WO2014196703A1 - Detection reagent and detection kit for residual chlorine ion concentration, and detection method for residual chlorine ion concentration using same - Google Patents

Detection reagent and detection kit for residual chlorine ion concentration, and detection method for residual chlorine ion concentration using same Download PDF

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WO2014196703A1
WO2014196703A1 PCT/KR2013/010596 KR2013010596W WO2014196703A1 WO 2014196703 A1 WO2014196703 A1 WO 2014196703A1 KR 2013010596 W KR2013010596 W KR 2013010596W WO 2014196703 A1 WO2014196703 A1 WO 2014196703A1
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residual chlorine
chlorine ion
detection
reagent
ion concentration
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PCT/KR2013/010596
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French (fr)
Korean (ko)
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박종택
김현욱
정헌상
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서울시립대학교 산학협력단
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Publication of WO2014196703A1 publication Critical patent/WO2014196703A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/182—Water specific anions in water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water

Definitions

  • the present invention relates to a reagent for detecting residual chlorine ions, a detection kit, and a detection method, and more specifically, to a sample containing residual chlorine ions to be measured, a chlorine powder reagent and It relates to a method and a detection kit for calculating the residual chlorine ion concentration by adding this.
  • the residual chlorine concentration is measured by using the DPD method and the O-tololidine method, which is mainly performed by the O-tololidine method.
  • Residual chlorine in water is present in the form of chlorine, hypochlorous acid, hypochlorite ion and chloroamine, as shown in the reversible equilibrium form. Exists as.
  • a flat, colorless test tube with a stopper drawn at a specified level and labeled.
  • the combined residual chlorine concentration (mg / l) is obtained from the difference between the residual chlorine concentration and the free residual chlorine concentration (mg / l).
  • the residual chlorine measuring method has a lot of reagent preparation, a very complicated procedure, and the problem of the accuracy of the measurement varies greatly depending on the pH of the sample.
  • the present invention solves the above problems,
  • the present invention provides a detection reagent, a detection kit, and a detection method which can significantly exclude the influence of interference by other kinds of ions that may occur when measuring residual chlorine ion concentration.
  • the present invention is to provide a detection reagent, a detection kit, and a detection method capable of measuring residual chlorine ion concentration using colorimetric contrast as well as absorbance spectroscopy.
  • the present invention is to provide a detection reagent, a detection kit and a detection method capable of measuring the residual chlorine ion concentration that does not need to adjust the pH separately in the sample.
  • the present invention is a detection process that can measure the residual chlorine ion concentration, which solves the disadvantage that the measurement process is very complicated with a variety of reagents and complex procedures, and the cost is uneconomical and takes a long time to measure according to various reagents It is intended to provide a reagent, a detection kit and a detection method.
  • the present invention to solve the above problems and needs,
  • sodium phosphate is sodium phosphate dibasic anhydrous (Sodium phosphate dibasic anhydrous),
  • Ethylene diamine tetraacetic acid is ethylene diamine tetraacetic acid disodium salt dihydrate
  • Citric acid is Citric aicd trisodium salt hydrate
  • Potassium phosphate provides a residual chlorine ion detection reagent characterized by being Potasium phosphate monobasic.
  • the present invention also provides a residual chlorine ion concentration detection kit including the residual chlorine ion detection reagent.
  • a method for detecting residual chlorine ion concentration using the absorbance method or the colorimetric method is provided.
  • the residual chlorine measuring powder reagent of the present invention When the residual chlorine measuring powder reagent of the present invention is used, the effect of remarkably excluding the influence of interference by other kinds of ions that may occur when measuring residual chlorine ions is obtained.
  • the measurement using the residual chlorine powder reagent of the present invention does not require excessive measurement time and the effect can be performed very simply.
  • the phosphate-phosphorus concentration detection reagent of the present invention is simple to manufacture and economical.
  • 1 is a calibration curve for the standard solution.
  • the present invention provides a reagent, a detection kit, or a detection method capable of measuring the concentration of residual chlorine ions in a sample.
  • the present invention uses absorbance spectroscopy or colorimetric control as a method for detecting the concentration of chlorine ions in a sample, and provides a chlorine powder and a detection kit that meets and develops chlorine ions included in the sample. .
  • Residual chlorine powder reagent of the present invention (Sodium phosphate), N, N-Diethyl-p-phenylendiamine sulfate (N, N-Diethyl-p-phenylendiamine sulfate), ethylene diamine tetraacetic acid ( Consists of ethylene diamine tetraacetic acid, citric acid, and potassium phosphate.
  • the residual chlorine measurement powder reagent of the present invention is 100 to 200 parts by weight of N, diethyl-p-phenylendiamine sulfate (N, N-diethyl-p-phenylendiamine sulfate) in 100 parts by weight of sodium phosphate, 25 to 50 parts by weight of ethylene diamine tetraacetic acid, 25 to 50 parts by weight of citric acid, and 10 to 25 parts by weight of potassium phosphate are excellent in color development and detection Effect.
  • the sodium phosphate (Sodium phosphate) performs a function of removing the interference ions or factors in the measurement of residual chlorine.
  • Sodium phosphate (Sodium phosphate) of the present invention is more preferably used sodium phosphate dibasic anhydrous (Sodium phosphate dibasic anhydrous).
  • Such sodium phosphate dibeize anhydrous has a remarkably high effect of removing interference ions and factors.
  • N, N-Diethyl-p-phenylendiamine sulfate (N, N-Diethyl-p-phenylendiamine sulfate) of the present invention reacts with the residual chlorine in the sample and functions to develop color.
  • Ethylene diamine tetraacetic acid (Ethylene diamine tetraacetic acid) of the present invention performs the action of removing the interference ions and blockers and quickly promote the color development of the sample.
  • ethylene diamine tetraacetic acid is to maximize the promotion of color development .
  • Said citric acid (Citric acid) of the present invention is to act to buffer the pH of the sample.
  • the citric acid of the present invention exhibits the effect of sharpening the color of the sample.
  • Citric acid (citric acid) of the present invention more preferably using citric acid trisdium salt hydrate (citric aicd trisodium salt hydrate) is to achieve the effect of optimizing the color clarity more.
  • Potassium phosphate of the present invention has a function and function to control and buffer the pH of the sample, thereby increasing the clarity of color development of the sample.
  • Potassium phosphate may be used in combination with the residual chlorine contained in the sample by using the Potassium phosphate monobasic to maximize color clarity.
  • the present invention provides a residual chlorine measurement powder reagent consisting of the above-described configuration, and also provides a detection kit comprising the residual chlorine measurement powder reagent.
  • the present invention has a reaction time of at least 30 seconds after adding a predetermined amount of the residual chlorine measurement powder reagent to the sample.
