WO2015038796A3 - Secretion of heme-containing polypeptides - Google Patents

Secretion of heme-containing polypeptides Download PDF

Info

Publication number
WO2015038796A3
WO2015038796A3 PCT/US2014/055227 US2014055227W WO2015038796A3 WO 2015038796 A3 WO2015038796 A3 WO 2015038796A3 US 2014055227 W US2014055227 W US 2014055227W WO 2015038796 A3 WO2015038796 A3 WO 2015038796A3
Authority
WO
WIPO (PCT)
Prior art keywords
heme
secretion
containing polypeptides
polypeptides
expression
Prior art date
Application number
PCT/US2014/055227
Other languages
French (fr)
Other versions
WO2015038796A2 (en
Inventor
Rachel FRASER
Simon Christopher Davis
Patrick O'Rielly BROWN
Original Assignee
Impossible Foods Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN201480057829.3A priority Critical patent/CN105745332A/en
Application filed by Impossible Foods Inc. filed Critical Impossible Foods Inc.
Priority to EP20157598.2A priority patent/EP3722431A1/en
Priority to SI201431544T priority patent/SI3044320T1/en
Priority to US15/021,447 priority patent/US20160340411A1/en
Priority to PL14844701T priority patent/PL3044320T3/en
Priority to ES14844701T priority patent/ES2791364T3/en
Priority to RS20200343A priority patent/RS60259B1/en
Priority to LTEP14844701.4T priority patent/LT3044320T/en
Priority to EP14844701.4A priority patent/EP3044320B1/en
Priority to DK14844701.4T priority patent/DK3044320T3/en
Publication of WO2015038796A2 publication Critical patent/WO2015038796A2/en
Publication of WO2015038796A3 publication Critical patent/WO2015038796A3/en
Priority to US15/672,466 priority patent/US20170342131A1/en
Priority to US15/672,579 priority patent/US20170342132A1/en
Priority to HRP20200447TT priority patent/HRP20200447T1/en
Priority to CY20201100436T priority patent/CY1122898T1/en
Priority to US17/081,798 priority patent/US20210070842A1/en
Priority to US18/307,144 priority patent/US20230365657A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/22—Working-up of proteins for foodstuffs by texturising
    • A23J3/225—Texturised simulated foods with high protein content
    • A23J3/227—Meat-like textured foods
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42—Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/424—Addition of non-meat animal protein material, e.g. blood, egg, dairy products, fish; Proteins from microorganisms, yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y499/00—Other lyases (4.99)
    • C12Y499/01—Other lyases (4.99.1)
    • C12Y499/01001—Ferrochelatase (4.99.1.1)

Abstract

This disclosure provides for methods and compositions for the expression and secretion of heme-containing polypeptides.
PCT/US2014/055227 2013-01-11 2014-09-11 Secretion of heme-containing polypeptides WO2015038796A2 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
LTEP14844701.4T LT3044320T (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
RS20200343A RS60259B1 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
EP20157598.2A EP3722431A1 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
SI201431544T SI3044320T1 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
US15/021,447 US20160340411A1 (en) 2013-01-11 2014-09-11 Secretion of heme-containing polypeptides
PL14844701T PL3044320T3 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
ES14844701T ES2791364T3 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
CN201480057829.3A CN105745332A (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
EP14844701.4A EP3044320B1 (en) 2013-09-11 2014-09-11 Secretion of heme-containing polypeptides
DK14844701.4T DK3044320T3 (en) 2013-09-11 2014-09-11 SECRETARY OF SECRET POLYPEPTIDES
US15/672,579 US20170342132A1 (en) 2013-09-11 2017-08-09 Secretion of heme-containing polypeptides
US15/672,466 US20170342131A1 (en) 2013-09-11 2017-08-09 Secretion of heme-containing polypeptides
HRP20200447TT HRP20200447T1 (en) 2013-09-11 2020-03-18 Secretion of heme-containing polypeptides
CY20201100436T CY1122898T1 (en) 2013-09-11 2020-05-12 EXCRETION OF HEME-CONTAINING POLYPEPTIDES
US17/081,798 US20210070842A1 (en) 2013-09-11 2020-10-27 Secretion of heme-containing polypeptides
US18/307,144 US20230365657A1 (en) 2013-09-11 2023-04-26 Secretion of heme-containing polypeptides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361876676P 2013-09-11 2013-09-11
US61/876,676 2013-09-11
US201361908689P 2013-11-25 2013-11-25
US61/908,689 2013-11-25

