WO2015031477A1 - Enzyme composition and uses thereof - Google Patents
Enzyme composition and uses thereof Download PDFInfo
- Publication number
- WO2015031477A1 WO2015031477A1 PCT/US2014/052905 US2014052905W WO2015031477A1 WO 2015031477 A1 WO2015031477 A1 WO 2015031477A1 US 2014052905 W US2014052905 W US 2014052905W WO 2015031477 A1 WO2015031477 A1 WO 2015031477A1
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- WO
- WIPO (PCT)
- Prior art keywords
- seq
- glucoamylase
- amylase
- enzyme composition
- alpha
- Prior art date
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention relates to enzyme compositions and processes of producing sugars and/or fermentation products from starch-containing materials using such enzyme compositions.
- starch When producing fermentation products, such as ethanol, starch is conventionally converted into dextrins using a liquefying enzyme (e.g., Bacillus alpha-amylase) at temperatures above the initial gelatinization temperature of starch to above 100°C.
- the generated dextrins are hydrolyzed into sugars using a saccharifying enzyme (e.g., glucoamylase) and fermented into the desired fermentation product using a fermenting organism such as a yeast strain derived from Saccharomyces cerevisiae.
- a saccharifying enzyme e.g., glucoamylase
- a fermenting organism such as a yeast strain derived from Saccharomyces cerevisiae.
- SSF simultaneous saccharification and fermentation
- US4514496 concerns a process for producing alcohol by mixing a ground starchy material with mashing liquor without cooking, adding a saccharifying enzyme preparation derived from a Rhizopus sp., and adding an alcoholic fermenting yeast, and fermenting the slurry.
- WO2003/066826 discloses a method for producing an alcohol by contacting a carbon substrate and at least one substrate-converting enzyme to produce an intermediate, and contacting said intermediate with at least one intermediate-converting enzyme.
- WO 2004/080923 concerns a process for production of an alcohol product comprising the sequential steps of providing a slurry comprising water and granular starch; holding said slurry in the presence of an acid alpha-amylase and a glucoamylase at a temperature of 0°C to 20°C below the initial gelatinization temperature of said granular starch for a period of 5 minutes to 12 hours; holding said slurry in the presence of an acid alpha-amylase and a glucoamylase and a yeast at a temperature between 10°C and 35°C for a period of 20 to 250 hours to produce ethanol.
- a xylanase, cellulase and phytase may be present during the holding steps.
- WO2004/081 193 concerns a process for producing ethanol from plant material, comprising reducing the plant material to produce material comprising starch, saccharifying the starch, without cooking, with an enzyme composition, and fermenting the incubated starch to yield a composition comprising at least 15 vol-% ethanol.
- WO2006/069289 concerns glucoamylases derived from Trametes cingulata, Pachykytospora papyracea, and Leucopaxillus giganteus and the use thereof in a process for producing a fermentation product from starch-containing material at a temperature below the initial gelatinization temperature.
- WO 201 1/068803 discloses glucoamylase derived from Gloeophyllum sepiarium, Gloeophyllum trabeum, or Gloeophyllum abietinum and the use thereof in processes of producing a fermentation product from starch-containing material.
- the present invention is concerns enzyme compositions suitable for raw starch hydrolysis processes and the use of such enzyme compositions in such processes.
- the invention relates to enzyme composition comprising a number of enzyme activities and the use thereof in processes of producing sugars and/or fermentation products, such as especially ethanol.
- the enzyme composition of the invention may be a blend of a number of difference enzyme activities.
- the enzyme activities may be of the same or difference origin.
- the present invention relates enzyme compositions comprising glucoamylase and alpha-amylase, and optionally protease.
- the enzyme composition comprises Gloeophyllum glucoamylase, preferably Gloeophyllum trabeum glucoamylase and an alpha-amylase.
- the alpha-amylase is derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein.
- SBD starch-binding domain
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P; and an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch- binding domain (SBD), preferably the one shown in SEQ I D NO: 13 herein, preferably having one or more of the following substitutions: G128D, D143N, especially G128D+D143N.
- SBD alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch- binding domain
- the enzyme composition comprises a glucoamylase derived from a strain of the genus Pycnoporus, in particular a strain of Pycnoporus sanguineus, such as a strain described in WO 201 1/066576 (SEQ ID NOs 2, 4 or 6).
- the enzyme composition comprises the glucoamylase shown in SEQ ID NO: 4 in WO 201 1/066576 or SEQ ID NO: 17 herein and an alpha-amylase.
- the alpha-amylase is derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein.
- SBD starch-binding domain
- the enzyme composition comprises the Pycnoporus sanguineus glucoamylase shown in SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ I D NO: 17 herein, and an alpha- amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18, preferably having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P; and an alpha- amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%,
- the enzyme composition comprises the Pycnoporus sanguineus glucoamylase shown in SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 17, and an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch- binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, or an alpha- amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%
- SBD
- a protease is comprised in the enzyme composition of the invention.
- the protease is a metallo protease or a serine protease.
- the enzyme composition comprises a metallo protease, preferably derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- a metallo protease preferably derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- the protease is derived from a strain of Pyrococcus, such as a strain of Pyrococcus furiosus, such as the protease shown in SEQ ID NO: 1 in US 6,358,726 or SEQ ID NO: 5 herein.
- the protease is the mature sequence from Meripilus giganteus protease 3 (peptidase family S53 protease) concerned in Example 2 in WO 2014/037438 and shown as SEQ ID NO: 20 herein.
- the protease is the mature protease 3 sequence from Meripilus giganteus shown as SEQ ID NO: 19 herein and SEQ ID NO: 5 in WO 2014/037438.
- the ratio between glucoamylase and alpha-amylase is between 99:1 and 1 :2, such as between 98:2 and 1 :1 , such as between 97:3 and 2:1 , such as between 96:4 and 3:1 , such as 97:3, 96:4, 95:5, 94:6, 93:7, 90:10, 85:15 (mg EP(Enzyme Protein) glucoamylase: mg EP (Enzyme Protein) alpha-amylase).
- the invention relates to processes of producing fermentation products, such as especially ethanol, from starch-containing material, such as granular starch, comprising:
- saccharification and/or fermentation is done in the presence of the following enzymes: glucoamylase and alpha-amylase, and optionally protease.
- the following enzymes are present and/or added during saccharification and/or fermentation: the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18, preferably one having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P, and an alpha-amylase.
- the alpha-amylase is derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein.
- SBD starch-binding domain
- the following enzymes are present and/or added during saccharification and/or fermentation: the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18 herein, preferably one having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P, and alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least
- the following enzymes are present and/or added during saccharification and/or fermentation: the Pycnoporus sanguineus glucoamylase shown in SEQ ID NO: 17 herein and an alpha-amylase.
- the alpha-amylase is derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein, preferably one having one or more of the following substitutions: G128D, D143N, especially G128D+D143N.
- SBD starch-binding domain
- a protease is present and/or added during saccharification and/or fermentation.
- the protease is a metallo protease or a serine protease.
- the metallo protease is derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- the protease is a peptidase family S53 protease derived from a strain of Meripilus, preferably a strain of Meripilus giganteus.
- the protease is the mature sequence from Meripilus giganteus protease 3 (peptidase family S53 protease) concerned in Example 2 in WO 2014/037438 and shown as SEQ ID NO: 20 herein.
- the protease is the mature protease 3 sequence from Meripilus giganteus shown as SEQ ID NO: 19 herein and SEQ ID NO: 5 in WO 2014/037438.
- the invention relates to processes of producing fermentation products, such as especially ethanol, from starch-containing material, such as granular starch, comprising:
- the following enzymes are present and/or added during saccharification and/or fermentation: the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, preferably one having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P, and alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, preferably one having one or more of the following substitutions: G128D, D143N, especially G128D+143N, and a cellulolytic enzyme composition derived from Trichoderma reesei, preferably further comprising Thermoascus aurantiacus GH61A polypeptide having cellulolytic enhancing activity (e.g., SEQ I D NO: 2 in WO 2005/074656 or SEQ ID
- a protease is present and/or added during saccharification and/or fermentation.
- the protease is a metallo protease or a serine protease.
- the metallo protease is derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- the protease is a peptidase family S53 protease derived from a strain of Meripilus, preferably a strain of Meripilus giganteus.
- the protease is the mature sequence from Meripilus giganteus protease 3 (peptidase family S53 protease) concerned in Example 2 in WO 2014/037438 and shown as SEQ ID NO: 20 herein, or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 20.
- the protease is the mature protease 3 sequence from Meripilus giganteus shown as SEQ ID NO: 19 herein and SEQ ID NO: 5 in
- ratio between glucoamylase and alpha-amylase is between 99:1 and 1 :2, such as between 98:2 and 1 :1 , such as between 97:3 and 2:1 , such as between 96:4 and 3:1 , such as 97:3, 96:4, 95:5, 94:6, 93:7, 90:10, 85:15, 83:17 or 65:35 (mg EP glucoamylase: mg EP alpha-amylase).
- the total dose of glucoamylase and alpha-amylase is from 10-1 ,000 g/g DS, such as from 50-500 ⁇ g/g DS, such as 75-250 ⁇ g/g DS.
- the total dose of cellulolytic enzyme composition added is from 10-500 ⁇ g/g DS, such as from 20-400 ⁇ g/g DS, such as 20-300 ⁇ g/g DS.
- the dose of protease added is from 1-200 ⁇ g/g DS, such as from 2-
- saccharification step (a) and fermentation step (b) are carried out simultaneously.
- an enzyme composition described above is used in a process of the invention.
- the invention relates to enzyme composition comprising a number of enzyme activities and the use thereof in processes of producing sugars and/or fermentation products, such as especially ethanol.
- An enzyme composition of the invention is suitable for use in 5 processes for producing sugars and/or fermentation products carried out as a raw starch hydrolysis process (i.e., no cook process) at temperatures below the initial gelatinization temperature of the starch in question.
- a raw starch hydrolysis process i.e., no cook process
- the yield of the fermentation product is improved compared to a corresponding process where the glucoamylase or alpha-amylase is used alone or where an enzyme composition consisting of glucoamylase from Trametes cingulata and alpha-amylase from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain
- the present invention relates to enzyme compositions comprising glucoamylase and alpha-amylase, and optionally protease.
- the enzyme composition comprises a Gloeophyllum glucoamylase, preferably Gloeophyllum trabeum glucoamylase, especially the Gloeophyllum 20 trabeum glucoamylase shown in SEQ ID NO: 18, and an alpha-amylase.
