WO2014033796A1 - 液体制御方法 - Google Patents
液体制御方法 Download PDFInfo
- Publication number
- WO2014033796A1 WO2014033796A1 PCT/JP2012/005572 JP2012005572W WO2014033796A1 WO 2014033796 A1 WO2014033796 A1 WO 2014033796A1 JP 2012005572 W JP2012005572 W JP 2012005572W WO 2014033796 A1 WO2014033796 A1 WO 2014033796A1
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- Prior art keywords
- liquid
- flow path
- blood
- collected
- suction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
- A61B5/150229—Pumps for assisting the blood sampling
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
- A61B5/150221—Valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
- A61B5/150236—Pistons, i.e. cylindrical bodies that sit inside the syringe barrel, typically with an air tight seal, and slide in the barrel to create a vacuum or to expel blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
- A61B5/150244—Rods for actuating or driving the piston, i.e. the cylindrical body that sits inside the syringe barrel, typically with an air tight seal, and slides in the barrel to create a vacuum or to expel blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150992—Blood sampling from a fluid line external to a patient, such as a catheter line, combined with an infusion line; blood sampling from indwelling needle sets, e.g. sealable ports, luer couplings, valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/155—Devices specially adapted for continuous or multiple sampling, e.g. at predetermined intervals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01T—MEASUREMENT OF NUCLEAR OR X-RADIATION
- G01T7/00—Details of radiation-measuring instruments
- G01T7/02—Collecting means for receiving or storing samples to be investigated and possibly directly transporting the samples to the measuring arrangement; particularly for investigating radioactive fluids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/157—Devices characterised by integrated means for measuring characteristics of blood
Definitions
- the present invention relates to a liquid control method in a liquid sampling apparatus.
- a blood collection device that collects blood, that is, collects blood
- the blood collection device is used for quantitative analysis in nuclear medicine diagnosis (for example, PET (Positron Emission Tomography)).
- nuclear medicine diagnosis in order to quantitatively analyze biological function information such as neuroreceptor concentration and tumor metabolism, it is necessary to measure the time-dependent change in drug concentration in arterial blood plasma, that is, radioactivity concentration.
- the following methods are employed as automatic blood collection devices for measuring blood radioactivity concentration (see, for example, Patent Documents 1 and 2 and Non-Patent Documents 1 and 2). These devices are used for measuring the radioactivity concentration in arterial blood of small animals (eg, mice and rats). Note that the automatic blood collection device of Patent Document 1 has a different purpose from other methods.
- Non-Patent Document 1 blood radioactivity concentration is measured by installing a radiation detector in the form of sandwiching a catheter in the middle of a catheter inserted into an artery.
- the diode has an elongated shape with a length of 30 [mm], and by piping a tube containing blood along the long side direction, the detectable area is increased and ⁇ + ray detection efficiency is ensured. ing.
- a syringe pump is connected to the other end of the catheter, blood is drawn by pulling the syringe pump at a constant speed, and the flow rate is obtained from the speed, and the volume is calculated from the inner diameter of the catheter to measure the radioactivity concentration.
- Non-Patent Document 2 In Non-Patent Document 2, as shown in FIG. 7, blood is returned to the vein V from the other end of the catheter C inserted into the artery A, and the LYSO detector D and the peristaltic pump P are installed in the middle of the catheter C.
- the ⁇ -rays generated by the disappearance of ⁇ + rays in the arterial blood flowing inside the catheter C enter the LYSO detector D and shine, and the light is counted in the collection box B through the optical fiber F.
- the flow rate of blood is controlled by the peristaltic pump P, and the control PC calculates the volume from the flow rate and the inner diameter of the catheter to measure the radioactivity concentration.
- Patent Document 1 The flow path is switched at the 5-way joint, and the discharge of blood and washing liquid and the collection of blood are repeated.
- Patent Document 2 The present applicant has previously proposed a technique related to an automatic blood collection apparatus, and Patent Document 2 includes a process of pushing blood back to an animal with a physiological saline containing heparin.
- Non-Patent Document 1 Since blood is not returned to the animal simply by pulling with a syringe pump, blood loss exceeds the allowable range in long-term measurement. Therefore, long-term blood collection is impossible. In order to reduce blood loss and reduce the burden on the animal, it is necessary to push the blood back to the animal.
- Non-Patent Document 2 There is no blood loss because blood is returned from the veins to the body.
- animal surgery requires insertion of a catheter not only into an artery but also into a vein, the labor of the operation increases. It also increases the risk of surgery failure.
- Patent Document 1 The blood is pushed back to the animal during the waiting time between collections, but it is fixed while pushed back, so there is a possibility that the blood will solidify there. This is not a problem limited to only blood, and there is a possibility that the liquid may coagulate even when a liquid other than blood is collected.
- Patent Document 2 There is a problem that mixing starts from the interface where the physiological saline containing heparin comes into contact, and the blood is diluted.
- the present invention has been made in view of such circumstances, and an object thereof is to provide a liquid control method capable of preventing the solidification of the liquid to be collected.
- the present invention has the following configuration. That is, the liquid control method according to the present invention is a liquid control method in a liquid sampling apparatus including a flow path having a predetermined length and suction / discharge means connected to the flow path to push and pull the liquid, A fluid composed of at least one of liquid and gas is filled in the flow path on the suction / discharge means side of the liquid to be collected, and the flow on the suction / discharge means side is in a waiting time between collection and the next collection. The movement of the liquid to be collected is controlled by pushing and pulling the fluid filled in the path by suction and discharge means.
- the liquid is collected regardless of the state of the liquid supply source (collection source) by actively pushing and pulling the liquid to be collected by the suction and discharge means.
- the liquid to be collected is blood
- blood can be collected even if the blood pressure decreases due to the physiological state of the animal.
- fluid can be collected even in situations where the animal's blood pressure has decreased.
- the liquid sampling apparatus includes a flow path having a predetermined length, the length is predetermined and the volume is known. For example, a specified volume amount can be collected without measurement by an optical measuring means).
- the liquid sampling apparatus can be reduced in size, and can be installed in the vicinity of the liquid sampling source (eg, animal). As a result, it is possible to reduce the dead volume (which indicates an invalid volume) and eliminate the causes of distortion of the concentration waveform such as delay and dispersion.
- a fluid consisting of at least one of a liquid and a gas is filled in the flow path closer to the suction and discharge means than the liquid to be collected.
- the fluid to be collected is controlled by pushing and pulling the fluid filled in the flow path on the suction / discharge means side during the waiting time between the suction and discharge means. Since the liquid to be collected continues to move by filling the flow path with the fluid and pushing and pulling the fluid by the suction and discharge means during the standby time, the liquid to be collected does not solidify in the flow path and the liquid to be collected is solidified. Can be prevented.