  • the degree of color development becomes strong in proportion to the residual chlorine concentration in the sample water, and the residual chlorine of the sample is measured by measuring the degree of color development.
  • the present invention compares or compares the developed color of the sample to be measured and the level of color developed by adding the residual chlorine measurement reagent of the present invention to the standard solution for each residual chlorine ion concentration. It provides a method for measuring the concentration of.
  • the method of contrasting or comparing the degree of color development between the sample and the standard solution may be employed in various ways.
  • the concentration of residual chlorine ions may be calculated by using an absorbance method or colorimetric control.
  • the residual chlorine ion concentration detection method using the above-described residual chlorine measurement powder reagent of the present invention is as follows.
  • the present invention performs a process of generating a sample by reacting by mixing the residual chlorine measurement powder reagent to the sample.
  • the color reaction time is preferably 30 seconds or more as described above, it is appropriate to perform about 30 ⁇ 120 seconds.
  • the absorbance method used in the present invention means a conventional method of determining the concentration of the target component by measuring the degree of light absorption at a specific wavelength by changing the target component in the sample solution into a light absorbing material with a suitable reagent.
  • a method of measuring the concentration in the absorbance method there is a colorimetric method using the degree of color development, and a turbidity method using the degree of suspension of the sample solution.
  • Absorption spectroscopy makes a control solution corresponding to the sample liquid to measure the absorbance, and accordingly prepares a calibration curve and then measures the absorbance of the sample liquid and compares the concentration with the calibration curve.
  • both the colorimetric method and the colorimetric method can be used, and preferably, the colorimetric method has high accuracy.
  • a standard solution is prepared according to various concentrations of a desired component, and a standard colorimetric table is set by mixing the residual chlorine measurement reagent described above with respect to the standard solution.
  • the concentration of the sample solution is measured by comparing the color development degree obtained by mixing the residual chlorine measurement powder reagent with the sample and mixing with the standard colorimetric table.
  • the present invention can perform the absorbance measurement method or the colorimetric contrast measurement method described above using the residual chlorine measurement powder reagent or the detection kit including the same.
  • the colored sample was placed in a 1-inch-diameter round glass bottle, and the measurement wavelength was 530 nm.
  • the use of the residual chlorine ion concentration detection method using the residual chlorine measurement reagent according to the present invention has an effect of significantly reducing the influence of interference by other kinds of ions contained in the sample.

Abstract

The present invention relates to a detection reagent, detection kit and detection method for a residual chlorine concentration and, more particularly, to a residual chlorine detection powder reagent, and a method and a detection kit for calculating the residual chlorine concentration by adding same to a sample containing residual chlorine to be measured. The present invention provides the detection reagent for residual chlorine ion, consisting of sodium phosphate, N, N-Diethyl-p-phenylendiamine sulfate, ethylene diamine tetraacetic acid, citric acid and potassium phosphate. Also, provided is the detection reagent for the residual chlorine ion, wherein the sodium phosphate is sodium phosphate dibasic anhydrous, the ethylene diamine tetraacetic acid is ethylene diamine tetraacetic acid disodiumsalt dihydrate, the citric acid is citric aicd trisodium salt hydrate and the potassium phosphate is potassium phosphate monobasic. Furthermore, provided is a detection kit for residual chlorine ion concentration, comprising the detection reagent for the residual chlorine ion. In addition, provided is the method for detecting the residual chlorine ion concentration by using: a process of inducing a color development by adding a certain amount of the detection reagent to the sample comprising the residual chlorine ion to be measured and reacting same; and absorption spectrophotometry or colorimetry applying the degree of the induced color development.

Description

잔류염소 이온 농도 검출시약 및 검출키트 및 이를 이용한 잔류염소 이온 농도 검출방법Residual chlorine ion concentration detection reagent and detection kit and method for detecting residual chlorine ion concentration
본 발명은 잔류염소(Chlorine) 이온 농도 검출시약, 검출키트 및 검출방법에 관한 것으로, 더욱 구체적으로는 측정하고자 하는 잔류염소(Chlorine) 이온을 함유한 시료에 잔류염소측정 분말(Chlorine Powder) 시약 및 이를 첨가하여 잔류염소(Chlorine) 이온 농도를 산출하는 방법 및 검출키트에 관한 것이다.The present invention relates to a reagent for detecting residual chlorine ions, a detection kit, and a detection method, and more specifically, to a sample containing residual chlorine ions to be measured, a chlorine powder reagent and It relates to a method and a detection kit for calculating the residual chlorine ion concentration by adding this.
최근 들어 하천수, 호수, 폐수 중에 포함된 잔류염소, 칼륨, 질소, 인 등의 오염원에 대한 환경규제가 점점 강화됨에 따라 방류수 또는 하천수에 대하여 여러 형태의 수질측정이 행하여 지고 있다. 이러한 수질측정 시 요구되는 특성으로는 정밀성과 재현성을 보장해야 하는 기본적인 요구사항 이외에 신속하고 편리해야 하며, 환경친화적이어야 하고 경제성을 갖추어야 한다.Recently, as environmental regulations on pollutants such as residual chlorine, potassium, nitrogen, and phosphorus contained in river water, lake, and waste water have been tightened, various types of water quality measurements have been made on discharged water or river water. In addition to the basic requirements to ensure precision and reproducibility, the characteristics required for water quality measurement should be fast, convenient, environmentally friendly and economical.
통상적으로 잔류염소 농도를 측정하는 방법은 DPD법, O-톨리딘[Ortho-Tolidine]법을 이용하는데 주로 O-톨리딘[Ortho-Tolidine]법으로 시행하고 있다.In general, the residual chlorine concentration is measured by using the DPD method and the O-tololidine method, which is mainly performed by the O-tololidine method.
수중의 잔류염소는 가역평형식에 나타난 것처럼 염소, 차아염소산, 차 아염소산 이온 및 클로로아민 등의 형으로 존재하면서 pH 2이상에서는 주로 차아염소산 또는 그 이온으로 존재하고 pH 2이하에서는 주로 염소의 형으로 존재한다.Residual chlorine in water is present in the form of chlorine, hypochlorous acid, hypochlorite ion and chloroamine, as shown in the reversible equilibrium form. Exists as.
오르소 톨리딘 시험방법에서의 pH는 1.3이하이므로 그 염소(Cl2)가 오르소-톨리딘과 반응하여 발색한다. Since the pH of ortho tolidine test method is below 1.3, the chlorine (Cl2) reacts with ortho- tolidine and develops color.