- the glucoamylase is derived from Gloeophyllum trabeum, such as the one shown in SEQ ID NO: 18 herein, or a glucoamylase selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 18 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 25 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- ID NO: 18 herein has one of the following substitutions: V59A; S95P; A121 P; T1 19W; 30 S95P+A121 P; V59A+S95P; S95P+T1 19W; V59A+S95P+A121 P; or S95P+T1 19W+A121 P, especially S95P+A121 P.
- the alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein, or an 35 alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 13 herein.
- SBD Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain
- the alpha-amylase is the Rhizomucor pusillus alpha-amylase or the Rhizomucor pusillus alpha-amylase with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), especially one having at least one of the following substitutions or combinations of substitutions: D165M; Y141 W; Y141 R; K136F; K192R; P224A; P224R;
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase, preferably the one shown in SEQ ID NO: 18 and an alpha-amylase derived from Rhizomucor pusillus, preferably with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 18 and an alpha-amylase derived from Rhizomucor pusillus, preferably with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID
- SBD starch-binding domain
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase, preferably the one shown in SEQ ID NO: 18 herein, especially one having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P (using SEQ ID NO: 13 herein for numbering); and the alpha-amylase derived from Rhizomucor pusillus preferably with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably one shown in SEQ ID NO: 13 herein, preferably one having one or more of the following substitutions: G128D, D143N, especially especially G128D+D143N (using SEQ ID NO: 13 herein for numbering).
- SBD Aspergillus niger glucoamylase linker and starch-binding domain
- the enzyme composition comprises a glucoamylase derived from a strain of the genus Pycnoporus, in particular a strain of Pycnoporus sanguineus, such as a strain described in WO 201 1/066576 (SEQ ID NOs 2, 4 or 6).
- the enzyme composition comprises the glucoamylase shown in SEQ ID NO: 4 in WO 201 1/066576 or SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 17 herein, and an alpha-amylase.
- the alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 13 herein.
- SBD Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain
- the enzyme composition comprises the Pycnoporus sanguineus glucoamylase shown in SEQ ID NO: 17 herein; or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 17 herein, and the alpha- amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%,
- SBD
- protease optionally iv) protease.
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, preferably having one or more of the following substitutions: S95P, A121 P, especially S95P+A121 P; and the alpha- amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, preferably having one or more of the following substitutions: G128D, D143N, especially G128D+D143N, and a cellulolytic enzyme composition derived from Trichoderma reesei , preferably further comprising Thermoascus aurantiacus GH61A polypeptide having cellulolytic enhancing activity (SEQ ID NO: 2 in WO 2005/074656 or SEQ ID NO: 9 herein) and Aspergillus fumigatus beta-glu
- the enzyme composition comprises a metallo protease, preferably derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo
- the protease is a peptidase family S53 protease derived from a strain of Meripilus, preferably a strain of Meripilus giganteus.
- the protease is the mature sequence from Meripilus giganteus protease 3 (peptidase family S53 protease) concerned in Example 2 in WO 2014/037438 and shown as SEQ ID NO: 20 herein or a proteaase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least
- protease is the mature protease 3 sequence from Meripilus giganteus shown as SEQ ID NO: 19 herein and SEQ ID NO: 5 in WO 2014/037438 or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least
- enzymes may also be present.
- the specifically contemplated enzyme components are described further below.
- An enzyme composition of the invention optionally contains a cellulolytic enzyme composition.
- the cellulolytic enzyme composition consists of or comprises one or more cellulolytic enzymes.
- the cellulolytic enzyme composition may be of any origin.
- the cellulolytic enzyme composition comprises cellulolytic enzymes of fungal
- the cellulolytic enzyme composition is derived from a strain of Trichoderma, such as Trichoderma reesei; or a strain of Humicola, such as Humicola insolens; or a strain of Chrysosporium, such as Chrysosporium lucknowense; or a strain of Penicillium, such as Penicillium decumbens.
- the cellulolytic enzyme composition is derived from a strain of Trichoderma reesei.
- the cellulolytic enzyme composition may comprise a beta-glucosidase, a cellobiohydrolase, and an endoglucanase.
- the cellulolytic enzyme composition further comprises a GH61 polypeptide having cellulolytic enhancing activity.
- Cellulolytic enhancing activity is defined and determined as described in WO 201 1/041397 (incorporated by reference).
- GH61 polypeptide having cellulolytic enhancing activity means a GH61 polypeptide that enhances the hydrolysis of a cellulosic material by enzymes having cellulolytic activity.
- cellulolytic enhancing activity is determined by measuring the increase in reducing sugars or the increase of the total of cellobiose and glucose from hydrolysis of a cellulosic material by cellulolytic enzyme under the following conditions: 1-50 mg of total protein/g of cellulose in PCS (Pretreated Corn Stover), wherein total protein is comprised of 50-99.5% w/w cellulolytic enzyme protein and 0.5-50% w/w protein of a GH61 polypeptide having cellulolytic enhancing activity for 1-7 days at 50°C compared to a control hydrolysis with equal total protein loading without cellulolytic enhancing activity (1 -50 mg of cellulolytic protein/g of cellulose in PCS).
- a mixture of CELLUCLASTTM1 .5L (Novozymes A/S, Bagsvaerd, Denmark) in the presence of 2-3% of total protein weight Aspergillus oryzae beta-glucosidase (recombinantly produced in Aspergillus oryzae according to WO 02/095014) or 2-3% of total protein weight Aspergillus fumigatus beta-glucosidase (recombinantly produced in Aspergillus oryzae as described in WO 2002/095014) of cellulase protein loading is used as the source of the cellulolytic activity.
- the cellulolytic enzyme composition comprises a beta-glucosidase, preferably one derived from a strain of the genus Aspergillus, such as Aspergillus oryzae, such as the one disclosed in WO 2002/095014 or the fusion protein having beta-glucosidase activity disclosed in WO 2008/057637 (see SEQ ID NOs: 74 or 76), or Aspergillus fumigatus, such as one disclosed in SEQ ID NO: 2 in WO 2005/047499 or SEQ ID NO: 8 herein; or an Aspergillus fumigatus beta-glucosidase variant disclosed in WO 2012/044915; or a strain of the genus a strain Penicillium, such as a strain of the Penicillium brasilianum disclosed in WO 2007/019442, or a strain of the genus Trichoderma, such as a strain of Trichoderma reesei.
- the beta-glucosidase is from a strain of Aspergillus, such as a strain of Aspergillus fumigatus, such as Aspergillus fumigatus beta-glucosidase (SEQ ID NO: 8 herein), or a variant thereof, which variant comprises one or more substitutions selected from the group consisting of L89M, G91 L, F100D, 1140V, 1186V, S283G, N456E, and F512Y; such as a variant thereof with the following substitutions:
- the parent beta-glucosidase has at least 60% identity, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99% identity to the mature polypeptide of SEQ ID NO: 8 herein.
- beta-glucosidase is a beta-glucosidase variant it has at least 60% identity, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, but less than 100% identity to the mature polypeptide of SEQ ID NO: 8 herein.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide
- it may be one derived from the genus Thermoascus, such as a strain of Thermoascus aurantiacus, such as the one described in WO 2005/074656 as SEQ ID NO: 2 or SEQ ID NO: 9 herein; or one derived from the genus Thielavia, such as a strain of Thielavia terrestris, such as the one described in WO 2005/074647 as SEQ ID NO: 7 and SEQ ID NO: 8; or one derived from a strain of Aspergillus, such as a strain of Aspergillus fumigatus, such as the one described in WO 2010/138754 as SEQ ID NO: 1 and SEQ ID NO: 2; or one derived from a strain derived from Penicillium, such as a strain of Penicillium emersonii, such as the one disclosed
- the GH61 polypeptide such as one derived from a strain of Penicillium sp., is selected from the group consisting of:
- a GH61 polypeptide comprising the mature polypeptide of SEQ ID NO: 10 herein;
- a GH61 polypeptide comprising an amino acid sequence having at least 60%, such as at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 10 herein.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a cellobiohydrolase I (CBH I), such as one derived from a strain of the genus Aspergillus, such as a strain of Aspergillus fumigatus, such as the Cel7a CBHI disclosed in SEQ ID NO: 6 in WO 201 1/057140 or SEQ ID NO: 6 herein, or a strain of the genus Trichoderma, such as a strain of Trichoderma reesei.
- CBH I cellobiohydrolase I
- the cellobiohydrolase I such as one derived from a strain of Aspergillus fumigatus, is selected from the group consisting of:
- a cellobiohydrolase I comprising an amino acid sequence having at least 60%, such as at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 6 herein.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a cellobiohydrolase II (CBH II), such as one derived from a strain of the genus Aspergillus, such as a strain of Aspergillus fumigatus; such as the one disclosed as SEQ ID NO: 7 herein or a strain of the genus Trichoderma, such as Trichoderma reesei, or a strain of the genus Thielavia, such as a strain of Thielavia terrestris, such as cellobiohydrolase II CEL6A from Thielavia terrestris.
- CBH II cellobiohydrolase II
- cellobiohydrolase II such as one derived from a strain of Aspergillus fumigatus, is selected from the group consisting of:
- a cellobiohydrolase II comprising the mature polypeptide of SEQ ID NO: 7 herein;
- a cellobiohydrolase II comprising an amino acid sequence having at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide having cellulolytic enhancing activity and a beta-glucosidase.
- the cellulolytic enzyme composition comprises a GH61 polypeptide having cellulolytic enhancing activity derived from a strain of Penicillium, such as a strain of
- Penicillium emersonii such as the one disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, and a beta-glucosidase.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide having cellulolytic enhancing activity, a beta-glucosidase, and a CBHI.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide having cellulolytic enhancing activity derived from a strain of Penicillium, such as a strain of Penicillium emersonii, such as the one disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, a beta- glucosidase, and a CBHI.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide having cellulolytic enhancing activity, a beta-glucosidase, a CBHI, and a CBHII.
- the cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises a GH61 polypeptide having cellulolytic enhancing activity derived from a strain of Penicillium, such as a strain of Penicillium emersonii, such as the one disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, a beta- glucosidase, a CBHI, and a CBHII.
