its key binding residues exhibit variable conservation among Anaplasmataceae species. (A) Alignment of OmpA amino acid sequence from the A. phagocytophilum strain (isolated from a human patient in Massachusetts), with OmpA sequences from A. phagocytophilum strains HZ (human; New York), HGE1 (human; Minnesota), Dog (Minnesota), JM (jumping mouse;
Minnesota), MRK (horse; California), ApW&x-\ isolates CRT35 and CRT38 (both from ticks; Minnesota), and NorV2 (lamb; Norway). (B) Alignment of NCH-1 OmpA amino acids 19 to 74 with corresponding regions of OmpA homologs from the A. marginale St. Maries strain
(AM854), A. marginale Florida strain (AMF640), A. marginale subsp. centrale Israel starin (ACIS00486) E. chaffeensis Arkansas strain (ECH0462), Ehrlichia canis Jake strain (Ecaj0563), and the Ehrlichia ruminantium Welgevonden strain (Erum5620). The binding domain corresponding to NCH-1 OmpA comprises residues 59 to 74. Numbers above the alignments in (A) and (B) denote amino acid position numbers. The arrows in (A) and (B) denote d.
phagocytophilum OmpA G61and K64, which were predicted to form interactions with sLe in Figure 9 panels E and F and were shown to be critical for OmpA to bind to and mediate infection of mammaliam host cells in Figures 8 and 1 1.
Figure 11A-D. G61 and K64 are essential for recombinant OmpA to optimally bind to mammalian host cells and competitively inhibit s, phagocytophilum infection. GST1087 OmpA proteins having the CLNHL peptide inserted between OmpA amino acids 67 and 68 or having G61 and/or K64 mutated to alanine are unable to bind to competitively inhibit A.
phagocytophilum infection of mammalian host cells. HL-60 cells were incubated with DC organisms in the presence of GST alone, GST-OmpA, GST-OmpA proteins bearing insertions of CLNHL (SEQ ID NO:75) between the indicated residues (A), or GST-OmpA proteins having the indicated amino acids substituted with alanine (B and C) for 1 h. After washing to remove unbound bacteria, host cells were incubated for 24 h and subsequently examined by
immunofluorescence microscopy to determine the percentage of infected cells (A and B) or the mean number (+ SD) of morulae per cell (C). Results shown in (A), (B), and (C) are the means + SD for six to twelve combined experiments. The data presented in panel C are the normalized values of six to twelve experiments. Statistically significant (** P < 0.005; ***p < 0.001) values are indicated. (D) Flow cytometric analysis of His-OmpA and His-OmpA proteins bearing alanine substitutions binding to RF/6A cells. Data are representative of two experiments with similar results.
Figure 12A-C. Treatment with al,3/4-fucosidase reduces A. phagocytophilum binding to PSGL-1 CHO cells and binding to and infection of RF/6A endothelial cells.
PSGL-1 CHO cells (A) and RF/6A cells (B and C) were treated with al ,3/4-fucosidase (+ fucosidase) or vehicle control (- fucosidase). Fucosidase- and mock-treated cells were incubated with A. phagocytophilum DC organisms. Following the removal of unbound bacteria, the infection of RF/6A cells was allowed to proceed for 24 h prior to being assessed, while bacterial binding to PSGL-1 CHO and RF/6A cells was examined immediately. The mean + SD of bound DC bacteria per PSGL-1 CHO (A) or RF/6A cell (B) or percentage of infected RF/6A cells (C) were determined using immunofluorescence microscopy. Results shown are the means + SD for three combined experiments. Statistically significant (***P < 0.001) values are indicated.
Figure 13A-E. OmpA interacts with l,3-fucose on mammalian host cell surfaces. (A to D) RF/6A cells were treated with al ,3/4-fucosidase (A and C), a2,3/6-sialidase (B and D), or vehicle control (- fucosidase and - sialidase, respectively). Glycosidase- and mock-treated cells were incubated with al,3/6-fucose-specific lectin, AAL; the a2,3- sialic acid-specific lectin, MAL II; His-OmpA; or His-Aspl4. (A) The host cells were fixed and screened using
immmunofluorescence microscopy (A and B) or flow cytometry (C and D) to detect lectin, His- OmpA, or His-Aspl4 binding to host cells. (E) AAL and MAL II competitively inhibit His- OmpA binding to mammalian host cells. RF/6A cells were incubated with AAL and MAL II, after which His-OmpA was added. Following the removal of unbound recombinant protein, His- OmpA bound on RF/6A cell surfaces was detected by flow cytometry. Statistically significant ***P < 0.001) values are indicated. Results shown are representative of three experiments with similar results.
Figure 14A-D. OmpA interacts with 6-sulfo sLe* on RF/6A endothelial cell surfaces.
(A) Schematic representations of sLex and 6-sulfo sLex. Individual sugar and glycosidic linkages are indicated. (B and C) 6-sulfo sLex is present in high abundance relative to sLex on RF/6A cells. RF/6A cells were screened with sLe -specific antibodies, CSLEX1 and KM93; 6-sulfo sLex-specific antibody, G72; or IgM isotype control followed by detection of cell surface bound antibodies using immunofluorescence microscopy (B) and flow cytometry (C). (D) Antibody blocking of 6-sulfo sLe" inhibits His-OmpA binding to RF/6A cells. RF/6A cells were incubated with CSLEXl , KM93, G72, IgM, or vehicle (Cells only) followed by the addition of His-OmpA, and washing to remove unbound recombinant protein. Flow cytometry was used to detect bound His-OmpA. Statistically significant (*** p < 0.001) values are indicated. Results shown are representative of two experiments with similar results.
Figure 15A-D. OmpA coated beads bind to and are internalized by non-phagocytic endothelial cells. (A) Confirmation of recombinant OmpA conjugation to inert beads.
Fluorescent OmpA-coated microspheres (OmpA beads) were incubated with OmpA antibody (aOmpA) or isotype control. Unconjugated (Control) beads were included as a negative control. Bound antibody was detected using immunofluorescence microscopy. (B to D) OmpA coated beads bind to and are internalized by nonphagocytic RF/6A cells. OmpA coated beads were incubated with RF/6A cells for 1 h after which unbound beads were washed off. Cells were screened with OmpA antibody and examined using immunofluorescence (B and D) or scanning electron microscopy (C) to assess binding or were incubated further to allow for bead uptake. To assess for internalized beads, the host cells were treated with trypsin, washed, incubated overnight for the endothelial cells to re-adhere, fixed, and screened with OmpA antibody and immunofluorescence microscopy. (B) DIC, differential interference contrast microscopy. (C) Two scanning electron micrographs depicting bound or internalized OmpA coated beads. Arrows denote filopodia-like structures bound to beads. Scale bars are indicated. Results in (D) are representative of thirteen experiments with similar results. Statistically significant (** P < 0.005; ****p < 0.0001) values are indicated.
Figure 16A-I. OmpA coated bead binding to and uptake by promyelocytic HL-60 cells involve OmpA residues G61 and K64 and are dependent on sLe\ (A) Scanning electron micrographs depicting OmpA coated beads bound to and being internalized by HL-60 cells. Arrows point to filopodia-like structures adhered to beads. Scale bars are indicated. (B and C) HL-60 cells were incubated with beads coated with OmpA, OmpA proteins having the indicated amino acids substituted with alanine, or non-coated control beads. The numbers of bound and internalized beads were determined using immunofluorescence microscopy. (D to I) HL-60 cells were incubated with a2,3/6-sialidase, l,3/4-fucosidase, or vehicle only (D and E), sLe antibody CSLEX1 or IgM isotype control (F and G), or PSGL-1 N-terminus antibody KPL-1 or IgG isotype control (H and I) before being incubated with OmpA coated or non-coated control beads. The numbers of bound and internalized beads were assessed using immunofluorescence microscopy. Results in (B) through (I) are the mean + SD of triplicate samples and are representative of three independent experiments with similar results. Statistically significant (** P < 0.005; ***P < 0.001) values are indicated.
Figure 17A-D. The Aspl4 binding domain is contained within amino acids 113 to 124. (A) Pretreatment of A. phagocytophilum with Aspl41 13-124 antiserum inhibits infection of HL-60 cells in a dose-dependent manner. DC bacteria were incubated with 200 ng/ml of preimmune serum, 200 μg/ml of serum raised against full-length Asp 14, or two-fold serially- diluted concentrations of anti -Asp 1498-112 or anti-Asp 14113-124 ranging from 0 to 200 μg/ml and then incubated with HL-60 cells. The infection was allowed to proceed for 24 h prior to being assessed by immunofluorescence microscopy for the percentage of infected cells. (B) A combination of antisera targeting OmpA59-74 and Aspl 41 13-124 inhibits A. phagocytophilum infection of HL-60 cells better than serum targeting either binding domain alone. DC organisms were exposed to preimmune serum or antisera targeting OmpA59-74, Aspl41 13-124, OmpA59- 74 plus Aspl498-1 12, or anti- Aspl41 13-124 together with OmpA59-74, OmpA23-40, or OmpA43-58 antibodies. The cells were fixed and screened using immunofluorescence microscopy to determine the percentages of infected cells. (C and D) OmpA59-74 and
Aspl 4113-124 Fab fragments effectively inhibit A. phagocytophilum infection of HL-60 cells. DC bacteria were incubated with Fab fragments derived from preimmune serum, antibodies targeting OmpA23-40, OmpA41-58, OmpA59-74, Aspl498-1 12, Aspl4113-124, or OmpA59-74 Fab fragment together with Aspl4113- 124 Fab fragment. The cells were fixed and screened to determine the percentages of infected cells (C) and morulae per cell (D). Results presented in (B) to (D) are relative to host cells that had been incubated with bacteria treated with preimmune serum. Results presented in (A) and (B) are the means + SD for three experiments. Results in (C) and (D) are the mean + SD of triplicate samples and are representative of two experiments with similar results. Statistically significant (* P < 0.05; ** P < 0.005; ***P < 0.001) values are indicated.
Figure 18A-C. A combination of antisera targeting the binding domains of OmpA,
Aspl4, and AipA blocks A. phagocytophilum infection of mammalian host cells. DC organisms were incubated with preimmune serum or antibodies specific for OmpA59-74 and Asp 14113- 124; or AipA9-21 , AipA61 -84, AipA 165- 182, or AipA 183-201, either independently or in combination with OmpA59-74 and Aspl41 13-124 antibodies. Next, the bacteria were incubated with HL-60 cells. The infection was allowed to proceed for 24 h, after which the host cells were fixed and examined using immunofluorescence microscopy to determine the percentages of infected cells (A) and the number of morulae per cell (B). (C) To verify that the observed reductions in A. phagocytophilum infection were due to antisera mediated blocking of bacterial binding to HL-60 cell surfaces, the experiment was repeated except that DC organisms were incubated with antibodies targeting OmpA59-74 and/or Aspl4113-124, and/or AipA9-21 prior to being incubated with host cells, and the numbers of bound bacteria per cell was assessed. Results presented are relative to host cells that had been incubated with bacteria treated with preimmune serum and are the means + SD for six combined experiments. Statistically significant (* P < 0.05; ** P < 0.005; ***p < 0.001) values are indicated. DETAILED DESCRIPTION
The detailed characterization of the AipA protein presented herein has led to the confirmation that it is an outer membrane protein and the discovery that it is in fact an invasin protein which is instrumental in host cell invasion by A. phagocytophilum bacterium. Further, the present disclosure establishes that AipA, and suitable fragments thereof which represent domains involved in bacterial invasion, can be used as both therapeutic agents (e.g. immunogenic agents and vaccinogens to prevent or treat infection); as diagnostic agents (e.g. as targets to detect antibodies to A. phagocytophilum in biological samples); and/or to generate antibodies which are used as therapeutic agents or as diagnostic agents to detect A. phagocytophilum AipA protein in biological samples. In some aspects, AipA and/or suitable effective fragments thereof are used for these purposes in combination with one or more other invasin proteins or suitable effective invasin fragments thereof, examples of which include OmpA and Asp 14. Homologs of OmpA and Asp 14 may also be employed for these purposes.
The critical regions of OmpA and Asp 14 that mediate infection are highly conserved among family members Aph, A. marginale, and closely related Ehrlichia species, such as E. chaffeensis, E. canis, and E. ruminatium, and may be highly conserved in A. platys. In particular, Aph and A. marginale are closely related and express many gene homologs, including Asp 14 and OmpA and other surface antigens. The high degree of conservation makes these surface proteins ideal for producing a vaccine or immunogenic composition to provide protection from or therapy for multiple pathogens in humans and animals. AipA, however, has no known homologs in other Anaplasma and Ehrlichia species (all of which cause disease in humans and/or animals - cattle, horses, sheep, dogs, cats) or other non-Anaplasmataceae organisms.
Thus, AipA is unique to A. phagocytophilum and is thus not employed alone to treat or diagnose infections other than A. phagocytophilum.
Accordingly, assays and methods of using the assays to distinguish exposure to or infection with A. phagocytophilum from exposure to or infection with a non-A phagocytophilum Anaplasmataseae species are provided, e.g. to permit specialized treatment and/or to track the occurrence or frequency of A. phagocytophilum infections. A biological sample from a subject is thus screened with two or more of the agents described herein, at least one or which is AipA based or derived, and if a sample contains (is positive for the presence of) AipA proteins or antibodies to AipA proteins, it is concluded that the subject is infected with (or at least was previously exposed to) A. phagocytophilum. Previous exposure to A. phagocytophilum may result from a prior vaccination. Therefore, the methods of the invention may be used to determine if a subject has or has not been previously vaccinated. However, if the sample contains only OmpA and/or Asp 14 proteins or antibodies, but AipA proteins/antibodies are absent, a conclusion is drawn that the subject is infected or has been exposed to an Anaplasmataseae species that is not A. phagocytophilum. If the subject is currently infected with A.
phagocytophilum, then treatment specific for that organism may be undertaken, e.g.
administration of an anti- A. phagocytophilum immune response eliciting composition (e.g. compositions comprising AipA protein or fragments thereof as described herein), administration of antibodies directed specifically to AipA protein or fragments thereof, etc.
In order to facilitate the understanding of the present invention, the following definitions are provided:
Aph: Anaplasma phagocytophilum or A. phagocytophilum, an Anaplasmataseae family bacterium that is tick-born and causes anaplasmosis in humans and animals.
Apl: Anaplasma platys or A. platys, an Anaplasmataseae family member bacterium that is tick- born and causes anaplasmosis that is restricted to dogs. Anaplasmataceae: a family of closely related bacteria, including Anaplasma and Ehrlichia species. The genera Neorickettsia and Wolbachhia are also Anaplasmataceae, bacteria but do not cause anaplasmosis.
Antigen: term used historically to designate an entity that is bound by an antibody, and also to designate the entity that induces the production of the antibody. More current usage limits the meaning of antigen to that entity bound by an antibody, while the word "immunogen" is used for the entity that induces antibody production. Where an entity discussed herein is both
immunogenic and antigenic, reference to it as either an immunogen or antigen will typically be made according to its intended utility. The terms "antigen", "antigenic region" "immunogen" and "epitope" may be used interchangeably herein. As used herein, an antigen, immunogen or epitope is generally a portion of a protein (e.g. a peptide or polypeptide).
AipA: 36.9-kilodalton ^ ? ! surface protein. AipA has no known homologs in other Anaplasma and Ehrlichia species or other non-Anaplasmataceae organisms.
Aspl4: 14-kilodalton ^/z surface protein. Aspl4 homologs are expressed by Anaplasmataceae family members, including Aph, A. marginale, Ehrlichia chaffeensis, E. canis, E. ewingii, and E. ruminatium.
OmpA: Outer membrane protein A. OmpA homologs are expressed by Anaplasmataceae family members, including Aph, A. marginale, Ehrlichia chaffeensis, E. canis, E. ewingii, and E.
ruminatium.
DC and RC: Aph undergoes a biphasic developmental cycle, the kinetics of which have been tracked in promyelocytic HL-60 cells. The cycle begins with attachment and entry of an infectious dense-cored (DC) organism. Once intracellular, the DC differentiates to the noninfectious reticulate cell (RC) form and replicates by binary fission to produce a bacteria- filled organelle called a morula. Later, the RCs transition back to DCs, which initiate the next round of infection.
Epitope: a specific chemical domain on an antigen that is recognized by a B-cell receptor, and which can be bound by secreted antibody. The term as used herein is interchangeable with "antigenic determinant". An epitope may comprise a single, non-interrupted, contiguous chain of amino acids joined together by peptide bonds to form a peptide or polypeptide. Such an epitope can be described by its primary structure, i.e. the linear sequence of amino acids in the peptide chain. Epitope may also refer to conformational epitopes, which are comprised of at least some amino acids that are not part of an uninterrupted, linear sequence of amino acids, but which are brought into proximity to other residues in the epitope by secondary, tertiary and/or quaternary interactions of the protein. Residues in conformational epitopes may be located far from other residues in the epitope with respect to primary sequence, but may be spatially located near other residues in the conformational epitope due to protein folding.
Immunodominant epitope: The epitope on a molecule that induces the dominant, or most intense, immune response. The immunodominant epitope would elicit the greatest antibody titer during infection or immunization, as measured by, for example, the fraction of reactivity attributable to a certain antigen or epitope in an enzyme-linked immunosorbant assay as compared with the total responsiveness to an antigen set or entire protein.
Invasin domain: An invasin domain is a region of a pathogen's protein that binds a host cell and mediates intracellular signaling and pathogen entry into the host cell. In some cases, uptake of the pathogen results in the formation of a vacuole in which the intracellular pathogen will reside. The invasin domains of the invention are linear amino acid sequences within Asp 14, OmpA, AipA or other surface proteins that are found on the outer membrane of the bacteria Aph and other Anaplasmataceae family members, and can vary slightly from one family member to the next. However, the invasin domain in each Asp 14 homolog is critical for uptake of bacteria into host cells (known to be neutrophils and endothelial cells in the case of Anaplasmataceae).
Invasin domains may be referred to herein as "effector domains".
