WO2014195400A1 - Kinase inhibitors - Google Patents

Kinase inhibitors Download PDF

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Publication number
WO2014195400A1
WO2014195400A1 PCT/EP2014/061677 EP2014061677W WO2014195400A1 WO 2014195400 A1 WO2014195400 A1 WO 2014195400A1 EP 2014061677 W EP2014061677 W EP 2014061677W WO 2014195400 A1 WO2014195400 A1 WO 2014195400A1
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Prior art keywords
alkyl
group
cycloalkyl
cycloalkylene
alkylene
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PCT/EP2014/061677
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French (fr)
Inventor
Chi-Kit Woo
Lilian Alcaraz
Carmelida CAPALDI
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Chiesi Farmaceutici S.P.A.
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Application filed by Chiesi Farmaceutici S.P.A. filed Critical Chiesi Farmaceutici S.P.A.
Priority to BR112015029462A priority Critical patent/BR112015029462A8/en
Priority to EP14733543.4A priority patent/EP3004098B1/en
Priority to CN201480032036.6A priority patent/CN105308046A/en
Priority to CA2914449A priority patent/CA2914449A1/en
Priority to RU2015151834A priority patent/RU2015151834A/en
Priority to KR1020157033448A priority patent/KR20160015221A/en
Publication of WO2014195400A1 publication Critical patent/WO2014195400A1/en
Priority to HK16107172.7A priority patent/HK1219104A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/10—Spiro-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems

Definitions

  • This invention relates to compounds and compositions that are p38 MAPK inhibitors, useful as anti-inflammatory agents in the treatment of, inter alia, diseases of the respiratory tract.
  • Mitogen activated protein kinases constitute a family of proline- directed serine/threonine kinases that activate their substrates by dual phosphorylation.
  • the p38 kinases which are also known as cytokine suppressive anti-inflammatory drug binding proteins (CSBP), stress activated protein kinases (SAPK) and RK, are responsible for phosphorylating (Stein et al., Ann. Rep.
  • TNF a tumor necrosis factor alpha
  • IL interleukin-
  • COX-2 cyclooxygenase-2
  • IL-1 and TNFa are also known to stimulate the production of other proinflammatory cytokines such as IL-6 and IL- 8.
  • IL-1 and TNFa are biological substances produced by a variety of cells, such as monocytes or macrophages.
  • IL-1 has been demonstrated to mediate a variety of biological activities thought to be important in immunoregulation and other physiological conditions such as inflammation (e.g. Dinarello et al., Rev. Infect. Disease, 1984, 6, 51).
  • Excessive or unregulated TNF production has been implicated in mediating or exacerbating a number of diseases, and it is believed that TNF can cause or contribute to the effects of inflammation in general.
  • IL-8 is a chemotactic factor produced by several cell types including mononuclear cells, fibroblasts, endothelial cells, and keratinocytes.
  • IL-8 stimulates a number of functions in vitro. It has been shown to have chemoattractant properties for neutrophils, T-lymphocytes and basophils. Increase in IL-8 production is also responsible for chemotaxis of neutrophils into the inflammatory site in vivo.
  • Inhibition of signal transduction via p38 which in addition to IL-1 , TNF and IL-8 described above is also required for the synthesis and/or action of several additional pro-inflammatory proteins (e.g., IL-6, GM-CSF, COX-2, collagenase and stromelysin), is expected to be a highly effective mechanism for regulating the excessive and destructive activation of the immune system. This expectation is supported by the potent and diverse anti- inflammatory activities described for p38 kinase inhibitors (Badger et al , J. Pharm. Exp. Thera., 1996, 279, 1453 -1461 ; Griswold et al, Pharmacol. Comm. ,1996, 7, 323-229).
  • p38 kinase inhibitors have been described as potential agents for treating rheumatoid arthritis.
  • Stress stimuli including tobacco smoke, infections or oxidative products
  • Inhibitors of p38 have been shown to inhibit LPS and ovalbumin induced airway TNF-a, IL- ⁇ ⁇ , IL-6, IL-4, IL-5 and IL-13 (Haddad et al, Br. J. Pharmacol, 2001 , 132 (8), 1715-1724; Underwood et al, Am. J.
  • COPD chronic obstructive pulmonary disease
  • asthma chronic or acute broncho constriction
  • bronchitis acute lung injury and bronchiectasis
  • pulmonary artery hypertension tuberculosis
  • tuberculosis lung cancer
  • inflammation generally (e.g.
  • inflammatory bowel disease arthritis, neuroinflammation, pain, fever, fibrotic diseases, pulmonary disorders and diseases (e.g., hyperoxic alveolar injury), cardiovascular diseases, post -ischemic reperfusion injury and congestive heart failure, cardiomyopathy, stroke, ischemia, reperfusion injury, renal reperfusion injury, brain edema, neurotrauma and brain trauma, neurodegenerative disorders, central nervous system disorders, liver disease and nephritis, gastrointestinal conditions, ulcerative diseases, Crohn's disease, ophthalmic diseases, ophthalmo logical conditions, glaucoma, acute injury to the eye tissue and ocular traumas, diabetes, diabetic nephropathy, skin-related conditions, myalgias due to infection, influenza, endotoxic shock, toxic shock syndrome, autoimmune disease, graft rejection, bone resorption diseases, multiple sclerosis, psoriasis, eczema, disorders of the female reproductive system, pathological (but non -malignant) conditions, such
  • TNF chronic release of active TNF can cause cachexia and anorexia, and TNF can be lethal.
  • TNF has also been implicated in infectious diseases. These include, for example, malaria, mycobacterial infection and meningitis.
  • viral infections such as HIV, influenza virus, and herpes virus, including herpes simplex virus type-1 (HSV-1), herpes simplex virus type-2 (HSV-2), cytomegalovirus (CMV), varicella-zoster virus (VZV), Epstein-Barr virus, human herpes virus-6 (HHV-6), human herpesvirus-7 (HHV7), human herpesvirus-8 (HHV-8), pseudorabies and rhino tracheitis, among others.
  • HSV-1 herpes simplex virus type-1
  • HSV-2 herpes simplex virus type-2
  • CMV cytomegalovirus
  • VZV varicella-zoster virus
  • Epstein-Barr virus Epstein-Barr virus
  • human herpes virus-6 HHV-6
  • P38 kinase inhibitors have been reviewed by G. J. Hanson (Expert Opinions on Therapeutic Patents, 1997, 7, 729-733) J Hynes et al. (Current Topics in Medicinal Chemistry, 2005, 5, 967-985), C. Dominguez et al (Expert Opinions on Therapeutics Patents, 2005, 15, 801-816) and L. H. Pettus & R. P. Wurtz (Current Topics in Medicinal Chemistry, 2008, 8, 1452-1467).
  • P38 kinase inhibitors containing a triazolopyridine motif are known in the art, for example WO07/091 152, WO04/072072, WO06/018727.
  • A represents an optionally substituted divalent arylene radical, an heteroarylene radical, a (C 5 -C 6 ) divalent cycloalkylene radical having 5 or 6 ring atoms or a pyperidinylene radical.
  • the compounds are said to be useful in as anti- inflammatory agents in the treatment of diseases of the respiratory tract.
  • the object of the present invention is to identify novel and potent p38 mitogen activated protein kinase inhibitors which are useful in the treatment of inflammatory and obstructive diseases of the respiratory tract. It is another object of the present invention, to identify novel potent p38 mitogen activated protein kinase inhibitors which show an appropriate developability profile on inhalatory administration to effectively treat respiratory obstructive or inflammatory diseases. It is to be understood that such profile may be achieved in a number of different ways by modulation of specific properties; by way of example, it could be achieved by administration of a low effective dose of the drug thus limiting side effects or via a long duration of action in the lungs which may reduce the frequency of administration.
  • the compounds of the present invention are inhibitors of p38 mitogen activated protein kinase ("p38 MAPK”, “p38 kinase” or “p38”), including p38a kinase, and are inhibitors of cytokine and chemokine production including TNFa and IL-8 production. They have a number of therapeutic applications, in the treatment of inflammatory diseases, particularly allergic and non-allergic airways diseases, more particularly obstructive or inflammatory airways diseases such as chronic obstructive pulmonary disease ("COPD”) and asthma. They are therefore particularly suited for pulmonary delivery, by inhalation by nose or mouth.
  • inflammatory diseases particularly allergic and non-allergic airways diseases, more particularly obstructive or inflammatory airways diseases such as chronic obstructive pulmonary disease ("COPD”) and asthma.
  • COPD chronic obstructive pulmonary disease
  • COPD chronic obstructive pulmonary disease
  • W is a heteroatom selected from N or O, wherein N is substituted with -H, Ci- C 6 alkyl or C3-C5 cycloalkyl;
  • Y is selected in the group consisting of: a group -S(0) p - wherein p is 0, 1 or 2; a group -0(CR 3 R 4 ) n -; a group -(CR 5 R 6 ) admir-; a group -NR 7 -; a group -OC(O)-; a group -OC(0)NH-; and a group -OC(0)0-;
  • n 0, 1 , 2 or 3;
  • R 3 , R 4 , R 5 and R 6 are each independently -H, fluorine or Ci-C 6 alkyl, or, respectively, R 3 and R 4 , or R 5 and R 6 may form together with the carbon atom to which they are attached a saturated 3-6 membered carbocyclic monocyclic ring optionally substituted by a group Ci-C 6 alkyl, hydroxyl or halo;
  • R 7 is -H, Ci-C 6 alkyl, or C 3 -C 7 cycloalkyl wherein such Ci-C 6 alkyl or C 3 -C 7 cycloalkyl are optionally substituted by a group C 1 -C 3 alkyl, C 3 -C6 cycloalkyl, hydroxyl, -CN or halo;
  • R 1 is a group (Ila)
  • X 2 , X 3 , X 4 and X s are each independently a carbon atom, a nitrogen atom, a group -(CH)- or a group -NH-; such that each combination thereof forms an aromatic ring system;
  • R 11 is linked to X 4 and is selected from a group consisting of:
  • C 7 heterocycloalkyl)(C 3 -C 6 cycloalkyl) and (C 3 -C 7 heterocycloalkyl)carbonyl wherein any of such Ci-C 6 alkyl, C 3 -C 6 cycloalkyl, -(Ci-Cealkylene)- -(C 2 - C 6 alkylene)-, -(C 3 -C 7 cycloalkylene)-, -(C 3 -C 7 heterocycloalkyl) and (C 5 - C 7 heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R 25 which are independently selected in the list consisting of: Ci-C 6 alkyl, (Ci-C 3 ) haloalkyl, (Ci-C4)hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxy 1 and halo; or
  • R 11 is linked to X 4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is optionally substituted by one or more group(s) independently selected from: halo, OR D , -CN, -S0 2 R D , Ci-C 6 alkyl which is substituted by one or more groups -CN; C 3 -C 6 cycloalkyl which is substituted by a group selected from: -CN, -OR c , -SR C or halo ; -N(R C )(C 2 -C 6 alkylene)-NR A R B , -N(R c )(C 3 -C 7 cycloalkylene)-NR A R B , -(Ci- C 6 alkylene)-NR A R B , -(C 3 -C 7 cycloalkylene)-NR A R B , -0-(C 3
  • R A and R B are at each occurrence independently -H, Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, -OR D , -CN, -NR A R B' or halo; alternatively, R A and R B , may form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more groups independently selected from -OR D , -CN, -NR A R B' , halo, Ci-C 6 alkyl, -(C 3 -C 7 ) cycloalkyl-NR A R B' , -(Ci-C 5 alkylene)-NR A' R B' , -
  • Ci-C 6 alkyl, C 2 -C 5 alkyl and C 3 -C 7 cycloalkyl portions being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, -OR D , -CN, -NR A R B or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C 3 - C 6 cycloalkyl, wherein any of such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl may be optionally substituted by a group Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, -OR D , -CN, or halo; or R A and R B may be linked to one carbon atom of the -(C)
  • R c may be linked to one carbon atom of the -(C 2 -C 6 alkylene)- or -(C3-Cycycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
  • R D is at each occurrence independently -H, -CH 3 , -C 2 H 5 or (Ci- C4)hydroxyalkyl;
  • R A and R B are at each occurrence independently -H, Ci-C 6 alkyl or C3-C7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C7 cycloalkyl being optionally substituted by a group C 1 -C 3 alkyl, C 3 -C 7 cycloalkyl, -OR D , -CN or halo; alternatively, R A and R B , may form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -OR D , -CN, halo, Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group C 1 -C 3 alkyl, C 3 -C 7 cycloalkyl, -OR D , -
  • R D is at each occurrence independently -H, -CH 3 or -C 2 H 5 ;
  • R c is at each occurrence independently -H, Ci-C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 6 cycloalkyl being optionally substituted by a group C 1 -C 3 alkyl, -OR D' , -CN or halo;
  • R 13 are independently -H, Ci-C 6 alkyl or halo;
  • A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms; said cycloalkylene ring being attached to W and Y, and optionally fused to a phenyl ring or to a monocyclic heteroaryl ring having 5 or 6 ring atoms, such phenyl or heteroaryl ring being optionally substituted by one or two groups R 24 ;
  • R 24 is at each occurrence independently selected from the group consisting of: Ci-Ce alkyl, halo and CN;
  • R 2 is a radical of formula
  • R 17 is a group of general formula (IV):
  • R 19 is selected in a group consisting of: -H, -CH 3 , CN and OH;
  • R 20 is selected in the group consisting of: -H, -F, -CH 3 , -C 2 H 5 , -CH 2 OH, -CH 2 OMe, -CF 2 CF 3 , -CH 2 SCH 3 , -SCH 3 and -SC 2 H 5 ;
  • R 21 is -H, -CH 3 or -C 2 H 5 ;
  • R 18 is absent or is selected in the group consisting of aryl, heteroaryl, -(Ci-C 6 alkyl), -(C 3 -C 7 cycloalkyl), -(C 3 -Cyheterocycloalkyl), (C 5 -Cvheterocycloalkyl)-(Ci-C 6 alkyl) and (C5-Cyheterocycloalkyl)-(C 3 -C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C 6 alkyl), -(C 3 -Cycycloalkyl), -(C 3 -Cyheterocycloalkyl), (C 5 - Cyheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C 3 -C6 cycloalkyl) may be optionally substituted by one or more group(s)
  • R H and R J are at each occurrence independently -H, Ci-C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo; alternatively, R H and R J may form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , -CN, halo, NR°R P , Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, -OR M , -CN or
  • R L is at each occurrence independently -H, Ci -C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo;
  • R M is at each occurrence independently -H, Ci -C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group hydroxyl, -CN or halo;
  • R° and R P are each independently Ci-C 6 alkyl or Ci-C 6 cycloalkyl, optionally substituted by a group C 1 -C 3 alkyl, -OR Q , -CN or halo; alternatively, R° and R P may form together with the nitrogen atom to which they are attached a 4-8- membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR Q , -CN, halo, Ci-C 6 alkyl or C 3 -C7 cycloalkyl, and which 6-8- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C 3 -C6 cycloalkyl;
  • R Q is -H, -CH 3 or -C 2 H 5 ;
  • z 1 , z 2 , z 3 , and z 4 are independently selected in the group consisting of: C, N, S, O, a group -CH-, and a group -NH-, in such a combination that the resulting ring formed is an aromatic system;
  • R 19 selected in a group consisting of -CH 3 and R 20 selected in the group consisting of -F, -CH 3 , -C 2 H 5 , -CH 2 OH, -CH 2 OMe, -CF 2 CF 3 , -CH 2 SCH 3 , -SCH 3 and -SC 2 H 5 and R 21 selected from -CH 3 and -C 2 H 5 ;
  • A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms fused to a phenyl ring or monocyclic heteroaryl,
  • R 11 is -NR A R B , -N(R C )(C 2 -C 6 alkylene)-NR A R B , -N(R C )(C 3 - C 7 cycloalkylene)-NR A R B , -(Ci-C 6 alkylene)-NR A R B , -(C 3 -C 7 cycloalkylene)-NR A R B , -0-(C 2 -C 6 alkylene)-NR A R B , -0-(C 3 -C 7 cycloalkylene)-NR A R B , -S-(C 2 -C 6 alkylene)- NR A R B , -S-(C 3 -C 7 cycloalkylene)-NR A R B , -N(R c )C(0)-(Ci-C 6 alkylene)-NR A R B , -N(R c )C(0)-(C 3 -C 7 cycloalky
  • R A and R B are at each occurrence independently form together with the nitrogen atom to which they are attached a azetidine or a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is substituted by -NR A R B , - (Ci-C 5 alkylene)-NR A' R B' , -0-(Ci-C 5 alkyl)-NR A R B' , -0-(C 3 -C 7 cycloalkyl)-NR A R B' , -N(R c' )C(0)-(Ci-C 6 alkylene)-NR A' R B' , -N(R c' )C(0)-(C 3 -C 7 cycloalkylene)-NR A R B' , -C(0)N(R c' )-(C 2 -C 6 alkylene)-NR A' R B' , -C(0)N(R c' )-(C 3 -C
  • R 11 is linked to X 4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is substituted by two or more group(s) selected from: -N(R C )(C 2 -Cealkylene)-NR A R B , -N(R c )(C 3 -C 7 cycloalkylene)-NR A R B , -(Ci-C 6 alkylene)-NR A R B , -(C 3 -
  • R A and R B are at each occurrence independently hydrogen, Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, -OR D' , -CN, -NR A R B' or halo; alternatively, R A and R B , may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -OR D , -CN, -NR A R B , halo, Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci -C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7
  • R 18 is selected in the group consisting of aryl, heteroaryl, -(Ci-C 6 alkyl), -(C 3 -C 7 cycloalkyl), -(C 3 -C 7 heterocycloalkyl), (C 5 -Cvheterocycloalkyl)-(Ci-C 6 alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C 6 alkyl),-(C 3 -Cycycloalkyl), -(C 3 -Cyheterocycloalkyl), (C 5 - Cyheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl) is substituted by a group selected from NR H R J , -N(R
  • R A' , R B' , R c , R c' , R D , R D' , R H , R J , R L , R M , R° and R P are as defined above.
  • the invention includes pharmaceutical compositions comprising a compound of the invention, together with one or more pharmaceutically acceptable carriers and/or excipients.
  • Particularly preferred are compositions adapted for inhalation for pulmonary administration.
  • the invention includes the use of a compound of the invention for the treatment of diseases or conditions which benefit from inhibition of p38 MAP kinase activity.
  • the treatment of obstructive or inflammatory airways diseases is a preferred use. All forms of obstructive or inflammatory airways diseases are potentially treatable with the compounds of the present invention, in particular an obstructive or inflammatory airways disease that is a member selected from the group consisting of chronic eosinophilic pneumonia, asthma, COPD, COPD that includes chronic bronchitis, pulmonary emphysema or dyspnea associated or not associated with COPD, COPD that is characterized by irreversible, progressive airways obstruction, adult respiratory distress syndrome (ARDS), exacerbation of airways hyper-reactivity consequent to other drug therapy and airways disease that is associated with pulmonary hypertension, chronic inflammatory diseases including cystic fibrosis, bronchiectasis and pulmonary fibrosis (Idiopathic). Efficacy is anticipated
  • halogen or halo include fluorine, chlorine, bromine and iodine atoms.
  • C x -C y alkyl refers to a straight or branched chain alkyl radical having from x to y carbon atoms.
  • ⁇ is 1 and y is 6, for example, the term includes methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl.
  • C x -C y haloalkyl refers to the above "C x -C y alkyl” group wherein one or more hydrogen atoms are replaced by one or more halogen atoms.
  • C x -C y hydroxyalkyl refers to the above “Cx- Cyalkyl” group wherein one hydrogen atom is replaced by one hydroxyl group.
  • C x -C y alkylene wherein x and y are integers, refers to a C x -C y alkyl radical having in total two unsatisfied valencies, such as a divalent methylene radical.
  • Carbocyclic refers to a mono-, bi- or tricyclic radical having up to 16 ring atoms, all of which are carbon, and includes aryl and cycloalkyl.
  • C z -C k Cycloalkyl wherein z and k are integers refers to a monocyclic saturated carbocyclic radical having from z to k carbon atoms and includes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.
  • C z -C k Cycloalkyl are those radicals having two unsatisfied valencies on the same carbon atom which will link to any C x -C y alkyl, C x -C y alkylene C z -C k Cycloalkyl C z -C k Cycloalkylene, C z - C k heterocycloalkyl, C z -C k heterocycloalkylC x -C y alkyl, C z -C k heterocycloalkylC z - C k Cycloalkyl or (C z -C k )heterocycloalkylcarbonyl group by replacement of two hydrogen atoms placed on the same carbon.
  • this radical forms a g em- di substituted or spiro system together with the C x -C y alkyl, Cx- C y alkylene C z -C k Cycloalkyl C z -C k Cycloalkylene, C z - C k heterocycloalkyl, C z - C k heterocycloalkylC x -C y alkyl, C z -C k heterocycloalkylC z -C k Cycloalkyl or (C z - C k )heterocycloalkylcarbonyl group it is linked to.
  • C z -C k Cycloalkylene radical refers to a C z -C k Cycloalkyl radical having two unsatisfied valencies on different cycle carbon atoms as follows: *
  • aryl refers to a mono- or bi-cyclic carbocyclic aromatic radical, and includes radicals having two monocyclic carbocyclic aromatic rings which are directly linked by a covalent bond.
  • Illustrative of such radicals are phenyl, biphenyl and naphthyl.
  • heteroaryl refers to a mono- or bicyclic aromatic radical containing one or more heteroatoms selected from S, N and O, and includes radicals having two such monocyclic rings, or one such monocyclic ring and one monocyclic aryl ring, which are fused through a common bond.
  • Illustrative examples of 5,6-membered heteroaryl are: are thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, isothiazolyl, pyrazolyl, oxazolyl, isoxazolyl, isothiazolyl, triazolyl, thiadiazolyl, oxadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, and triazinyl.
  • Illustrative examples of 8, 10-membered heteroaryl are: benzothienyl, benzo furyl, benzimidazolyl, benzothiazolyl, benzisothiazolyl, benzoxazolyl, benzisoxazolyl, benzotriazolyl, indolyl and indazolyl.
  • heterocyclyl or “heterocyclic” and relates to a saturated mono-, bi- or tri-cyclic non-aromatic radical containing one or more heteroatoms selected from S, N and O.
  • bicyclic heterocyclic systems included within the scope of the term are fused, spiro and bridged bicyclic systems.
  • C z -C k heterocycloalkyl refers to monocyclic (C z - C k )cycloalkyl groups, in which at least one ring carbon atom is replaced by a heteroatom (e.g. N, NH, S or O).
  • Examples of (C z -C k )heterocycloalkyl include pyridonyl, pyrrolidinyl, thiazolidinyl, piperazinyl, piperidinyl, morpholinyl, thio morpho liny 1.
  • C z -C k heterocycloalkylene refers to a divalent Cz-
  • C k heterocycloalkyl radical wherein C z -C k heterocycloalkyl is as above defined.
  • C z -C k heterocycloalkylC x -C y alkyl refers to the above "C x -C y alkyl” group wherein one or more hydrogen atoms are replaced by one or more "C z - C k heterocycloalkyl” groups.
  • C z - C k heterocycloalkylC x -C y alkyl are systems where two hydrogen atoms linked to the same carbon atom in "C x -C y alkyl” group are replaced by one "C z - C k heterocycloalkyl” group.
  • Such radical thus form a gem-disubstituted "C z - C k heterocycloalkylC x -C y alkyl” system, such as a 1 ,2-dimethyl-pyrrolidin-2-yl radical.
  • C z -C k heterocycloalkylC z -C k Cycloalkyl refers to the above "C z - C k Cycloalkyl” group wherein one or more hydrogen atoms are replaced by one or more "C z -C k heterocycloalkyl” groups.
  • (C z -C k )cycloalkylcarbonyl refers to (C z -C k )cycloalkyl-CO- groups wherein the group "(C z -C k )cycloalkyl” has the meaning above defined.
  • (C z -C k )heterocycloalkylcarbonyl refers to (C z - C k )heterocycloalkyl-CO- groups wherein the group “(C z -C k )heterocycloalkyl” has the meaning above defined.
  • Compounds of the invention may exist in one or more geometrical, optical, enantiomeric, diastereomeric and tautomeric forms, including but not limited to cis- and trans-forms, E- and Z-forms, R-, S- and meso-forms. Unless otherwise stated a reference to a particular compound includes all such isomeric forms, including racemic and other mixtures thereof. Where appropriate such isomers can be separated from their mixtures by the application or adaptation of known methods (e.g. chromatographic techniques and recrystallisation techniques). Where appropriate such isomers may be prepared by the application of adaptation of known methods (e.g. asymmetric synthesis).
  • salt includes base addition, acid addition and ammonium salts.
  • compounds of the invention which are acidic can form salts, including pharmaceutically acceptable salts, with bases such as alkaline metal hydroxides, e.g. sodium and potassium hydroxides; alkaline earth metal hydroxides e.g. calcium, barium and magnesium hydroxides; with organic bases e.g.
  • salts including pharmaceutically acceptable salts with inorganic acids, e.g. with hydrohalic acids such as hydrochloric or hydrobromic acids, sulphuric acid, nitric acid or phosphoric acid and the like, and with organic acids e.g.
  • acetic formic, trifluoroacetic, tartaric, succinic, fumaric, maleic, malic, salicylic, citric, methanesulphonic, p-toluenesulphonic, benzoic, benzenesulfonic, glutamic, lactic, and mandelic acids and the like.
  • Those compounds (I) which have a basic nitrogen can also form quaternary ammonium salts with a pharmaceutically acceptable counter-ion such as ammonium, chloride, bromide, acetate, formate, p-toluenesulfonate, succinate, hemi- succinate, naphthalene-bis sulfonate, methanesulfonate, trifluoroacetate, xinafoate, and the like.
  • a pharmaceutically acceptable counter-ion such as ammonium, chloride, bromide, acetate, formate, p-toluenesulfonate, succinate, hemi- succinate, naphthalene-bis sulfonate, methanesulfonate, trifluoroacetate, xinafoate, and the like.
  • the compounds may also be administered in the form of prodrugs thereof.
  • prodrugs As Novel Delivery Systems, Vol. 14, ACS Symposium Series (T. Higuchi and V.J. Stella) and Bioreversible Carriers in Drug Design, Pergamon Press, 1987 (ed. E. B. Roche, American Pharmaceutical Association; C.S. Larsen and J. 0stergaard, Design and application of prodrugs, In Textbook of Drug Design and Discovery, 3 rd Edition, 2002, Taylor and Francis).
  • Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of formula (I) with certain moieties known to those skilled in the art as 'pro -moieties' as described, for example, in Design of Prodrugs by H. Bundgaard (Elsevier, 1985).
  • compounds of formula (la) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which is linked to group W and identified with number (1) herebelow, possess the absolute configuration herebelow represented:
  • compounds of formula (lb) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which are linked to group W and Y and identified, respectively, with numbers (1) and (2) herebelow, possess the absolute configuration herebelow represented
  • compound of formula (Ic) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which are linked to group W and Y and identified, respectively, with numbers (1) and (2) herebelow, possess the absolute configuration herebelow represented:
  • W is NH or O. In a further embodiment, W is NH.
  • Y is a group -S(0) p -, a group -0(CR 3 R 4 ) n -, a group -(CR 5 R 6 ) lake-, or a group -NR 7 -; p is zero and n is 0, 1 or 2.
  • Y is a group -S(0) p - or a group -0(CR 3 R 4 ) n or; p is zero and n is 0 or 1.
  • Y is a group -0(CR 3 R 4 ) n - and n is 0.
  • R 3 , R 4 , R 5 and R 6 are each independently -H, fluorine or Ci-C 6 alkyl. In another embodiment, R 3 , R 4 , R 5 and R 6 are -H.
  • R 7 is -H, Ci-C 6 alkyl, or C3-C7 cycloalkyl.
  • R 7 is -H.
  • A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms; said cycloalkylene ring being attached to W and Y, and optionally fused to a phenyl ring or to a monocyclic heteroaryl ring having 5 or 6 ring atoms, such phenyl or heteroaryl ring being optionally substituted by one or two groups R 24 .
  • A is group selected in the group consisting of:
  • A is
  • A is group
  • R 24 is not present or, if present, is independently selected from the group consisting of C 1 -C 2 alkyl,-F, -CI and -CN; in a further embodiment, R 24 is not present or, if present, is at each occurrence independently methyl or -F. In a further embodiment, R 24 is not present.
  • the group (Ila) is a group of formula (Ilaa) or (Ilab) which is connected to the group Y through one of the carbons as below indicated
  • the group (Ila) is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom.
  • X 5 is a nitrogen atom.
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is nitrogen.
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is group -CH-.
  • X 4 is a nitrogen atom
  • X 5 is a group -CH- atom
  • X 3 is a carbon atom
  • X 2 is group -CH-.
  • R 13 is -H, Ci_C 6 alkyl or halo.
  • the group (Ila) is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-
  • R 13 is -H.
  • R 11 is selected from a group consisting of -NR A R B , -N(R C )(C 2 -C 6 alkylene)-NR A R B , -N(R c )(C 3 -C 7 cycloalkylene)-NR A R B , -(Ci- C 6 alkylene)-NR A R B , -(C 3 -C 7 cycloalkylene)-NR A R B , -0-(C 2 -C 6 alkylene)-NR A R B , -O- (C 3 -C 7 cycloalkylene)-NR A R B , OR c and -(C 3 -C 7 heterocycloalkyl), wherein any of such -(Ci-Cealkylene)- -(C 2 -C 6 alkylene)-, -(C 3 -C 7 cycloalkylene)- portion in the above listed groups may be optionally substituted by one, two or three groups
  • R 11 is a group
  • R 25 is optionally present and represents one, two or three substituents independently selected in the list consisting of Ci-C 6 alkyl, (Ci-C 3 ) haloalkyl, (Ci- C 4 )hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 .
  • R 11 is a group
  • R represents one, two or three substituents independently selected in the list consisting of Ci-C 6 alkyl, (C 1 -C 3 ) haloalkyl, (Ci-C4)hydroxyalkyl, C 3 -C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 .
  • R 11 is a group
  • R represents one or two Ci-C 6 alkyl substituents; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 .
  • R 11 is a group
  • R 11 is a group
  • R 11 is a group
  • R 11 is phenyl which is optionally substituted by one or more group(s) selected from halo, Ci-C 6 alkyl which is substituted by one or more group(s) -CN; -(Ci-Cealkylene)-NR A R B , wherein R A and R B may form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups selected from -OR D , -CN, -NR C R D , halo, Ci-C 6 alkyl or C 3 -C 7 cycloalkyl; and which 6-1 1- membered saturated heterocyclic monocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C 3 -C6 cycloalkyl.
  • R 25 is one, two or three groups independently selected in the list consisting of Ci-C 6 alkyl, (C 1 -C 3 ) haloalkyl, (Ci-C4)hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxyl and halo.
  • R A and R B form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more groups independently selected from -OR D , -CN, -NR A R B' , halo, Ci-C 6 alkyl, -(C 3 - Cy) cycloalkyl-NR A' R B' , -(Ci-C 5 alkylene)-NR A R B' , -0-(Ci-C 5 alkylene)-NR A R B' and -0-(C 3 -C 7 cycloalkyl)-NR A' R B' , -N(R c' )C(0)-(Ci-C 6 alkylene)-NR A' R B' , -N(R c' )C(0)-(C 3 -C 7 cycloalkylene)-NR A' R B' , -C
  • R A and R B form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups independently selected from Ci-C 6 alkyl, -(C 1 -C 5 alkylene)-NR A R B' and -0-(Ci-C 5 alkylene)-NR A R B such Ci-C 6 alkyl being optionally substituted by a group C 1 -C3 alkyl or halo.
  • R A and R B form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C 1 -C 5 alkylene)-NR A R B , wherein R A and R B are independently -H or Ci-C 6 alkyl optionally substituted by a group C 1 -C3 alkyl.
  • R 17 is a group of general formula (IV)
  • R 19 is selected in a group consisting of -H, -CH 3 , -CN and OH;
  • R 20 is selected in the group consisting of: -H, -F, -CH 3 , -C2H5 and -CH 2 OH;
  • R 21 is -H,
  • R 1 i 9 y is -H
  • R 2 z 0 u and R 21 are -CH3.
  • R and R are H and R Z1 is -CH 3 .
  • R 19 , R 2 z 0 u and R 21 are -H.
  • R 19 , R 20 and R 21 are -CH3.
  • R 18 is absent or is selected in the group consisting of aryl, heteroaryl and -(Ci-C 6 alkyl) wherein any of such aryl, heteroaryl and -(Ci-C 6 alkyl) may be optionally substituted by one or more group(s) selected from -OH, -Ci- C 6 alkyl, C 3 -C 6 cycloalkyl, -0-(Ci-C 6 alkyl), -0-(C 3 -C 6 cycloalkyl), -NR H R J , -N(R L )(C 2 -C 6 alkylene)-NR H R J , -N(R L )(C 3 -C 7 cycloalkylene)-NR H R J , -(Ci- C 6 alkylene)-NR H R J , -(C 3 -C 7 cycloalkylene)-NR H R J , -0-(C 2 -C 6 alkylene)-NR H
  • R 18 is aryl optionally substituted by one or more group(s) selected from -Ci-C 6 alkyl, -(Ci-C 6 alkylene)-NR H R J , -0-(C 2 -C 6 alkylene)-NR H R J wherein any of such Ci-C 6 alkyl, -(Ci-Cealkylene)- and -(C 2 -Cealkylene)- portion in the above listed groups may be optionally substituted by a group Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, -OR L or halo.
  • R H and R J are at each occurrence independently Ci-C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo; alternatively, R H and R J may form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , -CN, halo, NR°R P , Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 -C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, -OR M , -CN or halo;
  • R H and R J may form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , NR°R P or Ci-C 6 alkyl wherein such Ci-C 6 alkyl is optionally substituted by a group C 1 -C 3 alkyl, -OR M or halo; and which 6-1 1- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C 3 -C 6 cycloalkyl, wherein any of such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl may be optionally substituted by a group Ci-C 6 alkyl, C 3 -C7 cycloalkyl, -OR M , -CN or halo.
  • R L is independently -H, Ci -C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group C 1 -C 3 alkyl, -OR M , -CN or halo.
  • R M is independently -H, Ci -C 6 alkyl or C 3 -C 6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group hydroxyl, -CN or halo.
  • R° and R P are each independently Ci-C 6 alkyl or Ci-C 6 cycloalkyl, optionally substituted by a group C 1 -C 3 alkyl or -OR Q ; alternatively, R° and R P may form together with the nitrogen atom to which they are attached a 4-8- membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR Q , -CN, halo, Ci-C 6 alkyl or C 3 -C7 cycloalkyl, and which 6-8- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C 3 -C6 cycloalkyl.
  • R° and R P are each independently Ci-C 6 alkyl or Ci- C 6 cycloalkyl, optionally substituted by a group C 1 -C 3 alkyl.
  • R Q is -H, -CH 3 or -C 2 H 5 .
  • R 2 is a radical of formula (Ilia)
  • R 19 , R 20 and R 21 are -CH 3 .
  • R is a radical of formula (Ilia)
  • R 19 is -H
  • R 20 and R 21 are -CH 3 .
  • R 2 is a radical of formula (Ilia)
  • R iy and R 2U are H and R is -CH 3 .
  • R 2 is a radical of formula (Ilia)
  • R 19 , R 20 and R 21 are -H.
  • R 2 is a radical of formula (Ilia)
  • R , R zu and R Z1 are -CH 3 and R is absent or is selected from -(Ci- C 6 alkyl), -(Ci-C 6 alkylene)-NR H R J , -0-(C 2 -C 6 alkylene)-NR H R J or aryl, wherein any portion in the above listed groups may be optionally substituted by a group Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, -OR L or halo.
  • R is a radical of formula (Ilia)
  • R i y is -H
  • R zu and R Z 1 are -CH 3 and R is heteroaryl substituted by a
  • R H and R J are independently Ci-C 6 alkyl optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo; alternatively, R H and R J may form together with the nitrogen atom to which they are attached a 4- 1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , halo, NR°R P , Ci-C 6 alkyl or C 3 -C 7 cycloalkyl; and which 6- 1 1 - membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl optionally substituted by a group Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, -CN, or halo.
  • R 2 is a radical of formula (Ilia)
  • R i y and are -H, and R is -CH 3 and R is heteroaryl substituted by a -(Ci-C 6 alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl optionally substituted by a group C 1 -C 3 alkyl, -CN or halo.
  • R 2 is a radical of formula (Ilia)
  • R , R zu and R Z 1 are -CH 3 and R is aryl or heteroaryl optionally substituted by a group selected from -(Ci-C 6 alkyl), -0-(Ci-C 6 alkylene)-NR H R J or - (Ci-C 6 alkylene)-NR H R J and wherein A is a group
  • R is a radical of formula (Ilia)
  • R , R zu and R Z 1 are -CH 3 and R is aryl optionally substituted by -(Ci-Cealkyl), -(C 2 -C 6 alkylene)-NR H R J or -0-(C 2 -C 6 alkylene)-NR H R J .
  • R is a radical of formula (Ilia)
  • R , R zu and R Z 1 are H and R is phenyl optionally substituted by a -0-(C 2 -C 6 alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl optionally substituted by a group C 1 -C3 alkyl, -CN or halo.
  • R 2 is a radical of formula (Ilia)
  • R , R zu and R Z 1 are H and R is phenyl optionally substituted by -O- (C 2 -C 6 alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl.
  • compounds of formula (I A) wherein W is NH, Y is a group -0(CR 3 R 4 - and n is 0, A is group
  • R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • R 11 is a group: wherein R represents one, two or three substituents independently selected in the list consisting of: Ci-C 6 alkyl, (C 1 -C 3 ) haloalkyl, (Ci-C4)hydroxyalkyl, C 3 -C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ;
  • R 2 is a radical of formula (Ilia)
  • R , R zu and R Z 1 are -CH 3 and wherein R is phenyl optionally substituted by a group selected from Ci-C 6 alkyl, -(C 2 -Cealkylene)-NR H R J and -O- (C 2 -C 6 alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl optionally substituted by a group C 1 -C 3 alkyl, -CN or halo.
  • compounds of formula (IB) wherein W is NH, Y is a group -0(CR 3 R 4 - and n is 0, A is group
  • R 1 is a group of formula Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-
  • R 13 is -H
  • R 11 is a group -NR A R B , wherein R A and R B form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C 1 -C5 alkylene)-NR A R B' , wherein R A ' and R B ' are independently -H or Ci-C 6 alkyl optionally substituted by a group C 1 -C3 alkyl, C3-Cycycloalkyl, -OR D , -CN or halo; or R is a group wherein R represents one or two substituents independently selected in the list consisting of Ci-C 6 alkyl, (C 1 -C3) haloalkyl, (Ci-C4)hydroxyalkyl
  • R 19 , R2 z 0 u and R 2 Z 1 1 are -CH 3 and wherein R 18 is heteroaryl optionally substituted by a group selected from Ci-C 6 alkyl, -(C 2 -Cealkylene)-NR H R J and -O- (C 2 -C 6 alkylene)-NR H R J , wherein R H and R J form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , -CN, halo, NR°R P , Ci-C 6 alkyl or C 3 -C 7 cycloalkyl, such Ci-C 6 alkyl and C 3 - C 7 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, C 3 -C 7 cycloalkyl, wherein R° and R P are each independently Ci-C 6 al
  • compounds of formula (IC) wherein W is NH, Y is a group -0(CR 3 R 4 ) n - and n is 0,
  • A is group:
  • R is a group: wherein R 25 is optionally present and represents one or two substituents independently selected in the list consisting of: Ci-C 6 alkyl, (Ci-C 3 ) haloalkyl, (Ci- C4)hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ;
  • R 2 is a radical of formula (Ilia)
  • R 19 , R 20 and R 21 are H and R 18 is phenyl optionally substituted by -O- (Ci-C 6 alkylene)-NR H R J , wherein R H and R J are Ci-C 6 alkyl.
  • compounds of formula (ID) wherein W is NH, Y is a group -0(CR 3 R 4 ) n - and n is 0, A is group
  • R 1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjace
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-
  • R 13 is -H
  • R 11 is a group: wherein R 25 is optionally present and represents one two or three substituents independently selected in the list consisting of: Ci-C 6 alkyl, (C1-C3) haloalkyl, (Ci- C 4 )hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ; wherein R 2 is a radical of formula (Ilia): (Ilia)
  • R 1 i 9 y is -H
  • R 2 z 0 u and R 2 Z 1 1 are -CH 3
  • R 18 is heteroaryl substituted by a -(Ci-C 6 alkylene)-NR H R J
  • R H and R J are independently Ci-C 6 alkyl optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo; alternatively, R H and R J may form together with the nitrogen atom to which they are attached a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -OR M , halo, NR°R P , Ci-C 6 alkyl or C 3 -C 7 cycloalkyl; and which 6-1 1- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl optionally substituted by a group Ci-
  • Y is a group -0(CR 3 R 4 ) n - and n is 0,
  • A is group
  • R 1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-, and R 13 is -H; wherein R 11 is a group: wherein R is optionally present and represents one two or three substituents independently selected in the list consisting of: Ci-C 6 alkyl, (C 1 -C 3 ) haloalkyl, (Ci- C4)hydroxyalkyl, C 3 -C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ; wherein R 2 is a radical of formula (I
  • R and R are H and R is -CH 3 and R is heteroaryl substituted by a -(Ci-C 6 alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl optionally substituted by a group C 1 -C3 alkyl, -CN or halo.