- the cellulolytic enzyme composition is a Trichoderma reesei cellulolytic composition, further comprising Thermoascus aurantiacus GH61A polypeptide (SEQ ID NO: 2 in WO 2005/074656 or SEQ ID NO: 9 herein), and Aspergillus oryzae beta- glucosidase fusion protein (WO 2008/057637).
- the cellulolytic enzyme composition is a Trichoderma reesei cellulolytic composition, further comprising Thermoascus aurantiacus GH61A polypeptide having cellulolytic enhancing activity (SEQ ID NO: 2 in WO 2005/074656 or SEQ ID NO: 9 herein) and Aspergillus fumigatus beta-glucosidase (SEQ ID NO: 2 of WO 2005/047499 or SEQ ID NO: 8 herein).
- the cellulolytic enzyme composition is a Trichoderma reesei cellulolytic composition further comprising Penicillium emersonii GH61A polypeptide disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, and Aspergillus fumigatus beta-glucosidase disclosed as SEQ ID NO: 2 in WO 2005/047499 or SEQ ID NO: 8 herein, or a variant thereof, which variant has one of, preferably all of, the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH1 , e.g., the one disclosed as SEQ ID NO: 6 in WO201 1/057140 and SEQ ID NO: 6 herein and Aspergillus fumigatus CBH II, e.g., the one disclosed as SEQ ID NO: 18 in WO 201 1/057140 and as SEQ ID NO:
- cellulolytic enzyme composition comprised in an enzyme composition of the invention, comprises one or more of the following components
- the Aspergillus fumigatus beta-glucosidase comprises one or more substitutions selected from the group consisting of L89M, G91 L, F100D, 1140V, 1186V, S283G, N456E, and F512Y; such as a variant thereof, with the following substitutions:
- the cellulolytic composition further comprises the Penicillium sp. GH61 polypeptide shown in SEQ ID NO: 10 herein; or a GH61 polypeptide comprising an amino acid sequence having at least 60%, such as at least 70%, e.g., at least 75%, at least 15 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 10 herein.
- the enzyme composition of the invention comprises a glucoamylase.
- the glucoamylase may be of any origin, such as of bacterial or fungal origin.
- the glucoamylase may be one derived from a strain of Trametes, such as a strain of Trametes cingulata (SEQ ID NO: 12 herein); or a strain of Pachykytospora, such as a strain of Pachykytospora papyracea; or a strain of Leucopaxillus, 25 such as a strain of Leucopaxillus giganteus fall disclosed in WO 2006/069289).
- a strain of Trametes such as a strain of Trametes cingulata (SEQ ID NO: 12 herein)
- a strain of Pachykytospora such as a strain of Pachykytospora papyracea
- a strain of Leucopaxillus, 25 such as a strain of Leucopaxillus giganteus fall disclosed in WO 2006/069289.
- the glucoamylase comprised in an enzyme composition of the invention, is derived from a strain of Trametes cingulata, such as one selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 12 herein;
- glucoamylase comprising an amino acid sequence having at least 60%, at least
- the glucoamylase comprised in an enzyme composition of the 35 invention, is from a strain of Aspergillus, preferably Aspergillus niger, Aspergillus awamori, or Aspergillus oryzae; or a strain of Trichoderma, preferably Trichoderma reesei; or a strain of Talaromyces, preferably Talaromyces emersonii (SEQ ID NO: 1 1 herein).
- glucoamylase such as one derived from a strain of Talaromyces emersonii, is selected from the group consisting of:
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 1 1 herein.
- the glucoamylase is derived from a strain of Penicillium, such as a strain of Penicillium oxalicum.
- glucoamylase such as one derived from a strain of Penicillium oxalicum, is selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 16 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 16 herein.
- the glucoamylase is derived from a strain of Gloeophyllum, such
- glucoamylase 20 as a strain of Gloeophyllum sepiarium or Gloeophyllum trabeum, such as one disclosed in WO 201 1/068803 as any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14 or 16.
- the glucoamylase is SEQ ID NO: 2 in WO 201 1/068803 or SEQ ID NO: 4 herein.
- the glucoamylase is SEQ ID NO: 18 in WO 201 1/068803
- glucoamylase such as one derived from a strain of
- Gloeophyllum sepiarium is selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 4 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- the glucoamylase is derived from a strain of the genus Pycnoporus, in particular a strain of Pycnoporus sanguineus, such as a strain described in WO 201 1/066576 (SEQ ID NOs 2, 4 or 6).
- the glucoamylase is the one shown in SEQ ID NO: 4 in WO 201 1/066576 or SEQ ID NO: 17 herein.
- the glucoamylase such as one derived from a strain of Pycnoporus sanguineus, is selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 17 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%,
- glucoamylases which exhibit a high identity to any of the above-mentioned glucoamylases, i.e., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, such as 10 100% identity to any one of the mature parts of the enzyme sequences mentioned above.
- glucoamylase such as one derived from a strain of Gloeophyllum trabeum, is selected from the group consisting of:
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 18 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 15 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- ID NO: 18 has one of the following substitutions: V59A; S95P; A121 P; T1 19W; 20 S95P+A121 P; V59A+S95P; S95P+T1 19W; V59A+S95P+A121 P; or S95P+T1 19W+A121 P, especially S95P+A121 P.
- the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 has one of the following substitutions: V59A; S95P;
- S95P+T1 19W+A121 P especially S95P+A121 P (using SEQ ID NO: 18 herein for 25 numbering).
- All Gloeophyllum trabeum glucoamylase variants disclosed in co-pending application EP13165995.5 and PCT/EP2014/058692 are hereby incorporated by reference.
- a variant comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least
- the enzyme composition of the invention comprises an alpha-amylase.
- the alpha- amylase may be of any origin, such as of fungal or bacterial origin.
- the alpha-amylase is an acid alpha-amylase, i.e., having a pH optimum below pH 7.
- the alpha-amylase may be derived from a strain of the genus Rhizomucor, preferably a strain the Rhizomucor pusillus, such as the one shown in SEQ ID NO: 3 in WO 2013/006756 (see e.g., Table 1 in Example 1 hereby incorporated by reference), or the genus Meripilus, preferably a strain of Meripilus giganteus.
- the alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), disclosed as V039 in Table 5 in WO 2006/069290 (incorporated by reference) or SEQ ID NO: 13 herein.
- SBD starch-binding domain
- the alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), disclosed in WO 2013/006756 (incorporated by reference) or SEQ ID NO: 13 herein.
- SBD Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain
- the Rhizomucor pusillus alpha-amylase or the Rhizomucor pusillus alpha-amylase with an Aspergillus niger glucoamylase linker and starch-binding domain has at least one of the following substitutions or combinations of substitutions: D165M; Y141W; Y141 R; K136F; K192R; P224A; P224R; S123H+Y141W; G20S + Y141W; A76G + Y141W; G128D + Y141 W; G128D + D143N; P219C + Y141W; N142D + D143N; Y141W + K192R; Y141W + D143N; Y141W + N383R; Y141W + P219C + A265C; Y141W + N142D + D143N; Y141W + K192R V410A; G128D + Y141W + D143N; Y141W + K192R
- Rhizomucor pusillus alpha-amylase with an Aspergillus niger glucoamylase linker and starch-binding domain is selected from the group consisting of:
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 13 herein.
- the alpha-amylase is a variant of the Rhizomucor pusillus alpha-amylase with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), wherein the alpha-amylase variant comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity, but less than 100% to the mature polypeptide of SEQ ID NO: 13 herein.
- SBD starch-binding domain
- the alpha-amylase variant has one of the above mentioned substitutions, such as: G128D, Y141W, D143W or K192R.
- the alpha-amylase (using SEQ ID NO: 13 herein for numbering) has the following substitutions: Y141 W+D143N.
- the alpha-amylase has the following substitutions: G128D+Y141W+D143N.
- the alpha-amylase has the following substitutions: G128D+Y141W+D143N+K192R;
- a variant comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 13 herein.
- An enzyme composition of the invention may optionally comprise a protease.
- the protease may be of any origin, such as fungal or bacterial origin.
- the protease is a metallo protease.
- the protease is a serine protease.
- the protease is of fungal origin.
- the protease is a metallo protease derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- the protease such as one derived from a strain of Thermoascus aurantiacus, is selected from the group consisting of:
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 3 herein.
- the protease is of bacterial origin.
- the protease, optionally comprised in an enzyme composition of the invention is derived from a strain of Pyrococcus, such as a strain of Pyrococcus furiosus, such as the protease shown in SEQ ID NO: 1 in US 6,358,726 or SEQ ID NO: 5 herein.
- the protease such as one derived from Pyrococcus furiosus, is 5 selected from the group consisting of:
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- the protease is a serine protease, such as a peptidase family S53 protease.
- Serine proteases of the peptidase family S53 comprises two different types of peptidases: tripeptidyl aminopeptidases (exo-type) and endo-peptidases; as described in 1993, Biochem.
- the protease is a peptidase family S53 protease derived from a strain of Meripilus, preferably a strain of Meripilus giganteus. In an embodiment the protease
- protease 20 is the mature sequence from Meripilus giganteus protease 3 (peptidase family S53 protease) concerned in Example 2 in WO 2014/037438 and shown as SEQ ID NO: 20 herein.
- the protease is the mature protease 3 sequence from Meripilus giganteus shown as SEQ ID NO: 19 herein and SEQ ID NO: 5 in WO 2014/037438.
- the protease such as Meripilus giganteus protease 3 is
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- the protease such as Meripilus giganteus protease 3, is selected from the group consisting of:
- a protease comprising an amino acid sequence having at least 60%, at least 70%, 35 e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 20 herein.
- An enzyme composition of the invention may optionally comprise a pullulanase.
- the pullulanase may be of any origin, such as fungal or bacterial origin.
- the pullulanase optionally comprised in an enzyme composition of the invention is derived from a strain of Bacillus sp. such as the one shown in SEQ ID NO: 15 herein or a strain of Bacillus deramificans.
- the pullulanase such as one derived from Bacillus sp, is selected from the group consisting of:
- a pullulanase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 15 herein.
- the enzyme composition of the invention further comprising a pullulanase, such as a Bacillus sp. pullulanase and a Talaromyces emersonii glucoamylase and/or Gloeophyllum sepiarium glucoamylase.
- a pullulanase such as a Bacillus sp. pullulanase and a Talaromyces emersonii glucoamylase and/or Gloeophyllum sepiarium glucoamylase.
- the enzyme composition may further comprise a trehalase.
- the trehalase may be of any origin, such as fungal or bacterial origin.