Inclusive: with respect to linear amino acid sequence, inclusive refers to a sequence that includes the first- and last-numbered amino acids, e.g. "amino acids 1-87 inclusive" refers to a linear amino acid sequence that includes amino acids 1 , 2, 3... etc. up to and including amino acids ...85, 86 and 87 of a sequence, e.g. a sequence specified as a SEQ ID NO: or in referenced to a SEQ ID NO:.
Linker sequences: short peptide sequences encoding functional units that may be engineered or otherwise added at the ends or within recombinant proteins, polypeptides, peptides of interest. Linker sequences may be used as "handles" for protein purification, as detectable signals of expression or binding to other proteins or macromolecules, to modulate tertiary structure, or enhance antigenicity. Examples of linker sequences include, but are not limited to, an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, and a protein purification ligand. LINKER: a program to generate linker sequences for fusion proteins. Protein Engineering 13(5): 309-312, which is a reference that describes unstructured linkers. Structured (e.g. helical) sequence linkers may also be designed using, for example, existing sequences that are known to have that secondary structure, or using basic known biochemical principles to design the linkers. Tags: Recombinant protein sequences that can be added to the N- or C-terminus of a
recombinant protein for the purpose of identification or for purifying the recombinant protein for subsequent uses. Examples of recombinant protein tags that may be useful in practicing the invention include, but are not limited to, glutathione-S-transferease (GST), poly-histidine, maltose binding protein (MBP), FLAG, V5, halo, myc, hemaglutinin (HA), S-tag, calmodulin, tag, streptavidin binding protein (SBP), Softagl™, Softag3™, Xpress tag, isopeptag, Spy Tag, biotin carboxyl carrier protein (BCCP), GFP, Nus-tag, strep-tag, thioredoxin tag, TC tag, and Ty tag. All such tags are well-known to those of ordinary skill in the art of recombinant protein production.
Protein: Generally means a linear sequence of about 100 or more amino acids covalently joined by peptide bonds.
Polypeptide: Generally means a linear sequence of about 55 to about 100 amino acids covalently joined by peptide bonds.
Peptide: Generally means a linear sequence of about 55 or fewer amino acids covalently joined by peptide bonds.
Note: The terms "peptide", "polypeptide" and "protein" may be used interchangeably herein. Chimeric or fusion peptide or polypeptide: a recombinant or synthetic peptide or polypeptide whose primary sequence comprises two or more linear amino acid sequences which do not both occur in a single molecule in nature, or at least are not linearly contiguous in a single molecule in nature. (For example, they may both be present in a single molecule which comprises e.g. amino acids 1-15, but in the fusion or chimera, "amino acids 1-5" and amino acids 10-15" are present but amino acids 6-9 are not present between the sequence represented by amino acids 1 -5 and the sequence represented by amino acids 10-15.) The two or more sequences may be, for example, a peptide (e.g. an epitope or antigenic region) and a linker sequence, or two or more peptides (which may be the same or different) which are either contiguous or separated by a linker sequence, etc. Tandem repeats: two or more copies of nucleic acid or amino acid sequences encoding the same peptide, which are arranged in a linear molecule and are either contiguous or separated by a linker sequence, etc.
Original or native or wild type sequence: The sequence of a peptide, polypeptide, protein or nucleic acid as found in nature.
Recombinant peptide, polypeptide, protein or nucleic acid: peptide, polypeptide, protein or nucleic acid that has been produced and/or manipulated using molecular biology techniques such as cloning, polymerase chain reaction (PCR), etc.
Synthetic peptide, polypeptide, protein or nucleic acid: peptide, polypeptide, protein or nucleic acid that has been produced using chemical synthesis procedures.
Type-specific: associated primarily with a single phyletic group.
Surface protein: A protein located on the outer surface membrane of a cell or bacterium.
Tables 1 , 2 and 3 provide a list of the SEQ ID NOS: for sequences described herein.
Table 1. Aph Sequence Listing with SEQ ID Numbers for Aspl4, OmpA and AipA. .
SEQ ID PROTEIN GENBANK AMINO ACID SEQUENCE ACCESSION NO NAME # AND
NAME
SEQ ID Full-length YP 504865 MIPLAPWKSISVVYMSGSDEYKEIIKQ
APH_0248 CIGSVKEVFGEGRFDDVVASIMKMQE NO:01 Asp 14
KVLASSMQQDDTGTVGQIESGEGSGA
RLSDEQVQQLMNSIREEFKDDLRAIKR
RILKLERAVYGANTPKES
SEQ ID Aspl4 aalOl- APH_0248 LRAIKRRILKLERAVYGANTPKES
NO:02 124
SEQ ID Asp 14 aal l3- APH_0248 RAVYGANTPKES
NO:03 124
SEQ ID Full length YP 504946 MLRRSSFFCLLALLSVTSCGTLLPDSN
APH_0338
NO:04 OmpA VGVGRHDLGSHRSVAFAKKVEKVYF
DIGKYDLKGPGKKVILELVEQLRQDD
SMYLWIGHADATGTEEYSLALGEKR
ANAVKQFIIGCDKSLAPRVTTQSRGK AEPEVLVYSTDAQEVEKANAQNRRA
VIVVEFAHIPRSGVADMHAPVASSITS ENSNASAEGEDMEASEFSSAIAN
SEQ ID OmpA aal9-74 APH_0338 CGTLLPDSNVGVGRHDLGSHRSVAFA NO:05 KKVEK VYFD IGKYD LKGP GKKVILEL
VEQLR
SEQ ID OmpA aa59-74 APH 0338 L GPG KVILELVEQL
NO:06
SEQ ID OmpA aa48-56 APH_0338 EKVYFDIGK
NO:07
SEQ ID OmpA APH_0338 GH AD ATGTEE YS LALG
NO:08
SEQ ID OmpA APH_0338 LVYSTDAQEVEKANAQNRRAV NO:09
SEQ ID OmpA APH 0338 PDSNVGVGRHDLGSHRSVAFAKKVE NO: 10 KVYFDIG YDLKGPGKKVILELVEQL
RQDDSMYLVVIGHADATGTEEYSLAL
GEKRANAVKQFIIGCDKSLAPRVTTQS
RGKAEPEVLVYSTDAQEVEKANAQN
RRAVIVVEFAHIPRSGVADM
SEQ ID Aspl4 aal01- APH_0248 LRAIKRRILKLE
NO: 11 112
SEQ ID Aspl4 aal9-60 APH 0248 DEYKEllKQCIGSVKEVFGEGRFDDVV NO: 12 ASIMKMQEKVLASSM
SEQ ID Full length APH_0915 MSFTMSKLSLDPTQGSHTAENIACSIF
DMVLGVKSTAKLLAGTWAGTSSTIW NO: 13 AipA
KTVTG AAS STKEAS SKS YGTLRS SLGS
SASRRMLGTCATAALCLTAPLLGAAA
AGAAITCALITICMALLFLVLYTVLHI
ASQMLRCASLLLSMVCNILHSTFTAT KSCLGG SPARTTEERVAGDLDHKGV
DSDRKHDAEKTEE HGLGSLCKSLA
INLVSLMGTALVTTPIILLAVVLLVLVP
VYLLCATVHHIYQGNYEDRNNDKGSS
RGGGTTYYPMTMSASASEESLSSIISE
GGLS TSLPSYSAATATGTGNATGEV
FSHSHSSGKSSSKPESRPESNLQNVVA
ETMSQQQRSVS
SEQ ID AipA aa9-21 APH_0915 SLDPTQGSHTAEN
NO:14
SEQ ID AipA aal-87 APH 0915 MSFTMSKLSLDPTQGSHTAENIACSIF
DM VLG VKSTAKLLAGTWAGTS STIW NO: 15
KTVTGAASSTKEASSKSYGTLRSSLGS SASRRMLG
Table 2. Asp 14 Homologs: Sequence Listing with SEQ ID Numbers
SEQ ID Anaplasma AM936 MSGEDEYKEIIRQCIGSVKEVFGEGRFD NO: 16 marginale DVVASIMKMQEKVLASSMKDGDPVG
QIAADG VGNEL YDRI ADRLEERVS QKI SEDLRIIKKRLLRLERVVLGGGSVSGD AAAHQVSGNQPSQQNSSAAAEGG
SEQ ID A. marginale AM936 LGGGSVSGDAAAHQVSGNQPSQQNSS NO:17 AAAEGG
SEQ ID A. marginale ACIS_00403 MSGEDEYKEIIRQCIGSVKEVFGEGRFD
DVVASIMKMQEKVLASSMKDGDPVG NO: 18 subspecies
QIAADGVGNELYDRIADRLEERVSQKI
Centrale SEDLRIIKKRLLRLERVVLGGGSVSGD
AAAAHQVSGNQPSQQNSSAAAEGG
SEQ ID A. marginale ACIS_00403 LGGGSVSGDAAAAHQVSGNQPSQQNS
SAAAEGG NO:19 subspecies
Centrale
SEQ ID A. marginale & AM936 & MSGEDEYKEIIRQCIGSVKEVFGEGRFD NO:20 A. marginale ACIS-00403 DWASIMKMQEKVLASSM
subspecies Centrale
SEQ ID A. marginale & AM936 & DLRIIKKRLLRLERW
NO:21 A. marginale ACIS-00403
subspecies
Centrale
SEQ ID Ehrlichia ECH_0377 MAEDDYKGVIKQYIDTVKEIVGDSKTF
DQMFESVVRIQERVMAANAQNNEDG NO:22 chaffeensis
VIDNGDQVKRIGSSTSESISNTEYKELM EELKVIKKRILRLERKILKPKEEV
SEQ ID E. chaffeensis ECH_0377 MAEDDYKGVIKQYIDTVKEIVGDSKTF
DQMFESVVRIQERVM NO:23
SEQ ID E. chaffeensis ECH_0377 ELKVIKKRILRLE
NO:24
SEQ ID E. chaffeensis ECH_0377 RKILKPKEEV
NO:25
SEQ ID E. canis Ecaj_0636 MADDEYKGVIQQYINTVKEIVSDSKTF
DQMFESVVKIQERVMEANAQNDDGSQ NO:26
VKRIGSSTSDSISDSQYKELIEELKVIKK RLLRLEHKVLKPKEGA
SEQ ID E. canis Ecaj_0636 MADDEYKGVIQQYINTVKEIVSDSKTF
DQMFESVVKIQERVM NO:27
SEQ ID E. canis Ecaj_0636 ELKVIKKRLLRLE
NO:28
SEQ ID E. canis Ecaj_0636 HKVLKPKEGA
NO:29
SEQ ID E. ruminantium Erum6320 MADEDYKGVIKQYIDTVKEIVGDSKTF
DQMFESWKIQERVMAASAQNEANGA NO:30
LVEGDSKMKRIRSADDSIAYTQSQELL EELKVLKKRIARLERHVFKSNKTEA
SEQ ID E. ruminantium Erum6320 MADEDYKGVIKQYIDTVKEIVGDSKTF
DQMFESVVKIQERVM NO:31
SEQ ID E. ruminantium Erum6320 ELKVLKKRIARLE
NO:32
SEQ ID E. ruminantium Eram6320 RHVFKSNKTEA
NO:33
Table 3. OmpA Homologs: Sequence Listing with SEQ ID Numbers
SEQ ID Anaplasma AM854 MLHRWLALCFLASFAVTGCGLFSKE V
GMDIVGVPFSAGRVEKVYFDFNKYEIKG NO:34 marginale
SGKKVLLGLVERMKADKRSTLLIIGHTD
SRGTEEYNLALGERRANAVKEFILGCDR
SLSPRISTQSRGKAEPEVLVYSSDFKEAE
KAHAQNRRVVLIVECQHSVSPKKKMAI
KWPFSFGRSAAKQDDVGSSEVSDENPV
DDSSEGIASEEAAPEEGVVSEEAAEEAPE
VAQDSSAGVVAPE
SEQ ID A. marginale AM854 LF S KEKVGMD I VG VP F S AGRVEKVYFDF
NKYEIKGS GKKVLLGLVERMKADKRST NO:35
LLII
SEQ ID A. marginale ACIS_0048 MLHRWLALCLLASLAVTGCELFNKEKV
NIDIGGVPLSAGRVEKVYFDFN IKGS NO:36 subspecies 6 KYE
GKKVLLGLVERMKADKMSTLLIVGHTD
Centrale SRGTEEYNLALGERRANAVKEFILGCDR
SLSPRISTQSRGKAEPEILVYSSDFKEAEK
AHAQNRRVVLIMECQHAASPKKARVSR
WPFSFGRSSATQQDNGGGTVAAGSPGE
DAPAEWEPEETQEAGE
SEQ ID A. marginale ACIS_0048 LFNKEKVNIDIGGVPLSAGRVEKVYFDF
NKYEIKGSGKKVLLGLVERMKADKMST NO:37 subspecies 6
LLIV
Centrale
SEQ ID A. marginale & AM854 & AGRVEKVYFDFNKYEIKGSGKKVLLGL
VERMKAD NO:38 A. marginale ACIS- subspecies 00486
Figure imgf000029_0001
NO:47 chaffeensis
SEQ ID E. canis Ecaj_0563 MKHKLVFIKFILLCLILS S CKTTDH VPL V
NTDHVFSNMKTIEKIYFDFGKATIGDSD NO:48
KAILEKVIQKAQKDTNTNIVIVGHTDTR
GTDEYNLELGEQRANAVKDFIIEHDKSL
ENRITVQSKGKSEPAVLVYSSNPEEAEH
AHAKNRRVVITLTDNGNKTSQ
SEQ ID E. canis Ecaj_0563 TTDHVPLVNTDHVFSNMKTIEKIYFDFG NO:49 KATIGDSDKAILE VIQKAQKDTNTNIVI
V
SEQ ID E. canis Ecaj_0563 GDSDKAILEKVIQ AQ DTNTNIVIV NO:50
SEQ ID E. canis Ecaj_0563 GHTDTRGTDEYNLELGE
NO:51
SEQ ID E. canis Ecaj_0563 QRANAVKDFIIEHDKSLENRITVQSKGKS
EPAV NO:52
SEQ ID E. canis Ecaj_0563 LVYS SNPEE AEHAH AKNRRVVI
NO:53
SEQ ID E. ruminantium Erum5620 MRYQLIVANLILLCLTLNGCHFNSKHVP
L VN VHNLF SNIKAIDKVYFDLDKTVIKD NO:54
SDKVLLEKLVQKAQEDPTTDllIVGHTDT
RGTDEYNLALGEQRANAVRDFIISCDKS
LEKRITVRSKGKSEPAILVYSNNPKEAED
AHAKNRRWITLVNNSTSTDNKVPTTTT
PFNEEAHNTISKDQENNTQQQAKSDNIN
NINTQQKLEQDNNNTPEVN
SEQ ID E. ruminantium Erum5620 NSKHVPLVNVHNLFSNIKAIDKVYFDLD
KTVIKDSDKVLLEKLVQKAQEDPTTDIII NO:55
V
SEQ ID E. ruminantium Erum5620 DSDKVLLEKLVQKAQEDPTTDIIIV NO:56
SEQ ID E. ruminantium Erum5620 GHTDTRGTDEYNLALGE
NO:57 SEQ ID E. ruminantium Erum5620 QRANAVRDFIISCDKSLEKRITVRS G S
EPAI NO:58
SEQ ID E. ruminantium Erum5620 LVYSN PKEAEDAHAKNRRVVI
NO:59
Aph surface proteins AipA, OmpA, and Asp 14 have been identified as mediating bacteria-host cell binding and entry. Thus, the surface proteins AipA, OmpA, and Asp 14 and fragments thereof that encompass an invasin domain, are used for diagnosing whether a patient has been suffering from Aph infection. Specifically, in one aspect, if antibodies to AipA are present in a suitable biological sample from a subject, then it can be concluded that the subject has been exposed to and/or is infected wi i Aph. In other aspects, if antibodies to AipA and one or both of OmpA and Asp 14 are identified in a suitable biological sample from a subject, then it can be concluded that the subject has been exposed to and/or is infected with ^ijo z. In some aspects, such determinations are made, e.g. by testing serum or other biological material from a subject suspected of on Aph infection by a suitable assay, such as an ELISA or immunoblot using, for example, antibodies to (e.g. specific antibodies to AipA (and optionally, to OmpA, and Asp 14). Further, antibodies can also be raised against two or more of AipA, OmpA and Asp 14, or to invasin domains from two or more of AipA, OmpA and Aspl4, e.g. combined in a chimeric protein. If the antibodies bind to or interact with proteins or peptide fragments in the sample, then it can be determined that the subject has been exposed to, infected with, and/or is currently infected with Aph. Alternatively, if one or more of the amino acid sequences described herein (e.g. a form of the sequence that is labeled with a detectable label) is exposed to a biological sample, and it is found that there are antibodies in the sample which bind to the one or more amino acid sequences, then it can be concluded that the subject from whom the sample was taken has antibodies to Aph and thus has either been exposed to Aph bacteria and/or is still infected •with Aph.
In other aspects, administration of AipA protein or suitable antigenic fragments thereof, or nucleic acids encoding the protein or fragments, is used to elicit an immunogenic response, e.g. a protective immunogenic response, in a subject. The immunogenic response may protect a naive subject from subsequent full-blown Aph infection when exposed to the bacterium (e.g. when bitten by an infected tick). Antibodies elicited by AipA administration bind to invasin proteins of bacteria that enter the bloodstream and prevent host cell invasion and establishment of a productive infection. Alternatively, administration of AipA protein or suitable antigenic fragments thereof, or nucleic acids encoding the protein or fragments, is used to provide treatment for an existing Aph infection by eliciting the production of antibodies to Aph. In some aspects, in addition to administration of AipA, one or both of OmpA and Asp 14 proteins or suitable antigenic fragments thereof, or nucleic acids encoding such proteins and fragments, may be administered. For example, all three proteins, or suitable effective fragments thereof as described herein, may be administered.
