WO2014056896A3 - One-step biomolecular immobilisation procedure and products thereof - Google Patents

One-step biomolecular immobilisation procedure and products thereof Download PDF

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Publication number
WO2014056896A3
WO2014056896A3 PCT/EP2013/070919 EP2013070919W WO2014056896A3 WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3 EP 2013070919 W EP2013070919 W EP 2013070919W WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3
Authority
WO
WIPO (PCT)
Prior art keywords
products
immobilisation
biomolecular
step
biomolecule
Prior art date
Application number
PCT/EP2013/070919
Other languages
French (fr)
Other versions
WO2014056896A2 (en
Inventor
Sandeep Kumar VASHIST
Günter Roth
Jürgen Burger
Gregor CZILWIK
Original Assignee
Albert-Ludwigs-Universität Freiburg
Hsg-Imit Institut Für Mikro- Und Informationstechnologie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP12187638 priority Critical
Priority to EP12187638.7 priority
Application filed by Albert-Ludwigs-Universität Freiburg, Hsg-Imit Institut Für Mikro- Und Informationstechnologie filed Critical Albert-Ludwigs-Universität Freiburg
Publication of WO2014056896A2 publication Critical patent/WO2014056896A2/en
Publication of WO2014056896A3 publication Critical patent/WO2014056896A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Abstract

The invention relates to a method for covalent immobilisation of a biomolecule to a solid material and products arising therefrom, comprising providing a solid material with an activated surface, preferably by treatment with an oxidising agent, and subsequently contacting said material with a mixture of biomolecule to be immobilised and one or more silane components, preferably present in a pre-mixed binding solution. The immobilisation procedure is particularly useful for immunoassays, enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance immunoassays, microarrays or microfluidic assays.
PCT/EP2013/070919 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof WO2014056896A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP12187638 2012-10-08
EP12187638.7 2012-10-08

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13773780.5A EP2903740A2 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Publications (2)

Publication Number Publication Date
WO2014056896A2 WO2014056896A2 (en) 2014-04-17
WO2014056896A3 true WO2014056896A3 (en) 2014-07-03

Family

ID=47010351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/070919 WO2014056896A2 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Country Status (2)

Country Link
EP (1) EP2903740A2 (en)
WO (1) WO2014056896A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105498706A (en) * 2015-12-09 2016-04-20 广东研捷医药科技有限公司 Preparation method for protein A immunoaffinity column

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DE102015003404A1 (en) * 2015-03-18 2016-09-22 Forschungszentrum Jülich GmbH A process for preparing a standard for the detection of protein aggregates of a proteopathy as well as standard and its use
CN104897758A (en) * 2015-06-26 2015-09-09 王利兵 Method for manufacturing and modifying electrochemiluminescence electrode array
EP3317666A1 (en) * 2015-06-30 2018-05-09 IMEC vzw Surface immobilization of an analyte-recognizing molecule
CN104965076B (en) * 2015-07-14 2017-01-18 上海拜豪生物科技有限公司 One kind cadmium chelating immune complexes and preparation method and application
US20180194914A1 (en) * 2015-07-14 2018-07-12 Nanosniff Technologies Pvt. Ltd. Process for immobilizing one or more receptor biomolecules on one or more solid surfaces
WO2017172879A1 (en) * 2016-03-29 2017-10-05 Oneighty°C Technologies Corporation Method for rapidly determining effective sterilization, demmunization, and/or disinfection
CN106335234B (en) * 2016-08-19 2018-10-19 中国科学院上海微系统与信息技术研究所 A graphene composite film and method for preparing a protein non-covalently modified based
CN106814116A (en) * 2017-02-20 2017-06-09 华南农业大学 Mark-free type acrylamide electrochemical immunosensor and building method and application thereof