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US15/021,447 A-371-Of-International US20160340411A1 (en) 2013-01-11 2014-09-11 Secretion of heme-containing polypeptides
US15/672,579 Continuation US20170342132A1 (en) 2013-09-11 2017-08-09 Secretion of heme-containing polypeptides
US15/672,466 Continuation US20170342131A1 (en) 2013-09-11 2017-08-09 Secretion of heme-containing polypeptides

Publications (2)

Publication Number Publication Date
WO2015038796A2 WO2015038796A2 (en) 2015-03-19
WO2015038796A3 true WO2015038796A3 (en) 2015-10-29

Family

ID=52666512

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/055227 WO2015038796A2 (en) 2013-01-11 2014-09-11 Secretion of heme-containing polypeptides

Country Status (14)

Country Link
US (4) US20170342131A1 (en)
EP (2) EP3044320B1 (en)
CN (1) CN105745332A (en)
CY (1) CY1122898T1 (en)
DK (1) DK3044320T3 (en)
ES (1) ES2791364T3 (en)
HR (1) HRP20200447T1 (en)
HU (1) HUE049764T2 (en)
LT (1) LT3044320T (en)
PL (1) PL3044320T3 (en)
PT (1) PT3044320T (en)
RS (1) RS60259B1 (en)
SI (1) SI3044320T1 (en)
WO (1) WO2015038796A2 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230031993A (en) 2015-05-11 2023-03-07 임파서블 푸즈 인크. Expression constructs and methods of genetically engineering methylotrophic yeast
WO2017100376A2 (en) 2015-12-07 2017-06-15 Zymergen, Inc. Promoters from corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
JP2019519242A (en) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド Method for generating a bacterial hemoglobin library and its use
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. Methods for generating a glucose permease library and uses thereof
EP3535416A4 (en) * 2016-11-04 2020-05-13 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
CN108866132B (en) * 2018-06-20 2021-09-17 浙江海洋大学 Process for extracting squid active small peptide at ultralow temperature
CN111286520B (en) * 2018-12-10 2021-05-07 上海凯赛生物技术股份有限公司 Recombinant DNA for fermentation production of L-lysine, strain and application thereof
GB2603289A (en) * 2019-05-21 2022-08-03 Univ Kent State Ohio Animal cell lines for foods containing cultured animal cells
IL294248A (en) * 2019-12-31 2022-08-01 Air Protein Inc High protein food compositions
CN111138513B (en) * 2020-01-06 2022-10-18 天津科技大学 Screening of Cross-Linked peptides of Glutamine transaminase
US20230073947A1 (en) * 2020-01-10 2023-03-09 Intron Biotechnology, Inc. A method for preparing bovine myoglobin using escherichia coli
EP4090678A4 (en) * 2020-01-10 2024-01-10 Intron Biotechnology Inc A method for preparing porcine myoglobin using escherichia coli
US20230074134A1 (en) * 2020-01-10 2023-03-09 Intron Biotechnology, Inc. Method for preparing soy leghemoglobin using escherichia coli
CA3171066A1 (en) * 2020-03-11 2021-09-16 Saar GILL Methods and composition for gene delivery using an engineered viral particle
US20230101863A1 (en) * 2020-03-27 2023-03-30 Air Protein, Inc Structured high-protein meat analogue compositions with microbial heme flavorants
CN111549050B (en) * 2020-05-18 2021-09-17 湖北大学 Vitreoscilla hemoglobin expression frame suitable for bacillus and application
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
IL301396A (en) 2020-09-30 2023-05-01 Nobell Foods Inc Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
WO2022072846A2 (en) 2020-10-02 2022-04-07 Impossible Foods Inc. Transgenic plants with altered fatty acid profiles and upregulated heme biosynthesis
WO2022072833A2 (en) 2020-10-02 2022-04-07 Impossible Foods Inc. Expression constructs and methods of genetically engineering cells
CN116507728A (en) 2020-10-28 2023-07-28 先锋国际良种公司 Leghemoglobin in soybeans
AU2021391607A1 (en) * 2020-12-02 2023-06-29 Planeat Foods Pte. Ltd. Method of producing globin polypeptide recombinantly and meat substitute food product
CA3202911A1 (en) * 2020-12-31 2022-07-07 Hermes SANCTORUM Meat substitute comprising animal myoglobin
CN112680434B (en) * 2021-02-01 2022-12-02 武汉新华扬生物股份有限公司 Method for improving secretory expression of protein glutaminase
CN113150120B (en) * 2021-05-21 2022-09-30 江南大学 Separation and purification method of porcine myoglobin in fermentation liquor
US11639515B2 (en) 2021-05-21 2023-05-02 Taixing Dongsheng Bio-Tech Co., Ltd Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof
WO2022242033A1 (en) * 2021-05-21 2022-11-24 江苏东汇生物科技有限公司 Genetically engineered bacterium for producing porcine myoglobin, and fermentation and purification of same
WO2022251166A2 (en) * 2021-05-25 2022-12-01 Evelo Biosciences, Inc. Bacterial compositions comprising soy hemoglobin
CN113755352B (en) * 2021-08-13 2023-04-14 中国科学院天津工业生物技术研究所 Construction and application of saccharomyces cerevisiae genetic engineering bacteria for producing heme by fermentation method
WO2023039012A1 (en) * 2021-09-07 2023-03-16 The Regents Of The University Of California Expression of heme biosynthesis and heme proteins in edible filamentous fungi
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
WO2023137192A1 (en) * 2022-01-15 2023-07-20 The Fynder Group, Inc. Meat replica fungal food product
WO2024003668A1 (en) * 2022-06-29 2024-01-04 Moolec Science Limited High expression of animal heme protein in plants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080166757A1 (en) * 2000-09-18 2008-07-10 Sierd Bron Twin-arginine translocation in Bacillus
WO2012083424A1 (en) * 2010-12-21 2012-06-28 The University Of Western Ontario Novel amino acid linker sequences for ligand immobilization