In some aspects, what is provided herein is a polypeptide or protein comprising at least one "core" sequence or segment consisting of an invasin domain sequence from one or more of AipA, Asp 14 and OmpA as described herein. In such constructs, which are generally chimeric or fusion polypeptides or proteins, the core sequence(s) is/are flanked on at least one of the amino terminus and carboxy terminus (i.e. at one or the other or both termini) by a sequence which is not found adjacent to the core sequence in nature. The sequence which is not found adjacent to the core sequence in nature may be a heterologous sequence (e.g. synthetic and/or from another species or strain) or may be a repeat or duplication of the same sequence. In some constructs, one core sequence is present alone; in other constructs, multiple core sequences of a single type (i.e. AipA, Asp 14 or OmpA) are present. In yet other constructs, one core sequence from at least two of AipA, Asp 14 and OmpA are present. In yet other constructions, multiple copies of one or more core sequence from at least two of AipA, Asp 14 and OmpA are present.
It is contemplated that virtually any protein sequence, as well as its corresponding nucleic acid sequence coding for the protein sequence that is or includes SEQ ID NO: 14 may be used as described herein. This includes the full length sequence (e.g., SEQ ID NO: 13) as well as any sequence of, for example from about 5-50 (or less than 5 or more than 50) amino acids before the beginning (amino terminus) or at the end (carboxy terminus) of the amino acid sequence of the invasin domain defined by SEQ ID NO: 14. Such sequences include one or more amino acids or amino acid sequences which are adjacent to or which flank SEQ ID NO: 14 in nature (such as those which are present in SEQ ID NO: 13). The polypeptide sequences as described herein may also be shortened on either the amino or carboxy terminus (or both) by one, two, or more amino acids to produce fragments within the context of the invention wherein the fragments produce the same or a similar protective effect. Alternatively, the polypeptide may be a chimera or fusion protein which comprises flanking amino acids sequences which are not adjacent to the native sequence in nature. For example, the adjacent sequences may be corresponding amino acids which are from different but related species (e.g. other
Anaplasmataceae); or amino acids which are from different species (e.g. from other bacteria or eukaryotes of interest, e.g. from infectious agents); or from a synthetic sequence, e.g. various tags such as histidine or glutathione S-transferase (GST) tags, linkers, spacers, targeting sequences, etc. as described elsewhere herein).
Further, a single recombinant polypeptide may comprise one copy, or more than one copy (a plurality, two or more) of each of these different sequences, all of which include SEQ ID NO: 14. A single polypeptide may contain two or more copies of a single sequence, a single copy of one sequence and two or more copies of one or more different sequences, or two or more copies of at least two different sequences. In exemplary embodiments, a polypeptide of the invention includes one or more copies of SEQ ID NO: 14 and optionally one or more copies of at least one of SEQ ID NO:03 and SEQ ID NO:06 on a single polypeptide or multiple polypeptides. Another aspect of the disclosure provides a mixture of at least two of any of the peptides and/or polypeptides described herein.
In some embodiments, the composition consists of or comprises three polypeptides with amino acid sequences encoding an AipA, OmpA, or Asp 14 invasin domain. The Asp 14 invasin domain lies within aal 13-124 (SEQ ID NO:03). In other embodiments, polypeptide fragments such as Aspl4 aalOl-124 (SEQ ID NO:02) or the full length protein (SEQ ID NO:01) are used. In another embodiment, the composition of the invention comprises the invasin domain of OmpA, which lies within aa59-74 (SEQ ID NO:04). In other embodiments, a larger fragment of OmpA encompassing aal 9-74 (SEQ ID NO:05), or the full length OmpA protein (SEQ ID NO:06) is used.
There is currently no means for preventing transmission of the bacteria causing anaplasmosis or HGA. While antibiotic treatments exist, these treatments are not advised for some groups of patients. In one embodiment, the invention is a vaccine for prevention or treatment of anaplasmosis and HGA. One embodiment of the invention is a pharmaceutically acceptable composition comprising one or a plurality of any one of or a mixture of at least two amino acid sequences which are or include the amino acid sequences which are identified as SEQ ID NO:01 , 02, 03, 04, 05, 06, 13, 14, or 15. Administration of the composition of the invention stimulates an immune response in a subject and production of antibodies against AipA, Asp 14, OmpA, or all three. Because AipA, OmpA, and Asp 14 are on the outer surface of Aph bacteria, antibodies produced by the subject will block binding of bacteria to host cells and interfere with uptake into vacuoles. Bacteria unable to enter host cells are detected by the host immune system and cleared from the body. Blockade can occur at the point of entry into neutrophils or endothelial cells or transfer between these two host cell types. Interruption of the zoonotic life cycle provides a further benefit to public health and well-being by breaking the chain of disease transmission to others.
Aside from commercial assays to detect Apl in dogs, there is no specific assay to rapidly confirm Anaplasmataceae infection, or accurately diagnose HGA or anaplasmosis. In one embodiment, the invention provides a method to detect the presence of Aph AipA, OmpA, and/or Asp 14 in assays of biological samples obtained from subjects to bind to antibodies produced by an Anaplasmataceae-infected individual, either of which would be diagnostic for HGA or anaplasmosis. The preferred composition for diagnostic testing may comprise full length AipA (SEQ ID NO: 13), Asp 14 (SEQ ID NO: 3), and/or OmpA (SEQ ID NO:06). However, compositions comprising fragments of AipA such as SEQ ID NO: 14 or a mixture of at least two of SEQ ID NO: 13, 14, and 15 are also contemplated. Likewise, a composition comprising fragments of OmpA, such as SEQ ID NO:04 and/or 05, and any mixtures of at least two of SEQ ID NO:04, 05, and 06 are also contemplated. Fragments of Aspl4, such as SEQ ID NO:01 and/or 02, are also contemplated, as are any mixtures of at least two of SEQ ID NO:01, 02, and 03. The assay used to detect antibodies may be any type of immunoassay, such as an immunoblot or an enzyme-linked immunosorbent assay. The test sample may be any type of body fluid, such as blood, plasma, serum, urine, saliva, or other body fluid. Tissues or cells may also be used, such as tissue sections or cell preparations adhered to slides or coverslips for immunohistochemical staining. In an exemplary embodiment, the assay used is an ELISA with each protein type to independently detect antibodies to AipA, Asp 14, and OmpA, however, a combination to detect AipA, Asp 14, and OmpA antibodies in one ELISA is also contemplated.
In addition to sequences for OmpA, Asp 14, and AipA shown in Table 1, and homologs shown in Tables 2-3, other surface proteins that Aph preferentially expresses in human versus tick cells may be used. Table 4 shows examples of proteins that can be included in the "cocktail" of peptides, polypeptides or protein sequences of the composition of the invention. Examples of these include APHJ325 (Msp2), APHJ235, APHJ378, APHJ412, APH_0346, APH_0838, APH_0839, APH_0874, and APH_0906 because all are upregulated 3- to 60-fold during RC-DC transition, DC exit, and/or reinfection and our surface proteomic study indicates that they are surface proteins. The file names for each of the aforementioned proteins are from the A.
phagocytophilum HZ annotated genome. A similar expression profile is exhibited by APH_1235, which is another late stage gene that is upregulated 70-fold (Troese et al., 201 1). Studies by Mastronunzio and colleagues suggest that APH_1235 is an A. phagocytophilum surface protein. P44 is a 44 kilodalton surface protein and is the bacterium's major surface protein. Synonyms of P44 are Msp2 (major surface protein 2) and Msp2 (P44). All Anaplasma species encode P44 proteins and there are huge repertoires of P44 genes in these bacterial species' chromosomes. For instance, the annotated Aph strain HZ genome encodes 113 P44 proteins. These exist as complete genes or pseudogenes (incomplete genes). There is one expression site for p44 genes. Basically, different p44 genes get shuffled into the expression site by a process known as gene conversion with the end result being that Aph (and other Anaplasma species) can vary the P44 protein on their cell surfaces, a process called antigenic variation. This enables them to perpetually evade the humoral immune response.
Table 4. Anaplamatacaea Surface Proteins Sequence Listing and SEQ ID Numbers
SEQ ID NO:60 Full-length APHJ 378 Genbank Accession No: YP_505877
SEQ ID NO:61 Full-length APHJ 412 Genbank Accession No: YP 505903
SEQ ID NO:62 Full-length APH_0346 Genbank Accession No: YP_504953
SEQ ID NO:63 Full-length APH_0838 Genbank Accession No: YP_505415
SEQ ID NO:64 Full-length APH_0839 Genbank Accession No: YP_505416
SEQ ID NO:65 Full-length APH_0874 Genbank Accession No: YP_505450
SEQ ID NO:66 Full-length APH_0906 Genbank Accession No: YP_505479
SEQ ID NO:67 Full-length APH_1325 Genbank Accession No: YP_505833
(Msp2)
SEQ ID NO:68 Full-length APH l 235 Genbank Accession No: YP_505764 In addition to polypeptides sequences from Aph surface proteins, other sequences may be included in the polypeptides of the invention. Such sequences include but are not limited to antigenic peptide sequences such as linker sequences which in and of themselves are antigenic. Examples of recombinant protein tags that may be useful in practicing the invention include but are not limited to glutathione-S-transferease (GST), poly-histidine, maltose binding protein (MBP), FLAG, V5, halo, myc, hemaglutinin (HA), S-tag, calmodulin, tag, streptavidin binding protein (SBP), Softagl™, Softag3™, Xpress tag, isopeptag, Spy Tag, biotin carboxyl carrier protein (BCCP), GFP, Nus-tag, strep-tag, thioredoxin tag, TC tag, and Ty tag. Examples of linker sequences include but are not limited to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, and a protein purification ligand. It should also be recognized that a multitude of other such sequences are known to those of skill in the art, and inclusion of other antigenic, linker, or tag sequences is contemplated.
Those of skill in the art will recognize that, while in some embodiments of the invention, the amino acid sequences that are chosen for inclusion in the polypeptides of the invention correspond exactly to the primary amino acid sequence of the original or native sequences of an AipA, Aspl 4, or OmpA protein, this need not always be the case. The amino acid sequence of an epitope that is included in the polypeptides of the invention may be altered somewhat and still be suitable for use in the present invention. For example, certain conservative amino acid substitutions may be made without having a deleterious effect on the ability of the polypeptides to elicit an immune response. Those of skill in the art will recognize the nature of such conservative substitutions, for example, substitution of a positively charged amino acid for another positively charged amino acid (e.g. for R or vice versa); substitution of a negatively charged amino acid for another negatively charged amino acid (e.g. D for E or vice versa);
substitution of a hydrophobic amino acid for another hydrophobic amino acid (e.g. substitution of A, V, L, I, W, etc. for one another); etc. All such substitutions or alterations of the sequences of the polypeptides that are disclosed herein are intended to be encompassed by the present invention, so long as the resulting polypeptides still function to elicit a suitable immune response. In addition, the amino acid sequences that are included in the polypeptides or any chimeric proteins of the invention need not encompass a full length native polypeptide. Those of skill in the art will recognize that truncated versions of amino acid sequences that are known to be or to contain antigenic polypeptides may, for a variety of reasons, be preferable for use in the practice of the invention, so long as the criteria set forth for an epitope is fulfilled by the sequence. Amino acid sequences that are so substituted or otherwise altered may be referred to herein as "based on" or "derived from" the original wild type or native sequence. In general, the AipA, Asp 14, or OmpA proteins or polypeptide fragments from which the linear epitopes are "derived" or on which the linear epitopes are "based" are the AipA, Aspl4, or OmpA proteins or peptide fragments as they occur in nature. These natural AipA, Asp 14, or OmpA proteins may alternatively be referred to as native or wild type proteins.
Such changes to the primary sequence may be introduced for any of a variety of reasons, for example, to eliminate or introduce a protease cleavage site, to increase or decrease solubility, to promote or discourage intra- or inter-molecular interactions such as folding, ionic interactions, salt bridges, etc, which might otherwise interfere with the presentation and accessibility of the individual epitopes along the length of a peptide or polypeptide. All such changes are intended to be encompassed by the present invention, so long as the resulting amino acid sequence functions to elicit a protective antibody response in a host to whom it is administered. In general, such substituted sequences will be at least about 50% identical to the corresponding sequence in the native protein, preferably about 60 to 70, or even 70 to 80, or 80 to 90% identical to the wild type sequence, and preferably at least about 95, 96, 97, 98, 99, or even 100% identical to a native AipA, Asp 14, or OmpA sequence or peptide fragment. The reference native AipA, Asp 14, or OmpA sequence or peptide fragment may be from any suitable type of Anaplasmataceae, e.g. from any Anaplasmataceae which is known to infect mammals.
In some embodiments of the invention, individual linear epitopes in a chimeric vaccinogen are separated from one another by intervening sequences that are more or less neutral in character, i.e. they do not in and of themselves elicit an immune response to Anaplasmataceae. Such sequences may or may not be present between the epitopes of a chimera. If present, they may, for example, serve to separate the epitopes and contribute to the steric isolation of the epitopes from each other. Alternatively, such sequences may be simply artifacts of recombinant processing procedures, e.g. cloning procedures. Such sequences are typically known as linker or spacer peptides, many examples of which are known to those of skill in the art. See, for example, Crasto, C. J. and J. A. Feng. 2000.
In addition, other elements may be present in chimeric proteins, for example leader sequences or sequences that "tag" the protein to facilitate purification or detection of the protein, examples of which include but are not limited to tags that facilitate detection or purification (e.g. S-tag, Flag-tag, Histidine tags,), other antigenic amino acid sequences such as known T-cell epitope containing sequences and protein stabilizing motifs, etc. In addition, the chimeric proteins may be chemically modified, e.g. by amidation, sulfonylation, lipidation, or other techniques that are known to those of skill in the art.
The invention further provides nucleic acid sequences that encode the proteins, protein fragments, polypeptides, peptides and/or chimeric proteins described herein. Such nucleic acids include DNA, cDNA, RNA (e.g. mRNA), and/or hybrids thereof, and the like, both single and double stranded, and complements thereof. Further, the invention comprehends vectors which contain or house one or more of such coding sequences, or sequences complementary to the coding sequences. Examples of suitable vectors include but are not limited to plasmids, cosmids, viral based vectors, expression vectors, etc. In some embodiments, the vector is a plasmid expression vector. In other aspects, the vector is a vector suitable for administration to a mammal such as a human, e.g. an attenuated viral vector such as an adenoviral, retroviral, herpes simplex, or other viral vector which is replication competent. In other aspects, the vector is a host cell harboring the nucleic acid. The encoding sequences are typically configured within the vectors so as to be translatable, e.g. by being operationally connected to a functional promoter sequence or region. The vectors may or may not be capable of replication, but are capable of being translated to produce the encoded polypeptide(s).
Nucleic acids encoding the sequences of interest may also be present in or associated with, for example, lipoplexes, polymersomes, polyplexes, dendrimers, organic and inorganic nanoparticles, etc.
The invention also provides host cells containing the nucleic acids of the invention. The host cell can be any cell that can grow in culture, be transformed or transfected with
heterologous nucleotide sequences and can express those sequences. These include bacteria, such as E. coli, Bacillus, yeast and other fungi, plant cells, insect cells, and mammalian cells. In addition, expression of these sequences in higher eukaryotic host cells may be transient or stable.
The chimeric proteins of the invention may be produced by any suitable method, many of which are known to those of skill in the art. For example, they may be chemically synthesized, or produced using recombinant DNA technology (e.g. in bacterial cells, in cell culture (mammalian, yeast or insect cells), in plants or plant cells, or by cell-free prokaryotic or eukaryotic-based expression systems, by other in vitro systems, etc.). In some embodiments, the polypeptides are produced using chemical synthesis methods.
The present invention also provides compositions for use in eliciting an immune response. The compositions comprise one or more peptides, polypeptides, or proteins as described herein, or nucleic acids encoding them (e.g. as the nucleic acid per se, or in a vector such as a host cell comprising the nucleic acid). The compositions may be utilized as
immunogenic composition and/or vaccines to prevent or treat anaplasmosis, particularly when manifested in humans as HGA. By eliciting an immune response, it is meant that administration of the antigen causes the synthesis of specific antibodies (at a titer as described above) and/or cellular proliferation, as measured, e.g. by 3H thymidine incorporation, or by other known techniques. By "vaccine" we mean a linear polypeptide, a mixture of linear polypeptides or a chimeric or fusion polypeptide that elicits an immune response, which results in protection of an organism against challenge with an Anaplasmataceae species bacterium. The protective response either wholly or partially prevents or arrests the development of symptoms related to
anaplasmosis or HGA infection (i.e. the symptoms of anaplasmosis), in comparison to a non- vaccinated (e.g. adjunct alone) control organisms, in which disease progression is not prevented. The compositions include one or more isolated and substantially purified polypeptides or chimeric peptides as described herein, and a pharmacologically suitable carrier. The polypeptides or chimeric peptides in the composition may be the same or different, i.e. the composition may be a "cocktail" of different polypeptides or chimeric peptides, or a composition containing only a single type of polypeptide or chimeric peptide. The preparation of such compositions for use as vaccines is well known to those of skill in the art. Typically, such compositions are prepared either as liquid solutions or suspensions, however solid forms such as tablets, pills, powders and the like are also contemplated. The liquid may be an aqueous liquid. Solid forms suitable for solution in, or suspension in, liquids prior to administration may also be prepared. The preparation may also be emulsified. The active ingredients may be mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredients. Suitable excipients are, for example, water, saline, dextrose, glycerol, ethanol and the like, or combinations thereof. In addition, the composition may contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, and the like. The vaccine preparations of the present invention may further comprise an adjuvant, suitable examples of which include but are not limited to Seppic, Quil A, Alhydrogel, etc. If it is desired to administer an oral form of the composition, various thickeners, flavorings, diluents, emulsifiers, dispersing aids or binders and the like may be added. The composition of the present invention may contain any such additional ingredients so as to provide the composition in a form suitable for administration. The final amount of polypeptides or chimeric peptides in the formulations may vary. However, in general, the amount in the formulations will be from about 0.01-99%, weight/volume.