  • compounds of formula (IF) wherein W is NH, Y is a group -0(CR 3 R 4 ) n - and n is 0,
  • A is group wherein R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-, and R 13 is -H; wherein R 11 is a group -NR A R B , wherein R A and R B form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C 1 -C5 alkylene)-NR A R B' , wherein R A ' and R B ' are independently -H or Ci-C 6 alkyl optionally substituted by a group C 1 -C3 alkyl, C 3 -C 7 cycloalkyl, -OR D' , -CN or halo; wherein R 2 is a radical of formula (Ilia)
  • R Z1 are - and wherein R is -Ci-C 6 alkyl, optionally substituted by Ci-C 6 alkyl, C 3 -C 7 cycloalkyl, -OR L or halo.
  • compounds of formula (IG) wherein W is NH, Y is a group -0(CR 3 R 4 ) n - and n is 0,
  • A is group wherein R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X 2
  • X 4 is a carbon atom
  • X 5 is a nitrogen atom
  • X 3 is a nitrogen atom
  • X 2 is a group -CH-, and R 13 is -H; wherein R 11 is phenyl which is optionally substituted by one or more group(s) selected from halo, Ci-C 6 alkyl, wherein such Ci-C 6 alkyl is optionally substituted by -(Ci-Cealkylene)-NR A R B , wherein R A and R B may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups selected from -OR D , -CN, -NR C R D , halo, Ci-C 6 alkyl or C3-C7 cycloalkyl; and which 6- 1 1 - membered saturated heterocyclic monocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C 6 alkyl or C3-C6 cycloalkyl; wherein R 2 is
  • compounds of formula (IH) wherein W is NH, Y is a group -0(CR 3 R 4 ) n - and n is 0, A is group: wherein R 1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X
  • X is a carbon atom, X is a nitrogen atom, X is a nitrogen atom and X 2 is a group -CH-, and R 13 is -H; wherein R 11 is a group wherein R represents one or two substituents independently selected in the list consisting of Ci-C 6 alkyl, (Ci-C 3 ) haloalkyl, (Ci-C4)hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ; wherein R 2 is a radical of formula (Ilia)
  • R 19 , R2 z 0 u and R 2 Z 1 1 are -CH 3 and wherein 18
  • R is aryl or heteroaryl optionally substituted by a group selected from Ci-C 6 alkyl, -(C 2 -Cealkylene)-NR H R J and -0-(C 2 -C6alkylene)-NR H R J , wherein R H and R J are independently Ci-C 6 alkyl or C 3 -C6 cycloalkyl, such Ci-C 6 alkyl or C 3 -C 6 cycloalkyl being optionally substituted by a group Ci-C 3 alkyl, -OR M , -CN or halo.
  • compounds of formula (IL) wherein W is NH, Y is a group -0 CR 3 R 4 ) n - and n is 0, A is group
  • R is a group: wherein R is optionally present and represents one or two substituents independently selected in the list consisting of: Ci-C 6 alkyl, (Ci-C 3 ) haloalkyl, (Ci- C4)hydroxyalkyl, C 3 -C 7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R 1 1 to the rest of the molecule via X 4 ;
  • R 2 is a radical of formula (Ilia)
  • R , R zu and R Z1 are CH 3 and R 18 is phenyl optionally substituted by a group selected from Ci-C 6 alkyl, -(C 2 -Cealkylene)-NR H R J and -0-(C 2 -Cealkylene)- NR H R J , wherein R H and R J are Ci-C 6 alkyl or R H and R J form together with the nitrogen atom to which they are attached an azetidine or a 4-11 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups halo or NR°R P , wherein R° and R P are each independently Ci-C 6 alkyl.
  • a compound of formula (I) is selected in the list consisting of:
  • the compounds of the invention are p38MAPK inhibitors, and thus may have utility for the treatment of diseases or conditions which benefit from inhibition of the p38 enzyme.
  • diseases and conditions are known from the literature and several have been mentioned above.
  • the compounds are generally of use as anti-inflammatory agents, particularly for use in the treatment of respiratory disease.
  • the compounds may be used in the treatment of chronic obstructive pulmonary disease (COPD), chronic bronchitis, lung fibrosis, pneumonia, acute respiratory distress syndrome (ARDS), pulmonary emphysema, or smoking-induced emphysema, intrinsic (non-allergic asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, steroid resistant asthma, neutrophilic asthma, bronchitic asthma, exercise induced asthma, occupational asthma and asthma induced following bacterial infection, cystic fibrosis, pulmonary fibrosis and bronchiectasis.
  • COPD chronic obstructive pulmonary disease
  • ARDS acute respiratory distress syndrome
  • pulmonary emphysema or smoking-induced emphysema
  • intrinsic (non-allergic asthma and extrinsic (allergic) asthma mild asthma, moderate asthma, severe asthma, steroid resistant asthma
  • neutrophilic asthma bronchitic asthma
  • exercise induced asthma occupational asthma
  • the present invention provides the use of the compounds of the invention for the prevention and/or treatment of any disease or condition which benefit from inhibition of the p38 enzyme.
  • the present invention provides the use of compounds of the invention for the preparation of a medicament for the prevention and/or treatment of any disease or condition which benefit from inhibition of the p38 enzyme.
  • the present invention provides a method for prevention and/or treatment of any disease which benefit from inhibition of the p38 enzyme, said method comprises administering to a patient in need of such treatment a therapeutically effective amount of a compound of the invention.
  • the compounds with which the invention is concerned are p38 kinase inhibitors, and are useful in the treatment of several diseases for example inflammatory diseases of the respiratory tract.
  • diseases for example inflammatory diseases of the respiratory tract.
  • diseases include asthma, rhinitis, allergic airway syndrome, bronchitis and chronic obstructive pulmonary disease.
  • the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing treatment. Optimum dose levels and frequency of dosing will be determined by clinical trial, as is required in the pharmaceutical art. In general, the daily dose range for oral administration will lie within the range of from about 0.001 mg to about 100 mg per kg body weight of a human, often 0.01 mg to about 50 mg per kg, for example 0.1 to 10 mg per kg, in single or divided doses.
  • the daily dose range for inhaled administration will lie within the range of from about 0.1 ⁇ g to about lmg per kg body weight of a human, preferably 0.1 ⁇ g to 50 ⁇ g per kg, in single or divided doses. On the other hand, it may be necessary to use dosages outside these limits in some cases.
  • inhaled administration is preferred.
  • compositions may be in the form of tablets, capsules, powders, granules, lozenges, liquid or gel preparations, such as oral, topical, or sterile parenteral solutions or suspensions.
  • Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricant, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants for example potato starch, or acceptable wetting agents such as sodium lauryl sulfate.
  • the tablets may be coated according to methods well known in normal pharmaceutical practice.
  • Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use.
  • Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, glucose syrup, gelatin hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, fractionated coconut oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl /?-hydroxybenzoate or sorbic acid, and if desired conventional flavouring or colouring agents.
  • suspending agents for example sorbitol, syrup, methyl cellulose, glucose syrup, gelatin hydrogenated edible fats
  • emulsifying agents for example lecithin, sorbitan monooleate, or acacia
  • non-aqueous vehicles which may include edible oils
  • almond oil fractionated coconut oil
  • oily esters such as glycerine, propy
  • the drug may be made up into a cream, lotion or ointment.
  • Cream or ointment formulations which may be used for the drug are conventional formulations well known in the art, for example as described in standard textbooks of pharmaceutics such as the British Pharmacopoeia.
  • the active ingredient may also be administered parenterally in a sterile medium.
  • the drug can either be suspended or dissolved in the vehicle.
  • adjuvants such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle.
  • compounds of the invention may also be formulated for inhalation, for example as a nasal spray, or dry powder or aerosol inhalers.
  • the active compound is preferably in the form of microparticles. They may be prepared by a variety of techniques, including spray-drying, freeze-drying and micronisation. Aerosol generation can be carried out using, for example, pressure-driven jet atomizers or ultrasonic atomizers, preferably using propellant-driven metered aerosols or propellant-free administration of micronized active compounds from, for example, inhalation capsules or other "dry powder" delivery systems.
  • a composition of the invention may be prepared as a suspension for delivery from a nebuliser or as an aerosol in a liquid propellant, for example for use in a pressurised metered dose inhaler (PMDI).
  • PMDI pressurised metered dose inhaler
  • Propellants suitable for use in a PMDI are known to the skilled person, and include CFC-12, HFA-134a, HFA-227, HCFC-22 (CC1 2 F 2 ) and HFA-152 (CH 4 F 2 and isobutane).
  • a composition of the invention is in dry powder form, for delivery using a dry powder inhaler (DPI).
  • DPI dry powder inhaler
  • Microparticles for delivery by administration may be formulated with excipients that aid delivery and release.
  • microparticles may be formulated with large carrier particles that aid flow from the DPI into the lung.
  • Suitable carrier particles are known, and include lactose particles; they may have a mass median aerodynamic diameter of greater than 90 ⁇ .
  • the active compounds may be dosed as described depending on the inhaler system used.
  • the administration forms may additionally contain excipients, such as, for example, propellants (e.g. Frigen in the case of metered aerosols), surface-active substances, emulsifiers, stabilizers, preservatives, flavorings, fillers (e.g. lactose in the case of powder inhalers) or, if appropriate, further active compounds.
  • Suitable therapeutic agents for a combination therapy with compounds of the invention include, but are not limited to: (1) corticosteroids, such as fluticasone propionate, fluticasone furoate, mometasone furoate, beclometasone dipropionate, ciclesonide, budesonide, GSK 685698, GSK 870086, QAE 397, QMF 149, TPI-1020; (2) ⁇ 2- adrenoreceptor agonists such as salbutamol, albuterol, terbutaline, fenoterol, and long acting 2-adrenoreceptor agonists such as salmeterol, indacaterol, formoterol (including formoterol fumarate), arformoterol,
  • corticosteroids such as fluticasone propionate, fluticasone furoate, mometasone furoate, beclometasone dipropionate, ciclesonide, budesonide, GSK 68
  • a peptide mucolytic for example recombinant human deoxyribonoclease I (dornase-alfa and rhDNase) or helicidin
  • antibiotics for example azithromycin, tobramycin and aztreonam
  • non-selective COX-1 / COX-2 inhibitors such as ibuprofen or ketoprofen
  • COX-2 inhibitors such as celecoxib and rofecoxib
  • VLA-4 antagonists such as those described in WO97/03094 and WO97/02289
  • TACE inhibitors and TNF-a inhibitors for example anti-TNF monoclonal antibodies, such as Remicade and CDP-870 and TNF receptor immunoglobulin molecules, such as Enbrel
  • inhibitors of matrix metalloprotease for example MMP-12
  • human neutrophil elastas for example recombinant human deoxyribonoclease I (dornase-alfa and
  • the invention is also directed to a kit comprising the pharmaceutical compositions of compounds of the invention alone or in combination with or in admixture with one or more pharmaceutically acceptable carriers and/or excipients and a device which may be a single- or multi-dose dry powder inhaler, a metered dose inhaler or a nebulizer.
  • protecting group designates a protective group adapted to preserve the function of the group it is bound to.
  • protective groups are used to preserve amino, hydroxyl, or carboxyl functions.
  • Appropriate protecting groups may thus include, for example, benzyl, benzyloxycarbonyl, t-butoxycarbonyl, alkyl or benzyl esters or the like, which are well known to those skilled in the art [see, for a general reference, T.W. Green; Protective Groups in Organic Synthesis (Wiley, N.Y. 1981)].
  • Optional salification of the compounds of formula (I) or N-oxides on the pyridine ring thereof may be carried out by properly converting any of the free acidic or amino groups into the corresponding pharmaceutically acceptable salts.
  • the operative conditions being employed for the optional salification of the compounds of the invention are all within the ordinary knowledge of the skilled person.
  • Compounds of general formula (I) may be prepared from compounds of general formula (la) by reaction with a compound of general formula (lbl) or (lb2) wherein R 2 is as defined in general formula (I), in a suitable solvent such as dimethyl sulfoxide, 1 ,4-dioxane, ⁇ , ⁇ -dimethylformamide or acetonitrile, in the presence of a base such as diisopropylethylamme at a range of temperatures, preferably between room temperature and 100°C.
  • a suitable solvent such as dimethyl sulfoxide, 1 ,4-dioxane, ⁇ , ⁇ -dimethylformamide or acetonitrile
  • R is a group of formula (Illb) and R , R , z , z , z and z are as defined above can be prepared from compounds of formula (l e),
  • a suitable reducing agent such as tin (II) chloride, iron, or hydrogen gas with a suitable catalyst such as palladium on carbon
  • a suitable solvent such as methanol, ethanol or acetic acid
  • compounds of general formula (lea) as above defined can be prepared from compounds of formula (If), wherein R 17 , R 18 , z 1 , z 2 , z3 and z 4 are as defined above and wherein PG is a suitable compatible protecting group known to those skilled in the art, such as benzyl, benzyl carbamate or tert-butyl carbamate,
  • suitable deprotection conditions such as hydrochloric acid, trifluoroacetic acid, or hydrogen catalysed by for example palladium on carbon, in a suitable solvent such as dichloromethane, methanol, ethanol or acetic acid, at a range of temperatures, preferably between 0°C and 100°C.
  • PG is a suitable protecting group known to those skilled in the art, such as benzyl, benzyl carbamate or tert-butyl carbamate, using suitable conditions such as in the presence of a base such as potassium carbonate or diisopropylethyl amine or under Buchwald conditions (with a catalyst such as Pd(OAc) 2 , a ligand such as 2,2'- bis(diphenylphosphino)-l , l'-binaphthyl and base such as sodium tert-butoxide), in a suitable solvent such as toluene or tetrahydrofuran, at a range of temperatures, preferably between room temperature and 150°C.
  • Compounds of general formula (la) may be prepared from compounds of general formula (2a) by reaction with a compound of general formula (2d).
  • G is a suitable chemical group known to those skilled in the art selected such that it can facilitate a suitable coupling reaction such as nucleophilic displacement or metal catalysed cross coupling:
  • examples of G may include halogen or a suitable leaving group such as mesylate or triflate either directly linked or attached via a group -(CR 3 R 4 ) n -.
  • Examples of the coupling conditions used may include using a base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone in a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
  • a base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone
  • a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
  • a method to perform this coupling may involve Mitsunobu conditions (diethylazodicarboxylate / triphenylphosphine) in a suitable solvent such as tetrahydrofuran or 1 ,4-dioxane at a range of temperatures preferably between -10°C and 100°C.
  • a method to perform this coupling may be under metal (for example palladium or copper) catalysed coupling conditions in the presence of a suitable ligand such as Xantphos or 1 , 10- phenanthroline in the presence of a base such as caesium carbonate in a suitable solvent such as tetrahydrofuran, 1 ,4-dioxane or ⁇ , ⁇ -dimethylformamide at a range of temperatures preferably between - 10°C and 150°C.
  • a suitable ligand such as Xantphos or 1 , 10- phenanthroline
  • a base such as caesium carbonate
  • a suitable solvent such as tetrahydrofuran, 1 ,4-dioxane or ⁇ , ⁇ -dimethylformamide
  • examples of conditions to perform this coupling may involve the use of a base such as sodium hydride or triethylamine or a coupling reagent such as HATU in a suitable solvent such as dichloromethane, tetrahydrofuran, 1 ,4-dioxane or ⁇ , ⁇ -dimethylformamide at a range of temperatures preferably between -10°C and 150°C.
  • Compounds of general formula (2da) as above defined may be prepared from compounds of general formula (3e) using a suitable oxidant such as chloramine T, lead tetracetate or phenyl iodine(III) diacetate, in a suitable solvent such as dichloromethane or ethanol at a range of temperatures, preferably between room temperature and 100°C.
  • a suitable oxidant such as chloramine T, lead tetracetate or phenyl iodine(III) diacetate
  • a suitable solvent such as dichloromethane or ethanol
  • Compounds of general formula (3e) may be prepared from compounds of general formula (3a) by reaction with an aldehyde of general formula (3c) above reported, in a suitable solvent such as ethanol or tetrahydrofuran at a range of temperatures, preferably between room temperature and 80°C.
  • a suitable solvent such as ethanol or tetrahydrofuran
  • compounds of formula (2da) may be prepared from compounds of formula (3d) using a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazodicarboxylate/triphenylphosphine/trimethylsilylazide), in the absence or presence of a suitable solvent such as tetrahydrofuran, toluene or NMP, at a range of temperatures, preferably between room temperature and 150°C.
  • a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazodicarboxylate/triphenylphosphine/trimethylsilylazide)
  • a suitable solvent such as tetrahydr
  • Compounds of formula (3d) may be prepared from compounds of formula (3a) by reaction with a compound of general formula (3bl) using a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or acetonitrile, at a range of temperatures, preferably between room temperature and 150°C.
  • a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile
  • a base such as diisopropylethylamine
  • a suitable solvent such as dichloromethane or acetonitrile
  • compounds of general formula (2da) may be prepared from compounds of general formula (4c), wherein G 2 represents halogen, using methods such as a metal (for example palladium) catalysed coupling with a suitable R n G 5 derivative wherein G 5 is a group such as boronate acid/ester or stannane in a suitable solvent such as tetrahydrofuran or 1 ,4-dioxane at a range of temperatures preferably between ambient temperature and 150°C.
  • a metal for example palladium
  • An alternative method may involve displacement of said halogen with a suitable group R n H (such as that containing an -NH, -OH or -SH group) in the presence of a base such as sodium hydride, potassium tert-butoxide or ⁇ , ⁇ -diethylisopropylamine in a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
  • a suitable group R n H such as that containing an -NH, -OH or -SH group
  • the group G 2 may be also transformed from groups such as halogen to groups such as -CHO, -COOH, -COOEt and S0 2 C1 by means of metal insertion methods known to those skilled in the art such as palladium catalysis, Grignard formation or lithium halogen exchange.
  • C 7 cycloalkylene)-NR A R B , -S(0) 2 N(R c )-(C 2 -C 6 alkylene)-OR D or -S(0) 2 N(R c )-(C 3 - C 7 cycloalkylene)-OR D may be prepared from compounds of general formula (4c), wherein G 2 represents -CHO, -COOH, -COOEt and -S0 2 C1, by reaction with a suitable amine such as HNR A R B etc using methods such as reductive amination (using a reagent such as sodium triacetoxyboro hydride) or amide/sulphonamide formation in the presence of suitable reagents such as HATU with a base such as ⁇ , ⁇ -diethylisopropylamine or trimethylaluminium in a suitable solvent such as dichloromethane, ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or aceton
  • Compounds of general formula (4c) wherein G 2 is a group such as -COOEt may be synthesised from compounds of general formula (4a) by reaction with a compound such as diethyloxalate in the presence of an acid such as acetic acid at a range of temperatures, preferably between room temperature and 120°C.
  • Compounds of general formula (4c) wherein G 2 is a group such as bromine or chlorine may be synthesised from compounds of general formula (4b) by reaction with a compound such as N-chlorosuccinimide or N-bromosuccinimide in a solvent such as chloroform at a range of temperatures, preferably between -10°C and room temperature.
  • Compounds of general formula (4b) may be synthesised from compounds of general formula (4a) by reaction with a compound such as diethoxymethylacetate at a range of temperatures, preferably between room temperature and 100°C.
  • a suitable alkylating agent R 1 1 in the presence of a base such as caesium carbonate in a suitable solvent such as ⁇ , ⁇ -dimethylformamide at a range of temperatures, preferably between room temperature and 150°C.
  • Compounds of general formula (5e) may be prepared from compounds of general formula (5d) using a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazadicarboxylate / triphenylphosphine / trimethylsilylazide), in the absence or presence of a suitable solvent such as tetrahydrofuran, toluene or NMP, at a range of temperatures, preferably between room temperature and 120°C.
  • a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazadicarboxylate / triphenylphosphine / trimethylsilylazide)
  • a suitable solvent
  • Compounds of general formula (5d) may be prepared from compounds of general formula (5c) by reaction with a compound of general formula (3b 1) as above defined using a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or acetonitrile, at a range of temperatures, preferably between room temperature and 150°C.
  • a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile
  • a base such as diisopropylethylamine
  • a suitable solvent such as dichloromethane or acetonitrile
  • a compound of general formula (3b2) as above defined in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or THF at a range of temperatures preferably between - 10°C and the boiling point of the solvent.
  • a base such as diisopropylethylamine
  • a suitable solvent such as dichloromethane or THF
  • Compounds of general formula (5c) may be prepared from compounds of general formula (5b) wherein G 3 is a suitable leaving group such as halogen, by reaction with a reagent such as hydrazine mo no hydrate in a suitable solvent such as ethanol at a range of temperatures preferably between room temperature and 100°C.
  • Compounds of general formula (5b) may be prepared from compounds of general formula (5a) by reaction with a suitable protecting group reagent known in the art such as Boc anhydride in the presence of a base such as triethylamine in a suitable solvent such as dichloromethane or tetrahydrofuran at a range of temperatures preferably between room temperature and 100°C.
  • a suitable protecting group reagent known in the art such as Boc anhydride
  • a base such as triethylamine
  • a suitable solvent such as dichloromethane or tetrahydrofuran
  • Compounds of general formula (2aa) may be prepared from compounds of general formula (6b) and (6c) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol at a range of temperatures preferably between room temperature and 100°C.
  • Compounds of general formula (6b), wherein PG is a protecting group, preferably trifluoroacetamide, and the group -OH is placed on the cycloalkylene portion of ring A may be prepared from compounds of general formula (6a) by using a chiral reductive method such as using formic acid and RuCl[S,S-Tsdpen(p- cymene)] in the presence of a base such as triethylamine in a solvent such as N,N- dimethylformamide at a range of temperatures preferably between room temperature and 150°C. It will be recognised that compounds of formula (6a) may be homochiral as illustrated or be the opposite enantiomer or racemic.
  • a chiral reductive method such as using formic acid and RuCl[S,S-Tsdpen(p- cymene)] in the presence of a base such as triethylamine in a solvent such as N,N- dimethylformamide at a range of temperatures preferably
  • Compounds of general formula (2ab) may be prepared from compounds of general formula (7a) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol or trifluoroacetic acid in dichloromethane at a range of temperatures preferably between room temperature and 100°C.
  • Compounds of formula (7a) may be prepared from compounds of general formula (6a) and amines (7b) by reaction under reductive amination conditions, using a reducing agent such as sodium triacetoxyboro hydride and a solvent such as 1 ,2-dichloroethane at a range of temperatures preferably between room temperature and 100°C.
  • a reducing agent such as sodium triacetoxyboro hydride
  • a solvent such as 1 ,2-dichloroethane
  • G is a suitable leaving group such as halogen or mesylate which can react with a suitable amine (7b) in the presence of a suitable base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro- 2(lH)-pyrimidinone in a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
  • a suitable base such as sodium hydride or potassium tert-butoxide
  • a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°
  • Compounds of formula (7c) can be prepared from compounds of formulae (6b) or (6c) using halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane or activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropy lamine .
  • halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane
  • activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropy lamine .
  • a suitable solvent such as dimethyl sulfoxide, 1 ,4-dioxane, ⁇ , ⁇ -dimethylformamide or acetonitrile
  • Compounds of formula (lab) as above defined may be prepared from compounds of formula (8g) using deprotection conditions such as hydrazine in methanol at a range of temperatures preferably between room temperature and the boiling point of the solvent.
  • Examples of the coupling conditions used may include using a base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone in a suitable solvent such as ⁇ , ⁇ -dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
  • Compounds of formula (8f) can be prepared from compounds of formula (8e) using dithiothreitol, monopotassium phosphate, potassium carbonate in a solvent such as methanol in the presence of acetic acid at a range of temperatures preferably between room temperature and the boiling point of the solvent.
  • Compounds of formula (8e) can be prepared from compounds of formula (8d) using 2-nitrobenzenesulfenyl chloride in acetic acid at a range of temperatures preferably between room temperature and 100°C.
  • Compounds of formula (8d) can be prepared from compounds of formula (8c) using phthalimide, triphenylphosphine and diisopropyl azodicarboxylate in a solvent such as tetrahydrofuran at a range of temperature preferably between 0°C and the boiling point of the solvent.
  • Compounds of formula (8c) can be prepared from compounds of formula (8b) using a reducing agent such as sodium boro hydride in a solvent such as methanol at a range of temperatures preferably between 0°C and the boiling point of the solvent.
  • a reducing agent such as sodium boro hydride in a solvent such as methanol
  • Compounds of formula (8b) can be prepared from compounds of formula (8a) using tert-butanethiol in the presence of a base such as diisopropylethylamine in a solvent such as tetrahydrofuran at a range of temperatures preferably between 0°C and the boiling point of the solvent.
  • a base such as diisopropylethylamine
  • a solvent such as tetrahydrofuran
  • Compounds of general formula (lab) may be prepared from compounds of general formula (9b) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol at a range of temperatures preferably between room temperature and 100°C.
  • Compounds of formula (9b) may be prepared from compounds of general formula (9a) by reaction with a suitable reduction agent for example hydrogen gas in the presence of a suitable catalyst such as palladium on activated charcoal in a suitable solvent such as ethanol at a range of temperatures between room temperature and 70°C and pressures between atmospheric and 4 Barr.
  • a suitable reduction agent for example hydrogen gas in the presence of a suitable catalyst such as palladium on activated charcoal in a suitable solvent such as ethanol at a range of temperatures between room temperature and 70°C and pressures between atmospheric and 4 Barr.
  • Compounds of formula (9a) may be prepared from compounds of general formula (6a) by means of a reaction such as a Wittig (or one of the closely related variants such as the Horner- Wadsworth-Emmons) with a suitable substrate such as R 1 -CH 2 -P(0)(OMe) 2 in the presence of a suitable base such as sodium hydride in a suitable solvent such as tetrahydrofuran at a range of temperatures preferably between -10°C and 100°C.
  • a reaction such as a Wittig (or one of the closely related variants such as the Horner- Wadsworth-Emmons) with a suitable substrate such as R 1 -CH 2 -P(0)(OMe) 2 in the presence of a suitable base such as sodium hydride in a suitable solvent such as tetrahydrofuran at a range of temperatures preferably between -10°C and 100°C.
  • R 1 -CH 2 -P(0)(OMe) 2 may be synthesised from compounds of the general formula R 1 -CH 2 -Hal wherein Hal represents a halogen such as -Br or -CI by reaction with a compound such as trimethylphosphite at a range of temperatures preferably between 0°C and 100°C.
  • R 1 -CH 2 -Hal may be synthesised from compounds of formula R'-CHs by means of a reaction such as a radial halogenation using a reagent such as N-bromosuccinimide in the presence of a catalyst such as AIBN in a suitable solvent such as carbon tetrachloride at a range of temperatures preferably between 0°C and 80°C.
  • a reaction such as a radial halogenation using a reagent such as N-bromosuccinimide in the presence of a catalyst such as AIBN in a suitable solvent such as carbon tetrachloride at a range of temperatures preferably between 0°C and 80°C.
  • R 1 -CH 2 -Hal may also be synthesised from compounds formula R 1 -CH 2 -OH by means of using halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane or activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropylamine.
  • halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane
  • activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropylamine.
  • R'-CHs and R 1 -CH 2 -OH may be prepared by methods outlined above for compounds (2b), (2c) and (2d).
  • Compounds of formula (lac) may be prepared from compounds of formula (10c) wherein PG is a suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using suitable de-protection conditions such as, sodium hydroxide in methanol, trifluoroacetic acid in dichloromethane or hydrogen gas catalysed by for example palladium on carbon in ethanol, at a range of temperatures, preferably between 0°C and 100°C.
  • suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using suitable de-protection conditions such as, sodium hydroxide in methanol, trifluoroacetic acid in dichloromethane or hydrogen gas catalysed by for example palladium on carbon in ethanol, at a range of temperatures, preferably between 0°C and 100°C.
  • Compounds of formula (10c) may be prepared from compounds of formula (10b) wherein PG is a suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using hydrogen gas in the presence of a catalyst such as palladium on carbon, in a suitable solvent such as methanol or ethanol, in the presence or absence of an acid such as HC1, at a range of temperatures, preferably between 0°C and 100°C.
  • PG is a suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using hydrogen gas in the presence of a catalyst such as palladium on carbon, in a suitable solvent such as methanol or ethanol, in the presence or absence of an acid such as HC1, at a range of temperatures, preferably between 0°C and 100°C.
  • Compounds of formula (10b) may be prepared from compounds of formula (10a) and (lOf) by a reaction such as a cross-coupling using a suitable catalyst such as tetrakis(triphenylphosphine)palladium (0) or palladium acetate, and a base such as diisopropylethylamine, sodium tert-butoxide or caesium carbonate in a suitable solvent such as NMP, toluene or DMF, at a range of temperatures, preferably between 0°C and 100°C.
  • a suitable catalyst such as tetrakis(triphenylphosphine)palladium (0) or palladium acetate
  • a base such as diisopropylethylamine, sodium tert-butoxide or caesium carbonate
  • a suitable solvent such as NMP, toluene or DMF
  • Compounds of formula (10a) may be prepared from compounds of formula (6a) using a inflating agent such as triflic anhydride, in the presence of a suitable base such as pyridine or 2,6-bis(tert-butyl)-4-methylpyridine, in a solvent such as dichloromethane or chloroform at a range of temperatures, preferably between 0°C and boiling point of the solvent.
  • a inflating agent such as triflic anhydride
  • a suitable base such as pyridine or 2,6-bis(tert-butyl)-4-methylpyridine
  • a solvent such as dichloromethane or chloroform
  • 'flash silica' refers to silica gel for chromatography, 0.035 to 0.070 mm (220 to 440 mesh) (e.g. Fluka silica gel 60), and an applied pressure of nitrogen up to 10 p.s.i accelerated column elution or use of the CombiFlash® Companion purification system or use of the Biotage SP l purification system. All solvents and commercial reagents were used as received.
  • MS ionization method Electrospray (positive and negative ion).
  • MS ionization method Electrospray (positive and negative ion).
  • MS ionization method Electrospray (positive and negative ion).
  • MS ionization method Electrospray (positive and negative ion).
  • MS ionization method Electrospray (positive and negative ion).
  • Agilent 1260 infinity purification system Column: XSELECT CSH Prep C 18 OBD, particle size 5 ⁇ , 30 x 150mm, RT. Elution with A: water + 0.1% formic acid; B: CH 3 CN + 0.1% formic acid. Gradient - 90% A/10% B to 2% A/95% B over 22 min - flow rate 60 mL/min. Detection - In-line Agilent 6100 series single Quadrupole LC/MS.
  • Agilent 1260 infinity purification system Column: XBridge Prep C 18 OBD, particle size 5 ⁇ , 30 x 150mm, RT. Elution with A: water + 0.1% ammonia; B: CH 3 CN + 0.1% ammonia. Gradient - 90% A/10% B to 2% A/95% B over 22 min - flow rate 60 mL/min. Detection - In-line Agilent 6100 series single Quadrupole LC/MS.
  • Diastereomeric purity was measured using Marfey's method; compound 2 (1 mg, 3.68 ⁇ ) was dissolved in EtOAc (1 mL) and H 2 0 (1 mL) and Marfeys reagent was added (N a -(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, FDAA [CAS 95713-52-3] , 1 mg, 3.68 ⁇ ) followed by saturated NaHC0 3 solution (50 ⁇ ) and heated to 50°C for 1 hour.
  • Racemic 2-methylpiperidine was also subjected to Marfey's method; HPLC : Rt 10.75 min (50%), 1 1 .581 min (50%).
  • Tetrabutylammonium fluoride (1M solution in THF, 21 1 ⁇ ⁇ , 0.21 1 mmol) was added to a solution of Intermediate 14f (1 10 mg, 0.151 mmol) in THF (3 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (2 x 5 mL) and brine (5 mL). The organic layer was passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to afford the title compound (52 mg, 60%). LCMS (Method 3): Rt: 2.91 min, m/z 573 [MH + ].
  • Example 14 step g to give the title compound.
  • Tetrabutylammonium fluoride (1M solution in THF, 0.211 mmol) was added to a solution of the appropriate Triisopropylsilanyloxymethyl-piperidin-intermediate (Ex. 30-37) (0.151 mmol) in THF (3 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (2 x 5 mL) and brine (5 mL). The organic layer was passed through a phase separator and concentrated in vacuo.
  • OZda/X3d 00t7S6l/M0Z OAV (ds-DMSO): 1.21 (9H, s), 1.73-1.85 (2H, m), 1.85-

Abstract

This invention relates to compounds and compositions that are p38 MAPK inhibitors, useful as anti-inflammatory agents in the treatment of, inter alia, diseases of the respiratory tract.

Description

KINASE INHIBITORS
This invention relates to compounds and compositions that are p38 MAPK inhibitors, useful as anti-inflammatory agents in the treatment of, inter alia, diseases of the respiratory tract.
Background to the invention
Mitogen activated protein kinases (MAPK) constitute a family of proline- directed serine/threonine kinases that activate their substrates by dual phosphorylation. There are four known human iso forms of p38 MAP kinase, p38a, ρ38β, ρ38γ and ρ38δ. The p38 kinases, which are also known as cytokine suppressive anti-inflammatory drug binding proteins (CSBP), stress activated protein kinases (SAPK) and RK, are responsible for phosphorylating (Stein et al., Ann. Rep. Med Chem., 1996, 31 , 289-298) and activating transcription factors (such as ATF-2, MAX, CHOP and C/ERPb) as well as other kinases (such as MAPKAP- K2/3 or MK2/3), and are themselves activated by physical and chemical stress (e.g. UV, osmotic stress), pro-inflammatory cytokines and bacterial lipopolysaccharide (LPS) (Herlaar E. & Brown Z., Molecular Medicine Today, 1999, 5, 439-447). The products of p38 phosphorylation have been shown to mediate the production of inflammatory cytokines, including tumor necrosis factor alpha (TNF a) and interleukin- (IL)-l , and cyclooxygenase-2 (COX-2). IL-1 and TNFa are also known to stimulate the production of other proinflammatory cytokines such as IL-6 and IL- 8.
IL-1 and TNFa are biological substances produced by a variety of cells, such as monocytes or macrophages. IL-1 has been demonstrated to mediate a variety of biological activities thought to be important in immunoregulation and other physiological conditions such as inflammation (e.g. Dinarello et al., Rev. Infect. Disease, 1984, 6, 51). Excessive or unregulated TNF production (particularly TNFa) has been implicated in mediating or exacerbating a number of diseases, and it is believed that TNF can cause or contribute to the effects of inflammation in general. IL-8 is a chemotactic factor produced by several cell types including mononuclear cells, fibroblasts, endothelial cells, and keratinocytes. Its production from endothelial cells is induced by IL-1 , TNF, or lipopolysaccharide (LPS). IL-8 stimulates a number of functions in vitro. It has been shown to have chemoattractant properties for neutrophils, T-lymphocytes and basophils. Increase in IL-8 production is also responsible for chemotaxis of neutrophils into the inflammatory site in vivo.
Inhibition of signal transduction via p38, which in addition to IL-1 , TNF and IL-8 described above is also required for the synthesis and/or action of several additional pro-inflammatory proteins (e.g., IL-6, GM-CSF, COX-2, collagenase and stromelysin), is expected to be a highly effective mechanism for regulating the excessive and destructive activation of the immune system. This expectation is supported by the potent and diverse anti- inflammatory activities described for p38 kinase inhibitors (Badger et al , J. Pharm. Exp. Thera., 1996, 279, 1453 -1461 ; Griswold et al, Pharmacol. Comm. ,1996, 7, 323-229). In particular, p38 kinase inhibitors have been described as potential agents for treating rheumatoid arthritis. In addition to the links between p38 activation and chronic inflammation and arthritis, there is also data implicating a role for p38 in the pathogenesis of airway diseases in particular COPD and asthma. Stress stimuli (including tobacco smoke, infections or oxidative products) can cause inflammation within the lung environment. Inhibitors of p38 have been shown to inhibit LPS and ovalbumin induced airway TNF-a, IL-Ι β, IL-6, IL-4, IL-5 and IL-13 (Haddad et al, Br. J. Pharmacol, 2001 , 132 (8), 1715-1724; Underwood et al, Am. J. Physiol. Lung Cell. Mol. 2000, 279, 895-902; Duan et al , 2005 Am. J. Respir. Crit. Care Med., 171 , 571-578; Escott et al Br. J. Pharmacol, 2000, 131 , 173-176; Underwood et al , J. Pharmacol. Exp. Ther. 2000, 293, 281-288). Furthermore, they significantly inhibit neutrophilia and the release of MMP-9 in LPS, ozone or cigarette smoke animal models. There is also a significant body of preclinical data highlighting the potential benefits of inhibition of the p38 kinase that could be relevant in the lung (Lee et al., Immunopharmacology, 2000, 47, 185-200). Thus, therapeutic inhibition of p38 activation may be important in the regulation of airway inflammation.
The implication of the p38MAPK pathway in various diseases has been reviewed by P. Chopra et al. (Expert Opinion on Investigational Drugs, 2008, 17(10), 141 1-1425). It is believed that the compounds of the present invention can be used to treat p38 mediated diseases such as: chronic obstructive pulmonary disease (COPD), asthma, chronic or acute broncho constriction, bronchitis, acute lung injury and bronchiectasis, pulmonary artery hypertension, tuberculosis, lung cancer, inflammation generally (e.g. inflammatory bowel disease), arthritis, neuroinflammation, pain, fever, fibrotic diseases, pulmonary disorders and diseases (e.g., hyperoxic alveolar injury), cardiovascular diseases, post -ischemic reperfusion injury and congestive heart failure, cardiomyopathy, stroke, ischemia, reperfusion injury, renal reperfusion injury, brain edema, neurotrauma and brain trauma, neurodegenerative disorders, central nervous system disorders, liver disease and nephritis, gastrointestinal conditions, ulcerative diseases, Crohn's disease, ophthalmic diseases, ophthalmo logical conditions, glaucoma, acute injury to the eye tissue and ocular traumas, diabetes, diabetic nephropathy, skin-related conditions, myalgias due to infection, influenza, endotoxic shock, toxic shock syndrome, autoimmune disease, graft rejection, bone resorption diseases, multiple sclerosis, psoriasis, eczema, disorders of the female reproductive system, pathological (but non -malignant) conditions, such as hemangiomas, angiofibroma of the nasopharynx, and avascular necrosis of bone, benign and malignant tumors/neoplasia including cancer, leukaemia, lymphoma, systemic lupus erythematosus (SLE), angiogenesis including neoplasia, haemorrhage, coagulation, radiation damage, and/or metastasis. Chronic release of active TNF can cause cachexia and anorexia, and TNF can be lethal. TNF has also been implicated in infectious diseases. These include, for example, malaria, mycobacterial infection and meningitis. These also include viral infections, such as HIV, influenza virus, and herpes virus, including herpes simplex virus type-1 (HSV-1), herpes simplex virus type-2 (HSV-2), cytomegalovirus (CMV), varicella-zoster virus (VZV), Epstein-Barr virus, human herpes virus-6 (HHV-6), human herpesvirus-7 (HHV7), human herpesvirus-8 (HHV-8), pseudorabies and rhino tracheitis, among others.
Known P38 kinase inhibitors have been reviewed by G. J. Hanson (Expert Opinions on Therapeutic Patents, 1997, 7, 729-733) J Hynes et al. (Current Topics in Medicinal Chemistry, 2005, 5, 967-985), C. Dominguez et al (Expert Opinions on Therapeutics Patents, 2005, 15, 801-816) and L. H. Pettus & R. P. Wurtz (Current Topics in Medicinal Chemistry, 2008, 8, 1452-1467). P38 kinase inhibitors containing a triazolopyridine motif are known in the art, for example WO07/091 152, WO04/072072, WO06/018727.
International Patent Application WO2010/094956 discloses triazolopyridine derivatives of formula I) as being p38 MAP Kinase inhibitors:
Figure imgf000005_0001
In such compounds, A represents an optionally substituted divalent arylene radical, an heteroarylene radical, a (C5-C6) divalent cycloalkylene radical having 5 or 6 ring atoms or a pyperidinylene radical.
Other p38 MAP Kinase inhibitors are described in the co-pending applications PCT/EP201 1/072375, PCT/EP2012/074446 and PCT/EP2012/074450.
The compounds are said to be useful in as anti- inflammatory agents in the treatment of diseases of the respiratory tract.