결합형 유효염소도 오르소-톨리딘용액이 첨가되면 평형이 좌측으로 이동하고, 최종적으로는 염소를 유리하여 오르소-톨리딘과 반응하면서 발색에 약간의 시간이 걸리기 때문에 유리형 유효염소와 구별하는 것이 가능하다.The combined effective chlorine is also distinguished from free effective chlorine because the equilibrium shifts to the left when the ortho-tolidine solution is added, and finally takes chlorine to react with ortho-tolidine and takes some time to develop. It is possible to do
상기한 O-톨리딘[Ortho-Tolidine]법을 간단히 설명하면 다음과 같다.The above-described O-tolidine method [Ortho-Tolidine] method is briefly described as follows.
가. 시약제조end. Reagent Manufacturing
(1) O-톨리딘용액(1) O-Tolidine solution
O-톨리딘염산염 1.35g을 물 800에 녹이고 염산 150와 물을 넣어 1l로 한후 갈색병에 넣어 보존하며, 6개월이내에 사용한다.Dissolve 1.35 g of O-Tolidine hydrochloride in 800 water, add 150 hydrochloric acid and water to 1 l, and store in a brown bottle. Use within 6 months.
(2) 완충액(2) buffer
미리 110에서 건조하고 데시케이터에서 식힌 인산1수소칼륨 22.86g과 인산2수소칼륨 46.14g을 무탄산수에 녹여 1l로 한 후 수일간 두었다가 생성된 침전물을 제거하여 원액으로 하고, 이 원액 400에 무탄산수를 넣어 2l로 한다. 이 용액의 pH는 6.45이다.22.86 g of potassium monohydrogen phosphate and 46.14 g of potassium dihydrogen phosphate, which were previously dried at 110 and cooled in a desiccator, were dissolved in non-carbonated water to make 1 l, and then left for several days to remove the precipitates. Add 2 liters of sparkling water. The pH of this solution is 6.45.
(3) 잔류염소표준용액(3) Residual Chlorine Standard Solution
크롬산칼륨 4.65g과 중크롬산칼륨 1.55g을 완충액에 녹여 1l로 한다.4.65 g of potassium chromate and 1.55 g of potassium dichromate are dissolved in buffer to make 1 l.
(4) 잔류염소표준비색표(4) Standard Chlorine Color Chart
(가) 100ml의 비색관을 쓸 때에는 잔류염소표준용액 및 완충액을 <표 1>의 비율로 취하여 각각 비색관에 넣고 해당하는 잔류염소의 농도(mg/l)를 기재한다.(A) When 100 ml of colorimetric tubes are used, take the remaining chlorine standard solution and buffer solution in the ratio of <Table 1>, put them in the colorimetric tubes, and record the corresponding concentration of residual chlorine (mg / l).
(나) 100ml이외의 비색관 또는 비색병을 쓸 때에는 1mg/l이하의 잔류염소표준비색표를 <표 1>에 따라 조정하고, 1mg/l를 초과하는 잔류염소표준비색표는 사용하는 용기의 액층에 따라서 <표 2>의 해당하는 난에 따라 잔류염소표준용액을 취하고 완충액을 사용하여 100ml로 한 후 각각을 소정의 용기에 넣고 해당하는 잔류염소의 mg/l를 기재한 것을 잔류염소표준비색표로 한다.(B) When using a color tube or color bottle other than 100 ml, adjust the residual chlorine color standard below 1 mg / l according to <Table 1>, and the residual chlorine color standard exceeding 1 mg / l shall be According to the liquid layer, take the residual chlorine standard solution according to the corresponding column of <Table 2>, make it 100ml using the buffer solution, put each into a predetermined container and describe the mg / l of the corresponding residual chlorine. Make a table.
표 1
잔류염소 (mg/ℓ) 잔류염소표준용액(㎖) 완충액 (㎖) 잔류염소 (mg/ℓ) 잔류염소표준용액(㎖) 완충액 (㎖)
0.01 0.1 99.9 0.70 7.0 93.0
0.02 0.2 99.8 0.80 8.0 92.0
0.05 0.5 99.5 0.90 9.0 91.0
0.07 0.7 99.3 1.00 10.0 90.0
0.10 1.0 99.0 1.5 15.0 85.0
0.15 1.5 98.5 2.0 19.7 80.3
0.20 2.0 98.0 3.0 29.0 71.0
0.25 2.5 97.5 4.0 39.0 61.0
0.30 3.0 97.0 5.0 48.0 52.0
0.35 3.5 96.5 6.0 58.0 42.0
0.40 4.0 96.0 7.0 68.0 32.0
0.45 4.5 95.5 8.0 77.5 22.5
0.50 5.0 95.0 9.0 87.0 13.0
0.60 6.0 94.0 10.0 97.0 3.0
Table 1
Residual Chlorine (mg / ℓ) Residual Chlorine Standard Solution (ml) Buffer (ml) Residual Chlorine (mg / ℓ) Residual Chlorine Standard Solution (ml) Buffer (ml)
0.01 0.1 99.9 0.70 7.0 93.0
0.02 0.2 99.8 0.80 8.0 92.0
0.05 0.5 99.5 0.90 9.0 91.0
0.07 0.7 99.3 1.00 10.0 90.0
0.10 1.0 99.0 1.5 15.0 85.0
0.15 1.5 98.5 2.0 19.7 80.3
0.20 2.0 98.0 3.0 29.0 71.0
0.25 2.5 97.5 4.0 39.0 61.0
0.30 3.0 97.0 5.0 48.0 52.0
0.35 3.5 96.5 6.0 58.0 42.0
0.40 4.0 96.0 7.0 68.0 32.0
0.45 4.5 95.5 8.0 77.5 22.5
0.50 5.0 95.0 9.0 87.0 13.0
0.60 6.0 94.0 10.0 97.0 3.0
[잔류염소표준비색표(100ml 비색관용)][Residual Chlorine Standard Colorimetric Table (for 100ml colorimetric tube)]
표 2
용기의 액층별 잔류염소표준용액 (㎖) 잔류염소(mg/ℓ) 2.5～ 5㎝ 10㎝ 20㎝ 24～ 30㎝
1.0 10.0 10.0 10.0 10.0
1.5 15.0 15.0 15.0 15.0
2.0 19.5 19.5 19.7 20.0
3.0 27.0 27.5 29.0 30.0
4.0 34.5 35.0 39.0 40.0
5.0 42.0 43.0 48.0 50.0
6.0 49.0 51.0 58.0 60.0
7.0 56.5 59.0 68.0 70.0
8.0 64.0 67.0 77.5 80.0
9.0 72.0 75.5 87.0 90.