The methods involve administering a composition comprising proteins, protein fragments (peptides), recombinant polypeptides and/or chimeric peptides described herein, or by
administering nucleic acids encoding these proteins, fragments and recombinant polypeptides and chimeric peptides, in a pharmacologically acceptable carrier to a mammal. The nucleic acids may be housed in a vector (e.g. a host cell) as described herein. The mammal may be a human, but this need not always be the case. Because anaplasmosis is a zoonotic disease that causes anaplasmosis in all known mammalian hosts, veterinary applications of this technology are also contemplated. The vaccine preparations of the present invention may be administered by any of the many suitable means which are well known to those of skill in the art, including but not limited to by injection, inhalation, orally, intranasally, by ingestion of a food product containing the polypeptides or chimeric peptides, etc. In some embodiments, the mode of administration is subcutaneous or intramuscular. In addition, the compositions may be administered in conjunction with other treatment modalities such as substances that boost the immune system, various anti- bacterial chemotherapeutic agents, antibiotics, and the like.
The present invention provides methods to elicit an immune response to
Anaplasmataceae and/or to vaccinate against Anaplasmataceae infection in mammals. In one embodiment, the mammal is a human. However, those of skill in the art will recognize that other mammals exist for which such vaccinations would also be desirable, e.g. the preparations may also be used for veterinary purposes. Examples include but are not limited to companion "pets" such as dogs, cats, etc.; food source, work and recreational animals such as cattle, horses, oxen, sheep, pigs, goats, and the like; or wild animals that serve as a reservoir of Anaplasmataceae, particularly wild animals adapted to living in close proximity to urban areas (e.g. mice, deer, rats, raccoons, opossum, coyotes, etc). The compositions of the invention may be used as bait vaccines, such as bait containing any of the vaccine preps described herein (e.g. mouse bait, deer bait, etc.) Also, animals in zoos and reserves may benefit from protection against Anaplasmataceae infection.
The invention also provides a diagnostic and a method for using the diagnostic to identify individuals who have antibodies to the epitopes contained within the polypeptides or chimeric proteins of the invention. A biological sample from an individual (e.g. a human, a deer, or other mammals susceptible to infection by Anaplasmataceae) suspected of having been exposed to Anaplasmataceae, or at risk for being exposed to Anaplasmataceae, is contacted with the peptides, polypeptides, or chimeric proteins of the invention. Using known methodology, the presence or absence of a binding reaction between the polypeptides or chimeric proteins and antibodies in the biological sample is detected. A positive result (i.e. binding occurs, thus antibodies are present) indicates that the individual has been exposed to and has previously mounted an immune response to, and/or is infected with Anaplasmataceae. Further, the diagnostic aspects of the invention are not confined to clinical use or home use, but may also be valuable for use in the laboratory as a research tool, e.g. to identify Anaplasmataceae bacteria isolated from ticks, to investigate the geographical distribution of Anaplasmataceae species and strains, etc.
The present invention also encompasses antibodies to the epitopes and/or to the polypeptides or chimeric proteins disclosed herein. Such antibodies may be polyclonal, monoclonal, or chimeric, and may be generated in any manner known to those of skill in the art. In a preferred embodiment of the invention, the antibodies are bactericidal, i.e. exposure of
Anaplasmataceae bacteria to the antibodies causes death of the bacteria. Such antibodies may be used in a variety of ways, e.g. as detection reagents to diagnose prior exposure to
Anaplasmataceae, as a reagent in a kit for the investigation of Anaplasmataceae, to treat Anaplasmataceae infections, etc.
Alternatively, appropriate antigen fragments or antigenic sequences or epitopes may be identified by their ability, when included in polypeptides or chimeric proteins, to elicit suitable antibody production to the epitope in a host to which the polypeptides or chimeric proteins are administered. Those of skill in the art will recognize that definitions of antibody titer may vary. Herein, "titer" is taken to be the inverse dilution of antiserum that will bind one half of the available binding sites on an ELISA well coated with 100 ng of test protein. In general, suitable antibody production is characterized by an antibody titer in the range of from about 100 to about 100,000, and preferably in the range of from about 10,000 to about 10,000,000. Alternatively, and particularly in diagnostic assays, the "titer" should be about three times the background level of binding. For example, to be considered "positive", reactivity in a test should be at least three times greater than reactivity detected in serum from uninfected individuals. Preferably, the antibody response is protective, i.e. prevents or lessens the development of symptoms of disease in a vaccinated host that is later exposed to Anaplasmataceae, compared to an unvaccinated host.
Before exemplary embodiments of the present invention are described in greater detail, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.
Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.
All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed.
It is noted that, as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as "solely," "only" and the like in connection with the recitation of claim elements, or use of a "negative" limitation.
As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present invention. Any recited method can be carried out in the order of events recited or in any other order which is logically possible.
The invention is further described by the following non-limiting examples which further illustrate the invention, and are not intended, nor should they be interpreted to, limit the scope of the invention.
EXAMPLE 1. Anaplasma phagocytophilum surface protein AipA mediates invasion of mammalian host cells
SUMMARY
Anaplasma phagocytophilum, which causes granulocytic anaplasmosis in humans and animals, is a tick-transmitted obligate intracellular bacterium that mediates its own uptake into neutrophils and non-phagocytic cells. Invasins of obligate intracellular pathogens are attractive targets for protecting against or curing infection because blocking the internalization step prevents survival of these organisms. The complement of A. phagocytophilum invasins is incompletely defined. Here, the significance of a novel A. phagocytophilum invasin protein, AipA is reported. A. phagocytophilum induced aipA expression during transmission feeding of infected ticks on mice. The bacterium upregulated aipA transcription when it transitioned from its non-infectious reticulate cell morphotype to its infectious dense-cored morphotype during infection of HL-60 cells. AipA localized to the bacterial surface and was expressed during in vivo infection. Of the AipA regions predicted to be surface-exposed, only residues 1 to 87 (AipAi-87) were found to be essential for host cell invasion. Recombinant AipAi-87 protein bound to and competitively inhibited A. phagocytophilum infection of mammalian cells. Antiserum specific for AipAi-87, but not other AipA regions, antagonized infection. Additional blocking experiments using peptide-specific antisera narrowed down the AipA invasion domain to residues 9 to 21. An antisera combination targeting AipAi.87 together with two other A phagocytophilum invasins, OmpA and Asp 14, nearly abolished infection of host cells. This study identifies AipA as an A. phagocytophilum surface protein that is critical for infection, demarcates its invasion domain, and establishes a rationale for targeting multiple invasins to protect against granulocytic anaplasmosis.
INTRODUCTION
Anaplasma phagocytophilum is an obligate intracellular bacterium in the order
Rickettsiales and family Anaplasmataceae that infects neutrophils to cause granulocytic anaplasmosis in humans and animals. Though primarily an Ixodes spp. tick-borne illness (Truchan et al, 2013), human granulocytic anaplasmosis (HGA) can also be transmitted perinatally, nosocomially, and through blood transfusion (Annen et al, 2012; Carlyon, 2012;
Jereb et al , 2012; Alhumaidan et al, 2013). The disease presents as a non-specific febrile illness that can be accompanied by leukopenia, thrombocytopenia, elevated levels of serum
transaminases, and increased susceptibility to potentially fatal secondary infections (Truchan et al, 2013). HGA is an emerging infection in the United States, Europe, and Asia (Truchan et al, 2013). The number of reported HGA cases in the United States increased over six-fold from
2003 to 2012, the latest period for which disease reporting statistics are available (Hopkins et al, 2005; Centers for Disease Control and Prevention, 2013).
A. phagocytophilum undergoes a biphasic developmental cycle that begins when an infectious dense-cored (DC) organism binds to and enters its host cell, where it resides within a host cell-derived vacuole. Between 4 and 8 h, the DC develops into the non-infectious reticulate cell (RC) morphotype that subsequently divides by binary fission to yield a bacteria-filled vacuolar inclusion. From 8 to 20 h, the intravacuolar population consists exclusively of replicating RCs. Most RCs transition back into DCs between 28 and 32 h. DCs then exit host cells between 28 to 36 h and initiate the next round of infection (Troese et al, 2009). A.
phagocytophilum OMPs that are upregulated during RC-to-DC transition, bacterial exit, and reinfection are attractive targets to evaluate for both their roles in infection and their prospect as protective antigens.
Given the potential severity of HGA, the limited choices of antibiotics for treating the disease, and the lack of a vaccine, a thorough understanding of A. phagocytophilum cellular invasion is critical. OmpA (APH0338) and Aspl4 (14-kDa A phagocytophilum surface protein; APH0248) were recently identified as being important for A. phagocytophilum entry into mammalian cells (Ojogun et at, 2012; Kahlon et at, 2013). OmpA binds to a2,3-sialic acid of the sialyl Lewis x (sLex) tetrasaccharide that caps P-selectin glycoprotein ligand-1 (PSGL-1) on myeloid cell surfaces. OmpA also recognizes a2, 3 -sialic acid residues that decorate
glycoproteins on endothelial cells (Ojogun et al, 2012). The Aspl4 receptor is unknown.
Evidence implicates involvement of one or more A. phagocytophilum invasins in addition to OmpA and Aspl4 in mediating infection (Kahlon et at, 2013). Identifying these invasins and evaluating if targeting them alone or in concert with OmpA and Asp 14 can block bacterial entry will lead to the development of effective prophylaxes against HGA.
A whole genome transcriptional profiling study revealed A. phagocytophilum genes that are upregulated during infection of mammalian versus tick cells (Nelson et at, 2008). Several of these encode putative OMPs, one of which is APH0915. In this study, it is shown that APH0 15, hereafter referred to as AipA (A. phagocytophilum invasion protein A), is important for bacterial entry into mammalian cells and identify its invasion domain. It is further demonstrated that a combination of antisera targeting AipA, OmpA, and Asp 14 synergistically blocks infection. These findings not only advance understanding of how A. phagocytophilum employs multiple invasins to promote infection, but also results in the development of a multi-target vaccine that protects against granulocytic anaplasmosis.
MATERIALS AND METHODS
Cultivation of uninfected and A. phagocytophilum-/n/ectei/ host cell lines
Human promyelocyte HL-60 cells (CCL-240; American Type Culture Collections
(ATCC), Manassas, VA), RF/6A (rhesus monkey choroidal endothelial cells, ATCC CRL-1780), ISE6 cells and phagocytophilum (NCH-1 strain) infected HL-60, RF/6A, or ISE6 cells were cultured as previously described (Huang et at, 2012). PSGL-1 CHO cells and untransfected CHO cells were maintained as previously described (Troese et at, 2009).
In silico analyses of AipA
The AipA sequence was assessed for transmembrane domains using the TMpred and TMHMM algorithms (Hofmann et at, 1993; Krogh et at, 2001), each of which yielded highly similar predictions. Results obtained using TMpred are presented in Figure 1. Protean, which is part of the Lasergene® software package (version 8.02; DNASTAR®, Madison, WI), was used to assess AipA for regions of hydrophobicity and probability of being surface-exposed using the Kyte-Doolittle (Kyte et at, 1982) and Emini (Emini et at, 1985) algorithms, respectively. Recombinant protein and antiserum production
A PCR amplicon of aipA (aph0915) nucleotides 1 to 261, encoding AipA amino acids 1 to 87, was generated using primers 5 ' -C ACCTTGAGTTTT AC AATGTCGAAGTTATCGC-3 ' (SEQ ID NO:69; the first four nucleotides correspond to a Gateway entry vector-compatible sequence) and 5 ' -CTATCCTAGCATCCTTCTAGAAGCGGAAG-3 ' (SEQ ID NO:70; the first three nucleotides denote an added stop codon). A PCR product corresponding to aipA
nucleotides 745 to 1068, encoding AipA amino acids 249 to 355, was generated using primers 5 ' -CACCATCTATCAAGGAAATTACGAAGATCGC AAC-3 ' (SEQ ID NO:71) and 5'- GAGCAGCATGCTTTA-3 ' (SEQ ID NO:72). The amplicons were cloned into the pDest-15 vector (Life Technologies, Carlsbad, CA) downstream of and in frame with the gene encoding GST as described previously (Ojogun et al., 2012). Expression and purification GST-tagged AipA residues 1 to 87 (GST-AipAi.g7), 249 to 355 (GST-AipA249-355), and GST alone were performed as previously described (Troese et al, 2011). GST-tagged full-length AipA and AipA amino acids 165 to 204 remained insoluble over a wide range of conditions and thus could not be purified. Generation of murine polyclonal antisera against each GST fusion protein was performed as described previously (Troese et al, 2011). Rabbit polyclonal antisera were raised against synthetic keyhole limpet hemocyanin ( LH)-conjugated peptides corresponding to AipA amino acid residues 9 to 21, 61 to 84, 165 to 182, and 185 to 201 (New England Peptide, Gardner, MA). Specificity of each AipA peptide antiserum for its target peptides was determined by the enzyme-linked immunosorbent assay using the TMB substrate kit (Thermo Scientific, Waltham, MA) according to the manufacturer's instructions.
Differential aipA expression studies
HL-60 cells were synchronously infected with A. phagocytophilum DC organisms (Troese et al, 2009). The infection time course was allowed to proceed for 36 h, a time period that enabled the bacteria to complete their biphasic developmental cycle and initiate a second round of infection (Troese et al, 2009). RNA isolated from aliquots taken every 4 hours was subjected to reverse transcriptase-quantitative PCR (RT-qPCR) as described previously (Troese et al, 2011) using AipA specific primers 5 ' -CCTCAACTAAAGAAGCGTC ATC AAA-3 ' (SEQ ID NO:73) and 5 '-GTACGGTGTACAAAACGAGGAACA-3 ' (SEQ ID NO:74), which targeted nucleotides 179 to 388. Relative aipA transcript levels were normalized to the transcript levels of the A. phagocytophilum 16s rRNA gene (aphlOOO) using the 2'AACT method (Livak et al, 2001 ; Kahlon et al, 2013). To determine if aipA was transcriptionally upregulated in the DC versus RC morphotype, normalized aipA transcript levels were calculated as fold changes in expression relative to expression at 16 h, a time point at which the entire A. phagocytophilum population existed in the RC form (Troese et al, 2009; Mastronunzio et al, 2012). aipA expression during blood meal acquisition by A. phagocytophilum infected nymphs from mice was monitored as described (Mastronunzio et al, 2012) using AipA primers targeting nucleotides 179 to 388. As a control, expression of the A. phagocytophilum groEL gene
(aph0240) during tick transmission feeding was monitored using gene-specific primers (Kahlon et al, 2013).
Western blotting and confocal microscopy
Antisera generated in this study and prior studies targeted AipA, APH0032 (Huang et al, 2010b), Asp55 (Ge et al, 2007), and Msp2 (P44) (Troese et al, 2011). Sera from an HGA patient and a dog that had been naturally infected with A. phagocytophilum were previously described (Ojogun et al, 2012). Western blot analyses (Troese et al, 2011) were performed on uninfected or A. phagocytophilum infected host cells or A phagocytophilum DC organisms that had been subjected to surface trypsinolysis as described previously (Kahlon et al, 2013). A. phagocytophilum infected host cells were analyzed by spinning disk confocal microscopy as described (Huang et al, 2010a; Huang et al, 2012).
AipA antiserum inhibition of A. phagocytophilum infection
Inhibition of host cell infection by preincubating DC organisms with heat- inactivated polyclonal antiserum targeting GST-AipAi_87, GST-AipA249-3 5, GST-OmpA (Ojogun, 2012), GST-Aspl4 (Kahlon et al, 2013), AipA9.2i, AipA6i-84, AipAi65-i82, or AipAi8 -2oi (2 mg mL"1) was assessed as previously described (Ojogun et al, 2012). Serum against GST alone and preimmune serum served as negative controls. In instances where DC bacteria were incubated with combinations of antisera targeting AipA, Asp 14, and/or OmpA, each respective antiserum was at a concentration of 2 mg mL"1, and control antiserum was matched accordingly. Following antibody treatment, bacterial adhesion to and infection of HL-60 cells were monitored using spinning disk confocal microscopy (Ojogun et al, 2012).
Binding of GST-AipA to mammalian host cells and competitive inhibition of A. phagocytophilum infection
Mammalian host cells cells were incubated with 4 μΜ GST, GST-AipAi-87, or GST- AipA249-355 for 1 h at 37°C. Spinning disk confocal microscopy and flow cytometry were used to assess the binding of recombinant proteins to host cells and competitive inhibition of A.
phagocytophilum infection as previously described (Ojogun et al, 2012; Kahlon et al, 2013). Assessment of the relevance of AipA to A. phagocytophilum adherence to PSGL-1 CHO cells To determine if AipA was important for A. phagocytophilum recognition of sLex-capped
PSGL-1, DC organisms were incubated with antiserum targeting AipA]-87, AipA249-355, OmpA (Ojogun et al, 2012), or preimmune control serum as described above. Next, the treated bacteria were incubated with PSGL-1 CHO cells or untransfected CHO cells for 1 h, followed by two rounds of washing with PBS to remove unbound bacteria, and enumeration of bound organisms using spinning disk confocal microscopy as described (Troese et al, 2009). As positive controls for inhibition of bacterial adherence to sLex-capped PSGL-1, PSGL-1 CHO cells were incubated with the PSGL-1 N-terminus-specific antibody, KPL-1 (BD Biosciences, San Jose, CA), or the sLex-specific antibody, CSLEX1 (BD Biosciences) for 30 min prior to the addition of bacteria. Mouse IgG and mouse IgM served as isotype controls for KPL-1 and CSLEX1 , respectively. Statistical analyses
One-way analysis of variance (ANOVA) was performed using the Prism 5.0 software package (Graphpad; San Diego, CA) to assess statistical significance as described (Ojogun et al, 2012). Statistical significance was set at P < 0.05.