The object of the present invention is to identify novel and potent p38 mitogen activated protein kinase inhibitors which are useful in the treatment of inflammatory and obstructive diseases of the respiratory tract. It is another object of the present invention, to identify novel potent p38 mitogen activated protein kinase inhibitors which show an appropriate developability profile on inhalatory administration to effectively treat respiratory obstructive or inflammatory diseases. It is to be understood that such profile may be achieved in a number of different ways by modulation of specific properties; by way of example, it could be achieved by administration of a low effective dose of the drug thus limiting side effects or via a long duration of action in the lungs which may reduce the frequency of administration.
Brief Description of the Invention
The compounds of the present invention are inhibitors of p38 mitogen activated protein kinase ("p38 MAPK", "p38 kinase" or "p38"), including p38a kinase, and are inhibitors of cytokine and chemokine production including TNFa and IL-8 production. They have a number of therapeutic applications, in the treatment of inflammatory diseases, particularly allergic and non-allergic airways diseases, more particularly obstructive or inflammatory airways diseases such as chronic obstructive pulmonary disease ("COPD") and asthma. They are therefore particularly suited for pulmonary delivery, by inhalation by nose or mouth.
Description of the invention
According to the invention there is provided a compound of formula (I), or a pharmaceutically acceptable salt thereof:
Figure imgf000006_0001
(I)
wherein:
W is a heteroatom selected from N or O, wherein N is substituted with -H, Ci- C6 alkyl or C3-C5 cycloalkyl;
Y is selected in the group consisting of: a group -S(0)p- wherein p is 0, 1 or 2; a group -0(CR3R4)n-; a group -(CR5R6)„-; a group -NR7-; a group -OC(O)-; a group -OC(0)NH-; and a group -OC(0)0-;
n is 0, 1 , 2 or 3;
R3, R4, R5 and R6 are each independently -H, fluorine or Ci-C6 alkyl, or, respectively, R3 and R4, or R5 and R6 may form together with the carbon atom to which they are attached a saturated 3-6 membered carbocyclic monocyclic ring optionally substituted by a group Ci-C6 alkyl, hydroxyl or halo;
R7 is -H, Ci-C6 alkyl, or C3-C7 cycloalkyl wherein such Ci-C6 alkyl or C3-C7 cycloalkyl are optionally substituted by a group C1-C3 alkyl, C3-C6 cycloalkyl, hydroxyl, -CN or halo;
R1 is a group (Ila)
R1
/
N
X
R (Ila)
X2, X3, X4 and Xs are each independently a carbon atom, a nitrogen atom, a group -(CH)- or a group -NH-; such that each combination thereof forms an aromatic ring system;
R11 is linked to X4 and is selected from a group consisting of:
-NRARB, -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)- NRARB, -(Ci-C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C2- C6alkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)-NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)- (C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)- (C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3- C7cycloalkylene)-ORD, -N(Rc)C(0)N(RARB), -C(0)N(RARB), -N(Rc)C(0)N(Rc)- (C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C2- C6alkylene)-ORD, -0-(C3-C7cycloalkylene)-ORD, -S-(C2-C6alkylene)-ORD, -S-(C3- C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-ORD, -S(0)2N(Rc)-(C3- C7cycloalkylene)-ORD, -N(Rc)S(0)2-(C2-C6alkylene)-ORD, -N(Rc)S(0)2(C3- C7cycloalkylene)-ORD, -S(0)2N(RARB), -N(Rc)S(0)2RD, -N(Rc)C(0)Rc, ORc, SRC, -(C3-C7heterocycloalkyl), (C5-C7heterocycloalkyl)-(Ci-C6alkyl), (C5-
C7heterocycloalkyl)(C3-C6cycloalkyl) and (C3-C7 heterocycloalkyl)carbonyl, wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)- -(C2- C6alkylene)-, -(C3-C7cycloalkylene)-, -(C3-C7heterocycloalkyl) and (C5- C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxy 1 and halo; or
R11 is linked to X4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is optionally substituted by one or more group(s) independently selected from: halo, ORD, -CN, -S02RD, Ci-C6 alkyl which is substituted by one or more groups -CN; C3-C6 cycloalkyl which is substituted by a group selected from: -CN, -ORc, -SRC or halo ; -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-NRARB, -(Ci- C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)- NRARB, -S-(C2-C6alkylene)-NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)- (Ci-C6alkylene)-NRARB, -N(Rc)C(0)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)- (C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)- (C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)C(0)N(Rc)- (C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C3- C7cycloalkylene)-ORD, -S-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)- NRARB, -N(Rc)S(0)2-(C3-C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)- NRARB, -S(0)2N(Rc)-(C3-C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)- ORD, -S(0)2N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(C2-C6alkylene)-ORD, -N(Rc)S(0)2-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2RD, -(C3-C7heterocycloalkyl), (C5-C7heterocycloalkyl)-(Ci_C6alkyl), (C5-C7heterocycloalkyl)(C3-C6cycloalkyl) and (C3-C7heterocycloalkyl)carbonyl, wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)- -(C2-Cealkylene)-, -(C3-C7cycloalkylene)-, -(C3- C7heterocycloalkyl) and -(C5-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the group consisting of: Ci-C6alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7cycloalkyl, hydroxyl, -CN and halo;
RA and RB are at each occurrence independently -H, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7 cycloalkyl, -ORD, -CN, -NRA RB' or halo; alternatively, RA and RB, may form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more groups independently selected from -ORD, -CN, -NRA RB', halo, Ci-C6 alkyl, -(C3-C7) cycloalkyl-NRA RB', -(Ci-C5 alkylene)-NRA'RB', -0-(Ci-C5 alkylene)-NRA RB' and -0-(C3-C7cycloalkyl)- NRA'RB',-N(Rc')C(0)-(Ci-C6alkylene)-NRA'RB', -N(Rc')C(0)-(C3-C7cycloalkylene)- NRA RB , -C(0)N(Rc')-(C2-C6alkylene)-NRA'RB', -C(0)N(Rc')-(C3-
C7cycloalkylene)-NRA'RB' such Ci-C6 alkyl, C2-C5 alkyl and C3-C7 cycloalkyl portions being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD, -CN, -NRA RB or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3- C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD, -CN, or halo; or RA and RB may be linked to one carbon atom of the -(Ci-Cealkylene)- or -(C3- C7cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms; Rc is at each occurrence independently -H, Ci-C6 alkyl, (Ci-C4)hydroxy alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl, (Ci-C4)hydroxyalkyl and C3-C6 cycloalkyl being optionally substituted by a group C1-C3 alkyl, -ORD, -CN or halo;
alternatively Rc may be linked to one carbon atom of the -(C2-C6alkylene)- or -(C3-Cycycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
RD is at each occurrence independently -H, -CH3, -C2H5 or (Ci- C4)hydroxyalkyl;
RA and RB are at each occurrence independently -H, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1-C3 alkyl, C3-C7 cycloalkyl, -ORD , -CN or halo; alternatively, RA and RB , may form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -ORD, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1-C3 alkyl, C3-C7cycloalkyl, -ORD , -CN or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, - ORD', -CN, or halo; or RA' and RB' may be linked to one carbon atom of the -(Ci- C6alkylene)- or -(C3-C7Cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
RD is at each occurrence independently -H, -CH3 or -C2H5;
Rc is at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl and C3-C6 cycloalkyl being optionally substituted by a group C1-C3 alkyl, -ORD', -CN or halo;
R13 are independently -H, Ci-C6 alkyl or halo; A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms; said cycloalkylene ring being attached to W and Y, and optionally fused to a phenyl ring or to a monocyclic heteroaryl ring having 5 or 6 ring atoms, such phenyl or heteroaryl ring being optionally substituted by one or two groups R24;
R24 is at each occurrence independently selected from the group consisting of: Ci-Ce alkyl, halo and CN;
R2 is a radical of formula
Figure imgf000011_0001
K (Ilia)
R17 is a group of general formula (IV):
Figure imgf000011_0002
R19 is selected in a group consisting of: -H, -CH3, CN and OH; R20 is selected in the group consisting of: -H, -F, -CH3, -C2H5, -CH2OH, -CH2OMe, -CF2CF3, -CH2SCH3, -SCH3 and -SC2H5; R21 is -H, -CH3 or -C2H5;
R18 is absent or is selected in the group consisting of aryl, heteroaryl, -(Ci-C6alkyl), -(C3-C7cycloalkyl), -(C3-Cyheterocycloalkyl), (C5-Cvheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C6alkyl), -(C3-Cycycloalkyl), -(C3-Cyheterocycloalkyl), (C5- Cyheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl) may be optionally substituted by one or more group(s) selected from -CN, -OH, =0, halo, -COORM, Ci-C6alkyl, C3-C6cycloalkyl, -0-(Ci-C6alkyl), -0-(C3-C6cycloalkyl), -S02RM; -S-(Ci-C6alkyl), -S-(C3-C6cycloalkyl), -NRHRJ, -N(RL)(C2-C6alkylene)- NRHRJ, -N(RL)(C3-C7cycloalkylene)-NRHRJ, -(Ci-C6alkylene)-NRHRJ, -(C3- C7cycloalkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ, -0-(C3-C7cycloalkylene)- NRHRJ, -S-(C2-C6alkylene)-NRHRJ, -S-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)- (Ci-C6alkylene)-NRHRJ, -N(RL)C(0)-(C3-C7cycloalkylene)-NRHRJ, -C(0)N(RL)- (C2-C6alkylene)-NRHRJ, -C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)N(RHRJ), -C(0)N(RHRJ), -N(RL)C(0)N(RL)-(C2-C6alkylene)-NRHRJ, -N(RL)C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)S(0)2-(Ci-C6alkylene)- NRHRJ, -N(RL)S(0)2-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RL)-(C2-C6alkylene)- NRHRJ, -S(0)2N(RL)-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RHRJ), ORL, SRL wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)-, -(C2- C6alkylene)-, -(C3-C7cycloalkylene)-, -(C3-C7heterocycloalkyl) and (C5- C7heterocycloalkyl) portion in the above listed groups, may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo;
RH and RJ, are at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, -CN, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORM, -CN or halo; and which 6- 1 1 - membered saturated monocyclic or bicyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORM, -CN, or halo;
RL is at each occurrence independently -H, Ci -C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo;
RM is at each occurrence independently -H, Ci -C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group hydroxyl, -CN or halo;
R° and RP are each independently Ci-C6 alkyl or Ci-C6 cycloalkyl, optionally substituted by a group C1-C3 alkyl, -ORQ, -CN or halo; alternatively, R° and RP may form together with the nitrogen atom to which they are attached a 4-8- membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORQ, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, and which 6-8- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl;
RQ is -H, -CH3 or -C2H5;
z1, z2, z3, and z4 are independently selected in the group consisting of: C, N, S, O, a group -CH-, and a group -NH-, in such a combination that the resulting ring formed is an aromatic system;
with the proviso that when there is any combination among any of the substituents of R19 selected in a group consisting of -CH3 and R20 selected in the group consisting of -F, -CH3, -C2H5, -CH2OH, -CH2OMe, -CF2CF3, -CH2SCH3, -SCH3 and -SC2H5 and R21 selected from -CH3 and -C2H5;
and
A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms fused to a phenyl ring or monocyclic heteroaryl,
then
R11 is -NRARB, -N(RC)(C2-C6alkylene)-NRARB, -N(RC)(C3- C7cycloalkylene)-NRARB, -(Ci-C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C2-C6alkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)- NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)N(RARB), -C(0)N(RARB), -N(Rc)C(0)N(Rc)-(C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3- C7cycloalkylene)-NRARB, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB, -S(0)2N(RARB), wherein any of such -(Ci-C6alkylene)- -(C2-C6alkylene)-, -(C3-C7cycloalkylene)- and -(C3-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl,
(Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein
RA and RB are at each occurrence independently form together with the nitrogen atom to which they are attached a azetidine or a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is substituted by -NRA RB , - (Ci-C5alkylene)-NRA'RB', -0-(Ci-C5alkyl)-NRA RB', -0-(C3-C7cycloalkyl)-NRA RB', -N(Rc')C(0)-(Ci-C6alkylene)-NRA'RB', -N(Rc')C(0)-(C3-C7cycloalkylene)-NRA RB', -C(0)N(Rc')-(C2-C6alkylene)-NRA'RB', -C(0)N(Rc')-(C3-C7cycloalkylene)-NRA RB ' and optionally by one or more group selected from -ORD, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD, -CN, -NRA RB' or halo; and which 6- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD, -CN, or halo;
or R11 is linked to X4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is substituted by two or more group(s) selected from: -N(RC)(C2-Cealkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-NRARB, -(Ci-C6alkylene)-NRARB, -(C3-
C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)- NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)C(0)N(Rc)-(C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)-ORD, -S- (C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB; and additionally optionally substituted by one or more group(s) selected from: Ci-C6 alkyl, C3-C6 cycloalkyl, halo, -ORD, -CN, S02RD, -CONRARB wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)-, -(C2-C6alkylene)-, -(C3-C7cycloalkylene)- and -(C3-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the group consisting of: Ci-C6alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7cycloalkyl, hydroxyl, CN and halo;
wherein RA and RB are at each occurrence independently hydrogen, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD', -CN, -NRA RB' or halo; alternatively, RA and RB, may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -ORD , -CN, -NRA RB , halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci -C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD , -CN or halo; and which 6- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD , -CN, or halo; and/or RA and RB may be linked to one carbon atom of the -(Ci-Cealkylene)-, -(C2-Cealkylene)- or -(C3-C7cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
or
- R18 is selected in the group consisting of aryl, heteroaryl, -(Ci-C6alkyl), -(C3-C7cycloalkyl), -(C3-C7heterocycloalkyl), (C5-Cvheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C6alkyl),-(C3-Cycycloalkyl), -(C3-Cyheterocycloalkyl), (C5- Cyheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl) is substituted by a group selected from NRHRJ, -N(RL)(C2-C6alkylene)-NRHRJ, -N(RL)(C3-C7cycloalkylene)-NRHRJ, -(Ci-C6alkylene)-NRHRJ, -(C3- C7cycloalkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ, -0-(C3-C7cycloalkylene)- NRHRJ, -S-(C2-C6alkylene)-NRHRJ, -S-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)- (Ci-C6alkylene)-NRHRJ, -N(RL)C(0)-(C3-C7cycloalkylene)-NRHRJ, -C(0)N(RL)- (C2-C6alkylene)-NRHRJ, -C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)N(RHRJ), -C(0)N(RHRJ), -N(RL)C(0)N(RL)-(C2-C6alkylene)-NRHRJ, -N(RL)C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)S(0)2-(Ci-C6alkylene)- NRHRJ, -N(RL)S(0)2-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RL)-(C2-C6alkylene)- NRHRJ, -S(0)2N(RL)-(C3-C7cycloalkylene)-NRHRJ, and wherein any portion in the above listed groups may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo; and wherein RH and RJ at each occurrence independently form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is substituted by NR°RP and optionally by one or more additional groups -ORM, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1 -C3 alkyl, C3-C7cycloalkyl, -ORM, CN or halo; and which 6-1 1- membered saturated monocyclic or bicyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORM, CN, or halo;
wherein RA', RB', Rc, Rc', RD, RD', RH, RJ, RL, RM, R° and RP are as defined above.
In another aspect, the invention includes pharmaceutical compositions comprising a compound of the invention, together with one or more pharmaceutically acceptable carriers and/or excipients. Particularly preferred are compositions adapted for inhalation for pulmonary administration.
In another aspect, the invention includes the use of a compound of the invention for the treatment of diseases or conditions which benefit from inhibition of p38 MAP kinase activity. The treatment of obstructive or inflammatory airways diseases is a preferred use. All forms of obstructive or inflammatory airways diseases are potentially treatable with the compounds of the present invention, in particular an obstructive or inflammatory airways disease that is a member selected from the group consisting of chronic eosinophilic pneumonia, asthma, COPD, COPD that includes chronic bronchitis, pulmonary emphysema or dyspnea associated or not associated with COPD, COPD that is characterized by irreversible, progressive airways obstruction, adult respiratory distress syndrome (ARDS), exacerbation of airways hyper-reactivity consequent to other drug therapy and airways disease that is associated with pulmonary hypertension, chronic inflammatory diseases including cystic fibrosis, bronchiectasis and pulmonary fibrosis (Idiopathic). Efficacy is anticipated when p38 kinase inhibitors are administered either locally to the lung (for example by inhalation and intranasal delivery) or via systemic routes (for example, oral, intravenous and subcutaneous delivery).
Terminology
As used herein, the terms "halogen" or "halo" include fluorine, chlorine, bromine and iodine atoms.
As used herein, the term "Cx-Cyalkyl" wherein x and y are integers, refers to a straight or branched chain alkyl radical having from x to y carbon atoms. Thus when χ is 1 and y is 6, for example, the term includes methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl.
As used herein, the term "Cx-Cyhaloalkyl" refers to the above "Cx-Cyalkyl" group wherein one or more hydrogen atoms are replaced by one or more halogen atoms.
As used herein, the term "Cx-Cyhydroxyalkyl" refers to the above "Cx- Cyalkyl" group wherein one hydrogen atom is replaced by one hydroxyl group.
As used herein, the term "Cx-Cyalkylene" wherein x and y are integers, refers to a Cx-Cyalkyl radical having in total two unsatisfied valencies, such as a divalent methylene radical.
As used herein, the term "carbocyclic" refers to a mono-, bi- or tricyclic radical having up to 16 ring atoms, all of which are carbon, and includes aryl and cycloalkyl.
As used herein, the term "Cz-CkCycloalkyl" wherein z and k are integers refers to a monocyclic saturated carbocyclic radical having from z to k carbon atoms and includes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. Comprised within the scope of the term "Cz-CkCycloalkyl", are those radicals having two unsatisfied valencies on the same carbon atom which will link to any Cx-Cyalkyl, Cx-Cyalkylene Cz-CkCycloalkyl Cz-CkCycloalkylene, Cz- Ckheterocycloalkyl, Cz-CkheterocycloalkylCx-Cyalkyl, Cz-CkheterocycloalkylCz- CkCycloalkyl or (Cz-Ck)heterocycloalkylcarbonyl group by replacement of two hydrogen atoms placed on the same carbon. In such circumstances, this radical forms a g em- di substituted or spiro system together with the Cx-Cyalkyl, Cx- Cyalkylene Cz-CkCycloalkyl Cz-CkCycloalkylene, Cz- Ckheterocycloalkyl, Cz- CkheterocycloalkylCx-Cyalkyl, Cz-CkheterocycloalkylCz-CkCycloalkyl or (Cz- Ck)heterocycloalkylcarbonyl group it is linked to.
The term "Cz-CkCycloalkylene radical" refers to a Cz-CkCycloalkyl radical having two unsatisfied valencies on different cycle carbon atoms as follows: *
Figure imgf000019_0001
As used herein, the unqualified term "aryl" refers to a mono- or bi-cyclic carbocyclic aromatic radical, and includes radicals having two monocyclic carbocyclic aromatic rings which are directly linked by a covalent bond. Illustrative of such radicals are phenyl, biphenyl and naphthyl.
As used herein, the unqualified term "heteroaryl" refers to a mono- or bicyclic aromatic radical containing one or more heteroatoms selected from S, N and O, and includes radicals having two such monocyclic rings, or one such monocyclic ring and one monocyclic aryl ring, which are fused through a common bond. Illustrative examples of 5,6-membered heteroaryl are: are thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, isothiazolyl, pyrazolyl, oxazolyl, isoxazolyl, isothiazolyl, triazolyl, thiadiazolyl, oxadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, and triazinyl. Illustrative examples of 8, 10-membered heteroaryl are: benzothienyl, benzo furyl, benzimidazolyl, benzothiazolyl, benzisothiazolyl, benzoxazolyl, benzisoxazolyl, benzotriazolyl, indolyl and indazolyl.
As used herein, the unqualified term "heterocyclyl" or "heterocyclic" and relates to a saturated mono-, bi- or tri-cyclic non-aromatic radical containing one or more heteroatoms selected from S, N and O. In the case of bicyclic heterocyclic systems, included within the scope of the term are fused, spiro and bridged bicyclic systems. In particular, the term "Cz-Ckheterocycloalkyl" refers to monocyclic (Cz- Ck)cycloalkyl groups, in which at least one ring carbon atom is replaced by a heteroatom (e.g. N, NH, S or O). Examples of (Cz-Ck)heterocycloalkyl include pyridonyl, pyrrolidinyl, thiazolidinyl, piperazinyl, piperidinyl, morpholinyl, thio morpho liny 1.
By analogy, the term "Cz-Ckheterocycloalkylene", refers to a divalent Cz-
Ckheterocycloalkyl radical, wherein Cz-Ckheterocycloalkyl is as above defined. The term "Cz-CkheterocycloalkylCx-Cyalkyl" refers to the above "Cx-Cyalkyl" group wherein one or more hydrogen atoms are replaced by one or more "Cz- Ckheterocycloalkyl" groups. Comprised within the scope of the term "Cz- CkheterocycloalkylCx-Cyalkyl" are systems where two hydrogen atoms linked to the same carbon atom in "Cx-Cyalkyl" group are replaced by one "Cz- Ckheterocycloalkyl" group. Such radical thus form a gem-disubstituted "Cz- CkheterocycloalkylCx-Cyalkyl" system, such as a 1 ,2-dimethyl-pyrrolidin-2-yl radical.
The term "Cz-CkheterocycloalkylCz-CkCycloalkyl" refers to the above "Cz- CkCycloalkyl" group wherein one or more hydrogen atoms are replaced by one or more "Cz-Ckheterocycloalkyl" groups.
The expression "(Cz-Ck)cycloalkylcarbonyl" refers to (Cz-Ck)cycloalkyl-CO- groups wherein the group "(Cz-Ck)cycloalkyl" has the meaning above defined.
The expression "(Cz-Ck)heterocycloalkylcarbonyl" refers to (Cz- Ck)heterocycloalkyl-CO- groups wherein the group "(Cz-Ck)heterocycloalkyl" has the meaning above defined.
Compounds of the invention may exist in one or more geometrical, optical, enantiomeric, diastereomeric and tautomeric forms, including but not limited to cis- and trans-forms, E- and Z-forms, R-, S- and meso-forms. Unless otherwise stated a reference to a particular compound includes all such isomeric forms, including racemic and other mixtures thereof. Where appropriate such isomers can be separated from their mixtures by the application or adaptation of known methods (e.g. chromatographic techniques and recrystallisation techniques). Where appropriate such isomers may be prepared by the application of adaptation of known methods (e.g. asymmetric synthesis).
Throughout the specification the use of an asterisk "*" in the definition of a structural formula, indicates the point of attachment for the radical group to the rest of the molecule. As used herein the term "salt" includes base addition, acid addition and ammonium salts. As briefly mentioned above compounds of the invention which are acidic can form salts, including pharmaceutically acceptable salts, with bases such as alkaline metal hydroxides, e.g. sodium and potassium hydroxides; alkaline earth metal hydroxides e.g. calcium, barium and magnesium hydroxides; with organic bases e.g. N-methyl-D-glucamine, choline tris(hydroxymethyl)amino-methane, L- arginine, Z-lysine, N-ethyl piperidine, dibenzylamine and the like. Those compounds of the invention which are basic can form salts, including pharmaceutically acceptable salts with inorganic acids, e.g. with hydrohalic acids such as hydrochloric or hydrobromic acids, sulphuric acid, nitric acid or phosphoric acid and the like, and with organic acids e.g. with acetic, formic, trifluoroacetic, tartaric, succinic, fumaric, maleic, malic, salicylic, citric, methanesulphonic, p-toluenesulphonic, benzoic, benzenesulfonic, glutamic, lactic, and mandelic acids and the like. Those compounds (I) which have a basic nitrogen can also form quaternary ammonium salts with a pharmaceutically acceptable counter-ion such as ammonium, chloride, bromide, acetate, formate, p-toluenesulfonate, succinate, hemi- succinate, naphthalene-bis sulfonate, methanesulfonate, trifluoroacetate, xinafoate, and the like. For a review on salts, see Handbook of Pharmaceutical Salts: Properties, Selection, and Use by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).
It is expected that compounds of the invention may be prepared in the form of hydrates, and solvates. Any reference herein, including the claims herein, to "compounds with which the invention is concerned" or "compounds of the invention" or "the present compounds", and the like, includes reference to salts hydrates, and solvates of such compounds. The term 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and a stoichiometric amount of one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term 'hydrate' is employed when said solvent is water. Individual compounds of the invention may exist in several polymorphic forms and may be obtained in different crystal or co-crystal habits, and they are intended to be included within the meaning of the term "compounds of the invention".
The compounds may also be administered in the form of prodrugs thereof.
Thus certain derivatives of the compounds which may be active in their own right or may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of the invention having the desired activity, for example, by hydrolytic cleavage. Such derivatives are referred to as 'prodrugs' . Further information on the use of prodrugs may be found in Prodrugs as Novel Delivery Systems, Vol. 14, ACS Symposium Series (T. Higuchi and V.J. Stella) and Bioreversible Carriers in Drug Design, Pergamon Press, 1987 (ed. E. B. Roche, American Pharmaceutical Association; C.S. Larsen and J. 0stergaard, Design and application of prodrugs, In Textbook of Drug Design and Discovery, 3rd Edition, 2002, Taylor and Francis).
Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of formula (I) with certain moieties known to those skilled in the art as 'pro -moieties' as described, for example, in Design of Prodrugs by H. Bundgaard (Elsevier, 1985). Such examples could be a prodrug of a carboxyl group (such as -CO-0-CH2-0-CO-tBu as used in the pivampicillin prodrug of ampicillin), an amide (-CO-NH-CH2-NAlk2) or an amidine (-C(=N-0-CH3)-NH2).
Embodiments of the Invention
It is to be understood that all preferred groups or embodiments described herebelow for compounds of formula (I) may be combined among each other and apply as well to compounds of formula (la), (lb), (Ic), (IA), (IB), (IC), (ID) or (IE) as below defined mutatis mutandis.
It is also to be understood that the proviso above described for compounds of formula (I) should be applied mutatis mutandis to all the preferred groups or embodiments described herebelow.
In one embodiment, compounds of formula (la) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which is linked to group W and identified with number (1) herebelow, possess the absolute configuration herebelow represented:
Figure imgf000023_0001
In another embodiment, compounds of formula (lb) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which are linked to group W and Y and identified, respectively, with numbers (1) and (2) herebelow, possess the absolute configuration herebelow represented
Figure imgf000023_0002
In a further embodiment, compound of formula (Ic) are provided, which are compounds of formula (I) as above defined wherein carbon stereogenic center on the cycloalkylene portion of ring A which are linked to group W and Y and identified, respectively, with numbers (1) and (2) herebelow, possess the absolute configuration herebelow represented:
Figure imgf000023_0003
In one embodiment W is NH or O. In a further embodiment, W is NH.
In one embodiment Y is a group -S(0)p-, a group -0(CR3R4)n-, a group -(CR5R6)„-, or a group -NR7-; p is zero and n is 0, 1 or 2. In another embodiment, Y is a group -S(0)p- or a group -0(CR3R4)n or; p is zero and n is 0 or 1. In a further embodiment Y is a group -0(CR3R4)n- and n is 0.
In one embodiment R3, R4, R5 and R6 are each independently -H, fluorine or Ci-C6 alkyl. In another embodiment, R3, R4, R5 and R6 are -H.
In one embodiment R7 is -H, Ci-C6 alkyl, or C3-C7 cycloalkyl.
In one embodiment R7 is -H.
In one embodiment A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms; said cycloalkylene ring being attached to W and Y, and optionally fused to a phenyl ring or to a monocyclic heteroaryl ring having 5 or 6 ring atoms, such phenyl or heteroaryl ring being optionally substituted by one or two groups R24.
In a further embodiment, A is group selected in the group consisting of:
Figure imgf000024_0001
In a further embodiment, A is
*
Figure imgf000024_0002
still further embodiment, A is group
Figure imgf000024_0003
In an additional embodiment, A is group
Figure imgf000024_0004
In one embodiment R24 is not present or, if present, is independently selected from the group consisting of C1-C2 alkyl,-F, -CI and -CN; in a further embodiment, R24 is not present or, if present, is at each occurrence independently methyl or -F. In a further embodiment, R24 is not present.
In a further embodiment, R1 i a (Ila)
Figure imgf000025_0001
(Ila)
In one embodiment, the group (Ila) is a group of formula (Ilaa) or (Ilab) which is connected to the group Y through one of the carbons as below indicated
Figure imgf000025_0002
(Ilaa) (Ilab)
In another embodiment, the group (Ila) is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
Figure imgf000025_0003
In one embodiment, X4 is a carbon atom.
In one embodiment, X5 is a nitrogen atom.
In another embodiment, X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is nitrogen.
In another embodiment, X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is group -CH-.
In another embodiment, X4 is a nitrogen atom, X5 is a group -CH- atom, X3 is a carbon atom and X2 is group -CH-.
In one embodiment, R13 is -H, Ci_C6 alkyl or halo.
In a further embodiment, the group (Ila) is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
R 11
Figure imgf000026_0001
and wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H.
In one embodiment R11 is selected from a group consisting of -NRARB, -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-NRARB, -(Ci- C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C2-C6alkylene)-NRARB, -O- (C3-C7cycloalkylene)-NRARB, ORc and -(C3-C7heterocycloalkyl), wherein any of such -(Ci-Cealkylene)- -(C2-C6alkylene)-, -(C3-C7cycloalkylene)- portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the list consisting of Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo.
In one embodiment R11 is a group
Figure imgf000026_0002
wherein R25 is optionally present and represents one, two or three substituents independently selected in the list consisting of Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In a further embodiment, R11 is a group
Figure imgf000027_0001
wherein R represents one, two or three substituents independently selected in the list consisting of Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In a further embodiment, R11 is a group
Figure imgf000027_0002
wherein R represents one or two Ci-C6 alkyl substituents; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In a further embodiment, R11 is a group
Figure imgf000027_0003
wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In a further embodiment, R11 is a group
Figure imgf000027_0004
wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In a still further embodiment, R11 is a group
Figure imgf000028_0001
wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4.
In another embodiment R11 is phenyl which is optionally substituted by one or more group(s) selected from halo, Ci-C6 alkyl which is substituted by one or more group(s) -CN; -(Ci-Cealkylene)-NRARB, wherein RA and RB may form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups selected from -ORD, -CN, -NRCRD, halo, Ci-C6 alkyl or C3-C7 cycloalkyl; and which 6-1 1- membered saturated heterocyclic monocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl.
In one embodiment R25 is one, two or three groups independently selected in the list consisting of Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo.
In another embodiment RA and RB form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more groups independently selected from -ORD, -CN, -NRA RB', halo, Ci-C6 alkyl, -(C3- Cy) cycloalkyl-NRA'RB', -(Ci-C5 alkylene)-NRA RB', -0-(Ci-C5 alkylene)-NRA RB' and -0-(C3-C7cycloalkyl)-NRA'RB', -N(Rc')C(0)-(Ci-C6alkylene)-NRA'RB', -N(Rc')C(0)-(C3-C7cycloalkylene)-NRA'RB', -C(0)N(Rc')-(C2-C6alkylene)-NRA'RB', -C(0)N(Rc')-(C3-C7cycloalkylene)-NRA'RB' such Ci-C6 alkyl, C2-C5 alkyl and C3-C7 cycloalkyl portions being optionally substituted by a group C1-C3 alkyl, C3- C7cycloalkyl, -ORD, -CN, -NRA RB or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD, -CN, or halo; or RA and RB may be linked to one carbon atom of the -(Ci-Cealkylene)- or -(C3-Cycycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms.
In another embodiment RA and RB form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1- membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups independently selected from Ci-C6 alkyl, -(C1-C5 alkylene)-NRA RB' and -0-(Ci-C5 alkylene)-NRA RB such Ci-C6 alkyl being optionally substituted by a group C1 -C3 alkyl or halo.
In another embodiment RA and RB form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C1-C5 alkylene)-NRA RB , wherein RA and RB are independently -H or Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl.
In one embodiment R17 is a group of general formula (IV)
Figure imgf000029_0001
wherein R19 is selected in a group consisting of -H, -CH3, -CN and OH; R20 is selected in the group consisting of: -H, -F, -CH3, -C2H5 and -CH2OH; R21 is -H,
Figure imgf000029_0002
In one embodiment R 1i9y is -H, R 2z0u and R 21 are -CH3.
19 20 21 *
In one embodiment R and R are H and RZ1 is -CH3.
In one embodiment R 19 , R 2z0u and R 21 are -H.
In one embodiment R 19 , R 20 and R 21 are -CH3.
In one embodiment R18 is absent or is selected in the group consisting of aryl, heteroaryl and -(Ci-C6alkyl) wherein any of such aryl, heteroaryl and -(Ci-C6alkyl) may be optionally substituted by one or more group(s) selected from -OH, -Ci- C6alkyl, C3-C6cycloalkyl, -0-(Ci-C6alkyl), -0-(C3-C6cycloalkyl), -NRHRJ, -N(RL)(C2-C6alkylene)-NRHRJ, -N(RL)(C3-C7cycloalkylene)-NRHRJ, -(Ci- C6alkylene)-NRHRJ, -(C3-C7cycloalkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ, -O- (C3-C7cycloalkylene)-NRHRJ, ORL, wherein any of such Ci-C6alkyl, C3- C6cycloalkyl, -(Ci-Cealkylene)-, -(C2-Cealkylene)- and -(C3-C7cycloalkylene)- portion in the above listed groups may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo.
In one embodiment R18 is aryl optionally substituted by one or more group(s) selected from -Ci-C6alkyl, -(Ci-C6alkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ wherein any of such Ci-C6alkyl, -(Ci-Cealkylene)- and -(C2-Cealkylene)- portion in the above listed groups may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo.
In one embodiment RH and RJ are at each occurrence independently Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, -CN, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORM, -CN or halo; and which 6- 1 1 - membered saturated monocyclic or bicyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3- C7 cycloalkyl, -ORM, -CN, or halo.
In another embodiment RH and RJ may form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, NR°RP or Ci-C6 alkyl wherein such Ci-C6 alkyl is optionally substituted by a group C1-C3 alkyl, -ORM or halo; and which 6-1 1- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORM, -CN or halo.
In one embodiment RL is independently -H, Ci -C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group C1-C3 alkyl, -ORM, -CN or halo.
In one embodiment RM is independently -H, Ci -C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group hydroxyl, -CN or halo.
In one embodiment R° and RP are each independently Ci-C6 alkyl or Ci-C6 cycloalkyl, optionally substituted by a group C1-C3 alkyl or -ORQ; alternatively, R° and RP may form together with the nitrogen atom to which they are attached a 4-8- membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORQ, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, and which 6-8- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl.
In another embodiment R° and RP are each independently Ci-C6 alkyl or Ci- C6 cycloalkyl, optionally substituted by a group C1-C3 alkyl.
In one embodiment RQ is -H, -CH3 or -C2H5.
In one embodiment z1=-CH-, z2=C, z3 is O or N and z4 is N.
In an additional embodiment R2 is a radical of formula (Ilia)
Figure imgf000032_0001
wherein z 1 =-CH-, z 2 = C, z3 is O, z 4 is N, R 1 8 is absent, and R 17 is a group of general formula (IV)
Figure imgf000032_0002
wherein R19, R20 and R21 are -CH3.
further embodiment, R is a radical of formula (Ilia)
Figure imgf000032_0003
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000032_0004
wherein R19 is -H, R20 and R21 are -CH3.
In another embodiment, R2 is a radical of formula (Ilia)
R17\ N 2 1
z
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
(IV)
Figure imgf000033_0001
Riy and R2U are H and R is -CH3.
In a further embodiment R2 is a radical of formula (Ilia)
Figure imgf000033_0002
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000033_0003
wherein R19, R20 and R21 are -H.
In an additional embodiment R2 is a radical of formula (Ilia)
Figure imgf000033_0004
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000033_0005
19 20 21 18
wherein R , Rzu and RZ1 are -CH3 and R is absent or is selected from -(Ci- C6alkyl), -(Ci-C6alkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ or aryl, wherein any portion in the above listed groups may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo.
In another embodiment R is a radical of formula (Ilia)
Figure imgf000034_0001
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000034_0002
18
wherein Ri y is -H, Rzu and RZ 1 are -CH3 and R is heteroaryl substituted by a
-(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached a 4- 1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl; and which 6- 1 1 - membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -CN, or halo.
In another embodiment R2 is a radical of formula (Ilia)
Figure imgf000034_0003
(Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000034_0004
18
wherein Ri y and are -H, and R is -CH3 and R is heteroaryl substituted by a -(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1-C3 alkyl, -CN or halo.
In another embodiment R2 is a radical of formula (Ilia)
\ 2 1
z
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000035_0001
19 20 21 18
wherein R , Rzu and RZ 1 are -CH3 and R is aryl or heteroaryl optionally substituted by a group selected from -(Ci-C6alkyl), -0-(Ci-C6alkylene)-NRHRJ or - (Ci-C6alkylene)-NRHRJ and wherein A is a group
In another embodiment R is a radical of formula (Ilia)
Figure imgf000035_0003
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000035_0004
19 20 21 18
wherein R , Rzu and RZ 1 are -CH3 and R is aryl optionally substituted by -(Ci-Cealkyl), -(C2-C6alkylene)-NRHRJ or -0-(C2-C6alkylene)-NRHRJ.
In another embodiment R is a radical of formula (Ilia)
Figure imgf000036_0001
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000036_0002
wherein 19 20 21 18
R , Rzu and RZ 1 are H and R is phenyl optionally substituted by a -0-(C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, -CN or halo.
In another embodiment R2 is a radical of formula (Ilia)
Figure imgf000036_0003
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000036_0004
19 20 21 18
wherein R , Rzu and RZ 1 are H and R is phenyl optionally substituted by -O- (C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl.
In one embodiment, compounds of formula (I A) are provided wherein W is NH, Y is a group -0(CR3R4 - and n is 0, A is group
Figure imgf000036_0005
or
wherein R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
Figure imgf000037_0001
R (Ilaa)
and wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H;
wherein R11 is a group:
Figure imgf000037_0002
wherein R represents one, two or three substituents independently selected in the list consisting of: Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4;
wherein R2 is a radical of formula (Ilia)
R17\ N 2 1
(Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000037_0003
19 20 21 18
wherein R , Rzu and RZ 1 are -CH3 and wherein R is phenyl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -O- (C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1-C3 alkyl, -CN or halo.
In one embodiment, compounds of formula (IB) are provided wherein W is NH, Y is a group -0(CR3R4 - and n is 0, A is group
Figure imgf000038_0001
or
wherein R1 is a group of formula Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
Figure imgf000038_0002
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group -NRARB, wherein RA and RB form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C1 -C5 alkylene)-NRA RB', wherein RA' and RB' are independently -H or Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, C3-Cycycloalkyl, -ORD , -CN or halo; or R is a group
Figure imgf000038_0003
wherein R represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (C1 -C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4; wherein R2 is a radical of formula (Ilia)
(Ilia) wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000039_0001
wherein R 19 , R2z0u and R 2Z11 are -CH3 and wherein R 18 is heteroaryl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -O- (C2-C6alkylene)-NRHRJ, wherein RH and RJ form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, -CN, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3- C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, wherein R° and RP are each independently Ci-C6 alkyl.
In one embodiment, compounds of formula (IC) are provided wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group:
Figure imgf000039_0002
R is a group:
Figure imgf000039_0003
wherein R25 is optionally present and represents one or two substituents independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4; R2 is a radical of formula (Ilia)
Figure imgf000040_0001
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000040_0002
wherein R19, R20 and R21 are H and R18 is phenyl optionally substituted by -O- (Ci-C6alkylene)-NRHRJ, wherein RH and RJ are Ci-C6 alkyl.
In one embodiment, compounds of formula (ID) are provided wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000040_0003
R1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjace
Figure imgf000040_0004
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group:
Figure imgf000040_0005
wherein R25 is optionally present and represents one two or three substituents independently selected in the list consisting of: Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4; wherein R2 is a radical of formula (Ilia):
Figure imgf000041_0001
(Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000041_0002
wherein R 1i9y is -H, R 2z0u and R 2Z11 are -CH3 and R 18 is heteroaryl substituted by a -(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl; and which 6-1 1- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -CN or halo.
In one embodiment, compounds of formula (IE) are provided wherein W is
NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000041_0003
R1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
Figure imgf000042_0001
R " (Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is a group:
Figure imgf000042_0002
wherein R is optionally present and represents one two or three substituents independently selected in the list consisting of: Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4; wherein R2 is a radical of formula (I
Figure imgf000042_0003
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000042_0004
19 20 21 18
wherein R and R are H and R is -CH3 and R is heteroaryl substituted by a -(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, -CN or halo.
In one embodiment, compounds of formula (IF) are provided wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000043_0001
wherein R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
Figure imgf000043_0002
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is a group -NRARB, wherein RA and RB form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(C1 -C5 alkylene)-NRA RB', wherein RA' and RB' are independently -H or Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, C3-C7cycloalkyl, -ORD', -CN or halo; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1 z
' 18
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000043_0003
R 19 , R2z0u 21 CH3 18
wherein and RZ1 are - and wherein R is -Ci-C6alkyl, optionally substituted by Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo.