10.0 80.0 84.0 97.0 100.0
TABLE 2
Residual chlorine standard solution for each liquid layer in the container (ml) Residual chlorine (mg / ℓ) 2.5-5 cm 10 cm 20 cm 24 to 30 cm
1.0 10.0 10.0 10.0 10.0
1.5 15.0 15.0 15.0 15.0
2.0 19.5 19.5 19.7 20.0
3.0 27.0 27.5 29.0 30.0
4.0 34.5 35.0 39.0 40.0
5.0 42.0 43.0 48.0 50.0
6.0 49.0 51.0 58.0 60.0
7.0 56.5 59.0 68.0 70.0
8.0 64.0 67.0 77.5 80.0
9.0 72.0 75.5 87.0 90.
10.0 80.0 84.0 97.0 100.0
[잔류염소표준비색표(100ml외의 비색관용)][Residual Chlorine Standard Colorimetric Table (For Color Tubes Other than 100ml)]
(다) 이 잔류염소표준비색표는 어두운 곳에 보존하고 침전물이 생성되었을 때에는 다시 만든다.(C) This residual chlorine colorimetric table is stored in a dark place and regenerated when a precipitate is formed.
나. 기구I. Instrument
(1) 비색관(1) colorimetric tube
마개있는 밑이 평평한 무색시험관으로 일정용량의 높이에 표시선을 그은 것을 사용한다.A flat, colorless test tube with a stopper, drawn at a specified level and labeled.
다. 시험 및 잔류염소 측정All. Test and residual chlorine measurement
(1) 유리잔류염소(1) Free residual chlorine
O-톨리딘용액을 비색관용액(Vml)의 1/20에 상당하는 양을 취하여 비색관에 넣고 이에 검수를 비색관의 표시선까지 넣어 섞은 다음 즉시(약 5초이내) 잔류염소표준비색표와 비교하여 검수의 유리잔류염소농도(mg/l)를 구한다.Take the O-tolidine solution equal to 1/20 of the colorimetric tube solution (Vml), place it in the colorimetric tube, mix the test to the marking line of the colorimetric tube, and immediately (within 5 seconds) Compare and obtain the free residual chlorine concentration (mg / l) of the test.
(2) 잔류염소(2) residual chlorine
(1)의 액을 약 5분간 둔 후의 정색을 잔류염소표준비색표와 비교하여 검수의 잔류염소농도(mg/l)를 구한다.After leaving the solution in (1) for about 5 minutes, compare the color with the residual chlorine colorimetric table to determine the residual chlorine concentration (mg / l).
(3) 결합잔류염소(3) combined residual chlorine
잔류염소농도와 유리잔류염소농도(mg/l)와의 차이로부터 결합잔류염소농도(mg/l)를 구한다.The combined residual chlorine concentration (mg / l) is obtained from the difference between the residual chlorine concentration and the free residual chlorine concentration (mg / l).
상기한 바와 같은 잔류염소 측정 방법은 시약 준비가 많고 절차가 매우 복잡하며 시료의 PH에 따라 측정의 정확도가 많이 차이 나는 문제점이 지적되고 있다. As described above, the residual chlorine measuring method has a lot of reagent preparation, a very complicated procedure, and the problem of the accuracy of the measurement varies greatly depending on the pH of the sample.
본 발명은 상기와 같은 문제점을 해결하고,The present invention solves the above problems,
잔류염소 이온 농도 측정시에 발생할 수 있는 다른 종류의 이온에 의한 간섭의 영향을 현저히 배제할 수 있는 검출시약, 그 검출키트 및 검출방법을 제공하고자 한다.The present invention provides a detection reagent, a detection kit, and a detection method which can significantly exclude the influence of interference by other kinds of ions that may occur when measuring residual chlorine ion concentration.
또한 상기한 종래기술의 문제와 같이 과도한 측정 준비시간 및 측정과정을 요하지 않는 간단한 측정을 수행할 수 있는 잔류염소측정분말 검출시약, 검출키트 및 검출방법을 제공하고자 한다.In addition, to provide a residual chlorine measurement powder detection reagent, a detection kit and a detection method capable of performing a simple measurement that does not require excessive measurement preparation time and measurement process, as in the above-described problems of the prior art.
또한 본 발명은 흡광광도법 뿐만 아니라 비색표대조를 이용하여 잔류염소 이온 농도를 측정할 수 있는 검출시약, 검출키트 및 검출방법을 제공하고자 한다.In addition, the present invention is to provide a detection reagent, a detection kit, and a detection method capable of measuring residual chlorine ion concentration using colorimetric contrast as well as absorbance spectroscopy.
또한 본 발명은 시료에 별도의 PH 조절을 할 필요가 없는 잔류염소 이온 농도를 측정할 수 있는 검출시약, 검출키트 및 검출방법을 제공하고자 한다.In another aspect, the present invention is to provide a detection reagent, a detection kit and a detection method capable of measuring the residual chlorine ion concentration that does not need to adjust the pH separately in the sample.
또한 본 발명은 다양한 시약과 복잡한 절차로 측정과정이 매우 복잡하고 또한 다양한 시약을 사용함에 따라 그 비용면에서도 비경제적인 면 그리고 측정시간이 오래 걸리는 단점을 해결하는 잔류염소 이온 농도를 측정할 수 있는 검출시약, 검출키트 및 검출방법을 제공하고자 한다.In addition, the present invention is a detection process that can measure the residual chlorine ion concentration, which solves the disadvantage that the measurement process is very complicated with a variety of reagents and complex procedures, and the cost is uneconomical and takes a long time to measure according to various reagents It is intended to provide a reagent, a detection kit and a detection method.
본 발명은 상기한 문제점 및 요구를 해결하기 위하여,The present invention to solve the above problems and needs,
소디움 포스페이트(Sodium phosphate), 엔,엔 디에틸 피 페닐렌디아민 설페이트(N, N-Diethyl-p-phenylendiamine sulfate), 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid), 사이트릭 애시드(Citric acid), 포타시움 포스페이트(Potassium phosphate )로 구성된 잔류염소 이온 검출시약을 제공한다.Sodium phosphate, N-Diethyl-p-phenylendiamine sulfate (N, N-Diethyl-p-phenylendiamine sulfate), ethylene diamine tetraacetic acid, citric acid To provide a residual chlorine ion detection reagent consisting of, Potassium phosphate.