- the trehalase is of fungal origin, such as derived from a strain of Trichoderma, such as Trichoderma reesei, such as the one shown in SEQ ID NO: 14 herein.
- the trehalase such as one derived from Trichoderma reesei, is selected from the group consisting of:
- a trehalase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 14 herein.
- the enzyme composition may further comprise a pectinase, such as a pectin lyase (also known as pectolyase) and/or a polygalacturonase, or a combination thereof.
- a pectinase such as a pectin lyase (also known as pectolyase) and/or a polygalacturonase, or a combination thereof.
- the pectinase may be of any origin, such as fungal or bacterial origin.
- the pectinase is a pectin lyase (EC 4.2.2.10).
- the pectin lyase is derived from a strain of Aspergillus, such as
- the pectinase is a polygalacturonase (EC. 3.2.1.15).
- polygalacturonase is derived from a strain of Aspergillus, such as Aspergillus aculeatus.
- the pectinase is a combination of pectin lyase and polygalacturonase. In an embodiment the pectinase is a combination of pectin lyase derived from Aspergillus niger and polygalacturonase derived from Aspergillus aculeatus.
- the enzyme composition of the invention comprises a fungal glucoamylase and a fungal alpha-amylase, and optionally a protease.
- the enzyme composition comprises the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein having one or more of the following substitutions: S95P, A121 P, preferably S95P+A121 P, and an alpha-amylase, preferably an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, having one or more of the following substitutions: G128D, D143N, preferably G128D+D143N.
- SBD starch-binding domain
- the enzyme composition comprises the Pycnoporus sanguineus glucoamylase shown in SEQ ID NO: 17 herein; and an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably the one shown in SEQ ID NO: 13 herein, preferably having one or more of the following substitutions: G128D, D143N, especially G128D+D143N.
- SBD starch-binding domain
- the enzyme composition comprises the Gloeophyllum sepiarium glucoamylase shown in SEQ ID NO: 4 herein, and an alpha-amylase, preferably an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, having one or more of the following substitutions: G128D, D143N, preferably G128D+D143N.
- SBD starch-binding domain
- the enzyme composition comprises the Trametes cingulata glucoamylase shown in SEQ ID NO: 1 1 herein, and an alpha-amylase, preferably an alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, having one or more of the following substitutions: G128D, D143N, preferably G128D+D143N.
- SBD starch-binding domain
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising a GH61 polypeptide, beta-glucosidase, CBH I and CBH II;
- Rhizomucor pusillus alpha-amylase or variant thereof
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- iv) optionally a protease from Thermoascus aurantiacus, or variant thereof.
- Rhizomucor pusillus alpha-amylase or variant thereof
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y (using SEQ ID NO: 8 herein for numbering), and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- iv) optionally a protease from Pyropoccus furiosus, preferably the one shown in .
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei;
- protease from Thermoascus aurantiacus, or a variant thereof and/or Pyrococcus furiosus, or Meripilus giganteus.
- fungal alpha-amylase iii) fungal alpha-amylase; iii) cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising a GH61 polypeptide, beta-glucosidase CBH I and CBH II;
- pectinase preferably a pectin lyase or a polygalacturonase, or a combination thereof.
- the pectinase is a combination of pectin lyase and polygalacturonase. In an embodiment the pectinase is a combination of pectin lyase derived from Aspergillus niger and polygalacturonase derived from Aspergillus aculeatus.
- pectinase preferably a pectin lyase or a polygalacturonase, or a combination thereof;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising a GH61 polypeptide, beta-glucosidase CBH I and CBH II;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising a GH61 polypeptide, beta-glucosidase CBH I and CBH II;
- Rhizomucor pusillus alpha-amylase or variant thereof
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- pectin lyase derived from Aspergillus niger or polygalacturonase derived from Aspergillus aculeatus, or a combination thereof;
- protease from Thermoascus aurantiacus, or a variant thereof, or Pyrococcus furiosus, or Meripilus giganteus.
- the enzyme composition comprises i) Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein having one or more of the following substitutions: S95P+A121 P;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- protease from Thermoascus aurantiacus, or a variant thereof.
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- protease from Thermoascus aurantiacus, or a variant thereof.
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta- glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- Meripipus giganteus optionally iv) protease from Meripipus giganteus, such as Meripipus giganteus protease 3.
- a cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta-glucosidase with the following substitutions: F100D, S283G, N456E, F512Y (sing SEQ ID NO: 8 herein for numbering), and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- protease from Meripipus giganteus such as Meripipus giganteus protease 3, or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 20 herein.
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, or an alpha-amylasehaving at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 13 herein, preferably having of the following substitutions: G128D+D143N;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, preferably further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta-glucosidase with the following substitutions: F100D, S283G, N456E, F512Y (using SEQ ID NO: 8 for numbering), and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- protease from Thermoascus aurantiacus shown in SEQ ID NO: 3 herein, or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 3 herein, or a variant thereof.
- glucoamylase shown in SEQ ID NO: 12 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least
- SEQ ID NO: 12 5 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 12 herein; ii) alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least
- SBD starch-binding domain
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, preferably further comprising Penicillium emersonii GH61A polypeptide, Aspergillus
- fumigatus beta-glucosidase with the following substitutions: F100D, S283G, N456E, F512Y (using SEQ ID NO: 8 herein for numbering), and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- protease from Thermoascus aurantiacus, or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 20 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 3 herein, or a variant thereof.
- an enzyme composition of the invention may suitably be used in
- RSH raw starch hydrolysis
- the desired fermentation product may in an embodiment be ethanol produced from un-gelatinized (i.e., uncooked), preferably milled, grains, such as corn, or small grains such
- starch-Containing Materials' -section examples of suitable starch-containing starting materials are listed in the section "Starch-Containing Materials' -section below.
- the invention relates to processes of producing fermentation products from starch-containing material comprising:
- saccharification and/or fermentation is done in the presence of the following enzymes: glucoamylase and alpha-amylase, and optionally a protease.
- the glucoamylase is the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein having one or more of the following substitutions: S95P, A121 P, preferably S95P+A121 P and the alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), shown in SEQ ID NO: 13 herein, having one or more of the following substitutions: G128D, D143N, preferably G128D+D143N.
- SBD starch-binding domain
- the enzyme may be added as an enzyme composition of the invention.
- steps (a) and (b) are carried out simultaneously (i.e., one-step fermentation). However, step (a) and (b) may also be carried our sequentially.
- a desired fermentation product such as ethanol
- a desired fermentation product such as ethanol
- the process of the invention includes saccharifying (e.g., milled) starch- containing material, e.g., granular starch, below the initial gelatinization temperature, preferably in the presence of an enzyme composition of the invention.
- saccharifying e.g., milled
- starch- containing material e.g., granular starch
- a cellulolytic enzyme composition is one described above in the "Cellulolytic Enzyme Composition"-section.
- the cellulolytic enzyme composition is added to the process of the invention.
- the cellulolytic enzyme composition is comprised in an enzyme composition of the invention.
- the enzymes preferably in the form of an enzyme composition of the invention, are added to the saccharification and/or fermentation, preferably simultaneous saccharification and fermentation. It should be understood that the enzymes may also be added individually or as two, three, four or more enzyme composition.
- the glucoamylase and alpha-amylase are added as one blend composition and the cellulolytic enzyme composition and optional protease are added separately.
- the cellulolytic enzyme composition, the glucoamylase, and the alpha-amylase are added as one enzyme composition and the optional protease is added separately.
- All enzymes may be added as one enzyme composition comprising a glucoamylase, an alpha-amylase, optionally a cellulolytic enzyme composition, and/or a protease, and optionally other enzymes including pullulanase and/or pectinase, such as pectin lyase or polygalacturonase.
- the fermentation product such as especially a liquid fermentation product, such as ethanol
- a liquid fermentation product such as ethanol
- the fermentation product may optionally be recovered after fermentation, e.g., by distillation. Subsequent to fermentation the fermentation product may be separated from the fermentation medium.
- the fermentation medium may be distilled to extract the desired fermentation product or the desired fermentation product may be extracted from the fermentation medium by micro or membrane filtration techniques. Alternatively, the fermentation product may be recovered by stripping. Methods for recovering fermentation products are well known in the art.
- yeast preferably a strain of Saccharomyces cerevisiae. Saccharomyces cerevisiae may according to the invention be used for producing ethanol.
- Other suitable fermenting organisms are listed in the "Fermenting Organisms"- section below.
- initial gelatinization temperature means the lowest temperature at which starch gelatinization commences. In general, starch heated in water begins to gelatinize between about 50°C and 75°C. The exact temperature of gelatinization depends on the specific starch and depends on the degree of cross-linking of the amylopectin.
- the initial gelatinization temperature can readily be determined by the skilled artisan. The initial gelatinization temperature may vary according to the plant species, to the particular variety of the plant species as well as with the growth conditions. In context of this invention the initial gelatinization temperature of a given starch-containing material may be determined as the temperature at which birefringence is lost in 5% of the starch granules using the method described by Gorinstein. S. and Lii. C, Starch/Starke, Vol. 44 (12) pp. 461-466 (1992).
- an aqueous slurry of starch-containing material such as granular starch, having 10-55 wt.-% dry solids (DS), preferably 25-45 wt.-% dry solids, more preferably 30-40% dry solids of starch-containing material may be prepared.
- the slurry may include water and/or process waters, such as stillage (backset), scrubber water, evaporator condensate or distillate, side-stripper water from distillation, or process water from other fermentation product plants. Because the process of the invention is carried out below the 5 initial gelatinization temperature and thus no significant viscosity increase takes place, high levels of stillage may be used, if desired.
- the aqueous slurry contains from about 1 to about 70 vol.-%, preferably 15-60% vol.-%, especially from about 30 to 50 vol.-% water and/or process waters, such as stillage (backset), scrubber water, evaporator condensate or distillate, side-stripper water from distillation, or process water from other
- backset or another recycled stream, is added to the slurry before step (a), or to the saccharification (step (a)), or to the simultaneous saccharification and fermentation steps (combined step (a) and step (b)).
- a process of the invention is conducted at a temperature below the initial 20 gelatinization temperature, which means that the temperature at which a separate step (a) is carried out typically lies in the range between 25-75°C, such as between 30-70°C, or between 45-60°C.