In one embodiment, compounds of formula (IG) are provided wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000044_0001
wherein R is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X2
D 11
Figure imgf000044_0002
R (Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is phenyl which is optionally substituted by one or more group(s) selected from halo, Ci-C6 alkyl, wherein such Ci-C6 alkyl is optionally substituted by -(Ci-Cealkylene)-NRARB, wherein RA and RB may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups selected from -ORD, -CN, -NRCRD, halo, Ci-C6 alkyl or C3-C7 cycloalkyl; and which 6- 1 1 - membered saturated heterocyclic monocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl; wherein R2 is a radical of formula (Ilia)
Figure imgf000044_0003
(Ilia)
1 2 3 4 18 17
wherein z =-CH-, z = C, z is O, z is N, R is absent, and R is a group general formula (IV)
Figure imgf000044_0004
(IV) wherein R19, R20 and R21 are -CH3 and R18 is -(Ci-C6alkyl).
In one embodiment, compounds of formula (IH) are provided wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group:
Figure imgf000045_0001
wherein R1 is a group of formula (Ilaa) as above defined which is connected to the group Y through the carbon adjacent to X
11
Figure imgf000045_0002
wherein X is a carbon atom, X is a nitrogen atom, X is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group
Figure imgf000045_0003
wherein R represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1
Z
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
(IV)
Figure imgf000046_0001
wherein R 19 , R2z0u and R 2Z11 are -CH3 and wherein 18
R is aryl or heteroaryl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -0-(C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo.
In one embodiment, compounds of formula (IL) are provided wherein W is NH, Y is a group -0 CR3R4)n- and n is 0, A is group
Figure imgf000046_0002
or
R is a group:
Figure imgf000046_0003
wherein R is optionally present and represents one or two substituents independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; and wherein the asterisk represents the point of attachment for group R1 1 to the rest of the molecule via X4;
R2 is a radical of formula (Ilia)
Figure imgf000046_0004
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
(IV)
Figure imgf000047_0001
19 20 21
wherein R , Rzu and RZ1 are CH3 and R 18 is phenyl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -0-(C2-Cealkylene)- NRHRJ, wherein RH and RJ are Ci-C6 alkyl or RH and RJ form together with the nitrogen atom to which they are attached an azetidine or a 4-11 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups halo or NR°RP, wherein R° and RP are each independently Ci-C6 alkyl.
In one embodiment, a compound of formula (I) is selected in the list consisting of:
· l-[ -(2-Dimethylamino-ethyl)-3-isopropyl- H-[l,4']bipyrazolyl-5-yl]-3-
{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-{3-isopropyl- -[2-(4- methyl-piperazin-l-yl)-ethyl]- H-[l,4']bipyrazolyl-5-yl}-urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[3-isopropyl-l'-(2- pyrrolidin-l-yl-ethyl)- H-[l,4']bipyrazolyl-5-yl]-urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[3-isopropyl-l'-(2-
[l,4]oxazepan-4-yl-ethyl)-l'H-[l,4']bipyrazolyl-5-yl]-urea formate salt;
• 1 - [ 1 '-(2-Dimethylamino-ethyl)-3 -isopropyl- 1 Ή- [ 1 ,3 ']bipyrazolyl-5 -yl] -3 -
{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
· l-[ -(2-Dimethylamino-ethyl)-3-isopropyl- H-[l,4']bipyrazolyl-5-yl]-3-
{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1,2,3,4-tetrahydro-naphthalen-l-yl}- urea formate salt;
l-Cr-C -Dimethylamino-ethy -S-ethyl-rH-Cl^^bi yrazolyl-S-y^-S-lilS^R)- 4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4- tetrahydro-naphthalen-l-yl}- urea formate salt;
· l-[l*-(2-Dimethylamino-ethyl)-3-ethyl-l*H-[l,4,]bipyrazolyl-5-yl]-3-{(lS,4R)- 4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
• l-{2-[3-(2-Dimethylamino-ethoxy)-phenyl]-5-methyl-2H-pyrazol-3-yl}-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
1 - [ 1 *-(2- Azetidin- 1 -yl-ethyl)-3 -tert-butyl- 1 Ή- [ 1 ,4*]bipyrazolyl-5 -yl] -3 - {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
1 - {3-tert-Butyl- 1 '-[2-(3-dimethylamino-azetidin- 1 -yl)-ethyl]- 1 Ή- [l,4']bipyrazolyl-5-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-
[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
• l-{5-tert-Butyl-2-[3-(3-dimethylamino-azetidin-l-ylmethyl)-phenyl]-2H- pyrazol-3-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((lS,4R)-4-{3-[2,6-dichloro-4-(4- methyl-piperazin-l-ylmethyl)-phenyl]-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy}- l,2,3,4-tetrahydro-naphthalen-l-yl)-urea formate salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4- tetrahydro-naphthalen-l-yl}-urea formate salt;
l-(5-tert-Butyl-isoxazol-3-yl)-3-{(lS,4R)-4-[3-(4-dimethylaminomethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen- l-yl}-urea;
1 -(3 -tert-Butyl- 1 '-methyl- 1 Ή
dimethylaminomethyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l ^ tetrahydro-naphthalen-l-yl}-urea formate salt;
· l-[3-tert-Butyl-l *-(2-dimethylamino-ethyl)-l*H-[l ,4,]bipyrazolyl-5-yl]-3- {4-[3- ((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl} -urea;
l-(5-tert-Butyl)-2-p-tolyl-2H-pyrazol-3-yl)-3- {4-[3-((S)-2-methyl-piperidin-l- yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-cyclohexyl}-urea;
· l- {5-tert-Butyl-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H-pyrazol-3-yl}-3- {4-[3-((S)-2-methyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl} -urea;
• l- {5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3- {(l S,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3- a]pyridin-6-yloxy]- 1 ,2,3 ,4-tetrahydronaphthalen- 1 -yl} -urea;
• l- {5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3- {(l S,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)- [l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l ,2,3,4-tetrahydro-naphthalen-l-yl} -urea;
1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(2-dimethylamino- ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrahydro- naphthalen-l-yl)-urea hydrogen chloride salt; l-[5-tert-Butyl-2-(2-hydroxy-ethyl)-2H-pyrazol-3-yl]-3-((lS,4R)-4-{3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt; · l-(3-tert-Butyl-l'-methyl-l,H-[l,4,]bipyrazolyl-5-yl)-3-((lS,4R)-4-{3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt; l-(5-tert-Butyl-isoxazol-3-yl)-3-((lS,4R)-4-{3-[4-(2-dimethylamino-ethoxy)- piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrahydro-naphthalen- 1 - yl)-urea hydrogen chloride salt;
1 -(5-tert-Butyl-2-propyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(2-dimethylamino- ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrahydro- naphthalen- l-yl)-urea hydrogen chloride salt; l-[5-tert-Butyl-2-(2-methoxy-ethyl)-2H-pyrazol-3-yl]-3-((lS,4R)-4-{3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen- l-yl)-urea hydrogen chloride salt; · l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-((S)-3- {[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1, 2,3, 4-tetrahydro-naphthalen-l-yl} -urea hydrogen chloride salt; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((S)-3- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen- 1 -yl} -urea hydrogen chloride salt; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(4- {[(2-methoxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3 ,4-tetrahydro-naphthalen- 1 -yl} -urea; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(4- {[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1,2,3, 4-tetrahydro-naphthalen-l-yl} -urea hydrogen chloride salt; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4-[l,4]oxazepan-4- ylmethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-urea hydrogen chloride salt; · 1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(4-methyl- piperazin- 1 -ylmethyl)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((R)-3-{[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1, 2,3, 4-tetrahydro-naphthalen-l-yl} -urea hydrogen chloride salt; · 1 -[5-tert-Butyl-2-(2-methoxy-ethyl)-2H-pyrazol-3-yl]-3- {(1 S,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen- 1 -yl} -urea; l-(5-tert-Butyl-2-ethyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen- 1 -yl} -urea formate salt;
N- [5 -tert-Butyl-3 -(3 -{(IS ,4R)-4- [3 -(4-dimethylaminomethyl-piperidin- 1 -yl)- [ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4-tetrahydro-naphthalen- 1 -yl} -ureido)-2- methoxyphenyljmethanesulfonamide hydrogen chloride salt; l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((2S,4S)-4- dimethylamino-l-methyl-pyrrolidin-2-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4- tetrahydro-naphthalen-l-yl}-urea hydrochloride salt;
1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(l ,4-dimethyl- 1 ,4,9- triaza-spiro[5.5]undec-9-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-urea hydrochloride salt and pharmaceutically acceptable salts thereof.
Utility
As mentioned above the compounds of the invention are p38MAPK inhibitors, and thus may have utility for the treatment of diseases or conditions which benefit from inhibition of the p38 enzyme. Such diseases and conditions are known from the literature and several have been mentioned above. However, the compounds are generally of use as anti-inflammatory agents, particularly for use in the treatment of respiratory disease. In particular, the compounds may be used in the treatment of chronic obstructive pulmonary disease (COPD), chronic bronchitis, lung fibrosis, pneumonia, acute respiratory distress syndrome (ARDS), pulmonary emphysema, or smoking-induced emphysema, intrinsic (non-allergic asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, steroid resistant asthma, neutrophilic asthma, bronchitic asthma, exercise induced asthma, occupational asthma and asthma induced following bacterial infection, cystic fibrosis, pulmonary fibrosis and bronchiectasis.
The present invention provides the use of the compounds of the invention for the prevention and/or treatment of any disease or condition which benefit from inhibition of the p38 enzyme.
In a further aspect the present invention provides the use of compounds of the invention for the preparation of a medicament for the prevention and/or treatment of any disease or condition which benefit from inhibition of the p38 enzyme.
Moreover the present invention provides a method for prevention and/or treatment of any disease which benefit from inhibition of the p38 enzyme, said method comprises administering to a patient in need of such treatment a therapeutically effective amount of a compound of the invention.
Compositions
As mentioned above, the compounds with which the invention is concerned are p38 kinase inhibitors, and are useful in the treatment of several diseases for example inflammatory diseases of the respiratory tract. Examples of such diseases are referred to above, and include asthma, rhinitis, allergic airway syndrome, bronchitis and chronic obstructive pulmonary disease.
It will be understood that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing treatment. Optimum dose levels and frequency of dosing will be determined by clinical trial, as is required in the pharmaceutical art. In general, the daily dose range for oral administration will lie within the range of from about 0.001 mg to about 100 mg per kg body weight of a human, often 0.01 mg to about 50 mg per kg, for example 0.1 to 10 mg per kg, in single or divided doses. In general, the daily dose range for inhaled administration will lie within the range of from about 0.1 μg to about lmg per kg body weight of a human, preferably 0.1 μg to 50 μg per kg, in single or divided doses. On the other hand, it may be necessary to use dosages outside these limits in some cases. For the purpose of the invention, inhaled administration is preferred.
The compounds with which the invention is concerned may be prepared for administration by any route consistent with their pharmacokinetic properties. Orally administrable compositions may be in the form of tablets, capsules, powders, granules, lozenges, liquid or gel preparations, such as oral, topical, or sterile parenteral solutions or suspensions. Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricant, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants for example potato starch, or acceptable wetting agents such as sodium lauryl sulfate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, glucose syrup, gelatin hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, fractionated coconut oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl /?-hydroxybenzoate or sorbic acid, and if desired conventional flavouring or colouring agents.
For topical application to the skin, the drug may be made up into a cream, lotion or ointment. Cream or ointment formulations which may be used for the drug are conventional formulations well known in the art, for example as described in standard textbooks of pharmaceutics such as the British Pharmacopoeia.
The active ingredient may also be administered parenterally in a sterile medium. Depending on the vehicle and concentration used, the drug can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle.
However, for treatment of an inflammatory disease of the respiratory tract, compounds of the invention may also be formulated for inhalation, for example as a nasal spray, or dry powder or aerosol inhalers. For delivery by inhalation, the active compound is preferably in the form of microparticles. They may be prepared by a variety of techniques, including spray-drying, freeze-drying and micronisation. Aerosol generation can be carried out using, for example, pressure-driven jet atomizers or ultrasonic atomizers, preferably using propellant-driven metered aerosols or propellant-free administration of micronized active compounds from, for example, inhalation capsules or other "dry powder" delivery systems.
By way of example, a composition of the invention may be prepared as a suspension for delivery from a nebuliser or as an aerosol in a liquid propellant, for example for use in a pressurised metered dose inhaler (PMDI). Propellants suitable for use in a PMDI are known to the skilled person, and include CFC-12, HFA-134a, HFA-227, HCFC-22 (CC12F2) and HFA-152 (CH4F2 and isobutane).
In a preferred embodiment of the invention, a composition of the invention is in dry powder form, for delivery using a dry powder inhaler (DPI). Many types of DPI are known.
Microparticles for delivery by administration may be formulated with excipients that aid delivery and release. For example, in a dry powder formulation, microparticles may be formulated with large carrier particles that aid flow from the DPI into the lung. Suitable carrier particles are known, and include lactose particles; they may have a mass median aerodynamic diameter of greater than 90 μιη.
In the case of an aerosol-based formulation, an example is:
Compound of the invention 24 mg / canister
Lecithin, NF Liq. Cone. 1.2 mg / canister
Trichlorofluoromethane, NF4.025 g / canister
Dichlorodifluoromethane, NF 12.15 g / canister.
The active compounds may be dosed as described depending on the inhaler system used. In addition to the active compounds, the administration forms may additionally contain excipients, such as, for example, propellants (e.g. Frigen in the case of metered aerosols), surface-active substances, emulsifiers, stabilizers, preservatives, flavorings, fillers (e.g. lactose in the case of powder inhalers) or, if appropriate, further active compounds.
For the purposes of inhalation, a large number of systems are available with which aerosols of optimum particle size can be generated and administered, using an inhalation technique which is appropriate for the patient. In addition to the use of adaptors (spacers, expanders) and pear-shaped containers (e.g. Nebulator®, Volumatic®), and automatic devices emitting a puffer spray (Autohaler®), for metered aerosols, in particular in the case of powder inhalers, a number of technical solutions are available (e.g. Diskhaler®, Rotadisk®, Turbohaler® or the inhalers for example as described EP-A-0505321). Additionally, compounds of the invention may be delivered in multi-chamber devices thus allowing for delivery of combination agents. Combinations
Other compounds may be combined with compounds with which the invention is concerned for the prevention and treatment of inflammatory diseases, in particular respiratory diseases. Thus the present invention is also concerned with pharmaceutical compositions comprising a therapeutically effective amount of a compound of the invention and one or more other therapeutic agents. Suitable therapeutic agents for a combination therapy with compounds of the invention include, but are not limited to: (1) corticosteroids, such as fluticasone propionate, fluticasone furoate, mometasone furoate, beclometasone dipropionate, ciclesonide, budesonide, GSK 685698, GSK 870086, QAE 397, QMF 149, TPI-1020; (2) β2- adrenoreceptor agonists such as salbutamol, albuterol, terbutaline, fenoterol, and long acting 2-adrenoreceptor agonists such as salmeterol, indacaterol, formoterol (including formoterol fumarate), arformoterol, carmoterol, GSK 642444, GSK 159797, GSK 159802, GSK 597501 , GSK 678007, AZD3199, vilanterol, olodaterol and abediterol; (3) corticosteroid/long acting β2 agonist combination products such as salmeterol/ fluticasone propionate (Advair/Seretide), formoterol/budesonide (Symbicort), formoterol/fluticasone propionate (Flutiform), formoterol/ciclesonide, formoterol/mometasone furoate, formoterol/ beclometasone dipropionate, indacaterol/mometasone furoate, Indacaterol/QAE 397, GSK 159797/GSK 685698, GSK 159802/GSK 685698, GSK 642444/GSK 685698, GSK 159797/GSK 870086, GSK 159802/GSK 870086, GSK 642444/GSK 870086, arformoterol/ciclesonide;(4) anticholinergic agents, for example muscarinic-3 (M3) receptor antagonists such as ipratropium bromide, tiotropium bromide, Aclidinium (LAS-34273), NVA-237, GSK 233705, Darotropium, GSK 573719, GSK 961081 , QAT 370, QAX 028, EP- 101 ; (5) dual pharmacology M3-anticholinergic/ 2-adrenoreceptor agonists such as GSK961081 , AZD21 15 and LAS 190792; (6) leukotriene modulators, for example leukotriene antagonists such as montelukast, zafirulast or pranlukast or leukotriene biosynthesis inhibitors such as Zileuton or BAY- 1005, or LTB4 antagonists such as Amelubant, or FLAP inhibitors such as GSK 2190914, AM- 103; (7) phosphodiesterase-IV (PDE-IV) inhibitors (oral or inhaled), such as rof umilast, cilomilast, Oglemilast, ONO-6126, Tetomilast, Tofimilast, UK 500,001 , GSK 256066; (8) antihistamines, for example selective histamine-1 (HI) receptor antagonists, such as fexofenadine, citirizine, loratidine or astemizole or dual H1/H3 receptor antagonists such as GSK 835726, GSK 1004723, or selective histamine-4 (H4) receptor antagonists, such as ZPL3893787; (9) antitussive agents, such as codeine or dextramorphan; (10) a mucolytic, for example N acetyl cysteine or fudostein; (1 1) a expectorant/mucokinetic modulator, for example ambroxol, hypertonic solutions (e.g. saline or mannitol) or surfactant; (12) a peptide mucolytic, for example recombinant human deoxyribonoclease I (dornase-alfa and rhDNase) or helicidin; (13) antibiotics, for example azithromycin, tobramycin and aztreonam; (14) non-selective COX-1 / COX-2 inhibitors, such as ibuprofen or ketoprofen; (15) COX-2 inhibitors, such as celecoxib and rofecoxib; (16) VLA-4 antagonists, such as those described in WO97/03094 and WO97/02289; (17) TACE inhibitors and TNF-a inhibitors, for example anti-TNF monoclonal antibodies, such as Remicade and CDP-870 and TNF receptor immunoglobulin molecules, such as Enbrel; (18) inhibitors of matrix metalloprotease, for example MMP-12; (19) human neutrophil elastase inhibitors, such as ONO-6818 or those described in WO2005/026124, WO2003/053930 and WO06/082412; (20) A2b antagonists such as those described in WO2002/42298; (21) modulators of chemokine receptor function, for example antagonists of CCR3 and CCR8; (22) compounds which modulate the action of other prostanoid receptors, for example a thromboxane A2 antagonist; DP I antagonists such as MK-0524, CRTH2 antagonists such as ODC9101 and OC000459 and AZD 1981 and mixed DP1/CRTH2 antagonists such as AMG 009 and AMG853; (23) PPAR agonists including PPAR alpha agonists (such as fenofibrate), PPAR delta agonists, PPAR gamma agonists such as Pioglitazone, Rosiglitazone and Balaglitazone; (24) methylxanthines such as theophylline or aminophylline and methylxanthine/corticosteroid combinations such as theophylline/budesonide, theophylline/fluticasone propionate, theophylline/ciclesonide, theophylline/mometasone furoate and theophylline/beclometasone dipropionate; (25) A2a agonists such as those described in EP 1052264 and EP1241 176; (26) CXCR2 or IL-8 antagonists such as SCH 527123 or GSK 656933; (27) IL-R signalling modulators such as kineret and ACZ 885; (28) MCP-1 antagonists such as ABN-912.
The invention is also directed to a kit comprising the pharmaceutical compositions of compounds of the invention alone or in combination with or in admixture with one or more pharmaceutically acceptable carriers and/or excipients and a device which may be a single- or multi-dose dry powder inhaler, a metered dose inhaler or a nebulizer.
Methods of synthesis
In one aspect of the present invention, a process for the preparation of compounds of the invention is provided, according to general synthetic routes described in this section. In the following reaction schemes, unless otherwise indicated, the groups mentioned assume the same meaning as those reported for compounds of formula (I).
The skilled person may introduce, where appropriate, suitable variations to the conditions specifically described in the experimentals in order to adapt the synthetic routes to the provision of further compounds of the invention. Such variations may include, but are not limited to, use of appropriate starting materials to generate different compounds, changes in the solvent and temperature of reactions, replacements of reactives with analogous chemical role, introduction or removal of protection/deprotection stages of functional groups sensitive to reaction conditions and reagents, as well as introduction or removal of specific synthetic steps oriented to further functionalisation of the chemical scaffold.
Processes which can be used and are described and reported in Examples and Schemes, should not be viewed as limiting the scope of the synthetic methods available for the preparation of the compounds of the invention.
The process described is particularly advantageous as it is susceptible of being properly modulated, through any proper variant known to the skilled person, so as to obtained any of the desired compounds of the invention. Such variants are comprised within the scope of the present invention.
From all of the above, it should be clear to the skilled person that any of the described groups may be present as such or in any properly protected form.
In particular, functional groups present in the intermediate and compounds and which could generate unwanted side reaction and by-products, need to be properly protected before the alkylation, acylation, coupling or sulfonylation takes place. Likewise, subsequent deprotection of those same protected groups may follow upon completion of the said reactions.
In the present invention, unless otherwise indicated, the term "protecting group" designates a protective group adapted to preserve the function of the group it is bound to. Typically, protective groups are used to preserve amino, hydroxyl, or carboxyl functions. Appropriate protecting groups may thus include, for example, benzyl, benzyloxycarbonyl, t-butoxycarbonyl, alkyl or benzyl esters or the like, which are well known to those skilled in the art [see, for a general reference, T.W. Green; Protective Groups in Organic Synthesis (Wiley, N.Y. 1981)].
Likewise, selective protection and deprotection of any of the said groups, for instance including carbonyl, hydroxyl or amino groups, may be accomplished according to very well known methods commonly employed in organic synthetic chemistry.
Optional salification of the compounds of formula (I) or N-oxides on the pyridine ring thereof may be carried out by properly converting any of the free acidic or amino groups into the corresponding pharmaceutically acceptable salts. In this case too, the operative conditions being employed for the optional salification of the compounds of the invention are all within the ordinary knowledge of the skilled person.
From all of the above, it should be clear that the above process, comprehensive of any variant thereof for the preparation of suitable compounds of the invention, may be conveniently modified so that to adapt the reaction conditions to the specific needs, for instance by choosing appropriate condensing agents, solvents and protective groups, as the case may be.
For example compounds of the invention of formula (I) may be prepared according to the route illustrated in Scheme 1.
Figure imgf000060_0001
Scheme 1
Compounds of general formula (I) may be prepared from compounds of general formula (la) by reaction with a compound of general formula (lbl) or (lb2) wherein R2 is as defined in general formula (I), in a suitable solvent such as dimethyl sulfoxide, 1 ,4-dioxane, Ν,Ν-dimethylformamide or acetonitrile, in the presence of a base such as diisopropylethylamme at a range of temperatures, preferably between room temperature and 100°C.
Compounds of general formula (lb l) and (lb2) are either known in the literature or may be prepared from amines of general formula (lc) according to known literature procedures (e.g. see for reference WO2006009741 , EP 1609789).
Compounds of general formula (lc) are either known in the literature or may be synthesised by one skilled in the art by adapting appropriate literature methods (e.g WO2010077836, WO2006009741 , WO2008125014, J. Med Chem., 2007, 50, 4016, Bulletin des Societes Chimiques Beiges, 1987, 96, 675-709, Organic & Biomolecular Chemistry, 2006, 4, 4158-4164).
Compounds of general formula (lea), i.e. compounds of formula (lc) wherein
2 17 18 1 2 3 4
R is a group of formula (Illb) and R , R , z , z , z and z are as defined above can be prepared from compounds of formula (l e),
Figure imgf000061_0001
using a suitable reducing agent such as tin (II) chloride, iron, or hydrogen gas with a suitable catalyst such as palladium on carbon, in a suitable solvent such as methanol, ethanol or acetic acid, at a range of temperatures, preferably between room temperature and 100°C.
Compounds of general formula (le) are known in the literature or may be prepared by those skilled in the art using literature methods (e.g. WO2008034008, WO201 10189167, WO2010068258).
Alternatively, compounds of general formula (lea) as above defined can be prepared from compounds of formula (If), wherein R 17 , R 18 , z 1 , z 2 , z3 and z 4 are as defined above and wherein PG is a suitable compatible protecting group known to those skilled in the art, such as benzyl, benzyl carbamate or tert-butyl carbamate,
Figure imgf000061_0002
using suitable deprotection conditions such as hydrochloric acid, trifluoroacetic acid, or hydrogen catalysed by for example palladium on carbon, in a suitable solvent such as dichloromethane, methanol, ethanol or acetic acid, at a range of temperatures, preferably between 0°C and 100°C.
Compounds of general formula (If) can be prepared by reaction of compounds
17 18 1 2 3 4
of formula (lg), wherein z and z are as defined above
Figure imgf000062_0001
(1 g) (lh)
with compounds of formula (lh) as above reported wherein PG is a suitable protecting group known to those skilled in the art, such as benzyl, benzyl carbamate or tert-butyl carbamate, using suitable conditions such as in the presence of a base such as potassium carbonate or diisopropylethyl amine or under Buchwald conditions (with a catalyst such as Pd(OAc)2, a ligand such as 2,2'- bis(diphenylphosphino)-l , l'-binaphthyl and base such as sodium tert-butoxide), in a suitable solvent such as toluene or tetrahydrofuran, at a range of temperatures, preferably between room temperature and 150°C.
Compounds of general formula (lg) and (lh) are known in the literature or may be prepared by those skilled in the art by adapting appropriate literature methods (e.g. WO201 1042389, Chemistry-A European Journal, 201 1 , 17, 6606- 6609, S6606/1-S6606/38).
Compounds of general formula (la) may be prepared according to the route illustrated in Scheme 2.
Figure imgf000062_0002
Scheme 2
Compounds of general formula (la) may be prepared from compounds of general formula (2a) by reaction with a compound of general formula (2d). Wherein G is a suitable chemical group known to those skilled in the art selected such that it can facilitate a suitable coupling reaction such as nucleophilic displacement or metal catalysed cross coupling: For example in cases such that when Y is -0-, -S- or -NR7- , examples of G may include halogen or a suitable leaving group such as mesylate or triflate either directly linked or attached via a group -(CR3R4)n-. Examples of the coupling conditions used may include using a base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone in a suitable solvent such as Ν,Ν-dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C. For example in cases such that when Y is -O- and G is -ΟΗ or -SH a method to perform this coupling may involve Mitsunobu conditions (diethylazodicarboxylate / triphenylphosphine) in a suitable solvent such as tetrahydrofuran or 1 ,4-dioxane at a range of temperatures preferably between -10°C and 100°C. For example in cases such as when Y is -0-, -S- or -NR7- and G is a group such as halogen, triflate or boronic acid/ester a method to perform this coupling may be under metal (for example palladium or copper) catalysed coupling conditions in the presence of a suitable ligand such as Xantphos or 1 , 10- phenanthroline in the presence of a base such as caesium carbonate in a suitable solvent such as tetrahydrofuran, 1 ,4-dioxane or Ν,Ν-dimethylformamide at a range of temperatures preferably between - 10°C and 150°C. For example in cases such as when Y is -O- and G is a group such as -COOMe, -COOH, isocyanate, -OCOC1 or -NHCOOCH2CCI3 examples of conditions to perform this coupling may involve the use of a base such as sodium hydride or triethylamine or a coupling reagent such as HATU in a suitable solvent such as dichloromethane, tetrahydrofuran, 1 ,4-dioxane or Ν,Ν-dimethylformamide at a range of temperatures preferably between -10°C and 150°C.
Compounds of formula (2da), i.e. compounds of formula(2a) wherein X4 = C may be prepared according to the routes described in Scheme 3 herebelow:
Figure imgf000064_0001
(3e)
Scheme 3
Compounds of general formula (2da) as above defined may be prepared from compounds of general formula (3e) using a suitable oxidant such as chloramine T, lead tetracetate or phenyl iodine(III) diacetate, in a suitable solvent such as dichloromethane or ethanol at a range of temperatures, preferably between room temperature and 100°C.
Compounds of general formula (3e) may be prepared from compounds of general formula (3a) by reaction with an aldehyde of general formula (3c) above reported, in a suitable solvent such as ethanol or tetrahydrofuran at a range of temperatures, preferably between room temperature and 80°C.
Compounds of formula (3c) are commercially available, known in the literature or may be prepared using literature methods by those skilled in the art.
Alternatively, compounds of formula (2da) may be prepared from compounds of formula (3d) using a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazodicarboxylate/triphenylphosphine/trimethylsilylazide), in the absence or presence of a suitable solvent such as tetrahydrofuran, toluene or NMP, at a range of temperatures, preferably between room temperature and 150°C. Compounds of formula (3d) may be prepared from compounds of formula (3a) by reaction with a compound of general formula (3bl) using a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or acetonitrile, at a range of temperatures, preferably between room temperature and 150°C.
Or by reaction with a compound of general formula (3b2) in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or THF at a range of temperatures preferably between -10°C and the boiling point of the solvent.
Compounds of formulae (3b 1) and (3b2) are commercially available, known in the literature or may be prepared by literature methods by those skilled in the art.
Alternatively, compounds of formula (2da) as above defined may be prepared according to the route in Scheme 4:
Figure imgf000065_0001
Scheme 4
Compounds of general formula (2da) may be prepared from compounds of general formula (4c). Wherein G2 may represent groups such as halogen, -CHO,
-COOH, -COOEt and S02C1.
For example, compounds of general formula (2da) may be prepared from compounds of general formula (4c), wherein G2 represents halogen, using methods such as a metal (for example palladium) catalysed coupling with a suitable RnG5 derivative wherein G5 is a group such as boronate acid/ester or stannane in a suitable solvent such as tetrahydrofuran or 1 ,4-dioxane at a range of temperatures preferably between ambient temperature and 150°C. An alternative method may involve displacement of said halogen with a suitable group RnH (such as that containing an -NH, -OH or -SH group) in the presence of a base such as sodium hydride, potassium tert-butoxide or Ν,Ν-diethylisopropylamine in a suitable solvent such as Ν,Ν-dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
The group G2 may be also transformed from groups such as halogen to groups such as -CHO, -COOH, -COOEt and S02C1 by means of metal insertion methods known to those skilled in the art such as palladium catalysis, Grignard formation or lithium halogen exchange.
Compounds of general formula (2da) wherein R1 1 is a group such as -CH2-
NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)- NRARB, -C(0)N(Rc)-(C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3-C7cycloalkylene)-ORD, -C(0)N(RARB), -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3-
C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-ORD or -S(0)2N(Rc)-(C3- C7cycloalkylene)-ORD may be prepared from compounds of general formula (4c), wherein G2 represents -CHO, -COOH, -COOEt and -S02C1, by reaction with a suitable amine such as HNRARB etc using methods such as reductive amination (using a reagent such as sodium triacetoxyboro hydride) or amide/sulphonamide formation in the presence of suitable reagents such as HATU with a base such as Ν,Ν-diethylisopropylamine or trimethylaluminium in a suitable solvent such as dichloromethane, Ν,Ν-dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
Compounds of general formula (4c) wherein G2 is a group such as -COOEt, may be synthesised from compounds of general formula (4a) by reaction with a compound such as diethyloxalate in the presence of an acid such as acetic acid at a range of temperatures, preferably between room temperature and 120°C.
Compounds of general formula (4c) wherein G2 is a group such as bromine or chlorine, may be synthesised from compounds of general formula (4b) by reaction with a compound such as N-chlorosuccinimide or N-bromosuccinimide in a solvent such as chloroform at a range of temperatures, preferably between -10°C and room temperature.
Compounds of general formula (4b) may be synthesised from compounds of general formula (4a) by reaction with a compound such as diethoxymethylacetate at a range of temperatures, preferably between room temperature and 100°C.
Compounds of general formula (2db), i.e. compounds of formula (2d) wherein X4 is nitrogen, may be prepared from compounds of general formula (4b) wherein X4 = NH, by reaction with a suitable alkylating agent R1 1 in the presence of a base such as caesium carbonate in a suitable solvent such as Ν,Ν-dimethylformamide at a range of temperatures, preferably between room temperature and 150°C.
Alternatively, compounds of general formula (laa), i.e. compounds of formula (la) wherein X4 is CH may be prepared according to the route illustrated in Scheme 5.
Figure imgf000067_0001
(5d) (5e) (1aa)
Scheme 5 Compounds of general formula (laa) may be prepared from compounds of general formula (5e) wherein PG is a suitable protecting group known in the art such as Boc by using the appropriate deprotection conditions such as trifluoroacetic acid in a solvent such as dichloromethane at a range of temperatures, preferably between -10°C and room temperature.
Compounds of general formula (5e) may be prepared from compounds of general formula (5d) using a suitable dehydrating agent such as Burgess' reagent, triphenyl phosphine and hexachloroethane, phosphorus oxychloride, acetic acid or Mitsunobu conditions (diethylazadicarboxylate / triphenylphosphine / trimethylsilylazide), in the absence or presence of a suitable solvent such as tetrahydrofuran, toluene or NMP, at a range of temperatures, preferably between room temperature and 120°C.
Compounds of general formula (5d) may be prepared from compounds of general formula (5c) by reaction with a compound of general formula (3b 1) as above defined using a suitable acylating/dehydrating agent such as triphenylphosphine/trichloroacetonitrile in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or acetonitrile, at a range of temperatures, preferably between room temperature and 150°C.
or by reaction with a compound of general formula (3b2) as above defined in the presence of a base such as diisopropylethylamine, in a suitable solvent such as dichloromethane or THF at a range of temperatures preferably between - 10°C and the boiling point of the solvent.
Compounds of general formula (5c) may be prepared from compounds of general formula (5b) wherein G3 is a suitable leaving group such as halogen, by reaction with a reagent such as hydrazine mo no hydrate in a suitable solvent such as ethanol at a range of temperatures preferably between room temperature and 100°C.
Compounds of general formula (5b) may be prepared from compounds of general formula (5a) by reaction with a suitable protecting group reagent known in the art such as Boc anhydride in the presence of a base such as triethylamine in a suitable solvent such as dichloromethane or tetrahydrofuran at a range of temperatures preferably between room temperature and 100°C.
Compounds of general formula (5a) can be synthesised by the methods described above for the synthesis of (l a).
Compounds of general formula (2aa), i.e. compounds of formula (2a) wherein Y = 0,W = NH and PG is a suitable protective group such as trifluoroacetate may be prepared according to the route illustrated in scheme 6:
Figure imgf000069_0001
(6c)
Scheme 6
Compounds of general formula (2aa) may be prepared from compounds of general formula (6b) and (6c) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol at a range of temperatures preferably between room temperature and 100°C.
Compounds of general formula (6b), wherein PG is a protecting group, preferably trifluoroacetamide, and the group -OH is placed on the cycloalkylene portion of ring A may be prepared from compounds of general formula (6a) by using a chiral reductive method such as using formic acid and RuCl[S,S-Tsdpen(p- cymene)] in the presence of a base such as triethylamine in a solvent such as N,N- dimethylformamide at a range of temperatures preferably between room temperature and 150°C. It will be recognised that compounds of formula (6a) may be homochiral as illustrated or be the opposite enantiomer or racemic.
It will be realised by those skilled in the art that any combination of stereocentres in (2aa) can be prepared using both enantiomers of (6a) and using RuCl[R,R-Tsdpen(p-cymene)] or RuCl[S,S-Tsdpen(p-cymene)]. Compound (2a) is drawn with no defined stereocentres but any combination can be obtained as illustrated in Scheme 2.
Compounds of formula (6a) can be prepared from compounds of formula (6d)
Figure imgf000070_0001
using a suitable oxidant such as potassium permanganate and magnesium sulfate in a suitable solvent methanol/water at a range of temperatures preferably between room temperature and the boiling point of the solvent. It will be recognised that compounds of formula (6d) may be homochiral as illustrated or be the opposite enantiomer or racemic.
Compounds of formula (6d) can be prepared from compounds of formula (6e) where PG is a suitable protecting group such as trifluoro acetate or tert-butyl carbonate:
Figure imgf000070_0002
using ethyl trifluoroacetate or di-tert-butyl dicarbonate in the presence of base such as triethylamine or diisopropylethylamine in a solvent such as methanol or dichloromethane at a range of temperatures preferably between 0°C and the boiling point of the solvent. It will be recognised that compounds of formula (6e) may be homochiral as illustrated or be the opposite enantiomer or racemic.
Compounds of formula (6e) are known in the literature and may be prepared by those skilled in the art by adapting literature methods (e.g. for S-(+)-l-amino- 1 ,2,3,4-tetrahydronaphthalene see Journal of the Chemical Society, Perkin Transactions 1 : 1985, 2039-44, for (S)-(+)-8-amino-5,6,7,8-tetrahydroquinoline see Journal of Organic Chemistry, 2007, 72, 669-671 and for 1-aminoindan see Tetrahedron Letters, 201 1 , 52, 1310-1312).
Compounds of general formula (2ab), i.e. compounds of formula (2a) wherein Y = NR7 and W = NH, may be prepared according to the route illustrated in scheme
Figure imgf000071_0001
(6c) (7c)
Scheme 7
Compounds of general formula (2ab) may be prepared from compounds of general formula (7a) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol or trifluoroacetic acid in dichloromethane at a range of temperatures preferably between room temperature and 100°C.
Compounds of formula (7a) may be prepared from compounds of general formula (6a) and amines (7b) by reaction under reductive amination conditions, using a reducing agent such as sodium triacetoxyboro hydride and a solvent such as 1 ,2-dichloroethane at a range of temperatures preferably between room temperature and 100°C.
Compounds for formula (7b) are known in the literature and may be prepared by those skilled in the art using literature procedures. Compounds of formula (6a) can be prepared as described above. Alternatively, compounds of formula (7a) may be prepared from compounds of general formula (7c) wherein G4 is a suitable chemical group known to those skilled in the art selected such that it can facilitate a reaction such as a nucleophilic substitution. For example G is a suitable leaving group such as halogen or mesylate which can react with a suitable amine (7b) in the presence of a suitable base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro- 2(lH)-pyrimidinone in a suitable solvent such as Ν,Ν-dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C.
Compounds of formula (7c) can be prepared from compounds of formulae (6b) or (6c) using halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane or activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropy lamine .
Alternatively, compounds of general formula (Id), i.e. compounds of formula (I) wherein Y = S and W = ΝΗ may be prepared according to the route illustrated in scheme 8:
Figure imgf000072_0001
Figure imgf000072_0002
Compounds of general formula (Id) may be prepared from compounds of general formula (lab), i.e. compounds of formula (la) wherein Y = S and W = ΝΗ: using compounds of formula (lb 1) or (lb2) in a suitable solvent such as dimethyl sulfoxide, 1 ,4-dioxane, Ν,Ν-dimethylformamide or acetonitrile, in the presence of a base such as diisopropylethylamme at a range of temperatures, preferably between room temperature and 100°C.
Compounds of formula (lab) as above defined may be prepared from compounds of formula (8g) using deprotection conditions such as hydrazine in methanol at a range of temperatures preferably between room temperature and the boiling point of the solvent.
Compounds of formula (8g) wherein Y = S, can be prepared from compounds of formula (8f) by reaction with compounds of formulae (2b), (2c) or (2d). Examples of the coupling conditions used may include using a base such as sodium hydride or potassium tert-butoxide and l ,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone in a suitable solvent such as Ν,Ν-dimethylformamide, toluene, 1 ,4-dioxane or acetonitrile at a range of temperatures, preferably between room temperature and 150°C. Alternative methods to perform this coupling may involve Mitsunobu conditions (diethylazodicarboxylate / triphenylphosphine) or metal (for example palladium) catalysed coupling conditions in a suitable solvent such as tetrahydrofuran or 1 ,4-dioxane at a range of temperatures preferably between -10°C and 150°C and starting from the appropriate derivative of formula (2b), (2c), or (2d).
Compounds of formula (8f) can be prepared from compounds of formula (8e) using dithiothreitol, monopotassium phosphate, potassium carbonate in a solvent such as methanol in the presence of acetic acid at a range of temperatures preferably between room temperature and the boiling point of the solvent.
Compounds of formula (8e) can be prepared from compounds of formula (8d) using 2-nitrobenzenesulfenyl chloride in acetic acid at a range of temperatures preferably between room temperature and 100°C.
Compounds of formula (8d) can be prepared from compounds of formula (8c) using phthalimide, triphenylphosphine and diisopropyl azodicarboxylate in a solvent such as tetrahydrofuran at a range of temperature preferably between 0°C and the boiling point of the solvent.
Compounds of formula (8c) can be prepared from compounds of formula (8b) using a reducing agent such as sodium boro hydride in a solvent such as methanol at a range of temperatures preferably between 0°C and the boiling point of the solvent.
Compounds of formula (8b) can be prepared from compounds of formula (8a) using tert-butanethiol in the presence of a base such as diisopropylethylamine in a solvent such as tetrahydrofuran at a range of temperatures preferably between 0°C and the boiling point of the solvent.