RESULTS
A. phagocytophilum differentially expresses AipA during the infectious stage of its biphasic developmental cycle, the transmission bloodmeal of infected ticks, and infection of mammalian hosts
AipA is a 355-amino acid (36.9 kDa) protein and a putative OMP (Nelson et al, 2008). In silico analysis of the AipA amino acid sequence predicted that residues 107 to 127, 136 to 155, and 220 to 243 form transmembrane domains that position residues 1 to 106 and 156 to 219 on the bacterial surface (Figure 1 A). Protein BLAST searches revealed that AipA does not display high sequence identity to sequenced proteins of other organisms, including
Anaplasmataceae and Rickettsiales members. Because A. phagocytophilum proteins encoded by genes that are upregulated late during the biphasic developmental cycle are important for infection (Huang et al, 2010b; Troese et al, 2011 ; Mastronunzio et al, 2012), AipA expression throughout the infection cycle in human promyelocytic HL-60 cells was examined. aipA mRNA levels were approximately 10- to 20-fold higher between 30 and 36 hours - time points that correspond to RC-to-DC transition, exit, and reinfection - than between 4 and 26 hours, time points that correspond to conversion to and replication of non-infectious RC organisms (Troese et al, 2009) (Figure 2A). Also, the aipA mRNA level of the DC inoculum was comparable to that detected at 36 h, a time point that correlates with reinfection.
Next, whether or not the differential aipA transcription pattern observed in infected HL- 60 cells correlated with differential AipA protein expression was examined. AipA amino acids 1 - 87 (AipAi-87), 165-204 (AipA[65-204) and 249-355 (A1PA249-355) contain segments that are hydrophilic and predicted to be accessible on the protein's surface (Figure IB). AipAi.87 and AipAi65-204 are predicted to be exposed on the bacterial surface, while AipA24 -3 5 is not (Figure IB). AipAi-87 and AipA249-355 were expressed in Escherichia coli as proteins N-terminally fused to glutathione-5-transferase. Despite numerous attempts, soluble GST-tagged full-length AipA and AipAi65-2o4 could not be expressed (data not shown). Mouse antiserum raised against AipAj. 87 and AipA249-355 fusion proteins recognized a band of the expected size in lysates of A.
phagocytophilum infected but not uninfected HL-60 cells (Figure 2B). Anti-AipA249-355 recognized an additional band having an apparent mobility that was slightly smaller than 75 kDa, suggesting that AipA may dimerize. Alternatively, anti-AipA249-355 may recognize an epitope that is shared with or is similar in sequence to that of the unknown A. phagocytophilum protein. An AipAi-87 antibody was used to screen infected HL-60 cells by immunofluorescence microscopy. Consistent with the transcriptional data, approximately 70% and 96% of the A. phagocytophilum inclusions contained AipA-expressing bacteria at 28 and 32 h, respectively, whereas considerably fewer inclusions were AipA-positive at earlier time points (Figure 2C). These data demonstrate that AipA is both transcriptionally and translationally upregulated during periods when A. phagocytophilum converts to and is in its infectious DC morphotype.
A. phagocytophilum genes that are induced during the tick transmission bloodmeal, such as ompA and aspl4, encode proteins that are important for establishing infection in mammalian hosts (Ojogun et al, 2012; Kahlon et al., 2013). Therefore, aipA expression in the salivary glands of A. phagocytophilum infected I. scapularis nymphs was examined over the course of transmission feeding on na'ive mice. aipA mRNA was undetectable in unfed infected ticks (Figure 2D). However, aipA transcripts were significantly induced at 24 h of tick feeding and were increasingly expressed through to 72 h. To ensure that A. phagocytophilum transcription was not globally upregulated during tick transmission feeding, groEL (aph0240) expression was also examined. groEL was detected at the highest level in infected unfed nymphs and decreased in expression over the duration of the bloodmeal. Thus, A. phagocytophihim specifically induces select genes, including aipA, as it adapts during the transmission bloodmeal to colonize the mammalian host.
Consistent with a prior report that A. phagocytophihim preferentially expresses aipA during growth in HL-60 cells versus I. scapidaris embryo-derived ISE6 cells (Nelson et al., 2008), AipAi_87 antibody failed to detect a protein of the expected size in lysates of ISE6 cells in which A. phagocytophihim had been continually passaged for one, six, or seven weeks (Figure 2E). APH0032, which is an A. phagocytophihim protein that was previously demonstrated to be expressed during infection of ISE6 cells (Huang et al., 2010b), was detected in all infected samples. HGA patient serum and serum from a dog that had been naturally infected with A. phagocytophihim each detected GST-AipAi-87 (Figure 2F), confirming that AipA is expressed and elicits a humoral immune response during A. phagocytophihim infection of humans and dogs. Two additional HGA patient sera recognized GST-AipAi-87 (data not shown).
AipA is an A. phagocytophilum surface protein
To confirm whether AipA localizes to the bacterial outer membrane, confocal microscopy was used to screen infected RF/6A endothelial cells with AipAi_87 antibody in conjunction with antiserum targeting the A. phagocytophilum major surface protein, Msp2 (P44) (Carlyon, 2012). Both antibodies detected intravacuolar organisms, yielding the ring-like staining pattern on their peripheries that is characteristic for Msp2 (P44) and other confirmed A. phagocytophilum OMPs (Ge et al, 2007; Ojogun et al, 2012; Kahlon et al, 2013) (Figure 3A). Msp2 (P44) signal colocalized with and extended beyond the AipA signal. Next, to determine if immunoaccessible AipA domains are exposed on the bacterial surface, the surfaces of intact, host cell-free DC bacteria were treated with trypsin, an approach that has been used to confirm surface localization of A. phagocytophilum and Chlamydia trachomatis OMPs (Wang et al, 2006 ; Ojogun et al, 2012; Kahlon et al, 2013). AipA residues 1 to 87 include six lysine and three arginine residues, making this putative surface exposed region susceptible to tryptic digest. If AipAi-87 or portions thereof were exposed on the A. phagocytophilum surface, then incubating intact bacteria with trypsin should result in proteolytic cleavage of this region of the protein, which, in turn, would result in an inability to detect AipA. Trypsin-treated DC organisms were solubilized, Western-blotted, and probed with antiserum specific for AipAi-87 or a confirmed surface-exposed epitope of Asp55 (55-kDa phagocytophilum surface protein) (Ge et αί, 2007). Blots were also probed with antiserum targeting APH0032, which does not localize to the A. phagocytophilum outer membrane (Huang et al, 2010b). After surface trypsinolysis, APH0032 was detected but AipA and Asp55 were not (Figure 3B). Thus, AipA residues 1 to 87 are exposed on the A. phagocytophilum surface.
GST-AipA requires amino acids 1 to 87 to bind and competitively inhibit A. phagocytophilum infection of mammalian host cells
Since AipA is an exposed surface protein that is induced during key infection stages in the A. phagocytophilum life cycle, its ability to facilitate interactions with mammalian host cell surfaces to promote infection was investigated. It was assessed if GST-tagged AipA(-87 and AipA249-355 bind to RF/6A cells. GST alone served as a negative control. GST antibody detected GST-AipAi-87 that had adhered to the host cells by both immunofluorescence microscopy and flow cytometry (Figure 4, A and B). GST-AipA249-355 bound poorly, at best, and GST alone did not bind to host cells. Based on their differential adhesion capabilities, it was rationalized that GST-AipAi_87 but not GST-AipA249-355 would be able to serve as a competitive agonist to inhibit A. phagocytophilum infection. Indeed, preincubating HL-60 and RF/6A cells with GST-AipA|-87 significantly reduced the percentages of infected cells and the number of bacterial inclusions per cell by nearly four-fold relative to incubation with GST alone (Figure 4, C to F). In contrast, GST-AipA249.355 did not inhibit A. phagocytophilum infection of HL-60 cells and reduced infection of RF/6A cells by only a small degree. These data suggest that AipA residues 1 to 87 contain a domain that contributes to A. phagocytophilum invasion of myeloid and endothelial cells.
Antiserum targeting AipA residues 1 to 87 inhibits A. phagocytophilum infection of host cells Given that AipA amino acids 1 to 87 are exposed on the A. phagocytophilum surface and contribute to infection, it was assessed if treating DC organisms with heat-inactivated AipAi-87 antiserum prior to incubating them with HL-60 cells would inhibit infection. OmpA antiserum, for which its ability to inhibit A. phagocytophilum infection was previously validated (Ojogun et al, 2012), was a positive control. Anti-AipAi-87 and anti-OmpA each reduced the number of infected cells and the number of bacterial inclusions per cell by approximately 40% (Figure 5, A and B). In contrast, AipA249-355 antiserum had no effect on A. phagocytophilum infection. Consistent with the studies of A. phagocytophilum invasins (Ojogun et al, 2012; Kahlon et al, 2013), neither AipAi-87 nor OmpA antiserum inhibited bacterial adhesion to HL-60 cells (data not shown).
AipA targets a Le^ -capped PSGL-1 -independent receptor
sLex-capped PSGL-1 is the only known A. phagocytophilum receptor on myeloid host cells (Herron et al, 2000), and OmpA binds the 2,3-sialic acid determinant of sLex (Ojogun et al, 2012). Since A. phagocytophilum interactions with sLex-capped PSGL-1 involve at least one bacterial surface protein in addition to OmpA (Carl yon et al, 2003; Yago et al, 2003; Reneer et al, 2006; Sarkar et al, 2007; Reneer et al, 2008; Ojogun et al, 2012), it was investigated if AipAi„87 antiserum could inhibit bacterial binding to Chinese hamster ovary (CHO) cells transfected to express sLex-capped PSGL-1 (PSGL-1 CHO cells). These cells are excellent models for studying phagocytophilum interactions with sLe -capped PSGL-1 as they, but not untransfected CHO cells that do not express the receptor, support bacterial binding (Carlyon et al, 2003; Xia et al, 2003; Yago et al, 2003; Reneer et al, 2006; Sarkar et al, 2007; Reneer et al, 2008; Troese et al, 2009). DC organisms were incubated with AipAi-87 or AipA2 9-355 antiserum prior to being added to PSGL-1 CHO cells. Bacteria pretreated with preimmune serum were a negative control, whereas bacteria pretreated with OmpA antiserum served as a positive control. Additional positive controls for blocking A. phagocytophilum adhesion were PSGL-1 CHO cells that had been pretreated with KPL-1 or CSLEX1, which are monoclonal antibodies that block the bacterium's access to the PSGL-1 N-terminus or the a2,3-linked sialic acid determinant of sLex, respectively (Goodman et al, 1999; Herron et al, 2000; Troese et al, 2009). Incubating DC organisms with OmpA antibody and incubating PSGL-1 CHO cells with KPL-1 or CSLEX1 significantly reduced the numbers of bound DC organisms by two- to threefold (Figure 5C). A. phagocytophilum bound poorly to untransfected CHO cells. Preimmune serum, anti-AipAi-87, and anti-AipA249-355 failed to inhibit bacterial binding to PSGL-1 CHO cells. Therefore, AipA contributes to A. phagocytophilum cellular invasion by interacting with an sLex-capped PSGL-1 -independent receptor.
A combination of antisera targeting AipA, OmpA, and Aspl4 blocks A. phagocytophilum infection of host cells
A. phagocytophilum infection requires cooperative interactions of multiple invasins with the host cell surface (Truchan et al, 2013). Incubating DC organisms with antiserum targeting full-length OmpA (Ojogun et al, 2012), full-length Aspl4 (Kahlon et al, 2013), or AipAi-87 significantly, but only partially, reduced A. phagocytophilum infection of mammalian host cells. It was therefore investigated if blocking multiple bacterial -host interactions by treating DC organisms with combinations of AipAi-g7, OmpA, and/or Aspl4 antisera could improve blocking efficacy. The result was synergistic: whereas antisera combinations targeting two of the three invasins together more effectively inhibited infection than an antiserum targeting an individual protein, the most effective blocking of A. phagocytophilum infection was achieved using antisera against all three invasins (Figure 6). These data demonstrate that simultaneously targeting AipA, OmpA, and Asp 14 is an effective means for preventing or treating A. phagocytophilum infection. The AipA invasion domain is contained within residues 9 to 21
It was next sought to pinpoint the AipA invasion domain. The competitive agonist and antisera blocking studies indicated that this domain lies within residues 1 to 87. Based on hydrophobicity and surface probability analyses (Figure IB), it was rationalized that, of the AipA region of interest, amino acids 9 to 21 and/or 61 to 84 were most likely to facilitate interactions with host cells that promote infection. Accordingly, rabbit antisera against each of these peptides was generated for use in antibody blocking experiments. The AipA region encompassed by residues 165 to 204 (AipAi65_204) is predicted to be exposed on the A.
phagocytophilum outer membrane, hydrophilic, and accessible on the surface of AipA (Figure IB). Yet, the contribution of AipAi65-2o4 to infection was unknown due to our inability to express it as a soluble recombinant protein. Therefore, antisera to peptides corresponding to AipA amino acids 165 to 182 and 183 to 201 was also raised. Each AipA peptide antiserum recognized endogenous AipA in lysates of A. phagocytophilum infected, but not uninfected HL-60 cells and was specific for the peptide against which it was raised (Figure 7, A and B). Incubating DC organisms with AipAg.n antiserum reduced infection of HL-60 cells by approximately 47% relative to preimmune control serum, an inhibitory effect that was analogous to the reduction achieved using antiserum against AipAi-87 or OmpA (Figure 7C). Antisera against each of the other three AipA peptides and AipA249-355 had minimal or no inhibitory effect on infection. Thus, the AipA invasion domain is contained within residues 9 to 21.
DISCUSSION
Invasins of obligate intracellular pathogens are dualistic: they are essential for bacterial entry into host cells but, as such, they are also "Achilles' Heels" that can be blocked to prevent infection and pathogen survival. Targeting invasins of arthropod-transmitted pathogens that are induced during the arthropod blood meal is an attractive approach because of its ability to prevent not only establishment of infection but also disease transmission. Given that A.
phagocytophilum infection is predicated on the cooperative actions of multiple bacterial surface- associated invasins (Truchan et al, 2013), most of which are induced during the tick
transmission bloodmeal (Ojogun et al, 2012; Kahlon et al, 2013), effective prophylaxis against granulocytic anaplasmosis can be achieved by identifying and targeting these invasins. AipA is an attractive target to include in a multicomponent granulocytic anaplasmosis vaccine. It is an invasin that is induced during tick transmission feeding, is preferentially expressed during the bacterium's infectious stage, and functions synergistically with OmpA and Asp 14 to mediate optimal infection of mammalian host cells.
The exposure of AipA on the infectious DC form surface makes it accessible to blocking antibodies. Indeed, preincubating DC organisms with AipA antiserum significantly reduced infection of HL-60 cells. Pretreatment of DC bacteria with a combination of antibodies targeting AipA, OmpA, and Asp 14 nearly abolished infection, whereas pretreatment with antibodies against one or two of the three invasins was less effective. Thus, AipA, OmpA, and Asp 14 are collectively critical for infection and targeting all three together blocks infection in vitro. Spotted fever group Rickettsia species, which are in the Order Rickettsiales with A. phagocytophilum, also use multiple surface proteins to promote entry into host cells (Martinez et al., 2004;
Cardwell et al, 2009; Chan et al, 2009; Chan et al, 2010; Riley et al, 2010). Moreover, this pathogenic strategy is common among numerous other Gram negative bacterial pathogens, including Chlamydia pneumoniae (Molleken et al, 2010; Molleken et al., 2013), Legionella pneumophila (Garduno et al, 1998; Stone et al., 1998; Cirillo et al, 2001 ; Chang et al., 2005; Vandersmissen et al, 2010; Duncan et al, 2011), Bordetella pertussis (Brennan et al, 1996), Haemophilus influenzae (Jurcisek et al, 2007; Giufre et al, 2008; Chang et al, 201 1 ; Dicko et al, 201 1 ; Jalalvand et al, 2013; Singh et al., 2013) and Leptospira species (Barbosa et al, 2006; Pinne et al, 2010; Verma et al, 2010; Zhang et al, 2012).
GST- AipA is capable of binding to mammalian cells, which suggests that it functions not only as an invasin but also as an adhesin. Yet, AipA antibodies and GST-AipAi-87 each failed to inhibit A. phagocytophilum binding to mammalian cells. In these experiments, the role of AipA as an adhesin was presumably masked by the presence of other adhesins/invasins, such as OmpA and Aspl4, on the bacterial surface (Ojogun et al, 2012; Kahlon et al, 2013). The AipA receptor is unknown. However, because AipA antibody failed to inhibit A. phagocytophilum binding to PSGL-1 CHO cells, it can be concluded that AipA recognizes a sLex-capped PSGL-1 independent receptor. AipA and Asp 14, which also engages a sLex-capped PSGL-1 independent receptor (Kahlon et al, 2013), complement the sLex-targeting activity of OmpA (Ojogun et al, 2012).
Bacterial genes that are upregulated during transmission feeding of arthropod vectors are critical for various vector-transmitted bacteria to establish infection in their mammalian hosts (Hinnebusch et al, 1996; Perry et al, 1997; Grimm et al, 2004; Tilly et al, 2006). Consistent with these phenomena, aipA is not expressed by phagocytophilum during its residence in ISE6 cells or I. scapularis nymphs, but is induced when the bacterium is cultivated in mammalian tissue culture cells and during tick transmission feeding. Furthermore, A. phagocytophilum expresses AipA during infection of humans and dogs. Thus, AipA is dispensable for bacterial colonization of the tick vector, but is important for infecting mammalian hosts. Similar expression profiles have been observed for the other identified invasins OmpA, Asp 14, and APH1235 (Mastronunzio et al, 2012; Ojogun et al, 2012; Kahlon et al, 2013). Also, like APH1235 (Troese et al, 201 1 ; Mastronunzio et al, 2012), AipA is pronouncedly upregulated when the bacterium is in the DC stage. In agreement with the invasive role of the DC
morphotype, both proteins are important for establishing and/or maintaining infection in mammalian host cells.
How AipA is transported to and associates with the bacterial outer membrane is unclear, as it lacks a canonical signal peptide that would target it for Sec-dependent or twin-arginine secretion. This conundrum is further complicated as AipA is unique to A. phagocytophilum and bears no homology to any known crystal structure. Perhaps AipA is an atypical transmembrane protein or a peripheral membrane protein that is anchored to the bacterial outer membrane via a posttranslational modification. AipA colocalizes with the confirmed outer membrane protein, Msp2 (P44) and functions in concert with OmpA and Asp 14, both of which have also been shown to colocalize with Msp2 (P44) (Kahlon et al, 2013; Ojogun et al, 2012). Msp2 (P44) has been proposed to form heteromeric complexes that mediate interactions with host cells (Park et al, 2003). Given that AipA, Aspl4, and OmpA synergistically promote A. phagocytophilum infection of host cells, it will be important to determine if they do so as a multimeric invasin complex that includes Msp2 (P44).