또한 상기한 소디움 포스페이트는 소디움 포스페이트 디베이식 안하이드로스(Sodium phosphate dibasic anhydrous)이고,In addition, the above-described sodium phosphate is sodium phosphate dibasic anhydrous (Sodium phosphate dibasic anhydrous),
에틸렌 디아민 테트라아세틱 애시드는 에틸렌 디아민 테트라아세틱 애시드 디소디움솔트 디하이드레이트(Ethylene diamine tetraacetic acid disodiumsalt dihydrate)이고,Ethylene diamine tetraacetic acid is ethylene diamine tetraacetic acid disodium salt dihydrate,
사이트릭 애시드(Citric acid)는 사이트릭 애시드 트리소디움 솔트 하이드레이트(Citric aicd trisodium salt hydrate)이고,Citric acid is Citric aicd trisodium salt hydrate,
포타시움 포스페이트(Potassium phosphate )는 포타시움 포스페이트 모노베이직(Potassium phosphate monobasic)인 것에 특징이 있는 잔류염소이온 검출시약을 제공한다.Potassium phosphate provides a residual chlorine ion detection reagent characterized by being Potasium phosphate monobasic.
또한 상기의 잔류염소이온 검출시약을 포함하여 구성한 잔류염소이온 농도 검출키트를 제공한다.The present invention also provides a residual chlorine ion concentration detection kit including the residual chlorine ion detection reagent.
또한 측정하고자 하는 잔류염소 이온이 포함된 시료에 상기의 검출시약을 일정량을 첨가하여 반응을 시켜 발색을 유도하는 과정, In addition, a process of inducing color reaction by adding a predetermined amount of the detection reagent to the sample containing the residual chlorine ions to be measured,
상기 유도된 발색 정도를 흡광광도법 또는 비색법을 이용하여 잔류염소이온 농도를 검출하는 방법을 제공한다.Provided is a method for detecting residual chlorine ion concentration using the absorbance method or the colorimetric method.
본 발명의 잔류염소측정분말 시약을 사용하는 경우 잔류염소이온 측정시에 발생할 수 있는 다른 종류의 이온에 의한 간섭의 영향을 현저히 배제할 수 있는 효과가 나타난다.When the residual chlorine measuring powder reagent of the present invention is used, the effect of remarkably excluding the influence of interference by other kinds of ions that may occur when measuring residual chlorine ions is obtained.
또한 본 발명의 잔류염소측정분말 시약을 사용하여 측정하는 경우 과도한 측정시간을 요하지 않고 그 절차도 매우 간단히 수행할 수 있는 효과가 나타난다.In addition, the measurement using the residual chlorine powder reagent of the present invention does not require excessive measurement time and the effect can be performed very simply.
또한 본 발명의 검출시약을 이용하여 잔류염소 이온 농도를 측정할 경우 기존의 측정방법과 대비하여 동등 이상의 정확도를 확보할 수 있는 효과를 나타낸다.In addition, when the residual chlorine ion concentration is measured using the detection reagent of the present invention, compared to the existing measurement method has the effect of ensuring the same or more accuracy.
또한 본 발명의 검출시약을 이용하여 잔류염소 이온 농도를 측정하는 경우 시료가 산성이나 염기성인 경우에도 별도의 PH 조절을 할 필요가 없는 효과가 창출된다.In addition, when the residual chlorine ion concentration is measured using the detection reagent of the present invention, even if the sample is acidic or basic, the effect of not having to adjust the pH is created.
더불어, 본 발명의 인산염-인 농도 검출시약은 제조가 간단하며 경제적인 특징이 있다.In addition, the phosphate-phosphorus concentration detection reagent of the present invention is simple to manufacture and economical.
도 1은 표준액에 대한 검량선. 1 is a calibration curve for the standard solution.
이하 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 시료 중에 잔류 염소 이온의 농도를 측정할 수 있는 시약, 검출 키트 또는 검출 방법을 제공한다.The present invention provides a reagent, a detection kit, or a detection method capable of measuring the concentration of residual chlorine ions in a sample.
본 발명은 시료의 염소 이온 농도를 검출하기 위한 방법으로 흡광광도법 또는 비색표 대조법을 이용하며, 시료에 포함된 염소 이온과 만나서 발색을 시키는 잔류염소측정분말 시약(chlorine Powder) 및 검출키트를 제공한다.The present invention uses absorbance spectroscopy or colorimetric control as a method for detecting the concentration of chlorine ions in a sample, and provides a chlorine powder and a detection kit that meets and develops chlorine ions included in the sample. .
본 발명의 잔류염소측정분말 시약(chlorine Powder)은 소디움 포스페이트(Sodium phosphate), 엔,엔 디에틸 피 페닐렌디아민 설페이트(N, N-Diethyl-p-phenylendiamine sulfate), 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid), 사이트릭 애시드(Citric acid), 포타시움 포스페이트(Potassium phosphate )로 구성되어 있다.Residual chlorine powder reagent of the present invention (Sodium phosphate), N, N-Diethyl-p-phenylendiamine sulfate (N, N-Diethyl-p-phenylendiamine sulfate), ethylene diamine tetraacetic acid ( Consists of ethylene diamine tetraacetic acid, citric acid, and potassium phosphate.
더욱 바람직하게는 본 발명의 잔류염소측정분말 시약은 나트륨 포스페이트(Sodium phosphate) 100 중량부에 엔,엔디에틸 피 페닐렌디아민 설페이트(N, N-Diethyl-p-phenylendiamine sulfate)100~200중량부, 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid) 25~50중량부, 사이트릭 애시드(Citric acid) 25~50중량부, 포타시움 포스페이트(Potassium phosphate )10~25 중량부 혼합된 것이 발색 및 검출에서 탁월한 효과를 나타낸다.More preferably, the residual chlorine measurement powder reagent of the present invention is 100 to 200 parts by weight of N, diethyl-p-phenylendiamine sulfate (N, N-diethyl-p-phenylendiamine sulfate) in 100 parts by weight of sodium phosphate, 25 to 50 parts by weight of ethylene diamine tetraacetic acid, 25 to 50 parts by weight of citric acid, and 10 to 25 parts by weight of potassium phosphate are excellent in color development and detection Effect.
본 발명은 상기한 소디움 포스페이트(Sodium phosphate)는 잔류염소 측정 시의 방해 이온이나 인자를 제거하는 기능을 수행한다.The present invention, the sodium phosphate (Sodium phosphate) performs a function of removing the interference ions or factors in the measurement of residual chlorine.
본 발명의 상기한 소디움 포스페이트(Sodium phosphate)는 더욱 바람직하게는 소디움 포스페이트 디베이식 안하이드로스(Sodium phosphate dibasic anhydrous)를 사용하는 것이 좋다.Sodium phosphate (Sodium phosphate) of the present invention is more preferably used sodium phosphate dibasic anhydrous (Sodium phosphate dibasic anhydrous).
이와 같은 소디움 포스페이트 디베이식 안하이드로스는 방해 이온이나 인자의 제거 능률이 현저히 높은 효과가 있다.Such sodium phosphate dibeize anhydrous has a remarkably high effect of removing interference ions and factors.