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5753465A (en) * 1994-08-30 1998-05-19 Carnegie Mellon University Unmodified recombinant human adult hemoglobin production
FR2736930B1 (en) * 1995-07-17 1997-09-19 Biocem PROCESS FOR THE PRODUCTION, BY PLANT CELLS, OF HEMINIC PROTEINS, PROTEINS THUS OBTAINED AND PRODUCTS CONTAINING THE SAME
US5959187A (en) 1996-09-26 1999-09-28 Bailey; James E. Expression of oxygen-binding proteins in plants
JPH10117776A (en) 1996-10-22 1998-05-12 Japan Tobacco Inc Transformation of indica rice
WO2000000597A2 (en) * 1998-06-26 2000-01-06 The University Of Manitoba Non-symbiotic plant hemoglobins to maintain cell energy status
WO2004057946A2 (en) * 2002-12-23 2004-07-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaft e.V. Method for altering the content of reserve substances in plants
AU2003218396B2 (en) * 2003-04-11 2008-09-04 Ventria Bioscience Human blood proteins expressed in monocot seeds
US20080193969A1 (en) * 2005-01-12 2008-08-14 Rob Te Biesebeke Hemoglobin Overexpression in Fungal Fermentations
US20100064384A1 (en) * 2006-04-13 2010-03-11 Max Planck Institut fur molekulare Method for modifying the atp/adp ratio in cells
CN103889243A (en) 2011-07-12 2014-06-25 马拉克西公司 Methods and compositions for consumables
EP4104682A3 (en) 2013-01-11 2023-02-22 Impossible Foods Inc. Methods and compositions for affecting the flavour and aroma profile of consumables