The AipA invasion domain lies within residues 9 to 21, which is a hydrophilic region of the protein that is exposed on the bacterial surface. Antiserum targeting this span reduces A. phagocytophilum infection of host cells by a level comparable to that achieved by antiserum targeting the entire surface-exposed N-terminal domain of AipA. The observed inhibition is specific to anti-AipA9-2i, as antisera targeting peptides corresponding to all other predicted hydrophilic regions of AipA exhibited no inhibitory effect. Previously, it was discovered that the N-terminal ectodomain of OmpA is required for recognition of al, 3 -linked sialic acid of sLex (Ojogun et al., 2012) and the Asp 14 invasion domain lies within residues 101 -124 (Kahlon et al, 2013).
Granulocytic anaplasmosis can be debilitating or fatal, and there is no vaccine that protects against the disease. The results described herein show for the first time that
simultaneously targeting multiple A. phagocytophilum invasins effectively blocks infection in vitro.
EXAMPLE 2. Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells.
SUMMARY
Anaplasma phagocytophilum causes granulocytic anaplasmosis, an emerging disease of humans and domestic animals. The obligate intracellular bacterium uses its invasins OmpA, Asp 14, and AipA to infect myeloid and non-phagocytic cells. The present study identifies the OmpA binding domain as residues 59 to 74. Polyclonal antibody generated against a peptide spanning OmpA residues 59 to 74 inhibited A. phagocytophilum infection of host cells and binding to its receptor, sialyl Lewis x (sLex)-capped P-selectin glycoprotein ligand 1. Molecular docking analyses predicted that OmpA residues G61 and K64 interact with the two sLex sugars that are important for infection, a2,3-sialic acid and al,3-fucose. Amino acid substitution analyses demonstrated that K64 was necessary and G61 was contributory for recombinant OmpA to bind to host cells and competitively inhibit A. phagocytophilum infection. Adherence of OmpA to RF/6A endothelial cells, which express little to no sLex but express the structurally similar glycan, 6-sulfo-sLe , required al, 3 -sialic acid and al,3-fucose and was antagonized by 6- sulfo-sLex antibody. Binding and uptake of OmpA-coated latex beads by myeloid cells was sensitive to sialidase, fucosidase, and sLex antibody. The Asp 14 binding domain was also defined, as antibody specific for residues 113 to 124 inhibited infection. An antibody cocktail targeting the OmpA, Asp 14, and AipA binding domains neutralized A. phagocytophilum binding and infection of host cells. This study dissects OmpA-receptor interactions and demonstrates the effectiveness of binding domain-specific antibodies for blocking phagocytophilum infection. INTRODUCTION
Human granulocytic anaplasmosis (HGA) is an emerging tick-borne zoonosis in the United States, Europe, and Asia (Truchan et al., 2013). The number of HGA cases reported to the U. S. Centers for Disease Control and Prevention rose nearly seven-fold between 2003 and 2012 (CDC 2013; Hopkins et al., 2005). Seroprevalence data indicate that the disease is underreported in some endemic regions (Hao et al, 2013; Zhang et al., 2012; Zhang et al., 2009; Aguero et al, 2002; Bakken et al., 1998). HGA can also be spread via perinatal, nosocomial, and blood transfusion routes (Bakken et al, 1998; Alhumaidan et al., 2013; Annen et al, 2012; Jereb et al., 2012). It is an acute illness characterized by fever, chills, headache, malaise, leukopenia, thrombocytopenia, and elevated liver enzymes. Complications can include shock, seizures, pneumonitis, rhabdomyolysis, hemorrhage, increased susceptibility to secondary infections, and death. Risk for complications and fatality is greater for the elderly, the immunocompromised, and when proper diagnosis and/or antibiotic therapy are delayed (Truchan et al., 2013). The causative agent of HGA is Anaplasma phagocytophilum, an obligate intracellular bacterium that exhibits a tropism for neutrophils (Truchan et al., 2013). A. phagocytophilum is carried by a variety of wild animal reservoirs and, in addition to humans, causes disease in domestic animals including dogs, cats, horses, and sheep (Stuen et ai, 2013).
A. phagocytophilum exhibits a biphasic developmental cycle similar to that of Chlamydia spp., Ehrlichia spp., and Coxiella burnetii (Bastidas et al., 2013; Minnick et al., 2012; Zhang et al., 2007; Troese et al., 2009). The A. phagocytophilum infectious dense-cored (DC) form promotes its receptor-mediated uptake into a host cell derived vacuole. Within its vacuole, the DC develops into the non-infectious reticulate cell (RC) form that replicates to form a bacterial cluster called a morula (Troese et ah, 2009; Ojogun et al., 2012). RCs then convert back to DCs and are released to initiate the next infection cycle (Troese et al., 2009).
Sialyl Lewis x ([NeuAca(2-3)Gai i-4(Fucal-3)GlcNac]; sLex), an a2,3-5 sialylated and al ,3-fucosylated core-2 O-linked glycan that caps the N-termini of selectin ligands (Sperandio et al., 2006), is a critical A. phagocytophilum receptor (Goodman et al., 1999). sLex is richly expressed on mammalian cells that are permissive for A. phagocytophilum infection such as neutrophils, bone marrow progenitors, and promyelocytic HL-60 cells ( arakantza et al, 1994; Symington et al., 1985; Fukuda et al., 1984). A. phagocytophilum recognizes sLex that caps the N-terminus of P-selectin glycoprotein ligand-1 (PSGL-1) on these myeloid cells (Goodman et al, 1999; Herron et al., 2000). Neutrophils and HL-60 cells that have been treated with an sLex blocking antibody, from which surface sialic acids have been enzymatically removed, or that are devoid of sialyltransferase and/or l ,3-fucosyltransferase activity are resistant to A.
phagocytophilum binding and infection (Ojugun et al., 2012; Goodman et al., 1999; Carylon et al., 2003; Yago et al, 2003). A.phagocytophilum also infects rhesus monkey choroidal (RF/6A) endothelial cells, megakaryoblastic MEG-01 cells, and bone marrow-derived mast cells in tissue culture. Infection of these non-myeloid host cell types depends on sLex itself, 2,3-sialic acid, and/or l,3-fucose (Huang et al., 2012; Kahlon et al., 2013; Mastronunzio et al., 2012; Ojogun et al, 201 1). Thus, sLex and possibly other closely related a2,3-sialylated and ctl,3-fucosylated molecules are essential for efficient A phagocytophilum infection of mammalian cells.
A. phagocytophilum OmpA and a2,3-sialic acid (N-acetylneuraminic acid [Neu5Ac], further referred to as sialic acid throughout) was identified as the bacterium's first
adhesin/invasin-receptor pair (Ojogun et al., 2012). OmpA binding to the a2,3-sialic acid determinant of sLex on myeloid cells and to a2,3-sialylated glycans on RF/6A cells are vital steps in A. phagocytophilum invasion of these host cell types (Ojogun et al., 2012). Exposure of OmpA on the A. phagocytophilum DC surface makes it accessible to antibodies (Ojogun et al, 2012), which could be used to exploit the bacterium's obligatory intracellular nature to block the host cell invasion step that is essential for survival. The OmpA binding domain that recognizes a2,3-sialic acid lies within amino acids 19 to 74 (Ojogun et al., 2012), but has yet to be specifically identified. The phagocytophilum OMP that recognizes l,3-fucose is unknown. As shown in Example 1, OmpA functions in concert with two additional invasins that are also upregulated during tick transmission feeding, Asp 14 (14-kDa A phagocytophilum surface protein) and AipA A. phagocytophilum invasion protein A), to promote optimal A.
phagocytophilum entry into mammalian host cells (Kahlon et al., 2013; Seidman et al., 2014). The AipA binding domain was defined in Example 1 as residues 9 to 21.
In this study, antibody blocking, in silico docking models, and site directed mutagenesis was used to identify the A. phagocytophilum OmpA binding domain, specifically the key residues that are essential for its adhesin/invasin activity, and determined that it recognizes both a2,3-sialic acid and al,3-fucose. This work represents the most detailed study of any rickettsial adhesin/invasin-receptor pair to date. Furthermore, the Asp 14 binding domain was identified and it was confirmed that an antibody cocktail targeting the binding domains of OmpA, Asp 14, and AipA nearly abolishes A. phagocytophilum infection of host cells.
MATERIALS AND METHODS
Cell lines and cultivation of A. phagocytophilum
Uninfected and A. phagocytophilum infected (NCH-1 strain) HL-60 cells (ATCC CCL- 240) and RF/6A cells (ATCC CRL-1790, Manassas, VA) were maintained as previously described (Troese et al, 2009; Huang et al, 2012). CHO (-) and PSGL-1 CHO cells were cultivated as described (Li et al., 1996).
Site directed mutagenesis and recombinant proteins
pGST-OmpA, which encodes OmpAl 9-205 N-terminally fused to GST, was previously constructed (Ojogun et al, 2012). Using pGST-OmpA as the template, the QuikChange®
Lightning (Agilent Technologies, Santa Clara, CA) protocol was used per the manufacturer's guidelines to perform site-directed insertions and point mutagenesis of the ompA insert sequence. For site directed insertions, a five-amino acid insert sequence (CLNHL; SEQ ID NO:75) was selected based on previous studies that had successfully employed the linker-scanning method (Anton et al, 2004; Okoye et al, 2006), which is used to insert peptide "linkers" to disrupt protein binding domains without perturbing overall protein structure. The sequence chosen for the insertion peptide, CLNHL (SEQ ID NO:75), was a consensus sequence based on the most common amino acids at their respective positions in the insertion peptides used in prior studies (Anton et al, 2004; Okoye et al, 2006). The nucleotide sequence, 5'-TGCCTGAACCACCTG-3' (SEQ ID NO:76), which encoded CLNHL (SEQ ID NO:75), was inserted in the ompA sequence of pGST-OmpA between ompA nucleotides 102 and 103, 162 and 163, 186 and 187, 201 and 202, 216 and 217, and 231 and 232 to yield plasmids that encoded GST-OmpA proteins bearing CLNHL inserts between OmpA amino acids 34 and 35, 54 and 55, 62 and 63, 67 and 68, 72 and 73, and 77 and 78, respectively. Likewise, the QuikChange protocol was used to perform site directed mutagenesis to yield plasmids that encoded GST-OmpA proteins having R32, D53, K60, G61, K64, K65, E69, and/or E72 converted to alanine. GST-OmpA mutants were expressed and purified as previously described (Ojogun et al, 2012). Plasmids encoding His- tagged wild type and site-directed mutant OmpA proteins were generated by amplifying wild type and mutant ompA sequences using primers containing ligase-independent cloning (LIC) tails and annealing the amplicons into the pET46 Ek/LIC vector (Novagen, EMD Millipore, Darmstadt, DE) per the manufacturer's instructions. His-OmpA proteins were expressed and purified by immobilized metal-affinity chromatography as previously described (Miller et ai, 2011).
Molecular modeling of the OmpA-sLex interaction
To obtain a putative three-dimensional OmpA protein structure, the mature OmpA sequence was threaded onto the solved crystal structures of proteins with similar sequences using the PHYRE2 server as previously described (Ojogun et ah, 2012). Amino acids 19 to 150 (73% of the mature OmpA sequence) were modeled with greater than 90% confidence to known structures for similar proteins (Protein Data Bank [PDB] files 2aiz {Haemophilus influenzae OmpP6 peptidoglycan associated lipoprotein (PAL)], 4g4v [Acinetobacter baumanni PAL], 4b5c [Burkholderia pseudomallei PAL], 31dt [Legionella pneumophila OmpA], 2kgw [Mycobaterium tuberculosis OmpATb]). The remainder of the protein lacked sufficient homology to any experimentally derived structure, but could be modeled using the Poing method (Kelley et ai, 2009), which was performed as part of the Phyre2 analyses. The sLex-PSGL-lpeptide (residues 61 to 77) and the sLex glycan itself was extracted from the solved crystal structure of PSGL-1 (PDB 1G1 S) in PyMol and saved as an individual PDB file. Open Babel software was used to convert PDB files to PDBQT (Protein Data Bank, Partial Charge and Atom Type) format in order to perform OmpAsLe" docking analysis. AutoDock Tools software was used to generate the docking output files for both the OmpA protein structure and the sLex ligand. The search location for OmpA was generated in AutoDock Tools by setting a search grid that encompassed OmpA residues 19 to 74. Molecular docking was performed using AutoDock Vina to identify potential points of interaction between OmpA and sLex. The top two OmpA-sLe models generated by AutoDock Vina had the same predicted affinity value of -4.2 kcal/mol and were selected for analysis in PyMol to determine potential points of contact.
Antibodies, reagents, enzyme-linked immunosorbent assay (ELISA), and Western blotting
To generate antisera specific to the OmpA and Asp 14 binding domains, peptides corresponding to OmpA residues 23 to 40, 41 to 58, and 59 to 74 and Aspl4 residues 98 to 112 and 1 13 to 124 were synthesized, conjugated to keyhole limpet hemocyanin, administered to rabbits, and the resulting OmpA and Asp 14 peptide-specific sera were affinity-purified by New England Peptide (Gardner, MA). Each peptide antiserum's specificity for the peptide against which it had been raised and for its protein target was determined by ELISA using the TMB substrate kit (Thermo Scientific, Waltham, MA) following the manufacturer's instructions or by Western blot analysis as previously described (Carylon et al., 2002). Mouse anti-AipA peptide antisera have been previously described (Seidman et al., 2014). sLex 564 antibodies CSLEX1 (BD Biosciences, San Jose, CA) and KM93 (Millipore, Darmstadt, DE) and PSGL-1 N- terminus-specific antibody 565 KPL-1 (BD Biosciences) were obtained commercially. Fab fragments of OmpA and Asp 14 peptide-specific antisera were generated using the Fab
Preparation Kit (Pierce, Rockford, IL) according to the manufacturer's instructions. His tag and Alexa Fluor 488-conjugated secondary antibodies and Alexa Fluor 488-conjugated streptavidin were obtained from Invitrogen (Carlsbad, CA). Biotinylated AAL and MAL II were obtained from Vector Labs (Burlingame, CA). Glycosidases used in this study were a2,3/6- sialidase (Sigma-Aldrich, St. Louis, MO) and al ,3/4-fucosidase (Clontech, Mountain View, CA).
Sequence alignments
The NCH-1 gene sequence for ompA (APH0338) was previously determined (Ojogun et al., 2012; Kahlon et al., 2013; Seidman et al., 2014). A Protein BLAST (basic local alignment search tool) search using the NCH-1 OmpA predicted protein sequence as the query was used to identify homologs in other Anaplasmataceae species and in A. phagocytophilum strains HZ (Rikihisa et al, 1997), HGE1 (Goodman et al., 1996), Dog (Al-Khedery et al., 2012), JM
(Johnson et al, 2011), MRK (Madigan et al, 1987; Gribble, 1969), CRT35, CRT38 (Massung et al., 2007), and NorV2 (Al-Khedery et al., 2012), for which the genomes are available. All of these strains except for NorV2 had been originally isolated from clinically affected humans and animals. HZ and HGE1 were recovered from human patients in Westchester, NY, USA and Minnesota, USA, respectively. The Dog and JM strains were isolated from a dog in Minnesota, USA and a meadow jumping mouse (Zapus hudsonius) in Camp Ripley, MN, USA. MRK had been recovered from a horse in California, USA. CRT35 and CRT38 are isolates of the A phagocytophilum
Figure imgf000061_0001
1 strain that were recovered from ticks collected at Camp Ripley, MN, USA. NorV2 is a naturally occurring A. phagocytophilum isolate that was maintained in an experimentally infected lamb, exhibits reduced virulence in sheep, and differs in its 16S rRNA gene sequence when compared to other sheep isolates. OmpA sequence alignments were generated using Clustal W.
Binding of recombinant OmpA proteins to host cells
For binding of His- or GST-tagged OmpA proteins to host cells, RF/6A or HL-60 cells were incubated with 4 μΜ recombinant protein in culture media for 1 h in a 37°C incubator supplemented with 5% C02 and a humidified atmosphere. To assess for the presence of sLex or 6-sulfo-sLex on RF/6A cell surfaces, the cells were fixed in 4% PFA in PBS for 1 h at room temperature followed by incubation with CSLEX1 , KM93, or G72 for 1 h at room temperature. Antibody incubations and washes were performed as described previously (Reneer et al., 2006). Spinning-disk confocal microscopy using an Olympus BX51 microscope affixed with a disk- spinning unit (Olympus, Center Valley, PA) and/or flow cytometry using a BD FACS Canto™ II (BD Biosciences) were performed to assess binding of antibodies or His-OmpA proteins to host cell surfaces as previously described (Ojogun et al, 2012; Kahlon et al, 2013). In some cases, RF/6A cells were pretreated with a2,3/6-sialidase, al,3/4-fucosidase, AAL, MAL II, or sLex- or 6-sulfo-sLex-specific antibodies prior to incubation with His-OmpA.
Competitive inhibition of A. phagocytophilum binding and infection
Competitive inhibition assays utilizing recombinant protein or antibody were performed and analyzed by spinning-disk confocal microscopy as previously described (Ojogun et al., 2012; Kahlon et al., 2013). To determine if A. phagocytophilum binding to PSGL-1 CHO cells or infection of RF/6A cells involved bacterial binding to host cell surface fucose residues, the host cells were treated with al,3/4-fucosidase (10 μυ/mL) prior to the addition of DC organisms and assessment for bacterial binding or infection as previously described (Troese et al., 2009; Ojogun et al., 2012). For competitive inhibition assays using antisera raised against OmpA or Asp 14 peptides, A. phagocytophilum DC bacteria were incubated with serially diluted concentrations of antiserum. Preimmune rabbit serum (200 μg/mL) was a negative control. Assays using combinations of two or three different OmpA, Asp 14, or AipA peptide antibodies were performed using 100 μg/mL per antibody. Preimmune serum (200 μg/mL or 300 μg/mL, based on the combined total of peptide antisera) served as a negative control. Competitive inhibition assays using OmpA and/or Asp 14 Fab fragments were performed exactly as described for antisera. Preimmune Fab fragments served as a negative control.