본 발명의 상기한 엔,엔디에틸 피 페닐렌디아민 설페이트(N, N-Diethyl-p-phenylendiamine sulfate)는 시료 중의 잔류염소와 반응하여 발색을 하는 기능과 작용을 한다.N, N-Diethyl-p-phenylendiamine sulfate (N, N-Diethyl-p-phenylendiamine sulfate) of the present invention reacts with the residual chlorine in the sample and functions to develop color.
본 발명의 상기한 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid)는 방해이온 및 방해인자를 제거하고 시료의 발색을 빠르게 촉진시키는 작용을 수행한다.Ethylene diamine tetraacetic acid (Ethylene diamine tetraacetic acid) of the present invention performs the action of removing the interference ions and blockers and quickly promote the color development of the sample.
더욱 바람직하게는 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid)는 에틸렌 디아민 테트라아세틱 애시드 디소디움솔트 디하이드레이트(Ethylene diamine tetraacetic acid disodiumsalt dihydrate)를 사용하는 것이 발색의 촉진이 최대화되는 작용을 하게 된다.More preferably, the use of ethylene diamine tetraacetic acid (Ethylene diamine tetraacetic acid disodiumsalt dihydrate) is to maximize the promotion of color development .
본 발명의 상기한 사이트릭 애시드(Citric acid)는 시료의 PH를 완충해 주는 작용을 하게 된다.Said citric acid (Citric acid) of the present invention is to act to buffer the pH of the sample.
따라서 시료가 중성 내지 약산일 경우 잔류염소와 반응하여 발색하는 것이 최적화되므로 본 발명의 사이트릭 애시드(Citric acid)는 시료의 발색을 선명하게 하는 효과를 나타낸다.Therefore, when the sample is neutral to weak acid, since the color development is optimized by reacting with the residual chlorine, the citric acid of the present invention exhibits the effect of sharpening the color of the sample.
본 발명의 상기한 사이트릭 애시드(Citric acid)는 더욱 바람직하게는 사이트릭 애시드 트리소디움 솔트 하이드레이트(Citric aicd trisodium salt hydrate)를 쓰는 것이 더욱 발색을 선명도를 최적화하게 되는 효과를 보게 된다.Citric acid (citric acid) of the present invention more preferably using citric acid trisdium salt hydrate (citric aicd trisodium salt hydrate) is to achieve the effect of optimizing the color clarity more.
본 발명의 상기한 포타시움 포스페이트(Potassium phosphate )는 시료의 PH 조절 및 완충을 하게 하는 기능과 작용이 있어 시료의 발색의 선명도를 높이는 효과를 나타낸다.Potassium phosphate of the present invention has a function and function to control and buffer the pH of the sample, thereby increasing the clarity of color development of the sample.
더욱 바람직하게는 포타시움 포스페이트(Potassium phosphate )는 포타시움 포스페이트 모노베이직(Potassium phosphate monobasic)을 사용하는 것이 상기한 작용에 의하여 시료에 포함된 잔류염소와 결합하여 발색하는 선명도를 최대화하게 된다.More preferably, Potassium phosphate may be used in combination with the residual chlorine contained in the sample by using the Potassium phosphate monobasic to maximize color clarity.
본 발명은 상기한 바와 같은 구성으로 이루어진 잔류염소측정분말 시약을 제공하며 또한 잔류염소측정분말 시약을 포함하는 검출키트를 제공하게 된다.The present invention provides a residual chlorine measurement powder reagent consisting of the above-described configuration, and also provides a detection kit comprising the residual chlorine measurement powder reagent.
본 발명은 상기한 잔류염소측정분말 시약을 시료에 일정량 첨가한 후 최소 30초의 반응시간을 갖는다.The present invention has a reaction time of at least 30 seconds after adding a predetermined amount of the residual chlorine measurement powder reagent to the sample.
상기 반응시간동안 잔류염소측정 분말(Chlorine Powder)과 만난 잔류염소(Chlorine)는 상호간의 결합작용을 하게 되는데, 잔류염소(Chlorine)에 따라 잔류염소측정 분말(Chlorine Powder)과 만난 시료수의 색깔이 변화하게 된다.During the reaction time, the residual chlorine powder (Chlorine Powder) and the remaining chlorine (Chlorine) have a mutual interaction, the color of the number of samples met with the residual chlorine powder (Chlorine Powder) Will change.
즉, 잔류염소측정 분말(Chlorine Powder)이 첨가된 시료수의 경우 시료수 내의 잔류염소(Chlorine)농도에 비례하여 발색 정도가 강하게 나타나게 되며 이 발색정도를 측정하여 시료의 잔류염소를 측정하게 된다.That is, in the case of the sample water to which the residual chlorine powder is added, the degree of color development becomes strong in proportion to the residual chlorine concentration in the sample water, and the residual chlorine of the sample is measured by measuring the degree of color development.
이와 같이 본 발명은 측정하고자 하는 시료의 발색된 정도와 각 잔류염소 이온 농도에 대한 표준용액에 대하여 상기한 본 발명의 잔류염소측정분말 시약을 투입하여 나타난 발색의 정도와 대조 또는 비교하여 진류염소 이온의 농도를 측정하는 방법을 제공한다. As described above, the present invention compares or compares the developed color of the sample to be measured and the level of color developed by adding the residual chlorine measurement reagent of the present invention to the standard solution for each residual chlorine ion concentration. It provides a method for measuring the concentration of.
상기한 시료와 표준액과의 발색 정도를 대조 또는 비교하는 방법은 다양하게 채용할 수 있으며, 바람직하게는 흡광광도법 또는 비색표 대조를 이용하여 잔류염소 이온의 농도를 산출할 수 있다.The method of contrasting or comparing the degree of color development between the sample and the standard solution may be employed in various ways. Preferably, the concentration of residual chlorine ions may be calculated by using an absorbance method or colorimetric control.
본 발명의 상기한 잔류염소측정분말 시약을 사용하여 잔류염소 이온 농도 검출 방법을 설명하면 다음과 같다.The residual chlorine ion concentration detection method using the above-described residual chlorine measurement powder reagent of the present invention is as follows.
본 발명은 시료에 잔류염소측정분말 시약을 혼합하여 반응시켜 시료를 발생하는 과정을 수행한다.The present invention performs a process of generating a sample by reacting by mixing the residual chlorine measurement powder reagent to the sample.
발색반응시간은 상기한 바와 같이 30초 이상으로 하는 것이 좋으며 30~120초 정도를 수행하는 것이 적절하다.The color reaction time is preferably 30 seconds or more as described above, it is appropriate to perform about 30 ~ 120 seconds.