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EP1365249A1 (en) * 2001-02-08 2003-11-26 Ngk Insulators, Ltd. Biochip and method of manufacturing the biochip
US20050079486A1 (en) * 2003-09-23 2005-04-14 Wiscnsin Alumni Research Foundation - Using liquid crystals to detect affinity microcontact printed biomolecules
WO2010044083A2 (en) * 2008-10-14 2010-04-22 Dublin City University A multi-well plate for biological assays

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IE20080934A1 (en) 2007-11-22 2009-09-02 Univ Dublin City A method of immobilising biological molecules to a support and products thereof

Patent Citations (3)

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EP1365249A1 (en) * 2001-02-08 2003-11-26 Ngk Insulators, Ltd. Biochip and method of manufacturing the biochip
US20050079486A1 (en) * 2003-09-23 2005-04-14 Wiscnsin Alumni Research Foundation - Using liquid crystals to detect affinity microcontact printed biomolecules
WO2010044083A2 (en) * 2008-10-14 2010-04-22 Dublin City University A multi-well plate for biological assays

Non-Patent Citations (9)

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Title
CHANDRA KUMAR DIXIT ET AL: "Development of a High Sensitivity Rapid Sandwich ELISA Procedure and Its Comparison with the Conventional Approach", ANALYTICAL CHEMISTRY, vol. 82, no. 16, 15 August 2010 (2010-08-15), pages 7049 - 7052, XP055048836, ISSN: 0003-2700, DOI: 10.1021/ac101339q *
DAN ZHENG ET AL: "Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene", TALANTA, vol. 99, 15 May 2012 (2012-05-15), pages 22 - 28, XP055093586, ISSN: 0039-9140, DOI: 10.1016/j.talanta.2012.05.014 *
DAN ZHENG ET AL: "Rapid and simple preparation of a reagentless glucose electrochemical biosensor", THE ANALYST, vol. 137, no. 16, 1 January 2012 (2012-01-01), pages 3800, XP055048865, ISSN: 0003-2654, DOI: 10.1039/c2an35128e *
DIXIT CHANDRA KUMAR ET AL: "Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 439 - 445, XP009166031, ISSN: 1750-2799 *
S K VASHIST ET AL: "Rapid Immunodiagnostic Kits based on proprietary 1-step chemistry for covalent and leach-proof antibody immobilisation", MIKROSYSTEMTECHNIK - KONGRESS 2013, AACHEN, GERMANY, 14-16 OCT. 2013; POSTER 1.13, 15 October 2013 (2013-10-15), http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/vashist-rapid-immunodiagnostic-kits, pages 384 - 387, XP055093601 *
SANDEEP KUMAR VASHIST ET AL: "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay", THE ANALYST, vol. 136, no. 21, 1 January 2011 (2011-01-01), pages 4431, XP055048842, ISSN: 0003-2654, DOI: 10.1039/c1an15325k *
SANDEEP KUMAR VASHIST: "A sub-picogram sensitive rapid chemiluminescent immunoassay for the detection of human fetuin A", BIOSENSORS AND BIOELECTRONICS, vol. 40, no. 1, 1 February 2013 (2013-02-01), pages 297 - 302, XP055093588, ISSN: 0956-5663, DOI: 10.1016/j.bios.2012.07.067 *
SANDEEP KUMAR VASHIST: "Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications", DIAGNOSTICS, vol. 2, no. 3, 27 August 2012 (2012-08-27), pages 23 - 33, XP055093581, DOI: 10.3390/diagnostics2030023 *
YANG LUO ET AL: "Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay", JOURNAL OF TRANSLATIONAL MEDICINE, BIOMED CENTRAL, LONDON, GB, vol. 10, no. 1, 6 February 2012 (2012-02-06), pages 24, XP021118747, ISSN: 1479-5876, DOI: 10.1186/1479-5876-10-24 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105498706A (en) * 2015-12-09 2016-04-20 广东研捷医药科技有限公司 Preparation method for protein A immunoaffinity column

Also Published As

Publication number Publication date
WO2014056896A2 (en) 2014-04-17
EP2903740A2 (en) 2015-08-12

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