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080166757A1 (en) * 2000-09-18 2008-07-10 Sierd Bron Twin-arginine translocation in Bacillus
WO2012083424A1 (en) * 2010-12-21 2012-06-28 The University Of Western Ontario Novel amino acid linker sequences for ligand immobilization

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LONDER ET AL.: "Production and preliminary characterization of a recombinant triheme cytochrome c(7) from Geobacter sulfurreducens in Escherichia coli.", BIOCHEM BIOPHYS ACTA, vol. 1554, no. 3, 1 July 2002 (2002-07-01), pages 202 - 211, XP 004373902, ISSN: 0005-2728 *
WU ET AL.: "Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system.", BIO/TECHNOLOGY ( NY, vol. 11, no. 1, January 1993 (1993-01-01), pages 71 - 76, XP055329301 *

Also Published As

Publication number Publication date
CY1122898T1 (en) 2021-05-05
LT3044320T (en) 2020-06-10
US20230365657A1 (en) 2023-11-16
ES2791364T3 (en) 2020-11-04
PL3044320T3 (en) 2020-07-13
DK3044320T3 (en) 2020-03-16
US20170342131A1 (en) 2017-11-30
CN105745332A (en) 2016-07-06
EP3044320A4 (en) 2017-03-08
EP3044320A2 (en) 2016-07-20
HUE049764T2 (en) 2020-10-28
PT3044320T (en) 2020-04-13
SI3044320T1 (en) 2020-07-31
EP3044320B1 (en) 2020-02-19
HRP20200447T1 (en) 2020-06-12
RS60259B1 (en) 2020-06-30
EP3722431A1 (en) 2020-10-14
WO2015038796A2 (en) 2015-03-19
US20210070842A1 (en) 2021-03-11
US20170342132A1 (en) 2017-11-30

Similar Documents

Publication Publication Date Title
WO2015038796A3 (en) Secretion of heme-containing polypeptides
EP3347472A4 (en) Compositions comprising cyp76ad1- clade polypeptides and uses thereof
EP3364951A4 (en) Nicotine composition for vaping devices and vaping devices employing the same
EP3119424A4 (en) Immunogenic glycopeptides, composition comprising the glycopeptides and use thereof
EP3353213A4 (en) Novel anti-mesothelin antibody and composition comprising the same
WO2015051030A8 (en) Stabilized polypeptides and uses thereof
WO2014152157A8 (en) Methods and compositions for the generation and use of conformation-specific antibodies
EP3015526A4 (en) Halo-olefin composition and use therefor
EP3182985A4 (en) Fibroin-derived protein composition
EP3179983A4 (en) Anti-methanogenic compositions and uses thereof
EP3436083A4 (en) Novel compositions and methods
EP3166892A4 (en) Scale-inhibition compositions and methods of making and using the same
EP3305802A4 (en) Novel peptide and composition containing the same
EP3117827A4 (en) Composition for vagina and use of the composition
WO2016079110A3 (en) Use of enzyme for cleaning
EP3094379A4 (en) Enolase 1 (eno1) compositions and uses thereof
SG10201900598TA (en) Factor viii formulation
EP3309167A4 (en) Small-molecule polypeptide zy4 and application thereof
WO2016019036A3 (en) Recombinant collagen iv surrogates and uses thereof
EP3316871A4 (en) Talc-bound compositions and uses thereof
EP3153492A4 (en) Novel compound and fragrance composition containing same
EP3134465A4 (en) Improved coating compositions and processes for making the same
EP3096742A4 (en) Compositions and methods for modifying the surface of cells and methods of use
EP3247362A4 (en) Onapristone extended-release compositions and methods
EP3113774A4 (en) Compositions of grapiprant and methods for using the same

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 15021447

Country of ref document: US

REEP Request for entry into the european phase

Ref document number: 2014844701

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2014844701

Country of ref document: EP

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14844701

Country of ref document: EP

Kind code of ref document: A2