- the temperature during fermentation in step (b) or simultaneous saccharification and fermentation in steps (a) and (b) is between 25°C and 25 40°C, preferably between 28°C and 36°C, such as between 28°C and 35°C, such as between 28°C and 34°C, such as around 32°C.
- fermentation is carried out for 30 to 150 hours, preferably 48 to 96 hours. 66.
- fermentation is carried out so that the sugar level, such as glucose 30 level, is kept at a low level, such as below 6 wt.-%, such as below about 3 wt.-%, such as below about 2 wt.-%,such as below about 1 wt.-%., such as below about 0.5%, or below 0.25% wt.-%, such as below about 0.1 wt.-%.
- a low level of sugar can be accomplished by simply employing adjusted quantities of enzymes and fermenting organism. A skilled person in the art can easily determine which doses/quantities of enzyme and fermenting 35 organism to use.
- the employed quantities of enzyme and fermenting organism may also be selected to maintain low concentrations of maltose in the fermentation broth. For instance, the maltose level may be kept below about 0.5 wt.-%, such as below about 0.2 wt.-%.
- the process of the invention may be carried out at a pH from 3 and 7, preferably from 3 to 6, or more preferably from 3.5 to 5.0.
- granular starch means raw uncooked starch, i.e., starch in its natural form found in, e.g., cereal, tubers or grains. Starch is formed within plant cells as tiny granules insoluble in water. When put in cold water, the starch granules may absorb a small amount of the liquid and swell. At temperatures up to around 50°C to 75°C the swelling may be reversible. However, at higher temperatures an irreversible swelling called “gelatinization" begins.
- the granular starch may be a highly refined starch, preferably at least 90%, at least 95%, at least 97% or at least 99.5% pure, or it may be a more crude starch-containing materials comprising (e.g., milled) whole grains including non-starch fractions such as germ residues and fibers.
- the raw material such as whole grains, may be reduced in particle size, e.g., by milling, in order to open up the structure and allowing for further processing.
- suitable particle sizes are disclosed in US4514496 and WO2004/081 193 (incorporated by reference).
- Two processes are preferred according to the invention: wet and dry milling. In dry milling whole kernels are milled and used. Wet milling gives a good separation of germ and meal (starch granules and protein) and is often applied at locations where the starch hydrolysate is used in production of, e.g., syrups. Both dry and wet milling is well known in the art of starch processing.
- the particle size is reduced to between 0.05 to 3.0 mm, preferably 0.1 -0.5 mm, or so that at least 30%, preferably at least 50%, more preferably at least 70%, even more preferably at least 90% of the starch-containing material fit through a sieve with a 0.05 to 3.0 mm screen, preferably 0.1-0.5 mm screen.
- starch- containing material is prepared by reducing the particle size of the starch-containing material, preferably by milling, such that at least 50% of the starch-containing material has a particle size of 0.1-0.5 mm.
- the enzymes or enzyme composition is added so that the glucoamylase is present in an amount of 0.001 to 10 AGU/g DS, preferably from 0.01 to 5 AGU/g DS, especially 0.1 to 0.5 AGU/g DS.
- the enzymes or enzyme composition is added so that the alpha-amylase is present or added in an amount of 0.001 to 10 AFAU/g DS, preferably from 0.01 to 5 AFAU/g DS, especially 0.3 to 2 AFAU/g DS or 0.001 to 1 FAU-F/g DS, preferably 0.01 to 1 FAU-F/g DS.
- the enzymes or enzyme composition is added so that the cellulolytic enzyme composition is present or added in an amount 1 -10,000 micro grams EP/g DS, such as 2-5,000, such as 3 and 1 ,000, such as 4 and 500 micro grams EP/g DS.
- the enzymes or enzyme composition is added so that the cellulolytic enzyme composition is present or added in an amount in the range from 0.1-100 FPU per gram total solids (TS), preferably 0.5-50 FPU per gram TS, especially 1-20 FPU per gram TS.
- TS FPU per gram total solids
- the enzymes or enzyme composition is added so that the protease is present in an amount of 0.0001 -1 mg enzyme protein per g DS, preferably 0.001 to 0.1 mg enzyme protein per g DS.
- the protease is present in an amount of 0.0001 to 1 LAPU/g DS, preferably 0.001 to 0.1 LAPU/g DS and/or 0.0001 to 1 mAU-RH/g DS, preferably 0.001 to 0.1 mAU-RH/g DS.
- the enzymes or enzyme composition is added so that the protease is present or added in an amount in the range 1-1 ,000 ⁇ g EP/g DS, such as 2-500 g EP/g DS, such as 3-250 EP/g DS.
- ratio between glucoamylase and alpha-amylase is between 99:1 and 1 :2, such as between 98:2 and 1 :1 , such as between 97:3 and 2:1 , such as between 96:4 and 3:1 , such as 97:3, 96:4, 95:5, 94:6, 93:7, 90:10, 85:15, 83:17 or 65:35
- the total dose of glucoamylase and alpha-amylase is according to the invention from 10-1 ,000 g/g DS, such as from 50-500 g/g DS, such as 75-
- the total dose of cellulolytic enzyme composition added is from 10-500 ⁇ g/g DS, such as from 20-400 ⁇ g/g DS, such as 20-300 ⁇ g/g DS.
- the dose of protease added is from 1 -200 g/g DS, such as from 2-
- any suitable starch-containing starting material including granular starch (raw uncooked starch), may be used.
- the starting material is generally selected based on the desired fermentation product.
- starch- containing starting materials suitable for use in processes of the present invention, include cereal, tubers or grains.
- the starch-containing material may be corn, wheat, barley, rye, milo, sago, cassava, tapioca, sorghum, rice, peas, beans, or sweet potatoes, or mixtures thereof. Contemplated are also waxy and non-waxy types of corn and barley.
- the starch- -containing starting material is corn.
- starch- -containing starting material is wheat.
- starch- -containing starting material is barley.
- starch- -containing starting material is rye.
- starch- -containing starting material is milo.
- starch- -containing starting material is sago.
- starch- -containing starting material is cassava.
- starch- -containing starting material is tapioca.
- starch- -containing starting material is sorghum.
- starch- -containing starting material is rice
- starch- -containing starting material is peas.
- starch- -containing starting material is beans.
- fermenting organism refers to any organism, including bacterial and fungal organisms, suitable for use in a fermentation process and capable of producing desired a fermentation product.
- suitable fermenting organisms are able to ferment, i.e., convert, sugars, such as glucose or maltose, directly or indirectly into the desired fermentation product, especially ethanol.
- Examples of fermenting organisms include fungal organisms, such as yeast.
- Preferred yeast includes strains of Saccharomyces spp., in particular, Saccharomyces cerevisiae.
- Fermenting organisms engineered to produce one or more enzymes such a saccharolytic enzyme, such as glucoamylase, is also contemplated.
- the contemplated yeast especially Saccharomyces cerevisae, may also be engineered to produce less glycerol. Examples of such yeast can be found in WO/201 1/153516 (Mascoma).
- the fermenting organism is added to the fermentation medium so that the viable fermenting organism, such as yeast, count per mL of fermentation medium is in the range from 10 5 to 10 12 , preferably from 10 7 to 10 10 , especially about 5x10 7 .
- yeast includes, e.g., RED STARTM and ETHANOL REDD ye ast (available from Fermentis/Lesaffre, USA), FALI (available from Fleischmann's Yeast, USA), SUPERSTART and THERMOSACCTM fresh yeast (available from Ethanol Technology, Wl, USA), BIOFERM AFT and XR (available from NABC - North American Bioproducts Corporation, GA, USA), GERT STRAND (available from Gert Strand AB, Sweden), and FERMIOL (available from DSM Specialties). Fermentation Products
- Fermentation product means a product produced by a process, including a fermentation step, of the invention using one or more fermenting organisms.
- Fermentation products contemplated according to the invention include alcohols (e.g., ethanol, methanol, butanol); organic acids (e.g., citric acid, acetic acid, itaconic acid, lactic acid, succinic acid, gluconic acid); ketones (e.g., acetone); amino acids (e.g., glutamic acid); gases (e.g., H 2 and C0 2 ); antibiotics (e.g., penicillin and tetracycline); enzymes; vitamins (e.g., riboflavin, B 12 , beta-carotene); and hormones.
- alcohols e.g., ethanol, methanol, butanol
- organic acids e.g., citric acid, acetic acid, itaconic acid, lactic acid, succinic acid, gluconic acid
- the fermentation product is ethanol, e.g., fuel ethanol; drinking ethanol, i.e., potable neutral spirits; or industrial ethanol or products used in the consumable alcohol industry (e.g., beer and wine), dairy industry (e.g., fermented dairy products), leather industry and tobacco industry.
- Preferred beer types comprise ales, stouts, porters, lagers, bitters, malt liquors, happoushu, high-alcohol beer, low-alcohol beer, low-calorie beer or light beer.
- the fermentation product, such as ethanol, obtained according to the invention may preferably be used as fuel. However, in the case of ethanol it may also be used as potable ethanol.
- fermentation medium refers to the environment in which fermentation is carried out and which includes the fermentable substrate, that is, a carbohydrate source (e.g., glucose) that can be metabolized by the fermenting organism(s).
- a carbohydrate source e.g., glucose
- the fermentation medium may comprise nutrients and/or growth stimulator(s) for the fermenting organism(s).
- Nutrient and growth stimulators are widely used in the art of fermentation and include nitrogen sources, such as ammonia; vitamins; and minerals, or combinations thereof.
- glucoamylase derived from Trametes cingulata, Gloeophyllum trabeum,
- Gloeophyllum sepiarium, or Pycnoporus sanguineus ii) alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof;
- protease from Thermoascus aurantiacus, or a variant thereof and/or Pyrococcus furiosus or Meripilus giganteus.
- glucoamylase derived from Gloeophyllum trabeum disclosed in SEQ ID NO: 18 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18 herein, preferably with the following substitutions: S95P+A121 P;
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof, shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 13 herein, preferably with the following substitutions: G128D+D143N;
- protease from Thermoascus aurantiacus, or a protease having at least
- At least 70% e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 3 herein, or a variant thereof.
- glucoamylase derived from Gloeophyllum trabeum disclosed in SEQ ID NO: 18 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof, shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%,
- a cellulolytic enzyme composition such as one derived from Trichoderma reeser ' ,
- a protease from Meripilus giganteus such as Meripilus giganteus protease 3, or a 15 protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 20 herein.