- the liquid control method it is preferable to control the reciprocating movement of the liquid to be collected by repeatedly pushing and pulling the fluid filled in the flow path on the suction / discharge means side. Since the liquid to be collected moves back and forth during the waiting time, the liquid to be collected can be further prevented from solidifying.
- the fluid examples include both liquid and gas, only gas, or only liquid as described below. That is, in the first example, the fluid is a liquid and a gas, the liquid is filled in the flow path on the suction / discharge means side, and the liquid filled in the flow path on the suction / discharge means side is between the liquid to be collected and the liquid to be collected. Gas is sandwiched in the flow path, and the liquid filled in the flow path on the suction and discharge means side is pushed and pulled by the suction and discharge means during the standby time, thereby controlling the movement of the liquid to be collected. .
- the liquid to be collected is accurately Can move. Therefore, it is possible to stop the liquid to be collected immediately before a desired position using a flow path having a predetermined length. In this way, the liquid to be collected continues to move by pushing and pulling the liquid / gas by the suction and discharge means during the standby time, so that the liquid to be collected does not solidify in the flow path and prevents the liquid to be collected from solidifying. can do.
- the former liquid and the latter (collection target) liquid are brought into contact and mixed. It is possible to prevent the liquid to be collected from being diluted by mixing.
- the fluid is a gas
- the gas is filled in the flow path on the suction / discharge means side of the liquid to be collected, and the gas filled in the flow path on the suction / discharge means side is filled in the standby time.
- the movement of the liquid to be collected is controlled by being pushed and pulled by the suction / discharge means. Since the liquid to be collected continues to move by filling the flow path with the gas and pushing and pulling the gas by the suction and discharge means during the standby time, the liquid to be collected does not solidify in the flow path and the liquid to be collected is solidified. Can be prevented. Further, since only the gas is used as the medium without using other liquids, it is possible to prevent the liquid to be collected from coming into contact with other liquids and to mix them, and the liquid to be collected is diluted by mixing. This can also be prevented.
- the fluid is a liquid
- the liquid is filled in the flow path on the suction / discharge means side of the liquid to be collected, and the liquid filled in the flow path on the suction / discharge means side is sucked and discharged in the standby time.
- the movement of the liquid to be collected is controlled by being pushed and pulled by the means.
- the liquid to be collected continues to move by filling the flow path with the liquid and pushing and pulling the liquid by the suction and discharge means during the standby time, so that the liquid to be collected does not solidify in the flow path and the liquid to be collected is solidified. Can be prevented.
- the liquid to be collected may be mixed with the liquid (to be a medium).
- the liquid to be collected And the liquid (which serves as a medium) can be prevented from contacting and mixing.
- the liquid filled in the flow path on the suction / discharge means side is a liquid different from the liquid to be collected, and the flow path on the suction / discharge means side It is preferable to clean the inside of the flow path by moving the filled liquid in the flow path.
- the liquid filled in the flow path on the side of the suction / discharge means moves in the flow path to clean the flow path, thereby also having an effect of cleaning the liquid to be collected remaining in the flow path.
- an example of the liquid to be collected is blood.
- the liquid collection device that performs the liquid control method is a device for collecting blood (blood collection device).
- blood collection device As long as it is a liquid to be collected, it is not limited to blood, but is used for physiological fluids other than blood (for example, fluid containing lymph fluid or protein), fluid containing a fluorescent agent, or an analyzer. A mixed solution or the like may be used.
- the volume of the gas is preferably in the range of 2 [ ⁇ L] to 10 [ ⁇ L]. Since the volume of the control limit gas at the lower limit is 1 [ ⁇ L], 2 [ ⁇ L], which is twice that volume, is the lower limit. If it is less than the control limit, the volume of the gas will be too small, and when the fluid is liquid or gas, when the gas is sandwiched between the liquid and the liquid to be collected, There is a possibility that the liquid that is compressed in the flow path on the suction / discharge means side and the liquid to be collected come into contact with each other. Conversely, when the gas volume exceeds 10 [ ⁇ L], control becomes impossible, and the accuracy of controlling the movement of the liquid to be collected decreases.
- the liquid to be collected continues to move by pushing and pulling the fluid by the suction and discharge means during the standby time, so that the liquid to be collected does not solidify in the flow path and Solidification of the liquid can be prevented.
- FIG. 3 is a schematic view for sequentially explaining a series of blood collection operations according to the first embodiment.
- FIG. 3 is a schematic view for sequentially explaining a series of blood collection operations according to the first embodiment.
- It is the schematic of the liquid sampling device which concerns on a modification.
- It is the schematic of the liquid sampling device which concerns on the further modification.
- It is the schematic of the blood collection apparatus at the time of the system which returns the blood to the vein from the other end of the catheter inserted in the conventional artery.
- FIG. 1 is a schematic diagram of a blood collection apparatus according to each embodiment.
- Example 1 including Example 2 to be described later, blood will be described as an example of the liquid to be collected, and a blood collection device will be described as an example of the liquid collection device.
- the blood collection apparatus 1 collects blood to be collected in time series.
- a container 10 for collecting the collected blood is installed around the blood collection apparatus 1.
- the radioactivity concentration in the arterial blood of a small animal for example, a mouse or a rat
- centrifugation is performed by rotating the container 10 with a centrifuge (not shown).
- blood since blood is centrifuged, plasma is separated, and radiation contained in the plasma and blood cells separated from each other is measured.
- Blood collection apparatus 1 corresponds to the liquid collection apparatus in the present invention.
- the blood collection apparatus 1 is connected to a flow path having a predetermined length (in each embodiment, the first flow path 2, the second flow path 3 and the third flow path 4) and the third flow path 4 among the flow paths.
- a suction / discharge mechanism 5 that pushes and pulls blood to be collected, a connection terminal 6 that branches a plurality of channels (two in each example), and a channel (first channel 2 and second channel in each example)
- a pinch valve 7 that opens and closes the flow path 3) and a dropping port 8 that is connected to the branched flow path (second flow path 3 in each embodiment) and drops the collected blood to be collected. .
- the blood collection device 1 includes a dropping port moving mechanism 9 that moves the dropping port 8 in order to change the position at which the separated and collected blood to be collected is dropped.
- the first flow path 2, the second flow path 3 and the third flow path 4 correspond to the flow paths in the present invention, and the suction / discharge mechanism 5 corresponds to the suction / discharge means in the present invention.