OmpA coated bead uptake assay 1.8 x 107 red fluorescent sulfate-modified 1.0 μηι diameter microfluorospheres (Life Technologies, Carlsbad, CA) were mixed by rotation with 8 μg of His-OmpA, or His-OmpA proteins bearing alanine substitutions, in 400 of 50 mM phosphate buffered saline (PBS) supplemented with 0.9% NaCl at room temperature overnight in the absence of light. The His- OmpA coated beads were centrifuged at 5,000 g for 25 min, followed by three washes in 50 mM PBS. Coated beads were resuspended in 400 μΐ, of 50 mM PBS, 0.9% NaCl, 1% BSA and stored at 4°C until use. To validate that the beads were conjugated with His-OmpA, 1.8x104 of the beads were screened by immunofluorescent microscopy using mouse polyclonal OmpA antisera followed by Alexa Fluor 488-conjugated goat anti-mouse IgG as described (Ojogun et al., 2012). To assess binding to and uptake by HL-60 or RF/6A cells, His-OmpA coated beads or uncoated control beads were resuspended in the appropriate culture medium and added to host cells at a concentration of 500 beads/cell. For adherent RF/6A cells, beads were centrifuged onto the host cells at 1,000 g for 5 min. The cells plus beads were incubated for 1 h at 37°C in a 5% C02 supplemented humidified incubator followed by washing the cells three times with PBS to remove unbound beads. Non-adherent HL-60 cells were mixed with the beads in suspension, incubated as described above, and three PBS washes were performed intermittently between five-min spins performed at 300 g. To assess binding, the host cells were fixed in 4%
paraformaldehyde (PFA) in PBS, mounted with Pro Long® Antifade Gold gel mounting medium containing 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen), and analyzed by spinning-disk confocal microscopy as previously described (Ojogun et al., 2012). For uptake assays, after the final wash, the host cells were resuspended in culture medium and cultivated for an additional 7 h. The cells were washed three times in PBS, incubated with a 0.25% trypsin solution
(Hyclone™, Thermo Scientific, Waltham, MA) forlO min at 37°C to cleave host cell surface proteins and consequently remove noninternalized beads, and washed three times with PBS. HL- 60 cells were cytospun onto glass microscope slides and fixed, mounted, and screened as described above. RF/6A cells were added to wells containing coverslips, incubated overnight in a 37°C incubator supplemented with 5% CO? and a humidified atmosphere to allow the host cells to adhere prior to further processing. To determine if His-OmpA coated bead binding or uptake was temperature sensitive, some experiments were performed at 4°C. To assess the contribution of sLex or PSGL-1 determinants to His-OmpA coated bead binding and uptake, host cells were pretreated with a2,3-sialidase (5 μg/mL), al ,3/4-fucosidase (ΙΟμυ/mL), sLex-specific antibody CSLEX1 (10 μ τηΐ,), PSGL-1 N-terminus-specific antibody KPL-1 (10 μg/mL), or vehicle or isotype controls as previously described (Ojogun et al., 2012) prior to the bead binding and uptake assays.
Scanning electron microscopy
Coverslips of RF/6A cells were incubated with OmpA coated or control beads as described above. The coverslips were fixed in 2.0% glutaraldehyde in 0.1 M sodium cacodylate for 1 h at room temperature. The coverslips were subjected to two 10-min washes in 0.1 M sodium cadodylate and fixed in 1.0% osmium tetroxide in 0.1 M sodium cacodylate for 1 h. The coverslips were rinsed two more times with 0.1 M sodium cadodylate buffer for 10 min each. The samples were dehydrated by successive 5 -min incubations in 50% ethanol, 70% ethanol, 80%) ethanol, 95% ethanol, and three 10-min washes in 10% ethanol. Next, the samples were incubated three times for 30 min each in hexamethyldisilazane, air-dried, mounted with silver paint, and sputter coated with gold before imaging on a Zeiss EVO® 50XVP scanning electron microscope (Thornwood, NY). For HL-60 cells incubated in suspension with beads, the samples were retained on a 0.1 μιη filter and processed exactly as described for RF/6A cells.
Statistical analyses
The Prism 5.0 software package 680 (Graphpad, San Diego, CA) was used to determine the statistical significance of data using one-way analysis of variance (ANOVA) or the Student's T-test, as previously described (Ojogun et al., 2012). Statistical significance was set to P < 0.05. RESULTS
OmpA amino acids 59 to 74 are critical for A. phagocytophilum to bind to sLex capped PSGL-1 and for infection of mammalian host cells
The OmpA region that is important for A. phagocytophilum infection of mammalian host cells lies within residues 19 to 74 (OmpA19-74) (Ojogun et al., 2012). As a first step in further delineating the binding domain, polyclonal antisera against peptides corresponding to OmpA amino acids 23 to 40, 41 to 58, and 59 to 74 were raised. It was verified that the antisera were specific for OmpA by confirming that each recognized recombinant forms of mature OmpA (minus the signal sequence; corresponding to residues 19 to 205 and hereafter referred to as OmpA) and OmpA19-74, but neither OmpA75-205 nor Aspl4 (Fig. 8A). Anti-OmpA41-58 and anti-OmpA59-74 were specific for their target peptides at all serum dilutions. Anti-OmpA23-40 was specific for its target peptide at most dilutions tested, but exhibited low level recognition of OmpA41-58 at dilutions below 1 : 12,800 (Fig. 8A). Next, it was evaluated if any of the OmpA peptide antisera could inhibit A. phagocytophilum infection of host cells. Bacteria that had been treated with anti-OmpA or preimmune serum served as positive and negative controls, respectively. As previously observed (Ojogun et al., 2012), OmpA antibody reduced the percentage of A. phagocytophilum infected HL-60 cells by approximately 40% (Fig. 8B).
OmpA59-74 antibody exhibited a dose-dependent inhibitory effect and, at a concentration of 200 ug/ml, reduced the percentage of infected HL-60 cells by approximately three-fold. Antisera targeting OmpA residues 23 to 40 and 41 to 58 exhibited very little to no inhibition of infection, regardless of concentration. Unless otherwise specified, all antisera were used at a concentration of 200 ug/ml in subsequent blocking experiments.
sLex-capped PSGL-1 is an A. phagocytophilum receptor on human myeloid cells
(Goodman et al., 1 99), and OmpA has been shown to bind the sLex portion (Ojogun et al., 2012). Because OmpA59-74antibody significantly inhibited A. phagocytophilum infection of HL-60 cells, it was rationalized that OmpA amino acids that are critical for engaging the receptor are within residues 59 to 74. To test this hypothesis, the abilities of antisera targeting various portions of OmpA to interfere with A. phagocytophilum binding to Chinese hamster ovary cells transfected to express sLex-capped PSGL-1 (PSGL-1 CHO cells) was assessed. These cells are useful models for studying A phagocytophilum interactions with sLex and/or PSGL-1 because they robustly support bacterial binding but not infection, while untransfected CHO [CHO (-)] cells that lack expression of these receptors poorly support bacterial binding. Anti-OmpA59-74 reduced the mean number of bound A. phagocytophilum DC organisms per PSGL-1 CHO cell by approximately fourfold to nearly that of CHO (-) cells (Fig. 8C). Anti-OmpA reduced bacterial binding to PSGL-1 CHO cells by approximately two-fold. Anti-OmpA23-40, anti-OmpA41-58, and preimmune serum had no effect. These results indicate that the OmpA binding domain lies within amino acids 59 to 74 and this region is important for A. phagocytophilum recognition of sLe -capped PSGL-1.
Molecular docking models of A. phagocytophilum OmpA-sLe* interactions suggest that residues within OmpA59-74 engage sLe"
To complement the antibody blocking experiments, molecular modeling and
docking was used to identify the OmpA amino acids that possibly contact sLex. First, a three- dimensional model of the invasin was generated. A crystal structure for A. phagocytophilum OmpA has yet to be determined, but an abundance of crystal structures for similar bacterial proteins have been determined. The Phyre2 (Protein Homology/ Analogy Recognition Server version 2.0) server, which predicts three dimensional structures for protein sequences and threads the predicted models on known crystal structures (Kelley et al., 2009), was used to generate a tertiary structure model for OmpA (Fig. 9A). The resulting homology model predicted that OmpA residues 59 to 74 form part of a surface-exposed alpha helix (Fig. 9A), which could interact with ligands. Surface electrostatic values calculated using the adaptive Poisson- Boltzmann solver (APBS) plugin for PyMOL indicated that OmpA amino acids 19 to 74 have an overall cationic surface charge. The rest of the modeled protein exhibits an overall anionic surface charge (Fig. 9C and D). These findings are consistent with prior observations that bacterial and viral proteins that interact with sLex and/or sialic acid do so at cationic surface patches (Chung et al., 2007; Hermans et al, 2012; Dormitzer et al., 2002; Stein et al, 1994; Varghese et al., 1992).
For docking predictions, the sLex glycan (Fig. 9B) was extracted from the crystal structure of sLex-capped PSGL-1 (DOI: 10.2210/pdblgls/PDB). Autodock Vina was used to predict how OmpA might interact with sLe\ The search grid encapsulated OmpA19-74 (Fig. 9A). The top two docking models, each with the same predicted affinity value of -4.2 kcal/mol, displayed similar interactions between sLex and the OmpA region encompassed by amino acids 59 to 74. In both models, K.64 of OmpA was predicted to bind the a2,3-sialic acid residue of sLex (Fig. 9E and F). G61 was also predicted to interact with sLex in both models, though it was predicted to bind a2,3-sialic acid in one model and al,3-fucose in the other. Lastly, K60 was predicted to bind the Bl,3-galactose residue of sLex in the docking model presented in Fig. 9F. Together, the in silico predictions and peptide antibody blocking results suggest that OmpA 9- 74 contains critical residues that interact with sLex to promote A. phagocytophilum infection of host cells.
OmpA is conserved among A. phagocytophilum strains and K64 is conserved among Anaplasmataceae OmpA proteins
Aligning the OmpA sequence from the A. phagocytophilum NCH-1 strain, which was originally isolated from a HGA patient in Nantucket, MA (Kolbert et al., 1997), with those encoded by geographically diverse A. phagocytophilum isolates that had been recovered from infected humans, animals, and ticks revealed that OmpA is highly conserved among these strains (Figure 10A and B). Eight of the nine sequences were identical. The OmpA of NorV2 Norwegian sheep isolate had only three amino acid differences, none of which were within the binding domain encompassed by residues 59 to 74. The high degree of OmpA sequence conservation further supports the invasin's importance to A. phagocytophilum pathobiology. Next NCH-1 OmpA residues 19 to 74 were aligned with corresponding regions of OmpA homologs from marginale and Ehrlichia spp., which are in the family A naplasmataceae with A. phagocytophilum and infect bovine erythrocytes and human and animal monocytes, respectively (Carylon et al, 2012; Mansueto et al, 2012; Suarez et al., 201 1). A.
phagocytophilum OmpA K64 that was predicted to potentially interact with sLex (Fig. 9, E and F), was the only binding domain residue that was conserved among all Anaplasmataceae OmpA regions examined (Fig. 10B). Additional residues within the A. phagocytophilum OmpA binding domain, including the other two predicted to interact with sLe , K60 and G61 (Fig. 9, E and F), were conserved among Anaplasma spp. but not Ehrlichia spp. OmpA proteins (Fig. 10B).
G61 and 64 are essential for recombinant OmpA to optimally bind to mammalian host cells and competitively inhibit A. phagocytophilum infection
Because A. phagocytophilum is an obligate intracellular bacterium, developing a knock out-complementation system for this organism has proved challenging and has not been described. Therefore, a series of alternative approaches was utilized to further functionally evaluate OmpA. Recombinant OmpA can be used as a competitive agonist to block A.
phagocytophilum access to its receptor and thereby inhibit infection. This phenomenon was exploited to further define the OmpA amino acids that are critical for receptor recognition and bacterial uptake by assessing the competitive agonist abilities of OmpA proteins having site- directed amino acid changes. This approach was built on the rationale that OmpA proteins in which the binding domain was disrupted would be unable to inhibit infection. First, OmpA proteins N-terminally fused to glutathione-S-transferase (GST) were generated, each of which had an insertion of the peptide CLNHL at one of six different sites within residues 19 to 78. This approach has been used in previous studies to disrupt proteins' binding domains without perturbing overall protein structure, and the insertion sequence that was devised for this purpose was a consensus of the insertion peptides used in those studies (Anton et al., 2004; Grande et al., 2007; Okoye et al, 2006). Incubating HL-60 cells with the positive control, GST-OmpA, prior to the addition of DC bacteria resulted in a significant reduction in the percentage of infected cells relative to GST alone (Fig. 1 1A). GST-Om A proteins carrying insertions between residues 67 and 68 and between 62 and 63 were completely and partially abrogated, respectively, in their abilities to inhibit A. phagocytophilum infection. GST-OmpA proteins bearing insertions at other sites were unaffected in their ability to inhibit infection.
Next the specific amino acids of GST-OmpA that were critical for it to inhibit A.
phagocytophilum infection were identified. The competitive agonist assay was repeated using GST-OmpA proteins in which select amino acids had been mutated to alanine (Fig. 10 and Fig. 1 1, B and C). Many of the targeted residues were within OmpA amino acids 59 to 74. R32 and D53 were selected because they lie outside of residues 59 to 74, and, accordingly, it was anticipated that substituting them would not alter OmpA function. GST-OmpAK64A was considerably reduced in its ability to inhibit A. phagocytophilum infection (Fig. 1 1 , B and C), thereby indicating that this highly conserved residue was critical for GST-OmpA to serve as a competitive agonist. K65, however, was dispensable for this function, as the blocking ability of GST-OmpAK65A was uncompromised and the blocking ability of GST-OmpAKK6465AA was no greater than that of GST-OmpAK64. GST-OmpAG61 A displayed a modest but significant decline in its competitive agonist ability. Replacement of both G61 and K64 with alanines yielded an additive effect that was greater than substituting either residue alone, as GST
OmpAGK6164AA was unable to inhibit infection. GST-OmpA proteins in which R32, D53, K60, E69 and E72 had been mutated to alanine were each unaffected in the ability to hinder infection.
Given that K64 and G61 are vital and contributory, respectively, to the ability of recombinant OmpA to competitively inhibit A. phagocytophilum infection, it was evaluated if these residues mediate binding to mammalian host cell surfaces. RF/6A and HL-60 cells were incubated with His-tagged OmpA proteins. After unbound proteins were washed away, bound proteins were detected by flow cytometry using a His-tag antibody. His-tagged OmpA and
OmpAD53A bound equally well to RF/6A cells (Fig. 1 ID). His-OmpAG61A bound poorly, His- OmpAK64A even more so, and His-OmpAGK6164AA could not bind to host cells. Collectively, these data are consistent with the invasin-receptor contacts predicted by the OmpA-sLex docking models and underscore the importance of OmpA K.64 and G61 to OmpA-receptor interactions. OmpA interacts with al,3-fucose on mammalian host cell surfaces al,3-fucose is critical for A. phagocytophilum to bind PSGL-l-modeled glycopeptides, to bind and invade human and murine myeloid cells, and to establish infection in laboratory mice. Consistent with these observations, PSGL-1 CHO cells that had been pretreated with al,3/4- fucosidase were approximately three-fold less permissive for A. phagocytophilum binding (Fig. 12A). Multiple lines of evidence led to the hypothesis that OmpA binds al,3-fucose. First, OmpA binds a2,3 -sialic acid, which is in close proximity to a 1,3 -fucose on sLex. Second, the docking model in Fig. 9E predicted that OmpA residues within the binding domain contact both a2,3-sialic acid and al ,3-fucose of sLex. Third, OmpA is important for A. phagocytophilum infection of not only myeloid, but also endothelial cells. Fourth, fucose residues are critical for the pathogen to invade RF/6A endothelial cells, as pretreatment of the host cells with al ,3/4- fucosidase made them significantly less permissive to A. phagocytophilum binding (Fig. 12B) and infection (Fig. 12C).
To determine if OmpA recognizes fucose, His-tagged OmpA was incubated with RF/6A cells that had been treated with al ,3/4-fucosidase and binding was assessed by
immunofluorescence microscopy and flow cytometry. a2,3/6-sialidase-treated RF/6A cells were included as a positive control for a treatment that would make the host cells less permissive to recombinant OmpA binding. To verify the efficacy and specificity of both glycosidases, treated and untreated host cells were screened with AAL (Aleuria aurantia lectin) and MAL II (Maackia amurensis lectin II). AAL recognizes fucose residues that are in al,3- and l ,6-linkages with N- acetylglucosamine (Yamashita et al., 1985; Chandrasekaran et al., 2003). MAL II detects sialic acids that are in a2,3-linkages with galactose. Fucosidase treatment abolished AAL but not MAL II binding, while sialidase treatment eliminated MAL II but not AAL binding (Fig. 13, A to D). Thus, the glycosidases were effective and specific. His-OmpA binding to both sialidase- and fucosidase-treated RF/6A cells was comparablyreduced relative to vehicle control treated cells, while binding of His-Aspl4 was unaffected. Incubating the host cells with MAL II or AAL prior to the addition of His-OmpA competitively reduced the efficiency of His-OmpA binding by similar degrees as sialidase or fucosidase, respectively (Fig. 13E). Overall, these observations demonstrate that optimal adhesion of OmpA to host cells involves both oc2,3-sialic acid and al,3- fucose and that Asp 14 utilizes neither sialic acid nor fucose to bind to host cells.