Compounds of formula (8a) are known in the literature and can be prepared by those skilled in the art using literature methods (e.g. 3-bromo-indan- l-one see WO 2010108058).
Alternatively, compounds of general formula (lab), i.e. compounds of formula (la) wherein Y = CH2, W = NH and PG is a suitable protective group such as trifluoro acetate may be prepared according to the route illustrated in scheme 9:
Figure imgf000074_0001
(1 ab)
Scheme 9
Compounds of general formula (lab) may be prepared from compounds of general formula (9b) by removal of the protecting group PG using methods known in the art such as aqueous sodium hydroxide in a solvent such as methanol at a range of temperatures preferably between room temperature and 100°C.
Compounds of formula (9b) may be prepared from compounds of general formula (9a) by reaction with a suitable reduction agent for example hydrogen gas in the presence of a suitable catalyst such as palladium on activated charcoal in a suitable solvent such as ethanol at a range of temperatures between room temperature and 70°C and pressures between atmospheric and 4 Barr.
Compounds of formula (9a) may be prepared from compounds of general formula (6a) by means of a reaction such as a Wittig (or one of the closely related variants such as the Horner- Wadsworth-Emmons) with a suitable substrate such as R1-CH2-P(0)(OMe)2 in the presence of a suitable base such as sodium hydride in a suitable solvent such as tetrahydrofuran at a range of temperatures preferably between -10°C and 100°C.
Compounds such as R1-CH2-P(0)(OMe)2 may be synthesised from compounds of the general formula R1-CH2-Hal wherein Hal represents a halogen such as -Br or -CI by reaction with a compound such as trimethylphosphite at a range of temperatures preferably between 0°C and 100°C.
Compounds such as R1-CH2-Hal may be synthesised from compounds of formula R'-CHs by means of a reaction such as a radial halogenation using a reagent such as N-bromosuccinimide in the presence of a catalyst such as AIBN in a suitable solvent such as carbon tetrachloride at a range of temperatures preferably between 0°C and 80°C. Compounds such as R1-CH2-Hal may also be synthesised from compounds formula R1-CH2-OH by means of using halogenating conditions such as carbon tetrabromide and triphenylphosphine in dichloromethane or activation conditions such as methane sulfonyl chloride in dichloromethane in the presence of base such as diisopropylamine.
Compounds such as R'-CHs and R1-CH2-OH may be prepared by methods outlined above for compounds (2b), (2c) and (2d).
Compounds of the invention of formula (l ac), i.e. compounds of formula (l a) where Y = (CR5R6)n and W = NH, may be prepared according to the route illustrated in Scheme 10.
R1— Y-B(OR), 2
OTf (10f)
(6a) PG-W- ^ PG-W^
(10a) (10b)
Figure imgf000076_0001
(10c) (1 ac) Scheme 10
Compounds of formula (lac) may be prepared from compounds of formula (10c) wherein PG is a suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using suitable de-protection conditions such as, sodium hydroxide in methanol, trifluoroacetic acid in dichloromethane or hydrogen gas catalysed by for example palladium on carbon in ethanol, at a range of temperatures, preferably between 0°C and 100°C.
Compounds of formula (10c) may be prepared from compounds of formula (10b) wherein PG is a suitable protecting group known to those skilled in the art, such as trifluoroacetamide, tert-butyl carbamate and benzyl carbamate by using hydrogen gas in the presence of a catalyst such as palladium on carbon, in a suitable solvent such as methanol or ethanol, in the presence or absence of an acid such as HC1, at a range of temperatures, preferably between 0°C and 100°C.
Compounds of formula (10b) may be prepared from compounds of formula (10a) and (lOf) by a reaction such as a cross-coupling using a suitable catalyst such as tetrakis(triphenylphosphine)palladium (0) or palladium acetate, and a base such as diisopropylethylamine, sodium tert-butoxide or caesium carbonate in a suitable solvent such as NMP, toluene or DMF, at a range of temperatures, preferably between 0°C and 100°C. Alternatively (10b) may be prepared by adapting literature procedures (e.g those reported in WO2009022633).
Compounds of formula (lOf) are known in the literature or may be prepared by those skilled in the art by adapting literature procedures (e.g WO2008063287).
Compounds of formula (10a) may be prepared from compounds of formula (6a) using a inflating agent such as triflic anhydride, in the presence of a suitable base such as pyridine or 2,6-bis(tert-butyl)-4-methylpyridine, in a solvent such as dichloromethane or chloroform at a range of temperatures, preferably between 0°C and boiling point of the solvent. Alternatively (10a) may be prepared by adapting literature procedures (e.g those described in WO2009022633).
General experimental details
Abbreviations used in the experimental section: AcOH = acetic acid; aq. = aqueous; DCM = dichloromethane; DIAD = Diisopropyl azodicarboxylate; DIPEA = diisopropylethylamine; DMAP = N,N- dimethylaminopyridine; DMF = N,N- dimethylformamide; DMSO = dimethyl sulfoxide; EDC = l-ethyl-3-(3'- dimethylaminopropyl)carbodiimide Hydrochloride; EtOAc = ethyl acetate; EtOH = ethanol; Et20 = diethyl ether; EtsN = triethylamine; EtNiPr2 = diisopropylethylamine; FCC = flash column chromatography; h = hour; HATU = 2- (7-aza- 1 H-benzotriazole- 1 -yl)- 1 , 1 ,3 ,3-tetramethyluronium hexafluorophosphate; HOBt = 1 -hydro xy-benzotriazole; HPLC = high performance liquid chromatography; IMS = Industrial Methylated Spirits; LCMS = liquid chromatography mass spectrometry; NaOH = sodium hydroxide; MeCN = acetonitrile; MeOH = Methanol; min = minutes; NH3 = ammonia; NMR = nuclear magnetic resonance; RT = room temperature; Rt = retention time; sat. = saturated; SCX-2 = strong cation exchange chromatography; TFA = trifluoroacetic acid; THF = Tetrahydrofuran; H20 = water; Xantphos = 4,5-Bis(diphenylphosphino)-9,9- dimethylxanthene; X-Select = Waters X-select HPLC column; IPA = propan-2-ol; LDA = lithium diisopropylamide; MDAP = mass-directed auto-purification; Ρ1¾Ρ = triphenylphosphine; TBAF = tetrabutylammonium fluoride.
In the procedures that follow, after each starting material, reference to a
Intermediate/Example number is usually provided. This is provided merely for assistance to the skilled chemist. The starting material may not necessarily have been prepared from the batch referred to.
When reference is made to the use of a "similar" or "analogous" procedure, as will be appreciated by those skilled in the art, such a procedure may involve minor variations, for example reaction temperature, reagent/solvent amount, reaction time, work-up conditions or chromatographic purification conditions.
The nomenclature of structures was assigned using Autonom 2000 Name software from MDL Inc. When the nomenclature of structures could not be assigned using Autonom, ACD/Name software utility part of the ACD/Labs Release 12.00 Product Version 12.5 (Build 45133, 16 Dec 2010) was used. Stereochemical assignments of compounds are based on comparisons with data reported in WO2008/043019 for key intermediates. All reactions were carried out under anhydrous conditions and an atmosphere of nitrogen or argon unless specified otherwise. Unless otherwise stated all transformations were carried at ambient temperature (room temperature).
NMR spectra were obtained on a Varian Unity Inova 400 spectrometer with a 5mm inverse detection triple resonance probe operating at 400 MHz or on a Bruker Avance DRX 400 spectrometer with a 5 mm inverse detection triple resonance TXI probe operating at 400 MHz or on a Bruker Avance DPX 300 spectrometer with a standard 5mm dual frequency probe operating at 300 MHz. Shifts are given in ppm relative to tetramethylsilane (δ = 0 ppm). J values are given in Hz through-out. NMR spectra were assigned using DataChord Spectrum Analyst Version 4.0.b21 or SpinWorks version 3.
Where products were purified by flash column chromatography (FCC), 'flash silica' refers to silica gel for chromatography, 0.035 to 0.070 mm (220 to 440 mesh) (e.g. Fluka silica gel 60), and an applied pressure of nitrogen up to 10 p.s.i accelerated column elution or use of the CombiFlash® Companion purification system or use of the Biotage SP l purification system. All solvents and commercial reagents were used as received.
Compounds purified by preparative HPLC were purified using a C I S-reverse- phase column (100 x 22.5 mm i.d Genesis column with 7 μιη particle size), or a Phenyl-Hexyl column (250 x 21.2 mm i.d. Gemini column with 5 μιη particle size), UV detection between 220 - 254 nm, flow 5-20 mL/min), eluting with gradients from 100-0 to 0-100% water/acetonitrile (containing 0.1% TFA or 0.1% formic acid) or water/MeOH (containing 0.1 % TFA or 0.1% formic acid), or a C18-reverse-phase column (19 x 250 mm, XBridge OBD, with 5 μιη particle size), eluting with gradients from 100-0 to 0-100% water/acetonitrile (containing 0.1% NH4OH); or a ChiralPak IC column (10 x 250 mm i.d., with 5 μιη particle size), unless otherwise indicated. Fractions containing the required product (identified by LCMS analysis) were pooled, the organic solvent removed by evaporation, and the remaining aqueous residue lyophilised, to give the final product. Products purified by preparative HPLC were isolated as free base, formate or TFA salts, unless otherwise stated.
The Liquid Chromatography Mass Spectroscopy (LCMS) and HPLC systems used are:
Method 1
Waters Platform LC Quadrupole mass spectrometer with a C 18-reverse-phase column (30 x 4.6 mm Phenomenex Luna 3 μιη particle size), elution with A: water + 0.1 % formic acid; B: acetonitrile + 0.1% formic acid. Gradient: Gradient - Time flow mL/min %A %B
0.00 2.0 95 5
0.50 2.0 95 5
4.50 2.0 5 95
5.50 2.0 5 95
6.00 2.0 95 5
Detection - MS, ELS, UV (200 split to MS with in-line HPl l OO DAD detector). MS ionization method - Electrospray (positive and negative ion).
Method 2
Waters ZMD quadrupole mass spectrometer with a C18-reverse-phase column (30 x 4.6 mm Phenomenex Luna 3 μιη particle size), elution with A: water + 0.1 % formic acid; B: acetonitrile + 0.1% formic acid. Gradient:
Gradient - Time flow mL/min %A %B
0.00 2.0 95 5
0.50 2.0 95 5
4.50 2.0 5 95
5.50 2.0 5 95
6.00 2.0 95 5
Detection - MS, ELS, UV (200 split to MS with in-line Waters 996 DAD detector). MS ionization method - Electrospray (positive and negative ion).
Method 3
Waters ZMD quadrupole mass spectrometer with a C18-reverse-phase column (30 x 4.6 mm Phenomenex Luna 3 μιη particle size), elution with A: water + 0.1 % formic acid; B: acetonitrile + 0.1% formic acid. Gradient: Gradient - Time flow mL/min %A %B
0.00 2.0 95 5
0.50 2.0 95 5
4.50 2.0 5 95
5.50 2.0 5 95
6.00 2.0 95 5
Detection - MS, ELS, UV (200 μΕ split to MS with in-line HP1 100 DAD detector). MS ionization method - Electrospray (positive and negative ion).
Method 4
VG Platform II quadrupole spectrometer with a C18-reverse-phase column (30 x 4.6 mm Phenomenex Luna 3 μιη particle size, elution with A: water + 0.1% formic acid; B: acetonitrile + 0.1% formic acid. Gradient:
Gradient - Time flow mL/min %A %B
0.00 2.0 95 5
0.30 2.0 95 5
4.30 2.0 5 95
5.30 2.0 5 95
5.80 2.0 95 5
6.00 2.0 95 5
Detection - MS, ELS, UV (200 μΐ/min split to the ESI source with inline
HP 1050 DAD detector). MS ionization method - Electrospray (positive and negative ion).
Method 5
Waters micromass ZQ2000 quadrupole mass spectrometer with an Acquity BEH C 18 1.7um 100 x 2.1mm, Acquity BEH Shield RP18 1.7um 100 x 2.1mm or Acquity HSST3 1.8um 100 x 2.1mm, maintained at 40°C. Elution with A: water + 0.1 % formic acid; B: acetonitrile + 0.1% formic acid. Gradient: Gradient - Time flow mL/min %A %B
0.00 0.4 95 5
0.40 0.4 95 5
6.00 0.4 5 95
6.80 0.4 5 95
7.00 0.4 95 5
8.00 0.4 95 5
Detection - MS, UV PDA. MS ionization method - Electrospray (positive and negative ion).
Method 6
Phenomenex Gemini C 18-reverse-phase column (250 x 21.20 mm 5 μιη particle size), elution with A: water + 0.1% formic acid; B: C¾CN + 0.1% formic acid. Gradient - 90% A/10% B to 2% A/98% B over 20 min - flow rate 18 mL/min. Detection - In-line UV detector set at 254 nM wavelength.
Method 7
Agilent 1260 infinity purification system. Column: XSELECT CSH Prep C 18 OBD, particle size 5μιη, 30 x 150mm, RT. Elution with A: water + 0.1% formic acid; B: CH3CN + 0.1% formic acid. Gradient - 90% A/10% B to 2% A/95% B over 22 min - flow rate 60 mL/min. Detection - In-line Agilent 6100 series single Quadrupole LC/MS.
Method 8
Agilent 1260 infinity purification system. Column: XBridge Prep C 18 OBD, particle size 5μιη, 30 x 150mm, RT. Elution with A: water + 0.1% ammonia; B: CH3CN + 0.1% ammonia. Gradient - 90% A/10% B to 2% A/95% B over 22 min - flow rate 60 mL/min. Detection - In-line Agilent 6100 series single Quadrupole LC/MS.
Intermediate 1
(lR,4S)-4-Amino-l,2,3,4-tetrahydro-naphthalen-l-ol
Figure imgf000083_0001
a. 2,2,2-Trifluoro- V-(S)-l,2,3,4-tetrahydro-naphthalen-l-yl- acetamide (Intermediate la)
Figure imgf000083_0002
To a mechanically stirred solution of (£)-(+)-( 1 ,2,3, 4-tetrahydro-naphthalen- l-yl)amine (CAS: 23357-52-0, 175 g, 1.19 mol) and triethylamine (250 mL, 1.79 mol) in MeOH (1.75 L), ethyl trifluoroacetate (170 mL, 1.43 mol) was added dropwise at a rate to maintain the internal temperature below 30°C (ca. over 20 min). The resulting solution was stirred at RT overnight. The mixture was concentrated in vacuo to give a solid. This was partitioned between DCM (1 L) and water (1 L). The layers were separated and the aqueous layer was extracted with DCM (2 x 600 mL). The combined organic layers were washed with brine, dried (Na2S04), filtered and concentrated in vacuo to yield Intermediate la (289.4 g, 100%). Ή NMR (400 MHz, CDC13): 1.80-1.95 (3H, m), 2.05-2.15 (1H, m), 2.75- 2.90 (2H, m), 5.18-5.25 (1H, q, J = 5.0 Hz), 6.38-6.48 (1H, br s), 7.12-7.16 (1H, m), 7.20-7.26 (3H, m).
b. 2,2,2-Trifluoro- V-((S)-4-oxo-l,2,3,4-tetrahydro-naphthalen-l-yl)- acetamide (Intermediate lb)
Figure imgf000083_0003
A 20 L flask was charged with Intermediate la (288 g, 1.19 mol) and acetone (7 L). Magnesium sulfate monohydrate (328 g, 2.37 mol) in water (3 L) was added and the mixture stirred mechanically, and cooled to internal temperature ~1.5°C. Potassium permanganate (562.1 g, 3.56 mol) was then added in 7 equal portions (i.e. 80.3 g) every 15 min for 105 min. Water (0.5 L) was added and the resulting mixture was stirred at RT for 17 h. The mixture was cooled to 15°C and a solution of sodium thiosulfate pentahydrate (883 g, 3.56 mol) in water (3 L) was added dropwise over 1 h, whilst maintaining internal temperature below 18°C. The resulting slurry was stirred for 1 h and the mixture left to stand at RT overnight. Solid had settled at the bottom of the flask and the solution was decanted and then concentrated to leave a residue. The remaining solid was treated with ethyl acetate (7 L) and water (2 L) and the mixture was filtered through Celite. The filtrate was combined with the residue isolated above. The mixture was separated and the aqueous layer extracted with ethyl acetate (2 x 1 L). The organics were combined and drying agent (Na2S04) and decolourising charcoal were added. The mixture was filtered through Celite and concentrated to dryness in vacuo to yield Intermediate lb (260 g, 85%). Ή NMR (400 MHz, CDC13): 2.20-2.30 (1H, dddd, J = 13.3, 10.0, 8.8, 4.5 Hz), 2.43-2.52 (1H, dddd, J = 13.3, 7.2, 4.6, 4.6 Hz), 2.67-2.77 (1H, ddd, J = 17.4, 10.1 , 4.6 Hz), 2.78-2.88 (1H, ddd, J = 17.4, 7.1 , 4.6 Hz), 5.39-5.47 (1H, td, J = 8.5, 4.5 Hz), 7.32-7.37 (1H, d, J = 7.7 Hz), 7.44-7.49 (1H, t, J = 7.6 Hz), 7.59-7.64 (1H, td, J = 7.6, 1.4 Hz), 8.03-8.07 (1H, dd, J = 7.7, 1.4 Hz).
c. 2,2,2-Trifluoro- V-((lS,4R)-4-hydroxy-l,2,3,4-tetrahydro- naphthalen-l-yl)-acetamide (Intermediate lc)
Figure imgf000084_0001
A solution of Intermediate lb (161 g, 624 mmol) in DMF (2 L) was vacuum degassed with argon. [N-[(lR,2R)-2-(Amino-KN)-l ,2-diphenylethyl]-4- methylbenzenesulfonamidato-KN]chloro[(l ,2,3,4,5,6^)-l-methyl-4-(l- methylethyl)benzene] -ruthenium (CAS: 192139-92-7, 9.95 g, 15.6 mmol) was then added. Formic acid (57.5 g, 1.25 mol) was added slowly to ice cold triethylamine (126 g, 1.25 mol) with stirring, this was then added to the DMF solution. The resulting reaction mixture was heated to 50°C (internal temperature) for 41 h with stirring. LCMS analysis of the reaction indicated it was incomplete, therefore a solution of formic acid (14.4 g, 313 mmol) was added slowly to ice cold triethylamine (31.6 g, 312 mmol), this was then added to the reaction mixture. Heating was continued for an additional 22 h. After cooling, the mixture was concentrated in vacuo to give an orange residue. The residue was diluted with ethyl acetate (1.5 L) and the solution washed with brine (2 x 0.5 L). The organics were dried (Na2S04), filtered and concentrated in vacuo. Purification by column chromatography (Silica, 3 Kg, 0-50% ethyl acetate in cyclohexane) gave Intermediate lc (1 18 g, 73%). 97.5 d.e.% determination by LCMS Rt 3.37 min, M- H 258 (93.7%, desired); Rt 3.25 min, M-H 258 (1.2%, trans isomer). Ή NMR (400 MHz, CDCls): 1.88-1.92 (1H, d, J = 4.8 Hz), 1.98-2.18 (4H, m), 4.80-4.88 (1H, m), 5.165-5.24 (1H, m), 6.70-6.80 (1H, br s), 7.25-7.30 (1H, m), 7.30-7.40 (2H, m), 7.45-7.50 (1H, m).
d. (lR,4S)-4-Amino-l,2,3,4-tetrahydro-naphthalen-l-ol (Intermediate
Figure imgf000085_0001
To a stirred solution of Intermediate lc (1 17 g, 451 mmol) in methanol (0.7 L), 6N aqueous sodium hydroxide solution (190 mL, 1.14 mol) was added and stirred at RT for 20 h. The mixture was concentrated in vacuo and the residue was diluted with ethyl acetate (1 L) and water (0.5 L). Concentrated HCl solution (95 mL, 1.14 mol) was added slowly with stirring. Additional HCl was used to adjust the pH of the aqueous layer to pH = 2. The mixture was then separated and the organic layer was extracted with HCl solution (2M aqueous, 3 x 500 mL). The combined aqueous layers were basified to pH ~ 12, by addition of concentrated aqueous NH4OH solution, and then extracted in to ethyl acetate (5 x 750 mL). The combined organic extracts were washed with brine (200 mL), dried (Na2S04), filtered and concentrated in vacuo to give a solid (50.8 g). This material was recrystallized (cyclohexane/ethyl acetate [2: 1], 350 mL) to provide Intermediate 1 (44.4 g, 60%>). Ή NMR (400 MHz, d6-DMSO): 1.66-1.90 (4H, m), 3.71-3.77 (1H, t, J = 5.4 Hz), 4.46-4.54 (1H, t, J = 5.4 Hz), 7.14-7.22 (2H, m), 7.32-7.38 (1H, m), 7.40-7.46 (1H, m).
Intermediate 2
(lS,4R)-4-[3-((S)-2-Methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-ylamine
Figure imgf000086_0001
a. (R)-Hydroxy-phenyl-acetate(S)-2-methyl-piperidinium
(Intermediate 2a)
Figure imgf000086_0002
2-Methylpiperidine (99.7 g, 1.0 mol) [CAS 109-05-7] was dissolved in MeOH (100 mL) in a 2L Florentine flask and cooled in an ice bath. (R)-(-)-mandelic acid (152.9 g, 1.0 mol) [CAS 61 1-71-2] was then added and the reagents stirred with gentle heating until a homogenous solution resulted. The solution was left to cool, and Et20 (900 mL) was added. The flask walls were scratched to aid crystallisation, and then stored in a fridge for 18h. The resulting crystals were then filtered off, and washed with cold Et20. The product was recrystallized again from MeOH (100 mL) and Et20 (500 mL) and left in a fridge for 48 h. The crystals were filtered off, washed with Et20 and dried in a vacuum oven at 50°C overnight to afford Intermediate 2a (66.97 g, 53%) as colourless crystals. Ή NMR (300 MHz, d6-
DMSO-dg): 1.12 (3H, d, J = 6.5 Hz), 1.20-1.57 (3H, m), 1.58-1.74 (3H, m), 2.72 (1H, dt, J = 3.2, 12.4 Hz), 2.88-3.02 (1H, m), 3.06-3.18 (1H, m), 4.51 (1H, s), 7.1 1- 7.19 (1H, m), 7.19-7.29 (2H, m), 7.33-7.42 (2H, m).
Diastereomeric purity was measured using Marfey's method; compound 2 (1 mg, 3.68 μηιοΐ) was dissolved in EtOAc (1 mL) and H20 (1 mL) and Marfeys reagent was added (Na-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, FDAA [CAS 95713-52-3] , 1 mg, 3.68 μιηοΐ) followed by saturated NaHC03 solution (50 μί) and heated to 50°C for 1 hour. The mixture was then diluted with H20 (1 mL) and subjected to analytical HPLC (Waters X-Select C I 8, 2.5 μιη, 4.6 x 50 mm, 32-34% CH3CN/H20 (+0.1 % formic acid), 16 min gradient, 1 mL/min, 340 nm). Rt 10.82 min, >99% d.e.
Racemic 2-methylpiperidine was also subjected to Marfey's method; HPLC : Rt 10.75 min (50%), 1 1 .581 min (50%).
b. (S)-2-Methyl-piperidine-l-carbonyl chloride (Intermediate 2b)
Figure imgf000087_0001
Intermediate 2a (12.0 g, 47.75 mmol) was treated with aqueous NaOH solution (IN; 96 mL, 96 mmol) and extracted into DCM (2 x 75 mL). This solution of (iS)-2-methyl piperidine was transferred to a 3-necked RB flask, stirred under an inert atmosphere and cooled in an ice-bath before pyridine (1 1.6 mL, 143.72 mmol) was added followed by triphosgene (14.17 g, 47.75 mmol) during 30 min at < 10°C. The cooling bath was removed after 30 min and the mixture stirred at RT for a further 3.5 hours. Reaction was quenched by very careful addition of aqueous HC1 (IN, 300 mL) at 0-5°C. After 30 min the phases were separated and the aqueous layer extracted with DCM (2 x 100 mL). Combined DCM extracts were washed with brine, dried (MgS04), passed through a phase separation cartridge and concentrated in vacuo to give Intermediate 2b (8.6 g, >100%> still containing some DCM). Ή NMR (300 MHz, CDC13): 1.25 (3H, d, J 6.8), 1.40- 1.80 (6H, m), 3.0 (1 H, br), 4.12- 4.21 (1H, m), 4.56-4.67 (1H, m).
c. (S)-2-Methyl-piperidine-l-carboxylic acid V'-(5-fluoro-pyridin-2- yl)-hydrazide (Intermediate 2c)
Figure imgf000088_0001
A stirred solution of Intermediate 2b (17.20 g, assumed to be 95.49 mmol) and (5-fluoro-pyridin-2-yl)-hydrazine (12.14 g, 95.51 mmol) in DCM (300 mL) at RT was treated with DIPEA (34 mL, 195.18 mmol) during 5 min. This mixture was continued to be stirred at RT for 4 days before being added to water (500 mL) and phases separated. The aqueous layer was further extracted into DCM (4 x 100 mL), combined extracts washed with brine, dried (MgS04), passed through a phase separation cartridge and concentrated in vacuo to give a residual solid. This product was treated with Et20 - pentane and the resultant solids filtered off and dried to give Intermediate 2c (18.74 g, 77%). LCMS (Method 3) Rt 2.26 min, m/z 253 [MH+] . Ή NMR (300 MHz, CDC13): 1.23 (3H, d, J 6.9), 1.40- 1.74 (6H, m), 2.97 (1H, td, J 13.1 , 3.0), 3.82-3.91 (1H, m), 4.27-4.38 (1H, m), 6.54 (1H, s), 6.78 (1H, ddd, J 9.1 , 3.7, 0.6), 7.30 (1H, ddd, J 9.1 , 7.8, 2.9), 8.00 (1 H, d, J 2.6).
d. 6-Fluoro-3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a] pyridine (Intermediate 2d)
Figure imgf000088_0002
To a stirred solution of Intermediate 2c (14.70 g, 58.27 mmol), Ph3P (30.56 g, 1 16.51 mmol) and Et3N (33 mL, 236.76 mmol) in THF (300 mL) at RT was added hexachloro ethane (27.60 g, 1 16.58 mmol) during 10 min before then heating at 60°C overnight. The cooled mixture was filtered and concentrated in vacuo to give a residual oil which was dissolved in DCM (200 mL) and extracted into dilute HC1 (2M) until most product had been removed from the DCM phase by LCMS. These aqueous extracts were treated with solid NaOH (with cooling) to achieve ~pH9 and extracted into DCM. Combined DCM extracts were washed with brine, dried (MgS04), passed through a phase separation cartridge and concentrated in vacuo to give Intermediate 2d (1 1.30 g, 82%). LCMS (Method 3) Rt 2.99 min, m/z 235 [MH+]. Ή NMR (300 MHz, d6-d6-DMSO): 0.89 (3H, d, J 6.3), 1.40-1.88 (6H, m), 2.85-2.96 (1H, m), 3.18 (1H, dt, J 12.0, 4.5), 3.28-3.35 (1H, m), 7.42 (1H, ddd, J 10.0, 8.0, 2.3), 7.76 (1H, ddd, J 10.0, 4.9, 0.9), 8.31-8.35 (1H, m).
e. (lS,4R)-4-[3-((S)-2-Methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-ylamine (Intermediate 2)
Sodium hydride (60% dispersion in oil, 12 g, 300 mmol) was suspended in DMF (800 mL) and cooled to 0°C using an ice bath. (24.45 g, 150 mmol) was then added in small portions under N2 and the resulting opaque brown suspension was stirred at RT for 45 min (CARE: gas evolution). A solution of Intermediate 2d (35.1 g, 150 mmol) in dry DMF (200 mL) was added and the solution stirred at RT for 18 h. The solution was concentrated in vacuo, the residue was poured into a mixture of brine/lN aqueous NaOH /H20 (1 : 1 : 1 ; 200 mL); the product was extracted using mixture of EtOAc and Me-THF (300 mL X 5). The organic extracts were combined, washed with a small amount of brine, dried over MgS04 and concentrated under reduced pressure. The product was purified by FCC, eluting with 0-20%> [2M NH3 in MeOH] in DCM, to provide the title compound (27 A g, 48%) as a pale brown foam. LCMS (Method 3): Rt 2.29 min, m/z 378 [MH+].
Intermediate 3
(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-ylamine
Figure imgf000089_0001
a. (2S,6R)-2,6-Dimethyl-piperidine-l-carbonyl chloride (Intermediate
3a)
Figure imgf000090_0001
To a solution of triphosgene (20.8 g, 70.0 mmol) in DCM (400 mL) at 5°C was added pyridine (16.2 mL, 200 mmol) dropwise over 10 min, maintaining the temperature below 10°C. The solution was then stirred between 5-10°C for 1 h, then cis-2,6-dimethyl piperidine (CAS: 766-17-6, 27.0 mL, 200 mmol) was added dropwise over 10 min and the resulting red solution stirred at RT for 4 days (reaction complete within <4 h). The solution was cooled to 3°C, then a pre-cooled (3°C) 1M aqueous HC1 solution (400 mL) was added and the mixture stirred at 5°C for 30 min. The mixture was separated and the aqueous layer was extracted with
DCM (200 mL), then the combined organic extracts passed through a hydrophobic frit and concentrated under vacuum affording Intermediate 3a as a red oil (31.5 g, 90%). Ή NMR (300 MHz, CDC13): 1.30 (6H, d, J = 7.09 Hz), 1.49-1.87 (6H, m), 4.46-4.56 (2H, m).
b. (2S,6R)-2,6-Dimethyl-piperidine-l-carboxylic acid N'-(5-fluoro- pyridin-2-yl)-hydrazide (Intermediate 3b)
Figure imgf000090_0002
A solution of (5-fluoro-pyridine-2-yl)-hydrazine (21.7 g, 171 mmol), Intermediate 3a (31.5 g, 180 mmol) and DIPEA (44.7 mmol, 256 mmol) in DCM (350 mL) was stirred at RT for 4 days. Water (350 mL) was added, then the aqueous layer extracted with DCM (100 mL). The combined organic extracts were passed through a hydrophobic frit and concentrated under vacuum to leave a solid. Trituration with diethyl ether/pentane (1 :4, 150 mL), and drying under vacuum at 50°C, gave Intermediate 3b (31.7 g, 70%, -90% purity). LCMS: Rt 2.58 min, m/z
289 [MH ]. Ή NMR (300 MHz, CDC13): 1.29 (6H, d, J = 7.0 Hz), 1.45-1.89 (6H, m), 4.26 (2H, apparent quin, J = 6.5 Hz), 6.53 (1H, s), 6.65 (1H, br s), 6.77 (1H, dd, J = 9.0, 3.6 Hz), 7.29-7.28 (1H, ddd, J = 9.0, 8.0, 3.0), 8.02 (1H, d, J = 2.9 Hz).
c. 3-((2S,6R)-2,6-Dimethyl-piperidine-l-yl)-6-fluoro-
[l,2,4]triazolo[4,3-a]pyridine (Intermediate 3c)
Figure imgf000091_0001
To a suspension of Intermediate 3b (27.4 g, 102.9 mmol) and pyridine (25 niL, 309.1 mmol) in toluene (250 mL) at 50°C was added POCl3 (1 1.0 mL, 1 18 mmol) in 3 portions at 30 s intervals. (CARE: exotherm to 70°C) The brown suspension was stirred at 50°C for 1 h, then cooled to RT. Water (100 mL) and sat. aqueous NaHC03 solution (100 mL) were added (CARE: gas evolution) and the mixture stirred at RT for 30 min. The aqueous was extracted with EtOAc (2 x 250 mL), then the combined organics washed with brine (250 mL), dried (Na2S04), filtered and concentrated in vacuo to leave a brown oil (26.3 g, overweight). The oil was redissolved in MeOH (150 mL) then charcoal (6 g) was added and the mixture swirled for 30 min. The suspension was filtered through Celite and the filtercake washed with MeOH (25 mL). The filtrate was concentrated in vacuo to leave a red oil. this was azeotroped with pentane (25 mL) (24.0 g). The solid was slurried in diethyl ether/pentane (1 : 1 , 40 mL), filtered and dried in vacuo to leave Intermediate 3c (20.6 g, 81%). The mother liquor was concentrated in vacuo, the residue dissolved in hot cyclohexane (50°C, 30 mL), then cooled to RT and allowed to stand for over the weekend. The mixture was filtered, the solid washed with cyclohexane (5 mL) then dried in vacuo at 45 °C to leave additional Intermediate 3c (1.8 g, 6%).
LCMS: Rt 3.30 min, m/z 249 [MH ]. Ή NMR (300 MHz, CDC13): 0.68 (6H, d, J = 6.2 Hz), 1.36-1.49 (2H, m), 1.52-1.68 (1H, m), 1.75-1.90 (3H, m), 3.29-3.40 (2H, m), 7.16 (1H, ddd, J = 10.0, 7.6, 2.3 Hz), 7.67 (1H, dd, J = 10.0, 4.7 Hz), 8.03 (1H, t, J = 2.7 Hz). d. (lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-ylamine (Intermediate 3)
To a solution of Intermediate 1 (6.59 g, 40.4 mmol) in dry DMF (80 mL) under N2 was added sodium hydride (60% dispersion in oil, 3.20 g, 80.0 mmol) and the resulting opaque brown solution was stirred at RT for 45 min (CARE: gas evolution). A solution of Intermediate 3c (9.93 g, 40.0 mmol) in dry DMF (20 mL) was added and the solution stirred at RT for 24 h. The reaction was carefully quenched with saturated NH4C1 (CARE: gas evolution) solution and H20. The brown mixture was stirred for 30 min. The mixture was concentrated in vacuo and dissolved in MeOH (125 mL), charcoal was added to the solution and the mixture was stirred at RT for 1 h, and then filtered through Celite. The solution was evaporated under reduced pressure to afford a residue, which was suspended in H20 (100 mL) and extracted with EtOAc (3 X 100 mL). The combined organic extracts were washed with brine (75 mL), dried over Na2S04, filtered and concentrated in vacuo to leave a brown foam (14.6 g, 93%). The foam was triturated with pentane (2 X 75 mL) using sonication and stirring, the solution was decanted and the solid was left to dry under vacuum and at RT affording a solid (14.2 g, 90%). LCMS (Method 3): Rt 2.32 min, m/z 392 [MH+].
Intermediate A
Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-isopropyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester
Figure imgf000092_0001
a. 2-(4-Iodo-pyrazol-l-yl)-ethanol (Intermediate Aa)
Figure imgf000093_0001
H
A solution of 4-iodopyrazole (14.3 g, 73.9 mmol) and ethylene carbonate (6.83g, 77.6 mmol) in DMF (50 mL) was stirred at 125°C for 24 h. The cooled solution was concentrated under vacuum to leave a brown oil. The residue was purified by FCC using 30-70% EtOAc in DCM to give the title compound (9.36 g, 53%). LCMS (Method 3): Rt 2.24 min, m/z 239 [MH+].
b. 2-(5-Amino-3-isopropyl-[l,4']bipyrazolyl-l'-yl)-ethanol
(Intermediate Ab)
Figure imgf000093_0002
A mixture of 5-isopropyl-2H-pyrazol-3-ylamine (550 mg, 4.4 mmol), Intermediate Aa (1.0 g, 4.2 mmol), copper (I) iodide (40 mg, 0.21 mmol), (1 S,2S)- N.N'-dimethyl cyclohexane-l ,2-diamine (1 19 mg, 0.84 mmol) and potassium carbonate (1.22 g, 8.8 mmol) in Xylene (5 mL) was de-gassed and flushed with argon (3x). The reaction mixture was heated at 1 10°C for 18 h. The mixture was poured onto a SCX-2 and eluted with MeOH and 2M NH3 in MeOH. The basic fractions were evaporated under reduced pressure to afford a brown gum. The residue obtained was purified by FCC, using 0-10% MeOH in DCM to afford the title compound (0.25 g, 25%). LCMS (Method 3): Rt 1.43 min, m/z 236 [MH+].
c. [l,-(2-Hydroxy-ethyl)-3-isopropyl-l,H-[l,4,]bipyrazolyl-5-yl]- carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate Ac)
Figure imgf000094_0001
A solution of Intermediate Ab (250 mg, 1.06 mmol) in EtOAc (3 mL) was treated with aqueous NaOH (1M, 1.9 mmol), followed by 2,2,2-trichloroethyl chloroformate (154 μΕ, 1.16 mmol) and the reaction mixture was stirred at RT for 1 h. The mixture was partitioned between EtOAc (10 mL) and water (10 mL). The layers were separated and the aqueous layer was extracted with a further 10 mL EtOAc. The combined organic layers were dried (Na2S04), filtered and concentrated in vacuo. The residue was purified by FCC, using 0-40% EtOAc in DCM to afford the title compound as a colourless oil (300 mg, 70%>). LCMS (Method 3): Rt 3.42 min, m/z 410, 412 [MH+].
d. l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[l'- (2-hydroxy-ethyl)-3-isopropyl-l'H-[l,4']bipyrazolyl-5-yl]-urea. (Intermediate Ad)
Figure imgf000094_0002
A mixture of Intermediate 3 (286 mg, 0.730 mmol), Intermediate Ac (300 mg, 0.730 mmol) and DIPEA (183 μί, 1.09 mmol) in dioxane (3 mL) was stirred at 70 °C O/N. The reaction mixture was poured on a SCX-2 cartridge eluting with MeOH and 2M NH3 in MeOH. The basic fractions were concentrated in vacuo and the resultant residue was purified by FCC eluting with 0-100%) MeOH in DCM to afford the title compound (375 mg, 80%). LCMS (Method 3): Rt 3.47 min, m/z 653 [MH+]. e. Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-isopropyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester (Intermediate A)
Figure imgf000095_0001
A mixture of Intermediate Ad (375 mg, 0.575 mmol), methanesulfonyl chloride (182 μΐ,, 1.72 mmol) and DIPEA (383 μΐ,, 2.3 mmol) in DCM (5 mL) was stirred at RT for 60 min. The reaction mixture was partitioned between DCM and water. The organic layer was washed with brine, separated through a phase separating cartridge and concentrated in vacuo to afford the title compound (400 mg, 95%). LCMS (Method 3): Rt 3.68 min, m/z 731 [MH+].
Intermediate B
Methanesulfonic acid 2-[3-isopropyl-5-(3-{(lS,4R)-4-[3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-[l,4']bipyrazolyl-l '-yl]-ethyl ester
Figure imgf000095_0002
a. l-{(lS,4R)-4-[3-((S)-2-methyl--piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[l '-(2-hydroxy-ethyl)- 3-isopropyl-l'H-[l,4,]bipyrazolyl-5-yl]-urea. (Intermediate Ba)
Figure imgf000096_0001
The title compound was prepared starting from Intermediate 2 (150 mg, 0.397 mmol), and Intermediate Ac (163 mg, 0.397 mmol) by using an analogous procedure to that described for Intermediate A step d. LCMS (Method 3): Rt 3.21 min, m/z = 639 [MH+].
Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}- ureido)-3-isopropyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester (Intermediate B)
Figure imgf000096_0002
The title compound was prepared starting from Intermediate Ba (217 mg, 0.340 mmol) by using an analogous procedure to that described for Intermediate A step d. LCMS (Method 3): Rt 3.40 min, m/z = 717 [MH+].
Intermediate C
Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-ethyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester
Figure imgf000097_0001
2-(5-Amino-3-ethyl-[l,4']bipyrazolyl-l'-yl)-ethanol (Intermediate
Figure imgf000097_0002
The title compound was prepared starting from Intermediate Aa (1 g, 8.99 mmol) by using an analogous procedure to that described for Intermediate A step b. LCMS (Method 3): Rt 0.41 min, m/z = 222 [MH+].
b. [l'-il-Hydroxy-ethy -S-ethyyl-l'H-Il^'Jbipyrazolyl-S-yl]- carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate Cb)
Figure imgf000097_0003
The title compound was prepared starting from Intermediate Ca (653 mg, 2.95 mmol) by using an analogous procedure to that described for Intermediate A step c. LCMS (Method 3): Rt 3.22 min, m/z = 396, 398 [MH+].
c. l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[l'- (2-hydroxy-ethyl)-3-ethyl-l'H-[l,4,]bipyrazolyl-5-yl]-urea. (Intermediate Cc)
Figure imgf000098_0001
The title compound was prepared starting from Intermediate 3 (900 mg, 2.27 mmol), and Intermediate Cb (889 mg, 2.27 mmol) by using an analogous procedure to that described for Intermediate A step d. LCMS (Method 3): Rt 3.30 min, m/z = 639 [MH+].
d. Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-ethyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester (Intermediate C)
Figure imgf000098_0002
The title compound was prepared starting from Intermediate Cc (200 mg, 0.31 mmol) by using an analogous procedure to that described for Intermediate A step e. LCMS (Method 3): Rt 3.48 min, m/z = 717 [MH+].