- the liquid supply source (small animal in each embodiment) side is upstream, and the dripping port 8 side is downstream. Therefore, in this specification, the flow path located upstream from the connection terminal 6 is defined as the first flow path 2, and the flow path located downstream from the connection terminal 6 and upstream from the dropping port 8 is defined as the second flow path 3. And Further, the suction / discharge mechanism 5 is connected to the third flow path 4 when a flow path different from the second flow path 3 for connecting the dropping port 8 is used as the third flow path 4. 5 is constituted.
- a tube having a small cross-sectional area (that is, a thin diameter) is used in order to reduce the amount of blood collected.
- Shirascon registered trademark
- Each tube (Shirasukon tube) is used.
- the first flow path 2 and the second flow path 3 have lengths so that the movement of the liquid (blood in each embodiment) can be controlled only by pushing and pulling by the suction / discharge mechanism 5. It is decided in advance. Of course, the length of the third flow path 4 connected to the suction / discharge mechanism 5 may be determined in advance.
- the suction / discharge mechanism 5 uses a syringe pump.
- a syringe pump for the suction / discharge mechanism 5
- liquid (blood) can be collected by pushing and pulling a few [ ⁇ L] of liquid (blood in each embodiment) with high speed and accuracy.
- the suction / discharge mechanism 5 is a syringe pump capable of accurately pushing and pulling the liquid (blood), and the first flow path 2 and the second flow path 3 are known in advance in length and cross-sectional area. Since the volume can be calculated from the above, the blood flowing in the first flow path 2 or the second flow path 3 is moved by the volume pushed and pulled by the suction / discharge mechanism 5.
- liquid and gas are used as the medium (fluid) by pushing and pulling.
- the gas by pushing and pulling may be air, or any gas that does not react with blood or a heparin solution, as exemplified by rare gases such as helium, neon, and argon, or nitrogen gas.
- the liquid by pushing and pulling is not particularly limited, but it is preferable to use a cleaning liquid typified by a heparin solution used for channel cleaning or blood discharge.
- a low-viscosity liquid such as water or mineral oil is preferable in order to increase blood control accuracy.
- reference character L in FIG. 1 and FIG. 3 and FIG. 4 to be described later is a liquid different from blood to be collected.
- Reference sign BL 1 in FIG. 3 to be described later and reference signs BL 1 , BL 2 and BL 3 in FIG. 4 to be described later are blood to be collected.
- the liquid L is illustrated with stippled hatching
- the gas G is illustrated with white paint.
- the first flow path 2, the second flow path 3 and the third flow path 4 are connected by a connection terminal 6.
- the connection terminal 6 uses a block made of PDMS resin (Polydimethylsiloxane) having a minute flow path hole.
- the first flow path 2, the second flow path 3 and the third flow path 4 are connected to the respective holes.
- the connection terminal 6 branches the first flow path 2 into two second flow paths 3 and a third flow path 4.
- a pinch valve 7 for pinching a tube is used as the opening / closing means for opening and closing the flow path.
- the pinch valve 7 applies a pressure from the outside of the tube (see “blocking portion 7a” in FIG. 1 and FIG. 3 and FIG. 4 described later), and the flow path (the first flow path 2 and the second flow path) formed of the tube. 3) is closed, and the flow path (the first flow path 2 and the second flow path 3) made of the tube is opened by releasing the pressure from the outside of the tube.
- the pinch valve 7 is comprised so that when one is opened among two flow paths (1st flow path 2 and 2nd flow path 3) which consist of a tube, the other is closed. . Accordingly, the pinch valve 7 closes the second flow path 3 when the first flow path 2 is opened, and conversely closes the first flow path 2 when the second flow path 3 is opened. Switch.
- the dropping port 8 for dropping the collected blood is positioned on the container 10 for storing the collected blood, and the dropping position is changed by the dropping port moving mechanism 9.
- the dropping port moving mechanism 9 uses an electric slider by a stepping motor, and moves the dropping port 8 forward, backward, left, and right (horizontal direction) by the electric slider.
- the pinch valve 7 so that the distance between the pinch valve 7 and the sampling source (small animal in each embodiment) in the flow path is in the range of 10 [cm] to 20 [cm]. In order to make it easy to connect the pinch valve 7 and prevent the liquid from diffusing, the distance is 15 [cm], which is the center of the range of 10 [cm] to 20 [cm]. It is more preferable to arrange the pinch valve 7 as described above.
- the fluid (liquid L / gas G) is collected on the side closer to the suction / discharge mechanism 5 than the liquid to be collected (blood BL 1 to BL 3 in FIG. 1 and FIGS. 3 and 4 described later). Fill the road. Then, the fluid (liquid L / gas G) filled in the flow path on the suction / discharge mechanism 5 side is pushed / pulled by the suction / discharge mechanism 5 during the standby time between the collection and the next collection. The movement of the liquid (blood BL 1 to BL 3 ) is controlled.
- the fluid is both the liquid L and the gas G
- the liquid L fills the flow path on the suction / discharge mechanism 5 side and the liquid filled in the flow path on the suction / discharge mechanism 5 side.
- Gas G is sandwiched in the flow path between L and the liquid to be collected (blood BL 1 to BL 3 ).
- the liquid L filled in the flow path on the side of the suction / discharge mechanism 5 is pushed / pulled by the suction / discharge mechanism 5 during the standby time to push / pull the gas G, and the liquids to be collected (blood BL 1 to BL 3) ) Move control.
- the volume of the gas G is preferably in the range of 2 [ ⁇ L] to 10 [ ⁇ L].
- the contact between the liquid L filled in the flow path on the side of the suction and discharge means and the liquid to be collected (blood in each embodiment) is prevented, and the movement of the liquid (blood) to be collected is controlled.
- the volume is more preferably 5 [ ⁇ L] to 6 [ ⁇ L], which is the center of the range of 2 [ ⁇ L] to 10 [ ⁇ L].
- FIG. 2 is a flowchart showing a flow of a series of blood collection processes according to each embodiment
- FIGS. 3 and 4 are schematic diagrams sequentially explaining a series of blood collection operations according to the first embodiment. 3 and 4, a predetermined amount of blood to be collected (waste liquid) (reference numeral BL 1 ) drawn in the initial state is illustrated by hatching in the upper right diagonal line, and finally collected blood ( The symbol BL 2 ) is shown by hatching in the upper left diagonal line, and the other blood (reference symbol BL 3 ) is shown in black.
- waste liquid reference numeral BL 1
- the symbol BL 2 is shown by hatching in the upper left diagonal line
- the other blood reference symbol BL 3
- the liquid L is filled in the flow path on the suction / discharge mechanism 5 side, and the gas G is filled in the flow path on the collection source (small animal in each embodiment) side that is the liquid supply source.