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof, shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO:
- a protease from Meripilus giganteus such as Meripilus giganteus protease 3, such as one disclosed in SEQ ID NO: 19 herein or SEQ ID NO: 20 herein, or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least
- glucoamylase derived from Pycnoporus sanguineus shown in SEQ ID NO: 17 herein; or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 17 herein,
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof, shown in SEQ ID NO: 13 herein, or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO:
- iv) optionally a protease from Thermoascus aurantiacus, or a variant thereof.
- SBD
- a protease from Thermoascus aurantiacus or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 3 herein, or a variant thereof.
- a protease from Thermoascus aurantiacus or a protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 3 herein, or a variant thereof.
- GtAMG Glucoamylase derived from Gloeophyllum trabeum disclosed in SEQ ID NO: 18 herein, with the following substitutions: S95P+A121 P.
- PsAMG Glucoamylase derived from Pycnoporus sanguineus disclosed as shown in SEQ ID NO: 4 in WO 201 1/066576 and in SEQ ID NO: 17 herein.
- TcAMG Glucoamylase derived from Trametes cingulata shown in SEQ ID NO: 12 herein or SEQ ID NO: 2 in WO 2006/69289
- AAPE096 Alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD) shown in SEQ I D NO: 13 herein, with the following substitutions: G128D+D143N.
- Cellulase VP Cellulolytic composition derived from Trichoderma reesei further comprising Penicillium emersonii GH61A polypeptide disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, and Aspergillus fumigatus beta-glucosidase disclosed as SEQ ID NO: 2 in WO 2005/047499 or SEQ ID NO: 8 herein, or a variant thereof, preferably a variant having one of, preferably all of, the following substitutions: F100D, S283G, N456E, F512Y and Aspergillus fumigatus Cel7A CBH1 disclosed as SEQ ID NO: 6 in WO201 1/057140 and SEQ ID NO: 6 herein and Aspergillus fumigatus CBH II disclosed as SEQ ID NO: 18 in WO 201 1/057140 and as SEQ ID NO: 7 herein.
- Protease Oxa Metallo protease derived from Thermoascus aurantiacus disclosed as the mature part of SEQ ID NO: 2 disclosed in WO 2003/048353 or the mature polypeptide of SEQ ID NO: 3 herein.
- Mg Protease 3 Serine peptidase family S53 protease from a strain of Meripilus giganteus concerned in Example 2 in WO 2014/037438 or SEQ ID NO: 20.
- One SIGMA unit will convert 1.0 micro mol of trehalose to 2.0 micro mol of glucose per minutes at pH 5.7 at 37°C (liberated glucose determined at pH 7.5).
- Glucoamylase activity may be measured in Glucoamylase Units (AGU).
- the Novo Glucoamylase Unit is defined as the amount of enzyme, which hydrolyzes 1 micromole maltose per minute under the standard conditions 37°C, pH 4.3, substrate: maltose 23.2 mM, buffer: acetate 0.1 M, reaction time 5 minutes.
- An autoanalyzer system may be used. Mutarotase is added to the glucose dehydrogenase reagent so that any alpha-D-glucose present is turned into beta-D-glucose. Glucose dehydrogenase reacts specifically with beta-D-glucose in the reaction mentioned above, forming NADH which is determined using a photometer at 340 nm as a measure of the original glucose concentration.
- Enzyme working range 0.5-4.0 AGU/mL
- KNU Alpha-amylase activity
- the alpha-amylase activity may be determined using potato starch as substrate. This method is based on the break-down of modified potato starch by the enzyme, and the reaction is followed by mixing samples of the starch/enzyme solution with an iodine solution. Initially, a blackish-blue color is formed, but during the break-down of the starch the blue color gets weaker and gradually turns into a reddish-brown, which is compared to a colored glass standard.
- KNU Kilo Novo alpha amylase Unit
- an acid alpha-amylase When used according to the present invention the activity of an acid alpha-amylase may be measured in AFAU (Acid Fungal Alpha-amylase Units) or FAU-F. Acid alpha-amylase activity (AFAU)
- AFAU Acid alpha-amylase activity
- Acid alpha-amylase activity may be measured in AFAU (Acid Fungal Alpha-amylase Units), which are determined relative to an enzyme standard. 1 AFAU is defined as the amount of enzyme which degrades 5.260 mg starch dry matter per hour under the below mentioned standard conditions.
- Acid alpha-amylase an endo-alpha-amylase (1 ,4-alpha-D-glucan-glucanohydrolase, E.C. 3.2.1.1 ) hydrolyzes alpha-1 ,4-glucosidic bonds in the inner regions of the starch molecule to form dextrins and oligosaccharides with different chain lengths.
- the intensity of color formed with iodine is directly proportional to the concentration of starch.
- Amylase activity is determined using reverse colorimetry as a reduction in the concentration of starch under the specified analytical conditions.
- IODINE 40 pH 2,5 > DEXTRINS + OLIGOSACCHARIDES
- Iodine (I2) 0.03 g/L
- FAU-F Fungal Alpha-Amylase Units (Fungamyl) is measured relative to an enzyme standard of a declared strength. Reaction conditions
- NREL Renewable Energy Laboratory
- a rolled filter paper strip (#1 Whatman; 1 X 6 cm; 50 mg) is added to the bottom of a test tube (13 X 100 mm).
- Enzyme dilutions are designed to produce values slightly above and below the target value of 2.0 mg glucose.
- the tubes are incubated for 60 mins. at 50° C ( ⁇ 0.1 ° C) in a circulating water bath. 2.2.7 Immediately following the 60 min. incubation, the tubes are removed from the water bath, and 3.0 mL of DNS reagent is added to each tube to stop the reaction. The tubes are vortexed 3 seconds to mix.
- a reagent blank is prepared by adding 1.5 mL of citrate buffer to a test tube.
- a substrate control is prepared by placing a rolled filter paper strip into the bottom of a test tube, and adding 1.5 mL of citrate buffer.
- Enzyme controls are prepared for each enzyme dilution by mixing 1 .0 mL of citrate buffer with 0.5 mL of the appropriate enzyme dilution.
- Glucose standard tubes are prepared by adding 0.5 mL of each dilution to 1.0 mL of citrate buffer.
- glucose standard tubes are assayed in the same manner as the enzyme assay tubes, and done along with them.
- each tube is diluted by adding 50 microL from the tube to 200 microL of ddH20 in a 96-well plate. Each well is mixed, and the absorbance is read at 540 nm.
- a glucose standard curve is prepared by graphing glucose concentration (mg/0.5 mL) for the four standards (G1 -G4) vs. A 540 . This is fitted using a linear regression (Prism Software), and the equation for the line is used to determine the glucose produced for each of the enzyme assay tubes.
- a plot of glucose produced (mg/0.5 ml.) vs. total enzyme dilution is prepared, with the Y-axis (enzyme dilution) being on a log scale.
- the proteolytic activity may be determined with denatured hemoglobin as substrate.
- denatured hemoglobin is digested, and the undigested hemoglobin is precipitated with trichloroacetic acid
- TCA TCA soluble product
- One Anson Unit is defined as the amount of enzyme which under standard conditions (i.e. 25°C, pH 5.5 and 10 min. reaction time) digests hemoglobin at an initial rate such that there is liberated per minute an amount of TCA soluble product which gives the same color with phenol reagent as one milliequivalent of tyrosine.
- the AU(RH) method is described in EAL-SM-0350 and is available from Novozymes A/S Denmark on request.
- LAPU Protease assay method
- LAPU 1 Leucine Amino Peptidase Unit
- LAPU is described in EB-SM-0298.02/01 available from Novozymes A/S Denmark on request.
- MANU J altogenic Amylase Novo Unit
- Finely-ground (250 microns) raw starch slurry was generated for this experiment.
- Approximately 405 g yellow dent corn flour obtained from Southeast Iowa Renewable Energy, IA, USA - ground in-house
- DS dry solids
- the mash was prepared to 500 ppm urea and 3 mg/L penicillin using solutions of 200 g/L urea and 1 g/L penicillin, respectively, and adjusted to pH 4.5.
- Approximately 5 g of the prepared mashes were pipetted into each of preweighed 15 mL centrifuge tubes, which had holes drilled in the top. The tubes were again weighed to determine the mass of mash added.
- RED STARTM yeast was rehydrated, with 5.5 g of yeast placed in 100 mL of 32°C tap water for 30 minutes. While the yeast soaked, each mash sample was dosed with glucoamylase (AMG) and alpha-amylase (AA) enzymes in the amounts and ratios according to the tables below.
- AMG glucoamylase
- AA alpha-amylase
- Enzyme dosage was calculated using the following equation:
- the method quantifies analytes using calibration standards for dextrins (DP4+), maltotriose, maltose, glucose, fructose, acetic acid, lactic acid, glycerol and ethanol.
- a 4 point calibration including the origin is used.
- the AMG and AA ratio was held constant at 94:6 protein ratio, respectively, at 147.9 ⁇ g total protein dose. Additional enzyme(s) in the amounts indicated were added in separate treatments and the results obtained according to the Table below.
- the AMG and AA ratio was held constant at 77:23 protein ratio, respectively, at 1 16 total protein dose. Additional enzyme(s) in the amounts indicated were added in separate treatments and the results obtained according to the Table below.
- Finely-ground (250 microns) raw starch slurry was generated for this experiment.
- Approximately 405 g yellow dent corn flour obtained from Southeast Iowa Renewable Energy, IA, USA - ground in-house
- DS dry solids
- the mash was prepared to 500 ppm urea and 3 mg/L penicillin using solutions of 200 g/L urea and 1 g/L penicillin, respectively, and adjusted to pH 4.5.
- Approximately 5 g of the prepared mashes were pipetted into each of preweighed 15 mL centrifuge tubes, which had holes drilled in the top. The tubes were again weighed to determine the mass of mash added.
- RED STARTM yeast was rehydrated, with 5.5 g of yeast placed in 100 mL of 32°C tap water for 30 minutes. While the yeast soaked, each mash sample was dosed with glucoamylase (AMG) and alpha-amylase (AA) enzymes in the amounts and ratios according to the tables below.
- AMG glucoamylase
- AA alpha-amylase
- Enzyme dosage was calculated using the following equation:
- HPLC samples were analyzed on the following system.
- the method quantifies analytes using calibration standards for dextrins (DP4+), maltotriose, maltose, glucose, fructose, acetic acid, lactic acid, glycerol and ethanol.
- a 4 point calibration including the origin is used.