Intermediate D
Methanesulfonic acid 2-[3-ethyl-5-(3-{(lS,4R)-4-[3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-[l,4']bipyrazolyl-l '-yl]-ethyl ester
Figure imgf000099_0001
a. l-{(lS,4R)-4-[3-((S)-2-methyl--piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[l '-(2-hydroxy-ethyl)- 3-ethyl-l'H-[l,4']bipyrazolyl-5-yl]-urea. (Intermediate Da)
Figure imgf000099_0002
The title compound was prepared starting from Intermediate 2 (150 mg, 0.397 mmol), and Intermediate Cb (157 mg, 0.397 mmol) by using an analogous procedure to that described for Intermediate A step d. LCMS (Method 3): Rt 3.10 min, m/z = 625 [MH+].
b. Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-ethyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester (Intermediate D)
Figure imgf000099_0003
The title compound was prepared starting from Intermediate Da (140 mg, 0.224 mmol) by using an analogous procedure to that described for Intermediate A step e. LCMS (Method 3): Rt 3.27 min, m/z = 703 [MH+]. Intermediate E
Methanesulfonic acid 2-{3-[3-methyl-5-(3-{(lS,4R)-4-[3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-pyrazol-l-yl]-phenoxy}-ethyl ester
Figure imgf000100_0001
a. 3-(5-Amino-3-methyl-pyrazol-l-yl)-phenol (Intermediate Ea)
Figure imgf000100_0002
The title compound was prepared starting from 5-methyl-2H-amino pyrazol (500 mg, 5.1 mmol) and 3-iodo-phenol (1.25 g, 5.7 mmol) by using an analogous procedure to that described for Intermediate A step b. LCMS (Method 3): Rt 0.99 min, m/z = 190 [MH+].
b. 5-methyl-2-{3-[2-(tetrahydro-pyran-2-yloxy)-ethoxy]-phenyl}-2H- pyrazol-3-ylamine (Intermediate
Figure imgf000100_0003
DIAD (629 μΐ,, 3.98 mmol) was added slowly to a solution of Intermediate Ea (378 mg, 1.99 mmol), 2-(tetrahydro-pyran-2-yloxy)-ethanol (270 μί, 2.98 mmol) and triphenylphosphine (1.04 g, 3.98 mmol) in THF (15.0 mL) and stirred for 1 h. The reaction mixture was poured on a SCX-2 cartridge eluting with MeOH and 2M NH3 in MeOH. The basic fractions were concentrated in vacuo and the resultant residue was purified by FCC eluting with 0-60% EtOAc in DCM to afford the title compound (280 mg, 60%) as a pale yellow gum. Ή NMR (300 MHz, CDC13): 2.05 (3H, s), 3.71 (2H, q, J = 5.09 Hz), 4.01 (2H, t, J = 5.09 Hz), 4.86 (1H, t, J = 5.875 Hz), 5.23 (2H, br s), 5.30 (1H, s), 6.79-685 (1H, m), 7.08-7.15 (2H, m), 7.27-7.36 (lH, m).
c. (5-tert-Butyl-2-{3-[2-(tetrahydro-pyran-2-yloxy)-ethoxy]-phenyl}- 2H-pyrazol-3-yl)-carbamic acid ethyl ester (Intermediate Ec)
Figure imgf000101_0001
The title compound was prepared starting from 2,2,2- trichloroethylchloroformate and Intermediate Eb by using an analogous procedure to that described for Example A step c. LCMS (Method 3): Rt 3.41 , m/z 408, 410 [MH+].
d. l-{2-[3-(2-Hydroxy-ethoxy)-phenyl]-5-methyl-2H-pyrazol-3-yl}-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate Ed)
Figure imgf000101_0002
The title compound was prepared starting from Intermediate Ec and Intermediate 2 by using an analogous procedure to that described for Intermediate A step d. LCMS (Method 3): Rt 3.22 min, m/z = 637 [MH+].
e. Methanesulfonic acid 2-{3-[3-methyl-5-(3-{(lS,4R)-4-[3-((S)-2- methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-pyrazol-l-yl]-phenoxy}-ethyl ester (Intermediate E)
Figure imgf000102_0001
The title compound was prepared starting from Intermediate 4b and Intermediate l g by using an analogous procedure to that described for Intermediate A step e. LCMS (Method 3): Rt 3.98 min, m/z = 715 [MH+].
Intermediate F
Methanesulfonic acid 2-[5-(3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-isopropyl-[l,4']bipyrazolyl-l'-yl]-ethyl ester
Figure imgf000102_0002
a. 2-(3-Iodo-pyrazol-l-yl)-ethanol (Intermediate Fa)
OH
A solution of 3-iodo-lH-pyrazole (500 mg, 2.6 mmol) and ethylene carbonate (238 mg, 2.70 mmol) was formed in DMF (5 mL) and heated at 150°C for 3 h. The mixture was allowed to cool then evaporated under vacuum to remove the solvent. Purification of the residue by FCC eluting with a gradient of 0-100% EtOAc in cyclohexane gave crude title compound (444 mg, 72%) as a brown oil. LCMS (Method 3): Rt 2.17 min, m/z 239 [MH+].
b. 2-(5-Amino-3-isopropyl-[l,3']bipyrazolyl-l'-yl)-ethanol (Intermediate Fb)
Figure imgf000103_0001
A mixture of 5-isopropyl-2H-pyrazol-3-ylamine (376 mg, 3.0 mmol), Intermediate Fa (650 mg, 2.73 mmol), copper (I) iodide (52 mg, 0.273 mmol), (l S,2S)-N.N'-dimethyl cyclohexane-l ,2-diamine (78 mg, 0.546 mmol) and potassium carbonate (792 mg, 5.73 mmol) in Toluene (6 mL) was de-gassed and flushed with argon (3x). The reaction mixture was microwaved at 150°C for 3 h. The mixture was poured onto a SCX-2 and eluted with MeOH and 2M NH3 in MeOH. The basic fractions were evaporated under reduced pressure to afford a brown gum. The residue obtained was purified by FCC, using 0-10% MeOH in DCM, to afford the title compound (0.28 g, 30%). LCMS (Method 3): Rt 1.92 min, m/z 236 [MH+].
c. [l,-(2-Hydroxy-ethyl)-3-isopropyl-l,H-[l,3,]bipyrazolyl-5-yl]- carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate Fc)
Figure imgf000103_0002
A solution of Intermediate Fb (272 mg, 1.16 mmol) in EtOAc (3 mL) was treated with aqueous NaOH (1M, 2.10 mmol), followed by 2,2,2-trichloroethyl chloroformate (167 μί, 1.21 mmol) and the reaction mixture was stirred at RT for 1 h. The mixture was partitioned between EtOAc (10 mL) and water (10 mL). The layers were separated and the aqueous layer was extracted with a further 10 mL EtOAc. The combined organic layers were dried (Na2S04), filtered and concentrated in vacuo. The residue was purified by FCC, using 10-100%) EtOAc in DCM, to afford the title compound as a colourless oil (124 mg, 40%>). LCMS (Method 3): Rt 3.98 min, m/z 410, 412 [MH+] .
d. l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[l '- (2-hydr oxy-ethyl)-3-isopropyl- 1 Ή- [ 1 ,3 '] bipyrazolyl-5-yl] -urea. (Intermediate Fd)
Figure imgf000104_0001
A mixture of Intermediate 3 (1 19 mg, 0.303 mmol), Intermediate Fc (124.5 mg, 0.303 mmol) and DIPEA (76 μί, 0.454 mmol) in dioxane (2 mL) was stirred at 60 °C overnight. The reaction mixture was poured on a SCX-2 cartridge eluting with MeOH and 2M N¾ in MeOH. The basic fractions were concentrated in vacuo and the resultant residue was purified by FCC, eluting with 0-20% MeOH in DCM, to afford the title compound (100 mg, 60%). LCMS (Method 3): Rt 3.70 min, m/z 653.4 [MH+] .
Methanesulfonic acid 2-[5-(3-{(l S,4R)-4-[3-((2S,6R)-2,6-dimethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-3-isopropyl-[l,3']bipyrazolyl-l '-yl]-ethyl ester (Intermediate F)
Figure imgf000104_0002
A mixture of Intermediate Fd (100 mg, 0.153 mmol), methanesulfonyl chloride (32 \iL, 0.46 mmol) and DIPEA (103 μΕ, 0.61 mmol) in DCM (5 mL) was stirred at RT for 60 min. The reaction mixture was partitioned between DCM and water. The organic layer was washed with brine, separated through a phase separating cartridge and concentrated in vacuo to afford the title compound (1 12 mg, quantitative). LCMS (Method 3): Rt 3.98 min, m/z 731 [MH+].
Intermediate G
Methanesulfonic acid 2-[3-tert-butyl-5-(3-{(lS,4R)-4-[3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-[l,4']bipyrazolyl-l '-yl]-ethyl ester.
Figure imgf000105_0001
2-(4-Iodo-pyrazol-l-yl)-ethanol (Intermediate Ga)
Figure imgf000105_0002
H
A solution of 4-iodopyrazole (14.3 g, 73.9 mmol) and ethylene carbonate (6.83 g, 77.6 mmol) in DMF (50 mL) was stirred at 125 °C for 24 h. The cooled solution was concentrated under vacuum to leave a brown oil. The residue was purified by FCC, using 30-70% EtOAc in DCM, to give the title compound (9.36 g, 53%). LCMS (Method 1): Rt 2.24 min, m/z 239 [MH+].
b. 2-(5-Amino-3-tert-butyl-[l,4']bipyrazolyl-l'-yl)-ethanol
(Intermediate Gb)
Figure imgf000105_0003
A solution of Intermediate Ga (9.36 g, 39.3 mmol) in xylene (40 mL) was purged with argon for 30 min. In a separate flask, a mixture of 3-tert-butyl-lH- pyrazole-5-amine (5.75 g, 41.3 mmol), copper iodide (375 mg, 1.97 mmol), trans- N,N-dimethylcyclohexane-l ,2-diamine (1.12 g, 7.87 mmol) and potassium carbonate (1 1.4 g, 82.6 mmol) was de-gassed and purged with argon three times. The xylene solution was then added, via cannula to the flask and the resultant brown solution was heated at reflux for 3 h. The cooled solution was diluted with EtOAc (40 mL) and washed with saturated aqueous ammonia solution/water (1 : 1 , 40 mL). The aqueous layer was extracted with EtOAc (40 mL) and the combined organics were washed with water (40 mL) and brine (40 mL), dried (Na2S04), filtered and concentrated in vacuo to afford a solid. This was purified by FCC, using 4-7.5% MeOH in DCM to afford the title compound (6.01 g, 61 %). LCMS (Method 1): Rt 1.84 min, m/z 250 [MH+].
c. [3-tert-Butyl-l'-(2-hydroxy-ethyl)-l'H-[l,4']bipyrazolyl-5-yl]- carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate Gc)
Figure imgf000106_0001
To a solution of Intermediate Gb (6.01 g, 24.1 mmol) and aqueous NaOH solution (36 mL, 36 mmol) in EtOAc (40 mL) was added 2,2,2-trichloroethyl chloroformate (4.15 mL, 30.1 mmol). The reaction mixture was stirred at RT for 16 h. The layers were separated and the aqueous layer was extracted with EtOAc (40 mL). The combined organics were washed with brine (40 mL), dried (Na2S04), filtered and concentrated in vacuo to afford a brown gum. This was purified by FCC, using 2-7% MeOH in DCM to afford the title compound. LCMS (Method 1): Rt 3.61 min, m/z 424, 426 [MH+]. d. l-[3-tert-Butyl-l'-(2-hydroxyethyl)l 'H[l,4']bipyrazolyl-5-yl]-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea. (Intermediate Gd)
Figure imgf000107_0001
A mixture of Intermediate Gc (100 mg, 235 μιηοΐ), Intermediate 2 (89.0 mg, 235 μιηοΐ) and DIPEA (61.0 μί, 353 μιηοΐ) in dioxane (1.5 mL) was heated at 60°C for 48 h. The mixture was cooled to RT, diluted with DCM (5 mL) and washed with water (5 mL) and brine (5 mL). The organic layer was passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to afford the title compound (77 mg, 50%).. LCMS (Method 1): Rt 3.32 min, m/z 653 [MH+].
e. Methanesulfonic acid 2-[3-tert-butyl-5-(3-{(lS,4R)-4-[3-((S)-2- methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-[l,4']bipyrazolyl-l '-yl]-ethyl ester. (Intermediate G)
A mixture of Intermediate Gd (75.0 mg, 1 15 μιηοΐ), methanesulfonyl chloride (1 1.6 μί,149 μιηοΐ) and DIPEA (60.0 iL, 345 μιηοΐ) in DCM (1 mL) was stirred at RT for 30 min. The reaction mixture was partitioned between DCM (5 mL) and water (2 x 5 mL). The organic layer was washed with brine (5 mL), separated through a phase separating cartridge and concentrated in vacuo to afford the title compound (84 mg, 100%). LCMS (Method 1): Rt 3.54 min, m/z 731 [MH+].
Intermediate H
Methanesulfonic acid 3- [3-tert-butyl-5-(3-{(lS,4R)-4- [3-((S)-2-methyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-pyrazol-l-yl]-benzyl ester
Figure imgf000108_0001
a. 5-tert-Butyl-2-(3-hydroxymethyl-phenyl)-2H-pyr azol-3-yl] - carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate Ha)
Figure imgf000108_0002
To a bi-phasic mixture of [3-(5-Amino-3-tert-butyl-pyrazol-l-yl)-phenyl]- methanol (US. 20080248548; 737 mg, 3.00 mmol) in EtOAc (22.5 mL) and IN NaOH solution (8.1 1 mL, 8.1 1 mmol) at 0°C was added 2,2,2-trichloroethyl chloroformate (0.45 mL, 3.30 mmol) and the mixture stirred for 1.25 h. The layers were separated and the organic layer was washed with brine, dried and concentrated in vacuo to give the title compound (1.26 g, 99%). LCMS (Method 1): Rt 4.00 min, m/z 420, 422 [MH+].
b. l-[5-tert-Butyl-2-(3-hydroxymethyl-phenyl)-2H-pyrazol-3-yl]-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate Hb)
Figure imgf000108_0003
A mixture of Intermediate Ha (319 mg, 0.85 mmol), Intermediate 2 (335 mg, 0.85 mmol) and DIPEA (294 μί, 1.69 mmol) in dioxane (10 mL) was stirred at 80°C for 18 h. After cooling, the reaction mixture was partitioned between water and DCM. The aqueous phase was extracted with EtOAc (x 3) and the combined organic layers were washed with brine, dried (MgS04) and concentrated in vacuo. The resulting residue was purified by FCC on silica, using a gradient of 0-10% MeOH in DCM, to afford the title compound (362 mg, 66%). LCMS (Method 2): Rt 3.29 min, m/z 649 [MH+].
c. Methanesulfonic acid 3-[3-tert-butyl-5-(3-{(lS,4R)-4-[3-((S)-2- methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-ureido)-pyrazol-l-yl]-benzyl ester (Intermediate H)
To an ice-bath cooled solution of Intermediate Hb (316 mg, 0.49 mmol) in DCM (5.0 mL) was added DIPEA (339 iL, 1.95 mmol) followed by methanesulfonyl chloride (76 μί, 0.97 mmol). The reaction mixture was stirred for 3 h and then quenched with water. The aqueous phase was extracted with DCM (x 3) and the combined organic layers were washed with brine, dried (MgS04) and concentrated in vacuo to afford the title compound (Quantitative, assumed 0.49 mmol). The isolated product was used in the following step without further purification. LCMS (Method 2): Rt 3.61 min, m/z 727 [MH+].
Examples 1-12
General displacement procedure
A mixture of Intermediate A-H (0.14 mmol) and an appropriate amine [see table 2] (2.15 mmol) in anhydrous THF (3 mL) was stirred at 40 °C for 18 h in a sealed vial. The volatiles were concentrated in vacuo and the resultant residue was purified by HPLC (Gemini CI 8, 20-40% MeCN in H20, 0.1% HC02H, 18ml/min.) and freeze dried to afford the title compound (30-50%>).
Figure imgf000110_0001
(continued)
Figure imgf000111_0001
(continued)
Figure imgf000112_0001
(continued)
Figure imgf000113_0001
(continued)
Figure imgf000114_0001
(continued)
Figure imgf000115_0001
(continued)
Ή NMR (400 MHz, d6-DMSO): 0.86
(3H, d, J = 6.2 Hz), 1.23 (9H, s), 1.42- 1.50 (2H, m), 1.58-1.67 (2H, m), 1.72- 1.84 (3H, m), 1.84-1.91 (3H, m), 1.91
(6H, s), 1.95-2.10 (2H, m), 2.66-2.77
(3H, m), 2.86 (1H, ddd, J = 12.7, 9.0, (Method 3.9 Hz), 3.08-3.16 (1H, dt, J = 12.3, 3): Rt: 4.3 Hz), (m, obscured by solvent 3.34
Figure imgf000116_0001
peak), 3.56 (2H, s), 4.77 (1H, td, J = min, l- {5-tert-Butyl-2-[3-(3-dimethylamino-azetidin-l- 8.4, 5.7 Hz), 5.47 (1H, t, J = 4.2 Hz), m/z 731 ylmethyl)-phenyl]-2H-pyrazol-3-yl} -3- {(l S,4R)-4- 6.28 (1H, s), 7.02 (1H, d, J = 8.6 Hz), [MH+] [3-((S)-2-methyl-piperidin- 1 -yl)- 7.14 (1H, dd, J = 9.8, 2.1 Hz), 7.20- [l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l ,2,3,4- 7.25 (3H, m), 7.25-7.34 (4H, m), 7.38 tetrahydro-naphthalen-l-yl}-urea formate salt (1H, t, J = 7.7 Hz), 7.59 (1H, d, J =
10.0 Hz), 7.64 (1H, d, J = 1.8 Hz), 8.06
(1H, s), 8.25 (0.5H, s).
Example 13
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((lS,4R)-4-{3-[2,6-dichloro- 4-(4-methyl-piperazin- l-ylmethyl)-phenyl] -[1,2,4] triazolo [4,3-a] pyridin-6- yloxy}-l,2,3,4-tetrahydro-naphthalen-l-yl)-urea formate salt
Figure imgf000117_0001
a. {3,5-Dichloro-4-[(5-fluoro-pyridin-2-yl)-hydrazonomethyl]- iyl}-methanol (Intermediate 13a)
Figure imgf000117_0002
A solution of 2,6-dichloro-4-hydroxymethylbenzaldehyde (0.50 g, 2.40 mmol) in propionitrile (4.8 mL) was treated with (5-fluoro-pyridin-2-yl)-hydrazine (0.41 g, 2.40 mmol) and the solution was heated to 85°C for 2 h then cooled to 0°C. The solid was filtered off, washed with ice-cold propionitrile and dried at 50°C in vacuo to give the title compound (0.73 g, 95%). LCMS (Method 3): Rt 3.55 min, m/z 314, 316 [MH+].
b. [3,5-Dichloro-4-(6-fluoro-[l,2,4]triazolo[4,3-a]pyridin-3-yl)- phenylj-methanol (Intermediate 13b)
Figure imgf000117_0003
A suspension of Intermediate 13a (0.73 g, 2.30 mmol) in DCM (7.3 mL) was treated with diacetoxyiodobenzene (0.79 g, 2.40 mmol) and the solution was cooled to 0°C. MeOH (0.8 mL) was added dropwise and the mixture was stirred at ambient temperature for 4.5 h. The mixture was cooled to 0°C and treated slowly with aqueous IN NaOH solution (~ 5 mL). The layers were separated and the aqueous layer was then extracted with DCM (2 x). The combined organic layers were washed with water and brine, dried (Na2S04), filtered and evaporated in vacuo. The aqueous layers were combined and extracted with 20% MeOH in DCM. The organic layer was dried (Na2S04), filtered and evaporated in vacuo. The residues were dissolved in MeOH/DCM, combined, preadsorbed onto diatomaceous earth and purified by FCC, using 0-8% MeOH in DCM, to give the title compound (0.57 g, 78%). LCMS (Method 3): Rt 2.72 min, m/z 312, 314 [MH+].
c. 3,5-Dichloro-4-(6-fluoro-[l,2,4]triazolo[4,3-a]pyridin-3-yl)- benzaldehyde (Intermediate 13c)
Figure imgf000118_0001
A suspension of Intermediate 13b (0.57 g, 1.80 mmol) in DCM (18 mL) at
0°C was treated with Dess Martin periodinane (0.85 g, 2.00 mmol) and the solution was stirred at 0°C for 1 h. The mixture was diluted with a saturated aqueous sodium bicarbonate solution and the layers were separated. The aqueous layer was then extracted with DCM (2 x). The combined organic layers were washed with a saturated aqueous sodium bicarbonate solution and brine, dried (Na2S04), filtered and evaporated in vacuo. The residue was dissolved in DCM containing a few drops of MeOH and purified by FCC, using 0-5% MeOH in DCM, to give the title compound (0.66 g, quant). LCMS (Method 3): Rt 3.03 min, m/z 328, 330 [MH+]. d. 3-[2,6-Dichloro-4-(4-methyl-piperazin-l-ylmethyl)-ph
fluoro-[l,2,4]triazolo[4,3-a]pyridine (Intermediate 13d)
Figure imgf000119_0001
A cloudy solution of Intermediate 13c (0.66 g, 1.80 mmol) in DCE (18 mL) was treated with 1-methylpiperazine (0.22 mL, 2.00 mmol) then sodium triacetoxyborohydride (0.58 g, 2.70 mmol), and the solution was stirred at ambient temperature overnight. The mixture was treated with another portion of 1- methylpiperazine (0.22 mL, 2.00 mmol) and sodium triacetoxyborohydride (0.58 g, 2.70 mmol), and stirred for 5 h. The mixture was cautiously diluted with a saturated aqueous sodium bicarbonate solution and the layers were separated. The aqueous layer was then extracted with DCM (2 x). The combined organic layers were washed with a saturated aqueous sodium bicarbonate solution and brine, dried (Na2S04), filtered and evaporated in vacuo. The residue was dissolved in DCM and purified by FCC, using 0-8% [2M NH3 in MeOH] in DCM, to give the title compound (0.41 g, 57%). LCMS (Method 3): Rt 2.1 1 min, m/z 394, 396 [MH+].
e. (lS,4R)-4-{3-[2,6-Dichloro-4-(4-methyl-piperazin-l-ylmethyl)- phenyl]-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy}-l,2,3,4-tetrahydro-naphthalen-l- ylamine (Intermediate 13e
Figure imgf000119_0002
A suspension of sodium hydride (60%> dispersion in oil, 56 mg, 1.39 mmol) in dry DMF (2.0 mL) under nitrogen was treated with Intermediate 1 (46 mg, 0.28 mmol) and the mixture was stirred for 20 min. Intermediate 13d (1 10 mg, 0.28 mmol) was added and the mixture was heated to 60°C for 1.75 h then quenched with MeOH. The mixture was further diluted with MeOH and applied to a preconditioned (MeOH) SCX-2 cartridge (10g). The cartridge was eluted with MeOH then 2M NH3 in MeOH. The relevant fractions were combined and concentrated in vacuo. The residue was dissolved in DCM containing a few drops of MeOH and purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to give the title compound (28 mg, 19%). LCMS (Method 3): Rt 2.08 min, m/z 537, 539 [MH+].
f. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((lS,4R)-4-{3-[2,6- dichloro-4-(4-methyl-piperazin- l-ylmethyl)-phenyl] - [1,2,4] triazolo [4,3- a]pyridin-6-yloxy}-l,2,3,4-tetrahydro-naphthalen-l-yl)-urea formate salt (Example 13)
(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-carbamic acid 2,2,2-trichloro-ethyl ester (US 20040192653; 17 mg, 0.051 mmol) was treated with a solution of Intermediate 13e (25 mg, 0.047 mmol) in 1 ,4-dioxane (0.4 mL) then DIPEA (10 μΐ,, 0.058 mmol) was added. The mixture was stirred at 70°C overnight. The mixture was treated with another portion of (5-tert-butyl-2-methyl-2H-pyrazol-3-yl)- carbamic acid 2,2,2-trichloro-ethyl ester (7.8 mg, 0.024 mmol) in 1 ,4-dioxane (0.1 mL) and stirred at 70°C for 6 h. The mixture was evaporated in vacuo and the residue was partitioned between DCM and water. The aqueous layer was then extracted with DCM (2 x). The combined organic layers were washed with brine, dried (Na2S04), filtered and evaporated in vacuo. The residue was purified by HPLC in 4 portions (Method 6, 5-95% MeCN in H20, 0.1% HC02H over 30 min), and the isolated product triturated (diethyl ether) to give a solid. The solid was collected by filtration, washed with ether and dried at 50°C in vacuo to give the title compound (5.4 mg, 16%). LCMS (Method 5): Rt 3.27 min, m/z 716.3 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.20 (9H, s), 1.82-1.99 (2H, m), 1.99-2.05 (1H, m), 2.05-2.14 (1H, m), 2.18 (3H, s), 2.29-2.42 (4H, br s), 2.42-2.52 (4H, br s, partially obscured by DMSO), 3.56 (3H, s, partially obscured by water), 3.60 (2H, s, partially obscured by water), 4.79-4.88 (1H, m), 5.54 (1H, t, J = 4.3 Hz), 6.01 (1H, s), 6.91 (1H, d, J = 8.4 Hz), 7.23-7.30 (1H, m), 7.33-7.39 (4H, m), 7.66 (2H, s), 7.91 (1H, d, J = 10.2 Hz), 7.97 (1H, d, J = 1.7 Hz), 8.21 (1H, s), 8.29 (1H, s).
Example 14
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt
Figure imgf000121_0001
a. 6-Fluoro-[l,2,4]triazo ridine (Intermediate 14a)
Figure imgf000121_0002
(5-Fluoro-pyridin-2-yl)-hydrazine (500 mg, 3.93 mmol) in diethoxymethyl acetate (5 mL) was stirred at RT for 2 h. The resulting precipitate was diluted with cyclohexane (5 mL) and filtered to give the title compound (379 mg, 70%). Ή NMR
(400 MHz, CDC13): 7.25 (1H, m), 7.84 (1H, m), 8.09 (1H, t), 8.84 (1H, s).
b. 3-Chloro-6-fluoro-[l,2,4]triazolo[4,3-a]pyridine (Intermediate 14b)
Figure imgf000121_0003
A solution of Intermediate 14a (789 mg, 5.98 mmol) and N- chlorosuccinimide (878 mg, 6.57 mmol) in chloroform (15 mL) was heated at 65°C overnight. The cooled mixture was washed with sat. aq. NaHC03 solution (2 x 15 mL) and dried (Na2S04). The solvent was evaporated, then the residue suspended in diethyl ether (10 mL) and filtered to give the title compound (730 mg, 76%). LCMS (Method 1): Rt 1.83 min, m/z 172 [MH+]. c. [l-(6-Fluoro-[l,2,4]triazolo[4,3-a]pyridin-3-yl)-piperidin
methanol (Intermediate 14c)
Figure imgf000122_0001
A solution of Intermediate 14b (593 mg, 3.45 mmol) and 4- piperidinemethanol (1.59 g, 13.8 mmol) in NMP (10 mL) was heated in the microwave at 170°C for 3 h. The cooled mixture was applied to an SCX-2 cartridge (70 g), washing with methanol then eluting basic components with 0.4 - 2 M NH3 in MeOH. Product containing fractions were combined and concentrated in vacuo. The residue was purified by FCC, using 0- 15% MeOH in EtOAc, to give the title compound as a brown gum (481 mg, 56%). LCMS (Method 3): Rt 2.12 min, m/z 251 [MH+] .
d. 6-Fluoro-3-(4-triisopropylsilanyloxymethyl-piperidin- 1-yl)- [l,2,4]triazolo[4,3-a]pyridin (Intermediate 14d)
Figure imgf000122_0002
solution of Intermediate 14c (470 mg, 1.88 mmol) and Et3N (390 \iL,
2.82 mmol) in DCM (5 mL) was added triisopropylsilyltrifluoromethane sulfonate (607 μί, 2.26 mmol) and the mixture stirred at RT for 0.5 h. The mixture was washed with sat. aq. NaHC03 solution, dried and concentrated in vacuo. The residue was purified by FCC, using 0- 100%) EtOAc in cyclohexane, then 10%> MeOH in EtOAc, to give the title compound (565 mg, 74%). LCMS (Method 3): Rt 5.21 min, m/z 407 [MH+] .
e. (1 S,4R)-4- [3-(4-Triisopropylsilanyloxymethyl-piperidin- 1-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-ylamine (Intermediate 14e)
Figure imgf000123_0001
To a solution of Intermediate 1 (223 mg, 1.37 mmol) in DMF (3 mL) was added NaH (60% dispersion in oil, 168 mg, 4.20 mmol) and the mixture stirred at RT for 15 min. A solution of Intermediate 14d (555 mg, 1.37 mmol) in DMF (3 mL) was added and the mixture stirred at 60°C for 1.75 h. The cooled mixture was diluted with water and extracted with DCM (5 x 20 mL). The combined organics were dried and concentrated in vacuo. The residue was purified by FCC, using 0- 14%) [2M NH3 in MeOH] in DCM, to give the title compound as a brown gum (344 mg, 46%). LCMS (Method 3): Rt 3.29 min, m/z 550 [MH+].
f. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- triisopropylsilanyloxymethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 14f)
Figure imgf000123_0002
A solution of (5-tert-butyl-2-methyl-2H-pyrazol-3-yl)-carbamic acid 2,2,2- trichloro-ethyl ester (US 20040192653, 90 mg, 0.273 mmol), Intermediate 14e (150 mg, 0.273 mmol) and DIPEA (71 μί, 0.409 mmol) in dioxane (2 mL) was heated at 60°C for 48 h. The reaction mixture was cooled and diluted with DCM (5 mL). The organic layer was washed with water (3 x 5 mL), passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0- 10% [2M NH3 in MeOH] in DCM, to afford the title compound (1 17 mg, 59%). LCMS (Method 3): Rt: 5.12 min, m/z 729 [MH+].
g. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- hydr oxymethyl-piperidin- 1-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - 1 ,2,3,4- tetrahydro-naphthalen-l-yl}-urea (Intermediate 14g)
Figure imgf000124_0001
Tetrabutylammonium fluoride (1M solution in THF, 21 1 μΐ^, 0.21 1 mmol) was added to a solution of Intermediate 14f (1 10 mg, 0.151 mmol) in THF (3 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (2 x 5 mL) and brine (5 mL). The organic layer was passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to afford the title compound (52 mg, 60%). LCMS (Method 3): Rt: 2.91 min, m/z 573 [MH+].
h. Methanesulfonic acid l-(6-{(lR,4S)-4-[3-(5-tert-butyl-2-methyl- 2H-pyrazol-3-yl)-ureido]-l,2,3,4-tetrahydro-naphthalen-l-yloxy}-
[l,2,4]triazolo[4,3-a]pyridin-3-yl)-piperidin-4-ylmethyl ester (Intermediate 14h)
Figure imgf000124_0002
Methanesulfonyl chloride (8.8 \iL, 0.13 mmol) was added to a solution of Intermediate 14g (50 mg, 0.08 mmol) and DIPEA (46 μΐ,, 0.26 mmol) in DCM (1.5 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (3 x 5 mL). The organic layer was passed through a phase separator and concentrated in vacuo to afford the title compound (57 mg, 100%). LCMS (Method 3): Rt: 3.21 min, m/z 651 [MH+].
i. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt (Example 14)
A solution of Intermediate 14h (57 mg, 0.08 mmol) and dimethylamine (2M in THF, 0.873 mL, 1.75 mmol) in THF (4 mL) was heated at 60°C for 18 h. A further 1.4 mL of dimethylamine (2M in THF) was added and the reaction mixture was heated at 65°C for 48 h. The reaction mixture was cooled to RT and the solvent was removed by air, to leave a residue which was purified by MDAP (Method 7), to afford the title compound (18 mg, 35%). LCMS (Method 5): Rt: 3.00 min, m/z 600.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.20 (9H, s), 1.32-1.47 (2H, m), 1.64-1.76 (1H, m), 1.76-1.86 (2H, m), 1.87-2.12 (4H, m), 2.13-2.21 (9H, m), 2.86-2.99 (2H, m, obscured by water), 3.35-3.48 (2H, m, obscured by water), 3.57 (2H, s, obscured by water), 4.82-4.91 (1H, m), 5.57 (1H, t, J = 4.5 Hz), 6.02 (1H, s), 6.91 (1H, d, J = 8.8 Hz), 7.18 (1H, dd, J = 9.8, 1.9 Hz), 7.26-7.33 (1H, m), 7.34-7.43 (3H, m), 7.62 (1H, d, J = 10.0 Hz), 7.65 (1H, d, J = 1.8 Hz), 8.19 (1H, s), 8.27 (1H, s).
Example 15
l-(5-tert-Butyl-isoxazol-3-yl)-3-{(lS,4R)-4-[3-(4-dimethylaminomethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-urea
Figure imgf000125_0001
a. l-(5-tert-Butyl-isoxazol-3-yl)-3-{(lS,4R)-4-[3-(4- triisopropylsilanyloxymethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 15a)
Figure imgf000126_0001
Intermediate 14e was reacted with (5-tert-butyl-isoxazol-3-yl)-carbamic acid 2,2,2-trichloro-ethyl ester (WO 2006091671) in an analogous manner to that described in Example 14 step f to give the title compound. LCMS (Method 3): Rt: 5.43 min, m/z 717 [MH+].
b. l-(5-tert-Butyl-isoxazol-3-yl)-3-{(lS,4R)-4-[3-(4-hydroxymethyl- piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-urea (Intermediate 15b)
Figure imgf000126_0002
Intermediate 15a was reacted in an analogous manner to that described in Example 14 step g to give the title compound. LCMS (Method 3): Rt: 3.18 min, m/z 560 [MH+].
c. Methanesulfonic acid l-(6-{(lR,4S)-4-[3-(5-tert-butyl-isoxazol-3- yl)-ureido]-l,2,3,4-tetrahydro-naphthalen-l-yloxy}-[l,2,4]triazolo[4,3-a]pyridin- 3-yl)-piperidin-4-ylmethyl ester (Intermediate 15c)
Figure imgf000126_0003
Intermediate 15b was reacted in an analogous manner to that described in Example 14 step h to give the title compound. LCMS (Method 3): Rt: 3.46 min, m/z 638 [MH+].
d. l-(5-tert-Butyl-isoxazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Example 15)
Intermediate 15c was reacted with dimethylamine in an analogous manner to that described in Example 14 step i to give the title compound. LCMS (Method 5): Rt: 3.30 min, m/z 587.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.28 (9H, s), 1.33-1.47 (2H, m), 1.64-1.75 (1H, m), 1.75-1.84 (2H, m), 1.85-2.11 (4H, m), 2.13-2.20 (8H, m), 2.85-2.99 (2H, m), 3.35-3.47 (2H, m, obscured by water), 4.86-4.94 (1H, m), 5.56 (1H, t, J = 4.5 Hz), 6.41 (1H, s), 7.01 (1H, d, J = 8.4 Hz), 7.20 (1H, dd, J = 9.8, 2.2 Hz), 7.27- 7.34 (1H, m), 7.35-7.43 (3H, m), 7.62 (1H, d, J = 10.2 Hz), 7.65 (1H, d, J = 1.7 Hz), 8.20 (1H, s).
Example 16
l-(3-tert-Butyl-l'-methyl-l'H-[l,4,]bipyrazolyl-5-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt
Figure imgf000127_0001
a. l-(3-tert-Butyl-l'-methyl-l'H-[l,4,]bipyrazolyl-5-yl)-3-{(lS,4R)-4- [3-(4-triisopropylsilanyloxymethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin- 6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 16a)
Figure imgf000128_0001
Intermediate 2e was reacted with (3 -tert-butyl- 1 '-methyl- 1 Ή- [l ,4']bipyrazolyl-5-yl)-carbamic acid 2,2,2-trichloro-ethyl ester (US 6492529) in an analogous manner to that described in Example 14 step f to give the title compound. LCMS (Method 3): Rt: 5.04 min, m/z 795 [MH+].
b. l-(3-tert-Butyl-l'-methyl-l'H-[l,4,]bipyrazolyl-5-yl)-3-{(lS,4R)-4- [3-(4-hydr oxymethyl-piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 16b)
Figure imgf000128_0002
Intermediate 16a was reacted in an analogous manner to that described in
Example 14 step g to give the title compound. LCMS (Method 3): Rt: 2.96 min, m/z 639 [MH+].
c. Methanesulfonic acid l-(6-{(lR,4S)-4-[3-(3-tert-butyl-l'-methyl- l'H-[l,4']bipyrazolyl-5-yl)-ureido]-l,2,3,4-tetrahydro-naphthalen-l-yloxy}- [l,2,4]triazolo[4,3-a]pyridin-3-yl)-piperidin-4-ylmethyl ester (Intermediate 16c)
Figure imgf000128_0003
Intermediate 16b was reacted in an analogous manner to that described in Example 14 step h to give the title compound. LCMS (Method 3): Rt: 3.22 min, m/z 717 [MH+].
d. l-(3-tert-Butyl-l'-methyl-l'H-[l,4,]bipyrazolyl-5-yl)-3-{(lS,4R)-4- [3-(4-dimethylaminomethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt (Example 16)
Intermediate 16c was reacted with dimethylamine in an analogous manner to that described in Example 14 step i to give the title compound. LCMS (Method 5): Rt: 3.05 min, m/z 666.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.24 (9H, s), 1.33- 1.47 (2H, m), 1.63-1.75 (1H, m), 1.75-2.01 (4H, m), 2.01-2.1 1 (2H, m), 2.13 (2H, d, J = 7.5 Hz), 2.15 (6H, s), 2.87-2.98 (2H, m), 3.35-3.47 (2H, m, obscured by water), 3.87 (3H, s), 4.79-4.88 (1H, m), 5.56 (1H, t, J = 4.5 Hz), 6.27 (1H, s), 7.12-7.18 (2H, m), 7.26-7.41 (4H, m), 7.59-7.65 (3H, m), 8.00-8.04 (2H, m), 8.23 (0.6H, s).
Example 17
l-[3-tert-Butyl-l'-(2-dimethylamino-ethyl)-l 'H-[l,4,]bipyrazolyl-5-yl]-3- {4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-cyclohexyl}-urea
Figure imgf000129_0001
a. 4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-cyclohexylamine (Intermediate 17a)
Figure imgf000129_0002
Sodium hydride (60% in mineral oil, 0.24 g, 6.00 mmol) was added to a stirred solution of trans-4-amino cyclohexanol (0.24 g, 2.08 mmol) in anhydrous DMF (8 mL) at RT under an argon atmosphere. The reaction mixture was stirred at RT for 30 min, then Intermediate 3c (0.50 g, 2.01 mmol) in anhydrous DMF (7 mL) was added and stirred at 60 °C for 4 h. After cooling, the reaction mixture was quenched by careful addition of a saturated aqueous solution of NH4C1 and water (1 : 1) and extracted with DCM (3 x 50 mL). The combined organic layers were washed with water followed by brine, dried and concentrated in vacuo. The resultant residue was purified by FCC using 0-10% [2M NH3 in MeOH] in DCM to afford the title compound (0.61 g, 88%) as a brown oil. LCMS (Method 3): Rt 2.19 min, m/z 344 [MH+].
b. l-[3-tert-Butyl-l'-(2-hydroxy-ethyl)-l'H-[l,4']bipyrazolyl-5-yl]-3- {4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-cyclohexyl}-urea (Intermediate 17b)
Figure imgf000130_0001
A mixture of Intermediate Gc (0.75 g, 1.77 mmol), Intermediate 17a (0.61 g, 1.77 mmol) and DIPEA (0.46 mL, 2.64 mmol) in dioxane (10 mL) was stirred at 70 °C for 18 h. The volatiles were concentrated in vacuo and the resultant residue redissolved in DCM (50 mL), washed with water and brine then dried and evaporated to afford the title compound (1.35g, >100%). LCMS (Method 3): Rt 3.34 min, m/z 619 [MH+].
c. Methanesulfonic acid 2-[3-tert-butyl-5-(3-{4-[3-((2S,6R)-2,6- dimethyl-piperidin-l-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] -cyclohexyl}- ureido)-[l,4']bipyrazolyl-l'-yl]-ethyl ester (Intermediate 17c)
Figure imgf000131_0001
A mixture of Intermediate 17b (0.75 g, 1.21 mmol), methanesulfonyl chloride (140 μΐ,, 1.81 mmol) and DIPEA (0.63 mL, 3.62 mmol) in DCM (15 mL) was stirred at RT for 1 h. The reaction mixture was partitioned between DCM and water. The organic layer was washed with brine, separated through a phase separating cartridge and concentrated in vacuo to afford the title compound (0.82 g, 97%). LCMS (Method 3): Rt 3.59 min, m/z 697 [MH+].
d. l-[3-tert-Butyl-l'-(2-dimethylamino-ethyl)-l 'H-[l,4,]bipyrazolyl-5- yl]-3-{4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6- yloxy]-cyclohexyl}-urea (Example 17)
A mixture of Intermediate 17c (0.82 g, 1.17 mmol) and dimethylamine (2.0M in THF, 12 mL, 24 mmol) was stirred at 60°C for 18 h in a sealed vial. The volatiles were concentrated in vacuo and the resultant residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to afford the title compound (280 mg, 36%). LCMS (Method 5): Rt 3.43 min, m/z 646.4 [MH+]. Ή NMR (400 MHz, d6- DMSO): 1.23 (9H, s), 1.28-2.06 (14H, m), 2.19 (6H, s), 2.68 (2H, t, J = 6.5 Hz), 3.12-3.21 (1H, m), 3.43-3.54 (1H, m), 4.21 (2H, t, J = 6.5 Hz), 4.33-4.43 (1H, m), 6.18 (1H, s), 6.61 (1H, d J = 7.4 Hz), 7.18 (1H, dd, J = 9.8 2.2 Hz), 7.59 (1H, s), 7.63 (1H, dd, J = 9.7 0.6 Hz), 7.71-7.73 (1H, m), 7.92 (1H, s), 8.02 (1H, s), 8.18 (0.5H, s).