- Step S1 in FIG. 3 shows an initial state before blood collection. Since animals gas such as air when pushed back all the blood will die enters the animal body, it is slightly left blood BL 1 outside the body by sucking a predetermined amount of blood BL 1 in order to prevent it. More specifically, the first flow path 2 is opened by the pinch valve 7 and the second flow path 3 is closed, and the suction / discharge mechanism 5 sucks a predetermined amount of blood BL 1 before collection. Set to the initial state. By sucking the predetermined amount of blood BL 1 , the gas G is sandwiched between the liquid L filled in the flow path on the suction / discharge mechanism 5 side and the sucked blood BL 1 .
- Step S2 Collection time? If the blood collection time has not been reached, the reciprocation of step S3 is performed. When the blood collection time is reached, the first suction in step S4 is performed.
- Step S3A in FIG. 3 shows blood aspiration.
- the liquid L filled in the flow path on the side of the suction / discharge mechanism 5 is sucked by the suction / discharge mechanism 5 to suck the gas G and suck blood.
- Step S3B of FIG. 3 shows blood pushback.
- the gas G is pushed back, and the blood is pushed back.
- steps S3A and S3B the liquid L filled in the flow path on the side of the suction / discharge mechanism 5 is pushed and pulled by the suction / discharge mechanism 5 during a waiting time between the collection and the next collection.
- Push and pull G to control the movement of blood to be collected.
- the push-pull of the liquid L filled in the flow path on the suction / discharge mechanism 5 side is repeated, whereby the gas G The push-pull is repeated to control the reciprocation of blood to be collected.
- the first flow path 2 is open and the second flow path 3 is closed.
- the amount to be pushed and pulled is not particularly limited, but may be any amount that can prevent coagulation.
- the amount of blood to be pushed and pulled is 1 [ ⁇ L] to 2 [2]. ⁇ L].
- the suction / discharge mechanism 5 repeats a minute push-pull of 1 [ ⁇ L] to 2 [ ⁇ L] so that the blood continues to move.
- Step S4 First suction At the start of blood collection, the suction / discharge mechanism 5 is pulled to draw blood BL 2 and BL 3 from the animal. More specifically, the pinch valve 7 opens the first flow path 2 and closes the second flow path 3, followed by a predetermined amount of blood BL 1 to be collected drawn in the initial state. Then, the blood BL 2 , BL 3 is further sucked by the suction / discharge mechanism 5 until the blood BL 1 is located at the connection terminal 6. As described in step S3, the blood L is sucked by sucking the liquid L / gas G.
- Step S5 First discharge Since blood BL 2 in the body is to be collected at the time of blood collection, the blood BL 1 that has gone out of the body (that is, a predetermined amount of blood BL 1 to be collected drawn in the initial state) is discarded. Te, to collect the blood BL 2 then to come. For this purpose, the blood BL 1 to be discarded is drawn from the connection terminal 6 to the suction / discharge mechanism 5 side (right side in FIG. 3), and the blocking portion 7 a of the pinch valve 7 is moved from the second flow path 3 to the first flow path 2. Then, the suction / discharge mechanism 5 is pushed to discharge the blood BL 1 from the dripping port 8 via the second flow path 3.
- the pinch valve 7 closes the first flow path 2 and opens the second flow path 3 so that a predetermined amount drawn in the initial state is obtained.
- the blood BL 1 to be collected is pushed back and discharged from the dropping port 8 via the second flow path 3 and discharged.
- the dropping inlet 8 is moved over the container 10 to the outside by dropping inlet moving mechanism 9, discharges the blood BL 1.
- the blood is pushed back by pushing back the liquid L and the gas G.
- step S5 of FIG. 4 the gas G flows into the dropping port 8 connected to the second flow path 3, and the liquid L and the blood BL 2 directly flow at the branch point at the connection terminal 6. Contact.
- the blocking portion 7a of the pinch valve 7 is in the first flow path 2, and the first flow path 2 is closed and the second flow path 3 is opened, the liquid L is mixed into the first flow path 2. It flows without fail to the second flow path 3. Therefore, mixing of the liquid L can be prevented.
- a heparin solution is used for the liquid L, an interface is formed between the heparin solution and blood, so that the mixing of the liquid L can be further prevented.
- the gas L is sucked into the flow path between the liquid L and the blood BL 2 by sucking the liquid L / gas G by the suction / discharge mechanism 5 until the gas G is located at the branch point at the connection terminal 6. It is caught.
- Step S ⁇ b> 6 Second suction
- the dropping port moving mechanism 9 moves the dropping port 8 onto the container 10 to switch the closing portion 7 a of the pinch valve 7 from the first channel 2 to the second channel 3.
- the blood BL 2 is drawn from the connection terminal 6 to the suction / discharge mechanism 5 side (right side in FIG. 3). More specifically, after the first discharge in step S5, the pinch valve 7 opens the first flow path 2 and closes the second flow path 3, and the blood BL 2 to be collected is connected to the connection terminal.
- the blood BL 2 is sucked by the suction / discharge mechanism 5 until it is positioned closer to the suction / discharge mechanism 5 than 6. At this time, the blood BL 3 Following the aspiration of blood BL 2 is also sucked.
- blood is sucked by sucking the liquid L and gas G.
- Step S ⁇ b> 7 The second discharge pinch valve 7 is switched from the second flow path 3 to the first flow path 2, and the suction / discharge mechanism 5 is pressed to drop the blood BL 2 into the container 10 from the dropping port 8. More specifically, after the second suction in step S6, the pinch valve 7 draws out the second suction in step S6 with the first flow path 2 closed and the second flow path 3 opened. The blood BL 2 to be collected is pushed back and discharged from the dropping port 8 through the second flow path 3 and collected. As described in Steps S3 and S5, the blood is pushed back by pushing back the liquid L and the gas G.
- step S5 even when the collected blood BL 2 by the second discharge in the step S7, preferably stopping gas G in dropping inlet 8.
- step S5 in order to create and push out the blood droplets to be collected, it is more preferable to push out 0.5 V, which is half the total volume V, from the dropping port 8 together. Then, after dropping, if 0.5 G of excessively extruded gas G (air in this case) is sucked from the dripping port 8 and returns to the state of step S9 described later, the sucked 0.5V gas G When combined with 0.5V + 0.5V, the total volume returns to V.
- the gas L is sucked into the flow path between the liquid L and the blood BL 3 by sucking the liquid L / gas G by the suction / discharge mechanism 5 until the gas G is located at the branch point at the connection terminal 6. It is caught.
- Step S8 Is it collected?
- the process returns to step S2 after pushing back in step S9, and the same processes in steps S2 to S8 are repeated.
- a series of blood collection processing ends.