잔류염소 이온의 여러 가지 농도의 표준액에 대한 대조액을 만들어 이에 대한 검량선 또는 기준치를 만들고, 시료액에 대한 흡광도를 측정하고 상기 검량선 또는 기준치와 비교하여 잔류염소 이온 농도를 검출하는 과정을 수행한다.A control solution is prepared for a standard solution of various concentrations of residual chlorine ions, and a calibration curve or reference value thereof is made, and the absorbance of the sample solution is measured, and the residual chlorine ion concentration is detected by comparing with the calibration curve or reference value.
또는 상기한 여러 가지 농도의 표준액에 대한 대조액의 기준치와 시료액의 발색 정도를 비교하여 잔류염소 이온농도를 검출하는 비색 대비표 등의 방법을 수행한다.Alternatively, a method such as a color contrast table for detecting residual chlorine ion concentration is performed by comparing the reference value of the control solution with respect to the standard solution of various concentrations and the color development of the sample solution.
상기의 과정을 통하여 잔류염소 이온농도를 검출하는 방법을 제공한다.It provides a method for detecting the residual chlorine ion concentration through the above process.
본 발명에서 사용하는 흡광광도법은 시료 용액 중의 목적 성분을 적당한 시약으로 광흡수 물질로 변화시켜 특정 파장에서 광흡수 정도를 측정하여 목적 성분의 농도를 구하는 통상의 방법을 의미한다.The absorbance method used in the present invention means a conventional method of determining the concentration of the target component by measuring the degree of light absorption at a specific wavelength by changing the target component in the sample solution into a light absorbing material with a suitable reagent.
본 발명의 흡광광도법은 람버트 비어 법칙을 적용하여 목적 성분의 농도를 구하는 것으로 통상의 흡광광도 분석장치는 통상 광원부, 파장선택부, 시료부, 측광부 등으로 이루어져 있다.The absorbance method of the present invention obtains the concentration of a target component by applying Lambert Beer's law. A typical absorbance analyzer includes a light source unit, a wavelength selection unit, a sample unit, and a photometric unit.
흡광광도법에서 농도를 측정하는 방법으로는 색의 발색정도를 이용하는 비색법, 시료 용액의 현탁정도를 이용하는 비탁법 등이 있다. 흡광광도법은 시료액에 대응하는 대조액을 만들어 흡광도를 측정하고 이에 따라 검량선을 작성한 후 시료액의 흡광광도를 측정하여 상기 검량선과 비교하여 농도를 측정하게 된다.As a method of measuring the concentration in the absorbance method, there is a colorimetric method using the degree of color development, and a turbidity method using the degree of suspension of the sample solution. Absorption spectroscopy makes a control solution corresponding to the sample liquid to measure the absorbance, and accordingly prepares a calibration curve and then measures the absorbance of the sample liquid and compares the concentration with the calibration curve.
본 발명에서는 상기 비색법, 비탁법 모두 다 사용이 가능하며 바람직하게는 비색법으로 하는 것이 정확도가 높다. In the present invention, both the colorimetric method and the colorimetric method can be used, and preferably, the colorimetric method has high accuracy.
또한 비색표 대조법은 다음과 같다.In addition, the colorimetric contrast method is as follows.
상기한 바와 같이 목적하는 성분을 여러 가지 농도에 따라 표준액을 만들고 이 표준액에 대하여 상기한 잔류염소측정분말 시약을 혼합하여 기준 비색표를 설정한다. As described above, a standard solution is prepared according to various concentrations of a desired component, and a standard colorimetric table is set by mixing the residual chlorine measurement reagent described above with respect to the standard solution.
그리고 시료에 상기한 잔류염소측정분말 시약을 혼합하여 혼화 반응시킨 나온 발색정도를 상기 기준 비색표와 비교하여 시료액의 농도를 측정한다.Then, the concentration of the sample solution is measured by comparing the color development degree obtained by mixing the residual chlorine measurement powder reagent with the sample and mixing with the standard colorimetric table.
이와 같이 본 발명은 잔류염소측정분말 시약 또는 이를 포함한 검출키트를 이용하여 상기한 측정 방법인 흡광광도 측정방법 또는 비색표 대비측정 방법을 수행할 수 있게 된다.As described above, the present invention can perform the absorbance measurement method or the colorimetric contrast measurement method described above using the residual chlorine measurement powder reagent or the detection kit including the same.
이하 본 발명에 따른 실시예를 설명한다. 다만 본 발명의 실시예는 본 발명의 기술적 내용을 제한하거나 권리범위를 축소하는 것은 아니다.Hereinafter will be described an embodiment according to the present invention. However, the embodiment of the present invention does not limit the technical content of the present invention or reduce the scope of rights.
<실시예><Example>
1. 아래와 같이 본 발명의 잔류염소측정분말 시약을 제조한다.1. Prepare the residual chlorine measurement powder reagent of the present invention as follows.
<시약1> <Reagent 1>
Synonyms: Sodium phosphate dibasic anhydrous, 30g Synonyms: Sodium phosphate dibasic anhydrous, 30 g
CAS No.: 7758-79-4 CAS No .: 7758-79-4
<시약2><Reagent 2>
Synonyms: N. N-Diethyl-p-phenylendiamine sulfate, 45g Synonyms: N. N-Diethyl-p-phenylendiamine sulfate, 45 g
CAS No.: 6283-63-2 CAS No .: 6283-63-2
<시약3><Reagent 3>
Synonyms: Ethylene diamine tetraacetic acid disodiumsalt dihydrate, 10g Synonyms: Ethylene diamine tetraacetic acid disodiumsalt dihydrate, 10 g
CAS No.: 6384-92-6 CAS No .: 6384-92-6
<시약4><Reagent 4>
Synonyms: Citric aicd trisodium salt hydrate, 10g Synonyms: Citric aicd trisodium salt hydrate, 10 g
CAS No.: 114456-61-0 CAS No .: 114456-61-0
<시약5><Reagent 5>
Synonyms: Potassium phosphate monobasic, 5g Synonyms: Potassium phosphate monobasic, 5 g
CAS No.: 7778-77-0 CAS No .: 7778-77-0
을 혼합하여 분말형의 잔류염소측정분말 시약을 제조한다.To prepare a powdered residual chlorine powder reagent.
2. 잔류염소 이온 농도 검출과정2. Detection of residual chlorine ion concentration
(1) 흡광광도법 이용(1) Absorption Spectrophotometry
1) 상기한 잔류염소측정분말 시약을 시료 10ml에 100mg을 첨가하여 30초 이상 유지하여 발색을 유도한다.1) Add 100 mg of the above residual chlorine measuring reagent to 10 ml of the sample and hold for 30 seconds or more to induce color development.
2) 상기 발색된 시료를 측정용기 직경 1인치 원형 유리병에 넣고 측정파장은 530nm를 이용하였다.2) The colored sample was placed in a 1-inch-diameter round glass bottle, and the measurement wavelength was 530 nm.