- the AMG and AA ratio was held constant at 90:10 protein ratio, respectively, and the overall dose was increased as shown in the Table below.
- Cellulase VD and Protease Oxa was added to the treatments indicated. Doses are stated as ug enzyme protein per gram DS.
- Table 2 shows the enzymes used for this experiment. Water was added to each tube to bring the total added volume (enzyme + water) to 5.3% of the initial weight of the mash. This volume correction brings all tubes in the experiment to the same total percent solids, making ethanol concentrations directly comparable between treatments.
- HPLC preparation consisted of stopping the reaction by addition of 50 ⁇ of 40% H 2 S0 4 , centrifuging for 10 min at 1462xg, and filtering through a 0.45 ⁇ filter. Samples were stored at 4°C.
- the method quantifies analytes using calibration standards for DP4+, DP3, DP2, glucose, fructose, acetic acid, lactic acid, glycerol and ethanol. A four point calibration including the origin is used. Dry Solids Analysis
- AGU/FAU-F ratio AGU/FAU-F ratio
- AGU dose 49.6 33.6 21.2 AGU dose 49.6 33.6 21.2
- AGU/FAU-F ratio AGU/FAU-F ratio
- AGU dose 49.6 33.6 21.2 AGU dose 49.6 33.6 21.2
- AGU/FAU-F ratio AGU/FAU-F ratio
- AGU dose 49.6 33.6 21.2 AGU dose 49.6 33.6 21.2
- Mg Proteases aid in increasing fermentation kinetics and final overall ethanol yields.
- Mg Protease 3 significantly improves ethanol production and faster fermentation rates compared to TcAMG/PE096, PsAMG/AAPE096 (No Protease) and PsAMG/AAPE096 + Protease Oxa.
- An enzyme composition comprising glucoamylase and alpha-amylase, and optionally protease.
- the cellulolytic enzyme composition is derived from a strain of Trichoderma, such as Trichoderma reesei; a strain of Humicola, such as Humicola insolens; a strain of Chrysosporium, such as Chrysosporium lucknowense; or a strain of Penicillium, such as Penicillium decumbens.
- the cellulolytic enzyme composition comprises a beta-glucosidase, preferably one derived from a strain of the genus Aspergillus, such as Aspergillus oryzae, such as the one disclosed in WO 2002/095014 or the fusion protein having beta-glucosidase activity disclosed in WO 2008/057637, or Aspergillus fumigatus, such as one disclosed in SEQ ID NO: 2 in WO 2005/047499 or SEQ ID NO: 6 herein or an Aspergillus fumigatus beta-glucosidase variant disclosed in WO 2012/044915 ; or a strain of the genus a strain Penicillium, such as a strain of the Penicillium brasilianum disclosed in WO 2007/019442, or a strain of the genus Trichoderma, such as a strain of Trichoderma reesei.
- a strain of the genus Aspergillus oryzae such as the one disclosed in
- beta-glucosidase is from a strain of Aspergillus, such as a strain of Aspergillus fumigatus, such as Aspergillus fumigatus beta-glucosidase (SEQ ID NO: 8 herein), or a variant thereof, which variant comprises one or more substitutions selected from the group consisting of L89M, G91 L, F100D, 1140V, 1186V, 10 S283G, N456E, and F512Y; such as a variant thereof with the following substitutions:
- beta-glucosidase variant has at least 60% identity, such as at least 70%, such as at least 80%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, but less than 100% identity to mature polypeptide SEQ ID NO: 8 herein.
- the cellulolytic enzyme composition comprises a GH61 polypeptide, such as one derived from the genus Thermoascus, such as a strain of Thermoascus aurantiacus, such as the one described in WO 2005/074656 as SEQ ID NO: 2 or SEQ I D NO: 9 herein; or one derived from the genus
- a GH61 polypeptide such as one derived from the genus Thermoascus, such as a strain of Thermoascus aurantiacus, such as the one described in WO 2005/074656 as SEQ ID NO: 2 or SEQ I D NO: 9 herein; or one derived from the genus
- Thielavia such as a strain of Thielavia terrestris, such as the one described in WO 2005/074647 as SEQ ID NO: 7 and SEQ ID NO: 8; or one derived from a strain of Aspergillus, such as a strain of Aspergillus fumigatus, such as the one described in WO 2010/138754 as SEQ ID NO: 1 and SEQ ID NO: 2; or one derived from a strain derived from Penicillium, such as a strain of Penicillium emersonii, such as the one disclosed in WO
- GH61 polypeptide such as Penicillium sp.
- GH61 polypeptide is selected from the group consisting of:
- a GH61 polypeptide comprising the mature polypeptide of SEQ ID NO: 10 herein; 5 (ii) a GH61 polypeptide comprising an amino acid sequence having at least 60%, such as at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 10 herein.
- the cellulolytic enzyme composition comprises a cellobiohydrolase I (CBH I), such as one derived from a strain of the genus Aspergillus, such as a strain of Aspergillus fumigatus, such as the Cel7a CBH I disclosed in SEQ ID NO: 6 in WO 201 1/057140 or SEQ ID NO: 6 herein, or a strain of the genus Trichoderma, such as a strain of Trichoderma reesei.
- CBH I cellobiohydrolase I
- cellobiohydrolase I such as Aspergillus fumigatus cellobiohydrolase I, is selected from the group consisting of:
- a cellobiohydrolase I comprising an amino acid sequence having at least 70%, e.g., 20 at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least
- the cellulolytic enzyme 25 composition comprises a cellobiohydrolase II (CBH II, such as one derived from a strain of the genus Aspergillus, such as a strain of Aspergillus fumigatus; such as the one disclosed as SEQ ID NO: 7 herein or a strain of the genus Trichoderma, such as Trichoderma reesei, or a strain of the genus Thielavia, such as a strain of Thielavia terrestris, such as cellobiohydrolase II CEL6A from Thielavia terrestris.
- CBH II cellobiohydrolase II
- cellobiohydrolase II such as Aspergillus fumigatus cellobiohydrolase II, is selected from the group consisting of:
- a cellobiohydrolase II comprising an amino acid sequence having at least 70%, e.g., 35 at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 7 herein.
- the enzyme composition of any of paragraphs 1 -16, wherein the cellulolytic enzyme 5 composition comprises a GH61 polypeptide having cellulolytic enhancing activity and a beta- glucosidase.
- the cellulolytic enzyme composition comprises a GH61 polypeptide having cellulolytic enhancing activity, a beta- glucosidase, and a CBHI.
- the cellulolytic enzyme composition comprises a GH61 polypeptide having cellulolytic enhancing activity, a beta- glucosidase, a CBHI, and a CBHII.
- the enzyme composition of any of paragraphs 1 -21 wherein the cellulolytic enzyme composition is a Trichoderma reesei cellulolytic composition, further comprising Thermoascus aurantiacus GH61A polypeptide having cellulolytic enhancing activity (SEQ ID NO: 2 in WO 2005/074656 or SEQ ID NO: 9 herein) and Aspergillus fumigatus beta- glucosidase (SEQ ID NO: 2 of WO 2005/047499) or SEQ ID NO: 8 herein.
- Thermoascus aurantiacus GH61A polypeptide having cellulolytic enhancing activity SEQ ID NO: 2 in WO 2005/074656 or SEQ ID NO: 9 herein
- Aspergillus fumigatus beta- glucosidase SEQ ID NO: 2 of WO 2005/047499
- the cellulolytic enzyme composition is a Trichoderma reesei cellulolytic composition further comprising Penicillium emersonii GH61A polypeptide disclosed in WO 201 1/041397 (SEQ ID NO: 10 herein) and Aspergillus fumigatus beta-glucosidase (SEQ ID NO: 2 of WO 2005/047499) or SEQ ID NO: 35 8 herein, or a variant thereof, which variant has the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH1 disclosed as SEQ ID NO: 6 in WO201 1/057140 and SEQ ID NO: 6 herein and Aspergillus fumigatus CBH II disclosed as SEQ ID NO: 18 in WO 201 1/057140 and as SEQ ID NO: 7 herein.
- glucoamylase is derived from a strain of Trametes, such as Trametes cingulata; or Pachykytospora, such as Pachykytospora papyracea; or Leucopaxillus, such as Leucopaxillus giganteus.
- glucoamylase such as Trametes cingulata glucoamylase
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 12 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%,
- glucoamylase is from a strain of Aspergillus, preferably A. niger, A. awamori, or A. oryzae; or a strain of
- Trichoderma preferably Trichoderma reesei; or a strain of Talaromyces, preferably Talaromyces emersonii.
- glucoamylase such as Talaromyces emersonii glucoamylase
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g. , at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 1 1 herein.
- glucoamylase such as 20 Penicillium oxalicum glucoamylase
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 16 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g. , at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- glucoamylase is derived from a strain of Gloeophyllum, such as a strain of Gloeophyllum sepiarium, such as one shown in SEQ ID NO: 2 in WO 201 1/068803 or SEQ ID NO: 4 herein.
- glucoamylase such as Gloeophyllum sepiarium glucoamylase
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 4 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 35 70%, e.g. , at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 2 in WO 201 1/068803 or SEQ ID NO: 4 herein.
- glucoamylase is derived from a strain of Gloephyllum, such as one derived from a strain of Gloeophyllum trabeum, such as the one shown in SEQ ID NO: 18.
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 18 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- glucoamylase is Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein, preferably having one or more of the following substitutions: S95P, A121 P, especially S95P+ A21 P.
- 25 binding domain preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein, preferably one having one or more of the following substitutions: G128D, D143N, especially G128D+D143N.
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 17 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 17 herein.
- a pullulanase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 15 herein.
- the enzyme composition of paragraphs 42 or 43 comprising a pullulanase, such as a Bacillus deramificans pullulanase, a Talaromyces emersonii glucoamylase and/or
- Gloeophyllum sepiarium glucoamylase Gloeophyllum sepiarium glucoamylase.
- alpha-amylase is derived from a strain of the genus Rhizomucor, preferably a strain the Rhizomucor pusillus, such as the one shown in SEQ ID NO: 3 in WO 2013/006756, or the genus Meripilus, preferably a strain of Meripilus giganteus. 47.
- alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch- binding domain (SBD), such as the one shown in SEQ ID NO: 13 herein, or an alpha- amylase selected from the group consisting of:
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 13 herein.