- Step S9 Push back After dropping, the closing portion 7a of the pinch valve 7 is switched from the first flow path 2 to the second flow path 3, the suction / discharge mechanism 5 is pushed to push the blood BL 3 back to the animal, and the process returns to Step S2. More specifically, after the second discharge in step S7, the blood to be collected by the suction / discharge mechanism 5 in a state where the first flow path 2 is opened by the pinch valve 7 and the second flow path 3 is closed. The BL 3 is pushed back to the animal from which it was collected to the initial state.
- Step S9 the determination of the sampling time in step S2, the reciprocating movement in step S3, the first suction in step S4, the first discharge in step S5, the second suction in step S6, and the second in step S7. Repeat two discharges.
- Steps S3, S5, and S7 the blood is pushed back by pushing back the liquid L and the gas G.
- the liquid to be collected (blood in each embodiment) is actively pushed and pulled by the suction / discharge mechanism 5.
- Liquid (blood) can be collected regardless of the situation of the (blood) supply source (collection source).
- the blood collection device 1 includes a channel having a predetermined length (in each embodiment, the first channel 2, the second channel 3, and the third channel 4), so that the length is defined in advance.
- the liquid collection device blood collection device 1 in each embodiment
- the liquid collection device can be reduced in size, and can be installed in the vicinity of the liquid collection source (animal in each embodiment).
- the dead volume which indicates an invalid volume
- eliminate the causes of distortion of the concentration waveform such as delay and dispersion.
- a fluid consisting of at least one of the liquid L and the gas G is used as a liquid to be collected (the blood BL 1 in FIGS. 1, 3 and 4).
- ⁇ BL 3 also filled in the flow path of the suction and discharge mechanism 5 side from, collected fluid filled in the flow path of the suction and discharge mechanism 5 side to the waiting time is between subsequent sampled (liquid L ⁇ gas G)
- the liquid (blood) to be collected is controlled by being pushed and pulled by the suction / discharge mechanism 5.
- the liquid (blood) to be collected continues to move.
- the liquid (blood) to be collected can be prevented from coagulating without being solidified in the flow path.
- both liquid L and gas G are employed as fluids. That is, in the first embodiment, the fluid is the liquid L and the gas G, and the liquid L fills the flow path on the suction / discharge mechanism 5 side, and the liquid L filled in the flow path on the suction / discharge mechanism 5 side Gas G is sandwiched between the liquids to be collected (blood BL 1 to BL 3 ) in the flow path, and the liquid L filled in the flow path on the suction / discharge mechanism 5 side is pushed by the suction / discharge mechanism 5 during the standby time. By pulling, the gas G is pushed and pulled to control the movement of the liquid (blood) to be collected.
- the liquid (blood) to be collected can be moved with high accuracy. Therefore, it is possible to stop the liquid (blood) to be collected immediately before a desired position (for example, a position where blood is supplied by pushing back in step S3) using a flow path having a predetermined length. In this way, the liquid (blood) to be collected continues to move by pushing and pulling the liquid L / gas G by the suction / discharge mechanism 5 during the standby time, so that the liquid (blood) to be collected does not solidify in the flow path.
- the coagulation of the liquid (blood) to be collected can be prevented. Further, by sandwiching the gas G between the liquid L filled in the flow path on the suction / discharge mechanism 5 side and the liquid (blood) to be collected, the former liquid L and the latter (collection target) liquid (blood) ) Can be prevented from contacting and mixing, and the liquid (blood) to be collected can also be prevented from thinning due to mixing.
- the above-described push-pull of the gas G is repeated by repeating the push-pull of the liquid L filled in the flow path on the suction / discharge mechanism 5 side to control the reciprocating movement of the liquid (blood) to be collected.
- the liquid (blood) to be collected reciprocates during the waiting time, the liquid (blood) to be collected can be further prevented from coagulating.
- the liquid L filled in the flow path on the suction / discharge mechanism 5 side is a liquid different from the liquid (blood) to be collected, and is suctioned It is preferable to clean the inside of the flow path by moving the liquid L filled in the flow path on the discharge mechanism 5 side in the flow path.
- the liquid L uses a heparin solution, water, or mineral oil to clean the inside of the flow path. The effect of washing the liquid (blood) to be collected remaining in the flow path by washing the flow path by moving the liquid L filled in the flow path on the suction / discharge mechanism 5 side in the flow path. Also play.
- the liquid collection device that performs the liquid control method is a device for collecting blood, that is, the blood collection device 1.
- the volume of the gas G is preferably in the range of 2 [ ⁇ L] to 10 [ ⁇ L]. Since the volume of the control limit gas at the lower limit is 1 [ ⁇ L], 2 [ ⁇ L], which is twice that volume, is the lower limit. If it is less than the control limit, the volume of the gas G becomes too small, and when the fluid is the liquid L and the gas G, the gas G is sandwiched between the liquid L and the liquid to be collected (blood). In some cases, when pushed and pulled, the gas L is compressed and the liquid L filled in the flow path on the suction and discharge mechanism 5 side may come into contact with the liquid (blood) to be collected. On the other hand, when the volume of the gas G exceeds 10 [ ⁇ L], control becomes impossible, and the accuracy of controlling the movement of the liquid (blood) to be collected decreases.
- Embodiment 2 of the present invention will be described with reference to the drawings.
- both liquid and gas are employed as the fluid.
- only one of the liquid and the gas is employed as the fluid. 3 and FIG. 4 are used.
- the gas G is not particularly limited and may be air as in the first embodiment, or reacts with blood or a heparin solution, as exemplified by rare gases such as helium, neon, and argon, or nitrogen gas. Any gas that does not work.
- the liquid L is not particularly limited, and is a heparin solution, water, or mineral oil as in the first embodiment.
- steps S1 to S9 Since the flowchart of steps S1 to S9 is the same as that of the first embodiment, the description thereof is omitted.
- the gas L is filled in the flow path on the suction / discharge mechanism 5 side with respect to the liquid (blood) to be collected, and the gas filled in the flow path on the suction / discharge mechanism 5 side in the standby time
- the liquid (blood) to be collected is controlled by being pushed and pulled by the suction / discharge mechanism 5.
- the liquid (blood) to be collected continues to move by filling the gas G into the flow path and pushing and pulling the gas G by the suction / discharge mechanism 5 during the standby time, so the liquid (blood) to be collected solidifies in the flow path. Without coagulation of the liquid (blood) to be collected.
- the liquid L When the fluid is the liquid L, the liquid L is filled in the flow path on the suction / discharge mechanism 5 side with respect to the liquid (blood) to be collected, and the liquid is filled in the flow path on the suction / discharge mechanism 5 side in the standby time.
