WO2014056896A3 - One-step biomolecular immobilisation procedure and products thereof - Google Patents

One-step biomolecular immobilisation procedure and products thereof Download PDF

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Publication number
WO2014056896A3
WO2014056896A3 PCT/EP2013/070919 EP2013070919W WO2014056896A3 WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3 EP 2013070919 W EP2013070919 W EP 2013070919W WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3
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WO
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Patent type
Prior art keywords
products
immobilisation
biomolecular
step
biomolecule
Prior art date
Application number
PCT/EP2013/070919
Other languages
French (fr)
Other versions
WO2014056896A2 (en )
Inventor
Sandeep Kumar VASHIST
Günter Roth
Jürgen Burger
Gregor CZILWIK
Original Assignee
Albert-Ludwigs-Universität Freiburg
Hsg-Imit Institut Für Mikro- Und Informationstechnologie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Abstract

The invention relates to a method for covalent immobilisation of a biomolecule to a solid material and products arising therefrom, comprising providing a solid material with an activated surface, preferably by treatment with an oxidising agent, and subsequently contacting said material with a mixture of biomolecule to be immobilised and one or more silane components, preferably present in a pre-mixed binding solution. The immobilisation procedure is particularly useful for immunoassays, enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance immunoassays, microarrays or microfluidic assays.
PCT/EP2013/070919 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof WO2014056896A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP12187638 2012-10-08
EP12187638.7 2012-10-08

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20130773780 EP2903740A2 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Publications (2)

Publication Number Publication Date
WO2014056896A2 true WO2014056896A2 (en) 2014-04-17
WO2014056896A3 true true WO2014056896A3 (en) 2014-07-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/070919 WO2014056896A3 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Country Status (2)

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EP (1) EP2903740A2 (en)
WO (1) WO2014056896A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105498706A (en) * 2015-12-09 2016-04-20 广东研捷医药科技有限公司 Preparation method for protein A immunoaffinity column

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CN104897758A (en) * 2015-06-26 2015-09-09 王利兵 Method for manufacturing and modifying electrochemiluminescence electrode array
EP3317666A1 (en) * 2015-06-30 2018-05-09 IMEC vzw Surface immobilization of an analyte-recognizing molecule
US20180194914A1 (en) * 2015-07-14 2018-07-12 Nanosniff Technologies Pvt. Ltd. Process for immobilizing one or more receptor biomolecules on one or more solid surfaces
CN104965076B (en) * 2015-07-14 2017-01-18 上海拜豪生物科技有限公司 One kind cadmium chelating immune complexes and preparation method and application
WO2017172879A1 (en) * 2016-03-29 2017-10-05 Oneighty°C Technologies Corporation Method for rapidly determining effective sterilization, demmunization, and/or disinfection
CN106335234A (en) * 2016-08-19 2017-01-18 中国科学院上海微系统与信息技术研究所 Graphene and protein composite film based on non-covalent modification and preparation method thereof

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EP1365249A1 (en) * 2001-02-08 2003-11-26 Ngk Insulators, Ltd. Biochip and method of manufacturing the biochip
US20050079486A1 (en) * 2003-09-23 2005-04-14 Wiscnsin Alumni Research Foundation - Using liquid crystals to detect affinity microcontact printed biomolecules
WO2010044083A2 (en) * 2008-10-14 2010-04-22 Dublin City University A multi-well plate for biological assays

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WO2009066275A1 (en) 2007-11-22 2009-05-28 Dublin City University A method of immobilising biological molecules to a support and products thereof

Patent Citations (3)

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EP1365249A1 (en) * 2001-02-08 2003-11-26 Ngk Insulators, Ltd. Biochip and method of manufacturing the biochip
US20050079486A1 (en) * 2003-09-23 2005-04-14 Wiscnsin Alumni Research Foundation - Using liquid crystals to detect affinity microcontact printed biomolecules
WO2010044083A2 (en) * 2008-10-14 2010-04-22 Dublin City University A multi-well plate for biological assays

Non-Patent Citations (9)

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Title
CHANDRA KUMAR DIXIT ET AL: "Development of a High Sensitivity Rapid Sandwich ELISA Procedure and Its Comparison with the Conventional Approach", ANALYTICAL CHEMISTRY, vol. 82, no. 16, 15 August 2010 (2010-08-15), pages 7049 - 7052, XP055048836, ISSN: 0003-2700, DOI: 10.1021/ac101339q *
DAN ZHENG ET AL: "Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene", TALANTA, vol. 99, 15 May 2012 (2012-05-15), pages 22 - 28, XP055093586, ISSN: 0039-9140, DOI: 10.1016/j.talanta.2012.05.014 *
DAN ZHENG ET AL: "Rapid and simple preparation of a reagentless glucose electrochemical biosensor", THE ANALYST, vol. 137, no. 16, 1 January 2012 (2012-01-01), pages 3800, XP055048865, ISSN: 0003-2654, DOI: 10.1039/c2an35128e *
DIXIT CHANDRA KUMAR ET AL: "Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 439 - 445, XP009166031, ISSN: 1750-2799 *
S K VASHIST ET AL: "Rapid Immunodiagnostic Kits based on proprietary 1-step chemistry for covalent and leach-proof antibody immobilisation", MIKROSYSTEMTECHNIK - KONGRESS 2013, AACHEN, GERMANY, 14-16 OCT. 2013; POSTER 1.13, 15 October 2013 (2013-10-15), http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/vashist-rapid-immunodiagnostic-kits, pages 384 - 387, XP055093601 *
SANDEEP KUMAR VASHIST ET AL: "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay", THE ANALYST, vol. 136, no. 21, 1 January 2011 (2011-01-01), pages 4431, XP055048842, ISSN: 0003-2654, DOI: 10.1039/c1an15325k *
SANDEEP KUMAR VASHIST: "A sub-picogram sensitive rapid chemiluminescent immunoassay for the detection of human fetuin A", BIOSENSORS AND BIOELECTRONICS, vol. 40, no. 1, 1 February 2013 (2013-02-01), pages 297 - 302, XP055093588, ISSN: 0956-5663, DOI: 10.1016/j.bios.2012.07.067 *
SANDEEP KUMAR VASHIST: "Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications", DIAGNOSTICS, vol. 2, no. 3, 27 August 2012 (2012-08-27), pages 23 - 33, XP055093581, DOI: 10.3390/diagnostics2030023 *
YANG LUO ET AL: "Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay", JOURNAL OF TRANSLATIONAL MEDICINE, BIOMED CENTRAL, LONDON, GB, vol. 10, no. 1, 6 February 2012 (2012-02-06), pages 24, XP021118747, ISSN: 1479-5876, DOI: 10.1186/1479-5876-10-24 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105498706A (en) * 2015-12-09 2016-04-20 广东研捷医药科技有限公司 Preparation method for protein A immunoaffinity column

Also Published As

Publication number Publication date Type
WO2014056896A2 (en) 2014-04-17 application
EP2903740A2 (en) 2015-08-12 application

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