OmpA interacts with 6-sulfo sLe* on RF/6A endothelial cell surfaces Because His-OmpA binding to RF/6A cells involved recognition of a2,3-sialic acid and al ,3-fucose (Fig. 13), it was hypothesized that OmpA interacts with sLe" or a sLe -like receptor on these host cells. sLex and the sLex-like molecule, 6-sulfo sLex (Neu5Ac(a2-3)Gal(pi- 4)[Fuc(al-3)][HS03(3-6)]GlcNAcl) (Fig. 14A) have both been detected on the surfaces of high endothelial venal, vascular, cancerous, and/or inflamed endothelial cells. To assess if either glycan is present on RF/6A cells, they were screened with sLex antibodies, CSLEX1 and KM93 and the 6-sulfo-sLex antibody, G72. Robust G72 signal but little to no CSLEX1 or KM93 signal was detected on RF/6A cells (Fig. 14, B and C). Binding of His-OmpA to RF/6A cells that had been pretreated with G72 was pronouncedly reduced relative to cells that had been incubated with CSLEX1 , KM93, or isotype control antibody (Fig. 14D). Thus, A.phagocytophilum OmpA recognizes 6-sulfo-sLex on RF/6A endothelial cells.
OmpA-coated beads bind to and are internalized by non-phagocytic endothelial cells
The ability of recombinant OmpA to bind to non-phagocytic RF/6A endothelial cells (Figs. 12-14), suggests that, in addition to functioning as an invasin, it may also exhibit adhesin activity. Furthermore, while OmpA on the A. phagocytophihim surface acts cooperatively with Asp 14 and AipA to mediate bacterial binding to and invasion of mammalian host cells, its ability to mediate these processes by itself is unknown. Therefore, the ability of recombinant OmpA to confer adhesiveness and invasiveness to inert particles was assessed. His-OmpA was coupled to fluorescent microspheres that were 1.0 μηι in diameter, a size similar to that of the diameter of an A. phagocytophihim DC organism (0.8 ± 0.2 μπι). Successful conjugation of His-OmpA to the beads was confirmed by immunofluorescence using OmpA antiserum (Fig. 15A). RF/6A cells were incubated with recombinant OmpA-coated or non-coated control beads and screened with OmpA antibody to determine the numbers of beads bound per cell. To assess bead uptake, the cells were incubated for an additional 1 to 8 h and trypsin was used to remove non-internalized beads prior to screening. Immunofluorescence microscopy revealed that significantly more OmpA coated beads bound to and were internalized by RF/6A cells versus control beads (Fig. 15, B and D). Scanning electron microscopy corroborated these results, as OmpA coated bead were observed bound to and inducing the formation of filopodia-like structures on the surfaces of RF/6A cells or covered by plasma membrane (Fig. 15C). Thus, OmpA alone was sufficient to mediate bead binding to and uptake by non-phagocytic RF/6A endothelial cells.
Binding and uptake of OmpA-coated beads by myeloid cells is dependent on sLe" Next the ability of His-OmpA coated beads to bind and enter HL-60 cells was assessed and, if so, whether these processes involve the OmpA myeloid cell receptor, sLe\ Scanning electron microscopy revealed that OmpA beads bound to and induced their own uptake into HL- 60 cells (Fig. 16A). Relative to the results obtained using RF/6A cells (Fig. l5D), OmpA coated bead binding to HL-60 cells was reduced (Fig. 16B). However, of the OmpA beads that did bind, approximately half of them were internalized (Fig. 16C). Approximately three- fold fewer control beads than OmpA coated beads bound to and were taken in (Fig. 16, B and C). OmpA bead uptake, but not adherence was pronouncedly inhibited when the assay was performed at 4°C versus 37°C. Beads coated with OmpAG61A, OmpAK64A, Omp AGK6164AA, and
OmpA K6465AA were significantly compromised in their abilities to bind to and be
internalized by HL-60 cells (Fig. 16, B and C). OmpA bead cellular adherence and entry were significantly inhibited and neutralized, respectively, for host cells that had been pretreated with 2,3/6-sialidase or al,3/4-fucosidase (Fig. 16, D and E). Moreover, the sLex-specific antibody, CSLEX1 significantly reduced binding and blocked internalization of OmpA beads into HL-60 cells (Fig. 16, F and G). KPL-1 , an antibody that is specific for and blocks A.phagocytophilum binding to the PSGL-1 N-terminus, did not affect OmpA bead adherence or uptake (Fig. 16, H and I). These data indicate that OmpA coated beads bind and enter myeloid cells in a sLex- dependent manner and require OmpA residues G61 and 64 to optimally do so.
Delineation of the Aspl4 binding domain
Of the three invasins that cooperatively function to facilitate A. phagocytophilum infection of mammalian host cells, only the binding domain of Asp 14 had yet to be defined. Asp 14 is a 124-amino acid (13.8 kDa) protein, and its binding domain lies within residues 101 to 124. To further narrow down this region, antisera were raised against residues 98 to 1 12 and 1 13 to 124. Both antisera recognized GST-Aspl4, but not GST-Aspl41-88 or GST alone. Also, antiserum targeting Aspl 498-112 but not Aspl41 13-124 detected GST- Aspl 41-112 and each antiserum was specific for the peptide against which it had been raised. Next, the abilities of anti- Aspl498-1 12 and anti-Aspl41 13-124 to inhibit phagocytophilum infection of HL-60 cells were assessed. Incubating DC bacteria with Aspl4113-124 antibody reduced the percentages of infected cells in a dose dependent manner, whereas Asp 1498-1 12 antibody had no effect (Fig. 17A). When used together, antisera against Aspl41 13-124 and OmpA59-74 reduced A.
phagocytophilum by approximately four- fold (Fig. 17B). The observed blocking effect was significantly greater than that achieved with either antiserum alone or when either was paired with antisera that targeted irrelevant regions of OmpA or Asp 14. To ensure that the blocking effects achieved by the OmpA59-74 and Aspl4113-124 antisera were specific, fragment antigen binding (Fab fragment) portions of OmpA23-40, OmpA41-48, OmpA59-74, Aspl498-112, Asp 141 13-124, or OmpA59-74 and Aspl41 13-124 antibodies were prepared and assessed for the ability to inhibit ^, phagocytophilum infection of HL-60 cells. Consistent with results obtained using intact antibodies, OmpA59-74 Fab, Aspl4113-124 Fab, and the combination thereof achieved the greatest reductions in the percentage of infected cells and morulae per cell (Fig. 17, C and D).
An antisera combination targeting the OmpA, Aspl4, and AipA binding domains pronouncedly inhibits A. phagocytophilum infection of host cells
It was shown in Example 1 that a combination of antisera that had been raised against the entireties of OmpA, AipA, and Asp 14 strongly inhibited A. phagocytophilum infection of mammalian host cells. To refine this blocking approach, DC organisms were treated with a cocktail of antibodies specific for OmpA59-74, Aspl41 13-124, and AipA9-21 prior to incubating the bacteria with HL-60 cells. This antibody combination significantly attenuated infection, reducing the percentage of infected cells and number of morulae per cell by approximately five- fold (Fig. 18, A and B). The reduction in infection achieved using the combination antisera was due to effective blocking of bacterial adhesion to HL-60 cell surfaces, as combination antisera specific for OmpA59-74, Aspl4113-124, and AipA9-21 reduced the numbers of bound A. phagocytophilum organisms per cell by more than four-fold relative to the same amount of preimmune serum (Fig. 18C). The observed reductions in bacterial adhesion and infection achieved by targeting all three binding domains were greater than those achieved using (1) antibodies that targeted only one or two of the binding domains and (2) combinations of antibodies against one or two of the binding domains together with antibodies against irrelevant portions of OmpA, Asp 14, or AipA. Thus, targeting the OmpA, Asp 14, and AipA binding domains together produced a synergistic blocking effect that protects host cells from A.
phagocytophilum infection.
DISCUSSION
This study identified the OmpA and Asp 14 binding domains and defined the OmpA residues that are critical for adhesion and invasion. The OmpA binding domain lies within amino acids 59 to 74 and it, like the rest of the protein, is highly conserved among A. phagocytophilum strains known to cause disease in humans and animals. Antibody against OmpA59-74 inhibited bacterial binding to PSGL-1 CHO cells and infection of HL-60 cells. OmpA59-74 is predicted to be a solvent exposed alpha helix and part of a cationic surface patch that binds sLex, an interaction that is similar to those between staphylococcal superantigen-like (SSL) protein family members and sLe\ SSL4, SSL5, and SSL1 1 each use basic residues within cationic surface pockets to interact with a2,3-sialic acid of sLex (Chung et al., 2007; Hermans et al., 2012; Baker et al, 2007). Likewise, other pathogens' sialic acid binding proteins, including uropathogenic Escherichia coli sialic acid-specific S fimbrial adhesin (Morschhauser et al., 1990), pertussis toxin of Bordetella pertussis (Stein et al., 1994), influenza viral neuraminidase (Varghese et al., 1992), canine adenovirus 2 capsid protein (Rademacher et al., 2012), and rhesus rotavirus VP4 (Dormitzer et al, 2002) all use basic residues localized within cationic surface pockets to target sialic acid. The Asp 14 binding domain is within amino acids 1 13 to 124. Antibody specific for Aspl41 13-124 abrogated bacterial binding and infection of host cells. As Aspl4 bears no semblance to any known crystal structure, it could not be modeled. However, from the data presented herein it can be inferred that Asp 14 amino acids 1 13 to 124 are exposed on the surfaces of A. phagocytophilum and the invasin itself.
OmpA K64 is essential for and G61 contributes to the ability of OmpA to bind to mammalian host cells. These experimental findings support the top two OmpA-sLe" docking models, both of which predicted the involvement of K64 and G61 in interacting with a2,3-sialic acid and al ,3-fucose of sLe\ The actual interactions between OmpA and sLex are likely a hybrid of those predicted by the two docking models because, while both predicted the involvement of K64 and G61, one also predicted the involvement of K60, which was found to be negligible for OmpA to act as a competitive agonist. OmpA K64 and G61 may play functionally conserved roles among members of the fdmWyAnaplasmataceae and the genus Anaplasma. K64 is present in all Anaplasma and Ehrlichia spp. OmpA proteins, while G61 is conserved among Anaplasma but not Ehrlichia spp. OmpA proteins. A. marginale agglutinates bovine red blood cells in a sialidase-sensitive manner, indicating that it interacts with sialylated glycans on erythrocyte surfaces. E. chaffeensis OmpA contributes to infection of monocytic cells (Cheng et al., 2011). Compared to the conservation exhibited among Anaplasma spp. OmpA proteins, A.
phagocytophilum and E. chaffeensis OmpA proteins are more divergent in sequence, especially in the binding domain, which may contribute to these pathogens' tropisms for different leukocytes. Still, because of its conservation, the E. chaffeensis OmpA residue that corresponds to A. phagocytophilumOmpA K64 may be involved in binding to a sLex-related glycan on monocytic cells.
Together with a2,3-sialic acid, al,3-fucose is critical for A. phagocytophilum binding and infection. OmpA binds l ,3-fucose, as can be inferred from the observations that recombinant OmpA bound poorly to RF/6A endothelial cells from which l ,3/4-fucose residues had been removed or that had been incubated with the al ,3/6-fucose-specific lectin, AAL. The ability of OmpA to bind a2,3-sialic acid and al,3-fucose is consistent with the close proximity of the two sugar residues to each other in sLex and related glycans and also with OmpA-sLex molecular docking predictions. Yet, RF/6A cells, which support ^, phagocytophilum binding and infection, express very little to no sLex. Rather, they express 6-sulfo-sLex, which presents a2,3 -sialic acid and l ,3-fucose in the same orientation and proximity to each other as sLex. Recombinant OmpA binding to RF/6A cells was significantly reduced in the presence of 6-sulfo-sLex antibody, but not sLex antibodies, thereby supporting that 6-sulfo-sLex is an A. phagocytophilum receptor on these cells. Thus, A. phagocytophilum OmpA interacts with glycans that present a2,3-sialic acid and ccl,3-fucose in a similar manner as sLe\
OmpA by itself functions as both an adhesin and an invasin, as demonstrated by the ability of His-OmpA to confer adhesive and internalization capabilities to inert beads.
Approximately half of the His-OmpA beads that bound to host cells were internalized, a degree of uptake that was similar to that reported for C. burnetii OmpA coated beads (Martinez et ai, 2014). Twenty-fold more OmpA coated beads bound to RF/6A cells than to HL-60 cells.
Similarly, recombinant OmpA binding to RF/6A cells but not to HL-60 cells could be detected by immunofluorescence microscopy and flow cytometry. Nonetheless, the ability of recombinant OmpA to competitively antagonize A. phagocytophilum binding and infection of HL-60 cells demonstrates its ability to bind to the host cells, but it apparently does so at too low an avidity to remain bound during the wash steps associated with sample preparation for the detection methods used. The observed differences in OmpA binding to HL-60 versus RF/6A cells could be due to differences in the levels of sLex and 6-sulfo-sLex on HL-60 and RF/6A cell surfaces or perhaps due to the presence of an additional, undefined OmpA receptor on RF/6A cells. Yet another possibility is that the bacterium binds with a greater avidity to 6-sulfo-sLex than to sLex. Because of the essential and cooperative roles that OmpA, Asp 14, and AipA play in the A. phagocytophilum lifecycle, blocking their ability to function prevents both infection and bacterial survival. Moreover, directing the immune response to their binding domains enhances protective efficacy. In this study, an antibody cocktail specific for the OmpA, Asp 14, and AipA binding domains blocked A. phagocytophilum infection of host cells. The relevance of this work extends to other obligate intracellular pathogens that use multiple invasins, including A.
marginale, E. chaffeensis, spotted fever rickettsiae , Chlamydia spp. , Mycobacterium spp., and Orientia tsutsugamushi, as their survival hinges on their abilities to enter host cells.
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While the invention has been described in terms of its preferred embodiments, those skilled in the art will recognize that the invention can be practiced with modification within the spirit and scope of the appended claims. Accordingly, the present invention should not be limited to the embodiments as described above, but should further include all modifications and equivalents thereof within the spirit and scope of the description provided herein.

Claims

CLAIMS claim:
1. An immunogenic composition comprising a vehicle or carrier suitable for administration to a subject and
a first polypeptide that is or includes one or more copies of SEQ ID NO: 14 and at least one second polypeptide that is or includes one or more copies of SEQ ID NO:03 and SEQ ID NO:06, or
at least one third polypeptide that includes one or more copies of SEQ ID NO: 14 and one or more copies of at least one of SEQ ID NO:03 and SEQ ID NO:06.
2. The immunogenic composition of claim 1 , wherein at least one of said first, second, or third polypeptides is linked to one or more of: an amino acid spacer; an amino acid linker; a signal sequence; a stop transfer sequence; a transmembrane domain; a protein purification ligand; a heterologous protein; or one or more additional polypeptides comprising SEQ ID NO:01 , 02, 03, 04, 05, 06, 07, 08, 09, 13, or 15.
3. The immunogenic composition of claim 2, wherein at least one of said first, second, or third polypeptides is linked to said protein purification ligand and said protein purification ligand is selected from the group consisting of glutathione S-transferase (GST) and a histidine tag (His tag).
4. A chimeric protein comprising a polypeptide that is or includes SEQ ID NO: 14 and one or more polypeptide sequences.
5. The chimeric protein of claim 4, wherein said one or more polypeptide sequences is or includes at least one of SEQ ID NO:03 and SEQ ID NO:06.
6. An isolated nucleic acid comprising a sequence that encodes for at least one polypeptide comprising one or more copies of SEQ ID NO: 14 and at least one of one or more copies of SEQ ID NO:03 and one or more copies of SEQ ID NO:06 on the same or a different polypeptide.
7. A vector comprising the nucleic acid of claim 6.
8. A host cell harboring the nucleic acid of claim 6.
9. A method of protecting or treating a subject from a zoonotic disease comprising the step of administering to said subject an immunogenic composition comprising
a first polypeptide that is or includes one or more copies of SEQ ID NO: 14 and at least one second polypeptide that is or includes one or more copies of SEQ ID NO:03 and SEQ ID NO:06, or
at least one third polypeptide that includes one or more copies of SEQ ID NO: 14 and one or more copies of at least one of SEQ ID NO:03 and SEQ ID NO:06, or
a nucleic acid comprising a sequence that encodes for at least one polypeptide comprising SEQ ID NO: 14 and at least one of SEQ ID NO:03 and SEQ ID NO:06 on the same or a different polypeptide, or
a vector comprising said nucleic acid, or
a host cell harboring said nucleic acid.
10. The method according to claim 9, wherein at least one of said polypeptides or said single polypeptide is linked to an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, or one or more additional polypeptides comprising SEQ ID NO:01, 02, 03, 04, 05, 06, 07, 08, 09, 13, or 15, or a combination thereof.
1 1. The method of claim 9, wherein said zoonotic disease is caused by an obligate
intracellular Anaplasmataceae bacterium selected from the group consisting of
Anaplasma phagocytophilum, Anaplasma marginale, Anaplasma platys, Ehrlichia chaffeensis, Ehrlichia canis, and Ehrlicia ruminatium.
12. The method of claim 9, wherein said subject is a human, and said zoonotic disease is human granulocytic anaplasmosis (HGA).
13. The method of claim 9, wherein said subject is an animal and said zoonotic disease is anaplasmosis.
14. The method of claim 9, wherein said immunogenic composition further comprises at least one peptide selected from the group consisting of SEQ ID NO:01-02, 04-05, 07-12, and 16-68.
15. A method of detecting antibodies that specifically bind an Anaplasmataceae polypeptide in a test sample, comprising the steps of contacting a test sample, under conditions that allow polypeptide-antibody complexes to form, with a composition that includes at least one or more polypeptides encoding all or a portion of SEQ ID NO: 13, and detecting one or more polypeptide-antibody complexes in said test samples, wherein the detection is an indication that antibodies specific for Anaplasmataceae AipA are present in the test sample.
16. The method of claim 15, wherein said method is an assay selected from the group
consisting of an immunoblot and an enzyme-linked immunosorbent assay (ELISA).
17. The method of claim 15, wherein said polypeptide encodes SEQ ID NO: 14.
PCT/US2015/013715 2014-02-03 2015-01-30 Aipa, ompa, and asp14 in vaccine compositions and diagnostic targets for anaplasma phagocytophilum infection WO2015116907A1 (en)

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