- the liquid to be collected (blood) is controlled to move by pushing and pulling L by the suction / discharge mechanism 5. Since the liquid (blood) to be collected continues to move by filling the liquid L into the flow path and pushing and pulling the liquid L by the suction / discharge mechanism 5 during the standby time, the liquid L to be collected does not solidify in the flow path. The coagulation of the liquid (blood) to be collected can be prevented.
- the liquid (blood) to be collected may be mixed with the liquid L (to be a medium), but a liquid (for example, a heparin solution) that forms an interface with the liquid (blood) to be collected is used as the medium.
- a liquid for example, a heparin solution
- it is possible to prevent the liquid (blood) to be collected and the liquid L (to be a medium) from coming into contact with each other and mixing.
- the present invention is not limited to the above embodiment, and can be modified as follows.
- blood has been described as an example of the collection target liquid.
- the liquid is not limited to blood but may be a physiological fluid other than blood (for example, a fluid containing lymph or protein), a fluid containing a fluorescent agent, a mixed solution used in an analyzer, or the like.
- the blood collection device 1 is shown in FIG. 1, but is not limited to the structure shown in FIG. 1. If the liquid sampling apparatus includes a flow path having a predetermined length and a suction / discharge means (suction / discharge mechanism) connected to the flow path to push and pull the liquid, the liquid sampling as shown in FIG. 5 or FIG. It can be applied to the device.
- suction / discharge means suction / discharge mechanism
- the blood is accommodated in a state where an anticoagulant for preventing the occurrence of blood coagulation is applied to the container 11, or as described in the modification (1) described above.
- the liquid to be collected (illustrated by hatching in the upper right oblique line) is sucked in the state where the first flow path 2 is inserted into the container 11, and the waiting time is reached.
- the liquid G to be collected may be pushed and pulled by the liquid L / suction and discharge mechanism 5 filled in the flow path on the suction and discharge mechanism 5 side to control the movement of the liquid to be collected.
- the medium (fluid) by pushing and pulling may be only the liquid L or only the gas G.
- the liquid to be collected (shown by hatching in the upper right diagonal line) is sucked in a state where a single flow path (tube) that does not branch is inserted into the container 11, and the waiting time is reached.
- the liquid G to be collected may be pushed and pulled by the liquid L / suction and discharge mechanism 5 filled in the flow path on the suction and discharge mechanism 5 side to control the movement of the liquid to be collected.
- the flow path is removed from the container 11 and discharged from the dropping port of the flow path to the collection container (not shown in FIG. 6).
- the flow path serves as both a flow path for sucking liquid and a flow path for dropping.
- the liquid to be collected can be stopped immediately before the position of the supply source, in the case of the structure shown in FIG. 6, an opening / closing means such as the pinch valve 7 is not necessarily required.
- the medium (fluid) by pushing and pulling may be only the liquid L or only the gas G.
- the flow path is a tube, but may be a groove provided in a substrate or the like, for example.
- the opening and closing means for opening and closing the flow path is opened and closed by using a valve of a type that allows liquid to pass inside other than the pinch valve.
- the opening / closing means for opening and closing the flow path is a pinch valve.
- a valve that allows liquid to pass inside other than the pinch valve may be.
- the first flow path and the second flow path are formed by the Shirasucon tube only at the portion pinched by the pinch valve, but it is not limited to the Shirasucon.
- Each flow path may be formed of a tube made of a rubber material having a soft resilience such as silicone, tygon, or polyurethane. Further, the material may be changed for only the portion to be pinched, and the same material may be used for the first flow path and the second flow path, or the entire first to third flow paths.