- alpha-amylase is a variant of the alpha-amylase shown in SEQ ID NO: 13 having at least one of the following substitutions or combinations of substitutions: D165M; Y141W; Y141 R; K136F; K192R; P224A; P224R; S123H + Y141W; G20S + Y141W; A76G + Y141W; G128D + Y141W;
- alpha-amylase is derived from a Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch- binding domain (SBD), preferably disclosed as SEQ ID NO: 13 herein, preferably having one
- G128D, D143N, preferably G128D+D143N (using SEQ ID NO: 13 for numering).
- protease is a metallo protease derived from a strain of the genus Thermoascus, preferably a strain of Thermoascus aurantiacus, especially Thermoascus aurantiacus CGMCC No. 0670, such as the metallo protease disclosed as the mature part of SEQ ID NO: 2 disclosed in WO
- protease such as Thermoascus aurantiacus protease, is selected from the group consisting of:
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 3 herein.
- protease such as Pyrococcus furiosus protease
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 5 herein.
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 20 herein.
- composition of any of paragraphs 1 -59, wherein the composition comprises i) fungal glucoamylase;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising a GH61 polypeptide, beta-glucosidase CBH I and CBH II;
- Rhizomucor pusillus alpha-amylase or variant thereof
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei, further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta-glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II; optionally iv) protease from Thermoascus aurantiacus, or variant thereof.
- composition of any of paragraphs 1 -61 , wherein the composition comprises i) Trametes cingulata glucoamylase;
- Rhizomucor pusillus alpha-amylase or variant thereof
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei 35 cellulolytic composition further comprising Penicillium emersonii GH61A polypeptide, Aspergillus fumigatus beta-glucosidase with the following substitutions: F100D, S283G, N456E, F512Y, and optionally Aspergillus fumigatus CBH I and Aspergillus fumigatus CBH II;
- the enzyme composition of paragraph 63, wherein the trehalase is of fungal origin, such as derived from a strain of Trichoderma, such as Trichoderma reesei, such as the one shown in SEQ ID NO: 14 herein.
- trehalase such as 15 Trichoderma reesei trehalase
- a trehalase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- a process of producing a fermentation product from starch containing material comprising:
- saccharification and/or fermentation is done in the presence of the following enzymes: glucoamylase and alpha-amylase; and optionally protease.
- glucoamylase is Gloeophyllum glucoamylase, preferably Gloeophyllum trabeum glucoamylase.
- glucoamylase is the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 or a glucoamylase having at 35 least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18 herein.
- alpha-amylase is derived from Rhizomucor pusillus, preferably with an Aspergillus niger glucoamylase linker and starch- binding domain (SBD), preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO: 13 herein.
- SBD starch- binding domain
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 18 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 18 herein. 73.
- alpha-amylase is Rhizomucor pusillus alpha-amylase with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably one having at least one of the following substitutions or combinations of substitutions: D165M; Y141W; Y141 R; K136F; K192R; P224A; P224R; S123H+Y141W; G20S + Y141W; A76G + Y141W; G128D + Y141W; G128D + D143N; P219C + Y141W; N142D + D143N; Y141W + K192R; Y141W + D143N; Y141W + N383R; Y141W + P219C + A265C; Y141W + N142D + D143N; Y141W + K192R V410A; G128D + Y141W + D143N
- glucoamylase is the Gloeophyllum trabeum glucoamylase shown in SEQ ID NO: 18 herein having one of the following substitutions: S95P+A121 P and the alpha-amylase is is Rhizomucor pusillus alpha-amylase
- SBD Aspergillus niger glucoamylase linker and starch-binding domain
- glucoamylase comprising the mature polypeptide of SEQ ID NO: 17 herein;
- a glucoamylase comprising an amino acid sequence having at least 60%, at least 15 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 17 herein.
- glucoamylase is the Pycnoporus 20 sanguineus glucoamylase shown in SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 17 herein, and the alpha-amylase is the Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain
- SBD preferably the one disclosed as V039 in Table 5 in WO 2006/069290 or SEQ ID NO:
- an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 13 herein, preferably one having one or more of the following substitutions: G128D, D143N,
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to the mature polypeptide of SEQ ID NO: 19 herein.
- a protease comprising an amino acid sequence having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least
- protease optionally iv) protease.
- starch-containing material is plant material selected from the corn (maize), cobs, wheat, barley, rye, milo, sago, cassava, tapioca, sorghum, rice, peas, beans, sweet potatoes, or a mixture thereof, preferably corn.
- 20 25°C and 40°C preferably between 28°C and 36°C, such as between 28°C and 35°C, such as between 28°C and 34°C, such as around 32°C.
- cellulolytic enzyme composition is 10 present and/or added in an amount in the range from 0.1 -100 FPU per gram total solids (TS), preferably 0.5-50 FPU per gram TS, especially 1 -20 FPU per gram TS.
- TS FPU per gram total solids
- yeast such as yeast derived from a strain of the genus Saccharomyces, preferably a strain of Saccharomyces cerevisiae.
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof.
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or a variant thereof;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei;
- glucoamylase derived from Gloeophyllum trabeum shown in SEQ ID NO: 18 herein, 20 or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18 herein, preferably having at least one of the following substitutions: V59A; S95P; A121 P; T1 19W; S95P+A121 P; V59A+S95P; S95P+ T1 19W; V59A+S95P+A121 P; or S95P+T1 19W+A121 P, 25 especially S95P+A121 P (using SEQ ID NO: 18 for numbering);
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least
- SBD starch-binding domain
- SEQ ID NO: 18 30 98%, or at least 99% identity to SEQ ID NO: 18 herein, preferably one having at least one of the following substitutions or combinations of substitutions: D165M; Y141 W; Y141 R; K136F;
- glucoamylase derived from Gloeophyllum trabeum shown in SEQ ID NO: 18, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 18, preferably5 having at least one of the following substitutions: V59A; S95P; A121 P; T1 19W; S95P+A121 P; V59A+S95P; S95P+ T1 19W; V59A+S95P+A121 P; or S95P+T1 19W+A121 P, especially S95P+A121 P (using SEQ ID NO: 18 for numbering);
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), preferably one having at least one of0 the following substitutions or combinations of substitutions: D165M; Y141 W; Y141 R; K136F;
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei; preferably a cellulolytic enzyme composition derived from Trichoderma reesei further0 comprising Penicillium emersonii GH61A polypeptide disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, and Aspergillus fumigatus beta-glucosidase disclosed as SEQ ID NO: 2 in WO 2005/047499 or SEQ ID NO: 8 herein, or a variant thereof, preferably a variant having one of, preferably all of, the following substitutions: F100D, S283G, N456E, F512Y and optionally Aspergillus fumigatus Cel7A CBH1 disclosed5 as SEQ ID NO: 6 in WO201 1/057140 and SEQ ID NO: 6 herein and Aspergillus fumigatus CBH II disclosed as SEQ ID NO: 18 in WO 201
- a protease from Thermoascus aurantiacus or a variant thereof and/or Pyrococcus furiosus, or Meripilus giganteus.
- glucoamylase derived from Pycnoporus sanguineus shown in SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least5 98%, or at least 99% identity to SEQ ID NO: 17 herein;
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), or an alpha-amylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at0 least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to
- SEQ ID NO: 13 herein, preferably one having at least one of the following substitutions or combinations of substitutions: D165M; Y141W; Y141 R; K136F; K192R; P224A; P224R; S123H+Y141 W; G20S + Y141W; A76G + Y141W; G128D + Y141W; G128D + D143N; P219C + Y141W; N142D +5 D143N; Y141W + K192R; Y141W + D143N; Y141W + N383R; Y141W +
- cellulolytic enzyme composition derived from a strain of Trichoderma reesei; preferably a cellulolytic composition derived from Trichoderma reesei further comprising Penicillium emersonii GH61A polypeptide disclosed as SEQ ID NO: 2 in WO 201 1/041397 or SEQ ID NO: 10 herein, and Aspergillus fumigatus beta-glucosidase disclosed as SEQ ID5 NO: 2 in WO 2005/047499 or SEQ ID NO: 8 herein, or a beta-glucosidase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to SEQ ID NO: 8 herein, or a variant thereof, preferably a variant having
- a protease from Thermoascus aurantiacus or a variant thereof and/or Pyrococcus furiosus, or Meripilus giganteus.
- glucoamylase derived from Pycnoporus sanguineus shown in SEQ ID NO: 17 herein, or a glucoamylase having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least
- alpha-amylase derived from Rhizomucor pusillus with an Aspergillus niger glucoamylase linker and starch-binding domain (SBD), having the following substitutions G128D+D143N (using SEQ ID NO: 13 herein for numbering); iii) optionally cellulolytic enzyme composition derived from a strain of Trichoderma reesei;
- Meripilus giganteus protease 3 preferably the one shown in SEQ ID NO:
- protease having at least 60%, at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 91 %, at least 92%, at least
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CA2918685A CA2918685C (en) | 2013-08-30 | 2014-08-27 | Enzyme composition and uses thereof |
EP14839219.4A EP3039131A4 (en) | 2013-08-30 | 2014-08-27 | Enzyme composition and uses thereof |
CN201480042186.5A CN105431528A (en) | 2013-08-30 | 2014-08-27 | Enzyme composition and uses thereof |
US14/915,484 US10450551B2 (en) | 2013-08-30 | 2014-08-27 | Enzyme composition and uses thereof |
BR112016003339-6A BR112016003339B1 (en) | 2013-08-30 | 2014-08-27 | ENZYME COMPOSITION, AND, PROCESS OF PRODUCING A FERMENTATION PRODUCT FROM STARCH CONTAINING MATERIAL |
US16/567,167 US11155798B2 (en) | 2013-08-30 | 2019-09-11 | Enzyme composition and uses thereof |
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Cited By (9)
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US20170159090A1 (en) * | 2014-02-07 | 2017-06-08 | Novozymes A/S | Compositions for Producing Glucose Syrups |
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EP3039131A4 (en) | 2017-02-08 |
CN105431528A (en) | 2016-03-23 |
CA2918685A1 (en) | 2015-03-05 |
BR112016003339A2 (en) | 2017-11-21 |
US11155798B2 (en) | 2021-10-26 |
US20160201041A1 (en) | 2016-07-14 |
US20190390183A1 (en) | 2019-12-26 |
EP3039131A1 (en) | 2016-07-06 |
CA2918685C (en) | 2024-01-02 |
BR112016003339B1 (en) | 2023-02-14 |
US10450551B2 (en) | 2019-10-22 |
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