Example 18
l-(5-tert-Butyl)-2-p-tolyl-2H-pyrazol-3-yl)-3-{4-[3-((S)-2-methyl- piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] -cy clohexyl}-ur ea
Figure imgf000132_0001
a. 4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-cyclohexylamine (Intermediate 18a)
Figure imgf000132_0002
Sodium hydride (60% in mineral oil, 0.26 g, 6.39 mmol) was added to a stirred solution of trans-4-amino cyclohexanol (0.397 g, 3.45 mmol) in anhydrous DMF (10 mL) at RT under an argon atmosphere. The reaction mixture was stirred at RT for 30 min, then Intermediate 2d (0.50 g, 2.13 mmol) in anhydrous DMF (4 mL) was added and stirred at 60 °C for 10 h. After cooling, the reaction mixture was quenched by careful addition of a saturated aqueous solution of NH4C1 and water (1 : 1) and extracted with EtOAc and then DCM. The combined organic extracts were concentrated in vacuo and the resultant residue was purified by FCC, using 0-25% [2M NH3 in MeOH] in DCM, to afford the title compound (0.193 g, 27%) as an orange residue. LCMS (Method 3): Rt 2.00 min, m/z 330 [MH+].
b. l-(5-tert-Butyl)-2-p-tolyl-2H-pyrazol-3-yl)-3- {4- [3-((S)-2-methyl- piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] -cy clohexyl}-urea (Example
18)
A mixture of (5-tert-butyl-2-p-tolyl-2H-pyrazol-3-yl)-carbamic acid 2,2,2- trichloro-ethyl ester (73.7 mg, 0.182 mmol), Intermediate 18a (40 mg, 0.121 mmol) and DIPEA (63.2 μΕ, 0.363 mmol) in dioxane (2 mL) was stirred at 60 °C for 24 h. The volatiles were concentrated in vacuo and the resultant residue redissolved in DCM washed with water and brine then dried and evaporated to give a residue that was purified by MDAP to afford the title compound as a beige foam (45 mg, 63%). LCMS (Method 5): Rt 4.69 min, m/z 585.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 0.89 (3H, d J = 6.2 Hz), 1.25 (9H, s), 1.27-2.08 (14H, m), 2.36 (3H, s), 2.87-2.97 (1H, m), 3.1 1-3.20 (2H, m), 3.41-3.53 (1H, m), 4.04-4.1 1 (1H, m), 4.31-4.40 (1H, m), 6.25 (1H, s), 6.55 (1H, d, J = 7.4 Hz), 7.15 (1H, dd, J = 9.9 2.2 Hz), 7.28-7.35 (4H, m), 7.54 (1H, d, J = 1.6 Hz), 7.60 (1H, d, J = 9.7 Hz), 7.95 (1H, s).
Example 19
l-{5-tert-Butyl-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H-pyrazol-3-yl}- 3-{4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl}-urea
Figure imgf000133_0001
a. l-{5-tert-Butyl-2-[3-(2-hydroxy-ethoxy)-phenyl]-2H-pyrazol-3-yl}- 3-{4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl}-urea (Intermediate 19a)
Figure imgf000133_0002
A mixture of {5-tert-butyl-2-[3-(2-hydroxy-ethoxy)-phenyl]-2H-pyrazol-3- yl} -carbamic acid 2,2,2-trichloro-ethyl ester (WO 2007063868, 287.6 mg, 0.638 mmol), Intermediate 18a (140 mg, 0.425 mmol) and DIPEA (223 μΐ,, 1.28 mmol) in dioxane (10 mL) was stirred at 60 °C for 18 h. The volatiles were concentrated in vacuo and the resultant residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to afford the title compound (222 mg, 82%). LCMS (Method 3): Rt 3.35 min, m/z 631 [MH+].
b. Methanesulfonic acid 2-{3-[3-tert-butyl-5-(3-{4-[3-((S)-2-methyl- piperidin- 1-yl)- [1,2,4] triazolo [4,3-a] pyridin-6-yloxy] -cy clohexyl}-ur eido)- pyrazol-l-yl]-phenoxy}-ethyl (Intermediate 19b)
Figure imgf000134_0001
A mixture of Intermediate 19a (216 mg, 0.342 mmol), methanesulfonyl chloride (39.7 μί, 0.513 mmol) and DIPEA (179 μΐ,, 1.03 mmol) in DCM (5 mL) was stirred at RT for 30 min. The reaction mixture was partitioned between DCM and water. The organic layer was washed with brine, separated through a phase separating cartridge and concentrated in vacuo to afford the title compound (233 mg, 96%) as a cream foam. LCMS (Method 3): Rt 3.69 min, m/z 709 [MH+].
c. l-{5-tert-Butyl-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3-{4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin- 6-yloxy]-cyclohexyl}-urea (Example 19)
A mixture of Intermediate 19b (233 mg, 0.33 mmol) and dimethylamine (2.0M in THF, 1.65 mL, 3.30 mmol) was stirred at 60°C for 18 h in a sealed vial. The volatiles were concentrated in vacuo, the resultant residue was redissolved in EtOAc, washed with water and brine then dried and evaporated to give a residue that was purified by FCC using 0-10% [2M NH3 in MeOH] in DCM and then MDAP to afford the title compound (62 mg, 28%). LCMS (Method 5): Rt 3.38 min, m/z 658.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.26 (9H, s), 1.28-2.08 (14H, m), 2.25 (6H, s), 2.68 (2H, t, J = 5.7 Hz), 2.87-2.96 (1H, m), 3.1 1-3.19 (2H, m), 3.25-3.35 (2H, m), 3.43-3.53 (3H, m), 4.10 (2H, t, J = 5.7 Hz), 4.31-4.40 (1H, m), 6.26 (1H, s), 6.58 (1H, d, J = 7.5 Hz), 6.93-6.97 (1H, m), 7.01-7.07 (2H, m), 7.15 (1H, dd, J = 9.8 2.2 Hz), 7.39 (1H, t, J = 8.0 Hz), 7.54 (1H, d, J = 1.5 Hz), 7.60 (1H, t, J = 9.9 Hz), 8.03 (1H, s), 8.17 (1H, s).
Example 20
l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]- 2H-pyrazol-3-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydronaphthalen-l-yl}-urea
Figure imgf000135_0001
a. 3-Hydrazinophenol (Intermediate 20a)
Figure imgf000135_0002
To a stirred, ice cooled solution of 3-aminophenol (2.00 g, 18.3 mmol) in concentrated HC1 (20 mL) was added dropwise a solution of sodium nitrite (1.33 g, 19.24 mmol) in H20 (10 mL), ensuring the internal temperature did not exceed 10°C. After 10 min, a solution of tin (II) chloride (7.645 g, 40.3 mmol) in concentrated HC1 (10 mL) was added dropwise, and the ice bath removed. After stirring at RT for 45 min, the reaction was basified to pH 10 with careful addition of 10 M NaOH solution (50 mL), and then extracted with EtOAc (3 x). The organic extracts were dried over MgSC^, concentrated in vacuo and triturated with Et20 to afford the title compound as a brown solid (353 mg, 16%). 1H NMR (300 MHz, d6- DMSO): 3.83 (2H, br s), 5.96-6.02 (1H, m), 6.15-6.21 (1H, m), 6.22 (1H, t, J = 2.2 Hz), 6.52 (1H, br s), 6.84 (1H, t, J = 8.0 Hz), 8.91 (1H, br s).
b. 2-[5-Amino-l-(3-hydroxy-phenyl)-lH-pyrazol-3-yl]-2-methyl- propionitrile (Intermediate 20b)
Figure imgf000136_0001
2,2-Dimethyl-3-oxo-pentanedinitrile (WO 2008/034008, 353 mg, 2.80 mmol) and Intermediate 20a (387 mg, 2.80 mmol) were suspended in EtOH (10 mL) and concentrated HCl was added (71 1 μΐ,, 8.53 mmol) and the reaction was refluxed for 90 min. The reaction was cooled, concentrated in vacuo and partitioned between H20 and EtOAc. The organic layer was separated, dried over MgSC^ and concentrated in vacuo and the resulting oil was triturated with pentane / Et20 (9 : 1) to afford the title compound as a brown solid (363 mg, 53%). LCMS (Method 3): Rt 2.77 min, m/z 243.3 [MH+].
c. 2-(5-Amino-l-{3-[2-(tetrahydro-pyran-2-yloxy)-ethoxy]-phenyl}-lH- pyrazol-3-yl)-2-methyl-propionitrile (Intermediate 20c)
Figure imgf000136_0002
To an ice cooled solution of Intermediate 20b (363 mg, 1.50 mmol), 2- (tetrahydro-2H-pyran-2-yloxy)ethanol (329 mg, 305 μί, 2.25 mmol) and triphenylphosphine (786 mg, 3.00 mmol) in anhydrous THF (10 mL) was added dropwise diisopropyl azodicarboxylate (606 mg, 590 μί), and the ice bath removed and stirring continued under an Ar atmosphere. After 3 h, a further 1.0 eq of diisopropyl azodicarboxylate was added and the reaction stirred overnight. The reaction mixture was then concentrated in vacuo and subjected to FCC, eluting with 5 - 50% EtOAc in cyclohexane. The isolated product was repurified using FCC, eluting with 2.5 - 7.5% Et20 in DCM, to afford the title compound as a yellow oil (317 mg, 57%). LCMS (Method 3): Rt 3.67 min, m/z 393.3 [M+Na].
d. (5-(Cyano-dimethyl-methyl)-2-{3-[2-(tetrahydro-pyran-2-yloxy)- ethoxy]-phenyl}-2H-pyrazol-3-yl)-carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate 20d)
Figure imgf000137_0001
Intermediate 20c (317 mg, 0.86 mmol) was dissolved in EtOAc (2.5 mL) and 1M NaOH solution (1.4 mL, 2.14 mmol) and cooled in an ice bath. While stirring, 2,2,2-trichloroethyl chloroformate (227 mg, μΕ, 1.07 mmol) was then added dropwise, and the ice bath removed. After 30 min, a further 0.65 eq. of 2,2,2- trichloroethyl chloroformate was added, and the reaction continued. After 30 min, a further 0.65 eq. of 2,2,2-trichloroethyl chloroformate was added, and the reaction continued overnight. The reaction was then partitioned between H20 and EtOAc, the organic layer was separated, and the aqueous layer extracted again with EtOAc. The combined organics were dried over MgS04, concentrated in vacuo and subjected to FCC, eluting with 5 - 30%> EtOAc in cyclohexane, to afford the title compound as a light yellow oil (375 mg, 80%). LCMS (Method 3): Rt 4.41 min, m/z 567, 569 [M+Na].
e. {5-(Cyano-dimethyl-methyl)-2-[3-(2-hydroxy-ethoxy)-phenyl]-2H- pyrazol-3-yl}-carbamic acid 2,2,2-trichloro-ethyl ester (Intermediate 20e)
Figure imgf000138_0001
Intermediate 20d (375 mg, 0.69 mmol) was dissolved in MeOH (5 niL) and loaded onto a 20 g SCX-2 SPE cartridge (pre-conditioned with MeOH). The cartridge was then washed with MeOH (2 column volumes) and the eluent concentrated in vacuo to afford the title compound as a colourless oil (283 mg, 89%). LCMS (Method 3): Rt 3.71 min, m/z 461 , 463 [MH+].
f. l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-hydroxy-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 20f)
Figure imgf000138_0002
The title compound was prepared using Intermediate 20e and Intermediate 2 in an analogous fashion to the procedure described for Intermediate A step d. LCMS (Method 3): Rt 3.39 min, m/z 690.4 [MH+].
g. Methanesulfonic acid 2-{3-[3-(cyano-dimethyl-methyl)-5-(3-{(lS,4R)-4- [3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4- tetrahydro-naphthalen-l-yl}-ureido)-pyrazol-l-yl]-phenoxy}-ethyl ester (Intermediate 20g)
Figure imgf000139_0001
The title compound was prepared using Intermediate 20f in an analogous fashion to the procedures described for Intermediate A step e. LCMS (Method 3): Rt 3.64 min, m/z 768.5 [MH+].
l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]- 2H-pyrazol-3-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydronaphthalen-l-yl}-urea (Example 20)
The title compound was prepared using Intermediate 20g using the general displacement procedure described for Example 1. LCMS (Method 5): Rt 3.46 min, m/z 717.5 [MH+].
Example 21
l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]- 2H-pyrazol-3-yl}-3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea
Figure imgf000139_0002
a. l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-hydroxy-ethoxy)-ph pyrazol-3-yl}-3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Intermediate 21a)
Figure imgf000140_0001
The title compound was prepared using Intermediate 20e and Intermediate 3 in an analogous fashion to the procedure described for Intermediate A step d. LCMS (Method 3): Rt 3.61 min, m/z 704.5 [MH+].
b. Methanesulfonic acid 2-{3-[3-(cyano-dimethyl-methyl)-5-(3-{(lS,4R)-4- [3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-ureido)-pyrazol-l-yl]-phenoxy}-ethyl ester (Intermediate 21b)
Figure imgf000140_0002
The title compound was prepared using Intermediate 21a in an analogous fashion to the procedures described for Intermediate A step e. LCMS (Method 3): Rt 3.85 min, m/z 782.5 [MH+].
l-{5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]- 2H-pyrazol-3-yl}-3-{(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)- [1,2,4] triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea (Example 21)
The title compound was prepared using Intermediate 21b using the general displacement procedure described for Example 1. LCMS (Method 3): Rt 2.83 min, m/z 731.5 [MH+].
Intermediate 22
[5-tef"f-Butyl-2-(2-methoxyethyl)-2H-pyrazol-3-yl] carbamic acid 2,2,2- trichloroethyl ester
Figure imgf000141_0001
a. 5-terf-Butyl-2-(2-methoxyeth l)-2H-pyrazol-3-ylamine (Intermediate 22b)
Figure imgf000141_0002
A stirred suspension of (2-methoxyethyl) hydrazine oxalic acid (0.50 g, 2.77 mmol) and 4,4-dimethyl-3-oxopentanenitrile (0.347 g, 2.77 mmol) in ethanol (6 mL) was heated at 85 °C for 4.5 h during which time all solids dissolved. The reaction mixture was allowed to cool overnight and then the solvent was evaporated. The residue was dissolved in dichloromethane (20 mL) and washed with saturated aqueous NaHC03 (10 mL). The organic layer was separated using a phase separator cartridge and evaporated. The residual oil was dissolved in diethyl ether and evaporated to afford the title compound (0.538 g, 98%) as a solid. Ή NMR (300 MHz, CDC13): 1.26 (9H, s), 3.33 (3H, s), 3.67 (2H, t, J 4.3 Hz), 3.95 (2H, br s), 4.13 (2H, t, J 4.6 Hz), 5.36 (1H, s). b. [5-terf-Butyl-2-(2-methoxy-ethyl)-2H-pyrazol-3-yl] carbamic acid 2,2,2- trichloroethyl ester (Intermediate 22)
Figure imgf000142_0001
To a stirred mixture of Intermediate 22a (0.535 g, 2.71 mmol) in ethyl acetate (4 mL) and 1 M aqueous NaOH (4.9 mL, 4.90 mmol) was added 2,2,2-trichloroethyl chloroformate (0.40 mL, 2.90 mmol) and the mixture was stirred at room temperature for 1.5 h. The mixture was diluted with water (50 mL) and extracted with ethyl acetate (2 x 25 mL). The combined organic extracts were washed with water and saturated aqueous NaCl, then evaporated. The residual oil was dissolved in diethyl ether and evaporated to give the title compound (1.06 g) as an oil. LCMS (Method 3): Rt 4.17 min, m/z 372/374/376 [MH+]; Ή NMR (300 MHz, CDC13): 1.30 (9H, s), 3.44 (3H, s), 3.74 (2H, t, J 4.3 Hz), 4.29 (2H, t, J 4.3 Hz), 4.82 (2H, s), 6.26 (1H, s), 8.93 (1H, s).
Intermediate 23
[5-terf-Butyl-2-propyl-2H-pyrazol-3-yl] carbamic acid 2,2,2-trichloroethyl ester
Figure imgf000142_0002
a. 5-tef"f-Butyl-2-propyl-2H-pyrazol-3- lamine (Intermediate 23a)
Figure imgf000142_0003
A stirred solution of propylhydrazine hydrochloride (0.50 g, 4.52 mmol) and 4,4- dimethyl-3-oxopentanenitrile (0.566 g, 2.77 mmol) in ethanol (6 mL) was treated with coned. HCl (1 drop) and heated at 85 °C for 4.5 h. The reaction mixture was allowed to cool overnight and then the solvent was evaporated. The residue was dissolved in dichloromethane (20 mL) and washed with saturated aqueous NaHC03 (10 mL). The organic layer was separated using a phase separator cartridge and evaporated. The residual oil was dissolved in diethyl ether and evaporated to afford the title compound (0.776 g, 95%) as an oil that slowly solidified. Ή NMR (300 MHz, CDC13): 0.92 (3H, t, J 7.4 Hz), 1.25 (9H, s), 1.87 - 1.79 (2H, sextet, J 7.4 Hz), 3.35 (2H, br s), 3.85 (2H, t, J 7.4 Hz), 5.41 (1H, s). b. [5-tef"f-Butyl-2-propyl-2H-pyrazol-3-yl]carbamic acid 2,2,2-trichloroethyl ester (Intermediate 23)
Figure imgf000143_0001
To a stirred mixture of Intermediate 23a (0.774 g, 4.27 mmol) in ethyl acetate (4 mL) and 1 M aqueous NaOH (7.7 mL, 7.7 mmol) was added 2,2,2-trichloroethyl chloro formate (0.62 mL, 4.50 mmol) and the mixture was stirred at room temperature for 1.5 h. The mixture was diluted with water (50 mL) and extracted with ethyl acetate (2 x 25 mL). The combined organic extracts were washed with water and saturated aqueous NaCl, then evaporated to give the title compound (1.57 g) as an oil. LCMS (Method 3): Rt
4.28 min, m/z 356/358/360 [MH ]. Ή NMR (300 MHz, CDC13): 0.90 (3H, t, J 7.5 Hz), 1.28 (9H, s), 1.78-1.87 (2H, m), 3.92 (2H, t, J 7.4 Hz), 4.83 (2H, s), 6.08 (1H, s), 6.46
(1Η, s).
[5-terf-Butyl-2-ethyl-2H-pyrazol-3-yl]carbamic acid 2,2,2-trichloroethyl ester
Figure imgf000143_0002
To a stirred mixture of 3-tert-butyl-l -ethyl- lH-pyrazole-5-amine (0.500 g, 2.99 mmol) in ethyl acetate (7.5 mL) and 1 M aqueous NaOH (7.5 mL, 7.5 mmol), cooled in an ice bath, was added 2,2,2-trichloroethyl chloroformate (0.453 mL, 3.29 mmol) and the mixture was stirred for 45 min. The mixture was diluted with water and extracted twice with ethyl acetate. The combined organic extracts were dried (MgS04) and evaporated. The residue was crystallised from pentane-diethyl ether (1 : 1) to give the title compound (0.078 g) as a white solid. The mother liquors were evaporated and purified by column chromatography (0-40% EtOAc/cyclohexane) to give the title compound (0.817 g) as a colourless oil (Total yield 0.895 g, 87%). LCMS (Method 3): Rt 4.09 min, m/z 342/344/346 [MH+]. Ή NMR (300 MHz, CDC13): 1.44 (12H, m), 4.78-4.83 (2H, m), 4.88 (2H, s), 6.53 (1H, s), 9.82 (1H, s).
Intermediate I
(1 S,4R)-4- {3- [4-(2-Dimethylamino-ethoxy)-piperidin- 1-yl] - [ 1 ,2,4] triazolo [4,3- a]pyridin-6-yloxy}-l,2,3,4-tetrahydro-naphthalen-l-ylamine
Figure imgf000144_0001
e. 3-Chloro-6-fluoro-[l,2,4]triazolo[4,3-a]pyridine (Intermediate la)
Figure imgf000144_0002
Intermediate 14a (908 mg, 6.60 mmol) was dissolved in DCM (50 mL) and N- bromosuccinimide (1.29 g, 7.26 mmol) added. The reaction was heated to 45 °C for 4 h, then cooled and partitioned between DCM (150 mL) and saturated aqueous NaHC03 (150 mL), and extracted into DCM (3 x). The combined organic layers were washed with brine, dried (MgS04) and evaporated in vacuo. Purification by FCC, using 0-5%> [2M NH3 in MeOH] in DCM, gave the title compound (900 mg, 63%). LCMS (Method 1): Rt 1.94 min, m/z 216/218 [MH+]. b. {2- [ l-(6-Fluoro- [ 1 ,2,4] triazolo [4,3-a] yridin-3-yl)-piperidin-4-yloxy] - ethyl}-dimethyl-amine (Intermediat lb)
Figure imgf000145_0001
A solution of Intermediate la (435 mg, 2.01 mmol) and Dimethyl- [2-(piperidin- 4-yloxy)-ethyl]-amine (1.09 g, 6.01 mmol) in DMA (6.0 mL) was heated in the microwave at 180°C for 6 h. The cooled mixture was applied to an SCX-2 cartridge (20 g), washing with methanol then eluting basic components with 2 M N¾ in MeOH. Product containing fractions were combined and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to give the title compound as yellow oil (570 mg, 92%). LCMS (Method 4): Rt 1.61 and 0.41 min, m/z 308 [MH+]. c. (lS,4R)-4-{3-[4-(2-Dimethylamino-ethoxy)-piperidin-l-yl]- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy}- 1 ,2,3,4-tetrahydro-naphthalen- 1-ylamine (Intermediate I)
Figure imgf000145_0002
To a solution of Intermediate 1 (318 mg, 1.95 mmol) in DMF (8 mL) was added NaH (60% dispersion in oil, 223 mg, 5.57 mmol) and the mixture stirred at RT for 15 min. A solution of Intermediate lb (570 mg, 1.85 mmol) in DMF (7 mL) was added and the mixture stirred at RT for 18 hours. The cooled mixture was diluted with water and extracted with DCM (5 x 20 mL). The combined organics were dried and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM, to give the title compound as a brown gum (294 mg, 34%). LCMS (Method 4): Rt 1.65 min and 0.42 min, m/z 451 [MH+].
Intermediate J (WO2013083604)
(lS,4R)-4-[3-((R)-3-Triisopropylsilanyloxymethyl-piperidin-l-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin- -yloxy] - 1 ,2,3,4- tetrahydro-nap hthalen- 1-ylamine
Figure imgf000146_0001
Intermediate K (WO2013083604)
(lS,4R)-4-[3-((S)-3-Triisopropylsilanyloxymethyl-piperidin-l-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin- -yloxy] - 1 ,2,3,4- tetrahydro-nap hthalen- 1-ylamine
Figure imgf000146_0002
General urea formation procedure (GP1)
A solution of the appropriate LHS (Ex.20-35) (0.273 mmol), the appropriate Intermediate 14e or Intermediate I-K (0.273 mmol) and DIPEA (0.409 mmol) in dioxane (2 mL) was heated at 60°C for 48 h. The reaction mixture was cooled and diluted with DCM (5 mL). The organic layer was washed with water (3 x 5 mL), passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0- 10%) [2M NH3 in MeOH] in DCM to afford the desired urea intermediate or Examples 22-27 (40-80%) General procedure for TBAF deprotection, mesylation and displacement
(GP2)
Tetrabutylammonium fluoride (1M solution in THF, 0.211 mmol) was added to a solution of the appropriate Triisopropylsilanyloxymethyl-piperidin-intermediate (Ex. 30-37) (0.151 mmol) in THF (3 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (2 x 5 mL) and brine (5 mL). The organic layer was passed through a phase separator and concentrated in vacuo. The residue was purified by FCC, using 0-10% [2M NH3 in MeOH] in DCM to afford the desired hydroxyl-intermediate (60-80%>) Methanesulfonyl chloride (0.13 mmol) was added to a solution of the appropriate
Hydroxyl-intermediate (0.08 mmol) and DIPEA (0.26 mmol) in DCM (1.5 mL) and the reaction mixture was stirred at RT for 1 h. The mixture was diluted with DCM (5 mL) and washed with water (3 x 5 mL). The organic layer was passed through a phase separator and concentrated in vacuo to afford the desired mesylate intermediate in quantitative yields.
A mixture of the appropriate Mesylate intermediate (0.14 mmol) and an appropriate amine [see table below] (2.15 mmol) in anhydrous THF (3 mL) was stirred at 40 °C for 18 h in a sealed vial. The volatiles were concentrated in vacuo and the resultant residue was purified by HPLC (Gemini CI 8, 20-40% MeCN in H20, 0.1% HC02H, 18ml/min.) and freeze dried to afford the Examples 28-37 (30-50%). o
o
CD
Figure imgf000148_0001
OZda/X3d 00t7S6l/M0Z OAV (ds-DMSO): 1.21 (9H, s), 1.73-1.85 (2H, m), 1.85-
2.12 (5H, m), 2.13-2.22 (IH, m), 2.54 (IH, s), 2.80
(3H, s), 2.81 (3H, s), 3.02-3.11 (2H, m), 3.63-3.70
(3H, m), 3.77-3.82 (2H, m), 3.98 (2H, t, J 5.9 Hz), (Method 5
N/A GP1 I l-[5-tert-Butyl-2-(2-hydroxy-ethyl)- 4.83-4.90 (IH, m), 5.59 (IH, t, J 4.2 Hz), 6.07 (IH, Rt 2.91
23
HO 2H-pyrazol-3-yl]-3-((l S,4R)-4- {3- s), 7.20 (IH, d, J 8.7 Hz), 7.23-7.33 (2H, m), 7.35- mins, m/z
[4-(2-dimethylamino-ethoxy)- 7.43 (3H, m), 7.66 (IH, d, J 9.9 Hz), 7.74 (IH, s), 660 [MH+
(WO piperidin-l-yl]-[l,2,4]triazolo[4,3- 8.47 (IH, br s) plus 4 protons obscured by solvent
2013083604) a]pyridin-6-yloxy} - 1 ,2,3,4- peaks
tetrahydro-naphthalen- 1 -yl)-urea
hydrogen chloride salt
(continu
(ds-DMSO): 1.24 (9H, s), 1.72-1.92 (3H, m), 1.92-
2.17 (5H, m), 2.54 (IH, s), 2.80 (6H, s), 3.01-3.11
(2H, m), 3.25-3.30 (IH, m), 3.36-3.45 (2H, m),
3.62-3.70 (IH, m), 3.78 (2H, t, J 5.3 Hz), 3.87 (3H,
Figure imgf000150_0001
(Method 5
N/A GP1 I 1 -(3 -tert-Butyl- 1 '-methyl- 1 Ή- s), 4.80-4.88 (IH, m), 5.57 (IH, t, J 4.4 Hz), 6.26 Rt 3.1
24
[l,4']bipyrazolyl-5-yl)-3-((l S,4R)-4- (IH, s), 7.17 (2H, dd, J 9.8, 2.0 Hz), 7.27-7.33 (IH, mins, m
(US 6492529)
{3-[4-(2-dimethylamino-ethoxy)- m), 7.33-7.37 (2H, m), 7.38-7.42 (IH, m), 7.62 696 [MH+ piperidin-l-yl]-[l,2,4]triazolo[4,3- (2H, t, J 4.5 Hz), 7.70 (IH, d, J 1.7 Hz), 8.02 (IH,
a]pyridin-6-yloxy} - 1 ,2,3,4- s), 8.05 (IH, s), 9.48 (IH, br s)
tetrahydro-naphthalen- 1 -yl)-urea
hydrogen chloride salt
(continu
(ds-DMSO): 1.23 (9H, s), 1.69-1.79 (2H, m), 1.80- 1.90 (IH, m), 1.92-2.05 (4H, m), 2.08-2.15 (IH,
m), 2.75 (3H, s), 2.76 (3H, s), 3.02 (2H, t, J 10.0
Figure imgf000151_0001
Hz), 3.22-3.34 (4H, m), 3.59-3.64 (IH, m), 3.74 (Method 5
(WO N/A GP1 l-(5-tert-Butyl-isoxazol-3-yl)-3- (2H, t, J 5.1 Hz), 4.83-4.89 (IH, m), 5.55 (IH, t, J
25 Rt 3.40 206091671) ((1 S,4R)-4- {3-[4-(2-dimethylamino- 4.1 Hz), 6.35 (IH, s), 7.03 (IH, d, J 8.8 Hz), 7.22- mins, m/ ethoxy)-piperidin- 1 -yl] - 7.29 (2H, m), 7.32-7.34 (2H, m), 7.37 (IH, d, J 7.7 617 [MH+ [l,2,4]triazolo[4,3-a]pyridin-6- Hz), 7.62 (IH, d, J 10.1 Hz), 7.71 (IH, s), 9.33
yloxy} -l,2,3,4-tetrahydro- (IH, s), 9.63 (IH, br s)
naphthalen-l-yl)-urea hydrogen
chloride salt
(continu
Figure imgf000152_0001
(continu
Figure imgf000153_0001
hydrogen chloride salt
(continu
(ds-DMSO): 1.21 (9H, s), 1.79-2.22 (8H, m), 2.39
(IH, m), 2.80-2.83 (4H, m), 2.85-2.90 (IH, m),
Figure imgf000154_0001
1 -(5-tert-Butyl-2-methyl-2H- 2.95-3.04 (2H, m), 3.07-3.35 (3H, m, partially (Method 5
GP1, pyrazol-3-yl)-3- {(1 S,4R)-4-[3-((S)- obscured by water), 3.61 (3H, s), 3.70 (IH, m), Rt 2.
2-
28 I (US 2004 GP2 3- {[(2-hydroxy-ethyl)-methyl- 3.75 (2H, t, J 5.0 Hz), 4.88 (lH, m), 5.69 (lH, m), mins, m
Methyl
192653) amino]-methyl} -piperidin-l-yl)- 6.06 (IH, s), 7.10 (IH, d, J 8.5 Hz), 7.29-7.33 (IH, 630 [MH+ amino- [l,2,4]triazolo[4,3-a]pyridin-6- m), 7.37-7.48 (4H, m), 7.78 (IH, d, J 9.9 Hz), 7.91
ethanol
yloxy] - 1 ,2,3 ,4-tetrahydro- (IH, d, J 5.8 Hz), 8.65 (IH, br s), 9.50 (IH, br s).
naphthalen- 1 -yl} -urea hydrogen
chloride salt
(continu
Figure imgf000155_0001
chloride salt
(continu
Figure imgf000156_0001
naphthalen- 1 -yl} -urea
(continu
(ds-DMSO): 1.20 (9H, s), 1.45-1.64 (2H, m),
1.75-2.22 (7H, m), 2.27-2.90 (2H, m), 2.92- 3.07 (3H, m), 3.08-3.25 (2H, m), 3.22-3.38
Figure imgf000157_0001
(Method 5):
(2H, m, obstructed by water), 3.40-3.53 (2H,
1 -(5-tert-Butyl-2-methyl-2H- Rt 2.88 mins,
GP1, m), 3.58 (3H, s), 3.70-3.83 (2H, m), 4.82-4.91
pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(4-
(US 2004 2- 14e m/z
GP2 (1H, m), 5.34 (1H, bs), 5.54-5.60 (1H, m),
Methyl {[(2-hydroxy-ethyl)-methyl-amino]
192653) 630.4[MH+].
6.02 (1H, s), 6.97-7.04(lH, m), 7.20 (1H, dd,
amino- methyl} -piperidin- 1 -yl)- J 2.37, 9.89 Hz), 7.24-7.44 (4H, m), 7.34 (1H,
ethanol [l,2,4]triazolo[4,3-a]pyridin-6- d, J 9.89 Hz), 7.67-7.70 (1H, m), 8.44 (1H,
yloxy] - 1 ,2,3 ,4-tetrahydro- bs).
naphthalen- 1 -yl} -urea hydrogen
chloride salt
(continu
Figure imgf000158_0001
Figure imgf000159_0001
(CDC13): 1.28 (9H, s), 1.70-2.36 (9H, m), 2.59
Figure imgf000160_0001
(3H, br s), 2.67-3.04 (6H, m), 3.21 (IH, m),
1 -(5-tert-Butyl-2-methyl-2H- 3.49 (IH, d, J 10.4 Hz), 3.75 (5H, s), 5.14 (IH,
GP1,
2- pyrazol-3-yl)-3- {(1 S,4R)-4-[3-((R) (Method 5):
K m), 5.23 (IH, s), 6.14 (IH, s), 6.45 (IH, br s),
(US 2004 GP2
Methyl 3- {[(2-hydroxy-ethyl)-methyl- Rt 2.98 mins,
7.09 (IH, dd, J 9.9, 1.5 Hz), 7.20 (2H, d, J 4.1
192653)
amino- amino]-methyl} -piperidin-l-yl)- m/z 630
Hz), 7.32 (IH, m), 7.40 (IH, s), 7.51-7.56 (2H,
ethanol [l,2,4]triazolo[4,3-a]pyridin-6- [MH+].
m), 7.61 (IH, br s).
yloxy] - 1 ,2,3 ,4-tetrahydro- naphthalen- 1 -yl} -urea hydrogen
chloride salt
(continu
Figure imgf000161_0001
naphthalen- 1 -yl} -urea
(continu
Figure imgf000162_0001
naphthalen-l-yl}-urea formate salt
(continu
Figure imgf000163_0001
Example 38 l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((2S,4S)-4- dimethylamino- l-methyl-pyrrolidin-2-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea hydrochloride salt
Figure imgf000164_0001
HCI g. (2S,4S)-4-Dimethylamino-l-methyl-pyrrolidine-2-carboxylic
(Intermediate 38a)
Figure imgf000164_0002
A solution of l-methyl-4-amino-L-proline carboxylic acid (5.0 g, 34.2 mmol) in trifluoroethanol (40 mL) was treated with paraformaldehyde (5.13 g, 171 mmol). The reaction mixture was stirred for 15 min. at RT and NaBH4 was added to the mixture. The reaction mixture was then heated for 2 hours at 90°C. The reaction was quenched with MeOH and the volatiles were evaporated to dryness under reduced pressure, affording the title compound as an off white foam that was used without further purification (6.0 g, 99%). LCMS (Method 3): Rt 0.43, m/z 173[MH ]. h. (2S,4S)-4-Dimethylamino-l-methyl-pyrrolidine-2-carboxylic acid N'-(5- fluoro-pyridin-2-yl)-hydrazide (Intermediate 38b)
Figure imgf000164_0003
A solution of Intermediate 38a (5.95 g, 34.2 mmol) in DCM (60 mL) was treated with EDCI (7.87 g, 34.2 mmol), HOBt (0.462 g, 41.0 mmol) and (5-fiuoro-pyridin-2-yl)- hydrazine (4.35g g, 34.2 mmol) and the solution was stirred at RT for 16 hours. The reaction mixture was partitioned between H20 (50 mL) and DCM (40 mL) and the phases were separated. The organic phase was washed with sat. bicarbonate solution and the brine. The volatiles were concentrated in vacuo and the resultant residue was purified by FCC using 0-10% MeOH in DCM to afford the title compound (1.74 g, 20%). LCMS (Method 3): Rt 0.44 min, m/z 282 [MH+]. i. [(3 S,5S)-5-(6-Fluoro- [ 1 ,2,4] triazolo [4,3-a] pyridin-3-yl)- 1-methyl- pyrrolidin-3-yl]-dimethyl-amine (Intermediate 38c)
Figure imgf000165_0001
A solution of Intermediate 38b (1.73 g, 6.15 mmol) in THF (40 mL) was treated with triphenyl phosphine (3.22 g, 12.3 mmol) and TEA (3.43 mL, 24.6 mmol). Hexachloroethane (2.91 g, 12.3 mmol) was added to the solution portion wise over a period of 5 minutes. The reaction mixture was heated at 60°C for 16 hours The reaction mixture was cooled down, treated with acetone to precipitate the triethylammonium chloride, which was then filtered; the filter cake was washed abundantly with acetone and the solvent was evaporated under reduced pressure. The residue was purified by SCX-2 cartridge eluting with DCM (40 mL), MeOH (40 mL) and 2M NH3 in MeOH (40 mL). The product containing fractions were evaporated under reduced pressure to give the title compound as a brown oil (1.3 g, 80%). Ή NMR (300 MHz, d6-DMSO): 201-2.13 (1H, m), 2.2 (3H, s), 2.29 (6H, s), 2.31-2.33 (1H, m), 2.34-2.39 (1H, m), 2.51-2.61 (1H, m), 2.88-2.98 (1H, m), 3.31-3.39 (1H, dd), 3.50 (1H, s), 4.17-4.27 (1H, m), 7.14-7.24 (1H, m), 7.67-7.77 (1H, m), 9.00-9.07 (1H, m). j. {(3S,5S)-5-[6-((lR,4S)-4-Amino-l,2,3,4-tetrahydro-naphthalen-l-yloxy)- [ 1 ,2,4] triazolo [4,3-a] pyridin-3-yl] - l-methyl-pyrrolidin-3-yl}-dimethyl-amine
(Intermediate 38d)
Figure imgf000166_0001
A suspension of sodium hydride (60% dispersion in oil, 548 mg, 14.6 mmol) in dry DMF(10.0 mL) under nitrogen was treated with Intermediate 1 (800 mg, 4.9 mmol) and the mixture was stirred for 20 min. Intermediate 38c (1.28 g, 4.6 mmol) was added and the mixture was stirred at RT for 4 hours then quenched with MeOH. The mixture was further diluted with MeOH and applied to a pre-conditioned (MeOH) SCX-2 cartridge (25 g). The cartridge was eluted with MeOH then 2M NH3 in MeOH. The relevant product containing fractions were combined and concentrated in vacuo to give the title compound as a dark brown oil (1.8 g, 86%). LCMS (Method 3): Rt 0.6, m/z 407 [MH+]. k. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((2S,4S)-4- dimethylamino- l-methyl-pyrrolidin-2-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - l,2,3,4-tetrahydro-naphthalen-l-yl}-urea hydrochloride salt (Example 38)
(5-Tert-butyl-2-methyl-2H-pyrazol-3-yl)-carbamic acid 2,2,2-trichloro-ethyl ester (US20040192653; 242 mg, 0.74 mmol) was treated with a solution of Intermediate 38d (300 mg, 0.74 mmol) in 1 ,4-dioxane (5 mL) then DIPEA (185
Figure imgf000166_0002
1.1 mmol) was added. The mixture was stirred at 50 °C overnight. The mixture was evaporated in vacuo and the residue purified by HPLC (Method 6, 20-40% MeCN in H20, 0.1% HC02H over 30 min) to give a colourless glass that was dissolved in MeCN and treated with IN HC1 (550 μί, 1 eq.). The volatiles were evaporated to dryness under reduced pressure affording a colourless glass that upon trituration with ether gave a solid. The solid was collected by filtration, washed with ether and dried at 50 °C in vacuo to give the title compound as an off white solid (346 mg, 74%). LCMS (Method 5): Rt 3.01 min, m/z 586.4 [MH+]. Ή NMR (400 MHz, d6-DMSO): 1.20 (9H, s), 1.81-2.15 (5H, m), 2.16-2.24 (2H, bs), 2.42- 2.52 (4H, br s, partially obscured by DMSO), 2.74-2.80 (6H, s), 3.45-3.53 (2H, m), 3.56 (3H, s), 3.97-4.06 (1H, m), 4.79-4.88 (1H, m), 5.54 (1H, t, J 4.3 Hz), 6.00 (1H, s), 7.08 (1H, d, J 8.4 Hz), 7.22-7.27 (1H, m), 7.33-7.39 (4H, m), 7.77 (1H, d, J 9.3 Hz), 8.45 (1H, bs), 8.60 (1H, s).