- the reciprocating movement of the liquid to be collected is controlled by repeatedly pushing and pulling the fluid filled in the flow path of the suction / discharge means (suction / discharge mechanism).
- the liquid to be collected may be controlled to move by pushing and pulling the fluid composed of liquid / gas only during the time when the liquid may be solidified.
- the medium (fluid) by pushing and pulling may be only the liquid L or only the gas G.
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Abstract
Description
非特許文献1では、動脈に挿入したカテーテルの途中に、カテーテルを挟み込む形で放射線検出器を設置することで、血中放射能濃度を測定する。ダイオードは、長さが30[mm]の細長い形状を有し、長辺方向に沿って血液が入ったチューブを配管することで、検出可能面積を増加させ、β+線の検出効率を確保している。カテーテルの他端にはシリンジポンプを接続し、シリンジポンプを一定速度で引いて血液を引き出し、さらにその速度から流量を求めてカテーテルの内径から体積を算出して放射能濃度を計測する。
非特許文献2では、図7に示すように動脈Aに挿入したカテーテルCの他端から静脈Vに血液を戻し、カテーテルCの途中にLYSO検出器DおよびペリスタポンプPを設置する。カテーテルC内部を流れる動脈血内のβ+線が対消滅することで発生するγ線がLYSO検出器Dに入射して光り、その光は光ファイバFを収集ボックスBで計数する。血液の流量はペリスタポンプPによって制御され、その流量およびカテーテルの内径から制御PCは体積を算出して放射能濃度を計測する。
5方ジョイントで流路を切り替えて、血液や洗浄液の排出や血液の採取を繰り返す。
本出願人は、自動採血装置に関する技術を以前に提案しており、特許文献2では、ヘパリン(heparin)入り生理食塩水で動物へ血液を押し戻す処理が含まれている。
シリンジポンプで引くだけで動物に血液を戻さないので、長時間の測定では失血が許容範囲を超えてしまう。したがって、長時間の採血は不可能である。失血を減らして動物の負担を軽減するためには、動物へ血液を押し戻す必要がある。
血液を静脈から体内に戻すので失血はない。しかし、動物手術は動脈だけでなく静脈にもカテーテル挿入が必要なので、手術の手間が増える。また手術が失敗するリスクも増える。
採取と次の採取との間である待機時間中に動物へ血液を押し戻すが、押し戻したまま固定するので、そこで血液が固まる可能性が考えられる。これは血液のみに限定した問題でなく、血液以外の液体を採集する場合にも液体が凝固する可能性がある。
ヘパリン入り生理食塩水が接触する界面から混合が始まり、血液が薄まるという問題がある。
すなわち、この発明に係る液体制御方法は、所定の長さを有する流路と、その流路に接続し液体を押し引きする吸引吐出手段とを備えた液体採取装置における液体制御方法であって、液体,気体の少なくともいずれか一方からなる流体を採取対象の液体よりも前記吸引吐出手段側の前記流路に満たし、採取と次の採取との間である待機時間に前記吸引吐出手段側の流路に満たされた前記流体を吸引吐出手段により押し引きすることにより、採取対象の液体を移動制御することを特徴とするものである。
すなわち、最初の一例は、流体は液体および気体であって、当該液体を吸引吐出手段側の流路に満たし、吸引吐出手段側の流路に満たされた当該液体と採取対象の液体との間に気体を流路内に挟み込み、待機時間に吸引吐出手段側の流路に満たされた当該液体を吸引吐出手段により押し引きすることにより気体を押し引きして、採取対象の液体を移動制御する。液体を流路に詰めて、採取対象の液体との間に気体を挟み込んで押し引きすれば、吸引吐出手段による押し引きによって圧縮または膨張される気体の体積が減るので採取対象の液体を精度良く動かすことができる。よって、所定の長さを有する流路を用いて所望の位置の直前に採取対象の液体を止めることもできる。このように、待機時間に吸引吐出手段により液体・気体を押し引きすることで採取対象の液体が動き続けるので、採取対象の液体が流路内で固まることなく、採取対象の液体の凝固を防止することができる。さらに、吸引吐出手段側の流路に満たされた液体と採取対象の液体との間に気体を挟み込むことで、前者の液体と後者の(採取対象の)液体とが接触して混合するのを防止することができ、混合により採取対象の液体が薄まることも防止することができる。
図3のステップS1は採血前の初期状態を示す。動物に血液を全て押し戻すと空気などの気体が動物体内に入って死んでしまうので、それを防止するために血液BL1を所定量だけ吸引することで血液BL1を体外に少し残している。より具体的に説明すると、ピンチバルブ7により、第1流路2を開けて、第2流路3を閉じた状態で、吸引吐出機構5により所定量の血液BL1を吸引することで採取前の初期状態と設定する。この所定量の血液BL1の吸引により、吸引吐出機構5側の流路に満たされた液体Lと吸引された血液BL1との間に気体Gが流路内に挟み込まれる。
採血時刻に到達していない場合には、ステップS3の往復移動を行う。採血時刻に到達した場合には、ステップS4の第1吸引を行う。
図3のステップS3Aは血液の吸引を示す。吸引吐出機構5側の流路に満たされた液体Lを吸引吐出機構5により吸引することにより気体Gを吸引し、血液を吸引する。図3のステップS3Bは血液の押し戻しを示す。吸引吐出機構5側の流路に満たされた液体Lを吸引吐出機構5により押し戻すことにより気体Gを押し戻し、血液を押し戻す。このステップS3AおよびS3Bを行うことで、採取と次の採取との間である待機時間に吸引吐出機構5側の流路に満たされた当該液体Lを吸引吐出機構5により押し引きすることにより気体Gを押し引きして、採取対象の血液を移動制御する。ステップS2に戻り、次の採血時刻に達するまで、ステップS2およびS3の処理を繰り返して行うことで、吸引吐出機構5側の流路に満たされた液体Lの押し引きを繰り返すことにより気体Gの押し引きを繰り返し、採取対象の血液の往復移動を制御する。
採血開始とともに、吸引吐出機構5を引いて動物から血液BL2,BL3を引き出す。より具体的に説明すると、ピンチバルブ7により、第1流路2を開けて、第2流路3を閉じた状態で、初期状態で引き出された所定量の採取対象の血液BL1に引き続いて、当該血液BL1が接続端子6に位置するまで吸引吐出機構5により当該血液BL2,BL3をさらに吸引する。ステップS3でも述べたように液体L・気体Gを吸引することにより、血液を吸引する。
採血時刻に体内にある血液BL2を採取したいので、体外に出ていた血液BL1(すなわち初期状態で引き出された所定量の採取対象の血液BL1)を廃棄して、その後に来る血液BL2を採取する。そのためには、廃棄する血液BL1を接続端子6よりも吸引吐出機構5側(図3中の右側)まで引き出し、ピンチバルブ7の閉塞部7aを第2流路3から第1流路2に切り替え、次に吸引吐出機構5を押して血液BL1を、第2流路3経由で滴下口8から排出する。より具体的に説明すると、ステップS2の第1吸引の後に、ピンチバルブ7により、第1流路2を閉じて、第2流路3を開けた状態で、初期状態で引き出された所定量の採取対象の血液BL1を押し戻して、第2流路3を経由して滴下口8から吐出して排出する。その際に、滴下口移動機構9により滴下口8を容器10の上から外部に移動させてから、血液BL1を排出する。ステップS3でも述べたように液体L・気体Gを押し戻すことにより、血液を押し戻す。
滴下口移動機構9により滴下口8を容器10の上に移動し、ピンチバルブ7の閉塞部7aを第1流路2から第2流路3に切り替え、吸引吐出機構5により血液BL2を接続端子6よりも吸引吐出機構5側(図3中の右側)まで引いてくる。より具体的に説明すると、ステップS5の第1吐出の後に、ピンチバルブ7により、第1流路2を開けて、第2流路3を閉じた状態で、採取対象の血液BL2が接続端子6よりも吸引吐出機構5側に位置するまで吸引吐出機構5により当該血液BL2を吸引する。このとき、血液BL2の吸引に引き続いて血液BL3も吸引される。ステップS3やS4でも述べたように液体L・気体Gを吸引することにより、血液を吸引する。
ピンチバルブ7の閉塞部7aを第2流路3から第1流路2に切り替え、吸引吐出機構5を押して滴下口8から血液BL2を容器10に滴下する。より具体的に説明すると、ステップS6の第2吸引の後に、ピンチバルブ7により、第1流路2を閉じて、第2流路3を開けた状態で、ステップS6の第2吸引で引き出された採取対象の血液BL2を押し戻して、第2流路3を経由して滴下口8から吐出して採取する。ステップS3やS5でも述べたように液体L・気体Gを押し戻すことにより、血液を押し戻す。
引き続いて採血を行う場合には、ステップS9の押し戻しを行った後にステップS2に戻って、同様のステップS2~S8の処理を繰り返して行う。採血を行わない場合には、一連の採血処理を終了する。