3) 표준액에 대한 검량선을 작성한다.3) Prepare calibration curve for standard solution.
표준액에 대한 대조액 검량선은 도 1에 나타난 바와 같다.The control calibration curve for the standard solution is shown in FIG. 1.
상기한 대조액 검량선과 시료에 대한 흡광광도를 비교하여 정확한 잔류염소 이온 농도를 측정하게 된다.Accurate residual chlorine ion concentration is measured by comparing the absorbance of the sample with the control calibration curve.
(2) 비색표 대비법(2) colorimetric contrast method
상기한 바와 같이 표준액에 대한 비색표를 만들어 상기한 발색이 유발된 시료에 대한 발색 정도를 비교하여 잔류염소 이온 농도를 산출한다.As described above, a colorimetric table for the standard solution is prepared, and the residual chlorine ion concentration is calculated by comparing the degree of color development for the sample in which the color development is induced.
본 발명은 아래의 [표 3]에서 보는 바와 같이 이온의 간섭의 영향을 배제할 수 있는 높은 한계치를 나타내고 있다.As shown in Table 3 below, the present invention exhibits a high limit that can exclude the effect of interference of ions.
따라서 본 발명에 따른 잔류염소측정분말 시약을 이용한 잔류염소 이온 농도 검출방법을 이용하는 경우 시료 중에 포함된 다른 종류의 이온에 의한 간섭의 영향을 현저히 감소시키는 효과가 있음을 알 수 있다.Therefore, it can be seen that the use of the residual chlorine ion concentration detection method using the residual chlorine measurement reagent according to the present invention has an effect of significantly reducing the influence of interference by other kinds of ions contained in the sample.
표 3
방해물질 한계치(mg/l)
Acidity 150mg/l as CaCO3
Alkalinity 250mg/l as CaCO3
Bromine All levels
Hardness 1000mg/l as CaCO3
Iodine All levels
Ozone All levels
TABLE 3
Interference Limit value (mg / l)
Acidity 150mg / l as CaCO 3
Alkalinity 250 mg / l as CaCO 3
Bromine All levels
Hardness 1000mg / l as CaCO 3
Iodine All levels
Ozone All levels
상기한 바처럼 본 발명을 이용하여 잔류염소(Chlorine) 이온 농도를 측정할 경우 기존의 방법대비 동등 이상의 정확도를 확보할 수 있을 뿐만 아니라, 측정시간에 있어서도 잔류염소(Chlorine) 이온과 발색시약의 반응시간을 획기적으로 단축하는 효과를 창출하게 된다.As described above, when measuring the residual chlorine ion concentration using the present invention, not only can the accuracy be more than equivalent to that of the conventional method, but also the reaction of the residual chlorine ion and the coloring reagent in the measurement time. It will create a time-saving effect.
상기한 바와 같이 본 발명은 본 발명의 특징인 잔류염소측정분말 시약 및 이를 포함한 검출키트 및 이를 이용한 잔류염소 이온 농도 검출방법을 제공하게 된다.As described above, the present invention provides a residual chlorine measurement powder reagent, a detection kit including the same, and a method for detecting residual chlorine ion concentration using the same.
본 발명은 수질환경기본법과 관련된 정수 및 하폐수처리사업, 공해방지업, 교육사업 등에 널리 사용가능하다.The present invention can be widely used in water purification and sewage water treatment business, pollution prevention business, education business, etc. related to the Framework Act on Water Environment.
또한 본 발명은 공해방지모니터링업, 국가 및 지방정부의 환경정책관련업 등에 널리 이용가능하다.In addition, the present invention can be widely used for pollution prevention monitoring industry, environmental policy related business of national and local government.
이와 더불어, 수질분석약품사업, 수질분석약품제조업, 환경감리업, 환경모델링업, 환경영향평가업 및 교육용 자료 제공업 등에 매우 유용한 발명이다.In addition, it is a very useful invention for water quality analysis chemicals business, water quality analysis chemical manufacturing industry, environmental supervision business, environmental modeling business, environmental impact assessment business and educational data providing business.

Claims (4)

  1. 소디움 포스페이트(Sodium phosphate), 엔,엔 디에틸 피 페닐렌디아민 설페이트(N, N-Diethyl-p-phenylendiamine sulfate), 에틸렌 디아민 테트라아세틱 애시드(Ethylene diamine tetraacetic acid), 사이트릭 애시드(Citric acid), 포타시움 포스페이트(Potassium phosphate )로 구성된 잔류염소 이온 검출시약.Sodium Phosphate, N-Diethyl-p-phenylendiamine Sulfate, Ethylene diamine tetraacetic acid, Citric acid Residual chlorine ion detection reagent consisting of, Potassium phosphate.
  2. 제1항에 있어서,The method of claim 1,
    소디움 포스페이트는 소디움 포스페이트 디베이식 안하이드로스(Sodium phosphate dibasic anhydrous)이고,Sodium phosphate is sodium phosphate dibasic anhydrous,
    에틸렌 디아민 테트라아세틱 애시드는 에틸렌 디아민 테트라아세틱 애시드 디소디움솔트 디하이드레이트(Ethylene diamine tetraacetic acid disodiumsalt dihydrate)이고,Ethylene diamine tetraacetic acid is ethylene diamine tetraacetic acid disodium salt dihydrate,
    사이트릭 애시드(Citric acid)는 사이트릭 애시드 트리소디움 솔트 하이드레이트(Citric aicd trisodium salt hydrate)이고,Citric acid is Citric aicd trisodium salt hydrate,
    포타시움 포스페이트(Potassium phosphate )는 포타시움 포스페이트 모노베이직(Potassium phosphate monobasic)인 것에 특징이 있는 잔류염소이온 검출시약.Potassium phosphate (Potassium phosphate) is a residual chlorine ion detection reagent characterized in that Potassium phosphate monobasic.
  3. 제1항 또는 제2항의 잔류염소이온 검출시약을 포함하여 구성한 잔류염소이온 농도 검출키트.A residual chlorine ion concentration detection kit comprising the residual chlorine ion detection reagent according to claim 1.
  4. 측정하고자 하는 잔류염소 이온이 포함된시료에 제1항 내지 제2항의 검출시약을 일정량을 첨가하여 반응을 시켜 발색을 유도하는 과정, A process of inducing color reaction by adding a predetermined amount of the detection reagent of claim 1 to a sample containing residual chlorine ions to be measured,
    상기 유도된 발색 정도를 흡광광도법 또는 비색법을 이용하여 잔류염소이온 농도를 검출하는 방법.The method of detecting residual chlorine ion concentration using the absorbance or colorimetric method of the induced color development.
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