Example 39 l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(l,4-dimethyl- 1 ,4,9-triaza-spir o [5.5] undec-9-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - 1 ,2,3,4- tetrahydro-naphthalen-l- l}-urea hydrochloride salt
Figure imgf000167_0001
a. 9-(6-Fluoro- [ 1 ,2,4] triazolo [4,3-a] pyridin-3-yl)- 1 ,4-dimethyl- 1 ,4,9-triaza- spiro[5.5]undecane (Intermediate 39a
Figure imgf000167_0002
A mixture of Intermediate la (713 mg, 3.30 mmol), l,4-Dimethyl-l,4,9-triaza- spiro[5.5]undecane (2.93g, 10.0 mmol) and DIPEA (6.1 mL, 35.0 mmol) in DMA (6 mL), under nitrogen was stirred at RT for 5 min, then treated with microwave irradiation at 170°C for 6 h. The RM was concentrated in vacuo and the residue was purified by FCC, using 0-10% MeOH in DCM, then 0-20% [2M NH3 in MeOH] in DCM to give a dark brown gum. This was re-purified by FCC, using 0-20% [2M NH3 in MeOH] in DCM to afford the title compound (520 mg, 50%>), as a brown gum. LCMS (Method 3): Rt 0.38/0.42 min, m/z 319. [MH+]. b. (lS,4R)-4-[3-(l,4-Dimethyl-l,4,9-triazo-spiro[5.5]undec-9-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - 1 ,2,3,4- tetrahydro-nap hthalen- 1-ylamine (Intermediate 39b)
Figure imgf000168_0001
Sodium hydride (200 mg, 5.0 mmol) was added to a mixture of Intermediate 1
(269 mg, 1.65 mmol) in anhydrous DMF (3 mL) and the RM was stirred at RT under an argon atmosphere for 30 min. Intermediate 39a (515 mg, 1.62 mmol) was added and the RM was stirred at RT for 22.5 h. The reaction mixture was diluted with water (10 mL) and extracted with DCM (5 x 20 mL). The combined organic layers were passed through a phase separator and concentrated in vacuo. The resultant residue was purified by FCC, using 0-22% [2M NH3 in MeOH] in DCM to afford the title compound (425 mg, 57%). LCMS (Method 3): Rt 0.37/0.41 min, m/z 462. [MH+]. c. l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(l,4-dimethyl- 1 ,4,9-triaza-spir o [5.5] undec-9-yl)- [ 1 ,2,4] triazolo [4,3-a] pyridin-6-yloxy] - 1 ,2,3,4- tetrahydro-naphthalen-l-yl}-urea hydrochloride salt (Example 39)
A mixture of (5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-carbamic acid 2,2,2- trichloro-ethyl ester (US2004192653) (325 mg, 0.99 mmol), Intermediate 39b (420 mg, 0.90 mmol) and DIPEA ( 261 μί, 1.50 mmol) in 2-methyl-THF (4 mL) was stirred at 60 °C for 16.5 h and at 65°C for 3 h. The volatiles were concentrated in vacuo and the resultant residue was purified by FCC, using 0-20%> [2M NH3 in MeOH] in DCM to give a pale orange foam (403 mg, 70%). This was purified by MDAP to afford the title compound, monoformic acid salt (239 mg, 38%) as a white solid. This was dissolved in MeCN and water, treated with 1M HC1 (0.194μί, 0.194 mmol) and freeze-dried to afford the title compound, monohydrogen chloride salt (121 mg, 20%), as a white solid. LCMS (Method 5): Rt 2.86 min, m/z 641.4[MH+] . Ή NMR (400 MHz, DMSO): 8.53 (1H, s), 7.75 (1H, s), 7.66 (1H, d, J 9.62 Hz), 7.45-7.34 (3H, m), 7.34-7.22 (2H, m), 7.05 (1H, d, J 8.55 Hz), 6.03 (1H, s), 5.62- 5.56 (1H, m), 4.92-4.83 (1H, m), 3.59 (3H, s), 3.48-3.09 (10H, m, obscured by water), 2.93-2.64 (4H, m), 2.47-1.84 (10H, m), 1.20 (9H, s).
Biological assays
P38alpha enzyme inhibition assay
The inhibitory activity of compounds was determined using an Alphascreen® (Perkin Elmer) based kinase activity assay. Kinase reactions consisted of 25mM HEPES pH 7.5, lOmM MgCl2, ΙΟΟμΜ Na3V04, 2mM DTT, 0.05mg/ml Tween 20, ΙΟΟρΜ p38alpha (Invitrogen, PV3304), 1% DMSO and O^g/ml ATF-2 fusion protein (New England Biolabs, 9224). Compounds were incubated under these conditions for 2 hours, at 25°C, prior to the initiation of the kinase activity by the addition of the 250μΜ ATP. Reaction volumes were 20uL. After lhr at 25°C reactions were stopped by the adding lOuL of 25mM HEPES pH 7.5 containing 62.5mM EDTA, 0.05% Triton X-100, 10% BSA and 0.83ng/uL anti-phospho-ATF2 antibody (Abeam, ab28812). Detection was performed by measuring luminescence following the addition of Alphascreen Donor beads (Perkin Elmer 6765300) and Protein A Alphascreen Acceptor beads (Perkin Elmer 6760137), both at a final concentration of 20ug/ml. IC50 values were determined from concentration- response curves.
The compounds of the invention being exemplified and tested show p38a binding potencies (IC50 values) lower than ΙΟηΜ.
LPS-stimulated PBMC TNF release assay
Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy human volunteer blood using a standard density gradient centrifugation technique. Citrated blood was placed onto Histopaque™ and centrifuged. The PBMCs were removed from the density gradient interface and washed in phosphate buffered saline (PBS). The PBMCs were suspended in RPMI 1640 medium (without serum), dispensed into a 96- well plate and incubated at 37°C for 3h in a humidified incubator. After incubation, the medium was replaced (with medium containing 1 % foetal bovine serum) and the plate incubated at 37°C, for lh, in the presence of test compound or the appropriate vehicle. LPS (lOng/ml), or an appropriate vehicle control, was then added to the cells and the plate returned to the incubator for 18h. Cell-free supernatants were removed and assayed for TNFa levels using an ELISA kit from R&D Systems.
A dose response curve to each test compound was performed and the effect of compound in each experiment was expressed as a percentage inhibition of the control TNFa release. Dose response curves were plotted and compound potency (IC50) was determined. Compounds were tested in at least three separate experiments.
The compounds of the invention being exemplified and tested show p38a potencies (IC50 values) lower than ΙΟηΜ.

Claims

1. A compound of formula lly acceptable salt thereof
Figure imgf000171_0001
(I)
wherein:
W is a heteroatom selected from N or O, wherein N is substituted with -H, Ci- C6 alkyl or C3-C5 cycloalkyl;
Y is selected in the group consisting of: a group -S(0)p- wherein p is 0, 1 or 2; a group -0(CR3R4)n-; a group -(CR5R6)„-; a group -NR7-; a group -OC(O)-; a group -OC(0)NH-; and a group -OC(0)0-;
n is 0, 1 , 2 or 3;
R3, R4, R5 and R6 are each independently -H, fluorine or Ci-C6 alkyl, or, respectively, R3 and R4, or R5 and R6 may form together with the carbon atom to which they are attached a saturated 3-6 membered carbocyclic monocyclic ring optionally substituted by a group Ci-C6 alkyl, hydroxyl or halo;
R7 is -H, Ci-C6 alkyl, or C3-C7 cycloalkyl wherein such Ci-C6 alkyl or C3-C7 cycloalkyl are optionally substituted by a group C1 -C3 alkyl, C3-C6 cycloalkyl, hydroxyl, -CN or halo;
R1 is a group (Ila)
Figure imgf000171_0002
X2, X3, X4 and Xs are each independently a carbon atom, a nitrogen atom, a group -(CH)- or a group -NH-; such that each combination thereof forms an aromatic ring system;
R11 is linked to X4 and is selected from a group consisting of:
-NRARB, -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)- NRARB, -(Ci-C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C2- C6alkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)-NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)- (C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)- (C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3- C7cycloalkylene)-ORD, -N(Rc)C(0)N(RARB), -C(0)N(RARB), -N(Rc)C(0)N(Rc)- (C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C2- C6alkylene)-ORD, -0-(C3-C7cycloalkylene)-ORD, -S-(C2-C6alkylene)-ORD, -S-(C3- C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-ORD, -S(0)2N(Rc)-(C3- C7cycloalkylene)-ORD, -N(Rc)S(0)2-(C2-C6alkylene)-ORD, -N(Rc)S(0)2(C3- C7cycloalkylene)-ORD, -S(0)2N(RARB), -N(Rc)S(0)2RD, -N(Rc)C(0)Rc, ORc, SRC, -(C3-C7heterocycloalkyl), (C5-C7heterocycloalkyl)-(Ci-C6alkyl), (C5-
C7heterocycloalkyl)(C3-C6cycloalkyl) and (C3-C7 heterocycloalkyl)carbonyl, wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)- -(C2- C6alkylene)-, -(C3-C7cycloalkylene)-, -(C3-C7heterocycloalkyl) and (C5- C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxy 1 and halo; or
R11 is linked to X4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is optionally substituted by one or more group(s) independently selected from: halo, ORD, -CN, -S02RD, Ci-C6 alkyl which is substituted by one or more groups -CN; C3-C6 cycloalkyl which is substituted by a group selected from: -CN, -ORc, -SRC or halo ; -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-NRARB, -(Ci- C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)- NRARB, -S-(C2-C6alkylene)-NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)- (Ci-C6alkylene)-NRARB, -N(Rc)C(0)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)- (C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)- (C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)C(0)N(Rc)- (C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C3- C7cycloalkylene)-ORD, -S-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)- NRARB, -N(Rc)S(0)2-(C3-C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)- NRARB, -S(0)2N(Rc)-(C3-C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)- ORD, -S(0)2N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(C2-C6alkylene)-ORD, -N(Rc)S(0)2-(C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2RD, -(C3-C7heterocycloalkyl), (C5-C7heterocycloalkyl)-(Ci_C6alkyl), (C5-C7heterocycloalkyl)(C3-C6cycloalkyl) and (C3-C7heterocycloalkyl)carbonyl, wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)- -(C2-Cealkylene)-, -(C3-C7cycloalkylene)-, -(C3-
C7heterocycloalkyl) and -(C5-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the group consisting of: Ci-C6alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7cycloalkyl, hydroxyl, -CN and halo;
RA and RB are at each occurrence independently -H, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7 cycloalkyl, -ORD, -CN, -NRA RB ' or halo; alternatively, RA and RB, may form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more groups independently selected from -ORD, -CN, -NRA RB ', halo, Ci-C6 alkyl, -(C3-C7) cycloalkyl-NRA RB', -(Ci-C5 alkylene)-NRA'RB', -0-(Ci-C5 alkylene)-NRA RB ' and -0-(C3-C7cycloalkyl)- NRA'RB',-N(Rc')C(0)-(Ci-C6alkylene)-NRA'RB', -N(Rc')C(0)-(C3-C7cycloalkylene)-
B
NRA R1 -C(0)N(Rc')-(C2-C6alkylene)-NRA'RB', -C(0)N(Rc')-(C3-
C7cycloalkylene)-NRA'RB' such Ci-C6 alkyl, C2-C5 alkyl and C3-C7 cycloalkyl portions being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD, -CN, -NRA RB or halo; and which 6- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3- C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD, -CN, or halo; or RA and RB may be linked to one carbon atom of the -(Ci-Cealkylene)- or -(C3- C7cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
Rc is at each occurrence independently -H, Ci -C6 alkyl, (Ci-C4)hydroxy alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl, (Ci-C4)hydroxyalkyl and C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORD, -CN or halo;
alternatively Rc may be linked to one carbon atom of the -(C2-Cealkylene)- or -(C3- C7cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
RD is at each occurrence independently -H, -CH3, -C2H5 or (Ci-C4)hydroxyalkyl;
RA and RB are at each occurrence independently -H, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7 cycloalkyl, -ORD , -CN or halo; alternatively, RA and RB , may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -ORD, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD , -CN or halo; and which 6- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD', -CN, or halo; or RA' and RB ' may be linked to one carbon atom of the -(Ci- C6alkylene)- or -(C3-Cycycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
RD is at each occurrence independently -H, -CH3 or -C2H5;
Rc is at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl and C3-C6 cycloalkyl being optionally substituted by a group C1-C3 alkyl, -ORD', -CN or halo;
R13 are independently -H, Ci-C6 alkyl or halo;
A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms; said cycloalkylene ring being attached to W and Y, and optionally fused to a phenyl ring or to a monocyclic heteroaryl ring having 5 or 6 ring atoms, such phenyl or heteroaryl ring being optionally substituted by one or two groups R24;
R24 is at each occurrence independently selected from the group consisting of: Ci-Ce alkyl, halo and CN;
R2 is a radical of formula (Ilia)
R17\ N 2 1
1 1ε
R (Ilia)
R is a group of general formula (IV):
Figure imgf000175_0001
R19 is selected in a group consisting of: -H, -CH3, CN and OH; R20 is selected in the group consisting of: -H, -F, -CH3, -C2H5, -CH2OH, -CH2OMe, -CF2CF3, -CH2SCH3, -SCH3 and -SC2H5; R21 is -H, -CH3 or -C2H5; R18 is absent or is selected in the group consisting of aryl, heteroaryl, -(Ci-C6alkyl), -(C3-C7cycloalkyl), -(C3-C7heterocycloalkyl), (C5-Cvheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C6alkyl), -(C3-Cycycloalkyl), -(C3-Cyheterocycloalkyl), (C5- Cyheterocycloalkyl)-(Ci-C6alkyl) and (C5-Cyheterocycloalkyl)-(C3-C6 cycloalkyl) may be optionally substituted by one or more group(s) selected from -CN, -OH, =0, halo, -COORM, Ci-C6alkyl, C3-C6cycloalkyl, -0-(Ci-C6alkyl), -0-(C3-C6cycloalkyl), -S02RM; -S-(Ci-C6alkyl), -S-(C3-C6cycloalkyl), -NRHRJ, -N(RL)(C2-C6alkylene)- NRHRJ, -N(RL)(C3-C7cycloalkylene)-NRHRJ, -(Ci-C6alkylene)-NRHRJ, -(C3- C7cycloalkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ, -0-(C3-C7cycloalkylene)- NRHRJ, -S-(C2-C6alkylene)-NRHRJ, -S-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)- (Ci-C6alkylene)-NRHRJ, -N(RL)C(0)-(C3-C7cycloalkylene)-NRHRJ, -C(0)N(RL)- (C2-C6alkylene)-NRHRJ, -C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)N(RHRJ), -C(0)N(RHRJ), -N(RL)C(0)N(RL)-(C2-C6alkylene)-NRHRJ, -N(RL)C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)S(0)2-(Ci-C6alkylene)- NRHRJ, -N(RL)S(0)2-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RL)-(C2-C6alkylene)- NRHRJ, -S(0)2N(RL)-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RHRJ), ORL, SRL wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)-, -(C2- C6alkylene)-, -(C3-C7cycloalkylene)-, -(C3-C7heterocycloalkyl) and (C5- C7heterocycloalkyl) portion in the above listed groups, may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo;
RH and RJ, are at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group C1 -C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, -CN, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1 -C3 alkyl, C3-Cycycloalkyl, -ORM, -CN or halo; and which 6-1 1- membered saturated monocyclic or bicyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, - ORM, -CN, or halo;
RL is at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group C1 -C3 alkyl, -ORM, -CN or halo;
RM is at each occurrence independently -H, Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group hydroxyl, -CN or halo;
R° and RP are each independently Ci-C6 alkyl or Ci-C6 cycloalkyl, optionally substituted by a group C1 -C3 alkyl, -ORQ, -CN or halo; alternatively, R° and RP may form together with the nitrogen atom to which they are attached a 4-8- membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORQ, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, and which 6-8- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl;
RQ is -H, -CH3 or -C2H5;
z1, z2, z3, and z4 are independently selected in the group consisting of: C, N, S, O, a group -CH-, and a group -NH-, in such a combination that the resulting ring formed is an aromatic system;
with the proviso that when there is any combination among any of the substituents of R19 selected in a group consisting of -CH3 and R20 selected in the group consisting of -F, -CH3, -C2H5, -CH2OH, -CH2OMe, -CF2CF3, -CH2SCH3, -SCH3 and -SC2H5 and R21 selected from -CH3 and -C2H5; and
A is a divalent cycloalkylene radical having 5, 6 or 7 ring atoms fused to a phenyl ring or monocyclic heteroaryl,
then
R11 is -NRARB, -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-
NRARB, -(Ci-C6alkylene)-NRARB, -(C3-C7cycloalkylene)-NRARB, -0-(C2- C6alkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)-NRARB, - S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)- (C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)- (C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)N(RARB), -C(0)N(RARB),
-N(Rc)C(0)N(Rc)-(C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3- C7cycloalkylene)-NRARB, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB, -S(0)2N(RARB), wherein any of such -(Ci-C6alkylene)- -(C2-C6alkylene)-, -(C3-C7cycloalkylene)- and -(C3-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the list consisting of: Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein
RA and RB are at each occurrence independently form together with the nitrogen atom to which they are attached a azetidine or a 4- 1 1- membered saturated heterocyclic monocyclic or bicyclic ring system which is substituted by -NRA RB , -(Ci-C5alkylene)-NRA'RB', -0-(Ci-C5alkyl)-NRA RB', -0-(C3-C7cycloalkyl)- NRA RB , -N(Rc')C(0)-(Ci-C6alkylene)-NRA RB', -N(Rc')C(0)-(C3-
C7cycloalkylene)-NRA'RB', -C(0)N(Rc')-(C2-C6alkylene)-NRA'RB', -C(0)N(Rc')- (C3-C7cycloalkylene)-NRA RB and optionally by one or more group selected from -ORD, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD, -CN, -NRA RB or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3- C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD, -CN, or halo; or R11 is linked to X4 and is phenyl or 5- or 6-membered monocyclic heteroaryl, wherein such phenyl or 5- or 6-membered monocyclic heteroaryl is substituted by two or more group(s) selected from: -N(RC)(C2-C6alkylene)-NRARB, -N(Rc)(C3-C7cycloalkylene)-NRARB, -(Ci-C6alkylene)-NRARB, -(C3-
C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)-NRARB, -S-(C2-C6alkylene)- NRARB, -S-(C3-C7cycloalkylene)-NRARB, -N(Rc)C(0)-(Ci-C6alkylene)-NRARB, -N(Rc)C(0)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-NRARB, -C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -C(0)N(Rc)-(C2-C6alkylene)-ORD, -C(0)N(Rc)-(C3-C7cycloalkylene)-ORD, -N(Rc)C(0)N(Rc)-(C2-C6alkylene)-NRARB, -N(Rc)C(0)N(Rc)-(C3-C7cycloalkylene)-NRARB, -0-(C3-C7cycloalkylene)-ORD, -S- (C3-C7cycloalkylene)-ORD, -N(Rc)S(0)2-(Ci-C6alkylene)-NRARB, -N(Rc)S(0)2-(C3- C7cycloalkylene)-NRARB, -S(0)2N(Rc)-(C2-C6alkylene)-NRARB, -S(0)2N(Rc)-(C3- C7cycloalkylene)-NRARB; and additionally optionally substituted by one or more group(s) selected from: Ci-C6 alkyl, C3-C6 cycloalkyl, halo, -ORD, -CN, S02RD, -CONRARB wherein any of such Ci-C6alkyl, C3-C6cycloalkyl, -(Ci-Cealkylene)-, -(C2-C6alkylene)-, -(C3-C7cycloalkylene)- and -(C3-C7heterocycloalkyl) portion in the above listed groups may be optionally substituted by one, two or three groups R25 which are independently selected in the group consisting of: Ci-C6alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7cycloalkyl, hydroxyl, CN and halo;
wherein RA and RB are at each occurrence independently hydrogen, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD', -CN, -NRA RB' or halo; alternatively, RA and RB, may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic or bicyclic ring system which is optionally substituted by one or more group -ORD , -CN, -NRA RB , halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1-C3 alkyl, C3-C7cycloalkyl, -ORD , -CN or halo; and which 6-1 1- membered saturated heterocyclic monocyclic or bicyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORD , -CN, or halo; and/or RA and RB may be linked to one carbon atom of the -(Ci-Cealkylene)-, -(C2-C6alkylene)- or -(C3-C7Cycloalkylene)- portion of the group linked to the nitrogen to which they are connected to form a saturated cycle of up to 6 ring atoms;
or
- R18 is selected in the group consisting of aryl, heteroaryl, -(Ci-C6alkyl), -(C3-C7cycloalkyl), -(C3-C7heterocycloalkyl), (C5-C7heterocycloalkyl)-(Ci-C6alkyl) and (C5-C7heterocycloalkyl)-(C3-C6 cycloalkyl), wherein any of such aryl, heteroaryl, -(Ci-C6alkyl), -(C3-C7cycloalkyl), -(C3-C7heterocycloalkyl), (C5- C7heterocycloalkyl)-(Ci-C6alkyl) and (C5-C7heterocycloalkyl)-(C3-C6 cycloalkyl) is substituted by a group selected from NRHRJ, -N(RL)(C2-C6alkylene)-NRHRJ, -N(RL)(C3-C7cycloalkylene)-NRHRJ, -(Ci-C6alkylene)-NRHRJ, -(C3- C7cycloalkylene)-NRHRJ, -0-(C2-C6alkylene)-NRHRJ, -0-(C3-C7cycloalkylene)- NRHRJ, -S-(C2-C6alkylene)-NRHRJ, -S-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)- (Ci-C6alkylene)-NRHRJ, -N(RL)C(0)-(C3-C7cycloalkylene)-NRHRJ, -C(0)N(RL)- (C2-C6alkylene)-NRHRJ, -C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)C(0)N(RHRJ), -C(0)N(RHRJ), -N(RL)C(0)N(RL)-(C2-C6alkylene)-NRHRJ, -N(RL)C(0)N(RL)-(C3-C7cycloalkylene)-NRHRJ, -N(RL)S(0)2-(Ci-C6alkylene)- NRHRJ, -N(RL)S(0)2-(C3-C7cycloalkylene)-NRHRJ, -S(0)2N(RL)-(C2-C6alkylene)- NRHRJ, -S(0)2N(RL)-(C3-C7cycloalkylene)-NRHRJ, and wherein any portion in the above listed groups may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo; and wherein RH and RJ at each occurrence independently form together with the nitrogen atom to which they are attached an azetidine or a 4- 1 1 membered saturated monocyclic or bicyclic heterocyclic ring system which is substituted by NR°RP and optionally by one or more additional groups -ORM, -CN, halo, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3-C7 cycloalkyl being optionally substituted by a group C1-C3 alkyl, C3-C7cycloalkyl, -ORM, CN or halo; and which 6-1 1- membered saturated monocyclic or bicyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl, wherein any of such Ci-C6 alkyl or C3-C6 cycloalkyl may be optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -ORM, CN, or halo;
wherein RA', RB', Rc, Rc', RD, RD', RH, RJ, RL, RM, R° and RP are as defined above.
2. A compound of formula (IA) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4 - and n is 0, A is group
Figure imgf000181_0001
wherein R is a group of formula (Ilaa)
Figure imgf000181_0002
and wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group
Figure imgf000181_0003
* wherein R25 represents one, two or three substituents independently selected in the list consisting of Ci-C6 alkyl, (C1 -C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein R2 is a radical of formula (Ilia)
Figure imgf000182_0001
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000182_0002
wherein R 19 , R2z0u and R 2Z11 are 18
-CH3 and wherein R is phenyl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -O- (C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, -CN or halo.
3. A compound of formula (IB) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0 A is group
Figure imgf000182_0003
or
wherein R1 is a group of formula (Ilaa)
Figure imgf000182_0004
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group -NRARB, wherein RA and RB form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(Ci-C5alkyl)-NRA RB', wherein RA' and RB' are independently -H or Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, C3-Cycycloalkyl, -ORD , -CN or halo; or R is a group
Figure imgf000183_0001
wherein R25 represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (C1 -C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1 z
' 18
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000183_0002
wherein R 19 , R2z0u and R 2Z11 are -CH3 and wherein 18
R is heteroaryl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -O- (C2-C6alkylene)-NRHRJ, wherein RH and RJ form together with the nitrogen atom to which they are attached an azetidine or a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, -CN, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl, such Ci-C6 alkyl and C3- C7 cycloalkyl being optionally substituted by a group C1 -C3 alkyl, C3-Cycycloalkyl, wherein R° and RP are each independently Ci-C6 alkyl.
4. A compound of formula (IC) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000184_0001
R is a group:
Figure imgf000184_0002
wherein R is optionally present and represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; R2 is a radical of formula (Ilia)
Figure imgf000184_0003
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000184_0004
19 20 21 18
wherein R , Rzu and RZ1 are H and R is phenyl optionally substituted by -O- (Ci-C6alkylene)-NRHRJ, wherein RH and RJ are Ci-C6 alkyl.
5. A compound of formula (ID) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is group
Figure imgf000184_0005
R1 is a group of formula (Ilaa) 11
Figure imgf000185_0001
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group
Figure imgf000185_0002
wherein R25 is optionally present and represents one two or three substituents independently selected in the list consisting of Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1
r1 B (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000185_0003
19 20 21 18
wherein Riy is -H, Rzu and RZ1 are -CH3 and R is heteroaryl substituted by a -(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1-C3 alkyl, -ORM, -CN or halo; alternatively, RH and RJ may form together with the nitrogen atom to which they are attached a 4-1 1 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups -ORM, halo, NR°RP, Ci-C6 alkyl or C3-C7 cycloalkyl; and which 6-1 1- membered saturated monocyclic heterocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl optionally substituted by a group Ci-C6 alkyl, C3-C7 cycloalkyl, -CN or halo.
6. A compound of formula (IE) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is
Figure imgf000186_0001
R1 is a group of formula (Ilaa)
Figure imgf000186_0002
R * (Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and X2 is a group -CH-, and R13 is -H; wherein R11 is a group
Figure imgf000186_0003
wherein R25 is optionally present and represents one two or three substituents independently selected in the list consisting of Ci-C6 alkyl, (C1-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1
' 18
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
(IV)
Figure imgf000187_0001
19 20 21 18
wherein R and R are H and R is -CH3 and R is heteroaryl substituted by a -(Ci-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl optionally substituted by a group C1 -C3 alkyl, -CN or halo.
7. A compound of formula (IF) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0, A is grou
Figure imgf000187_0002
wherein R is a group of formula (Ilaa)
1 1
Figure imgf000187_0003
R (Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is a group -NRARB, wherein RA and RB form together with the nitrogen atom to which they are attached a 4-1 1- membered saturated heterocyclic monocyclic ring system which is substituted by -(Ci-C5alkyl)-NRA RB', wherein RA' and RB' are independently -H or Ci-C6 alkyl optionally substituted by a group Ci-C3 alkyl, C3-C7cycloalkyl, -ORD', -CN or halo; wherein R2 is a radical of formula (Ilia)
R17\ N 2 1 z
' 18
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
(IV)
Figure imgf000188_0001
19 20 21 18
wherein R , Rzu and RZ 1 are -CH3 and wherein R is -Ci-C6alkyl, optionally substituted by Ci-C6 alkyl, C3-C7 cycloalkyl, -ORL or halo.
8. A compound of formula (IG) according to claim 1 , wherein W is NH, Y is a
. 3π 4
group -0(CR R )n- and n is 0, A is grou
Figure imgf000188_0002
wherein R is a group of formula (Ilaa) which is connected to the group Y through the carbon adjacent to X
Figure imgf000188_0003
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is phenyl which is optionally substituted by one or more group(s) selected from halo, Ci-C6 alkyl, wherein such Ci-C6 alkyl is optionally substituted by -(Ci-Cealkylene)-NRARB, wherein RA and RB may form together with the nitrogen atom to which they are attached a 4- 1 1 - membered saturated heterocyclic monocyclic ring system which is optionally substituted by one or more groups selected from -ORD, -CN, halo, Ci-C6 alkyl or C3- C7 cycloalkyl; and which 6- 1 1 - membered saturated heterocyclic monocyclic ring optionally contains a further heteroatom which is oxygen or nitrogen, said nitrogen atom optionally substituted by Ci-C6 alkyl or C3-C6 cycloalkyl; wherein R2 is a radical of formula (Ilia)
Figure imgf000189_0001
1 2 3 4 18 17
wherein z =-CH-, z = C, z is O, z is N, R is absent, and R is a group general formula (IV)
Figure imgf000189_0002
wherein R19, R20 and R21 are -CH3 and R18 is -(Ci-C6alkyl).
9. A compound of formula (IH) according to claim 1 , wherein W is NH, Y is a
> 3r> 4
group -0(CR R )n- and n is 0, A is
Figure imgf000189_0003
wherein R1 is a group of formula (Ilaa)
Figure imgf000189_0004
(Ilaa)
wherein X4 is a carbon atom, X5 is a nitrogen atom, X3 is a nitrogen atom and
X2 is a group -CH-, and R13 is -H; wherein R11 is a group
Figure imgf000189_0005
wherein R25 represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci-C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; wherein R2 is a radical of formula (Ilia)
Figure imgf000190_0001
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000190_0002
19 20 21 18
wherein R , Rzu and RZ 1 are -CH3 and wherein R is aryl or heteroaryl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -0-(C2-C6alkylene)-NRHRJ, wherein RH and RJ are independently Ci-C6 alkyl or C3-C6 cycloalkyl, such Ci-C6 alkyl or C3-C6 cycloalkyl being optionally substituted by a group Ci-C3 alkyl, -ORM, -CN or halo.
10. A compound of formula (IL) according to claim 1 , wherein W is NH, Y is a group -0(CR3R4)n- and n is 0 A is group
Figure imgf000190_0003
or
R is a group:
Figure imgf000190_0004
wherein R25 is optionally present and represents one or two substituents independently selected in the list consisting of Ci-C6 alkyl, (Ci-C3) haloalkyl, (Ci- C4)hydroxyalkyl, C3-C7 cycloalkyl, hydroxyl and halo; R2 is a radical of formula (Ilia)
Figure imgf000191_0001
R (Ilia)
wherein z1=-CH-, z2=C, z3 and z4 are N and R17 is a group of general formula
Figure imgf000191_0002
19 20 21
wherein R , Rzu and RZ1 are CH3 and R 18 is phenyl optionally substituted by a group selected from Ci-C6 alkyl, -(C2-Cealkylene)-NRHRJ and -0-(C2-Cealkylene)- NRHRJ, wherein RH and RJ are Ci-C6 alkyl or RH and RJ form together with the nitrogen atom to which they are attached an azetidine or a 4-11 membered saturated monocyclic heterocyclic ring system which is optionally substituted by one or more groups halo or NR°RP, wherein R° and RP are each independently Ci-C6 alkyl.
11. A compound according to any one of the preceding claims selected from:
• l-[ -(2-Dimethylamino-ethyl)-3-isopropyl- H-[l,4']bipyrazolyl-5-yl]-3- {(lS,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-{3-isopropyl- -[2-(4- methyl-piperazin- 1 -yl)-ethyl]- 1 Ή-[ 1 ,4']bipyrazolyl-5-yl} -urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[3-isopropyl-l'-(2- pyrrolidin-l-yl-ethyl)- H-[l,4']bipyrazolyl-5-yl]-urea formate salt;
l-{(lS,4R)-4-[3-((2S,6R)-2,6-Dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3- a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-3-[3-isopropyl-l'-(2- [l,4]oxazepan-4-yl-ethyl)-l'H-[l,4']bipyrazolyl-5-yl]-urea formate salt;
• 1 - [ 1 '-(2-Dimethylamino-ethyl)-3 -isopropyl- 1 Ή- [ 1 ,3 ']bipyrazolyl-5 -yl] -3 - {(lS,4R)-4 3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3a]pyridin-6- yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
• l-[ -(2-Dimethylamino-ethyl)-3-isopropyl- H-[l,4']bipyrazolyl-5-yl]-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
l-[l*-(2-Dimethylamino-ethyl)-3-ethyl-l*H-[l,4,]bipyrazolyl-5-yl]-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1,2,3,4-tetrahydro-naphthalen-l-yl}- urea formate salt;
l-[l*-(2-Dimethylamino-ethyl)-3-ethyl-l*H-[l,4,]bipyrazolyl-5-yl]-3- {(lS,4R)-4 3-((2S,6R)-2,6-dimethyl-piperidin-l-yl) 1,2,4]triazolo[4,3a]pyridin-^ yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
• l-{2-[3-(2-Dimethylamino-ethoxy)-phenyl]-5-methyl-2H-pyrazol-3-yl}-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
· 1 - [ 1 *-(2- Azetidin- 1 -yl-ethyl)-3 -tert-butyl- 1 Ή- [ 1 ,4*]bipyrazolyl-5 -yl] -3 -
{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]- l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
1 - {3-tert-Butyl- 1 '-[2-(3-dimethylamino-azetidin- 1 -yl)-ethyl]- 1 Ή-
[l,4']bipyrazolyl-5-yl}-3-{(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)- [l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro-naphthalen-l-yl}-urea formate salt;
• l-{5-tert-Butyl-2-[3-(3-dimethylamino-azetidin-l-ylmethyl)-phenyl]-2H- pyrazol-3-yl}-3- {(lS,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3- a]pyridin-6-yloxy]- 1 ,2,3 ,4-tetrahydro-naphthalen- 1 -yl} -urea formate salt;
· l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((lS,4R)-4-{3-[2,6-dichloro-4- (4-methyl-piperazin-l-ylmethyl)-phenyl]-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy}- l,2,3,4-tetrahydro-naphthalen-l-yl)-urea formate salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperi
tetrahydro-naphthalen-l-yl}-urea formate salt;
l-(5-tert-Butyl-isoxazol-3-yl)-3- {(l S,4R)-4-[3-(4-dimethylaminomethyl- piperidin-l-yl)-[ 1 ,2, 4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3, 4-tetrahydro-naphthalen- l-yl}-urea;
l-(3-tert-Butyl-l ,-methyl-l 'H-[l ,4,]bipyrazolyl-5-yl)-3- {(l S,4R)-4-[3-(4- dimethylaminomethyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l ,2,^ tetrahydro-naphthalen-l-yl}-urea formate salt;
l-[3-tert-Butyl-l '-(2-dimethylamino-ethyl)-l'H-[l ,4']bipyrazolyl-5-yl]-3- {4- [3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl} -urea;
l-(5-tert-Butyl)-2-p-tolyl-2H-pyrazol-3-yl)-3- {4-[3-((S)-2-methyl-piperidin- l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-cyclohexyl}-urea;
• l- {5-tert-Butyl-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H-pyrazol-3-yl}-3- {4-[3-((S)-2-methyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- cyclohexyl} -urea;
• l- {5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3- {(l S,4R)-4-[3-((S)-2-methyl-piperidin-l-yl)-[l ,2,4]triazolo[4,3- a]pyridin-6-yloxy]- 1 ,2,3 ,4-tetrahydronaphthalen- 1 -yl} -urea;
· l- {5-(Cyano-dimethyl-methyl)-2-[3-(2-dimethylamino-ethoxy)-phenyl]-2H- pyrazol-3-yl}-3- {(l S,4R)-4-[3-((2S,6R)-2,6-dimethyl-piperidin-l-yl)- [l ,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l ,2,3,4-tetrahydro-naphthalen-l-yl} -urea;
1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(2-dimethylamino- ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrafiydro- naphthalen-l-yl)-urea hydrogen chloride salt;
l-[5-tert-Butyl-2-(2-hydroxy-ethyl)-2H-pyrazol-3-yl]-3-((lS,4R)-4-{3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt; l-(3-tert-Butyl '-methyl H-[l,4']bipyrazolyl-5-yl)-3-((lS,4R)-4-{3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt;
l-(5-tert-Butyl-isoxazol-3-yl)-3-((lS,4R)-4-{3-[4-(2-dimethylamino-ethoxy)- piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrahydro-naphthalen- 1 - yl)-urea hydrogen chloride salt;
1 -(5-tert-Butyl-2-propyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(2-dimethylamino- ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4-tetrahydro- naphthalen- l-yl)-urea hydrogen chloride salt;
· 1 -[5-tert-Butyl-2-(2-methoxy-ethyl)-2H-pyrazol-3-yl]-3-((l S,4R)-4- {3-[4-(2- dimethylamino-ethoxy)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen- l-yl)-urea hydrogen chloride salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((S)-3-{[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1,2,3 ,4-tetrahydro-naphthalen- 1 -yl} -urea hydrogen chloride salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((S)-3- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen- 1-yl} -urea hydrogen chloride salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(4- {[(2-methoxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3 ,4-tetrahydro-naphthalen- 1 -yl} -urea;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(4- {[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1, 2,3, 4-tetrahydro-naphthalen- 1-yl} -urea hydrogen chloride salt;
· l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4-[l,4]oxazepan-4- ylmethyl-piperidin-l-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen- 1-yl} -urea hydrogen chloride salt;
1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-((l S,4R)-4- {3-[4-(4-methyl- piperazin- 1 -ylmethyl)-piperidin- 1 -yl]-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy} - 1 ,2,3,4- tetrahydro-naphthalen-l-yl)-urea hydrogen chloride salt;
l-(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-((R)-3-{[(2-hydroxy- ethyl)-methyl-amino]-methyl} -piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1,2,3 ,4-tetrahydro-naphthalen- 1 -yl} -urea hydrogen chloride salt;
l-[5-tert-Butyl-2-(2-methoxy-ethyl)-2H-pyrazol-3-yl]-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen- 1 -yl} -urea;
l-(5-tert-Butyl-2-ethyl-2H-pyrazol-3-yl)-3-{(lS,4R)-4-[3-(4- dimethylaminomethyl-piperidin- 1 -yl)-[ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4- tetrahydro-naphthalen-l-yl}-urea formate salt;
N- [5 -tert-Butyl-3 -(3 -{(IS ,4R)-4- [3 -(4-dimethylaminomethyl-piperidin- 1 -yl)- [ 1 ,2,4]triazolo[4,3-a]pyridin-6-yloxy]- 1 ,2,3,4-tetrahydro-naphthalen- 1 -yl} -ureido)-2- methoxyphenyljmethanesulfonamide hydrogen chloride salt;
· 1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-((2S,4S)-4- dimethylamino-l-methyl-pyrrolidin-2-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4- tetrahydro-naphthalen-l-yl}-urea hydrochloride salt;
1 -(5-tert-Butyl-2-methyl-2H-pyrazol-3-yl)-3- {(1 S,4R)-4-[3-(l ,4-dimethyl- 1 ,4,9- triaza-spiro[5.5]undec-9-yl)-[l,2,4]triazolo[4,3-a]pyridin-6-yloxy]-l,2,3,4-tetrahydro- naphthalen-l-yl}-urea hydrochloride salt
and pharmaceutically acceptable salts thereof.
12. A pharmaceutical composition comprising a compound as claimed in any of the preceding claims 1 to 1 1 , together with one or more pharmaceutically acceptable carriers.
13. A compound as claimed in any of claims 1 to 1 1 for use in the treatment of diseases or conditions which benefit from inhibition of p38 MAP kinase activity.
14. A compound according to any one of claims 1 to 1 1 for use in the treatment of diseases or conditions which benefit from inhibition of p38 MAP kinase activity according to claim 13 wherein the diseases or conditions is chronic eosinophilic pneumonia, asthma, COPD, adult respiratory distress syndrome (ARDS), exacerbation of airways hyper-reactivity consequent to other drug therapy or airways disease that is associated with pulmonary hypertension.
15. A method of treating diseases or conditions in a human subject which benefit from inhibition of p38 MAP kinase activity comprising administering to such subject in need thereof a compound as claimed in any of claims 1 to 1 1.
16. A method according to claim 15, wherein the disease or condition is chronic eosinophilic pneumonia, asthma, COPD, adult respiratory distress syndrome (ARDS), exacerbation of airways hyper-reactivity consequent to other drug therapy or airways disease that is associated with pulmonary hypertension.
17. The use of a compound as claimed in any of claims 1 to 1 1 in the manufacture of a medicament for the treatment of diseases or conditions which benefit from inhibition of p38 MAP kinase activity.
18. The use according to claim 17 wherein the disease or condition is chronic eosinophilic pneumonia, asthma, COPD, adult respiratory distress syndrome (ARDS), exacerbation of airways hyper-reactivity consequent to other drug therapy or airways disease that is associated with pulmonary hypertension.
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WO2017108737A1 (en) 2015-12-23 2017-06-29 Chiesi Farmaceutici S.P.A. 1-(3-tert-butyl-phenyl)-3-(4-([1,2,4]triazolo[4,3-a]pyridin-6-yloxy)-1,2,3,4-tetrahydro- naphthalen-1-yl)-urea derivatives and their use as p38 mapk inhibitors
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US11291659B2 (en) 2017-10-05 2022-04-05 Fulcrum Therapeutics, Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD
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JP7459048B2 (en) 2019-03-04 2024-04-01 タツタ電線株式会社 conductive adhesive composition
US11427558B1 (en) 2019-07-11 2022-08-30 ESCAPE Bio, Inc. Indazoles and azaindazoles as LRRK2 inhibitors

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