滴下後はピンチバルブ7の閉塞部7aを第1流路2から第2流路3に切り替え、吸引吐出機構5を押して血液BL3を動物にまで押し戻してステップS2に戻る。より具体的に説明すると、ステップS7の第2吐出の後に、ピンチバルブ7により、第1流路2を開けて、第2流路3を閉じた状態で、吸引吐出機構5により採取対象の血液BL3を採取元である動物にまで押し戻して初期状態とする。このように、ステップS9の押し戻しの後に、ステップS2の採取時刻の判断,ステップS3の往復移動,ステップS4の第1吸引,ステップS5の第1吐出,ステップS6の第2吸引およびステップS7の第2吐出を繰り返し行う。ステップS3やS5やS7でも述べたように液体L・気体Gを押し戻すことにより、血液を押し戻す。
2 … 第1流路
3 … 第2流路
4 … 第3流路
5 … 吸引吐出機構
BL1~BL3 … 血液
L … 液体
G … 気体
Claims (8)
- 所定の長さを有する流路と、
その流路に接続し液体を押し引きする吸引吐出手段と
を備えた液体採取装置における液体制御方法であって、
液体,気体の少なくともいずれか一方からなる流体を採取対象の液体よりも前記吸引吐出手段側の前記流路に満たし、
採取と次の採取との間である待機時間に前記吸引吐出手段側の流路に満たされた前記流体を吸引吐出手段により押し引きすることにより、採取対象の液体を移動制御することを特徴とする液体制御方法。 - 請求項1に記載の液体制御方法において、
前記吸引吐出手段側の流路に満たされた前記流体の押し引きを繰り返すことにより、採取対象の液体の往復移動を制御することを特徴とする液体制御方法。 - 請求項1または請求項2に記載の液体制御方法において、
前記流体は液体および気体であって、
当該液体を前記吸引吐出手段側の前記流路に満たし、
前記吸引吐出手段側の前記流路に満たされた当該液体と採取対象の液体との間に気体を流路内に挟み込み、
前記待機時間に前記吸引吐出手段側の流路に満たされた当該液体を吸引吐出手段により押し引きすることにより前記気体を押し引きして、前記採取対象の液体を移動制御することを特徴とする液体制御方法。 - 請求項1または請求項2に記載の液体制御方法において、
前記流体は気体であって、
当該気体を前記採取対象の液体よりも前記吸引吐出手段側の前記流路に満たし、
前記待機時間に前記吸引吐出手段側の流路に満たされた当該気体を吸引吐出手段により押し引きすることにより、採取対象の液体を移動制御することを特徴とする液体制御方法。 - 請求項1または請求項2に記載の液体制御方法において、
前記流体は液体であって、
当該液体を前記採取対象の液体よりも前記吸引吐出手段側の前記流路に満たし、
前記待機時間に前記吸引吐出手段側の流路に満たされた当該液体を吸引吐出手段により押し引きすることにより、採取対象の液体を移動制御することを特徴とする液体制御方法。 - 請求項3または請求項5に記載の液体制御方法において、
前記吸引吐出手段側の流路に満たされた前記液体は、前記採取対象の液体とは異なる液体であり、
吸引吐出手段側の流路に満たされた当該液体が流路内を移動することで流路内を洗浄することを特徴とする液体制御方法。 - 請求項1から請求項6のいずれかに記載の液体制御方法において、
前記採取対象の液体は血液であって、
前記液体採取装置は採血するための装置であることを特徴とする液体制御方法。 - 請求項3または請求項4に記載の液体制御方法において、
前記気体の体積は、2[μL]~10[μL]の範囲であることを特徴とする液体制御方法。
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PCT/JP2012/005572 WO2014033796A1 (ja) | 2012-09-03 | 2012-09-03 | 液体制御方法 |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0298365A (ja) * | 1988-06-26 | 1990-04-10 | Terumo Corp | 単針式血漿分離装置および血漿採取装置 |
JPH0599936A (ja) * | 1991-10-08 | 1993-04-23 | Aloka Co Ltd | 高粘性液体の希釈方法 |
JPH08294478A (ja) * | 1995-04-25 | 1996-11-12 | Terumo Corp | 採血装置 |
JP2001116666A (ja) | 1999-10-18 | 2001-04-27 | Eikomu:Kk | 生体液の自動サンプリング装置 |
JP2003530188A (ja) * | 2000-04-12 | 2003-10-14 | メルク エンド カムパニー インコーポレーテッド | 自動化された血液サンプリング装置 |
JP2005153355A (ja) * | 2003-11-26 | 2005-06-16 | Sony Corp | 液体吐出性能維持方法及び液体吐出装置 |
WO2010106580A1 (ja) | 2009-03-19 | 2010-09-23 | 株式会社島津製作所 | 液体採取装置およびその方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1328359C (en) * | 1989-03-27 | 1994-04-12 | Michael D. Mintz | Fluid sample collection and delivery system and methods particularly adapted for body fluid sampling |
US20110060199A1 (en) * | 2005-11-15 | 2011-03-10 | Mark Ries Robinson | Determination of blood pump system performance and sample dilution using a property of fluid being transported |
JP4411661B2 (ja) * | 2007-10-26 | 2010-02-10 | セイコーエプソン株式会社 | 生体物質検出方法 |
-
2012
- 2012-09-03 WO PCT/JP2012/005572 patent/WO2014033796A1/ja active Application Filing
- 2012-09-03 EP EP12883610.3A patent/EP2894454B1/en not_active Not-in-force
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Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0298365A (ja) * | 1988-06-26 | 1990-04-10 | Terumo Corp | 単針式血漿分離装置および血漿採取装置 |
JPH0599936A (ja) * | 1991-10-08 | 1993-04-23 | Aloka Co Ltd | 高粘性液体の希釈方法 |
JPH08294478A (ja) * | 1995-04-25 | 1996-11-12 | Terumo Corp | 採血装置 |
JP2001116666A (ja) | 1999-10-18 | 2001-04-27 | Eikomu:Kk | 生体液の自動サンプリング装置 |
JP2003530188A (ja) * | 2000-04-12 | 2003-10-14 | メルク エンド カムパニー インコーポレーテッド | 自動化された血液サンプリング装置 |
JP2005153355A (ja) * | 2003-11-26 | 2005-06-16 | Sony Corp | 液体吐出性能維持方法及び液体吐出装置 |
WO2010106580A1 (ja) | 2009-03-19 | 2010-09-23 | 株式会社島津製作所 | 液体採取装置およびその方法 |
Non-Patent Citations (3)
Title |
---|
"Blood Sampler twilite", SWISSTRACE, Retrieved from the Internet <URL:http://www.swisstrace.ch/blood-sampler-twilite.html> |
L. CONVERT; G. M. BRASSARD; J. CADORETTE; D. ROULEAU; E. CROTEAU; M. ARCHAMBAULT; R. FONTAINE; R. LECOMTE: "A microvolumetric blood counter for pharmacokinetic PET studies in small animals", IEEE NUCLEAR SCI, vol. 54, no. 1, 2007 |
See also references of EP2894454A4 * |
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EP2894454B1 (en) | 2016-11-09 |
US10463289B2 (en) | 2019-11-05 |
JP6094586B2 (ja) | 2017-03-15 |
EP2894454A4 (en) | 2015-09-30 |
US20150289793A1 (en) | 2015-10-15 |
JPWO2014033796A1 (ja) | 2016-08-08 |
EP2894454A1 (en) | 2015-07-15 |
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